IndraLab

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USP49 affects TP53
4 1 | 16 9
USP49 binds TP53.
4 | 6 9
USP49 binds TP53. 10 / 17
4 | 6 7
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"We next assessed whether USP49 binds to p53."
29748582
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"We therefore performed a co-immunoprecipitation experiment in which Flag-USP49 and HA-p53 plasmids were separately transfected or co-transfected into 293T cells and potential interactions between Flag-USP49 and HA-p53 were examined."
29748582
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"We also evaluated interaction between endogenous USP49 and p53 in HCT116 and U2OS cells using a p53 specific antibody."
29748582
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"We also evaluated interaction between endogenous USP49 and p53 in HCT116 and U2OS cells using a p53-specific antibody (Fig.  xref )."
29748582
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"We report that USP49 binds to and stabilizes p53 via deubiquitination."
29748582
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"USP49 interacts with p53."
29748582
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"In our study, we found that USP49 has a positive effect on the transcriptional activity and protein stability of p53 and that USP49 interacts with p53 and suppresses its ubiquitination."
29748582
sparser
"USP49 binds, deubiquitinates and stabilizes p53 following DNA damage, leading to p53 activation and apoptosis."
32201366
sparser
"We next assessed whether USP49 binds to p53."
29748582
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"In our study, we found that USP49 has a positive effect on the transcriptional activity and protein stability of p53 and that USP49 interacts with p53 and suppresses its ubiquitination."
29748582
TP53 binds GST, USP49, and Flag. 1 / 1
| 1
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"This interaction was further confirmed in a GST pull-down assay, with GST-p53 specifically interacting with transfected Flag-USP49, as shown in Fig.  xref ."
29748582
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"We therefore performed a co-immunoprecipitation experiment in which Flag-USP49 and HA-p53 plasmids were separately transfected or co-transfected into 293T cells and potential interactions between Flag-USP49 and HA-p53 were examined (Fig.  xref )."
29748582
USP49 deubiquitinates TP53.
1 | 3
USP49 leads to the deubiquitination of TP53. 4 / 4
1 | 3
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"USP49 suppresses p53 ubiquitination."
29748582
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"To assess this, we co-transfected HA-p53, Myc-ubiquitin, and Flag-USP49 into 293T cells, and found that USP49 suppressed ubiquitination of HA-p53."
29748582
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"Investigation of the mechanism revealed that USP49 interacts with the N terminus of p53 and suppresses several types of p53 ubiquitination."
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"In our study, we identified <span class="match term0">USP49</span> as a novel deubiquitinase (DUB) for <span class="match term1">p53</span> from a library consisting of 80 DUBs and found that <span class="match term0">USP49</span> has a positive effect on <span class="match term1">p53</span> transcriptional activity and protein stability"
29748582
USP49 activates TP53.
| 4
USP49 activates TP53. 4 / 4
| 4
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"Conversely, overexpression of USP49 suppressed MDM2 gene expression and then promoted TP53 target genes."
35072515
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"USP49 upregulates p53 protein stability."
29748582
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"However, knockdown of USP49 by shRNA reduced the level of p53 protein in HCT116 cells treated with or without Eto."
29748582
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"To explore the mechanism by which USP49 enhances p53 stability and taking into consideration that USP49 is a member of the USP family of deubiquitinating enzymes, we hypothesized that USP49 may have an effect on p53 ubiquitination."
29748582
USP49 increases the amount of TP53.
| 3
USP49 increases the amount of TP53. 2 / 2
| 2
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"Importantly, USP49 knockout stimulated MDM2 transcriptional level and then inhibited the mRNA levels of TP53 target genes."
35072515
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"Our findings suggest that the USP49/HDAC3 signaling axis up-regulates p53 expression by enhancing H3K18 lactylation, thereby inducing ferroptosis."
| PMC
Modified USP49 increases the amount of TP53. 1 / 1
| 1
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"Overexpression of USP49 downregulated the expression levels of Cyclin D1, and upregulated p53 expression."
31001918
USP49 activates cell population proliferation. 10 / 19
| 19
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"In sum, these results demonstrate that USP49 enhances malignant proliferation and facilitates CBP resistance of RB cells.3.3 USP49 accelerates xenograft tumor growth and induces CBP resistance in vivo."
39497328
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"Therefore, USP49 promotes RB tumor growth and CBP resistance in vivo.3.4 USP49 promotes cell proliferation and CBP resistance in RB cells via autophagy activation."
39497328
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"In this study, we verified that IGF2BP3‐dependent m A modification of USP49 promoted aggressive proliferation, inhibited apoptosis, and aggravated CBP resistance in RB by enhancing autophagy via SIRT1 deubiquitination.USP49 has been reported as an oncogene in several cancer types."
39497328
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"For instance, USP49 promotes the proliferation, metastasis, chemoresistance, and peritoneal metastasis in gastric cancer cells."
39497328
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"20 Furthermore, USP49 is upregulated in adenocarcinoma of the esophagogastric junction and promotes cancer cell proliferation."
39497328
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"Overexpression of USP49 promoted cell proliferation, inhibited cell apoptosis, and increased the IC50 value of CBP in RB cells."
39497328
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"On the contrary, USP49 knockdown suppressed cell proliferation, increased cell apoptosis, and reduced the IC50 value of CBP in CBP‐resistant RB cells."
39497328
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"In this study, ubiquitin-specific peptidase 49 (USP49) was identified as a novel deubiquitinase of YAP1, knockdown of USP49 inhibited the proliferation, metastasis, chemoresistance, and peritoneal metastasis of GC cells."
35318441
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"The present study showed that USP49 promotes cell proliferation, invasion, and antiapoptosis, suggesting a role as a tumor promoter in GBM."
34610959
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"USP49 silencing decreases cell proliferation, migration, and invasion and enhances GC cell sensitivity to chemotherapy; however, this effect can be reversed by YAP1 overexpression."
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"Additionally, overexpression of USP49 remarkably inhibits cell proliferation and regulates the PI3K/AKT signal pathway."
37849311
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"USP49 loss impairs cell proliferation and leads to aneuploidy."
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"Here, we report that the downregulation of Ubiquitin-specific peptidase 49 (USP49) promotes ferroptosis in OE33 and OE19 cells, thereby inhibiting cell proliferation in vitro and in vivo, whereas the overexpression of USP49 had the opposite effect."
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"Mechanistically, USP49 deubiquitinates and stabilizes FKBP51, which in turn enhances PHLPP 's capability to dephosphorylate AKT Furthermore, USP49 inhibited pancreatic cancer cell proliferation and enhanced cellular response to gemcitabine in a FKBP51-AKT-dependent manner."
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"Mechanistically, USP49 deubiquitinates and stabilizes FKBP51, which in turn enhances PHLPP's capability to dephosphorylate AKT Furthermore, USP49 inhibited pancreatic cancer cell proliferation and enhanced cellular response to gemcitabine in a FKBP51-AKT-dependent manner."
28363942
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"USP49 knockout contributes to increased HCT116 cell proliferation and migration."
35072515
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"In consistent with this notion, USP49 knockdown was recently reported to result in a decrease of cell proliferation and invasion as well as an increase of cell apoptosis in glioblastoma cells [ 25 ]."
35367823
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"As shown in Fig. 6D, overexpression of USP49 significantly inhibited cell proliferation and reversed the promotion effect of miR-5000-3p on cell proliferation ability.Due to USP49 was involved in the PI3K/AKT signaling pathway ."
34075026
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"A previous study suggested depletion of USP49 increased pancreatic cancer cell proliferation in vitro, tumorigenesis in vivo, and chemoresistance, proposing that USP49 regulates tumorigenesis and chemoresistance in pancreatic cancer in an AKT-dependent manner ."
34075026
USP49 affects PAX9
3 | 19 1
USP49 deubiquitinates PAX9.
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USP49 deubiquitinates PAX9. 7 / 7
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"We identify and demonstrate that USP49 interacts with and deubiquitinates PAX9 and MSX1, thereby extending their protein half-lives."
35273362
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"In sum, our results suggest that deubiquitination of PAX9 and MSX1 by USP49 stabilizes their protein levels to facilitate successful odontogenesis."
35273362
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"USP49 deubiquitinates PAX9 and MSX1 proteins."
35273362
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"USP49 significantly decreased the polyubiquitination of both endogenous PAX9 (Fig. 4B, lane 2) and MSX1 (Fig. 4E, lane 2), as well as exogenous PAX9 (Fig. 4C, lane 4) and MSX1 (Fig. 4F, lane 4)."
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"However, no deubiquitinating activity was observed for PAX9 and MSX1 proteins in the presence of USP49CA (Fig. 4B, lane 3; 4E, lane 3; Fig. 4C, lane 5; Fig. 4F, lane 5), upon knockdown of USP49 (Fig. 4B, lane 4; 4E, lane 4; Fig. 4C, lane 6; Fig. 4F, lane 6), USP44, a paralog of USP49 [38] (Supplementary Fig. S9A, B, lane 6), indicating that USP49 deubiquitinates PAX9 and MSX1."
35273362
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"Importantly, the loss of USP49 promoted the polyubiquitination of PAX9 and MSX1, thereby triggering rapid protein degradation (Fig. 4)."
35273362
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"Here, we demonstrated that USP49 with the UCH domain at the C-terminus is necessary for the interaction and deubiquitination of PAX9 and MSX1."
35273362
USP49 binds PAX9.
3 | 3 1
PAX9 binds USP49. 7 / 7
3 | 3 1
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"Likewise, N-terminus USP49 showed similar ALP staining and intracellular ALP activity as mock-control (Fig. 5L, M), indicating that the interaction between USP49 and PAX9 or MSX1 is essential for odontogenesis."
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"The latter suggests that the interaction between USP49 and PAX9 or MSX1 is essential for normal odontogenesis (Fig. 5)."
35273362
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"Additionally, we demonstrated USP49-PAX9 and USP49-MSX1 interactions using the Duolink PLA assay (Fig.  xref )."
35273362
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No evidence text available
35273362
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No evidence text available
35273362
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"We identify and demonstrate that USP49 interacts with and deubiquitinates PAX9 and MSX1, thereby extending their protein half-lives."
35273362
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No evidence text available
35273362
USP49 increases the amount of PAX9.
| 4
USP49 increases the amount of PAX9. 4 / 4
| 4
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"USP49 increases PAX9 and MSX1 protein levels."
35273362
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"Transient transfection of both sgRNA and shRNA targeting USP49 significantly decreased the expression of endogenous PAX9 and MSX1 compared to the mock control (Fig. 2C, lanes 2 and 5)."
35273362
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"Furthermore, overexpression of USP49 in hDPSCs dose-dependently increased the expression of endogenous PAX9 and MSX1 proteins (Fig. 2D)."
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"On the other hand, the loss of USP49 reduces the levels of PAX9 and MSX1 proteins, which causes transient retardation of odontogenic differentiation in human dental pulp stem cells and delays the differentiation of human pluripotent stem cells into the neural crest cell lineage."
35273362
USP49 activates PAX9.
| 3
USP49 activates PAX9. 3 / 3
| 3
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"Thus, PAX9 and MSX1 ubiquitination was elevated in USP49 KO-hDPSCs, indicating that the loss of USP49 promotes PAX9 and MSX1 protein degradation."
35273362
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"USP49 promotes the odontogenic differentiation of hDPSCs by stabilizing PAX9 and MSX1."
35273362
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"Similarly, in HEK293 cells, we observed a USP49-mediated increase in the expression of Myc-PAX9 and Flag-MSX1 (Supplementary Fig. S3C, D), whereas USP49CA had no such effect (Supplementary Fig. S3E, F), suggesting that USP49 increases PAX9 and MSX1 protein levels.Additionally, the reduced expression of endogenous PAX9 and MSX1 proteins caused by sgRNA or shRNA targeting USP49 was rescued when hDPSCs were reconstituted with USP49 (Fig. 2F, lanes 4 and 7)."
35273362
USP49 decreases the amount of PAX9.
| 2
USP49 decreases the amount of PAX9. 2 / 2
| 2
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"On the other hand, the loss of USP49 reduces the levels of PAX9 and MSX1 proteins, which causes transient retardation of odontogenic differentiation in human dental pulp stem cells and delays the differentiation of human pluripotent stem cells into the neural crest cell lineage."
35273362
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"Moreover, immunostaining showed a complete reduction in USP49 expression in the USP49 KO-clone, which reduced PAX9 and MSX1 expression (Fig. 6C)."
35273362
USP49 affects MSX1
3 | 19 1
USP49 binds MSX1.
3 | 2 1
MSX1 binds USP49. 6 / 6
3 | 2 1
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"The latter suggests that the interaction between USP49 and PAX9 or MSX1 is essential for normal odontogenesis (Fig. 5)."
35273362
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No evidence text available
35273362
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"Additionally, we demonstrated USP49-PAX9 and USP49-MSX1 interactions using the Duolink PLA assay (Fig.  xref )."
35273362
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No evidence text available
35273362
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"Likewise, N-terminus USP49 showed similar ALP staining and intracellular ALP activity as mock-control (Fig. 5L, M), indicating that the interaction between USP49 and PAX9 or MSX1 is essential for odontogenesis."
35273362
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No evidence text available
35273362
USP49 deubiquitinates MSX1.
| 5
USP49 deubiquitinates MSX1. 5 / 5
| 5
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"In sum, our results suggest that deubiquitination of PAX9 and MSX1 by USP49 stabilizes their protein levels to facilitate successful odontogenesis."
35273362
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"USP49 deubiquitinates PAX9 and MSX1 proteins."
35273362
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"However, no deubiquitinating activity was observed for PAX9 and MSX1 proteins in the presence of USP49CA (Fig. 4B, lane 3; 4E, lane 3; Fig. 4C, lane 5; Fig. 4F, lane 5), upon knockdown of USP49 (Fig. 4B, lane 4; 4E, lane 4; Fig. 4C, lane 6; Fig. 4F, lane 6), USP44, a paralog of USP49 [38] (Supplementary Fig. S9A, B, lane 6), indicating that USP49 deubiquitinates PAX9 and MSX1."
35273362
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"Importantly, the loss of USP49 promoted the polyubiquitination of PAX9 and MSX1, thereby triggering rapid protein degradation (Fig. 4)."
35273362
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"Here, we demonstrated that USP49 with the UCH domain at the C-terminus is necessary for the interaction and deubiquitination of PAX9 and MSX1."
35273362
USP49 increases the amount of MSX1.
| 4
USP49 increases the amount of MSX1. 4 / 4
| 4
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"USP49 increases PAX9 and MSX1 protein levels."
35273362
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"Transient transfection of both sgRNA and shRNA targeting USP49 significantly decreased the expression of endogenous PAX9 and MSX1 compared to the mock control (Fig. 2C, lanes 2 and 5)."
35273362
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"On the other hand, the loss of USP49 reduces the levels of PAX9 and MSX1 proteins, which causes transient retardation of odontogenic differentiation in human dental pulp stem cells and delays the differentiation of human pluripotent stem cells into the neural crest cell lineage."
35273362
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"Furthermore, overexpression of USP49 in hDPSCs dose-dependently increased the expression of endogenous PAX9 and MSX1 proteins (Fig. 2D)."
35273362
USP49 decreases the amount of MSX1.
| 4
USP49 decreases the amount of MSX1. 4 / 4
| 4
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"On the other hand, the loss of USP49 reduces the levels of PAX9 and MSX1 proteins, which causes transient retardation of odontogenic differentiation in human dental pulp stem cells and delays the differentiation of human pluripotent stem cells into the neural crest cell lineage."
35273362
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"Moreover, immunostaining showed a complete reduction in USP49 expression in the USP49 KO-clone, which reduced PAX9 and MSX1 expression (Fig. 6C)."
35273362
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"Disruption of USP13 and USP49 genes reduced PAX9 protein, and USP11 and USP49 disruption reduced MSX1 protein levels (Fig. 1A)."
35273362
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"USP49 expression was significantly reduced in USP49 KO-clone#28, resulting in reduced PAX9 and MSX1 expression in USP49 KO-clone#28 compared with the mock control (Supplementary Fig. S12G)."
35273362
USP49 activates MSX1.
| 3
USP49 activates MSX1. 3 / 3
| 3
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"Thus, PAX9 and MSX1 ubiquitination was elevated in USP49 KO-hDPSCs, indicating that the loss of USP49 promotes PAX9 and MSX1 protein degradation."
35273362
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"USP49 promotes the odontogenic differentiation of hDPSCs by stabilizing PAX9 and MSX1."
35273362
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"Similarly, in HEK293 cells, we observed a USP49-mediated increase in the expression of Myc-PAX9 and Flag-MSX1 (Supplementary Fig. S3C, D), whereas USP49CA had no such effect (Supplementary Fig. S3E, F), suggesting that USP49 increases PAX9 and MSX1 protein levels.Additionally, the reduced expression of endogenous PAX9 and MSX1 proteins caused by sgRNA or shRNA targeting USP49 was rescued when hDPSCs were reconstituted with USP49 (Fig. 2F, lanes 4 and 7)."
35273362
USP49 inhibits MSX1.
| 1
USP49 inhibits MSX1. 1 / 1
| 1
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"Similarly, an immunoprecipitation analysis of HEK293 cells co-transfected with Flag-MSX1 and HA-ubiquitin showed polyubiquitination of the MSX1 protein (Supplementary Fig. S8F, lane 4), and the TUBEs assay revealed that the loss of USP49 produced a higher ubiquitination status for MSX1 (Fig. 4D, lane 3) than mock-hDPSCs (Fig. 4D, lane 2)."
35273362
TP53 affects USP49
4 | 7 9
TP53 binds USP49.
4 | 6 9
USP49 binds TP53. 10 / 17
4 | 6 7
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"We next assessed whether USP49 binds to p53."
29748582
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"We therefore performed a co-immunoprecipitation experiment in which Flag-USP49 and HA-p53 plasmids were separately transfected or co-transfected into 293T cells and potential interactions between Flag-USP49 and HA-p53 were examined."
29748582
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"We also evaluated interaction between endogenous USP49 and p53 in HCT116 and U2OS cells using a p53 specific antibody."
29748582
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"We also evaluated interaction between endogenous USP49 and p53 in HCT116 and U2OS cells using a p53-specific antibody (Fig.  xref )."
29748582
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"We report that USP49 binds to and stabilizes p53 via deubiquitination."
29748582
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"USP49 interacts with p53."
29748582
sparser
"In our study, we found that USP49 has a positive effect on the transcriptional activity and protein stability of p53 and that USP49 interacts with p53 and suppresses its ubiquitination."
29748582
sparser
"USP49 binds, deubiquitinates and stabilizes p53 following DNA damage, leading to p53 activation and apoptosis."
32201366
sparser
"We next assessed whether USP49 binds to p53."
29748582
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"In our study, we found that USP49 has a positive effect on the transcriptional activity and protein stability of p53 and that USP49 interacts with p53 and suppresses its ubiquitination."
29748582
TP53 binds GST, USP49, and Flag. 1 / 1
| 1
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"This interaction was further confirmed in a GST pull-down assay, with GST-p53 specifically interacting with transfected Flag-USP49, as shown in Fig.  xref ."
29748582
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"We therefore performed a co-immunoprecipitation experiment in which Flag-USP49 and HA-p53 plasmids were separately transfected or co-transfected into 293T cells and potential interactions between Flag-USP49 and HA-p53 were examined (Fig.  xref )."
