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BTRC binds USP47. 10 / 33

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No evidence text available

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"The interaction of USP47 and β-TrCP is regulated by ERAP1 xref , suggesting that each has a fine-tuned regulatory mechanism."

37740002

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"However, USP47 can both interact with and deubiquitinate β-TrCP."

37740002

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"The interaction of USP47 and β-TrCP is regulated by ERAP1 , suggesting that each has a fine-tuned regulatory mechanism."

37740002

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biogrid

No evidence text available

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"Interaction between USP47 and β-TrCP, a subunit of the SCF β-TrCP E3 ubiquitin ligase complex, is involved in a number of signaling pathways [164,165,166]."

36497313

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No evidence text available

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"Moreover, Peschiaroli et al. revealed that BTRC specifically binds to USP47 to regulate cell survival [40]."

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BTRC binds USP47. 10 / 33

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No evidence text available

25900982

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"The interaction of USP47 and β-TrCP is regulated by ERAP1 xref , suggesting that each has a fine-tuned regulatory mechanism."

37740002

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"However, USP47 can both interact with and deubiquitinate β-TrCP."

37740002

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"The interaction of USP47 and β-TrCP is regulated by ERAP1 , suggesting that each has a fine-tuned regulatory mechanism."

37740002

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biogrid

No evidence text available

28514442

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"Interaction between USP47 and β-TrCP, a subunit of the SCF β-TrCP E3 ubiquitin ligase complex, is involved in a number of signaling pathways [164,165,166]."

36497313

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No evidence text available

19966869

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"Moreover, Peschiaroli et al. revealed that BTRC specifically binds to USP47 to regulate cell survival [40]."

35509185

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34604226

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No evidence text available

25900982

BTRC ubiquitinates USP47.

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BTRC leads to the ubiquitination of USP47. 3 / 3

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"Furthermore, USP47 deubiquitinates itself, whereas β-TrCP promotes USP47 ubiquitination through interaction with an atypical motif in USP47."

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"Furthermore, USP47 deubiquitinates itself, whereas beta-TrCP promotes USP47 ubiquitination through interaction with an atypical motif in USP47."

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"UbiNet 2.0"

24658274

USP47 affects SNAI1

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USP47 binds SNAI1.

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USP47 binds SNAI1. 10 / 18

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"These results underscore the role of USP47 in promoting EMT signaling and suggest that the rs72856331 G allele enhances this effect, contributing to the aggressive behavior of GC cells.Given the known interaction between USP47 and Snai1, a key modulator of EMT (34, 35), we hypothesized that USP47 might induce EMT activation by regulating deubiquitination and protein stability of Snai1 in GC."

39998882

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"In a normal state, USP47 binds to E-cadherin, preventing its degradation; however, when cells are under hypoxic conditions or during EMT, the binding of USP47 to Snail and SATB1 is enhanced, thereby inhibiting the expression of E-cadherin."

34604226

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"Co-immunoprecipitation (Co-IP) assays using a USP47 antibody confirmed a physical interaction between USP47 and Snai1 ( xref )."

39998882

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"Taken together, these results indicate that USP47 interacts directly with Snail and impedes its ubiquitination and subsequent proteasomal degradation."

29162839

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"USP47 binds to Snail and triggers its deubiquitination."

29162839

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"However, under hypoxic conditions, USP47 binding to Snail was markedly enhanced (Fig. xref )."

29162839

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"To visualize the interaction between Snail and USP47, we stained the DLD-1 cells with fluorescent probes detecting USP47 (red) and Snail (green)."

29162839

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"Notably, USP47 interacts directly with Snail and enhances its stability."

29162839

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"USP47 binds to Snail and triggers its deubiquitination."

29162839

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"However, under hypoxic conditions, USP47 binding to Snail was markedly enhanced."

29162839

SNAI1 binds SATB1 and USP47. 1 / 1

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34604226

USP47 activates SNAI1.

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USP47 activates SNAI1. 6 / 6

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"Interestingly, treatment with a pharmacological inhibitor targeting USP47 (P5091) upregulated E-cadherin and downregulated SNAIL both in the nucleus and cytoplasm, while reducing morphological changes otherwise observed in MCF10A cells treated with TGF-β2 [78]."

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"Consistently, the depletion of USP47 by shRNAs enhanced Snai1 turnover in both HGC-27 and GT38 cells (Supplemental Figure 12G)."

39998882

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"Additionally, chemical inhibition of USP47 reduced the expressions of several EMT markers, including CDH1, CTNNB1, and SNAIL, and reversed the morphological changes in MCF-10A cells undergoing EMT (Silvestrini et al., 2020)."

34604226

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"Silencing of USP47 accelerated the proteasomal degradation of Snail and inhibited EMT."

29162839

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"Additionally, in the presence of protein translation inhibitor cycloheximide (CHX), the degradation of USP47 markedly accelerated the protein turnover of Snai1 in HGC-27 cells (Supplemental Figure 12F)."

39998882

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"Because hypoxia induced USP47 expression enhanced EMT by stabilizing Snail, we investigated the contribution of USP47 to tumor growth and invasion in vivo."

29162839

USP47 increases the amount of SNAI1.

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USP47 increases the amount of SNAI1. 5 / 5

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"Next, to determine whether USP47 promotes GC development through maintaining Snai1 levels, we induced the ectopic expression of Snai1 in USP47-depleted cells (Figure 6G and Supplemental Figure 14A)."

39998882

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"We found that the USP47 elevated the protein level of Snail without influencing the expression of its mRNA."

29162839

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"The inhibition of USP47 in combination with TGFβ2 treatment, decreases levels of SNAIL in the nucleus and cytoplasm as demonstrated by both high content screening and western blotting analysis."

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"In HGC-27 cells, USP47 knockdown reduced Snai1 protein levels without affecting its mRNA expression (Figure 6D), indicating a posttranslational regulation of Snai1 by USP47."

39998882

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"Transient knockdown of USP47 in DLD-1 cells lowered the expression of Snail protein."

29162839

USP47 deubiquitinates SNAI1.

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USP47 deubiquitinates SNAI1. 3 / 3

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"USP47, which deubiquitinates SNAIL1, is upregulated in hypoxia via a transcription factor cascade."

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"USP47 has been shown to induce the decomposition of E-cadherin and promote the epithelial–mesenchymal transition via Snail-deubiquitination (Choi et al., 2017)."

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"Hypoxia induces epithelial-mesenchymal transition in colorectal cancer cells through ubiquitin-specific protease 47-mediated stabilization of Snail: A potential role of Sox9"

29162839

USP47 affects cell population proliferation

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USP47 activates cell population proliferation.

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USP47 activates cell population proliferation. 10 / 27

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"Ubiquitin specific peptidase 47 promotes proliferation of lung squamous cell carcinoma."

35254655

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"USP47 functions as a DUB for YAP in colorectal cancer, USP47 elevation leads to the stabilization of YAP and promotes colorectal cancer cell proliferation [11]."

37349820

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"Functional analysis shows that USP47 knockdown represses proliferation of CML cells sensitive or resistant to imatinib in vitro and in vivo."

33397955

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"Specifically, we demonstrate that USP47 is highly expressed in primary CML cells and promotes cell proliferation, while Usp47 knockout significantly prolongs the survival of BCR-ABL and BCR-ABL T315I -induced CML mice by reducing leukemia stem and progenitor cells."

33397955

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"USP47 knockdown inhibits proliferation of CML cells."

33397955

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"Knockdown of USP47 expression in PTC cell lines (TPC-1 and K1), decreased the cell proliferation mobility and invasion capacities, whereas USP47 overexpression in these cell lines showed an inverse effect and promoted cell glycolysis and glutamine metabolism."

37564825

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"Silencing Usp47 inhibits BCR-ABL T315I -induced CML and proliferation of KBM T315I in a xenograft mouse model."

33397955

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"USP47 deficiency impairs transcriptional activity of SATB1 target genes and inhibits proliferation, migration, and tumorigenesis (68, 69)."

40071088

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"We further show that USP47 deficiency represses cell proliferation and migration through SATB1."

30742943

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"USP47 functions as a DUB for YAP in colorectal cancer, USP47 elevation leads to stabilization of YAP and promotes colorectal cancer cell proliferation XREF_BIBR."

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USP47 inhibits cell population proliferation.

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USP47 inhibits cell population proliferation. 5 / 5

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"Downregulation of USP47 inhibited the proliferation of imatinib-sensitive or drug-resistant CML cells."

34604226

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"Here, we demonstrate that miR-101-3p regulates the RPL11-MDM2-p53 pathway by targeting ubiquitin-specific peptidase 47 (USP47), consequently inhibiting cancer cell proliferation."

35205710

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"Moreover, USP47 knockdown could repress CRC cell proliferation, which could be rescued by YAP overexpression (Pan et al., 2020)."

34604226

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"The deficiency of USP47 impaired the transcriptional activity of SATB1 target genes and inhibited cell proliferation, migration, and tumorigenesis in a mouse model of colon cancer."

34604226

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"Of note, USP47 KO did not affect cell proliferation, while USP7/USP47 DKO led to reduced cell proliferation (Supplemental Fig. S3A,B)."

36302555

USP47 affects SATB1

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USP47 binds SATB1.

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SATB1 binds USP47. 10 / 13

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"Together with our findings that SATB1 interacted with USP47, these results indicate that deubiquitinase activity is required for USP47 to remove ubiquitin from SATB1."

30742943

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"SATB1 expression promoted tumor cell glycolysis and glutamine metabolism, while USP47 protein bound to and deubiquitinated SATB1 to increase its intracellular levels, thus promoting glycolysis and glutamine metabolism."

37564825

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"To explore the roles of USP47-SATB1 interactions in CRC metastasis, we examined cell migration and invasion."

30742943

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"As seen in Fig. 2 E, this mutant failed to inhibit the ubiquitination of SATB1 protein without affecting USP47 interaction with SATB1 ( Fig. 2 Eand F)."

30742943

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No evidence text available

30742943

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"USP47 interacted with SATB1 through its catalytic core domain and antagonized Smurf2-mediated SATB1 degradation."

34604226

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No evidence text available

30742943

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No evidence text available

30742943

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"Here, we demonstrate that USP47 interacts with SATB1 to mediate its ubiquitination and positively regulate its stability."

30742943

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"To further confirm that the interaction between SATB1 and USP47 is specific, we transfected these alone or together into 293T cells and evaluated their interaction by immunoprecipitation and western b[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

30742943

SNAI1 binds SATB1 and USP47. 1 / 1

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USP47 activates SATB1.

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USP47 activates SATB1. 8 / 8

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"The deficiency of USP47 impaired the transcriptional activity of SATB1 target genes and inhibited cell proliferation, migration, and tumorigenesis in a mouse model of colon cancer."

34604226

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"These results suggested that USP47 could mediate EMT by stabilizing SATB1 (Yu et al., 2019)."

34604226

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"USP47 deficiency impairs transcriptional activity of SATB1 target genes and inhibits proliferation, migration, and tumorigenesis (68, 69)."

40071088

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"As shown in Fig. 2 G and H, and S3B , the catalytically inactive USP47/CS mutant failed to protect SATB1 from degradation, implying that deubiquitinase activity is required for USP47-mediated SATB1 st[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

30742943

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"Likewise, knockdown of USP47 in HCT116 cells promoted SATB1 degradation ( Fig. S3C )."

30742943

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"In addition, we determined whether USP47-mediated SATB1 stability is achieved via proteasomes."

30742943

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"As seen from Fig. 2 I, MG132 (proteasome inhibitor) treatment could rescue the USP47 knockout-mediated degradation of the SATB1 protein."

30742943

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"We also provided evidence that USP47 positively regulated SATB1 stability."

30742943

USP47 ubiquitinates SATB1.

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USP47 ubiquitinates SATB1. 4 / 4

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"Additionally, reversible ubiquitination of SATB1 by USP47 and SMURF2 promotes colon cancer proliferation ( xref )."

41208974

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"USP47 and SMURF2 co-operates together to mediate the reversible regulation of SATB1 ubiquitination, thereby influencing colon cancer cell proliferation and tumor progression [ 26 ]."

37422058

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"For example, modulation of SATB1 ubiquitination by USP47 and SMURF2 affects proliferative ability of colon cancer cells as well as the tumor progression [ 21 ]."

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"For example, modulation of SATB1 ubiquitination by USP47 and SMURF2 affects proliferative ability of colon cancer cells as well as the tumor progression [ 21 ]."

37597669

USP47 deubiquitinates SATB1.

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USP47 deubiquitinates SATB1. 2 / 2

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"Also, SATB1 , DNA polymerase β , etc., are deubiquitinated only by USP47."

37740002

USP47 decreases the amount of SATB1.

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"For example, USP47 reduced papillary thyroid carcinoma cell malignant behaviors by stabilizing special AT-rich sequence-binding protein-1 (SATB1) expression ( Luo et al., 2023 )."

37924851

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"Upregulation of ubiquitin carboxy‑terminal hydrolase 47 (USP47) in papillary thyroid carcinoma ex vivo and reduction of tumor cell malignant behaviors after USP47 knockdown by stabilizing SATB1 expression in vitro."

37564825

USP47 affects CTNNB1

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USP47 deubiquitinates CTNNB1.

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USP47 deubiquitinates CTNNB1. 10 / 10

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"USP47 deubiquitinates and stabilizes β-catenin to regulate human cancer cell proliferation [32]."

37311739

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"As a deubiquitinase, USP47 prevents β-catenin ubiquitination."

37001379

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"Additionally, DUBs USP14, USP15, USP47, and Fam/USP9X have been reported to prevent BETA-CATENIN turnover by inhibiting its Ub-proteasomal degradation"

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"Based on previous studies, USP47 impedes β-catenin ubiquitination and promotes cell proliferation ."

36813838

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"Thus, we concluded that PAM induces USP47 expression, which subsequently deubiquitinated β-catenin, leading to β-catenin stabilization in hDPCs."

36813838

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"Previous studies indicated that USP47 deubiquitylates β-catenin to inhibit its degradation (13)."

36749823

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"Specifically, USP47 deubiquitinates and stabilizes MAPK 25, DNA polymerase beta (Polbeta) 26, E-cadherin 27, beta-catenin 28, SNAIL 29, YAP 30, beta-Trcp 31, and katanin-p60 32."

33397955

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"USP47 also deubiquitinates β-catenin, which is crucial for tumorigenesis and animal development ."

37740002

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"USP47, known to deubiquitylate β-catenin [78] , was reported to be induced under hypoxic conditions [79] ."

31706510

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"Inhibition of USP47 by P5091 should contribute to the decreased β-catenin level, because a new study shows that USP47 deubiquitinates and stabilizes β-catenin as well [18] ."

28216017

USP47 binds CTNNB1.

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USP47 binds CTNNB1. 5 / 5

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"Although it has been reported that MAPK or beta-catenin interacts with USP47 in Drosophila or tumors XREF_BIBR, XREF_BIBR, USP47 knockdown does not affect the expression of MAPK or beta-catenin in CML cells."

