IndraLab

Statements


USP29 affects TWIST1
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USP29 binds TWIST1.
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"As shown in Figure  xref , only the full‐length (FL) and M4 mutant (687‐922aa) region of USP29, but not other mutants, could interact with TWIST1."

sparser
"We next confirmed the endogenous TWIST1USP29 interaction by co‐immunoprecipitation assay."

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"We first examined whether CDK1 regulated the interaction between USP29 with TWIST1."

sparser
"All three sites are outside of the domain (687‐922aa) that mediates the interaction between USP29 and TWIST1."

sparser
"The direct interaction of USP29 and TWIST1 prompted us to examine a potential role of USP29 in the regulation of TWIST1 stability and function."

sparser
"As shown in Figure xref (Supporting Information), the treatment of RO‐3306 in MDA‐MB‐231 cells did not affect the USP29TWIST1 interaction."

sparser
"USP29 directly binds, deubiquitinates, and stabilizes TWIST1, which in turn promotes TWIST1‐driven tumor progression in TNBC."

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"USP29 directly binds, deubiquitinates, and stabilizes TWIST1, which in turn promotes TWIST1‐driven tumor progression in TNBC."

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"The direct interaction of USP29 and TWIST1 prompted us to examine a potential role of USP29 in the regulation of TWIST1 stability and function."

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"In addition, purified GST‐USP29 but not GST pulled down His‐TWIST1 in vitro, indicating the direct interaction between USP29 and TWIST1 (Figure 1D)."
USP29 decreases the amount of TWIST1.
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USP29 decreases the amount of TWIST1. 6 / 6
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"As shown in Figure 2F, USP29 WT but not its catalytically inactive mutant C294S could dramatically decrease the ubiquitination level of TWIST1."

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"Consistently, the depletion of USP29 in MDA‐MB‐231 and BT549 cells markedly increased the ubiquitination level of TWIST1 (Figure 2H, Figure S2I, Supporting Information)."

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"In addition, the overexpression of USP29 dramatically decreased the ubiquitination level of TWIST1, which was markedly blocked by the treatment of RO‐3306 or the depletion of CDK1 (Figure 6B,C)."

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"As shown in Figure 6D and Figure S6B (Supporting Information), the overexpression of USP29 WT could dramatically decrease the ubiquitination level of TWIST1, whereas the 3A mutant failed to do so."

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"We found that the reduced TWIST1 protein level in USP29‐deficient cells was rescued by the reconstitution of USP29 WT, but not the 3A mutant (Figure S6C,D, Supporting Information)."

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"Both USP29 and TWIST1 are overexpressed in TNBC tumor specimens, but not in normal adult breast tissues, highlighting the potential of USP29 as a more druggable target toward TWIST1 stability in TNBC.It is also noteworthy that we can not rule out the possibility of additional mechanisms engaged in UPS29‐regulated tumor progression of TNBC since the ectopic expression of TWIST1 could not completely rescue functional alterations caused by the depletion of USP29 in TNBC cells."
USP29 deubiquitinates TWIST1.
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USP29 deubiquitinates TWIST1. 5 / 5
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"The deubiquitination of TWIST1 catalyzed by USP29 is required for its stabilization and subsequent EMT and CSC functions in TNBC, thereby conferring chemotherapeutic resistance and metastasis."

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"The result of immunofluorescence staining also showed the co‐localization of USP29 with TWIST1 in the nucleus of TNBC cells (Figure 1G).2.2 USP29 Deubiquitinates and Stabilizes TWIST1."

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"These data reveal USP29‐mediated deubiquitination of TWIST1 as a critical mechanism controlling its stability."

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"Next, the specific ubiquitin linkage of TWIST1 deubiquitinated by USP29 was examined."

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"Guan et al. showed that in triple-negative breast cancer, CDK1 leads to the de-ubiquitination of TWIST1 by USP29, which conferred resistance to both cisplatin and paclitaxel [103]."
USP29 activates TWIST1.
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USP29 activates TWIST1. 4 / 4
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"Concerning the well‐established significance of TWIST1 in EMT, CSC self‐renewal, metastasis, and chemo‐resistance, we hypothesized that USP29 might promote these malignant processes through stabilizing TWIST1."

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"Likewise, the overexpression of USP29 WT in MDA‐MB‐231 and BT549 cells dramatically increased the half‐life of TWIST1 compared to the 3A mutant (Figure S6E,F, Supporting Information)."

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"Regarding the fact that both USP29 and CDK1 increase the stability of TWIST1, and CDK1 phosphorylates USP29, We hypothesized that the regulation of TWIST1 by CDK1 might be mediated through the activation of USP29 in TNBC."

