IndraLab

Statements


USP37 affects MYC
4 1 | 3 29 27
USP37 binds MYC.
4 | 1 7 27
4 | 1 7 26

sparser
"USP37‐C‐Myc interactions might play an essential role in keloid pathogenesis, which could be used to design small molecules and peptidyl disruptors."

sparser
"The previously reported deubiquiting enzyme USP37 can directly bind to and deubiquitinate c-Myc, thereby stabilizing c-Myc ( Pan et al., 2015 )."

sparser
"Interestingly, unlike USP28 [ xref ], USP37 directly binds to c-Myc and acts independently of Fbw37."

trips
"Further studies indicate that USP37 directly interacts with c-Myc and deubiquitinates c-Myc in a DUB activity-dependent manner."

sparser
"USP37 interacts directly with MYC to promote its deubiquitination and maintain its protein stability ( xref )."

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sparser
"Further studies indicate that USP37 directly interacts with c-Myc and deubiquitinates c-Myc in a DUB activity-dependent manner."
14-3-3γ binds Hemagglutinins, USP37, and MYC. 1 / 1
| 1

sparser
"We used a cell counting kit-8 (CCK-8) to measure the cell viability and proliferation mediated by the interaction between HA-14-3-3γ and Myc-USP37."
USP37 deubiquitinates MYC.
1 | 2 15
USP37 deubiquitinates MYC. 10 / 18
1 | 2 15

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"USP37 positively regulates the Warburg effect in lung cancer by deubiquitinating c-Myc [108] ."

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"Lastly, USP37 deubiquitinates and stabilizes the proto-oncogene c-Myc and the oncogenic fusion PLZF-RARA, suggesting that inhibition of USP37 DUB activity could have therapeutic potential XREF_BIBR, XREF_BIBR."

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"An early study showed that USP28 deubiquitinates c-Myc via interacting with Fbw7alpha whereas a recent study reveals that USP37 deubiquitinates c-Myc independently of Fbw7 and c-Myc phosphorylation."

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"Importantly, USP37 had been confirmed to accelerate keloid fibroblast proliferation and collagen production by mediating the deubiquitination and stabilization of c-Myc , suggesting that USP37 played positive role in keloid formation."

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"While the role of ubiquitin-specific peptidase 37 (USP37) in lung cancer, where it mediates the deubiquitination and stabilization of c-myc, has been well-documented, its involvement in DLBCL remains unexplored."

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"Further studies indicate that USP37 directly interacts with c-Myc and deubiquitinates c-Myc in a DUB activity dependent manner."

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"The data show that USP37 may be a potential therapeutic target for DLBCL, and that it may enhance the course of the disease by deubiquitinating c-myc via direct interactions with c-myc."

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"USP37 directly deubiquitinates and stabilizes c-Myc in lung cancer."

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"The previously reported deubiquiting enzyme USP37 can directly bind to and deubiquitinate c-Myc, thereby stabilizing c-Myc ( Pan et al., 2015 )."
USP37 activates MYC.
| 4
USP37 activates MYC. 4 / 6
| 4

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"XREF_BIBR - XREF_BIBR Moreover, the USP37 and USP36 can promote tumorigenesis by stabilizing c-Myc."

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"The overexpression of USP37 markedly increases c-Myc abundance by blocking its degradation, whereas the depletion of USP37 promotes c-Myc degradation and reduces c-Myc levels."

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"Moreover, USP37 and USP22 have been shown to increase c-Myc stability via blockade of proteasomal degradation ( Kim et al., 2017 ; Pan et al., 2015 )."

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"Conversely, USP37 and USP29 promote tumorigenesis by stabilizing c-Myc through deubiquitination [22, 23]."
USP37 phosphorylates MYC.
| 1
USP37 leads to the phosphorylation of MYC on T73. 1 / 1
| 1

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"USP37 was shown to directly interact with MYC at the MBIII region and deubiquitinate MYC.112 Although USP37-mediated deubiquitination and stabilization of MYC is independent of either Fbw7 or the phosphorylation of MYC at T58, overexpression of USP37 can block the Fbw7-mediated degradation of MYC."
USP37 increases the amount of MYC.
| 1
USP37 increases the amount of MYC. 1 / 1
| 1

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"The deubiquitinating enzyme USP37 promotes keloid fibroblasts proliferation and collagen production by regulating the c-Myc expression."
USP37 decreases the amount of MYC.
| 1
USP37 decreases the amount of MYC. 1 / 1
| 1

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"The overexpression of USP37 markedly increases c-Myc abundance by blocking its degradation, whereas the depletion of USP37 promotes c-Myc degradation and reduces c-Myc levels."
USP37 affects SNAI1
9 1 | 15 21
USP37 binds SNAI1.
9 | 4 20
9 | 4 20

sparser
"In gastric cancer, overexpression of PLAGL2 facilitates the proliferation and migration of gastric cancer cells and contributes to the process of epithelial–mesenchymal transition (EMT) via the USP37-Snail1 axis [ xref ]."

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"USP37 directly binds, deubiquitinates, and stabilizes SNAI1."

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sparser
"Pleomorphic adenoma gene like-2 (PLAGL2), a zinc finger plag transcription factor, activates the transcription of USP37, a DUB, which subsequently interacts with Snail1 to facilitate its deubiquitination."
USP37 deubiquitinates SNAI1.
1 | 9
USP37 deubiquitinates SNAI1. 10 / 10
1 | 9

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"Studies have shown that PLAGL2 can activate the transcription of the deubiquitinating enzyme USP37 that deubiquitinates and stabilizes Snail family transcriptional repressor 1 (Snail1), finally fostering Snail1-mediated cell proliferation [62]."

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"Our studies demonstrate that a deubiquitinating enzyme (DUB) family member, USP37, can deubiquitinate Snail and prevent degradation of Snail."

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"USP37 deubiquitinates Snail protein, prevents the degradation of Snail, and stabilizes the initially unstable Snail protein."

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"USP37, a member of the deubiquitinase family, can deubiquitinate snail and prevent their degradation, thereby promoting the migration of LC cells."

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"Studies have shown that PLAGL2 can activate the transcription of the deubiquitinating enzyme USP37 that deubiquitinates and stabilizes Snail family transcriptional repressor 1 (Snail1), finally fostering Snail1-mediated cell proliferation [62]."

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"Wu et al. reported that PLAGL2 promoted the proliferation and migration of gastric cancer cells through ubiquitin-specific protease 37 (USP37)-mediated deubiquitylation of the Snail1 protein ."

"<span class="match term0">USP37</span> Promotes Lung Cancer Cell Migration by Stabilizing <span class="match term1">Snail</span> Protein via Deubiquitination"

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"USP13, USP29, and USP37 were reported to promote the metastasis of GC by deubiquitinating and stabilizing Snail [35,36,54,59]."

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"Subsequent sub-screening identified 23 DUBs that interact with SNAI1, out of which USP29, USP36, and USP37 upregulate and reduce polyubiquitination of the SNAI1 protein."
| PMC

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"Microarray analysis of total RNA isolated from stable PLAGL2 knockdown (SGC7901-shRNA) and SGC7901 NC cells elucidated three differentially expressed DUBs, of which only USP37 directly interacted and significantly diminished the ubiquitination of SNAI1."
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USP37 ubiquitinates SNAI1.
| 1
USP37 ubiquitinates SNAI1. 1 / 1
| 1

sparser
"Accordingly, silencing endogenous USP37 promoted Snail1 ubiquitination in the presence or absence of MG132 (Figure xref I-J)."
USP37 increases the amount of SNAI1.
| 1
USP37 increases the amount of SNAI1. 1 / 1
| 1

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"As shown in Fig. 3c and d, knockdown of USP37 significantly decreased Snail1, N-cadherin and Vimentin expression, but increased E-cadherin expression levels."
USP37 decreases the amount of SNAI1.
| 1
USP37 decreases the amount of SNAI1. 1 / 1
| 1

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"Downregulation of USP37 also decreases the expression of Snail."
USP37 is modified
5 | 1 32 8
USP37 is phosphorylated.
5 | 21 8
USP37 is phosphorylated. 10 / 22
| 19 3

sparser
"CDK2 regulates ERK1/2 stability by phosphorylating and activating USP37."

sparser
"A detailed structural analysis of phosphorylated USP37 may be required to reveal this mechanism."

sparser
"CDK1/2 inhibitors, but not CDK4/6 inhibitors, inhibited USP37 phosphorylation ( xref )."

sparser
"As shown in xref , the S628A mutant of USP37 markedly decreased phospho-CDK substrate signaling, suggesting that S628 is a major site for CDK1/2 mediation of USP37 phosphorylation."

sparser
"We found this interesting because USP37 has been implicated in cell proliferation and transformation [ 6, 7 ] as well as in cell-cycle regulation, where during the G1/S transition, CDK2 phosphorylates[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"However, USP37 phosphorylation did not affect its binding to ERK1/2 ( xref )."

sparser
"These findings suggest that the CDK2-mediated phosphorylation of USP37 exerted a vital function in regulating USP37 DUB activity and the USP37-mediated deubiquitination and stabilization of ERK1/2."

sparser
"Meanwhile, USP37 is phosphorylated and activated by CDK2, promoting the removal of polyubiquitin chains from ERK1/2 and resulting in the stabilization of ERK1/2."

rlimsp
"We observed that just as CDC6, the levels of USP37 protein and phosphorylated USP37 decreased in the presence of CDK2 inhibitor ( )."

sparser
"Subsequent phosphorylation of USP37 by either CDK2/cyclin E or CDK2/cyclin A triggers its full DUB activity."
USP37 is phosphorylated on S628. 4 / 4
| 2 2

sparser
"Ser 628 of USP37 was phosphorylated in G1/S, but not mitosis ( Figure 7 E)."

rlimsp
"The USP37 protein becomes fully functional upon its Cyclin A/CDK2-mediated phosphorylation at Ser-628 residue [6] and remains active throughout the S phase upto G2/M boundary."

sparser
"In G1/S, Ser628 of USP37 is phosphorylated by either CDK2/cyclin E or CDK2/cyclin A, and this triggers USP37 full DUB activity."

rlimsp
"A recent study implicates the phosphorylation of Ser-628 of USP37 to protect it from CDH1 mediated ubiquitination [6]."
USP37 is phosphorylated on S650. 3 / 3
3 |

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USP37 is phosphorylated. 2 / 2
| 2

rlimsp
"For example, during the cell cycle, periodic phosphorylation activates USP16 and USP37 [14, 15] but inactivates USP8 through recruitment of 14-3-3 proteins [16]."

rlimsp
"Of interest, USP37 association with Cdh1 and its activity toward cyclin A are both enhanced by its phosphorylation by CDK."
USP37 is phosphorylated on S652. 2 / 2
2 |

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USP37 is phosphorylated on S628. 1 / 1
| 1

rlimsp
"USP37 activity is stimulated by Cdk2-mediated phosphorylation at Ser628 in a positive feedback loop [67]."
USP37 is ubiquitinated.
| 8
USP37 is ubiquitinated. 8 / 8
| 8

sparser
"As shown in xref , compared with cells expressing USP37 WT, more ubiquitinated USP37 was detected in cells expressing the USP37 CS mutant."

sparser
"The level of USP37 ubiquitination was also reduced by mutations in UIM2 or UIM3, suggesting their role in ubiquitination of USP37 itself."

sparser
"This regulation is achieved, in part, by association with the E3 ubiquitin ligases APC CDH1 and SCF βTrCP which drives the ubiquitylation and degradation of USP37 [ xref ], although how USP37 degradation regulates mitotic entry remains to be elucidated."

sparser
"The level of USP37 ubiquitination is also reduced by mutations in UIM2 or UIM3, suggesting that they play a role in the ubiquitination of USP37 itself."
| PMC

sparser
"Ubiquitination of USP37 relied on a KEN box degron located between its C-terminal UIMs ( Figure S4 A)."

sparser
"Furthermore, UIM2 and UIM3, which are located in tandem with an eight–amino acid spacer in the region between the cysteine and histidine boxes, play an essential role in ubiquitin-binding ubiquitination of USP37, and most importantly the full catalytic activity of USP37."
| PMC

sparser
"We believe that inappropriate APC CDH1 activity results in USP37 ubiquitination and degradation, which shifts the balance in favor of cyclin A being a substrate rather than an inhibitor of the APC/C.[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"To assess USP37 ubiquitination, HeLa cell lysates were prepared in NP-40 buffer and denatured by boiling for 10 min after addition of 1% v/v SDS."
USP37 is sumoylated.
| 2
USP37 is sumoylated on K452. 2 / 2
| 2

sparser
"Similarly, the increased SUMOylation of USP37 at its catalytic site K452 correlated with an increase in ubiquitylation of its substrate c-Myc at K148 and K389."

sparser
"We observed an increase in the SUMOylation of USP37 at K452 located in its catalytic domain, a change that was correlated with an increase in the ubiquitylation of c-Myc at K148 and K389."
USP37 is dephosphorylated.
| 1
USP37 is dephosphorylated. 1 / 1
| 1

sparser
"In late mitosis, CDK2 is no longer active, and thus the APC–CDH1 complex reforms and dephosphorylated USP37 switches from an antagonist to a substrate of the APC-CDH1 complex (Fig. xref )."
| PMC
USP37 is degraded.
| 1
USP37 is degraded. 1 / 1
| 1

trips
"Skp1-Cul1-F-box ubiquitin ligase (SCF(βTrCP))-mediated destruction of the ubiquitin-specific protease USP37 during G2-phase promotes mitotic entry."
MYC affects USP37
4 | 1 7 27
4 | 1 7 26

sparser
"USP37‐C‐Myc interactions might play an essential role in keloid pathogenesis, which could be used to design small molecules and peptidyl disruptors."

sparser
"The previously reported deubiquiting enzyme USP37 can directly bind to and deubiquitinate c-Myc, thereby stabilizing c-Myc ( Pan et al., 2015 )."

sparser
"Interestingly, unlike USP28 [ xref ], USP37 directly binds to c-Myc and acts independently of Fbw37."

trips
"Further studies indicate that USP37 directly interacts with c-Myc and deubiquitinates c-Myc in a DUB activity-dependent manner."

sparser
"USP37 interacts directly with MYC to promote its deubiquitination and maintain its protein stability ( xref )."

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sparser
"Further studies indicate that USP37 directly interacts with c-Myc and deubiquitinates c-Myc in a DUB activity-dependent manner."
14-3-3γ binds Hemagglutinins, USP37, and MYC. 1 / 1
| 1

sparser
"We used a cell counting kit-8 (CCK-8) to measure the cell viability and proliferation mediated by the interaction between HA-14-3-3γ and Myc-USP37."
CDK2 affects USP37
1 2 2 | 17 14 2
CDK2 phosphorylates USP37.
1 1 | 10 8 2
CDK2 phosphorylates USP37. 10 / 15
| 8 5 2

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"We found this interesting because USP37 has been implicated in cell proliferation and transformation [6, 7] as well as in cell-cycle regulation, where during the G1/S transition, CDK2 phosphorylates a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"CDK2 regulates ERK1/2 stability by phosphorylating and activating USP37."

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"USP37 is phosphorylated by CDK2 during the G1/S-phase transition (151)."

sparser
"Mechanistically, CDK2 phosphorylates USP37, which is required for USP37 DUB activity."

sparser
"Cyclin A accumulation activates CDK2, which also phosphorylates USP37 to positively reinforce its catalytic activity."

sparser
"We found this interesting because USP37 has been implicated in cell proliferation and transformation [ 6, 7 ] as well as in cell-cycle regulation, where during the G1/S transition, CDK2 phosphorylates[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"USP37, which is no longer phosphorylated by CDK2, switches from an antagonist to a substrate of APC CDH1 and is modified with degradative K11 linked polyubiquitin."

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"Meanwhile, USP37 is phosphorylated and activated by CDK2, promoting the removal of polyubiquitin chains from ERK1/2 and resulting in the stabilization of ERK1/2.USP37 promotes cancer cell growth via ERK1/2 in vitro and in vivo."

sparser
"USP37, which is no longer phosphorylated by CDK2, switches from an antagonist to a substrate of APC CDH1 and is modified with degradative K11-linked polyubiquitin ( Figure 6 )."

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"Subsequent phosphorylation of USP37 by either CDK2 and cyclin E or CDK2 and cyclin A triggers its full DUB activity."
CDK2 phosphorylates USP37 on S628. 6 / 6
1 1 | 2 2

sparser
"The USP37 activity maximizes when Ser628 of USP37 is phosphorylated by CDK2 ( xref ) ( xref )."

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"In G1/S, Ser628 of USP37 is phosphorylated by either CDK2/cyclin E or CDK2/cyclin A, and this triggers USP37 full DUB activity."

sparser
"Mechanistically, CDK2 phosphorylates USP37 at serine 628 (Ser 628 ) and then activates USP37 deubiquitinase activity."

"There is positive reinforcement of this signaling mechanism because phosphorylation of Ser628 by CDK2/cyclin E and CDK2/cyclin A complexes produces maximal USP37 activity"

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"Mechanistically, CDK2 phosphorylates USP37 at serine 628 (Ser ) and then activates USP37 deubiquitinase activity."
CDK2 phosphorylates USP37 on S858. 1 / 1
| 1

sparser
"When the de-ubiquitinating enzyme ubiquitin specific peptidase 37 (USP37) is phosphorylated by cyclin dependent kinase 2 (CDK2) at Ser858 it can bind to SCF- β TrCP for ubiquitination and degradation in G2."
CDK2 activates USP37.
1 | 5 3
CDK2 activates USP37. 9 / 10
1 | 5 3

sparser
"Deubiquitinase USP37 is activated by CDK2 to antagonize APC(CDH1) and promote S phase entry [ xref ]."

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"Since USP37 S 628 exists within a minimum CDK phosphorylation motif (S/T-P) (Dephoure et al., 2008), CDK2 interacts with USP37, and CDK2 is active in G1/S, we investigated whether CDK2 phosphorylates [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"The role of cysteine 350 in USP37 was first illustrated by Huang et al. in 2011, in which they found that CDK2 activates USP37 to antagonize APC (CDH1) and promote S phase entry and that USP37 deubiquitinates CDT1 [ xref ]."
| PMC

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"Overexpression of CDK2 activating cyclin A or E increased USP37 activity toward HA-Ubi-VS, whereas cyclins B and D were without effect, suggesting that USP37 is one of several CDK2 substrates that int[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"We found this interesting because USP37 has been implicated in cell proliferation and transformation [ 6, 7 ] as well as in cell-cycle regulation, where during the G1/S transition, CDK2 phosphorylates[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Deubiquitinase USP37 is activated by CDK2 to antagonize APC (CDH1) and promote S phase entry [XREF_BIBR]."

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"There is positive reinforcement of this signaling mechanism because phosphorylation of Ser 628 by CDK2 and cyclin E and CDK2 and cyclin A complexes produces maximal USP37 activity, and USP37 activates[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"There is positive reinforcement of this signaling mechanism because phosphorylation of Ser628 by CDK2/cyclin E and CDK2/cyclin A complexes produces maximal USP37 activity"

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"Cdk2-dependent phosphorylation enhanced the activity of USP37 toward cyclin A."
CDK2 binds USP37.
2 | 1 3
2 | 1 3

sparser
"Since USP37 S 628 exists within a minimum CDK phosphorylation motif (S/T-P) ( Dephoure et al., 2008 ), CDK2 interacts with USP37 ( Figure 1 C), and CDK2 is active in G1/S, we investigated whether CDK[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In this study, we found that the CDK2-USP37 axis is a novel regulator for ERK1/2 through ERK1/2 stabilization."

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"Since USP37 S 628 exists within a minimum CDK phosphorylation motif (S/T-P) (Dephoure et al., 2008), CDK2 interacts with USP37, and CDK2 is active in G1/S, we investigated whether CDK2 phosphorylates [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Our study resulted in a model of the phosphorylation-deubiquitination cascade referred to as the CDK2USP37 axis that regulates the deubiquitination and stabilization of ERK1/2."

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CDK2 dephosphorylates USP37.
| 1
CDK2 dephosphorylates USP37. 1 / 1
| 1

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"In late mitosis, CDK2 is no longer active, and thus the APC–CDH1 complex reforms and dephosphorylated USP37 switches from an antagonist to a substrate of the APC-CDH1 complex (Fig. 1)."
| PMC
SNAI1 affects USP37
9 | 4 20
9 | 4 20

sparser
"In gastric cancer, overexpression of PLAGL2 facilitates the proliferation and migration of gastric cancer cells and contributes to the process of epithelial–mesenchymal transition (EMT) via the USP37-Snail1 axis [ xref ]."

reach
"USP37 directly binds, deubiquitinates, and stabilizes SNAI1."

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sparser
"Pleomorphic adenoma gene like-2 (PLAGL2), a zinc finger plag transcription factor, activates the transcription of USP37, a DUB, which subsequently interacts with Snail1 to facilitate its deubiquitination."
USP37 affects CDC73
5 | 18 9
USP37 binds CDC73.
5 | 14 9
5 | 14 9

sparser
"We have shown above that CDC73 is polyubiquitinated via K48-linked ubiquitin chains that are deubiquitinated by USP37, and that CDC73 directly binds to USP37, via an interaction between the β-catenin binding region of CDC73 and the ubiquitin-interacting motifs (UIM2 and 3) of USP37."

sparser
"Purified USP37 interacted with CDC73, and vice versa ( xref B,C)."

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"They found that ubiquitin-specific proteases 37 (USP37) directly binds to parafibromin and prevents ubiquitin-induced degradation of parafibromin."

sparser
"Repeated GST pull-down analysis using GST-USP37/pGEX-4T-1 and GST-CDC73/pGEX-4T-1 confirmed a direct interaction between USP37 and CDC73."

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"The biochemical interaction between the USP37 and CDC73 and their reciprocal binding domains were studied."

sparser
"In co-IP assays using the FLAG antibody, the CDC73 mutants did not interact with USP37, whereas wild-type CDC73 did ( xref B)."
USP37 increases the amount of CDC73.
| 2
USP37 increases the amount of CDC73. 2 / 2
| 2

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"When the protein synthesis was blocked with cycloheximide (50 μg/mL), expression of CDC73 was found to be increased by USP37 but not by USP37 (Figure 4B)."

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"Unlike the catalytically inactive form of USP37 , overexpression of USP37 increased the expression level of CDC73 (Figure 4B)."
USP37 deubiquitinates CDC73.
| 2
USP37 deubiquitinates CDC73. 2 / 2
| 2

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"CDC73 Is Deubiquitinated by USP37."

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"USP37 Deubiquitinates CDC73 in HPT-JT Syndrome."
USP37 affects CDT1
3 1 | 2 21 3
USP37 binds CDT1.
3 | 2 3
3 | 2 3

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"USP37 interacted with and deubiquitinated CDT1 to protect it from proteasomal degradation."

sparser
"Therefore, we further examined the 6 enriched genes in this pathway using an online tool (Chipbase) and found that USP37 and CDT1 were closely associated with lung cancer ( xref D)."

sparser
"In order to confirm this hypothesis, we performed a co-immunoprecipitation assay followed by an immunoblotting analysis, detecting CDT1 and USP37, respectively, in order to evaluate the interactions between USP37 and CDT1."

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"In order to confirm this hypothesis, we performed a co-immunoprecipitation assay followed by an immunoblotting analysis, detecting CDT1 and USP37, respectively, in order to evaluate the interactions between USP37 and CDT1."

sparser
"Previous studies have demonstrated that USP37 interacts with CDT1, a key protein in replication fork progression, and stabilizes its phosphorylated form [ xref ]."
USP37 increases the amount of CDT1.
| 8
USP37 increases the amount of CDT1. 8 / 8
| 8

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"Moreover, previous evidence has shown that USP37 promotes the expression of CDT1."

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"If the overexpression of USP37 increases Cdt1 protein levels by affecting its ubiquitination- and proteasomal-dependent degradation, lack of USP37 should have the opposite consequence."

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"Also, overexpression of an siRNA resistant version of Flag-USP37 rescued the drop in Cdt1 levels caused by USP37 depletion ( Supplemental Figure 4A )."

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"Using an overexpression screen of 78 human ubiquitin and ubiquitin-like hydrolases, Perez et al. reported that USP37 increases CDT1 levels when cells are exposed to DNA-damaging agents while USP37 depletion with siRNA decreases CDT1 protein levels [48]."
| PMC

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"The results revealed that the overexpression of USP37 increased CDT1 protein levels and USP37 silencing decreased CDT1 expressions (XREF_FIG I and 5J)."

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"USP37 overexpression increases Cdt1 protein levels and knock down of this deubiquitinating enzyme (DUB) leads to lower Cdt1 levels."

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"To understand whether the regulation of CDT1 expression by USP37 plays a role in lung cancer cell growth and invasion, the expressions of miR-320b and/or CDT1 in lung cancer cells (A549 and H1650) were altered."

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"The results illustrated that USP37 knockdown significantly reduced CDT1 protein levels while lysosomal inhibitor (MG132) preserved the CDT1 protein (XREF_FIG M)."
USP37 deubiquitinates CDT1.
1 | 4
USP37 deubiquitinates CDT1. 5 / 5
1 | 4

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"The role of cysteine 350 in USP37 was first illustrated by Huang et al. in 2011, in which they found that CDK2 activates USP37 to antagonize APC (CDH1) and promote S phase entry and that USP37 deubiquitinates CDT1 [19]."
| PMC

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"USP37 regulates DNA replication by deubiquitinating CDT1, an important protein in DNA replication, but USP37 may control many unknown factors at replication forks that should be further explored [48]."
| PMC

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"USP37 interacted with and deubiquitinated CDT1 to protect it from proteasomal degradation."

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"USP37 induces deubiquitination of CDT1, whereas a catalytically inactive (C350S) USP37 mutant is unable to ubiquitinate CDT1, further suggesting that USP37 plays a role in CDT1 stabilization."
| PMC

"<span class="match term0">USP37</span> deubiquitinates <span class="match term1">Cdt1</span> and contributes to regulate DNA replication"
USP37 decreases the amount of CDT1.
| 3
USP37 decreases the amount of CDT1. 3 / 3
| 3

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"The results revealed that the overexpression of USP37 increased CDT1 protein levels and USP37 silencing decreased CDT1 expressions (XREF_FIG I and 5J)."

reach
"Also, overexpression of an siRNA resistant version of Flag-USP37 rescued the drop in Cdt1 levels caused by USP37 depletion ( Supplemental Figure 4A )."

reach
"USP37 overexpression increases Cdt1 protein levels and knock down of this deubiquitinating enzyme (DUB) leads to lower Cdt1 levels."
USP37 activates CDT1.
| 2 1
USP37 activates CDT1. 3 / 3
| 2 1

eidos
"Moreover , previous evidence has shown that USP37 promotes the expression of CDT1 ."

eidos
"Using an overexpression screen of 78 human ubiquitin and ubiquitin-like hydrolases , Perez et al. reported that USP37 increases CDT1 levels when cells are exposed to DNA-damaging agents while USP37 depletion with siRNA decreases CDT1 protein levels [ 48 ] ."
| PMC

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"Thus, we hypothesized that USP37 may contribute to the tumorigenesis of lung cancer by regulating CDT1."
USP37 ubiquitinates CDT1.
| 2
USP37 ubiquitinates CDT1. 2 / 2
| 2

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"USP37 induces deubiquitination of CDT1, whereas a catalytically inactive (C350S) USP37 mutant is unable to ubiquitinate CDT1, further suggesting that USP37 plays a role in CDT1 stabilization."
| PMC

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"In patients with lung cancer who also experienced OSA, IH induced a decrease in the expression of microRNA-320b, with downstream expression of ubiquitin-specific peptidase 37 (USP37) upregulated secondary to IH and USP37 promoting lung cancer progression by mediating the deubiquitination of Cdc10-dependent transcript 1 (CDT1) (98)."
USP37 inhibits CDT1.
| 1
USP37 inhibits CDT1. 1 / 1
| 1

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"To explore whether USP37 mediates the proteasomal degradation of CDT1, we treated USP37 silencing cells with MG132 or dimethyl sulfoxide (DMSO) and detected the expression of CDT1 afterward."
USP37 affects CDH1
| 17 14
USP37 binds CDH1.
| 10 14
| 9 12

reach
"USP37 binds to APC/C adaptor protein CDH1 in G1/S and removes degradative polyUb from the APC substrate cyclin A. USP37-mediated deubiquitination and stabilization of cyclin A enable entry into the S phase."

sparser
"USP37 binds to APC/C adaptor protein CDH1 in G1/S and removes degradative polyUb from the APC CDH1 substrate cyclin A. USP37-mediated deubiquitination and stabilization of cyclin A enable entry into the S phase."

reach
"In G1/S, Activated USP37 binds to Cdh1 and deubiquitinates cyclin A, which Promote S Phase Entry [XREF_BIBR]."

sparser
"Here we show that USP37 binds the APC/C coactivator CDH1, but not CDC20."

reach
"They identified interactions between USP37 and CDH1, as well as APC/C subunits, implicating USP37 in the regulation of the G1/S transition and characterized the cell cycle regulation of USP37 (Figure 4C)."

sparser
"Knockdown of CDH1 markedly reduced the interaction between USP37 and CDC27 ( Figure 1 F), suggesting that core APC/C components bind USP37 indirectly through CDH1."

sparser
"A direct interaction between USP37 and CDH1 is likely because mixing of USP37 and CDH1 translated individually in rabbit reticulocyte lysates reproduced the interaction observed in cells ( Figure 1 G)[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"They identified interactions between USP37 and CDH1, as well as APC/C subunits, implicating USP37 in the regulation of the G1/S transition and characterized the cell cycle regulation of USP37 ( xref )."

sparser
"Consistent with this degron being targeted by APC CDH1 , the KEN box-3 mutant USP37 interacted poorly with CDH1 ( Figure 5 F) and had a much longer half-life ( Figure 5 G)."

reach
"HBx acts as a chaperone of USP37 and shuttles it out of the nucleus, where the ubiquitin E3 ligase CDC20 homolog 1 (CDH1) and b-TrCP associate with USP37 (Zhou et al., 2003; von Mikecz, 2006; Saxena and Kumar, 2014)."
CDH1 binds CDC20 and USP37. 1 / 1
| 1

sparser
"Huang et al. characterized the USP37 interaction with CDH1 and CDC20 by tandem mass spectrometry and found that USP37 interacts with CDH1 but not CDC20."
| PMC
Mutated USP37 binds CDH1. 1 / 1
| 1

reach
"Consistent with this degron being targeted by APC CDH1, the KEN box-3 mutant USP37 interacted poorly with CDH1 and had a much longer half-life."
| 1

sparser
"Finally, specificity for Cdh1 may introduce possibilities for modulating the degradation of substrates via chain editing – for example, the DUB USP37 interacts with APC/CCdh1 to modulate K11 linkages on at least one substrate ( xref )."
USP37 inhibits CDH1.
| 2
USP37 inhibits CDH1. 2 / 2
| 2

reach
"However, HBx escorts USP37 from the nucleus, downregulates CDH1, and prevents its SCF mediated degradation via increased CDK2-mediated phosphorylation [24]."
| PMC

reach
"A study by Qin et al. reported that USP37 KD suppresses BC and BC stem cell migration and invasion by promoting the mesenchymal-epithelial transition (MET) by markedly reducing the SNAI1, N-cadherin, and vimentin expression and increasing the E-cadherin."
| PMC
USP37 increases the amount of CDH1.
| 2
USP37 increases the amount of CDH1. 2 / 2
| 2

reach
"Here, we showed that USP37 siRNA treatment stimulated the expression of epithelial marker (E-cadherin) but decreased the expression of three known inducers of EMT (Snail1, N-cadherin and Vimentin) (Fig. 6a)."

