IndraLab

Statements

TRAF6 binds YOD1. 10 / 56

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"Further, we also show binding data in primary cells: YOD1 and TRAF6 interact in primary human umbilical vein endothelial cells (HUVEC) ( xref )."

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"To increase the physiological relevance, we show endogenous interaction of TRAF6 and YOD1 in different cell lines and also primary human umbilical vein endothelial cells (HUVEC) ( xref )."

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"We show that YOD1 binds to TRAF6 in unstimulated cells and that binding is decreased after IL-1 stimulation."

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"Indeed, co-IP experiments using MYC-TRAF6 together with Crimson-p62 and GFP-YOD1 revealed that YOD1 was able to inhibit the association of TRAF6 and p62, while p62 did not alter the binding of YOD1 to TRAF6 ( xref )."

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"Co-IP revealed binding of reconstituted YOD1 to TRAF6 in unstimulated cells and the interaction was reduced after IL-1β stimulation ( xref and xref )."

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"Thus, it is not strictly clear whether there is still residual endogenous YOD1 bound or an influence of the endogenous in the case of YOD1 C160S. We show decreased binding of YOD1 to TRAF6 after IL-1 stimulation in two reconstituted HeLa KO cell clones ( xref and xref ) and of endogenous TRAF6/YOD1 in HeLa cells and HUVEC ( xref ), providing evidence that interaction is reduced after IL-1 stimulation."

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"In contrast, YOD1 deubiquitinase directly associates with TRAF6 and competes with SQSTM1 for TRAF6 binding and activation in the NF-κB pathway in macrophages or cancer cells [50] ."

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TRAF6 binds YOD1 and SQSTM1. 3 / 3

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"By directly comparing the binding of YOD1 and p62 to TRAF6 in HEK293 cells we could confirm that both proteins are associating with the C-terminal MATH domain ( xref )."

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"Also, we quantified the amount of p62 and YOD1 bound to TRAF6 after double transfection (control, set to 1) and triple transfection (TRAF6, p62 and YOD1) in three independent experiments ( xref )."

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"Therefore, we investigated whether YOD1 and p62 are binding simultaneously to TRAF6 and potentially forming a tripartite complex or if binding of the two MATH domain interactors is mutually exclusive and possibly even competitive."

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YOD1 binds TRAF6, TRAF, and BLNK. 2 / 2

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"YOD1 binds to the C-terminal TRAF homology domain of TRAF6 that also serves as the interaction surface for the adaptor p62/Sequestosome-1, which is required for IL-1 signaling to NF-κB. We show that YOD1 competes with p62 for TRAF6 association and abolishes the sequestration of TRAF6 to cytosolic p62 aggregates by a non-catalytic mechanism."

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"In addition, YOD1 can bind to the C-terminal TRAF homology domain of TRAF6, which also serves as the interaction surface for the adaptor p62/Sequestosome-1required for IL-1 signaling to NF-κB. Interestingly, the NF-κB pathway is closely related to CAD xref , xref ."

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IL1 binds TRAF6 and YOD1. 2 / 2

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"Indeed, it seemed likely that in this overexpression scenario there was slightly augmented association of TRAF6 and YOD1 WT after short IL-1 stimulation."

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"In fact, IL-1-induced TRAF6 ubiquitination correlates with a decreased binding of YOD1 to TRAF6 after IL-1 stimulation (see xref and xref ), and we asked, whether reduced binding is also visible at the level of endogenous proteins in HeLa cells and primary HUVEC cells ( xref )."

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YOD1 inhibits TRAF6.

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YOD1 inhibits TRAF6. 4 / 6

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"Hence, our data define that YOD1 antagonizes TRAF6 and p62 dependent IL-1 signaling to NF-kappaB."

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"YOD1 antagonizes ubiquitin ligase TRAF6 and p62 dependent interleukin-1 signaling to NF-kappaB."

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"We show that YOD1 competes with p62 for TRAF6 association and abolishes the sequestration of TRAF6 to cytosolic p62 aggregates by a non catalytic mechanism."

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YOD1 ubiquitinates TRAF6.

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YOD1 leads to the ubiquitination of TRAF6. 1 / 1

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"Clearly, YOD1 deficiency alone is not sufficient to induce TRAF6 ubiquitination or IKK and NF-kappaB signaling in the absence of any stimulation."

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YOD1 affects cell population proliferation

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YOD1 activates cell population proliferation.

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YOD1 activates cell population proliferation. 10 / 18

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"In the present study, we have shown that knockdown of YOD1 by siRNA inhibited proliferation and migration of HOK cells in vitro."

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"We provide evidence for the first time that YOD1 contributes to changing the abilities of cell proliferation and migration regulated by TGF-β3 signaling pathway, which may be involved in the pathogenesis of NSCL/P.We explored the effects of YOD1 siRNA on the proliferation and migration of HOK cells."

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"The CCK-8 assay showed that knockdown of YOD1 by siRNA inhibited the HOK cells proliferation."

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"However, a recent study showed that downregulation of YOD1 may promote cell proliferation in cervical cancer cells [28]."

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"EDU and Flow Cytometry showed that high expression of YOD1 could significantly promote the proliferation of PAAD cells."

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"YOD1 promoted the proliferation of hepatocytes and led to hepatomegaly depending on the YAP/TAZ activity [36]."

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"Using a transgenic mouse model, we confirmed that the Dox inducible expression of YOD1 in the liver enhanced the proliferation of hepatocytes, and led to hepatomegaly in a YAP and TAZ-activity-dependent manner."

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"YOD1 overexpression promotes TNBC cell proliferation, migration and invasion."

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"Consistent with previous studies, we showed that overexpression of YOD1 enhanced osteosarcoma progression, including elevated cell proliferation, cell adhesion and inhibited cell apoptosis."

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"Several studies showed that YOD1 induced cancer progression by upregulating cell proliferation and inhibiting cell apoptosis partly via Yes Associated Protein 1 (YAP) signaling, such as liver cancer [[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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YOD1 inhibits cell population proliferation.

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YOD1 inhibits cell population proliferation. 10 / 17

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"Our findings suggest that the combined action of YOD1 and USP21 inhibits A549 cell proliferation via the Hippo signaling pathway."

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"Moreover, YOD1 inhibited cell proliferation induced by NEDD4 in kidney cells [ 86 ]."

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"YOD1 inhibits HNSCC cell proliferation in vitro."

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"Therefore, our results suggest that the repression of TGF-β3-mediated transcriptional activity mediated by YOD1 RNAi may inhibit cell proliferation and migration to induce NSCL/P.In conclusion, we have shown that the downregulation of YOD1 using RNAi inhibits proliferation and migration of HOK cells, which involve inhibiting the TGF-β3 signaling pathway."

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"In the EdU assay, Dox-induced YOD1 significantly inhibited cell proliferation (Fig. 2D)."

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"Additionally, YOD1 knockdown notably increased the proliferation of patient-derived renal cancer organoids, as well as ccRCC proliferation and metastasis, in vitro and in vivo."

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eidos

"However , a recent study showed that downregulation of YOD1 may promote cell proliferation in cervical cancer cells [ 28 ] ."

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"YOD1 inhibits ccRCC cell proliferation and metastasis."

40274778

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"Notably, YOD1 knockdown significantly promoted the proliferation of tumor organoids derived from ccRCC patients (Fig. 2G)."

40274778

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"Our findings indicate that YOD1 inhibits cell proliferation in A549 cells."

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YOD1 affects Circulin-C

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YOD1 binds Circulin-C. 10 / 28

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"Compared to controls, we found that the related expression of circ-YOD1 was significantly increased in CAD patients, but hsa-miR-21-3p and hsa-miR-296-3p were reduced (Fig. xref )."

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"Therefore, we hypothesized that circ-YOD1 could play a negative regulatory role on miR-21-3p/miR-296-3p expression in CAD."

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"After transfection with siRNA or the circ-YOD1 overexpression vector, circ-YOD1 expression was identified using qRT-PCR in THP-1 cells and HASMCs (Fig. xref )."

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"Accordingly, downregulation of circ-YOD1 expression could reduce the expression of BCL6 , FBXL18 , MMP9 and FCGR3B as shown by transfecting THP-1 cells and HASMCs with siRNAs (Fig. xref )."

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"The result of ROC curve analysis showed that the 95% confidence interval (CI) of circ-YOD1 was 0.745–0.904, and the AUC was 0.824."

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"The results of pairwise comparison showed a statistically significant difference for circ-YOD1 ( P YOD1-21 < 0.01; P YOD1-296 < 0.05, Fig. xref )."

31797949

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"Then, the expression levels of miR-21-3p and miR-296-3p were downregulated significantly in the overexpression circ-YOD1 group compared to the negative control."

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"Accordingly, downregulation of circ-YOD1 expression could increase the expression of miR-21-3p and miR-296-3p as analyzed after transfecting THP-1 cells and HASMCs with siRNAs against circ-YOD1 (Fig. xref )."

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"Thus, we performed an integrated bioinformatic analysis and speculated that circ-YOD1 may serve as a diagnostic or prognostic biomarker of CAD and have potential therapeutic value."

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"Thus, Circ-YOD1 may regulate parental gene YOD1 expression that leads to CAD."

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YOD1 affects CDK1

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YOD1 binds CDK1.

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YOD1 binds CDK1. 10 / 21

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"The result showed that FLAG was detectable when HA precipitated, and HA was also detectable when FLAG precipitated, demonstrating a direct interaction between YOD1 and CDK1(Fig. xref G)."

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"Meantime, recombinant GST-YOD1 and HIS-CDK1 proteins were purified, and the GST pulldown results further excluded the direct interaction between YOD1 and CDK1 ( Fig. xref )."

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"Next, we expressed full-length FLAG-YOD1 and different truncation mutants in HEK293 cells and co-immunoprecipitations using the anti-FLAG antibodies or anti-CDK1 antibodies confirmed that CDK1 bound to the Znf domain of YOD1, but not to the N-terminal UBX region and central otubain region of YOD1 (Fig. xref C)."

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"Congruent with the above results, CDK1 specifically bound to the C-terminal Znf domain of YOD1 rather than the N-terminal UBX region and central otubain region of YOD1 (Fig. xref D)."

37667382

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"Specifically, YOD1 binds directly to CDK1, leading to de-polyubiquitylation and upregulation of CDK1 expression [ xref ]."

40133267

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"These findings indicate that CDK1 bound to the Znf domain of YOD1 and then YOD1-T-OTUD was the Ub-specific domain of YOD1 to deubiquitinate CDK1 while the N-terminal domain had no deubiquitinating effect."

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"In addition, CDK1 inhibitors could eliminate the effect of YOD1 overexpression (the proliferation and resistance to cisplatin), which further elucidates the tumor promoting mechanism of the YOD1-CDK1 axis."

37667382

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"The UBXL domain of YOD1 is essential for binding TRAF6 and p97, and YOD1 interacts with CDK1 via the C-terminal ZnF domain (Ernst et al, xref ; Han et al, xref ; Schimmack et al, xref )."

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"For example, the CDK1 inhibitor can eliminate the effects of YOD1 overexpression (proliferation and resistance to cisplatin), thereby elucidating the tumor-promoting mechanism of the YOD1-CDK1 axis [ xref ]."

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"Specifically, YOD1 binds directly to CDK1, leading to de-polyubiquitylation and upregulation of CDK1 expression [37]."

eidos

"Finally , in vivo study demonstrated that YOD1 knockdown or YOD1 inhibitor could inhibit CDK1 expression and suppress the growth and metastasis of TNBC tumors ."

37667382

YOD1 deubiquitinates CDK1.

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YOD1 deubiquitinates CDK1. 1 / 1

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"YOD1 deubiquitylates CDK1 and maintains CDK1 stability."

37667382

Circulin-C affects YOD1

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YOD1 binds Circulin-C. 10 / 28

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"Compared to controls, we found that the related expression of circ-YOD1 was significantly increased in CAD patients, but hsa-miR-21-3p and hsa-miR-296-3p were reduced (Fig. xref )."

31797949

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sparser

"Therefore, we hypothesized that circ-YOD1 could play a negative regulatory role on miR-21-3p/miR-296-3p expression in CAD."

31797949

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sparser

"After transfection with siRNA or the circ-YOD1 overexpression vector, circ-YOD1 expression was identified using qRT-PCR in THP-1 cells and HASMCs (Fig. xref )."

31797949

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sparser

"Accordingly, downregulation of circ-YOD1 expression could reduce the expression of BCL6 , FBXL18 , MMP9 and FCGR3B as shown by transfecting THP-1 cells and HASMCs with siRNAs (Fig. xref )."

31797949

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sparser

"The result of ROC curve analysis showed that the 95% confidence interval (CI) of circ-YOD1 was 0.745–0.904, and the AUC was 0.824."

31797949

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sparser

"The results of pairwise comparison showed a statistically significant difference for circ-YOD1 ( P YOD1-21 < 0.01; P YOD1-296 < 0.05, Fig. xref )."

31797949

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"Then, the expression levels of miR-21-3p and miR-296-3p were downregulated significantly in the overexpression circ-YOD1 group compared to the negative control."

31797949

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sparser

31797949

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sparser

"Thus, we performed an integrated bioinformatic analysis and speculated that circ-YOD1 may serve as a diagnostic or prognostic biomarker of CAD and have potential therapeutic value."

31797949

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sparser

"Thus, Circ-YOD1 may regulate parental gene YOD1 expression that leads to CAD."

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YOD1 affects NLRP3

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YOD1 binds NLRP3.

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YOD1 binds NLRP3. 10 / 18

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"YOD1 interacted with NLRP3."

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"To verify the MS results and determine whether YOD1 interacted with other NLRP3 inflammasome molecules, we overexpressed Myc-tagged YOD1 with Flag-tagged NLRP3 or Flag-tagged Caspase-1 or Flag-tagged ASC in HEK293T cells, respectively (Figs. xref and xref )."

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"Deubiquitinase YOD1 interacts with NLRP3, leading to the inhibition of NLRP3 inflammasome activation by removing a specific ubiquitin chain [ xref ]."

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"As expected, the interaction between YOD1 and NLRP3 remained robust after mutation (Fig. 7B, C)."

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"Our previous results demonstrated that YOD1 specially interacted with NLRP3 and inhibited NLRP3 inflammasome activation, however, the functional relationship between YOD1, NLRP3 inflammasome and blood clotting remained unknown."

38789414

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"As expected, the interaction between YOD1 and NLRP3 remained robust after mutation (Fig. xref )."

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"Using MS, co-IP and co-immunofluorescence data, we identified NLRP3 as a putative target protein and YOD1 specifically interacted with NLRP3, but not with ASC and Caspase-1."

38789414

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"In our study, we showed that YOD1 interacted with NLRP3 and suppressed the NLRP3 inflammasome activation."

38789414

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"YOD1 interacted with NLRP3 to remove K33-linked ubiquitination of NLRP3 based on its deubiquitinating enzyme activity and specifically inhibited expression of NLRP3 as well as activation of NLRP3 inflammasome."

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"Although the mutation of YOD1 could interact with NLRP3, we found that the effect of YOD1 on NLRP3 ubiquitination was dependent on the deubiquitinase catalytic activity."

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YOD1 decreases the amount of NLRP3.

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YOD1 decreases the amount of NLRP3. 7 / 7

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"YOD1 attenuated NLRP3 expression and inflammation activation in vitro and in vivo."

