USP3 affects DDX58

2 1 | 17 6

USP3 binds DDX58.

2 | 5 5

USP3 binds DDX58. 7 / 7

2 | 5

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biogrid

No evidence text available

24366338

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➶

reach

"Because it was recently shown that USP3 interacts with RIG-I to deubiquitinate K63 linked poly-ubiquitin chains on RIG-I and inhibit type I IFN pathways [XREF_BIBR], we focused on SRSF1 for further experiments."

25658361

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➶

reach

"We found that USP3 and a splicing-factor oncoprotein, serine/arginine rich splicing factor 1 (SRSF1), interact with RIG-I."

25658361

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➶

biogrid

No evidence text available

24366338

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➶

reach

"Coimmunoprecipitation and western blot analyses revealed that USP3 and SRSF1 specifically interact with RIG-I in vitro (XREF_FIG and XREF_SUPPLEMENTARY)."

25658361

---

➶

reach

"USP3 binds RIG-I and removes the K63-polyubiquitin chains on the N-terminal CARDs [XREF_BIBR]."

28295214

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➶

reach

"Upon virus infection, USP3 interacted with RIG-I, likely acting as a negative feedback regulator."

25753787

USP3 binds IFIH1 and DDX58. 3 / 3

| 3

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➶

sparser

"The interaction of USP3 with RIG-I and MDA5 is strictly based upon ligand stimulation."

33138315

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➶

sparser

"Also co-IP demonstrated the interaction between USP3 and stimulated RIG-I or MDA5, but not the unstimulated ones, supporting that ligand stimulation is required for USP3 to interact with RIG-I or MDA5 [ xref ]."

31208841

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➶

sparser

"Also co-IP demonstrated the interaction between USP3 and stimulated RIG-I or MDA5, but not the unstimulated ones, supporting that ligand stimulation is required for USP3 to interact with RIG-I or MDA5 [25]."

31208841

UBC binds USP3 and DDX58. 1 / 1

| 1

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➶

sparser

"Because it was recently shown that USP3 interacts with RIG-I to deubiquitinate K63-linked poly-ubiquitin chains on RIG-I and inhibit type I IFN pathways [ xref ], we focused on SRSF1 for further experiments."

25658361

SRSF1 binds USP3 and DDX58. 1 / 1

| 1

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➶

sparser

"To examine which proteins directly interact with RIG-I, we transfected 293T cells with HA-tagged candidate genes together with Flag-tagged RIG-I. Coimmunoprecipitation and western blot analyses revealed that USP3 and SRSF1 specifically interact with RIG-I in vitro ( xref and xref )."

25658361

USP3 inhibits DDX58.

| 7 1

USP3 inhibits DDX58. 8 / 10

| 7 1

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"In particular, USP3, USP21, USP25 and USP15 have all been shown to directly inhibit RIG-I."

28295214

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sparser

"While the deubiquitinase USP3 inhibits MDA5 as well as RIG-I, it is thought that this may be due to USP3 directly binding the MDA5 CARD domain to prevent RNA filamentation (284)."

31379819

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➶

reach

"Our findings identify a previously unrecognized role of USP3 in RIG-I activation and provide insights into the mechanisms by which USP3 inhibits RIG-I signaling and antiviral immunity."

24366338

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➶

reach

"USP3, USP21 and CYLD inhibit RIG-I K63-linked ubiquitination and activation."

28744851

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reach

"Conversely, the removal of Lys63-linked ubiquitylation by the cellular deubiquitylating enzymes (DUBs) ubiquitin C-terminal hydrolase 3 (USP3), USP21 and CYLD, represses RIG-I signalling (reviewed in Ref."

27174148

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➶

reach

"While the deubiquitinase USP3 inhibits MDA5 as well as RIG-I, it is thought that this may be due to USP3 directly binding the MDA5 CARD domain to prevent RNA filamentation (284)."

31379819

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➶

reach

"The deubiquitinases USP3 and USP21 inhibit RIG-I activity by removing K63-linked ubiquitin chains."

| PMC

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➶

reach

"Conversely, the removal of Lys63 linked ubiquitylation by the cellular deubiquitylating enzymes (DUBs) ubiquitin C-terminal hydrolase 3 (USP3), USP21 and CYLD, represses RIG-I signalling."

27174148

USP3 deubiquitinates DDX58.

1 | 3

USP3 deubiquitinates DDX58. 4 / 4

1 | 3

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"CYLD (cylindromatosis) deubiquitinates RIG-I and several downstream molecules to prevent premature RIG-I activation in uninfected cells [17], while USP3 deubiquitinates RIG-I specifically after viral infection, likely serving as a negative feedback regulator [18]."

25644461

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ubibrowser

"USP3 inhibits type I interferon signaling by deubiquitinating RIG-I-like receptors"

24366338

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reach

"CYLD (cylindromatosis) deubiquitinates RIG-I and several downstream molecules to prevent premature RIG-I activation in uninfected cells [XREF_BIBR], while USP3 deubiquitinates RIG-I specifically after viral infection, likely serving as a negative feedback regulator [XREF_BIBR]."

25644461

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reach

"USP3 is also a DUB that deubiquitinates K63-polyUb chain of both RIG-I and MDA5 and suppresses IFN-β activation [25]."

31208841

USP3 ubiquitinates DDX58.

| 1

USP3 ubiquitinates DDX58. 1 / 1

| 1

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reach

"To the best of our knowledge, at least nine DUBs, A20, CYLD, USP3, USP5, USP14, USP15, USP21, USP25, and USP27X, have been proposed to counteract the K63linked ubiquitination of RIG-I and, thereby attenuate downstream signaling and IFN-b production ( Table 1 and Figure 3 ) (58, 76, 93) ."

34707613

USP3 activates DDX58.

| 1

USP3 activates DDX58. 1 / 1

| 1

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reach

"Ubiquitylation events are reversible processes, and, accordingly, several deubiquitylating enzymes, in particular ubiquitin specific peptidase 3 (USP3), USP21 and CYLD lysine 63 deubiquitinase (CYLD), modulate RIG-I signalling by removing K63-polyubiquitin chains, although with unique kinetics."

31792373

USP3 affects AS1

| 26

USP3 binds AS1. 10 / 21

| 21

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"Of these, RFPL1S ( p = 0.0001), PPP1R26-AS1 ( p = 0.03), RP11-439E19.3 ( p = 1.24 × 10 –6 ), CASC15 ( p = 1.6 × 10 –7 ), AC004540.5 ( p = 0.0002), and CTD-2881E23.2 ( p = 1.6 × 10 –9 ) were found to be significantly upregulated ( xref ), while USP3-AS1 ( p = 1.78 × 10 –6 ), CHRM3-AS2 ( p = 0.003) and RP6-99M1.2 ( p = 0.002) were significantly downregulated ( xref )."

31920530

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sparser

"Of these, one was upregulated, PPP1R26-AS1 ( p = 5.2 × 10 –4 , number of samples with high expression = 14, number of samples with low expression = 74), and the other was downregulated, USP3-AS1 ( p = 0.019, number of samples with high expression = 42, number of samples with low expression = 46) ( xref )."

31920530

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➶

sparser

"USP3-AS1 is a negative regulator of mitophagy and the regulatory effect is USP30-independent."

34653676

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sparser

"After cross-sectional analysis, three lncRNAs, CASC15 , PPP1R26-AS1 and USP3-AS1 , were found to be significantly altered in different datasets compared to other lncRNAs ( xref )."

31920530

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sparser

"In our study, the expression of MALAT1, USP3-AS1, and LINC-PINT was associated with worse overall survival in breast cancer patients."

33495450

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sparser

"USP3-AS1 has also been reported as a potential biomarker for neuroblastoma pathogenesis studies xref ."

33495450

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sparser

"b Overall survival (OS) as a function of MALAT1, USP3-AS1, and LINC-PINT expression in breast cancer."

33495450

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sparser

"This database revealed that the expression of three lncRNAs was altered significantly in neuroblastoma samples; two were upregulated, namely CASC15 ( p < 0.0001, unpaired two-tailed t -test with Welch’s correction) and PPP1R26-AS1 ( p < 0.05, unpaired two-tailed t -test with Welch’s correction), and one was downregulated, USP3-AS1 ( p < 0.0001, unpaired two-tailed t -test with Welch’s correction) ( xref )."

31920530

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sparser

"Interestingly, the PPP1R26-AS1- associated gene PPP1R26 was differentially expressed in all of the conditions [Tumor vs. MNCs, DTCs vs. MNCs, and DTCs (relapsed) vs. MNCs (relapsed)], whereas USP3-AS1 -associated coding genes USP3 and FBXL22 were differentially expressed only in primary neuroblastoma tumor."

31920530

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sparser

"Interestingly, the expression of MALAT1, USP3-AS1, and LINC-PINT was associated with worse overall survival in breast cancer patients (Fig. xref )."

33495450

USP3 binds MUC20, OXT, and AS1. 5 / 5

| 5

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"We suggest a possible reciprocal relationship between MALAT1 and each of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5."

33495450

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sparser

"Our data showed strong positive correlations between MALAT1 expression and the expression of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5 (Fig. xref ), highlighting a plausible reciprocal relationship between MALAT1 and the identified lncRNAs in TNBC."

33495450

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sparser

"Fig. 7: a Correlation between MALAT1 expression and the expression of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5 in 1104 BC patients from the StarBase database."

33495450

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sparser

"a Correlation between MALAT1 expression and the expression of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5 in 1104 BC patients from the StarBase database."

33495450

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sparser

"Our data showed strong positive correlations between MALAT1 expression and the expression of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5 (Fig. 7a), highlighting a plausible reciprocal relationship between MALAT1 and the identified lncRNAs in TNBC."

33495450

USP3 affects cell population proliferation

| 13

USP3 activates cell population proliferation.

| 11

USP3 activates cell population proliferation. 10 / 11

| 11

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reach

"USP3 knockdown inhibits breast cancer cell proliferation in vitro and tumorigenesis in vivo, which can be partially rescued by ectopic expression of KLF5."

31624151

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reach

"Cellular experiments confirmed that high expression of USP3 and Aurora A in ESCC cells promoted malignant cell proliferation and invasion."

34758762

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reach

"Upregulation of USP3 or downregulation of miR-224-5p restored proliferation and migration by MKN-28 and MGC-803 cells after hsa_circ_0017639 silencing."

32376451

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reach

"Therefore, USP3 might accelerate the proliferation of NSCLC cells via regulating RBM4."

32271432

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reach

"USP3 promotes proliferation of non small cell lung cancer through regulating RBM4."

32271432

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reach

"In vivo deletion of USP3, a deubiquitinating enzyme involved in DNA damage repair, increases the incidence of spontaneous cancer and impairs the proliferation and repopulation ability of HSCs."

25113974

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➶

reach

"Silencing of USP3 suppressed GC cell proliferation and spreading in vitro as well as xenograft proliferation and metastasis in vivo; however, opposite results were obtained when USP3 was overexpressed."

30168892

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➶

reach

"Upregulation of USP3 restored MKN-28 and MGC-803 cell proliferation and migration after overexpression of miR-224-5p."

32376451

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reach

"Here, we report that ubiquitin specific peptidase 3 (USP3) promotes proliferation and metastasis of esophageal squamous cell carcinoma (ESCC) cells by mediating deubiquitination of Aurora A. Analysis of human clinical samples indicated that USP3 and Aurora A are highly expressed in ESCC."

34758762

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➶

reach

"Protein deubiquitylase USP3 stabilizes Aurora A to promote proliferation and metastasis of esophageal squamous cell carcinoma."

34758762

USP3 inhibits cell population proliferation.

| 2

USP3 inhibits cell population proliferation. 2 / 3

| 2

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reach

"Additionally, overexpressed USP3 significantly promoted cell proliferation in vitro and tumor growth in vivo, while the silencing of USP3 inhibited proliferation and tumor growth."

36792297

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reach

"Depletion of tyrosine tRNA GUA or its translationally regulated targets USP3 and SCD repressed proliferation revealing a dedicated tRNA regulated growth-suppressive pathway for oxidative stress response."

33346941

USP3 affects IFIH1

2 | 4 7

USP3 binds IFIH1.

2 | 1 6

USP3 binds IFIH1. 3 / 3

2 | 1

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reach

"USP3 may bind to MDA5 via unanchored polyUb chains and then deubiquitinate it [XREF_BIBR]."

33138315

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➶

biogrid

No evidence text available

24366338

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➶

biogrid

No evidence text available

24366338

USP3 binds IFIH1 and DDX58. 3 / 3

| 3

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➶

sparser

"The interaction of USP3 with RIG-I and MDA5 is strictly based upon ligand stimulation."

33138315

---

➶

sparser

"Also co-IP demonstrated the interaction between USP3 and stimulated RIG-I or MDA5, but not the unstimulated ones, supporting that ligand stimulation is required for USP3 to interact with RIG-I or MDA5 [ xref ]."

31208841

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➶

sparser

"Also co-IP demonstrated the interaction between USP3 and stimulated RIG-I or MDA5, but not the unstimulated ones, supporting that ligand stimulation is required for USP3 to interact with RIG-I or MDA5 [25]."

31208841

USP3 binds IFIH1 and carD. 1 / 1

| 1

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➶

sparser

"While the deubiquitinase USP3 inhibits MDA5 as well as RIG-I, it is thought that this may be due to USP3 directly binding the MDA5 CARD domain to prevent RNA filamentation (284)."

31379819

USP3 binds IFIH1, K63, and polyubiquitin. 1 / 1

| 1

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➶

sparser

"In addition to RIG-I, USP3 also binds MDA5 to remove K63-polyubiquitin chains on the N-terminal CARDs [ xref ]."

28295214

USP3 binds IFIH1 and polyubiquitin. 1 / 1

| 1

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➶

sparser

"USP3 may bind to MDA5 via unanchored polyUb chains and then deubiquitinate it [ xref ]."

33138315

USP3 inhibits IFIH1.

| 2 1

USP3 inhibits IFIH1. 3 / 3

| 2 1

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➶

reach

"While the deubiquitinase USP3 inhibits MDA5 as well as RIG-I, it is thought that this may be due to USP3 directly binding the MDA5 CARD domain to prevent RNA filamentation (284)."

31379819

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➶

reach

"USP3 and USP21 inhibit MDA5 function via deubiquitination XREF_BIBR, XREF_BIBR, and proteins such as dihydroxyacetone kinase (DAK), Atg5-Atg-12, NLRC5, and TRIM13 interact with and inhibit MDA5 XREF_BIBR, XREF_BIBR - XREF_BIBR."

31827068

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➶

sparser

"While the deubiquitinase USP3 inhibits MDA5 as well as RIG-I, it is thought that this may be due to USP3 directly binding the MDA5 CARD domain to prevent RNA filamentation (284)."

31379819

USP3 deubiquitinates IFIH1.

| 1

USP3 deubiquitinates IFIH1. 1 / 1

| 1

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➶

reach

"USP3 is also a DUB that deubiquitinates K63-polyUb chain of both RIG-I and MDA5 and suppresses IFN-β activation [25]."

31208841

DDX58 affects USP3

2 | 5 5

USP3 binds DDX58. 7 / 7

2 | 5

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➶

biogrid

No evidence text available

24366338

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➶

reach

"Because it was recently shown that USP3 interacts with RIG-I to deubiquitinate K63 linked poly-ubiquitin chains on RIG-I and inhibit type I IFN pathways [XREF_BIBR], we focused on SRSF1 for further experiments."

25658361

---

➶

reach

"We found that USP3 and a splicing-factor oncoprotein, serine/arginine rich splicing factor 1 (SRSF1), interact with RIG-I."

25658361

---

➶

biogrid

No evidence text available

24366338

---

➶

reach

"Coimmunoprecipitation and western blot analyses revealed that USP3 and SRSF1 specifically interact with RIG-I in vitro (XREF_FIG and XREF_SUPPLEMENTARY)."

25658361

---

➶

reach

"USP3 binds RIG-I and removes the K63-polyubiquitin chains on the N-terminal CARDs [XREF_BIBR]."

28295214

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➶

reach

"Upon virus infection, USP3 interacted with RIG-I, likely acting as a negative feedback regulator."

25753787

USP3 binds IFIH1 and DDX58. 3 / 3

| 3

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➶

sparser

"The interaction of USP3 with RIG-I and MDA5 is strictly based upon ligand stimulation."

33138315

---

➶

sparser

"Also co-IP demonstrated the interaction between USP3 and stimulated RIG-I or MDA5, but not the unstimulated ones, supporting that ligand stimulation is required for USP3 to interact with RIG-I or MDA5 [ xref ]."

31208841

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➶

sparser

"Also co-IP demonstrated the interaction between USP3 and stimulated RIG-I or MDA5, but not the unstimulated ones, supporting that ligand stimulation is required for USP3 to interact with RIG-I or MDA5 [25]."

31208841

UBC binds USP3 and DDX58. 1 / 1

| 1

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➶

sparser

"Because it was recently shown that USP3 interacts with RIG-I to deubiquitinate K63-linked poly-ubiquitin chains on RIG-I and inhibit type I IFN pathways [ xref ], we focused on SRSF1 for further experiments."

25658361

SRSF1 binds USP3 and DDX58. 1 / 1

| 1

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➶

sparser

"To examine which proteins directly interact with RIG-I, we transfected 293T cells with HA-tagged candidate genes together with Flag-tagged RIG-I. Coimmunoprecipitation and western blot analyses revealed that USP3 and SRSF1 specifically interact with RIG-I in vitro ( xref and xref )."

25658361

USP3 affects RNF168

| 9

USP3 inhibits RNF168. 9 / 11

| 9

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"Similar to USP26 and USP37, two other DUBs, USP3 and USP44, were shown to reverse RNF168 induced chromatin ubiquitylation, thereby controlling the accumulation of BRCA1 and 53BP1 at sites of DNA damage."

26101254

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reach

"By virtue of its ability to oppose histone H2A ubiquitylation and the fact that USP3 over-expression can block accumulation of the ubiquitin E3 ligase RNF168 at DSB sites, USP3 has been linked to the key RNF8-RNF168 pathway of assembling repair and signalling components at DSB sites [XREF_BIBR]."

23712866

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➶

reach

"The DUBs USP3, USP16, BRCC36, POH1, and OTUB1 are associated with negative regulation of the RNF8 pathway, with USP3 and USP16 being first linked to this pathway through their ability to oppose H2A ub[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

23416108

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reach

"In this context, overexpression of wild-type USP3, but not its catalytically inactive mutant, prevented the IR induced focus formation of RNF168, emphasizing the requirement of UPS3 cysteine protease activity to reverse the DSB ubiquitin response at the level of RNF8 XREF_BIBR."

21056014

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➶

reach

"USP3: Antagonizing RNF8/RNF168 at H2A-Type Histones."

32708614

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➶

reach

"Overexpression of USP3, a deubiquitylating enzyme that reversed H2A ubiquitylation, as well as mutations in the UIM domains of RNF168, prevented the accumulation of RNF168 at DSBs."

19342239

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➶

reach

"By virtue of its ability to oppose histone H2A ubiquitylation and the fact that USP3 over-expression can block accumulation of the ubiquitin E3 ligase RNF168 at DSB sites, USP3 has been linked to the key RNF8–RNF168 pathway of assembling repair and signalling components at DSB sites [84]."