29748582
TP53 increases the amount of USP49.
| 1
TP53 increases the amount of USP49. 1 / 1
| 1
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"In response to DNA damage, p53 promotes the expression of USP49 (Tu et al., 2018)."
31282934
USP49 affects HDAC3
| 11 8
USP49 binds HDAC3.
| 7 8
USP49 binds HDAC3. 10 / 15
| 7 8
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"Immunoprecipitation-mass spectrometry, co-immunoprecipitation, immunofluorescence co-localization, and molecular docking were used to analyze USP49 and HDAC3 interactions."
| PMC
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"The interaction between USP49 and HDAC3 was validated through mass spectrometry and co-immunoprecipitation experiments."
| PMC
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"Then we used co-IP experiments to verify that there is indeed an interaction between HDAC3 and USP49 in the HEC-1B cell (Fig. 5B)."
| PMC
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"Furthermore, the three-dimensional complex model for protein docking prediction demonstrated a robust interaction between HDAC3 and USP49 (Fig. 5D)."
| PMC
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"Our study explored the HDAC3 and USP49 interaction through mass spectrometry and co-immunoprecipitation."
| PMC
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"Immunoprecipitation-mass spectrometry, co-immunoprecipitation, immunofluorescence co-localization, and molecular docking were used to analyze USP49 and HDAC3 interactions."
| PMC
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"The interaction between USP49 and HDAC3 was validated through mass spectrometry and co-immunoprecipitation experiments."
| PMC
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"The deubiquitinase USP49 interacts with HDAC3 and enhances its stability."
| PMC
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"Then we used co-IP experiments to verify that there is indeed an interaction between HDAC3 and USP49 in the HEC-1B cell (Fig.  xref B)."
| PMC
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"Furthermore, the three-dimensional complex model for protein docking prediction demonstrated a robust interaction between HDAC3 and USP49 (Fig.  xref D)."
| PMC
USP49 ubiquitinates HDAC3.
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USP49 leads to the ubiquitination of HDAC3. 2 / 2
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"The regulation of HDAC3 by CAP is contingent upon USP49, wherein the down-regulation of USP49 augments the ubiquitination of HDAC3, consequently diminishing its protein stability."
40234906
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"The regulation of HDAC3 by CAP is contingent upon USP49, wherein the down-regulation of USP49 augments the ubiquitination of HDAC3, consequently diminishing its protein stability."
| PMC
USP49 increases the amount of HDAC3.
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USP49 increases the amount of HDAC3. 1 / 1
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"USP49 promotes endometrial cancer progression by regulating HDAC3 expression."
| PMC
USP49 decreases the amount of HDAC3.
| 1
USP49 decreases the amount of HDAC3. 1 / 1
| 1
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"Our results indicated that overexpression of USP49 reduced the ubiquitination level of HDAC3 (Fig. 5O)."
| PMC
USP49 affects STING1
4 1 | 3 9
USP49 binds STING1.
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USP49 binds STING1. 10 / 13
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"USP49, a ubiquitin-specific protease, interacts with and deubiquitinates STING after HSV-1 infection."
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"These data together suggest that USP49 interacts with MITA and inhibits MITA-mediated signaling."
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"In an unbiased screening of MITA-interacting DUBs, we have found that USP49 interacts with MITA [ xref ]."
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"In this study, we demonstrated that USP49 interacted with MITA and deconjugated K63-linked polyubiquitin chains from MITA after HSV-1 infection, thereby downregulating MITA activation and turning down immune responses ( xref )."
30943264
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"USP49 interacts with MITA and inhibits MITA-mediated signaling."
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No evidence text available
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"In this context, we observed that USP49-MITA association was induced by HSV-1 infection and the basal expression of type I IFNs or proinflammatory cytokines was comparable between the wild-type and Usp49 -/- cells."
30943264
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"To test our hypothesis, we conducted an unbiased screening of MITA-interacting DUBs by cotransfection and immunoprecipitation assays and found that USP49 interacted with MITA [ xref ]."
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No evidence text available
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"Whether and how the USP49-MITA axis regulates inflammatory diseases such as colitis and tumorigenesis such as colon cancer is of great interest and requires future investigations."
30943264
USP49 deubiquitinates STING1.
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USP49 deubiquitinates STING1. 3 / 3
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"USP49, a ubiquitin-specific protease, interacts with and deubiquitinates STING after HSV-1 infection."
35033428
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"The USP family of DUBs is very important, with USP13, USP18, USP20, USP21, USP44, and USP49 shown to mediate the deubiquitination of STING (Chen et al., 2021)."
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"Mechanistically, <span class="match term0">USP49</span> removes K63-linked ubiquitin chains from <span class="match term1">MITA</span> after HSV-1 infection which inhibits the aggregation of <span class="match term1">MITA</span> and the subsequent recruitment of TBK1 to the signaling complex"
30943264
USP49 ubiquitinates STING1.
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USP49 ubiquitinates STING1. 1 / 1
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"Furthermore, we found that reconstitution of USP49 but not USP49 (C262A) into Usp49 -/- MLFs inhibited HSV-1-induced K63-linked ubiquitination of MITA ( xref )."
30943264
USP49 affects H2AX
1 | 12 2
USP49 deubiquitinates H2AX.
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USP49 deubiquitinates H2AX. 7 / 7
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"These results suggested that over-expression of USP49 suppressed phleomycin-induced γH2AX ubiquitylation in a DUB activity dependent manner."
35598681
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"These results suggest that USP49 negatively regulates ubiquitylation of γH2AX not in a ZnF-UBP domain but a DUB activity dependent manner.To further understand the involvement of USP49 in DDR, we exam[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"In addition, we found that USP49 negatively regulates DSB-induced γH2AX ubiquitylation ( Fig. 1 B)."
35598681
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"1) Over-expression of USP49 suppressed DSB-induced ubiquitylation of γH2AX in its catalytic activity dependent manner."
35598681
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"This could suggest that USP49 suppresses 53BP1 binding to chromatin by deubiquitylating γH2AX when cells were not challenged."
35598681
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"Phleomycin treatment induced ubiquitylation of γH2AX that was suppressed by the transient over-expression of GFP-USP49 (WT) in comparison to GFP expression ( Fig. 1 B)."
35598681
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"Contrary, γH2AX ubiquitylation was not suppressed by the transient over-expression of a catalytically inactive mutant GFP-USP49 [GFP-USP49 (CA)] in which Cys262 was replaced with Ala ( Fig. 1 B) ( Zha[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35598681
USP49 ubiquitinates H2AX.
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USP49 ubiquitinates H2AX. 4 / 4
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"1) Over-expression of USP49 suppressed DSB-induced ubiquitylation of γH2AX in its catalytic activity dependent manner."
35598681
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"This could suggest that USP49 suppresses 53BP1 binding to chromatin by deubiquitylating γH2AX when cells were not challenged."
35598681
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"In addition, we found that USP49 negatively regulates DSB-induced γH2AX ubiquitylation ( Fig. 1 B)."
35598681
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"Importantly, similar results were obtained with IR-irradiated cells ( Supplementary Fig. S2 ), suggesting that excess amount of USP49 antagonized DSB-induced 53BP1 foci formation by suppressing γH2AX [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35598681
USP49 binds H2AX.
1 | 1 2
USP49 binds H2AX. 3 / 3
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"Although recruitment of GFP-USP49 (WT) to DSB sites was not detectable, this may be because of transient interaction between USP49 and a potential substrate such as ubiquitylated-γH2AX."
35598681
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"The colocalization of USP49 (CA) and γH2AX prompted us to investigate the protein–protein interaction between USP49 (CA) and γH2AX ( Fig. 2 C)."
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No evidence text available
35598681
USP49 binds H2AX and 2 C. 1 / 1
| 1
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"The colocalization of USP49 (CA) and γH2AX prompted us to investigate the protein–protein interaction between USP49 (CA) and γH2AX ( Fig. 2 C)."
35598681
USP49 is modified
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USP49 is ubiquitinated.
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USP49 is ubiquitinated. 9 / 9
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"Furthermore, our results also demonstrated that Fbxo45 promoted USP49 ubiquitination (Fig. xref and Supplementary Fig. xref )."
35279684
sparser
"Furthermore, deletion of SPRY abrogated the ubiquitination of USP49 compared with the F-box deletion and WT of Fbxo45 in Panc-1 cells and 293 T cells (Fig. xref , Supplementary Fig. xref )."
35279684
sparser
"Moreover, ubiquitination of USP49 was dramatically inhibited in USP49 del but not in WT (Fig. xref and Supplementary Fig. xref )."
35279684
sparser
"Furthermore, we found that reconstitution of USP49 but not USP49 (C262A) into Usp49 -/- MLFs inhibited HSV-1-induced K63-linked ubiquitination of MITA ( xref )."
30943264
sparser
"More importantly, NEK6 strengthened the Fbxo45-mediated ubiquitination of USP49 WT, while NEK6 had no influence on the ubiquitination of USP49 del (Fig. xref and Supplementary Fig. xref )."
35279684
sparser
"These results indicated that NEK6 has a potential role in the promotion of Fbxo45-mediated ubiquitination and degradation of USP49."
35279684
sparser
"Furthermore, Fbxo45 exerted its oncogenic function partly via promotion of USP49 ubiquitination and degradation."
35279684
sparser
"We observed that Fbxo45 could bind to specific degrons of USP49 through its SPRY domain and enhance USP49 ubiquitination, subsequently leading to USP49 protein destruction in a posttranslational manner."
35279684
sparser
"Wu et al. reported that FBXO45 regulated the ubiquitination and degradation of USP49 and accelerated the tumor progression in pancreatic cancer [ xref ]."
38773471
USP49 is methylated.
| 4
USP49 is methylated. 4 / 4
| 4
sparser
"Besides, Me‐RIP analysis demonstrated that IGF2BP3 overexpression significantly enhanced the m 6 A methylation level of USP49, whereas IGF2BP3 knockdown reduced the m 6 A methylation of USP49 (Figure  xref )."
39497328
sparser
"Blood and saliva displayed over 90% methylation in USP49, DACT1 and PFN3."
25732744
sparser
"From this data, the authors suggested that low methylation of HOXA4, and high methylation of PRMT2, USP49 and DACT1 may be used to confirm the presence of vaginal fluid and menstrual blood."
25732744
sparser
"Levels of methylation in DACT1 and USP49 were significantly higher than PRMT2 and PFN3 for vaginal fluid and menstrual blood."
25732744
USP49 is phosphorylated.
| 2
USP49 is phosphorylated. 2 / 2
| 2
sparser
"To identify specific kinase(s) required for USP49 degradation, we used the Scansite program to predict the proper kinase(s) responsible for phosphorylation of USP49."
35279684
sparser
"Some NEKs, including NEK1, NEK2, NEK4, and NEK5, were observed to be involved in USP49 Ser/Thr residue phosphorylation by the Scansite program."
35279684
HDAC3 affects USP49
| 7 8
USP49 binds HDAC3. 10 / 15
| 7 8
reach
"Immunoprecipitation-mass spectrometry, co-immunoprecipitation, immunofluorescence co-localization, and molecular docking were used to analyze USP49 and HDAC3 interactions."
| PMC
reach
"The interaction between USP49 and HDAC3 was validated through mass spectrometry and co-immunoprecipitation experiments."
| PMC
reach
"Then we used co-IP experiments to verify that there is indeed an interaction between HDAC3 and USP49 in the HEC-1B cell (Fig. 5B)."
| PMC
reach
"Furthermore, the three-dimensional complex model for protein docking prediction demonstrated a robust interaction between HDAC3 and USP49 (Fig. 5D)."
| PMC
reach
"Our study explored the HDAC3 and USP49 interaction through mass spectrometry and co-immunoprecipitation."
| PMC
sparser
"Immunoprecipitation-mass spectrometry, co-immunoprecipitation, immunofluorescence co-localization, and molecular docking were used to analyze USP49 and HDAC3 interactions."
| PMC
sparser
"The interaction between USP49 and HDAC3 was validated through mass spectrometry and co-immunoprecipitation experiments."
| PMC
sparser
"The deubiquitinase USP49 interacts with HDAC3 and enhances its stability."
| PMC
sparser
"Then we used co-IP experiments to verify that there is indeed an interaction between HDAC3 and USP49 in the HEC-1B cell (Fig.  xref B)."
| PMC
sparser
"Furthermore, the three-dimensional complex model for protein docking prediction demonstrated a robust interaction between HDAC3 and USP49 (Fig.  xref D)."
| PMC
STING1 affects USP49
4 | 1 8
USP49 binds STING1. 10 / 13
4 | 1 8
reach
"USP49, a ubiquitin-specific protease, interacts with and deubiquitinates STING after HSV-1 infection."
35033428
sparser
"These data together suggest that USP49 interacts with MITA and inhibits MITA-mediated signaling."
30943264
sparser
"In an unbiased screening of MITA-interacting DUBs, we have found that USP49 interacts with MITA [ xref ]."
30943264
sparser
"In this study, we demonstrated that USP49 interacted with MITA and deconjugated K63-linked polyubiquitin chains from MITA after HSV-1 infection, thereby downregulating MITA activation and turning down immune responses ( xref )."
30943264
sparser
"USP49 interacts with MITA and inhibits MITA-mediated signaling."
30943264
biogrid
No evidence text available
27801882
sparser
"In this context, we observed that USP49-MITA association was induced by HSV-1 infection and the basal expression of type I IFNs or proinflammatory cytokines was comparable between the wild-type and Usp49 -/- cells."
30943264
sparser
"To test our hypothesis, we conducted an unbiased screening of MITA-interacting DUBs by cotransfection and immunoprecipitation assays and found that USP49 interacted with MITA [ xref ]."
30943264
biogrid
No evidence text available
30943264
sparser
"Whether and how the USP49-MITA axis regulates inflammatory diseases such as colitis and tumorigenesis such as colon cancer is of great interest and requires future investigations."
30943264
USP49 affects BAG2
3 | 8 1
USP49 binds BAG2.
3 | 1 1
USP49 binds BAG2. 5 / 5
3 | 1 1
reach
"Moreover, co-immunoprecipitation using HCT116 cell lysates validated this specific interaction between endogenous BAG2 and USP49 in CRC cells ( Fig. 4 E)."
35367823
sparser
"Co-immunoprecipatation (co-IP) assay identified the interaction between USP49-BAG2 in 293T cells expressing Flag-USP49 and HA-BAG2 ( Fig. 4 D)."
35367823
biogrid
No evidence text available
35367823
biogrid
No evidence text available
35367823
biogrid
No evidence text available
35367823
USP49 deubiquitinates BAG2.
| 3
USP49 deubiquitinates BAG2. 3 / 3
| 3
reach
"20 For instance, USP49 deubiquitinates and stabilizes Bcl‐2‐Associated Athanogene 2 (BAG2) which is a well‐known oncogenic protein that antagonizes apoptosis and enables adaptive response in colorectal cancer."
39497328
reach
"USP49 promotes the proliferation and chemoresistance of CRC cells by deubiquitinating and stabilizing Bcl-2-associated athanogene 2 (BAG2)."
38292842
reach
"In addition, BAG2 is deubiquitinated and stabilized by USP49, and knockdown of USP49 markedly inhibits colorectal cancer proliferation, colony formation, and chemoresistance resistance in vitro 19."
36593950
USP49 activates BAG2.
| 2
USP49 activates BAG2. 2 / 2
| 2
reach
"To the best of our knowledge, this is the first study that identified deubiquitinase regulating BAG2.In summary, we revealed an oncogenic role of USP49 in CRC cells and identified a novel mechanism by[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35367823
reach
"As shown in Fig. 4 G, overexpression of USP49 increased the BAG2 abundance in a dose-dependent manner."
35367823
USP49 increases the amount of BAG2.
| 1
USP49 increases the amount of BAG2. 1 / 1
| 1
reach
"We then knockdowned the endogenous USP49 by specific shRNAs, and found that inhibition of USP49 decreased the endogenous BAG2 level, indicating that USP49 is a key regulator for BAG2 stability in vivo[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35367823
USP49 decreases the amount of BAG2.
| 1
USP49 decreases the amount of BAG2. 1 / 1
| 1
reach
"As expected, USP49 significantly suppressed the polyubiquitination level of BAG2 upon overexpression in 293T cells ( Fig. 4 F)."
35367823
USP49 affects SIRT1
| 9 2
USP49 increases the amount of SIRT1.
| 4
USP49 increases the amount of SIRT1. 4 / 4
| 4
reach
"We further observed that IGF2BP3 overexpression enhanced SIRT1 protein level which was reversed by USP49 knockdown (Figure 7L)."
39497328
reach
"As USP49 serves as a deubiquitinating enzyme, 25 we speculated whether USP49 positively regulated SIRT1 protein level by protecting SIRT1 from proteasome‐dependent degradation."
39497328
reach
"In addition, IGF2BP3 overexpression enhanced SIRT1 protein level which was reversed by USP49 knockdown."
39497328
reach
"Impressively, USP49 silencing decreased SIRT1 protein level in Y‐79/CBP cells; moreover, the decrease in SIRT1 protein level could be reversed by the addition of proteasome inhibitor MG‐132 (Figure 5E), indicating SIRT1 may be degraded in a ubiquitin‐proteasome manner."
39497328
USP49 binds SIRT1.
| 2 2
USP49 binds SIRT1. 4 / 4
| 2 2
sparser
"We first confirmed the interaction between USP49 and SIRT1 by Co‐IP."
39497328
sparser
"Taken together, these results suggested that USP49 interacted with SIRT1 and inhibited SIRT1 ubiquitination and degradation in RB."
39497328
reach
"As shown in Figure 5C,D, USP49 co‐immunoprecipitated with SIRT1, suggesting an interaction between USP49 and SIRT1 in Y‐79/CBP cells."
39497328
reach
"We first confirmed the interaction between USP49 and SIRT1 by Co‐IP."
39497328
USP49 activates SIRT1.
| 2
USP49 activates SIRT1. 2 / 2
| 2
reach
"Furthermore, it was also revealed that IGF2BP3‐dependent m6A modification of USP49 enhances autophagy by stabilizing SIRT1 via its deubiquitination, helping RB cells survive under CBP treatment."
39497328
reach
"Our findings revealed that IGF2BP3‐dependent m A modification of USP49 mRNA upregulates USP49 expression to enhance SIRT1 stability.These findings highlight the critical role of USP49 in cellular processes and its potential as a therapeutic target in RB."
39497328
USP49 decreases the amount of SIRT1.
| 1
USP49 decreases the amount of SIRT1. 1 / 1
| 1
reach
"Furthermore, USP49 knockdown remarkably increased the ubiquitination level of SIRT1 in Y‐79/CBP cells (Figure 5G)."
39497328
USP49 affects APOBEC3G
2 1 | 8
USP49 activates APOBEC3G.
| 6
USP49 activates APOBEC3G. 6 / 6
| 6
eidos
"USP49 enhanced A3G levels in both cell lysates and viral particles ( Figure 3D ) ."
31397674
eidos
"USP49 enhances the expression of A3G even in absence of Vif ."
31397674
eidos
"USP49 directly interacts with A3G and deubiquitinates the K48-linked ubiquitination of A3G mediated by vif Considering that USP49 could enhance the expression of A3G directly , we next assessed whether USP49 binds A3G ."
31397674
eidos
"This result verifies that the inhibition of USP49 can promote the degradation of A3G and subsequently reduce the hypermutation in virus ."
