IndraLab

Statements

WDR48 affects USP1
25 | 1 357 268
WDR48 binds USP1.
25 | 314 256
USP1 binds WDR48. 10 / 561
25 | 302 223
reach
"USP1 and UAF1 complex possesses potent deubiquitinase activity."
29138248
reach
"64 , 65 These agents inhibit the activity of USP1‐WDR48 complex in a non‐competitive manner."
32951278
sparser
"Interestingly, we note that not only USP1‐UAF1 is activated by DNA loading of PCNA, but also USP1 alone, which shows an increase in catalytic efficiency of from 2.3 mM −1  s −1 to 4.8 mM −1  s −1 ."
33619839
sparser
"USP1-UAF1 also acts on the mono-ubiquitinated form of the DNA polymerase processivity factor PCNA in translesion DNA synthesis (TLS) xref ."
31253762
biogrid
No evidence text available
28514442
reach
"This agreed with the native gel electrophoresis study suggesting that mutation of Ser313 of USP1 to Ala prevented formation of the USP1 and UAF1 complex, while the USP1 (S313D) mutant retains normal ability to form a complex with UAF1."
23116119
reach
"The formation of USP1 and UAF1 complex greatly stimulates the DUB activity of USP1."
23797609
sparser
"Our data support a model in which USP1 and UAF1 form a complex in the cytoplasm that subsequently translocates to the nucleus through import mediated by USP1 NLSs."
22701671
reach
"UAF1 and the USP1 and UAF1 complex are also important in viral infection and pathogenesis, particularly in viral genomic integration and replication."
29091922
reach
"However, like UAF1, neither USP1 nor the UAF1 and USP1 complex exerts any influence on the D-loop reaction (XREF_SUPPLEMENTARY; data not shown)."
27239033
USP1 binds WDR48 and BCHE. 5 / 5
| 5
reach
"The E1, UAF1, and USP1 complex is necessary for efficient replication of HPV11 episomes in U2OS cells."
30890612
reach
"The E1, UAF1, and USP1 complex contributes to the generation of HPV11 RIs produced by unidirectional replication."
30890612
reach
"We conclude that the E1, UAF1, and USP1 complex supports the processing of late bidirectional theta RIs and propose that after the separation of bidirectional theta daughter genomes, the formerly converged replication forks undergo BLM mediated restart, thus initiating the unidirectional theta replication of HPV genomes."
30890612
reach
"The importance of the E1, UAF1, and USP1 complex for efficient HPV replication has been previously reported; however, we demonstrated that the lower replication efficiency of mutant HPV11 genomes encoding a E1 protein defective for UAF1 binding is associated with decreased production of RIs generated by the unidirectional mechanism, while bidirectional theta replication was affected to a lesser degree (XREF_FIG)."
30890612
reach
"Additionally, a role for the E1, UAF1, and USP1 complex in the processing of late bidirectional theta RIs would explain why defective E1, UAF1, and USP1 complex formation has no effect on the progression of theta and unidirectional replication forks (XREF_FIG)."
30890612
TBK1 binds USP1 and WDR48. 5 / 5
| 5
sparser
"Mechanistically, USP1 and UAF1 bound to TBK1, removed its K48-linked polyubiquitination, and then reversed the degradation process of TBK1."
29138248
sparser
"Collectively, these results indicate that UAF1 and USP1 specifically bind to TBK1."
29138248
sparser
"Mechanistically, UAF1 and USP1 bound to TBK1, removed K48-linked polyubiquitination of TBK1, and then stabilized its expression."
29138248
sparser
"UAF1 and USP1 interacted with TBK1 following LPS stimulation ( xref )."
29138248
sparser
"UAF1 and USP1 interact with TBK1."
29138248
USP1 binds USP46, USP12, and WDR48. 5 / 5
| 5
sparser
"For example, WDR48 interacts with USP1, USP12, and USP46 to activate their DUB activity ( Cohn et al., 2009; Yin et al., 2015 )."
27880911
sparser
"HPV E1 helicase, which possesses DNA unwinding activity and enhances viral DNA replication, could form a complex with UAF1 and any one of USP1, USP12 and USP46."
