IndraLab

Statements


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"Interestingly, the indolactam specific TleC and MpnD both accepted different chain lengths (from C 5 to C 25) of prenyl pyrophosphate in vitro."

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"In contrast to typical indole PTs that exhibit broad substrate specificities towards the prenyl acceptors but with strict specificities towards the prenyl donors XREF_BIBR XREF_BIBR XREF_BIBR XREF_BIBR, it is remarkable that the indolactam specific TleC and MpnD exhibited unusually relaxed substrate specificities towards the prenyl donors and accepted different chain lengths (from C 5 to C 25) of prenyl pyrophosphate in vitro."

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"Interestingly, both TleC and MpnD also accepted farnesyl pyrophosphate (FPP) (C 15), geranylgeranyl pyrophosphate (GGPP) (C 20) and geranylfarnesyl pyrophosphate (GFPP) (C 25) as prenyl donors, to produce 5, 6 and 7, respectively (XREF_FIG and XREF_SUPPLEMENTARY)."
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Valproic acid methylates MPND.
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No evidence text available
Valproic acid decreases the amount of MPND.
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Valproic acid decreases the amount of MPND. 1 / 1
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No evidence text available
TleC affects MPND
| 2
MPND binds TleC. 2 / 2
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"Furthermore, the crystal structures of TleC and MpnD complexed with the substrates suggested that the distance between the C-3 position of DMSPP and the C-7 position of 1 (3.3 A in TleC and 3.1 A in MpnD) is closer than that between the C-1 position of the prenyl substrates and the N-1 position of 1 (6.0 A in TleC and MpnD) (XREF_SUPPLEMENTARY)."

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"The structural analysis of MpnD and TleC complexed with substrates suggested that the three amino acid residues Trp97, Phe170, and Ala173 in TleC and Tyr80, Trp157, and Met159 in MpnD regulate the selectivity of the length of prenyl donor and regiospecificity of prenylation position."
MPND is modified
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MPND is phosphorylated on S181. 1 / 1
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No evidence text available
MPND is phosphorylated on S178. 1 / 1
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No evidence text available
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"Thus, TleC and MpnD clearly prefer to accept GPP and DMAPP as the prenyl donors, respectively."

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"First, the indolactam specific TleC and MpnD both accepted DMAPP (C 5) and GPP (C 10) as substrates to produce pendolmycin (3), and lyngbyatoxin A (2) and 5-geranylindolactam V (4) (2:4 = 26:1 for TleC; 2:4 = 1:3 for MpnD), respectively (XREF_FIG)."
MPND affects TleC
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MPND binds TleC. 2 / 2
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"Furthermore, the crystal structures of TleC and MpnD complexed with the substrates suggested that the distance between the C-3 position of DMSPP and the C-7 position of 1 (3.3 A in TleC and 3.1 A in MpnD) is closer than that between the C-1 position of the prenyl substrates and the N-1 position of 1 (6.0 A in TleC and MpnD) (XREF_SUPPLEMENTARY)."

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"The structural analysis of MpnD and TleC complexed with substrates suggested that the three amino acid residues Trp97, Phe170, and Ala173 in TleC and Tyr80, Trp157, and Met159 in MpnD regulate the selectivity of the length of prenyl donor and regiospecificity of prenylation position."
MPND affects DEG15
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MPND activates DEG15. 2 / 2
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"First, the indolactam specific TleC and MpnD both accepted DMAPP (C 5) and GPP (C 10) as substrates to produce pendolmycin (3), and lyngbyatoxin A (2) and 5-geranylindolactam V (4) (2:4 = 26:1 for TleC; 2:4 = 1:3 for MpnD), respectively (XREF_FIG)."