23712866

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➶

reach

"In this context, overexpression of wild-type USP3, but not its catalytically inactive mutant, prevented the IR induced focus formation of RNF168, emphasizing the requirement of UPS3 cysteine protease [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

21056014

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➶

reach

"But most significantly in this context, overexpression of wild-type USP3, but not its catalytically inactive mutant, prevented the IR induced focus formation of RNF168, indicating that the assembly of[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

19203579

USP3 affects Neoplasm Metastasis

| 3 8

USP3 activates Neoplasm Metastasis.

| 3 6

USP3 activates Neoplasm Metastasis. 9 / 9

| 3 6

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➶

eidos

"These data suggest that USP3 promotes GC progression and metastasis by deubiquitinating COL9A3 and COL6A5 ."

34930901

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➶

reach

"Here, we report that ubiquitin specific peptidase 3 (USP3) promotes proliferation and metastasis of esophageal squamous cell carcinoma (ESCC) cells by mediating deubiquitination of Aurora A. Analysis of human clinical samples indicated that USP3 and Aurora A are highly expressed in ESCC."

34758762

---

➶

reach

"Silencing of USP3 suppressed GC cell proliferation and spreading in vitro as well as xenograft proliferation and metastasis in vivo; however, opposite results were obtained when USP3 was overexpressed."

30168892

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➶

reach

"USP3 knockdown induced metastasis was abolished by USP3 3 ' UTR overexpression in CRC cells."

28655924

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➶

eidos

"Hsa_circ_0017639 expression promotes gastric cancer proliferation and metastasis by sponging miR-224-5p and upregulating USP3 ."

32376451

---

➶

eidos

"USP3 promotes gastric cancer progression and metastasis by deubiquitination-dependent COL9A3 / COL6A5 stabilisation ."

34930901

---

➶

reach

"USP3 promotes gastric cancer progression and metastasis by deubiquitination-dependent COL9A3/COL6A5 stabilisation."

34930901

---

➶

reach

"However, the mechanism by which USP3 promotes gastric cancer (GC) metastasis remains largely unknown."

31234902

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➶

reach

"Protein deubiquitylase USP3 stabilizes Aurora A to promote proliferation and metastasis of esophageal squamous cell carcinoma."

34758762

USP3 inhibits Neoplasm Metastasis.

| 2

USP3 inhibits Neoplasm Metastasis. 2 / 2

| 2

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➶

reach

"USP3 and SMAD4 were directly targeted by miR-224, and overexpression of the USP3 3 ' UTR could inhibit metastasis caused by the loss of USP3."

28655924

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➶

reach

"These results indicated that the loss of USP3 facilitated tumour progression, while overexpression of the USP3 3 ' UTR inhibited metastasis in CRC cells."

28655924

USP3 affects Ubiquitin

| 1 8

USP3 inhibits Ubiquitin.

| 1 3

USP3 inhibits Ubiquitin. 4 / 5

| 1 3

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➶

reach

"By virtue of its ability to oppose histone H2A ubiquitylation and the fact that USP3 over-expression can block accumulation of the ubiquitin E3 ligase RNF168 at DSB sites, USP3 has been linked to the key RNF8–RNF168 pathway of assembling repair and signalling components at DSB sites [84]."

23712866

---

➶

reach

"The Ub modifications on K119/120 of histone H2A and K13/15 of histone H2B generated by BMI1 and RNF2 and RNF20-RNF40 are attenuated by USP3, an H2A and H2B specific DUB."

28587922

---

➶

reach

"By virtue of its ability to oppose histone H2A ubiquitylation and the fact that USP3 over-expression can block accumulation of the ubiquitin E3 ligase RNF168 at DSB sites, USP3 has been linked to the key RNF8-RNF168 pathway of assembling repair and signalling components at DSB sites [XREF_BIBR]."

23712866

---

➶

trips

"We further identified that the USP3 removes Ub at lysine 13 and 15 of H2A and γH2AX, as well as lysine 118 and 119 of H2AX in response to DNA damage."

24196443

USP3 activates Ubiquitin.

| 5

USP3 activates Ubiquitin. 5 / 5

| 5

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➶

reach

"The data relate to the research article " Tight regulation of ubiquitin mediated DNA damage response by USP3 preserves the functional integrity of hematopoietic stem cells " (Lancini et al., 2014) XREF_BIBR."

26909367

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➶

reach

"The USP3 protein is a functional ubiquitin specific protease in vitro, and is able to inhibit ubiquitin dependent degradation of both an N-end Rule substrate and abnormal endogenous proteins in yeast."

10480896

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➶

reach

"The data relate to the research article " Tight regulation of ubiquitin mediated DNA damage response by USP3 preserves the functional integrity of hematopoietic stem cells " (Lancini et al., 2014) [4]."

26909367

---

➶

reach

"Paradoxically, by removing the bulky ubiquitin chain at the active site, USP3 also increased the accessibility of CHK1 to its substrates."

29735693

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➶

reach

"Tight regulation of ubiquitin mediated DNA damage response by USP3 preserves the functional integrity of hematopoietic stem cells."

25113974

IFIH1 affects USP3

2 | 2 6

IFIH1 binds USP3.

2 | 1 6

USP3 binds IFIH1. 3 / 3

2 | 1

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➶

reach

"USP3 may bind to MDA5 via unanchored polyUb chains and then deubiquitinate it [XREF_BIBR]."

33138315

---

➶

biogrid

No evidence text available

24366338

---

➶

biogrid

No evidence text available

24366338

USP3 binds IFIH1 and DDX58. 3 / 3

| 3

---

➶

sparser

"The interaction of USP3 with RIG-I and MDA5 is strictly based upon ligand stimulation."

33138315

---

➶

sparser

"Also co-IP demonstrated the interaction between USP3 and stimulated RIG-I or MDA5, but not the unstimulated ones, supporting that ligand stimulation is required for USP3 to interact with RIG-I or MDA5 [ xref ]."

31208841

---

➶

sparser

"Also co-IP demonstrated the interaction between USP3 and stimulated RIG-I or MDA5, but not the unstimulated ones, supporting that ligand stimulation is required for USP3 to interact with RIG-I or MDA5 [25]."

31208841

USP3 binds IFIH1 and carD. 1 / 1

| 1

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➶

sparser

"While the deubiquitinase USP3 inhibits MDA5 as well as RIG-I, it is thought that this may be due to USP3 directly binding the MDA5 CARD domain to prevent RNA filamentation (284)."

31379819

USP3 binds IFIH1, K63, and polyubiquitin. 1 / 1

| 1

---

➶

sparser

"In addition to RIG-I, USP3 also binds MDA5 to remove K63-polyubiquitin chains on the N-terminal CARDs [ xref ]."

28295214

USP3 binds IFIH1 and polyubiquitin. 1 / 1

| 1

---

➶

sparser

"USP3 may bind to MDA5 via unanchored polyUb chains and then deubiquitinate it [ xref ]."

33138315

IFIH1 inhibits USP3.

| 1

IFIH1 inhibits USP3. 1 / 1

| 1

---

➶

reach

"Notably, USP3 was shown to directly bind the MDA5 CARDs and inhibited signaling by the constitutively active, truncated form of MDA5 (N-MDA5)."

28295214

USP3 affects Histone

| 8 1

USP3 deubiquitinates Histone.

| 6

USP3 deubiquitinates Histone. 3 / 3

| 3

---

➶

reach

"Notably, USP3 deletion increased the levels of histone ubiquitination in adult tissues, reduced the hematopoietic stem cell (HSC) reserves over time, and shortened animal life span."

25113974

---

➶

reach

"Consistently, we found that USP3, which deubiquitylates H2A-type histones to prevent accumulation of RNF168 and downstream repair factors, but not RNF8, at DSB sites, also suppressed RNF169 recruitment in a manner requiring its catalytic activity (XREF_FIG)."

22492721

---

➶

reach

"Overexpression of USP3 in HeLa cells reduced levels of both Ub-H2A and Ub-H2B, whereas knockdown of USP3 enhanced the ubiquitination of both histones."

18226187

USP3 deubiquitinates Histone-H2A. 3 / 3

| 3

---

➶

reach

"RNF168 is an E3 ligase that ubiquitinates histones H2A and gammaH2AX during the DNA damage response [XREF_BIBR], this ubiquitination can be reversed by Usp3 [XREF_BIBR]."

25962961

---

➶

reach

"Deubiquitination of histones H2A and H2B by USP3 is required for progression through the S phase and for genomic stability."

22941662

---

➶

reach

"Several DUBs have been implicated in histone deubiquitination, including USP3, USP12, USP22, and USP46, which deubiquitinate both histones H2A and histones H2B [XREF_BIBR]."

33327527

USP3 ubiquitinates Histone.

| 2

USP3 leads to the ubiquitination of Histone on K13. 1 / 1

| 1

---

➶

reach

"Irradiated USP3-knockdown cells showed prolonged accumulation of ubiquitinated histone H2A (Mosbech et al., 2013; Nicassio et al., 2007), while over-expression of a wild type but not catalytically ina[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

24647359

USP3 leads to the ubiquitination of Histone on K15. 1 / 1

| 1

---

➶

reach

"Irradiated USP3-knockdown cells showed prolonged accumulation of ubiquitinated histone H2A (Mosbech et al., 2013; Nicassio et al., 2007), while over-expression of a wild type but not catalytically ina[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

24647359

USP3 binds Histone.

| 1

Histone binds USP3. 1 / 1

| 1

---

➶

sparser

"USP3 does in fact interact with ubiquitinated H2A-type histones, and mouse embryonic fibroblasts isolated from USP3-null mice express increased levels of ubiquitinated H2A [ xref ]."

32708614

USP3 affects SUZ12

1 | 1 6

USP3 binds SUZ12.

| 1 6

USP3 binds SUZ12. 7 / 7

| 1 6

---

➶

sparser

"To further confirm that USP3 was associated with the SUZ12 protein, we performed reciprocal co-immunoprecipitation experiments using endogenous protein and showed that USP3 can interact with SUZ12 (Fig. xref )."

31234902

---

➶

sparser

"Thus, targeting the USP3-SUZ12 axis may provide a potential therapeutic target for the treatment of GC."

31234902

---

➶

sparser

"Taken together, these data suggest that the USP3-SUZ12 axis promotes an EMT-like phenotype in GC cells."

31234902

---

➶

sparser

"The USP3-SUZ12 axis might promote tumor progression and could be a potential therapeutic candidate for human GC."

31234902

---

➶

sparser

"Collectively, these findings suggest that USP3 can physically interact with SUZ12."

31234902

---

➶

sparser

"These findings demonstrate that USP3 is an essential regulator of SUZ12 and that the USP3-SUZ12 signaling axis plays a critical role in the progression and metastasis of GC."

31234902

---

➶

reach

"We observed that USP3 interacted with and stabilized SUZ12 via deubiquitination."

31234902

USP3 deubiquitinates SUZ12.

1 |

USP3 deubiquitinates SUZ12. 1 / 1

1 |

---

➶

ubibrowser

"We observed that USP3 interacted with and stabilized SUZ12 via deubiquitination."

31234902

USP3 affects IFNB1

| 8

USP3 inhibits IFNB1.

| 4

USP3 inhibits IFNB1. 4 / 4

| 4

---

➶

reach

"Cellular ubiquitin specific proteases, USP21, USP3 and USP15, a subfamily of deubiqutinase, remove K63 linked polyubiquitin chains from RIG-I and block it to induce IFN-beta."

28286234

---

➶

reach

"Ectopic expression of USP3 inhibited IFN-beta promoter activity in cells treated with low molecular weight (LMW) poly (I : C), and in cells infected with VSV."

28295214

---

➶

reach

"Ectopic expression of USP3 inhibited IFN-beta promoter activity in cells treated with high molecular weight (HMW) poly (I : C), whereas gene silencing of USP3 enhanced IFN-beta protein expression under similar treatment."

28295214

---

➶

reach

"The ectopic expression of USP3 in HEK293T cells that are treated with low molecular weight poly (I : C) and THP-1 cells infected with VSV attenuated the IFN-beta promoter activity."

33138315

USP3 decreases the amount of IFNB1.

| 3

USP3 decreases the amount of IFNB1. 3 / 3

| 3

---

➶

reach

"Ectopic expression of USP3 inhibited IFN-beta promoter activity in cells treated with high molecular weight (HMW) poly (I : C), whereas gene silencing of USP3 enhanced IFN-beta protein expression under similar treatment."

28295214

---

➶

reach

"Gene silencing of USP3 enhanced IFN-beta protein expression under similar treatments."

28295214

---

➶

reach

"Conversely, the silencing of USP3 enhanced the phosphorylation of IRF3 and IFN-beta protein expression under similar conditions."

33138315

USP3 dephosphorylates IFNB1.

| 1

USP3 leads to the dephosphorylation of IFNB1. 1 / 1

| 1

---

➶

reach

"Conversely, the silencing of USP3 enhanced the phosphorylation of IRF3 and IFN-beta protein expression under similar conditions."

33138315

SUZ12 affects USP3

| 2 6

SUZ12 binds USP3.

| 1 6

USP3 binds SUZ12. 7 / 7

| 1 6

---

➶

sparser

"To further confirm that USP3 was associated with the SUZ12 protein, we performed reciprocal co-immunoprecipitation experiments using endogenous protein and showed that USP3 can interact with SUZ12 (Fig. xref )."

31234902

---

➶

sparser

"Thus, targeting the USP3-SUZ12 axis may provide a potential therapeutic target for the treatment of GC."

31234902

---

➶

sparser

"Taken together, these data suggest that the USP3-SUZ12 axis promotes an EMT-like phenotype in GC cells."

31234902

---

➶

sparser

"The USP3-SUZ12 axis might promote tumor progression and could be a potential therapeutic candidate for human GC."

31234902

---

➶

sparser

"Collectively, these findings suggest that USP3 can physically interact with SUZ12."

31234902

---

➶

sparser

"These findings demonstrate that USP3 is an essential regulator of SUZ12 and that the USP3-SUZ12 signaling axis plays a critical role in the progression and metastasis of GC."

31234902

---

➶

reach

"We observed that USP3 interacted with and stabilized SUZ12 via deubiquitination."

31234902

SUZ12 activates USP3.

| 1

SUZ12 activates USP3. 1 / 1

| 1

---

➶

reach

"SUZ12 knockdown inhibited USP3 induced migration and invasion, as well as EMT in GC cells."

31234902

USP3 affects POU5F1

| 1 6

USP3 binds POU5F1. 7 / 7

| 1 6

---

➶

sparser

"USP3 interacts with endogenous Oct4 and co-localizes in the nucleus of hESCs."

34070420

---

➶

sparser

"USP3 Interacted with Oct4."

34070420

---

➶

sparser

"For this purpose, we performed immunoprecipitation (IP) experiments to investigate the interaction between USP3 and Oct4 at the exogenous level."

34070420

---

➶

sparser

"IP using endogenous USP3 antibody resulted in precipitation of endogenous Oct4 in NCCIT cells ( xref C) as well as in hiPSCs ( xref D), indicating that USP3 interacts stably with endogenous Oct4 in stem cells."

34070420

---

➶

reach

"USP3 interacts with endogenous Oct4 and co-localizes in the nucleus of hESCs."

34070420

---

➶

sparser

"Furthermore, we demonstrated that USP3 and Oct4 interact regardless of whether Oct4 is endogenously or exogenously expressed and co-localize in the nuclei of hESCs ( xref )."

34070420

---

➶

sparser

"Thus, the interaction between USP3 and Oct4 is critical for maintenance of the pluripotency of hESCs."

34070420

USP3 affects Histone_H2B

| 7

USP3 deubiquitinates Histone_H2B.

| 6

USP3 deubiquitinates Histone_H2B. 6 / 6

| 6

---

➶

reach

"Usp3 can deubiquitinate both H2A and H2B in humans."

19489724

---

➶

reach

"In the DDR, USP3 dynamically interacts with chromatin and deubiquitinates H2A and H2B."

26882840

---

➶

reach

"In addition to USP22 and its yeast homolog, H2B can be deubiquitylated by USP3 and USP7 in humans."

21513801

---

➶

reach

"USP3 that was found as chromatin associated DUB suppresses global mono-ubiquitylation of H2A (and also H2B)."

28764946

---

➶

reach

"USP3 dynamically associates with chromatin and deubiquitinates H2A and H2B in vivo."

17980597

---

➶

reach

"Deubiquitination of histones H2A and H2B by USP3 is required for progression through the S phase and for genomic stability."

22941662

USP3 binds Histone_H2B.

| 1

Histone_H2B binds USP3. 1 / 1

| 1

---

➶

reach

"In the DDR, USP3 dynamically interacts with chromatin and deubiquitinates H2A and H2B."

26882840

POU5F1 affects USP3

| 1 6

USP3 binds POU5F1. 7 / 7

| 1 6

---

➶

sparser

"USP3 interacts with endogenous Oct4 and co-localizes in the nucleus of hESCs."

34070420

---

➶

sparser

"USP3 Interacted with Oct4."

34070420

---

➶

sparser

"For this purpose, we performed immunoprecipitation (IP) experiments to investigate the interaction between USP3 and Oct4 at the exogenous level."

34070420

---

➶

sparser

"IP using endogenous USP3 antibody resulted in precipitation of endogenous Oct4 in NCCIT cells ( xref C) as well as in hiPSCs ( xref D), indicating that USP3 interacts stably with endogenous Oct4 in stem cells."

34070420

---

➶

reach

"USP3 interacts with endogenous Oct4 and co-localizes in the nucleus of hESCs."

34070420

---

➶

sparser

"Furthermore, we demonstrated that USP3 and Oct4 interact regardless of whether Oct4 is endogenously or exogenously expressed and co-localize in the nuclei of hESCs ( xref )."

34070420

---

➶

sparser

"Thus, the interaction between USP3 and Oct4 is critical for maintenance of the pluripotency of hESCs."

34070420

JUN affects USP3

7 |

JUN decreases the amount of USP3. 7 / 7

7 |

---

➶

biopax:msigdb

No evidence text available

---

➶

biopax:msigdb

No evidence text available

---

➶

biopax:msigdb

No evidence text available

---

➶

biopax:msigdb

No evidence text available

---

➶

biopax:msigdb

No evidence text available

---

➶

biopax:msigdb

No evidence text available

---

➶

biopax:msigdb

No evidence text available

USP3 affects IRF3

| 1 3 2

USP3 inhibits IRF3.

| 1 2

USP3 inhibits IRF3. 3 / 3

| 1 2

---

➶

eidos

"USP3 did not inhibit MAVS , STING , TBK1 , IRF3 and TIRF , as demonstrated by ISRE-luc activity induction test [ 25 ] ."

31208841

---

➶

sparser

"USP3 did not inhibit MAVS, STING, TBK1, IRF3 and TIRF, as demonstrated by ISRE-luc activity induction test [ xref ]."

31208841

---

➶

sparser

"USP3 did not inhibit MAVS, STING, TBK1, IRF3 and TIRF, as demonstrated by ISRE-luc activity induction test [25]."

31208841

USP3 phosphorylates IRF3.

| 1

USP3 leads to the phosphorylation of IRF3. 1 / 1

| 1

---

➶

reach

"Knockdown of USP3 specifically enhanced K63 linked ubiquitination of RIG-I, upregulated the phosphorylation of IRF3 and augmented the production of type I interferon cytokines and antiviral immunity."