35283827
sparser
"For example, WDR48 interacts with USP1, USP12 and USP46 to activate their DUB activity ( xref ; xref )."
27880911
sparser
"UAF1 constitutively binds to USP1, USP12, and USP46, and this binding greatly enhances their deubiquitinase activity."
33247121
sparser
"For example, USP1, USP12 and USP46 can interact with WD40-repeat protein USP1-associated factor 1 (UAF1), and UAF1 stimulates the catalytic activity of human USP1 through the formation of a tight complex [ xref ]."
29386062
USP1 binds RAD51AP1 and WDR48. 4 / 4
| 4
sparser
"Thus, even though DNA binding by either UAF1 or RAD51AP1 is sufficient for targeting the USP1-UAF1-RAD51AP1 DUB complex to DNA for FANCD2 deubiquitination, the DNA binding attribute of both UAF1 and RAD51AP1 is required for optimal DNA damage repair."
31253762
sparser
"UAF1-USP1 readily associates with RAD51AP1 to form a trimeric complex ( xref )."
27239033
sparser
"In line with our findings and with results from others , Wagner et al. showed that RAD51AP1 interacts with UAF1-USP1, but also with USP7 and USP11, DUBs with important roles in the cellular response[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28963981
sparser
"UAF1-USP1 readily associates with RAD51AP1 to form a trimeric complex ( Figure S3 F)."
27239033
USP1 binds WDR48 and 3A. 3 / 3
| 3
sparser
"Our biochemical reconstitution studies (Fig.  xref and Supplementary Fig.  xref ) revealed that, via its DNA binding activity, RAD51AP1 targets the DNA binding defective USP1-UAF1 3A or USP1-UAF1 11A complex to deubiquitinate FANCD2 on DNA."
31253762
sparser
"To ask if UAF1 DNA-binding is relevant for ID2 deubiquitination, we examined the mutant USP1-UAF1 3A and USP1-UAF1 11A complexes (Supplementary Fig.  xref ) in FANCD2 deubiquitination."
31253762
sparser
"Since RAD51AP1, which stably associates with UAF1 xref , also possesses DNA binding activity xref , it was of considerable interest to test whether it would restore to USP1-UAF1 3A and USP1-UAF1 11A mutant complexes the ability to deubiquitinate FANCD2 on DNA."
31253762
USP1 binds WDR48 and HCC. 3 / 3
| 3
sparser
"Moreover, we confirmed the interaction of USP1‐WDR48 in HCC cells using co‐immunoprecipitation."
32951278
sparser
"Validation of the interaction between USP1 and WDR48 in HCC cell lines."
32951278
sparser
"In addition, we confirmed the interaction between USP1 and WDR48 in HCC cell lines using co‐immunoprecipitation, and the results showed that USP1 interacted with WDR48 in MHCC97H and SK‐Hep‐1 cells (Figure  xref )."
32951278
USP1 binds WDR48 and Ser313. 2 / 2
| 2
reach
"To assess whether the stability of the USP1 and UAF1 complex was affected by Ser313 to Ala mutation in USP1, the wild-type and mutant USP and UAF1 complexes were analyzed using native gel electrophoresis."
23116119
reach
"To confirm that the interaction between USP1 and UAF1 is mediated by USP1 Ser313 phosphorylation, six-His-tagged USP1 was coexpressed with untagged UAF1 in insect cells."
23116119
USP1 binds WDR48 and WD40 domain. 2 / 2
| 2
reach
"USP1 forms a complex with UAF1 where its WD40 domain binds and stimulates the ubiquitin protease activity of USP1 to counteract the monoubiquitination of the FANCD2 and FANCI heterodimer [XREF_BIBR]."
30831436
reach
"The SLD2 region of UAF1 binds FANCI and the WD40 domain of UAF1 binds and stimulates USP1."
28923280
Ubiquitin binds USP1 and WDR48. 2 / 2
| 2
sparser
"Here we determine structures of human USP1-UAF1 with and without ubiquitin and bound to monoubiquitinated FANCI-FANCD2."
33795880
sparser
"A recent publication features the crystal structure of human USP1-UAF1 complex with and without ubiquitin, and one that is bound to monoubiquitinated FANCI-FANCD2 59 ."