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"Thus, TleC and MpnD clearly prefer to accept GPP and DMAPP as the prenyl donors, respectively."
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Trichostatin A decreases the amount of MPND. 1 / 1
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No evidence text available
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Thioacetamide decreases the amount of MPND. 1 / 1
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No evidence text available
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Tetrachloroethene decreases the amount of MPND. 1 / 1
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No evidence text available
Sulforaphane affects MPND
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Sulforaphane decreases the amount of MPND. 1 / 1
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No evidence text available
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No evidence text available
Methylmercury chloride decreases the amount of MPND. 1 / 1
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No evidence text available
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Methapyrilene demethylates MPND. 1 / 1
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No evidence text available
Jinfukang affects MPND
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Jinfukang increases the amount of MPND. 1 / 1
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No evidence text available
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Hsa-miR-335-5p decreases the amount of MPND. 1 / 1
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No evidence text available
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Hsa-let-7b-5p decreases the amount of MPND. 1 / 1
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No evidence text available
Hexabromocyclododecane decreases the amount of MPND. 1 / 1
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No evidence text available
Genistein affects MPND
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Genistein increases the amount of MPND. 1 / 1
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No evidence text available
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Cyclosporin A decreases the amount of MPND. 1 / 1
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No evidence text available
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Copper(II) sulfate decreases the amount of MPND. 1 / 1
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No evidence text available
Copper atom affects MPND
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Copper atom increases the amount of MPND. 1 / 1
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No evidence text available
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Cobalt dichloride decreases the amount of MPND. 1 / 1
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No evidence text available
Clofibrate affects MPND
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Clofibrate decreases the amount of MPND. 1 / 1
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No evidence text available
Bisphenol A affects MPND
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No evidence text available
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No evidence text available
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Benzo[a]pyrene decreases the amount of MPND. 1 / 1
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No evidence text available
Atrazine affects MPND
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Atrazine decreases the amount of MPND. 1 / 1
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No evidence text available
Aflatoxin B1 affects MPND
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Aflatoxin B1 decreases the amount of MPND. 1 / 1
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No evidence text available
Abrine affects MPND
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Abrine decreases the amount of MPND. 1 / 1
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No evidence text available
USP36 affects MPND
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No evidence text available
USP30 affects MPND
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No evidence text available
MPND affects pPAJV643
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MPND activates pPAJV643. 1 / 1
| 1

eidos
"The mpnD gene encodes a prenyltransferase that , unlike TleC or LtxC , transfers a dimethylallyl group to the C-7 position of ILV to complete the biosynthesis of pendolmycin.7 Like the verification of tleC above , mpnD was cloned into the position of ltxC to create pPAJV643 ( ltxAB-mpnD ) ."
MPND affects active proteins
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MPND activates active proteins. 1 / 1
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eidos
"These results show that the codon-optimized tleC , tleD , and mpnD all produce active proteins with the expected activity when heterologously expressed in Anabaena 7120.9 , 15 This demonstrates that the native levels of transcriptional and translational regulation exerted when these genes occupy the same positions as ltxC ( tleC , mpnD ) and ltxD ( tleD ) in the ltx gene cluster are sufficient to produce proteins that efficiently catalyzed the desired reactions ; each protein completely converted its substrate to the expected product ( s ) because 1 was not observed in extracts of ltxABC-tleD or ltxAB-mpnD ."
MPND affects USP36
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No evidence text available
MPND affects USP30
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No evidence text available
MPND affects E7
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MPND binds E7. 1 / 1
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No evidence text available
MPND affects C5
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MPND activates C5. 1 / 1
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"This would be the reason why MpnD produces not only the C-7 ' reverse ' prenylation product 2 but also the C-5 ' normal ' prenylation product 4."

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"Interestingly, both TleC and MpnD also accepted farnesyl pyrophosphate (FPP) (C 15), geranylgeranyl pyrophosphate (GGPP) (C 20) and geranylfarnesyl pyrophosphate (GFPP) (C 25) as prenyl donors, to produce 5, 6 and 7, respectively (XREF_FIG and XREF_SUPPLEMENTARY)."
GSK-J4 affects MPND
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GSK-J4 decreases the amount of MPND. 1 / 1
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No evidence text available
E7 affects MPND
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MPND binds E7. 1 / 1
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No evidence text available
2-hydroxypropanoic acid decreases the amount of MPND. 1 / 1
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No evidence text available
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No evidence text available