reach
"As shown in Fig. 3c and d, knockdown of USP37 significantly decreased Snail1, N-cadherin and Vimentin expression, but increased E-cadherin expression levels."
USP37 decreases the amount of CDH1.
| 2
USP37 decreases the amount of CDH1. 2 / 2
| 2

reach
"As shown in Fig. 3c and d, knockdown of USP37 significantly decreased Snail1, N-cadherin and Vimentin expression, but increased E-cadherin expression levels."

reach
"Immunofluorescence assay showed that overexpression of USP37 further inhibited E-cadherin expression and upregulated N-cadherin expression (Fig. 3j)."
USP37 activates CDH1.
| 1
USP37 activates CDH1. 1 / 1
| 1

reach
"Moreover, USP37 contributes to the stabilization of cyclin A by counteracting APC/Cdh1 functioning."
USP37 affects SAMHD1
| 24 4
USP37 inhibits SAMHD1.
| 8
USP37 inhibits SAMHD1. 7 / 7
| 7

reach
"Co-transfection of SAMHD1, SIVmac239, Vpx, and USP37 in HEK293T cells showed that USP37 inhibited the degradation of SAMHD1 by Vpx in a dose-dependent manner (Fig. 2A)."

reach
"Wenyan’s team also reported that deubiquitinase USP37 enhances the anti-HIV-2/SIV ability of the host restriction factor SAMHD1 [33], while USP3 inhibits HIV-1 replication via promoting APOBEC3G expression through both enzyme activity-dependent and -independent manners [34]."

reach
"USP37 reverses the degradation of SAMHD1 mediated by different subtypes of HIV-2/SIV."

reach
"We further determined that USP37 inhibits the degradation of SAMHD1 by HIV-2 Vpx (Fig. 3A)."

reach
"In this study, we demonstrated that deubiquitinase USP37 effectively inhibits the Vpx-mediated degradation and antiviral and anti-retrotransposition activities of SAMHD1 (Fig. 1, 2 and 8)."

reach
"As expected, the presence of USP37 reversed the degradation of SAMHD1 by HIV-2Rod, SIVsmm-L1V2, SIVsmm-L4, and SIVagm viruses, as shown by the positive control SIVmac239 (Fig. 3C)."

reach
"Therefore, USP37 reverses the degradation of SAMHD1 by different HIV-2/SIV subtypes."
USP37 inhibits SAMHD1-T592A. 1 / 1
| 1

reach
"The results showed that USP37 inhibited the degradation of SAMHD1 T592A and T592D induced by Vpx, whereas the USP37 C350A mutant exhibited a significant reduction in the stabilization of SAMHD1 (Fig. 7F)."
USP37 deubiquitinates SAMHD1.
| 8
USP37 deubiquitinates SAMHD1. 5 / 5
| 5

reach
"We further demonstrated that USP37 can deubiquitinate the polyubiquitin of SAMHD1 induced by TRIM21 (Fig. 5)."

reach
"In vitro deubiquitination analysis showed that the polyubiquitination of SAMHD1, whether induced by Vpx or TRIM21, could be deubiquitinated by USP37 purified in Escherichia coli (Fig. 5G and H)."

reach
"Notably, USP37 deubiquitinates SAMHD1 by directly recognizing SAMHD1 rather than by targeting the E3 ubiquitin ligase."

reach
"However, USP37 significantly reduced polyubiquitination of SAMHD1 induced by SIVmac239 Vpx (Fig. 4D, lanes 3 and 4)."

reach
"Overall, USP37 deubiquitinates SAMHD1 by recognizing SAMHD1 rather than recognizing the E3 complex."
USP37 leads to the deubiquitination of mutated SAMHD1. 1 / 1
| 1

reach
"USP37 also deubiquitinates the polyubiquitin of SAMHD1 mutants that cannot be degraded by Vpx."
USP37 deubiquitinates SAMHD1-T592A. 1 / 1
| 1

reach
"The deubiquitination assay also demonstrated that USP37 deubiquitinated both SAMHD1 T592A and T592D (Fig. 7G)."
USP37 deubiquitinates SAMHD1-T592D. 1 / 1
| 1

reach
"The deubiquitination assay also demonstrated that USP37 deubiquitinated both SAMHD1 T592A and T592D (Fig. 7G)."
USP37 activates SAMHD1.
| 6
USP37 activates SAMHD1. 6 / 6
| 6

reach
"Further analysis showed that USP37 restored the inhibitory effect of Vpx on endogenous SAMHD1 (Fig. 8J)."

reach
"Furthermore, USP37 enhances antiviral efficacy and suppresses retrotransposition of LINE-1 elements by stabilizing SAMHD1."

reach
"In summary, USP37 enhances antiviral and anti-retrotransposition activities by stabilizing SAMHD1."

reach
"Deubiquitinase USP37 enhances the anti-HIV-2/SIV ability of the host restriction factor SAMHD1."

reach
"Interestingly, USP37 could enhanced the stability of SAMHD1 in the absence of Vpx or SAMHD1 mutant resistance to Vpx-mediated degradation (Fig. 5B)."

reach
"However, USP37 WT restored the ability of SAMHD1 to inhibit the LINE1 activity, but the C350A mutant only partially restored the SAMHD1 activity (Fig. 8I, lanes 5 and 6)."
USP37 binds SAMHD1.
| 1 4
| 1 4

reach
"Overexpression of SAMHD1-Flag, but not DDB1, DCAF1, CUL4A, or CUL4B, pulled down USP37 in precipitates (Fig. 4C), indicating a specific interaction between USP37 and SAMHD1."

sparser
"USP37 interacts with SAMHD1 and removes polyubiquitination of SAMHD1."

sparser
"Overexpression of SAMHD1-Flag, but not DDB1, DCAF1, CUL4A, or CUL4B, pulled down USP37 in precipitates ( xref ), indicating a specific interaction between USP37 and SAMHD1."

sparser
"Taken together, these results indicate that USP37 specifically interacts with SAMHD1 and removes polyubiquitination of SAMHD1."

sparser
"Our results also showed that USP37 interacted with SAMHD1 but not with the E3 complex recruited by Vpx ( xref )."
USP37 increases the amount of SAMHD1.
| 1
USP37 increases the amount of SAMHD1. 1 / 1
| 1

reach
"We found that USP37 greatly increased the expression of SAMHD1 (Fig. 1A through C)."
CDH1 affects USP37
| 12 16
CDH1 binds USP37.
| 10 14
| 9 12

reach
"USP37 binds to APC/C adaptor protein CDH1 in G1/S and removes degradative polyUb from the APC substrate cyclin A. USP37-mediated deubiquitination and stabilization of cyclin A enable entry into the S phase."

sparser
"USP37 binds to APC/C adaptor protein CDH1 in G1/S and removes degradative polyUb from the APC CDH1 substrate cyclin A. USP37-mediated deubiquitination and stabilization of cyclin A enable entry into the S phase."

reach
"In G1/S, Activated USP37 binds to Cdh1 and deubiquitinates cyclin A, which Promote S Phase Entry [XREF_BIBR]."

sparser
"Here we show that USP37 binds the APC/C coactivator CDH1, but not CDC20."

reach
"They identified interactions between USP37 and CDH1, as well as APC/C subunits, implicating USP37 in the regulation of the G1/S transition and characterized the cell cycle regulation of USP37 (Figure 4C)."

sparser
"Knockdown of CDH1 markedly reduced the interaction between USP37 and CDC27 ( Figure 1 F), suggesting that core APC/C components bind USP37 indirectly through CDH1."

sparser
"A direct interaction between USP37 and CDH1 is likely because mixing of USP37 and CDH1 translated individually in rabbit reticulocyte lysates reproduced the interaction observed in cells ( Figure 1 G)[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"They identified interactions between USP37 and CDH1, as well as APC/C subunits, implicating USP37 in the regulation of the G1/S transition and characterized the cell cycle regulation of USP37 ( xref )."

sparser
"Consistent with this degron being targeted by APC CDH1 , the KEN box-3 mutant USP37 interacted poorly with CDH1 ( Figure 5 F) and had a much longer half-life ( Figure 5 G)."

reach
"HBx acts as a chaperone of USP37 and shuttles it out of the nucleus, where the ubiquitin E3 ligase CDC20 homolog 1 (CDH1) and b-TrCP associate with USP37 (Zhou et al., 2003; von Mikecz, 2006; Saxena and Kumar, 2014)."
CDH1 binds CDC20 and USP37. 1 / 1
| 1

sparser
"Huang et al. characterized the USP37 interaction with CDH1 and CDC20 by tandem mass spectrometry and found that USP37 interacts with CDH1 but not CDC20."
| PMC
Mutated USP37 binds CDH1. 1 / 1
| 1

reach
"Consistent with this degron being targeted by APC CDH1, the KEN box-3 mutant USP37 interacted poorly with CDH1 and had a much longer half-life."
| 1

sparser
"Finally, specificity for Cdh1 may introduce possibilities for modulating the degradation of substrates via chain editing – for example, the DUB USP37 interacts with APC/CCdh1 to modulate K11 linkages on at least one substrate ( xref )."
CDH1 ubiquitinates USP37.
| 2
CDH1 ubiquitinates USP37. 2 / 2
| 2

sparser
"Inactive USP37 is ubiquitinated by E3 ubiquitin ligase APC CDH1 and degraded in the proteasome ( xref )."

sparser
"Further evidence for USP37 deubiquitinating itself is that APC CDH1 could ubiquitinate USP37 in vitro only if the catalytic Cys 350 was mutated or inhibited by ubiquitin-aldehyde ( Figure 6 C)."
CDH1 inhibits USP37.
| 2
CDH1 inhibits USP37. 2 / 2
| 2

reach
"Overexpression of CDH1, but not CDC20, beta-TRCP1, or beta-TRCP2, decreased endogenous USP37 in 293T cells."

reach
"CDH1 promoted USP37 degradation except when the KEN box-3 was mutated."
CDC73 affects USP37
5 | 14 9
5 | 14 9

sparser
"We have shown above that CDC73 is polyubiquitinated via K48-linked ubiquitin chains that are deubiquitinated by USP37, and that CDC73 directly binds to USP37, via an interaction between the β-catenin binding region of CDC73 and the ubiquitin-interacting motifs (UIM2 and 3) of USP37."

sparser
"Purified USP37 interacted with CDC73, and vice versa ( xref B,C)."

No evidence text available

No evidence text available

reach
"They found that ubiquitin-specific proteases 37 (USP37) directly binds to parafibromin and prevents ubiquitin-induced degradation of parafibromin."

sparser
"Repeated GST pull-down analysis using GST-USP37/pGEX-4T-1 and GST-CDC73/pGEX-4T-1 confirmed a direct interaction between USP37 and CDC73."

No evidence text available

No evidence text available

reach
"The biochemical interaction between the USP37 and CDC73 and their reciprocal binding domains were studied."

sparser
"In co-IP assays using the FLAG antibody, the CDC73 mutants did not interact with USP37, whereas wild-type CDC73 did ( xref B)."

reach
"USP37 up-regulation promoted the proliferation, cell cycle progression, apoptosis inhibition, migration, invasion, epithelial mesenchymal transition (EMT) and stemness of CRC cells; moreover, USP37 facilitated the angiogenesis of human umbilical vein endothelial cells (HUVECs)."

reach
"Similar to the observations in 2D, in the 3D culture platform too, the transient inhibition of USP37 decreased the proliferation rate of the encapsulated cells; however, the results were more faithfully mimicking the conditions in vivo."

reach
"XREF_BIBR Therefore, it is possible that IH mediated miR-320b reduction in lung cancer cells resulted in the upregulation of USP37, which further promoted cancer cell proliferation, invasion, and survival."

reach
"When tested on DLBCL cells, USP37 increased cell proliferation and inhibited cell cycle progression."

reach
"Accumulating evidence has demonstrated that USP37 promotes lung cancer cell proliferation, migration, and invasion XREF_BIBR but inhibits lung cancer cell apoptosis in a deubiquitination dependent manner."

reach
"For example, USP2, USP22, USP28, and USP37 stabilize c-Myc expression and, thus, stimulate the proliferation of many tumour cells [58–61] ."

reach
"These results demonstrated that USP37, serving as a potential DUB, de‐polyubiquitylated and stabilized ERα via removing the K48‐linked ubiquitination.3.5 USP37 promotes cell proliferation through ERalpha ."

reach
"USP37 promotes cell proliferation by stabilizing 14-3-3gamma."

reach
"On the other hand, USP37 shRNAs inhibited the proliferation of keloid fibroblasts with diminished c-Myc and Collagen I expression."

reach
"Notably, our results showed that overexpression of USP37, but not USP37 (C350S), enhanced the proliferation of cancer cells when 14-3-3gamma was co-transfected."

reach
"Thus, the conclusion we derived from this study is that USP37 downregulation does decrease the proliferation rate of the cells and this transient effect of USP37 could be successfully captured by the 3D cell culture model.4."

reach
"Since concomitant loss of REST and USP37 expression attenuated p27 stabilization and differentiation and rescued cell proliferation, our data strongly suggest that repression of USP37 and consequent p27 degradation, are important for REST dependent maintenance of cell proliferation."

eidos
"Functionally , downregulated USP37 decreases cell proliferation and anchorage-independent growth [ 28 ] ."
| PMC

reach
"These findings indicate that ectopic USP37 not only blocked REST dependent cell proliferation, but also countered the blockade to neuronal differentiation even in the presence of REST."

reach
"Functionally, USP37 depletion causes decreased cell proliferation measured by MTS, two-dimensional (2D) colony formation as well as three-dimensional (3D) anchorage- independent growth."

reach
"Functionally, downregulated USP37 decreases cell proliferation and anchorage-independent growth [28]."
| PMC
USP37 affects BLM
4 | 19
USP37 binds BLM.
4 | 8
4 | 8

No evidence text available

reach
"Here, we demonstrate that the deubiquitinating enzyme USP37 interacts with BLM and that USP37 deubiquitinates and stabilizes BLM, thereby sustaining the DNA damage response (DDR)."

reach
"Mechanistically, DNA double-strand breaks (DSB) promotes ATM phosphorylation of USP37 and enhances the binding between USP37 and BLM."

reach
"Mechanistically, USP37 is phosphorylated by ATM following DNA damage, which increases the binding between USP37 and BLM and leads to BLM deubiquitylation."

reach
"We found that only HA-tagged USP37 bound with endogenous BLM (Figure 1A)."

reach
"In addition, we examined the endogenous interaction between USP37 and BLM in HEK293T cells and confirmed that USP37 interacted with BLM (Figure 1C, D)."

reach
"To support this notion, we found that the interaction between USP37 and BLM was enhanced upon cisplatin treatment in cells (Figure 4D)."

reach
"Collectively, these results indicate that DNA damage can strengthen the interaction between USP37 and BLM, which in turn stabilizes BLM."

reach
"We found that S114A mutation attenuated the interaction between USP37 and BLM (Figure 5E)."

No evidence text available
USP37 deubiquitinates BLM.
| 7
USP37 deubiquitinates BLM. 7 / 7
| 7

reach
"Correction to 'USP37 regulates DNA damage response through stabilizing and deubiquitinating BLM'."

reach
"Here, we demonstrate that the deubiquitinating enzyme USP37 interacts with BLM and that USP37 deubiquitinates and stabilizes BLM, thereby sustaining the DNA damage response (DDR)."

reach
"Moreover, knockdown of USP37 increases BLM polyubiquitination, accelerates its proteolysis, and impairs its function in DNA damage response."

reach
"USP37 deubiquitinates BLM in vivo and in vitro."

reach
"In addition, WT USP37, but not the CA mutant, decreased BLM polyubiquitination in vitro (Figure 3D, and Supplementary Figure S2A, B)."

reach
"Since USP37 inhibits the ubiquitination of BLM following DNA damage, we further explored whether and how USP37 itself is regulated following DNA damage."

reach
"In this study, we demonstrate that USP37 deubiquitinates and stabilizes BLM."
USP37 activates BLM.
| 4
USP37 activates BLM. 4 / 4
| 4

reach
"Mechanistically, DNA damage activates ATM which phosphorylates USP37 and activates its deubiquitinase activity to stabilize BLM."

reach
"USP37 promotes BLM stabilization."

reach
"USP37-promoted BLM stabilization is potentiated by DNA damage."

reach
"Interestingly, WT USP37 was phosphorylated by ATM, which in turn increased its deubiquitination activity towards BLM, while the S114A mutant did not have this effect."

eidos
"USP37 is a modulator of the epithelial-mesenchymal transition and metastasis ."
| PMC

reach
"In contrast, USP37 upregulation promotes EMT, migration, and invasion [39]."
| PMC

reach
"Therefore, USP37 promotes EMT via the SHH pathway, and the signalling outcomes are determined by the balance of activated and inhibitory GLI1 proteins [39]."
| PMC

reach
"Snail protein is an essential positive regulator of EMT, which is upregulated by USP37."

reach
"Thus, the literature survey and also TCGA database analyses signify that USP37 is upregulated in different types of cancers and the role of USP37 in modifying the EMT by stabilizing the EMT modifier SNAIL has been reported."

reach
"USP37 also participates in the sonic hedgehog (SHH) pathway and the stability of GLI1 protein and promotes EMT."

reach
"Initial studies have shown that in other cancers USP37 directly regulates the stability of EMT regulating proteins like SNAIL."

eidos
"The knockdown of USP37 could inhibit the stemness , cell invasion and EMT via downregulation of Hedgehog pathway ."

reach
"USP37 up-regulation promoted the proliferation, cell cycle progression, apoptosis inhibition, migration, invasion, epithelial mesenchymal transition (EMT) and stemness of CRC cells; moreover, USP37 facilitated the angiogenesis of human umbilical vein endothelial cells (HUVECs)."

reach
"USP37 in breast cancer was reported to increase cell stemness, epithelial‐mesenchymal transition (EMT), and invasion, while to decrease the sensitivity to cisplatin."

reach
"Our results indicated that inhibition of USP37 decreases the migratory potential of cells as compared to control cells and even after 48 hours no significant migration was observed and the scratch was not filled in both PA 1 and IGROV-1 cells in which USP37 was depleted (Fig. 2C and D).3.3 Inhibition of USP37 and its effects on different EMT markers."

reach
"Downregulation of USP37 inhibits stemness, cell invasion and EMT via hedgehog signaling pathway in breast cancer."
USP37 affects NFE2L2
| 16 6
USP37 binds NFE2L2.
| 3 6
| 3 6

reach
"To gain further insight into the mechanism underlying NRF2 protein stability regulation by USP37, we conducted experiments to examine the interaction between USP37 and NRF2."

reach
"The results demonstrated reciprocal interactions between USP37 and NRF2 (Fig. 3a, b)."

reach
"The interaction between USP37 and NRF2 facilitates the removal of ubiquitin chains from NRF2, thereby stabilizing the NRF2 protein."

sparser
"Overall, our findings manifested the significant involvement of the USP37-NRF2 axis in regulating therapeutic interventions for HCC."

sparser
"The interaction between USP37 and NRF2 facilitates the removal of ubiquitin chains from NRF2, thereby stabilizing the NRF2 protein."

sparser
"USP37 interacts with NRF2 and enhances NRF2 deubiquitination."

sparser
"To gain further insight into the mechanism underlying NRF2 protein stability regulation by USP37, we conducted experiments to examine the interaction between USP37 and NRF2."

sparser
"The results demonstrated reciprocal interactions between USP37 and NRF2 (Fig.  xref a, b)."

sparser
"Additionally, USP37 interacts with NRF2 and facilitates its deubiquitination."
USP37 activates NFE2L2.
| 5
USP37 activates NFE2L2. 5 / 5
| 5

reach
"Mechanistically, USP37 modulates the stability of NRF2 through enzymatic activity-dependent deubiquitination."

reach
"Additionally, knocking down USP37 caused NRF2 protein level downregulation (Fig. 1e)."

reach
"Figure 1f depicts that USP37 expression significantly promoted NRF2 protein stability."

reach
"Additionally, USP37, rather than its enzymatically inactive mutant, promoted NRF2 protein stability (Fig. 2c and Figure S2a)."

reach
"This, in turn, activates the NRF2-ARE signaling pathway, which has been associated with chemotherapy resistance.Our findings suggest that the NRF2-ARE signaling pathway activation, regulated by USP37-mediated NRF2 stabilization, may be responsible for the observed chemotherapy resistance in hepatoma cells."
USP37 increases the amount of NFE2L2.
| 3
USP37 increases the amount of NFE2L2. 3 / 3
| 3

reach
"Herein, the wild-type USP37, but not the enzymatically inactive mutant USP37-C350S, increased NRF2 protein levels (Fig. 2a)."

reach
"Furthermore, USP37 expression raised endogenous NRF2 protein levels without affecting NRF2 RNA expression (Fig. 1c, d)."

reach
"We discovered that the expression of one particular DUB, USP37, significantly upregulated the NRF2 protein level (Fig. 1b)."
USP37 deubiquitinates NFE2L2.
| 3
USP37 deubiquitinates NFE2L2. 3 / 3
| 3

reach
"USP37 was found to interact with NRF2 and facilitate the deubiquitination of NRF2."

reach
"USP37 interacts with NRF2 and enhances NRF2 deubiquitination."

reach
"The researchers also proposed that the mechanism underlying USP37-mediated chemoresistance involves the deubiquitination and stabilization of NRF2."
USP37 ubiquitinates NFE2L2.
| 1
USP37 ubiquitinates NFE2L2. 1 / 1
| 1

reach
"By deubiquitinating NRF2, USP37 prevents its degradation, leading to increased NRF2 protein levels."
USP37 inhibits NFE2L2.
| 1
USP37 inhibits NFE2L2. 1 / 1
| 1

reach
"In addition, the researchers examined whether USP37 overexpression could reverse NRF2 downregulation triggered by Sorafenib."
USP37 affects Snail1
| 21
USP37 binds Snail1.
| 12
USP37 binds Snail1. 10 / 12
| 12

reach
"USP37 interacts with and deubiquitinates Snail1 directly."

reach
"We further investigated the interaction between USP37 and Snail1."

reach
"Given that the SRD domain is essential for the interaction between USP37 and Snail1, we evaluated its effect on Snail1 ubiquitination in HEK293T cells."

reach
"Therefore, the SRD domain is also crucial for USP37 mediated Snail1 deubiquitination and the interaction between USP37 and Snail1."

reach
"Overall, these results demonstrate that USP37 interacts with and deubiquitinates Snail1 directly."

reach
"To explore whether the interaction between Snail1 and USP37 requires Snail1 phosphorylation, we utilized CIP to remove phosphate groups from the substrate proteins."

reach
"Strikingly, Co-IP experiments showed that the interaction between USP37 and Snail1 was strongly impeded by CIP intervention."

reach
"Since GSK-3beta is the major kinase involved in Snail1 phosphorylation in the SRD region, the GSK-3beta inhibitor LiCl was used to assess GSK-3beta inhibition in the interaction of USP37 and Snail1."

reach
"As shown in Figure XREF_FIG B, LiCl treatment also significantly disrupted the USP37 and Snail1 interaction."

reach
"Co-IP results showed that GSK-3beta siRNAs diminished approximately 60% of the interaction of USP37 and Snail1."
USP37 deubiquitinates Snail1.
| 6
USP37 deubiquitinates Snail1. 5 / 5
| 5

reach
"Although PLAGL2 does not function as a deubiquitinase to impede Snail1 ubiquitination, PLAGL2 transcriptionally activates USP37, which finally interacts with and deubiquitinates Snail1 directly."

reach
"USP37 interacts with and deubiquitinates Snail1 directly."

reach
"Among these three DUBs, only USP37 significantly diminished the ubiquitination of Snail1, suggesting that USP37 may be a potential deubiquitinase of Snail1."

reach
"Overall, these results demonstrate that USP37 interacts with and deubiquitinates Snail1 directly."

reach
"We also determined that USP37 interacts with and deubiquitinates Snail1 directly, which is transcriptionally activated by PLAGL2."
Modified USP37 leads to the deubiquitination of Snail1. 1 / 1
| 1

reach
"Overexpression of USP37 significantly reduced the endogenous ubiquitination of Snail1 in the AGS cells."
USP37 activates Snail1.
| 3
USP37 activates Snail1. 3 / 3
| 3

reach
"Furthermore, we confirmed the role of GSK-3beta in the USP37 mediated stabilization of Snail1."

reach
"Besides, we showed that PLAGL2 directly activates USP37 expression, which targets Snail1 to proteasome ubiquitination degradation."

reach
"USP37 considerably increased the stability and half-life of Snail1 in the AGS cells."
USP37 affects PCNA
2 | 9 10
USP37 binds PCNA.
2 | 7 10
2 | 7 10

reach
"Various molecular assays, including colony formation, immunofluorescence, immunoprecipitation, and DNA replication restart, were employed to examine the physical interaction between USP37 and PCNA, as well as its physiological effects in osteosarcoma cells."

reach
"Additionally, molecular docking studies were conducted to gain insight into the nature of the interaction between USP37 and PCNA."

reach
"The interaction between USP37 and PCNA is involved in the regulation of replication stress, and disrupting it could potentially trigger synthetic lethality in osteosarcoma."

reach
"We observed increased stabilization of PCNA in cells overexpressing USP37 (Fig. 6B) compared to cells without USP37 overexpression (Fig. 6A), indicating a potential interaction between USP37 and PCNA To test this hypothesis, we treated cells with two cytotoxic agents, HU and cisplatin, and performed immunoprecipitation using anti-USP37 antibody, which was subsequently probed with anti-PCNA antibody."

reach
"Validation of USP37 interaction with PCNA using molecular docking."

reach
"This interaction between USP37 and PCNA plays a vital role in stabilizing different replication factors at the replication fork."

reach
"Our findings show that in osteosarcoma cells, USP37's interaction with PCNA plays a role in supporting the stability of the replication fork."

sparser
"This interaction between USP37 and PCNA plays a vital role in stabilizing different replication factors at the replication fork."

No evidence text available

sparser
"Our study has found that USP37 interacts with PCNA, a central replication factor, and has a higher affinity for PCNA-DNA complex than for PCNA alone."
USP37 deubiquitinates PCNA.
| 2
USP37 deubiquitinates PCNA. 2 / 2
| 2

reach
"We investigated whether USP37 is involved in the deubiquitination of PCNA, as it was shown to interact with PCNA."

reach
"We hypothesized that USP37 may be responsible for deubiquitinating PCNA."
X affects USP37
| 20
X binds USP37.
| 7
USP37 binds X. 7 / 7
| 7

reach
"Our co-immunoprecipitation and confocal microscopic studies suggested a direct interaction between USP37 and HBx."

reach
"Further, we observed that HBx interacted with USP37 and chaperoned it out of nucleus to prevent its ubiquitination and degradation by E3 ubiquitin ligases."

reach
"The dichotomy in the action of two E3 ligases targeting USP37 further inspired us to investigate the possibility of direct or indirect interaction between HBx and USP37 which could reveal a bigger role of HBx in protection of USP37."

reach
"HBx interacts with USP37 and translocates it to cytoplasm."

reach
"Since, HBx and USP37 co-localized in cells, we next examined the possibility of a physical interaction between HBx and USP37."

reach
"Venturing further we explored the possibility of a physical interaction between HBx and USP37."

reach
"This study has relied on the cell culture based system where HBx was co-expressed along with USP37-DD or USP37 either in immortalized human hepatocytes or in hepatoma Huh7 cells to elucidate the oncogenic cooperation between HBx and USP37."
X activates USP37.
| 6
X activates USP37. 6 / 6
| 6

reach
"Conversely, HBx rescued USP37 from CDH1 mediated down-regulation similar to CDC6 but not the other CDH1 substrate, Geminin XREF_BIBR (XREF_FIG)."

reach
"Ironically, Emi1 overexpression did not lead to USP37 accumulation thereby mitigating the possible role of Emi1 in HBx mediated USP37 stabilization."

reach
"HBx of HBV could upregulate USP37 transcripts in both hepatic and non-hepatic cells and also prevents USP37 from proteasomal degradation."

reach
"Many recent studies have highlighted the significant role of E3 ubiquitin ligases, deubiquitinases or substrate translocation between cell compartments, leading to substrate stability motivated us to explore the effect of HBx driven exodus of USP37 from the nucleus vis-a-vis its intracellular stability."

reach
"Thus, we wondered if the effect of DUB inhibitor on SIRT7 protein levels observed in presence of HBx was dependent upon HBx mediated up-regulation of USP37."

reach
"The HBx dependent up-regulation of USP37 could be seen even in non hepatic cells such as HEK293T and U2OS."
X increases the amount of USP37.
| 3
X increases the amount of USP37. 3 / 3
| 3

reach
"HBx augments the expression of USP37."

reach
"HBx was also found to stimulate the gene transcription and protein expression of USP37."

reach
"Thus, by stabilizing the expression of USP37, HBx promotes the accumulation of cyclin A to modulate cell cycle progression."
X inhibits USP37.
| 2
X inhibits USP37. 2 / 2
| 2

reach
"As expected, overexpression of HBx unlike NESM mutant abrogated the interaction of USP37 with its cognate E3 ligases- CDH1 and beta-TrCP (XREF_FIG)."

reach
"HBx of HBV could upregulate USP37 transcripts in both hepatic and non-hepatic cells and also prevents USP37 from proteasomal degradation."
X ubiquitinates USP37.
| 1
X leads to the ubiquitination of USP37. 1 / 1
| 1

reach
"HBx directly attenuated the ubiquitination and subsequent proteasomal degradation of USP37 which could be reversed by RNA interference against HBx."
X deubiquitinates USP37.
| 1
X leads to the deubiquitination of USP37. 1 / 1
| 1

reach
"As HBx could effectively prevent the ubiquitination of USP37, we assessed the regulation of its E3 ubiquitin ligases- CDH1 and beta-TrCP in the presence of HBx."
USP37 affects WAPL
3 | 9 8
USP37 binds WAPL.
3 | 5 8
3 | 5 7

sparser
"Lastly, mutation of UIM2 and/or UIM3 perturbed USP37 binding to the cohesin regulator WAPL and to endogenous Ub-protein conjugates xref , xref ."

sparser
"Because the interaction between USP37 and WAPL is dependent on the ubiquitin-binding domains of USP37, we hypothesized that USP37 may regulate WAPL’s association with chromatin and/or its stability th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"USP37 interacts with WAPL, a negative regulator of cohesion, through its UIMs and regulates the stability of chromatin-associated WAPL."

sparser
"However, because both USP37 and WAPL negatively regulate cohesion, we hypothesized that USP37 may interact with WAPL to facilitate sister chromatid resolution."

sparser
"USP37 -binding mutants of WAPL revealed that the UIM2 and UIM3 domains are essential for interaction with WAPL."
| PMC

sparser
"Indeed, GFP-WAPL co-immunoprecipitates with FLAG-USP37 in HEK293 cells ( Figure 3 D), indicating that WAPL associates with USP37."

reach
"WAPL is pulled down with His Ub under denaturing conditions in all tested treatments, indicating that ubiquitylated WAPL is detected throughout the cell cycle.We expected that, if USP37 deubiquitylate[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

No evidence text available

reach
"However, because both USP37 and WAPL negatively regulate cohesion, we hypothesized that USP37 may interact with WAPL to facilitate sister chromatid resolution."