35365626
USP1 binds USP46 and WDR48. 2 / 2
| 2
sparser
"Homologous amino acid motifs, mapping to the Fingers sub-domain, mediate binding of USP1 and USP46 to UAF1."
25744535
sparser
"Also, UAF1 can interact with USP1 and USP46 in addition to USP12 [ xref ]."
33498168
USP1 binds WDR48, PCNA, and ELG1. 1 / 1
| 1
sparser
"ELG1 (enhanced level of genomic instability 1) interacts with the USP1–UAF1 complex and supports USP1-mediated de-ubiquitination of PCNA [62] ."
28666590
Mutated USP1 binds WDR48. 1 / 1
| 1
reach
"Native gel electrophoresis of the wild-type and mutant USP1 and UAF1 complexes."
23116119
USP1 binds WDR48 and helicase. 1 / 1
| 1
sparser
"In this study, we showed that the previously described interaction between the viral E1 helicase and the cellular UAF1-USP1 complex is necessary for the completion of bidirectional replication and the subsequent initiation of the unidirectional replication mechanism."
30890612
USP1 binds PALB2, WDR48, and helicase. 1 / 1
| 1
sparser
"In this report, we provide evidence that the previously described interaction between the viral E1 helicase and the cellular UAF1-USP1 deubiquitinating enzyme complex, a member of the Fanconi anemia DNA damage response pathway, is required for the completion of the bidirectional theta replication of the HPV11 genome and the subsequent initiation of the unidirectional replication."
30890612
USP1 binds ATAD5 and WDR48. 1 / 1
| 1
sparser
"Furthermore, the ectopic expression of ATAD5 mutant protein (ATAD5 UAF1del) that does not interact with the USP1–UAF1 complex and results in defective PCNA deubiquitylation ( xref ; xref ) reduced chromatin-bound PCNA level similar to the ectopic expression of wild-type ATAD5 ( xref )."
23277426
USP1 binds WDR48 and FANCD2. 1 / 1
| 1
sparser
"Alternatively, FANCI ubiquitination may directly disrupt USP1‐UAF1 binding to FANCD2."
32510829
USP1 binds WDR48 and K48. 1 / 1
| 1
reach
"Collectively, these data indicate that the USP1 and UAF1 complex selectively removes K48 linked ubiquitination of TBK1."
29138248
USP1 binds WDR48 and ELG1. 1 / 1
| 1
sparser
"Clearly there will also be separate regulatory networks, for example ELG1 interacts with USP1-UAF1 and affects PCNA deubiquitylation, but not FANCD2 ubiquitylation status [ xref ]."
21605559
USP1 binds WDR48 and serine. 1 / 1
| 1
reach
"It has been shown that UAF1 activates USP1 through a potential active-site modulation, and the complex formation between USP1 and UAF1 is regulated by serine phosphorylation."
23797609
USP1 binds USP5, ATAD5, and WDR48. 1 / 1
| 1
sparser
"Intriguingly, human ATAD5 appears to respond differently from its yeast counterpart to PCNA modifications: whereas no preferential affinity for sumoylated PCNA was reported, Lee et al. observed an i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23688817
USP1 binds WDR48 and foci. 1 / 1
| 1
sparser
"The recruitment of USP1-UAF1 appears to be transient because ELG1 knockdown did not affect the levels of USP1 bound to chromatin, and in contrast to ELG1, neither USP1 nor UAF1 formed foci following DNA damage [ xref ]."
21640107
USP1 binds NLRP3 and WDR48. 1 / 1
| 1
sparser
"In vitro binding assays demonstrated that NLRP3 could directly interact with USP1 and UAF1 (Fig.  xref )."
33247121
WDR48 activates USP1.
| 1 40 12
WDR48 activates USP1. 10 / 58
| 1 39 12
reach
"In vitro, USP1 activity is greatly stimulated by USP1-associated factor 1 (UAF1; also known as WDR48), enhancing USP1 catalytic turnover (k ) but not affinity (K ) for monoubiquitylated substrates ."
28959952
reach
"WDR48 and UAF1 is an activator of USP1 and more recently has been shown to activate USP12 and USP46."
19615732
reach
"UAF1 increases the basicity of the USP1 catalytic histidine, increasing its potency as a general base."