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"Intriguingly, overexpression of YOD1 greatly decreased NLRP3 protein level rather than NLRP3 mRNA expression in MRSA-infected PMs, suggesting that YOD1 regulated NLRP3 expression through post-translational modifications (Fig. S4A–C)."

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"However, the protein level of NLRP3 induced by MRSA decreased in a dosage-dependent after Yod1 was transfected into macrophages, whereas Yod1(C155S) did not affect NLRP3 expression (Fig. 7D, E), indicating that YOD1 suppressed NLRP3 expression through its enzymatic activity."

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"Furthermore, the loss of Yod1 expression enhanced the expression of NLRP3 in vitro and in vivo."

38789414

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"Conversely, overexpression of YOD1 decreased the expression of NLRP3 in MRSA-infected PMs."

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"Furthermore, ectopic expression of Yod1 in PMs inhibited the expression of NLRP3, while the mutant did not."

38789414

YOD1 deubiquitinates NLRP3.

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YOD1 leads to the deubiquitination of NLRP3. 2 / 2

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"6A and S4D, E, YOD1 significantly reduced the ubiquitination of NLRP3, while ASC and Caspase-1 did not, suggesting that YOD1 inhibited NLRP3 inflammasome activation by cleaving NLRP3 ubiquitination."

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"However, compared with the control group, overexpression of Yod1 abrogated WT ubiquitination of NLRP3 (Fig. 6B), while knockout of Yod1 significantly enhanced them (Fig. 6C)."

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CDK1 affects YOD1

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CDK1 binds YOD1.

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YOD1 binds CDK1. 10 / 21

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"The result showed that FLAG was detectable when HA precipitated, and HA was also detectable when FLAG precipitated, demonstrating a direct interaction between YOD1 and CDK1(Fig. xref G)."

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"Meantime, recombinant GST-YOD1 and HIS-CDK1 proteins were purified, and the GST pulldown results further excluded the direct interaction between YOD1 and CDK1 ( Fig. xref )."

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"Congruent with the above results, CDK1 specifically bound to the C-terminal Znf domain of YOD1 rather than the N-terminal UBX region and central otubain region of YOD1 (Fig. xref D)."

37667382

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"Specifically, YOD1 binds directly to CDK1, leading to de-polyubiquitylation and upregulation of CDK1 expression [ xref ]."

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"The UBXL domain of YOD1 is essential for binding TRAF6 and p97, and YOD1 interacts with CDK1 via the C-terminal ZnF domain (Ernst et al, xref ; Han et al, xref ; Schimmack et al, xref )."

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"Specifically, YOD1 binds directly to CDK1, leading to de-polyubiquitylation and upregulation of CDK1 expression [37]."

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YOD1 binds CDK1 and RPS6. 1 / 1

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"Meantime, recombinant GST-YOD1 and HIS-CDK1 proteins were purified, and the GST pulldown results further excluded the direct interaction between YOD1 and CDK1 ( Fig. S6)."

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CDK1 increases the amount of YOD1.

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CDK1 increases the amount of YOD1. 1 / 1

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"Furthermore, CDK1 inhibitors (RO3360 4 μm and CDK1-IN-4 2 μm) were used and the CCK8 results showed that CDK1 inhibition completely eliminated the proliferative effect of YOD1 overexpression."

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YOD1 affects ZNF24

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YOD1 binds ZNF24.

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YOD1 binds ZNF24. 10 / 13

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"We then conducted reciprocal coimmunoprecipitation (co-IP) experiments, which corroborated the mass spectrometry findings, revealing an interaction of endogenous YOD1 and ZNF24 in cells (Fig. xref )."

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"In addition, an in vitro Glutathione S-transferase (GST) pull-down assay revealed that the purified protein HIS-YOD1 directly bound to GST-ZNF24 but not to the GST control (Fig. xref ), thereby verifying a direct interaction between YOD1 and ZNF24."

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"Interaction of the SCAN box domain of ZNF24 with the UBX-like domain of YOD1."

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"To gain further insight into the regions necessary for the binding of YOD1 to ZNF24, we generated truncations of YOD1 (Fig. xref ) and ZNF24 (Fig. xref )."

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"Our study demonstrated for the first time that YOD1 interacted with and stabilized ZNF24, with the UBX-like domain of YOD1 binding to the SCAN box domain of ZNF24."

40274778

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"Coimmunoprecipitation assays demonstrated that both endogenous and exogenous YOD1 interact with the N-terminal SCAN box region of ZNF24 (Fig. xref )."

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"Mechanistically, YOD1 binds directly to and stabilizes ZNF24, specifically removing the K27/29/48-linked ubiquitination of ZNF24 at the K308 site."

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"Thus, we hypothesized that YOD1 may affect ccRCC progression through the ZNF24/VEGF axis.We then conducted reciprocal coimmunoprecipitation (co-IP) experiments, which corroborated the mass spectrometry findings, revealing an interaction of endogenous YOD1 and ZNF24 in cells (Fig. 3E, F)."

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"In addition, an in vitro Glutathione S-transferase (GST) pull-down assay revealed that the purified protein HIS-YOD1 directly bound to GST-ZNF24 but not to the GST control (Fig. 3H), thereby verifying a direct interaction between YOD1 and ZNF24."

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"To gain further insight into the regions necessary for the binding of YOD1 to ZNF24, we generated truncations of YOD1 (Fig. 4A) and ZNF24 (Fig. 4F)."

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YOD1 deubiquitinates ZNF24.

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YOD1 deubiquitinates ZNF24. 5 / 5

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"Our results reveal the mechanism by which YOD1 regulates VEGFA transcription and suppresses tumorigenesis by deubiquitinating ZNF24, providing a therapeutic target in ccRCC."

40274778

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"Given that YOD1 is a component of the OTU family of DUBs, we investigated whether YOD1 deubiquitinates ZNF24."

40274778

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"Moreover, ZNF24 polyubiquitination was dose-dependently suppressed by overexpression of wild-type YOD1 (Fig. 5B)."

40274778

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"Conversely, YOD1 knockdown significantly increased the level of ZNF24 polyubiquitination in both HEK-293T cells and ccRCC cell lines (A498 and 786-O) (Fig. 5D–F)."

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"Notably, the deubiquitination of ZNF24 by YOD1 was dependent on its OTU enzyme active site, as the YOD1-C160S mutant did not deubiquitinate ZNF24."

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YOD1 inhibits ZNF24.

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YOD1 inhibits ZNF24. 4 / 4

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"Additionally, the destabilization of the ZNF24 protein induced by YOD1 suppression was shown to be dependent on proteasome and was abolished following treatment with MG132, a proteasome inhibitor (Fig. 6E, F)."

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"Notably, in protein half-life experiments, overexpression of wild-type YOD1 significantly slowed the degradation of ZNF24 compared to the control group, whereas ZNF24 degradation in the YOD1-C160S overexpression group was similar to that of the control (Fig. 6G, H)."

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"Our findings indicated that YOD1 knockdown in ccRCC cells decreased the protein stability of ZNF24, leading to attenuated transcriptional repression of VEGFA, which resulted in increased VEGFA expression and promoted angiogenesis."

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"The deubiquitinase YOD1 suppresses tumor progression by stabilizing ZNF24 in clear cell renal carcinoma."

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YOD1 affects USP25

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YOD1 binds USP25.

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YOD1 binds USP25. 10 / 15

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"YOD1 binds to USP21."

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"YOD1 binds to USP21."

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"Subsequently, we investigated the interaction between YOD1 and USP21, and our findings demonstrate that YOD1 indeed binds to USP21 (Fig. 1B, C)."

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"After identifying the binding between YOD1 and USP21, we aimed to investigate which protein acts as a DUB on the other."

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"Subsequently, we investigated the interaction between YOD1 and USP21, and our findings demonstrate that YOD1 indeed binds to USP21 (Fig. xref B, C)."

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"To accomplish this, we utilized the PPI database and bioinformatics tools (BioGRID, STRING, and Cytoscape) and discovered a cellular interaction between YOD1 and USP21 through co-IP, GST pull-down, and ICC assays (Fig. xref A–D)."

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"Our study also sheds light on the interaction between YOD1 and USP21, demonstrating the possibility of deubiquitination of one DUB by another DUB."

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"In summary, our study revealed several notable interactions between YOD1 and USP21."

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"Our study also sheds light on the interaction between YOD1 and USP21, demonstrating the possibility of deubiquitination of one DUB by another DUB."

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YOD1 activates USP25.

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YOD1 activates USP25. 5 / 5

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"Conversely, the overexpression of YOD1 increased USP21 protein stability in HEK293T, HeLa, and A549 cell lines (Fig. 4A, B)."

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"Specifically, we observed that YOD1 and USP21 have the ability to deubiquitinate each other, and that YOD1 can enhance the stability of USP21, while the reverse does not hold true."

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"Furthermore, YOD1 and USP21 deubiquitinate each other, with YOD1 upregulating USP21 protein stability, but not the reverse."

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"YOD1 upregulates USP21 stability, but not vice versa."

37743467

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reach

"Our results demonstrated that YOD1 increased the stability of USP21 in all three cell lines (Fig. 4A, B)."

37743467

NLRP3 affects YOD1

| 2 18

NLRP3 binds YOD1.

| 1 17

YOD1 binds NLRP3. 10 / 18

| 1 17

➶

sparser

"YOD1 interacted with NLRP3."

38789414

➶

sparser

38789414

➶

sparser

"Deubiquitinase YOD1 interacts with NLRP3, leading to the inhibition of NLRP3 inflammasome activation by removing a specific ubiquitin chain [ xref ]."

39201704

➶

reach

"As expected, the interaction between YOD1 and NLRP3 remained robust after mutation (Fig. 7B, C)."

38789414

➶

sparser

38789414

➶

sparser

"As expected, the interaction between YOD1 and NLRP3 remained robust after mutation (Fig. xref )."

38789414

➶

sparser

"Using MS, co-IP and co-immunofluorescence data, we identified NLRP3 as a putative target protein and YOD1 specifically interacted with NLRP3, but not with ASC and Caspase-1."

38789414

➶

sparser

"In our study, we showed that YOD1 interacted with NLRP3 and suppressed the NLRP3 inflammasome activation."

38789414

➶

sparser

1 | 6 6

YOD1 binds SYVN1. 10 / 14

1 | 6 6

➶

sparser

"Our coimmunoprecipitation experiments revealed that YOD1 binds to Hrd1 via Hrd1’s cytosolic domain."

24068323

➶

reach

"YOD1 can bind to HRD1, and HRD1 as an ER associated E3 is crucial in the process of reverse transport."

33546413

➶

sparser

"Whether the YOD1-Hrd1 interaction reflects a direct interaction or an interaction mediated by Hrd1 adaptors is unknown."

24068323

➶

reach

"Consistent with this idea, a deubiquitinase called YOD1 binds to Hrd1 and negatively regulates CTA1 retro translocation."

26362261

➶

sparser

"WT Hrd1-Myc coprecipitated with FLAG-WT YOD1 only when FLAG-WT YOD1was expressed in cells ( xref , top and second panels, compare lane 2 with lane 1), indicating that Hrd1 binds to YOD1."

24068323

➶

sparser

"Both WT Hrd1-Myc and cyt Hrd1-Myc but not TM1-6 Hrd1-Myc coprecipitated with FLAG-WT YOD1 ( xref , top three panels, compare lanes 1 and 2 with lane 3), demonstrating that Hrd1’s cytosolic domain interacts with YOD1."

24068323

➶

sparser

"This finding indicates that gp78 does not engage YOD1, and demonstrates specificity of the YOD1-Hrd1 interaction."

24068323

➶

biogrid

No evidence text available

| 18

➶

sparser

"In order to exclude the impact of plasmid toxicity on cell growth, we established cell lines expressing doxycycline-induced Flag-YOD1 (Fig. xref )."

37573347

➶

sparser

"In a reciprocal immunoprecipitation experiment, overexpressed HA-tagged PML/RAR α could also be detected in the immunocomplex of YOD1-Flag ( xref B)."

35847510

➶

sparser

"To determine the substrate proteins regulated by YOD1 in microglia, we transfected NIH/3T3 cells with empty Flag vector or Flag-YOD1 vector and performed immunoprecipitation assay combined with liquid chromatography-tandem mass spectrometry (LC–MS/MS) method to analyze potential substrate proteins of YOD1 ( xref A)."

40041897

➶

sparser

"Considering that YOD1 is a deubiquitinating enzyme, we transfected a Flag-YOD1 overexpression plasmid into 786-O cells."

40274778

➶

sparser

"We further co-transfected U2OS cells with Flag-YOD1 and Myc-p53 and performed immunoprecipitation using an anti-Myc tag antibody."

37454155

➶

sparser

"As expected, we detected clear binding of Myc-p53 to Flag-YOD1 (Fig. xref )."

37454155

➶

sparser

"To clarify whether YOD1 can regulate the ubiquitination of PKM2, we co‐transfected HA‐Ub, His‐PKM2 and Flag‐YOD1 or Flag‐CN overexpression plasmids into NIH3T3 cell lines or PC12 cells, and used His magnetic beads to perform Co‐IP experiments, and western blot to detect the ubiquitination of PKM2."

40681475

➶

sparser

"As shown in Fig. xref , Flag-YOD1 and HA-NCOA4 were transfected into HPAEpiC cells as indicated; YOD1 and NCOA4 directly bind to each other under LPS stimulation."

41326673

➶

sparser

"To do this, we co-transfected HA-Ub, His-MYH9, and Flag-YOD1 plasmids in NIH/3T3 cells respectively."

40041897

➶

sparser

"We co-transfected His-MYH9, Flag-YOD1 and mutant ubiquitin plasmids in NIH/3T3 cells respectively, retaining only the K48 and K63 active sites."

40041897

P97 affects YOD1

| 15

P97 binds YOD1. 10 / 13

| 13

➶

sparser

"In aggregate, YOD1 interacts directly with p97 and is a novel constituent of the ER dislocation machinery, thereby placing it in a perfect position to process ubiquitinated dislocation substrates in association with p97."

19818707

➶

sparser

"YOD1, the mammalian homolog of Otu1, also interacts with p97 through its UBX domain [ xref ]."

28159894

➶

sparser

"Overexpression of SCF FBXO27 promotes K48-linked ubiquitylation of LAMP2 and to a lesser extent LAMP1 [ xref ], but whether this E3 ligase is responsible for assembly of K48-linked chains that are removed by p97-YOD1 is unknown ( xref )."

36713230

➶

sparser

"To confirm that p97 and YOD1 form a complex in a cellular context, we transfected FLAG-tagged YOD1 variants, followed by preparation of detergent extracts."

19818707

➶

sparser

"In addition, we confirmed binding of recombinant YOD1 to p97 by GST-PD ( xref )."

28244869

➶

sparser

"In addition, p97 binds to a deubiquitinating enzyme YOD1, presumably so that polyubiquitin chains will not interfere with insertion into the proteasome ( xref )."

25071743

➶

sparser

"YOD1 interacts with p97 via its UBX domain."

21468305

➶

sparser

"We showed that p97 associates with YOD1 by virtue of its UBX domain and that YOD1 participates in a p97 complex that also contains NPL4 and UFD1 ( xref and xref ), involved in the dislocation of misfolded proteins from the ER (Ye et al., xref , xref )."