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"Taken together, these results suggest that USP29 is a bona fide DUB targeting TWIST1 protein for deubiquitination and stabilization.2.3 USP29 Promotes Tumor Progression of TNBC through Stabilizing TWIST1."
USP29 bound to TWIST1 activates TWIST1. 1 / 1
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"USP29 directly binds, deubiquitinates, and stabilizes TWIST1, which in turn promotes TWIST1‐driven tumor progression in TNBC."
USP29 increases the amount of TWIST1.
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USP29 increases the amount of TWIST1. 4 / 4
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"As shown in Figure 3G–J and Figure S3O–R (Supporting Information), the knockdown of USP29 significantly decreased the expression of TWIST1, and increased cancer cellular sensitivity to these chemotherapeutic agents both in vitro and in vivo, which could be largely reversed by the reconstitution of TWIST1 in USP29‐depleted cells."

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"20 However, we found that the knockdown of USP29 significantly decreased the protein level of TWIST1, but only slightly affected the protein level of Snail1 in MDA‐MB‐231 and BT549 cells (Figure S2D, Supporting Information)."

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"As shown in Figure 2A and Figure S2B (Supporting Information), the knockdown of USP29 significantly decreased the protein level of TWIST1."

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"Of note, the depletion of USP29 in both MDA‐MB‐231 and BT549 cells significantly decreased the expression of TWIST1 and Vimentin, and increased the expression of epithelial marker E‐cadherin (Figure 3A; Figure S3C,D, Supporting Information), while the reconstitution of TWIST1 in USP29‐deficient cells could dramatically rescue such a change (Figure 3A)."
USP29 affects SNAI1
6 | 14 5
USP29 binds SNAI1.
6 | 2 5
6 | 2 5

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"We then moved to investigate the potential interaction between USP29 and Snail1."

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"These observations imply the necessity of USP29 DUB activity in the regulation of Snail1, but the exact roles of USP29 mutations detected in cancer samples require further clarification.To evaluate the interaction between Snail1 and USP29, we carried out a series of co-immunoprecipitation experiments."

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sparser
"Cancer cells hijack the USP29-Snail1 axis to facilitate chemoresistance."

sparser
"Considering the pivotal roles that Snail1 plays during the development of resistance to chemotherapies, we posited that the USP29-Snail1 axis might be exploited by cancer cells in their acquisition of chemoresistance."

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sparser
"USP29 interacts with Snail1 and requires catalytic activity to stabilize Snail1."

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USP29 activates SNAI1.
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USP29 activates SNAI1. 6 / 6
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"18 In addition, USP29 was reported to promote gastric cancer cell migration by cooperating with phosphatase SCP1 to stabilize Snail protein."

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"USP29 enhances chemotherapy-induced stemness in non-small cell lung cancer via stabilizing Snail1 in response to oxidative stress."

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"Deubiquitinase USP29 promotes cell migration by stabilizing Snail protein ."

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"Deubiquitinase USP29 promotes gastric cancer cell migration by cooperating with phosphatase SCP1 to stabilize Snail protein."

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"For example, USP3, USP11, and USP29 stimulate EMT in glioblastoma cells, as well as in ovarian or gastric cancer cells, respectively, by stabilizing Snail [80–82] ."

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"In addition, USP29 overexpression induced by chemotherapy and oxidative stress treatment can promote chemotherapy resistance by hijacking the USP29/Snail1 axis, which is associated with enhanced cancer stem cell properties, poor prognosis and drug resistance in lung cancer (91)."
USP29 increases the amount of SNAI1.
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USP29 increases the amount of SNAI1. 2 / 2
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"Among these DUBs, USP29 and USP13 have been reported to increase Snail1 protein levels in GC cells."

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"USP29 can promote the migration of gastric cancer cells by preventing Snail degradation, thereby maintaining high protein expression of Snail in cancer cells."
USP29 deubiquitinates SNAI1.
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USP29 deubiquitinates SNAI1. 2 / 2
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"USP13, USP29, and USP37 were reported to promote the metastasis of GC by deubiquitinating and stabilizing Snail [35,36,54,59]."

