IndraLab

Statements

TNFAIP3 binds TRAF6 and CYLD. 7 / 7
| 7
sparser
"Nonetheless, depletion of TRIP6 can significantly enhance the association of A20 or CYLD with TRAF6 in ovarian cancer cells and promote the binding of A20 to TRAF6 in glioblastoma cells, suggesting that targeting TRIP6 may prove to be an effective strategy to restore the function of A20 and CYLD in restricting the NF-κB activity in these cancer cells."
27134758
sparser
"To address this issue, we expressed FLAG-TRAF6 in HEK293T cells and treated cells with LPA for various times to determine how TRAF6 associates with deubiquitinase A20 or CYLD."
27134758
sparser
"Nonetheless, depletion of TRIP6 by either shRNA ( xref ) or Cas9/sgRNA ( xref ) not only enhanced the association of TRAF6 with A20, but also with CYLD in untreated and treated cells, suggesting a significant role for TRIP6 in antagonizing the binding of TRAF6 to both A20 and CYLD in ovarian cancer cells."
27134758
sparser
"On the other hand, in ovarian cancer cells and glioblastoma cells that show persistent NF-κB activity and high levels of TRIP6, both A20 and CYLD bind to TRAF6 very weakly."
27134758
sparser
"In this regard, here we provide evidence that the adaptor protein TRIP6 (thyroid hormone receptor-interacting protein 6), a specific interacting protein of the LPA2 receptor but not other LPA receptors, recruits TRAF6 to the LPA2 receptor and enhances the E3 ligase activity of TRAF6 by antagonizing the association of A20 and CYLD to TRAF6."
27134758
sparser
"Overexpression of TRIP6 interferes with the recruitment of A20 to TRAF6, whereas depletion of TRIP6 enhances the association of TRAF6 with A20 and CYLD, and eliminates LPA-promoted K63-linked polyubiquitination of TRAF6."
27134758
sparser
"In contrast, depletion of TRIP6 by TRIP6-specific shRNA or Cas9/sgRNA greatly enhances the association of TRAF6 with A20 and CYLD, and attenuates lysophosphatidic acid-induced muclear factor-κB and JNK/p38 activation in ovarian cancer cells."
27134758
PPP2 binds TNFAIP3, CYLD, PP2C, and protein phosphatase. 3 / 3
| 3
sparser
"Furthermore, IKKc has been implicated in interactions with protein phosphatases PP2A and PP2C as well as deubiquitinases A20 and CYLD (Solt & May, 2008; Liu et al., 2012) ."
25082354
sparser
"Furthermore, IKKγ has been implicated in interactions with protein phosphatases PP2A and PP2C as well as deubiquitinases A20 and CYLD (Solt & May, xref ; Liu et al ., xref )."
25082354
sparser
"Furthermore, IKKγ has been implicated in interactions with protein phosphatases PP2A and PP2C as well as deubiquitinases A20 and CYLD (Solt & May, 2008; Liu et al., 2012)."
25082354
TNFAIP3 binds ITCH and CYLD. 3 / 3
| 3
sparser
"A20 and Cyld can form independent complexes with Itch to disassemble K63 chains and to add K48 polyubiquitination ( xref , xref )."
31515257
sparser
"Both A20 and Cyld can independently associate with Itch and Cyld interacts with Cbl-b to facilitate the removal of K63 polyubiquitin chains and to add K48 polyubiquitination ( xref , xref , xref )."
31515257
sparser
"DUB-E3 interactions are used for mutual ubiquitin-dependent regulation (e.g., to control each other’s stability, see above) or for editing ubiquitin chain architecture on particular substrates (as shown for the hybrid DUB/E3 enzyme A20 and CYLD-ITCH complexes during inflammatory signaling [ xref , xref ])."
33335288
TNFAIP3 binds CYLD. 3 / 3
| 3
sparser
"Adding just another level of complexity to an already crowded CD137 signalosome, we have recently observed the functional association of K63-DUBs A20 and CYLD to the CD137 signalosome."
30524423
sparser
"IP assay results indicated that the methylated TRAF2 weakened the interaction with A20 or CYLD."
| PMC
sparser
"Furthermore, A20 and CYLD are recognized for their tumor suppressing functions because deficiency or mutation of A20 or CYLD is associated with lymphoma and familiar cylindromatosis, respectively [ xref – xref ]."
30471562
TNFAIP3 binds UBE2L3, OTULIN, and CYLD. 2 / 2
| 2
sparser
"DUBs can directly regulate E2 enzymes, xref which raises the question as to whether OTULIN, CYLD, or A20 interact with UBE2L3."
25640675
sparser
"DUBs can directly regulate E2 enzymes, 38 which raises the question as to whether OTULIN, CYLD, or A20 interact with UBE2L3."
25640675
TNFAIP3 binds NMRAL1 and CYLD. 2 / 2
| 1 1
sparser
"Several DUBs, including A20, CYLD and USP7, have been reported to downregulate NF- κ B. Co-IP assays were thus carried out to examine the interactions between these enzymes and HSCARG, and the results showed that HSCARG interacts weakly with A20 or CYLD but strongly interacts with USP7 ( xref , xref )."
24832601
reach
"Co-IP assays were thus carried out to examine the interactions between these enzymes and HSCARG, and the results showed that HSCARG interacts weakly with A20 or CYLD but strongly interacts with USP7 (XREF_FIG, XREF_SUPPLEMENTARY)."
24832601
TNFAIP3 binds USP25, DDX58, CYLD, and carD. 1 / 1
| 1
sparser
"The comparison between the deubiquitinase activities and binding affinities to RIG-I-CARD of USP21, A20, and CYLD indicate that USP21 is a bona fide RIG-I deubiquitinase, since among these three DUBs, USP21 is the only one that has the potential to inhibit RIG-I-CARD polyubiquitination both in vivo and in vitro and co-immunoprecipitates with RIG-I-CARD [ xref ]."
33138315
TNFAIP3 binds TNFRSF9, TRAF2, and CYLD. 1 / 1
| 1
sparser
"The deubiquitinases A20 and CYLD interact with the 4-1BB and TRAF2 complex and, by regulating the ubiquitination of TRAF2 and TAK1 ( xref ), they decrease 4-1BB activation [ xref ]."
| PMC
TNFAIP3 binds OTULIN and CYLD. 1 / 1
| 1
reach
"Indeed it is intriguing that OTULIN, A20 and CYLD either bind or hydrolyse Met1 linkages with some degree of specificity."
26503766
IKK_complex binds TNFAIP3 and CYLD. 1 / 1
| 1
sparser
"In this way, p47 negatively regulates IKKγ/NEMO independently of the other two known regulators of the IKK complex, A20 and CYLD ( xref , xref )."
33869030
TNFAIP3 binds RNF31, OTULIN, CYLD, and MYSM1. 1 / 1
| 1
sparser
"Upon immunoprecipitation, we observed no direct interaction between MYSM1 with HOIP, NEMO or the negative regulators CYLD, OTULIN or A20 (Fig.  xref )."
30405132