19818707

➶

sparser

"Importantly, a deletion variant of YOD1 (ΔUBX-GFP YOD1) that does not bind p97 still co-precipitated UBXD8 ( xref )."

19818707

➶

sparser

"The UBX-like domain of OTUD2 binds AAA+ ATPase p97, an important Ub-dependent regulator of protein homeostasis ( xref )."

27066941

YOD1 binds NPLOC4, Ufd1, and p97. 1 / 1

| 1

➶

sparser

"In a screen for potential functional partners of YOD1, Ernst et al. discovered an interaction between YOD1 and the p97-Ufd1-Npl4 complex, linking this DUB to the ERQC pathway [ xref ]."

22812527

YOD1 binds Ubx and p97. 1 / 1

| 1

➶

sparser

"The DUB YOD1, which binds to p97 via its UBX (ubiquitin regulatory X) domain, is also associated with ERAD [ xref ]."

28819009

YOD1 affects VCP

11 | 4

YOD1 binds VCP.

11 | 3

YOD1 binds VCP. 10 / 14

11 | 3

➶

biogrid

No evidence text available

24417208

➶

biogrid

No evidence text available

24417208

➶

reach

"OTUD2 orthologues bind to the AAA+ ATPase/unfoldase p97/VCP and furnish it with ubiquitin linkage processing or editing capability (Rumpf & Jentsch, 2006; Messick et al, 2008; Ernst et al, 2009)."

36958824

➶

biogrid

No evidence text available

28244869

➶

biogrid

No evidence text available

27753622

➶

biogrid

No evidence text available

19818707

➶

biogrid

No evidence text available

37667382

➶

reach

"Yod1, which is specifically associated with macro-autophagy, and codes for the cofactor YOD1, which binds with p97 to promote lysosomal clearance (Papadopoulos et al., 2017), was significantly increased in the FMD 3xTg male cohort compared with male 3xTg controls, although this effect was not observed in female 3xTg mice (Table S1; Data S1, page 47)."

36170815

➶

biogrid

No evidence text available

27753622

➶

biogrid

No evidence text available

32296183

YOD1 activates VCP.

| 1

YOD1 activates VCP. 1 / 1

| 1

➶

reach

"16 , 17 , 18 OTUD2 and VCPIP modulate the p97‐related pathway."

35098684

YOD1 affects TP53

2 | 8 5

YOD1 binds TP53.

2 | 5 5

YOD1 binds TP53. 10 / 12

2 | 5 5

➶

sparser

"We then confirmed that YOD1 interacts with p53 and stabilizes p53 via deubiquitination."

37454155

➶

sparser

"We further used IP to investigate the potential interaction between YOD1 and p53."

37454155

➶

sparser

"Taken together, these data indicated a specific interaction between YOD1 and p53."

37454155

➶

sparser

"To identify domains in p53 that interact with YOD1, we constructed four p53 mutants, including C-segment deletion (△C), MDM2 binding site deletion (△M), DBD domain alone (DBD), and N-terminal deletion (△N) (Fig. xref )."

37454155

➶

reach

"We then confirmed that YOD1 interacts with p53 and stabilizes p53 via deubiquitination."

37454155

➶

reach

"These results indicate that YOD1 stabilizes the p53 protein in a catalytic activity-dependent manner.We further used IP to investigate the potential interaction between YOD1 and p53."

37454155

➶

biogrid

No evidence text available

37454155

➶

sparser

"These results demonstrate that YOD1 interacts with p53 through the N-terminus of p53 and the OTU domain of YOD1."

37454155

➶

reach

"As expected, we detected clear binding of Myc-p53 to Flag-YOD1 (Fig. 3B)."

37454155

➶

reach

"In addition, we detected an interaction and colocalization of endogenous YOD1 and p53 in MV-4–11 cells (Fig. 3C and Supplementary Fig. 4)."

37454155

YOD1 deubiquitinates TP53.

| 3

YOD1 leads to the deubiquitination of TP53. 3 / 3

| 3

➶

reach

"As expected, the knockdown of YOD1 increased p53 ubiquitination, while overexpression of the mutant showed no effect (Fig. 3E, F and Supplementary Fig. 5B), indicating that the protein level of catalytically active YOD1 is responsible for the changes in p53 ubiquitination."

37454155

➶

reach

"Interestingly, we found that YOD1 transfection inhibited the p53-MDM2 interaction (Fig. 3I) and consequently MDM2-induced p53 ubiquitination (Fig. 3J)."

37454155

➶

reach

"Presumably, this may lead to the deubiquitination of p53 by YOD1."

37454155

YOD1 affects MYH9

1 | 8 6

YOD1 binds MYH9.

1 | 3 4

YOD1 binds MYH9. 8 / 8

1 | 3 4

➶

sparser

"Mechanistically, YOD1 binds to MYH9 and maintains its stability by removing the K48 ubiquitin chain from MYH9, thereby mediating the microglia polarization signaling pathway to mediate microglia homeostasis."

40041897

➶

reach

"By co-transfecting MYH9 and mutated YOD1 plasmids in NIH/3T3 cells, it was determined that when amino acids 313 to 317 are deleted, YOD1 failed to bind MYH9, while YOD1 with mutations in other domains can still bind to MYH9 and function (Fig. 7H).3.8 YOD1 regulates the deubiquitination of MYH9."

40041897

➶

sparser

"β -Amyloid stimulated the expression of YOD1, causing the binding of YOD1 to MYH9."

40041897

➶

sparser

"Further, Co-IP verification found that the interaction between YOD1 and MYH9 was significantly increased in brain tissue ( xref D), microglia cells ( xref E), and NIH/3T3 cells ( xref F)."

40041897

➶

biogrid

No evidence text available

37667382

➶

sparser

"In this study, we showed that YOD1 can bind to MYH9 via its C2H2-type domain."

40041897

➶

reach

40041897

➶

reach

"Further, Co-IP verification found that the interaction between YOD1 and MYH9 was significantly increased in brain tissue (Fig. 7D), microglia cells (Fig. 7E), and NIH/3T3 cells (Fig. 7F)."

40041897

YOD1 deubiquitinates MYH9.

| 4

YOD1 deubiquitinates MYH9. 4 / 4

| 4

➶

reach

"These results indicate that histidine at position H262 of YOD1 is involved in removing ubiquitin molecules from MYH9, thereby preventing its degradation.3.9 YOD1 regulates microglial function through deubiquitinating MYH9."

40041897

➶

reach

"We demonstrate that YOD1 deubiquitinates MYH9, enhances MYH9 stability, and regulates microglial migration, phagocytosis, and inflammation regulation functions."

40041897

➶

reach

"YOD1 regulates microglial homeostasis by deubiquitinating MYH9 to promote the pathogenesis of Alzheimer's disease."

40041897

➶

reach

"The results showed that YOD1 can reduce the ubiquitination of MYH9 (Fig. 7I)."

40041897

YOD1 ubiquitinates MYH9.

| 1 2

YOD1 ubiquitinates MYH9. 3 / 3

| 1 2

➶

sparser

"In NIH/3T3 cells, Co-IP was used to confirm that the central OTU region of MYH9 affects the ubiquitination of MYH9 by YOD1 ( xref K)."

40041897

➶

sparser

"Next, we further explored the regulatory mechanism of MYH9 ubiquitination by YOD1."

40041897

➶

reach

"In NIH/3T3 cells, Co-IP was used to confirm that the central OTU region of MYH9 affects the ubiquitination of MYH9 by YOD1 (Fig. 7K)."

40041897

VCP affects YOD1

11 | 4

VCP binds YOD1.

11 | 3

YOD1 binds VCP. 10 / 14

11 | 3

➶

biogrid

No evidence text available

24417208

➶

biogrid

No evidence text available

24417208

➶

reach

"OTUD2 orthologues bind to the AAA+ ATPase/unfoldase p97/VCP and furnish it with ubiquitin linkage processing or editing capability (Rumpf & Jentsch, 2006; Messick et al, 2008; Ernst et al, 2009)."

36958824

➶

biogrid

No evidence text available

28244869

➶

biogrid

No evidence text available

27753622

➶

biogrid

No evidence text available

19818707

➶

biogrid

No evidence text available

37667382

➶

reach

36170815

➶

biogrid

No evidence text available

27753622

➶

biogrid

No evidence text available

32296183

VCP activates YOD1.

| 1

VCP bound to GEMIN4 activates YOD1. 1 / 1

| 1

➶

reach

"VCP and p97 recruitment to lysosome membranes and functioning are mediated by its cofactors and adaptors YOD1, UBXD1, and PLAA."

32792938

USP25 affects YOD1

| 8 7

YOD1 binds USP25. 10 / 15

| 8 7

➶

sparser

"YOD1 binds to USP21."

37743467

➶

reach

"YOD1 binds to USP21."

37743467

➶

reach

"Subsequently, we investigated the interaction between YOD1 and USP21, and our findings demonstrate that YOD1 indeed binds to USP21 (Fig. 1B, C)."

37743467

➶

reach

"After identifying the binding between YOD1 and USP21, we aimed to investigate which protein acts as a DUB on the other."

37743467

➶

sparser

"Subsequently, we investigated the interaction between YOD1 and USP21, and our findings demonstrate that YOD1 indeed binds to USP21 (Fig. xref B, C)."

37743467

➶

sparser

37743467

➶

reach

37743467

➶

reach

"Our study also sheds light on the interaction between YOD1 and USP21, demonstrating the possibility of deubiquitination of one DUB by another DUB."

37743467

➶

reach

"In summary, our study revealed several notable interactions between YOD1 and USP21."

37743467

➶

sparser

"Our study also sheds light on the interaction between YOD1 and USP21, demonstrating the possibility of deubiquitination of one DUB by another DUB."

37743467

SYVN1 affects YOD1

1 | 6 6

YOD1 binds SYVN1. 10 / 14

1 | 6 6

➶

sparser

"Our coimmunoprecipitation experiments revealed that YOD1 binds to Hrd1 via Hrd1’s cytosolic domain."

24068323

➶

reach

"YOD1 can bind to HRD1, and HRD1 as an ER associated E3 is crucial in the process of reverse transport."

33546413

➶

sparser

"Whether the YOD1-Hrd1 interaction reflects a direct interaction or an interaction mediated by Hrd1 adaptors is unknown."

24068323

➶

reach

"Consistent with this idea, a deubiquitinase called YOD1 binds to Hrd1 and negatively regulates CTA1 retro translocation."

26362261

➶

sparser

"WT Hrd1-Myc coprecipitated with FLAG-WT YOD1 only when FLAG-WT YOD1was expressed in cells ( xref , top and second panels, compare lane 2 with lane 1), indicating that Hrd1 binds to YOD1."

24068323

➶

sparser

24068323

➶

sparser

"This finding indicates that gp78 does not engage YOD1, and demonstrates specificity of the YOD1-Hrd1 interaction."

24068323

➶

biogrid

No evidence text available

24068323

➶

sparser

24068323

➶

reach

"We first tested a potential interaction between YOD1 and Hrd1."

24068323

ZNF24 affects YOD1

| 5 8

YOD1 binds ZNF24. 10 / 13

| 5 8

➶

sparser

40274778

➶

sparser

40274778

➶

sparser

"Interaction of the SCAN box domain of ZNF24 with the UBX-like domain of YOD1."

40274778

➶

sparser

"To gain further insight into the regions necessary for the binding of YOD1 to ZNF24, we generated truncations of YOD1 (Fig. xref ) and ZNF24 (Fig. xref )."

40274778

➶

sparser

"Our study demonstrated for the first time that YOD1 interacted with and stabilized ZNF24, with the UBX-like domain of YOD1 binding to the SCAN box domain of ZNF24."

40274778

➶

sparser

"Coimmunoprecipitation assays demonstrated that both endogenous and exogenous YOD1 interact with the N-terminal SCAN box region of ZNF24 (Fig. xref )."

40274778

➶

reach

"Mechanistically, YOD1 binds directly to and stabilizes ZNF24, specifically removing the K27/29/48-linked ubiquitination of ZNF24 at the K308 site."

40274778

➶

reach

40274778

➶

reach

"In addition, an in vitro Glutathione S-transferase (GST) pull-down assay revealed that the purified protein HIS-YOD1 directly bound to GST-ZNF24 but not to the GST control (Fig. 3H), thereby verifying a direct interaction between YOD1 and ZNF24."

40274778

➶

reach

"To gain further insight into the regions necessary for the binding of YOD1 to ZNF24, we generated truncations of YOD1 (Fig. 4A) and ZNF24 (Fig. 4F)."

40274778

YOD1 affects RIPK2

| 9 4

YOD1 binds RIPK2.

| 2 4

YOD1 binds RIPK2. 6 / 6

| 2 4

➶

sparser

"Given that RIPK2 was identified as a YOD1-interacting protein by mass spectrometry (Fig. xref ) and that YOD1 critically regulated RIPK2 stability (Fig. xref ; Appendix Fig. xref ), we assessed the direct interaction between YOD1 and RIPK2 by co-immunoprecipitation and western blot."

39333628

➶

sparser

"The co-IP result showed that the YOD1 mutant lacking the UBXL domain failed to interact with RIPK2, showing that YOD1 binds RIPK2 through the UBXL domain (Fig. xref )."

39333628

➶

sparser

"In this study, we demonstrate that YOD1 interacts with RIPK2 through the UBXL domain."

39333628

➶

sparser

"Recent research indicates that YOD1 binds to RIPK2 via its UBXL domain and increases the abundance of RIPK2 by reducing K48 polyubiquitination and proteasomal degradation of RIPK2, thereby enhancing NOD2 signaling and exerting a protective role in promoting physiological inflammatory responses in macrophages [ xref ]."

40555982

➶

reach

"YOD1 binds and K48 deubiquitinates RIPK2."

39333628

➶

reach

"Given that RIPK2 was identified as a YOD1-interacting protein by mass spectrometry (Fig. 5B) and that YOD1 critically regulated RIPK2 stability (Fig. 5D–H; Appendix Fig. S12), we assessed the direct interaction between YOD1 and RIPK2 by co-immunoprecipitation and western blot."

39333628

YOD1 activates RIPK2.

| 4

YOD1 activates RIPK2. 4 / 4

| 4

➶

reach

"Indeed, we found that, after blocking protein synthesis with CHX, YOD1 deletion accelerated the degradation of RIPK2 in BMDMs in the presence or absence of L18-MDP (Fig. 5G; Appendix Fig. S12A), indicating that YOD1 increases the stability of RIPK2 protein."

39333628

➶

reach

"Consistently, YOD1 overexpression increased RIPK2 protein abundance and delayed RIPK2 degradation upon CHX treatment (Appendix Fig. S12B)."

39333628

➶

reach

"Since RIPK2 is essential for NOD2 signaling and YOD1 stabilizes RIPK2, overexpression of YOD1 strongly increased L18-MDP-induced NF-κB and MAPK activation as well as cytokine production (Appendix Fig. S13)."

39333628

➶

reach

"Altogether, these findings show that YOD1 enhances NOD2 signaling in macrophages by stabilizing RIPK2."

39333628

YOD1 inhibits RIPK2.

| 3

YOD1 inhibits RIPK2. 3 / 3

| 3

➶

reach

"Consistently, YOD1 overexpression increased RIPK2 protein abundance and delayed RIPK2 degradation upon CHX treatment (Appendix Fig. S12B)."