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"Further evidence showed that EMT might be the primary mechanism for the enhanced metastatic property in GC cells: USP13 and USP29 promoted TGF-β1-induced EMT through interacting with and deubiquitinating Snail, while USP3 facilitated this process by deubiquitinating SUZ12 (Wu et al. 2021; Zhang et al. 2022a, b; Qian et al. 2020)."
USP29 ubiquitinates SNAI1.
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USP29 leads to the ubiquitination of SNAI1. 1 / 1
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"Subsequent sub-screening identified 23 DUBs that interact with SNAI1, out of which USP29, USP36, and USP37 upregulate and reduce polyubiquitination of the SNAI1 protein."
| PMC
USP29 decreases the amount of SNAI1.
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USP29-C294A decreases the amount of SNAI1. 1 / 1
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"In addition, the dose-escalating expression of the C294A USP29 mutant effectively suppressed Snail1 expression levels, suggesting a dominant-negative role of the catalytic inactive version that overwhelms the endogenous wild-type USP29 (Fig. 3d)."
ACSL5 affects USP29
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ACSL5 binds USP29.
4 | 4 11
4 | 3 11

sparser
"Thus, targeting USP29-ACSL5 may constitute an effective therapeutic strategy for MASLD."

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"Moreover, USP29 directly interacted with ACSL5 and stabilized ACSL5 via K48-linked deubiquitination."

sparser
"Furthermore, we found that USP29 directly interacted with ACSL5 and upregulated ACSL5 expression."

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"First, the interaction between USP29 and ACSL5 was further confirmed by co-IP (Fig. 7A, B)."

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"Additionally, a GST pull-down assay revealed a direct interaction between USP29 and ACSL5 (Fig. 7C, D)."

sparser
"USP29 interacts with ACSL5 and upregulates ACSL5 expression."
USP29 binds ACSL5 and K48. 1 / 1
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"USP29 directly interacts with ACSL5 and stabilizes ACSL5 through the suppression of K48-linked ubiquitination.."
ACSL5 activates USP29.
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ACSL5 activates USP29. 3 / 3
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"These data suggest that USP29 mediated MASLD by stabilizing ACSL5 and further activating FAO."

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"Intriguingly, the overexpression of ACSL5 reversed the aggravated effects of USP29 knockout on lipid deposition in hepatocytes treated with PAOA (Fig. 6B, C)."

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"To determine whether ACSL5 overexpression also reversed the role of USP29 knockout in MASLD in vivo, ACSL5 was overexpressed in USP29-KO mice by injecting AAV8-ACSL5 (Supplementary Fig. 7A)."
ACSL5 inhibits USP29.
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ACSL5 inhibits USP29. 1 / 1
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"To determine whether ACSL5 overexpression also reversed the role of USP29 knockout in MASLD in vivo, ACSL5 was overexpressed in USP29-KO mice by injecting AAV8-ACSL5 (Supplementary Fig. 7A)."
ACSL5 increases the amount of USP29.
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ACSL5 increases the amount of USP29. 1 / 1
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"ACSL5 protein levels increased in a gradient-dependent manner with the overexpression of USP29 in PAOA-treated hepatocytes (Fig. 5D)."
ACSL5 decreases the amount of USP29.
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ACSL5 decreases the amount of USP29. 1 / 1
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"Notably, ACSL5 knockdown markedly reversed the inhibitory effect of USP29 overexpression on hepatocytes treated with PAOA (Supplementary Fig. 6A–D)."
USP29 affects CGAS
2 1 | 21
USP29 binds CGAS.
2 | 11
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"To test this hypothesis, we conducted an unbiased screening of cGAS interacting DUBs by cotransfection and immunoprecipitation assays as previous established, XREF_BIBR - XREF_BIBR and found that USP25 and USP29 interacted with cGAS in such a system."

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"In addition, USP29 selectively interacted with cGAS but not with other molecules including MITA, RIG-I, MAVS, TBK1 or IRF3 (Supplementary information, Fig.S2d)."

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"Results from endogenous immunoprecipitation assays suggested that USP29 constitutively interacted with cGAS in bone marrow derived macrophages (BMDMs), bone marrow derived dendritic cells (BMDCs), mouse embryonic fibroblasts (MEFs), mouse lung fibroblasts (MLFs) or human monocytic THP-1 cells and their association was potentiated after HSV-1 infection."

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"Interestingly, we found that TRIM38 constitutively interacts with cGAS but not with USP29, whereas USP29 constitutively interacts with cGAS but not with TRIM38 (Supplementary information, Fig.S2h), indicating that TRIM38 and USP29 independently interacts with and regulates cGAS."

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"Together, these data suggest that USP29 interacts with cGAS directly and constitutively in resting cells and after HSV-1 infection."

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"Because USP29 interacted with cGAS and was upregulated by HSV-1 infection, we investigated its role in regulating HSV-1- or cytoplasmic DNA triggered innate immune signaling."

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"Because USP29 interacts with cGAS and its enzyme activity is required for regulating antiviral signaling, we investigated whether USP29 eliminated polyubiquitin chains from cGAS."

