IndraLab

Statements


USP26 affects AR
3 1 | 1 16 7 1
USP26 binds AR.
3 | 9 7 1
3 | 9 4

sparser
"Our study also provides the evidence on the interactions between USP26 and AR in mouse testis, whereby pointing to a potential mechanism."

reach
"Given USP26 interaction with the androgen receptor, we conducted this study to characterize USP26 as a potential target affecting human testicular function and fertility and androgen dependent cancers."

sparser
"As an example, USP26 physically interacts with AR and influences AR ubiquitination and transcriptional activation [ xref ]."

reach
"Our study also provides the evidence on the interactions between USP26 and AR in mouse testis, whereby pointing to a potential mechanism."

reach
"USP26 binds to androgen receptor through three nuclear receptor interaction motifs, and knockdown of USP26 leads to decreased receptor activity in LnCaP and HEK293 cells."

reach
"Usp26 can directly bind and deubiquitinate AR, acting as a co-regulator of the AR by reversing AR activation and degradation by MDM2 ubiquitination depending on cellular context highlighting the complexity of post-translational regulation [XREF_BIBR]."

reach
"As an example, USP26 physically interacts with AR and influences AR ubiquitination and transcriptional activation [XREF_BIBR]."

sparser
"Usp26 can directly bind and deubiquitinate AR, acting as a co-regulator of the AR by reversing AR activation and degradation by MDM2 ubiquitination depending on cellular context highlighting the complexity of post-translational regulation [ xref ]."

reach
"With mRNA and protein expression of USP26 confirmed in breast and thyroid cancer, the interaction of USP26 and AR may play a significant role in tumorigenesis."

reach
"With abnormal USP26 expression (both under and overexpression) identified in breast tissue, additional investigation is warranted given the known USP26 interaction with androgen receptor."
USP26 binds AR and nuclear receptor. 2 / 2
| 2

sparser
"USP26 binds to androgen receptor through three nuclear receptor interaction motifs, and knockdown of USP26 leads to decreased receptor activity in LnCaP and HEK293 cells (Dirac and Bernards, xref )."

sparser
"Usp26 binds to AR via nuclear receptor boxes, a hydrophobic motif (LXXLL) found in p160 coactivators."
AR binds USP26. 1 / 1
| 1

isi
"Moreover, the results from co-immunoprecipitation (CO-IP), immunofluorescence and western blot assays showed that the physiological process due to USP26 interacted with AR and influenced AR deubiquitination, thus upregulating the proteins CCND1 and SPATA46 - which are associated with cell cycle progression and spermatogenesis - as well as decreasing the expression of TP73."
USP26 binds TBCB and AR. 1 / 1
| 1

sparser
"Our data suggest that USP26 assembles with AR and other cofactors in subnuclear foci, and serves to counteract hormone-induced AR ubiquitination, thereby contributing to the regulation of AR transcriptional activity."
USP26 deubiquitinates AR.
1 | 1 4
USP26 deubiquitinates AR. 6 / 6
1 | 1 4

reach
"Taken together, the effects of AR deubiquitinated by USP26 could modulate sperm maturation and spermatogenesis through the androgen receptor signaling pathway."

reach
"Elevated cellular ROS levels might then inhibit USP26 activity to increase the ubiquitination of androgen receptor (AR) and AR splice variant 7 (ARv7) and their ubiquitin and proteasome dependent degradation, which contributed to the increase of Enz sensitivity."

trips
"USP26 deubiquitinates androgen receptor (AR) in the maintenance of sperm maturation and spermatogenesis through the androgen receptor signaling pathway."

"Our data suggest that USP26 assembles with AR and other cofactors in subnuclear foci, and serves to counteract hormone-induced AR ubiquitination, thereby contributing to the regulation of AR transcriptional activity."

reach
"USP26 can deubiquitylate AR in the presence of androgen, while USP10 and 2A-DUB both regulate AR transcriptional activity but do not alter protein stability."

reach
"USP26 deubiquitinates androgen receptor (AR) in the maintenance of sperm maturation and spermatogenesis through the androgen receptor signaling pathway."
USP26 ubiquitinates AR.
| 1
USP26 ubiquitinates AR. 1 / 1
| 1

reach
"Elevated cellular ROS levels might then inhibit USP26 activity to increase the ubiquitination of androgen receptor (AR) and AR splice variant 7 (ARv7) and their ubiquitin and proteasome dependent degradation, which contributed to the increase of Enz sensitivity."
USP26 decreases the amount of AR.
| 1
USP26 decreases the amount of AR. 1 / 1
| 1

reach
"To further dissect the molecular mechanism (s) how miR-367-3p increases AR expression, according to the screening strategy and bioinformatics analysis, we focused on the 2 miR-367-3p predicted targets, MDM2 and USP26, that were reported to modulate AR protein expression."
USP26 activates AR.
| 1
USP26 activates AR. 1 / 1
| 1

reach
"In LNCaP cells, Usp26 depletion by siRNA enhances AR dependent transcription, suggesting it acts on a factor that is negatively regulated by ubiquitination (Dirac and Bernards, 2010)."
USP26 affects CBX4
1 | 8 9
USP26 binds CBX4.
1 | 6 9
1 | 6

reach
"To determine whether USP26 interacts with the PRC1 components RING1A, CBX4, and CBX6 independent of each other, we generated RING1A, CBX4, or CBX6 knockout 293T cells using CRISPR and Cas9 technology."

reach
"ChIP-qPCR of the promoters of Sox2, Nanog, Oct4, and Cbx7 in Usp26 differentiated ESCs on day 6 revealed binding of USP26, CBX4, CBX6, H2A-ubi1, and H3K27me3 to the Sox2, Nanog, and Cbx7 promoters, suggesting that promoter binding of these components led to a decrease in pluripotency gene expression."

reach
"However, when USP26 was expressed with either Cbx4 or CBX6, significantly decreased promoter activities of Sox2, Nanog, and Cbx7 were observed, suggesting that USP26 interacts with Cbx4 or Cbx6 alone or both in Cbx7 promoter for CBX7 suppression."

reach
"USP26 binds to CBX4 and CBX6 in RING1A independent manner."