No evidence text available
| 1

sparser
"The authors further demonstrated that USP37 interacts with both cohesin and a negative regulator WAPL, which is required for releasing cohesin from chromatid arms in prophase."
USP37 deubiquitinates WAPL.
| 3
USP37 deubiquitinates WAPL. 3 / 3
| 3

reach
"Next, to investigate whether USP37 can directly deubiquitylate WAPL, we purified full-length USP37 WT and USP37 C350A from Sf9 insect cells and added either USP37 WT, USP37 C350A, or buffer alone to a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Importantly, when USP37 WT, but not USP37 C350A, is added, ubiquitylation is reduced, indicating that USP37 can directly deubiquitylate WAPL and/or WAPL associated proteins in vitro."

reach
"WAPL is pulled down with His Ub under denaturing conditions in all tested treatments, indicating that ubiquitylated WAPL is detected throughout the cell cycle.We expected that, if USP37 deubiquitylate[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP37 activates WAPL.
| 1
USP37 activates WAPL. 1 / 1
| 1

reach
"Given the critical role of WAPL in the prophase pathway, it will be interesting to discern whether USP37 contributes to other WAPL dependent processes, such as chromatin structure or protecting cohesi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP37 affects ERK
| 15 5
USP37 binds ERK.
| 4 5
| 4 5

sparser
"As shown in xref , the N-terminal region of USP37 (1–330 aa) was critical for the interaction between USP37 and ERK1/2."

sparser
"To investigate the biological function of the USP37-ERK1/2 axis in lung cancer cells in vivo, shRNAs were stably transfected into NCI-A549 cells, which were subcutaneously implanted into nude mice and monitored for tumor growth."

reach
"A coimmunoprecipitation assay was then performed to confirm the interaction between endogenous USP37 and ERK1/2."

reach
"The interaction between USP37 and ERK1/2 was further confirmed using a reciprocal coimmunoprecipitation assay (Fig. 3 D)."

reach
"In addition, the binding between USP37 and ERK1/2 was further confirmed using bacterially expressed GST-USP37 proteins (Fig. 3 E)."

reach
"As shown in Fig. 3 F, the N-terminal region of USP37 (1–330 aa) was critical for the interaction between USP37 and ERK1/2."

sparser
"USP37 interacts with ERK1/2."

sparser
"A coimmunoprecipitation assay was then performed to confirm the interaction between endogenous USP37 and ERK1/2."

sparser
"The interaction between USP37 and ERK1/2 was further confirmed using a reciprocal coimmunoprecipitation assay ( xref )."
USP37 deubiquitinates ERK.
| 6
USP37 deubiquitinates ERK. 6 / 6
| 6

reach
"Next, we examined whether USP37 deubiquitinates ERK1/2."

reach
"As shown in Fig. 4 H, overexpression of wild-type USP37, but not the USP37 CS mutant, decreased ERK1/2 ubiquitination."

reach
"Since USP37 could deubiquitinate and stabilize ERK1/2, we next examined whether CDK2 could affect the USP37-mediated deubiquitination of ERK1/2."

reach
"Mechanistically, depletion of CDK2 or treatment with CDK1/2 inhibitors decreased ERK1/2 protein levels through USP37, which deubiquitinates and stabilizes ERK1/2."

reach
"USP37 deubiquitinates and stabilizes ERK1/2."

reach
"Given that USP37 is a deubiquitinase and interacts with ERK1/2, it is possible that USP37 could deubiquitinate and stabilize ERK1/2."
USP37 increases the amount of ERK.
| 3
USP37 increases the amount of ERK. 3 / 3
| 3

reach
"However, depletion of USP37 abolished the differential expression of ERK1/2 in different phases of the cell cycle (Fig. S4 B), suggesting that the cell cycle regulates ERK1/2 expression in a USP37-dependent manner."

reach
"Moreover, overexpression of USP37 WT or USP37 SD, but not the USP37 CS or USP37 SA mutants, restored the ERK1/2 protein level in cells treated with CDK1/2i (Fig. S3 J)."

reach
"In addition, reconstitution of USP37 partially rescued ERK1/2 protein levels or tumor cell growth in either CDK2-knockdown or CDK1/2i-treated cells (Fig. S4, H–K)."
USP37 decreases the amount of ERK.
| 2
USP37 decreases the amount of ERK. 2 / 2
| 2

reach
"As shown in Fig. 4, A–C, the knockdown of USP37 decreased ERK1/2 protein levels but not ERK1/2 mRNA levels."

reach
"Additionally, CDK1/2 inhibitors decreased ERK1/2 protein levels in control cells but not USP37-depleted cells, suggesting that CDK2 may regulate ERK1/2 protein levels through USP37 (Fig. 6 B; and Fig. S3, B and C)."
PCNA affects USP37
2 | 7 10
2 | 7 10

reach
"Various molecular assays, including colony formation, immunofluorescence, immunoprecipitation, and DNA replication restart, were employed to examine the physical interaction between USP37 and PCNA, as well as its physiological effects in osteosarcoma cells."

reach
"Additionally, molecular docking studies were conducted to gain insight into the nature of the interaction between USP37 and PCNA."

reach
"The interaction between USP37 and PCNA is involved in the regulation of replication stress, and disrupting it could potentially trigger synthetic lethality in osteosarcoma."

reach
"We observed increased stabilization of PCNA in cells overexpressing USP37 (Fig. 6B) compared to cells without USP37 overexpression (Fig. 6A), indicating a potential interaction between USP37 and PCNA To test this hypothesis, we treated cells with two cytotoxic agents, HU and cisplatin, and performed immunoprecipitation using anti-USP37 antibody, which was subsequently probed with anti-PCNA antibody."

reach
"Validation of USP37 interaction with PCNA using molecular docking."

reach
"This interaction between USP37 and PCNA plays a vital role in stabilizing different replication factors at the replication fork."

reach
"Our findings show that in osteosarcoma cells, USP37's interaction with PCNA plays a role in supporting the stability of the replication fork."

sparser
"This interaction between USP37 and PCNA plays a vital role in stabilizing different replication factors at the replication fork."

No evidence text available

sparser
"Our study has found that USP37 interacts with PCNA, a central replication factor, and has a higher affinity for PCNA-DNA complex than for PCNA alone."
| 3 15
| 3 13

reach
"XREF_BIBR Therefore, it is possible that IH mediated miR-320b reduction in lung cancer cells resulted in the upregulation of USP37, which further promoted cancer cell proliferation, invasion, and survival."

reach
"Further studies indicated that USP37 knockdown could inhibit the stemness, cell invasion and EMT in breast cancer via downregulation of Hh pathway."

reach
"The knockdown of USP37 could inhibit the stemness, cell invasion and EMT via downregulation of Hedgehog pathway."

reach
"Moreover, we found that knockdown of USP37 suppressed cell migration and invasion in breast cancer cells."

reach
"We previously demonstrated that ubiquitin carboxyl-terminal hydrolase 37 (UCH37), a member of the DUBs, promoted invasion and postoperative recurrence of HCC, and pre-mRNA processing factor 19 (Prp19) may function as its downstream effecter [XREF_BIBR]."

reach
"USP37 knockdown suppresses breast cancer cell migration and invasion by promoting mesenchymal-epithelial transition."

reach
"On the contrary, upregulation of USP37 promoted EMT, migration and invasion."

eidos
"In contrast , USP37 upregulation promotes EMT , migration , and invasion [ 39 ] ."
| PMC

reach
"Furthermore, upregulation of USP37 markedly promoted invasion and migration of breast cancer cells (Fig. 3k and l)."

reach
"In contrast, USP37 upregulation promotes EMT, migration, and invasion [39]."
| PMC

reach
"Downregulation of USP37 inhibits stemness, cell invasion and EMT via hedgehog signaling pathway in breast cancer."

reach
"A study by Qin et al. reported that USP37 KD suppresses BC and BC stem cell migration and invasion by promoting the mesenchymal-epithelial transition (MET) by markedly reducing the SNAI1, N-cadherin, and vimentin expression and increasing the E-cadherin."
| PMC
USP37 affects NPPA
| 17
| 17

sparser
"This approach is commonly used to compare the performance of two products ( USP37-NF32, 2014 )."

sparser
"Distinct testing devices are described under USP chapter <1724> ( USP37-NF32, 2014a ), representing either self-standing apparatus (vertical diffusion cells) or adaptions to the standard dissolution e[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Functionality is a broad, qualitative, and descriptive term for the general purpose or role an excipient serves in a formulation ( Sheehan, 2011; USP37-NF32, 2014a )."

sparser
"Given that in vitro release tests with synthetic membranes do not directly measure drug bioavailability ( USP37-NF32, 2014c ), this finding confirmed that enhancer cells are equally suitable for detec[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Moreover, although not a direct indication of drug bioavailability ( USP37-NF32, 2014c ), in vitro release data can provide useful information on the role of various factors, such as solubility of ACF[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Topical drug products include semi-solid dosage forms such as creams, ointments, lotions and gels ( USP37-NF32, 2014b )."

sparser
"Diluents are incorporated into tablet or capsule dosage forms to increase dosage form volume or weight, and as such they can also be referred to as fillers ( USP37-NF32, 2014a )."

sparser
"According to the US Pharmacopeia (USP), the limit of these heavy metal content in drugs and dietary supplements is defined as the oral permitted daily exposure (PDE) for: Cd 25 μg/day, Pb 5 μg/day, an[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Although the compaction properties of tablet diluents are considered as relevant, and guidelines can be found to assess tablet strength ( Podczeck, 2012; USP37-NF32, 2014b ), no recommendations are cu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"However, other methods are also described ( USP37-NF32, 2014 )."
WAPL affects USP37
3 | 5 8
3 | 5 7

sparser
"Lastly, mutation of UIM2 and/or UIM3 perturbed USP37 binding to the cohesin regulator WAPL and to endogenous Ub-protein conjugates xref , xref ."

sparser
"Because the interaction between USP37 and WAPL is dependent on the ubiquitin-binding domains of USP37, we hypothesized that USP37 may regulate WAPL’s association with chromatin and/or its stability th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"USP37 interacts with WAPL, a negative regulator of cohesion, through its UIMs and regulates the stability of chromatin-associated WAPL."

sparser
"However, because both USP37 and WAPL negatively regulate cohesion, we hypothesized that USP37 may interact with WAPL to facilitate sister chromatid resolution."

sparser
"USP37 -binding mutants of WAPL revealed that the UIM2 and UIM3 domains are essential for interaction with WAPL."
| PMC

sparser
"Indeed, GFP-WAPL co-immunoprecipitates with FLAG-USP37 in HEK293 cells ( Figure 3 D), indicating that WAPL associates with USP37."

reach
"WAPL is pulled down with His Ub under denaturing conditions in all tested treatments, indicating that ubiquitylated WAPL is detected throughout the cell cycle.We expected that, if USP37 deubiquitylate[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

No evidence text available

reach
"However, because both USP37 and WAPL negatively regulate cohesion, we hypothesized that USP37 may interact with WAPL to facilitate sister chromatid resolution."

No evidence text available
| 1

sparser
"The authors further demonstrated that USP37 interacts with both cohesin and a negative regulator WAPL, which is required for releasing cohesin from chromatid arms in prophase."
USP37 affects Ubiquitin
| 7 9
USP37 binds Ubiquitin.
| 9

sparser
"One possibility is that phosphorylation enhances USP37 binding to its ubiquitinated substrates by altering the configuration of its three ubiquitin-interacting motifs (UIMs) spanning Ser 704 -Ser 723 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Detecting endogenous ubiquitin-protein conjugates that coimmunoprecipitate with transfected wild-type and UIM mutant USP37 proteins suggested only UIM2 and UIM3, rather than UIM1, permit the interaction between USP37 and ubiquitin-protein conjugates in a synergistic manner."
| PMC

sparser
"To determine whether the physical association between USP37 and WAPL is dependent on the ubiquitin-binding activity of USP37, the same mutations were introduced into full-length FLAG-USP37 and express[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"The deubiquitinase activity of USP37 and its ubiquitin interacting motifs are essential for the deubiquitination of SAMHD1, whereas the phosphorylation state of USP37 does not influence its activity."

sparser
"Because USP37 stabilizes chromatin-associated WAPL and the ubiquitin-binding activities of USP37 are important for its association with WAPL, we next aimed to determine whether USP37 regulates deubiqu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In the case of K48-linked substrates, binding of the UIMs to the proximal Ub increased catalytic efficiency of USP37 largely through k cat and to a lesser degree through K M . This result was somewhat counter-intuitive since we expected that binding of the UIMs of USP37 to Ub to have a more pronounced effect on K M (a proxy for binding affinity) since others have shown that UIM mutations perturbed the ability to pull down Ub conjugates xref ."

sparser
"Mutations in UIM2 and/or UIM3 perturb USP37 binding to endogenous ubiquitin–protein conjugates [ xref , xref , xref ]."

sparser
"The issue of how the binding of the UIMs of USP37 to the proximal Ub of K48 di-Ub enhances activity remains an open question."

sparser
"We expected that, if USP37 deubiquitylates WAPL, the ubiquitin-binding activities of USP37 would be critical for the interaction between USP37 and WAPL."
USP37 inhibits Ubiquitin.
| 5
| 4

reach
"Recent studies have demonstrated that ubiquitin-mediated degradation of c-Myc was inhibited by USPs including USP28, USP37, and USP36 [ 18–20 ]."

reach
"Previous studies reported that USP37 increased the Warburg effect and cell viability by repressing ubiquitin mediated degradation of c-Myc XREF_BIBR."

reach
"It was illustrated that USP37 efficiently eliminated the K48‐linked ubiquitin chain from ERα, while the K63‐linked ubiquitin chain on ERα was not affected by USP37 (Figure 5D,E)."

reach
"USP26 or USP37 antagonize ubiquitin dependent spreading of RAP80-BRCA1."
USP37 bound to CDC73 inhibits Ubiquitin. 1 / 1
| 1

reach
"They found that ubiquitin-specific proteases 37 (USP37) directly binds to parafibromin and prevents ubiquitin-induced degradation of parafibromin."
USP37 activates Ubiquitin.
| 2
| 2

reach
"These results suggested that the UIMs in USP37 contribute to the full enzymatic activity, but not ubiquitin chain substrate specificity, of USP37 possibly by holding the ubiquitin chain substrate in the proximity of the catalytic core."

reach
"In the context of DNA double-strand breaks (DSBs), USP37 and USP26 play pivotal roles in removing RNF168-induced ubiquitin conjugates from the BRCA1-A complex, without USP37 impairing DSB repair (22)."
PLAGL2 affects USP37
| 13 3
PLAGL2 increases the amount of USP37.
| 8
PLAGL2 increases the amount of USP37. 8 / 8
| 8

reach
"PLAGL2 activated the transcription of deubiquitinase USP37, which then interacted with and deubiquitinated Snail1 protein directly."

reach
"Finally, PLAGL2 modulates SNAI1 stability by activating USP37 transcription."
| PMC

reach
"Besides, we showed that PLAGL2 directly activates USP37 expression, which targets Snail1 to proteasome ubiquitination degradation."

reach
"Studies have shown that PLAGL2 can activate the transcription of the deubiquitinating enzyme USP37 that deubiquitinates and stabilizes Snail family transcriptional repressor 1 (Snail1), finally fostering Snail1-mediated cell proliferation [62]."

reach
"PLAGL2 modulates Snail1 stability by activating USP37 transcription."

reach
"These results collectively suggest that PLAGL2 transcriptionally activates USP37 expression to increase Snail1 protein levels, which in turn mediates the proliferation, migration, and invasion of GC cells."

reach
"Moreover, silencing PLAGL2 decreased the mRNA level of USP37 in SGC7901 cells, whereas PLAGL2 overexpression increased the USP37 mRNA level in AGS cells."

reach
"Wu et al. [25] also found that PLAGL2 could activate USP37 expression and stabilize Snail1 to promote the proliferation and migration of gastric cancer cells."
PLAGL2 binds USP37.
| 2 1
| 2 1

reach
"The ChIP assay revealed that PLAGL2 was bound to the USP37 promoter region."

reach
"PLAGL2 specifically binds to the GRGGC(N)6-8RGGK consensus sequences in the USP37 promoter region and activates its transcription, subsequently stabilizing SNAI1 and promoting EMT in GC cells [64]."
| PMC

sparser
"The ChIP assay revealed that PLAGL2 was bound to the USP37 promoter region (Figure xref C)."
PLAGL2 activates USP37.
| 1 2
PLAGL2 activates USP37. 3 / 3
| 1 2

sparser
"Although PLAGL2 does not function as a deubiquitinase to impede Snail1 ubiquitination, PLAGL2 transcriptionally activates USP37, which finally interacts with and deubiquitinates Snail1 directly."

sparser
"PLAGL2 can activate USP37, which in turn acts on Snail 1 to prevent its ubiquitination and promote gastric cancer cell proliferation and metastasis [ xref ]."

reach
"USP37, which is transcriptionally activated by PLAG1-like zinc finger 2, stabilizes Snail in a GSK-3β phosphorylation-dependent manner [59]."
PLAGL2 deubiquitinates USP37.
| 1
PLAGL2 leads to the deubiquitination of USP37. 1 / 1
| 1

reach
"PLAGL2 activated the transcription of deubiquitinase USP37, which then interacted with and deubiquitinated Snail1 protein directly."
PLAGL2 decreases the amount of USP37.
| 1
Modified PLAGL2 decreases the amount of USP37. 1 / 1
| 1

reach
"Moreover, silencing PLAGL2 decreased the mRNA level of USP37 in SGC7901 cells, whereas PLAGL2 overexpression increased the USP37 mRNA level in AGS cells."
BLM affects USP37
4 | 11
BLM binds USP37.
4 | 8
4 | 8

No evidence text available

reach
"Here, we demonstrate that the deubiquitinating enzyme USP37 interacts with BLM and that USP37 deubiquitinates and stabilizes BLM, thereby sustaining the DNA damage response (DDR)."

reach
"Mechanistically, DNA double-strand breaks (DSB) promotes ATM phosphorylation of USP37 and enhances the binding between USP37 and BLM."

reach
"Mechanistically, USP37 is phosphorylated by ATM following DNA damage, which increases the binding between USP37 and BLM and leads to BLM deubiquitylation."

reach
"We found that only HA-tagged USP37 bound with endogenous BLM (Figure 1A)."

reach
"In addition, we examined the endogenous interaction between USP37 and BLM in HEK293T cells and confirmed that USP37 interacted with BLM (Figure 1C, D)."

reach
"To support this notion, we found that the interaction between USP37 and BLM was enhanced upon cisplatin treatment in cells (Figure 4D)."

reach
"Collectively, these results indicate that DNA damage can strengthen the interaction between USP37 and BLM, which in turn stabilizes BLM."

reach
"We found that S114A mutation attenuated the interaction between USP37 and BLM (Figure 5E)."

No evidence text available
BLM activates USP37.
| 3
BLM activates USP37. 3 / 3
| 3

reach
"In addition, over-expression of BLM rescued DSB end resection in USP37 depletion cells (Supplementary Figure S4I, J)."

reach
"Moreover, we found that overexpressed BLM restored the cells resistance to cisplatin or IR in the USP37 knockdown cells reconstituted with USP37-C350A mutant (Supplementary Figure S6H–J)."

reach
"Furthermore, we found that overexpression of BLM rescued DSB end resection in USP37 depletion cells (Supplementary Figure S4I, J)."
USP37 affects MCM7
| 13 1
USP37 deubiquitinates MCM7.
| 12
USP37 deubiquitinates MCM7. 10 / 12
| 12

reach
"Although USP37 depletion enhanced this residual ubiquitylation of MCM7 (Fig. 4c, lane 5), most MCM7 remained unmodified, and importantly, the residual ubiquitylation was not dependent on TRAIP (Fig. 4c, lane 6)."

reach
"Proteomics and enzyme assays revealed USP37 interacts with the CMG complex to deubiquitinate MCM7, thus antagonizing replisome disassembly."

reach
"USP37 regulates the CMG complex by deubiquitinating MCM7."

reach
"To test if USP37 can deubiquitinate MCM7 in vitro, we mixed recombinant USP37 with our ubiquitinated protein eluate and assessed MCM7 deubiquitination over time by MCM7 immunoblotting."

reach
"This was dependent on USP37 activity, since USP37 harboring a C-S mutation of its active site cysteine at position 350 was unable to promote MCM7 deubiquitination (Fig. 4D compare lanes 7 and 8)."

reach
"Thus, USP37 can deubiquitinate MCM7, establishing that MCM7 ubiquitination is directly antagonized by USP37."

reach
"Collectively, these experiments demonstrate that USP37 can deubiquitinate MCM7 and suggest that it does so by preferentially removing long ubiquitin polymers."

reach
"Interestingly, in our hands, USP37 reduced but did not completely abolish ubiquitination of MCM7, suggesting that it may be removing a specific chain or chain type(s), rather than deubiquitinating the proximal ubiquitin conjugated directly onto MCM7 (Fig. 4D)."

reach
"Here, we demonstrate that premature CMG unloading is prevented by the USP37 deubiquitinase, which antagonizes MCM7 ubiquitination, thereby limiting aberrant unloading of the CMG helicase."

reach
"We show here that MCM7 ubiquitination is antagonized by USP37."
USP37 binds MCM7.
| 1
| 1

sparser
"To explore this hypothesis, we first established that FLAG-tagged USP37 can interact with V5 epitope-tagged MCM7 when expressed in HEK293T cells ( xref )."
USP37 activates MCM7.
| 1
USP37 activates MCM7. 1 / 1
| 1

reach
"Notably, USP37 overexpression increased MCM7 loading onto the insoluble nuclear fraction (P2; chromatin) of the chromatin fractionation ( Figure 5 C)."
USP37 affects CDKN1B
1 | 7 6
USP37 binds CDKN1B.
| 1 5
| 1 5

sparser
"Ectopically expressed USP37 formed a complex with p27, promoted its stabilization, blocked cell proliferation and induced the expression of REST-target neuronal differentiation genes."

sparser
"Interaction between USP37 and p27 was also confirmed by co-immunoprecipitation experiments using anti-p27 antibodies or control IgG followed by Western blot analysis using anti-Ub antibodies."

reach
"Interaction between USP37 and p27 was also confirmed by co-immunoprecipitation experiments using anti-p27 antibodies or control IgG followed by Western blot analysis using anti-Ub antibodies."

sparser
"USP37 formed a complex with p27, promoted its deubiquitination and stabilization, and blocked cell proliferation [ xref ]."

sparser
"Ectopically expressed wild type USP37 formed a complex with p27, promoted its deubiquitination and stabilization and blocked cell proliferation."

sparser
"In medulloblastomas, REST expression could decrease cyclin-dependent kinase (CDK)NIB/p27 (a CDK inhibitor) by repressing ubiquitin specific peptidase 37 (USP37), which could form a complex with p27 to promote its deubiquitination and stabilization, and resulting in blocked cell proliferation [ xref ]."
USP37 deubiquitinates CDKN1B.
1 | 2
USP37 leads to the deubiquitination of CDKN1B. 3 / 3
1 | 2

reach
"Constitutive expression of USP37 promotes p27 deubiquitination in medulloblastoma cells, whereas a catalytically dead USP37 mutant is unable to stabilize p27.Dobson et al. further explored the molecular basis of REST-induced USP37 downregulation and found that USP37 supresses medulloblastoma tumor growth in an orthotopic mouse model by modulating its downstream targets [87]."
| PMC

"Ectopically expressed wild-type <span class="match term0">USP37</span> formed a complex with <span class="match term1">p27</span>, promoted its deubiquitination and stabilization and blocked cell proliferation."

reach
"For example, USP37 promoted deubiquitination and stabilization of p27 and blocked medulloblastoma cell proliferation [ 18 ]."
USP37 ubiquitinates CDKN1B.
| 1 1
USP37 ubiquitinates CDKN1B. 2 / 2
| 1 1

sparser
"To confirm that USP37 ubiquitinated p27, we performed in vitro DUB assays using purified epitope tagged proteins from transiently transfected 293T cells."

reach
"To confirm that USP37 ubiquitinated p27, we performed in vitro DUB assays using purified epitope tagged proteins from transiently transfected 293T cells."
USP37 activates CDKN1B.
| 2
USP37 activates CDKN1B. 2 / 2
| 2

reach
"Since concomitant loss of REST and USP37 expression attenuated p27 stabilization and differentiation and rescued cell proliferation, our data strongly suggest that repression of USP37 and consequent p27 degradation, are important for REST dependent maintenance of cell proliferation."

reach
"Addition of USP37 to a reaction mix containing HA-ubiquitin and Myc-p27 caused a substantial increase in the 27kD form of p27 and a corresponding decrease in the slower migrating forms of the protein relative to reactions containing USP37 and a protease inhibitor N-ethyl maleimide (NEM) or USP37 C350-S or USP1 (XREF_FIG)."
USP37 inhibits CDKN1B.
| 1
USP37 inhibits CDKN1B. 1 / 1
| 1

reach
"Knockdown of REST and USP37 prevented p27 stabilization and blocked the diminution in proliferative potential that normally accompanied REST loss."
MiR-320b affects USP37
| 12
MiR-320b activates USP37.
| 5
MiR-320b activates USP37. 5 / 5
| 5

reach
"MiR-320b inhibits IH mediated lung cancer progression by targeting USP37."

reach
"These findings have confirmed that miR-320b inhibits IH mediated lung cancer progression by targeting USP37."

reach
"Taken together, our data suggested that miR-320b inhibited IH mediated lung cancer progression by targeting USP37."

reach
"The results revealed that 6IH treatment could decrease the expression of miR-320b yet increase that of USP37 and CDT1, and the overexpression of miR-320b could downregulate the expressions of USP37 and CDT1, while the effect of miR-320b mimic on the expressions of miR-320b, USP37, and CDT1 could be counteracted by overexpressed CDT1 (oe-CDT1)."

reach
"Taken together, our study suggested that miR-320b suppressed CDT1 by targeting USP37 to inhibit the progression of lung cancer."
MiR-320b inhibits USP37.
| 4
MiR-320b inhibits USP37. 4 / 4
| 4

reach
"Overexpression of miR-320b downregulates USP37 in an in vivo xenograft model, resulting in repression of the CDT1 inhibition of LC progression."
| PMC

reach
"Finally, the findings of our study concluded that miR-320b inhibited the tumorigenesis and progression of lung cancer by regulating the USP37 and CDT1 axis (XREF_FIG)."

reach
"We have also proven that miR-320b inhibited lung cancer tumorigenesis and progression by regulating USP37 and CDT1 axis, by using invivo models of human lung cancer xenograft."

reach
"However, co-transfecting miR-320b mimic and USP37 failed to inhibit IH induced lung cancer cell invasion (XREF_FIG D and XREF_SUPPLEMENTARY B), indicating that miR-320b inhibited 6IH induced invasion in a USP37 dependent manner."
MiR-320b decreases the amount of USP37.
| 3
MiR-320b decreases the amount of USP37. 3 / 3
| 3

reach
"We found that the expression of miR-320b was decreased while that of USP37 and CDT1 were increased in mice bearing IH treated cells, and miR-320b agmoir significantly increased the expression of miR-320b and reduced the expressions of USP37 and CDT1."

reach
"The overexpression of miR-320b significantly reduced the protein level of USP37 while inhibiting miR-320b resulted in an increased expression of USP37 (XREF_FIG H and 3I)."

reach
"The results revealed that 6IH treatment could decrease the expression of miR-320b yet increase that of USP37 and CDT1, and the overexpression of miR-320b could downregulate the expressions of USP37 and CDT1, while the effect of miR-320b mimic on the expressions of miR-320b, USP37, and CDT1 could be counteracted by overexpressed CDT1 (oe-CDT1)."
USP37 affects GLI1
1 | 9 2
USP37 binds GLI1.
1 | 4 2
1 | 4 2

reach
"Further studies detected that USP37 also interacted with and stabilized Gli-1 protein, which is the main activator of Hedgehog target gene."

sparser
"MG132, CHX chase, immunofluorescence staining and co-immunoprecipitation assays were used to test the interaction between USP37 and Gli-1."

sparser
"Co-IP assay indicated that USP37 interacted with Gli-1(Fig. xref )."

reach
"MG132, CHX chase, immunofluorescence staining and co-immunoprecipitation assays were used to test the interaction between USP37 and Gli-1."

reach
"USP37 also interacted with and stabilized glioma-associated oncogene 1 (Gli-1) protein."

reach
"USP37 interacts with and stabilizes Gli-1 protein."