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"In addition, we found that TRIM38 and USP29 interacts with cGAS independently, suggesting that TRIM38 mediated sumoylation and USP29 mediated deubiquitination of cGAS cooperatively regulate the stability of cGAS."

No evidence text available

No evidence text available
USP29 binds CGAS and K48. 3 / 3
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"Mechanistically, USP29 constitutively interacts with cGAS, deconjugates K48 linked polyubiquitin chains from cGAS and stabilizes cGAS in uninfected cells or after HSV-1 infection."

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"[208] Recently, a study reported that USP29 interacts with cGAS, hydrolyzes K48 linked polyubiquitin chains on cGAS, and stabilizes cGAS in uninfected cells, or after HSV-1 stimulation."

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"USP29 constitutively interacts with cGAS and deconjugates K48 linked polyubiquitin chains from cGAS in the absence or presence of HSV-1 infection, which stabilizes cGAS and promotes cGAS mediated cellular antiviral responses."
USP29 deubiquitinates CGAS.
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USP29 deubiquitinates CGAS. 3 / 3
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"Mechanistically, <span class="match term0">USP29</span> constitutively interacts with <span class="match term1">cGAS</span>, deconjugates K48-linked polyubiquitin chains from <span class="match term1">cGAS</span> and stabilizes <span class="match term1">cGAS</span> in uninfected cells or after HSV-1 infection"

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"USP27X and USP29 have a similar mechanism for the deubiquitination of cGAS, both preventing the degradation of cGAS via the proteasomal pathway by removing the K48-linked ubiquitin chain of cGAS and maintaining cGAS protein levels."

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"USP29 deubiquitinates cGAS in the presence or absence of HSV-1 infection."
USP29 deubiquitinates CGAS-K464R. 2 / 2
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"In support of this notion, we found that USP29 deubiquitinated cGAS (K464R) but did not affect the ubiquitination of cGAS (K271R)."

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"In this context, we noted that USP29 inhibited ubiquitination of cGAS (K464R) but not cGAS (K271R) after HSV-1 infection, and that knockout of USP29 potentiated ubiquitination of cGAS (K464R) but not cGAS (K271R) before or after HSV-1 infection."
USP29 activates CGAS.
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USP29 activates CGAS. 3 / 3
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"In addition, we found that TRIM38 and USP29 interacts with cGAS independently, suggesting that TRIM38 mediated sumoylation and USP29 mediated deubiquitination of cGAS cooperatively regulate the stability of cGAS."

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"In contrast, cGAS was still degraded in Usp29 m/m MLFs (half-life ~ 8h) after HSV-1 infection, which was due to inefficient induction of cGas transcription and accelerated degradation of basal and newly synthesized cGAS caused by the absence of USP29."

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"These data together suggest that USP29 deconjugates K48 linked polyubiquitin chains from cGAS and thereby inhibits the proteasomal degradation of cGAS."
USP29 increases the amount of CGAS.
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USP29-C298A increases the amount of CGAS. 1 / 1
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"In addition, reconstitution of USP29 but not USP29 (C298A) into Usp29 m/m MEFs restored the protein levels of cGAS with or without HSV-1 infection and cGAMP production after cytoplasmic DNA challenge."
USP29 increases the amount of CGAS. 1 / 1
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"In addition, reconstitution of USP29 but not USP29 (C298A) into Usp29 m/m MEFs restored the protein levels of cGAS with or without HSV-1 infection and cGAMP production after cytoplasmic DNA challenge."
USP29 ubiquitinates CGAS.
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USP29 ubiquitinates CGAS. 1 / 1
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"XREF_BIBR - XREF_BIBR Because USP29 dubiquitinates and stabilizes cGAS, it is worthy to investigate whether USP29 regulates the cGAS related autophagy or senescence."
USP29 affects ACSL5
4 | 9 11
USP29 binds ACSL5.
4 | 4 11
4 | 3 11

sparser
"Thus, targeting USP29-ACSL5 may constitute an effective therapeutic strategy for MASLD."

No evidence text available

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"Moreover, USP29 directly interacted with ACSL5 and stabilized ACSL5 via K48-linked deubiquitination."

sparser
"Furthermore, we found that USP29 directly interacted with ACSL5 and upregulated ACSL5 expression."

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"First, the interaction between USP29 and ACSL5 was further confirmed by co-IP (Fig. 7A, B)."