No evidence text available

reach
"Since the undifferentiated ESCs expressed CBX7 but no USP26, endogenous interactions between USP26 and CBX4 or CBX6, but not CBX7, were only observed in differentiating ESCs."

reach
"IP experiments showed binding of FLAG-USP26 and CBX4 or CBX6 in RING1A KO 293T cells, suggesting that USP26 and CBX4 or CBX6 interactions are RING1A independent."
USP26 binds CBX4 and CBX6. 5 / 5
| 5

sparser
"IP experiments showed binding of FLAG-USP26 and CBX4 or CBX6 in RING1A KO 293T cells (Fig.  xref ), suggesting that USP26 and CBX4 or CBX6 interactions are RING1A-independent."

sparser
"However, when USP26 was expressed with either Cbx4 or CBX6, significantly decreased promoter activities of Sox2, Nanog , and Cbx7 were observed (Supplementary Fig.  xref ), suggesting that USP26 interacts with Cbx4 or Cbx6 alone or both in Cbx7 promoter for CBX7 suppression."

sparser
"However, the interaction of USP26 with CBX4 and CBX6 was independent of RING1A. In differentiated cells, USP26 removed K48-linked Ub chains from CBX4 and CBX6, thus stabilizing the proteins and preventing their degradation."

sparser
"Since the undifferentiated ESCs expressed CBX7 but no USP26, endogenous interactions between USP26 and CBX4 or CBX6, but not CBX7, were only observed in differentiating ESCs (Fig.  xref )."

sparser
"Taken together, these results suggest that USP26 physically binds to components of the PRC1 complex, including RING1A, CBX4, and CBX6, and the interaction between USP26 and CBX4 or CBX6 is independent of the presence of RING1A."
RING1 binds USP26, CBX4, and CBX6. 2 / 2
| 2

sparser
"USP26 binds to CBX4 and CBX6 in RING1A-independent manner."

sparser
"However, in CBX4 or CBX6 knockout 293T cells, USP26 did not interact with RING1A, suggesting that USP26 and RING1A interactions are dependent on the presence of CBX4 or CBX6 (Fig.  xref )."
USP26 binds CBX4, CBX6, and PRC1. 1 / 1
| 1

sparser
"We further identified that USP26 directly bound to CBX4 and CBX6 proteins and other components of the PRC1.2 complex, including RING1A, PCGF2, and CBX7."
| 1

sparser
"To determine whether USP26 interacts with the PRC1 components RING1A, CBX4, and CBX6 independent of each other, we generated RING1A, CBX4, or CBX6 knockout 293T cells using CRISPR/Cas9 technology (Supplementary Fig. xref c, d)."
USP26 activates CBX4.
| 2
USP26 activates CBX4. 2 / 4
| 2

reach
"This result suggests that the increased Usp26 expression during ESC differentiation may lead to increased CBX4 and CBX6 protein expression not only by CBX4 and CBX6 protein stabilization but also indirectly by reversing the repression of their gene expression."

reach
"Taken together, these results provide molecular mechanisms of Usp26 mediated increased CBX4 and CBX6 protein stability for suppressing pluripotent gene expression."
AR affects USP26
3 | 9 7 1
3 | 9 4

sparser
"Our study also provides the evidence on the interactions between USP26 and AR in mouse testis, whereby pointing to a potential mechanism."

reach
"Given USP26 interaction with the androgen receptor, we conducted this study to characterize USP26 as a potential target affecting human testicular function and fertility and androgen dependent cancers."

sparser
"As an example, USP26 physically interacts with AR and influences AR ubiquitination and transcriptional activation [ xref ]."

reach
"Our study also provides the evidence on the interactions between USP26 and AR in mouse testis, whereby pointing to a potential mechanism."

reach
"USP26 binds to androgen receptor through three nuclear receptor interaction motifs, and knockdown of USP26 leads to decreased receptor activity in LnCaP and HEK293 cells."

reach
"Usp26 can directly bind and deubiquitinate AR, acting as a co-regulator of the AR by reversing AR activation and degradation by MDM2 ubiquitination depending on cellular context highlighting the complexity of post-translational regulation [XREF_BIBR]."

reach
"As an example, USP26 physically interacts with AR and influences AR ubiquitination and transcriptional activation [XREF_BIBR]."

sparser
"Usp26 can directly bind and deubiquitinate AR, acting as a co-regulator of the AR by reversing AR activation and degradation by MDM2 ubiquitination depending on cellular context highlighting the complexity of post-translational regulation [ xref ]."

reach
"With mRNA and protein expression of USP26 confirmed in breast and thyroid cancer, the interaction of USP26 and AR may play a significant role in tumorigenesis."

reach
"With abnormal USP26 expression (both under and overexpression) identified in breast tissue, additional investigation is warranted given the known USP26 interaction with androgen receptor."
USP26 binds AR and nuclear receptor. 2 / 2
| 2

sparser
"USP26 binds to androgen receptor through three nuclear receptor interaction motifs, and knockdown of USP26 leads to decreased receptor activity in LnCaP and HEK293 cells (Dirac and Bernards, xref )."

sparser
"Usp26 binds to AR via nuclear receptor boxes, a hydrophobic motif (LXXLL) found in p160 coactivators."
AR binds USP26. 1 / 1
| 1

isi
"Moreover, the results from co-immunoprecipitation (CO-IP), immunofluorescence and western blot assays showed that the physiological process due to USP26 interacted with AR and influenced AR deubiquitination, thus upregulating the proteins CCND1 and SPATA46 - which are associated with cell cycle progression and spermatogenesis - as well as decreasing the expression of TP73."
USP26 binds TBCB and AR. 1 / 1
| 1

sparser
"Our data suggest that USP26 assembles with AR and other cofactors in subnuclear foci, and serves to counteract hormone-induced AR ubiquitination, thereby contributing to the regulation of AR transcriptional activity."
USP26 affects CBX6
1 | 6 9
USP26 binds CBX6.
1 | 5 9
1 | 5

No evidence text available

reach
"To determine whether USP26 interacts with the PRC1 components RING1A, CBX4, and CBX6 independent of each other, we generated RING1A, CBX4, or CBX6 knockout 293T cells using CRISPR and Cas9 technology."

reach
"Since the undifferentiated ESCs expressed CBX7 but no USP26, endogenous interactions between USP26 and CBX4 or CBX6, but not CBX7, were only observed in differentiating ESCs."

reach
"However, when USP26 was expressed with either Cbx4 or CBX6, significantly decreased promoter activities of Sox2, Nanog, and Cbx7 were observed, suggesting that USP26 interacts with Cbx4 or Cbx6 alone or both in Cbx7 promoter for CBX7 suppression."

reach
"USP26 binds to CBX4 and CBX6 in RING1A independent manner."

reach
"ChIP-qPCR of the promoters of Sox2, Nanog, Oct4, and Cbx7 in Usp26 differentiated ESCs on day 6 revealed binding of USP26, CBX4, CBX6, H2A-ubi1, and H3K27me3 to the Sox2, Nanog, and Cbx7 promoters, suggesting that promoter binding of these components led to a decrease in pluripotency gene expression."
USP26 binds CBX4 and CBX6. 5 / 5
| 5

sparser
"IP experiments showed binding of FLAG-USP26 and CBX4 or CBX6 in RING1A KO 293T cells (Fig.  xref ), suggesting that USP26 and CBX4 or CBX6 interactions are RING1A-independent."