33397955

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"USP47 directly interacts with β-catenin and affects Wnt/β-catenin signaling by deubiquitination of β-catenin."

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"Both USP15 and USP47 form a complex with beta-catenin leading to its deubiquitination and stabilization [248,249]."

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"The interaction between β-catenin and USP47 protein was weakened with the loss of FAM83H and increased with overexpression of FAM83H ( xref )."

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"USP47 directly interacts with beta-catenin and affects Wnt and beta-catenin signaling by deubiquitination of beta-catenin."

32486158

USP47 binds CTNNB1 and fbxw1. 1 / 1

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"Importantly, mutation of Arginine 434 to alanine abrogated the binding of β-Trcp with both USP47 and β-catenin ( xref , lane 10), indicating that the β-Trcp-USP47 association is comparable with other β-Trcp-substrate interactions."

19966869

USP47 activates CTNNB1.

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"Consistent with this result , we found that knockdown of USP47 detectably reduced the amount of beta-catenin ( Fig. 3A , S5A ) ."

36749823

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"The results indicate that USP47 induces a positive process in Wnt and beta-catenin signaling while increasing the stability of beta-catenin [XREF_BIBR, XREF_BIBR]."

32486158

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"In addition, USP47 deubiquitinates itself and positively regulates β-catenin stabilization [173]."

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USP47 deubiquitinates CDH1. 7 / 7

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"USP47 can be recruited to AJs and suppress the ubiquitination of E-cadherin, thus inhibiting its resultant proteasomal degradation"

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"As mentioned above, E-cadherin is deubiquitinated by USP47."

34604226

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"However, as mentioned above, E-cadherin can also be deubiquitinated and stabilized by USP47."

34604226

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"USP47 can be recruited to AJs and suppress the ubiquitination of E-cadherin, thus inhibiting its resultant proteasomal degradation."

29080116

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"On the other hand, KIFC3 transports to the junction the ubiquitin-specific protease 47 (USP47), which deubiquitinates E-cadherin, and thus prevents its degradation."

30395792

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"Specifically, USP47 deubiquitinates and stabilizes MAPK 25, DNA polymerase beta (Polbeta) 26, E-cadherin 27, beta-catenin 28, SNAIL 29, YAP 30, beta-Trcp 31, and katanin-p60 32."

33397955

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"While not being engaged in E-cadherin transport, KIFC3 reduces E-cadherin degradation by recruiting the ubiquitin-specific protease 47 (USP47, which deubiquitinates E-cadherin) to AJ, which prevents E-cadherin degradation and maintains the epithelial integrity (Meng et al., 2008; Sako-Kubota et al., 2014)."

35547823

USP47 activates CDH1.

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USP47 activates CDH1. 6 / 6

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"KIFC3 and USP47 depletion enhances formation of a unique E-cadherin fragment."

25253721

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"The enhancement of USP47 in colorectal cancer cells under hypoxic conditions induced the disassembly of E-cadherin and promoted EMT through deubiquitination of Snail."

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34604226

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"USP47 regulates base excision repair, suppresses E-cadherin degradation, and facilitates NLRP3 inflammasome activation to release IL-1β and IL-18 [30–32]."

35509185

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"Therefore, the modulation of E-cadherin by USP47 may be related to the cell states."

34604226

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"The enhancement of USP47 in colorectal cancer cells under hypoxic conditions induced the disassembly of CDH1 and promoted EMT through deubiquitination of SNAIL [ 21 ]."

32201364

USP47 decreases the amount of CDH1.

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USP47 decreases the amount of CDH1. 3 / 3

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"Knockdown of USP47 suppressed the expression of vimentin and N-cadherin and restored E-cadherin expression under hypoxic conditions."

29162839

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"Besides, USP47 knockout could increase E-cadherin expression and inhibited β-catenin expression in HCT116 cells, which is the reverse process of EMT."

34604226

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"Besides, knockdown of USP47 suppressed the expression of N-cadherin, vimentin and twist and restored E-cadherin expression ( Fig. 2 G)."

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USP47 bound to SNAI1 decreases the amount of CDH1. 1 / 1

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USP47 bound to SATB1 decreases the amount of CDH1. 1 / 1

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34604226

USP47 increases the amount of CDH1.

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"As the s, P5091 is also an elective dual-inhibitor of USP7 and USP47 that can suppress CRC cell growth and induces their apoptosis in vitro (An et al., 2017)."

37251574

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"USP47 knockdown attenuated myocardial cell I/R injury by inhibiting apoptosis."

32761659

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"Notably, selective dual inhibitors of USP7 and USP47 have been synthesized and induced accumulation of p53 and apoptosis in human cancer cell lines [XREF_BIBR]."

25481052

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"The silencing of USP47 increases the rate of apoptosis and the cytotoxic effects induced by chemotherapeutic agents in several strains, including osteosarcoma and breast cancer cells."

32201364

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"In agreement with this result, we observed that silencing of USP47 augments the apoptosis of MCF-7 breast cancer cells, which are known to be resistant to inhibition of NF-kappaB."

19966869

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"Also, knockdown of USP47 induced CRC cell apoptosis ( Fig. S1D )."

31233752

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"Besides, numerous results demonstrated that knockdown of USP47 increased the percentage of apoptosis and inhibited cell proliferation, which were consistence with our study."

37924851

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"USP47 facilitated osteosarcoma cell invasion and migration, and suppressed apoptosis."

33576460

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"In cultured CRC cells, knockdown of USP47 increased pyroptosis and apoptosis induced by chemotherapeutic doxorubicin."

34630087

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"A class of dual small molecule inhibitors of USP7 and USP47 has been identified to promote p53 activity and apoptosis in MM and B-cell leukemia cells in vitro and xenograft models."

33643919

USP47 activates apoptotic process.

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USP47 activates apoptotic process. 6 / 6

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"USP47 induced apoptosis was potently counteracted by the NF-kappaB inhibitor, and cytoplasmic NF-kappaB was increased and nuclear NF-kappaB was decreased."

32761659

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"However, USP47 deficiency inhibits SCF (β‐TrCP) degradation of Cdc25A, promotes apoptosis, and enhances the cytotoxicity of anticancer drugs.F‐box dysfunction will affect the survival of tumor cells."

39812503

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"USP47-deficient MEF cells isolated from a USP47 knockout mouse model revealed that USP47 knockout significantly increased the levels of ultraviolet-induced apoptosis (Peschiaroli et al., 2010)."

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"For instance, USP47 has been shown to promote apoptosis in rat cardiomyocytes following I/R injury by activating NF‐κB. 7 Additionally, USP7 enhances ferroptosis by activating the p53/TfR1 pathway after I/R in the rat heart."

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"Downregulation of USP47 decreased apoptosis induced by ischemia/reperfusion injury, increased survivin and cytoplasmic NF-κB, and decreased cleaved caspase-3 and nuclear NF-κB. NF-κB inhibitors could effectively inhibit USP47-induced apoptosis, increase cytoplasmic NF-κB, and decrease nuclear NF-κB. Luciferase reporter genes showed that USP47 promoted NF-κB promoter activity."

34604226

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"USP47 promotes apoptosis in rat myocardial cells after ischemia and reperfusion injury via NF-kappaB activation."

32761659

USP47 affects NLRP3

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USP47 activates NLRP3.

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USP47 activates NLRP3. 8 / 8

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"The DUBs USP7 and USP47 activate the NLRP3 inflammasome, but the specific mechanism is not clear (Palazón-Riquelme et al., 2018)."

36688154

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"In addition, inhibition of USP7 and USP47 blocks the NLRP3 inflammasome by preventing apeck-like protein containing a CARD (ASC) oligomerization and speck formation in macrophages [38]."

32650621

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"Inhibition of USP7 and USP47 impairs NLRP3 inflammasome activation in macrophages."

30206189

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"USP7 and USP47 also promote NLRP3 activation [55]."

38789414

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"For example, USP7 and USP47 promote the NLRP3 inflammasome by accelerating an apeck-like protein containing a CARD (ASC) oligomerization in macrophages [49]."

40108019

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"It is then possible that this process is regulated by K11 linked ubiquitin chains, yet it is also possible that USP7 and USP47 do not directly target NLRP3 and that an additional regulated protein, upstream of this process, is mediating this effect."

30206189

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"USP47 inhibition alleviated cardiac functions, and decreased infarct size, pro-inflammatory cytokines, NETs, NLRP3, and pyroptosis."

38850785

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"Moreover, USP7, USP47, and BRCC3 and ABRO can activate the NLRP3 inflammasome."

32850399

USP47 deubiquitinates NLRP3.

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USP47 deubiquitinates NLRP3. 7 / 7

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"A previous study revealed that USP7 and USP47 activated NLRP3 inflammasome by regulating ASC oligomerization, NLRP3 ubiquitination, and speck formation in macrophages, but remained unclear whether USP7 and USP47 contributed to NLRP3 deubiquitination directly or indirectly [32]."

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"However, it is not clear whether USP7 and USP47 directly contribute to NLRP3 deubiquitination or regulate the process somewhere upstream of NLRP3 inflammasome action [108]."

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"Conversely, deubiquitination of NLRP3 by USP7 and USP47 positively regulates inflammasome activation and USP47 is significantly downregulated in dopaminergic neurons from idiopathic PD patient post-mortem brain samples [XREF_BIBR, XREF_BIBR]."

33414510

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"A recent study has found that USP7 and USP47 are involved in the activation of inflammasomes in macrophages and play a direct role in the deubiquitination of NLRP3 [69]."

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"Both regulate various signaling pathways, such as the inflammasome pathway , WNT signaling , Hedgehog signaling , etc. USP7 and USP47 deubiquitinate NLRP3 and control the assembly and activation of inflammasomes in macrophages ."

37740002

➶

reach

"USP47 reversed the ubiquitination of NLRP3."

35678979

➶

reach

"ZNF883 targets miR-138-5p which negatively regulates ubiquitin-specific peptidase 47 (USP47), which reverses the ubiquitination of NLRP3 (Gong et al. 2022)."

40056236

USP47 ubiquitinates NLRP3.

| 3 1

USP47 ubiquitinates NLRP3. 4 / 4

| 3 1

➶

reach

"The relationship among ZNF883, miR-138-5p, and USP47 as well as the regulation of NLRP3 ubiquitination by USP47 was determined."

35678979

➶

reach

"Chemical inhibition of DUBs USP7 and USP47 increases the ubiquitination of NLRP3, prevents PYCARD oligomerization and speck formation, and blocks inflammasome formation [130]."

36551253

3 | 1 12

USP47 binds RPS2. 10 / 16

3 | 1 12

➶

sparser

"Localization data also showed that interaction between USP47 and RPS2 dissociated during ribosomal stress, as shown in xref a,b."

32370049

➶

sparser

"To verify the interaction of USP47 and RPS2, we performed immunoprecipitation with anti-Flag agarose after overexpressing HA-RPS2 and Flag-USP47."

32370049

➶

sparser

"Consistent with these results, we also found that endogenous RPS2 interacted with endogenous USP47 in cells ( xref b)."

32370049

➶

sparser

"As shown in xref a, USP47 interacted with RPS2 in cells."

32370049

➶

sparser

"Next, we examined the direct interaction between USP47 and RPS2 by a Glutathione S-transferase (GST) pull-down assay using the GST-USP47 477–934 protein that includes the ubiquitin-like (UBL) domain, as the UBL domain is known to mediate protein–protein interaction [ xref ]."

32370049

➶

sparser

"These results indicate that USP47 directly interacts with RPS2."

32370049

➶

reach

"As for USP47, the deficiency of UBLs impaired the binding between USP47 and RPS2, indicating that the UBLs of USP47 were also involved in substrate binding (Cho et al., 2020)."

34604226

➶

sparser

"Ribosomal Stress Inhibits the Interaction between USP47 and RPS2 and thus Increases p53."

32370049

➶

sparser

"On the contrary, the interaction between USP47 and RPS2 was not decreased by the addition of MDM2 ( xref d and xref )."

32370049

➶

sparser

"In addition, ribosomal stress decreases the interaction between RPS2 and USP47, and dissociated RPS2 can then inhibit MDM2 and subsequently lead to p53 stabilization."

32370049

USP47 deubiquitinates RPS2.

| 1 4

USP47 deubiquitinates RPS2. 5 / 5

| 1 4

➶

trips

"USP47 Promotes Tumorigenesis by Negative Regulation of p53 through Deubiquitinating Ribosomal Protein S2."

32370049

➶

reach

"Further mechanistic studies showed that USP47 deubiquitinated RPS2, thereby inhibiting interaction between RPS2 and MDM2, and alleviated RPS2-mediated MDM2 inhibition under normal conditions, thus inhibiting P53."

34604226

➶

reach

"Furthermore, we found that USP47 can function as a regulator of the MDM2-p53 axis by deubiquitinating ribosomal protein S2 (RPS2), e.g., ribosomal stress decreased the interaction between RPb2 and USP47, causing RPS2 to disassociate and inhibit MDM2, which subsequently lead to p53 stabilization."

37740002

➶

reach

"While USP7 stabilizes MDM2 directly, USP47 regulates MDM2 by deubiquitinating RPS2, suggesting that USP47 could be a delicate regulator of p53 in some stress responses."

37740002

➶

reach

"While USP47 and USP7 both regulate the MDM2–p53 pathway, USP7 stabilizes MDM2 directly, while USP47 regulates MDM2 by deubiquitinating RPS2 ."

37740002

SNAI1 affects USP47

| 12 9

SNAI1 binds USP47.

| 10 9

USP47 binds SNAI1. 10 / 18

| 10 8

➶

reach

39998882

➶

reach

34604226

➶

sparser

"Co-immunoprecipitation (Co-IP) assays using a USP47 antibody confirmed a physical interaction between USP47 and Snai1 ( xref )."

39998882

➶

sparser

"Taken together, these results indicate that USP47 interacts directly with Snail and impedes its ubiquitination and subsequent proteasomal degradation."

29162839

➶

sparser

"USP47 binds to Snail and triggers its deubiquitination."

29162839

➶

sparser

"However, under hypoxic conditions, USP47 binding to Snail was markedly enhanced (Fig. xref )."

29162839

➶

sparser

"To visualize the interaction between Snail and USP47, we stained the DLD-1 cells with fluorescent probes detecting USP47 (red) and Snail (green)."

29162839

➶

reach

"Notably, USP47 interacts directly with Snail and enhances its stability."

29162839

➶

reach

"USP47 binds to Snail and triggers its deubiquitination."

29162839

➶

reach

"However, under hypoxic conditions, USP47 binding to Snail was markedly enhanced."

29162839

SNAI1 binds SATB1 and USP47. 1 / 1

| 1

➶

sparser

34604226

SNAI1 activates USP47.

| 2

SNAI1 activates USP47. 2 / 2

| 2

➶

reach

"USP47 is an ubiquitin peptidase involved in hypoxia-induced EMT by SNAI1 deubiquitination and stabilization [187]."