25702870
sparser
"The activation of USP1 by UAF1 takes place through an allosteric mechanism where the catalytic turnover (k cat ) of the enzyme is increased several fold (Cohn et al , xref )."
33619839
reach
"For example, in contrast to the USP46 BL1 residue F258, which supports the tail of Ub, the corresponding USP12 residue F262 is pushed into the catalytic cleft and would collide with the substrate.Usin[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27373336
reach
"Its phosphorylation at Ser313 is essential for interacting with UAF1 and subsequent stimulation of USP1 activity [XREF_BIBR]."
28659380
sparser
"Mutation of Ser313 to Ala on USP1 abolished the complex formation between USP1 and UAF1, thus prevented the activation of USP1 by UAF1( xref )."
23797609
reach
"Previous work on UAF1-USP1 binding showed WD40 domain of UAF1 was sufficient to act as an adapter protein and activate the catalytic DUB activity of USP1, which on its own has very little intrinsic catalytic activity in-vitro ."
31685642
reach
"In vitro, USP1 activity is greatly stimulated by USP1-associated factor 1 (UAF1; also known as WDR48), enhancing USP1 catalytic turnover (k cat ) but not affinity (K m ) for monoubiquitylated substrates 74 ."
28959952
sparser
"Using ubiquitin-7-amido-4-methylcoumarin (Ub-AMC) as a substrate, we have previously shown that UAF1 activates USP1 mainly by increasing its catalytic turnover (k), instead of its affinity toward the [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27373336
WDR48 bound to USP1 activates USP1. 1 / 1
| 1
reach
"The formation of USP1 and UAF1 complex greatly stimulates the DUB activity of USP1."
23797609
WDR48 inhibits USP1.
| 2
WDR48 inhibits USP1. 2 / 2
| 2
reach
"In vitro, USP1 activity is greatly stimulated by USP1-associated factor 1 (UAF1; also known as WDR48), enhancing USP1 catalytic turnover (k cat ) but not affinity (K m ) for monoubiquitylated substrates 74 ."
28959952
reach
"In vitro, USP1 activity is greatly stimulated by USP1-associated factor 1 (UAF1; also known as WDR48), enhancing USP1 catalytic turnover (k ) but not affinity (K ) for monoubiquitylated substrates ."
28959952
WDR48 deubiquitinates USP1.
| 1
WDR48 deubiquitinates USP1. 1 / 1
| 1
reach
"The E1 protein from anogenital HPV types interacts with the UAF1 associated deubiquitinating enzymes USP1, USP12, and USP46 to stimulate replication of the viral genome."
25833051
USP1 affects WDR48
25 | 1 323 256
USP1 binds WDR48.
25 | 314 256
USP1 binds WDR48. 10 / 561
25 | 302 223
reach
"USP1 and UAF1 complex possesses potent deubiquitinase activity."
29138248
reach
"64 , 65 These agents inhibit the activity of USP1‐WDR48 complex in a non‐competitive manner."
32951278
sparser
"Interestingly, we note that not only USP1‐UAF1 is activated by DNA loading of PCNA, but also USP1 alone, which shows an increase in catalytic efficiency of from 2.3 mM −1  s −1 to 4.8 mM −1  s −1 ."
33619839
sparser
"USP1-UAF1 also acts on the mono-ubiquitinated form of the DNA polymerase processivity factor PCNA in translesion DNA synthesis (TLS) xref ."
31253762
biogrid
No evidence text available
28514442
reach
"This agreed with the native gel electrophoresis study suggesting that mutation of Ser313 of USP1 to Ala prevented formation of the USP1 and UAF1 complex, while the USP1 (S313D) mutant retains normal ability to form a complex with UAF1."
23116119
reach
"The formation of USP1 and UAF1 complex greatly stimulates the DUB activity of USP1."
23797609
sparser
"Our data support a model in which USP1 and UAF1 form a complex in the cytoplasm that subsequently translocates to the nucleus through import mediated by USP1 NLSs."
22701671
reach
"UAF1 and the USP1 and UAF1 complex are also important in viral infection and pathogenesis, particularly in viral genomic integration and replication."
29091922
reach
"However, like UAF1, neither USP1 nor the UAF1 and USP1 complex exerts any influence on the D-loop reaction (XREF_SUPPLEMENTARY; data not shown)."