39333628

➶

reach

"In the present study, we found that NOD2-mediated protective responses in colitis were potentiated by YOD1, which inhibited the proteasomal degradation of RIPK2 by removing K48-linked polyubiquitin chains."

39333628

➶

reach

39333628

YOD1 affects Neoplasm Metastasis

| 13

YOD1 activates Neoplasm Metastasis.

| 7

YOD1 activates Neoplasm Metastasis. 7 / 7

| 7

➶

reach

"In addition, YOD1 deubiquitinase is highly expressed in PDAC and accelerates tumor metastasis, although the underlying mechanism remains unclear (Zhang et al., 2022)."

38174069

➶

reach

"YOD1 knockdown MDA-MB-231 cells were injected into nude mice through the tail vein and the bioluminescent imaging showed YOD1 knockdown could significantly decrease the tumor burden and metastasis."

37667382

➶

reach

"To investigate whether low YOD1 expression promotes ccRCC progression and metastasis via the ZNF24/VEGFA axis, we first cotransfected siYOD1 or Myc-ZNF24 plasmids into A498 and 786-O cells."

40274778

➶

reach

"Conversely, increased YOD1 expression blocks ERK signaling by stabilizing TRIM33 activation, thereby inhibiting tumor invasion and lymph node metastasis in squamous cell carcinoma of the head and neck [25]."

40274778

➶

reach

"YOD1 downregulation promoted ccRCC growth and metastasis both in vitro and in vivo."

40274778

➶

reach

"Thus, DEX activates the miR-143-3p/epidermal growth factor receptor (EGFR) pathway substrate 8 axis, increases miR-493-5p, which targets RASL11B implicated in cancer development, decreases the expression of miR-1307, upregulates miR-185, which inactivates the Sry (sex determining region Y)-box 9 (SOX9)/Wnt/B-catenin pathway and miR-520a-3p that targets the metastases-inducing gene Yod1 coding for the deubiquitinating enzyme YOD1."

38481729

➶

reach

"YOD1 promotes TNBC growth and metastasis in vivo."

37667382

YOD1 inhibits Neoplasm Metastasis.

| 6

YOD1 inhibits Neoplasm Metastasis. 6 / 6

| 6

➶

reach

"Moreover, YOD1 knockdown promoted tumor growth and metastasis in vitro and in vivo."

40274778

➶

reach

"The results revealed that YOD1 knockdown markedly enhanced the growth of subcutaneous tumors (Fig. 1J–L) and the incidence of lung metastasis (Fig. 1M, N) in nude mice and NCG mice."

40274778

➶

reach

"YOD1 inhibits ccRCC cell proliferation and metastasis."

40274778

➶

reach

"Knockdown of YOD1 markedly promoted subcutaneous tumor growth (Fig. 7H–J) and lung metastasis (Fig. 7K, L), and these effects were reversed by ZNF24 overexpression."

40274778

➶

reach

"OTUD1 is negatively regulated by miR-4429 in ovarian cancer, and the expression of YOD1 increases when miR-4429 is reduced, promoting the growth and metastasis of ovarian cancer."

35642058

➶

reach

"Overexpression of YOD1 attenuates tumor growth and lymph node metastasis of HNSCC cells in vivo."

37573347

TP53 affects YOD1

2 | 6 5

TP53 binds YOD1.

2 | 5 5

YOD1 binds TP53. 10 / 12

2 | 5 5

➶

sparser

"We then confirmed that YOD1 interacts with p53 and stabilizes p53 via deubiquitination."

37454155

➶

sparser

"We further used IP to investigate the potential interaction between YOD1 and p53."

37454155

➶

sparser

"Taken together, these data indicated a specific interaction between YOD1 and p53."

37454155

➶

sparser

37454155

➶

reach

"We then confirmed that YOD1 interacts with p53 and stabilizes p53 via deubiquitination."

37454155

➶

reach

"These results indicate that YOD1 stabilizes the p53 protein in a catalytic activity-dependent manner.We further used IP to investigate the potential interaction between YOD1 and p53."

37454155

➶

biogrid

No evidence text available

37454155

➶

sparser

"These results demonstrate that YOD1 interacts with p53 through the N-terminus of p53 and the OTU domain of YOD1."

37454155

➶

reach

"As expected, we detected clear binding of Myc-p53 to Flag-YOD1 (Fig. 3B)."

37454155

➶

reach

"In addition, we detected an interaction and colocalization of endogenous YOD1 and p53 in MV-4–11 cells (Fig. 3C and Supplementary Fig. 4)."

37454155

TP53 activates YOD1.

| 1

TP53 activates YOD1. 1 / 1

| 1

➶

reach

"Together, these data indicate that miR-221/222 enhances the ubiquitination of p53 and causes its degradation by targeting YOD1."

37454155

YOD1 affects MAVS

3 | 7 2

YOD1 inhibits MAVS.

| 6

YOD1 inhibits MAVS. 6 / 6

| 6

➶

reach

"The deubiquitinase YOD1 also antagonizes MAVS aggregation by cleaving K63-linked poly-Ub chains on MAVS [57]."

39193925

➶

reach

"Subsequently, YOD1 removes the K63 linked ubiquitin chain and abrogates the aggregation of MAVS, thus inhibiting not only IRF3 and p65 activation, but also IFN-beta production."

33511009

➶

reach

"Our previous studies have demonstrated that YOD1 negatively regulated RIG-I-mitochondrial antiviral signaling protein (MAVS) signaling during RNA virus infection."

38789414

➶

reach

"YOD1 suppressed MAVS aggregation through cleaving the K63-linked ubiquitination, which led to reduced IFN-β production and cellular antiviral response [34]."

38789414

➶

reach

"The Otubain YOD1 Suppresses Aggregation and Activation of the Signaling Adaptor MAVS through Lys63 Linked Deubiquitination."

30952814

➶

reach

"Distinguishing from the above E3s, YOD1 suppress MAVS aggregation through Lys63-linked deubiquitination (97)."

37901251

YOD1 binds MAVS.

3 | 2

MAVS binds YOD1. 5 / 5

3 | 2

➶

sparser

"YOD1 interacts with MAVS through its ubiquitin regulatory X and ZF domains and removes K63-linked polyubiquitin chains on MAVS, thereby inhibiting the formation of MAVS prion-like aggregates ( xref )."

36505476

➶

sparser

"After viral infection, OTUD2 (also known as YOD1) interacts with MAVS through its UBX and Znf domains at the mitochondria, followed by the cleavage the K63-linked ubiquitin chains of MAVS that induced the abrogation of MAVS oligomerization to attenuate the IFN production [ xref ]."

35008917

➶

biogrid

No evidence text available

30952814

➶

biogrid

No evidence text available

30952814

➶

biogrid

No evidence text available

30952814

YOD1 activates MAVS.

| 1

YOD1 activates MAVS. 1 / 1

| 1

➶

reach

"Liu and colleagues reported YOD1 mediated K63 linked deubiquitination could activate an innate antiviral immune response against viral infection, and the aggregation of MAVS."

33343629

YOD1 is modified

| 12

YOD1 is ubiquitinated.

| 10

YOD1 is ubiquitinated. 10 / 10

| 10

➶

sparser

"In this study, we observed TRIM54 binds and increases ubiquitination of YOD1 ( xref )."

38042492

➶

sparser

"The ubiquitination of YOD1 at lysine 213/257 is a novel observation that is not reported previously."

36182100

➶

sparser

"Further, through immunoprecipitation studies, we identified that TRIM54 regulates inflammation in TDSCs by binding to and ubiquitinating YOD1."

38042492

➶

sparser

"Both YOD1 and USP21 are ubiquitinated."

37743467

➶

sparser

"Since TRIM54 is an ubiquitinating enzyme, we wanted to assess if it binds and increases the ubiquitination of YOD1."

38042492

➶

sparser

"Initially, we overexpressed TRIM54 and observed an increase in ubiquitination of endogenous YOD1 protein in HEK 293T cells ( xref A )."

38042492

➶

sparser

"Initially, we observed that indeed ubiquitination of YOD1 increases in the presence of TRIM54, however presence of deubiquitinase 3 significantly decreased this ubiquitination ( xref C )."

38042492

➶

| 3

YOD1 deubiquitinates PML. 2 / 2

| 2

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reach

"Taken together, these results strongly indicate that YOD1 regulates the stability of both the wild-type and mutant forms of PML/RARα.3.3 YOD1 deubiquitinates wild-type PML/RARalpha and its drug-resistant mutants."

35847510

➶

reach

"Results reveal that purified YOD1 effectively deubiquitinated PML/RARα in vitro, while YOD1(C160S) failed to deubiquitinate PML/RARα (Fig. 3F), confirming the importance of YOD1 catalytic activity to PML/RARα deubiquitination."

35847510

YOD1-C160S deubiquitinates PML. 1 / 1

| 1

➶

reach

35847510

YOD1 activates PML.

| 3

YOD1 activates PML. 3 / 3

| 3

➶

reach

"Blockade of deubiquitinase YOD1 degrades oncogenic PML/RARalpha and eradicates acute promyelocytic leukemia cells."

35847510

➶

reach

"3.1 YOD1 is identified as a key DUB to modulate the stability of the PML/RARalpha protein."

35847510

➶

reach

"Suppression of YOD1 promoted the degradation of PML/RARalpha, thus inhibiting APL cells and prolonging the survival time of APL cell-bearing mice."

35847510

YOD1 decreases the amount of PML.

| 2

YOD1 decreases the amount of PML. 2 / 2

| 2

➶

reach

"In line with these results, YOD1 also notably downregulated the ubiquitination levels of drug-resistant PML/RARα mutants (ΔF286, R276Q, L218P and A216V) (Fig. 3E), strongly supporting that YOD1 may deubiquitination of PML/RARα."

35847510

➶

reach

35847510

YOD1 affects Neoplasms

| 11

YOD1 inhibits Neoplasms.

| 9

YOD1 inhibits Neoplasms. 9 / 9

| 9

➶

reach

"The results revealed that YOD1 knockdown markedly enhanced the growth of subcutaneous tumors (Fig. 1J–L) and the incidence of lung metastasis (Fig. 1M, N) in nude mice and NCG mice."

40274778

➶

reach

"Notably, YOD1 knockdown significantly promoted the proliferation of tumor organoids derived from ccRCC patients (Fig. 2G)."

40274778

➶

reach

40274778

➶

reach

"In another study, overexpression of YOD1 abrogated miR-373-induced cervical cancer progression, including cell proliferation, colony formation in vitro as well as tumor growth assays in vivo [ 17 ]."

33515913

➶

reach

"Knockdown of YOD1 markedly promoted subcutaneous tumor growth (Fig. 7H–J) and lung metastasis (Fig. 7K, L), and these effects were reversed by ZNF24 overexpression."

40274778

➶

reach

"Moreover, YOD1 knockdown promoted tumor growth and metastasis in vitro and in vivo."

40274778

➶

reach

"Overexpression of YOD1 attenuates tumor growth and lymph node metastasis of HNSCC cells in vivo."

37573347

➶

reach

"Deubiquitinase YOD1 suppresses tumor progression by stabilizing E3 ligase TRIM33 in head and neck squamous cell carcinoma."

37573347

➶

reach

"The deubiquitinase YOD1 suppresses tumor progression by stabilizing ZNF24 in clear cell renal carcinoma."

40274778

YOD1 activates Neoplasms.

| 2

YOD1 activates Neoplasms. 2 / 2

| 2

➶

reach

"YOD1 knockdown MDA-MB-231 cells were injected into nude mice through the tail vein and the bioluminescent imaging showed YOD1 knockdown could significantly decrease the tumor burden and metastasis."

37667382

➶

reach

"In addition, YOD1 deubiquitinase is highly expressed in PDAC and accelerates tumor metastasis, although the underlying mechanism remains unclear (Zhang et al., 2022)."

38174069

YOD1 affects NFkappaB

| 7

YOD1 activates NFkappaB.

| 4

YOD1 activates NFkappaB. 4 / 7

| 4

➶

reach

"However, why and how YOD1 promotes canonical NF-kappaB signaling and activation in the context of p62 independent CD40 or RANK stimulation is currently unclear and we can only speculate."

28244869

➶

reach

"Potentially, YOD1 could also function as a p97 co-factor for IkappaBalpha degradation to support canonical NF-kappaB signaling, but it is unclear why this would affect signaling in response to some inducers (e.g. CD40) while others are unaffected (e.g. IL-1 and TNF)."

28244869

➶

| 11

➶

sparser

"To investigate whether the α-synuclein protein stability is mediated by YOD1, Myc-YOD1 was transfected in a dose-dependent manner."

36682332

➶

sparser

"In this study, we investigated the ubiquitination of Myc-YOD1 and Flag-USP21 using wild-type HA- Ub and various lysine mutant forms of it."

37743467

➶

sparser

"To evaluate the effect of YOD1 on the stability of USP21, we transfected HEK293T, HeLa, and A549 cell lines with increasing doses of Myc-YOD1 and assessed the expression level of exogenous USP21."

37743467

➶

sparser

"Additionally, we examined which lysine-linked polyubiquitin chain is associated with the proteasomal degradation of Myc-YOD1."

37743467

➶

sparser

"Subsequently, we transfected increasing doses of Flag- USP21 into HEK293T, HeLa, and A549 cells to evaluate the stability change of Myc-YOD1."

37743467

➶

sparser

"Surprisingly, each lysine mutant form of HA-Ub was capable of forming polyubiquitin chains on Myc-YOD1, and each type of lysine mutant form-linked polyubiquitin chain on Myc-YOD1 was related to proteasomal degradation (Fig. xref C)."

37743467

➶

sparser

"Interestingly, we found that the expression level of Myc-YOD1 remained unchanged in HEK293T cells exposed to Flag- USP21 (Fig. xref C), and there was no significant difference in YOD1 stability observed in HeLa and A549 cells (Fig. xref D)."

37743467

➶

sparser

"For deubiquitination assay, Flag-α-synuclein, HA-ubiquitin-WT, its lysine mutants (HA-Ub + K6 , HA-Ub + K11 , HA-Ub + K27 , HA-Ub + K29 , HA-Ub + K33 , HA-Ub + K48 , and HA-Ub + K63 ), and Myc-YOD1 we[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

36682332

➶

sparser

"We observed that the expression level of NEDD4 was significantly increased, and α-synuclein was decreased in a dose-dependent manner of Myc-YOD1 ( Fig. 4 A and B)."

36682332

➶

sparser

"In the deubiquitination assay of Myc-YOD1, the formation of a polyubiquitin chain on Myc-YOD1 was reduced by Flag-USP21 compared to a catalytically inactive mutant of Flag-USP21 (C221S), indicating that USP21 functions as a DUB of YOD1 (Fig. xref A)."

37743467

YOD1 affects GEMIN4

| 11

YOD1 binds GEMIN4.

| 9

YOD1 binds GEMIN4. 7 / 7

| 7

➶

reach

19818707

➶

reach

"The DUB YOD1, which binds to p97 via its UBX (ubiquitin regulatory X) domain, is also associated with ERAD [XREF_BIBR]."

28819009

➶

reach

"We show that a catalytically inactive deubiquitinating enzyme and p97 cofactor YOD1 enhances the accumulation of K11- and K48 linked polyubiquitin in the cytoplasm, at the ER membrane, and bound to p97."