31397674
eidos
"This result verifies that the inhibition of USP49 can promote the degradation of A3G and subsequently reduce the hypermutation in virus.These data indicated that the anti-A3G effect of Vif can be enforced by depletion of the A3G protein stabilizer USP49 , alternatively supporting the fact that USP49 enhances the anti-HIV-1 effect of A3G ."
31397674
eidos
"USP49 enhances the expression of A3G even in the absence of HIV-1 vif Unexpectedly , in the absence of Vif , USP49 knockdown with siRNA also significantly decreased A3G stability , which could be rescued by MG132 ( Figure 3A , B ) , suggesting that A3G could be degraded through a Vif-independent pathway ."
31397674
USP49 inhibits APOBEC3G.
| 2
USP49 inhibits APOBEC3G. 2 / 2
| 2
eidos
"It is stated that USP49 significantly suppressed downregulation of A3G at moderate levels of Vif , but the effect was limited at high levels of Vif ."
31397674
eidos
"USP49 interacted with and removes ubiquitins from A3G , and thereby prevents the degradation of A3G ."
31397674
USP49 binds APOBEC3G.
2 |
USP49 binds APOBEC3G. 2 / 2
2 |
biogrid
No evidence text available
31397674
biogrid
No evidence text available
31397674
USP49 deubiquitinates APOBEC3G.
1 |
USP49 deubiquitinates APOBEC3G. 1 / 1
1 |
ubibrowser
"Here, we found that <span class="match term0">USP49</span> directly interacts with <span class="match term1">A3G</span> and efficiently removes ubiquitin, consequently increasing <span class="match term1">A3G</span> protein expression and significantly enhancing its anti-HIV-1 activity"
31397674
USP49 affects apoptotic process
| 10
USP49 inhibits apoptotic process.
| 6
USP49 inhibits apoptotic process. 6 / 6
| 6
reach
"Furthermore, USP49 overexpression in Y‐79 cells increased cell proliferation and inhibited cell apoptosis, whereas the knockdown of USP49 in Y‐79/CBP cells exerted an opposite effect (Figure 2F–H)."
39497328
reach
"Overexpression of USP49 promoted cell proliferation, inhibited cell apoptosis, and increased the IC50 value of CBP in RB cells."
39497328
reach
"Besides, USP49 inhibits I/R‐induced cell viability suppression and apoptosis in AC16 cells via the DUSP1‐JNK1/2 pathways (Zhang et al., 2019)."
34694031
reach
"USP49 facilitated the deubiquitination of Axin and suppressed IL-1β-stimulated apoptosis of chondrocytes by inhibition of Wnt/β-catenin signaling [7]."
37814350
reach
"This demonstrated that USP49 knockdown significantly increased cell apoptosis, while GRβ introduction overcame this effect."
34610959
reach
"USP49 inhibits ischemia-reperfusion-induced cell viability suppression and apoptosis in human AC16 cardiomyocytes through DUSP1-JNK1/2 signaling."
30246457
USP49 activates apoptotic process.
| 4
USP49 activates apoptotic process. 4 / 4
| 4
reach
"USP49 knockdown Inhibits JNK1/2 activation and cell apoptosis [192]."
40102846
reach
"On the contrary, USP49 knockdown suppressed cell proliferation, increased cell apoptosis, and reduced the IC50 value of CBP in CBP‐resistant RB cells."
39497328
reach
"Inhibiting JNK1/2 activation significantly inhibited USP49 knockdown induced the cell viability inhibition, apoptosis and the JNK1/2 activation in AC16 cardiomyocytes."
30246457
reach
"In consistent with this notion, USP49 knockdown was recently reported to result in a decrease of cell proliferation and invasion as well as an increase of cell apoptosis in glioblastoma cells [ 25 ]."
35367823
USP49 affects FKBP5
8 1 |
USP49 binds FKBP5.
8 |
USP49 binds FKBP5. 8 / 8
8 |
biogrid
No evidence text available
28363942
biogrid
No evidence text available
28363942
biogrid
No evidence text available
28363942
biogrid
No evidence text available
28363942
biogrid
No evidence text available
34431227
biogrid
No evidence text available
34431227
biogrid
No evidence text available
19615732
biogrid
No evidence text available
28363942
USP49 deubiquitinates FKBP5.
1 |
USP49 deubiquitinates FKBP5. 1 / 1
1 |
ubibrowser
"Mechanistically, <span class="match term0">USP49</span> deubiquitinates and stabilizes <span class="match term1">FKBP51</span>, which in turn enhances PHLPP's capability to dephosphorylate AKT Furthermore, <span class="match term0">USP49</span> inhibited pancreatic cancer cell proliferation and enhanced cellular response to gemcitabine in a <span class="match term1">FKBP51</span>-AKT-dependent manner."
28363942
USP49 affects DUSP1
2 1 | 5 1
USP49 binds DUSP1.
2 | 1 1
USP49 binds DUSP1. 4 / 4
2 | 1 1
reach
"The direct interaction between USP49 and DUSP1 was measured by coimmunoprecipitation and ubiquitination analysis."
30246457
sparser
"The direct interaction between USP49 and DUSP1 was measured by coimmunoprecipitation and ubiquitination analysis."
30246457
biogrid
No evidence text available
30246457
biogrid
No evidence text available
30246457
USP49 increases the amount of DUSP1.
| 2
USP49 increases the amount of DUSP1. 2 / 2
| 2
reach
"USP49 positively regulates DUSP1 expression through deubiquitinating DUSP1 in AC16 cardiomyocytes and I/R-induced mouse model."
40102846
reach
"Moreover, USP49 positively regulated DUSP1 expression through deubiquitinating DUSP1."
30246457
USP49 deubiquitinates DUSP1.
1 | 1
USP49 deubiquitinates DUSP1. 2 / 2
1 | 1
reach
"USP49 positively regulates DUSP1 expression through deubiquitinating DUSP1 in AC16 cardiomyocytes and I/R-induced mouse model."
40102846
ubibrowser
"Moreover, <span class="match term0">USP49</span> positively regulated <span class="match term1">DUSP1</span> expression through deubiquitinating <span class="match term1">DUSP1</span>."
30246457
USP49 inhibits DUSP1.
| 1
USP49 inhibits DUSP1. 1 / 1
| 1
reach
"Besides, USP49 inhibits I/R‐induced cell viability suppression and apoptosis in AC16 cells via the DUSP1‐JNK1/2 pathways (Zhang et al., 2019)."
34694031
IGF2BP3 affects USP49
| 8 1
IGF2BP3 increases the amount of USP49.
| 5
IGF2BP3 increases the amount of USP49. 5 / 5
| 5
reach
"Besides, Me‐RIP analysis demonstrated that IGF2BP3 overexpression significantly enhanced the m A methylation level of USP49, whereas IGF2BP3 knockdown reduced the m A methylation of USP49 (Figure 7K)."
39497328
reach
"In addition, we identified insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3)-mediated N6-methyladenosine (m 6 A) modification of USP49 as a regulatory mechanism, wherein IGF2BP3 upregulated USP49 expression in an m 6 A-dependent manner."
39497328
reach
"Altogether, SIRT1 is required for USP49‐mediated malignant proliferation and CBP resistance in RB in vitro.3.7 IGF2BP3 upregulates USP49 expression in an m 6 A-dependent manner."
39497328
reach
"By using MeRIP‐qPCR and RIP‐qPCR assays, we demonstrated that IGF2BP3 upregulated USP49 expression by enhancing the m A level of USP49."
39497328
reach
"It was shown that IGF2BP3 overexpression significantly increased USP49 mRNA levels; conversely, IGF2BP3 deficiency exerted the opposite effects (Figure 7J)."
39497328
IGF2BP3 binds USP49.
| 1 1
IGF2BP3 binds USP49. 2 / 2
| 1 1
reach
"RIP‐qPCR assay showed that the IGF2BP3 specific antibody (IP) was significantly enriched with USP49 mRNA (Figure 7I), confirming the interaction between IGF2BP3 and USP49."
39497328
sparser
"RIP‐qPCR assay showed that the IGF2BP3 specific antibody (IP) was significantly enriched with USP49 mRNA (Figure  xref ), confirming the interaction between IGF2BP3 and USP49."
39497328
IGF2BP3 methylates USP49.
| 1
IGF2BP3 leads to the methylation of USP49. 1 / 1
| 1
reach
"Besides, Me‐RIP analysis demonstrated that IGF2BP3 overexpression significantly enhanced the m A methylation level of USP49, whereas IGF2BP3 knockdown reduced the m A methylation of USP49 (Figure 7K)."
39497328
IGF2BP3 activates USP49.
| 1
IGF2BP3 activates USP49. 1 / 1
| 1
reach
"In addition, we explored including the N6‐methyladenosine (m A) modification of USP49 mediated by insulin‐like growth factor 2 mRNA‐binding protein 3 (IGF2BP3) and the impact of USP49 on the stabilization of sirtuin 1 (SIRT1)."
39497328
FBXO45 affects USP49
1 | 3 5
FBXO45 ubiquitinates USP49.
| 2 3
FBXO45 ubiquitinates USP49. 5 / 5
| 2 3
reach
"FBXO28 contributes to the proliferation of PDAC by promoting ubiquitin-mediated degradation of SMARCC2 (Liu et al., 2023a), and FBXO45 facilitates cell proliferation by ubiquitinating USP49 through binding to its SPRY domain (Wu et al., 2022a)."
38174069
sparser
"Additionally, NEK6 has been reported to enhance the ubiquitination and degradation of USP49 by FBXO45 in pancreatic cancer cells [ xref ]."
40076620
sparser
"Furthermore, our results also demonstrated that Fbxo45 promoted USP49 ubiquitination (Fig. xref and Supplementary Fig. xref )."
35279684
sparser
"Fbxo45-induced USP49 ubiquitination and degradation depend on its SPRY domain."
35279684
reach
"Additionally, NEK6 has been reported to enhance the ubiquitination and degradation of USP49 by FBXO45 in pancreatic cancer cells [64]."
40076620
FBXO45 binds USP49.
1 | 2
FBXO45 binds USP49. 3 / 3
1 | 2
sparser
"Moreover, coIP assays showed that Fbxo45 specifically interacted with USP49 in Panc-1 cells and 293 T cells (Fig. xref and Supplementary Fig. S xref )."
35279684
biogrid
No evidence text available
35279684
sparser
"More importantly, compared with WT, USP49 del significantly reduced the USP49 interaction with Fbxo45 (Fig. xref and Supplementary Fig. xref )."
35279684
FBXO45 deubiquitinates USP49.
| 1
FBXO45 deubiquitinates USP49. 1 / 1
| 1
reach
"Additionally, NEK6 has been reported to enhance the ubiquitination and degradation of USP49 by FBXO45 in pancreatic cancer cells [64]."
40076620
USP49 affects Flag
| 8
USP49 binds Flag. 6 / 6
| 6
sparser
"USP49 Flag-bound immunoprecipitates from these cells were resolved by SDS-PAGE and visualized by silver staining ( Fig. 4 A)."
35367823
sparser
"Co-immunoprecipatation (co-IP) assay identified the interaction between USP49-BAG2 in 293T cells expressing Flag-USP49 and HA-BAG2 ( Fig. 4 D)."
35367823
sparser
"To test this hypothesis, we overexpressed Flag-USP49 in 293T cells and analyzed the endogenous BAG2 protein levels."
35367823
sparser
"Plasmids Flag-USP49, USP49 sh#1 and USP49 sh#2 were constructed and used in our previous study [ 13 ]."
35367823
sparser
"For sample preparations, total proteins extracted from HCT116 cells with or without overexpression of Flag-USP49, were subjected to immunoprecipitation using antibodies against Flag-tag."
35367823
sparser
"To identify the binding partners of USP49, we generated the HCT116 stable cell line ectopically expressing Flag-USP49."
35367823
TP53 binds GST, USP49, and Flag. 1 / 1
| 1
sparser
"This interaction was further confirmed in a GST pull-down assay, with GST-p53 specifically interacting with transfected Flag-USP49, as shown in Fig.  xref ."
29748582
sparser
"We therefore performed a co-immunoprecipitation experiment in which Flag-USP49 and HA-p53 plasmids were separately transfected or co-transfected into 293T cells and potential interactions between Flag-USP49 and HA-p53 were examined (Fig.  xref )."
29748582
FKBP5 affects USP49
8 |
USP49 binds FKBP5. 8 / 8
8 |
biogrid
No evidence text available
28363942
biogrid
No evidence text available
28363942
biogrid
No evidence text available
28363942
biogrid
No evidence text available
28363942
biogrid
No evidence text available
34431227
biogrid
No evidence text available
34431227
biogrid
No evidence text available
19615732
biogrid
No evidence text available
28363942
Valproic acid affects USP49
7 |
Valproic acid decreases the amount of USP49.
6 |
Valproic acid decreases the amount of USP49. 6 / 6
6 |
ctd
No evidence text available
28001369
ctd
No evidence text available
26272509
ctd
No evidence text available
24935251
ctd
No evidence text available
24383497
ctd
No evidence text available
23179753
ctd
No evidence text available
27188386
Valproic acid increases the amount of USP49.
1 |
Valproic acid increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
17183730
USP49 affects Neoplasms
| 1 6
USP49 activates Neoplasms.
| 1 5
USP49 activates Neoplasms. 6 / 6
| 1 5
reach
"In sum, these results demonstrate that USP49 enhances malignant proliferation and facilitates CBP resistance of RB cells.3.3 USP49 accelerates xenograft tumor growth and induces CBP resistance in vivo."
39497328
reach
"Moreover, USP49 knockdown also suppressed tumor and CBP resistance in vivo."
39497328
reach
"It was shown that USP49 silencing significantly attenuated xenograft tumor growth and impaired resistance to CBP (Figure 3A–C)."
39497328
reach
"USP49 mediates tumor progression and poor prognosis through a YAP1-dependent feedback loop in gastric cancer."
35318441
reach
"Furthermore, USP49 accelerated tumor growth and induced CBP resistance in vivo."
39497328
eidos
"USP49 mediates tumor progression and poor prognosis through a YAP1-dependent feedback loop in gastric cancer ."
35318441
USP49 inhibits Neoplasms.
| 1
USP49 inhibits Neoplasms. 1 / 1
| 1
reach
"Xenograft mouse experiments demonstrated that ectopic expression of Fbxo45 enhanced tumor growth in mice and that USP49 overexpression inhibited tumor growth in vivo."
35279684
USP49 affects Histone_H2B
| 7
USP49 deubiquitinates Histone_H2B.
| 6
USP49 deubiquitinates Histone_H2B. 6 / 6
| 6
reach
"Here we report the purification of ubiquitin specific peptidase 49 (USP49) as a histone H2B specific deubiquitinase and demonstrate that H2B deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons."
23824326
reach
"It has been reported that USP49 deubiquitinates histone H2B and regulates co-transcriptional pre-mRNA splicing; interestingly, p53 is also mainly localized to the nucleus."
29748582
reach
"USP49 forms a complex with RuvB-like1 (RVB1) and SUG1 and specifically deubiquitinates histone H2B in vitro and in vivo."
23824326
reach
"Usp49 in complex with RVB1 and SUG1 yeast homologues deubiquitinates H2B, this modification is required for efficient co-transcriptional splicing of a large set of exons."
25962961
reach
"USP49 deubiquitinates histone H2B and regulates cotranscriptional pre-mRNA splicing."
23824326
reach
"Silencing Usp49 induces relatively small changes in gene expression, however alterations in H2B ubiquitination levels caused by Usp49 regulate U1A and U2B association with chromatin and binding to nascent pre-mRNA."
25962961
USP49 decreases the amount of Histone_H2B.
| 1
USP49 decreases the amount of Histone_H2B. 1 / 1
| 1
reach
"Silencing Usp49 induces relatively small changes in gene expression, however alterations in H2B ubiquitination levels caused by Usp49 regulate U1A and U2B association with chromatin and binding to nascent pre-mRNA."
25962961
USP49 affects FKBP4
1 | 6
USP49 deubiquitinates FKBP4.
1 | 5
USP49 deubiquitinates FKBP4. 6 / 6
1 | 5
reach
"Recent studies show that DUBs play essential roles in proliferation, motility, and chemoresistance of malignant cells through stabilizing critical proteins.5 For example, USP49 deubiquitinates FKBP51, which serves as a scaffold for dephosphorylating Ser473 residue at Akt, and further sensitizes pancreatic cancer cells to gemcitabine."
30296012
reach
"USP49 deubiquitinates and stabilizes FKBP51, which in turn induces dephosphorylation of AKT mediated by PHLPP recruitment [165] ."
31604074
reach
"USP49 is a positive regulator of phosphorylation of AKT by deubiquitinating and stabilising FKBP51 which in turn enhances PHLPP ( Luo et al., 2017 )."
32622856
reach
"Mechanistically, USP49 deubiquitinates and stabilizes FKBP51, which in turn enhances PHLPP 's capability to dephosphorylate AKT Furthermore, USP49 inhibited pancreatic cancer cell proliferation and enhanced cellular response to gemcitabine in a FKBP51-AKT-dependent manner."
28363942
reach
"USP49 was shown to inhibit pancreatic cancer cell proliferation and enhance cellular response to gemcitabine by deubiquitinating and stabilizing FKBP51, which in turn enhances PHLPP's capability to de[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35367823
ubibrowser
"Mechanistically, <span class="match term0">USP49</span> deubiquitinates and stabilizes <span class="match term1">FKBP51</span>, which in turn enhances PHLPP's capability to dephosphorylate AKT Furthermore, <span class="match term0">USP49</span> inhibited pancreatic cancer cell proliferation and enhanced cellular response to gemcitabine in a <span class="match term1">FKBP51</span>-AKT-dependent manner."
28363942
USP49 activates FKBP4.
| 1
USP49 activates FKBP4. 1 / 1
| 1
reach
"USP49 directly increases the stability of FKBP51, a scaffolding protein in the interaction between AKT and PHLPP, and thus enhances sensitivity to gemcitabine."
35760282
USP49 affects CREBBP
| 7
USP49 activates CREBBP. 7 / 7
| 7
reach
"Moreover, USP49 knockdown also suppressed tumor and CBP resistance in vivo."
39497328
reach
"Therefore, USP49 promotes RB tumor growth and CBP resistance in vivo.3.4 USP49 promotes cell proliferation and CBP resistance in RB cells via autophagy activation."
39497328
reach
"Furthermore, we provide insights into the underlying mechanism by which USP49 mediates CBP resistance in RB through autophagy activation."
39497328
reach
"Rescue experiments confirmed the pivotal role of SIRT1 in USP49-mediated CBP resistance."
39497328
reach
"Interestingly, USP49 expression was elevated in CBP‐resistant RB cells compared to parental RB cells."
39497328
reach
"Overexpression of USP49 promoted cell proliferation, inhibited cell apoptosis, and increased the IC50 value of CBP in RB cells."
39497328
reach
"On the contrary, USP49 knockdown suppressed cell proliferation, increased cell apoptosis, and reduced the IC50 value of CBP in CBP‐resistant RB cells."
39497328
PAX9 affects USP49
3 | 3 1
PAX9 binds USP49. 7 / 7
3 | 3 1
reach
"Likewise, N-terminus USP49 showed similar ALP staining and intracellular ALP activity as mock-control (Fig. 5L, M), indicating that the interaction between USP49 and PAX9 or MSX1 is essential for odontogenesis."