24366338

USP3 dephosphorylates IRF3.

| 1

USP3 leads to the dephosphorylation of IRF3. 1 / 1

| 1

---

➶

reach

"Conversely, the silencing of USP3 enhanced the phosphorylation of IRF3 and IFN-beta protein expression under similar conditions."

33138315

USP3 decreases the amount of IRF3.

| 1

USP3 decreases the amount of IRF3. 1 / 1

| 1

---

➶

reach

"Conversely, the silencing of USP3 enhanced the phosphorylation of IRF3 and IFN-beta protein expression under similar conditions."

33138315

USP3 affects TP53

1 | 1 1 2

USP3 binds TP53.

| 1 1 2

TP53 binds USP3. 4 / 4

| 1 1 2

---

➶

reach

"Here we report that USP3 interacts with p53 and regulates p53 stability."

28807825

---

➶

trips

"Here we report that USP3 interacts with p53 and regulates p53 stability."

28807825

---

➶

sparser

"Here we report that USP3 interacts with p53 and regulates p53 stability."

28807825

---

➶

sparser

"Fu S and his colleagues have shown that USP3 interacts with p53 and regulates p53 stability and that depletion of USP3 leads to accelerated degradation of p53 in normal cells and therefore enhanced cell proliferation and transformation [ xref ]."

31234902

USP3 deubiquitinates TP53.

1 |

USP3 deubiquitinates TP53. 1 / 1

1 |

---

➶

ubibrowser

"Depletion of USP3 lead to accelerated degradation of p53 in normal cells thereby enhanced cell proliferation and transformation."

28807825

USP3 affects SMAD4

| 1 4

USP3 increases the amount of SMAD4.

| 2

USP3 increases the amount of SMAD4. 2 / 2

| 2

---

➶

reach

"USP3 modulated SMAD4 expression at the post-transcriptional level."

28655924

---

➶

reach

"In the present study, we found that USP3 modulated SMAD4 expression in a miRNA dependent, and protein coding gene independent manner."

28655924

USP3 binds SMAD4.

| 2

USP3 binds SMAD4. 2 / 2

| 2

---

➶

reach

"We utilized luciferase reporter constructs of PGL3-USP3 3 ' UTR and PGL3-SMAD4 3 ' UTR to evaluate whether the interaction between USP3 and SMAD4 was 3 ' UTR dependent."

28655924

---

➶

reach

"This reversal was partially due to the ceRNA interaction between USP3 and SMAD4."

28655924

USP3 activates SMAD4.

| 1

USP3 activates SMAD4. 1 / 1

| 1

---

➶

eidos

"Wang et al. demonstrated that USP3 upregulated SMAD4 by competitively binding miR-224 , thereby inhibiting CRC cell metastasis [ 24 ] ."

34081622

USP3 affects OXT

| 5

USP3 binds MUC20, OXT, and AS1. 5 / 5

| 5

---

➶

sparser

"We suggest a possible reciprocal relationship between MALAT1 and each of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5."

33495450

---

➶

sparser

"Our data showed strong positive correlations between MALAT1 expression and the expression of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5 (Fig. xref ), highlighting a plausible reciprocal relationship between MALAT1 and the identified lncRNAs in TNBC."

33495450

---

➶

sparser

"Fig. 7: a Correlation between MALAT1 expression and the expression of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5 in 1104 BC patients from the StarBase database."

33495450

---

➶

sparser

"a Correlation between MALAT1 expression and the expression of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5 in 1104 BC patients from the StarBase database."

33495450

---

➶

sparser

"Our data showed strong positive correlations between MALAT1 expression and the expression of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5 (Fig. 7a), highlighting a plausible reciprocal relationship between MALAT1 and the identified lncRNAs in TNBC."

33495450

USP3 affects MAVS

| 1 2 2

USP3 inhibits MAVS. 5 / 5

| 1 2 2

---

➶

sparser

"USP3 did not inhibit MAVS, STING, TBK1, IRF3 and TIRF, as demonstrated by ISRE-luc activity induction test [ xref ]."

31208841

---

➶

sparser

"USP3 did not inhibit MAVS, STING, TBK1, IRF3 and TIRF, as demonstrated by ISRE-luc activity induction test [25]."

31208841

---

➶

reach

"Notably, USP3 inhibited signaling by the constitutively active, truncated form of RIG-I (N-RIG-I), but did not inhibit MAVS signaling."

28295214

---

➶

eidos

"USP3 did not inhibit MAVS , STING , TBK1 , IRF3 and TIRF , as demonstrated by ISRE-luc activity induction test [ 25 ] ."

31208841

---

➶

reach

"USP3 did not inhibit MAVS, STING, TBK1, IRF3 and TIRF, as demonstrated by ISRE-luc activity induction test [XREF_BIBR]."

31208841

USP3 affects KLF5

1 | 2 2

USP3 binds KLF5.

| 1 2

USP3 binds KLF5. 2 / 2

| 1 1

---

➶

sparser

"We demonstrated that USP3 interacts with KLF5 and stabilizes KLF5 via deubiquitination."

31624151

---

➶

reach

"We demonstrated that USP3 interacts with KLF5 and stabilizes KLF5 via deubiquitination."

31624151

USP3 binds KLF5 and BAP1. 1 / 1

| 1

---

➶

sparser

"Both BAP1 and USP3 bind to and stabilize KLF5 via deubiquitination [ xref , xref ], indicating the possible regulatory role of DUBs in cancer proliferation."

32268558

USP3 deubiquitinates KLF5.

1 | 1

USP3 leads to the deubiquitination of KLF5. 2 / 2

1 | 1

---

➶

reach

"USP3 promotes breast cancer cell proliferation by deubiquitinating KLF5."

31624151

---

➶

ubibrowser

"In this study, ubiquitin-specific protease 3 (USP3) was identified as a new KLF5 deubiquitinase by genome-wide siRNA library screening."

31624151

TP53 affects USP3

1 | 1 1 2

TP53 binds USP3.

| 1 1 2

TP53 binds USP3. 4 / 4

| 1 1 2

---

➶

reach

"Here we report that USP3 interacts with p53 and regulates p53 stability."

28807825

---

➶

trips

"Here we report that USP3 interacts with p53 and regulates p53 stability."

28807825

---

➶

sparser

"Here we report that USP3 interacts with p53 and regulates p53 stability."

28807825

---

➶

sparser

"Fu S and his colleagues have shown that USP3 interacts with p53 and regulates p53 stability and that depletion of USP3 leads to accelerated degradation of p53 in normal cells and therefore enhanced cell proliferation and transformation [ xref ]."

31234902

TP53 decreases the amount of USP3.

1 |

TP53 decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:msigdb

No evidence text available

OXT affects USP3

| 5

USP3 binds MUC20, OXT, and AS1. 5 / 5

| 5

---

➶

sparser

"We suggest a possible reciprocal relationship between MALAT1 and each of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5."

33495450

---

➶

sparser

"Our data showed strong positive correlations between MALAT1 expression and the expression of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5 (Fig. xref ), highlighting a plausible reciprocal relationship between MALAT1 and the identified lncRNAs in TNBC."

33495450

---

➶

sparser

"Fig. 7: a Correlation between MALAT1 expression and the expression of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5 in 1104 BC patients from the StarBase database."

33495450

---

➶

sparser

"a Correlation between MALAT1 expression and the expression of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5 in 1104 BC patients from the StarBase database."

33495450

---

➶

sparser

"Our data showed strong positive correlations between MALAT1 expression and the expression of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5 (Fig. 7a), highlighting a plausible reciprocal relationship between MALAT1 and the identified lncRNAs in TNBC."

33495450

Pirinixic acid affects USP3

4 |

Pirinixic acid increases the amount of USP3.

2 |

Pirinixic acid increases the amount of USP3. 2 / 2

2 |

---

➶

ctd

No evidence text available

17426115

---

➶

ctd

No evidence text available

23811191

Pirinixic acid decreases the amount of USP3.

1 |

Pirinixic acid decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

19710929

Pirinixic acid activates USP3.

1 |

Pirinixic acid activates USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

19710929

USP3 affects UBC

3 | 1

UBC binds USP3. 3 / 3

3 |

---

➶

biogrid

No evidence text available

17980597

---

➶

biogrid

No evidence text available

10480896

---

➶

biogrid

No evidence text available

28190767

UBC binds USP3 and DDX58. 1 / 1

| 1

---

➶

sparser

"Because it was recently shown that USP3 interacts with RIG-I to deubiquitinate K63-linked poly-ubiquitin chains on RIG-I and inhibit type I IFN pathways [ xref ], we focused on SRSF1 for further experiments."

25658361

USP3 affects TP53BP1

| 4

USP3 inhibits TP53BP1.

| 3

USP3 inhibits TP53BP1. 3 / 3

| 3

---

➶

reach

"53BP1 and RAP80 foci are also negatively regulated by USP3 catalytic activity [18,110,132,135,136]."

32708614

---

➶

reach

"In addition, USP3 overexpression impaired the accumulation of downstream repair factors BRCA1 and 53BP1 at the damage sites in response to both UV and gamma irradiation."

24196443

---

➶

reach

"Overexpression of USP3, while having no effect on retention of RNF8 at DSBs, abolished the IR induced focus formation of RNF168, RAP80, and 53BP1, indicating that it de-ubiquitylates RNF8 targeted substrates."

23847653

USP3 activates TP53BP1.

| 1

USP3 activates TP53BP1. 1 / 1

| 1

---

➶

reach

"Furthermore, although depletion of USP3 or RNF169, two established regulators of RNF168 controlled events (Doil et al., 2009; Poulsen et al., 2012), increased 53BP1 foci intensity, this effect was con[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

22884692

USP3 affects Stomach Neoplasms

| 3 1

USP3 activates Stomach Neoplasms. 4 / 4

| 3 1

---

➶

eidos

"These data suggest that USP3 promotes GC progression and metastasis by deubiquitinating COL9A3 and COL6A5 ."

34930901

---

➶

eidos

"USP3 promotes gastric cancer progression and metastasis by deubiquitination-dependent COL9A3 / COL6A5 stabilisation ."

34930901

---

➶

reach

"Silencing of USP3 suppressed GC cell proliferation and spreading in vitro as well as xenograft proliferation and metastasis in vivo; however, opposite results were obtained when USP3 was overexpressed."

30168892

---

➶

eidos

"Hsa_circ_0017639 expression promotes gastric cancer proliferation and metastasis by sponging miR-224-5p and upregulating USP3 ."

32376451

USP3 affects STING1

1 | 1 2

USP3 inhibits STING1.

| 1 2

USP3 inhibits STING1. 3 / 3

| 1 2

---

➶

sparser

"USP3 did not inhibit MAVS, STING, TBK1, IRF3 and TIRF, as demonstrated by ISRE-luc activity induction test [ xref ]."

31208841

---

➶

sparser

"USP3 did not inhibit MAVS, STING, TBK1, IRF3 and TIRF, as demonstrated by ISRE-luc activity induction test [25]."

31208841

---

➶

eidos

"USP3 did not inhibit MAVS , STING , TBK1 , IRF3 and TIRF , as demonstrated by ISRE-luc activity induction test [ 25 ] ."

31208841

USP3 binds STING1.

1 |

USP3 binds STING1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

27801882

USP3 affects SNAI1

| 3 1

USP3 binds SNAI1.

| 1 1

SNAI1 binds USP3. 2 / 2

| 1 1

---

➶

sparser

"USP3 interacts directly with Snail and stabilizes Snail via deubiquitination."

31266817

---

➶

reach

"USP3 interacts directly with Snail and stabilizes Snail via deubiquitination."

31266817

USP3 ubiquitinates SNAI1.

| 1

USP3 leads to the ubiquitination of SNAI1. 1 / 1

| 1

---

➶

reach

"USP3 mediates the deubiquitination of snail family transcriptional repressor 1 (SNAIL1)."

35739527

USP3 activates SNAI1.

| 1

USP3 activates SNAI1. 1 / 1

| 1

---

➶

reach

"Implications : Our study establishes USP3 mediated Snail stabilization as an important mechanism underlying GBM invasion and progression, and provides a rationale for potential therapeutic interventions in the treatment of GBM."

31266817

USP3 affects Interferon

| 1 3

USP3 inhibits Interferon.

| 1 2

USP3 inhibits Interferon. 3 / 3

| 1 2

---

➶

reach

"USP3 inhibits type I interferon signaling by deubiquitinating RIG-I-like receptors."

24366338

---

➶

eidos

"USP3 inhibits type I interferon signaling by deubiquitinating RIG-Ilike receptors ."

26712804

---

➶

reach

"USP3 also negatively regulates IFN signaling by interacting with RIG-I and MDA5, but not with other downstream signaling proteins like MAVS, TBK1, IKKi, IRF3, TRAF3, or TRAF6."

33138315

USP3 increases the amount of Interferon.

| 1

USP3 increases the amount of Interferon. 1 / 1

| 1

---

➶

reach

"Several other DUBs have also been implicated in the regulation of RIG-I ubiquitination and virus induced type I IFN expression; these include A20, USP3, USP15, USP21, and USP25 XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR."

27012466

USP3 affects His2A

| 4

USP3 inhibits His2A.

| 2

USP3 inhibits His2A. 2 / 2

| 2

---

➶

reach

"Overexpression of Usp3 in HeLa cells causes the reduction in both uH2A and uH2B without altering the bulk pool of ubiquitinated proteins."

19489724

---

➶

reach

"Only wild-type USP3 significantly reduced the total pool of uH2A, showing that both protease activity and an intact zinc finger are required for H2A deubiquitination."

17980597

USP3 decreases the amount of His2A.

| 1

Modified USP3 decreases the amount of His2A. 1 / 1

| 1

---

➶

reach

"Overexpression of USP3 in HeLa cells reduced levels of both Ub-H2A and Ub-H2B, whereas knockdown of USP3 enhanced the ubiquitination of both histones."

18226187

USP3 activates His2A.

| 1

USP3 activates His2A. 1 / 1

| 1

---

➶

reach

"For example, one could propose that USP3 might specifically target the pool of Ub-H2A that accumulates at DNA repair foci, or that MYSM1 and USP16 are recruited to different cohorts of gene promoters."

18226187

USP3 affects Flag

| 4

USP3 binds Flag. 4 / 4

| 4

---

➶

sparser

"Similarly, in HEK293 cells, we observed protein stabilization of ectopically expressed Myc-Oct4 as the concentration of Flag-USP3 increased ( xref E), but expression of the catalytic mutant Flag-USP3CS had no such stabilization effect ( xref F)."

34070420

---

➶

sparser

"We co-transfected Flag-USP3 and Myc-Oct4 into HEK293 cells and performed IP with Flag or Myc antibodies, followed by Western blot with reciprocal antibodies."

34070420

---

➶

sparser

"To determine differentially expressed proteins that may serve as potential interaction targets of USP3, total protein extracted from the treated BGC-823 cells stably expressing the Flag-USP3 fusion protein and the BGC-823 cells carrying an empty vector was analyzed using iTRAQ experiments."

31234902

---

➶

sparser

"Flag-USP3 co-precipitated Myc-Oct4 ( xref A), and similarly Myc-Oct4 co-precipitated Flag-USP3 ( xref B)."

34070420

USP3 affects COL6A5

| 2 2

USP3 binds COL6A5.

| 1 2

USP3 binds COL6A5. 3 / 3

| 1 2

---

➶

sparser

"This study suggests that targeting of the USP3-COL9A3 or USP3-COL6A5 axis could be developed as a therapeutic strategy for GC management."

34930901

---

➶

reach

"Mechanistically, we observed that USP3 interacted with and stabilised COL9A3 and COL6A5 via deubiquitination in GC."

34930901

---

➶

sparser

"Our experiments revealed that COL9A3 combined with USP3 by its M3 region, and COL6A5 interacted with USP3 through its H2 region (Fig. xref )."

34930901

USP3 deubiquitinates COL6A5.

| 1

USP3 deubiquitinates COL6A5. 1 / 1

| 1

---

➶

reach

"These data suggest that USP3 promotes GC progression and metastasis by deubiquitinating COL9A3 and COL6A5."

34930901

UBC affects USP3

3 | 1

UBC binds USP3. 3 / 3

3 |

---

➶

biogrid

No evidence text available

17980597

---

➶

biogrid

No evidence text available

10480896

---

➶

biogrid

No evidence text available

28190767

UBC binds USP3 and DDX58. 1 / 1

| 1

---

➶

sparser

"Because it was recently shown that USP3 interacts with RIG-I to deubiquitinate K63-linked poly-ubiquitin chains on RIG-I and inhibit type I IFN pathways [ xref ], we focused on SRSF1 for further experiments."

25658361

Flag affects USP3

| 4

USP3 binds Flag. 4 / 4

| 4

---

➶

sparser

"Similarly, in HEK293 cells, we observed protein stabilization of ectopically expressed Myc-Oct4 as the concentration of Flag-USP3 increased ( xref E), but expression of the catalytic mutant Flag-USP3CS had no such stabilization effect ( xref F)."

34070420

---

➶

sparser

"We co-transfected Flag-USP3 and Myc-Oct4 into HEK293 cells and performed IP with Flag or Myc antibodies, followed by Western blot with reciprocal antibodies."

34070420

---

➶

sparser

"To determine differentially expressed proteins that may serve as potential interaction targets of USP3, total protein extracted from the treated BGC-823 cells stably expressing the Flag-USP3 fusion protein and the BGC-823 cells carrying an empty vector was analyzed using iTRAQ experiments."

31234902

---

➶

sparser

"Flag-USP3 co-precipitated Myc-Oct4 ( xref A), and similarly Myc-Oct4 co-precipitated Flag-USP3 ( xref B)."

34070420

COL6A5 affects USP3

| 2 2

COL6A5 binds USP3.

| 1 2

USP3 binds COL6A5. 3 / 3

| 1 2

---

➶

sparser

"This study suggests that targeting of the USP3-COL9A3 or USP3-COL6A5 axis could be developed as a therapeutic strategy for GC management."

34930901

---

➶

reach

"Mechanistically, we observed that USP3 interacted with and stabilised COL9A3 and COL6A5 via deubiquitination in GC."

34930901

---

➶

sparser

"Our experiments revealed that COL9A3 combined with USP3 by its M3 region, and COL6A5 interacted with USP3 through its H2 region (Fig. xref )."

34930901

COL6A5 activates USP3.

| 1

COL6A5 activates USP3. 1 / 1

| 1

---

➶

reach

"Importantly, we found that COL9A3 and COL6A5 were essential mediators of USP3-modulated oncogenic activity in vitro and in vivo."

34930901

Valproic acid affects USP3

3 |

Valproic acid increases the amount of USP3.

1 |

Valproic acid increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

24935251

Valproic acid demethylates USP3.

1 |

Valproic acid demethylates USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

29154799

Valproic acid decreases the amount of USP3.

1 |

Valproic acid decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

23179753

Polyubiquitin affects USP3

| 3

USP3 binds polyubiquitin. 1 / 1

| 1

---

➶

sparser

"Mutation analysis reveals that binding of USP3 to polyubiquitin chains on RIG-I is a prerequisite step for its cleavage of polyubiquitin chains."