24417208

➶

reach

"In addition, p97 binds to a deubiquitinating enzyme YOD1, presumably so that polyubiquitin chains will not interfere with insertion into the proteasome."

25071743

➶

reach

"In addition, whereas both H. sapiens YOD1 and OTUD2 and S. cerevisiae Otu1 interact with p97 and Cdc48 through an N-terminal UBL or UBX domain, the A. thaliana OTU2 has a relatively shorter N-terminus and does not interact with CDC48."

24659992

➶

reach

"yOtu1 and D.melanogaster (dm) Otu1 are orthologs of human OTUD2 (38% and 53% identical in OTU domain, respectively), and OTUD2 and yOtu1 both bind cdc48 and p97 and are involved in endoplasmic-reticulum-associated protein degradation."

23827681

➶

reach

"To confirm that p97 and YOD1 form a complex in a cellular context, we transfected FLAG tagged YOD1 variants, followed by preparation of detergent extracts."

sparser

"To evaluate the effect of YOD1 on the stability of USP21, we transfected HEK293T, HeLa, and A549 cell lines with increasing doses of Myc-YOD1 and assessed the expression level of exogenous USP21."

37743467

➶

sparser

"Additionally, we examined which lysine-linked polyubiquitin chain is associated with the proteasomal degradation of Myc-YOD1."

37743467

➶

sparser

"Subsequently, we transfected increasing doses of Flag- USP21 into HEK293T, HeLa, and A549 cells to evaluate the stability change of Myc-YOD1."

37743467

➶

sparser

37743467

➶

sparser

37743467

➶

sparser

36682332

➶

sparser

"We observed that the expression level of NEDD4 was significantly increased, and α-synuclein was decreased in a dose-dependent manner of Myc-YOD1 ( Fig. 4 A and B)."

36682332

➶

sparser

37743467

YOD1 affects SNCA

4 | 6

YOD1 binds SNCA. 10 / 10

4 | 6

➶

sparser

"However, since the interaction between YOD1 and α-synuclein has not been elucidated, the current study investigated the relationship between YOD1 and α-synuclein."

36682332

➶

sparser

"We found that YOD1 directly interacts with α-synuclein and deubiquitinates K6-, K11-, K29-, K33-, and K63-linked polyubiquitin chains on α-synuclein."

36682332

➶

sparser

"YOD1 directly interacts with α-synuclein and removes various polyubiquitin chains (K6, K11, K29, K33, and K63) through deubiquitination, thus regulating protein stability and reducing aggregation."

40555982

➶

biogrid

No evidence text available

30021006

➶

sparser

"Next, we performed the GST pull-down assay to check the direct interaction between YOD1 and α-synuclein, and found that GST-YOD1 precipitates α-synuclein ( Fig. 1 B)."

36682332

➶

sparser

"These results indicate the direct interaction between YOD1 and α-synuclein."

36682332

➶

biogrid

No evidence text available

36682332

➶

biogrid

No evidence text available

36682332

➶

biogrid

No evidence text available

36682332

➶

sparser

"Mechanistically, OTUD2 directly interacts with α-synuclein to cleave K6-, K11-,K33-,K63- and K29-linked ubiquitin chains, representing a novel regulatory mechanism (Park et al. xref )."

41204197

YOD1 affects NEDD4

3 | 6 1

YOD1 binds NEDD4.

3 | 1 1

YOD1 binds NEDD4. 5 / 5

3 | 1 1

➶

biogrid

No evidence text available

31916733

➶

biogrid

No evidence text available

31916733

➶

sparser

"The interaction between YOD1 and NEDD4 was uncovered using the PPI network [ xref ]."

37743467

➶

reach

"The interaction between YOD1 and NEDD4 was uncovered using the PPI network [22]."

37743467

➶

biogrid

No evidence text available

23827681

YOD1 deubiquitinates NEDD4.

| 4

YOD1 deubiquitinates NEDD4. 4 / 4

| 4

➶

reach

"YOD1 Deubiquitinates NEDD4 Involved in the Hippo Signaling Pathway."

31916733

➶

reach

"In a previous study, we demonstrated that YOD1 deubiquitinates and regulates the cellular function of NEDD4 (an E3 ligase) that degrades α-synuclein [ 35 ]."

36682332

➶

reach

"Moreover, YOD1 is responsible for deubiquitinating NEDD4 and inhibiting NEDD4-mediated proliferation via the Hippo signaling pathway [22]."

37743467

➶

reach

"In a previous study, we demonstrated that YOD1 regulates the Hippo signaling pathway by deubiquitinating NEDD4, an E3 ligase of LATS1 [23]."

37743467

YOD1 inhibits NEDD4.

| 1

YOD1 inhibits NEDD4. 1 / 1

| 1

➶

reach

"YOD1 was found to promote the degradation of α-Syn and inhibit PD progression by upregulating Nedd4."

38931449

TRIM54 affects YOD1

1 | 6 3

TRIM54 binds YOD1.

1 | 4 2

TRIM54 binds YOD1. 7 / 7

1 | 4 2

➶

reach

"BioGRID Database Statistics predicted a possible interaction between TRIM54 and YOD1 (https://thebiogrid.org/), hence we wanted to assess YOD1’s role during inflammation."

38042492

➶

reach

"Co-IP studies confirmed that TRIM54 binds and regulates YOD1 ubiquitination (Fig. 5)."

38042492

➶

reach

"In this study, we observed TRIM54 binds and increases ubiquitination of YOD1 (Fig. 6)."

38042492

➶

biogrid

No evidence text available

22626734

➶

sparser

"BioGRID Database Statistics predicted a possible interaction between TRIM54 and YOD1 (), hence we wanted to assess YOD1’s role during inflammation."

38042492

➶

sparser

"Hence, based on these results, it is evident that TRIM54 binds to and ubiquitinates YOD1 protein."

38042492

➶

reach

"TRIM54 interacts and ubiquitinates YOD1."

38042492

TRIM54 ubiquitinates YOD1.

| 2 1

TRIM54 ubiquitinates YOD1. 3 / 3

| 2 1

➶

reach

"Hence, based on these results, it is evident that TRIM54 binds to and ubiquitinates YOD1 protein."

38042492

➶

sparser

"Hence, based on these results, it is evident that TRIM54 binds to and ubiquitinates YOD1 protein."

38042492

➶

reach

"Further, through immunoprecipitation studies, we identified that TRIM54 regulates inflammation in TDSCs by binding to and ubiquitinating YOD1."

38042492

SNCA affects YOD1

4 | 6

YOD1 binds SNCA. 10 / 10

4 | 6

➶

sparser

"However, since the interaction between YOD1 and α-synuclein has not been elucidated, the current study investigated the relationship between YOD1 and α-synuclein."

36682332

➶

sparser

"We found that YOD1 directly interacts with α-synuclein and deubiquitinates K6-, K11-, K29-, K33-, and K63-linked polyubiquitin chains on α-synuclein."

36682332

➶

sparser

"YOD1 directly interacts with α-synuclein and removes various polyubiquitin chains (K6, K11, K29, K33, and K63) through deubiquitination, thus regulating protein stability and reducing aggregation."

40555982

➶

biogrid

No evidence text available

30021006

➶

sparser

"Next, we performed the GST pull-down assay to check the direct interaction between YOD1 and α-synuclein, and found that GST-YOD1 precipitates α-synuclein ( Fig. 1 B)."

36682332

➶

sparser

"These results indicate the direct interaction between YOD1 and α-synuclein."

36682332

➶

biogrid

No evidence text available

36682332

➶

biogrid

No evidence text available

36682332

➶

biogrid

No evidence text available

36682332

➶

sparser

"Mechanistically, OTUD2 directly interacts with α-synuclein to cleave K6-, K11-,K33-,K63- and K29-linked ubiquitin chains, representing a novel regulatory mechanism (Park et al. xref )."

41204197

MIR21 affects YOD1

| 9 1

MIR21 inhibits YOD1.

| 7 1

MIR21 inhibits YOD1. 8 / 8

| 7 1

➶

reach

"These findings indicate that miR-21 inhibited YOD1 at the translational level, but did not induce degradation of YOD1 mRNA."

37568787

➶

reach

"zTo confirm that the YOD1 suppression by miR-21 leads to the degradation of desmin during CVB3 infection, we utilized small interference RNA (siRNA) to knockdown endogenous YOD1, which mimicked the effect of miR-21 on YOD1 expression."

24722419

➶

reach

"To further confirm that YOD1 inhibition and desmin downregulation is mediated by miR-21, we transfected cells with miR-362 as an additional non specific control, which showed no effect on the expression levels of gamma-catenin, desmin, YOD1 and ubiquitinated desmin."

24722419

➶

reach

"Our results indicated that miR-21 negatively regulates YOD1 by binding to two sites in its 3′-UTR region."

37230199

➶

reach

"Accordingly, miR-21 knockdown increased YOD1 and decreased α-SMA in CAFs ( Fig. 5 E, H)."

37230199

➶

reach

"Suppression of YOD1 by miR-21 promoted desmin degradation and desmosome disorganization."

24722419

➶

reach

"Similar reduction of YOD1 by miR-21 was observed in CVB3 infected samples (XREF_FIG)."

24722419

➶

sparser

"These findings indicate that miR-21 inhibited YOD1 at the translational level, but did not induce degradation of YOD1 mRNA."

37568787

MIR21 activates YOD1.

| 2

MIR21 activates YOD1. 2 / 2

| 2

➶

reach

"In coxsackie virus B3 (CVB3)-infected myocarditis, the expression of miR-21 that targeted the mRNA of the deubiquitinase YOD1 was up-regulated, resulting in the downregulation of YOD1."

38525836

➶

reach

"Furthermore, infection by the enterovirus coxsackievirus B3 (CVB3) leads to cardiac failure by inducing miR-21 expression, which targets the deubiquitinating enzyme YOD1."

25795143

GEMIN4 affects YOD1

| 10

GEMIN4 binds YOD1.

| 9

YOD1 binds GEMIN4. 7 / 7

| 7

➶

reach

19818707

➶

reach

"The DUB YOD1, which binds to p97 via its UBX (ubiquitin regulatory X) domain, is also associated with ERAD [XREF_BIBR]."

28819009

➶

reach

24417208

➶

reach

"In addition, p97 binds to a deubiquitinating enzyme YOD1, presumably so that polyubiquitin chains will not interfere with insertion into the proteasome."

25071743

➶

reach

24659992

➶

reach

23827681

➶

reach

"To confirm that p97 and YOD1 form a complex in a cellular context, we transfected FLAG tagged YOD1 variants, followed by preparation of detergent extracts."

19818707

YOD1 binds GEMIN4 and UBX domain. 2 / 2

| 2

➶

reach

"YOD1 interacts with p97 via its UBX domain."

21468305

➶

reach

"YOD1, the mammalian homolog of Otu1, also interacts with p97 through its UBX domain [XREF_BIBR]."

28159894

GEMIN4 activates YOD1.

| 1

GEMIN4 activates YOD1. 1 / 1

| 1

➶

reach

"VCP and p97 recruitment to lysosome membranes and functioning are mediated by its cofactors and adaptors YOD1, UBXD1, and PLAA."

32792938

YOD1 affects UBC

9 |

YOD1 binds UBC. 9 / 9

9 |

➶

biogrid

No evidence text available

23827681

➶

biogrid

No evidence text available

27753622

➶

biogrid

No evidence text available

19818707

➶

biogrid

No evidence text available

19818707

➶

biogrid

No evidence text available

38803224

➶

biogrid

No evidence text available

23827681

➶

biogrid

No evidence text available

27066941

➶

biogrid

No evidence text available

32296183

➶

biogrid

No evidence text available

40634336

YOD1 affects CTNNB1

1 | 5 3

YOD1 binds CTNNB1.

1 | 1 3

YOD1 binds CTNNB1. 5 / 5

1 | 1 3

➶

reach

"We then conducted LC-MS/MS and co-immunoprecipitation (Co-IP) analyses to verify the binding between YOD1 and EndMT-related proteins, and found that YOD1 directly bound to β-catenin in HUVECs via its ovarian tumor-associated protease (OTU) domain, and histidine at 262 performing deubiquitination to maintain β-catenin protein stability by removing the K48 ubiquitin chain from β-catenin and preventing its proteasome degradation, thereby promoting EndMT of vascular endothelial cells."

38641745

➶

sparser

"YOD1 interacts with β-catenin via its OTU-binding domain, regulating endothelial-mesenchymal transition and vascular remodeling."

40555982

➶

biogrid

No evidence text available

30323974

➶

sparser

38641745

➶

sparser

"In conclusion, we demonstrate a new YOD1-β-catenin axis in regulating Ang II-induced vascular endothelial injury and reveal YOD1 as a deubiquitinating enzyme for β-catenin, suggesting that targeting YOD1 holds promise as a potential therapeutic strategy for treating β-catenin-mediated vascular diseases."

38641745

YOD1 inhibits CTNNB1.

| 4

YOD1 inhibits CTNNB1. 4 / 4

| 4

➶

reach

"YOD1 stabilizes the E3 ligase TRIM33 and inhibits the ERK/beta-catenin signaling pathway."

37573347

➶

reach

"YOD1 inactivated the YAP/beta-catenin pathway."

34282135

➶

reach

"YOD1 inhibited the YAP/beta-catenin pathway."

34282135

➶

reach

"Using the western blot, we found that YOD1 inhibited the activation of ERK and β-catenin signaling pathways in HNSCC cell lines (Fig. 4J and Supplementary Fig. 3)."

37573347

UBC affects YOD1

9 |

YOD1 binds UBC. 9 / 9

9 |

➶

biogrid

No evidence text available

23827681

➶

biogrid

No evidence text available

27753622

➶

biogrid

No evidence text available

19818707

➶

biogrid

No evidence text available

19818707

➶

biogrid

No evidence text available

38803224

➶

biogrid

No evidence text available

23827681

➶

biogrid

No evidence text available

27066941

➶

biogrid

No evidence text available

32296183

➶

biogrid

No evidence text available

40634336

MYH9 affects YOD1

1 | 4 4

MYH9 binds YOD1.

1 | 3 4

YOD1 binds MYH9. 8 / 8

1 | 3 4

➶

sparser

40041897

➶

reach

40041897

➶

sparser

"β -Amyloid stimulated the expression of YOD1, causing the binding of YOD1 to MYH9."

40041897

➶

sparser

"Further, Co-IP verification found that the interaction between YOD1 and MYH9 was significantly increased in brain tissue ( xref D), microglia cells ( xref E), and NIH/3T3 cells ( xref F)."

40041897

➶

biogrid

No evidence text available

37667382

➶

sparser

"In this study, we showed that YOD1 can bind to MYH9 via its C2H2-type domain."

40041897

➶

reach

40041897

➶

reach

"Further, Co-IP verification found that the interaction between YOD1 and MYH9 was significantly increased in brain tissue (Fig. 7D), microglia cells (Fig. 7E), and NIH/3T3 cells (Fig. 7F)."

40041897

MYH9 activates YOD1.

| 1

MYH9 activates YOD1. 1 / 1

| 1

➶

reach

"To further confirm whether MYH9 mediates YOD1's regulation of microglial function."