37587481

➶

reach

"The decrease in Snai1 protein levels caused by knocking down USP47 was rescued upon treatment with the proteasome inhibitor MG132 (Figure 6E)."

39998882

USP47 affects Cell Survival

| 2 14

USP47 activates Cell Survival.

| 2 11

USP47 activates Cell Survival. 10 / 16

| 2 11

➶

reach

"The CCK-8 assay results demonstrated that USP47 knockdown significantly reduced cell viability compared with the shNC group, which was significantly reversed by co-transfection with miR-454 knockdown in 5-8F/DDP and SUNE-1 and DDP cells (XREF_FIG)."

33576460

➶

reach

"Silencing of USP47 decreases cell survival and augments the cytotoxic effects of antitumor drugs on a variety of tumor cells, including osteosarcoma and breast cancer cell lines."

19966869

➶

reach

"Compared with the shNC group, USP47 knockdown significantly suppressed NPC cell viability and DDP resistance, which was significantly reversed by co-transfection with miR-454 inhibitor."

33576460

➶

reach

"Silencing of USP47 inhibits cell survival and sensitizes cells to chemotherapic agent induced apoptosis."

19966869

➶

reach

"As shown in Fig. 4 A , USP47 knockdown by either sh- USP47 –1 or sh- USP47 –2 suppressed cell viability in HaCaT cells under 0 and 10 Gy irradiation compared to the control sh-NC group."

37924851

➶

eidos

"Compared with the shNC group , USP47 knockdown significantly suppressed NPC cell viability and DDP resistance , which was significantly reversed by co-transfection with miR-454 inhibitor ."

33576460

➶

reach

"Another study of USP47 in gastric cancer cell lines showed that USP47 silencing or depletion leads to decreasing cell survival and contributes to anti-proliferative effects of cancer drugs [ 50 ]."

32201364

➶

reach

"USP47 has also been described as an interaction partner for the ubiquitin E3 ligase complex, beta-TrCP, with the authors of this study showing that silencing of USP47 expression inhibits cell survival and sensitises cells to chemotherapeutic agent induced apoptosis [XREF_BIBR]."

23712866

➶

reach

"It was recently demonstrated that silencing of USP47 inhibits cell survival and sensitizes cells to DNA damaging agents (Peschiaroli et al., 2010)."

21362556

➶

reach

"Additionally, the utilization of AZD6738 (50nM), an ATR pathway inhibitor, partially inhibits USP47 knockdown induced suppression of cell viability in CML cells."

33397955

USP47 inhibits Cell Survival.

| 3

USP47 inhibits Cell Survival. 3 / 3

| 3

➶

reach

"Notably, genetic or siRNA mediated depletion of USP47 decreases cell survival and augments the anti-proliferative effect of anticancer drugs, providing a novel potential target for anticancer therapies."

19966869

➶

reach

"Notably, genetic or siRNA mediated depletion of USP47 induced accumulation of Cdc25A, decreased cell survival and augmented the cytotoxic effects of anticancer drugs."

19966869

➶

reach

"Despite the functional importance and implications of SATB1 expression in cancer progression, the mechanisms governing its cellular levels remain largely unknown.Ubiquitin-specific peptidase 47 (USP47[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

30742943

USP47 affects epithelial to mesenchymal transition

| 18

USP47 activates epithelial to mesenchymal transition. 10 / 18

| 18

➶

reach

"To test the hypothesis that SATB1 is responsible for the USP47-induced EMT in colon cancer cells, we generated USP47-knockout HCT116 cells and overexpressed SATB1 in this cell line."

30742943

➶

reach

"The enhancement of USP47 in colorectal cancer cells under hypoxic conditions induced the disassembly of E-cadherin and promoted EMT through deubiquitination of Snail."

29162839

➶

reach

"Silencing of USP47 accelerated the proteasomal degradation of Snail and inhibited EMT."

29162839

➶

reach

34604226

➶

reach

"However, the relationship between EMT mediated by USP47 and E-cadherin was not explored in this study."

34604226

➶

reach

"USP47 is an ubiquitin peptidase involved in hypoxia-induced EMT by SNAI1 deubiquitination and stabilization [187]."

37587481

➶

reach

"Hypoxia-mediated induction of USP47 promotes EMT in colorectal cancer by transcriptional regulation of SNAIL expression [272]."

34814083

➶

reach

"Here we report that upregulation of USP47 under hypoxic conditions stimulates EMT in CRC cells and subsequently their metastatic potential."

29162839

➶

reach

"There were growing evidences supporting that USP47 promotes tumorigenesis, malignancies cell proliferation, drug resistance and epithelial-mesenchymal transition ( Hou et al., 2021 ; Park et al., 2022[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

37924851

➶

reach

"Furthermore, we identify Snai1 as a deubiquitination target of USP47, explaining USP47-dependent activation of the epithelial-mesenchymal transition pathway and tumor progression."

39998882

RPS2 affects USP47

3 | 1 12

USP47 binds RPS2. 10 / 16

3 | 1 12

➶

sparser

"Localization data also showed that interaction between USP47 and RPS2 dissociated during ribosomal stress, as shown in xref a,b."

32370049

➶

sparser

"To verify the interaction of USP47 and RPS2, we performed immunoprecipitation with anti-Flag agarose after overexpressing HA-RPS2 and Flag-USP47."

32370049

➶

sparser

"Consistent with these results, we also found that endogenous RPS2 interacted with endogenous USP47 in cells ( xref b)."

32370049

➶

sparser

"As shown in xref a, USP47 interacted with RPS2 in cells."

32370049

➶

sparser

32370049

➶

sparser

"These results indicate that USP47 directly interacts with RPS2."

32370049

➶

reach

"As for USP47, the deficiency of UBLs impaired the binding between USP47 and RPS2, indicating that the UBLs of USP47 were also involved in substrate binding (Cho et al., 2020)."

34604226

➶

sparser

"Ribosomal Stress Inhibits the Interaction between USP47 and RPS2 and thus Increases p53."

32370049

➶

sparser

"On the contrary, the interaction between USP47 and RPS2 was not decreased by the addition of MDM2 ( xref d and xref )."

32370049

➶

sparser

"In addition, ribosomal stress decreases the interaction between RPS2 and USP47, and dissociated RPS2 can then inhibit MDM2 and subsequently lead to p53 stabilization."

32370049

USP47 affects YAP1

1 | 5 8

USP47 binds YAP1.

1 | 2 8

USP47 binds YAP1. 10 / 11

1 | 2 8

➶

sparser

"In co-immunoprecipitation (Co-IP) assays, recombinant USP47 could pull-down YAP and vice versa , suggesting that USP47 and YAP physically interact with each other (Fig. xref A)."

31748975

➶

biogrid

No evidence text available

29891922

➶

sparser

"In summary, we uncovered an USP47-YAP signaling axis in regulating the development of CRC."

31748975

➶

reach

"USP7 and USP47 share substrates such as the Yes-associated protein (YAP), which functions as the main effector of the Hippo pathway ."

37740002

➶

sparser

"Taken together, these results indicate that YAP physically interacts with USP47, and USP47 serves as a DUB for YAP."

31748975

➶

sparser

"In addition, USP47 interacts with YAP, promotes YAP transcriptional activity and protein stability, and is the interactor for β-Trcp, an E3 ubiquitin ligase, leading to NLR family pyrin domain-containing (NLRP)3 proteasomal degradation via Lys27-linked polyubiquitination at Lys380."

35145062

➶

sparser

"USP47 interacts with YAP, facilitating its deubiquitination and thereby stabilizing YAP."

41258572

➶

reach

"It has also been observed by Pan et al that USP47 can interact with, deubiquitinate, and stabilize YAP to activate the pro-regenerative Hippo signaling pathway [ 46 ]."

37487274

➶

sparser

"This suggests that the USP47-YAP signalling axis in the Hippo pathway may mediate NLRP3 activation and regulate immune and inflammatory responses."

35145062

➶

sparser

"To verify this hypothesis, we first tested the interaction between USP47 and YAP."

31748975

USP47 deubiquitinates YAP1.

| 3

USP47 deubiquitinates YAP1. 3 / 3

| 3

➶

reach

"The deubiquitination enzymes ubiquitin specific peptidase 10 (USP10), ubiquitin specific peptidase 47 (USP47), OUT deubiquitinase, ubiquitin aldehyde binding 2 (OTUB2), and eukaryotic translation initiation factor 3 subunit H (EIF3H) deubiquitinated YAP1 to maintain protein stability [61, 62, 63]."

37005481

➶

reach

"Another three DUBs, ubiquitin carboxyl-terminal hydrolase 10 (USP10), otubain-2 (OTUB2) and ubiquitin carboxyl-terminal hydrolase 47 (USP47) deubiquitinate YAP/TAZ, playing important roles in hepatocellular carcinoma, breast cancer and colorectal cancer, respectively21 32 33."

35024322

➶

reach

"For instance, USP9X, ubiquitin-specific peptidase 47 (USP47), and OTU deubiquitinase ubiquitin aldehyde binding 1 (OTUB1) directly deubiquitinate YAP [33–35]."

37743467

SATB1 affects USP47

3 | 2 9

SATB1 binds USP47. 10 / 13

3 | 2 8

➶

sparser

"Together with our findings that SATB1 interacted with USP47, these results indicate that deubiquitinase activity is required for USP47 to remove ubiquitin from SATB1."

30742943

➶

sparser

37564825

➶

sparser

"To explore the roles of USP47-SATB1 interactions in CRC metastasis, we examined cell migration and invasion."

30742943

➶

sparser

"As seen in Fig. 2 E, this mutant failed to inhibit the ubiquitination of SATB1 protein without affecting USP47 interaction with SATB1 ( Fig. 2 Eand F)."

30742943

➶

biogrid

No evidence text available

30742943

➶

sparser

"USP47 interacted with SATB1 through its catalytic core domain and antagonized Smurf2-mediated SATB1 degradation."

34604226

➶

biogrid

No evidence text available

30742943

➶

biogrid

No evidence text available

30742943

➶

sparser

"Here, we demonstrate that USP47 interacts with SATB1 to mediate its ubiquitination and positively regulate its stability."

30742943

➶

reach

30742943

SNAI1 binds SATB1 and USP47. 1 / 1

| 1

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sparser

34604226

PNLIP affects USP47

| 8 6

PNLIP binds USP47.

| 3 4

USP47 binds PNLIP. 7 / 7

| 3 4

➶

reach

"Next, the binding of USP47 and PTL was explored using cellular thermal shift assay (CETSA), a newly developed method to evaluate drug binding to target proteins in cells based on the biophysical princ[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

34030041

➶

reach

"To assess whether PTL binds to USP47, CETSA was firstly undertaken in HCT116 cell lysates."

34030041

➶

reach

"Compared with DMSO treatment, PTL incubation resulted in an obvious thermostability of USP47 at different temperatures ( Fig. 3 E) and different doses ( Fig. 3 F), confirming the binding of PTL to USP[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

34030041

➶

sparser

34030041

➶

sparser

"To assess whether PTL binds to USP47, CETSA was firstly undertaken in HCT116 cell lysates."

34030041

➶

sparser

"Next, in order to investigate whether PTL interacts with USP47 in intact cells, HCT116 cells treated with PTL or DMSO were collected, lysed and heated."

34030041

➶

sparser

34030041

PNLIP inhibits USP47.

| 4 2

PNLIP inhibits USP47. 6 / 6

| 4 2

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reach

"In this study we found PTL effectively inhibited USP47 and CRC, which is also an inhibitor of USP7 we previously reported [ 22 ]."

34030041

➶

sparser

"In this study we found PTL effectively inhibited USP47 and CRC, which is also an inhibitor of USP7 we previously reported [ 22 ]."

34030041

➶

reach

"As shown in Fig. 3 A–B, PTL inhibited USP47 activity in dose- and time-dependent manner."

34030041

➶

reach

"In summary, PTL inhibited USP47 and improved the thermostability of USP47, indicating PTL was a novel USP47 inhibitor.Next, we explored whether the USP47 inhibitor PTL effectively inhibited CCSCs."

34030041

➶

reach

"These results indicated USP7 might not regulate CCSCs, and PTL modulates CCSCs preferentially by antagonizing USP47.In this study, we demonstrated that USP47 regulates CCSCs, and as an inhibitor of US[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

34030041

➶

sparser

"In summary, PTL inhibited USP47 and improved the thermostability of USP47, indicating PTL was a novel USP47 inhibitor."

34030041

PNLIP activates USP47.

| 1

PNLIP activates USP47. 1 / 1

| 1

➶

reach

"In summary, PTL inhibited USP47 and improved the thermostability of USP47, indicating PTL was a novel USP47 inhibitor.Next, we explored whether the USP47 inhibitor PTL effectively inhibited CCSCs."

34030041

USP7 affects USP47

| 5 8

USP7 binds USP47.

| 1 8

USP7 binds USP47. 7 / 7

| 1 6

➶

sparser

"Given the association of USP7 and USP47 with the MDM2-p53 pathway xref , xref , we investigated whether the impact of USP7 and USP47 on cancer cell growth is dependent on p53."

37740002

➶

sparser

"Furthermore, activated inflammasomes also regulate USP7 and USP47 activity, which forms a positive feedback pathway for resistance to pathogen infection ( xref )."

34966378

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sparser

"This study showed that chemical inhibition of USP7 and USP47 blocked inflammasome formation and that although both DUBs contributed to inflammasome activation, its activation was majorly impaired when both USP7 and USP47 were not present."

31679183

➶

sparser

"However, the biggest potential limitation that emerges from this paper is that all of the known USP7 inhibitors (until recently) also interact with USP47 ( Altun et al., 2011 )."

29272698

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sparser

"P22077, a USP7 inhibitor, has also been shown to inhibit the NF-κB and MAPKs pathways and exert important anti-inflammatory effects in vitro and in vivo by promoting K48-linked ubiquitination and TRAF6 degradation. xref Palazon-Riquelme et al xref found that in macrophages, P22077 prevents the formation and activation of inflammatory vesicles by inhibiting USP7 and USP47 and reducing ASC oligomerization and speckling."

38529479

➶

sparser

"Whether USP7 and USP47 directly interact with NLRP3 receptor or with other inflammasome component was not investigated."

31679183

➶

reach

"However, there is no evidence of direct interaction between USP7, USP47, and NLRP3 [XREF_BIBR]."

33138315

USP7 binds USP47 and SHBG. 1 / 1

| 1

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sparser

"Strikingly, treatment of LPS‐primed MDMs with nigericin induced a dramatic increase in the proportion of ABP‐bound USP7 and USP47 (Fig xref A)."

30206189

USP7 binds USP47 and NLRP3. 1 / 1

| 1

➶

sparser

"However, there is no evidence of direct interaction between USP7, USP47, and NLRP3 [ xref ]."

33138315

USP7 inhibits USP47.

| 2

USP7 inhibits USP47. 2 / 2

| 2

➶

reach

"Due to the multifaceted roles of USP7, many inhibitors have been developed targeting USP7, such as P022077, HBX 41,108, HBX-19,818, HBX-28,258, P5091, Cpd 14 and P22077, in which the latter two molecules also inhibit USP47."