24366338

USP3 binds IFIH1, K63, and polyubiquitin. 1 / 1

| 1

---

➶

sparser

"In addition to RIG-I, USP3 also binds MDA5 to remove K63-polyubiquitin chains on the N-terminal CARDs [ xref ]."

28295214

USP3 binds IFIH1 and polyubiquitin. 1 / 1

| 1

---

➶

sparser

"USP3 may bind to MDA5 via unanchored polyUb chains and then deubiquitinate it [ xref ]."

33138315

Polyubiquitin chains affects USP3

| 3

USP3 binds polyubiquitin chains. 2 / 2

| 2

---

➶

reach

"We further show that there is no interaction between USP3 and RIG-I-like receptors (RLRs) in unstimulated or uninfected cells, but upon viral infection or ligand stimulation, USP3 binds to the caspase activation recruitment domain of RLRs and then cleaves polyubiquitin chains through cooperation of its zinc-finger Ub binding domain and USP catalytic domains."

24366338

---

➶

reach

"Mutation analysis reveals that binding of USP3 to polyubiquitin chains on RIG-I is a prerequisite step for its cleavage of polyubiquitin chains."

24366338

USP3 binds CARD domain and polyubiquitin chains. 1 / 1

| 1

---

➶

reach

"[164] Upon viral infection or ligand stimulation, USP3 binds to the CARD domain of RIG-I and then removes its polyubiquitin chains through both the zinc-finger Ub binding domain and USP catalytic domains."

33511009

Cyclosporin A affects USP3

3 |

Cyclosporin A increases the amount of USP3. 3 / 3

3 |

---

➶

ctd

No evidence text available

25562108

---

➶

ctd

No evidence text available

25596134

---

➶

ctd

No evidence text available

20106945

Bisphenol A affects USP3

3 |

Bisphenol A increases the amount of USP3.

2 |

Bisphenol A increases the amount of USP3. 2 / 2

2 |

---

➶

ctd

No evidence text available

30816183

---

➶

ctd

No evidence text available

25181051

Bisphenol A decreases the amount of USP3.

1 |

Bisphenol A decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

26063408

USP3 affects polyubiquitin

| 3

USP3 binds polyubiquitin. 1 / 1

| 1

---

➶

sparser

"Mutation analysis reveals that binding of USP3 to polyubiquitin chains on RIG-I is a prerequisite step for its cleavage of polyubiquitin chains."

24366338

USP3 binds IFIH1, K63, and polyubiquitin. 1 / 1

| 1

---

➶

sparser

"In addition to RIG-I, USP3 also binds MDA5 to remove K63-polyubiquitin chains on the N-terminal CARDs [ xref ]."

28295214

USP3 binds IFIH1 and polyubiquitin. 1 / 1

| 1

---

➶

sparser

"USP3 may bind to MDA5 via unanchored polyUb chains and then deubiquitinate it [ xref ]."

33138315

USP3 affects polyubiquitin chains

| 3

USP3 binds polyubiquitin chains. 2 / 2

| 2

---

➶

reach

"We further show that there is no interaction between USP3 and RIG-I-like receptors (RLRs) in unstimulated or uninfected cells, but upon viral infection or ligand stimulation, USP3 binds to the caspase activation recruitment domain of RLRs and then cleaves polyubiquitin chains through cooperation of its zinc-finger Ub binding domain and USP catalytic domains."

24366338

---

➶

reach

"Mutation analysis reveals that binding of USP3 to polyubiquitin chains on RIG-I is a prerequisite step for its cleavage of polyubiquitin chains."

24366338

USP3 binds CARD domain and polyubiquitin chains. 1 / 1

| 1

---

➶

reach

"[164] Upon viral infection or ligand stimulation, USP3 binds to the CARD domain of RIG-I and then removes its polyubiquitin chains through both the zinc-finger Ub binding domain and USP catalytic domains."

33511009

USP3 affects gammaH2AX

| 3

USP3 deubiquitinates gammaH2AX.

| 2

Modified USP3 leads to the deubiquitination of gammaH2AX. 2 / 2

| 2

---

➶

reach

"Here, we showed that ectopic expression of USP3 led to the deubiquitination of both H2A and gammaH2AX in response to UV induced DNA damage."

24196443

---

➶

reach

"In addition, while USP3 depletion resulted in delayed disappearance of ubiquitylaed H2A and FK2 staining (poly-ubiquitylation marker) after IR, over-expression of USP3 suppressed ubiquitylation of gam[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

28764946

USP3 activates gammaH2AX.

| 1

USP3 activates gammaH2AX. 1 / 1

| 1

---

➶

reach

"In addition, while USP3 depletion resulted in delayed disappearance of ubiquitylaed H2A and FK2 staining (poly-ubiquitylation marker) after IR, over-expression of USP3 suppressed ubiquitylation of gam[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

28764946

USP3 affects epithelial to mesenchymal transition

| 3

USP3 activates epithelial to mesenchymal transition. 3 / 3

| 3

---

➶

reach

"27 , 28 Recent studies have proven that USP3 promotes GBM progress by regulating the EMT process."

32918360

---

➶

reach

"Moreover, TGF-beta1 induced USP3 expression, and USP3 knockdown inhibited TGF-beta1-induced EMT."

31234902

---

➶

reach

"Previous studies have shown that USP3 raised GBM progression by regulating EMT."

32918360

USP3 affects apoptotic process

| 3

USP3 inhibits apoptotic process. 3 / 3

| 3

---

➶

reach

"USP3 knockdown induced chondrocytes apoptosis and activated NF-kappaB pathway."

31056254

---

➶

reach

"USP3 overexpression inhibited IL-1beta induced chondrocytes apoptosis and nuclear factor kappaB (NF-kappaB) activation."

31056254

---

➶

reach

"Despite it has been demonstrated that ubiquitin-specific protease 3 (USP3) inhibits chondrocyte apoptosis induced by interleukin (IL)-1β, the role of USP3/SIRT3/FOXO3 in the senescence of chondrocytes in OA is unclear."

34847835

USP3 affects UIMC1

1 | 2

USP3 inhibits UIMC1.

| 2

USP3 inhibits UIMC1. 2 / 2

| 2

---

➶

reach

"Overexpression of USP3, while having no effect on retention of RNF8 at DSBs, abolished the IR induced focus formation of RNF168, RAP80, and 53BP1, indicating that it de-ubiquitylates RNF8 targeted substrates."

23847653

---

➶

reach

"53BP1 and RAP80 foci are also negatively regulated by USP3 catalytic activity [18,110,132,135,136]."

32708614

USP3 deubiquitinates UIMC1.

1 |

USP3 deubiquitinates UIMC1. 1 / 1

1 |

---

➶

ubibrowser

"K63-linked ubiquitin accumulates on Rap80 at the DSB foci with the concerted effect of RNF8, RNF168, and Ubc13, which are clipped off with the assistance of USP3 and BRCC36 to maintain the G2/M checkpoint."

25121098

USP3 affects TRAF6

| 2 1

USP3 binds TRAF6.

| 1 1

TRAF6 binds USP3. 2 / 2

| 1 1

---

➶

sparser

"USP3 interacts with TRAF6 (tumor necrosis factor-receptor-associated factor 6), which is an essential adaptor protein for the NF-κB (nuclear factor κB) signaling pathway and plays important roles in inflammation and immune response."

31056254

---

➶

reach

"USP3 interacts with TRAF6 (tumor necrosis factor-receptor-associated factor 6), which is an essential adaptor protein for the NF-kappaB (nuclear factor kappaB) signaling pathway and plays important roles in inflammation and immune response."

31056254

USP3 deubiquitinates TRAF6.

| 1

USP3 leads to the deubiquitination of TRAF6. 1 / 1

| 1

---

➶

reach

"Moreover, USP3 can reverse the ubiquitination of TRAF6 (tumor necrosis factor-receptor-associated factor 6), which intermediates the signals from inflammatory cytokines to NF-κB activation, thus blocking IL-1β-induced chondrocyte apoptosis [27]."

34216299

USP3 affects TBK1

| 1 2

USP3 inhibits TBK1. 3 / 3

| 1 2

---

➶

sparser

"USP3 did not inhibit MAVS, STING, TBK1, IRF3 and TIRF, as demonstrated by ISRE-luc activity induction test [25]."

31208841

---

➶

sparser

"USP3 did not inhibit MAVS, STING, TBK1, IRF3 and TIRF, as demonstrated by ISRE-luc activity induction test [ xref ]."

31208841

---

➶

eidos

"USP3 did not inhibit MAVS , STING , TBK1 , IRF3 and TIRF , as demonstrated by ISRE-luc activity induction test [ 25 ] ."

31208841

USP3 affects REST

| 2 1

USP3 binds REST.

| 1 1

USP3 binds REST. 2 / 2

| 1 1

---

➶

sparser

"The interaction between USP3 and REST was confirmed by immunoprecipitation and Duolink in situ proximity assays."

37170124

---

➶

reach

"The interaction between USP3 and REST was confirmed by immunoprecipitation and Duolink in situ proximity assays."

37170124

USP3 activates REST.

| 1

USP3 activates REST. 1 / 1

| 1

---

➶

reach

"We identified a deubiquitinase USP3 that interacts with, stabilizes, and increases the half-life of REST protein by counteracting its ubiquitination in neuroblastoma."

37170124

USP3 affects PKLR

1 | 2

USP3 binds PKLR. 3 / 3

1 | 2

---

➶

sparser

"These findings imply that USP3 is an essential positive regulator in GBC progression, and USP3-PKLR plays a vital role in the progression and metabolism of GBC."

36180571

---

➶

biogrid

No evidence text available

19615732

---

➶

sparser

"These findings imply that USP3 is an essential positive regulator in GBC progression, and USP3-PKLR plays a vital role in the progression and metabolism of GBC."

36792297

USP3 affects Neoplasms

| 1 1

USP3 activates Neoplasms. 2 / 3

| 1 1

---

➶

eidos

"Our previous study revealed that USP3 promotes tumour progression and is highly expressed in gastric cancer ( GC ) ."

34930901

---

➶

reach

"Additionally, overexpressed USP3 significantly promoted cell proliferation in vitro and tumor growth in vivo, while the silencing of USP3 inhibited proliferation and tumor growth."

36792297

USP3 affects H2AX

1 | 1 1

USP3 deubiquitinates H2AX.

1 | 1

USP3 deubiquitinates H2AX. 2 / 2

1 | 1

---

➶

reach

"USP3, USP51 and USP16 de-ubiquitinate H2A/H2AX with varying degrees of specificity [47–49]."

31769469

---

➶

ubibrowser

"Review"

28031783

USP3 binds H2AX.

| 1

USP3 binds H2AX. 1 / 1

| 1

---

➶

sparser

"These effects may be related to a functional interaction between USP3 and the isoform g-H2AX which accumulates at doubled stranded breaks."

21513801

USP3 affects COL9A3

| 2 1

USP3 binds COL9A3.

| 1 1

USP3 binds COL9A3. 2 / 2

| 1 1

---

➶

sparser

"This study suggests that targeting of the USP3-COL9A3 or USP3-COL6A5 axis could be developed as a therapeutic strategy for GC management."

34930901

---

➶

reach

"Mechanistically, we observed that USP3 interacted with and stabilised COL9A3 and COL6A5 via deubiquitination in GC."

34930901

USP3 deubiquitinates COL9A3.

| 1

USP3 deubiquitinates COL9A3. 1 / 1

| 1

---

➶

reach

"These data suggest that USP3 promotes GC progression and metastasis by deubiquitinating COL9A3 and COL6A5."

34930901

USP3 affects CHEK1

1 | 2

USP3 inhibits CHEK1.

| 1

USP3 inhibits CHEK1. 1 / 1

| 1

---

➶

reach

"Wild-type USP3, but not the catalytically defective or nuclear localization sequence deficient mutants, reduced CHK1 K63 linked ubiquitination."

29735693

USP3 deubiquitinates CHEK1.

1 |

USP3 deubiquitinates CHEK1. 1 / 1

1 |

---

➶

ubibrowser

"Herein, we show that the K63-linked ubiquitin chain at CHK1's K132 residue has an inhibitory effect on the kinase activity. Furthermore, we demonstrate that this modification can be removed by ubiquitin-specific protease 3 (USP3), a deubiquitinating enzyme that targets K63-linked ubiquitin chains."

29735693

USP3 activates CHEK1.

| 1

USP3 activates CHEK1. 1 / 1

| 1

---

➶

reach

"Paradoxically, by removing the bulky ubiquitin chain at the active site, USP3 also increased the accessibility of CHK1 to its substrates."

29735693

SNAI1 affects USP3

| 2 1

SNAI1 binds USP3.

| 1 1

SNAI1 binds USP3. 2 / 2

| 1 1

---

➶

sparser

"USP3 interacts directly with Snail and stabilizes Snail via deubiquitination."

31266817

---

➶

reach

"USP3 interacts directly with Snail and stabilizes Snail via deubiquitination."

31266817

SNAI1 inhibits USP3.

| 1

SNAI1 inhibits USP3. 1 / 1

| 1

---

➶

reach

"Ectopic expression of Snail could largely rescue the inhibitory effects of USP3 depletion on migration, invasion and tumor growth of GBM cells."

31266817

SMAD4 affects USP3

| 3

SMAD4 binds USP3.

| 2

USP3 binds SMAD4. 2 / 2

| 2

---

➶

reach

"We utilized luciferase reporter constructs of PGL3-USP3 3 ' UTR and PGL3-SMAD4 3 ' UTR to evaluate whether the interaction between USP3 and SMAD4 was 3 ' UTR dependent."

28655924

---

➶

reach

"This reversal was partially due to the ceRNA interaction between USP3 and SMAD4."

28655924

SMAD4 increases the amount of USP3.

| 1

SMAD4 increases the amount of USP3. 1 / 1

| 1

---

➶

reach

"Similarly, knockdown of SMAD4 attenuated USP3 expression at both the protein and the mRNA levels."

28655924

PKLR affects USP3

1 | 2

USP3 binds PKLR. 3 / 3

1 | 2

---

➶

sparser

"These findings imply that USP3 is an essential positive regulator in GBC progression, and USP3-PKLR plays a vital role in the progression and metabolism of GBC."

36180571

---

➶

biogrid

No evidence text available

19615732

---

➶

sparser

"These findings imply that USP3 is an essential positive regulator in GBC progression, and USP3-PKLR plays a vital role in the progression and metabolism of GBC."

36792297

PDS5B affects USP3

| 2 1

PDS5B decreases the amount of USP3.

| 1

PDS5B decreases the amount of USP3. 1 / 1

| 1

---

➶

reach

"Western blot analysis showed that down‐regulation of lncRNA HOXA‐AS3 inhibits USP3 expression and EMT in vivo (Figure 6E).4 DISCUSSION."

32918360

PDS5B binds USP3.

| 1

USP3 binds PDS5B and hoxA. 1 / 1

| 1

---

➶

sparser

"In conclusion, we found an associated lncRNA network that regulates the tumour cell development network, consisting of lncRNA HOXA‐AS3, miR‐455‐5p and USP3."

32918360

PDS5B activates USP3.

| 1

PDS5B activates USP3. 1 / 1

| 1

---

➶

reach

"These results indicate that lncRNA HOXA‐AS3 can promote glioma progression by regulating the miR‐455‐5p/ USP3 axis.Epithelial‐mesenchymal transition is a process in which epithelial cells are transformed into mesenchymal cells and acquire motile and invasive characteristics."

32918360

KLF5 affects USP3

| 1 2

USP3 binds KLF5. 2 / 2

| 1 1

---

➶

sparser

"We demonstrated that USP3 interacts with KLF5 and stabilizes KLF5 via deubiquitination."

31624151

---

➶

reach

"We demonstrated that USP3 interacts with KLF5 and stabilizes KLF5 via deubiquitination."

31624151

USP3 binds KLF5 and BAP1. 1 / 1

| 1

---

➶

sparser

"Both BAP1 and USP3 bind to and stabilize KLF5 via deubiquitination [ xref , xref ], indicating the possible regulatory role of DUBs in cancer proliferation."

32268558

IFIH1 affects DDX58

| 3

USP3 binds IFIH1 and DDX58. 3 / 3

| 3

---

➶

sparser

"The interaction of USP3 with RIG-I and MDA5 is strictly based upon ligand stimulation."

33138315

---

➶

sparser

"Also co-IP demonstrated the interaction between USP3 and stimulated RIG-I or MDA5, but not the unstimulated ones, supporting that ligand stimulation is required for USP3 to interact with RIG-I or MDA5 [ xref ]."

31208841

---

➶

sparser

"Also co-IP demonstrated the interaction between USP3 and stimulated RIG-I or MDA5, but not the unstimulated ones, supporting that ligand stimulation is required for USP3 to interact with RIG-I or MDA5 [25]."

31208841

HNF1A affects USP3

3 |

HNF1A decreases the amount of USP3. 3 / 3

3 |

---

➶

biopax:msigdb

No evidence text available

---

➶

biopax:msigdb

No evidence text available

---

➶

biopax:msigdb

No evidence text available

DDX58 affects IFIH1, and USP3

| 3

USP3 binds IFIH1 and DDX58. 3 / 3

| 3

---

➶

sparser

"The interaction of USP3 with RIG-I and MDA5 is strictly based upon ligand stimulation."

33138315

---

➶

sparser

"Also co-IP demonstrated the interaction between USP3 and stimulated RIG-I or MDA5, but not the unstimulated ones, supporting that ligand stimulation is required for USP3 to interact with RIG-I or MDA5 [ xref ]."

31208841

---

➶

sparser

"Also co-IP demonstrated the interaction between USP3 and stimulated RIG-I or MDA5, but not the unstimulated ones, supporting that ligand stimulation is required for USP3 to interact with RIG-I or MDA5 [25]."

31208841

COL9A3 affects USP3

| 2 1

COL9A3 binds USP3.

| 1 1

USP3 binds COL9A3. 2 / 2

| 1 1

---

➶

sparser

"This study suggests that targeting of the USP3-COL9A3 or USP3-COL6A5 axis could be developed as a therapeutic strategy for GC management."

34930901

---

➶

reach

"Mechanistically, we observed that USP3 interacted with and stabilised COL9A3 and COL6A5 via deubiquitination in GC."

34930901

COL9A3 activates USP3.

| 1

COL9A3 activates USP3. 1 / 1

| 1

---

➶

reach

"Importantly, we found that COL9A3 and COL6A5 were essential mediators of USP3-modulated oncogenic activity in vitro and in vivo."

34930901

Polyubiquitin affects IFIH1

| 2

USP3 binds IFIH1, K63, and polyubiquitin. 1 / 1

| 1

---

➶

sparser

"In addition to RIG-I, USP3 also binds MDA5 to remove K63-polyubiquitin chains on the N-terminal CARDs [ xref ]."

28295214

USP3 binds IFIH1 and polyubiquitin. 1 / 1

| 1

---

➶

sparser

"USP3 may bind to MDA5 via unanchored polyUb chains and then deubiquitinate it [ xref ]."