No evidence text available
USP37 activates GLI1.
| 3
USP37 activates GLI1. 3 / 3
| 3

reach
"We found that lower expression level of USP37 obviously impaired the expression of Hh targets (Smo and Gli-1) and cell proliferation marker Ki-67 in the tumor tissues as seen by immunohistochemical staining (Fig. 8e)."

reach
"Furthermore, knockdown of USP37 expression significantly decreased the stability of Gli-1 protein both in MCF-7 and MDA-MB-231 cells (Fig. 7d and e)."

reach
"Knockdown of USP37 significantly decreased hedgehog (Hh) pathway components Smo and Gli-1."
USP37 increases the amount of GLI1.
| 1
USP37 increases the amount of GLI1. 1 / 1
| 1

reach
"Upregulation of USP37 could enhance protein expression levels of Smo and Gli-1."
USP37 deubiquitinates GLI1.
| 1
Modified USP37 leads to the deubiquitination of GLI1. 1 / 1
| 1

reach
"Moreover, overexpression of USP37 in breast cancer promoted stemness, cell invasion, and chemoresistance by deubiquitinating the glioma associated oncogene 1 protein XREF_BIBR."
USP37 affects CMG
| 12
USP37 inhibits CMG.
| 7
USP37 inhibits CMG. 7 / 7
| 7

reach
"Collectively, these results indicate that when forks experience stress in egg extracts and in mammalian cells, USP37 prevents CMG disassembly by TRAIP."

reach
"Strikingly, USP37 depletion enhanced CMG disassembly even when meDPCs were spaced ~1000 bp apart (Extended Data Fig. 6b, lanes 1–12)."

reach
"Since USP37 interaction with cohesin was reported previously , we tested whether immunodepletion of SMC3 phenocopies USP37 depletion in causing premature CMG unloading."

reach
"Notably, we have found that when replisome convergence was delayed by the TOP2 inhibitor ICRF-193, USP37 was required to suppress TRAIP-dependent premature CMG unloading."

reach
"Similarly, when we stalled forks with DNA-protein cross-links, USP37 prevented TRAIP-dependent CMG unloading."

reach
"Based on these observations, we propose that when converging forks stall, USP37 prevents CMG unloading by TRAIP (Fig. 6), and we speculate that a similar phenomenon occurs when mammalian cells are treated with topoisomerase inhibitors.We also found that TRAIP promotes CMG unloading in USP37-depleted extracts even when forks were stalled ~1 kb apart."

reach
"USP37 prevents CMG disassembly by TRAIP upon TOP2alpha inhibition."
USP37 deubiquitinates CMG.
| 3
USP37 leads to the deubiquitination of CMG. 3 / 3
| 3

reach
"Based on experiments in cell-free extracts and mammalian cells, we report here that under certain forms of replication stress, USP37 prevents CMG ubiquitylation and unloading by the TRAIP E3 ubiquitin ligase."

reach
"These data thus indicated that USP37 suppresses replisome ubiquitylation and disassembly by TRAIP when CMGs stall at close range for an extended time.To explore the distance-dependence of CMG ubiquitylation, we generated a panel of meDPC substrates in which DPCs were separated by 56, 305, and 1033 bp (Extended Data Fig. 6b, top)."

reach
"These results argue that when replisomes encounter trapped TOP2α complexes, USP37 suppresses CMG ubiquitylation and p97-dependent unloading.We next tested whether CMG unloading in the absence of USP37 depends on TRAIP."
USP37 binds CMG.
| 2
| 2

reach
"We therefore tested whether the USP37 PH domain is similarly important for USP37 binding to CMG."

reach
"Through a series of complementary approaches, we discovered that USP37 binds to the replisome, controls the level of polyubiquitin on MCM7 in cells, and prevents premature CMG unloading."
Snail1 affects USP37
| 12
USP37 binds Snail1. 10 / 12
| 12

reach
"USP37 interacts with and deubiquitinates Snail1 directly."

reach
"We further investigated the interaction between USP37 and Snail1."

reach
"Given that the SRD domain is essential for the interaction between USP37 and Snail1, we evaluated its effect on Snail1 ubiquitination in HEK293T cells."

reach
"Therefore, the SRD domain is also crucial for USP37 mediated Snail1 deubiquitination and the interaction between USP37 and Snail1."

reach
"Overall, these results demonstrate that USP37 interacts with and deubiquitinates Snail1 directly."

reach
"To explore whether the interaction between Snail1 and USP37 requires Snail1 phosphorylation, we utilized CIP to remove phosphate groups from the substrate proteins."

reach
"Strikingly, Co-IP experiments showed that the interaction between USP37 and Snail1 was strongly impeded by CIP intervention."

reach
"Since GSK-3beta is the major kinase involved in Snail1 phosphorylation in the SRD region, the GSK-3beta inhibitor LiCl was used to assess GSK-3beta inhibition in the interaction of USP37 and Snail1."

reach
"As shown in Figure XREF_FIG B, LiCl treatment also significantly disrupted the USP37 and Snail1 interaction."

reach
"Co-IP results showed that GSK-3beta siRNAs diminished approximately 60% of the interaction of USP37 and Snail1."
REST affects USP37
| 10 1
REST increases the amount of USP37.
| 3
REST increases the amount of USP37. 3 / 3
| 3

reach
"Regulation of USP37 Expression by REST associated G9a dependent Histone Methylation."

reach
"Notably, Das et al. reported that REST induces medulloblastoma oncogenesis by repressing USP37 transcription, thereby leading to low levels of the p27 tumor suppressor, which controls proliferation and cell cycle exit by inhibiting CDK1 in cerebellar progenitor cells."
| PMC

reach
"Like p27, a reciprocal association between USP37 and REST is present, and REST KD increases USP37 mRNA levels."
| PMC
REST decreases the amount of USP37.
| 3
REST decreases the amount of USP37. 3 / 3
| 3

reach
"REST also represses the transcription of the anti-proliferative deubiquitylase USP37, and drugs that reactivate USP37 expression may have therapeutic applications [XREF_BIBR]."

reach
"Conversely, REST knockdown upregulated USP37 gene expression and promoted an increase in p27 protein levels."

reach
"Previously, we observed that the expression of USP37 is transcriptionally repressed by the RE1 Silencing Transcription Factor (REST), which requires chromatin remodeling factors for its activity."
REST binds USP37.
| 1 1
| 1 1

sparser
"However, the association of USP37 with REST needs further investigation."

reach
"Like p27, a reciprocal association between USP37 and REST is present, and REST KD increases USP37 mRNA levels."
| PMC
REST activates USP37.
| 2
REST activates USP37. 2 / 2
| 2

reach
"Constitutive expression of USP37 promotes p27 deubiquitination in medulloblastoma cells, whereas a catalytically dead USP37 mutant is unable to stabilize p27.Dobson et al. further explored the molecular basis of REST-induced USP37 downregulation and found that USP37 supresses medulloblastoma tumor growth in an orthotopic mouse model by modulating its downstream targets [87]."
| PMC

reach
"In medulloblastomas, REST expression could decrease cyclin dependent kinase (CDK) NIB and p27 (a CDK inhibitor) by repressing ubiquitin specific peptidase 37 (USP37), which could form a complex with p27 to promote its deubiquitination and stabilization, and resulting in blocked cell proliferation [XREF_BIBR]."
REST inhibits USP37.
| 1
REST inhibits USP37. 1 / 1
| 1

reach
"Recently, REST repression of the gene encoding USP37, a protein deubiquitinase, has been proposed to play a key role in medulloblastoma generation."
USP37 affects SALL4
| 7 4
USP37 binds SALL4.
| 2 3
| 2 3

sparser
"Immunoprecipitates were eluted and then used for WB analysis to assess the interaction between USP37 and SALL4."

sparser
"The interaction between USP37 and SALL4 was confirmed by co-immunoprecipitation assay."

reach
"The interaction between USP37 and SALL4 was confirmed by co-immunoprecipitation assay."

sparser
"Besides, SALL4 level was enriched in anti-USP37, indicating that USP37 could bind to SALL4 protein (Fig.  xref H)."

reach
"The cell lysate was incubated with anti-IgG, anti-SALL4 and anti-USP37 for 2 h at room temperature, and then treated with protein A/G agarose (HY-K0230, MedChemExpress) for 1 h. Immunoprecipitates were eluted and then used for WB analysis to assess the interaction between USP37 and SALL4."
USP37 increases the amount of SALL4.
| 2
USP37 increases the amount of SALL4. 2 / 2
| 2

reach
"The results showed that only USP37 knockdown could reduce SALL4 expression."

reach
"By screening, we determined that USP37 knockdown could reduce SALL4 expression, and further analysis confirmed that USP37 could stabilize SALL4 expression by deubiquitinating."
USP37 deubiquitinates SALL4.
| 2
USP37 deubiquitinates SALL4. 2 / 2
| 2

reach
"Further analysis revealed that proteasome inhibitor MG132 reverted the reducing effect of si-USP37 on SALL4 protein expression (Fig. 4I), and knockdown of USP37 promoted the ubiquitination of SALL4 (Fig. 4J)."

reach
"The experimental results indicated that USP37 deubiquitinated SALL4 to stabilize its expression."
USP37 activates SALL4.
| 1 1
USP37 activates SALL4. 2 / 2
| 1 1

sparser
"USP37-activated SALL4 might enhance keloid fibroblast growth, invasion, migration, ECM accumulation and glycolysis via activating PI3K/AKT pathway."

reach
"USP37-activated SALL4 might enhance keloid fibroblast growth, invasion, migration, ECM accumulation and glycolysis via activating PI3K/AKT pathway."
ATM affects USP37
| 9 2
ATM phosphorylates USP37. 9 / 9
| 7 2

reach
"Mechanistically, DNA double-strand breaks (DSB) promotes ATM phosphorylation of USP37 and enhances the binding between USP37 and BLM."

reach
"Mechanistically, USP37 is phosphorylated by ATM following DNA damage, which increases the binding between USP37 and BLM and leads to BLM deubiquitylation."

reach
"These results confirm that ATM phosphorylates USP37 following DNA damage."

sparser
"Mechanistically, Chenming Wu et al. discovered that DNA double-strand breaks promote the phosphorylation of USP37 by ATM and enhance the binding between USP37 and the RecQ helicase BLM ( xref )."

reach
"Interestingly, WT USP37 was phosphorylated by ATM, which in turn increased its deubiquitination activity towards BLM, while the S114A mutant did not have this effect."

reach
"Together, these data suggest that USP37 phosphorylation by ATM is important for its regulation of DDR."

reach
"Mechanistically, DNA damage activates ATM which phosphorylates USP37 and activates its deubiquitinase activity to stabilize BLM."

sparser
"Following DNA damage, the DUB USP37 is phosphorylated by ATM."

reach
"Mechanistically, Chenming Wu et al. discovered that DNA double-strand breaks promote the phosphorylation of USP37 by ATM and enhance the binding between USP37 and the RecQ helicase BLM (111)."
ATM phosphorylates USP37 on S114. 2 / 2
| 2

reach
"We further confirmed that mutation of S114 (S114A) abrogated the phosphorylation following cisplatin treatment (Figure 5D), suggesting that USP37 is phosphorylated at S114 by ATM following DNA damage."

reach
"Collectively, these data suggest that S114 phosphorylation enhances the interaction between UPS37 and BLM and S114 phosphorylation of USP37 by ATM is important for USP37 deubiquitinating BLM following DNA damage."
Ubiquitin affects USP37
| 1 9
Ubiquitin binds USP37.
| 9

sparser
"One possibility is that phosphorylation enhances USP37 binding to its ubiquitinated substrates by altering the configuration of its three ubiquitin-interacting motifs (UIMs) spanning Ser 704 -Ser 723 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Detecting endogenous ubiquitin-protein conjugates that coimmunoprecipitate with transfected wild-type and UIM mutant USP37 proteins suggested only UIM2 and UIM3, rather than UIM1, permit the interaction between USP37 and ubiquitin-protein conjugates in a synergistic manner."
| PMC

sparser
"To determine whether the physical association between USP37 and WAPL is dependent on the ubiquitin-binding activity of USP37, the same mutations were introduced into full-length FLAG-USP37 and express[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"The deubiquitinase activity of USP37 and its ubiquitin interacting motifs are essential for the deubiquitination of SAMHD1, whereas the phosphorylation state of USP37 does not influence its activity."

sparser
"Because USP37 stabilizes chromatin-associated WAPL and the ubiquitin-binding activities of USP37 are important for its association with WAPL, we next aimed to determine whether USP37 regulates deubiqu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In the case of K48-linked substrates, binding of the UIMs to the proximal Ub increased catalytic efficiency of USP37 largely through k cat and to a lesser degree through K M . This result was somewhat counter-intuitive since we expected that binding of the UIMs of USP37 to Ub to have a more pronounced effect on K M (a proxy for binding affinity) since others have shown that UIM mutations perturbed the ability to pull down Ub conjugates xref ."

sparser
"Mutations in UIM2 and/or UIM3 perturb USP37 binding to endogenous ubiquitin–protein conjugates [ xref , xref , xref ]."

sparser
"The issue of how the binding of the UIMs of USP37 to the proximal Ub of K48 di-Ub enhances activity remains an open question."

sparser
"We expected that, if USP37 deubiquitylates WAPL, the ubiquitin-binding activities of USP37 would be critical for the interaction between USP37 and WAPL."
Ubiquitin activates USP37.
| 1
| 1

reach
"Since, USP37 is targeted by both these ubiquitin ligases and HBx chaperoned USP37 out of the nucleus, we wondered if it was safeguard mechanism to prevent the ubiquitination and degradation of USP37."
USP37 affects USP37
| 10
USP37 activates USP37.
| 4
USP37 activates USP37. 4 / 4
| 4

reach
"Mechanistically, CDK2 phosphorylates USP37 at serine 628 (Ser ) and then activates USP37 deubiquitinase activity."

reach
"Silencing of USP37 expression induced MET including upregulated E-cadherin expression and downregulated N-cadherin expression, while PM reversed MET in silenced USP37 cells."

reach
"Ambiguously, the transcription of USP37 is suppressed in medulloblastoma cells through the activity of RE1 silencing transcription factor to prevent the USP37 mediated stabilization of the cyclin dependent kinase inhibitor p27, which is known to act as a negative regulator of cell cycle XREF_BIBR."

reach
"To further investigate whether USP37 phosphorylation is able to activate USP37, a two-step assay was carried out."
USP37 increases the amount of USP37.
| 3
USP37 increases the amount of USP37. 3 / 3
| 3

reach
"Besides, the overexpression of CI USP37 significantly increased the ubiquitination level of USP37 (XREF_FIG N)."

reach
"After a correlation of OS and DFS was seen with the expression of USP37, we modulated the expression of USP37 by overexpression and depletion of USP37 to identify its molecular targets in osteosarcoma especially pertaining to replication stress."

reach
"The results showed reduced survival of U2OS cells in colony formation assays after USP37 inhibition and exposure to HU-mediated replication stress, while overexpression of USP37 led to enhanced survival in response to HU (Fig. 4A), suggesting that USP37 plays a crucial role in aiding the survival of osteosarcoma cells under replication stress.Exposure to replication stress has been shown to affect the spatial localization and levels of USP37 and its associated interacting partners."
USP37 decreases the amount of USP37.
| 2
USP37 decreases the amount of USP37. 2 / 2
| 2

reach
"Premature CMG unloading was suppressed by supplementing USP37-depleted extracts with wild-type HA-tagged USP37 (USP37 ; expressed in a transcription-translation extract), but not with catalytically inactive USP37 (USP37 ; Fig. 3b, lanes 2, 4, 6, 8; Extended Data Fig. 5f)."

reach
"After a correlation of OS and DFS was seen with the expression of USP37, we modulated the expression of USP37 by overexpression and depletion of USP37 to identify its molecular targets in osteosarcoma especially pertaining to replication stress."
USP37 deubiquitinates USP37.
| 1
Ubiquitinated USP37 leads to the deubiquitination of USP37. 1 / 1
| 1

reach
"The level of USP37 ubiquitination was also reduced by mutations in UIM2 or UIM3, suggesting their role in ubiquitination of USP37 itself."
USP37 affects Cohesin
| 4 5
USP37 binds Cohesin.
| 3 5
| 3 3

reach
"Since USP37 interaction with cohesin was reported previously , we tested whether immunodepletion of SMC3 phenocopies USP37 depletion in causing premature CMG unloading."

reach
"This discrepancy may be due to cell type specific or technical differences, since the reported interaction between USP37 and cohesin required ectopic expression of at least one component of the putative complex."

sparser
"This discrepancy may be due to cell type–specific or technical differences, since the reported interaction between USP37 and cohesin required ectopic expression of at least one component of the putative complex."

sparser
"Indeed, cohesin subunits MYC-SMC1, MYC-SCC1, MYC-SA2, and GFP-SMC3 co-immunoprecipitate with FLAG-USP37 in HEK293 cells ( Figure 2 C), confirming that USP37 associates with cohesin."

reach
"Given the mitotic delay and chromosome segregation errors we observed in USP37 depleted cells and that our study and others have implicated cohesion factors in regulating these processes [17, 18], we [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Since USP37 interaction with cohesin was reported previously xref , we tested whether immunodepletion of SMC3 phenocopies USP37 depletion in causing premature CMG unloading."
| 1

sparser
"The authors further demonstrated that USP37 interacts with both cohesin and a negative regulator WAPL, which is required for releasing cohesin from chromatid arms in prophase."
| 1

sparser
"Identifying USP37-interacting partners with proximity-dependent biotin identification (BioID) found that USP37 interacts with the cohesin complex proteins SMC3, SMC1, SSCC1, and SA1/2, as well with the regulators of cohesion WAPL and NIPBL [ xref ]."
| PMC
USP37 activates Cohesin.
| 1
USP37 activates Cohesin. 1 / 1
| 1

reach
"Given the critical role of WAPL in the prophase pathway, it will be interesting to discern whether USP37 contributes to other WAPL dependent processes, such as chromatin structure or protecting cohesi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP37 affects CHEK1
3 | 6 1
USP37 binds CHEK1.
3 | 1 1
3 | 1 1

No evidence text available

No evidence text available

sparser
"Given that we have previously demonstrated USP37's interaction with CHK1 its ability to enhance CHK1 activity [ xref ], both of which are closely associated with replication fork movement, and that USP37 is also known to play a crucial role in the Homologous Recombination pathway [ xref ], we conducted a DNA fiber assay to evaluate replication fork movement at the single molecule level."

No evidence text available

reach
"We did observe deubiquitination of CHK1 in the same assay, consistent with earlier reports.Given that we have previously demonstrated USP37's interaction with CHK1 its ability to enhance CHK1 activity [23], both of which are closely associated with replication fork movement, and that USP37 is also known to play a crucial role in the Homologous Recombination pathway [55], we conducted a DNA fiber assay to evaluate replication fork movement at the single molecule level."
USP37 activates CHEK1.
| 3
USP37 activates CHEK1. 3 / 3
| 3

reach
"The deubiquitinating enzyme USP37 enhances CHK1 activity to promote the cellular response to replication stress."

reach
"Recently we demonstrated that USP37 enhances CHK1 activity to promote the cellular response to replication stress [23]."

reach
"In a recent study, we demonstrated that USP37 enhances CHK1 activity to promote cellular survival in response to replication stress, and its depletion leads to reduced survival [23]."
USP37 deubiquitinates CHEK1.
| 2
USP37 deubiquitinates CHEK1. 2 / 2
| 2

reach
"We further demonstrated that USP37 deubiquitinates CHK1, promoting its stability."

reach
"Subsequent investigations revealed that USP37 is responsible for the deubiquitination of CHK1, a protein whose destabilization occurs in the absence of USP37."
USP37 affects BRCA1
1 | 9
USP37 inhibits BRCA1.
| 5
USP37 inhibits BRCA1. 5 / 5
| 5

reach
"In agreement with this hypothesis, we found that USP26 or USP37 depletion inhibits the efficient association of BRCA1 with PALB2, a unique subunit of the BRCC complex."

reach
"USP26 and USP37 limit the ubiquitin-dependent sequestration of BRCA1 and facilitate BRCA1-PALB2-BRCA2-RAD51 association at damaged chromatin to promote HR repair [77]."

reach
"USP26 and USP37 have additionally been shown to impair accumulation of RAP80 and BRCA1 [154]."

reach
"Together, these results suggest that USP26 and USP37 promote the BRCA1 dependent loading of PALB2 and RAD51 by counteracting the repressive impact of RAP80 dependent BRCA1 sequestration and RAP80 dependent inhibition of end-resection during HR."

reach
"We reasoned that USP26 and USP37 may antagonize the RAP80 dependent sequestration of BRCA1 by removing RNF8 and RNF168 mediated ubiquitylation and thereby promote HR."
USP37 activates BRCA1.
| 4
USP37 activates BRCA1. 4 / 4
| 4

reach
"On the other hand, we also found that USP26 and USP37 promote the efficient association of BRCA1 with PALB2."

reach
"In relation to its role maintaining chromosomal integrity, USP37 localizes to double-strand breaks and promotes BRCA1 inclusion into complexes responsible for homologous recombination 16."

reach
"Loss of USP26 or USP37 impairs HR by counteracting RAP80 dependent sequestration of BRCA1."

reach
"Together, these results suggest that USP26 and USP37 promote the BRCA1 dependent loading of PALB2 and RAD51 by counteracting the repressive impact of RAP80 dependent BRCA1 sequestration and RAP80 dependent inhibition of end-resection during HR."
USP37 binds BRCA1.
1 |
1 |

No evidence text available
YWHAG affects USP37
3 1 |
3 1 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
3 2 | 1 1
USP37 binds.
2 |
2 |

No evidence text available

No evidence text available
USP37 is active.
3 |
USP37 phosphorylated on S628 is active. 3 / 3
3 |

"There is positive reinforcement of this signaling mechanism because phosphorylation of Ser628 by CDK2/cyclin E and CDK2/cyclin A complexes produces maximal USP37 activity"

"There is positive reinforcement of this signaling mechanism because phosphorylation of Ser628 by CDK2/cyclin E and CDK2/cyclin A complexes produces maximal USP37 activity"

"There is positive reinforcement of this signaling mechanism because phosphorylation of Ser628 by CDK2/cyclin E and CDK2/cyclin A complexes produces maximal USP37 activity"
USP37 translocates.
| 1 1
USP37 translocates from the nucleoplasm to the cytoplasm. 1 / 1
| 1

sparser
"A recent study demonstrated that HBx, an oncoprotein of the hepatitis B virus and a regulator of hepatocarcinogenesis, promoted the translocation of USP37 from the nucleoplasm to the cytoplasm and that the degradation of USP37 seemed to be prevented by the E3 ligases, APC/CDH1 and SCF/β-TrCP [ xref ]."
USP37 translocates to the replication fork. 1 / 1
| 1

reach
"Our study has found that USP37 interacts with PCNA, a central replication factor, and has a higher affinity for PCNA-DNA complex than for PCNA alone.We propose a model where USP37 is recruited to the replication fork by interacting with PCNA at high or physiological levels of USP37."
USP37 affects cisplatin
| 2 7
USP37 inhibits cisplatin.
| 2 3
| 2 3

reach
"USP37 knockdown inhibits tumorigenicity and increases sensitivity to cisplatin in vivo."

reach
"USP37 can regulate the stemness, cell invasion and EMT via Hh pathway, and decreased USP37 confers sensitivity to cisplatin in breast cancer cells."

reach
"Additionally, USP37 knockdown enhanced the sensitivity of breast cancer cells to cisplatin in vitro and in vivo."

eidos
"Additionally , USP37 knockdown enhanced the sensitivity of breast cancer cells to cisplatin in vitro and in vivo ."

eidos
"These results indicated that USP37 downregulation attenuates breast cancer progression and enhances sensitivity to cisplatin in vivo ."
USP37 activates cisplatin.
| 4
| 4

reach
"USP37 overexpression also increased the formation of spheroid and chemo-resistance to cisplatin-induced growth inhibition (Fig. 5b, d)."

reach
"Additionally, USP37 knockdown inhibited tumorigenicity and increased anticancer effect of cisplatin in vivo."

reach
"These results indicated that USP37 downregulation attenuates breast cancer progression and enhances sensitivity to cisplatin in vivo."

reach
"Finally, we demonstrated that high level of USP37 mediated cisplatin or IR resistance in breast cancer in a BLM-dependent manner and clarified the role of USP37 as a potential therapeutic target in breast cancer."
| 9
| 6

reach
"Accumulating evidence has demonstrated that USP37 promotes lung cancer cell proliferation, migration, and invasion XREF_BIBR but inhibits lung cancer cell apoptosis in a deubiquitination dependent manner."

reach
"USP37 up-regulation promoted the proliferation, cell cycle progression, apoptosis inhibition, migration, invasion, epithelial mesenchymal transition (EMT) and stemness of CRC cells; moreover, USP37 facilitated the angiogenesis of human umbilical vein endothelial cells (HUVECs)."

reach
"The results showed that USP37 knockdown significantly enhanced Sorafenib-triggered apoptosis in Huh7, SMMC-7721 and HepG2 cell lines (Fig. 4a and Figure S3 b,c)."

reach
"Depleting USP37 in osteosarcoma cells results in reduced recruitment to the replication fork, leading to instability of binding proteins, stalled replication fork, and enhanced apoptosis (Fig. 10B)."

reach
"USP37 knockdown repressed viability, EdU positive cell rate, promoted apoptosis rate in keloid fibroblasts, but these effects were reverted by SALL4 upregulation (Fig. 5B-D)."

reach
"The combined approach of USP37 knockdown and Dox treatment significantly increases cleaved Caspase 3 and Bax levels while suppressing BCL2 expression, resulting in cell cycle arrest and enhanced apoptosis [86]."
| 3

reach
"Our findings clearly show low levels of USP37 in NSCLC cells, which has synergistic effects on gefitinib-mediated ubiquitination and apoptotic cell death (Figs."

reach
"Our data indicated that USP37#2 shRNA combined with cisplatin induced the cell apoptosis with an underlying decrease in the Bcl-2/Bax ratio."

reach
"We detected that USP37#2 shRNA combined with cisplatin treatment induced cell apoptosis with an underlying decrease in Bcl-2/Bax ratio."
USP37 affects YWHAG
3 1 |
3 1 |

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USP37 affects Cyclin
| 9
USP37 deubiquitinates Cyclin.
| 4
USP37 deubiquitinates Cyclin. 4 / 4
| 4

reach
"These results argue that USP37 deubiquitinates cyclin A directly as opposed to being an EMI1-type APC/C inhibitor."

reach
"USP37 deubiquitinates and stabilizes Cyclin A and promotes S phase entry."

reach
"Usp37 binds APC/C Cdh1 in G1 and deubiquitinates the APC/C substrate cyclin A [XREF_BIBR]."

reach
"Deubiquitination and stabilization of the APC/C substrate cyclin A by USP37 enables entry into S phase."
USP37 activates Cyclin.
| 4
Mutated USP37 activates Cyclin. 2 / 2
| 2

reach
"USP37-FLAG, but not catalytic mutant USP37 C 350 S (hereafter called USP37DD for DUB-Dead), also increased endogenous cyclin A."

reach
"Reconstitution of an shRNA resistant KEN box-3 mutant USP37 in USP37-knockdown U2OS cells enables cyclin A to be accumulated again."
USP37 activates Cyclin. 2 / 2
| 2

reach
"In this regard, cyclin A2 degradation during interphase is specifically impeded by the deubiquitinating enzyme USP37 54, but this enzyme does not appear to antagonize cyclin B1 degradation."

reach
"USP37 was shown to induce the stability of cyclin A by deubiquitination, resulting in an accumulation of cyclin A, which leads to the G1-S phase transition [XREF_BIBR]."
USP37 inhibits Cyclin.
| 1
USP37 inhibits ubiquitinated Cyclin. 1 / 1
| 1

reach
"Western blot analysis of cyclin A following immunoprecipitation of HA-ubiquitin-modified proteins from SDS and heat denatured lysates revealed that USP37, but not USP37DD, reduced the amount of ubiqui[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP37 affects APC_C
| 8 1
USP37 binds APC_C.
| 5 1
| 5

reach
"Ubiquitin specific protease (USP) 44 has been described in checkpoint responsive regulation of the APC/C by stabilizing the APC-inhibitory MAD2 and CDC20 complex (Joo et al., 2007; Stegmeier et al., 2[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The core components of APC bind with USP37 through CDH1."
| PMC

reach
"EMI1 was not detected in the USP37 and APC/C complex (data not shown) and, similarly, USP37 was not present in the EMI1 and APC/C complex (M.K.S. and P.K.J., unpublished data)."

reach
"Early work showed that USP37 binds the APC/C CDH1 E3 complex to regulate ubiquitylation of cyclin A, a substrate of APC/C CDH1 [ 28 ]."

reach
"Knockdown of CDH1 markedly reduced the interaction between USP37 and CDC27, suggesting that core APC/C components bind USP37 indirectly through CDH1."
| 1

sparser
"Finally, specificity for Cdh1 may introduce possibilities for modulating the degradation of substrates via chain editing – for example, the DUB USP37 interacts with APC/CCdh1 to modulate K11 linkages on at least one substrate ( xref )."
USP37 activates APC_C.
| 2
USP37 activates APC_C. 2 / 2
| 2

reach
"Other APC CDH1 substrates, including CDC20, CDC6, PLK1, Aurora kinase A, and SKP2, were not decreased by USP37 knockdown, indicating a specific effect of USP37 on cyclin A abundance.Coimmunoprecipitat[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Moreover, USP37 contributes to the stabilization of cyclin A by counteracting APC/Cdh1 functioning."
USP37 inhibits APC_C.
| 1
USP37 inhibits APC_C. 1 / 1
| 1

reach
"Thus, Usp44 and Usp37 can restrict the activity of the major K11 specific ligase, the APC/C, in addition to deubiquitinating substrates modified with K11 linked chains."
NFE2L2 affects USP37
| 3 6
| 3 6

reach
"To gain further insight into the mechanism underlying NRF2 protein stability regulation by USP37, we conducted experiments to examine the interaction between USP37 and NRF2."

reach
"The results demonstrated reciprocal interactions between USP37 and NRF2 (Fig. 3a, b)."

reach
"The interaction between USP37 and NRF2 facilitates the removal of ubiquitin chains from NRF2, thereby stabilizing the NRF2 protein."

sparser
"Overall, our findings manifested the significant involvement of the USP37-NRF2 axis in regulating therapeutic interventions for HCC."

sparser
"The interaction between USP37 and NRF2 facilitates the removal of ubiquitin chains from NRF2, thereby stabilizing the NRF2 protein."

sparser
"USP37 interacts with NRF2 and enhances NRF2 deubiquitination."

sparser
"To gain further insight into the mechanism underlying NRF2 protein stability regulation by USP37, we conducted experiments to examine the interaction between USP37 and NRF2."

sparser
"The results demonstrated reciprocal interactions between USP37 and NRF2 (Fig.  xref a, b)."

sparser
"Additionally, USP37 interacts with NRF2 and facilitates its deubiquitination."
ERK affects USP37
| 4 5
| 4 5

sparser
"As shown in xref , the N-terminal region of USP37 (1–330 aa) was critical for the interaction between USP37 and ERK1/2."

sparser
"To investigate the biological function of the USP37-ERK1/2 axis in lung cancer cells in vivo, shRNAs were stably transfected into NCI-A549 cells, which were subcutaneously implanted into nude mice and monitored for tumor growth."

reach
"A coimmunoprecipitation assay was then performed to confirm the interaction between endogenous USP37 and ERK1/2."

reach
"The interaction between USP37 and ERK1/2 was further confirmed using a reciprocal coimmunoprecipitation assay (Fig. 3 D)."

reach
"In addition, the binding between USP37 and ERK1/2 was further confirmed using bacterially expressed GST-USP37 proteins (Fig. 3 E)."

reach
"As shown in Fig. 3 F, the N-terminal region of USP37 (1–330 aa) was critical for the interaction between USP37 and ERK1/2."

sparser
"USP37 interacts with ERK1/2."

sparser
"A coimmunoprecipitation assay was then performed to confirm the interaction between endogenous USP37 and ERK1/2."

sparser
"The interaction between USP37 and ERK1/2 was further confirmed using a reciprocal coimmunoprecipitation assay ( xref )."
Cohesin affects USP37
| 3 5
| 3 3

reach
"Since USP37 interaction with cohesin was reported previously , we tested whether immunodepletion of SMC3 phenocopies USP37 depletion in causing premature CMG unloading."

reach
"This discrepancy may be due to cell type specific or technical differences, since the reported interaction between USP37 and cohesin required ectopic expression of at least one component of the putative complex."

sparser
"This discrepancy may be due to cell type–specific or technical differences, since the reported interaction between USP37 and cohesin required ectopic expression of at least one component of the putative complex."

sparser
"Indeed, cohesin subunits MYC-SMC1, MYC-SCC1, MYC-SA2, and GFP-SMC3 co-immunoprecipitate with FLAG-USP37 in HEK293 cells ( Figure 2 C), confirming that USP37 associates with cohesin."

reach
"Given the mitotic delay and chromosome segregation errors we observed in USP37 depleted cells and that our study and others have implicated cohesion factors in regulating these processes [17, 18], we [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Since USP37 interaction with cohesin was reported previously xref , we tested whether immunodepletion of SMC3 phenocopies USP37 depletion in causing premature CMG unloading."
| 1

sparser
"The authors further demonstrated that USP37 interacts with both cohesin and a negative regulator WAPL, which is required for releasing cohesin from chromatid arms in prophase."
| 1

sparser
"Identifying USP37-interacting partners with proximity-dependent biotin identification (BioID) found that USP37 interacts with the cohesin complex proteins SMC3, SMC1, SSCC1, and SA1/2, as well with the regulators of cohesion WAPL and NIPBL [ xref ]."
| PMC
CDT1 affects USP37
3 | 2 3
3 | 2 3

No evidence text available

reach
"USP37 interacted with and deubiquitinated CDT1 to protect it from proteasomal degradation."

sparser
"Therefore, we further examined the 6 enriched genes in this pathway using an online tool (Chipbase) and found that USP37 and CDT1 were closely associated with lung cancer ( xref D)."

sparser
"In order to confirm this hypothesis, we performed a co-immunoprecipitation assay followed by an immunoblotting analysis, detecting CDT1 and USP37, respectively, in order to evaluate the interactions between USP37 and CDT1."