28279784

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reach

28031783

USP7 activates USP47.

| 2

USP7 activates USP47. 2 / 2

| 2

➶

reach

"By depleting USP7 and USP47 in macrophage cells, USP7 and USP47 activation of inflammasomes was reduced."

37300757

➶

reach

"These results suggest that the C-terminal UBL domains of hUSP47 are similar to USP7 in that they support the DUB activity of the USP47 catalytic domain."

37740002

USP47 affects YBX1

3 | 6 4

USP47 binds YBX1.

3 | 2 4

USP47 binds YBX1. 9 / 9

3 | 2 4

➶

sparser

"It was identified that YB-1 can interact with USP47 in K562 and primary CML cells (Fig. xref )."

33397955

➶

sparser

"The results show that USP47 interacts with the C-terminal domain (CTD) domain of YB-1 (Fig. xref )."

33397955

➶

reach

"Interestingly, the interaction between YB-1 and USP47 is not affected by the ubiquitination status of YB-1, as the mutation of lysine residues (Lys137, -164, and -170) abrogates the ubiquitination of YB-1, but not its interaction with USP47."

33397955

➶

sparser

"USP47 also binds to downstream Y-box binding protein 1 and protects it from proteasome degradation, making it conducive for DNA damage repair to occur in CML cells."

38947742

➶

sparser

"Interestingly, the interaction between YB-1 and USP47 is not affected by the ubiquitination status of YB-1, as the mutation of lysine residues (Lys137, −164, and −170) abrogates the ubiquitination of YB-1, but not its interaction with USP47 (Fig. xref )."

33397955

➶

reach

"USP47 also binds to downstream Y-box binding protein 1 and protects it from proteasome degradation, making it conducive for DNA damage repair to occur in CML cells."

38947742

➶

| 3 4

➶

eidos

"Finally , we show that depletion of USP47 induces p53 and therefore inhibits cell proliferation , colony formation , and tumor progression in cancer cell lines and a mouse xenograft model ."

32370049

➶

eidos

"As shown in Figure 2a , the depletion of USP47 increased the p53 level but not in the presence of MG132 ."

32370049

➶

reach

"Notably, selective dual inhibitors of USP7 and USP47 have been synthesized and induced accumulation of p53 and apoptosis in human cancer cell lines [XREF_BIBR]."

25481052

➶

eidos

"Finally , we provide evidence that USP47 has oncogenic potential , and depletion of USP47 induces p53 by regulation of RPS2 and , therefore , inhibits cell proliferation , colony formation , and tumor progression in cancer cell lines and a mouse xenograft model in a p53-dependent manner ."

32370049

➶

reach

"Compound 1, a selective inhibitor of USP7 and USP47 with moderate potency, demonstrates inhibition of USP7 in cells and induces elevated p53 and apoptosis in cancer cell lines."

24900381

➶

reach

"A class of dual small molecule inhibitors of USP7 and USP47 has been identified to promote p53 activity and apoptosis in MM and B-cell leukemia cells in vitro and xenograft models."

33643919

➶

reach

"As shown in Fig. 1, USP47 and USP7 inhibit cancer cell growth in a p53-dependent manner."

37740002

USP47 decreases the amount of TP53.

| 2

USP47 decreases the amount of TP53. 2 / 2

| 2

➶

| 1

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reach

34604226

USP47 affects Neoplasms

| 2 11

USP47 activates Neoplasms.

| 2 10

USP47 activates Neoplasms. 10 / 12

| 2 10

➶

reach

"Knockdown of USP47 induces CDC25A accumulation and the inhibition of tumor growth."

37686524

➶

reach

"Furthermore, xenograft assays demonstrated that silencing USP47 can inhibit LUSC tumor growth in vivo."

35254655

➶

reach

"Consistent with in vitro results, USP47 knockdown drastically inhibited tumor growth in vivo (Figure 2F and Supplemental Figure 6F)."

39998882

➶

reach

"Conversely, USP47 overexpression markedly accelerated tumor growth, yielding larger tumors and higher weights (Supplemental Figure 7, I and J)."

39998882

➶

eidos

"Thus , the findings here indicate that USP47 can promote tumor proliferation by regulating levels of p53 ."

32370049

➶

reach

"Ubiquitin-specific peptidase 47 expression is elevated in many tumors and is involved in multiple expression regulating mechanisms, including transcriptional, post-transcriptional, and post-translational modifications."

34604226

➶

reach

"Furthermore, we identify Snai1 as a deubiquitination target of USP47, explaining USP47-dependent activation of the epithelial-mesenchymal transition pathway and tumor progression."

39998882

➶

reach

"Yu et al also reported that USP47 and SMURF2 can mediate CC cell proliferation and tumor progression by reversibly manipulating SATB1 ubiquitination."

37158531

➶

reach

"Overexpression of USP47 could reduce the tumor inhibition induced by LINC00668 knockout (Hu et al., 2019)."

34604226

➶

reach

"USP47 subsequently stabilizes Snail by deubiquitination and promotes EMT and tumor metastasis (35)."

35616129

USP47 inhibits Neoplasms.

| 1

USP47 inhibits Neoplasms. 1 / 1

| 1

➶

reach

"USP47 deficiency in mice modulates tumor infiltrating immune cells and enhances antitumor immune responses in prostate cancer."

38832955

USP47 affects KIFC3

2 | 6 2

USP47 binds KIFC3.

2 | 5 2

KIFC3 binds USP47. 9 / 12

2 | 5 2

➶

biogrid

No evidence text available

25253721

➶

biogrid

No evidence text available

25253721

➶

reach

"Our detailed analysis of the function of the KIFC3 and USP47 complex suggests that it suppresses E-cadherin degradation and internalization."

25253721

➶

reach

"Double staining for E-cadherin and endogenous KIFC3 or USP47 also showed their overlapping distributions (XREF_FIG), indicating that the KIFC3 and USP47 complex accumulates at the AJs or zonula adherens (ZA), the epithelial form of an AJ."

25253721

➶

reach

"p120-catenin may serve E-cadherin stabilization by leading the KIFC3 and USP47 complex to these junctions."

25253721

➶

reach

"Of note, the KIFC3 and USP47 complex was detected throughout the cytoplasm."

25253721

➶

sparser

"Here we find that KIFC3 binds the ubiquitin-specific protease USP47, a protease that removes ubiquitin chains from substrates and hence inhibits proteasome-mediated proteolysis, and recruits it to AJs."

25253721

➶

reach

"Thus, the stabilizing role of p120-catenin on E-cadherin might also be explained by its involvement in directing the KIFC3–USP47 complex to adheren junctions [32]."

39833727

➶

sparser

"Thus, the stabilizing role of p120-catenin on E-cadherin might also be explained by its involvement in directing the KIFC3–USP47 complex to adheren junctions [ xref ]."

39833727

USP47 activates KIFC3.

| 1

USP47 activates KIFC3. 1 / 1

| 1

➶

reach

"Expression of murine KIFC3-FLAG in cells treated with KIFC3 siRNA or an siRNA resistant mutant of USP47-FLAG in cells treated with USP47 siRNA suppressed the generation of AbH-108-positive E-cadherin vesicles (Supplemental Figure S2)."

25253721

USP47 affects FBXW11

11 | 2

FBXW11 binds USP47. 10 / 13

11 | 2

➶

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No evidence text available

22017875

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No evidence text available

25900982

➶

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No evidence text available

25332235

➶

biogrid

No evidence text available

25332235

➶

biogrid

No evidence text available

35831314

➶

biogrid

No evidence text available

19966869

➶

biogrid

No evidence text available

19615732

➶

sparser

"Recently, the involvement of βTrCP-bound deubiquitylase enzyme USP47 has been described in HH signaling."

32531973

➶

sparser

"In particular, ERAP1 was demonstrated to catalyze beta-transducin repeat containing E3 ubiquitin protein ligase (βTrCP)-degradation by directly interacting with the βTrCP-bound deubiquitylase enzyme USP47."

40226591

➶

biogrid

No evidence text available

28514442

P22077 affects USP47

| 1 12

P22077 inhibits USP47. 10 / 13

| 1 12

➶

reach

"It has been reported that P22077, a selective ubiquitin-specific protease 7/47 (USP7/USP47) inhibitor, inhibited the proliferation of HIV virus by inhibiting the function of USP47 [27]."

31982568

➶

reach

"P22077, a USP7 inhibitor, has also been shown to inhibit the NF-κB and MAPKs pathways and exert important anti-inflammatory effects in vitro and in vivo by promoting K48-linked ubiquitination and TRAF6 degradation.19 Palazon-Riquelme et al9 found that in macrophages, P22077 prevents the formation and activation of inflammatory vesicles by inhibiting USP7 and USP47 and reducing ASC oligomerization and speckling."

38529479

➶

reach

"We observed that P22077 inhibited IL-1beta, IL-18 and caspase-1 cleavage and release, as well as pyroptotic cell death (Fig XREF_FIG A-D), indicating that USP7 and/or the closely related DUB USP47 are important for inflammasome activation."

30206189

➶

reach

"In this regard, P22077 was reported to inhibit Usp47."

28246399

➶

reach

"P22077 and PR-619 are inhibitors for both USP7 and USP47 [49] ."

37001379

➶

reach

"P22077 may promote the degradation of USP47 by inhibiting the auto-deubiquitinating process of USP47 28, which is supported by the finding that MG132 reverses P22077 induced USP47 degradation."

33397955

➶

reach

"For example, P22077 and P5091 also inhibited USP47 with similar potency [10,77,83,87] ."

34474304

➶

reach

"P22077 and PR-619 are specific inhibitors of USP7 and USP47, which inhibit Gag processing and reduce the release of infectious virions in HIV-1 infection [60]."

34803084

➶

reach

"P22077-mediated inhibition of USP47 exerts cytotoxicity in leukemia cells both in vitro and in vivo [246].7.2 DUBs and cell cycle regulation."

34814083

➶

eidos

"Inhibiting USP47 by P22077 exerts cytotoxicity to CML cells with or without TKI resistance in vitro and in vivo ."

33397955

FBXW11 affects USP47

11 | 2

FBXW11 binds USP47. 10 / 13

11 | 2

➶

biogrid

No evidence text available

22017875

➶

biogrid

No evidence text available

25900982

➶

biogrid

No evidence text available

25332235

➶

biogrid

No evidence text available

25332235

➶

biogrid

No evidence text available

35831314

➶

biogrid

No evidence text available

19966869

➶

biogrid

No evidence text available

19615732

➶

sparser

"Recently, the involvement of βTrCP-bound deubiquitylase enzyme USP47 has been described in HH signaling."

32531973

➶

sparser

40226591

➶

biogrid

No evidence text available

28514442

USP47 affects Wnt

| 3 8

USP47 activates Wnt. 10 / 12

| 3 8

➶

reach

"USP47 also increases Wnt signaling through deubiquitination of β-catenin, an important EMT biomarker, in A549 lung and PC3 prostate cancer cells [ 20 ]."

32201364

➶

eidos

"In human cells , knockdown of USP47 inhibited Wnt reporter activity , and USP47 acted downstream of the beta-catenin destruction complex ."

36749823

➶

reach

"USP47 also augments Wnt signaling through deubiquitination of beta-catenin in A549 lung and PC3 prostate cancer cells 18."

29162839

➶

eidos

"We found that USP47 knockdown inhibited Wnt signaling in HCT116 cells ( Fig. 3D ) ( 18 ) ."

36749823

➶

reach

"In human cells, knockdown of USP47 inhibited Wnt reporter activity, and USP47 acted downstream of the beta-catenin destruction complex."

36749823

➶

eidos

"Given that USP47 opposes the activity of XIAP , a positive Wnt regulator , by deubiquitylating its substrate , Gro / TLE , the finding that USP47 promotes Wnt signaling was unexpected ."

36749823

➶

reach

"The results indicate that USP47 induces a positive process in Wnt and beta-catenin signaling while increasing the stability of beta-catenin [XREF_BIBR, XREF_BIBR]."

32486158

➶

reach

"We found that USP47 knockdown inhibited Wnt signaling in HCT116 cells (Fig. 3D) (18)."

36749823

➶

reach

"Future studies will focus on whether high amounts of USP47 potentiate Wnt signaling in pancreatic cancers with RNF43 mutations."

36749823

➶

reach

"Given that USP47 opposes the activity of XIAP, a positive Wnt regulator, by deubiquitylating its substrate, Gro/TLE, the finding that USP47 promotes Wnt signaling was unexpected."

36749823

KIFC3 affects USP47

2 | 5 2

KIFC3 binds USP47. 9 / 12

2 | 5 2

➶

biogrid

No evidence text available

25253721

➶

biogrid

No evidence text available

25253721

➶

reach

"Our detailed analysis of the function of the KIFC3 and USP47 complex suggests that it suppresses E-cadherin degradation and internalization."

25253721

➶

reach

25253721

➶

reach

"p120-catenin may serve E-cadherin stabilization by leading the KIFC3 and USP47 complex to these junctions."

25253721

➶

reach

"Of note, the KIFC3 and USP47 complex was detected throughout the cytoplasm."

25253721

➶

sparser

25253721

➶

reach

"Thus, the stabilizing role of p120-catenin on E-cadherin might also be explained by its involvement in directing the KIFC3–USP47 complex to adheren junctions [32]."

39833727

➶

sparser

"Thus, the stabilizing role of p120-catenin on E-cadherin might also be explained by its involvement in directing the KIFC3–USP47 complex to adheren junctions [ xref ]."

39833727

YAP1 affects USP47

1 | 2 8

USP47 binds YAP1. 10 / 11

1 | 2 8

➶

sparser

"In co-immunoprecipitation (Co-IP) assays, recombinant USP47 could pull-down YAP and vice versa , suggesting that USP47 and YAP physically interact with each other (Fig. xref A)."

31748975

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biogrid

No evidence text available

29891922

➶

sparser

"In summary, we uncovered an USP47-YAP signaling axis in regulating the development of CRC."

31748975

➶

reach

"USP7 and USP47 share substrates such as the Yes-associated protein (YAP), which functions as the main effector of the Hippo pathway ."

37740002

➶

sparser

"Taken together, these results indicate that YAP physically interacts with USP47, and USP47 serves as a DUB for YAP."

31748975

➶

sparser

35145062

➶

sparser

"USP47 interacts with YAP, facilitating its deubiquitination and thereby stabilizing YAP."

41258572

➶

reach

"It has also been observed by Pan et al that USP47 can interact with, deubiquitinate, and stabilize YAP to activate the pro-regenerative Hippo signaling pathway [ 46 ]."

37487274

➶

sparser

"This suggests that the USP47-YAP signalling axis in the Hippo pathway may mediate NLRP3 activation and regulate immune and inflammatory responses."

35145062

➶

sparser

"To verify this hypothesis, we first tested the interaction between USP47 and YAP."