33138315

Phorbol 13-acetate 12-myristate affects USP3

1 | 1

Phorbol 13-acetate 12-myristate increases the amount of USP3.

| 1

Phorbol 13-acetate 12-myristate increases the amount of USP3. 1 / 1

| 1

---

➶

reach

"Levels of USP3, one of the deubiquitinating enzymes (DUBs), increased by TPA treatment, resulting in the reduction of H2AK119ub levels."

31700696

Phorbol 13-acetate 12-myristate decreases the amount of USP3.

1 |

Phorbol 13-acetate 12-myristate decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

25613284

Phenethyl isothiocyanate affects USP3

| 1 1

Phenethyl isothiocyanate inhibits USP3. 2 / 2

| 1 1

---

➶

reach

"The inhibition of USP3 by PEITC in living cells was confirmed by observing the molecular shift upon probe labeling by immunoblotting."

28881649

---

➶

sparser

"The inhibition of USP3 by PEITC in living cells was confirmed by observing the molecular shift upon probe labeling by immunoblotting (Figure xref )."

28881649

LncRNA HOXA-AS3 affects USP3

| 2

LncRNA HOXA-AS3 activates USP3. 2 / 2

| 2

---

➶

eidos

"Western blot analysis showed that down-regulation of lncRNA HOXA-AS3 inhibits USP3 expression and EMT in vivo ( Figure 6E ) ."

32918360

---

➶

eidos

"We also found that lncRNA HOXA-AS3 could act as a miRNA sponge to reduce miR-455-5p concentration , up-regulate USP3 expression and drive the epithelial-mesenchymal transition ( EMT ) process of GBM cells ."

32918360

Hsa-miR-26b-5p affects USP3

2 |

Hsa-miR-26b-5p decreases the amount of USP3. 2 / 2

2 |

---

➶

biopax:mirtarbase

No evidence text available

22473208

---

➶

biopax:mirtarbase

No evidence text available

19088304

CarD affects USP3

| 2

USP3 binds carD. 1 / 1

| 1

---

➶

sparser

"Upon viral infection, USP3 directly interacts with the CARD of RIG-I and cleaves the K63-linked polyUb chains, but not the K48-linked polyUb chain, thus converting RIG-I to its inactive form [ xref ]."

33138315

USP3 binds IFIH1 and carD. 1 / 1

| 1

---

➶

sparser

"While the deubiquitinase USP3 inhibits MDA5 as well as RIG-I, it is thought that this may be due to USP3 directly binding the MDA5 CARD domain to prevent RNA filamentation (284)."

31379819

Aflatoxin B1 affects USP3

2 |

Aflatoxin B1 increases the amount of USP3.

1 |

Aflatoxin B1 increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

19770486

Aflatoxin B1 demethylates USP3.

1 |

Aflatoxin B1 demethylates USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

27153756

USP3 affects immune response

| 2

USP3 inhibits immune response. 2 / 2

| 2

---

➶

reach

"USP2b, USP3, USP18, USP25, UL36USP and HAUSP play a role of antivirus; while USP4, USP13, USP15 and USP17 negatively regulate antiviral immune response."

26713535

---

➶

reach

"Our findings identify a previously unrecognized role of USP3 in RIG-I activation and provide insights into the mechanisms by which USP3 inhibits RIG-I signaling and antiviral immunity."

24366338

USP3 affects ecdysone

| 2

USP3 activates ecdysone. 2 / 2

| 2

---

➶

reach

"Activation of BrC-Z1 as well as EcR, itself an ecdysone response gene, can be mediated by both the USP3 and USP4 mutant proteins."

11274407

---

➶

reach

"USP3 and USP4 also activate an ecdysone responsive element, hsp27EcRE, in cultured cells."

11274407

USP3 affects carD

| 2

USP3 binds carD. 1 / 1

| 1

---

➶

sparser

"Upon viral infection, USP3 directly interacts with the CARD of RIG-I and cleaves the K63-linked polyUb chains, but not the K48-linked polyUb chain, thus converting RIG-I to its inactive form [ xref ]."

33138315

USP3 binds IFIH1 and carD. 1 / 1

| 1

---

➶

sparser

"While the deubiquitinase USP3 inhibits MDA5 as well as RIG-I, it is thought that this may be due to USP3 directly binding the MDA5 CARD domain to prevent RNA filamentation (284)."

31379819

USP3 affects activity

| 2

USP3 inhibits activity. 2 / 2

| 2

---

➶

sparser

"Ectopic expression of USP3 inhibited IFN-β promoter activity in cells treated with high molecular weight (HMW) poly(I:C), whereas gene silencing of USP3 enhanced IFN-β protein expression under similar treatment."

28295214

---

➶

sparser

"Ectopic expression of USP3 inhibited IFN-β promoter activity in cells treated with low molecular weight (LMW) poly(I:C), and in cells infected with VSV."

28295214

USP3 affects Ubiquitination

| 2

USP3 inhibits Ubiquitination. 2 / 2

| 2

---

➶

eidos

"Moreover , the loss of USP3 promoted the ubiquitination and weakened the stability of PKLR ."

36180571

---

➶

eidos

"USP3 , USP21 and CYLD inhibit RIG-I K63-linked ubiquitination and activation ."

28744851

USP3 affects Protease

| 2

USP3 inhibits Protease.

| 1

USP3 inhibits Protease. 1 / 1

| 1

---

➶

reach

"Ub specific protease (USP) 3 : The Ub modifications on K119/120 of histone H2A and on K13/15 of histone H2B generated by BMI1 and RNF2 and RNF20-RNF40 are attenuated by USP3, an H2A- and H2B specific [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

28587922

USP3 activates Protease.

| 1

USP3 activates Protease. 1 / 1

| 1

---

➶

reach

"USP3 produces a protease that breaks down and modulates many intracellular proteins and eliminates damaged and misfolded proteins and regulates cellular processes as apoptosis."

31552271

USP3 affects Neoplasm Invasiveness

| 2

USP3 activates Neoplasm Invasiveness. 2 / 2

| 2

---

➶

reach

"Silencing of USP3 attenuates the migration and invasion abilities of GBM cells in vitro and tumor growth in an orthotopic xenograft mouse model."

31266817

---

➶

reach

"Cellular experiments confirmed that high expression of USP3 and Aurora A in ESCC cells promoted malignant cell proliferation and invasion."

34758762

USP3 affects MYCN

| 1 1

USP3 deubiquitinates MYCN.

| 1

USP3 leads to the deubiquitination of MYCN. 1 / 1

| 1

---

➶

reach

"The known role of ALYREF as a regulator of DNA binding guided further analyses that uncovered ALYREF-MYCN interaction in a nuclear coactivator complex which stimulates transcription of the ‘ubiquitin specific peptidase 3′ (USP3), consequently reducing MYCN ubiquitination and degradation."

| PMC

USP3 binds MYCN.

| 1

USP3 binds MYCN. 1 / 1

| 1

---

➶

sparser

"USP3 interacts directly with its protein targets, which prompted us to examine whether MYCN also bound USP3 directly."

33767157

USP3 affects ISLR

2 |

USP3 binds ISLR. 2 / 2

2 |

---

➶

biogrid

No evidence text available

28514442

---

➶

biogrid

No evidence text available

26186194

USP3 affects H2B

| 2

USP3 inhibits H2B.

| 1

USP3 inhibits H2B. 1 / 1

| 1

---

➶

reach

"Overexpression of Usp3 in HeLa cells causes the reduction in both uH2A and uH2B without altering the bulk pool of ubiquitinated proteins."

19489724

USP3 decreases the amount of H2B.

| 1

Modified USP3 decreases the amount of H2B. 1 / 1

| 1

---

➶

reach

"Overexpression of USP3 in HeLa cells reduced levels of both Ub-H2A and Ub-H2B, whereas knockdown of USP3 enhanced the ubiquitination of both histones."

18226187

USP3 affects H2AC20

1 | 1

USP3 deubiquitinates H2AC20.

1 |

USP3 deubiquitinates H2AC20. 1 / 1

1 |

---

➶

ubibrowser

"USP3 counteracts RNF168 via deubiquitinating H2A and gammaH2AX at lysine 13 and 15."

24196443

USP3 binds H2AC20.

| 1

USP16 binds USP3 and H2AC20. 1 / 1

| 1

---

➶

sparser

"Both USP3 and USP16 are also associated with H2A deubiquitination following DNA damage processing [ xref , xref ]."

20974139

USP3 affects Glioblastoma

| 2

USP3 activates Glioblastoma. 2 / 2

| 2

---

➶

eidos

"Previous studies have shown that USP3 raised GBM progression by regulating EMT ."

32918360

---

➶

eidos

"The dual-luciferase reporter assay also revealed that lncRNA HOXA-AS3 acts as a mir-455-5p sponge by up-regulating USP3 expression to promote GBM progression ."

32918360

USP3 affects Esophageal Squamous Cell Carcinoma

| 2

USP3 activates Esophageal Squamous Cell Carcinoma. 2 / 2

| 2

---

➶

reach

"Here, we report that ubiquitin specific peptidase 3 (USP3) promotes proliferation and metastasis of esophageal squamous cell carcinoma (ESCC) cells by mediating deubiquitination of Aurora A. Analysis of human clinical samples indicated that USP3 and Aurora A are highly expressed in ESCC."

34758762

---

➶

reach

"Protein deubiquitylase USP3 stabilizes Aurora A to promote proliferation and metastasis of esophageal squamous cell carcinoma."

34758762

USP3 affects DNA damage response, detection of DNA damage

| 2

USP3 activates DNA damage response, detection of DNA damage. 2 / 2

| 2

---

➶

reach

"Overall, despite the non-specificity towards H2A ubiquitination [74], current data support the notion that USP3, at least in part, counteracts the RNF168-mediated pathway in regulating the DDR."

33797206

---

➶

reach

"Isolation of USP3 interacting partners and potential additional substrates, as well as addressing if and how USP3 is regulated, is needed to gain a better understanding of the molecular mechanism of USP3 mediated DDR."

18430235

USP3 affects ALYREF

| 1 1

USP3 inhibits ALYREF.

| 1

USP3 inhibits ALYREF. 1 / 1

| 1

---

➶

reach

"Conversely, the removal of Lys63-linked ubiquitylation by the cellular deubiquitylating enzymes (DUBs) ubiquitin C-terminal hydrolase 3 (USP3), USP21 and CYLD, represses RIG-I signalling (reviewed in Ref."

27174148

USP3 binds ALYREF.

| 1

USP3 binds ALYREF. 1 / 1

| 1

---

➶

sparser

"Here, we have described a mechanism for MYCN degradation in MYCN-dependent neuroblastoma, regulated by the ALYREF-USP3 signal."

33767157

TRAF6 affects USP3

| 1 1

TRAF6 binds USP3. 2 / 2

| 1 1

---

➶

sparser

"USP3 interacts with TRAF6 (tumor necrosis factor-receptor-associated factor 6), which is an essential adaptor protein for the NF-κB (nuclear factor κB) signaling pathway and plays important roles in inflammation and immune response."

31056254

---

➶

reach

"USP3 interacts with TRAF6 (tumor necrosis factor-receptor-associated factor 6), which is an essential adaptor protein for the NF-kappaB (nuclear factor kappaB) signaling pathway and plays important roles in inflammation and immune response."

31056254

TGFB1 affects USP3

| 2

TGFB1 increases the amount of USP3. 2 / 2

| 2

---

➶

sparser

"Moreover, TGF-β1 induced USP3 expression, and USP3 knockdown inhibited TGF-β1-induced EMT."

31234902

---

➶

sparser

"Blocking TGF-β1 with a neutralizing antibody significantly suppressed endogenous USP3 expression and TGF-β1-induced USP3 expression in BGC-823 cells (Additional file xref : Figure S2C)."

31234902

REST affects USP3

| 1 1

USP3 binds REST. 2 / 2

| 1 1

---

➶

sparser

"The interaction between USP3 and REST was confirmed by immunoprecipitation and Duolink in situ proximity assays."

37170124

---

➶

reach

"The interaction between USP3 and REST was confirmed by immunoprecipitation and Duolink in situ proximity assays."

37170124

MicroRNA-146-5p affects USP3

| 2

MicroRNA-146-5p activates USP3. 2 / 2

| 2

---

➶

reach

"MicroRNA-146-5p Promotes Pulmonary Artery Endothelial Cell Proliferation under Hypoxic Conditions through Regulating USP3."

34917199

---

➶

reach

"MicroRNA-146-5p was able to enhance the proliferation ability of HPAEC cells under hypoxic conditions through targeting USP3, suggesting the microRNA-146-5p/USP3 axis may act as a target for PAH treatment."

34917199

LEF1 affects USP3

2 |

LEF1 decreases the amount of USP3. 2 / 2

2 |

---

➶

biopax:msigdb

No evidence text available

---

➶

biopax:msigdb

No evidence text available

ISLR affects USP3

2 |

USP3 binds ISLR. 2 / 2

2 |

---

➶

biogrid

No evidence text available

28514442

---

➶

biogrid

No evidence text available

26186194

IFIH1 affects polyubiquitin

| 2

USP3 binds IFIH1, K63, and polyubiquitin. 1 / 1

| 1

---

➶

sparser

"In addition to RIG-I, USP3 also binds MDA5 to remove K63-polyubiquitin chains on the N-terminal CARDs [ xref ]."

28295214

USP3 binds IFIH1 and polyubiquitin. 1 / 1

| 1

---

➶

sparser

"USP3 may bind to MDA5 via unanchored polyUb chains and then deubiquitinate it [ xref ]."

33138315

FOXO4 affects USP3

2 |

FOXO4 decreases the amount of USP3. 2 / 2

2 |

---

➶

biopax:msigdb

No evidence text available

---

➶

biopax:msigdb

No evidence text available

ETS2 affects USP3

2 |

ETS2 decreases the amount of USP3. 2 / 2

2 |

---

➶

biopax:msigdb

No evidence text available

---

➶

biopax:msigdb

No evidence text available

2,3,7,8-tetrachlorodibenzodioxine affects USP3

2 |

2,3,7,8-tetrachlorodibenzodioxine increases the amount of USP3.

1 |

2,3,7,8-tetrachlorodibenzodioxine increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

22903824

2,3,7,8-tetrachlorodibenzodioxine decreases the amount of USP3.

1 |

2,3,7,8-tetrachlorodibenzodioxine decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

21215274

Tris(1,3-dichloro-2-propyl)phosphate affects USP3

1 |

Tris(1,3-dichloro-2-propyl)phosphate increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

26179874

Trichostatin A affects USP3

1 |

Trichostatin A increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

24935251

Topotecan affects USP3

1 |

Topotecan decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

25729387

Titanium dioxide affects USP3

1 |

Titanium dioxide increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

23557971

Thioacetamide affects USP3

1 |

Thioacetamide increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

23411599

Selenium atom affects USP3

1 |

Selenium atom decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

19244175

Rosiglitazone affects USP3

1 |

Rosiglitazone increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

25596134

Polyubiquitin affects K63

| 1

USP3 binds IFIH1, K63, and polyubiquitin. 1 / 1

| 1

---

➶

sparser

"In addition to RIG-I, USP3 also binds MDA5 to remove K63-polyubiquitin chains on the N-terminal CARDs [ xref ]."

28295214

Polyubiquitin chains affects CARD domain

| 1

USP3 binds CARD domain and polyubiquitin chains. 1 / 1

| 1

---

➶

reach

"[164] Upon viral infection or ligand stimulation, USP3 binds to the CARD domain of RIG-I and then removes its polyubiquitin chains through both the zinc-finger Ub binding domain and USP catalytic domains."

33511009

Peptidase activity affects USP3

| 1

Peptidase activity binds USP3. 1 / 1

| 1

---

➶

sparser

"The two proteins form a nuclear coactivator complex which stimulates transcription of the ubiquitin specific peptidase 3, USP3."

33767157

Pentachlorophenol affects USP3

1 |

Pentachlorophenol decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

23892564

Oxaliplatin affects USP3

1 |

Oxaliplatin decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

25729387

Nefazodone affects USP3

1 |

Nefazodone increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

24136188

Mono(2-ethylhexyl) phthalate affects USP3

1 |

Mono(2-ethylhexyl) phthalate decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

31059758

MicroRNA-146-5p affects USP3

| 1

USP3 binds microRNA-146-5p. 1 / 1

| 1

---

➶

reach

"In addition, the interaction between microRNA-146-5p and USP3 was examined by luciferase reporting assay."

34917199

MiR-33a affects USP3

| 1

MiR-33a decreases the amount of USP3. 1 / 1

| 1

---

➶

reach

"For supportive host factors, miR-1-3p, miR-17-3p, miR-193b, miR-203, miR-221, miR-26a, miR-30, miR-324-5p, miR-33a, and miR-548an respectively inhibit the expression of ATP6V1A, GALNT3, β-catenin, DR1, GALNT3, USP3, NEDD4, CUEDC2, ARCN1, and NS1ABP to inhibit IAV replication ultimately, but miR-34c promotes PLK4 expression to promote IAV replication."

35476817

MiR-324-5p affects USP3

| 1

MiR-324-5p decreases the amount of USP3. 1 / 1

| 1

---

➶

reach

"For supportive host factors, miR-1-3p, miR-17-3p, miR-193b, miR-203, miR-221, miR-26a, miR-30, miR-324-5p, miR-33a, and miR-548an respectively inhibit the expression of ATP6V1A, GALNT3, β-catenin, DR1, GALNT3, USP3, NEDD4, CUEDC2, ARCN1, and NS1ABP to inhibit IAV replication ultimately, but miR-34c promotes PLK4 expression to promote IAV replication."

35476817

MiR-30 affects USP3

| 1

MiR-30 decreases the amount of USP3. 1 / 1

| 1

---

➶

reach

"For supportive host factors, miR-1-3p, miR-17-3p, miR-193b, miR-203, miR-221, miR-26a, miR-30, miR-324-5p, miR-33a, and miR-548an respectively inhibit the expression of ATP6V1A, GALNT3, β-catenin, DR1, GALNT3, USP3, NEDD4, CUEDC2, ARCN1, and NS1ABP to inhibit IAV replication ultimately, but miR-34c promotes PLK4 expression to promote IAV replication."

35476817

MiR-26a affects USP3

| 1

MiR-26a decreases the amount of USP3. 1 / 1

| 1

---

➶

reach

"For supportive host factors, miR-1-3p, miR-17-3p, miR-193b, miR-203, miR-221, miR-26a, miR-30, miR-324-5p, miR-33a, and miR-548an respectively inhibit the expression of ATP6V1A, GALNT3, β-catenin, DR1, GALNT3, USP3, NEDD4, CUEDC2, ARCN1, and NS1ABP to inhibit IAV replication ultimately, but miR-34c promotes PLK4 expression to promote IAV replication."

35476817

MiR-221 affects USP3

| 1

MiR-221 decreases the amount of USP3. 1 / 1

| 1

---

➶

reach

"For supportive host factors, miR-1-3p, miR-17-3p, miR-193b, miR-203, miR-221, miR-26a, miR-30, miR-324-5p, miR-33a, and miR-548an respectively inhibit the expression of ATP6V1A, GALNT3, β-catenin, DR1, GALNT3, USP3, NEDD4, CUEDC2, ARCN1, and NS1ABP to inhibit IAV replication ultimately, but miR-34c promotes PLK4 expression to promote IAV replication."