No evidence text available

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reach
"In order to confirm this hypothesis, we performed a co-immunoprecipitation assay followed by an immunoblotting analysis, detecting CDT1 and USP37, respectively, in order to evaluate the interactions between USP37 and CDT1."

sparser
"Previous studies have demonstrated that USP37 interacts with CDT1, a key protein in replication fork progression, and stabilizes its phosphorylated form [ xref ]."
BTRC affects USP37
7 1 | 1
BTRC binds USP37.
7 | 1
7 | 1

No evidence text available

reach
"HBx acts as a chaperone of USP37 and shuttles it out of the nucleus, where the ubiquitin E3 ligase CDC20 homolog 1 (CDH1) and b-TrCP associate with USP37 (Zhou et al., 2003; von Mikecz, 2006; Saxena and Kumar, 2014)."

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BTRC ubiquitinates USP37.
1 |
BTRC ubiquitinates USP37. 1 / 1
1 |

"Skp1-Cul1-F-box ubiquitin ligase (SCF(<span class="match term0">betaTrCP</span>))-mediated destruction of the ubiquitin-specific protease <span class="match term1">USP37</span> during G2-phase promotes mitotic entry"
APC_C affects USP37
| 8 1
APC_C binds USP37.
| 5 1
| 5

reach
"Ubiquitin specific protease (USP) 44 has been described in checkpoint responsive regulation of the APC/C by stabilizing the APC-inhibitory MAD2 and CDC20 complex (Joo et al., 2007; Stegmeier et al., 2[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The core components of APC bind with USP37 through CDH1."
| PMC

reach
"EMI1 was not detected in the USP37 and APC/C complex (data not shown) and, similarly, USP37 was not present in the EMI1 and APC/C complex (M.K.S. and P.K.J., unpublished data)."

reach
"Early work showed that USP37 binds the APC/C CDH1 E3 complex to regulate ubiquitylation of cyclin A, a substrate of APC/C CDH1 [ 28 ]."

reach
"Knockdown of CDH1 markedly reduced the interaction between USP37 and CDC27, suggesting that core APC/C components bind USP37 indirectly through CDH1."
| 1

sparser
"Finally, specificity for Cdh1 may introduce possibilities for modulating the degradation of substrates via chain editing – for example, the DUB USP37 interacts with APC/CCdh1 to modulate K11 linkages on at least one substrate ( xref )."
APC_C inhibits USP37.
| 2
APC_C inhibits USP37. 2 / 2
| 2

reach
"Other APC CDH1 substrates, including CDC20, CDC6, PLK1, Aurora kinase A, and SKP2, were not decreased by USP37 knockdown, indicating a specific effect of USP37 on cyclin A abundance.Coimmunoprecipitat[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"USP37 antagonises APC/C to stabilise Cyclin A at S-phase entry (Huang et al, 2011), whilst OTUD7B (Cezanne) exhibits K11-linkage specificity (Mevissen et al, 2013) and deubiquitinates both Cyclin B1 and Aurora A (Bonacci et al, 2018)."
APC_C activates USP37.
| 1
APC_C activates USP37. 1 / 1
| 1

reach
"The inactivation of APC and C-CDH 1 is reinforced through the ubiquitin dependent degradation of CDH1 in S phase by the SKP1-Cullin-F-box (SCF) family ubiquitin ligase, SCF-cyclin F, as well as through deubiquitination of the critical APC and C-CDH 1 target cyclin A2 in late G1 and S phases by the E2F induced deubiquitinase USP37."

reach
"Loss of USP26 or USP37 impairs HR by counteracting RAP80 dependent sequestration of BRCA1."

reach
"BRCC36, USP13, USP26, USP37 and USP48 all participate in regulating BRCA1-mediated HR, but they seemly have little effect on the stability of BRCA1 ."

reach
"Given that USP26 and USP37 are able to reverse RNF8/168 mediated ubiquitylation and promote HR, these enzymes would be ideal candidates to facilitate the repositioning of ubiquitylated substrates away from the DSB."

reach
"A striking conclusion from our study is that both over-expression as well as knockdown of USP26 or USP37 impairs HR."

reach
"Interestingly, in contrast with initial findings assessing overexpression of these DUBs, knockdown of either USP26 or USP37 impairs accumulation of key HR factors including RAD51 as well as PALB2, CtIP, and RPA [154]."

reach
"Loss of USP26 and USP37 function markedly impairs the assembly of PALB2, RAD51 and efficient HR."

reach
"Depletion of USP26 or USP37 disrupts the execution of HR and this effect is alleviated by the simultaneous depletion of RAP80."

reach
"We reasoned that USP26 and USP37 may antagonize the RAP80 dependent sequestration of BRCA1 by removing RNF8 and RNF168 mediated ubiquitylation and thereby promote HR."
| 8
USP37 activates cell migration.
| 6

reach
"USP37 Promotes Lung Cancer Cell Migration by Stabilizing Snail Protein"

reach
"Moreover, we found that knockdown of USP37 suppressed cell migration and invasion in breast cancer cells."

reach
"USP37 knockdown suppresses breast cancer cell migration and invasion by promoting mesenchymal-epithelial transition."

reach
"Overexpression of wild-type USP37, but not its catalytically inactive mutant C350S, promotes cancer cell migration."

reach
"Knockdown of USP37 evidently inhibited cell migration capacity in both MCF and MDA-MB-231 cells (Fig. 3f, g)."

reach
"Besides, USP37 deubiquitination-stabilized snails promoted lung cancer cell migration ."
USP37 inhibits cell migration.
| 2

reach
"Indeed, USP37 KD reduces cell migration in transwell assays [62]."
| PMC

reach
"Importantly, depletion of USP37 downregulates endogenous SNAI1 protein and suppresses cell migration, which can be reversed by re-expression of SNAI1."
USP37 affects UBC
8 |
8 |

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USP37 affects FZR1
2 6 |
USP37 binds FZR1.
1 6 |
1 6 |

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"Here we show that USP37 binds the APC/C coactivator CDH1|Deubiquitinase USP37 is activated by CDK2 to antagonize APC(CDH1) and promote S phase entry"
USP37 inhibits FZR1.
1 |
USP37 inhibits FZR1. 1 / 1
1 |

"Here we show that USP37 binds the APC/C coactivator CDH1|Deubiquitinase USP37 is activated by CDK2 to antagonize APC(CDH1) and promote S phase entry"
USP37 affects FBXW11
7 | 1
7 |

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| 1

sparser
"Consistent with this hypothesis, USP37 interacts with components of the SCF in a βTrCP-dependent manner."
USP37 affects BTRC
7 | 1
7 | 1

No evidence text available

reach
"HBx acts as a chaperone of USP37 and shuttles it out of the nucleus, where the ubiquitin E3 ligase CDC20 homolog 1 (CDH1) and b-TrCP associate with USP37 (Zhou et al., 2003; von Mikecz, 2006; Saxena and Kumar, 2014)."

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UBC affects USP37
8 |
8 |

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FBXW11 affects USP37
7 | 1
7 |

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| 1

sparser
"Consistent with this hypothesis, USP37 interacts with components of the SCF in a βTrCP-dependent manner."
E2F1 affects USP37
7 | 1
E2F1 decreases the amount of USP37.
7 |
E2F1 decreases the amount of USP37. 7 / 7
7 |

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E2F1 increases the amount of USP37.
| 1
Mutated E2F1 increases the amount of USP37. 1 / 1
| 1

reach
"Not surprisingly, transactivation mutant of E2F1, E2F1 1-374 (DeltaC) is unable to stimulate the expression of USP37 gene."
USP37 affects ZBTB16
| 5 1
USP37 binds ZBTB16.
| 2 1
| 2 1

reach
"We showed that USP37 interacted with PLZF and RARA through the PLZF moiety and sustained PLZF and RARA steady state levels."

reach
"In contrast, the deubiquitinating enzyme USP37 interacts with Plzf which increases Plzf protein stability [XREF_BIBR]."

sparser
"In contrast, the deubiquitinating enzyme USP37 interacts with Plzf which increases Plzf protein stability [ xref ]."
USP37 activates ZBTB16.
| 3
USP37 activates ZBTB16. 3 / 3
| 3

reach
"Subsequently, it was verified that USP37 expression modulated the oncogenic fusion protein PLZF/RARA stability and cell transformation potential in PLZF/RARA-associated acute promyelocytic leukemia [33]."

reach
"It was reported that USP37 induced the stability of the oncogenic fusion protein PLZF and RARA."

reach
"Furthermore, overexpression or depletion of USP37 caused an increase or decrease of PLZF and RARA protein half-life, correlating with down- or upregulation of PLZF and RARA poly-ubiquitination, respectively."
USP37 affects X
| 7
USP37 binds X. 7 / 7
| 7

reach
"Our co-immunoprecipitation and confocal microscopic studies suggested a direct interaction between USP37 and HBx."

reach
"Further, we observed that HBx interacted with USP37 and chaperoned it out of nucleus to prevent its ubiquitination and degradation by E3 ubiquitin ligases."

reach
"The dichotomy in the action of two E3 ligases targeting USP37 further inspired us to investigate the possibility of direct or indirect interaction between HBx and USP37 which could reveal a bigger role of HBx in protection of USP37."

reach
"HBx interacts with USP37 and translocates it to cytoplasm."

reach
"Since, HBx and USP37 co-localized in cells, we next examined the possibility of a physical interaction between HBx and USP37."

reach
"Venturing further we explored the possibility of a physical interaction between HBx and USP37."

reach
"This study has relied on the cell culture based system where HBx was co-expressed along with USP37-DD or USP37 either in immortalized human hepatocytes or in hepatoma Huh7 cells to elucidate the oncogenic cooperation between HBx and USP37."
USP37 affects S phase
| 7
USP37 activates S phase.
| 5
USP37 activates S phase. 4 / 4
| 4

reach
"Importantly, USP37 expression rescued chromatin-bound CDC45 in S phase, indicating a direct and specific role for USP37 in preventing replisome disassembly (Fig. 2D and E)."

reach
"USP37 was also reported to remove ubiquitin chains from Cyclin A and overexpression of USP37 promotes Cyclin A accumulation and accelerated S phase entry, suggesting a potentially key role for USP37 in regulating the cell cycle in a cancer setting (73)."

reach
"The deubiquitinase (DUB) USP37 has been found to stabilize cyclin A by removing the polyubiquitin from the latter and, hence, accelerates entry into S phase (Huang et al., 2011)."

reach
"USP37 promotes S phase entry by activating CDK2 and regulates cell proliferation by stabilizing p27 ( Huang et al., 2011 ; Das et al., 2013 ), and USP39 inhibits malignant proliferation of several tum[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP37 bound to CDH1 activates S phase. 1 / 1
| 1

reach
"USP37 binds to CDH1 and removes degradative polyubiquitin chains, leading to the early accumulation of cyclin A in the G1 phase and accelerated entry into the S phase (18)."
USP37 inhibits S phase.
| 2
USP37 bound to G1 and Cyclin_A inhibits S phase. 1 / 1
| 1

reach
"USP37 binds to CDH1 and removes degradative polyubiquitin chains, leading to the early accumulation of cyclin A in the G1 phase and accelerated entry into the S phase (18)."
USP37 bound to polyubiquitin chains inhibits S phase. 1 / 1
| 1

reach
"USP37 binds to CDH1 and removes degradative polyubiquitin chains, leading to the early accumulation of cyclin A in the G1 phase and accelerated entry into the S phase (18)."
USP37 affects ESR1
3 | 1 3
3 | 1 3

sparser
"Further mechanistic study revealed the interaction between USP37 and ERα: USP37 regulated ERα signaling through modulating protein stability instead of gene expression, in which it stabilized ERα protein via inhibiting the K48‐specific polyubiquitination process."

No evidence text available

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sparser
"Mechanistically, USP37 interacts with ERα and removes the K48‐linked ubiquitin through its catalytical activity, leading to the stabilization of ERα."

sparser
"These findings demonstrate that USP37 is an essential regulator of estrogen signaling and that the USP37ERα axis is a potential target for breast cancer treatment."

reach
"Further mechanistic study revealed the interaction between USP37 and ERalpha: USP37 regulated ERalpha signaling through modulating protein stability instead of gene expression, in which it stabilized ERalpha protein via inhibiting the K48-specific polyubiquitination process."
USP37 affects EPAS1
2 1 | 1 2 1
USP37 binds EPAS1.
2 | 1
2 | 1

No evidence text available

No evidence text available

sparser
"In Clear cell renal cell carcinoma (ccRCC), USP37 binds to and stabilizes HIF2α, promoting kidney cancer tumorigenesis [ xref ]."
USP37 deubiquitinates EPAS1.
1 | 1
USP37 leads to the deubiquitination of EPAS1. 2 / 2
1 | 1

"<span class="match term0">USP37</span> promotes deubiquitination of <span class="match term1">HIF2ALPHA</span> in kidney cancer"

reach
"USP37 promotes deubiquitination of HIF2alpha in kidney cancer."
USP37 activates EPAS1.
| 1 1
USP37 activates EPAS1. 2 / 2
| 1 1

reach
"As a result, USP37 promotes HIF2alpha protein stability in an enzymatically dependent manner, and depletion of USP37 leads to HIF2alpha down-regulation in ccRCC."

eidos
"Among these DUBs , USP37 induced the most significant increase in HIF2alpha levels ( Fig. 2D ) ."
| PMC
USP37 affects 14-3-3gamma
| 7
USP37 binds 14-3-3gamma.
| 3
USP37 binds 14-3-3gamma. 3 / 3
| 3

reach
"To determine the functional significance of the interaction between USP37 and 14-3-3gamma, we assessed whether the polyubiquitination of 14-3-3gamma was deubiquitinated by USP37."

reach
"Reciprocal co-IP confirmed that these two proteins interacted with each other, with USP37 strongly binding to 14-3-3gamma."

reach
"The results showed that of these six isoforms, only 14-3-3gamma bound to USP37, demonstrating that 14-3-3gamma is a unique binding partner of USP37."
USP37 increases the amount of 14-3-3gamma.
| 2
USP37 increases the amount of 14-3-3gamma. 2 / 2
| 2

reach
"USP37 dramatically increased the protein expression level of 14-3-3gamma in a dose dependent manner."

reach
"USP37 also increased the endogenous level of 14-3-3gamma in a dose dependent manner, as shown by the exogenous level."
USP37 decreases the amount of 14-3-3gamma.
| 1
USP37 decreases the amount of 14-3-3gamma. 1 / 1
| 1

reach
"As expected, the ubiquitination level of 14-3-3gamma was decreased by the catalytic activity of Myc-USP37."
USP37 activates 14-3-3gamma.
| 1
USP37 activates 14-3-3gamma. 1 / 1
| 1

reach
"These findings indicate that USP37 participates in the regulation of cell proliferation, and that USP37 induces stability of 14-3-3gamma."
GLI1 affects USP37
1 | 4 2
1 | 4 2

reach
"Further studies detected that USP37 also interacted with and stabilized Gli-1 protein, which is the main activator of Hedgehog target gene."

sparser
"MG132, CHX chase, immunofluorescence staining and co-immunoprecipitation assays were used to test the interaction between USP37 and Gli-1."

sparser
"Co-IP assay indicated that USP37 interacted with Gli-1(Fig. xref )."

reach
"MG132, CHX chase, immunofluorescence staining and co-immunoprecipitation assays were used to test the interaction between USP37 and Gli-1."

reach
"USP37 also interacted with and stabilized glioma-associated oncogene 1 (Gli-1) protein."

reach
"USP37 interacts with and stabilizes Gli-1 protein."

No evidence text available
FZR1 affects USP37
1 6 |
1 6 |

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"Here we show that USP37 binds the APC/C coactivator CDH1|Deubiquitinase USP37 is activated by CDK2 to antagonize APC(CDH1) and promote S phase entry"
ESR1 affects USP37
3 | 1 3
3 | 1 3

sparser
"Further mechanistic study revealed the interaction between USP37 and ERα: USP37 regulated ERα signaling through modulating protein stability instead of gene expression, in which it stabilized ERα protein via inhibiting the K48‐specific polyubiquitination process."

No evidence text available

No evidence text available

No evidence text available

sparser
"Mechanistically, USP37 interacts with ERα and removes the K48‐linked ubiquitin through its catalytical activity, leading to the stabilization of ERα."

sparser
"These findings demonstrate that USP37 is an essential regulator of estrogen signaling and that the USP37ERα axis is a potential target for breast cancer treatment."

reach
"Further mechanistic study revealed the interaction between USP37 and ERalpha: USP37 regulated ERalpha signaling through modulating protein stability instead of gene expression, in which it stabilized ERalpha protein via inhibiting the K48-specific polyubiquitination process."
CDC45 affects USP37
| 1 6
CDC45 binds USP37.
| 6
| 6

sparser
"The second highest pair is USP37-CDC45, which is consistent with our previous finding that USP37 interacts with CMG complexes xref ."

sparser
"Moreover, AlphaFold made strong reciprocal predictions that USP37 and CDC45 interact, highlighting CDC45 as a likely tethering point for USP37 at the replisome."

sparser
"Furthermore, consistent with the USP37-CDC45 interaction having an important function, the predicted binding surface of USP37, as well as the interacting residues, are highly conserved between human and Xenopus ( xref – xref )."

sparser
"To test the function of the predicted USP37-CDC45 interaction, we mutated eight highly conserved USP37 residues at the predicted interface to alanine, generating USP37 8A ( xref )."

sparser
"Finally, to monitor whether the USP37-CDC45 interaction is important, we monitored the sensitivity to genotoxic agents of USP37 KO cells complemented with an empty vector (E.V), USP37 WT , or USP37 8A ."

sparser
"Collectively, these data suggested that USP37’s interaction with CDC45 counteracts TRAIP-mediated CMG ubiquitylation, which mitigates the toxic effects of topoisomerase inhibitors."
CDC45 activates USP37.
| 1
CDC45 activates USP37. 1 / 1
| 1

reach
"Finally, guided by AlphaFold-Multimer, we discovered that binding to CDC45 mediates USP37's response to topological stress."
ZBTB16 affects USP37
| 4 1
ZBTB16 binds USP37.
| 2 1
| 2 1

reach
"We showed that USP37 interacted with PLZF and RARA through the PLZF moiety and sustained PLZF and RARA steady state levels."

reach
"In contrast, the deubiquitinating enzyme USP37 interacts with Plzf which increases Plzf protein stability [XREF_BIBR]."

sparser
"In contrast, the deubiquitinating enzyme USP37 interacts with Plzf which increases Plzf protein stability [ xref ]."
ZBTB16 increases the amount of USP37.
| 2
ZBTB16 increases the amount of USP37. 2 / 2
| 2

reach
"Interestingly, PLZF but not RARα levels are elevated in response to USP37 overexpression."
| PMC

reach
"In return, PLZF/RARα is likely to activate the expression of USP37."
USP37 affects vpx
| 6
USP37 inhibits vpx.
| 4
USP37 inhibits vpx. 4 / 4
| 4

reach
"Our study demonstrates that the deubiquitinase USP37 reverses Vpx- and TRIM21-mediated degradation of SAMHD1, thereby inhibiting SIV replication and LINE-1 activity by stabilizing SAMHD1."

reach
"In conclusion, we have discovered that USP37 extensively inhibited the Vpx-mediated degradation of SAMHD1 across various HIV-2/SIV subtypes."

reach
"USP37 reverses Vpx-mediated degradation of SAMHD1."

reach
"Further analysis showed that USP37 restored the inhibitory effect of Vpx on endogenous SAMHD1 (Fig. 8J)."
USP37 activates vpx.
| 2
USP37 activates vpx. 2 / 2
| 2

reach
"In this study, we demonstrated that deubiquitinase USP37 effectively inhibits the Vpx-mediated degradation and antiviral and anti-retrotransposition activities of SAMHD1 (Fig. 1, 2 and 8)."

reach
"Interestingly, USP37 could enhanced the stability of SAMHD1 in the absence of Vpx or SAMHD1 mutant resistance to Vpx-mediated degradation (Fig. 5B)."
| 1 5
| 1 4

reach
"In vivo experiment suggested that USP37 silencing suppressed the growth and lung metastasis of CRC in nude mice."

eidos
"USP37 is a modulator of the epithelial-mesenchymal transition and metastasis ."
| PMC

reach
"USP37 promotes angiogenesis and metastasis in colorectal cancer by facilitating β-catenin stability."

reach
"USP37 stabilizes Snail1 and thereby enhances the metastasis of gastric cancer [52]."

reach
"This was further validated in an orthotopic mouse model in which USP37 depletion reduces primary kidney tumorigenesis and decreases lung metastasis."
| PMC
| 1

reach
"Depletion of USP37 impairs ccRCC growth in 2D and 3D growth assays and in vivo kidney tumorigenesis and lung metastasis [XREF_BIBR]; therefore, inhibiting USP37 could be a viable therapeutic approach in VHL deficient or HIF-2alpha-dependent tumors."
| PMC
USP37 affects Flag
| 6
| 6

sparser
"Plasmids encoding Flag-USP37 and HA-Snail were co-expressed in 293T cells."

sparser
"Our studies showed that ectopically expressed Flag-USP37 was able to immunoprecipitate endogenous Snail protein in lung cancer H1299 cells ( xref )."

sparser
"We also expressed GST-Snail in E. coli BL21 cells, which was mixed with Flag-USP37 protein purified from 293T cells, and co-IP assays were carried out with GST beads."

sparser
"HA-Snail and Flag-USP37 were transiently expressed either separated or combined."

sparser
"Endogenous Cdt1 co-immunoprecipitated with overexpressed Flag-USP37 ( Figure 3 A)."

sparser
"Also, overexpression of an siRNA resistant version of Flag-USP37 rescued the drop in Cdt1 levels caused by USP37 depletion ( Supplemental Figure 4A )."
USP37 affects CDK2
2 | 1 3
2 | 1 3

sparser
"Since USP37 S 628 exists within a minimum CDK phosphorylation motif (S/T-P) ( Dephoure et al., 2008 ), CDK2 interacts with USP37 ( Figure 1 C), and CDK2 is active in G1/S, we investigated whether CDK[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In this study, we found that the CDK2-USP37 axis is a novel regulator for ERK1/2 through ERK1/2 stabilization."

reach
"Since USP37 S 628 exists within a minimum CDK phosphorylation motif (S/T-P) (Dephoure et al., 2008), CDK2 interacts with USP37, and CDK2 is active in G1/S, we investigated whether CDK2 phosphorylates [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Our study resulted in a model of the phosphorylation-deubiquitination cascade referred to as the CDK2USP37 axis that regulates the deubiquitination and stabilization of ERK1/2."

No evidence text available

No evidence text available
USP37 affects CDC45
| 6
| 6

sparser
"The second highest pair is USP37-CDC45, which is consistent with our previous finding that USP37 interacts with CMG complexes xref ."

sparser
"Moreover, AlphaFold made strong reciprocal predictions that USP37 and CDC45 interact, highlighting CDC45 as a likely tethering point for USP37 at the replisome."

sparser
"Furthermore, consistent with the USP37-CDC45 interaction having an important function, the predicted binding surface of USP37, as well as the interacting residues, are highly conserved between human and Xenopus ( xref – xref )."

sparser
"To test the function of the predicted USP37-CDC45 interaction, we mutated eight highly conserved USP37 residues at the predicted interface to alanine, generating USP37 8A ( xref )."

sparser
"Finally, to monitor whether the USP37-CDC45 interaction is important, we monitored the sensitivity to genotoxic agents of USP37 KO cells complemented with an empty vector (E.V), USP37 WT , or USP37 8A ."

sparser
"Collectively, these data suggested that USP37’s interaction with CDC45 counteracts TRAIP-mediated CMG ubiquitylation, which mitigates the toxic effects of topoisomerase inhibitors."
Flag affects USP37
| 6
| 6

sparser
"Plasmids encoding Flag-USP37 and HA-Snail were co-expressed in 293T cells."

sparser
"Our studies showed that ectopically expressed Flag-USP37 was able to immunoprecipitate endogenous Snail protein in lung cancer H1299 cells ( xref )."

sparser
"We also expressed GST-Snail in E. coli BL21 cells, which was mixed with Flag-USP37 protein purified from 293T cells, and co-IP assays were carried out with GST beads."

sparser
"HA-Snail and Flag-USP37 were transiently expressed either separated or combined."

sparser
"Endogenous Cdt1 co-immunoprecipitated with overexpressed Flag-USP37 ( Figure 3 A)."

sparser
"Also, overexpression of an siRNA resistant version of Flag-USP37 rescued the drop in Cdt1 levels caused by USP37 depletion ( Supplemental Figure 4A )."
CDKN1B affects USP37
| 1 5
| 1 5

sparser
"Ectopically expressed USP37 formed a complex with p27, promoted its stabilization, blocked cell proliferation and induced the expression of REST-target neuronal differentiation genes."

sparser
"Interaction between USP37 and p27 was also confirmed by co-immunoprecipitation experiments using anti-p27 antibodies or control IgG followed by Western blot analysis using anti-Ub antibodies."

reach
"Interaction between USP37 and p27 was also confirmed by co-immunoprecipitation experiments using anti-p27 antibodies or control IgG followed by Western blot analysis using anti-Ub antibodies."

sparser
"USP37 formed a complex with p27, promoted its deubiquitination and stabilization, and blocked cell proliferation [ xref ]."

sparser
"Ectopically expressed wild type USP37 formed a complex with p27, promoted its deubiquitination and stabilization and blocked cell proliferation."

sparser
"In medulloblastomas, REST expression could decrease cyclin-dependent kinase (CDK)NIB/p27 (a CDK inhibitor) by repressing ubiquitin specific peptidase 37 (USP37), which could form a complex with p27 to promote its deubiquitination and stabilization, and resulting in blocked cell proliferation [ xref ]."
USP37 affects stemness
| 5
USP37 activates stemness. 5 / 5
| 5

eidos
"USP37 knockdown suppressed stemness and chemo-resistance , which may assist clinical oncologists in designing and testing novel therapeutic strategies ."

eidos
"USP37 knockdown inhibits stemness , cell invasion and EMT via Hedgehog signaling pathway in breast cancer ."
| PMC

eidos
"USP37 knockdown inhibits stemness , cell invasion and EMT via Hedgehog signaling pathway in breast cancer ."

eidos
"The knockdown of USP37 could inhibit the stemness , cell invasion and EMT via downregulation of Hedgehog pathway ."

eidos
"Knockdown of USP37 expression hampered cell invasion , stemness , EMT and also resulted in the drug sensitivity to cisplatin ."
USP37 affects cell invasion
| 5
USP37 activates cell invasion. 5 / 5
| 5

eidos
"Knockdown of USP37 expression hampered cell invasion , stemness , EMT and also resulted in the drug sensitivity to cisplatin ."

eidos
"USP37 knockdown inhibits stemness , cell invasion and EMT via Hedgehog signaling pathway in breast cancer ."
| PMC

eidos
"USP37 knockdown inhibits stemness , cell invasion and EMT via Hedgehog signaling pathway in breast cancer ."

eidos
"The knockdown of USP37 could inhibit the stemness , cell invasion and EMT via downregulation of Hedgehog pathway ."

eidos
"These data confirm that USP37 mediates breast cancer stem-like properties , cell invasion and EMT via the Hh pathway ."
USP37 affects cell cycle
| 5
USP37 activates cell cycle.
| 3
| 3

reach
"We demonstrate that USP37 maintains active replisomes on S-phase chromatin and promotes normal cell cycle progression."

reach
"Thus, by stabilizing the expression of USP37, HBx promotes the accumulation of cyclin A to modulate cell cycle progression."

reach
"USP37 up-regulation promoted the proliferation, cell cycle progression, apoptosis inhibition, migration, invasion, epithelial mesenchymal transition (EMT) and stemness of CRC cells; moreover, USP37 facilitated the angiogenesis of human umbilical vein endothelial cells (HUVECs)."
USP37 inhibits cell cycle.
| 2
| 2

reach
"When tested on DLBCL cells, USP37 increased cell proliferation and inhibited cell cycle progression."

reach
"Established PLK1-dependent degradation substrates include EMI1 (Hansen et al. 2004; Moshe et al. 2004) and USP37 (Burrows et al. 2012), both of which antagonize the cell cycle E3 APC/C; WEE1 (Watanabe et al. 2004), which controls cyclin dependent kinase activity; and Claspin (Peschiaroli et al. 2006; Mailand et al. 2006; Mamely et al. 2006), which promotes DNA damage checkpoint signaling in S-phase."
USP37 affects UIMC1
| 5
USP37 inhibits UIMC1.
| 3
USP37 inhibits UIMC1. 3 / 3
| 3

reach
"We reasoned that USP26 and USP37 may antagonize the RAP80 dependent sequestration of BRCA1 by removing RNF8 and RNF168 mediated ubiquitylation and thereby promote HR."

reach
"Together, these results suggest that USP26 and USP37 promote the BRCA1 dependent loading of PALB2 and RAD51 by counteracting the repressive impact of RAP80 dependent BRCA1 sequestration and RAP80 dependent inhibition of end-resection during HR."