31748975

USP47 affects fbxw1

| 8 3

USP47 binds fbxw1.

| 5 3

USP47 binds fbxw1. 7 / 7

| 5 2

➶

sparser

"These data showed that USP47 interacts specifically with β-Trcp."

19966869

➶

sparser

"Unexpectedly, the USP47 (S914/918A) mutant bound endogenous β-Trcp with the same efficiency as the wild-type protein ( xref , lanes 2 and 3)."

19966869

➶

reach

"To confirm the specific binding between USP47 and beta-Trcp, we screened 19 human F-box proteins."

19966869

➶

reach

"These data showed that USP47 interacts specifically with beta-Trcp."

19966869

➶

reach

"To analyze whether the binding between beta-Trcp and USP47 requires the WD40-repeat domain and an intact Arg434, we performed co-immunoprecipitation experiments using different beta Trcp mutants (all FLAG tagged)."

19966869

➶

reach

"To analyze whether the physical interaction between USP47 and beta-Trcp resulted into a proteasome dependent degradation of USP47, we first analyzed the effect of the proteasome inhibitor MG132 on USP47 protein levels."

19966869

➶

reach

"The specificity of the binding between USP47 and beta-Trcp and the similarity of the phenotypes produced by targeting USP47 or beta-Trcp suggested to us that USP47 could be a regulator of beta-Trcp activity."

19966869

USP47 binds CTNNB1 and fbxw1. 1 / 1

| 1

➶

sparser

19966869

USP47 inhibits fbxw1.

| 2

USP47 inhibits fbxw1. 2 / 2

| 2

➶

reach

"ERAP1 promotes Hedgehog dependent tumorigenesis by controlling USP47 mediated degradation of beta TrCP."

32070165

➶

reach

"ERAP1 promotes Hedgehog dependent tumorigenesis by controlling USP47 mediated degradation of betaTrCP."

31341163

USP47 increases the amount of fbxw1.

| 1

USP47 increases the amount of fbxw1. 1 / 1

| 1

➶

reach

"USP47 knockdown significantly reduced the expression of beta-transducin repeat-containing protein (BTRC), serine/threonine-protein kinase akt-1 (AKT1), Klotho, fibroblast growth factor (FGF23), and matrix Gla protein (MGP) in RASMCs after high-phosphorus treatment."

35509185

USP47 affects CDC25A

| 11

USP47 activates CDC25A.

| 5

USP47 activates CDC25A. 5 / 5

| 5

➶

reach

39812503

➶

reach

"Notably, genetic or siRNA mediated depletion of USP47 induced accumulation of Cdc25A, decreased cell survival and augmented the cytotoxic effects of anticancer drugs."

19966869

➶

reach

"Depletion of USP47 induced accumulation of Cdc25A and decreased cell survival [XREF_BIBR]."

28122328

➶

reach

"Moreover, USP47 depletion induced upregulation of Cdc25A to similar levels as the silencing of betaTrcp (XREF_SUPPLEMENTARY)."

19966869

➶

reach

"Moreover, we found that in response to hydroxyurea, Cdc25A was degraded in USP47 depleted cells to similar levels as in control cells (XREF_FIG), implying a transcriptional regulation of Cdc25A induced by USP47 depletion."

19966869

USP47 increases the amount of CDC25A.

| 3

USP47 increases the amount of CDC25A. 3 / 3

| 3

➶

reach

"To verify this, we performed a quantitative real-time PCR and we found that USP47 but not beta-Trcp depletion increases Cdc25A mRNA levels (XREF_FIG)."

19966869

➶

| 4 2

SOX9 activates USP47. 6 / 6

| 4 2

➶

reach

"Notably, hypoxia induced USP47 upregulation was mediated by Sox9."

29162839

➶

reach

"In colorectal cancer cells, USP47 is activated by the hypoxia-mediated Sox9."

35616129

➶

reach

"Studies also reveal that Cldn7 deficiency induces stem-like properties in CRC via SOX9-mediated Wnt/β-catenin signaling [15], while hypoxia-driven SOX9 upregulates USP47, facilitating epithelial-mesenchymal transition (EMT) [16]."

40140224

➶

| 5 3

USP47 binds fbxw1. 7 / 7

| 5 2

➶

sparser

"These data showed that USP47 interacts specifically with β-Trcp."

19966869

➶

sparser

"Unexpectedly, the USP47 (S914/918A) mutant bound endogenous β-Trcp with the same efficiency as the wild-type protein ( xref , lanes 2 and 3)."

19966869

➶

reach

"To confirm the specific binding between USP47 and beta-Trcp, we screened 19 human F-box proteins."

19966869

➶

reach

"These data showed that USP47 interacts specifically with beta-Trcp."

19966869

➶

reach

19966869

➶

reach

19966869

➶

reach

19966869

USP47 binds CTNNB1 and fbxw1. 1 / 1

| 1

➶

sparser

19966869

Fbxw1 activates USP47.

| 2

Fbxw1 activates USP47. 2 / 2

| 2

➶

reach

"However, we can not rule out the possibility that beta-Trcp might target USP47 for proteasome dependent degradation in response to a stimulus that we did not analyze."

19966869

➶

reach

"Depletion of beta-Trcp by siRNA did not induce stabilization of USP47 (XREF_FIG)."

19966869

USP47 affects USP7

| 2 8

USP47 binds USP7.

| 1 8

USP7 binds USP47. 7 / 7

| 1 6

➶

sparser

"Given the association of USP7 and USP47 with the MDM2-p53 pathway xref , xref , we investigated whether the impact of USP7 and USP47 on cancer cell growth is dependent on p53."

37740002

➶

sparser

"Furthermore, activated inflammasomes also regulate USP7 and USP47 activity, which forms a positive feedback pathway for resistance to pathogen infection ( xref )."

34966378

➶

sparser

31679183

➶

sparser

"However, the biggest potential limitation that emerges from this paper is that all of the known USP7 inhibitors (until recently) also interact with USP47 ( Altun et al., 2011 )."

29272698

➶

sparser

38529479

➶

sparser

"Whether USP7 and USP47 directly interact with NLRP3 receptor or with other inflammasome component was not investigated."

31679183

➶

reach

"However, there is no evidence of direct interaction between USP7, USP47, and NLRP3 [XREF_BIBR]."

33138315

USP7 binds USP47 and SHBG. 1 / 1

| 1

➶

sparser

"Strikingly, treatment of LPS‐primed MDMs with nigericin induced a dramatic increase in the proportion of ABP‐bound USP7 and USP47 (Fig xref A)."

30206189

USP7 binds USP47 and NLRP3. 1 / 1

| 1

➶

sparser

"However, there is no evidence of direct interaction between USP7, USP47, and NLRP3 [ xref ]."

33138315

USP47 inhibits USP7.

| 1

USP47 inhibits USP7. 1 / 1

| 1

➶

reach

"In this study we found PTL effectively inhibited USP47 and CRC, which is also an inhibitor of USP7 we previously reported [ 22 ]."

34030041

USP47 affects MAPK

| 8 2

USP47 binds MAPK.

| 3 2

MAPK binds USP47. 5 / 5

| 3 2

➶

reach

33397955

➶

reach

"At the cellular level, interactions between USP47 and MAPK were detected in both overexpression and endogenous experiments (XREF_FIG)."

33489876

➶

reach

"The results of GST pull-down experiment displayed that USP47 directly interacted with MAPK in vitro (XREF_FIG)."

33489876

➶

sparser

"At the cellular level, interactions between USP47 and MAPK were detected in both overexpression and endogenous experiments ( xref )."

33489876

➶

sparser

"The results of GST pull-down experiment displayed that USP47 directly interacted with MAPK in vitro ( xref )."

33489876

USP47 inhibits MAPK.

| 2

USP47 inhibits MAPK. 2 / 2

| 2

➶

3 | 2 4

➶

sparser

"It was identified that YB-1 can interact with USP47 in K562 and primary CML cells (Fig. xref )."

33397955

➶

sparser

"The results show that USP47 interacts with the C-terminal domain (CTD) domain of YB-1 (Fig. xref )."

33397955

➶

reach

33397955

➶

sparser

"USP47 also binds to downstream Y-box binding protein 1 and protects it from proteasome degradation, making it conducive for DNA damage repair to occur in CML cells."

38947742

➶

sparser

33397955

➶

reach

"USP47 also binds to downstream Y-box binding protein 1 and protects it from proteasome degradation, making it conducive for DNA damage repair to occur in CML cells."

38947742

➶

biogrid

No evidence text available

33397955

➶

biogrid

No evidence text available

33397955

➶

biogrid

No evidence text available

33397955

USP47 affects TLE3

2 | 6 1

USP47 deubiquitinates TLE3.

| 4

USP47 deubiquitinates TLE3. 4 / 4

| 4

➶

reach

"USP47 deubiquitylates TLE3 in cells and in vitro."

36749823

➶

reach

"To more directly determine whether USP47 deubiquitylates TLE3, we performed an in vitro ubiquitylation assay with ubiquitylated TLE3 and recombinant USP47 protein."

36749823

➶

reach

"USP47 decreased the amount of ubiquitylated TLE3 in vitro in a dose-dependent manner (Fig. 6B)."

36749823

➶

reach

"To test how deubiquitylation of TLE3 by USP47 alters TLE3 binding to TCF, we performed an in vitro binding assay and found that TLE3 was pulled down with TCF4, but ubiquitylated TLE3 was not (Fig. 6C), consistent with our previous results (7)."

36749823

USP47 binds TLE3.

2 | 1 1

USP47 binds TLE3. 4 / 4

2 | 1 1

➶

biogrid

No evidence text available

36749823

➶

biogrid

No evidence text available

36749823

➶

sparser

"Because our previous studies demonstrated that XIAP ubiquitylates TLE3 ( xref ), we sought to determine whether USP47 interacts with TLE3."

36749823

➶

reach

"We show that although XIAP, USP47, and TLE3 can form dimeric complexes with one another, they are unable to form a stable trimeric complex."

36749823

USP47 decreases the amount of TLE3.

| 1

USP47 decreases the amount of TLE3. 1 / 1

| 1

➶

reach

"USP47 decreased the amount of ubiquitylated TLE3 in vitro in a dose-dependent manner (Fig. 6B)."

36749823

USP47 affects DNA Damage

| 9

USP47 activates DNA Damage. 9 / 9

| 9

➶

reach

"Mechanistic studies have shown that stable Y-box binding protein 1 (YB-1) contributed to USP47-mediated DNA damage repair in CML cells."

34604226

➶

reach

"As a substrate of USP47, YB-1 contributes to USP47 mediated DNA damage repair in CML cells."

33397955

➶

reach

"We further demonstrated that YB-1, a novel substrate of USP47, contributes to USP47 mediated DNA damage repair."

33397955

➶

reach

"Mechanistic studies show that stabilizing Y-box binding protein 1 contributes to USP47 mediated DNA damage repair in CML cells."

33397955

➶

reach

"Taken together, these data demonstrate that by controlling stability of the cytoplasmic form of Pol beta, USP47 modulates the efficiency of DNA damage repair by the BER pathway and maintains genome in[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

21362556

➶

reach

"YB-1 contributes to USP47 mediated DNA damage repair in CML cells."

33397955

➶

reach

"To assess the contribution of YB-1 and Polbeta to USP47 mediated DNA damage repair in CML cells, we overexpressed USP47 in YB-1 and/or POLB knockdown K562 and KBM5 T315I cells."

33397955

➶

reach

"USP47 overexpression in YB-1 and/or Polbeta depletion cells can not abrogate gammaH 2 AX expression, indicating that YB-1 contributes more to USP47 mediated DNA damage repair than Polbeta in CML cells."

33397955

➶

reach

"The results indicate that YB-1 is involved in USP47 mediated DNA damage repair in CML cells."

33397955

P5091 affects USP47

| 2 6

P5091 inhibits USP47. 6 / 6

| 6

➶

reach

"P5091 has been recently reported to also inhibit USP47, one deubiquitinase most closely related to USP7 [27] ."

28216017

➶

reach

"These compounds were found to be highly selective for USP7 in counter-screening against a panel of DUBs including USP10 and USP47, which are also inhibited by P5091."

30486612

➶

reach

"For example, P22077 and P5091 also inhibited USP47 with similar potency [10,77,83,87] ."

34474304

➶

reach

"P5091 is an inhibitor of USP7 and USP47 ( Chauhan et al., 2012; Weinstock et al., 2012 ) and was found to induce apoptosis of multiple myeloma cells resistant to the 20S proteasome inhibitor bortezomi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

25444757

➶

reach

"P5091 has been shown to inhibit tumor growth by inhibiting USP7 and USP47 and is well tolerated in animals [107] ."

29864528

➶

reach

"However, the commercially available inhibitors for these DUBs had no obvious influence on the SOX2 expression, including b-AP15 (the inhibitor of USP14 and UCHL5), P5091 (the inhibitor of USP7 and USP[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

31605775

P5091 inhibits USP47. 2 / 2

| 2

➶

eidos

"Our initial USP7 inhibitor , P5091 , is not specific to USP7 but additionally inhibits USP47 ( ubiquitin-specific peptidase 47 ) , which , among several functions , controls tumor cell proliferation ( 38,39 ) ."

34965932

➶

eidos

"Since the previous studies have demonstrated that P5091 also inhibits Usp47 activity , we chose other specific Usp7 inhibitors ( Usp7-IN-155 and HBX-1981856 ) to validate our results ."

30679505

Valproic acid affects USP47

7 |

Valproic acid decreases the amount of USP47. 7 / 7

7 |

➶

ctd

No evidence text available

28001369

➶

ctd

No evidence text available

26272509

➶

ctd

No evidence text available

25192806

➶

ctd

No evidence text available

24935251

➶

ctd

No evidence text available

24383497

➶

ctd

No evidence text available

23179753

➶

ctd

No evidence text available

27188386

Ubiquitin affects USP47

| 3 4

Ubiquitin binds USP47.

| 1 4

Ubiquitin binds USP47. 5 / 5

| 1 4

➶

sparser

"The structural changes upon Ub-binding to USP47 are similar to those identified for USP7 xref , xref (Fig. xref and Supplementary Fig. xref ), although the changes in the Fingers and BL2 of USP7 are less in magnitude (1.2 and 1.8 Å, respectively)."

37740002

➶

sparser

"The intrinsic flexibility of these loops and the Fingers region is attributed to the interaction between USP47 and ubiquitin."

37740002

➶

sparser

"We employed this assay to conduct a targeted RNAi screen using a focused dsRNA library encompassing factors linked to ubiquitin-proteasome function ( xref ; xref ) that might interact genetically with Usp47 ."

27552662

➶

reach

"Together, Ub binding appears to induce considerable conformational changes in the SL, BL1, BL2, and Fingers, which positions the Ub and realigns the catalytic residues into place for catalysis.The structural changes upon Ub-binding to USP47 are similar to those identified for USP7 (Fig. 3f and Supplementary Fig. 8), although the changes in the Fingers and BL2 of USP7 are less in magnitude (1.2 and 1."