40041897

YOD1 affects TRIM33

| 6 2

YOD1 binds TRIM33.

| 2 2

TRIM33 binds YOD1. 4 / 4

| 2 2

➶

sparser

"Immunoprecipitation and fluorescence co-localization assays were used to verify that YOD1 was directly bound to TRIM33 (Fig. xref )."

37573347

➶

reach

"According to the results of liquid chromatography-mass spectrometry analysis (Fig. 4A), we found that the E3 ligase TRIM33 may bind to YOD1."

37573347

➶

reach

"Immunoprecipitation and fluorescence co-localization assays were used to verify that YOD1 was directly bound to TRIM33 (Fig. 4B, C)."

37573347

➶

sparser

"According to the results of liquid chromatography-mass spectrometry analysis (Fig. xref ), we found that the E3 ligase TRIM33 may bind to YOD1."

37573347

YOD1 inhibits TRIM33.

| 3

YOD1 inhibits TRIM33. 3 / 3

| 3

➶

reach

"Deubiquitinase YOD1 suppresses tumor progression by stabilizing E3 ligase TRIM33 in head and neck squamous cell carcinoma."

37573347

➶

reach

40274778

➶

reach

"Inversely, loss of YOD1 decreased TRIM33 obviously (Fig. 4D)."

37573347

YOD1 decreases the amount of TRIM33.

| 1

YOD1 decreases the amount of TRIM33. 1 / 1

| 1

➶

reach

"As shown in Fig. 4G, the ubiquitination level of TRIM33 was significantly reduced by wild-type YOD1 instead of C160S mutant, indicating that deubiquitinating enzyme activity was required for the effect of YOD1 on TRIM33 (Fig. 4G)."

37573347

YOD1 affects STAT3

| 8

STAT3 binds YOD1. 8 / 8

| 8

➶

sparser

"Inhibition of STAT3 abolished the cardioprotective effects of YOD1CKO in Ang II–infused mice, validating the YOD1-STAT3 axis in regulating cardiac hypertrophy."

40561034

➶

sparser

"YOD1 directly interacts with STAT3."

40561034

➶

sparser

"This study clarifies the role of YOD1 and introduces a previously unidentified YOD1-STAT3 axis in regulating pathological cardiac hypertrophy, providing valuable insights for drug development targeting this condition."

40561034

➶

sparser

"We confirmed the interaction between endogenous YOD1 and STAT3 in Ang II–induced mouse cardiac tissue ( xref )."

40561034

➶

sparser

"Exogenous YOD1 and STAT3 interaction was also observed in NIH 3T3 cells transfected with these two plasmids ( xref )."

40561034

➶

sparser

"Therefore, selecting YOD1 as an intervention on the YOD1-STAT3 axis can specifically inhibit STAT3 in pathological hypertrophic hearts, with less impacts on other tissues or cells."

40561034

➶

sparser

"These outcomes indicate that the YOD1-STAT3 axis plays a significant role in regulating pathological cardiac remodeling induced by Ang II."

40561034

➶

sparser

"To further investigate the specific regions in the YOD1-STAT3 interaction, plasmids containing various YOD1 mutants were constructed ( xref )."

40561034

YOD1 affects SQSTM1

| 4 4

YOD1 binds SQSTM1.

| 1 4

TRAF6 binds YOD1 and SQSTM1. 3 / 3

| 3

➶

sparser

"By directly comparing the binding of YOD1 and p62 to TRAF6 in HEK293 cells we could confirm that both proteins are associating with the C-terminal MATH domain ( xref )."

28244869

➶

sparser

"Also, we quantified the amount of p62 and YOD1 bound to TRAF6 after double transfection (control, set to 1) and triple transfection (TRAF6, p62 and YOD1) in three independent experiments ( xref )."

28244869

➶

sparser

28244869

YOD1 binds SQSTM1. 2 / 2

| 1 1

➶

reach

"By directly comparing the binding of YOD1 and p62 to TRAF6 in HEK293 cells we could confirm that both proteins are associating with the C-terminal MATH domain (XREF_FIG)."

28244869

➶

sparser

"YOD1 competitively binds to p62 and interferes with the interaction between TRAF6 and p62 via a noncatalytic mechanism."

40555982

YOD1 inhibits SQSTM1.

| 3

YOD1 inhibits SQSTM1. 3 / 3

| 3

➶

reach

"Hence, our data define that YOD1 antagonizes TRAF6 and p62 dependent IL-1 signaling to NF-kappaB."

28244869

➶

reach

"YOD1 antagonizes ubiquitin ligase TRAF6 and p62 dependent interleukin-1 signaling to NF-kappaB."

33343629

➶

reach

28244869

YOD1 affects Neoplasm Invasiveness

| 8

YOD1 inhibits Neoplasm Invasiveness.

| 6

YOD1 inhibits Neoplasm Invasiveness. 6 / 6

| 6

➶

reach

"YOD1 inhibits migration and invasion of HNSCC cells in vitro."

37573347

➶

reach

"However, opposite outcomes were observed in the two cell lines after transfection, as YOD1 deficiency obviously accelerated migration and invasion in SCC15 and SCC25 cells (Fig. 3C, D)."

37573347

➶

reach

"All results indicate that YOD1 inhibits the migration and invasion of HNSCC cells."

37573347

➶

reach

"Furthermore, YOD1 knockdown promoted cell migration and invasion, whereas YOD1 overexpression inhibited those processes (Fig. 2H)."

40274778

➶

reach

40274778

➶

reach

"The results of the in vitro and in vivo experiments verified that YOD1 depletion significantly promoted growth, invasion, and epithelial-mesenchymal transition in HNSCC."

37573347

YOD1 activates Neoplasm Invasiveness.

| 2

YOD1 activates Neoplasm Invasiveness. 2 / 2

| 2

➶

reach

"YOD1 overexpression promotes TNBC cell proliferation, migration and invasion."

37667382

➶

reach

"The results showed that YOD1 knockdown suppressed the migration and invasion abilities of BT-549 and MDA-MB-231 cell lines, while YOD1 overexpression markedly promoted the migration and invasiveness in both two cells (Fig. 2E)."

37667382

STAT3 affects YOD1

| 8

STAT3 binds YOD1. 8 / 8

| 8

➶

sparser

"Inhibition of STAT3 abolished the cardioprotective effects of YOD1CKO in Ang II–infused mice, validating the YOD1-STAT3 axis in regulating cardiac hypertrophy."

40561034

➶

sparser

"YOD1 directly interacts with STAT3."

40561034

➶

sparser

40561034

➶

sparser

"We confirmed the interaction between endogenous YOD1 and STAT3 in Ang II–induced mouse cardiac tissue ( xref )."

40561034

➶

sparser

"Exogenous YOD1 and STAT3 interaction was also observed in NIH 3T3 cells transfected with these two plasmids ( xref )."

40561034

➶

sparser

"Therefore, selecting YOD1 as an intervention on the YOD1-STAT3 axis can specifically inhibit STAT3 in pathological hypertrophic hearts, with less impacts on other tissues or cells."

40561034

➶

sparser

"These outcomes indicate that the YOD1-STAT3 axis plays a significant role in regulating pathological cardiac remodeling induced by Ang II."

40561034

➶

sparser

"To further investigate the specific regions in the YOD1-STAT3 interaction, plasmids containing various YOD1 mutants were constructed ( xref )."

40561034

YOD1 affects coagulation

| 7

YOD1 inhibits coagulation.

| 5

YOD1 inhibits coagulation. 5 / 5

| 5

➶

reach

"YOD1 suppressed coagulation primarily via inhibiting NLRP3 inflammasome activation."

38789414

➶

reach

"We hypothesized that YOD1 suppressed coagulation primarily via inhibiting NLRP3 inflammasome activation."

38789414

➶

reach

"Taken together, we confirmed that YOD1 suppressed coagulation primarily via inhibiting NLRP3 inflammasome activation."

38789414

➶

reach

"Deficiency of YOD1 expression enhanced NLRP3 inflammasome activation and coagulation both in vitro and in vivo."

38789414

➶

reach

"Mechanically, upon methicillin-resistant Staphylococcus aureus (MRSA) infection in macrophages, YOD1 interacted with NLRP3 to specifically remove its K33-linked ubiquitination, subsequently inhibiting NLRP3 inflammasome activation and coagulation."

38789414

YOD1 activates coagulation.

| 2

YOD1 activates coagulation. 2 / 2

| 2

➶

reach

"Thus, the activation of NLRP3 inflammasome was limited in a proper intensity and time course to avoid coagulation.Our study highlighted the impact of YOD1 on the regulation of NLRP3 inflammasome-mediated coagulation activation."

38789414

➶

reach

38789414

YOD1 affects cell migration

| 1 6

YOD1 activates cell migration.

| 1 4

YOD1 activates cell migration. 5 / 5

| 1 4

➶

reach

"YOD1 overexpression enhances cell migration by promoting TGF-beta3 signaling which may play an important role in lip and palate formation."

30145984

➶

eidos

"RNA interference and overexpression experiments indicated that YOD1 could enhance cell migration during lip and palate formation through the transforming growth factor ( TGF ) - beta3 signaling pathway ( Zhou et al ., 2018 ) ."

33343629

➶

reach

33343629

➶

reach

"Knockdown of YOD1 effectively inhibited TNBC cell migration, proliferation, cell cycle and resistance to cisplatin and paclitaxel."

37667382

➶

reach

"Inhibition of Cell Migration by YOD1 siRNA."

30345304

YOD1 inhibits cell migration.

| 2

YOD1 inhibits cell migration. 2 / 2

| 2

➶

reach

"Furthermore, YOD1 knockdown promoted cell migration and invasion, whereas YOD1 overexpression inhibited those processes (Fig. 2H)."

40274778

➶

reach

"However, research on YOD1 in renal cancer is scarce, highlighting the need for investigations into its role and potential as a therapeutic target in this type of carcinoma.In this study, we first conducted an unbiased RNAi screen targeting OTU deubiquitinating enzymes, which revealed that YOD1 deficiency significantly enhances cell migration."

40274778

YOD1 affects TRIM54

1 | 4 2

TRIM54 binds YOD1. 7 / 7

1 | 4 2

➶

reach

"BioGRID Database Statistics predicted a possible interaction between TRIM54 and YOD1 (https://thebiogrid.org/), hence we wanted to assess YOD1’s role during inflammation."

38042492

➶

reach

"Co-IP studies confirmed that TRIM54 binds and regulates YOD1 ubiquitination (Fig. 5)."

38042492

➶

reach

"In this study, we observed TRIM54 binds and increases ubiquitination of YOD1 (Fig. 6)."

38042492

➶

biogrid

No evidence text available

22626734

➶

sparser

"BioGRID Database Statistics predicted a possible interaction between TRIM54 and YOD1 (), hence we wanted to assess YOD1’s role during inflammation."

38042492

➶

sparser

"Hence, based on these results, it is evident that TRIM54 binds to and ubiquitinates YOD1 protein."

38042492

➶

reach

"TRIM54 interacts and ubiquitinates YOD1."

38042492

YOD1 affects PKM2

| 7

YOD1 binds PKM2. 7 / 7

| 7

➶

sparser

"We demonstrate that YOD1 directly interacts with PKM2 and mediates its K63‐linked deubiquitination, thereby inhibiting the transition from tetrameric to dimeric PKM2."

40681475

➶

sparser

"YOD1 directly bound PKM2 and reduces its ubiquitination level by removing the K63‐linked ubiquitin chain of PKM2, thereby increasing the tetramer level and reducing the dimer level of PKM2."

40681475

➶

sparser

"YOD1 binds to PKM2 and reduces its ubiquitination level by removing the K63 ubiquitin chain of PKM2, thereby increasing the tetramer level and reducing the dimer level of PKM2."

40681475

➶

sparser

"Together, this study demonstrates the potential of YOD1 as a novel therapeutic target for PD and provides a mechanistic foundation for developing selective inhibitors of the YOD1‐PKM2 signalling axis."

40681475

➶

sparser

"This study reveals a new YOD1-PKM2 axis in cardiomyocytes and identifies YOD1 as a potential target for the treatment of ISO-induced cardiac remodeling and heart failure."

40500343

➶

sparser

"These above results confirmed that PKM2 interacts with YOD1."

40681475

➶

sparser

"This identifies a novel molecular interface regulating the YOD1‐PKM2 axis in PD pathogenesis."

40681475

YOD1 affects NLRP3 inflammasome complex assembly

| 6

YOD1 inhibits NLRP3 inflammasome complex assembly. 6 / 6

| 6

➶

reach

"Deficiency of YOD1 expression enhanced NLRP3 inflammasome activation and coagulation both in vitro and in vivo."

38789414

➶

reach

"Collectively, these data indicated that YOD1 suppressed NLRP3 inflammasome activation in vitro.Next, we aimed to explore the biological effects of YOD1 on NLRP3 inflammasome activation in vivo."

38789414

➶

reach

"These results indicated that YOD1 could attenuate NLRP3 inflammasome activation in vivo."

38789414

➶

reach

"In our study, we showed that YOD1 interacted with NLRP3 and suppressed the NLRP3 inflammasome activation."

38789414

➶

reach

38789414

➶

reach

"These results demonstrated that YOD1 negatively regulated the NLRP3 inflammasome activation in a deubiquitinase activity-dependent manner and explored a novel function of YOD1.In summary, our study revealed one of the essential and original mechanisms by which YOD1 promoted sepsis-induced DIC, and proposed YOD1 as a priming therapeutic target for NLRP3-inflammasome dysregulation-mediated disorders."

38789414

YOD1 affects ITCH

1 | 5

YOD1 deubiquitinates ITCH. 6 / 6

1 | 5

➶

reach

"A recent study reported that YOD1 could deubiquitinate ITCH, and enhanced the stability of ITCH, which subsequently reduced the levels of LATS and increased the YAP/TAZ level."

34315490

➶

reach

"YOD1 deubiquitinated ITCH and enhanced the stability of ITCH (shown in Figure 3)."

37568787

➶

ubibrowser

"Deubiquitinase YOD1 potentiates YAP/TAZ activities through enhancing ITCH stability"

28416659

➶

reach

"Mechanistically, YOD1 deubiquitinates ITCH, an E3 ligase of LATS, and enhances the stability of ITCH, which leads to reduced levels of LATS and a subsequent increase in the YAP and TAZ level."

28502290

➶

reach

"Mechanistically, YOD1 deubiquitinates ITCH, an E3 ligase of LATS, and enhances the stability of ITCH, which leads to reduced levels of LATS and a subsequent increase in the YAP and TAZ level."

28416659

➶

reach

"YOD1 regulates the Hippo signaling pathway by deubiquitinating and stabilizing ITCH (Kim et al, 2017)."

39333628

RIPK2 affects YOD1

| 2 4

YOD1 binds RIPK2. 6 / 6

| 2 4

➶

sparser

39333628

➶

sparser

"The co-IP result showed that the YOD1 mutant lacking the UBXL domain failed to interact with RIPK2, showing that YOD1 binds RIPK2 through the UBXL domain (Fig. xref )."

39333628

➶

sparser

"In this study, we demonstrate that YOD1 interacts with RIPK2 through the UBXL domain."

39333628

➶

sparser

40555982

➶

reach

"YOD1 binds and K48 deubiquitinates RIPK2."