reach
"USP26 and USP37 have additionally been shown to impair accumulation of RAP80 and BRCA1 [154]."
USP37 activates UIMC1.
| 2
USP37 activates UIMC1. 2 / 2
| 2

reach
"Loss of USP26 or USP37 impairs HR by counteracting RAP80 dependent sequestration of BRCA1."

reach
"Together, this model may explain how USP26 and USP37 limit the repressive impact of RAP80 on HR."
USP37 affects CDH2
| 5
USP37 increases the amount of CDH2.
| 3
USP37 increases the amount of CDH2. 3 / 3
| 3

reach
"Silencing of USP37 expression induced MET including upregulated E-cadherin expression and downregulated N-cadherin expression, while PM reversed MET in silenced USP37 cells."

reach
"Immunofluorescence assay showed that overexpression of USP37 further inhibited E-cadherin expression and upregulated N-cadherin expression (Fig. 3j)."

reach
"As shown in Fig. 3c and d, knockdown of USP37 significantly decreased Snail1, N-cadherin and Vimentin expression, but increased E-cadherin expression levels."
USP37 decreases the amount of CDH2.
| 2
USP37 decreases the amount of CDH2. 2 / 2
| 2

reach
"The downregulation of USP37 decreased the N-cadherin levels slightly but no change in the E-cadherin levels was observed."

reach
"Immunofluorescence assay showed that overexpression of USP37 further inhibited E-cadherin expression and upregulated N-cadherin expression (Fig. 3j)."
USP37 affects 14-3-3γ
| 5
USP37 binds 14-3-3γ.
| 4
14-3-3γ binds USP37. 3 / 3
| 3

sparser
"The results showed that of these six isoforms, only 14-3-3γ bound to USP37, demonstrating that 14-3-3γ is a unique binding partner of USP37 (Figure xref , lane 7)."

sparser
"The identification of the interaction between USP37 and 14-3-3γ led us to investigate whether USP37 was associated with cell proliferation and migration via the regulation of 14-3-3γ."

sparser
"To determine the functional significance of the interaction between USP37 and 14-3-3γ, we assessed whether the polyubiquitination of 14-3-3γ was deubiquitinated by USP37."
14-3-3γ binds Hemagglutinins, USP37, and MYC. 1 / 1
| 1

sparser
"We used a cell counting kit-8 (CCK-8) to measure the cell viability and proliferation mediated by the interaction between HA-14-3-3γ and Myc-USP37."
USP37 ubiquitinates 14-3-3γ.
| 1
USP37 ubiquitinates 14-3-3γ. 1 / 1
| 1

sparser
"The findings on the regulation of 14-3-3γ ubiquitination by USP37 establish a new role for USP37 in cancer cell proliferation, indicating that ubiquitination, a PTM, has an important role in cancer."
SAMHD1 affects USP37
| 1 4
| 1 4

reach
"Overexpression of SAMHD1-Flag, but not DDB1, DCAF1, CUL4A, or CUL4B, pulled down USP37 in precipitates (Fig. 4C), indicating a specific interaction between USP37 and SAMHD1."

sparser
"USP37 interacts with SAMHD1 and removes polyubiquitination of SAMHD1."

sparser
"Overexpression of SAMHD1-Flag, but not DDB1, DCAF1, CUL4A, or CUL4B, pulled down USP37 in precipitates ( xref ), indicating a specific interaction between USP37 and SAMHD1."

sparser
"Taken together, these results indicate that USP37 specifically interacts with SAMHD1 and removes polyubiquitination of SAMHD1."

sparser
"Our results also showed that USP37 interacted with SAMHD1 but not with the E3 complex recruited by Vpx ( xref )."
SALL4 affects USP37
| 2 3
| 2 3

sparser
"Immunoprecipitates were eluted and then used for WB analysis to assess the interaction between USP37 and SALL4."

sparser
"The interaction between USP37 and SALL4 was confirmed by co-immunoprecipitation assay."

reach
"The interaction between USP37 and SALL4 was confirmed by co-immunoprecipitation assay."

sparser
"Besides, SALL4 level was enriched in anti-USP37, indicating that USP37 could bind to SALL4 protein (Fig.  xref H)."

reach
"The cell lysate was incubated with anti-IgG, anti-SALL4 and anti-USP37 for 2 h at room temperature, and then treated with protein A/G agarose (HY-K0230, MedChemExpress) for 1 h. Immunoprecipitates were eluted and then used for WB analysis to assess the interaction between USP37 and SALL4."
| 4 1
| 1 1

reach
"Early work showed that USP37 binds the APC/C CDH1 E3 complex to regulate ubiquitylation of cyclin A, a substrate of APC/C CDH1 [ 28 ]."

sparser
"Mutations in UIM2 and UIM3 abrogate binding to K48- and K63-linked Ub 4 chains, similar to published work [ 16 ], but also to K11-linked Ub 4 chains, which are produced by APC CDH1 , the E3 ligase a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
E3_Ub_ligase activates USP37.
| 2
| 2

reach
"E3 ligases that are active during exit from mitosis and therefore might target USP37 for degradation include beta-TRCP1, beta-TRCP2, APC CDC20, and APC CDH1 (Frescas and Pagano, 2008; Peters, 2002)."

reach
"The dichotomy in the action of two E3 ligases targeting USP37 further inspired us to investigate the possibility of direct or indirect interaction between HBx and USP37 which could reveal a bigger role of HBx in protection of USP37."
E3_Ub_ligase ubiquitinates USP37.
| 1
E3_Ub_ligase ubiquitinates USP37. 1 / 1
| 1

reach
"Inactive USP37 is ubiquitinated by E3 ubiquitin ligase APC and degraded in the proteasome (Huang et al., 2011)."
CHEK1 affects USP37
3 | 1 1
3 | 1 1

No evidence text available

No evidence text available

sparser
"Given that we have previously demonstrated USP37's interaction with CHK1 its ability to enhance CHK1 activity [ xref ], both of which are closely associated with replication fork movement, and that USP37 is also known to play a crucial role in the Homologous Recombination pathway [ xref ], we conducted a DNA fiber assay to evaluate replication fork movement at the single molecule level."

No evidence text available

reach
"We did observe deubiquitination of CHK1 in the same assay, consistent with earlier reports.Given that we have previously demonstrated USP37's interaction with CHK1 its ability to enhance CHK1 activity [23], both of which are closely associated with replication fork movement, and that USP37 is also known to play a crucial role in the Homologous Recombination pathway [55], we conducted a DNA fiber assay to evaluate replication fork movement at the single molecule level."
USP37 affects Snail1 protein
| 4
USP37 increases the amount of Snail1 protein.
| 2
Modified USP37 increases the amount of Snail1 protein. 2 / 2
| 2

reach
"Overexpression of USP37 in SGC7901-shRNA cells restores Snail1 protein levels, which shows that the USP37-Snail1 axis plays a vital role in PLAGL2 mediated Snail1 stabilization."

reach
"As shown in Figure XREF_FIG D and XREF_SUPPLEMENTARY A, depletion of USP37 in SGC7901 and HEK293T cells significantly reduced Snail1 protein expression, whereas enhanced USP37 expression in AGS and HEK293T cells dramatically upregulated the Snail1 protein levels."
USP37 deubiquitinates Snail1 protein.
| 1
USP37 deubiquitinates Snail1 protein. 1 / 1
| 1

reach
"PLAGL2 activated the transcription of deubiquitinase USP37, which then interacted with and deubiquitinated Snail1 protein directly."
USP37 decreases the amount of Snail1 protein.
| 1
USP37 decreases the amount of Snail1 protein. 1 / 1
| 1

reach
"As shown in Figure XREF_FIG D and XREF_SUPPLEMENTARY A, depletion of USP37 in SGC7901 and HEK293T cells significantly reduced Snail1 protein expression, whereas enhanced USP37 expression in AGS and HEK293T cells dramatically upregulated the Snail1 protein levels."
USP37 affects SMO
| 4
USP37 increases the amount of SMO.
| 2
USP37 increases the amount of SMO. 2 / 2
| 2

reach
"Interestingly, our data indicated that there was a time-dependent increase in the expression of Smo, Gli-1 and USP37 expression following 24 h, 48 h PM treatment (0.5 μM) (Fig. 6a)."

reach
"Upregulation of USP37 could enhance protein expression levels of Smo and Gli-1."
USP37 activates SMO.
| 2
USP37 activates SMO. 2 / 2
| 2

reach
"Knockdown of USP37 significantly decreased hedgehog (Hh) pathway components Smo and Gli-1."

reach
"We found that lower expression level of USP37 obviously impaired the expression of Hh targets (Smo and Gli-1) and cell proliferation marker Ki-67 in the tumor tissues as seen by immunohistochemical staining (Fig. 8e)."
USP37 affects REST
| 2 1
USP37 binds REST.
| 1 1
| 1 1

sparser
"However, the association of USP37 with REST needs further investigation."

reach
"Like p27, a reciprocal association between USP37 and REST is present, and REST KD increases USP37 mRNA levels."
| PMC
USP37 increases the amount of REST.
| 1
Modified USP37 increases the amount of REST. 1 / 1
| 1

reach
"Unexpectedly, Q-RT-PCR analyses showed that WT USP37 expression promoted a 2.7-8 fold increase in expression of the REST target genes Syn1, BDNF and SCG10 respectively, relative to vector transfected cells."
USP37 affects RAP80-BRCA1
| 1 3
USP37 inhibits RAP80-BRCA1. 4 / 4
| 1 3

reach
"USP37 and USP36 prevent excessive spreading of RAP80-BRCA1 from DSBs."
| PMC

eidos
"USP37 and USP36 prevent excessive spreading of RAP80-BRCA1 from DSBs ."
| PMC

reach
"USP26 or USP37 antagonize ubiquitin dependent spreading of RAP80-BRCA1."

reach
"We demonstrate that USP26 and USP37 prevent excessive spreading of RAP80-BRCA1 from DSBs."
USP37 affects PI3K
| 4
USP37 activates PI3K.
| 3
USP37 activates PI3K. 3 / 3
| 3

reach
"These data showed that USP37/SALL4 axis might activate PI3K/AKT pathway."

reach
"Moreover, USP37/SALL4 axis could increase the activity of PI3K/AKT pathway, and PI3K pathway inhibitor LY294002 abolished SALL4-mediated the promoting on keloid fibroblast functions."

reach
"USP37/SALL4 axis mediated the PI3K/AKT pathway."
USP37 increases the amount of PI3K.
| 1
USP37 increases the amount of phosphorylated PI3K. 1 / 1
| 1

reach
"Through WB analysis, we found that USP37 knockdown reduced the protein expression of p-PI3K/PI3K and p-AKT/AKT, whereas SALL4 reversed this effect (Fig. 6)."
USP37 affects Cyclin_A
| 2 2
USP37 deubiquitinates Cyclin_A.
| 2
USP37 deubiquitinates Cyclin_A. 2 / 2
| 2

reach
"USP37 has been shown to mediate deubiquitination of cyclin A thereby promoting S phase entry ( Huang et al., 2011 )."

reach
"For example, USP37 regulates the cell cycle by deubiquitinating and stabilizing cyclin A, which enables cells to enter into S phase (151, 152)."
USP37 binds Cyclin_A.
| 2

sparser
"Coimmunoprecipitation experiments indicated an interaction between endogenous USP37 and cyclin A in 293T cells ( Figure 3 C), making cyclin A a potential USP37 substrate."

sparser
"USP37 binds, deubiquitinates, and stabilizes cyclin A, thereby regulating the G1/S transition [ xref ]."
| 1 3
| 1 3

reach
"Conversely, USP37 and USP29 promote tumorigenesis by stabilizing c-Myc through deubiquitination [22, 23]."

eidos
"207 In addition to Fbw7 , other E3 ligases , such as beta-TrCP1 , CHIP and FBXO32 , can also ubiquitinate c-Myc , mediate its subsequent degradation and inhibit tumorigenesis.208-210 Moreover , the USP37 and USP36 can promote tumorigenesis by stabilizing c-Myc.211 ,212 By mass spectrometry , SUMO ligase protein inhibitor of activated STAT ( PIAS ) and Sentrin-specific protease 7 ( SENP7 ) were also found to control the SUMOylation of c-Myc at K326 and regulate its ubiquitination and degradation ( Fig. 2c ) .213 Ubiquitination regulates p53 p53 , one of the most important tumor suppressors , works in multiple cellular processes , such as cell cycle regulation , DNA repair and apoptosis ."

reach
"The role of USP37 in promoting tumorigenesis and how it regulates different substrates has been discussed in a recent review by our group, highlighting the importance of targeting USP37 for pharmacological intervention [22]."

reach
"This was further validated in an orthotopic mouse model in which USP37 depletion reduces primary kidney tumorigenesis and decreases lung metastasis."
| PMC
USP37 affects CDC27
2 | 1 1
2 | 1 1

reach
"Knockdown of CDH1 markedly reduced the interaction between USP37 and CDC27, suggesting that core APC/C components bind USP37 indirectly through CDH1."

sparser
"Knockdown of CDH1 markedly reduced the interaction between USP37 and CDC27 ( Figure 1 F), suggesting that core APC/C components bind USP37 indirectly through CDH1."

No evidence text available

No evidence text available
USP37 affects CDC20
1 | 2 1
1 | 2

No evidence text available

reach
"Huang et al. characterized the USP37 interaction with CDH1 and CDC20 by tandem mass spectrometry and found that USP37 interacts with CDH1 but not CDC20."
| PMC

reach
"HBx acts as a chaperone of USP37 and shuttles it out of the nucleus, where the ubiquitin E3 ligase CDC20 homolog 1 (CDH1) and b-TrCP associate with USP37 (Zhou et al., 2003; von Mikecz, 2006; Saxena and Kumar, 2014)."
CDH1 binds CDC20 and USP37. 1 / 1
| 1

sparser
"Huang et al. characterized the USP37 interaction with CDH1 and CDC20 by tandem mass spectrometry and found that USP37 interacts with CDH1 but not CDC20."
| PMC
USP37 affects CCNA2
1 2 1 |
USP37 deubiquitinates CCNA2.
1 1 |
USP37 deubiquitinates CCNA2. 2 / 2
1 1 |

"USP37 Binds, Deubiquitinates, and Stabilizes Cyclin A"

"Flow cytometry analysis of the HBx-expressing cells showed deregulation of cell cycle apparently due to the enhanced gene expression and stabilization of <span class="match term0">USP37</span> protein and deubiquitination of <span class="match term1">Cyclin A</span> by <span class="match term0">USP37</span>"
USP37 binds CCNA2.
2 |
2 |

No evidence text available

No evidence text available
TRAIP affects USP37
| 2 2
TRAIP binds USP37.
| 1 2
| 1 2

reach
"We propose that this regulation is critical to allow the completion of DNA replication and the suppression of genome instability.We uncovered a complex genetic interaction between USP37 and TRAIP."

sparser
"While TRAIP knockout and USP37 knockout cells were hypersensitive to CPT, as expected xref , xref , the USP37-TRAIP double knockout cells largely lost their hypersensitivity towards CPT ( xref and xref ), indicating that TRAIP loss compensates for the absence of USP37 and vice versa."

sparser
"We uncovered a complex genetic interaction between USP37 and TRAIP ."
TRAIP inhibits USP37.
| 1
TRAIP inhibits USP37. 1 / 1
| 1

reach
"Conversely, the CPT sensitivity in TRAIP knockouts was also largely suppressed by ablating USP37, demonstrating synthetic rescue in the other direction."
TFDP1 affects USP37
4 |
TFDP1 decreases the amount of USP37. 4 / 4
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
CDC27 affects USP37
2 | 1 1
2 | 1 1

reach
"Knockdown of CDH1 markedly reduced the interaction between USP37 and CDC27, suggesting that core APC/C components bind USP37 indirectly through CDH1."

sparser
"Knockdown of CDH1 markedly reduced the interaction between USP37 and CDC27 ( Figure 1 F), suggesting that core APC/C components bind USP37 indirectly through CDH1."

No evidence text available

No evidence text available
CDC20 affects USP37
1 | 2 1
1 | 2

No evidence text available

reach
"Huang et al. characterized the USP37 interaction with CDH1 and CDC20 by tandem mass spectrometry and found that USP37 interacts with CDH1 but not CDC20."
| PMC

reach
"HBx acts as a chaperone of USP37 and shuttles it out of the nucleus, where the ubiquitin E3 ligase CDC20 homolog 1 (CDH1) and b-TrCP associate with USP37 (Zhou et al., 2003; von Mikecz, 2006; Saxena and Kumar, 2014)."
CDH1 binds CDC20 and USP37. 1 / 1
| 1

sparser
"Huang et al. characterized the USP37 interaction with CDH1 and CDC20 by tandem mass spectrometry and found that USP37 interacts with CDH1 but not CDC20."
| PMC
14-3-3γ affects USP37
| 4
14-3-3γ binds USP37. 3 / 3
| 3

sparser
"The results showed that of these six isoforms, only 14-3-3γ bound to USP37, demonstrating that 14-3-3γ is a unique binding partner of USP37 (Figure xref , lane 7)."

sparser
"The identification of the interaction between USP37 and 14-3-3γ led us to investigate whether USP37 was associated with cell proliferation and migration via the regulation of 14-3-3γ."

sparser
"To determine the functional significance of the interaction between USP37 and 14-3-3γ, we assessed whether the polyubiquitination of 14-3-3γ was deubiquitinated by USP37."
14-3-3γ binds Hemagglutinins, USP37, and MYC. 1 / 1
| 1

sparser
"We used a cell counting kit-8 (CCK-8) to measure the cell viability and proliferation mediated by the interaction between HA-14-3-3γ and Myc-USP37."
3 |
Valproic acid decreases the amount of USP37. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
3 |
Hsa-miR-19b-3p decreases the amount of USP37. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
Gefitinib affects USP37
| 3
Gefitinib decreases the amount of USP37.
| 2
Gefitinib decreases the amount of USP37. 2 / 2
| 2

reach
"Gefitinib reduced expression levels of USP37, which mediated EGFR degradation similar to gefitinib."

reach
"Expression level of USP37 is negatively regulated by gefitinib in a time-dependent manner, and deletion of USP37 in gefitinib-treated cells synergistically enhances ubiquitination."
Gefitinib activates USP37.
| 1
| 1

reach
"Next, we investigated the upstream modulators of gefitinib-mediated USP37 down-regulation."
USP37 affects polyubiquitin chains
| 3
USP37 activates polyubiquitin chains.
| 2
USP37 activates polyubiquitin chains. 2 / 2
| 2

reach
"The DUB USP37 directly regulates S-phase entry through antagonising activity of the APC/C CDH1 in G 1 by removing polyubiquitin chains to stabilise CCNA [XREF_BIBR]."

reach
"Meanwhile, USP37 is phosphorylated and activated by CDK2, promoting the removal of polyubiquitin chains from ERK1/2 and resulting in the stabilization of ERK1/2.USP37 promotes cancer cell growth via ERK1/2 in vitro and in vivo."
USP37 inhibits polyubiquitin chains.
| 1
USP37 inhibits polyubiquitin chains. 1 / 1
| 1

reach
"Interestingly, RAP80 's access to polyubiquitin chains assembled by RNF8 and RNF168 is regulated by HR promoting factors such as RNF169, which competes with RAP80 for binding sites on polyubiquitin [XREF_BIBR], and the DUBs USP26 and USP37, which degrade polyubiquitin chains to reduce RAP80 accumulation [XREF_BIBR]."
USP37 affects migration
| 3
USP37 activates migration. 3 / 3
| 3

eidos
"On the contrary , upregulation of USP37 promoted EMT , migration and invasion ."

eidos
"In contrast , USP37 upregulation promotes EMT , migration , and invasion [ 39 ] ."
| PMC

eidos
"Accumulating evidence has demonstrated that USP37 promotes lung cancer cell proliferation , migration , and invasion16 but inhibits lung cancer cell apoptosis in a deubiquitination-dependent manner.19 Therefore , it is possible that IH-mediated miR-320b reduction in lung cancer cells resulted in the upregulation of USP37 , which further promoted cancer cell proliferation , invasion , and survival ."
USP37 affects TRAIP
| 1 2
| 1 2

reach
"We propose that this regulation is critical to allow the completion of DNA replication and the suppression of genome instability.We uncovered a complex genetic interaction between USP37 and TRAIP."

sparser
"While TRAIP knockout and USP37 knockout cells were hypersensitive to CPT, as expected xref , xref , the USP37-TRAIP double knockout cells largely lost their hypersensitivity towards CPT ( xref and xref ), indicating that TRAIP loss compensates for the absence of USP37 and vice versa."

sparser
"We uncovered a complex genetic interaction between USP37 and TRAIP ."
USP37 affects SMC3
2 | 1
2 |

No evidence text available

No evidence text available
| 1

sparser
"Identifying USP37-interacting partners with proximity-dependent biotin identification (BioID) found that USP37 interacts with the cohesin complex proteins SMC3, SMC1, SSCC1, and SA1/2, as well with the regulators of cohesion WAPL and NIPBL [ xref ]."
| PMC
USP37 affects RARA
| 3
USP37 activates RARA. 3 / 3
| 3

reach
"It was reported that USP37 induced the stability of the oncogenic fusion protein PLZF and RARA."

reach
"Subsequently, it was verified that USP37 expression modulated the oncogenic fusion protein PLZF/RARA stability and cell transformation potential in PLZF/RARA-associated acute promyelocytic leukemia [33]."

reach
"Furthermore, overexpression or depletion of USP37 caused an increase or decrease of PLZF and RARA protein half-life, correlating with down- or upregulation of PLZF and RARA poly-ubiquitination, respectively."
USP37 affects RAD51
| 3
USP37 activates RAD51. 3 / 3
| 3

reach
"Together, these results suggest that USP26 and USP37 promote the BRCA1 dependent loading of PALB2 and RAD51 by counteracting the repressive impact of RAP80 dependent BRCA1 sequestration and RAP80 dependent inhibition of end-resection during HR."

reach
"Interestingly, in contrast with initial findings assessing overexpression of these DUBs, knockdown of either USP26 or USP37 impairs accumulation of key HR factors including RAD51 as well as PALB2, CtIP, and RPA [154]."

reach
"Loss of USP26 and USP37 function markedly impairs the assembly of PALB2, RAD51 and efficient HR."
USP37 affects PLAGL2
| 2 1
| 2 1

reach
"The ChIP assay revealed that PLAGL2 was bound to the USP37 promoter region."

reach
"PLAGL2 specifically binds to the GRGGC(N)6-8RGGK consensus sequences in the USP37 promoter region and activates its transcription, subsequently stabilizing SNAI1 and promoting EMT in GC cells [64]."
| PMC

sparser
"The ChIP assay revealed that PLAGL2 was bound to the USP37 promoter region (Figure xref C)."
USP37 affects PALB2
| 3
USP37 activates PALB2. 3 / 3
| 3

reach
"Together, these results suggest that USP26 and USP37 promote the BRCA1 dependent loading of PALB2 and RAD51 by counteracting the repressive impact of RAP80 dependent BRCA1 sequestration and RAP80 dependent inhibition of end-resection during HR."

reach
"Loss of USP26 and USP37 function markedly impairs the assembly of PALB2, RAD51 and efficient HR."

reach
"Interestingly, in contrast with initial findings assessing overexpression of these DUBs, knockdown of either USP26 or USP37 impairs accumulation of key HR factors including RAD51 as well as PALB2, CtIP, and RPA [154]."
USP37 affects MCM
| 3
USP37 binds MCM.
| 2
| 2

reach
"Significantly, among all DUBs tested, only USP37 knockdown resulted in a nearly unimodal distribution of chromatin-bound MCM in late S/G2/M (Fig. 1F, Supplementary Fig. 2)."

reach
"USP37-depleted cells had the lowest level of chromatin-bound MCM in late S/G2/M cells, relative to all others tested (Fig. 1F)."
USP37 inhibits MCM.
| 1
USP37 inhibits MCM. 1 / 1
| 1

reach
"Our results suggest that the USP37 deubiquitinase restricts early MCM unloading and replisome disassembly through interactions with the replisome.The USP family of deubiquitinases is characterized by extensions and insertions into their conserved catalytic domains ."
USP37 affects Hedgehog
| 3
USP37 activates Hedgehog.
| 2
| 2

reach
"USP37 mediates hedgehog signaling pathway through stabilizing components such as Smo and GLI-1."

reach
"These findings are further supported by the ability of USP37 to participate in Hh signaling pathway in breast cancer."
USP37 increases the amount of Hedgehog.
| 1
USP37 increases the amount of Hedgehog. 1 / 1
| 1

reach
"We found that lower expression level of USP37 obviously impaired the expression of Hh targets (Smo and Gli-1) and cell proliferation marker Ki-67 in the tumor tissues as seen by immunohistochemical staining (Fig. 8e)."
| 2 1
| 1 1

reach
"Early work showed that USP37 binds the APC/C CDH1 E3 complex to regulate ubiquitylation of cyclin A, a substrate of APC/C CDH1 [ 28 ]."

sparser
"Mutations in UIM2 and UIM3 abrogate binding to K48- and K63-linked Ub 4 chains, similar to published work [ 16 ], but also to K11-linked Ub 4 chains, which are produced by APC CDH1 , the E3 ligase a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP37 inhibits E3_Ub_ligase.
| 1
| 1

reach
"A summary of these is described below and shown in Table 1.Deubiquitinases such as ubiquitin specific protease (USP) 28, USP36, and USP37 antagonize E3 ligase activity and stabilize c-Myc."
| 1 2
| 1 2

eidos
"Mechanistically , they found that USP37 counteracts APC / C-mediated ubiquitination of cyclin A , and that USP37 itself is phosphorylated by cyclin A-Cdk2 to increase its DUB activity and allow S-phase entry ."

reach
"Activation by phosphorylation also takes place during the cell cycle, where USP37 is modified by CDK-2 to directly stimulate DUB activity [74] ."

reach
"Subsequent phosphorylation of USP37 by either CDK2 and cyclin E or CDK2 and cyclin A triggers its full DUB activity."

reach
"USP37 and USP26 are recruited to DSBs to remove RNF168-induced ubiquitin conjugates from the BRCA1-A complex, whereas loss of USP37 impairs DSB repair."
| PMC

reach
"Moreover, over-expression of USP37 increased DSB end resection in control cells but not BLM depletion cells (Supplementary Figure S4K, L)."

reach
"In the context of DNA double-strand breaks (DSBs), USP37 and USP26 play pivotal roles in removing RNF168-induced ubiquitin conjugates from the BRCA1-A complex, without USP37 impairing DSB repair (22)."
| 3
USP37 activates Cell Survival.
| 2
| 2

reach
"Based on these observations, we propose that USP37 promotes genome integrity and cell survival by preventing premature disassembly of stressed replisome by TRAIP."

reach
"Previous studies reported that USP37 increased the Warburg effect and cell viability by repressing ubiquitin mediated degradation of c-Myc XREF_BIBR."
USP37 inhibits Cell Survival.
| 1

reach
"The depletion of USP37 reduced the cell viability compared with a control."
USP37 affects AKT
| 3
USP37 activates AKT. 3 / 3
| 3

reach
"USP37/SALL4 axis mediated the PI3K/AKT pathway."

reach
"These data showed that USP37/SALL4 axis might activate PI3K/AKT pathway."

reach
"Moreover, USP37/SALL4 axis could increase the activity of PI3K/AKT pathway, and PI3K pathway inhibitor LY294002 abolished SALL4-mediated the promoting on keloid fibroblast functions."
SMC3 affects USP37
2 | 1
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No evidence text available

No evidence text available
| 1

sparser
"Identifying USP37-interacting partners with proximity-dependent biotin identification (BioID) found that USP37 interacts with the cohesin complex proteins SMC3, SMC1, SSCC1, and SA1/2, as well with the regulators of cohesion WAPL and NIPBL [ xref ]."
| PMC
HNF4A affects USP37
3 |
HNF4A decreases the amount of USP37. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
EPAS1 affects USP37
2 | 1
2 | 1

No evidence text available

No evidence text available

sparser
"In Clear cell renal cell carcinoma (ccRCC), USP37 binds to and stabilizes HIF2α, promoting kidney cancer tumorigenesis [ xref ]."
CDK1/2i affects USP37
| 3
CDK1/2i decreases the amount of USP37.
| 2
CDK1/2i decreases the amount of USP37. 2 / 2
| 2

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"As shown in Fig. 6 C and Fig. S3 D, overexpression of USP37 resulted in an evident decrease in polyubiquitination of ERK1/2, while CDK1/2 inhibitors reversed this function.Intriguingly, we found that CDK1/2i treatment also markedly decreased USP37 protein levels but led to a significant increase in USP37 polyubiquitination (Fig. 6, A and B; and Fig. S3, A–C and E)."

reach
"Compared with USP37 wild type, the USP37 SA (S628A) mutant failed to deubiquitinate ERK1/2 and USP37 itself (Fig. 6, H and I; and Fig. S3 I), which may explain why CDK1/2i also decreased USP37 protein levels (Fig. 6, A and B; and Fig. S3, A–C)."
CDK1/2i deubiquitinates USP37.
| 1
CDK1/2i leads to the deubiquitination of USP37. 1 / 1
| 1

reach
"As shown in Fig. 6 C and Fig. S3 D, overexpression of USP37 resulted in an evident decrease in polyubiquitination of ERK1/2, while CDK1/2 inhibitors reversed this function.Intriguingly, we found that CDK1/2i treatment also markedly decreased USP37 protein levels but led to a significant increase in USP37 polyubiquitination (Fig. 6, A and B; and Fig. S3, A–C and E)."
14-3-3gamma affects USP37
| 3
USP37 binds 14-3-3gamma. 3 / 3
| 3

reach
"To determine the functional significance of the interaction between USP37 and 14-3-3gamma, we assessed whether the polyubiquitination of 14-3-3gamma was deubiquitinated by USP37."

reach
"Reciprocal co-IP confirmed that these two proteins interacted with each other, with USP37 strongly binding to 14-3-3gamma."

reach
"The results showed that of these six isoforms, only 14-3-3gamma bound to USP37, demonstrating that 14-3-3gamma is a unique binding partner of USP37."
2 |
Sodium arsenate increases the amount of USP37. 2 / 2
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No evidence text available

No evidence text available
2 |
Hsa-miR-30c-5p decreases the amount of USP37. 2 / 2
2 |

No evidence text available

No evidence text available
2 |
Hsa-miR-19a-3p decreases the amount of USP37. 2 / 2
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No evidence text available

No evidence text available
Bisphenol A affects USP37
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Bisphenol A decreases the amount of USP37. 2 / 2
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No evidence text available

No evidence text available
USP37 affects tumorigenicity
| 2
USP37 activates tumorigenicity. 2 / 2
| 2

eidos
"USP37 knockdown inhibits tumorigenicity and increases sensitivity to cisplatin in vivo ."

eidos
"Additionally , USP37 knockdown inhibited tumorigenicity and increased anticancer effect of cisplatin in vivo ."
USP37 affects tumor growth MCF-7 ADR cells
| 2
USP37 activates tumor growth MCF-7 ADR cells. 2 / 2
| 2

eidos
"By in vivo assay , USP37 downregulation also suppressed the tumor growth of MCF-7 / ADR cells ."