sparser
"However, when USP26 was expressed with either Cbx4 or CBX6, significantly decreased promoter activities of Sox2, Nanog , and Cbx7 were observed (Supplementary Fig.  xref ), suggesting that USP26 interacts with Cbx4 or Cbx6 alone or both in Cbx7 promoter for CBX7 suppression."

sparser
"However, the interaction of USP26 with CBX4 and CBX6 was independent of RING1A. In differentiated cells, USP26 removed K48-linked Ub chains from CBX4 and CBX6, thus stabilizing the proteins and preventing their degradation."

sparser
"Since the undifferentiated ESCs expressed CBX7 but no USP26, endogenous interactions between USP26 and CBX4 or CBX6, but not CBX7, were only observed in differentiating ESCs (Fig.  xref )."

sparser
"Taken together, these results suggest that USP26 physically binds to components of the PRC1 complex, including RING1A, CBX4, and CBX6, and the interaction between USP26 and CBX4 or CBX6 is independent of the presence of RING1A."
RING1 binds USP26, CBX4, and CBX6. 2 / 2
| 2

sparser
"USP26 binds to CBX4 and CBX6 in RING1A-independent manner."

sparser
"However, in CBX4 or CBX6 knockout 293T cells, USP26 did not interact with RING1A, suggesting that USP26 and RING1A interactions are dependent on the presence of CBX4 or CBX6 (Fig.  xref )."
USP26 binds CBX4, CBX6, and PRC1. 1 / 1
| 1

sparser
"We further identified that USP26 directly bound to CBX4 and CBX6 proteins and other components of the PRC1.2 complex, including RING1A, PCGF2, and CBX7."
| 1

sparser
"To determine whether USP26 interacts with the PRC1 components RING1A, CBX4, and CBX6 independent of each other, we generated RING1A, CBX4, or CBX6 knockout 293T cells using CRISPR/Cas9 technology (Supplementary Fig. xref c, d)."
USP26 activates CBX6.
| 1
USP26 activates CBX6. 1 / 3
| 1

reach
"Taken together, these results provide molecular mechanisms of Usp26 mediated increased CBX4 and CBX6 protein stability for suppressing pluripotent gene expression."
CBX4 affects USP26
1 | 6 9
1 | 6

reach
"To determine whether USP26 interacts with the PRC1 components RING1A, CBX4, and CBX6 independent of each other, we generated RING1A, CBX4, or CBX6 knockout 293T cells using CRISPR and Cas9 technology."

reach
"ChIP-qPCR of the promoters of Sox2, Nanog, Oct4, and Cbx7 in Usp26 differentiated ESCs on day 6 revealed binding of USP26, CBX4, CBX6, H2A-ubi1, and H3K27me3 to the Sox2, Nanog, and Cbx7 promoters, suggesting that promoter binding of these components led to a decrease in pluripotency gene expression."

reach
"However, when USP26 was expressed with either Cbx4 or CBX6, significantly decreased promoter activities of Sox2, Nanog, and Cbx7 were observed, suggesting that USP26 interacts with Cbx4 or Cbx6 alone or both in Cbx7 promoter for CBX7 suppression."

reach
"USP26 binds to CBX4 and CBX6 in RING1A independent manner."

No evidence text available

reach
"Since the undifferentiated ESCs expressed CBX7 but no USP26, endogenous interactions between USP26 and CBX4 or CBX6, but not CBX7, were only observed in differentiating ESCs."

reach
"IP experiments showed binding of FLAG-USP26 and CBX4 or CBX6 in RING1A KO 293T cells, suggesting that USP26 and CBX4 or CBX6 interactions are RING1A independent."
USP26 binds CBX4 and CBX6. 5 / 5
| 5

sparser
"IP experiments showed binding of FLAG-USP26 and CBX4 or CBX6 in RING1A KO 293T cells (Fig.  xref ), suggesting that USP26 and CBX4 or CBX6 interactions are RING1A-independent."

sparser
"However, when USP26 was expressed with either Cbx4 or CBX6, significantly decreased promoter activities of Sox2, Nanog , and Cbx7 were observed (Supplementary Fig.  xref ), suggesting that USP26 interacts with Cbx4 or Cbx6 alone or both in Cbx7 promoter for CBX7 suppression."

sparser
"However, the interaction of USP26 with CBX4 and CBX6 was independent of RING1A. In differentiated cells, USP26 removed K48-linked Ub chains from CBX4 and CBX6, thus stabilizing the proteins and preventing their degradation."

sparser
"Since the undifferentiated ESCs expressed CBX7 but no USP26, endogenous interactions between USP26 and CBX4 or CBX6, but not CBX7, were only observed in differentiating ESCs (Fig.  xref )."

sparser
"Taken together, these results suggest that USP26 physically binds to components of the PRC1 complex, including RING1A, CBX4, and CBX6, and the interaction between USP26 and CBX4 or CBX6 is independent of the presence of RING1A."
RING1 binds USP26, CBX4, and CBX6. 2 / 2
| 2

sparser
"USP26 binds to CBX4 and CBX6 in RING1A-independent manner."

sparser
"However, in CBX4 or CBX6 knockout 293T cells, USP26 did not interact with RING1A, suggesting that USP26 and RING1A interactions are dependent on the presence of CBX4 or CBX6 (Fig.  xref )."
USP26 binds CBX4, CBX6, and PRC1. 1 / 1
| 1

sparser
"We further identified that USP26 directly bound to CBX4 and CBX6 proteins and other components of the PRC1.2 complex, including RING1A, PCGF2, and CBX7."
| 1

sparser
"To determine whether USP26 interacts with the PRC1 components RING1A, CBX4, and CBX6 independent of each other, we generated RING1A, CBX4, or CBX6 knockout 293T cells using CRISPR/Cas9 technology (Supplementary Fig. xref c, d)."
USP26 affects SMAD7
1 | 12 3
USP26 binds SMAD7.
| 2 3
| 2 2

reach
"TGF-beta treatment enhanced the binding of USP26 to SMAD7 but not SMAD6 (Fig XREF_FIG C and D)."

sparser
"TGF‐β treatment enhanced the binding of USP26 to SMAD7 but not SMAD6 (Fig  xref C and D)."

reach
"Therefore, in light of our findings that both USP26 and SMAD7 are upregulated following stimulation with TGF-beta, we speculated that USP26 might form a complex with SMAD7 following TGF-beta exposure leading to USP26 mediated deubiquitination."