39333628

➶

reach

39333628

YOD1

1 | 3

YOD1 translocates.

| 3

YOD1 translocates to the mitochondrion. 3 / 3

| 3

➶

reach

"Following a Sendai virus infection, YOD1 was recruited to mitochondria and was shown to interact with MAVS."

33511009

➶

reach

"YOD1 was recruited to mitochondria to interact with MAVS through its UBX and Znf domains after viral infection."

30952814

➶

reach

"As a member of the ovarian tumor family, YOD1 is recruited to mitochondria following viral infection."

36505476

YOD1 binds.

1 |

YOD1 binds YOD1. 1 / 2

1 |

➶

hprd

No evidence text available

7644498

YOD1 affects apoptotic process

| 5

YOD1 activates apoptotic process.

| 3

YOD1 activates apoptotic process. 3 / 3

| 3

| 2

YOD1 increases the amount of RAR. 2 / 2

| 2

➶

reach

35847510

➶

reach

"As expected, YOD1 knockdown significantly decreased the level of endogenous PML/RARα in the NB4 cells (Fig. 1D)."

41326673

➶

sparser

"To validate the specific region of YOD1 that interacts with NCOA4, we generated three plasmids containing different segmental mutations of YOD1."

41326673

➶

sparser

"Mechanistically, we demonstrated that YOD1 directly binds to and deubiquitinates NCOA4 via K48-linked chains, subsequently enhancing NCOA4 expression."

41326673

➶

sparser

"As shown in Fig. xref , Flag-YOD1 and HA-NCOA4 were transfected into HPAEpiC cells as indicated; YOD1 and NCOA4 directly bind to each other under LPS stimulation."

41326673

YOD1 affects IL1

| 1 2

YOD1 inhibits IL1.

| 1

YOD1 inhibits IL1. 1 / 3

| 1

➶

reach

"Hence, our data define that YOD1 antagonizes TRAF6 and p62 dependent IL-1 signaling to NF-kappaB."

28244869

YOD1 binds IL1.

| 2

IL1 binds TRAF6 and YOD1. 2 / 2

| 2

➶

sparser

"Indeed, it seemed likely that in this overexpression scenario there was slightly augmented association of TRAF6 and YOD1 WT after short IL-1 stimulation."

28244869

➶

sparser

28244869

YOD1 affects GST

| 5

YOD1 binds GST. 5 / 5

| 5

➶

sparser

"BL21 competent cells were incubated with GST-YOD1 fastened Glutathione Sepharose."

36682332

➶

sparser

"In briefly, GST vector (pGEX-4T-1) and GST-YOD1 were transformed into the BL21 bacterial strain and incubated in Luria-Bertani broth (cat. no."

36682332

➶

| 2 2

➶

sparser

"Immunoprecipitation and fluorescence co-localization assays were used to verify that YOD1 was directly bound to TRIM33 (Fig. xref )."

37573347

➶

reach

"According to the results of liquid chromatography-mass spectrometry analysis (Fig. 4A), we found that the E3 ligase TRIM33 may bind to YOD1."

37573347

➶

reach

"Immunoprecipitation and fluorescence co-localization assays were used to verify that YOD1 was directly bound to TRIM33 (Fig. 4B, C)."

37573347

➶

sparser

"According to the results of liquid chromatography-mass spectrometry analysis (Fig. xref ), we found that the E3 ligase TRIM33 may bind to YOD1."

37573347

TRIM33 activates YOD1.

| 1

TRIM33 activates YOD1. 1 / 1

| 1

➶

reach

"The deletion of TRIM33 also restored the inhibitory effect of YOD1 on the activation of the above pathways to a certain extent (Fig. 5F)."

37573347

SQSTM1 affects YOD1

| 1 4

TRAF6 binds YOD1 and SQSTM1. 3 / 3

| 3

➶

sparser

"By directly comparing the binding of YOD1 and p62 to TRAF6 in HEK293 cells we could confirm that both proteins are associating with the C-terminal MATH domain ( xref )."

28244869

➶

sparser

"Also, we quantified the amount of p62 and YOD1 bound to TRAF6 after double transfection (control, set to 1) and triple transfection (TRAF6, p62 and YOD1) in three independent experiments ( xref )."

28244869

➶

sparser

30952814

➶

biogrid

No evidence text available

30952814

➶

biogrid

No evidence text available

30952814

GST affects YOD1

| 5

YOD1 binds GST. 5 / 5

| 5

➶

sparser

"BL21 competent cells were incubated with GST-YOD1 fastened Glutathione Sepharose."

36682332

➶

sparser

"In briefly, GST vector (pGEX-4T-1) and GST-YOD1 were transformed into the BL21 bacterial strain and incubated in Luria-Bertani broth (cat. no."

36682332

➶

sparser

"Meantime, recombinant GST-YOD1 and HIS-CDK1 proteins were purified, and the GST pulldown results further excluded the direct interaction between YOD1 and CDK1 ( Fig. xref )."

37667382

➶

sparser

"GST pull-down assay was performed essentially as described [ 37 ], except that a GST-YOD1 vector was used instead of a GST-USP7 vector."

36682332

➶

sparser

"Next, we performed the GST pull-down assay to check the direct interaction between YOD1 and α-synuclein, and found that GST-YOD1 precipitates α-synuclein ( Fig. 1 B)."

36682332

FAF2 affects YOD1

2 | 3

YOD1 binds DERL1 and FAF2. 3 / 3

| 3

➶

sparser

"In agreement with a catalytic role of YOD1 in protein dislocation, the catalytically inactive forms of YOD1 retrieved Derlin-1 and UBXD8 more efficiently than the WT counterpart."

19818707

➶

sparser

"Interaction between YOD1 and other components of ERAD such as Derlin-1 and Ubxd8 were also reported."

25013401

➶

sparser

"Immunoprecipitation experiments showed that YOD1 also interacts with the ERAD machinery components such as Derlin-1 and UBXD8."

22812527

YOD1 binds FAF2. 2 / 2

2 |

➶

biogrid

No evidence text available

19818707

➶

biogrid

No evidence text available

37667382

DERL1 affects YOD1

1 | 1 3

YOD1 binds DERL1 and FAF2. 3 / 3

| 3

➶

sparser

"In agreement with a catalytic role of YOD1 in protein dislocation, the catalytically inactive forms of YOD1 retrieved Derlin-1 and UBXD8 more efficiently than the WT counterpart."

19818707

➶

sparser

"Interaction between YOD1 and other components of ERAD such as Derlin-1 and Ubxd8 were also reported."

25013401

➶

sparser

"Immunoprecipitation experiments showed that YOD1 also interacts with the ERAD machinery components such as Derlin-1 and UBXD8."

22812527

YOD1 binds DERL1. 2 / 2

1 | 1

➶

biogrid

No evidence text available

19818707

➶

reach

24068323

CTNNB1 affects YOD1

1 | 1 3

YOD1 binds CTNNB1. 5 / 5

1 | 1 3

➶

reach

38641745

➶

sparser

"YOD1 interacts with β-catenin via its OTU-binding domain, regulating endothelial-mesenchymal transition and vascular remodeling."

40555982

➶

biogrid

No evidence text available

30323974

➶

sparser

38641745

➶

sparser

38641745

Hsa-miR-98-5p affects YOD1

4 |

Hsa-miR-98-5p decreases the amount of YOD1. 4 / 4

4 |

➶

biopax:mirtarbase

No evidence text available

19088304

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

21572407

Bisphenol A affects YOD1

4 |

Bisphenol A decreases the amount of YOD1.

3 |

Bisphenol A decreases the amount of YOD1. 3 / 3

3 |

➶

ctd

No evidence text available

32528016

➶

| 2

YOD1 activates YAP1. 2 / 2

| 2

➶

reach

"Youngeun Kim et al. also proved that YOD1 is a potent activator of YAP in liver cancer [29]."

35642058

➶

reach

"Through stabilizing the E3 ligase of LATS, ubiquitin thioesterase OTU1 (YOD1) is also reported to modulate the Hippo–YAP/TAZ pathway31."

35024322

YOD1 affects USP21

4 |

USP21 binds YOD1. 4 / 4

4 |

➶

biogrid

No evidence text available

37743467

➶

biogrid

No evidence text available

37743467

➶

biogrid

No evidence text available

37743467

➶

biogrid

No evidence text available

22626734

YOD1 affects TRAF2

| 1 3

YOD1 binds TRAF2. 4 / 4

| 1 3

➶

sparser

"This study shows that the deubiquitinating enzyme YOD1 can bind to the MATH domain of TRAF2 and that this binding is competitive with that of the binding of p62/Sequestosome-1 to TRAF6."

28244869

➶

reach

"Since we did not observe an interaction between YOD1 and TRAF2, which is involved in TNFR signaling, we directly compared the effect of YOD1 knock-down on NF-kappaB activation in response to either IL-1beta or TNFalpha stimulation (XREF_FIG)."

28244869

➶

sparser

"Since we did not observe an interaction between YOD1 and TRAF2, which is involved in TNFR signaling ( xref ; xref ), we directly compared the effect of YOD1 knock-down on NF-κB activation in response to either IL-1β or TNFα stimulation ( xref )."

28244869

➶

sparser

"We did not detect YOD1-TRAF2 binding, indicating a selectivity of YOD1 for association with TRAF6."

28244869

YOD1 affects TNBC

| 4

YOD1 activates TNBC. 4 / 4

| 4

➶

reach

"YOD1 promotes TNBC growth and metastasis in vivo."

37667382

➶

reach

"YOD1 promoted TNBC progression in a manner dependent on its catalytic activity through binding with CDK1, leading to de-polyubiquitylation of CDK1."

37667382

➶

reach

"Knockdown of YOD1 effectively inhibited TNBC cell migration, proliferation, cell cycle and resistance to cisplatin and paclitaxel."

37667382

➶

reach

"Mechanistically, YOD1 promoted TNBC progression in a manner dependent on its catalytic activity through binding with CDK1, leading to de-polyubiquitylation of CDK1 and upregulation of CDK1 expression."

37667382

YOD1 affects SLC25A10

| 4

YOD1 activates SLC25A10. 4 / 4

| 4

➶

reach

"However, it remains unknown how YOD1 affects NLRP3 inflammasome-induced DIC."

38789414

➶

reach

"YOD1 protects against MRSA sepsis-induced DIC through Lys33-linked deubiquitination of NLRP3."

38789414

➶

reach

"The results of the in vitro and in vivo experiments verified that YOD1 depletion significantly promoted growth, invasion, and epithelial-mesenchymal transition in HNSCC."

37573347

➶

reach

"Further research emphasized this theory that YOD1 inhibited the progression of HNSCC."

37573347

YOD1 affects FlaG

| 4

FlaG binds YOD1. 4 / 4

| 4

➶

sparser

"It was found that the level of CDK1 ubiquitination was significantly decreased compared with YOD1-T-OTUD and YOD1-T-ZnF mutant, but increased in the full-length FLAG-YOD1 and YOD1-T-UBXL mutant (Fig. xref G)."

37667382

➶

sparser

37667382

➶

4 |

➶

biogrid

No evidence text available

37743467

➶

biogrid

No evidence text available

37743467

➶

biogrid

No evidence text available

37743467

➶

biogrid

No evidence text available

22626734

TRAF2 affects YOD1

| 1 3

YOD1 binds TRAF2. 4 / 4

| 1 3

➶

sparser

"This study shows that the deubiquitinating enzyme YOD1 can bind to the MATH domain of TRAF2 and that this binding is competitive with that of the binding of p62/Sequestosome-1 to TRAF6."

28244869

➶

reach

28244869

➶

sparser

28244869

➶

sparser

"We did not detect YOD1-TRAF2 binding, indicating a selectivity of YOD1 for association with TRAF6."

28244869

MIR1299 affects YOD1

4 |

MIR1299 decreases the amount of YOD1. 4 / 4

4 |

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

27292025

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

21572407

FlaG affects YOD1

| 4

FlaG binds YOD1. 4 / 4

| 4

➶

sparser

37667382

➶

sparser

37667382

➶

sparser

"As WT YOD1 overexpression also significantly reduced the amount of K48-Ub inside blebs ( xref A,B), we expressed WT YOD1-FLAG in WT and TorsinKO cells undergoing heat shock stress ( xref H)."

35563018

➶

sparser

"However, when WT YOD1-FLAG was expressed, this heat shock-specific increase in K48-Ub was significantly reduced ( xref H)."

35563018

Hsa-mir-4500 affects YOD1

3 |

Hsa-mir-4500 decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-mir-4458 affects YOD1

3 |

Hsa-mir-4458 decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

Hsa-mir-4316 affects YOD1

3 |

Hsa-mir-4316 decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

21572407

Hsa-mir-3658 affects YOD1

3 |

Hsa-mir-3658 decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-miR-93-5p affects YOD1

3 |

Hsa-miR-93-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

22473208

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

22012620

Hsa-miR-888-5p affects YOD1

3 |

Hsa-miR-888-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

21572407

Hsa-miR-8073 affects YOD1

3 |

Hsa-miR-8073 decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

Hsa-miR-6839-5p affects YOD1

3 |

Hsa-miR-6839-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

23446348

Hsa-miR-6831-5p affects YOD1

3 |

Hsa-miR-6831-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-miR-6818-5p affects YOD1

3 |

Hsa-miR-6818-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-miR-5706 affects YOD1

3 |

Hsa-miR-5706 decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-miR-5582-3p affects YOD1

3 |

Hsa-miR-5582-3p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

27292025

➶

biopax:mirtarbase

No evidence text available

23592263

➶

biopax:mirtarbase

No evidence text available

23446348

Hsa-miR-548o-3p affects YOD1

3 |

Hsa-miR-548o-3p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

Hsa-miR-516b-5p affects YOD1

3 |

Hsa-miR-516b-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-miR-4782-5p affects YOD1

3 |

Hsa-miR-4782-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

21572407

Hsa-miR-4772-5p affects YOD1

3 |

Hsa-miR-4772-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-miR-4764-5p affects YOD1

3 |

Hsa-miR-4764-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-miR-4650-3p affects YOD1

3 |

Hsa-miR-4650-3p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

21572407

Hsa-miR-3927-3p affects YOD1

3 |

Hsa-miR-3927-3p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-miR-383-5p affects YOD1

3 |

Hsa-miR-383-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

21572407

Hsa-miR-378j affects YOD1

3 |

Hsa-miR-378j decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-miR-3653-3p affects YOD1

3 |

Hsa-miR-3653-3p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

Hsa-miR-3614-3p affects YOD1

3 |

Hsa-miR-3614-3p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-miR-3116 affects YOD1

3 |

Hsa-miR-3116 decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-miR-221-5p affects YOD1

3 |

Hsa-miR-221-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

23446348

Hsa-miR-2115-3p affects YOD1

3 |

Hsa-miR-2115-3p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-miR-21-5p affects YOD1

3 |

Hsa-miR-21-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

18591254

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

22227207

Hsa-miR-202-3p affects YOD1

3 |

Hsa-miR-202-3p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

21572407

Hsa-miR-196b-5p affects YOD1

3 |

Hsa-miR-196b-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-miR-196a-5p affects YOD1

3 |

Hsa-miR-196a-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-miR-1254 affects YOD1

3 |

Hsa-miR-1254 decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-miR-1237-3p affects YOD1

3 |

Hsa-miR-1237-3p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-let-7i-5p affects YOD1

3 |

Hsa-let-7i-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-let-7g-5p affects YOD1

3 |

Hsa-let-7g-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

21572407

Hsa-let-7f-5p affects YOD1

3 |

Hsa-let-7f-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

21572407

Hsa-let-7e-5p affects YOD1

3 |

Hsa-let-7e-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

21572407

Hsa-let-7d-5p affects YOD1

3 |

Hsa-let-7d-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-let-7c-5p affects YOD1

3 |

Hsa-let-7c-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

Hsa-let-7b-5p affects YOD1

3 |

Hsa-let-7b-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

23446348

Hsa-let-7a-5p affects YOD1

3 |

Hsa-let-7a-5p decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

21572407

Enzalutamide affects YOD1

| 3

Enzalutamide activates YOD1.

| 2

Enzalutamide activates YOD1. 2 / 2

| 2

➶

reach

"Furthermore, lentivirus-mediated transfection of two YOD1-specific short hairpin RNAs (shYOD1 #1 and shYOD1 #2) was used to inhibit YOD1 expression in 786-O cells."