27239033
USP1 binds WDR48 and BCHE. 5 / 5
| 5
reach
"The E1, UAF1, and USP1 complex is necessary for efficient replication of HPV11 episomes in U2OS cells."
30890612
reach
"The E1, UAF1, and USP1 complex contributes to the generation of HPV11 RIs produced by unidirectional replication."
30890612
reach
"We conclude that the E1, UAF1, and USP1 complex supports the processing of late bidirectional theta RIs and propose that after the separation of bidirectional theta daughter genomes, the formerly converged replication forks undergo BLM mediated restart, thus initiating the unidirectional theta replication of HPV genomes."
30890612
reach
"The importance of the E1, UAF1, and USP1 complex for efficient HPV replication has been previously reported; however, we demonstrated that the lower replication efficiency of mutant HPV11 genomes encoding a E1 protein defective for UAF1 binding is associated with decreased production of RIs generated by the unidirectional mechanism, while bidirectional theta replication was affected to a lesser degree (XREF_FIG)."
30890612
reach
"Additionally, a role for the E1, UAF1, and USP1 complex in the processing of late bidirectional theta RIs would explain why defective E1, UAF1, and USP1 complex formation has no effect on the progression of theta and unidirectional replication forks (XREF_FIG)."
30890612
TBK1 binds USP1 and WDR48. 5 / 5
| 5
sparser
"Mechanistically, USP1 and UAF1 bound to TBK1, removed its K48-linked polyubiquitination, and then reversed the degradation process of TBK1."
29138248
sparser
"Collectively, these results indicate that UAF1 and USP1 specifically bind to TBK1."
29138248
sparser
"Mechanistically, UAF1 and USP1 bound to TBK1, removed K48-linked polyubiquitination of TBK1, and then stabilized its expression."
29138248
sparser
"UAF1 and USP1 interacted with TBK1 following LPS stimulation ( xref )."
29138248
sparser
"UAF1 and USP1 interact with TBK1."
29138248
USP1 binds USP46, USP12, and WDR48. 5 / 5
| 5
sparser
"For example, WDR48 interacts with USP1, USP12, and USP46 to activate their DUB activity ( Cohn et al., 2009; Yin et al., 2015 )."
27880911
sparser
"HPV E1 helicase, which possesses DNA unwinding activity and enhances viral DNA replication, could form a complex with UAF1 and any one of USP1, USP12 and USP46."
35283827
sparser
"For example, WDR48 interacts with USP1, USP12 and USP46 to activate their DUB activity ( xref ; xref )."
27880911
sparser
"UAF1 constitutively binds to USP1, USP12, and USP46, and this binding greatly enhances their deubiquitinase activity."
33247121
sparser
"For example, USP1, USP12 and USP46 can interact with WD40-repeat protein USP1-associated factor 1 (UAF1), and UAF1 stimulates the catalytic activity of human USP1 through the formation of a tight complex [ xref ]."
29386062
USP1 binds RAD51AP1 and WDR48. 4 / 4
| 4
sparser
"Thus, even though DNA binding by either UAF1 or RAD51AP1 is sufficient for targeting the USP1-UAF1-RAD51AP1 DUB complex to DNA for FANCD2 deubiquitination, the DNA binding attribute of both UAF1 and RAD51AP1 is required for optimal DNA damage repair."
31253762
sparser
"UAF1-USP1 readily associates with RAD51AP1 to form a trimeric complex ( xref )."
27239033
sparser
"In line with our findings and with results from others , Wagner et al. showed that RAD51AP1 interacts with UAF1-USP1, but also with USP7 and USP11, DUBs with important roles in the cellular response[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28963981
sparser
"UAF1-USP1 readily associates with RAD51AP1 to form a trimeric complex ( Figure S3 F)."
27239033
USP1 binds WDR48 and 3A. 3 / 3
| 3
sparser
"Our biochemical reconstitution studies (Fig.  xref and Supplementary Fig.  xref ) revealed that, via its DNA binding activity, RAD51AP1 targets the DNA binding defective USP1-UAF1 3A or USP1-UAF1 11A complex to deubiquitinate FANCD2 on DNA."