sparser
"Therefore, in light of our findings that both USP26 and SMAD7 are upregulated following stimulation with TGF‐β, we speculated that USP26 might form a complex with SMAD7 following TGF‐β exposure leading to USP26‐mediated deubiquitination."
| 1

sparser
"These data suggest that USP26 can form a stable complex in vivo with SMAD3, SMAD6, and SMAD7."
USP26 deubiquitinates SMAD7.
1 | 2
USP26 deubiquitinates SMAD7. 3 / 3
1 | 2

"USP26 regulates TGF-b signaling by deubiquitinating and stabilizing SMAD7."

reach
"USP26 deubiquitinates SMAD7."

reach
"Examples include ubiquitin specific protease 10 (USP10), which can act on SMAD4 to make it deubiquitinated and stable, further promoting TGF-beta signaling [XREF_BIBR], and USP26, which promotes SMAD7 deubiquitination, thereby amplifying the inhibitory effect of SMAD7 and strengthening the inhibition of the TGF-beta signaling pathway [XREF_BIBR]."
| PMC
USP26 activates SMAD7.
| 3
USP26 activates SMAD7. 3 / 3
| 3

reach
"Conversely, knockdown of USP26 rapidly degrades SMAD7 resulting in TGF-beta receptor stabilization and enhanced levels of p-SMAD2."

reach
"In contrast, loss of USP26 rapidly degrades SMAD7 stabilizing the TGF-beta receptors leading to enhanced TGF-beta activity as observed by increased levels of phosphorylated SMAD2."

reach
"As loss of USP26 degrades SMAD7 leading to the stabilization of TbetaR, we evaluated the role of TbetaR and SMAD7 on clinical outcome."
USP26 inhibits SMAD7.
| 2
USP26 inhibits SMAD7. 2 / 2
| 2

reach
"As observed with USP26 knockdown, ectopic expression of the DUB dead mutant recapitulated the augmentation of SMAD7 induction by TGF-beta whereas wild-type USP26 significantly diminished the induction of SMAD7 in the presence of TGF-beta (Fig XREF_FIG F)."

reach
"Therefore, we analyzed whether loss of USP26 increases the levels of SMAD7 Lys48 or Lys63 incorporated ubiquitin chains."
USP26 increases the amount of SMAD7.
| 2
Modified mutated USP26 increases the amount of SMAD7. 1 / 1
| 1

reach
"Overexpression of USP26, but not the catalytically inactive mutant USP26 C/S, markedly increased the expression of SMAD7 (Fig XREF_FIG A)."
Modified USP26 increases the amount of SMAD7. 1 / 1
| 1

reach
"Overexpression of USP26, but not the catalytically inactive mutant USP26 C/S, markedly increased the expression of SMAD7 (Fig XREF_FIG A)."
USP26 decreases the amount of SMAD7.
| 1
USP26 decreases the amount of SMAD7. 1 / 1
| 1

reach
"In addition, depletion of USP26 significantly reduced the expression of SMAD7 in both the MDA-MB-231 and U373 cell lines (Fig XREF_FIG F and G)."
CBX6 affects USP26
1 | 5 9
1 | 5

No evidence text available

reach
"To determine whether USP26 interacts with the PRC1 components RING1A, CBX4, and CBX6 independent of each other, we generated RING1A, CBX4, or CBX6 knockout 293T cells using CRISPR and Cas9 technology."

reach
"Since the undifferentiated ESCs expressed CBX7 but no USP26, endogenous interactions between USP26 and CBX4 or CBX6, but not CBX7, were only observed in differentiating ESCs."

reach
"However, when USP26 was expressed with either Cbx4 or CBX6, significantly decreased promoter activities of Sox2, Nanog, and Cbx7 were observed, suggesting that USP26 interacts with Cbx4 or Cbx6 alone or both in Cbx7 promoter for CBX7 suppression."

reach
"USP26 binds to CBX4 and CBX6 in RING1A independent manner."

reach
"ChIP-qPCR of the promoters of Sox2, Nanog, Oct4, and Cbx7 in Usp26 differentiated ESCs on day 6 revealed binding of USP26, CBX4, CBX6, H2A-ubi1, and H3K27me3 to the Sox2, Nanog, and Cbx7 promoters, suggesting that promoter binding of these components led to a decrease in pluripotency gene expression."
USP26 binds CBX4 and CBX6. 5 / 5
| 5

sparser
"IP experiments showed binding of FLAG-USP26 and CBX4 or CBX6 in RING1A KO 293T cells (Fig.  xref ), suggesting that USP26 and CBX4 or CBX6 interactions are RING1A-independent."

sparser
"However, when USP26 was expressed with either Cbx4 or CBX6, significantly decreased promoter activities of Sox2, Nanog , and Cbx7 were observed (Supplementary Fig.  xref ), suggesting that USP26 interacts with Cbx4 or Cbx6 alone or both in Cbx7 promoter for CBX7 suppression."

sparser
"However, the interaction of USP26 with CBX4 and CBX6 was independent of RING1A. In differentiated cells, USP26 removed K48-linked Ub chains from CBX4 and CBX6, thus stabilizing the proteins and preventing their degradation."

sparser
"Since the undifferentiated ESCs expressed CBX7 but no USP26, endogenous interactions between USP26 and CBX4 or CBX6, but not CBX7, were only observed in differentiating ESCs (Fig.  xref )."

sparser
"Taken together, these results suggest that USP26 physically binds to components of the PRC1 complex, including RING1A, CBX4, and CBX6, and the interaction between USP26 and CBX4 or CBX6 is independent of the presence of RING1A."
RING1 binds USP26, CBX4, and CBX6. 2 / 2
| 2

sparser
"USP26 binds to CBX4 and CBX6 in RING1A-independent manner."

sparser
"However, in CBX4 or CBX6 knockout 293T cells, USP26 did not interact with RING1A, suggesting that USP26 and RING1A interactions are dependent on the presence of CBX4 or CBX6 (Fig.  xref )."
USP26 binds CBX4, CBX6, and PRC1. 1 / 1
| 1

sparser
"We further identified that USP26 directly bound to CBX4 and CBX6 proteins and other components of the PRC1.2 complex, including RING1A, PCGF2, and CBX7."
| 1

sparser
"To determine whether USP26 interacts with the PRC1 components RING1A, CBX4, and CBX6 independent of each other, we generated RING1A, CBX4, or CBX6 knockout 293T cells using CRISPR/Cas9 technology (Supplementary Fig. xref c, d)."
USP26 affects MDM2
1 1 | 2 5 2
USP26 binds MDM2.
1 | 1 3 2
1 | 1 3 2

reach
"We found that USP26 binds to Mdm2 through its coiled coiled C-terminal domain."

sparser
"We found that USP26 binds to Mdm2 through its coiled-coiled C-terminal domain."