35273362
reach
"The latter suggests that the interaction between USP49 and PAX9 or MSX1 is essential for normal odontogenesis (Fig. 5)."
35273362
sparser
"Additionally, we demonstrated USP49-PAX9 and USP49-MSX1 interactions using the Duolink PLA assay (Fig.  xref )."
35273362
biogrid
No evidence text available
35273362
biogrid
No evidence text available
35273362
reach
"We identify and demonstrate that USP49 interacts with and deubiquitinates PAX9 and MSX1, thereby extending their protein half-lives."
35273362
biogrid
No evidence text available
35273362
USP49 affects CETN3
5 | 1
USP49 binds CETN3. 6 / 6
5 | 1
biogrid
No evidence text available
19615732
sparser
"We found that both USP49 mutant isoforms were unable to co-precipitate with CETN1 and CETN2 (Fig. xref , upper and middle panel), whereas there was not any apparent reduction in binding of USP49 mutants to CETN3 (Fig. xref , lower panel)."
36702832
biogrid
No evidence text available
36702832
biogrid
No evidence text available
36702832
biogrid
No evidence text available
35367823
biogrid
No evidence text available
33961781
SIRT1 affects USP49
| 4 2
SIRT1 binds USP49.
| 2 2
USP49 binds SIRT1. 4 / 4
| 2 2
sparser
"We first confirmed the interaction between USP49 and SIRT1 by Co‐IP."
39497328
sparser
"Taken together, these results suggested that USP49 interacted with SIRT1 and inhibited SIRT1 ubiquitination and degradation in RB."
39497328
reach
"As shown in Figure 5C,D, USP49 co‐immunoprecipitated with SIRT1, suggesting an interaction between USP49 and SIRT1 in Y‐79/CBP cells."
39497328
reach
"We first confirmed the interaction between USP49 and SIRT1 by Co‐IP."
39497328
SIRT1 inhibits USP49.
| 1
SIRT1 inhibits USP49. 1 / 1
| 1
reach
"36 Here, SIRT1 overexpression restored SIRT1 expression in USP49‐silenced CBP‐resistant RB cells and reversed the inhibitory effects of USP49 knockdown on CBP resistance, cell proliferation, and autophagy."
39497328
SIRT1 activates USP49.
| 1
SIRT1 activates USP49. 1 / 1
| 1
reach
"36 Here, SIRT1 overexpression restored SIRT1 expression in USP49‐silenced CBP‐resistant RB cells and reversed the inhibitory effects of USP49 knockdown on CBP resistance, cell proliferation, and autophagy."
39497328
MYC affects USP49
| 1 3 2
MYC activates USP49.
| 1 2 2
MYC activates USP49. 5 / 5
| 1 2 2
reach
"Moreover, it has been recently proposed that USP49 is transcriptionally activated by c-Myc in colorectal cancer cells [8]."
36702832
sparser
"These findings suggested that USP49 was transcriptionally activated by c-MYC in CRC cells."
35367823
sparser
"Moreover, it has been recently proposed that USP49 is transcriptionally activated by c-Myc in colorectal cancer cells [ xref ]."
36702832
eidos
"Here we revealed that Ubiquitin Specific Peptidase 49 ( USP49 ) is transcriptionally activated by c-MYC in colorectal cancer ( CRC ) , and CRC patients with elevated USP49 levels exhibited significantly shorter survival ."
35367823
reach
"These findings suggested that USP49 was transcriptionally activated by c-MYC in CRC cells.To validate the role of USP49 in CRC progression, we depleted endogenous USP49 in HCT116 and SW48 cells by two[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35367823
MYC increases the amount of USP49.
| 1
MYC increases the amount of USP49. 1 / 1
| 1
reach
"Furthermore, depletion of c-MYC by specific shRNAs significantly suppressed the expression of USP49 in the CRC cell line HCT116 ( Fig. 2 D)."
35367823
MSX1 affects USP49
3 | 2 1
MSX1 binds USP49. 6 / 6
3 | 2 1
reach
"The latter suggests that the interaction between USP49 and PAX9 or MSX1 is essential for normal odontogenesis (Fig. 5)."
35273362
biogrid
No evidence text available
35273362
sparser
"Additionally, we demonstrated USP49-PAX9 and USP49-MSX1 interactions using the Duolink PLA assay (Fig.  xref )."
35273362
biogrid
No evidence text available
35273362
reach
"Likewise, N-terminus USP49 showed similar ALP staining and intracellular ALP activity as mock-control (Fig. 5L, M), indicating that the interaction between USP49 and PAX9 or MSX1 is essential for odontogenesis."
35273362
biogrid
No evidence text available
35273362
CETN3 affects USP49
5 | 1
USP49 binds CETN3. 6 / 6
5 | 1
biogrid
No evidence text available
19615732
sparser
"We found that both USP49 mutant isoforms were unable to co-precipitate with CETN1 and CETN2 (Fig. xref , upper and middle panel), whereas there was not any apparent reduction in binding of USP49 mutants to CETN3 (Fig. xref , lower panel)."
36702832
biogrid
No evidence text available
36702832
biogrid
No evidence text available
36702832
biogrid
No evidence text available
35367823
biogrid
No evidence text available
33961781
USP49 affects autophagy
| 5
USP49 activates autophagy. 5 / 5
| 5
reach
"31 Inhibiting autophagy is still a traditional strategy to overcome CBP resistance in RB, 15 and our study demonstrates that USP49 modulates key autophagy‐related proteins to enhance autophagy, thereby promoting RB cell survival and conferring CBP resistance."
39497328
reach
"Furthermore, it was also revealed that IGF2BP3‐dependent m6A modification of USP49 enhances autophagy by stabilizing SIRT1 via its deubiquitination, helping RB cells survive under CBP treatment."
39497328
reach
"As autophagy activation is implicated in CBP resistance in RB, 15 we explored whether USP49 might promote CBP resistance in RB by inducing autophagy."
39497328
reach
"IGF2BP3-dependent N6-methyladenosine modification of USP49 promotes carboplatin resistance in retinoblastoma by enhancing autophagy via regulating the stabilization of SIRT1 ."
39497328
reach
"Our findings delineate a novel molecular network involving USP49-mediated autophagy in promoting CBP resistance in RB, offering potential targets for therapeutic intervention to enhance treatment efficacy and improve outcomes for RB patients."
39497328
USP49 affects USP44
4 | 1
USP49 binds USP44. 4 / 4
4 |
biogrid
No evidence text available
36702832
biogrid
No evidence text available
19615732
biogrid
No evidence text available
36702832
biogrid
No evidence text available
32076268
USP25 binds USP44, USP49, USP7, and SOX2. 1 / 1
| 1
sparser
"USP25, USP44, USP49, and USP7 bind to the Sox2 promoter, while USP10, USP16, USP3, USP37, USP44, and USP7 bind to the Nanog promoter."
26880980
USP49 affects RUVBL1
2 | 1 1
USP49 binds RUVBL1. 3 / 4
2 | 1
biogrid
No evidence text available
23824326
biogrid
No evidence text available
23824326
reach
"USP49 forms a complex with RuvB-like1 (RVB1) and SUG1 and specifically deubiquitinates histone H2B in vitro and in vivo."
23824326
USP49 binds RUVBL1 and PSMC5. 1 / 1
| 1
sparser
"USP49 forms a complex with RuvB-like1 (RVB1) and SUG1 and specifically deubiquitinates histone H2B in vitro and in vivo."
23824326
USP49 affects PSMC5
2 | 1 1
USP49 binds PSMC5. 3 / 4
2 | 1
reach
"USP49 forms a complex with RuvB-like1 (RVB1) and SUG1 and specifically deubiquitinates histone H2B in vitro and in vivo."
23824326
biogrid
No evidence text available
23824326
biogrid
No evidence text available
23824326
USP49 binds RUVBL1 and PSMC5. 1 / 1
| 1
sparser
"USP49 forms a complex with RuvB-like1 (RVB1) and SUG1 and specifically deubiquitinates histone H2B in vitro and in vivo."
23824326
USP49 affects PI3K
| 5
USP49 inhibits PI3K.
| 4
USP49 inhibits PI3K. 4 / 4
| 4
reach
"In mechanism, overexpression of USP49 inhibited PI3K and AKT signaling, but knockdown of USP49 enhanced this signaling."
31001918
reach
"USP49 was also reported to inhibit non-small cell lung cancer cell growth by suppressing PI3K/AKT signaling ."
34075026
reach
"Further studies indicated that USP49 deubiquitinated PTEN and stabilized PTEN protein, which suggested that USP49 inhibited PI3K and AKT signaling by stabilizing PTEN in NSCLC cells."
31001918
reach
"As shown in Fig. 6D, overexpression of USP49 significantly inhibited cell proliferation and reversed the promotion effect of miR-5000-3p on cell proliferation ability.Due to USP49 was involved in the PI3K/AKT signaling pathway ."
34075026
USP49 activates PI3K.
| 1
USP49 activates PI3K. 1 / 1
| 1
reach
"In conclusion, we demonstrated that USP49 was functional in NSCLC cells, and inhibited NSCLC cell growth by suppressing PI3K and AKT signaling, suggesting that USP49 could be as a novel target for NSCLC therapy."
31001918
USP49 affects NR3C1
2 | 2 1
USP49 binds NR3C1.
2 | 1
NR3C1 binds USP49. 3 / 3
2 | 1
biogrid
No evidence text available
34610959
sparser
"USP49 binds ubiquitinated GR β and decreases its ubiquitination."
34610959
biogrid
No evidence text available
34610959
USP49 increases the amount of NR3C1.
| 1
USP49 increases the amount of NR3C1. 1 / 1
| 1
reach
"Subsequently, we examined whether USP49 could modulates the ubiquitination level of GRβ in GBM cells directly."
34610959
USP49 activates NR3C1.
| 1
USP49 activates NR3C1. 1 / 1
| 1
reach
"As shown in Fig. 4F, the ubiquitination of GRβ was increased upon USP49 knockdown, which correspondingly induced a decrease of GRβ in total lysate (left panel)."
34610959
USP49 affects Aneuploidy
| 5
USP49 inhibits Aneuploidy.
| 3
USP49 inhibits Aneuploidy. 3 / 3
| 3
reach
"USP49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy."
36702832
reach
"Accordingly, further studies will be needed to better understand how USP49-USP44-CETN interaction affects mitotic progression.In summary, our findings show that USP49 regulates the mitotic spindle checkpoint and prevents aneuploidy."
36702832
reach
"USP49 loss impairs cell proliferation and leads to aneuploidy."
36702832
USP49 activates Aneuploidy.
| 2
USP49 activates Aneuploidy. 2 / 2
| 2
reach
"We show that loss of USP49 in different cancer cell lines impairs proliferation and increases aneuploidy."
36702832
reach
"However, we found that USP49 downregulation caused a significant increase in aneuploidy (Fig. 2B), suggesting that passage through mitosis in the absence of USP49 would lead to chromosome mis-segregation."
36702832
USP44 affects USP49
4 | 1
USP49 binds USP44. 4 / 4
4 |
biogrid
No evidence text available
36702832
biogrid
No evidence text available
19615732
biogrid
No evidence text available
36702832
biogrid
No evidence text available
32076268
USP25 binds USP44, USP49, USP7, and SOX2. 1 / 1
| 1
sparser
"USP25, USP44, USP49, and USP7 bind to the Sox2 promoter, while USP10, USP16, USP3, USP37, USP44, and USP7 bind to the Nanog promoter."
26880980
RUVBL1 affects USP49
2 | 1 1
USP49 binds RUVBL1. 3 / 4
2 | 1
biogrid
No evidence text available
23824326
biogrid
No evidence text available
23824326
reach
"USP49 forms a complex with RuvB-like1 (RVB1) and SUG1 and specifically deubiquitinates histone H2B in vitro and in vivo."
23824326
USP49 binds RUVBL1 and PSMC5. 1 / 1
| 1
sparser
"USP49 forms a complex with RuvB-like1 (RVB1) and SUG1 and specifically deubiquitinates histone H2B in vitro and in vivo."
23824326
PSMC5 affects USP49
2 | 1 1
USP49 binds PSMC5. 3 / 4
2 | 1
reach
"USP49 forms a complex with RuvB-like1 (RVB1) and SUG1 and specifically deubiquitinates histone H2B in vitro and in vivo."
23824326
biogrid
No evidence text available
23824326
biogrid
No evidence text available
23824326
USP49 binds RUVBL1 and PSMC5. 1 / 1
| 1
sparser
"USP49 forms a complex with RuvB-like1 (RVB1) and SUG1 and specifically deubiquitinates histone H2B in vitro and in vivo."
23824326
BAG2 affects USP49
3 | 1 1
USP49 binds BAG2. 5 / 5
3 | 1 1
reach
"Moreover, co-immunoprecipitation using HCT116 cell lysates validated this specific interaction between endogenous BAG2 and USP49 in CRC cells ( Fig. 4 E)."
35367823
sparser
"Co-immunoprecipatation (co-IP) assay identified the interaction between USP49-BAG2 in 293T cells expressing Flag-USP49 and HA-BAG2 ( Fig. 4 D)."
35367823
biogrid
No evidence text available
35367823
biogrid
No evidence text available
35367823
biogrid
No evidence text available
35367823
Bisphenol A affects USP49
4 |
Bisphenol A decreases the amount of USP49.
2 |
Bisphenol A decreases the amount of USP49. 2 / 2
2 |
ctd
No evidence text available
25181051
ctd
No evidence text available
33221593
Bisphenol A methylates USP49.
1 |
Bisphenol A methylates USP49. 1 / 1
1 |
ctd
No evidence text available
30906313
Bisphenol A increases the amount of USP49.
1 |
Bisphenol A increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
30248606
USP49 affects gemcitabine
| 4
USP49 activates gemcitabine.
| 3
USP49 activates gemcitabine. 3 / 3
| 3
reach
"USP49 directly increases the stability of FKBP51, a scaffolding protein in the interaction between AKT and PHLPP, and thus enhances sensitivity to gemcitabine."
35760282
reach
"Mechanistically, USP49 deubiquitinates and stabilizes FKBP51, which in turn enhances PHLPP 's capability to dephosphorylate AKT Furthermore, USP49 inhibited pancreatic cancer cell proliferation and enhanced cellular response to gemcitabine in a FKBP51-AKT-dependent manner."
28363942
reach
"Luo et al. [XREF_BIBR] found that FKBP51 could promote PHLPP-Akt interaction and following Akt dephosphorylation at ser473, so that USP49 enhanced cellular response to gemcitabine through FKBP51-Akt signaling."
28938696
USP49 deubiquitinates gemcitabine.
| 1
USP49 deubiquitinates gemcitabine. 1 / 1
| 1
reach
"Recent studies show that DUBs play essential roles in proliferation, motility, and chemoresistance of malignant cells through stabilizing critical proteins.5 For example, USP49 deubiquitinates FKBP51, which serves as a scaffold for dephosphorylating Ser473 residue at Akt, and further sensitizes pancreatic cancer cells to gemcitabine."
30296012
USP49 affects TP53BP1
| 4
USP49 inhibits TP53BP1.
| 3
USP49 inhibits TP53BP1. 3 / 3
| 3
reach
"USP49 overexpression suppressed ubiquitylation of γH2AX and DSB-induced foci formation of 53BP1."
35897775
reach
"Namely, inhibition or deletion of 53BP1 resulted in increased HR ( Chen, 2020; Xie, 2007; Canny, 2018 ), which is consistent with our results showing that over-expression of USP49 suppressed 53BP1 loa[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35598681
reach
"But once DSBs were generated, 53BP1 loading on chromatin and NHEJ could be promoted by proteasomal degradation of USP49."
35598681
USP49 activates TP53BP1.
| 1
USP49 activates TP53BP1. 1 / 1
| 1
reach
"Thus, we studied the effect of USP49 over-expression on DSB-induced foci formation of 53BP1 with a cell line stably expressing mCherry-53BP1."
35598681
USP49 affects PTEN
1 | 3
USP49 inhibits PTEN.
| 2
USP49 inhibits PTEN. 2 / 2
| 2
reach
"By stabilizing PTEN, USP49 inhibits non–small cell lung cancer growth (35)."
34610959
reach
"Further studies indicated that USP49 deubiquitinated PTEN and stabilized PTEN protein, which suggested that USP49 inhibited PI3K and AKT signaling by stabilizing PTEN in NSCLC cells."
31001918
USP49 deubiquitinates PTEN.
1 | 1
USP49 deubiquitinates PTEN. 2 / 2
1 | 1
ubibrowser
"Further studies indicated that <span class="match term0">USP49</span> deubiquitinated <span class="match term1">PTEN</span> and stabilized <span class="match term1">PTEN</span> protein, which suggested that <span class="match term0">USP49</span> inhibited PI3K/AKT signaling by stabilizing <span class="match term1">PTEN</span> in NSCLC cells"
31001918
reach
"Further studies indicated that USP49 deubiquitinated PTEN and stabilized PTEN protein, which suggested that USP49 inhibited PI3K and AKT signaling by stabilizing PTEN in NSCLC cells."
31001918
USP49 affects Cell Survival
| 4
USP49 activates Cell Survival.
| 3
USP49 activates Cell Survival. 3 / 3
| 3
reach
"To the best of our knowledge, this is the first study that identified deubiquitinase regulating BAG2.In summary, we revealed an oncogenic role of USP49 in CRC cells and identified a novel mechanism by[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35367823
reach
"Besides, USP49 inhibits I/R‐induced cell viability suppression and apoptosis in AC16 cells via the DUSP1‐JNK1/2 pathways (Zhang et al., 2019)."
34694031
reach
"USP49 inhibits ischemia-reperfusion-induced cell viability suppression and apoptosis in human AC16 cardiomyocytes through DUSP1-JNK1/2 signaling."
30246457
USP49 inhibits Cell Survival.
| 1
USP49 inhibits Cell Survival. 1 / 1
| 1
reach
"Inhibiting JNK1/2 activation significantly inhibited USP49 knockdown induced the cell viability inhibition, apoptosis and the JNK1/2 activation in AC16 cardiomyocytes."
30246457
USP49 affects AKT
| 4
USP49 inhibits AKT.
| 3
USP49 inhibits AKT. 3 / 3
| 3
reach
"In mechanism, overexpression of USP49 inhibited PI3K and AKT signaling, but knockdown of USP49 enhanced this signaling."
31001918
reach
"Further studies indicated that USP49 deubiquitinated PTEN and stabilized PTEN protein, which suggested that USP49 inhibited PI3K and AKT signaling by stabilizing PTEN in NSCLC cells."
31001918
reach
"In contrast, another DUB, USP49, inhibits AKT pathway by promoting interaction of PHLPP1 to AKT, thus facilitating the de-phosphorylation of AKT."
28923280
USP49 activates AKT.
| 1
USP49 activates AKT. 1 / 1
| 1
reach
"In conclusion, we demonstrated that USP49 was functional in NSCLC cells, and inhibited NSCLC cell growth by suppressing PI3K and AKT signaling, suggesting that USP49 could be as a novel target for NSCLC therapy."
31001918
TFDP1 affects USP49
4 |
TFDP1 decreases the amount of USP49. 4 / 4
4 |
biopax:msigdb
No evidence text available
biopax:msigdb
No evidence text available
biopax:msigdb
No evidence text available
biopax:msigdb
No evidence text available
NR3C1 affects USP49
2 | 1 1
NR3C1 binds USP49.