37740002

➶

sparser

"The two c USP47 CD C97S :Ub complexes in an asymmetric unit had an RMSD of 0.54 Å for 354 Cα atoms."

37740002

Ubiquitin inhibits USP47.

| 2

Ubiquitin inhibits USP47. 2 / 2

| 2

➶

reach

"GSK-3beta inactivated by its upstream Akt and ubiquitin-specific protease 47 (USP47) has been shown to inhibit beta-catenin degradation."

36813838

➶

reach

"It has been reported that P22077, a selective ubiquitin-specific protease 7/47 (USP7/USP47) inhibitor, inhibited the proliferation of HIV virus by inhibiting the function of USP47 [27]."

31982568

USP47 affects YTHDF1

| 7

USP47 deubiquitinates YTHDF1. 7 / 7

| 7

➶

reach

"Mechanistically, USP47 prevented YTHDF1 ubiquitination to attenuate the association of YTHDF1 with translation initiation machinery, thereby decreasing m 6 A-based c-Myc translation efficiency."

37788092

➶

reach

"Our immunoblot analyses revealed that overexpression of USP47 with WT ubiquitin or K63-ubiquitin (K63), but not with K48-ubiquitin, inhibited YTHDF1 ubiquitination in HEK293T cells (Figure 6, H and I)."

37788092

➶

reach

"In contrast, catalytically inactive USP47 did not deubiquitinate YTHDF1 in HEK293T cells (Figure 6I)."

37788092

➶

reach

"These data indicate that USP47 prevents YTHDF1 ubiquitination and then attenuates the association of YTHDF1 with the translation initiation machinery in the Tregs."

37788092

➶

reach

"The present study revealed that USP47 prevented the ubiquitination of YTHDF1 and thus prevented its associations with eIF3A to suppress m A-based c-Myc translation in Tregs."

37788092

➶

reach

"Furthermore, deubiquitinase ubiquitin-specific peptidase 47 (USP47) was found to prevent YTHDF1 ubiquitination, thereby disrupting its interaction with the translation initiation machinery and reducing m6A-mediated c-MYC translation efficiency, which is crucial for maintaining Treg cell metabolic and functional homeostasis [80]."

39987091

➶

reach

"USP47 can prevent the ubiquitination of YTHDF1 and reduce its ability to promote translation, thereby exerting control over c-Myc expression."

38202722

USP47 affects PNLIP

| 3 4

USP47 binds PNLIP. 7 / 7

| 3 4

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34030041

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reach

"To assess whether PTL binds to USP47, CETSA was firstly undertaken in HCT116 cell lysates."

34030041

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reach

34030041

➶

sparser

34030041

➶

sparser

"To assess whether PTL binds to USP47, CETSA was firstly undertaken in HCT116 cell lysates."

34030041

➶

sparser

"Next, in order to investigate whether PTL interacts with USP47 in intact cells, HCT116 cells treated with PTL or DMSO were collected, lysed and heated."

34030041

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sparser

34030041

USP47 affects MYC

| 7

USP47 inhibits MYC.

| 6

USP47 inhibits MYC. 6 / 6

| 6

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reach

"Therefore, USP47 acts in a YTHDF1-dependent manner to inhibit m A-dependent c-Myc translation and maintain Treg metabolism and homeostasis."

37788092

➶

reach

"Here, we identified that the deubiquitinase USP47 inhibited c-Myc translation mediated by the RNA N 6 -methyladenosine (m 6 A) reader YTHDF1 to maintain Treg metabolic and functional homeostasis."

37788092

➶

reach

"USP47 deficiency in Tregs triggered the accumulation of the c-Myc protein and in turn exacerbated hyperglycolysis."

37788092

➶

reach

"RNA sequencing and biochemical and metabolic analysis demonstrated that USP47 deficiency promoted c-Myc protein accumulation and hyperglycolysis, which contributed to defective Treg homeostasis."

37788092

➶

reach

"Therefore, USP47 inhibits excessive c-Myc activity and hyperglycolysis in Tregs."

37788092

➶

reach

"USP47 inhibits m 6 A-dependent c-Myc translation to maintain regulatory T cell metabolic and functional homeostasis."

37788092

USP47 activates MYC.

| 1

USP47 activates MYC. 1 / 1

| 1

➶

reach

"In this study, we show that USP47 directs YTHDF1-dependent c-Myc translation to maintain Treg metabolic and functional homeostasis."

37788092

CTNNB1 affects USP47

| 3 4

CTNNB1 binds USP47.

| 3 3

USP47 binds CTNNB1. 5 / 5

| 3 2

➶

reach

33397955

➶

sparser

"USP47 directly interacts with β-catenin and affects Wnt/β-catenin signaling by deubiquitination of β-catenin."

32486158

➶

reach

"Both USP15 and USP47 form a complex with beta-catenin leading to its deubiquitination and stabilization [248,249]."

28923280

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sparser

"The interaction between β-catenin and USP47 protein was weakened with the loss of FAM83H and increased with overexpression of FAM83H ( xref )."

32564009

➶

reach

"USP47 directly interacts with beta-catenin and affects Wnt and beta-catenin signaling by deubiquitination of beta-catenin."

32486158

USP47 binds CTNNB1 and fbxw1. 1 / 1

| 1

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sparser

19966869

CTNNB1 ubiquitinates USP47.

| 1

CTNNB1 ubiquitinates USP47. 1 / 1

| 1

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sparser

"As a deubiquitinase, USP47 prevents β-catenin ubiquitination. knockdown of USP47 boosts β-catenin ubiquitination, reduces Wnt signaling, and inhibits the development of cancer cells [26] ."

37001379

Bisphenol A affects USP47

6 |

Bisphenol A increases the amount of USP47.

4 |

Bisphenol A increases the amount of USP47. 4 / 4

4 |

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No evidence text available

30816183

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ctd

No evidence text available

25181051

➶

ctd

No evidence text available

26063408

➶

ctd

No evidence text available

32528016

Bisphenol A decreases the amount of USP47.

2 |

Bisphenol A decreases the amount of USP47. 2 / 2

2 |

➶

ctd

No evidence text available

31675489

➶

ctd

No evidence text available

33243503

XIAP affects USP47

2 | 2 2

USP47 binds XIAP. 6 / 6

2 | 2 2

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reach

"We found that TLE3 inhibited the interaction between USP47 and XIAP, suggesting that TLE3 and USP47 compete for binding to XIAP and that the three proteins do not form a heterotrimeric complex (Fig. 4D, S7A)."

36749823

➶

reach

"We show that although XIAP, USP47, and TLE3 can form dimeric complexes with one another, they are unable to form a stable trimeric complex."

36749823

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sparser

36749823

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biogrid

No evidence text available

36749823

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biogrid

No evidence text available

36749823

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sparser

"Furthermore, we cannot distinguish whether XIAP and USP47 have overlapping binding sites on TLE3 or whether the binding of XIAP or USP47 induces a conformational change in TLE3 that precludes the binding of USP47 or XIAP, respectively."

36749823

USP47 affects liver regeneration

| 6

USP47 activates liver regeneration. 6 / 6

| 6

➶

reach

"Similar strategies could be employed to uncover USP47 substrates in hepatocytes in the context of liver regeneration to provide more mechanistic insights for this process.Another caveat in our proposa[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

37487274

➶

reach

"At this point, the mechanism whereby USP47 contributes to liver regeneration is not clear."

37487274

➶

reach

"Because inflammasome-mediated processing of IL-1β and IL-18 has been shown to be indispensable for liver regeneration in a model of acute liver failure [ 57 ], it is possible that USP47 may contribute[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

37487274

➶

reach

"Therefore, we hypothesized that USP47 might contribute to liver regeneration by regulating proliferation of hepatocytes."

37487274

➶

reach

"USP47 deletion or inhibition dampens liver regeneration whereas USP47 over-expression enhances liver regeneration in mice.All the animal experiments were reviewed and approved by the Nanjing Medical U[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

37487274

➶

reach

"Despite these observations, our finding adds to the growing list of genes targeted by E2F1 during liver regeneration.Second, we show that USP47 deletion or inhibition attenuates whereas USP47 over-exp[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

37487274

USP47 affects XIAP

2 | 2 2

USP47 binds XIAP. 6 / 6

2 | 2 2

➶

reach

36749823

➶

reach

"We show that although XIAP, USP47, and TLE3 can form dimeric complexes with one another, they are unable to form a stable trimeric complex."

36749823

➶

sparser

36749823

➶

biogrid

No evidence text available

36749823

➶

biogrid

No evidence text available

36749823

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sparser

36749823

USP47 affects TCEA3

2 | 2 2

USP47 binds TCEA3.

2 | 1 2

TCEA3 binds USP47. 5 / 5

2 | 1 2

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sparser

"These results indicated that the USP47-TCEA3 axis modulates cell pyroptosis and apoptosis and may serve as a target for therapeutic intervention in CRC."

34630087

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sparser

"USP47 binds to TCEA3 and regulates its deubiquitination and intracellular levels, thereby enhancing doxorubicin resistance in CRC cells. [ xref ] STAT3β treatment enhanced cisplatin sensitivity and GSDME‐dependent pyroptosis in ESCC cells; mechanistic tests showed that STAT3β treatment increased ROS levels in cisplatin‐treated cells, thereby activating caspase‐3 and GSDME and thus inducing pyroptosis."

37436087

➶

biogrid

No evidence text available

34630087

➶

biogrid

No evidence text available

34630087

➶

reach

"USP47 binds to TCEA3 and regulates its deubiquitination and intracellular levels, thereby enhancing doxorubicin resistance in CRC cells."

37436087

USP47 activates TCEA3.

| 1

USP47 activates TCEA3. 1 / 1

| 1

➶

reach

"While ectopic expression of USP47 increased cellular TCEA3 and resistance to doxorubicin, the effect was markedly attenuated by TCEA3 knockdown."

34630087

USP47 affects RPL11

2 | 4

USP47 binds RPL11. 6 / 6

2 | 4

➶

biogrid

No evidence text available

35205710

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biogrid

No evidence text available

35205710

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sparser

"To solve this question, we will investigate whether this lncRNA affects the interaction of USP47 with RPL11 or the deubiquitination and translocation of RPL11 by USP47, and thus is involved in the p53 protein levels as in our models."

35205710

➶

sparser

"To confirm this possibility, we first investigated the interaction between USP47 and RPL11."

35205710

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sparser

"When we co-transfected with Flag-tagged USP47 and HA-tagged RPL11 in the cells, and we observed the binding of USP47 to RPL11 by immunoprecipitation with anti-Flag magnetic beads ( xref a)."

35205710

➶

sparser

"In addition, USP47 binds to and deubiquitinates RPL11, and USP47-induced RPL11 deubiquitination is inhibited by miR-101-3p."

35205710

USP47 affects Neoplasm Invasiveness

| 6

USP47 activates Neoplasm Invasiveness. 6 / 6

| 6

➶

reach

"The overexpression of USP47 or SATB1 alone or together promoted cell invasion in HCT116 ( Fig. 3 O and S5D ) and DLD1 ( Figs."

30742943

➶

reach

"In addition, the USP47 knockdown attenuated the invasiveness of DLD-1 cells."

29162839

➶

reach

"Overexpression of USP47 enhances invasiveness and metastatic potential of CRC cells."

29162839

➶

reach

37564825

➶

reach

"USP47 promotes colon cancer cell proliferation, tumorigenesis, and invasion partially through SATB1."

30742943

➶

reach

"In addition, we demonstrated that USP47 promotes colon cancer cell metastasis and invasion by upregulating SATB1 expression.Over the past decade, increasing studies have reported that SATB1 is highly [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

30742943

RPL11 affects USP47

2 | 4

USP47 binds RPL11. 6 / 6

2 | 4

➶

biogrid

No evidence text available

35205710

➶

biogrid

No evidence text available

35205710

➶

sparser

35205710

➶

sparser

"To confirm this possibility, we first investigated the interaction between USP47 and RPL11."

35205710

➶

sparser

"When we co-transfected with Flag-tagged USP47 and HA-tagged RPL11 in the cells, and we observed the binding of USP47 to RPL11 by immunoprecipitation with anti-Flag magnetic beads ( xref a)."

35205710

➶

sparser

"In addition, USP47 binds to and deubiquitinates RPL11, and USP47-induced RPL11 deubiquitination is inhibited by miR-101-3p."

35205710

USP47 is modified

| 1 5

USP47 is ubiquitinated.

| 1 4

USP47 is ubiquitinated. 5 / 5

| 1 4

➶

sparser

"Furthermore, USP47 deubiquitinates itself, whereas β-TrCP promotes USP47 ubiquitination through interaction with an atypical motif in USP47."

26169834

➶

trips

"Furthermore, USP47 deubiquitinates itself, whereas β-TrCP promotes USP47 ubiquitination through interaction with an atypical motif in USP47."

26169834

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sparser

"Finally, attempts to ubiquitylate USP47 in the presence of purified SCF β-Trcp , E1 and several E2s (UbcH5 and UbcH3) had negative results (data not shown)."

19966869

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sparser

"This statement is based on the following evidences: (i) USP47 is a stable protein, with a half-life of over 8 h; (ii) proteasome activity does not regulate USP47 protein levels; (iii) silencing of β-Trcp does not induce stabilization of USP47; (iv) USP47 expression is constant during a variety of processes, such as cell-cycle progression, DNA damage checkpoint responses or TNF signaling; and (v) SCF β-Trcp is not able to mediate USP47 ubiquitylation in vitro ."

19966869

➶

sparser

"Interestingly, β-TrCP directly binds to the USP47 motif at DSGXXXS and regulates USP47 ubiquitination [ xref ]."

37686524

USP47 is phosphorylated.

| 1

USP47 is phosphorylated. 1 / 1

| 1

➶

sparser

"CYLD phosphorylation serine (S)418 and S422 sites are well documented in independent studies ( xref , xref ) and shown in xref C . USP34 phosphorylation S718 and S1751 sites have been identified in HeLa cells ( xref ) and are not shown in xref D , since there were less than ten reports, whereas no additional USP47 phosphorylation sites have been identified."

37865315

USP47 affects Ubiquitin

| 1 4

Ubiquitin binds USP47. 5 / 5

| 1 4

➶

sparser

37740002

➶

sparser

"The intrinsic flexibility of these loops and the Fingers region is attributed to the interaction between USP47 and ubiquitin."

37740002

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sparser

27552662

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reach

37740002

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sparser

"The two c USP47 CD C97S :Ub complexes in an asymmetric unit had an RMSD of 0.54 Å for 354 Cα atoms."

37740002

USP47 affects USP47

| 1 4

USP47 increases the amount of USP47.

| 3

USP47 increases the amount of USP47. 3 / 3

| 3

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reach

"High phosphorus treatment significantly increased the expression of USP47, RUNX2, and RANKL in RASMCs, while USP47 siRNA transfection significantly reduced the expression of USP47, RUNX2, and RANKL in RASMCs."