31253762
sparser
"To ask if UAF1 DNA-binding is relevant for ID2 deubiquitination, we examined the mutant USP1-UAF1 3A and USP1-UAF1 11A complexes (Supplementary Fig.  xref ) in FANCD2 deubiquitination."
31253762
sparser
"Since RAD51AP1, which stably associates with UAF1 xref , also possesses DNA binding activity xref , it was of considerable interest to test whether it would restore to USP1-UAF1 3A and USP1-UAF1 11A mutant complexes the ability to deubiquitinate FANCD2 on DNA."
31253762
USP1 binds WDR48 and HCC. 3 / 3
| 3
sparser
"Moreover, we confirmed the interaction of USP1‐WDR48 in HCC cells using co‐immunoprecipitation."
32951278
sparser
"Validation of the interaction between USP1 and WDR48 in HCC cell lines."
32951278
sparser
"In addition, we confirmed the interaction between USP1 and WDR48 in HCC cell lines using co‐immunoprecipitation, and the results showed that USP1 interacted with WDR48 in MHCC97H and SK‐Hep‐1 cells (Figure  xref )."
32951278
USP1 binds WDR48 and Ser313. 2 / 2
| 2
reach
"To assess whether the stability of the USP1 and UAF1 complex was affected by Ser313 to Ala mutation in USP1, the wild-type and mutant USP and UAF1 complexes were analyzed using native gel electrophoresis."
23116119
reach
"To confirm that the interaction between USP1 and UAF1 is mediated by USP1 Ser313 phosphorylation, six-His-tagged USP1 was coexpressed with untagged UAF1 in insect cells."
23116119
USP1 binds WDR48 and WD40 domain. 2 / 2
| 2
reach
"USP1 forms a complex with UAF1 where its WD40 domain binds and stimulates the ubiquitin protease activity of USP1 to counteract the monoubiquitination of the FANCD2 and FANCI heterodimer [XREF_BIBR]."
30831436
reach
"The SLD2 region of UAF1 binds FANCI and the WD40 domain of UAF1 binds and stimulates USP1."
28923280
Ubiquitin binds USP1 and WDR48. 2 / 2
| 2
sparser
"Here we determine structures of human USP1-UAF1 with and without ubiquitin and bound to monoubiquitinated FANCI-FANCD2."
33795880
sparser
"A recent publication features the crystal structure of human USP1-UAF1 complex with and without ubiquitin, and one that is bound to monoubiquitinated FANCI-FANCD2 59 ."
35365626
USP1 binds USP46 and WDR48. 2 / 2
| 2
sparser
"Homologous amino acid motifs, mapping to the Fingers sub-domain, mediate binding of USP1 and USP46 to UAF1."
25744535
sparser
"Also, UAF1 can interact with USP1 and USP46 in addition to USP12 [ xref ]."
33498168
USP1 binds WDR48, PCNA, and ELG1. 1 / 1
| 1
sparser
"ELG1 (enhanced level of genomic instability 1) interacts with the USP1–UAF1 complex and supports USP1-mediated de-ubiquitination of PCNA [62] ."
28666590
Mutated USP1 binds WDR48. 1 / 1
| 1
reach
"Native gel electrophoresis of the wild-type and mutant USP1 and UAF1 complexes."
23116119
USP1 binds WDR48 and helicase. 1 / 1
| 1
sparser
"In this study, we showed that the previously described interaction between the viral E1 helicase and the cellular UAF1-USP1 complex is necessary for the completion of bidirectional replication and the subsequent initiation of the unidirectional replication mechanism."
30890612
USP1 binds PALB2, WDR48, and helicase. 1 / 1
| 1
sparser
"In this report, we provide evidence that the previously described interaction between the viral E1 helicase and the cellular UAF1-USP1 deubiquitinating enzyme complex, a member of the Fanconi anemia DNA damage response pathway, is required for the completion of the bidirectional theta replication of the HPV11 genome and the subsequent initiation of the unidirectional replication."
30890612
USP1 binds ATAD5 and WDR48. 1 / 1
| 1
sparser
"Furthermore, the ectopic expression of ATAD5 mutant protein (ATAD5 UAF1del) that does not interact with the USP1–UAF1 complex and results in defective PCNA deubiquitylation ( xref ; xref ) reduced chromatin-bound PCNA level similar to the ectopic expression of wild-type ATAD5 ( xref )."