2 | 1
NR3C1 binds USP49. 3 / 3
2 | 1
biogrid
No evidence text available
34610959
sparser
"USP49 binds ubiquitinated GR β and decreases its ubiquitination."
34610959
biogrid
No evidence text available
34610959
NR3C1 inhibits USP49.
| 1
NR3C1 inhibits USP49. 1 / 1
| 1
reach
"Similarly, GRβ overexpression reversed the inhibition of USP49 knockdown (Fig. 5C and D)."
34610959
H2AX affects USP49
1 | 1 2
USP49 binds H2AX. 3 / 3
1 | 2
sparser
"Although recruitment of GFP-USP49 (WT) to DSB sites was not detectable, this may be because of transient interaction between USP49 and a potential substrate such as ubiquitylated-γH2AX."
35598681
sparser
"The colocalization of USP49 (CA) and γH2AX prompted us to investigate the protein–protein interaction between USP49 (CA) and γH2AX ( Fig. 2 C)."
35598681
biogrid
No evidence text available
35598681
USP49 binds H2AX and 2 C. 1 / 1
| 1
reach
"The colocalization of USP49 (CA) and γH2AX prompted us to investigate the protein–protein interaction between USP49 (CA) and γH2AX ( Fig. 2 C)."
35598681
DUSP1 affects USP49
2 | 1 1
USP49 binds DUSP1. 4 / 4
2 | 1 1
reach
"The direct interaction between USP49 and DUSP1 was measured by coimmunoprecipitation and ubiquitination analysis."
30246457
sparser
"The direct interaction between USP49 and DUSP1 was measured by coimmunoprecipitation and ubiquitination analysis."
30246457
biogrid
No evidence text available
30246457
biogrid
No evidence text available
30246457
Trichostatin A affects USP49
3 |
Trichostatin A decreases the amount of USP49. 3 / 3
3 |
ctd
No evidence text available
26272509
ctd
No evidence text available
24935251
ctd
No evidence text available
27188386
USP49 affects cell growth
| 3
USP49 inhibits cell growth. 3 / 3
| 3
reach
"USP49 was also reported to inhibit non-small cell lung cancer cell growth by suppressing PI3K/AKT signaling ."
34075026
reach
"As a result, overexpression of USP49 significantly inhibited cell growth of NSCLC cells."
31001918
reach
"In conclusion, we demonstrated that USP49 was functional in NSCLC cells, and inhibited NSCLC cell growth by suppressing PI3K and AKT signaling, suggesting that USP49 could be as a novel target for NSCLC therapy."
31001918
USP49 activates cell differentiation.
| 2
reach
"We demonstrated that USP49 promoted odontogenic differentiation, as evidenced by increased ALP activity and calcium mineralization in hDPSCs."
35273362
reach
"USP49 promotes the odontogenic differentiation of hDPSCs by stabilizing PAX9 and MSX1."
35273362
USP49 inhibits cell differentiation.
| 1
reach
"In hiPSCs, loss-of-function of the USP49 gene in hiPSCs also caused delayed differentiation into NCLCs (Supplementary Fig. S14A)."
35273362
USP49 affects RPN1
3 |
USP49 binds RPN1. 3 / 3
3 |
biogrid
No evidence text available
19615732
biogrid
No evidence text available
36702832
biogrid
No evidence text available
36702832
USP49 affects RB1
| 3
USP49 activates RB1. 3 / 3
| 3
reach
"These results indicated that USP49 promotes autophagy activation in RB cells."
39497328
reach
"MeRIP‐qPCR analysis showed that the m A level of USP49 mRNA was increased in Y‐79/CBP cells compared with that in Y‐79 cells (Figure 7A), indicating m A modification might be involved in USP49 upregulation in RB resistance to CBP."
39497328
reach
"Therefore, USP49 promotes RB tumor growth and CBP resistance in vivo.3.4 USP49 promotes cell proliferation and CBP resistance in RB cells via autophagy activation."
39497328
USP49 affects Osteoarthritis
| 1 2
USP49 inhibits Osteoarthritis. 3 / 3
| 1 2
reach
"USP49 attenuates OA progression by inhibiting the Wnt/β-catenin pathway."
34216299
eidos
"USP49 attenuates OA progression by inhibiting the Wnt / beta-catenin pathway ."
34216299
reach
"Taken together, our study not only demonstrates that Usp49 can negatively regulate the progression of OA by inhibiting the Wnt and beta-catenin signaling cascade, but also elucidates the underlying molecular mechanisms."
32737772
reach
"USP49 silencing decreases cell proliferation, migration, and invasion and enhances GC cell sensitivity to chemotherapy; however, this effect can be reversed by YAP1 overexpression."
36497313
reach
"The present study showed that USP49 promotes cell proliferation, invasion, and antiapoptosis, suggesting a role as a tumor promoter in GBM."
34610959
reach
"In consistent with this notion, USP49 knockdown was recently reported to result in a decrease of cell proliferation and invasion as well as an increase of cell apoptosis in glioblastoma cells [ 25 ]."
35367823
USP49 affects MDM2
| 2 1
USP49 decreases the amount of MDM2.
| 2
USP49 decreases the amount of MDM2. 2 / 2
| 2
reach
"Importantly, USP49 knockout stimulated MDM2 transcriptional level and then inhibited the mRNA levels of TP53 target genes."
35072515
reach
"Conversely, overexpression of USP49 suppressed MDM2 gene expression and then promoted TP53 target genes."
35072515
USP49 binds MDM2.
| 1
USP49 binds MDM2. 1 / 1
| 1
sparser
"Moreover, chromatin immunoprecipitation revealed that USP49 directly bound to the promoter of MDM2 gene."
35072515
USP49 affects HIV-1 infectivity
| 3
USP49 inhibits HIV-1 infectivity. 3 / 3
| 3
eidos
"3 ) The authors should show that USP49 works with the endogenous A3G to inhibit HIV-1 infectivity ."
31397674
eidos
"Results in the new Figure 2E showed that USP49 worked with endogenous A3G to inhibit HIV-1 infectivity ."
31397674
eidos
"Essential revisions : 1 ) The authors should provide more solid and systematic data to show that USP49 inhibits HIV-1 infectivity through A3G ."
31397674
USP49 affects FBXO45
1 | 2
FBXO45 binds USP49. 3 / 3
1 | 2
sparser
"Moreover, coIP assays showed that Fbxo45 specifically interacted with USP49 in Panc-1 cells and 293 T cells (Fig. xref and Supplementary Fig. S xref )."
35279684
biogrid
No evidence text available
35279684
sparser
"More importantly, compared with WT, USP49 del significantly reduced the USP49 interaction with Fbxo45 (Fig. xref and Supplementary Fig. xref )."
35279684
USP49 affects CTNNB1
1 | 2
USP49 inhibits CTNNB1.
| 1
USP49 inhibits CTNNB1. 1 / 1
| 1
reach
"6-Gingerol (17) upregulates the expression of ubiquitin-specific peptidase 49 (USP49), promotes the deubiquitination of Axin, and increases the degradation of β-catenin, resulting in the inhibition of Wnt/β-catenin signaling and the attenuation of apoptosis in IL-1β-treated rat chondrocytes (Yang L. et al., 2020)."
37324450
USP49 binds CTNNB1.
1 |
USP49 binds CTNNB1. 1 / 1
1 |
biogrid
No evidence text available
30323974
USP49 activates CTNNB1.
| 1
USP49 activates CTNNB1. 1 / 1
| 1
reach
"Taken together, our study not only demonstrates that Usp49 can negatively regulate the progression of OA by inhibiting the Wnt and beta-catenin signaling cascade, but also elucidates the underlying molecular mechanisms."
32737772
USP49 affects CETN2
3 |
USP49 binds CETN2. 3 / 3
3 |
biogrid
No evidence text available
33961781
biogrid
No evidence text available
36702832
biogrid
No evidence text available
19615732
USP49 affects AXIN
| 2 1
USP49 ubiquitinates AXIN.
| 2
Modified USP49 leads to the ubiquitination of AXIN. 1 / 1
| 1
reach
"Treating primary rat chondrocytes with different concentrations of IL-1beta resulted in decreased Usp49 expression, while Usp49 overexpression could attenuate IL-1beta-induced chondrocyte apoptosis by promoting Axin deubiquitination."
32737772
USP49 leads to the ubiquitination of AXIN. 1 / 1
| 1
reach
"USP49 is lowly expressed in human OA chondrocytes and overexpression of USP49 in primary rat chondrocytes inhibits apoptosis by promoting AXIN deubiquitination and reducing the level of IL-1β, a marker for OA progression (Fig. 1) [26]."
34216299
USP49 binds AXIN.
| 1
USP49 binds AXIN. 1 / 1
| 1
sparser
"Mechanistically, USP49 interacts with Axin for deubiquitination, which downregulates Wnt/β-catenin signaling to mitigate chondrocyte apoptosis [69] ."
38583240
RPN1 affects USP49
3 |
USP49 binds RPN1. 3 / 3
3 |
biogrid
No evidence text available
19615732
biogrid
No evidence text available
36702832
biogrid
No evidence text available
36702832
NEK6 affects USP49
| 2 1
NEK6 ubiquitinates USP49.
| 2
NEK6 ubiquitinates USP49. 2 / 2
| 2
reach
"Furthermore, Fbxo45-mediated USP49 ubiquitination and degradation are enhanced by NEK6 kinase."
35279684
reach
"Additionally, NEK6 has been reported to enhance the ubiquitination and degradation of USP49 by FBXO45 in pancreatic cancer cells [64]."
40076620
NEK6 binds USP49.
| 1
NEK6 binds USP49. 1 / 1
| 1
sparser
"However, we found that NEK6 specifically interacted with USP49 but not NEK3, suggesting that NEK6 was critically involved in USP49 degradation (Supplementary Fig. xref )."
35279684
CETN2 affects USP49
3 |
USP49 binds CETN2. 3 / 3
3 |
biogrid
No evidence text available
33961781
biogrid
No evidence text available
36702832
biogrid
No evidence text available
19615732
Vorinostat affects USP49
2 |
Vorinostat decreases the amount of USP49. 2 / 2
2 |
ctd
No evidence text available
27188386
ctd
No evidence text available
26272509
Titanium dioxide affects USP49
2 |
Titanium dioxide methylates USP49.
1 |
ctd
No evidence text available
35295148
Titanium dioxide demethylates USP49.
1 |
Titanium dioxide demethylates USP49. 1 / 1
1 |
ctd
No evidence text available
35295148
Pirinixic acid affects USP49
2 |
Pirinixic acid decreases the amount of USP49.
1 |
Pirinixic acid decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
19710929
Pirinixic acid activates USP49.
1 |
Pirinixic acid activates USP49. 1 / 1
1 |
ctd
No evidence text available
19710929
Phenylmercury acetate decreases the amount of USP49. 2 / 2
2 |
ctd
No evidence text available
26272509
ctd
No evidence text available
27188386
Panobinostat affects USP49
2 |
Panobinostat decreases the amount of USP49. 2 / 2
2 |
ctd
No evidence text available
26272509
ctd
No evidence text available
27188386
MiR-5000-3p affects USP49
| 2
MiR-5000-3p inhibits USP49.
| 1
MiR-5000-3p inhibits USP49. 1 / 1
| 1
reach
"The qRT-PCR data show that the expression of miR-5000-3p decreased in OR-HCT116 cells expressing high USP49 compared with that in control and OE-NC cells (Fig. 6C)."
34075026
MiR-5000-3p decreases the amount of USP49.
| 1
MiR-5000-3p decreases the amount of USP49. 1 / 1
| 1
reach
"As presented in Fig. 6A, the overexpression of miR-5000-3p reduced USP49 level in HCT116 cells while knockdown of miR-5000-3p elevated USP49 expression in OR-CRC cells, respectively."
34075026
Cadmium dichloride increases the amount of USP49. 2 / 2
2 |
ctd
No evidence text available
38568856
ctd
No evidence text available
35301059
Bis(2-ethylhexyl) phthalate activates USP49. 2 / 2
| 2
eidos
"Multiplex RT-PCR and Western blot analysis revealed that USP49 was upregulated by DEHP in A2780 and OVCAR5 cells ."
32143396
eidos
"These findings suggest that USP49 upregulation by DEHP induces cell death via p53 regulation , which subsequently leads to POF due to follicle depletion ."
32143396
YAP1 affects USP49
| 1 1
YAP1 binds USP49.
| 1
YAP1 binds USP49. 1 / 1
| 1
sparser
"Our group has previously studied the Hippo-YAP1 pathway and discovered that the USP49-YAP1 feedback loop promotes GC progression [ xref ]."
38493426
YAP1 activates USP49.
| 1
YAP1 bound to TEAD4 activates USP49. 1 / 1
| 1
reach
"Moreover, USP49 was transcriptionally activated by the YAP1/TEAD4 complex, which formed a positive feedback loop with YAP1 to promote the malignant progression of GC cells."
35318441
USP7 affects USP49
| 1 1
USP49 binds USP7. 1 / 1
| 1
reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
38874156
USP25 binds USP44, USP49, USP7, and SOX2. 1 / 1
| 1
sparser
"USP25, USP44, USP49, and USP7 bind to the Sox2 promoter, while USP10, USP16, USP3, USP37, USP44, and USP7 bind to the Nanog promoter."
26880980
USP49 affects signaling
| 2
USP49 inhibits signaling. 2 / 2
| 2
sparser
"In mechanism, overexpression of USP49 inhibited PI3K/AKT signaling, but knockdown of USP49 enhanced this signaling."
31001918
sparser
"Further studies indicated that USP49 deubiquitinated PTEN and stabilized PTEN protein, which suggested that USP49 inhibited PI3K/AKT signaling by stabilizing PTEN in NSCLC cells."
31001918
USP49 affects proteolysis
| 2
USP49 inhibits proteolysis.
| 1
USP49 inhibits proteolysis. 1 / 1
| 1
reach
"Thus, PAX9 and MSX1 ubiquitination was elevated in USP49 KO-hDPSCs, indicating that the loss of USP49 promotes PAX9 and MSX1 protein degradation."
35273362
USP49 activates proteolysis.
| 1
USP49 activates proteolysis. 1 / 1
| 1
reach
"Importantly, the loss of USP49 promoted the polyubiquitination of PAX9 and MSX1, thereby triggering rapid protein degradation (Fig. 4)."
35273362
USP49 inhibits phosphatidylinositol 3-kinase/protein kinase B signal transduction. 2 / 2
| 2
reach
"USP49 was also reported to inhibit non-small cell lung cancer cell growth by suppressing PI3K/AKT signaling ."
34075026
reach
"As shown in Fig. 6D, overexpression of USP49 significantly inhibited cell proliferation and reversed the promotion effect of miR-5000-3p on cell proliferation ability.Due to USP49 was involved in the PI3K/AKT signaling pathway ."
34075026
USP49 affects miR-5000-3p
| 2
USP49 increases the amount of miR-5000-3p.
| 1
USP49 bound to miR-5000-3p promoter increases the amount of miR-5000-3p. 1 / 1
| 1
reach
"Molecular mechanism assays revealed that USP49 binds to the miR-5000-3p promoter to increase the expression of miR-5000-3p, resulting in cancer cells sensitized to OXA."
34075026
USP49 decreases the amount of miR-5000-3p.
| 1
USP49 decreases the amount of miR-5000-3p. 1 / 1
| 1
reach
"USP49 suppresses miR-5000-3p expression and regulates PI3K/AKT signal pathway."
34075026
USP49 inhibits homologous recombination. 1 / 1
| 1
reach
"To our surprise, over-expression of USP49 (CA), which affected neither γH2AX ubiquitylation nor 53BP1 foci formation, strongly suppressed HR efficiency ( Fig. 3 B)."
35598681
USP49 activates homologous recombination. 1 / 1
| 1
reach
"Over-expression of USP49 (WT) significantly increased the efficiency of HR compared to the control ( Fig. 3 B)."
35598681
USP49 affects ferroptosis
| 2
USP49 activates ferroptosis. 2 / 2
| 2
reach
"Moreover, overexpression of Glutathione PeroXidase 4 (GPX4) reversed the ferroptosis and proliferation inhibition induced by USP49 knockdown."
39234990
reach
"Here, we report that the downregulation of Ubiquitin-specific peptidase 49 (USP49) promotes ferroptosis in OE33 and OE19 cells, thereby inhibiting cell proliferation in vitro and in vivo, whereas the overexpression of USP49 had the opposite effect."
39234990
USP49 affects USP7
| 1 1
USP49 binds USP7. 1 / 1
| 1
reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
38874156
USP25 binds USP44, USP49, USP7, and SOX2. 1 / 1
| 1
sparser
"USP25, USP44, USP49, and USP7 bind to the Sox2 promoter, while USP10, USP16, USP3, USP37, USP44, and USP7 bind to the Nanog promoter."
26880980
USP49 affects USP49
| 2
USP49 increases the amount of USP49.
| 1
USP49 increases the amount of USP49. 1 / 1
| 1
reach
"Our findings revealed that IGF2BP3‐dependent m A modification of USP49 mRNA upregulates USP49 expression to enhance SIRT1 stability.These findings highlight the critical role of USP49 in cellular processes and its potential as a therapeutic target in RB."
39497328
USP49 deubiquitinates USP49.
| 1
USP49 leads to the deubiquitination of USP49. 1 / 1
| 1
reach
"Taken together, these results suggested that USP49 interacted with SIRT1 and inhibited SIRT1 ubiquitination and degradation in RB.3.6 SIRT1 overexpression reverses the effects of USP49 silencing in vitro."
39497328
USP49 affects Stomach Neoplasms
| 2
USP49 activates Stomach Neoplasms. 2 / 2
| 2
reach
"In this study, ubiquitin-specific peptidase 49 (USP49) was identified as a novel deubiquitinase of YAP1, knockdown of USP49 inhibited the proliferation, metastasis, chemoresistance, and peritoneal metastasis of GC cells."
35318441
reach
"Because USP49 is a target gene modulated by YAP1/TEAD4, this result indicates that USP49 and YAP1 form a positive feedback loop to support the malignant progression of GC [67]."
36497313
USP49 affects SHCBP1
1 | 1
USP49 deubiquitinates SHCBP1.
| 1
USP49 deubiquitinates SHCBP1. 1 / 1
| 1
reach
"Mechanistically, USP49 deubiquitinates and stabilizes Shc SH2-domain binding protein 1 (SHCBP1), subsequently facilitating the entry of β-catenin into the nucleus to enhance GPX4 transcriptional expression."
39234990
USP49 binds SHCBP1.
1 |
USP49 binds SHCBP1. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 activates Neoplasm Metastasis. 2 / 2
| 2
reach
"For instance, USP49 promotes the proliferation, metastasis, chemoresistance, and peritoneal metastasis in gastric cancer cells."
39497328
reach
"In this study, ubiquitin-specific peptidase 49 (USP49) was identified as a novel deubiquitinase of YAP1, knockdown of USP49 inhibited the proliferation, metastasis, chemoresistance, and peritoneal metastasis of GC cells."
35318441
USP49 affects MDM2-p53 axis genes
| 2
USP49 increases the amount of MDM2-p53 axis genes.
| 1
USP49 increases the amount of MDM2-p53 axis genes. 1 / 1
| 1
reach
"Therefore, our findings indicated that USP49-mediated H2B deubiquitination controls the transcription of MDM2-p53 axis genes in the process of HCT116 cell proliferation."