35476817

MiR-203 affects USP3

| 1

MiR-203 decreases the amount of USP3. 1 / 1

| 1

---

➶

reach

"For supportive host factors, miR-1-3p, miR-17-3p, miR-193b, miR-203, miR-221, miR-26a, miR-30, miR-324-5p, miR-33a, and miR-548an respectively inhibit the expression of ATP6V1A, GALNT3, β-catenin, DR1, GALNT3, USP3, NEDD4, CUEDC2, ARCN1, and NS1ABP to inhibit IAV replication ultimately, but miR-34c promotes PLK4 expression to promote IAV replication."

35476817

MiR-193b affects USP3

| 1

MiR-193b decreases the amount of USP3. 1 / 1

| 1

---

➶

reach

"For supportive host factors, miR-1-3p, miR-17-3p, miR-193b, miR-203, miR-221, miR-26a, miR-30, miR-324-5p, miR-33a, and miR-548an respectively inhibit the expression of ATP6V1A, GALNT3, β-catenin, DR1, GALNT3, USP3, NEDD4, CUEDC2, ARCN1, and NS1ABP to inhibit IAV replication ultimately, but miR-34c promotes PLK4 expression to promote IAV replication."

35476817

MiR-17-3p affects USP3

| 1

MiR-17-3p decreases the amount of USP3. 1 / 1

| 1

---

➶

reach

"For supportive host factors, miR-1-3p, miR-17-3p, miR-193b, miR-203, miR-221, miR-26a, miR-30, miR-324-5p, miR-33a, and miR-548an respectively inhibit the expression of ATP6V1A, GALNT3, β-catenin, DR1, GALNT3, USP3, NEDD4, CUEDC2, ARCN1, and NS1ABP to inhibit IAV replication ultimately, but miR-34c promotes PLK4 expression to promote IAV replication."

35476817

MiR-1-3p affects USP3

| 1

MiR-1-3p decreases the amount of USP3. 1 / 1

| 1

---

➶

reach

"For supportive host factors, miR-1-3p, miR-17-3p, miR-193b, miR-203, miR-221, miR-26a, miR-30, miR-324-5p, miR-33a, and miR-548an respectively inhibit the expression of ATP6V1A, GALNT3, β-catenin, DR1, GALNT3, USP3, NEDD4, CUEDC2, ARCN1, and NS1ABP to inhibit IAV replication ultimately, but miR-34c promotes PLK4 expression to promote IAV replication."

35476817

Methyl methanesulfonate affects USP3

1 |

Methyl methanesulfonate decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

23649840

Methotrexate affects USP3

1 |

Methotrexate decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

24449571

Metformin affects USP3

1 |

Metformin increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

25596134

Isotretinoin affects USP3

1 |

Isotretinoin decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

20436886

Ionomycin affects USP3

1 |

Ionomycin decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

25613284

Ifosfamide affects USP3

1 |

Ifosfamide increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

25596134

Hsa-miR-98-5p affects USP3

1 |

Hsa-miR-98-5p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

19088304

Hsa-miR-93-5p affects USP3

1 |

Hsa-miR-93-5p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

22473208

Hsa-miR-6734-3p affects USP3

1 |

Hsa-miR-6734-3p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

19536157

Hsa-miR-670-3p affects USP3

1 |

Hsa-miR-670-3p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

19536157

Hsa-miR-519d-3p affects USP3

1 |

Hsa-miR-519d-3p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

22473208

Hsa-miR-4279 affects USP3

1 |

Hsa-miR-4279 decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

19536157

Hsa-miR-3667-3p affects USP3

1 |

Hsa-miR-3667-3p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

19536157

Hsa-miR-337-3p affects USP3

1 |

Hsa-miR-337-3p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

19536157

Hsa-miR-26a-5p affects USP3

1 |

Hsa-miR-26a-5p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

22473208

Hsa-miR-20b-5p affects USP3

1 |

Hsa-miR-20b-5p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

22473208

Hsa-miR-20a-5p affects USP3

1 |

Hsa-miR-20a-5p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

22473208

Hsa-miR-202-5p affects USP3

1 |

Hsa-miR-202-5p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

19536157

Hsa-miR-195-5p affects USP3

1 |

Hsa-miR-195-5p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

22473208

Hsa-miR-18a-3p affects USP3

1 |

Hsa-miR-18a-3p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-17-5p affects USP3

1 |

Hsa-miR-17-5p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

22473208

Hsa-miR-16-5p affects USP3

1 |

Hsa-miR-16-5p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

22473208

Hsa-miR-15b-5p affects USP3

1 |

Hsa-miR-15b-5p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

22473208

Hsa-miR-15a-5p affects USP3

1 |

Hsa-miR-15a-5p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

22473208

Hsa-miR-10b-5p affects USP3

1 |

Hsa-miR-10b-5p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-106b-5p affects USP3

1 |

Hsa-miR-106b-5p decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:mirtarbase

No evidence text available

22473208

HoxA affects USP3

| 1

USP3 binds PDS5B and hoxA. 1 / 1

| 1

---

➶

sparser

"In conclusion, we found an associated lncRNA network that regulates the tumour cell development network, consisting of lncRNA HOXA‐AS3, miR‐455‐5p and USP3."

32918360

Hexabromocyclododecane affects USP3

1 |

Hexabromocyclododecane decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

29177809

Gentamycin affects USP3

1 |

Gentamycin increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

22061828

Formaldehyde affects USP3

1 |

Formaldehyde decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

23649840

Folic acid affects USP3

1 |

Folic acid methylates USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

20938992

Flutamide affects USP3

1 |

Flutamide increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

24136188

Fenofibrate affects USP3

1 |

Fenofibrate increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

25596134

Ethyl methanesulfonate affects USP3

1 |

Ethyl methanesulfonate decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

23649840

Dioxygen affects USP3

1 |

Dioxygen increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

25596134

Diarsenic trioxide affects USP3

1 |

Diarsenic trioxide increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

20458559

Dexamethasone affects USP3

1 |

Dexamethasone increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

14561216

Coumestrol affects USP3

1 |

Coumestrol decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

19167446

Copper(II) sulfate affects USP3

1 |

Copper(II) sulfate increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

19549813

Choline affects USP3

1 |

Choline methylates USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

20938992

Chloroacetaldehyde affects USP3

1 |

Chloroacetaldehyde increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

25596134

Carbon nanotube affects USP3

1 |

Carbon nanotube increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

25554681

CarD affects IFIH1

| 1

USP3 binds IFIH1 and carD. 1 / 1

| 1

---

➶

sparser

"While the deubiquitinase USP3 inhibits MDA5 as well as RIG-I, it is thought that this may be due to USP3 directly binding the MDA5 CARD domain to prevent RNA filamentation (284)."

31379819

Benzo[a]pyrene affects USP3

1 |

Benzo[a]pyrene increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

22228805

Benzo[a]pyrene diol epoxide I affects USP3

1 |

Benzo[a]pyrene diol epoxide I decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

19150397

Benzene affects USP3

1 |

Benzene increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

15929907

Abrine affects USP3

1 |

Abrine increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

31054353

ZFAND6 affects USP3

1 |

USP3 binds ZFAND6. 1 / 1

1 |

---

➶

biogrid

No evidence text available

22939629

WDTC1 affects USP3

1 |

USP3 binds WDTC1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

Vitamin E affects USP3

1 |

Vitamin E decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

19244175

USP44 affects USP3

| 1

USP44 activates USP3. 1 / 1

| 1

---

➶

reach

"Similarly to USP3, USP44 can target the most common H2AK119ub mark and displace RNF168 and 53BP1 from IRIF [60,61]."

26882840

USP3 affects ubiquitination TRAF6

| 1

USP3 inhibits ubiquitination TRAF6. 1 / 1

| 1

---

➶

eidos

"Moreover , USP3 can reverse the ubiquitination of TRAF6 ( tumor necrosis factor-receptor-associated factor 6 ) , which intermediates the signals from inflammatory cytokines to NF-kappaB activation , thus blocking IL-1beta-induced chondrocyte apoptosis [ 27 ] ."

34216299

USP3 affects span

| 1

USP3 inhibits span. 1 / 1

| 1

---

➶

reach

"Loss of USP3 leads to shorter life span and increased cancer incidence."

25113974

USP3 affects snail

| 1

USP3 leads to the deubiquitination of snail. 1 / 1

| 1

---

➶

reach

"USP3 mediates the deubiquitination of snail family transcriptional repressor 1 (SNAIL1)."

35739527

USP3 affects signaling

| 1

USP3 inhibits signaling. 1 / 1

| 1

---

➶

sparser

"Notably, USP3 inhibited signaling by the constitutively active, truncated form of RIG-I (N-RIG-I), but did not inhibit MAVS signaling."

28295214

USP3 affects sensitized response

| 1

USP3 activates sensitized response. 1 / 1

| 1

---

➶

eidos

"One possible explanation for this phenotype is that the absence of USP3 could lead to hyper-ubiquitination of H2A and H2B , resulting in a sensitized DNA damage response which eventually leads to genome instability ."

30544989

USP3 affects recruitment

| 1

USP3 inhibits recruitment. 1 / 1

| 1

---

➶

sparser

"USP3 inhibited recruitment of RNF168, 53BP1, and RAP80 to DNA lesions, through a mechanism dependent on its catalytic activity ( Doil et al., 2009 )."

24647359

USP3 affects proteasome complex disassembly

| 1

USP3 activates proteasome complex disassembly. 1 / 1

| 1

---

➶

reach

"In this mechanism, USP3 leads to suppression of Aurora A ubiquitination, resulting less proteasome degradation."

34758762

USP3 affects pluripotent morphology hESCs

| 1

USP3 activates pluripotent morphology hESCs. 1 / 1

| 1

---

➶

eidos

"The depletion of USP3 leads to a decrease in Oct4 protein level and loss of pluripotent morphology in hESCs ."

34070420

USP3 affects phorbol 13-acetate 12-myristate

| 1

USP3 activates phorbol 13-acetate 12-myristate. 1 / 1

| 1

---

➶

reach

"Taken together, our data indicate that USP3 promotes TPA mediated leukemia cell differentiation via regulating H2AK119ub levels."

31700696

USP3 affects peptidase activity

| 1

Peptidase activity binds USP3. 1 / 1

| 1

---

➶

sparser

"The two proteins form a nuclear coactivator complex which stimulates transcription of the ubiquitin specific peptidase 3, USP3."

33767157

USP3 affects nuclear factor kappaB

| 1

USP3 inhibits nuclear factor kappaB. 1 / 1

| 1

---

➶

reach

"USP3 overexpression inhibited IL-1beta induced chondrocytes apoptosis and nuclear factor kappaB (NF-kappaB) activation."

31056254

USP3 affects migration

| 1

USP3 activates migration. 1 / 1

| 1

---

➶

eidos

"Upregulation of USP3 restored MKN-28 and MGC-803 cell proliferation and migration after overexpression of miR-224-5p ."

32376451

USP3 affects microRNA-146-5p

| 1

USP3 binds microRNA-146-5p. 1 / 1

| 1

---

➶

reach

"In addition, the interaction between microRNA-146-5p and USP3 was examined by luciferase reporting assay."

34917199

USP3 affects miR-224

| 1

USP3 activates miR-224. 1 / 1

| 1

---

➶

reach

"USP3 and SMAD4 were direct targets of miR-224."

28655924

USP3 affects metastasis esophageal squamous cell carcinoma

| 1

USP3 activates metastasis esophageal squamous cell carcinoma. 1 / 1

| 1

---

➶

eidos

"Here , we report that ubiquitin specific peptidase 3 ( USP3 ) promotes proliferation and metastasis of esophageal squamous cell carcinoma ( ESCC ) cells by mediating deubiquitination of Aurora A. Analysis of human clinical samples indicated that USP3 and Aurora A are highly expressed in ESCC ."

34758762

USP3 affects hyper-ubiquitination H2A H2B

| 1

USP3 inhibits hyper-ubiquitination H2A H2B. 1 / 1

| 1

---

➶

eidos

"One possible explanation for this phenotype is that the absence of USP3 could lead to hyper-ubiquitination of H2A and H2B , resulting in a sensitized DNA damage response which eventually leads to genome instability ."

30544989

USP3 affects hsa_circ_0017639

| 1

USP3 activates hsa_circ_0017639. 1 / 1

| 1

---

➶

reach

"Bioinformatic and luciferase reporter assays validated that miR-224-5p and USP3 were downstream targets of hsa_circ_0017639."

32376451

USP3 affects hoxA

| 1

USP3 binds PDS5B and hoxA. 1 / 1

| 1

---

➶

sparser

"In conclusion, we found an associated lncRNA network that regulates the tumour cell development network, consisting of lncRNA HOXA‐AS3, miR‐455‐5p and USP3."

32918360

USP3 affects homeostatic process

| 1

USP3 activates homeostatic process. 1 / 1

| 1

---

➶

reach

"Together, these data demonstrate that USP3 deficient HSCs are more sensitive to genotoxic stress in vivo and in vitro and support the notion that unresolved DNA damage affects the functional integrity of Usp3Delta and Delta HSCs in homeostasis, as well as upon aging and stress."

25113974

USP3 affects histones H2B

| 1

USP3 deubiquitinates histones H2B. 1 / 1

| 1

---

➶

reach

"Several DUBs have been implicated in histone deubiquitination, including USP3, USP12, USP22, and USP46, which deubiquitinate both histones H2A and histones H2B [XREF_BIBR]."

33327527

USP3 affects hemopoiesis

| 1

USP3 activates hemopoiesis. 1 / 1

| 1

---

➶

reach

"Collectively, these findings experimentally support a predominantly cell-autonomous function of USP3 in HSCs under both homeostatic conditions and stress induced hematopoiesis."

25113974

USP3 affects have interaction RIG-I

| 1

USP3 activates have interaction RIG-I. 1 / 1

| 1

---

➶

eidos

"More specifically , Poly ( I :C ) ( LMW ) stimulation leads USP3 to have a strong interaction with RIG-I , but a weak one with MDA5 , while Poly ( I :C ) ( HMW ) stimulation leads USP3 to have a strong interaction with MDA5 , but a weak one with RIG-I [ 25 ] ."

31208841

USP3 affects have interaction MDA5

| 1

USP3 activates have interaction MDA5. 1 / 1

| 1

---

➶

eidos

"More specifically , Poly ( I :C ) ( LMW ) stimulation leads USP3 to have a strong interaction with RIG-I , but a weak one with MDA5 , while Poly ( I :C ) ( HMW ) stimulation leads USP3 to have a strong interaction with MDA5 , but a weak one with RIG-I [ 25 ] ."

31208841

USP3 affects growth cervical tumor xenografts mice

| 1

USP3 activates growth cervical tumor xenografts mice. 1 / 1

| 1

---

➶

eidos

"USP3 depletion reduces the Cdc25A protein level , resulting in a significant delay in cell-cycle progression , and reduces the growth of cervical tumor xenografts in nude mice ."

32415280

USP3 affects glycolytic process

| 1

USP3 activates glycolytic process. 1 / 1

| 1

---

➶

reach

"Glycolysis in GBC cells ws promoted by the USP3 overexpression and inhibited bye USP3 downregulation."

36792297

USP3 affects function

| 1

USP3 inhibits function. 1 / 1

| 1

---

➶

sparser

"USP3 and USP21 inhibit MDA5 function via deubiquitination xref , xref , and proteins such as dihydroxyacetone kinase (DAK), Atg5-Atg-12, NLRC5, and TRIM13 interact with and inhibit MDA5 (Table xref ) xref , xref – xref ."

31827068

USP3 affects deubiquitination snail family transcriptional

| 1

USP3 inhibits deubiquitination snail family transcriptional. 1 / 1

| 1

---

➶

eidos

"USP3 mediates the deubiquitination of snail family transcriptional repressor 1 ( SNAIL1 ) ."

35739527

USP3 affects defects

| 1

USP3 inhibits defects. 1 / 1

| 1

---

➶

eidos

"Together these observations suggest that knockdown of Usp3 causes defects in replication that cause activation of the ATM / ATR DNA damage response and delays progression though the S-phase ( 157 ) ."

19489724

USP3 affects chondrocyte apoptosis induced interleukin IL beta

| 1

USP3 inhibits chondrocyte apoptosis induced interleukin IL beta. 1 / 1

| 1

---

➶

eidos

"Despite it has been demonstrated that ubiquitin-specific protease 3 ( USP3 ) inhibits chondrocyte apoptosis induced by interleukin ( IL ) -1 beta , the role of USP3 / SIRT3 / FOXO3 in the senescence of chondrocytes in OA is unclear ."

34847835

USP3 affects cell

| 1

USP3 activates cell. 1 / 1

| 1

---

➶

eidos

"Both CCK-8 and EdU assays consistently indicated that USP3 knockdown markedly reduced the proliferation of HCC cells ( P < 0.05 , Figures 2B , C ) ."

33195243

USP3 affects activation IFN signaling pathways

| 1

USP3 inhibits activation IFN signaling pathways. 1 / 1

| 1

---

➶

eidos

"For example , USP3 , USP38 , and USP19 target RIG-I , TBK1 , and TRAF3 for deubiquitination , respectively , thereby blocking the activation of type I IFN signaling pathways ( Cui et al ., 2014 ; Gu et al ., 2017 ; Lin et al ., 2016 ) ."

30408499

USP3 affects ZFAND6

1 |

USP3 binds ZFAND6. 1 / 1

1 |

---

➶

biogrid

No evidence text available

22939629

USP3 affects WDTC1

1 |

USP3 binds WDTC1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects Virus Diseases

| 1

USP3 inhibits Virus Diseases. 1 / 1

| 1

---

➶

reach

"We found that USP3 restricts HIV-1 viral infections by increasing the expression of the antiviral factor A3G."

36574218

USP3 affects Vif

| 1

USP3 deubiquitinates Vif. 1 / 1

| 1

---

➶

reach

"Further investigation found that USP3 stabilized 90% to 95% of A3G expression by deubiquitinating Vif-mediated polyubiquitination and blocking its degradation in an enzyme-dependent manner."

36574218

USP3 affects USP3

| 1

USP3 increases the amount of USP3. 1 / 1

| 1

---

➶

reach

"Results of the rescue assay confirmed that PKLR knockdown suppressed USP3-induced oncogenic activity in USP3 overexpressed GBC cells."

36792297

USP3 affects USP16

| 1

USP16 binds USP3 and H2AC20. 1 / 1

| 1

---

➶

sparser

"Both USP3 and USP16 are also associated with H2A deubiquitination following DNA damage processing [ xref , xref ]."

20974139

USP3 affects TXNDC17

1 |

USP3 binds TXNDC17. 1 / 1

1 |

---

➶

biogrid

No evidence text available

22939629

USP3 affects TXLNG

1 |

USP3 binds TXLNG. 1 / 1

1 |

---

➶

biogrid

No evidence text available

22939629

USP3 affects TOR1AIP2

1 |

USP3 binds TOR1AIP2. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects TIRF

| 1

USP3 inhibits TIRF. 1 / 1

| 1

---

➶

eidos

"USP3 did not inhibit MAVS , STING , TBK1 , IRF3 and TIRF , as demonstrated by ISRE-luc activity induction test [ 25 ] ."