No evidence text available

sparser
"The interaction between USP26 and Mdm2, and the subsequent deubiquitination of Mdm2, serves, most probably to regulate Mdm2."

reach
"Future therapeutic modalities that interfere with the association between USP26 and Mdm2 will be used to destabilize the ligase in malignancies where it is upregulated."

trips
"We found that USP26 binds to Mdm2 through its coiled-coiled C-terminal domain."

reach
"The interaction between USP26 and Mdm2, and the subsequent deubiquitination of Mdm2, serves, most probably to regulate Mdm2."
USP26 deubiquitinates MDM2.
1 | 1 2
USP26 deubiquitinates MDM2. 4 / 4
1 | 1 2

reach
"USP26 also deubiquitinates and stabilizes Mdm2 [70] thus underlining the complex regulation of AR transcriptional activity."

trips
"USP26 deubiquitinates Mdm2 and stabilizes it."

reach
"USP26 deubiquitinates Mdm2 and stabilizes it."
USP26 affects PRC1
1 | 1 4
USP26 binds PRC1.
| 4
| 2

sparser
"Taken together, these results suggest that USP26 physically binds to components of the PRC1 complex, including RING1A, CBX4, and CBX6, and the interaction between USP26 and CBX4 or CBX6 is independent of the presence of RING1A."

sparser
"To understand how Usp26 expression is regulated, we assessed the promoter-binding activity of Usp26 by PRC1 subunits using the ChIP–qPCR assay during RA-induced ESC differentiation, and observed that PRC1 components PCGF2 and Cbx7 occupation as well as H2A-ubi1 modifications decreased significantly in Usp26 promoter (Supplementary Fig. xref d), thus indicating that CBX7 containing PRC1 complex is a potential repressor of Usp26 ."
USP26 binds CBX4, CBX6, and PRC1. 1 / 1
| 1

sparser
"We further identified that USP26 directly bound to CBX4 and CBX6 proteins and other components of the PRC1.2 complex, including RING1A, PCGF2, and CBX7."
| 1

sparser
"To determine whether USP26 interacts with the PRC1 components RING1A, CBX4, and CBX6 independent of each other, we generated RING1A, CBX4, or CBX6 knockout 293T cells using CRISPR/Cas9 technology (Supplementary Fig. xref c, d)."
USP26 deubiquitinates PRC1.
1 | 1
USP26 deubiquitinates PRC1. 2 / 2
1 | 1

reach
"In some cancer types, PRC1 can be deubiquitinated by USP7, USP11 and USP26 [XREF_BIBR, XREF_BIBR]."
CBX6 affects CBX4
| 9
USP26 binds CBX4 and CBX6. 5 / 5
| 5

sparser
"IP experiments showed binding of FLAG-USP26 and CBX4 or CBX6 in RING1A KO 293T cells (Fig.  xref ), suggesting that USP26 and CBX4 or CBX6 interactions are RING1A-independent."

sparser
"However, when USP26 was expressed with either Cbx4 or CBX6, significantly decreased promoter activities of Sox2, Nanog , and Cbx7 were observed (Supplementary Fig.  xref ), suggesting that USP26 interacts with Cbx4 or Cbx6 alone or both in Cbx7 promoter for CBX7 suppression."

sparser
"However, the interaction of USP26 with CBX4 and CBX6 was independent of RING1A. In differentiated cells, USP26 removed K48-linked Ub chains from CBX4 and CBX6, thus stabilizing the proteins and preventing their degradation."

sparser
"Since the undifferentiated ESCs expressed CBX7 but no USP26, endogenous interactions between USP26 and CBX4 or CBX6, but not CBX7, were only observed in differentiating ESCs (Fig.  xref )."

sparser
"Taken together, these results suggest that USP26 physically binds to components of the PRC1 complex, including RING1A, CBX4, and CBX6, and the interaction between USP26 and CBX4 or CBX6 is independent of the presence of RING1A."
RING1 binds USP26, CBX4, and CBX6. 2 / 2
| 2

sparser
"USP26 binds to CBX4 and CBX6 in RING1A-independent manner."

sparser
"However, in CBX4 or CBX6 knockout 293T cells, USP26 did not interact with RING1A, suggesting that USP26 and RING1A interactions are dependent on the presence of CBX4 or CBX6 (Fig.  xref )."
USP26 binds CBX4, CBX6, and PRC1. 1 / 1
| 1

sparser
"We further identified that USP26 directly bound to CBX4 and CBX6 proteins and other components of the PRC1.2 complex, including RING1A, PCGF2, and CBX7."
| 1

sparser
"To determine whether USP26 interacts with the PRC1 components RING1A, CBX4, and CBX6 independent of each other, we generated RING1A, CBX4, or CBX6 knockout 293T cells using CRISPR/Cas9 technology (Supplementary Fig. xref c, d)."
PRC1 affects USP26
| 4
| 2

sparser
"Taken together, these results suggest that USP26 physically binds to components of the PRC1 complex, including RING1A, CBX4, and CBX6, and the interaction between USP26 and CBX4 or CBX6 is independent of the presence of RING1A."

sparser
"To understand how Usp26 expression is regulated, we assessed the promoter-binding activity of Usp26 by PRC1 subunits using the ChIP–qPCR assay during RA-induced ESC differentiation, and observed that PRC1 components PCGF2 and Cbx7 occupation as well as H2A-ubi1 modifications decreased significantly in Usp26 promoter (Supplementary Fig. xref d), thus indicating that CBX7 containing PRC1 complex is a potential repressor of Usp26 ."
USP26 binds CBX4, CBX6, and PRC1. 1 / 1
| 1

sparser
"We further identified that USP26 directly bound to CBX4 and CBX6 proteins and other components of the PRC1.2 complex, including RING1A, PCGF2, and CBX7."
| 1

sparser
"To determine whether USP26 interacts with the PRC1 components RING1A, CBX4, and CBX6 independent of each other, we generated RING1A, CBX4, or CBX6 knockout 293T cells using CRISPR/Cas9 technology (Supplementary Fig. xref c, d)."
MDM2 affects USP26
1 | 1 3 2
1 | 1 3 2

reach
"We found that USP26 binds to Mdm2 through its coiled coiled C-terminal domain."

sparser
"We found that USP26 binds to Mdm2 through its coiled-coiled C-terminal domain."