eidos
"Further experiment results showed that the downregulation of USP37 increased the sensitivity of MCF-7 / ADR cells to adriamycin in vitro and inhibited the tumor growth of MCF-7 / ADR cells in vivo ."

reach
"Downregulation of USP37 inhibits stemness, cell invasion and EMT via hedgehog signaling pathway in breast cancer."

eidos
"USP37 mediates hedgehog signaling pathway through stabilizing components such as Smo and GLI-1 ."

reach
"These findings are further supported by the ability of USP37 to participate in Hh signaling pathway in breast cancer."

reach
"3.1 Depletion of USP37 inhibits ERalpha signaling pathway activity."
| 2
| 2

reach
"Figure 6c shows that silencing of USP37 expression could reverse the positive effects of PM on the Hh pathway."

reach
"Meanwhile, knockdown of USP37 reversed the effect of PM on the EMT markers."

reach
"However, whether USP37 influences keloid formation through regulating SALL4 deubiquitination is unknown.Here, we hypothesized and demonstrated that USP37 deubiquitinated and stabilized SALL4 to activate PI3K/AKT pathway, thereby promoting proliferation, invasion, migration, extracellular matrix (ECM) accumulation and glycolysis in keloid fibroblasts."

reach
"USP37-activated SALL4 might enhance keloid fibroblast growth, invasion, migration, ECM accumulation and glycolysis via activating PI3K/AKT pathway."
USP37 affects gefitinib
| 2
| 2

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"miR-4487 Enhances Gefitinib-Mediated Ubiquitination and Autophagic Degradation of EGFR in Non-Small Cell Lung Cancer Cells by Targeting USP37."

reach
"These results suggest that the up-regulation of ubiquitination and autophagy by miR-4487/USP37 enhances gefitinib-mediated cytotoxicity and apoptotic cell death."
USP37 affects doxorubicin
| 2
| 2

reach
"USP37 downregulation elevates the Chemical Sensitivity of Human Breast Cancer Cells to Adriamycin."

reach
"The expression levels of USP37 in both MCF-7 and MCF-7 and ADR cells increased significantly with the exposure to adriamycin in a dose dependent manner."
USP37 affects TWNK
| 2
| 2

sparser
"We further identified the USP37 PH domain as a potential mediator of the USP37-replisome interaction."

sparser
"Therefore, we conclude that the PH domain is dispensable for USP37’s catalytic activity and localization to the nucleus but is vital for the USP37-replisome interaction."
USP37 affects STAG2
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No evidence text available

No evidence text available
USP37 affects SKP1
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2 |

No evidence text available

No evidence text available
USP37 affects SIVmac239 WT
| 2
USP37 inhibits SIVmac239 WT. 2 / 2
| 2

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"The results showed that overexpressing USP37 WT and the catalytic center USP37 (USP37-341-979) could effectively inhibit the production of SIVmac239 WT but has no significant effect on the production of SIVmac239 △Vpx (Fig. 8A and B)."

reach
"Conversely, USP37-knockdown increased the production of SIVmac239 WT, but it had no significant effect on the production of SIVmac239 △Vpx (Fig. 8C and D), indicating that USP37 suppresses the replication of SIVmac239 WT through inhibiting the Vpx-mediated degradation of SAMHD1."
USP37 affects RAD21
2 |
2 |

No evidence text available

No evidence text available
| 2
| 2

reach
"Our study suggests that targeting USP37 can induce synthetic lethality in osteosarcoma by disrupting its oncogenic network and inducing replication stress."

reach
"The KEGG and reactome pathway analysis showed that USP37 overexpression activates several distinct pathways in osteosarcoma cells, including Interferon signaling, Acute phase response signaling, Production of ROS, and HIF 1 alpha signaling (Additional file 1: Figure S4A, B)."
USP37 affects MITF
| 1 1
USP37 inhibits MITF. 2 / 2
| 1 1

reach
"An RNAi screen in HeLa cells of 296 genes that increase the MI revealed that depletion of USP37 markedly increases the MI, suggesting that it plays a role in mitotic progression [76]."
| PMC

eidos
"An RNAi screen in HeLa cells of 296 genes that increase the MI revealed that depletion of USP37 markedly increases the MI , suggesting that it plays a role in mitotic progression [ 76 ] ."
| PMC
USP37 affects LINE1
| 2
USP37 inhibits LINE1. 2 / 2
| 2

reach
"However, USP37 WT restored the ability of SAMHD1 to inhibit the LINE1 activity, but the C350A mutant only partially restored the SAMHD1 activity (Fig. 8I, lanes 5 and 6)."

reach
"To test whether USP37 affects the ability of SAMHD1 to inhibit LINE1, a well-established reporter system was used to evaluate the effect of SAMHD1 on LINE-1 retro-transposition."
USP37 affects LINE-1 elements
| 2
USP37 inhibits LINE-1 elements.
| 1
USP37 inhibits LINE-1 elements. 1 / 1
| 1

reach
"Additionally, USP37 enhances the suppression of the retrotransposition of LINE-1 elements by SAMHD1 via stabilizing SAMHD1."
USP37 activates LINE-1 elements.
| 1
USP37 activates LINE-1 elements. 1 / 1
| 1

reach
"Furthermore, USP37 enhances antiviral efficacy and suppresses retrotransposition of LINE-1 elements by stabilizing SAMHD1."
USP37 affects KITLG
| 1 1
USP37 inhibits KITLG.
| 1
USP37 inhibits KITLG. 1 / 1
| 1

reach
"However, HBx escorts USP37 from the nucleus, downregulates CDH1, and prevents its SCF mediated degradation via increased CDK2-mediated phosphorylation [24]."
| PMC
USP37 binds KITLG.
| 1
| 1

sparser
"Consistent with this hypothesis, USP37 interacts with components of the SCF in a βTrCP-dependent manner."
USP37 affects GST
| 2
| 2

sparser
"This revealed that purified GST-USP37 bound to FLAG-CDC73 ( xref B) and purified GST-CDC73 bound to Myc-USP37 ( xref C) in E. coli strain BL21."

sparser
"In addition, the binding between USP37 and ERK1/2 was further confirmed using bacterially expressed GST-USP37 proteins ( xref )."
USP37 affects ESR
| 2
USP37 activates ESR. 2 / 2
| 2

reach
"USP37 was shown to interact with ERα protein and to enhance the stability of ERα via removing its K48‐linked ubiquitin chain."

reach
"Collectively, these rescue experiments verified arguably that ERα overexpression restored the promoted activity of USP37 on breast cancer cell proliferation, suggesting that USP37 promoted ERα‐positive breast cancer cell proliferation via stabilizing ERα.4 DISCUSSION."
USP37 affects E3 APC/C
| 2
USP37 inhibits E3 APC/C. 2 / 2
| 2

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"Established PLK1-dependent degradation substrates include EMI1 and USP37, both of which antagonize the cell-cycle E3 APC/C; WEE1, which controls cyclin-dependent kinase activity; and Claspin, which promotes DNA-damage-checkpoint signaling in S phase."

reach
"Established PLK1-dependent degradation substrates include EMI1 (Hansen et al. 2004; Moshe et al. 2004) and USP37 (Burrows et al. 2012), both of which antagonize the cell cycle E3 APC/C; WEE1 (Watanabe et al. 2004), which controls cyclin dependent kinase activity; and Claspin (Peschiaroli et al. 2006; Mailand et al. 2006; Mamely et al. 2006), which promotes DNA damage checkpoint signaling in S-phase."
| 1 1
| 1 1

eidos
"These results indicated that USP37 downregulation attenuates breast cancer progression and enhances sensitivity to cisplatin in vivo ."

reach
"Our data demonstrated that depletion of USP37 by lentivirus‐based shRNA dramatically decreased the growth of breast tumor in vivo (Figure 6F)."
USP37 affects APC CDH1
| 2
USP37 inhibits APC CDH1.
| 1
USP37 inhibits APC CDH1. 1 / 1
| 1

reach
"In addition, USP37 preferentially antagonized the activity of APC CDH1 versus APC CDC20."
USP37 increases the amount of APC CDH1.
| 1
USP37 increases the amount of APC CDH1. 1 / 1
| 1

reach
"We show that USP37 is a DUB that associates with and antagonizes APC CDH1 following E2F mediated USP37 transcription in G1."
USP37 affects ANAPC7
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No evidence text available
USP37 affects ANAPC5
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No evidence text available

No evidence text available
UIM3 affects USP37
| 1 1
UIM3 activates USP37. 2 / 2
| 1 1

eidos
"Furthermore , UIM2 and UIM3 selectively bind to the proximal ubiquitin of K48-linked chains to enhance cleavage by USP37 , whereas UIM1 is not involved in substrate recognition [ 94 ] ."
| PMC

reach
"Therefore, the difference in the ability of the UIMs to bind ubiquitin plays a differential role on the kinetic parameters of USP37 depending on the type of ubiquitin chain, shedding light on the mechanism of substrate selection by USP37.When individual UIM mutants were tested, the authors found that UIM2 and UIM3 modulate the ability of USP37 to cleave all ubiquitin chain types, in contrast with that of UIM1."
| PMC
UIM2 affects USP37
| 1 1
UIM2 activates USP37. 2 / 2
| 1 1

reach
"Therefore, the difference in the ability of the UIMs to bind ubiquitin plays a differential role on the kinetic parameters of USP37 depending on the type of ubiquitin chain, shedding light on the mechanism of substrate selection by USP37.When individual UIM mutants were tested, the authors found that UIM2 and UIM3 modulate the ability of USP37 to cleave all ubiquitin chain types, in contrast with that of UIM1."
| PMC

eidos
"Furthermore , UIM2 and UIM3 selectively bind to the proximal ubiquitin of K48-linked chains to enhance cleavage by USP37 , whereas UIM1 is not involved in substrate recognition [ 94 ] ."
| PMC
TWNK affects USP37
| 2
| 2

sparser
"We further identified the USP37 PH domain as a potential mediator of the USP37-replisome interaction."

sparser
"Therefore, we conclude that the PH domain is dispensable for USP37’s catalytic activity and localization to the nucleus but is vital for the USP37-replisome interaction."
TFDP2 affects USP37
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TFDP2 decreases the amount of USP37. 2 / 2
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No evidence text available

No evidence text available
STAG2 affects USP37
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No evidence text available

No evidence text available
SKP1 affects USP37
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No evidence text available

No evidence text available
RB1 affects USP37
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RB1 decreases the amount of USP37. 2 / 2
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No evidence text available

No evidence text available
RAR affects USP37
| 2
RAR increases the amount of USP37. 2 / 2
| 2

reach
"In return, PLZF/RARα is likely to activate the expression of USP37."

reach
"Interestingly, PLZF but not RARα levels are elevated in response to USP37 overexpression."
| PMC
RAD21 affects USP37
2 |
2 |

No evidence text available

No evidence text available
PLK1 affects USP37
| 1 1
PLK1 leads to the phosphorylation of USP37. 2 / 2
| 1 1

reach
"The underlying mechanism involves PLK1 kinase-mediated phosphorylation of USP37 and its binding to βTrCP, promoting ubiquitination and degradation (Fig. 1)."
| PMC

sparser
"The underlying mechanism involves PLK1 kinase-mediated phosphorylation of USP37 and its binding to βTrCP, promoting ubiquitination and degradation (Fig. xref )."
| PMC
Neoplasms affects USP37
| 2
Neoplasms inhibits USP37.
| 1
| 1

reach
"Furthermore, miR-320b overexpression reduces IH-induced tumor growth by promoting USP37 downregulation."
| PMC
Neoplasms activates USP37.
| 1
| 1

reach
"Furthermore, miR-320b overexpression reduces IH-induced tumor growth by promoting USP37 downregulation."
| PMC
NRF1 affects USP37
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NRF1 decreases the amount of USP37. 2 / 2
2 |

No evidence text available

No evidence text available

reach
"In contrast, USP37 remained constant in nocodazole synchronized U2OS cells treated with MG132."

reach
"For this purpose, cells were transfected with HA-14-3-3gamma, and His Ubiquitin, with or without Myc-Usp37 and treated with MG132 before harvesting."
MCM affects USP37
| 2
| 2

reach
"Significantly, among all DUBs tested, only USP37 knockdown resulted in a nearly unimodal distribution of chromatin-bound MCM in late S/G2/M (Fig. 1F, Supplementary Fig. 2)."

reach
"USP37-depleted cells had the lowest level of chromatin-bound MCM in late S/G2/M cells, relative to all others tested (Fig. 1F)."
GST affects USP37
| 2
| 2

sparser
"This revealed that purified GST-USP37 bound to FLAG-CDC73 ( xref B) and purified GST-CDC73 bound to Myc-USP37 ( xref C) in E. coli strain BL21."

sparser
"In addition, the binding between USP37 and ERK1/2 was further confirmed using bacterially expressed GST-USP37 proteins ( xref )."
FOXG1 affects USP37
| 2
FOXG1 inhibits USP37.
| 1
FOXG1 inhibits USP37. 1 / 1
| 1

reach
"Qin et al. illustrated that miR-30b-5p repressed cell proliferation and arrested the cell cycle of a HCC cell line by targeting USP37 [54]."
FOXG1 activates USP37.
| 1
FOXG1 activates USP37. 1 / 1
| 1

reach
"Qin et al (42) reported that miR-30b-5p inhibited proliferation and slowed cell cycle progression of HCC by targeting DNMT3A and USP37."
FBXO5 affects USP37
| 1 1
FBXO5 activates USP37. 2 / 2
| 1 1

reach
"Accumulation of APC complex substrate E2F transcription factor targets EMI1, which completely inhibits APC and induces USP37 in the G1 phase (Fig. 1)."
| PMC

eidos
"Accumulation of APC complex substrate E2F transcription factor targets EMI1 , which completely inhibits APC and induces USP37 in the G1 phase ( Fig. 1 ) ."
| PMC
E2F4 affects USP37
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E2F4 decreases the amount of USP37. 2 / 2
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No evidence text available

No evidence text available
Cyclin_E affects USP37
| 2
Cyclin_E phosphorylates USP37 on S628. 1 / 1
| 1

reach
"In G1/S, Ser628 of USP37 is phosphorylated by either CDK2/cyclin E or CDK2/cyclin A, and this triggers USP37 full DUB activity."
Cyclin_E phosphorylates USP37. 1 / 1
| 1

reach
"Subsequent phosphorylation of USP37 by either CDK2 and cyclin E or CDK2 and cyclin A triggers its full DUB activity."
Cyclin_A affects USP37
| 2

sparser
"Coimmunoprecipitation experiments indicated an interaction between endogenous USP37 and cyclin A in 293T cells ( Figure 3 C), making cyclin A a potential USP37 substrate."

sparser
"USP37 binds, deubiquitinates, and stabilizes cyclin A, thereby regulating the G1/S transition [ xref ]."
CMG affects USP37
| 2
| 2

reach
"We therefore tested whether the USP37 PH domain is similarly important for USP37 binding to CMG."

reach
"Through a series of complementary approaches, we discovered that USP37 binds to the replisome, controls the level of polyubiquitin on MCM7 in cells, and prevents premature CMG unloading."
CCNA2 affects USP37
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No evidence text available

No evidence text available
ANAPC7 affects USP37
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No evidence text available

No evidence text available
ANAPC5 affects USP37
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No evidence text available

No evidence text available
Vinclozolin affects USP37
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Vinclozolin increases the amount of USP37. 1 / 1
1 |

No evidence text available
Various affects USP37
| 1
Various activates USP37. 1 / 1
| 1

eidos
"Our TCGA data analysis correlates with observation of various studies that elevated expression of USP37 in different cancers is requisite for cell proliferation and tumorigenesis ."
| PMC
VIRF-2 affects USP37
| 1
| 1

sparser
"Finally, specificity for Cdh1 may introduce possibilities for modulating the degradation of substrates via chain editing – for example, the DUB USP37 interacts with APC/CCdh1 to modulate K11 linkages on at least one substrate ( xref )."
Targeting KEN box degron affects USP37
| 1
Targeting KEN box degron inhibits USP37. 1 / 1
| 1

eidos
"B In the late G2 / M phase , USP37 acts as a substrate of the APC / C complex via phosphorylation by PLK1 and is further ubiquitinated by betaTrCP for the biphasic degradation , resulting in the downregulation of USP37 , necessary for the G2 / M phase transition Interestingly , the APC-CDH1 complex degrades USP37 in late mitosis by targeting its KEN box degron ."
| PMC
Resveratrol affects USP37
1 |
Resveratrol increases the amount of USP37. 1 / 1
1 |

No evidence text available
Protein deubiquitinase affects USP37
| 1
Protein deubiquitinase inhibits USP37. 1 / 1
| 1

reach
"Recently, REST repression of the gene encoding USP37, a protein deubiquitinase, has been proposed to play a key role in medulloblastoma generation."
Ppy affects USP37
| 1
| 1

sparser
"Further, the PH domain alone (PH-USP37) was sufficient to bind these core replisome proteins ( xref )."
Methyl methanesulfonate increases the amount of USP37. 1 / 1
1 |

No evidence text available
Mechanisms include affects USP37
| 1
Mechanisms include inhibits USP37. 1 / 1
| 1

eidos
"In addition , the G9a inhibitor UNC0638 impairs proliferation of human DAOY MB cells and MB growth in vivo through mechanisms that include downregulating USP37 [ 59,60 ] ."
| PMC
1 |
Hsa-mir-4775 decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-mir-4501 decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-mir-4280 decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-mir-4264 decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-mir-3164 decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-8060 decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-6828-5p decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-6809-3p decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-5580-5p decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-548av-3p decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-5003-3p decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-4753-3p decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-3591-5p decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-3118 decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-30b-5p decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-302d-5p decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-302b-5p decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-1910-5p decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-191-5p decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-185-3p decreases the amount of USP37. 1 / 1
1 |

No evidence text available
Hexabromocyclododecane decreases the amount of USP37. 1 / 1
1 |

No evidence text available
Haloperidol affects USP37
1 |
Haloperidol increases the amount of USP37. 1 / 1
1 |

No evidence text available
Ethyl methanesulfonate increases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Dorsomorphin increases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |
Dexamethasone decreases the amount of USP37. 1 / 1
1 |

No evidence text available
Coumarin affects USP37
1 |
Coumarin dephosphorylates USP37. 1 / 1
1 |

No evidence text available
Aristolochic acid A decreases the amount of USP37. 1 / 1
1 |

No evidence text available
1 |

No evidence text available
1 |
Aflatoxin B1 demethylates USP37. 1 / 1
1 |

No evidence text available
ZEB1 affects USP37
1 |
ZEB1 decreases the amount of USP37. 1 / 1
1 |

No evidence text available
WRNIP1 affects USP37
1 |
1 |

No evidence text available
WP1130 affects USP37
| 1
WP1130 inhibits USP37. 1 / 1
| 1

reach
"WP1130 could inhibit the DUB activity of USP9X, USP5, USP14, USP37 and USP24 [ 84 ]."
WDR82 affects USP37
1 |
1 |

No evidence text available
WDHD1 affects USP37
1 |
1 |

No evidence text available
WAPL affects Cohesin
| 1
| 1

sparser
"The authors further demonstrated that USP37 interacts with both cohesin and a negative regulator WAPL, which is required for releasing cohesin from chromatid arms in prophase."
VIRMA affects USP37
1 |
1 |

No evidence text available
Ub-binding site affects USP37
| 1
USP37 binds Ub-binding site. 1 / 1
| 1

reach
"This result is consistent with the likelihood that UbV.core directly competed for binding to the distal Ub binding site of USP37."
USP37 affects vIRF-2
| 1
| 1

sparser
"Finally, specificity for Cdh1 may introduce possibilities for modulating the degradation of substrates via chain editing – for example, the DUB USP37 interacts with APC/CCdh1 to modulate K11 linkages on at least one substrate ( xref )."
USP37 affects tumorigenicity MCF-7
| 1
USP37 activates tumorigenicity MCF-7. 1 / 1
| 1

eidos
"Furthermore , in vivo study showed that knockdown of USP37 expression also decreased tumorigenicity of MCF-7 / ADR cells in mice ."
USP37 affects tumorigenesis lung cancer
| 1
USP37 activates tumorigenesis lung cancer. 1 / 1
| 1

eidos
"Thus , we hypothesized that USP37 may contribute to the tumorigenesis of lung cancer by regulating CDT1 ."
USP37 affects tumor growth
| 1
USP37 activates tumor growth. 1 / 1
| 1

eidos
"Moreover , the DUB USP7 , USP13 , USP22 , USP28 , USP36 and USP37 stabilize c-Myc , thereby stimulating tumor growth [ 155 , 157 , 171-174 ] ."
| PMC
USP37 affects tumor MCF-7 ADR cells
| 1
USP37 activates tumor MCF-7 ADR cells. 1 / 1
| 1

eidos
"USP37 knockdown inhibits the tumor formation of MCF-7 / ADR cells in vivo The above results indicated that USP37 gene was overexpressed in adriamycin-resistant breast cancer cells and its downregulation could inhibit cell growth ."
USP37 affects stemness cell invasion EMT breast cancer
| 1
USP37 activates stemness cell invasion EMT breast cancer. 1 / 1
| 1

eidos
"Further studies indicated that USP37 knockdown could inhibit the stemness , cell invasion and EMT in breast cancer via downregulation of Hh pathway ."
| 1

reach
"However, these DUBs appear to lack specificity for KIFC1, as OTUD7B depletion showed no effect in either of our screens, and although USP37 depletion moderately increased multipolar spindle formation this was not associated with decreased KIFC1 levels (Appendix Table S1)."
USP37 affects spheroids
| 1
USP37 activates spheroids. 1 / 1
| 1

eidos
"In addition , USP37 knockdown significantly inhibited the formation of spheroids , as well as their size and volume , compared to the control cells in MCF-7 and MDA-MB-231 cells ( Fig. 4c and d ) ."
USP37 affects spheroid
| 1
USP37 inhibits spheroid. 1 / 1
| 1

eidos
"Furthermore , USP37 was also elevated at the protein level in cancer stem cell spheroids compared to adherent cells , and its knockdown ( KD ) decreased expression of stem cell markers like smoothened , Gli-1 , ALDH1 , and OCT4 and inhibited stable spheroid formation ."
| PMC
USP37 affects sensitivity cisplatin
| 1
USP37 inhibits sensitivity cisplatin. 1 / 1
| 1

eidos
"USP37 knockdown inhibits tumorigenicity and increases sensitivity to cisplatin in vivo ."

reach
"Additionally, USP37 enhances the suppression of the retrotransposition of LINE-1 elements by SAMHD1 via stabilizing SAMHD1."

reach
"These findings provide early evidence that USP37 modulates drug resistance."
| PMC
USP37 affects resistance breast cancer cells adriamycin
| 1
USP37 activates resistance breast cancer cells adriamycin. 1 / 1
| 1

eidos
"Conclusion : Knockdown of USP37 gene expression can reverse the resistance of breast cancer cells to adriamycin , and down-regulating USP37 might be a valuable strategy against ADR resistance in breast cancer therapy ."

reach
"We suggest this USP37-mediated mechanism complements other replication fork protection mechanisms to preserve replication capacity in S phase ."

reach
"These data suggest that USP26 and USP37 limit the magnitude of BRCA1-A complex assembly in DSB neighboring chromatin by counteracting RNF168 mediated H2A ubiquitylation."
| 1

reach
"Additionally, it is important to note that the introduction of a conserved cysteine residue in USP37 may have caused a change in protein folding and promoted its inactivation."
USP37 affects ppy
| 1
| 1

sparser
"Further, the PH domain alone (PH-USP37) was sufficient to bind these core replisome proteins ( xref )."
USP37 affects polyubiquitination protein
| 1
USP37 activates polyubiquitination protein. 1 / 1
| 1

eidos
"Subsequent sub-screening identified 23 DUBs that interact with SNAI1 , out of which USP29 , USP36 , and USP37 upregulate and reduce polyubiquitination of the SNAI1 protein ."
| PMC
USP37 affects oncogenic fusion PLZF-RARA
| 1
USP37 deubiquitinates oncogenic fusion PLZF-RARA. 1 / 1
| 1

reach
"Lastly, USP37 deubiquitinates and stabilizes the proto-oncogene c-Myc and the oncogenic fusion PLZF-RARA, suggesting that inhibition of USP37 DUB activity could have therapeutic potential XREF_BIBR, XREF_BIBR."
| 1

reach
"Functionally, overexpression of USP37 promotes cell proliferation and metabolism."
USP37 affects malignant
| 1
| 1

eidos
"As is the case with USP2 , it remains unknown if USP37 is upregulated or activated in cancer to promote malignant progression , or if targeting USP37 could be therapeutically beneficial in cancer ."
USP37 affects lung cancer cell
| 1
USP37 activates lung cancer cell. 1 / 1
| 1

eidos
"Accumulating evidence has demonstrated that USP37 promotes lung cancer cell proliferation , migration , and invasion16 but inhibits lung cancer cell apoptosis in a deubiquitination-dependent manner.19 Therefore , it is possible that IH-mediated miR-320b reduction in lung cancer cells resulted in the upregulation of USP37 , which further promoted cancer cell proliferation , invasion , and survival ."
USP37 affects invasion migration breast cancer cells
| 1
USP37 activates invasion migration breast cancer cells. 1 / 1
| 1

eidos
"Furthermore , upregulation of USP37 markedly promoted invasion and migration of breast cancer cells ( Fig. 3k and l ) ."
USP37 affects invading capacity MCF-7 MDA-MB-231 cells
| 1
USP37 activates invading capacity MCF-7 MDA-MB-231 cells. 1 / 1
| 1

eidos
"The transwell assay revealed that downregulation of USP37 obviously decreased the invading capacity of MCF-7 and MDA-MB-231 cells ."
USP37 affects intrinsic apoptosis including Bcl-2
| 1
USP37 inhibits intrinsic apoptosis including Bcl-2. 1 / 1
| 1

eidos
"More significantly , knockdown of USP37 will impair the adriamycin resistance of breast cancer cells and induce intrinsic apoptosis , including Bcl-2 / Bax / cleaved caspase 3 ."
USP37 affects hedgehog Hh pathway components Smo
| 1
USP37 activates hedgehog Hh pathway components Smo. 1 / 1
| 1

eidos
"Knockdown of USP37 significantly decreased hedgehog ( Hh ) pathway components Smo and Gli-1 ."
USP37 affects growth experimental MB
| 1
USP37 inhibits growth experimental MB. 1 / 1
| 1

eidos
"Expression of USP37 is downregulated in human MB , and USP37 reduces the growth of experimental MB ."
| PMC
USP37 affects entry
| 1
USP37 activates entry. 1 / 1
| 1

eidos
"Mechanistically , they found that USP37 counteracts APC / C-mediated ubiquitination of cyclin A , and that USP37 itself is phosphorylated by cyclin A-Cdk2 to increase its DUB activity and allow S-phase entry ."
USP37 affects drug sensitivity cisplatin
| 1
USP37 activates drug sensitivity cisplatin. 1 / 1
| 1

eidos
"Knockdown of USP37 expression hampered cell invasion , stemness , EMT and also resulted in the drug sensitivity to cisplatin ."
USP37 affects deubiquitination HIF2alpha kidney cancer
| 1
USP37 activates deubiquitination HIF2alpha kidney cancer. 1 / 1
| 1

eidos
"USP37 promotes deubiquitination of HIF2alpha in kidney cancer ."

reach
"Therefore, in addition to a role in sister chromatid resolution, both USP37 and WAPL contribute to normal chromosome segregation and bioriented kinetochore-microtubule attachment.Given these similar p[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP37 affects cellular
| 1
USP37 activates cellular. 1 / 1
| 1

eidos
"Knockdown of USP37 tremendously inhibited cellular proliferation as shown in Figure 3A ."
USP37 affects cellular breast cancer cells
| 1
USP37 activates cellular breast cancer cells. 1 / 1
| 1

eidos
"Through knockdown of USP37 expression in breast cancer MCF-7 cells and MCF-7 / ADR cells with a lentivirus vector system , we demonstrated that USP37 downregulation can evidently inhibit cellular proliferation of breast cancer cells ."
USP37 affects cell migration capacity
| 1
USP37 activates cell migration capacity. 1 / 1
| 1

eidos
"Knockdown of USP37 evidently inhibited cell migration capacity in both MCF and MDA-MB-231 cells ( Fig. 3f , g ) ."
USP37 affects cell growth
| 1
| 1

reach
"Thus, we examined whether knock-down of USP37 decreased cancer cell growth by downregulation of 14-3-3gamma."
USP37 affects breast cancer stem-like properties
| 1
USP37 activates breast cancer stem-like properties. 1 / 1
| 1

eidos
"These data confirm that USP37 mediates breast cancer stem-like properties , cell invasion and EMT via the Hh pathway ."
USP37 affects bioriented kinetochore-microtubule attachment
| 1
USP37 activates bioriented kinetochore-microtubule attachment. 1 / 1
| 1

eidos
"Additionally , identifying faulty kinetochore-microtubule attachments by error correction assay indicated that both USP37 and WAPL contribute to chromosomal segregation and bioriented kinetochore-microtubule attachment ."
| PMC
USP37 affects aphidicolin
| 1
| 1

reach
"We observed that overexpression of USP37 restored the viability of the knockout cells upon treatment with CPT (Fig. 1h), etoposide (Fig. 1i), or aphidicolin (Fig. 1j), while overexpression of the inactive mutant did not."
USP37 affects adriamycin resistance breast cancer cells
| 1
USP37 activates adriamycin resistance breast cancer cells. 1 / 1
| 1

eidos
"More significantly , knockdown of USP37 will impair the adriamycin resistance of breast cancer cells and induce intrinsic apoptosis , including Bcl-2 / Bax / cleaved caspase 3 ."
USP37 affects activation Hh pathway
| 1
USP37 activates activation Hh pathway. 1 / 1
| 1

eidos
"Therefore , it is a promising research to investigate whether USP37 promoted the activation of the Hh pathway ."
USP37 affects WRNIP1
1 |
1 |

No evidence text available
USP37 affects WDR82
1 |
1 |

No evidence text available
USP37 affects WDHD1
1 |
1 |

No evidence text available
USP37 affects VIRMA
1 |
1 |

No evidence text available
USP37 affects VIM
| 1
USP37 increases the amount of VIM. 1 / 1
| 1

reach
"As shown in Fig. 3c and d, knockdown of USP37 significantly decreased Snail1, N-cadherin and Vimentin expression, but increased E-cadherin expression levels."
USP37 affects Ub-binding site
| 1
USP37 binds Ub-binding site. 1 / 1
| 1

reach
"This result is consistent with the likelihood that UbV.core directly competed for binding to the distal Ub binding site of USP37."
USP37 affects USP25
| 1
USP25 binds USP37 and K48. 1 / 1
| 1

reach
"This suggests that the inter-UIM region is necessary for selective binding of USP25 and USP37 to K48 diUb."
USP37 affects TRIM4
1 |
1 |