31208841

USP3 affects TGFB

| 1

USP3 activates TGFB. 1 / 1

| 1

---

➶

reach

"USP3, which is induced by transforming growth factor-beta 1 (TGF-β1), facilitates the migration and invasion of GC cells by enhancing the deubiquitination and stability of suppressor of Zeste 12 homolog (SUZ12)14."

34512150

USP3 affects SRSF1

| 1

SRSF1 binds USP3 and DDX58. 1 / 1

| 1

---

➶

sparser

"To examine which proteins directly interact with RIG-I, we transfected 293T cells with HA-tagged candidate genes together with Flag-tagged RIG-I. Coimmunoprecipitation and western blot analyses revealed that USP3 and SRSF1 specifically interact with RIG-I in vitro ( xref and xref )."

25658361

USP3 affects SIRT3

| 1

USP3 activates SIRT3. 1 / 1

| 1

---

➶

eidos

"USP3 upregulated SIRT3 to deacetylate FOXO3 and attenuated IL-1beta-induced chondrocyte senescence ."

34847835

USP3 affects SH3BGRL3

1 |

USP3 binds SH3BGRL3. 1 / 1

1 |

---

➶

biogrid

No evidence text available

22939629

USP3 affects SEC23

| 1

USP3 deubiquitinates SEC23. 1 / 1

| 1

---

➶

reach

"Interestingly, other data show that Sec23 is ubiquitylated by Rsp5 and de-ubiquitylated by Usp3/Bre5, with the ubiquitylated form of Sec23 unable to interact with Sec24."

36288698

USP3 affects Radiation, Ionizing

| 1

USP3 inhibits Radiation, Ionizing. 1 / 1

| 1

---

➶

reach

"USP3 overexpression leads to a reduction of steady-state uH2A levels and, strikingly, abrogates the formation of uH2A IR induced foci."

19230794

USP3 affects RSU1

1 |

RSU1 binds USP3. 1 / 1

1 |

---

➶

biogrid

No evidence text available

26344197

USP3 affects RPS15

| 1

USP3 inhibits RPS15. 1 / 1

| 1

---

➶

reach

"USP3, USP21 and CYLD inhibit RIG‐I K63‐linked ubiquitination and activation."

28744851

USP3 affects RNF8

| 1

USP3 inhibits RNF8. 1 / 1

| 1

---

➶

reach

"USP3: Antagonizing RNF8/RNF168 at H2A-Type Histones."

32708614

USP3 affects RLR

| 1

Caspase binds RLR and USP3. 1 / 1

| 1

---

➶

sparser

"We further show that there is no interaction between USP3 and RIG-I-like receptors (RLRs) in unstimulated or uninfected cells, but upon viral infection or ligand stimulation, USP3 binds to the caspase activation recruitment domain of RLRs and then cleaves polyubiquitin chains through cooperation of its zinc-finger Ub-binding domain and USP catalytic domains."

24366338

USP3 affects RIMBP2

1 |

USP3 binds RIMBP2. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects PYCARD

| 1

USP3 deubiquitinates PYCARD. 1 / 1

| 1

---

➶

reach

"USP3 deubiquitinates and stabilizes the adapter protein ASC to regulate inflammasome activation."

36050480

USP3 affects PRRC2B

1 |

USP3 binds PRRC2B. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects PDZK1

1 |

USP3 binds PDZK1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

28514442

USP3 affects PDS5B

| 1

USP3 binds PDS5B and hoxA. 1 / 1

| 1

---

➶

sparser

"In conclusion, we found an associated lncRNA network that regulates the tumour cell development network, consisting of lncRNA HOXA‐AS3, miR‐455‐5p and USP3."

32918360

USP3 affects PARP1

| 1

USP3 inhibits PARP1. 1 / 1

| 1

---

➶

reach

"Importantly, inhibition and/or deletion of PARP1 or USP3 restores transcriptional recovery in XRCC1 -/- cells, highlighting PARP1 and USP3 as possible therapeutic targets in neurological disease."

34811483

USP3 affects Oct4 protein

| 1

USP3 activates Oct4 protein. 1 / 1

| 1

---

➶

eidos

"The depletion of USP3 leads to a decrease in Oct4 protein level and loss of pluripotent morphology in hESCs ."

34070420

USP3 affects NXN

1 |

USP3 binds NXN. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects NT5E

1 |

USP3 binds NT5E. 1 / 1

1 |

---

➶

biogrid

No evidence text available

28514442

USP3 affects NFkappaB

| 1

USP3 inhibits NFkappaB. 1 / 1

| 1

---

➶

reach

"USP3 knockdown induced chondrocytes apoptosis and activated NF-kappaB pathway."

31056254

USP3 affects NANOG

1 |

USP3 deubiquitinates NANOG. 1 / 1

1 |

---

➶

ubibrowser

"Review"

27919796

USP3 affects MYCN protein stability

| 1

USP3 activates MYCN protein stability. 1 / 1

| 1

---

➶

eidos

"We show that increased USP3 levels reduce K-48 - and K-63-linked ubiquitination of MYCN , thus driving up MYCN protein stability ."

33767157

USP3 affects MKN-28 MGC-803 cell

| 1

USP3 activates MKN-28 MGC-803 cell. 1 / 1

| 1

---

➶

eidos

"Upregulation of USP3 restored MKN-28 and MGC-803 cell proliferation and migration after overexpression of miR-224-5p ."

32376451

USP3 affects LRP1

1 |

USP3 binds LRP1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects K63-polyubiquitin chains

| 1

USP3 activates K63-polyubiquitin chains. 1 / 1

| 1

---

➶

reach

"Ubiquitylation events are reversible processes, and, accordingly, several deubiquitylating enzymes, in particular ubiquitin specific peptidase 3 (USP3), USP21 and CYLD lysine 63 deubiquitinase (CYLD), modulate RIG-I signalling by removing K63-polyubiquitin chains, although with unique kinetics."

31792373

USP3 affects K63

| 1

USP3 binds IFIH1, K63, and polyubiquitin. 1 / 1

| 1

---

➶

sparser

"In addition to RIG-I, USP3 also binds MDA5 to remove K63-polyubiquitin chains on the N-terminal CARDs [ xref ]."

28295214

USP3 affects K-63-linked ubiquitination MYCN

| 1

USP3 inhibits K-63-linked ubiquitination MYCN. 1 / 1

| 1

---

➶

eidos

"We show that increased USP3 levels reduce K-48 - and K-63-linked ubiquitination of MYCN , thus driving up MYCN protein stability ."

33767157

USP3 affects K-48

| 1

USP3 inhibits K-48. 1 / 1

| 1

---

➶

eidos

"We show that increased USP3 levels reduce K-48 - and K-63-linked ubiquitination of MYCN , thus driving up MYCN protein stability ."

33767157

USP3 affects IL1

| 1

USP3 inhibits IL1. 1 / 1

| 1

---

➶

reach

"This study demonstrated that USP3 probably attenuated IL-1β-mediated chondrocyte senescence by deacetylating FOXO3 via SIRT3."

34847835

USP3 affects IL-1beta-mediated chondrocyte senescence

| 1

USP3 inhibits IL-1beta-mediated chondrocyte senescence. 1 / 1

| 1

---

➶

eidos

"This study demonstrated that USP3 probably attenuated IL-1beta-mediated chondrocyte senescence by deacetylating FOXO3 via SIRT3 ."

34847835

USP3 affects IL-1beta-induced chondrocyte senescence

| 1

USP3 inhibits IL-1beta-induced chondrocyte senescence. 1 / 1

| 1

---

➶

eidos

"USP3 upregulated SIRT3 to deacetylate FOXO3 and attenuated IL-1beta-induced chondrocyte senescence ."

34847835

USP3 affects IL-1beta-induced chondrocyte apoptosis

| 1

USP3 inhibits IL-1beta-induced chondrocyte apoptosis. 1 / 1

| 1

---

➶

eidos

"Moreover , USP3 can reverse the ubiquitination of TRAF6 ( tumor necrosis factor-receptor-associated factor 6 ) , which intermediates the signals from inflammatory cytokines to NF-kappaB activation , thus blocking IL-1beta-induced chondrocyte apoptosis [ 27 ] ."

34216299

USP3 affects Histone_H2A

| 1

USP3 deubiquitinates Histone_H2A on K13. 1 / 1

| 1

---

➶

trips

"USP3 counteracts RNF168 via deubiquitinating H2A and γH2AX at lysine 13 and 15."

24196443

USP3 affects HSCs knockout mice are chronic genotoxic stress causing

| 1

USP3 inhibits HSCs knockout mice are chronic genotoxic stress causing. 1 / 1

| 1

---

➶

eidos

"Due to the loss of USP3 , HSCs in USP3 knockout mice are under chronic genotoxic stress , causing shortened lifespan and associated functional declines in the hematopoietic stem and progenitor compartment ."

32354135

USP3 affects H3C1

1 |

USP3 binds H3C1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

25281560

USP3 affects H2BK120ub

| 1

USP3 deubiquitinates H2BK120ub. 1 / 1

| 1

---

➶

reach

"Other major DUBs with specificity for histone H2AK119ub over H2BK120ub are BAP1 and USP16, while USP3, USP12, USP22, and USP44 deubiquitinate both H2AK119ub and H2BK120ub, as well as different non histone substrates [XREF_BIBR]."

32344625

USP3 affects H2BC3

1 |

USP3 binds H2BC3. 1 / 1

1 |

---

➶

biogrid

No evidence text available

17980597

USP3 affects H2BC21

1 |

USP3 deubiquitinates H2BC21. 1 / 1

1 |

---

➶

ubibrowser

"USP3 dynamically associates with chromatin and deubiquitinates H2A/H2B in vivo."

17980597

USP3 affects H2BC10

1 |

USP3 deubiquitinates H2BC10. 1 / 1

1 |

---

➶

ubibrowser

"Here we identify the ubiquitin-specific protease 3 USP3 as a deubiquitinating enzyme for uH2A and uH2B."

17980597

USP3 affects H2AK13/15ub

| 1

USP3 deubiquitinates H2AK13/15ub. 1 / 1

| 1

---

➶

reach

"USP3 deubiquitinates H2AK13/15ub as well as H2A119ub in response to DNA damage and affects recruitment of 53BP1 in cells [19,46]."

28624371

USP3 affects H2AK119ub

| 1

USP3 deubiquitinates H2AK119ub. 1 / 1

| 1

---

➶

reach

"Other major DUBs with specificity for histone H2AK119ub over H2BK120ub are BAP1 and USP16, while USP3, USP12, USP22, and USP44 deubiquitinate both H2AK119ub and H2BK120ub, as well as different non histone substrates [XREF_BIBR]."

32344625

USP3 affects H2AC4

1 |

USP3 binds H2AC4. 1 / 1

1 |

---

➶

biogrid

No evidence text available

17980597

USP3 affects H2AC17

1 |

USP3 deubiquitinates H2AC17. 1 / 1

1 |

---

➶

ubibrowser

"USP3 and USP16 function to remove ubiquitin from histone H2A during the DDR"

28778421

USP3 affects H2A119ub

| 1

USP3 deubiquitinates H2A119ub. 1 / 1

| 1

---

➶

reach

"USP3 deubiquitinates H2AK13/15ub as well as H2A119ub in response to DNA damage and affects recruitment of 53BP1 in cells [19,46]."

28624371

USP3 affects GST

| 1

GST binds USP3. 1 / 1

| 1

---

➶

sparser

"In addition, GST pull-down assays were performed to validate the in vitro results showing that GST-USP3 effectively decreased COL9A3 and COL6A5 recombinant proteins in AGS cells."

34930901

USP3 affects GPC1

1 |

USP3 binds GPC1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

28514442

USP3 affects GNAL

1 |

USP3 binds GNAL. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects GNA13

1 |

USP3 binds GNA13. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects GBM progress

| 1

USP3 activates GBM progress. 1 / 1

| 1

---

➶

eidos

"27 , 28 Recent studies have proven that USP3 promotes GBM progress by regulating the EMT process ."

32918360

USP3 affects FUT1

| 1

USP3 binds FUT1. 1 / 1

| 1

---

➶

sparser

"In agreement with the enhanced DNA breaks in MEFs, USP3 loss was further associated with spontaneous DNA damage in HSCs in vivo and in vitro."

25113974

USP3 affects FOXO3

| 1

USP3 deacetylates FOXO3. 1 / 1

| 1

---

➶

reach

"USP3 upregulated SIRT3 to deacetylate FOXO3 and attenuated IL-1β-induced chondrocyte senescence."

34847835

USP3 affects FBXL22

| 1

USP3 binds FBXL22. 1 / 1

| 1

---

➶

sparser

"The PRL and SOX4 protein-coding genes are associated with CASC15 , PPP1R26 is associated with PPP1R26-AS1 , and USP3 and FBXL22 are associated with USP3-AS1 ."

31920530

USP3 affects ELAVL1

1 |

USP3 binds ELAVL1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19322201

USP3 affects EIF4G1

1 |

USP3 binds EIF4G1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects EIF3I

1 |

USP3 binds EIF3I. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects EIF3H

1 |

USP3 binds EIF3H. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects EIF3G

1 |

USP3 binds EIF3G. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects EIF3F

1 |

USP3 binds EIF3F. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects EIF3D

1 |

USP3 binds EIF3D. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects EIF3C

1 |

USP3 binds EIF3C. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects EIF3B

1 |

USP3 binds EIF3B. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects EIF3A

1 |

USP3 binds EIF3A. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects DNA damage checkpoint activation

| 1

USP3 inhibits DNA damage checkpoint activation. 1 / 1

| 1

---

➶

eidos

"Loss of USP3 , which is a de-ubiquitinase for H2A and H2B , also leads to DNA damage checkpoint activation and delayed S-phase progression [ 41 ] ."

30544989

USP3 affects DNA Breaks, Double-Stranded

| 1

USP3 inhibits DNA Breaks, Double-Stranded. 1 / 1

| 1

---

➶

reach

"Knockdown of USP3 alone activates the DSB response, as determined by a number of factors including a delay in S-phase progression, an increased number of DNA breaks and structural abnormalities, ATM/ATR activation, and γH2AX staining [109,132,134]."

32708614

USP3 affects DHX15

1 |

USP3 binds DHX15. 1 / 1

1 |

---

➶

biogrid

No evidence text available

26344197

USP3 affects Cdc25A protein

| 1

USP3 activates Cdc25A protein. 1 / 1

| 1

---

➶

eidos

"USP3 depletion reduces the Cdc25A protein level , resulting in a significant delay in cell-cycle progression , and reduces the growth of cervical tumor xenografts in nude mice ."

32415280

USP3 affects Caspase

| 1

Caspase binds RLR and USP3. 1 / 1

| 1

---

➶

sparser

"We further show that there is no interaction between USP3 and RIG-I-like receptors (RLRs) in unstimulated or uninfected cells, but upon viral infection or ligand stimulation, USP3 binds to the caspase activation recruitment domain of RLRs and then cleaves polyubiquitin chains through cooperation of its zinc-finger Ub-binding domain and USP catalytic domains."

24366338

USP3 affects CBR3

1 |

USP3 binds CBR3. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

USP3 affects CARD

| 1

USP3 binds CARD. 1 / 1

| 1

---

➶

reach

"Upon viral infection, USP3 directly interacts with the CARD of RIG-I and cleaves the K63 linked polyUb chains, but not the K48 linked polyUb chain, thus converting RIG-I to its inactive form [XREF_BIBR]."

33138315

USP3 affects CARD domain

| 1

USP3 binds CARD domain and polyubiquitin chains. 1 / 1

| 1

---

➶

reach

"[164] Upon viral infection or ligand stimulation, USP3 binds to the CARD domain of RIG-I and then removes its polyubiquitin chains through both the zinc-finger Ub binding domain and USP catalytic domains."

33511009

USP3 affects CALM1

| 1

USP3 binds CALM1. 1 / 1

| 1

---

➶

sparser

"To determine which SVBV-encoded protein was a translation trans-activator, we cloned individual SVBV-US ORFs into the pCAM-2300 vector to produce pCAM-USP1, pCAM-USP2, pCAM-USP3, pCAM-USP4, pCAM-USP5, and pCAM-USP6."

30558257

USP3 affects BRCA1

| 1

USP3 inhibits BRCA1. 1 / 1

| 1

---

➶

reach

"In addition, USP3 overexpression impaired the accumulation of downstream repair factors BRCA1 and 53BP1 at the damage sites in response to both UV and gamma irradiation."

24196443

USP3 affects BAP1

| 1

USP3 binds KLF5 and BAP1. 1 / 1

| 1

---

➶

sparser

"Both BAP1 and USP3 bind to and stabilize KLF5 via deubiquitination [ xref , xref ], indicating the possible regulatory role of DUBs in cancer proliferation."

32268558

USP3 affects Aurora A ubiquitination

| 1

USP3 inhibits Aurora A ubiquitination. 1 / 1

| 1

---

➶

eidos

"In this mechanism , USP3 leads to suppression of Aurora A ubiquitination , resulting less proteasome degradation ."

34758762

USP3 affects ATP6V1H

1 |

USP3 binds ATP6V1H. 1 / 1

1 |

---

➶

biogrid

No evidence text available

22939629

USP3 affects PGL3-USP3 3'UTR

| 1

USP3 activates PGL3-USP3 3'UTR. 1 / 1

| 1

---

➶

reach

"Similarly, overexpression of USP3 or SMAD4 3 ' UTR led to significantly increased activities on the PGL3-USP3 3 ' UTR and PGL3-SMAD4 3 ' UTR reporter constructs."

28655924

USP3 affects PGL3-SMAD4 3'UTR reporter constructs

| 1

USP3 activates PGL3-SMAD4 3'UTR reporter constructs. 1 / 1

| 1

---

➶

reach

"Similarly, overexpression of USP3 or SMAD4 3 ' UTR led to significantly increased activities on the PGL3-USP3 3 ' UTR and PGL3-SMAD4 3 ' UTR reporter constructs."

28655924

USP16 affects USP3

| 1

USP16 binds USP3 and H2AC20. 1 / 1

| 1

---

➶

sparser

"Both USP3 and USP16 are also associated with H2A deubiquitination following DNA damage processing [ xref , xref ]."

20974139

USP1 affects USP3

| 1

USP1 activates USP3. 1 / 1

| 1

---

➶

reach

"In addition, DUBs involved in DNA damage signaling are USP1 that targets PCNA (proliferating cell nuclear antigen) [76], FANCD2 and FANCI (the Fanconi anemia proteins) [93,94], and USP3 and USP16 that[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

21763684

UBC affects DDX58

| 1

UBC binds USP3 and DDX58. 1 / 1

| 1

---

➶

sparser

"Because it was recently shown that USP3 interacts with RIG-I to deubiquitinate K63-linked poly-ubiquitin chains on RIG-I and inhibit type I IFN pathways [ xref ], we focused on SRSF1 for further experiments."