35072515
USP49 deubiquitinates MDM2-p53 axis genes.
| 1
USP49 leads to the deubiquitination of MDM2-p53 axis genes. 1 / 1
| 1
reach
"Therefore, our findings indicated that USP49-mediated H2B deubiquitination controls the transcription of MDM2-p53 axis genes in the process of HCT116 cell proliferation."
35072515
USP49 affects JNK
| 2
USP49 activates JNK. 2 / 2
| 2
reach
"Inhibiting JNK1/2 activation significantly inhibited USP49 knockdown induced the cell viability inhibition, apoptosis and the JNK1/2 activation in AC16 cardiomyocytes."
30246457
reach
"USP49 knockdown Inhibits JNK1/2 activation and cell apoptosis [192]."
40102846
USP49 affects IGF2BP3
| 1 1
IGF2BP3 binds USP49. 2 / 2
| 1 1
reach
"RIP‐qPCR assay showed that the IGF2BP3 specific antibody (IP) was significantly enriched with USP49 mRNA (Figure 7I), confirming the interaction between IGF2BP3 and USP49."
39497328
sparser
"RIP‐qPCR assay showed that the IGF2BP3 specific antibody (IP) was significantly enriched with USP49 mRNA (Figure  xref ), confirming the interaction between IGF2BP3 and USP49."
39497328
USP49 affects H2BC21
1 1 |
USP49 deubiquitinates H2BC21.
1 |
USP49 deubiquitinates H2BC21. 1 / 1
1 |
ubibrowser
No evidence text available
USP49 binds H2BC21.
1 |
USP49 binds H2BC21. 1 / 1
1 |
biogrid
No evidence text available
23824326
USP49 affects GRβ
| 2
USP49 binds GRβ. 2 / 2
| 2
sparser
"Through IP analysis, we further confirmed the interaction between USP49 and GRβ, particularly that this interaction was blocked by the mutation of K733R ( xref )."
34610959
sparser
"Furthermore, it confirmed that USP49 binds specifically to GRβ but not GRα ( xref ), suggesting that the binding of USP49 depends specifically on the COOH-terminal ubiquitination state of the GRβ."
34610959
USP49 affects EZH2
2 |
USP49 binds EZH2. 2 / 2
2 |
biogrid
No evidence text available
29669287
biogrid
No evidence text available
29669287
USP49 affects CETN1
2 |
USP49 binds CETN1. 2 / 2
2 |
biogrid
No evidence text available
19615732
biogrid
No evidence text available
36702832
USP49 affects CCAT1
2 |
CCAT1 binds USP49. 2 / 2
2 |
biogrid
No evidence text available
34431227
biogrid
No evidence text available
34431227
USP49 affects BBC3
| 2
Modified USP49 increases the amount of BBC3. 1 / 1
| 1
reach
"Overexpression of USP49 significantly increased p21, BAX, and PUMA mRNA levels, whereas knockdown of USP49 suppressed p21, BAX, and PUMA levels when HCT116 cells were exposed to Eto."
29748582
USP49 increases the amount of BBC3. 1 / 1
| 1
reach
"Overexpression of USP49 significantly increased p21, BAX, and PUMA mRNA levels, whereas knockdown of USP49 suppressed p21, BAX, and PUMA levels when HCT116 cells were exposed to Eto."
29748582
TP53 affects Flag
| 2
TP53 binds GST, USP49, and Flag. 1 / 1
| 1
sparser
"This interaction was further confirmed in a GST pull-down assay, with GST-p53 specifically interacting with transfected Flag-USP49, as shown in Fig.  xref ."
29748582
sparser
"We therefore performed a co-immunoprecipitation experiment in which Flag-USP49 and HA-p53 plasmids were separately transfected or co-transfected into 293T cells and potential interactions between Flag-USP49 and HA-p53 were examined (Fig.  xref )."
29748582
Proteasome affects USP49
| 2
Proteasome inhibits USP49. 2 / 2
| 2
reach
"These data suggest that endogenous USP49 is degraded by a proteasome after DSB induction."
35598681
reach
"Here we showed that endogenous USP49 protein was degraded by proteasome upon induction of DSBs ( Fig. 5 A)."
35598681
LEF1 affects USP49
2 |
LEF1 decreases the amount of USP49. 2 / 2
2 |
biopax:msigdb
No evidence text available
biopax:msigdb
No evidence text available
IL1B affects USP49
| 2
IL1B increases the amount of USP49.
| 1
IL1B increases the amount of USP49. 1 / 1
| 1
reach
"IL-1β stimulation on primary rat chondrocytes caused the downregulation of USP49 expression."
37359559
IL1B decreases the amount of USP49.
| 1
IL1B decreases the amount of USP49. 1 / 1
| 1
reach
"Furthermore, in rat primary chondrocytes, treatment with IL-1β was found to diminish USP49 expression, an enzyme involved in deubiquitinating AXIN1."
38291457
GTF3A affects USP49
2 |
GTF3A decreases the amount of USP49. 2 / 2
2 |
biopax:msigdb
No evidence text available
biopax:msigdb
No evidence text available
GRβ affects USP49
| 2
USP49 binds GRβ. 2 / 2
| 2
sparser
"Through IP analysis, we further confirmed the interaction between USP49 and GRβ, particularly that this interaction was blocked by the mutation of K733R ( xref )."
34610959
sparser
"Furthermore, it confirmed that USP49 binds specifically to GRβ but not GRα ( xref ), suggesting that the binding of USP49 depends specifically on the COOH-terminal ubiquitination state of the GRβ."
34610959
Flag affects TP53
| 2
TP53 binds GST, USP49, and Flag. 1 / 1
| 1
sparser
"This interaction was further confirmed in a GST pull-down assay, with GST-p53 specifically interacting with transfected Flag-USP49, as shown in Fig.  xref ."
29748582
sparser
"We therefore performed a co-immunoprecipitation experiment in which Flag-USP49 and HA-p53 plasmids were separately transfected or co-transfected into 293T cells and potential interactions between Flag-USP49 and HA-p53 were examined (Fig.  xref )."
29748582
EZH2 affects USP49
2 |
USP49 binds EZH2. 2 / 2
2 |
biogrid
No evidence text available
29669287
biogrid
No evidence text available
29669287
E2F1 affects USP49
2 |
E2F1 decreases the amount of USP49. 2 / 2
2 |
biopax:msigdb
No evidence text available
biopax:msigdb
No evidence text available
CREBBP affects USP49
| 2
CREBBP inhibits USP49.
| 1
CREBBP inhibits USP49. 1 / 1
| 1
reach
"In sum, these results demonstrate that USP49 enhances malignant proliferation and facilitates CBP resistance of RB cells.3.3 USP49 accelerates xenograft tumor growth and induces CBP resistance in vivo."
39497328
CREBBP increases the amount of USP49.
| 1
CREBBP increases the amount of USP49. 1 / 1
| 1
reach
"Altogether, SIRT1 is required for USP49‐mediated malignant proliferation and CBP resistance in RB in vitro.3.7 IGF2BP3 upregulates USP49 expression in an m 6 A-dependent manner."
39497328
CETN1 affects USP49
2 |
USP49 binds CETN1. 2 / 2
2 |
biogrid
No evidence text available
19615732
biogrid
No evidence text available
36702832
CCAT1 affects USP49
2 |
CCAT1 binds USP49. 2 / 2
2 |
biogrid
No evidence text available
34431227
biogrid
No evidence text available
34431227
CAP affects USP49
| 2
CAP increases the amount of USP49.
| 1
CAP increases the amount of USP49. 1 / 1
| 1
reach
"In conclusion, based on the finding that CAP enhances the ubiquitinated degradation of HDAC3 by downregulating USP49 expression, we further demonstrated that CAP promotes p53 expression by increasing the lactylation level of H3K18."
| PMC
CAP decreases the amount of USP49.
| 1
CAP decreases the amount of USP49. 1 / 1
| 1
reach
"Subsequent RT-qPCR and western blot assays indicated that CAP significantly decreased USP49 expression (Fig. 5E, F)."
| PMC
Air Pollutants affects USP49
2 |
Air Pollutants increases the amount of USP49. 2 / 2
2 |
ctd
No evidence text available
32579111
ctd
No evidence text available
34911549
APOBEC3G affects USP49
2 |
USP49 binds APOBEC3G. 2 / 2
2 |
biogrid
No evidence text available
31397674
biogrid
No evidence text available
31397674
6-propyl-2-thiouracil decreases the amount of USP49. 2 / 2
2 |
ctd
No evidence text available
25825206
ctd
No evidence text available
24780913
4,4'-sulfonyldiphenol demethylates USP49.
1 |
4,4'-sulfonyldiphenol demethylates USP49. 1 / 1
1 |
ctd
No evidence text available
33297965
4,4'-sulfonyldiphenol decreases the amount of USP49.
1 |
4,4'-sulfonyldiphenol decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
39298647
Ubiquitinated GRbeta affects USP49
| 1
USP49 binds ubiquitinated GRbeta. 1 / 1
| 1
reach
"USP49 binds ubiquitinated GRbeta and decreases its ubiquitination."
34610959
Tris(1,3-dichloro-2-propyl)phosphate decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
30157643
Triptonide affects USP49
1 |
Triptonide increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
33045310
Trichloroethene affects USP49
1 |
Trichloroethene decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
33387578
Torcetrapib affects USP49
1 |
Torcetrapib increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
23228038
Thiram affects USP49
1 |
Thiram increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
38568856
Thimerosal affects USP49
1 |
Thimerosal decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
27188386
Tetrachloromethane decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
31150632
Temozolomide affects USP49
1 |
Temozolomide decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
31758290
Sunitinib affects USP49
1 |
Sunitinib decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
31533062
Sorafenib affects USP49
1 |
Sorafenib decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
39786591
Silicon dioxide affects USP49
1 |
Silicon dioxide increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
23806026
SgRNA1 affects USP49
| 1
SgRNA1 decreases the amount of USP49. 1 / 1
| 1
reach
"The protein expression of USP49 was reduced by sgRNA1 more significantly than by the other sgRNAs (Fig. 2A, lane 2), which is in line with the high indel percentage observed in sgRNA1 (Supplementary Fig. S1A, B)."
35273362
Schizandrin B affects USP49
1 |
Schizandrin B increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
31150632
Saxitoxin affects USP49
1 |
Saxitoxin decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
39571800
Progesterone affects USP49
1 |
Progesterone decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
19690047
Perfluorooctanesulfonamide decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
39104825
Pentanal affects USP49
1 |
Pentanal decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
26079696
Paracetamol affects USP49
1 |
Paracetamol increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
33387578
Ozone affects USP49
1 |
Ozone increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
34911549
Okadaic acid affects USP49
1 |
Okadaic acid decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
38832940
MiR-5000-3p promoter affects USP49
| 1
USP49 binds miR-5000-3p promoter. 1 / 1
| 1
reach
"Molecular mechanism assays revealed that USP49 binds to the miR-5000-3p promoter to increase the expression of miR-5000-3p, resulting in cancer cells sensitized to OXA."
34075026
MiR-361-3p affects USP49
| 1
MiR-361-3p decreases the amount of USP49. 1 / 1
| 1
reach
"MiR-361-3p could inhibit the expression of Usp49 by binding to its 3' untranslated region."
39568464
Methylmercury chloride decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
28001369
Methyl methanesulfonate increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
23649840
Hydralazine affects USP49
1 |
Hydralazine increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
17183730
Hsa-mir-4284 affects USP49
1 |
Hsa-mir-4284 decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-939-3p affects USP49
1 |
Hsa-miR-939-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-6890-3p affects USP49
1 |
Hsa-miR-6890-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-6852-5p affects USP49
1 |
Hsa-miR-6852-5p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-6836-3p affects USP49
1 |
Hsa-miR-6836-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-6829-3p affects USP49
1 |
Hsa-miR-6829-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-6791-3p affects USP49
1 |
Hsa-miR-6791-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-6783-3p affects USP49
1 |
Hsa-miR-6783-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-6778-3p affects USP49
1 |
Hsa-miR-6778-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-6756-3p affects USP49
1 |
Hsa-miR-6756-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-6742-3p affects USP49
1 |
Hsa-miR-6742-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-6741-3p affects USP49
1 |
Hsa-miR-6741-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-6738-3p affects USP49
1 |
Hsa-miR-6738-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-5088-3p affects USP49
1 |
Hsa-miR-5088-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-5001-3p affects USP49
1 |
Hsa-miR-5001-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-4749-3p affects USP49
1 |
Hsa-miR-4749-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-4727-5p affects USP49
1 |
Hsa-miR-4727-5p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-4638-5p affects USP49
1 |
Hsa-miR-4638-5p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-324-3p affects USP49
1 |
Hsa-miR-324-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-3127-3p affects USP49
1 |
Hsa-miR-3127-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-23b-5p affects USP49
1 |
Hsa-miR-23b-5p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-23a-5p affects USP49
1 |
Hsa-miR-23a-5p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-210-5p affects USP49
1 |
Hsa-miR-210-5p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-1343-3p affects USP49
1 |
Hsa-miR-1343-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-1307-3p affects USP49
1 |
Hsa-miR-1307-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-1237-3p affects USP49
1 |
Hsa-miR-1237-3p decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Formaldehyde affects USP49
1 |
Formaldehyde increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
23649840
Fenvalerate affects USP49
1 |
Fenvalerate increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
30307764
Ethyl methanesulfonate increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
23649840
Entinostat affects USP49
1 |
Entinostat decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
26272509
Endosulfan affects USP49
1 |
Endosulfan increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
29391264
Dorsomorphin affects USP49
1 |
Dorsomorphin decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
27188386
Cycloheximide affects USP49
| 1
Cycloheximide activates USP49. 1 / 1
| 1
reach
"To this end, HCT116 cells stably transfected with or without Flag-USP49 were treated with 50mug/ml cycloheximide (CHX) for various time points, and the results showed a longer half-life of the p53 protein in cells overexpressing USP49."
29748582
Copper atom affects USP49
1 |
Copper atom increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
20971185
Chlorpyrifos affects USP49
1 |
Chlorpyrifos decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
37019170
Centrin affects USP49
| 1
USP49 binds centrin. 1 / 1
| 1
sparser
"For example, the DUB USP49 interacts with the three calcium-binding Centrin proteins that localize to centrosomes and are important for their function, suggesting a connection between DUBs and centros[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19632171
Cadmium atom affects USP49
1 |
Cadmium atom increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
35301059
Bromobenzene affects USP49
1 |
Bromobenzene increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
32479839
Beta-lapachone affects USP49
1 |
Beta-lapachone decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
38218311
Aristolochic acid A increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
33212167
All-trans-retinoic acid decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
21934132
Abrine affects USP49
1 |
Abrine increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
31054353
[6]-gingerol affects USP49
| 1
[6]-gingerol increases the amount of USP49. 1 / 1
| 1
reach
"Interestingly, we also found that [6]-gingerol, an anti-OA drug, could upregulate the protein level of Usp49 and suppress the Wnt and beta-catenin signaling cascade in primary rat chondrocytes."
32737772
ZNF774 affects USP49
1 |
USP49 binds ZNF774. 1 / 1
1 |
biogrid
No evidence text available
32296183
ZIC1 affects USP49
1 |
ZIC1 decreases the amount of USP49. 1 / 1
1 |
biopax:msigdb
No evidence text available
ZHX2 affects USP49
1 |
ZHX2 binds USP49. 1 / 1
1 |
biogrid
No evidence text available
36037364
YARS2 affects USP49
1 |
USP49 binds YARS2. 1 / 1
1 |
biogrid
No evidence text available
19615732
WRNIP1 affects USP49
1 |
USP49 binds WRNIP1. 1 / 1
1 |
biogrid
No evidence text available
35367823
VDR affects USP49
1 |
VDR decreases the amount of USP49. 1 / 1
1 |
biopax:msigdb
No evidence text available
USP9X affects USP49
| 1
USP49 binds USP9X. 1 / 1
| 1
reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
38874156
USP8 affects USP49
| 1
USP49 binds USP8. 1 / 1
| 1
reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
38874156
USP7 affects USP44
| 1
USP25 binds USP44, USP49, USP7, and SOX2. 1 / 1
| 1
sparser
"USP25, USP44, USP49, and USP7 bind to the Sox2 promoter, while USP10, USP16, USP3, USP37, USP44, and USP7 bind to the Nanog promoter."
26880980
USP49 affects ubiquitination proteins
| 1
USP49 inhibits ubiquitination proteins. 1 / 1
| 1
eidos
"Recently , it has been shown that USP49 interacts with p53 , dual-specificity protein phosphatases ( DUSP ) , or FKBP51 in the nucleus , and consequently suppresses the ubiquitination of these proteins ( Luo et al ., 2017 ; Tu et al ., 2018 ; Zhang et al ., 2019 ) ."
31397674
USP49 affects ubiquitinated GRbeta
| 1
USP49 binds ubiquitinated GRbeta. 1 / 1
| 1
reach
"USP49 binds ubiquitinated GRbeta and decreases its ubiquitination."
34610959
USP49 leads to the deubiquitination of tacrolimus (anhydrous). 1 / 1
| 1
reach
"USP49 enhances GEM sensitization by promoting the deubiquitination of FK506-binding protein 51 (FKBP51) and further dephosphorylates AKT (Luo et al., 2017)."
38174069
USP49 affects steady
| 1
USP49 activates steady. 1 / 1
| 1
eidos
"The authors found that a DUB , USP49 , directly interacts with A3G and removes ubiquitins from A3G , increasing its steady state levels and its antiviral activity ."
31397674
USP49 affects several morphological defects
| 1
USP49 inhibits several morphological defects. 1 / 1
| 1
eidos
"USP49 depletion produced several morphological defects during tooth development , such as reduced dentin growth with shrunken enamel space , and abnormal enamel formation including irregular mineralization ."
35273362
USP49 affects rescue virus
| 1
USP49 inhibits rescue virus. 1 / 1
| 1
eidos
"The authors should sequence the proviral DNA in the cultures at the 21-day time point to verify that the rescue in virus was not due to reversion of the Y40H mutation and due to suppression of USP49 ."
31397674
USP49 affects phleomycin
| 1
USP49 inhibits phleomycin. 1 / 1
| 1
reach
"Interestingly, USP49 (CA) over-expression also reduced viability following phleomycin treatment, while it did not affect γH2AX ubiquitylation and not hinder 53BP1 foci formation."
35598681
USP49 affects odontogenesis
| 1
USP49 activates odontogenesis. 1 / 1
| 1
reach
"Depletion of USP49 impairs normal odontogenesis."
35273362
USP49 affects miR-5000-3p promoter
| 1
USP49 binds miR-5000-3p promoter. 1 / 1
| 1
reach
"Molecular mechanism assays revealed that USP49 binds to the miR-5000-3p promoter to increase the expression of miR-5000-3p, resulting in cancer cells sensitized to OXA."
34075026
USP49 affects mgl-1
| 1
USP49 decreases the amount of mgl-1. 1 / 1
| 1
reach
"In contrast, a catalytic mutant USP11 (C266S) and USP49 which were used as negative controls, failed to reduce the ubiquitination level of Mgl-1."