No evidence text available

sparser
"The interaction between USP26 and Mdm2, and the subsequent deubiquitination of Mdm2, serves, most probably to regulate Mdm2."

reach
"Future therapeutic modalities that interfere with the association between USP26 and Mdm2 will be used to destabilize the ligase in malignancies where it is upregulated."

trips
"We found that USP26 binds to Mdm2 through its coiled-coiled C-terminal domain."

reach
"The interaction between USP26 and Mdm2, and the subsequent deubiquitination of Mdm2, serves, most probably to regulate Mdm2."

reach
"USP26 expression promotes ESC differentiation."

reach
"Conversely, lesser extent of CBX7 protein binding to the promoters of Cbx4 and Cbx6 is observed during Usp26 mediated ESC differentiation and RA induced ESC differentiation."

reach
"Correlative with the Oct4 gene expression data, occupancy at the Oct4 promoter by USP26, CBX4, CBX6, and H2A-ubi1 was low even during USP26 mediated ESC differentiation."

reach
"Furthermore, infection of ESCs with GFP tagged mouse (m) USP26 led to ESC differentiation in an RA independent manner."

reach
"Using screening of shRNA specific for the DUB subfamily USPs, we identified a novel cellular reprogramming repressor gene Usp26, which blocked somatic cell reprogramming into iPSCs and promoted differentiation by stabilizing PRC1 complexes."

reach
"We also found significant decrease in the gene expression of Cbx7, a Polycomb protein, upon Usp26 induced ESC differentiation."

reach
"These results suggest that USP26 expression promotes ESC differentiation."
USP26 affects TGFB
| 7
USP26 inhibits TGFB.
| 5
USP26 inhibits TGFB. 5 / 5
| 5

reach
"These results suggest that USP26 may potentially act in conjunction with SMAD7 and SMURF2 to downregulate canonical TGF-beta signaling."

reach
"Taken together, these findings confirmed our hypothesis that loss of USP26 enhances TGF-beta signaling and confers poor prognosis in glioblastoma patients."

reach
"Taken together, these data indicate that USP26 inhibits TGF-beta pathway activity by limiting Lys48 ubiquitin mediated degradation of SMAD7 consequently resulting in enhanced ubiquitylation and degradation of the TbetaR complex."

reach
"As knockdown of USP26 potently activated the TGF-beta pathway in breast cancer, we investigated whether depletion of USP26 in the TGF-beta-responsive metastatic cell line MDA-MB-231 enhanced cellular motility and invasion."

reach
"In line with our other models, knockdown of USP26 in this patient derived cell line enhanced TGF-beta activity as evidenced by increased levels of phospho-SMAD2 and increased expression of TGF-beta target genes CTGF, LIF, and SMAD7 (Fig XREF_FIG D and E)."
USP26 phosphorylates TGFB.
| 1
USP26 leads to the phosphorylation of TGFB. 1 / 1
| 1

reach
"As USP26 appears to mediate SMAD2/3 phosphorylation and downstream TGF-beta activity, we reasoned that USP26 may function through the canonical TGF-beta pathway."
USP26 activates TGFB.
| 1
USP26 activates TGFB. 1 / 1
| 1

reach
"USP26 depletion enhances TGF-beta activity and TGF-beta biological responses in breast cancer and glioma."
USP26 affects SOX2
| 5
USP26 binds SOX2.
| 2
| 2

reach
"ChIP-qPCR of the promoters of Sox2, Nanog, Oct4, and Cbx7 in Usp26 differentiated ESCs on day 6 revealed binding of USP26, CBX4, CBX6, H2A-ubi1, and H3K27me3 to the Sox2, Nanog, and Cbx7 promoters, suggesting that promoter binding of these components led to a decrease in pluripotency gene expression."

reach
"Similarly, during RA induced ESC differentiation, USP26, CBX4, CBX6, H2A-ubi1, and H3K27me3 also bound to the Sox2, Nanog, and Cbx7 promoters."
USP26 inhibits SOX2.
| 1
USP26 inhibits SOX2. 1 / 2
| 1

reach
"By contrast, knockdown of Usp26 enhances the efficiency of reprogramming by reactivating Sox2 and Nanog through degradation of CBX4 and CBX6."
USP26 decreases the amount of SOX2.
| 2
Modified USP26 decreases the amount of SOX2. 1 / 1
| 1

reach
"Overexpression of Usp26 in ESCs led to decreased mRNA levels of Sox2, Nanog, and Oct4 compared with control cells."
USP26 decreases the amount of SOX2. 1 / 1
| 1

reach
"Our results showed that after Usp26 knockdown during Dox induced OSKM mediated MEF reprogramming led to increased mRNA levels of Sox2 and Nanog, but not Oct4, compared with control."
USP26 affects NANOG
| 5
USP26 binds NANOG.
| 2
| 2

reach
"ChIP-qPCR of the promoters of Sox2, Nanog, Oct4, and Cbx7 in Usp26 differentiated ESCs on day 6 revealed binding of USP26, CBX4, CBX6, H2A-ubi1, and H3K27me3 to the Sox2, Nanog, and Cbx7 promoters, suggesting that promoter binding of these components led to a decrease in pluripotency gene expression."

reach
"Similarly, during RA induced ESC differentiation, USP26, CBX4, CBX6, H2A-ubi1, and H3K27me3 also bound to the Sox2, Nanog, and Cbx7 promoters."
USP26 inhibits NANOG.
| 1
USP26 inhibits NANOG. 1 / 2
| 1

reach
"By contrast, knockdown of Usp26 enhances the efficiency of reprogramming by reactivating Sox2 and Nanog through degradation of CBX4 and CBX6."
USP26 decreases the amount of NANOG.
| 1
USP26 decreases the amount of NANOG. 1 / 1
| 1

reach
"Our results showed that after Usp26 knockdown during Dox induced OSKM mediated MEF reprogramming led to increased mRNA levels of Sox2 and Nanog, but not Oct4, compared with control."
USP26 activates NANOG.
| 1
Modified USP26 activates NANOG. 1 / 1
| 1

reach
"Overexpression of Usp26 in ESCs led to decreased mRNA levels of Sox2, Nanog, and Oct4 compared with control cells."

reach
"Given that USP26 and USP37 are able to reverse RNF8/168 mediated ubiquitylation and promote HR, these enzymes would be ideal candidates to facilitate the repositioning of ubiquitylated substrates away from the DSB."

reach
"A striking conclusion from our study is that both over-expression as well as knockdown of USP26 or USP37 impairs HR."

reach
"Loss of USP26 or USP37 impairs HR by counteracting RAP80 dependent sequestration of BRCA1."

reach
"Loss of USP26 and USP37 function markedly impairs the assembly of PALB2, RAD51 and efficient HR."