25658361

TXNDC17 affects USP3

1 |

USP3 binds TXNDC17. 1 / 1

1 |

---

➶

biogrid

No evidence text available

22939629

TXLNG affects USP3

1 |

USP3 binds TXLNG. 1 / 1

1 |

---

➶

biogrid

No evidence text available

22939629

TOR1AIP2 affects USP3

1 |

USP3 binds TOR1AIP2. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

STING1 affects USP3

1 |

USP3 binds STING1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

27801882

SRSF1 affects USP3

| 1

SRSF1 binds USP3 and DDX58. 1 / 1

| 1

---

➶

sparser

"To examine which proteins directly interact with RIG-I, we transfected 293T cells with HA-tagged candidate genes together with Flag-tagged RIG-I. Coimmunoprecipitation and western blot analyses revealed that USP3 and SRSF1 specifically interact with RIG-I in vitro ( xref and xref )."

25658361

SP1 affects USP3

1 |

SP1 decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:msigdb

No evidence text available

SMO affects USP3

| 1

SMO decreases the amount of USP3. 1 / 1

| 1

---

➶

reach

"Moreover, we found that Smo reduced Claspin polyubiquitination and proteasomal degradation by promoting USP3 transcription."

31900278

SH3BGRL3 affects USP3

1 |

USP3 binds SH3BGRL3. 1 / 1

1 |

---

➶

biogrid

No evidence text available

22939629

RSU1 affects USP3

1 |

RSU1 binds USP3. 1 / 1

1 |

---

➶

biogrid

No evidence text available

26344197

RNAi affects USP3

| 1

RNAi inhibits USP3. 1 / 1

| 1

---

➶

reach

"Depletion of Usp3 by RNAi lead to a increase in the levels of uH2A and to a lesser extent uH2B demonstrating that it regulates the level of histone ubiquitination in vivo."

19489724

RLR affects USP3

| 1

Caspase binds RLR and USP3. 1 / 1

| 1

---

➶

sparser

"We further show that there is no interaction between USP3 and RIG-I-like receptors (RLRs) in unstimulated or uninfected cells, but upon viral infection or ligand stimulation, USP3 binds to the caspase activation recruitment domain of RLRs and then cleaves polyubiquitin chains through cooperation of its zinc-finger Ub-binding domain and USP catalytic domains."

24366338

RIMBP2 affects USP3

1 |

USP3 binds RIMBP2. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

REPIN1 affects USP3

1 |

REPIN1 decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:msigdb

No evidence text available

RBM4 affects USP3

| 1

RBM4 activates USP3. 1 / 1

| 1

---

➶

reach

"In addition, the result of Dual-Luciferase reporter assay demonstrated that USP3 can be targeted by RBM4."

32271432

Poly :C affects USP3

| 1

Poly :C activates USP3. 1 / 1

| 1

---

➶

eidos

"More specifically , Poly ( I :C ) ( LMW ) stimulation leads USP3 to have a strong interaction with RIG-I , but a weak one with MDA5 , while Poly ( I :C ) ( HMW ) stimulation leads USP3 to have a strong interaction with MDA5 , but a weak one with RIG-I [ 25 ] ."

31208841

Poly :C HMW affects USP3

| 1

Poly :C HMW activates USP3. 1 / 1

| 1

---

➶

eidos

"More specifically , Poly ( I :C ) ( LMW ) stimulation leads USP3 to have a strong interaction with RIG-I , but a weak one with MDA5 , while Poly ( I :C ) ( HMW ) stimulation leads USP3 to have a strong interaction with MDA5 , but a weak one with RIG-I [ 25 ] ."

31208841

PRRC2B affects USP3

1 |

USP3 binds PRRC2B. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

PDZK1 affects USP3

1 |

USP3 binds PDZK1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

28514442

PDS5B affects hoxA

| 1

USP3 binds PDS5B and hoxA. 1 / 1

| 1

---

➶

sparser

"In conclusion, we found an associated lncRNA network that regulates the tumour cell development network, consisting of lncRNA HOXA‐AS3, miR‐455‐5p and USP3."

32918360

PARP1 affects USP3

| 1

PARP1 inhibits USP3. 1 / 1

| 1

---

➶

reach

"Importantly, inhibition and/or deletion of PARP1 or USP3 restores transcriptional recovery in XRCC1 -/- cells, highlighting PARP1 and USP3 as possible therapeutic targets in neurological disease."

34811483

NXN affects USP3

1 |

USP3 binds NXN. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

NT5E affects USP3

1 |

USP3 binds NT5E. 1 / 1

1 |

---

➶

biogrid

No evidence text available

28514442

NR1H4 affects USP3

1 |

NR1H4 decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:msigdb

No evidence text available

NANOG affects USP10, USP16, USP3, USP37, USP44, and USP7

| 1

USP10 binds USP16, USP3, USP7, USP37, USP44, and NANOG. 1 / 1

| 1

---

➶

sparser

"USP25, USP44, USP49, and USP7 bind to the Sox2 promoter, while USP10, USP16, USP3, USP37, USP44, and USP7 bind to the Nanog promoter."

26880980

MYOD1 affects USP3

1 |

MYOD1 decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:msigdb

No evidence text available

MYCN affects USP3

| 1

USP3 binds MYCN. 1 / 1

| 1

---

➶

sparser

"USP3 interacts directly with its protein targets, which prompted us to examine whether MYCN also bound USP3 directly."

33767157

LRP1 affects USP3

1 |

USP3 binds LRP1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

L-methionine affects USP3

1 |

L-methionine methylates USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

20938992

KLF5 affects BAP1

| 1

USP3 binds KLF5 and BAP1. 1 / 1

| 1

---

➶

sparser

"Both BAP1 and USP3 bind to and stabilize KLF5 via deubiquitination [ xref , xref ], indicating the possible regulatory role of DUBs in cancer proliferation."

32268558

K63 affects polyubiquitin

| 1

USP3 binds IFIH1, K63, and polyubiquitin. 1 / 1

| 1

---

➶

sparser

"In addition to RIG-I, USP3 also binds MDA5 to remove K63-polyubiquitin chains on the N-terminal CARDs [ xref ]."

28295214

K 7174 affects USP3

1 |

K 7174 increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

24086573

IL6 affects USP3

| 1

IL6 activates USP3. 1 / 1

| 1

---

➶

sparser

"111 GAL Galanin rs2156464 Signaling through either type 1 or type 2 receptors, GAL has numerous anti-inflammatory effects;[112][113][114][115] Consistent with PATHOS-D, multiple lines of evidence indicate GAL signaling is reduced in MDD;[116][117][118] GMAP, which is produced by cleavage of the same precursor as galanin, has direct antifungal activity 119 rs11531570 PDK4 gene expression is upregulated by IFN-a and by LPS and contributes to muscle glycogen breakdown and lactate accumulation; 120,121 conversely, PDK4 is inhibited by TNF-a via p38 MAPK and NF-kB signaling, leading to increased glucose oxidation;122 anti-inflammatory omega-3 fatty acids increase PDK4 in immature dendritic cells via enhanced PPAR-g signaling 123NPM1Nucleophosmin rs11134697 Functions as an endogenous 'alarmin' that activates proinflammatory cytokines;124-126 identified as a host virulence factor in viral infection; 127,128 may aid in HIV and HSV1 virus dispersal within cells; 129,130 complexes with, and inhibits, PKR, which has important antiviral properties 131 rs7183892 Embedding of USP genes in the copy number variable b-defensin cluster on chromosome 8p23.1 suggests a close tie with innate immunity; 132 USP3 is activated by IL-4 and IL-6 and has antiproliferative and apoptotic properties; 133 highly homologous USP17 necessary for type I IFN production in response to virus infection; 134 rs11531570 No specific immune or host defense functions identified."

22290120

IL4 affects USP3

| 1

IL4 activates USP3. 1 / 1

| 1

---

➶

sparser

"111 GAL Galanin rs2156464 Signaling through either type 1 or type 2 receptors, GAL has numerous anti-inflammatory effects;[112][113][114][115] Consistent with PATHOS-D, multiple lines of evidence indicate GAL signaling is reduced in MDD;[116][117][118] GMAP, which is produced by cleavage of the same precursor as galanin, has direct antifungal activity 119 rs11531570 PDK4 gene expression is upregulated by IFN-a and by LPS and contributes to muscle glycogen breakdown and lactate accumulation; 120,121 conversely, PDK4 is inhibited by TNF-a via p38 MAPK and NF-kB signaling, leading to increased glucose oxidation;122 anti-inflammatory omega-3 fatty acids increase PDK4 in immature dendritic cells via enhanced PPAR-g signaling 123NPM1Nucleophosmin rs11134697 Functions as an endogenous 'alarmin' that activates proinflammatory cytokines;124-126 identified as a host virulence factor in viral infection; 127,128 may aid in HIV and HSV1 virus dispersal within cells; 129,130 complexes with, and inhibits, PKR, which has important antiviral properties 131 rs7183892 Embedding of USP genes in the copy number variable b-defensin cluster on chromosome 8p23.1 suggests a close tie with innate immunity; 132 USP3 is activated by IL-4 and IL-6 and has antiproliferative and apoptotic properties; 133 highly homologous USP17 necessary for type I IFN production in response to virus infection; 134 rs11531570 No specific immune or host defense functions identified."

22290120

IFIH1 affects carD

| 1

USP3 binds IFIH1 and carD. 1 / 1

| 1

---

➶

sparser

"While the deubiquitinase USP3 inhibits MDA5 as well as RIG-I, it is thought that this may be due to USP3 directly binding the MDA5 CARD domain to prevent RNA filamentation (284)."

31379819

IFIH1 affects USP3, and polyubiquitin

| 1

USP3 binds IFIH1 and polyubiquitin. 1 / 1

| 1

---

➶

sparser

"USP3 may bind to MDA5 via unanchored polyUb chains and then deubiquitinate it [ xref ]."

33138315

IFIH1 affects USP3, and carD

| 1

USP3 binds IFIH1 and carD. 1 / 1

| 1

---

➶

sparser

"While the deubiquitinase USP3 inhibits MDA5 as well as RIG-I, it is thought that this may be due to USP3 directly binding the MDA5 CARD domain to prevent RNA filamentation (284)."

31379819

IFIH1 affects K63, USP3, and polyubiquitin

| 1

USP3 binds IFIH1, K63, and polyubiquitin. 1 / 1

| 1

---

➶

sparser

"In addition to RIG-I, USP3 also binds MDA5 to remove K63-polyubiquitin chains on the N-terminal CARDs [ xref ]."

28295214

Homozygote affects USP3

| 1

Homozygote inhibits USP3. 1 / 1

| 1

---

➶

eidos

"However , three homozygotes failed to fully induce USP3 during differentiation ."

34136489

Histone_H2B affects USP3

| 1

Histone_H2B binds USP3. 1 / 1

| 1

---

➶

reach

"In the DDR, USP3 dynamically interacts with chromatin and deubiquitinates H2A and H2B."

26882840

Histone affects USP3

| 1

Histone binds USP3. 1 / 1

| 1

---

➶

sparser

"USP3 does in fact interact with ubiquitinated H2A-type histones, and mouse embryonic fibroblasts isolated from USP3-null mice express increased levels of ubiquitinated H2A [ xref ]."

32708614

HOXA-AS3 affects USP3

| 1

HOXA-AS3 activates USP3. 1 / 1

| 1

---

➶

reach

"LncRNA HOXA-AS3 promotes the malignancy of glioblastoma through regulating miR-455-5p/USP3 axis."

32918360

H3C1 affects USP3

1 |

USP3 binds H3C1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

25281560

H2BC3 affects USP3

1 |

USP3 binds H2BC3. 1 / 1

1 |

---

➶

biogrid

No evidence text available

17980597

H2AX affects USP3

| 1

USP3 binds H2AX. 1 / 1

| 1

---

➶

sparser

"These effects may be related to a functional interaction between USP3 and the isoform g-H2AX which accumulates at doubled stranded breaks."

21513801

H2AC4 affects USP3

1 |

USP3 binds H2AC4. 1 / 1

1 |

---

➶

biogrid

No evidence text available

17980597

H2AC20 affects USP3

| 1

USP16 binds USP3 and H2AC20. 1 / 1

| 1

---

➶

sparser

"Both USP3 and USP16 are also associated with H2A deubiquitination following DNA damage processing [ xref , xref ]."

20974139

GST affects USP3

| 1

GST binds USP3. 1 / 1

| 1

---

➶

sparser

"In addition, GST pull-down assays were performed to validate the in vitro results showing that GST-USP3 effectively decreased COL9A3 and COL6A5 recombinant proteins in AGS cells."

34930901

GPC1 affects USP3

1 |

USP3 binds GPC1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

28514442

GNAL affects USP3

1 |

USP3 binds GNAL. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

GNA13 affects USP3

1 |

USP3 binds GNA13. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

FUT1 affects USP3

| 1

USP3 binds FUT1. 1 / 1

| 1

---

➶

sparser

"In agreement with the enhanced DNA breaks in MEFs, USP3 loss was further associated with spontaneous DNA damage in HSCs in vivo and in vitro."

25113974

FOXO1 affects USP3

1 |

FOXO1 decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:msigdb

No evidence text available

FOXJ1 affects USP3

1 |

FOXJ1 decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:msigdb

No evidence text available

FOXF2 affects USP3

1 |

FOXF2 decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:msigdb

No evidence text available

FBXL22 affects USP3

| 1

USP3 binds FBXL22. 1 / 1

| 1

---

➶

sparser

"The PRL and SOX4 protein-coding genes are associated with CASC15 , PPP1R26 is associated with PPP1R26-AS1 , and USP3 and FBXL22 are associated with USP3-AS1 ."

31920530

Enterolactone affects USP3

1 |

Enterolactone decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

19167446

ETV7 affects USP3

1 |

ETV7 decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:msigdb

No evidence text available

ETS1 affects USP3

1 |

ETS1 decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:msigdb

No evidence text available

ESRRA affects USP3

1 |

ESRRA decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:msigdb

No evidence text available

ELK1 affects USP3

1 |

ELK1 decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:msigdb

No evidence text available

ELAVL1 affects USP3

1 |

USP3 binds ELAVL1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19322201

EIF4G1 affects USP3

1 |

USP3 binds EIF4G1. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

EIF3I affects USP3

1 |

USP3 binds EIF3I. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

EIF3H affects USP3

1 |

USP3 binds EIF3H. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

EIF3G affects USP3

1 |

USP3 binds EIF3G. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

EIF3F affects USP3

1 |

USP3 binds EIF3F. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

EIF3D affects USP3

1 |

USP3 binds EIF3D. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

EIF3C affects USP3

1 |

USP3 binds EIF3C. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

EIF3B affects USP3

1 |

USP3 binds EIF3B. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

EIF3A affects USP3

1 |

USP3 binds EIF3A. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

Delta(9)-tetrahydrocannabinol affects USP3

1 |

Delta(9)-tetrahydrocannabinol increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

18454173

DHX15 affects USP3

1 |

USP3 binds DHX15. 1 / 1

1 |

---

➶

biogrid

No evidence text available

26344197

DDX58 affects UBC, and USP3

| 1

UBC binds USP3 and DDX58. 1 / 1

| 1

---

➶

sparser

"Because it was recently shown that USP3 interacts with RIG-I to deubiquitinate K63-linked poly-ubiquitin chains on RIG-I and inhibit type I IFN pathways [ xref ], we focused on SRSF1 for further experiments."

25658361

DDX58 affects SRSF1, and USP3

| 1

SRSF1 binds USP3 and DDX58. 1 / 1

| 1

---

➶

sparser

"To examine which proteins directly interact with RIG-I, we transfected 293T cells with HA-tagged candidate genes together with Flag-tagged RIG-I. Coimmunoprecipitation and western blot analyses revealed that USP3 and SRSF1 specifically interact with RIG-I in vitro ( xref and xref )."

25658361

Caspase affects USP3

| 1

Caspase binds RLR and USP3. 1 / 1

| 1

---

➶

sparser

"We further show that there is no interaction between USP3 and RIG-I-like receptors (RLRs) in unstimulated or uninfected cells, but upon viral infection or ligand stimulation, USP3 binds to the caspase activation recruitment domain of RLRs and then cleaves polyubiquitin chains through cooperation of its zinc-finger Ub-binding domain and USP catalytic domains."

24366338

CBR3 affects USP3

1 |

USP3 binds CBR3. 1 / 1

1 |

---

➶

biogrid

No evidence text available

19615732

CARD affects USP3

| 1

USP3 binds CARD. 1 / 1

| 1

---

➶

reach

"Upon viral infection, USP3 directly interacts with the CARD of RIG-I and cleaves the K63 linked polyUb chains, but not the K48 linked polyUb chain, thus converting RIG-I to its inactive form [XREF_BIBR]."

33138315

CARD domain affects polyubiquitin chains

| 1

USP3 binds CARD domain and polyubiquitin chains. 1 / 1

| 1

---

➶

reach

"[164] Upon viral infection or ligand stimulation, USP3 binds to the CARD domain of RIG-I and then removes its polyubiquitin chains through both the zinc-finger Ub binding domain and USP catalytic domains."

33511009

CALM1 affects USP3

| 1

USP3 binds CALM1. 1 / 1

| 1

---

➶

sparser

"To determine which SVBV-encoded protein was a translation trans-activator, we cloned individual SVBV-US ORFs into the pCAM-2300 vector to produce pCAM-USP1, pCAM-USP2, pCAM-USP3, pCAM-USP4, pCAM-USP5, and pCAM-USP6."

30558257

BRCC36 affects OTUB1, PSMD14, RNF8, USP16, and USP3

| 1

RNF8 binds USP16, USP3, PSMD14, OTUB1, and BRCC36. 1 / 1

| 1

---

➶

sparser

"The DUBs USP3, USP16, BRCC36, POH1, and OTUB1 are associated with negative regulation of the RNF8 pathway, with USP3 and USP16 being first linked to this pathway through their ability to oppose H2A ub[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

23416108

BAP1 affects USP3

| 1

USP3 binds KLF5 and BAP1. 1 / 1

| 1

---

➶

sparser

"Both BAP1 and USP3 bind to and stabilize KLF5 via deubiquitination [ xref , xref ], indicating the possible regulatory role of DUBs in cancer proliferation."

32268558

BACH2 affects USP3

1 |

BACH2 decreases the amount of USP3. 1 / 1

1 |

---

➶

biopax:msigdb

No evidence text available

Antirheumatic Agents affects USP3

1 |

Antirheumatic Agents decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

24449571

Air Pollutants affects USP3

1 |

Air Pollutants decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

21757418

ATP6V1H affects USP3

1 |

USP3 binds ATP6V1H. 1 / 1

1 |

---

➶

biogrid

No evidence text available

22939629

ALYREF affects USP3

| 1

USP3 binds ALYREF. 1 / 1

| 1

---

➶

sparser

"Here, we have described a mechanism for MYCN degradation in MYCN-dependent neuroblastoma, regulated by the ALYREF-USP3 signal."

33767157

4-hydroxyphenyl retinamide affects USP3

1 |

4-hydroxyphenyl retinamide increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

28973697

17alpha-ethynylestradiol affects USP3

1 |

17alpha-ethynylestradiol decreases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

23129252

1-phenylpropan-2-amine affects USP3

1 |

1-phenylpropan-2-amine increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

30779732

1-(2-trifluoromethoxyphenyl)-2-nitroethanone affects USP3

1 |

1-(2-trifluoromethoxyphenyl)-2-nitroethanone increases the amount of USP3. 1 / 1

1 |

---

➶

ctd

No evidence text available

25596134