40274778

YOD1 affects Ubiquitin

| 3

YOD1 inhibits Ubiquitin. 3 / 3

| 3

➶

reach

"Overexpression of YOD1 decreased endogenous WT ubiquitin of NLRP3, and Yod1 deficiency potentiated endogenous WT ubiquitin of NLRP3."

38789414

➶

reach

"In support of this model, over-expression of catalytically inactive YOD1 (referred to as C160S) has been shown to block the ubiquitin dependent degradation of model ERAD substrates [XREF_BIBR]."

24417208

➶

reach

"YOD1 antagonizes ubiquitin ligase TRAF6 and p62 dependent interleukin-1 signaling to NF-kappaB."

33343629

YOD1 affects UBXN6

3 |

YOD1 binds UBXN6. 3 / 3

3 |

➶

biogrid

No evidence text available

32296183

➶

biogrid

No evidence text available

27753622

➶

biogrid

No evidence text available

27753622

YOD1 affects TAFAZZIN

| 3

YOD1 activates TAFAZZIN. 3 / 3

| 3

➶

reach

"Deubiquitinase YOD1 potentiates YAP and TAZ activities through enhancing ITCH stability."

28416659

➶

reach

"Through stabilizing the E3 ligase of LATS, ubiquitin thioesterase OTU1 (YOD1) is also reported to modulate the Hippo–YAP/TAZ pathway31."

35024322

➶

reach

"Additionally, TAZ protein abundance was also indirectly modulated by other deubiquitinating enzymes such as USP9X [48] and YOD1 [49] via regulating Hippo signaling."

35931679

YOD1 affects ERK

| 3

YOD1 inhibits ERK. 3 / 3

| 3

➶

reach

"YOD1 stabilizes the E3 ligase TRIM33 and inhibits the ERK/beta-catenin signaling pathway."

37573347

➶

reach

40274778

➶

reach

"Using the western blot, we found that YOD1 inhibited the activation of ERK and β-catenin signaling pathways in HNSCC cell lines (Fig. 4J and Supplementary Fig. 3)."

37573347

YOD1 affects Colitis

| 3

YOD1 inhibits Colitis. 3 / 3

| 3

➶

reach

"The bone marrow transplantation experiment unambiguously confirmed that the aggravated colitis in Yod1 mice was predominantly caused by YOD1 deficiency in hematopoietic cells."

39333628

➶

reach

"Ablation of YOD1 abolishes the protective role of NOD2 signaling in DSS colitis."

39333628

➶

reach

"The bone marrow transplantation experiment reveals that YOD1 derived from hematopoietic cells inhibits DSS colitis."

39333628

YOD1 affects CS

| 3

CS binds YOD1. 3 / 3

| 3

➶

sparser

"This analysis demonstrated that upon heat shock, TorsinKO cells overexpressing YOD1-CS developed significantly fewer NE-associated K48-Ub foci ( xref G)."

35563018

➶

sparser

"While UBXD1, WT YOD1, and YOD1-CS are not enriched inside blebs ( xref ), MLF2 may contribute to bleb formation by interacting with these p97 adaptors elsewhere within the cell."

35563018

➶

sparser

"To test this possibility, we conducted an IP with an anti-FLAG antibody from TorsinKO cells expressing FLAG-tagged WT YOD1, YOD1-CS, UBXD1, or AIFM1 ( xref D)."

35563018

YOD1 affects CCDC51

3 |

CCDC51 binds YOD1. 3 / 3

3 |

➶

biogrid

No evidence text available

26186194

➶

biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

33961781

YOD1 affects AMFR

1 | 1 1

YOD1 binds AMFR. 3 / 3

1 | 1 1

➶

sparser

"By contrast, we failed to detect any YOD1-gp78 interaction."

24068323

➶

reach

"Accordingly, we first asked whether YOD1 binds to Hrd1 and gp78, as this DUB was shown to regulate the ERAD process."

24068323

➶

biogrid

No evidence text available

24068323

UBXN6 affects YOD1

3 |

YOD1 binds UBXN6. 3 / 3

3 |

➶

biogrid

No evidence text available

32296183

➶

biogrid

No evidence text available

27753622

➶

biogrid

No evidence text available

27753622

TRAF6 affects SQSTM1

| 3

TRAF6 binds YOD1 and SQSTM1. 3 / 3

| 3

➶

sparser

"By directly comparing the binding of YOD1 and p62 to TRAF6 in HEK293 cells we could confirm that both proteins are associating with the C-terminal MATH domain ( xref )."

28244869

➶

sparser

"Also, we quantified the amount of p62 and YOD1 bound to TRAF6 after double transfection (control, set to 1) and triple transfection (TRAF6, p62 and YOD1) in three independent experiments ( xref )."

28244869

➶

sparser

28244869

MIR661 affects YOD1

3 |

MIR661 decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

MIR583 affects YOD1

3 |

MIR583 decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

20371350

➶

biopax:mirtarbase

No evidence text available

23446348

MIR1323 affects YOD1

3 |

MIR1323 decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

MIR1294 affects YOD1

3 |

MIR1294 decreases the amount of YOD1. 3 / 3

3 |

➶

biopax:mirtarbase

No evidence text available

21572407

➶

biopax:mirtarbase

No evidence text available

23446348

➶

biopax:mirtarbase

No evidence text available

20371350

FAF2 affects DERL1

| 3

YOD1 binds DERL1 and FAF2. 3 / 3

| 3

➶

sparser

"In agreement with a catalytic role of YOD1 in protein dislocation, the catalytically inactive forms of YOD1 retrieved Derlin-1 and UBXD8 more efficiently than the WT counterpart."

19818707

➶

sparser

"Interaction between YOD1 and other components of ERAD such as Derlin-1 and Ubxd8 were also reported."

25013401

➶

sparser

"Immunoprecipitation experiments showed that YOD1 also interacts with the ERAD machinery components such as Derlin-1 and UBXD8."

22812527

E3_Ub_ligase affects YOD1

| 2 1

YOD1 binds E3_Ub_ligase. 3 / 3

| 2 1

➶

reach

"According to the results of liquid chromatography-mass spectrometry analysis (Fig. 4A), we found that the E3 ligase TRIM33 may bind to YOD1."

37573347

➶

reach

"The YOD1 deubiquitinase binds to the Hrd1 E3 ubiquitin ligase."

24068323

➶

sparser

"The YOD1 deubiquitinase binds to the Hrd1 E3 ubiquitin ligase."

24068323

DERL1 affects FAF2, and YOD1

| 3

YOD1 binds DERL1 and FAF2. 3 / 3

| 3

➶

sparser

"In agreement with a catalytic role of YOD1 in protein dislocation, the catalytically inactive forms of YOD1 retrieved Derlin-1 and UBXD8 more efficiently than the WT counterpart."

19818707

➶

sparser

"Interaction between YOD1 and other components of ERAD such as Derlin-1 and Ubxd8 were also reported."

25013401

➶

sparser

"Immunoprecipitation experiments showed that YOD1 also interacts with the ERAD machinery components such as Derlin-1 and UBXD8."

22812527

CS affects YOD1

| 3

CS binds YOD1. 3 / 3

| 3

➶

sparser

"This analysis demonstrated that upon heat shock, TorsinKO cells overexpressing YOD1-CS developed significantly fewer NE-associated K48-Ub foci ( xref G)."

35563018

➶

sparser

"While UBXD1, WT YOD1, and YOD1-CS are not enriched inside blebs ( xref ), MLF2 may contribute to bleb formation by interacting with these p97 adaptors elsewhere within the cell."

35563018

➶

sparser

"To test this possibility, we conducted an IP with an anti-FLAG antibody from TorsinKO cells expressing FLAG-tagged WT YOD1, YOD1-CS, UBXD1, or AIFM1 ( xref D)."

35563018

CCDC51 affects YOD1

3 |

CCDC51 binds YOD1. 3 / 3

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biogrid

No evidence text available

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biogrid

No evidence text available

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No evidence text available

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AMFR affects YOD1

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YOD1 binds AMFR. 3 / 3

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sparser

"By contrast, we failed to detect any YOD1-gp78 interaction."

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"Accordingly, we first asked whether YOD1 binds to Hrd1 and gp78, as this DUB was shown to regulate the ERAD process."

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biogrid

No evidence text available

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Α-synuclein affects YOD1

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YOD1 binds α-synuclein. 2 / 2

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"Next, we performed the GST pull-down assay to check the direct interaction between YOD1 and α-synuclein, and found that GST-YOD1 precipitates α-synuclein ( Fig. 1 B)."

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Valproic acid affects YOD1

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Valproic acid decreases the amount of YOD1. 2 / 2

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ctd

No evidence text available

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"In the present study, it was confirmed that miR-373 directly targets YOD1 by binding its 3 '-UTR."

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"For example, miR-373 increases proliferation of cervical cancer cells by directly targeting the highly conserved deubiquitinating enzyme of the ovarian tumor (otubain) family, YOD1 (25)."

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MiR-221/222 affects YOD1

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MiR-221/222 decreases the amount of YOD1.

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MiR-221/222 decreases the amount of YOD1. 1 / 1

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"We further demonstrated that the reduced expression of YOD1 was caused by overexpression of miR-221/222 and revealed that YOD1 overexpression augments the inhibitory effects of FLT3 tyrosine kinase inhibitors (TKIs) in AML cells."

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MiR-221/222 activates YOD1.

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YOD1 affects NOD2

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YOD1 activates NOD2. 2 / 2

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"Altogether, these findings show that YOD1 enhances NOD2 signaling in macrophages by stabilizing RIPK2."

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"We found that YOD1 directly interacts with α-synuclein and deubiquitinates K6-, K11-, K29-, K33-, and K63-linked polyubiquitin chains on α-synuclein."

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"These results demonstrate that YOD1 deubiquitinates K6-, K11-, K29-, K33-, and K63-linked polyubiquitin chains on α-synuclein.In the current study, we found that YOD1 deubiquitinates the K6-, K11-, K2[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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YOD1 affects IL1B

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YOD1 increases the amount of IL1B.

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YOD1 increases the amount of IL1B. 1 / 1

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"Results show that YOD1 knockdown inhibited the expression of TNF-α and IL-1β in 3 × Tg mice (Fig. 3I–K)."

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YOD1 decreases the amount of IL1B.

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YOD1 binds TRAF6, TRAF, and BLNK. 2 / 2

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TRAF6 affects IL1

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IL1 binds TRAF6 and YOD1. 2 / 2

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"Indeed, it seemed likely that in this overexpression scenario there was slightly augmented association of TRAF6 and YOD1 WT after short IL-1 stimulation."

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2,3,7,8-tetrachlorodibenzodioxine increases the amount of YOD1. 2 / 2

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ctd

No evidence text available

No evidence text available

33854195

IndraLab

Statements

YOD1 affects TRAF6

YOD1 binds TRAF6.

TRAF6 binds YOD1. 10 / 56

TRAF6 binds YOD1 and SQSTM1. 3 / 3

YOD1 binds TRAF6, TRAF, and BLNK. 2 / 2

IL1 binds TRAF6 and YOD1. 2 / 2

YOD1 inhibits TRAF6.

YOD1 inhibits TRAF6. 4 / 6

YOD1 ubiquitinates TRAF6.

YOD1 leads to the ubiquitination of TRAF6. 1 / 1

YOD1 affects cell population proliferation

YOD1 activates cell population proliferation.

YOD1 activates cell population proliferation. 10 / 18

YOD1 inhibits cell population proliferation.

YOD1 inhibits cell population proliferation. 10 / 17

YOD1 affects Circulin-C

YOD1 binds Circulin-C. 10 / 28

YOD1 affects CDK1

YOD1 binds CDK1.

YOD1 binds CDK1. 10 / 21

YOD1 binds CDK1 and RPS6. 1 / 1

YOD1 increases the amount of CDK1.

YOD1 increases the amount of CDK1. 3 / 3

YOD1 activates CDK1.

YOD1 activates CDK1. 2 / 2

YOD1 deubiquitinates CDK1.

YOD1 deubiquitinates CDK1. 1 / 1

Circulin-C affects YOD1

YOD1 binds Circulin-C. 10 / 28

YOD1 affects NLRP3

YOD1 binds NLRP3.

YOD1 binds NLRP3. 10 / 18

YOD1 decreases the amount of NLRP3.

YOD1 decreases the amount of NLRP3. 7 / 7

YOD1 deubiquitinates NLRP3.

YOD1 leads to the deubiquitination of NLRP3. 2 / 2

CDK1 affects YOD1

CDK1 binds YOD1.

YOD1 binds CDK1. 10 / 21

YOD1 binds CDK1 and RPS6. 1 / 1

CDK1 increases the amount of YOD1.

CDK1 increases the amount of YOD1. 1 / 1

YOD1 affects ZNF24

YOD1 binds ZNF24.

YOD1 binds ZNF24. 10 / 13

YOD1 deubiquitinates ZNF24.

YOD1 deubiquitinates ZNF24. 5 / 5

YOD1 inhibits ZNF24.

YOD1 inhibits ZNF24. 4 / 4

YOD1 affects USP25

YOD1 binds USP25.

YOD1 binds USP25. 10 / 15

YOD1 activates USP25.

YOD1 activates USP25. 5 / 5

NLRP3 affects YOD1

NLRP3 binds YOD1.

YOD1 binds NLRP3. 10 / 18

NLRP3 inhibits YOD1.

NLRP3 inhibits YOD1. 1 / 1

NLRP3 activates YOD1.

NLRP3 activates YOD1. 1 / 1

YOD1 affects SYVN1

YOD1 binds SYVN1.

YOD1 binds SYVN1. 10 / 14

YOD1 inhibits SYVN1.

YOD1 inhibits SYVN1. 2 / 2

YOD1 deubiquitinates SYVN1.

YOD1 deubiquitinates SYVN1. 2 / 2

YOD1 affects Flag

YOD1 binds Flag. 10 / 18

P97 affects YOD1

P97 binds YOD1. 10 / 13

YOD1 binds NPLOC4, Ufd1, and p97. 1 / 1

YOD1 binds Ubx and p97. 1 / 1

YOD1 affects VCP

YOD1 binds VCP.

YOD1 binds VCP. 10 / 14

YOD1 activates VCP.