26919101
USP49 affects malonaldehyde
| 1
reach
"Our results indicated that the knockdown of USP49 attenuated the reduction of lipid ROS and MDA induced by HDAC3 overexpression (Fig. 6L, M and Fig. S1G)."
| PMC
USP49 affects inhibitory A3G infectivity HIV-1
| 1
USP49 activates inhibitory A3G infectivity HIV-1. 1 / 1
| 1
eidos
"USP49 enhances the inhibitory effect of A3G on the infectivity of HIV-1 ."
31397674
USP49 affects inhibitory A3G HIV-1 infectivity
| 1
USP49 activates inhibitory A3G HIV-1 infectivity. 1 / 1
| 1
eidos
"USP49 enhances the inhibitory effect of A3G on HIV-1 infectivity To evaluate the inhibitory effect of DUB proteins on the Vif-positive HIV-1 replication , we co-transfected 293 T cells with USP49-specific siRNA , pNL4-3DeltaVif , and various amounts of A3G - and Vif-expressing plasmids ."
31397674
USP49 affects hypermutation
| 1
USP49 activates hypermutation. 1 / 1
| 1
eidos
"This result verifies that the inhibition of USP49 can promote the degradation of A3G and subsequently reduce the hypermutation in virus.These data indicated that the anti-A3G effect of Vif can be enforced by depletion of the A3G protein stabilizer USP49 , alternatively supporting the fact that USP49 enhances the anti-HIV-1 effect of A3G ."
31397674
USP49 affects hypermutation virus
| 1
USP49 activates hypermutation virus. 1 / 1
| 1
eidos
"This result verifies that the inhibition of USP49 can promote the degradation of A3G and subsequently reduce the hypermutation in virus ."
31397674
USP49 affects hippo signaling
| 1
USP49 activates hippo signaling. 1 / 1
| 1
reach
"Two studies have suggested that DUB1 and USP49 also contribute to GC development by regulating the Hippo signaling pathway [58,67]."
36497313
USP49 affects etoposide
| 1
USP49 activates etoposide. 1 / 1
| 1
reach
"Finally, we found that USP49 can increase cell sensitivity to etoposide (Eto)-induced DNA damage and that USP49-knockout mice are more susceptible to colorectal cancer induced by azoxymethane and dextran sulfate sodium (AOM and DSS)."
29748582
USP49 affects endogenous A3G
| 1
USP49 activates endogenous A3G. 1 / 1
| 1
eidos
"USP49 is expected to aid the endogenous A3G to inhibit HIV-1 infectivity ."
31397674
USP49 affects downregulation of A3G
| 1
USP49 inhibits downregulation of A3G. 1 / 1
| 1
eidos
"The results showed that USP49 significantly suppressed the downregulation of A3G when Vif levels were moderate ."
31397674
USP49 affects defective proviruses latently-infected cells
| 1
USP49 activates defective proviruses latently-infected cells. 1 / 1
| 1
eidos
"Combined with the G to A hypermutation measurements , these data suggest that USP49 contributes to the generation of defective proviruses in the HIV-1 latently-infected cells and therefore the actual size of the viral reservoir ."
31397674
USP49 affects colony chemotherapy resistance
| 1
USP49 activates colony chemotherapy resistance. 1 / 1
| 1
eidos
"Knockdown of USP49 markedly inhibited CRC cell proliferation , colony formation , and chemotherapy resistance in vitro ."
35367823
USP49 affects centrin
| 1
USP49 binds centrin. 1 / 1
| 1
sparser
"For example, the DUB USP49 interacts with the three calcium-binding Centrin proteins that localize to centrosomes and are important for their function, suggesting a connection between DUBs and centros[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19632171
USP49 affects cell
| 1
USP49 activates cell. 1 / 1
| 1
eidos
"Knockdown of USP49 markedly inhibited CRC cell proliferation , colony formation , and chemotherapy resistance in vitro ."
35367823
USP49 affects cell death
| 1
USP49 activates cell death. 1 / 1
| 1
eidos
"These findings suggest that USP49 upregulation by DEHP induces cell death via p53 regulation , which subsequently leads to POF due to follicle depletion ."
32143396
USP49 affects cell cycle
| 1
USP49 activates cell cycle. 1 / 1
| 1
reach
"Further flow cytometry analysis showed that overexpressed USP49 induced cell cycle arrest at G0/G1 phase."
31001918
USP49 affects carboplatin
| 1
USP49 activates carboplatin. 1 / 1
| 1
reach
"IGF2BP3-dependent N6-methyladenosine modification of USP49 promotes carboplatin resistance in retinoblastoma by enhancing autophagy via regulating the stabilization of SIRT1 ."
39497328
USP49 affects antiviral agent
| 1
eidos
"The authors found that a DUB , USP49 , directly interacts with A3G and removes ubiquitins from A3G , increasing its steady state levels and its antiviral activity ."
31397674
USP49 affects anti-HIV-1 A3G
| 1
USP49 activates anti-HIV-1 A3G. 1 / 1
| 1
eidos
"This result verifies that the inhibition of USP49 can promote the degradation of A3G and subsequently reduce the hypermutation in virus.These data indicated that the anti-A3G effect of Vif can be enforced by depletion of the A3G protein stabilizer USP49 , alternatively supporting the fact that USP49 enhances the anti-HIV-1 effect of A3G ."
31397674
USP49 affects ZNF774
1 |
USP49 binds ZNF774. 1 / 1
1 |
biogrid
No evidence text available
32296183
USP49 affects ZHX2
1 |
ZHX2 binds USP49. 1 / 1
1 |
biogrid
No evidence text available
36037364
USP49 affects YARS2
1 |
USP49 binds YARS2. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects YAP1
| 1
YAP1 binds USP49. 1 / 1
| 1
sparser
"Our group has previously studied the Hippo-YAP1 pathway and discovered that the USP49-YAP1 feedback loop promotes GC progression [ xref ]."
38493426
USP49 affects Wnt/β-catenin
| 1
USP49 inhibits Wnt/β-catenin. 1 / 1
| 1
reach
"USP49 attenuates OA progression by inhibiting the Wnt/β-catenin pathway."
34216299
USP49 affects Wnt
| 1
USP49 activates Wnt. 1 / 1
| 1
reach
"Taken together, our study not only demonstrates that Usp49 can negatively regulate the progression of OA by inhibiting the Wnt and beta-catenin signaling cascade, but also elucidates the underlying molecular mechanisms."
32737772
USP49 affects WRNIP1
1 |
USP49 binds WRNIP1. 1 / 1
1 |
biogrid
No evidence text available
35367823
USP49 affects Vif
| 1
USP49 inhibits Vif. 1 / 1
| 1
reach
"USP49 inhibits Vif-mediated degradation of A3G."
36574218
USP49 affects Ubiquitination
| 1
eidos
"The authors further demonstrated that USP49 interacted with A3G and reduced its ubiquitination ."
31397674
USP49 affects USP9X
| 1
USP49 binds USP9X. 1 / 1
| 1
reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
38874156
USP49 affects USP8
| 1
USP49 binds USP8. 1 / 1
| 1
reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
38874156
USP49 affects USP33
| 1
USP49 binds USP33. 1 / 1
| 1
reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
38874156
USP49 affects USP25
| 1
USP25 binds USP44, USP49, USP7, and SOX2. 1 / 1
| 1
sparser
"USP25, USP44, USP49, and USP7 bind to the Sox2 promoter, while USP10, USP16, USP3, USP37, USP44, and USP7 bind to the Nanog promoter."
26880980
USP49 affects USP20
| 1
USP49 binds USP20. 1 / 1
| 1
reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
38874156
USP49 affects UBR5
1 |
USP49 binds UBR5. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects U2 snRNPs
| 1
USP49 inhibits U2 snRNPs. 1 / 1
| 1
reach
"Depletion of the H2B deubiquitinase, USP49 caused extensive changes in alternative splicing marked by preferential skipping of exons with elevated H2Bub1 and reduced association of the U1 and U2 snRNPs with chromatin 130."
27107644
USP49 affects TRIM35
1 |
USP49 binds TRIM35. 1 / 1
1 |
biogrid
No evidence text available
23105109
reach
"By stabilizing PTEN, USP49 inhibits non–small cell lung cancer growth (35)."
34610959
USP49 affects STIP1
1 |
USP49 binds STIP1. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects STING aggregation
| 1
USP49 inhibits STING aggregation. 1 / 1
| 1
eidos
"On the contrary , USP49 antagonizes STING activation by removing its K63-linked ubiquitin chains , impedes STING aggregation , and subsequent TBK1 recruitment after HSV-1 invasion ( Ye et al ., 2019 ) ."
34966372
USP49 affects STAMBPL1
1 |
STAMBPL1 binds USP49. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects STAMBP
| 1
USP49 binds STAMBP. 1 / 1
| 1
reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
38874156
USP49 affects SOX2
| 1
USP25 binds USP44, USP49, USP7, and SOX2. 1 / 1
| 1
sparser
"USP25, USP44, USP49, and USP7 bind to the Sox2 promoter, while USP10, USP16, USP3, USP37, USP44, and USP7 bind to the Nanog promoter."
26880980
USP49 affects SNRPB2
| 1
USP49 inhibits SNRPB2. 1 / 1
| 1
reach
"Inhibition of the histone H2B deubiquitinase, USP49, promotes the association of splicing factors U1A and U2B with chromatin and nascent pre-mRNA."
38762032
USP49 affects SNRPA
| 1
USP49 inhibits SNRPA. 1 / 1
| 1
reach
"Inhibition of the histone H2B deubiquitinase, USP49, promotes the association of splicing factors U1A and U2B with chromatin and nascent pre-mRNA."
38762032
USP49 affects SLC39A7
1 |
USP49 binds SLC39A7. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects SHPRH
1 |
USP49 binds SHPRH. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects SHC
| 1
USP49 deubiquitinates SHC. 1 / 1
| 1
reach
"Mechanistically, USP49 deubiquitinates and stabilizes Shc SH2-domain binding protein 1 (SHCBP1), subsequently facilitating the entry of β-catenin into the nucleus to enhance GPX4 transcriptional expression."
39234990
USP49 inhibits Reactive Oxygen Species. 1 / 1
| 1
reach
"Our results indicated that the knockdown of USP49 attenuated the reduction of lipid ROS and MDA induced by HDAC3 overexpression (Fig. 6L, M and Fig. S1G)."
| PMC
RNA, Long Noncoding binds USP49. 1 / 1
| 1
trips
"The long non-coding RNA HLNC1 potentiates hepatocellular carcinoma progression via interaction with USP49."
32691951
USP49 affects RBCK1
1 |
USP49 binds RBCK1. 1 / 1
1 |
biogrid
No evidence text available
23105109
USP49 affects R-induced apoptosis H9c2 cardiomyocytes
| 1
USP49 inhibits R-induced apoptosis H9c2 cardiomyocytes. 1 / 1
| 1
eidos
"35 Some studies have found that ubiquitin-specific peptidase 49 ( USP49 ) and 2,3,5,4 - tetrahydroxystilbene-2-O-beta-d-glucoside ( TSG ) could eliminate the I / R-induced apoptosis of H9c2 cardiomyocytes ,36 while miR-181c-5p exacerbated the hypoxia / reoxygenation ( H / R ) - induced apoptosis of H9c2 cells by targeting PTPN4.37 Nevertheless , no significant progress has been achieved to eliminate I / R injury and MI after decades of investigation ."
33717651
reach
"PTPLAD2 and USP49 Involved in the Pathogenesis of Smoke Induced COPD by Integrative Bioinformatics Analysis."
33116468
USP49 affects Prognosis
| 1
USP49 inhibits Prognosis. 1 / 1
| 1
eidos
"USP49 mediates tumor progression and poor prognosis through a YAP1-dependent feedback loop in gastric cancer ."
35318441
USP49 affects PTCD2
1 |
PTCD2 binds USP49. 1 / 1
1 |
biogrid
No evidence text available
32296183
USP49 affects PRKACA
1 |
USP49 binds PRKACA. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects PPP6R3
1 |
USP49 binds PPP6R3. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects PPP6R2
1 |
USP49 binds PPP6R2. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects PPP6R1
1 |
USP49 binds PPP6R1. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects PPP5C
1 |
USP49 binds PPP5C. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects PPM1G
1 |
USP49 binds PPM1G. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects PHLPP1
| 1
USP49 inhibits PHLPP1. 1 / 1
| 1
reach
"In contrast, another DUB, USP49, inhibits AKT pathway by promoting interaction of PHLPP1 to AKT, thus facilitating the de-phosphorylation of AKT."
28923280
USP49 affects PDCD2
1 |
USP49 binds PDCD2. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects PAX9 MSX1 proteins
| 1
USP49 activates PAX9 MSX1 proteins. 1 / 1
| 1
eidos
"On the other hand , the loss of USP49 reduces the levels of PAX9 and MSX1 proteins , which causes transient retardation of odontogenic differentiation in human dental pulp stem cells and delays the differentiation of human pluripotent stem cells into the neural crest cell lineage ."
35273362
USP49 affects NUP85
1 |
USP49 binds NUP85. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects NFKBIA
| 1
USP49 binds NFKBIA. 1 / 1
| 1
sparser
"Furthermore, we demonstrated that Usp49 binds to IκBα and mediates its deubiquitination, leading to the stabilization of IκBα, which results in the cytoplasmic accumulation of NF-κB and eventually the suppression of NF-κB activity."
39568464
USP49 affects NEK6
| 1
NEK6 binds USP49. 1 / 1
| 1
sparser
"However, we found that NEK6 specifically interacted with USP49 but not NEK3, suggesting that NEK6 was critically involved in USP49 degradation (Supplementary Fig. xref )."
35279684
USP49 affects NAMPT
1 |
NAMPT binds USP49. 1 / 1
1 |
biogrid
No evidence text available
32296183
USP49 affects Mice
| 1
USP49 inhibits Mice. 1 / 1
| 1
reach
"The mock-control and USP49-depleted samples were then transplanted into the subrenal capsules of mice to provide tooth germs with an in vivo biological environment for proper calcification."
35273362
USP49 affects MED8
1 |
USP49 binds MED8. 1 / 1
1 |
hprd
No evidence text available
14576168
USP49 affects MED18
1 |
MED18 binds USP49. 1 / 1
1 |
hprd
No evidence text available
12584197
USP49 affects MED17
1 |
USP49 binds MED17. 1 / 1
1 |
hprd
No evidence text available
14576168
USP49 affects MCM6
1 |
USP49 binds MCM6. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects LRPPRC
1 |
USP49 binds LRPPRC. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects KRR1
1 |
USP49 binds KRR1. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects IVNS1ABP
1 |
USP49 binds IVNS1ABP. 1 / 1
1 |
biogrid
No evidence text available
35367823
USP49 affects IPO9
1 |
USP49 binds IPO9. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects IPO7
1 |
USP49 binds IPO7. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects IMPG1
1 |
USP49 binds IMPG1. 1 / 1
1 |
biogrid
No evidence text available
33961781
USP49 affects IL1B
| 1
USP49 decreases the amount of IL1B. 1 / 1
| 1
reach
"USP49 is lowly expressed in human OA chondrocytes and overexpression of USP49 in primary rat chondrocytes inhibits apoptosis by promoting AXIN deubiquitination and reducing the level of IL-1β, a marker for OA progression (Fig. 1) [26]."
34216299
USP49 affects IL1
| 1
USP49 inhibits IL1. 1 / 1
| 1
reach
"USP49 facilitated the deubiquitination of Axin and suppressed IL-1β-stimulated apoptosis of chondrocytes by inhibition of Wnt/β-catenin signaling [7]."
37814350
USP49 affects Hemagglutinins
| 1
sparser
"We therefore performed a co-immunoprecipitation experiment in which Flag-USP49 and HA-p53 plasmids were separately transfected or co-transfected into 293T cells and potential interactions between Flag-USP49 and HA-p53 were examined (Fig.  xref )."
29748582
USP49 affects HUWE1
1 |
USP49 binds HUWE1. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects HSPH1
1 |
USP49 binds HSPH1. 1 / 1
1 |
biogrid
No evidence text available
35367823
USP49 affects HIV-1 replication
| 1
USP49 inhibits HIV-1 replication. 1 / 1
| 1
eidos
"Thank you for submitting your article `` USP49 potently stabilizes APOBEC3G protein and inhibits HIV-1 replication '' for consideration by eLife ."
31397674
USP49 affects HIV infectivity
| 1
USP49 inhibits HIV infectivity. 1 / 1
| 1
eidos
"It is important to show that USP49 works with the endogenous A3G to inhibit HIV infectivity ."
31397674
USP49 affects HADHA
1 |
USP49 binds HADHA. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects H3K18
| 1
USP49 activates H3K18. 1 / 1
| 1
reach
"Mechanistically, CAP facilitates the ubiquitination of the HDAC3 protein through the down-regulation of USP49, which in turn up-regulates the lactylation of H3K18."
| PMC
USP49 affects H2Bub
| 1
USP49 inhibits H2Bub. 1 / 1
| 1
reach
"USP49 knockout increased the H2Bub enrichment at MDM2 gene whereas USP49 overexpression downregulated the H2Bub level at MDM2 gene."
35072515
USP49 affects H2BLys120
| 1
USP49 deubiquitinates H2BLys120. 1 / 1
| 1
reach
"On the other hand, USP49 was also known to deubiquitylate H2BLys120, which promoted both HR and NHEJ, ( Moyal 2011, Nakamura, 2011; So et al., 2019 ), suggesting that ZnF-UBP domain of USP49 might fun[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35598681
USP49 affects H2BC10
1 |
USP49 deubiquitinates H2BC10. 1 / 1
1 |
ubibrowser
"<span class="match term0">USP49</span> deubiquitinates histone <span class="match term1">H2B</span> and regulates cotranscriptional pre-mRNA splicing"
23824326
USP49 affects GTF2H1
| 1
USP49 decreases the amount of GTF2H1. 1 / 1
| 1
reach
"Western blotting results revealed that USP49 overexpression significantly increased Beclin1 expression and reduced P62 expression in Y‐79 cells, while USP49 deletion exerted opposite effects on Beclin1 and P62 expression in Y‐79/CBP cells (Figure 4A)."
39497328
USP49 affects GST
| 1
TP53 binds GST, USP49, and Flag. 1 / 1
| 1
sparser
"This interaction was further confirmed in a GST pull-down assay, with GST-p53 specifically interacting with transfected Flag-USP49, as shown in Fig.  xref ."
29748582
USP49 affects GRbeta
| 1
USP49 activates GRbeta. 1 / 1
| 1
reach
"USP49 contributes oncogenic functions of GRbeta in GBM."
34610959
USP49 affects FKBP51-AKT
| 1
USP49 activates FKBP51-AKT. 1 / 1
| 1
reach
"USP49 is also reported to suppress tumorigenesis and chemo-responses in pancreatic cancer by targeting FKBP51-AKT signaling 29."
29748582
USP49 affects F7
1 |
USP49 binds F7. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects Death
| 1
USP49 inhibits Death. 1 / 1
| 1
reach
"Consistently, overexpression of USP49 reduced chondrocyte apoptotic death that was induced by IL-1β stimulation via enhancement of Axin deubiquitination, leading to upregulation of Axin protein levels."
37359559
USP49 affects DKK1
| 1
USP49 activates DKK1. 1 / 1
| 1
reach
"Zc3h11a, Rnasek, Usp49, Btbd6, and Ly6c1 were the 5 most highly upregulated genes upon treatment with DKK1 (Supplemental Figure 8D)."