reach
"Depletion of USP26 or USP37 disrupts the execution of HR and this effect is alleviated by the simultaneous depletion of RAP80."

reach
"We reasoned that USP26 and USP37 may antagonize the RAP80 dependent sequestration of BRCA1 by removing RNF8 and RNF168 mediated ubiquitylation and thereby promote HR."
USP26 affects RING1
| 4
| 1

sparser
"However, in CBX4 or CBX6 knockout 293T cells, USP26 did not interact with RING1A, suggesting that USP26 and RING1A interactions are dependent on the presence of CBX4 or CBX6 (Fig.  xref )."
RING1 binds USP26, CBX4, and CBX6. 2 / 2
| 2

sparser
"USP26 binds to CBX4 and CBX6 in RING1A-independent manner."

sparser
"However, in CBX4 or CBX6 knockout 293T cells, USP26 did not interact with RING1A, suggesting that USP26 and RING1A interactions are dependent on the presence of CBX4 or CBX6 (Fig.  xref )."
| 1

sparser
"To determine whether USP26 interacts with the PRC1 components RING1A, CBX4, and CBX6 independent of each other, we generated RING1A, CBX4, or CBX6 knockout 293T cells using CRISPR/Cas9 technology (Supplementary Fig. xref c, d)."
USP26 affects BRCA1
| 6
USP26 inhibits BRCA1.
| 3
USP26 inhibits BRCA1. 3 / 3
| 3

reach
"Together, these results suggest that USP26 and USP37 promote the BRCA1 dependent loading of PALB2 and RAD51 by counteracting the repressive impact of RAP80 dependent BRCA1 sequestration and RAP80 dependent inhibition of end-resection during HR."

reach
"We reasoned that USP26 and USP37 may antagonize the RAP80 dependent sequestration of BRCA1 by removing RNF8 and RNF168 mediated ubiquitylation and thereby promote HR."

reach
"In agreement with this hypothesis, we found that USP26 or USP37 depletion inhibits the efficient association of BRCA1 with PALB2, a unique subunit of the BRCC complex."
USP26 activates BRCA1.
| 3
USP26 activates BRCA1. 3 / 3
| 3

reach
"Loss of USP26 or USP37 impairs HR by counteracting RAP80 dependent sequestration of BRCA1."

reach
"On the other hand, we also found that USP26 and USP37 promote the efficient association of BRCA1 with PALB2."

reach
"Together, these results suggest that USP26 and USP37 promote the BRCA1 dependent loading of PALB2 and RAD51 by counteracting the repressive impact of RAP80 dependent BRCA1 sequestration and RAP80 dependent inhibition of end-resection during HR."
TGFB affects USP26
| 6
TGFB increases the amount of USP26.
| 5
TGFB increases the amount of USP26. 5 / 5
| 5

reach
"Further experimentation will be required to elucidate the precise mechanism of how TGF-beta enhances USP26 expression."

reach
"In all cell lines tested, TGF-beta enhanced the mRNA expression levels of both USP26 and SMAD7."

reach
"We demonstrate that TGF-beta rapidly enhances the expression of USP26 and reinforces SMAD7 stability by limiting the ubiquitin mediated turnover of SMAD7."

reach
"TGFbeta enhances the expression of USP26 and reinforces SMAD7 stability by limiting the ubiquitin mediated turnover of SMAD7."

reach
"Our data suggest that as part of a negative feedback loop, TGF-beta enhances the expression of not only SMAD7 but also USP26, which acts to deubiquitinate and stabilize SMAD7."
TGFB activates USP26.
| 1
TGFB activates USP26. 1 / 1
| 1

reach
"To verify whether USP26 induction by TGF-beta is prevalent in other cell types, we analyzed the induction of USP26 and SMAD7 at early time points in the TGF-beta-responsive breast cancer cell lines MCF7, MDA-MB-231, T47D, and CAL51 and glioma cell lines U373, PCTC, and A172 (Fig XREF_FIG A-G)."
RING1 affects USP26
| 4
| 1

sparser
"However, in CBX4 or CBX6 knockout 293T cells, USP26 did not interact with RING1A, suggesting that USP26 and RING1A interactions are dependent on the presence of CBX4 or CBX6 (Fig.  xref )."
RING1 binds USP26, CBX4, and CBX6. 2 / 2
| 2

sparser
"USP26 binds to CBX4 and CBX6 in RING1A-independent manner."

sparser
"However, in CBX4 or CBX6 knockout 293T cells, USP26 did not interact with RING1A, suggesting that USP26 and RING1A interactions are dependent on the presence of CBX4 or CBX6 (Fig.  xref )."
| 1

sparser
"To determine whether USP26 interacts with the PRC1 components RING1A, CBX4, and CBX6 independent of each other, we generated RING1A, CBX4, or CBX6 knockout 293T cells using CRISPR/Cas9 technology (Supplementary Fig. xref c, d)."
SMAD7 affects USP26
| 2 3
| 2 2

reach
"TGF-beta treatment enhanced the binding of USP26 to SMAD7 but not SMAD6 (Fig XREF_FIG C and D)."

sparser
"TGF‐β treatment enhanced the binding of USP26 to SMAD7 but not SMAD6 (Fig  xref C and D)."

reach
"Therefore, in light of our findings that both USP26 and SMAD7 are upregulated following stimulation with TGF-beta, we speculated that USP26 might form a complex with SMAD7 following TGF-beta exposure leading to USP26 mediated deubiquitination."

sparser
"Therefore, in light of our findings that both USP26 and SMAD7 are upregulated following stimulation with TGF‐β, we speculated that USP26 might form a complex with SMAD7 following TGF‐β exposure leading to USP26‐mediated deubiquitination."
| 1

sparser
"These data suggest that USP26 can form a stable complex in vivo with SMAD3, SMAD6, and SMAD7."
CBX4 affects CBX6, and USP26
| 5
USP26 binds CBX4 and CBX6. 5 / 5
| 5

sparser
"IP experiments showed binding of FLAG-USP26 and CBX4 or CBX6 in RING1A KO 293T cells (Fig.  xref ), suggesting that USP26 and CBX4 or CBX6 interactions are RING1A-independent."

sparser
"However, when USP26 was expressed with either Cbx4 or CBX6, significantly decreased promoter activities of Sox2, Nanog , and Cbx7 were observed (Supplementary Fig.  xref ), suggesting that USP26 interacts with Cbx4 or Cbx6 alone or both in Cbx7 promoter for CBX7 suppression."

sparser
"However, the interaction of USP26 with CBX4 and CBX6 was independent of RING1A. In differentiated cells, USP26 removed K48-linked Ub chains from CBX4 and CBX6, thus stabilizing the proteins and preventing their degradation."