No evidence text available
USP37 affects TRIM21
| 1
USP37 inhibits TRIM21. 1 / 1
| 1

reach
"Our study demonstrates that the deubiquitinase USP37 reverses Vpx- and TRIM21-mediated degradation of SAMHD1, thereby inhibiting SIV replication and LINE-1 activity by stabilizing SAMHD1."
USP37 affects TFF1
| 1
USP37 decreases the amount of TFF1. 1 / 1
| 1

reach
"We then used qPCR to examine Erα‐targeted genes and found that the consumption of USP37 markedly suppressed the transcription levels of PDZK1, GREB1, and PS2 in both cell lines (Figure 1D,E)."
USP37 affects TASOR
1 |
1 |

No evidence text available
USP37 affects TAF6
1 |
1 |

No evidence text available
USP37 affects TAF1
1 |
1 |

No evidence text available
USP37 affects Snail1 N-cadherin Vimentin
| 1
USP37 activates Snail1 N-cadherin Vimentin. 1 / 1
| 1

eidos
"As shown in Fig. 3c and d , knockdown of USP37 significantly decreased Snail1 , N-cadherin and Vimentin expression , but increased E-cadherin expression levels ."
USP37 affects STAG1
1 |
1 |

No evidence text available
USP37 affects SNU13
1 |
1 |

No evidence text available
USP37 affects SNRNP40
1 |
1 |

No evidence text available
USP37 affects SMC1A
1 |
1 |

No evidence text available
USP37 affects SMC1
| 1
| 1

sparser
"Identifying USP37-interacting partners with proximity-dependent biotin identification (BioID) found that USP37 interacts with the cohesin complex proteins SMC3, SMC1, SSCC1, and SA1/2, as well with the regulators of cohesion WAPL and NIPBL [ xref ]."
| PMC
USP37 affects SIRT1
1 |
1 |

No evidence text available
USP37 affects SF3B5
1 |
1 |

No evidence text available
USP37 affects SEPTIN7
| 1
USP37 leads to the ubiquitination of SEPTIN7. 1 / 1
| 1

reach
"In patients with lung cancer who also experienced OSA, IH induced a decrease in the expression of microRNA-320b, with downstream expression of ubiquitin-specific peptidase 37 (USP37) upregulated secondary to IH and USP37 promoting lung cancer progression by mediating the deubiquitination of Cdc10-dependent transcript 1 (CDT1) (98)."
USP37 affects SAP18
1 |
1 |

No evidence text available
USP37 affects S/G2/M
| 1
USP37 binds S/G2/M. 1 / 1
| 1

reach
"Significantly, among all DUBs tested, only USP37 knockdown resulted in a nearly unimodal distribution of chromatin-bound MCM in late S/G2/M (Fig. 1F, Supplementary Fig. 2)."
USP37 affects RNF8/168
| 1
USP37 inhibits RNF8/168. 1 / 1
| 1

reach
"Given that USP26 and USP37 are able to reverse RNF8/168 mediated ubiquitylation and promote HR, these enzymes would be ideal candidates to facilitate the repositioning of ubiquitylated substrates away from the DSB."
USP37 affects Pan et al., 2015
| 1
USP37 deubiquitinates Pan et al., 2015. 1 / 1
| 1

reach
"The previously reported deubiquiting enzyme USP37 can directly bind to and deubiquitinate c-Myc, thereby stabilizing c-Myc ( Pan et al., 2015 )."
USP37 affects PTCH1
| 1
| 1

sparser
"HBx acts as a chaperone of USP37 and shuttles it out of the nucleus, where the ubiquitin E3 ligase CDC20 homolog 1 (CDH1) and b-TrCP associate with USP37 (Zhou et al., xref ; von Mikecz, xref ; Saxena and Kumar, xref )."
USP37 affects PRPF19
1 |
1 |

No evidence text available
USP37 affects PRIM2
1 |
1 |

No evidence text available
USP37 affects POLR2H
1 |
1 |

No evidence text available
USP37 affects PNN
1 |
1 |

No evidence text available
USP37 affects PM EMT markers
| 1
USP37 activates PM EMT markers. 1 / 1
| 1

eidos
"Meanwhile , knockdown of USP37 reversed the effect of PM on the EMT markers ."
USP37 affects PHF2
1 |
1 |

No evidence text available
USP37 affects PAXBP1
1 |
1 |

No evidence text available
USP37 affects Neoplasms
| 1
| 1

reach
"USP37 KD in MCF7 cells also increased sensitivity to chemotherapeutic agents such as cisplatin by decreasing the BCL2/BAX ratio and attenuating tumor growth in vivo [39]."
| PMC
USP37 affects NOTCH1
1 |
1 |

No evidence text available
USP37 affects NIPBL
1 |
1 |

No evidence text available
USP37 affects MTREX
1 |
1 |

No evidence text available
USP37 affects MRPS31
1 |
1 |

No evidence text available
USP37 affects MIS18A
1 |
1 |

No evidence text available
USP37 affects MIR7-3HG
| 1
| 1

reach
"The results showed that USP37 knockdown significantly enhanced Sorafenib-triggered apoptosis in Huh7, SMMC-7721 and HepG2 cell lines (Fig. 4a and Figure S3 b,c)."
USP37 affects MCM6
| 1
USP37 leads to the ubiquitination of MCM6. 1 / 1
| 1

reach
"In the presence of the p97 inhibitor NMS-873 (p97-i), USP37 depletion led to polyubiquitylation of MCM7 and MCM6 on chromatin, which was most evident from loss of the unmodified forms of these proteins (Fig. 3b, lane 10 vs. 12)."
USP37 affects MCM4
1 |
1 |

No evidence text available
USP37 affects MCF-7 ADR cancer cells
| 1
USP37 inhibits MCF-7 ADR cancer cells. 1 / 1
| 1

eidos
"Our data demonstrated that USP37 downregulation enhanced the inhibitory effect of adriamycin on MCF-7 and MCF-7 / ADR cancer cells through the inhibition of Bcl-2 / Bax signaling pathway ."
USP37 affects KRT84
1 |
1 |

No evidence text available
USP37 affects K48
| 1
USP25 binds USP37 and K48. 1 / 1
| 1

reach
"This suggests that the inter-UIM region is necessary for selective binding of USP25 and USP37 to K48 diUb."
USP37 affects K11
| 1
USP37 binds C-Cdh1 and K11. 1 / 1
| 1

reach
"Finally, specificity for Cdh1 may introduce possibilities for modulating the degradation of substrates via chain editing - for example, the DUB USP37 interacts with APC and C-Cdh 1 to modulate K11 linkages on at least one substrate."
USP37 affects Infections
| 1
| 1

reach
"USP37 inhibits SIV infection and LINE1 retro-transposition activity by stabilizing SAMHD1."
USP37 affects INTS12
1 |
1 |

No evidence text available
USP37 affects ICRF-193
| 1
| 1

reach
"Consistent with this idea, depletion of TRAIP (Extended Data Fig. 5g) inhibited premature CMG unloading in USP37-depleted extracts containing ICRF-193 (Fig. 3c, lanes 4 vs. 6)."
USP37 affects Hypoxia
| 1
USP37 activates Hypoxia. 1 / 1
| 1

reach
"USP37 contributes to cancer progression by deubiquitinating and stabilizing hypoxia-inducible factor 2α (HIF2α), a key driver of tumor adaptation to hypoxic conditions, thereby promoting tumor growth, angiogenesis, and metastasis [23]."
USP37 affects Hh targets Smo Gli-1 cell marker Ki-67 tumor tissues
| 1
USP37 activates Hh targets Smo Gli-1 cell marker Ki-67 tumor tissues. 1 / 1
| 1

eidos
"We found that lower expression level of USP37 obviously impaired the expression of Hh targets ( Smo and Gli-1 ) and cell proliferation marker Ki-67 in the tumor tissues as seen by immunohistochemical staining ( Fig. 8e ) ."
| 1
14-3-3γ binds Hemagglutinins, USP37, and MYC. 1 / 1
| 1

sparser
"We used a cell counting kit-8 (CCK-8) to measure the cell viability and proliferation mediated by the interaction between HA-14-3-3γ and Myc-USP37."
USP37 affects HIF2alpha protein stability
| 1
USP37 activates HIF2alpha protein stability. 1 / 1
| 1

eidos
"As a result , USP37 promotes HIF2alpha protein stability in an enzymatically dependent manner , and depletion of USP37 leads to HIF2alpha down-regulation in ccRCC ."
USP37 affects HIF2alpha ccRCC
| 1
USP37 activates HIF2alpha ccRCC. 1 / 1
| 1

eidos
"As a result , USP37 promotes HIF2alpha protein stability in an enzymatically dependent manner , and depletion of USP37 leads to HIF2alpha down-regulation in ccRCC ."
USP37 affects HIF1
| 1
USP37 deubiquitinates HIF1. 1 / 1
| 1

reach
"Of note, USP37 specifically deubiquitinates HIF2α but not HIF1α (29)."
USP37 affects HDAC2
1 |
1 |

No evidence text available
USP37 affects HDAC1
1 |
1 |

No evidence text available
USP37 affects HA-14-3-3gamma
| 1
HA-14-3-3gamma binds USP37. 1 / 1
| 1

reach
"We used a cell counting kit-8 (CCK-8) to measure the cell viability and proliferation mediated by the interaction between HA-14-3-3gamma and Myc-USP37."
USP37 affects GTF3C3
1 |
1 |

No evidence text available
USP37 affects GREB1
| 1
USP37 decreases the amount of GREB1. 1 / 1
| 1

reach
"We then used qPCR to examine Erα‐targeted genes and found that the consumption of USP37 markedly suppressed the transcription levels of PDZK1, GREB1, and PS2 in both cell lines (Figure 1D,E)."
USP37 affects G1
| 1
USP37 binds APC/C Cdh1 and G1. 1 / 1
| 1

reach
"Usp37 binds APC/C Cdh1 in G1 and deubiquitinates the APC/C substrate cyclin A [XREF_BIBR]."
USP37 affects G1 phase
| 1
| 1

reach
"Flow cytometry analysis demonstrated that knocking down USP37 induced G1 phase arrest in MCF‐7 and T47D, indicating that USP37 may block the G1‐to‐S transition of breast cancer cells (Figure 6A,B)."
USP37 affects FlaG
| 1
| 1

sparser
"To test this possibility, we isolated FLAG-USP37 from HEK293T cells and incubated it with isolated Ub chains that are 4 ubiquitins in length (tetra-Ub, “Ub 4 ”)."
USP37 affects FBXW7
| 1
USP37 deubiquitinates FBXW7. 1 / 1
| 1

reach
"An early study showed that USP28 deubiquitinates c-Myc via interacting with Fbw7alpha whereas a recent study reveals that USP37 deubiquitinates c-Myc independently of Fbw7 and c-Myc phosphorylation."
USP37 affects EREG
| 1
USP37 increases the amount of EREG. 1 / 1
| 1

reach
"CCK8 assay demonstrated that the extent of suppressed growth of USP37 was remarkably reversed by upregulated Erα expression in MCF‐7 cells (Figure 7A)."
USP37 affects ECH1
1 |
1 |

No evidence text available
| 1

eidos
"These findings provide early evidence that USP37 modulates drug resistance ."
| PMC
USP37 affects DSB repair
| 1
USP37 activates DSB repair. 1 / 1
| 1

reach
"Loss of USP26 or USP37 impairs DSB repair."
USP37 affects DPY30
1 |
1 |

No evidence text available
USP37 affects DDR
| 1
USP37 activates DDR. 1 / 1
| 1

reach
"Knockdown of USP37 impairs the DDR through BLM and results in increased sensitivity to cisplatin or IR treatment in breast cancer cells."
USP37 affects Chemical Sensitivity Human Breast Cancer Cells
| 1
USP37 inhibits Chemical Sensitivity Human Breast Cancer Cells. 1 / 1
| 1

eidos
"USP37 downregulation elevates the Chemical Sensitivity of Human Breast Cancer Cells to Adriamycin Background : The evolution of adriamycin ( ADR ) resistance in the treatment of breast cancer often leads to a poor prognosis in patients ."
USP37 affects CTNNB1
| 1
USP37 increases the amount of CTNNB1. 1 / 1
| 1

reach
"Interestingly, we found that CTNNB1 (gene coding for β-catenin) level was positively correlated with USP37 level in CRC and USP37 silencing suppressed the expression of β-catenin in CRC cells and xenograft tumor tissues."
USP37 affects CHERP
1 |
1 |

No evidence text available
USP37 affects CDT1 protein
| 1
USP37 activates CDT1 protein. 1 / 1
| 1

eidos
"The results illustrated that USP37 knockdown significantly reduced CDT1 protein levels while lysosomal inhibitor ( MG132 ) preserved the CDT1 protein ( Figure 5M ) ."
USP37 affects CDT1 deubiquitination miR-320b IH-mediated lung cancer
| 1
USP37 inhibits CDT1 deubiquitination miR-320b IH-mediated lung cancer. 1 / 1
| 1

eidos
"USP37 promotes the expression of CDT1 by deubiquitination miR-320b impaired IH-mediated lung cancer progression by inhibiting CDT1 expression through targeting USP37 To understand whether the regulation of CDT1 expression by USP37 plays a role in lung cancer cell growth and invasion , the expressions of miR-320b and / or CDT1 in lung cancer cells ( A549 and H1650 ) were altered ."
USP37 affects CDC23
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No evidence text available
USP37 affects C/VIF2
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reach
"The inhibitory functions of vIRF-4 are dependent to 2 peptides regions, Vif-1 and Vif-2 of vIRF4 which can bind to USP-37 and inhibit its function as a p53 deubiquitinase."
USP37 affects C/VIF1
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reach
"The inhibitory functions of vIRF-4 are dependent to 2 peptides regions, Vif-1 and Vif-2 of vIRF4 which can bind to USP-37 and inhibit its function as a p53 deubiquitinase."
USP37 affects C-Cdh1
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USP37 binds C-Cdh1 and K11. 1 / 1
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reach
"Finally, specificity for Cdh1 may introduce possibilities for modulating the degradation of substrates via chain editing - for example, the DUB USP37 interacts with APC and C-Cdh 1 to modulate K11 linkages on at least one substrate."
USP37 affects BC
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USP37 inhibits BC. 1 / 1
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reach
"A study by Qin et al. reported that USP37 KD suppresses BC and BC stem cell migration and invasion by promoting the mesenchymal-epithelial transition (MET) by markedly reducing the SNAI1, N-cadherin, and vimentin expression and increasing the E-cadherin."
| PMC
USP37 affects AURKA
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No evidence text available
USP37 affects ATP5F1A
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No evidence text available
USP37 affects APC/C Cdh1
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USP37 binds APC/C Cdh1 and G1. 1 / 1
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reach
"Usp37 binds APC/C Cdh1 in G1 and deubiquitinates the APC/C substrate cyclin A [XREF_BIBR]."
USP37 affects ANAPC16
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No evidence text available
USP37 affects ANAPC13
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No evidence text available
USP37 affects ANAPC1
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No evidence text available
USP37 affects ALB
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No evidence text available
USP37 affects ADR cells
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USP37 activates ADR cells. 1 / 1
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eidos
"Knockdown of USP37 reduces the chemoresistance of MCF-7 and MCF-7 / ADR cells against adriamycin and activates the mechanism of apoptosis USP37 gene expression in both MCF-7 and MCF-7 / ADR cells was markedly upregulated by the exposure to adriamycin in a dose-dependent manner ( Figure 5A-D ) ."
USP37 affects ADR cells adriamycin
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USP37 activates ADR cells adriamycin. 1 / 1
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eidos
"In this study , we investigated whether USP37 knockdown could hamper the chemical resistance of MCF-7 and MCF-7 / ADR cells to adriamycin and elucidated the potential mechanism ."
USP37 affects ADNP
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No evidence text available
USP37 affects 3D
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USP37 inhibits 3D. 1 / 1
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reach
"Depletion of USP37 impairs ccRCC growth in 2D and 3D growth assays and in vivo kidney tumorigenesis and lung metastasis [XREF_BIBR]; therefore, inhibiting USP37 could be a viable therapeutic approach in VHL deficient or HIF-2alpha-dependent tumors."
| PMC
USP25 affects USP37
| 1
USP25 binds USP37 and K48. 1 / 1
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reach
"This suggests that the inter-UIM region is necessary for selective binding of USP25 and USP37 to K48 diUb."
TRIM4 affects USP37
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No evidence text available
TCF3 affects USP37
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TCF3 decreases the amount of USP37. 1 / 1
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No evidence text available
TASOR affects USP37
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No evidence text available
TAF6 affects USP37
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No evidence text available
TAF1 affects USP37
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No evidence text available
STAG1 affects USP37
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No evidence text available
SNU13 affects USP37
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No evidence text available
SNRNP40 affects USP37
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No evidence text available
SMC3 affects SMC1
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sparser
"Identifying USP37-interacting partners with proximity-dependent biotin identification (BioID) found that USP37 interacts with the cohesin complex proteins SMC3, SMC1, SSCC1, and SA1/2, as well with the regulators of cohesion WAPL and NIPBL [ xref ]."
| PMC
SMC1A affects USP37
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No evidence text available
SMC1 affects USP37
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sparser
"Identifying USP37-interacting partners with proximity-dependent biotin identification (BioID) found that USP37 interacts with the cohesin complex proteins SMC3, SMC1, SSCC1, and SA1/2, as well with the regulators of cohesion WAPL and NIPBL [ xref ]."
| PMC
SIRT1 affects USP37
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No evidence text available
SF3B5 affects USP37
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No evidence text available
SAP18 affects USP37
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No evidence text available
S/G2/M affects USP37
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USP37 binds S/G2/M. 1 / 1
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reach
"Significantly, among all DUBs tested, only USP37 knockdown resulted in a nearly unimodal distribution of chromatin-bound MCM in late S/G2/M (Fig. 1F, Supplementary Fig. 2)."
RNF168 affects USP37
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RNF168 ubiquitinates USP37. 1 / 1
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sparser
"USP26 and USP37 reverse RNF168-induced ubiquitylation at DSBs."
RARA affects USP37
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RARA activates USP37. 1 / 1
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reach
"The retinoic acid receptor alpha (RARA) and the promyelocytic leukemia zinc finger (PLZF) fusion protein is a target of USP37 and the stabilization of PLZF and oncogenic transformation of acute promyelocytic leukemia cells has been attributed to USP37."
Particulate Matter increases the amount of USP37. 1 / 1
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No evidence text available
PTCH1 affects USP37
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sparser
"HBx acts as a chaperone of USP37 and shuttles it out of the nucleus, where the ubiquitin E3 ligase CDC20 homolog 1 (CDH1) and b-TrCP associate with USP37 (Zhou et al., xref ; von Mikecz, xref ; Saxena and Kumar, xref )."
PRPF19 affects USP37
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No evidence text available
PROC affects USP37
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PROC ubiquitinates USP37. 1 / 1
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reach
"Inactive USP37 is ubiquitinated by E3 ubiquitin ligase APC and degraded in the proteasome (Huang et al., 2011)."
PRIM2 affects USP37
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No evidence text available
PPARG affects USP37
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PPARG decreases the amount of USP37. 1 / 1
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No evidence text available
PPARA affects USP37
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PPARA decreases the amount of USP37. 1 / 1
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No evidence text available
POLR2H affects USP37
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No evidence text available
PNN affects USP37
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No evidence text available
PHF2 affects USP37
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No evidence text available
PAXBP1 affects USP37
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No evidence text available
Overexpression miR-320b affects USP37
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Overexpression miR-320b inhibits USP37. 1 / 1
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eidos
"Overexpression of miR-320b downregulates USP37 in an in vivo xenograft model , resulting in repression of the CDT1 inhibition of LC progression ."
| PMC
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reach
"(Additional file 1: Figure S2C, D) as we have recently shown that USP37 regulates tolerance of replication stress by enhancing CHK1 activity so in order to gain insight into genes that correlated with knock down or overexpression of USP37, we proceeded with exome sequencing analysis in osteosarcoma cells overexpressing USP37 or in cells in which USP37 was depleted."
NOTCH1 affects USP37
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No evidence text available
NIPBL affects USP37
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No evidence text available
NANOG affects USP10, USP16, USP3, USP37, USP44, and USP7
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sparser
"USP25, USP44, USP49, and USP7 bind to the Sox2 promoter, while USP10, USP16, USP3, USP37, USP44, and USP7 bind to the Nanog promoter."
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14-3-3γ binds Hemagglutinins, USP37, and MYC. 1 / 1
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sparser
"We used a cell counting kit-8 (CCK-8) to measure the cell viability and proliferation mediated by the interaction between HA-14-3-3γ and Myc-USP37."
MTREX affects USP37
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No evidence text available
MRPS31 affects USP37
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No evidence text available
MIS18A affects USP37
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No evidence text available
MIR606 affects USP37
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MIR606 decreases the amount of USP37. 1 / 1
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No evidence text available
MCM7 affects USP37
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sparser
"To explore this hypothesis, we first established that FLAG-tagged USP37 can interact with V5 epitope-tagged MCM7 when expressed in HEK293T cells ( xref )."
MCM4 affects USP37
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No evidence text available
KRT84 affects USP37
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No evidence text available
KITLG affects USP37
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sparser
"Consistent with this hypothesis, USP37 interacts with components of the SCF in a βTrCP-dependent manner."
K48 affects USP37
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USP25 binds USP37 and K48. 1 / 1
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reach
"This suggests that the inter-UIM region is necessary for selective binding of USP25 and USP37 to K48 diUb."
K11 affects USP37
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USP37 binds C-Cdh1 and K11. 1 / 1
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reach
"Finally, specificity for Cdh1 may introduce possibilities for modulating the degradation of substrates via chain editing - for example, the DUB USP37 interacts with APC and C-Cdh 1 to modulate K11 linkages on at least one substrate."
Ile-His affects USP37
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Ile-His activates USP37. 1 / 1
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reach
"XREF_BIBR Therefore, it is possible that IH mediated miR-320b reduction in lung cancer cells resulted in the upregulation of USP37, which further promoted cancer cell proliferation, invasion, and survival."
INTS12 affects USP37
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No evidence text available
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14-3-3γ binds Hemagglutinins, USP37, and MYC. 1 / 1
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sparser
"We used a cell counting kit-8 (CCK-8) to measure the cell viability and proliferation mediated by the interaction between HA-14-3-3γ and Myc-USP37."
HDAC2 affects USP37
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No evidence text available
HDAC1 affects USP37
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No evidence text available
HA-14-3-3gamma affects USP37
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HA-14-3-3gamma binds USP37. 1 / 1
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reach
"We used a cell counting kit-8 (CCK-8) to measure the cell viability and proliferation mediated by the interaction between HA-14-3-3gamma and Myc-USP37."
GTF3C3 affects USP37
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No evidence text available
G9a pharmacological affects USP37
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G9a pharmacological inhibits USP37. 1 / 1
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eidos
"Furthermore , inhibiting G9a activity with pharmacological inhibitors reactivate USP37 expression , stabilizing p27 and its target genes ( Fig. 4 ) ."
| PMC
G1 affects USP37
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USP37 binds APC/C Cdh1 and G1. 1 / 1
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reach
"Usp37 binds APC/C Cdh1 in G1 and deubiquitinates the APC/C substrate cyclin A [XREF_BIBR]."
FlaG affects USP37
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sparser
"To test this possibility, we isolated FLAG-USP37 from HEK293T cells and incubated it with isolated Ub chains that are 4 ubiquitins in length (tetra-Ub, “Ub 4 ”)."
FBXW11 affects KITLG, and USP37
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sparser
"Consistent with this hypothesis, USP37 interacts with components of the SCF in a βTrCP-dependent manner."
EHMT2 affects USP37
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EHMT2 inhibits USP37. 1 / 1
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eidos
"G9a reduces USP37 expression by promoting H3K9 mono - , di - , and trimethylation at its promoter ."
| PMC
EGR2 affects USP37
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EGR2 decreases the amount of USP37. 1 / 1
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No evidence text available
ECH1 affects USP37
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No evidence text available

reach
"The failure of the HDAC inhibitor, MS-275, to upregulate USP37 expression suggests that either HDAC1/2 activities are not necessary or are alone insufficient to de-repress USP37 expression (XREF_FIG)."
DPY30 affects USP37
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No evidence text available
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D-aspartic acid increases the amount of USP37. 1 / 1
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reach
"Notably, Das et al. reported that REST induces medulloblastoma oncogenesis by repressing USP37 transcription, thereby leading to low levels of the p27 tumor suppressor, which controls proliferation and cell cycle exit by inhibiting CDK1 in cerebellar progenitor cells."
| PMC
Cyclin affects USP37
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Cyclin activates USP37. 1 / 1
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reach
"There is positive reinforcement of this signaling mechanism because phosphorylation of Ser 628 by CDK2 and cyclin E and CDK2 and cyclin A complexes produces maximal USP37 activity, and USP37 activates[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
Cohesin affects WAPL
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sparser
"The authors further demonstrated that USP37 interacts with both cohesin and a negative regulator WAPL, which is required for releasing cohesin from chromatid arms in prophase."
Cohesin affects SMC3
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sparser
"Identifying USP37-interacting partners with proximity-dependent biotin identification (BioID) found that USP37 interacts with the cohesin complex proteins SMC3, SMC1, SSCC1, and SA1/2, as well with the regulators of cohesion WAPL and NIPBL [ xref ]."
| PMC
| 1

reach
"Therefore, USP37 drives oncogenesis by altering the stability of various oncoproteins and activating downstream signaling pathways."
| PMC
CPT affects USP37
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CPT inhibits USP37. 1 / 1
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reach
"Conversely, the CPT sensitivity in TRAIP knockouts was also largely suppressed by ablating USP37, demonstrating synthetic rescue in the other direction."
CHERP affects USP37
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No evidence text available
CDKN affects USP37
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CDKN decreases the amount of USP37. 1 / 1
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reach
"Repressing USP37 expression results in the accelerated turnover of p27 (kip1), a cyclin-dependent kinase inhibitor that halts cell proliferation."
CDK2 inhibitor affects USP37
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CDK2 inhibitor increases the amount of USP37. 1 / 1
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reach
"In addition, CDK2 inhibitor II (Nakamura et al., 2007) treatment of HeLa cells arrested in G1/S decreased the amount of active USP37, but not total DUB activity."
CDH1 affects vIRF-2
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sparser
"Finally, specificity for Cdh1 may introduce possibilities for modulating the degradation of substrates via chain editing – for example, the DUB USP37 interacts with APC/CCdh1 to modulate K11 linkages on at least one substrate ( xref )."
CDH1 affects CDC20
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CDH1 binds CDC20 and USP37. 1 / 1
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sparser
"Huang et al. characterized the USP37 interaction with CDH1 and CDC20 by tandem mass spectrometry and found that USP37 interacts with CDH1 but not CDC20."
| PMC
CDH1 affects APC_C
| 1
| 1

sparser
"Finally, specificity for Cdh1 may introduce possibilities for modulating the degradation of substrates via chain editing – for example, the DUB USP37 interacts with APC/CCdh1 to modulate K11 linkages on at least one substrate ( xref )."
CDC23 affects USP37
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No evidence text available
CDC20 affects CDH1, and USP37
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CDH1 binds CDC20 and USP37. 1 / 1
| 1

sparser
"Huang et al. characterized the USP37 interaction with CDH1 and CDC20 by tandem mass spectrometry and found that USP37 interacts with CDH1 but not CDC20."
| PMC
C/VIF2 affects USP37
| 1
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reach
"The inhibitory functions of vIRF-4 are dependent to 2 peptides regions, Vif-1 and Vif-2 of vIRF4 which can bind to USP-37 and inhibit its function as a p53 deubiquitinase."
C/VIF1 affects USP37
| 1
| 1

reach
"The inhibitory functions of vIRF-4 are dependent to 2 peptides regions, Vif-1 and Vif-2 of vIRF4 which can bind to USP-37 and inhibit its function as a p53 deubiquitinase."
C-Cdh1 affects USP37
| 1
USP37 binds C-Cdh1 and K11. 1 / 1
| 1

reach
"Finally, specificity for Cdh1 may introduce possibilities for modulating the degradation of substrates via chain editing - for example, the DUB USP37 interacts with APC and C-Cdh 1 to modulate K11 linkages on at least one substrate."
BRCA1 affects USP37
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No evidence text available
AURKA affects USP37
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No evidence text available
ATP5F1A affects USP37
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No evidence text available
APC_C affects vIRF-2
| 1
| 1

sparser
"Finally, specificity for Cdh1 may introduce possibilities for modulating the degradation of substrates via chain editing – for example, the DUB USP37 interacts with APC/CCdh1 to modulate K11 linkages on at least one substrate ( xref )."
APC/C Cdh1 affects USP37
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USP37 binds APC/C Cdh1 and G1. 1 / 1
| 1

reach
"Usp37 binds APC/C Cdh1 in G1 and deubiquitinates the APC/C substrate cyclin A [XREF_BIBR]."
APC-CDH1 complex affects USP37
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APC-CDH1 complex inhibits USP37. 1 / 1
| 1

eidos
"B In the late G2 / M phase , USP37 acts as a substrate of the APC / C complex via phosphorylation by PLK1 and is further ubiquitinated by betaTrCP for the biphasic degradation , resulting in the downregulation of USP37 , necessary for the G2 / M phase transition Interestingly , the APC-CDH1 complex degrades USP37 in late mitosis by targeting its KEN box degron ."
| PMC
APC CDH1 affects USP37
| 1
APC CDH1 increases the amount of USP37. 1 / 1
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reach
"We show that USP37 is a DUB that associates with and antagonizes APC CDH1 following E2F mediated USP37 transcription in G1."
ANAPC16 affects USP37
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No evidence text available
ANAPC13 affects USP37
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1 |

No evidence text available
ANAPC1 affects USP37
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No evidence text available
ALPI affects USP37
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ALPI inhibits USP37. 1 / 1
| 1

reach
"These slow migrating species were phosphorylated USP37 because the bands disappeared from lysates treated with calf intestinal alkaline phosphatase."
ALB affects USP37
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No evidence text available
AHR affects USP37
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AHR decreases the amount of USP37. 1 / 1
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No evidence text available
ADNP affects USP37
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No evidence text available
4-hydroxynon-2-enal decreases the amount of USP37. 1 / 1
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No evidence text available
2-palmitoylglycerol increases the amount of USP37. 1 / 1
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No evidence text available

No evidence text available
17alpha-ethynylestradiol increases the amount of USP37. 1 / 1
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No evidence text available
14-3-3γ affects MYC
| 1
14-3-3γ binds Hemagglutinins, USP37, and MYC. 1 / 1
| 1

sparser
"We used a cell counting kit-8 (CCK-8) to measure the cell viability and proliferation mediated by the interaction between HA-14-3-3γ and Myc-USP37."
(R)-pantothenic acid decreases the amount of USP37. 1 / 1
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No evidence text available
1 |

No evidence text available