34847079
USP49 affects DICER1
1 |
USP49 binds DICER1. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects DDR
| 1
USP49 activates DDR. 1 / 1
| 1
reach
"Furthermore, we previously identified USP49 as a potential DDR factor in a screen, although how USP49 contributes to DDR was not investigated ( Nishi et al., 2014 )."
35598681
reach
"USP49 was also reported to inhibit non-small cell lung cancer cell growth by suppressing PI3K/AKT signaling ."
34075026
USP49 affects Carcinogenesis
| 1
USP49 inhibits Carcinogenesis. 1 / 1
| 1
reach
"A previous study suggested depletion of USP49 increased pancreatic cancer cell proliferation in vitro, tumorigenesis in vivo, and chemoresistance, proposing that USP49 regulates tumorigenesis and chemoresistance in pancreatic cancer in an AKT-dependent manner ."
34075026
USP49 affects COPA
1 |
USP49 binds COPA. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects Bcl‐2‐Associated Athanogene 2
| 1
USP49 deubiquitinates Bcl‐2‐Associated Athanogene 2. 1 / 1
| 1
reach
"20 For instance, USP49 deubiquitinates and stabilizes Bcl‐2‐Associated Athanogene 2 (BAG2) which is a well‐known oncogenic protein that antagonizes apoptosis and enables adaptive response in colorectal cancer."
39497328
USP49 affects BECN1
| 1
USP49 increases the amount of BECN1. 1 / 1
| 1
reach
"Western blotting results revealed that USP49 overexpression significantly increased Beclin1 expression and reduced P62 expression in Y‐79 cells, while USP49 deletion exerted opposite effects on Beclin1 and P62 expression in Y‐79/CBP cells (Figure 4A)."
39497328
USP49 affects Adenocarcinoma
| 1
USP49 activates Adenocarcinoma. 1 / 1
| 1
reach
"USP49 promotes adenocarcinoma of the esophagogastric junction malignant progression via activating SHCBP1-β-catenin-GPX4 axis."
39234990
USP49 affects AXIN1
| 1
USP49 leads to the deubiquitination of AXIN1. 1 / 1
| 1
reach
"Reports have indicated that deubiquitinating enzymes, including USP7 [69], USP44 [70], USP49 [71], and UCHL5 [72], can reduce the ubiquitination of AXIN1, thereby hampering WNT/β-catenin signaling."
38291457
USP49 affects ANKRD52
1 |
USP49 binds ANKRD52. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects ANKRD28
1 |
USP49 binds ANKRD28. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP49 affects 8
| 1
USP49 activates 8. 1 / 1
| 1
reach
"USP49 directly increases the stability of FKBP51, a scaffolding protein in the interaction between AKT and PHLPP, and thus enhances sensitivity to gemcitabine."
35760282
USP49 affects 2 C
| 1
USP49 binds H2AX and 2 C. 1 / 1
| 1
reach
"The colocalization of USP49 (CA) and γH2AX prompted us to investigate the protein–protein interaction between USP49 (CA) and γH2AX ( Fig. 2 C)."
35598681
USP44 affects USP7
| 1
USP25 binds USP44, USP49, USP7, and SOX2. 1 / 1
| 1
sparser
"USP25, USP44, USP49, and USP7 bind to the Sox2 promoter, while USP10, USP16, USP3, USP37, USP44, and USP7 bind to the Nanog promoter."
26880980
USP33 affects USP49
| 1
USP49 binds USP33. 1 / 1
| 1
reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
38874156
USP25 affects USP7
| 1
USP25 binds USP44, USP49, USP7, and SOX2. 1 / 1
| 1
sparser
"USP25, USP44, USP49, and USP7 bind to the Sox2 promoter, while USP10, USP16, USP3, USP37, USP44, and USP7 bind to the Nanog promoter."
26880980
USP20 affects USP49
| 1
USP49 binds USP20. 1 / 1
| 1
reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
38874156
UBR5 affects USP49
1 |
USP49 binds UBR5. 1 / 1
1 |
biogrid
No evidence text available
19615732
Treating primary rat chondrocytes different IL-1beta affects USP49
| 1
Treating primary rat chondrocytes different IL-1beta inhibits USP49. 1 / 1
| 1
eidos
"Treating primary rat chondrocytes with different concentrations of IL-1beta resulted in decreased Usp49 expression , while Usp49 overexpression could attenuate IL-1beta-induced chondrocyte apoptosis by promoting Axin deubiquitination ."
32737772
Tobacco Smoke Pollution decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
33660061
TRIM35 affects USP49
1 |
USP49 binds TRIM35. 1 / 1
1 |
biogrid
No evidence text available
23105109
TP53 affects Hemagglutinins
| 1
sparser
"We therefore performed a co-immunoprecipitation experiment in which Flag-USP49 and HA-p53 plasmids were separately transfected or co-transfected into 293T cells and potential interactions between Flag-USP49 and HA-p53 were examined (Fig.  xref )."
29748582
TP53 affects GST
| 1
TP53 binds GST, USP49, and Flag. 1 / 1
| 1
sparser
"This interaction was further confirmed in a GST pull-down assay, with GST-p53 specifically interacting with transfected Flag-USP49, as shown in Fig.  xref ."
29748582
TNF affects USP49
| 1
TNF activates USP49. 1 / 1
| 1
reach
"Nonetheless, a minimum of two siRNA molecules led to increased levels of nuclear NF-kappaB in A549 cells depleted of A20, USP7, USP49, USP54, or FBXO36 and treated with TNF for 120min."
25071782
TFAP2C affects USP49
1 |
TFAP2C decreases the amount of USP49. 1 / 1
1 |
biopax:msigdb
No evidence text available
TFAP2A affects USP49
1 |
TFAP2A decreases the amount of USP49. 1 / 1
1 |
biopax:msigdb
No evidence text available
Soot affects USP49
1 |
Soot increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
34911549
Soman affects USP49
1 |
Soman increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
19281266
STIP1 affects USP49
1 |
USP49 binds STIP1. 1 / 1
1 |
biogrid
No evidence text available
19615732
STAMBPL1 affects USP49
1 |
STAMBPL1 binds USP49. 1 / 1
1 |
biogrid
No evidence text available
19615732
STAMBP affects USP49
| 1
USP49 binds STAMBP. 1 / 1
| 1
reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
38874156
SOX2 affects USP7
| 1
USP25 binds USP44, USP49, USP7, and SOX2. 1 / 1
| 1
sparser
"USP25, USP44, USP49, and USP7 bind to the Sox2 promoter, while USP10, USP16, USP3, USP37, USP44, and USP7 bind to the Nanog promoter."
26880980
SLC39A7 affects USP49
1 |
USP49 binds SLC39A7. 1 / 1
1 |
biogrid
No evidence text available
19615732
SHPRH affects USP49
1 |
USP49 binds SHPRH. 1 / 1
1 |
biogrid
No evidence text available
19615732
SHCBP1 affects USP49
1 |
USP49 binds SHCBP1. 1 / 1
1 |
biogrid
No evidence text available
19615732
RUVBL1 affects PSMC5
| 1
USP49 binds RUVBL1 and PSMC5. 1 / 1
| 1
sparser
"USP49 forms a complex with RuvB-like1 (RVB1) and SUG1 and specifically deubiquitinates histone H2B in vitro and in vivo."
23824326
RNAi affects USP49
| 1
RNAi activates USP49. 1 / 1
| 1
reach
"qPCR data suggest that RNAi mediated inactivation of USP49, FBXO36, and USP12 depleted levels of the corresponding transcripts."
25071782
RNA, Long Noncoding binds USP49. 1 / 1
| 1
trips
"The long non-coding RNA HLNC1 potentiates hepatocellular carcinoma progression via interaction with USP49."
32691951
REPIN1 affects USP49
1 |
REPIN1 decreases the amount of USP49. 1 / 1
1 |
biopax:msigdb
No evidence text available
RBCK1 affects USP49
1 |
USP49 binds RBCK1. 1 / 1
1 |
biogrid
No evidence text available
23105109
RB1 affects USP49
| 1
RB1 increases the amount of USP49. 1 / 1
| 1
reach
"Altogether, SIRT1 is required for USP49‐mediated malignant proliferation and CBP resistance in RB in vitro.3.7 IGF2BP3 upregulates USP49 expression in an m 6 A-dependent manner."
39497328
Particulate Matter increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
32579111
PTCD2 affects USP49
1 |
PTCD2 binds USP49. 1 / 1
1 |
biogrid
No evidence text available
32296183
PSMC5 affects RUVBL1, and USP49
| 1
USP49 binds RUVBL1 and PSMC5. 1 / 1
| 1
sparser
"USP49 forms a complex with RuvB-like1 (RVB1) and SUG1 and specifically deubiquitinates histone H2B in vitro and in vivo."
23824326
PRKACA affects USP49
1 |
USP49 binds PRKACA. 1 / 1
1 |
biogrid
No evidence text available
19615732
PPP6R3 affects USP49
1 |
USP49 binds PPP6R3. 1 / 1
1 |
biogrid
No evidence text available
19615732
PPP6R2 affects USP49
1 |
USP49 binds PPP6R2. 1 / 1
1 |
biogrid
No evidence text available
19615732
PPP6R1 affects USP49
1 |
USP49 binds PPP6R1. 1 / 1
1 |
biogrid
No evidence text available
19615732
PPP5C affects USP49
1 |
USP49 binds PPP5C. 1 / 1
1 |
biogrid
No evidence text available
19615732
PPM1G affects USP49
1 |
USP49 binds PPM1G. 1 / 1
1 |
biogrid
No evidence text available
19615732
PDCD2 affects USP49
1 |
USP49 binds PDCD2. 1 / 1
1 |
biogrid
No evidence text available
19615732
PCB138 affects USP49
1 |
PCB138 increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
25510870
PCB 180 affects USP49
1 |
PCB 180 increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
25510870
NUP85 affects USP49
1 |
USP49 binds NUP85. 1 / 1
1 |
biogrid
No evidence text available
19615732
NSC 689534 affects USP49
1 |
NSC 689534 increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
20971185
NFKBIA affects USP49
| 1
USP49 binds NFKBIA. 1 / 1
| 1
sparser
"Furthermore, we demonstrated that Usp49 binds to IκBα and mediates its deubiquitination, leading to the stabilization of IκBα, which results in the cytoplasmic accumulation of NF-κB and eventually the suppression of NF-κB activity."
39568464
NAMPT affects USP49
1 |
NAMPT binds USP49. 1 / 1
1 |
biogrid
No evidence text available
32296183
N-methyl-4-phenylpyridinium increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
28801915
MYOD1 affects USP49
1 |
MYOD1 decreases the amount of USP49. 1 / 1
1 |
biopax:msigdb
No evidence text available
MTH-68/H affects USP49
| 1
MTH-68/H activates USP49. 1 / 1
| 1
reach
"Usp18 , Usp49 ) were induced by MTH-68/H infection."
24637408
MIR1913 affects USP49
1 |
MIR1913 decreases the amount of USP49. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
MED8 affects USP49
1 |
USP49 binds MED8. 1 / 1
1 |
hprd
No evidence text available
14576168
MED18 affects USP49
1 |
MED18 binds USP49. 1 / 1
1 |
hprd
No evidence text available
12584197
MED17 affects USP49
1 |
USP49 binds MED17. 1 / 1
1 |
hprd
No evidence text available
14576168
MDM2 affects USP49
| 1
USP49 binds MDM2. 1 / 1
| 1
sparser
"Moreover, chromatin immunoprecipitation revealed that USP49 directly bound to the promoter of MDM2 gene."
35072515
MCM6 affects USP49
1 |
USP49 binds MCM6. 1 / 1
1 |
biogrid
No evidence text available
19615732
LRPPRC affects USP49
1 |
USP49 binds LRPPRC. 1 / 1
1 |
biogrid
No evidence text available
19615732
KRR1 affects USP49
1 |
USP49 binds KRR1. 1 / 1
1 |
biogrid
No evidence text available
19615732
JNK affects USP49
| 1
JNK activates USP49. 1 / 1
| 1
sparser
"Moreover, it has been reported that USP49 overexpression could inhibit JNK1/2 activation and reduce human cardiomyocyte apoptosis, while JNK1/2 inhibitor treatment (SP600125) suppressed USP49 knockdown-induced JNK1/2 activation and cardiomyocyte apoptosis [ xref ]."
38902590
Infections affects USP49
| 1
Infections activates USP49. 1 / 1
| 1
reach
"Usp18 , Usp49 ) were induced by MTH-68/H infection."
24637408
IVNS1ABP affects USP49
1 |
USP49 binds IVNS1ABP. 1 / 1
1 |
biogrid
No evidence text available
35367823
IPO9 affects USP49
1 |
USP49 binds IPO9. 1 / 1
1 |
biogrid
No evidence text available
19615732
IPO7 affects USP49
1 |
USP49 binds IPO7. 1 / 1
1 |
biogrid
No evidence text available
19615732
INS affects USP49
| 1
INS activates USP49. 1 / 1
| 1
reach
"In addition, we explored including the N6‐methyladenosine (m A) modification of USP49 mediated by insulin‐like growth factor 2 mRNA‐binding protein 3 (IGF2BP3) and the impact of USP49 on the stabilization of sirtuin 1 (SIRT1)."
39497328
IMPG1 affects USP49
1 |
USP49 binds IMPG1. 1 / 1
1 |
biogrid
No evidence text available
33961781
Histone_H2B affects USP49
| 1
Histone_H2B activates USP49. 1 / 1
| 1
reach
"Correction for Shi et al., "USP49-Mediated Histone H2B Deubiquitination Regulates HCT116 Cell Proliferation through MDM2-p53 Axis"."
35546121
Hemagglutinins affects USP49
| 1
sparser
"We therefore performed a co-immunoprecipitation experiment in which Flag-USP49 and HA-p53 plasmids were separately transfected or co-transfected into 293T cells and potential interactions between Flag-USP49 and HA-p53 were examined (Fig.  xref )."
29748582
HUWE1 affects USP49
1 |
USP49 binds HUWE1. 1 / 1
1 |
biogrid
No evidence text available
19615732
HSPH1 affects USP49
1 |
USP49 binds HSPH1. 1 / 1
1 |
biogrid
No evidence text available
35367823
HAND1 affects USP49
1 |
HAND1 decreases the amount of USP49. 1 / 1
1 |
biopax:msigdb
No evidence text available
HADHA affects USP49
1 |
USP49 binds HADHA. 1 / 1
1 |
biogrid
No evidence text available
19615732
H2BC21 affects USP49
1 |
USP49 binds H2BC21. 1 / 1
1 |
biogrid
No evidence text available
23824326
H2AX affects 2 C
| 1
USP49 binds H2AX and 2 C. 1 / 1
| 1
reach
"The colocalization of USP49 (CA) and γH2AX prompted us to investigate the protein–protein interaction between USP49 (CA) and γH2AX ( Fig. 2 C)."
35598681
Gentamicins affects USP49
1 |
Gentamicins increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
33387578
GST affects USP49
| 1
TP53 binds GST, USP49, and Flag. 1 / 1
| 1
sparser
"This interaction was further confirmed in a GST pull-down assay, with GST-p53 specifically interacting with transfected Flag-USP49, as shown in Fig.  xref ."
29748582
Flag affects Hemagglutinins, TP53, and USP49
| 1
sparser
"We therefore performed a co-immunoprecipitation experiment in which Flag-USP49 and HA-p53 plasmids were separately transfected or co-transfected into 293T cells and potential interactions between Flag-USP49 and HA-p53 were examined (Fig.  xref )."
29748582
Flag affects GST, TP53, and USP49
| 1
TP53 binds GST, USP49, and Flag. 1 / 1
| 1
sparser
"This interaction was further confirmed in a GST pull-down assay, with GST-p53 specifically interacting with transfected Flag-USP49, as shown in Fig.  xref ."
29748582
FOXO4 affects USP49
1 |
FOXO4 decreases the amount of USP49. 1 / 1
1 |
biopax:msigdb
No evidence text available
FBXO28 affects USP49
| 1
FBXO28 ubiquitinates USP49. 1 / 1
| 1
reach
"FBXO28 contributes to the proliferation of PDAC by promoting ubiquitin-mediated degradation of SMARCC2 (Liu et al., 2023a), and FBXO45 facilitates cell proliferation by ubiquitinating USP49 through binding to its SPRY domain (Wu et al., 2022a)."
38174069
F7 affects USP49
1 |
USP49 binds F7. 1 / 1
1 |
biogrid
No evidence text available
19615732
Dietary Fats affects USP49
1 |
Dietary Fats increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
25016146
DICER1 affects USP49
1 |
USP49 binds DICER1. 1 / 1
1 |
biogrid
No evidence text available
19615732
CTNNB1 affects USP49
1 |
USP49 binds CTNNB1. 1 / 1
1 |
biogrid
No evidence text available
30323974
COPA affects USP49
1 |
USP49 binds COPA. 1 / 1
1 |
biogrid
No evidence text available
19615732
ctd
No evidence text available
26680231
AXIN affects USP49
| 1
USP49 binds AXIN. 1 / 1
| 1
sparser
"Mechanistically, USP49 interacts with Axin for deubiquitination, which downregulates Wnt/β-catenin signaling to mitigate chondrocyte apoptosis [69] ."
38583240
ANKRD52 affects USP49
1 |
USP49 binds ANKRD52. 1 / 1
1 |
biogrid
No evidence text available
19615732
ANKRD28 affects USP49
1 |
USP49 binds ANKRD28. 1 / 1
1 |
biogrid
No evidence text available
19615732
6-gingerol affects USP49
| 1
6-gingerol increases the amount of USP49. 1 / 1
| 1
reach
"In addition, the study of Yang et al. found that 6-gingerol can significantly up-regulate the level of USP49 in OA chondrocytes, and the expression of the latter is significantly reduced in OA patients, resulting in the failure of Axin protein deubiquitination and excessive accumulation leading to apoptosis of chondrocytes [121]."
40226783
6-Gingerol affects USP49
| 1
6-Gingerol increases the amount of USP49. 1 / 1
| 1
reach
"6-Gingerol (17) upregulates the expression of ubiquitin-specific peptidase 49 (USP49), promotes the deubiquitination of Axin, and increases the degradation of β-catenin, resulting in the inhibition of Wnt/β-catenin signaling and the attenuation of apoptosis in IL-1β-treated rat chondrocytes (Yang L. et al., 2020)."
37324450
2-hydroxypropanoic acid increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
30851411
2 C affects USP49
| 1
USP49 binds H2AX and 2 C. 1 / 1
| 1
reach
"The colocalization of USP49 (CA) and γH2AX prompted us to investigate the protein–protein interaction between USP49 (CA) and γH2AX ( Fig. 2 C)."
35598681
ctd
No evidence text available
39104825
17beta-estradiol affects USP49
1 |
17beta-estradiol increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
32622090
1,2-dimethylhydrazine decreases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
22206623
2,4,5,2',5'-pentachlorobiphenyl increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
25510870
2,4,4'-trichlorobiphenyl increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
25510870
2,2',5,5'-tetrachlorobiphenyl increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
25510870
2,2',4,4',5,5'-hexachlorobiphenyl increases the amount of USP49. 1 / 1
1 |
ctd
No evidence text available
25510870