23277426
USP1 binds WDR48 and FANCD2. 1 / 1
| 1
sparser
"Alternatively, FANCI ubiquitination may directly disrupt USP1‐UAF1 binding to FANCD2."
32510829
USP1 binds WDR48 and K48. 1 / 1
| 1
reach
"Collectively, these data indicate that the USP1 and UAF1 complex selectively removes K48 linked ubiquitination of TBK1."
29138248
USP1 binds WDR48 and ELG1. 1 / 1
| 1
sparser
"Clearly there will also be separate regulatory networks, for example ELG1 interacts with USP1-UAF1 and affects PCNA deubiquitylation, but not FANCD2 ubiquitylation status [ xref ]."
21605559
USP1 binds WDR48 and serine. 1 / 1
| 1
reach
"It has been shown that UAF1 activates USP1 through a potential active-site modulation, and the complex formation between USP1 and UAF1 is regulated by serine phosphorylation."
23797609
USP1 binds USP5, ATAD5, and WDR48. 1 / 1
| 1
sparser
"Intriguingly, human ATAD5 appears to respond differently from its yeast counterpart to PCNA modifications: whereas no preferential affinity for sumoylated PCNA was reported, Lee et al. observed an i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23688817
USP1 binds WDR48 and foci. 1 / 1
| 1
sparser
"The recruitment of USP1-UAF1 appears to be transient because ELG1 knockdown did not affect the levels of USP1 bound to chromatin, and in contrast to ELG1, neither USP1 nor UAF1 formed foci following DNA damage [ xref ]."
21640107
USP1 binds NLRP3 and WDR48. 1 / 1
| 1
sparser
"In vitro binding assays demonstrated that NLRP3 could directly interact with USP1 and UAF1 (Fig.  xref )."
33247121
USP1 activates WDR48.
| 1 7
USP1 activates WDR48. 8 / 9
| 1 7
reach
"Moreover, we verified that (i) UAF1-USP1 is no more effective than UAF1 in the D-loop reaction (XREF_SUPPLEMENTARY), and (ii) USP1 fails to restore functional synergy of the UAF1 EEA mutant with RAD51AP1 (XREF_SUPPLEMENTARY)."
27239033
reach
"Moreover, we verified that UAF1-USP1 is no more effective than UAF1 in the D-loop reaction and that USP1 fails to restore functional synergy of the UAF1 EEA mutant with RAD51AP1."
27239033
reach
"The possibility that the NLS inactivating mutations might simultaneously interfere with UAF1 binding can not be formally ruled out, but these results suggest that the inability of USP1 (235-408) to promote UAF1 relocation can not be ascribed to competition with the nuclear import machinery."
25744535
reach
"Further studies of the USP1/UAF1 inhibitors suggested that pimozide and GW7647 inhibit USP1/UAF1 by a non‐competitive mechanism and bind at a site other than the active site of USP1/UAF1 [113]."
24239534
eidos
"Moreover , WDR48 was significantly reduced by USP1 knockdown in MHCC97H and SK-Hep-1 cells ( Figure 10B , C , E , F ) ."
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"USP1, the major DUB of FANCD2 and FANCI, inactivates these two proteins mediated by the USP1 activating factor UAF1 once DNA damage repair is finished [XREF_BIBR]."
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"This assay is based on the nuclear accumulation of UAF1 induced by USP1 co-expression and, by using fluorescently tagged versions of these proteins, the results can be observed in live cells, before processing for microscopy analysis."
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"Moreover, WDR48 was significantly reduced by USP1 knockdown in MHCC97H and SK‐Hep‐1 cells (Figure 10B,C,E,F)."
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USP1 inhibits WDR48.
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USP1-E444K inhibits WDR48. 1 / 1
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"Likewise, mutation of USP1 E444K, analogous to the impactful USP46 E186K mutation, abrogated WDR48 binding and stimulation of USP1 deubiquitinase activity."
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USP1 inhibits WDR48. 1 / 1
| 1
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"An analogous mutation in USP1 similarly blocked WDR48 dependent activation."
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