35509185

➶

reach

"Real-time PCR results demonstrated that USP47 siRNA transfection effectively reduced the mRNA expression of USP47 in RASMCs."

| 4

USP47 inhibits SMURF2. 4 / 4

| 4

➶

reach

"SMURF2 is negatively regulated by USP47, and USP47 depletion sensitizes colon cancer cells to 5-FU-treatment-induced apoptosis."

30742943

➶

reach

"Research has shown that ubiquitin-specific peptidase (USP47) negatively regulates Smurf2 to mediate colorectal cancer cell proliferation and tumor progression, but how USP47 regulates Smurf2 expressio[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

37307883

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reach

"Furthermore, we show that SMURF2 functions as the E3 ubiquitin ligase for SATB1 and is negatively regulated by USP47."

30742943

➶

reach

"Likewise, we reported that SMURF2 was negatively regulated by USP47 at the mRNA and protein levels."

30742943

USP47 binds SMURF2.

| 1

USP47 binds SMURF2. 1 / 1

| 1

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reach

"USP47 interacted with SATB1 through its catalytic core domain and antagonized Smurf2-mediated SATB1 degradation."

34604226

USP47 affects POLB

1 1 | 3

USP47 deubiquitinates POLB.

1 | 3

USP47 deubiquitinates POLB. 3 / 3

1 | 2

➶

reach

"USP47 may also be an oncology target, since it deubiquitinates DNA polymerase β (POLB) to regulate DNA base excision repair (BER) (Parsons et al. 2011)."

36302555

➶

reach

"Specifically, USP47 deubiquitinates and stabilizes MAPK 25, DNA polymerase beta (Polbeta) 26, E-cadherin 27, beta-catenin 28, SNAIL 29, YAP 30, beta-Trcp 31, and katanin-p60 32."

33397955

➶

ubibrowser

sparser

"Next, to examine whether USP47 directly binds to IK, cell lysates expressing HA-IK and Flag-USP47 were used in a pull-down assay with anti-HA or -Flag antibodies."

32377397

➶

sparser

"Moreover, ectopically overexpressed Flag-USP47 interacted with endogenous RPS2 ( xref )."

32370049

➶

sparser

"In contrast, the overexpression of Flag-USP47 decreased the p53 level, and it was also rescued by treatment with MG132 ( xref b)."

32370049

➶

sparser

"To verify the interaction of USP47 and RPS2, we performed immunoprecipitation with anti-Flag agarose after overexpressing HA-RPS2 and Flag-USP47."

32370049

USP47 affects Carcinogenesis

| 1 4

USP47 activates Carcinogenesis. 5 / 5

| 1 4

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reach

37924851

➶

reach

"USP47 stimulates cell growth, tumorigenesis and migration through SATB1."

30742943

➶

reach

"USP47 deficiency impairs transcriptional activity of SATB1 target genes and inhibits proliferation, migration, and tumorigenesis (68, 69)."

40071088

➶

eidos

"USP47 Promotes Tumorigenesis by Negative Regulation of p53 through Deubiquitinating Ribosomal Protein S2 p53 is activated in response to cellular stresses such as DNA damage , oxidative stress , and especially ribosomal stress ."

32370049

➶

reach

"USP47 promotes colon cancer cell proliferation, tumorigenesis, and invasion partially through SATB1."

30742943

USP47 affects BCR-ABL

| 5

USP47 activates BCR-ABL. 4 / 4

| 4

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reach

"We show that knockout of Usp47 significantly prevents BCR-ABL or BCR-ABL T315I -induced leukemogenesis and reduces the number of GFP + LSK cells in CML mice, indicating that Usp47 is critical in the development of BCR-ABL-induced CML."

33397955

➶

reach

"USP47 knockdown significantly inhibited both BCR-ABL-induced CML in mice and BCR-ABL -induced CML, which are known to confer Imatinib and second-generation TKI resistance ."

37740002

➶

reach

"USP47 knockout significantly inhibited BCR-ABL and BCR-ABL -induced mouse CML with reduced of LIN Sca1 c-kit CML stem cells/progenitor cells."

34604226

➶

reach

"The knockout of Usp47 significantly inhibits BCR-ABL and BCR-ABL T315I -induced CML in mice with the reduction of Lin - Sca1 + c-Kit + CML stem and progenitor cells."

33397955

USP47 activates BCR-ABL-T315I. 1 / 1

| 1

➶

reach

33397955

TCEA3 affects USP47

2 | 1 2

TCEA3 binds USP47. 5 / 5

2 | 1 2

➶

sparser

"These results indicated that the USP47-TCEA3 axis modulates cell pyroptosis and apoptosis and may serve as a target for therapeutic intervention in CRC."

34630087

➶

sparser

37436087

➶

biogrid

No evidence text available

34630087

➶

biogrid

No evidence text available

34630087

➶

reach

"USP47 binds to TCEA3 and regulates its deubiquitination and intracellular levels, thereby enhancing doxorubicin resistance in CRC cells."

37436087

MAPK affects USP47

| 3 2

MAPK binds USP47. 5 / 5

| 3 2

➶

reach

33397955

➶

reach

"At the cellular level, interactions between USP47 and MAPK were detected in both overexpression and endogenous experiments (XREF_FIG)."

33489876

➶

reach

"The results of GST pull-down experiment displayed that USP47 directly interacted with MAPK in vitro (XREF_FIG)."

33489876

➶

sparser

"At the cellular level, interactions between USP47 and MAPK were detected in both overexpression and endogenous experiments ( xref )."

33489876

➶

sparser

"The results of GST pull-down experiment displayed that USP47 directly interacted with MAPK in vitro ( xref )."

33489876

KCNQ1OT1 affects USP47

| 2 3

KCNQ1OT1 increases the amount of USP47.

| 2

KCNQ1OT1 increases the amount of USP47. 2 / 2

| 2

➶

| 5

➶

sparser

"In addition, cell lysates obtained without the overexpression of HA-IK and Flag-USP47 were immunoprecipitated with the anti-USP47 monoclonal antibody."

32377397

➶

sparser

"Next, to examine whether USP47 directly binds to IK, cell lysates expressing HA-IK and Flag-USP47 were used in a pull-down assay with anti-HA or -Flag antibodies."

32377397

➶

sparser

"Moreover, ectopically overexpressed Flag-USP47 interacted with endogenous RPS2 ( xref )."

32370049

➶

sparser

"In contrast, the overexpression of Flag-USP47 decreased the p53 level, and it was also rescued by treatment with MG132 ( xref b)."

32370049

➶

sparser

"To verify the interaction of USP47 and RPS2, we performed immunoprecipitation with anti-Flag agarose after overexpressing HA-RPS2 and Flag-USP47."

32370049

MiR-204-5p affects USP47

| 4

MiR-204-5p inhibits USP47. 4 / 4

| 4

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reach

"Another study revealed that miR-204-5p inhibited GC cell proliferation via downregulation of USP47 and RAB22A [31]."

36157241

➶

reach

"Furthermore, another study indicated that miR-204-5p suppressed gastric cancer cell growth by inhibiting RAB22A and USP47 [42]."

33888645

➶

reach

"USP47 is inhibited by miR-204-5p, which represses GC cell proliferation [65]."

36497313

➶

reach

"Additionally, it was determined that miR-204-5p was downregulated in GC, and may inhibit the proliferation of GC cells by targeting USP47 and RAB22A, thus serving a role in suppressing cancer development."

31897112

USP47 affects cell growth

| 4

USP47 inhibits cell growth.

| 2

USP47 inhibits cell growth. 2 / 2

| 2

➶

reach

"As the s, P5091 is also an elective dual-inhibitor of USP7 and USP47 that can suppress CRC cell growth and induces their apoptosis in vitro (An et al., 2017)."

37251574

➶

reach

"Silencing of USP47 induced a cell growth defect that appeared on day 6 and was sustained through day 9 (XREF_FIG), indicating that depletion of USP47 inhibits cell growth, likely by inducing apoptosis."

19966869

USP47 activates cell growth.

| 2

USP47 activates cell growth. 2 / 2

| 2

➶

reach

"USP47 stimulates cell growth, tumorigenesis and migration through SATB1."

30742943

➶

reach

19966869

USP47 affects TRAF6

3 | 1

TRAF6 binds USP47. 3 / 3

3 |

➶

biogrid

No evidence text available

35235149

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biogrid

No evidence text available

37001379

➶

biogrid

No evidence text available

37001379

USP47 binds TRAF6 and 6 A. 1 / 1

| 1

➶

reach

"The interaction between USP47 and TRAF3 or TRAF6 was confirmed ( Fig. 6 A, B, D)."

37001379

USP47 affects Pol beta

| 4

USP47 increases the amount of Pol beta.

| 2

USP47 increases the amount of Pol beta. 2 / 2

| 2

➶

reach

"Collectively, these studies highlight the discovery of an important pathway involved in the regulation of both the cellular steady state level, and the levels in response to DNA damage, of Pol beta wh[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

| 4

➶

reach

"USP47 subsequently stabilizes Snail by deubiquitination and promotes EMT and tumor metastasis (35)."

35616129

➶

reach

"USP47 knockdown dramatically reduced metastasis, as evidenced by lower bioluminescence intensity (Supplemental Figure 6, G and H)."

39998882

➶

reach

"Taken together, our results reveal an oncogenic function of USP47 in promoting GC cell proliferation and metastasis, both in vitro and in vivo."

39998882

➶

reach

30742943

USP47 affects NNT

| 3 1

USP47 binds NNT.

| 2 1

USP47 binds NNT. 3 / 3

| 2 1

➶

reach

"Then we explored the direct interaction between USP47 and NNT through co-immunoprecipitation (IP) analysis and the results revealed that USP47 -knockdown by lentivirus infection induced increased ubiq[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

37924851

➶

reach

"These questions warrant further study in the future.Taken together, the present study showed that the deubiquitinating enzyme USP47 interacts with and stabilizes NNT by antagonizing its ubiquitination[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

37924851

➶

sparser

37924851

USP47 activates NNT.

| 1

USP47 activates NNT. 1 / 1

| 1

➶

reach

"Altogether, these results highlighted NNT as the direct target of USP47 in irradiated skin cells, which is involved in radiation-induced skin injury.In addition, to understand the role of NNT in the p[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

37924851

USP47 affects IK

2 | 2

IK binds USP47. 4 / 4

2 | 2

➶

biogrid

No evidence text available

32377397

➶

biogrid

No evidence text available

32377397

➶

sparser

"In summary, we found that IK depletion impairs the proper splicing of ATM pre-mRNA at exons 1–2, which results in ATM deficiency followed by chromosome fragmentation and that USP47 directly binds to IK and deubiquitinates it leading to its stabilization."

32377397

➶

sparser

"Next, to examine whether USP47 directly binds to IK, cell lysates expressing HA-IK and Flag-USP47 were used in a pull-down assay with anti-HA or -Flag antibodies."

32377397

USP47 affects ERAP1

| 2 2

ERAP1 binds USP47. 4 / 4

| 2 2

➶

sparser

"The interaction of the positive HH regulator ERAP1 with USP47 induces the degradation of βTrCP, thus protecting GLIs from βTrCP-dependent degradation and stimulating HH activity [ xref ]."

32531973

➶

reach

"We demonstrate that ERAP1 binds the deubiquitylase enzyme USP47, displaces the USP47 associated betaTrCP, the substrate-receptor subunit of the SCF betaTrCP ubiquitin ligase, and promotes betaTrCP degradation."

31341163

➶

sparser

"ERAP1-USP47 binding disrupts the USP47-β-TrCP binding and subsequent ubiquitination and proteasomal degradation of β-TrCP, leading to increases in the levels of the GLI1 and GLI2 proteins in the Hedgehog (Hh) pathway."

37686524

➶

reach

"Bufalieri et al. identified endoplasmic reticulum aminopeptidase 1 (ERAP1) as a new player in the Hedgehog pathway, and ERAP1 binds to USP47, displacing β-TrCP from SCF , a ubiquitin ligase, therefore promoting β-TrCP degradation ."

37740002

USP47 affects BCR

| 4

USP47 activates BCR. 4 / 4

| 4

➶

reach

"The knockout of USP47 or using P22077 also significantly impaired BCR-ABL and BCR-ABL -induced CML in mice and reduced the number of CML stem/progenitor cells."

38947742

➶

reach

"USP47 knockdown significantly inhibited both BCR-ABL-induced CML in mice and BCR-ABL -induced CML, which are known to confer Imatinib and second-generation TKI resistance ."

37740002

➶

reach

"In IM-resistant CML, pharmacological inhibition of ubiquitin-specific peptidase 47 (USP47) with P22077 reduces the percentage of CD34 CD38 cells in secondary BM transplantation and inhibits colony-forming activity of CD34 cells from IM-resistant CML patients while sparing normal CD34 cells [234]."

36009388

➶

reach

"USP47 knockout significantly inhibited BCR-ABL and BCR-ABL -induced mouse CML with reduced of LIN Sca1 c-kit CML stem cells/progenitor cells."

34604226

USP47 affects BACH1

1 | 3

USP47 deubiquitinates BACH1.

| 3

USP47 deubiquitinates BACH1. 3 / 3

| 3

➶

reach

"Furthermore, the induction of BACH1 deubiquitination by USP47 promotes the Warburg effect and progression in non-small cell lung cancer, as demonstrated by metabolism-related indicators [67]."

38321558

➶

reach

"BACH1 stability was mediated by USP47 via enhancing the deubiquitylation of BACH1 [36]."

38195593

➶

reach

"USP47 could deubiquitinate BACH1, glyceraldehyde-phosphate dehydrogenase (GAPDH), and hexokinase 2 (HK2), to promote NSCLC progression (73)."

37795365

USP47 binds BACH1.

| 1

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"The interaction between USP47 and TRAF3 or TRAF6 was confirmed ( Fig. 6 A, B, D)."

37001379

TLE3 affects USP47

2 | 1 1

USP47 binds TLE3. 4 / 4

2 | 1 1

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biogrid

No evidence text available

36749823

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biogrid

No evidence text available

36749823

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sparser

"Because our previous studies demonstrated that XIAP ubiquitylates TLE3 ( xref ), we sought to determine whether USP47 interacts with TLE3."

36749823

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reach

"We show that although XIAP, USP47, and TLE3 can form dimeric complexes with one another, they are unable to form a stable trimeric complex."

36749823

MYCBP2 affects USP47

| 4

MYCBP2 increases the amount of USP47.

| 3

MYCBP2 increases the amount of USP47. 3 / 3

| 3

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"Thus, we concluded that PAM induces USP47 expression, which subsequently deubiquitinated β-catenin, leading to β-catenin stabilization in hDPCs."

36813838

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reach

"Similarly, PAM significantly induced FER and USP47 expression, compared to GAM (Fig. 3A)."

36813838

➶

reach

"The results revealed that PAM significantly increased USP47 expression and decreased β-catenin phosphorylation (Ser33/37/Thr41) at 15 min and 1 h after treatment (Fig. 3B and C)."