sparser
"Since the undifferentiated ESCs expressed CBX7 but no USP26, endogenous interactions between USP26 and CBX4 or CBX6, but not CBX7, were only observed in differentiating ESCs (Fig.  xref )."

sparser
"Taken together, these results suggest that USP26 physically binds to components of the PRC1 complex, including RING1A, CBX4, and CBX6, and the interaction between USP26 and CBX4 or CBX6 is independent of the presence of RING1A."
USP26 affects UIMC1
| 4
USP26 inhibits UIMC1.
| 2
USP26 inhibits UIMC1. 2 / 2
| 2

reach
"We reasoned that USP26 and USP37 may antagonize the RAP80 dependent sequestration of BRCA1 by removing RNF8 and RNF168 mediated ubiquitylation and thereby promote HR."

reach
"Together, these results suggest that USP26 and USP37 promote the BRCA1 dependent loading of PALB2 and RAD51 by counteracting the repressive impact of RAP80 dependent BRCA1 sequestration and RAP80 dependent inhibition of end-resection during HR."
USP26 activates UIMC1.
| 2
USP26 activates UIMC1. 2 / 2
| 2

reach
"Loss of USP26 or USP37 impairs HR by counteracting RAP80 dependent sequestration of BRCA1."

reach
"Together, this model may explain how USP26 and USP37 limit the repressive impact of RAP80 on HR."
USP26 affects E7
4 |
2 |

No evidence text available

No evidence text available
1 |

No evidence text available
1 |

No evidence text available
USP26 affects CBX7
| 2
USP26 binds CBX7.
| 1
| 1

reach
"ChIP-qPCR of the promoters of Sox2, Nanog, Oct4, and Cbx7 in Usp26 differentiated ESCs on day 6 revealed binding of USP26, CBX4, CBX6, H2A-ubi1, and H3K27me3 to the Sox2, Nanog, and Cbx7 promoters, suggesting that promoter binding of these components led to a decrease in pluripotency gene expression."
USP26 decreases the amount of CBX7.
| 1
USP26 decreases the amount of CBX7. 1 / 1
| 1

reach
"Our results demonstrate that USP26 can diminish the expression of the Cbx7 gene and inhibit cellular reprogramming."
SOX2 affects USP26
| 3
SOX2 binds USP26.
| 2
| 2

reach
"ChIP-qPCR of the promoters of Sox2, Nanog, Oct4, and Cbx7 in Usp26 differentiated ESCs on day 6 revealed binding of USP26, CBX4, CBX6, H2A-ubi1, and H3K27me3 to the Sox2, Nanog, and Cbx7 promoters, suggesting that promoter binding of these components led to a decrease in pluripotency gene expression."

reach
"Similarly, during RA induced ESC differentiation, USP26, CBX4, CBX6, H2A-ubi1, and H3K27me3 also bound to the Sox2, Nanog, and Cbx7 promoters."
SOX2 activates USP26.
| 1
SOX2 activates USP26. 1 / 1
| 1

reach
"Sox2 and Nanog mRNA levels, but not Oct4, dramatically increased in Cbx4 or Cbx6 knockdown cells, similar to the increased Sox2 and Nanog mRNA levels in Usp26 knockdown cells."
NANOG affects USP26
| 3
NANOG binds USP26.
| 2
| 2

reach
"ChIP-qPCR of the promoters of Sox2, Nanog, Oct4, and Cbx7 in Usp26 differentiated ESCs on day 6 revealed binding of USP26, CBX4, CBX6, H2A-ubi1, and H3K27me3 to the Sox2, Nanog, and Cbx7 promoters, suggesting that promoter binding of these components led to a decrease in pluripotency gene expression."

reach
"Similarly, during RA induced ESC differentiation, USP26, CBX4, CBX6, H2A-ubi1, and H3K27me3 also bound to the Sox2, Nanog, and Cbx7 promoters."
NANOG activates USP26.
| 1
NANOG activates USP26. 1 / 1
| 1

reach
"Sox2 and Nanog mRNA levels, but not Oct4, dramatically increased in Cbx4 or Cbx6 knockdown cells, similar to the increased Sox2 and Nanog mRNA levels in Usp26 knockdown cells."
E7 affects USP26
4 |
2 |

No evidence text available

No evidence text available
1 |

No evidence text available
1 |

No evidence text available
CBX7 affects USP26
| 2
CBX7 binds USP26.
| 1
| 1

reach
"ChIP-qPCR of the promoters of Sox2, Nanog, Oct4, and Cbx7 in Usp26 differentiated ESCs on day 6 revealed binding of USP26, CBX4, CBX6, H2A-ubi1, and H3K27me3 to the Sox2, Nanog, and Cbx7 promoters, suggesting that promoter binding of these components led to a decrease in pluripotency gene expression."
CBX7 inhibits USP26.
| 1
CBX7 inhibits USP26. 1 / 1
| 1

reach
"To understand how Usp26 expression is regulated, we assessed the promoter binding activity of Usp26 by PRC1 subunits using the ChIP-qPCR assay during RA induced ESC differentiation, and observed that PRC1 components PCGF2 and Cbx7 occupation as well as H2A-ubi1 modifications decreased significantly in Usp26 promoter, thus indicating that CBX7 containing PRC1 complex is a potential repressor of Usp26."

reach
"The increased ROS levels generated by ABT263 can inhibit USP26 activity to ubiquitinate AR/ARv7, and further sensitize PCa cells to Enz treatment."

reach
"In our current study, we screened several AR related USPs, including USP7, USP12, USP14, USP22, and USP26 and found that the USP26 activity was repressed by the induced cellular ROS, which is responsible for AR/ARv7 protein degradation and Enz sensitivity increase by ABT263."

reach
"An in vitro DUB assay was applied to further detect USP26 activity, and results revealed that ABT263 could significantly decrease USP26 activity, which could be reversed by inhibition of cellular ROS (XREF_FIG H-J)."
USP26 affects SMAD3
| 1 2
| 1 1

sparser
"As part of this study, we demonstrate that USP26 binds to SMAD3, suggesting that USP26 may also regulate the TGF‐β pathway by enhancing androgen receptor‐mediated repression of SMAD3 transcriptional targets."

reach
"As part of this study, we demonstrate that USP26 binds to SMAD3, suggesting that USP26 may also regulate the TGF-beta pathway by enhancing androgen receptor mediated repression of SMAD3 transcriptional targets."
| 1

sparser
"These data suggest that USP26 can form a stable complex in vivo with SMAD3, SMAD6, and SMAD7."
USP26 affects POU5F1
| 3
USP26 activates POU5F1.
| 2
Modified USP26 activates POU5F1. 1 / 1
| 1