36813838

MYCBP2 decreases the amount of USP47.

| 1

MYCBP2 decreases the amount of USP47. 1 / 1

| 1

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"PAM treatment also induced beta-catenin translocation and inhibited beta-catenin ubiquitination by activating Akt/GSK-3beta signaling and upregulating USP47 expression."

36813838

IK affects USP47

2 | 2

IK binds USP47. 4 / 4

2 | 2

➶

biogrid

No evidence text available

32377397

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biogrid

No evidence text available

32377397

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sparser

32377397

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sparser

"Next, to examine whether USP47 directly binds to IK, cell lysates expressing HA-IK and Flag-USP47 were used in a pull-down assay with anti-HA or -Flag antibodies."

32377397

ERAP1 affects USP47

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ERAP1 binds USP47. 4 / 4

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"The interaction of the positive HH regulator ERAP1 with USP47 induces the degradation of βTrCP, thus protecting GLIs from βTrCP-dependent degradation and stimulating HH activity [ xref ]."

32531973

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31341163

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37686524

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37740002

E2F7 affects USP47

| 2 2

E2F7 activates USP47.

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E2F7 activates USP47. 3 / 3

| 2 1

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eidos

"E2F7 upregulated USP47 expression by downregulating miR-199b in SW403 cells ."

33489876

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"Therefore, we are convinced that present results show that E2F7 suppressed the miR-199b expression and promoted USP47 to stabilize MAPK proteins, thereby contributing to colon cancer development."

33489876

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eidos

"USP47 upregulation by E2F7 deubiquitin-modifies MAPK K48 ubiquitin chain to stabilize MAPK ."

33489876

E2F7 increases the amount of USP47.

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E2F7 increases the amount of USP47. 1 / 1

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"E2F7 upregulated USP47 expression by downregulating miR-199b in SW403 cells."

33489876

E2F1 affects USP47

| 4

E2F1 increases the amount of USP47.

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E2F1 increases the amount of USP47. 3 / 3

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"Our data show that USP47 expression is up-regulated in hepatocytes at the transcriptional level by E2F1 during liver regeneration."

37487274

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"Silencing E2F1 expression with two different pairs of siRNAs equally attenuated USP47 expression at both mRNA levels ( Fig. 2 E) and protein levels ( Fig. 2 F)."

37487274

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"First, USP47 expression is up-regulated, at the transcriptional level by E2F1, during liver regeneration."

37487274

E2F1 activates USP47.

| 1

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33397955

MiR-454 affects USP47

| 3

MiR-454 activates USP47.

| 2

MiR-454 activates USP47. 2 / 2

| 2

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"Furthermore, the results indicated that miR-454 directly targeted USP47."

33576460

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"USP47 is directly targeted by miR-454."

33576460

MiR-454 binds USP47.

reach

"And the overexpression of USP47 promoted the glycolysis capacity of lung cancer cells."

35141006

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37564825

USP47 affects UBXN1

3 |

UBXN1 binds USP47. 3 / 3

3 |

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biogrid

No evidence text available

33961781

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biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

26186194

USP47 affects UBC

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UBC binds USP47. 3 / 3

3 |

➶

biogrid

No evidence text available

33141564

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biogrid

No evidence text available

29576527

➶

biogrid

No evidence text available

37740002

USP47 affects STN1

3 |

STN1 binds USP47. 3 / 3

3 |

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biogrid

No evidence text available

33961781

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biogrid

No evidence text available

28514442

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biogrid

No evidence text available

26186194

USP47 affects RPS2-mediated MDM2

| 3

USP47 inhibits RPS2-mediated MDM2. 3 / 3

| 3

➶

eidos

"Taken together , these results suggest that USP47 inhibits the interaction between RPS2 and MDM2 , thereby decreasing RPS2-mediated inhibition of MDM2 under normal conditions ( Figure 2 ; Figure 3 ) ."

32370049

➶

eidos

"USP47 inhibits the interaction between RPS2 and MDM2 thereby alleviating RPS2-mediated suppression of MDM2 under normal conditions ."

32370049

➶

eidos

"USP47 deubiquitinates RPS2 and alleviates RPS2-mediated inhibition of MDM2 ."

32370049

USP47 affects POLR2M

3 |

POLR2M binds USP47. 3 / 3

3 |

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biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

33961781

➶

biogrid

No evidence text available

26186194

USP47 affects Mice

| 3

USP47 activates Mice. 3 / 3

| 3

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reach

"Moreover, USP47 and EBAG9 protein expressions were elevated in renal I/R injury mice."

37083129

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reach

37487274

➶

reach

"USP47 knockdown significantly inhibited both BCR-ABL-induced CML in mice and BCR-ABL -induced CML, which are known to confer Imatinib and second-generation TKI resistance ."

37740002

USP47 affects MAPK14

3 |

MAPK14 binds USP47. 3 / 3

3 |

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biogrid

No evidence text available

33961781

➶

biogrid

No evidence text available

26186194

➶

biogrid

No evidence text available

28514442

USP47 affects HOGA1

3 |

HOGA1 binds USP47. 3 / 3

3 |

➶

biogrid

No evidence text available

33961781

➶

biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

26186194

USP47 affects HK2

| 3

USP47 deubiquitinates HK2.

| 2

USP47 deubiquitinates HK2. 2 / 2

| 2

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reach

"USP47 could deubiquitinate BACH1, glyceraldehyde-phosphate dehydrogenase (GAPDH), and hexokinase 2 (HK2), to promote NSCLC progression (73)."

37795365

➶

reach

"USP47 could deubiquitinate BACH1, glyceraldehyde-phosphate dehydrogenase (GAPDH), and hexokinase 2 (HK2), to promote NSCLC progression (73)."

37795365

USP47 activates HK2.

| 2 1

PRMT5 binds USP47.

| 1 1

USP47 binds PRMT5. 2 / 2

| 1 1

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"Mechanistically, USP47 bound, deubiquitinated, and stabilized protein arginine methyltransferase 5 (PRMT5), thus upregulating OGA expression."

39436323

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reach

"Mechanistically, USP47 bound, deubiquitinated, and stabilized protein arginine methyltransferase 5 (PRMT5), thus upregulating OGA expression."

39436323

PRMT5 activates USP47.

37924851

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sparser

37924851

MAPK14 affects USP47

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MAPK14 binds USP47. 3 / 3

3 |

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biogrid

No evidence text available

33961781

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biogrid

No evidence text available

26186194

➶

biogrid

No evidence text available

28514442

LINC00668 affects USP47

| 3

LINC00668 inhibits USP47.

| 2

LINC00668 inhibits USP47. 2 / 2

| 2

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reach

"The rescue experiments for luciferase reporter assay and functional assays indicated that overexpression of LINC00668 apparently attenuated the inhibition of USP47 caused by miR-188–5p, suggesting tha[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

31233752

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"We found that USP47 expression was apparently downregulated by sh-LINC00668 and elevated by miR-188–5p depletion compared with negative control groups, and the reduction in USP47 caused by LINC00668 k[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

31233752

LINC00668 increases the amount of USP47.

biogrid

No evidence text available

➶

| 1

USP47 inhibits imatinib. 1 / 1

| 1

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reach

"Downregulation of USP47 inhibited the proliferation of imatinib-sensitive or drug-resistant CML cells."

34604226

USP47 activates imatinib.

| 1

USP47 activates imatinib. 1 / 1

| 1

➶

eidos

"By this mechanism , depletion of USP47 inhibits cancer cell growth and colony formation in a p53-dependent manner and it was shown to attenuate tumorigenesis in a xenograft mouse model ."

32370049

USP47 affects cell death

| 2

USP47 activates cell death. 2 / 2

| 2

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"As shown in XREF_FIG, the genetic depletion of USP47 in mouse embryonic fibroblasts increased the sensitivity to the ultraviolet induced cell death."

19966869

➶

reach

"The pro apoptotic effect elicited by the silencing of USP47 prompted us to analyze whether depletion of USP47 could increase cell death triggered by anticancer drugs in different tumor cell lines."

19966869

USP47 affects cancer cell growth

reach

"Compared with the shNC group, USP47 knockdown significantly suppressed NPC cell viability and DDP resistance, which was significantly reversed by co-transfection with miR-454 inhibitor."

33576460

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reach

"In the present study, USP47 was identified as a target of miR-454, and USP47 knockdown significantly inhibited cell viability and DDP resistance in NPC cells compared with the shNC group."

33576460

USP47 affects TBK1

| 2

USP47 phosphorylates TBK1.

| 1

USP47 leads to the phosphorylation of TBK1. 1 / 1

| 1

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reach

"As shown in Fig. 5 B, overexpression of USP47 inhibited SeV-induced phosphorylation of TBK1 and IRF3."

37001379

USP47 dephosphorylates TBK1.

reach

39048945

USP47 activates Leukemia.

➶

reach

"Furthermore, we show that SMURF2 functions as the E3 ubiquitin ligase for SATB1 and is negatively regulated by USP47."

30742943

USP47 binds E3_Ub_ligase.

32370049

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sparser

32370049

FGF2 affects USP47

biogrid

No evidence text available

29395067

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| 1

USP47 binds β-TrCP1/2. 1 / 1

| 1

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reach

"USP47 protein levels are little changed, although USP47 binds specifically to β-TrCP1/2 at the WD-repeat domain [143]."

37686524

Tremolite asbestos affects USP47

1 |

Tremolite asbestos decreases the amount of USP47. 1 / 1

1 |

1 |

➶

ctd

No evidence text available

39786591

Sodium arsenate affects USP47

1 |

Sodium arsenate increases the amount of USP47. 1 / 1

1 |

➶

ctd

No evidence text available

21795629

ShUSP47 affects USP47

| 1

reach

37924851

USP47 affects ptc

| 1

USP47 activates ptc. 1 / 1

| 1

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reach

"USP47 promotion of PTC development may be due to its stabilization of SATB1 protein, suggesting that targeting the USP47/SATB1 signaling axis may serve as a therapeutic intervention for PTC."

37564825

USP47 affects polyQ-induced cell death

| 1

USP47 inhibits polyQ-induced cell death. 1 / 1

| 1

| 1

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reach

"The association between miR-454 and KCNQ1OT1 or USP47 was verified via bioinformatics analysis, dual-luciferase reporter assays and RIP assays."

33576460

USP47 affects math-33 allele

| 1

➶

reach

35509185

USP47 affects TERF1

| 1

| 1

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reach

35024322

USP47 affects STUB1

| 1

USP47 activates STUB1. 1 / 1

| 1

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reach

"USP47 regulates katanin p60-mediated axonal growth with the C-terminus Hsp70-interacting protein (CHIP) ."

37740002

USP47 affects STS

| 1

1 |

USP47 binds TCEA1. 1 / 1

1 |

➶

biogrid

No evidence text available

28514442

TBP affects USP47

1 |

| 1

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sparser

34604226

SMURF2 affects USP47

| 1

USP47 binds SMURF2. 1 / 1

| 1

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reach

"USP47 interacted with SATB1 through its catalytic core domain and antagonized Smurf2-mediated SATB1 degradation."

34604226

SMARCA4 affects USP47

| 1

1 |

DDI2 binds USP47. 1 / 1

1 |

➶

biogrid

No evidence text available

33961781

CUL1 affects USP47

1 |

CUL1 binds USP47. 1 / 1

1 |

➶

biogrid

No evidence text available

35831314

CTNNB1 affects fbxw1

| 1

ctd

No evidence text available

31675489

IndraLab

Statements

USP47 affects BTRC

USP47 binds BTRC.

BTRC binds USP47. 10 / 33

USP47 increases the amount of BTRC.

USP47 increases the amount of BTRC. 2 / 2

USP47 deubiquitinates BTRC.

USP47 deubiquitinates BTRC. 2 / 2

BTRC affects USP47

BTRC binds USP47.

BTRC binds USP47. 10 / 33

BTRC ubiquitinates USP47.

BTRC leads to the ubiquitination of USP47. 3 / 3

USP47 affects SNAI1

USP47 binds SNAI1.

USP47 binds SNAI1. 10 / 18

SNAI1 binds SATB1 and USP47. 1 / 1

USP47 activates SNAI1.

USP47 activates SNAI1. 6 / 6

USP47 increases the amount of SNAI1.

USP47 increases the amount of SNAI1. 5 / 5

USP47 deubiquitinates SNAI1.

USP47 deubiquitinates SNAI1. 3 / 3

USP47 affects cell population proliferation

USP47 activates cell population proliferation.

USP47 activates cell population proliferation. 10 / 27

USP47 inhibits cell population proliferation.

USP47 inhibits cell population proliferation. 5 / 5

USP47 affects SATB1

USP47 binds SATB1.

SATB1 binds USP47. 10 / 13

SNAI1 binds SATB1 and USP47. 1 / 1

USP47 activates SATB1.

USP47 activates SATB1. 8 / 8

USP47 ubiquitinates SATB1.

USP47 ubiquitinates SATB1. 4 / 4

USP47 deubiquitinates SATB1.

USP47 deubiquitinates SATB1. 2 / 2

USP47 decreases the amount of SATB1.

USP47 decreases the amount of SATB1. 2 / 2

USP47 affects CTNNB1

USP47 deubiquitinates CTNNB1.

USP47 deubiquitinates CTNNB1. 10 / 10

USP47 binds CTNNB1.

USP47 binds CTNNB1. 5 / 5

USP47 binds CTNNB1 and fbxw1. 1 / 1

USP47 activates CTNNB1.

USP47 activates CTNNB1. 6 / 6

USP47 increases the amount of CTNNB1.

USP47 increases the amount of CTNNB1. 2 / 2

USP47 ubiquitinates CTNNB1.

USP47 leads to the ubiquitination of CTNNB1. 1 / 1

USP47 affects CDH1

USP47 deubiquitinates CDH1.

USP47 deubiquitinates CDH1. 7 / 7

USP47 activates CDH1.

USP47 activates CDH1. 6 / 6

USP47 decreases the amount of CDH1.

USP47 decreases the amount of CDH1. 3 / 3

USP47 bound to SNAI1 decreases the amount of CDH1. 1 / 1

USP47 bound to SATB1 decreases the amount of CDH1. 1 / 1

USP47 increases the amount of CDH1.

USP47 increases the amount of CDH1. 2 / 2

USP47 binds CDH1.

USP47 binds CDH1. 2 / 2

USP47 ubiquitinates CDH1.

USP47 leads to the ubiquitination of CDH1. 1 / 1

USP47 affects apoptotic process

USP47 inhibits apoptotic process.

USP47 inhibits apoptotic process. 10 / 16

USP47 activates apoptotic process.

USP47 activates apoptotic process. 6 / 6

USP47 affects NLRP3

USP47 activates NLRP3.

USP47 activates NLRP3. 8 / 8

USP47 deubiquitinates NLRP3.

USP47 deubiquitinates NLRP3. 7 / 7

USP47 ubiquitinates NLRP3.

USP47 ubiquitinates NLRP3. 4 / 4