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"Additionally, a GST pull-down assay revealed a direct interaction between USP29 and ACSL5 (Fig. 7C, D)."

sparser
"USP29 interacts with ACSL5 and upregulates ACSL5 expression."
USP29 binds ACSL5 and K48. 1 / 1
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"USP29 directly interacts with ACSL5 and stabilizes ACSL5 through the suppression of K48-linked ubiquitination.."
USP29 increases the amount of ACSL5.
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USP29 increases the amount of ACSL5. 5 / 5
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"Furthermore, we found that USP29 directly interacted with ACSL5 and upregulated ACSL5 expression."

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"In contrast, USP29 deficiency significantly reduced ACSL5 protein levels in both PAOA-treated hepatocytes and the livers of HFHC diet-fed mice (Fig. 5E, F)."

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"Specifically, they found that USP29 expression is decreased in both human and mouse livers with MASLD, paralleling a reduction in ACSL5 protein levels."

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"USP29 interacts with ACSL5 and upregulates ACSL5 expression."

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"Taken together, these results suggest that USP29 markedly upregulates ACSL5 expression."
USP29 affects MAP3K7
8 | 9 4
USP29 binds MAP3K7.
8 | 2 4
8 | 2 4

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"Moreover, glutathione s-transferase (GST) precipitation assay further demonstrated a direct interaction between USP29 and TAK1 (Figure 7B)."

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"Further results demonstrated that USP29 directly bound and interacted with TAK1."

No evidence text available

sparser
"Specifically, in the context of cardiac hypertrophy, USP29 directly interacts with TAK1 through its binding region (aa 284 to 922), deubiquitinating TAK1 in its K63‐linked polyubiquitination chain within the region (aa 1 to 306) of TAK1 itself."

sparser
"USP29 directly interacted with TAK1, inhibiting its ubiquitination and phosphorylation."

sparser
"ELAVL1 silencing inhibits M1 microglial polarization via the USP29-TAK1 axis, promotes neuronal differentiation of NSCs, and facilitates SCI repair."

sparser
"To further validate the regulatory effect of ELAVL1 on the USP29-TAK1 axis, HMC3 cells were co-infected with shELAVL1 and shUSP29 lentiviruses, followed by LPS-induced polarization (Fig. xref )."
USP29 deubiquitinates MAP3K7.
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USP29 deubiquitinates MAP3K7. 3 / 3
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"Mechanistically, USP29 reduces the sterile inflammation and hepatocellular damage by deubiquitinating TAK1 and preventing its phosphorylation, thereby suppressing the activation of its downstream JNK/p38 pathways."

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"As we expected, USP29 significantly reduced the level of ubiquitination of the TAK1 level (Figure 7E)."

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"As we expected, USP29 significantly decreased the level of ubiquitination of TAK1 after interacting with TAK1."
USP29 inhibits MAP3K7.
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USP29 inhibits MAP3K7. 2 / 2
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"We found that USP29 deregulated the polyubiquitinated state of the k63 TAK1 linkage through deubiquitination, thereby inhibiting TAK1 autophosphorylation and suppressing the activation of the downstream JNK/p38 pathway."

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"For example, USP29 attenuates hepatic ischemia-reperfusion injury by preventing the activation of TAK1 through K63-linked polyubiquitination [21]."
USP29 ubiquitinates MAP3K7.
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USP29 ubiquitinates MAP3K7. 1 / 1
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"Mechanistically, USP29 reduces the sterile inflammation and hepatocellular damage by deubiquitinating TAK1 and preventing its phosphorylation, thereby suppressing the activation of its downstream JNK/p38 pathways."
USP29 decreases the amount of MAP3K7.
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USP29 decreases the amount of MAP3K7. 1 / 1
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"As we expected, USP29 significantly reduced the level of ubiquitination of the TAK1 level (Figure 7E)."
CDK1 affects USP29
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CDK1 phosphorylates USP29.
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CDK1 phosphorylates USP29. 10 / 10
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sparser
"Regarding the fact that both USP29 and CDK1 increase the stability of TWIST1, and CDK1 phosphorylates USP29, We hypothesized that the regulation of TWIST1 by CDK1 might be mediated through the activation of USP29 in TNBC."

sparser
"As shown in Figure  xref , active CDK1 phosphorylated GST‐fused USP29 WT, was largely abolished by the 3A mutation."

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"Phosphorylation of USP29 by CDK1 Governs TWIST1 Stability and Oncogenic Functions."

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"13 We next investigated whether CDK1 could phosphorylate USP29 and affect its tumor‐promoting function in TNBC."

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"Collectively, these results suggest that CDK1 directly interacts with and phosphorylates USP29 other than TWIST1.2.5 CDK1 Regulates TWIST1 Protein Stability, CSC Self-Renewal, and Cellular Sensitivity to Chemotherapy."

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"Regarding the fact that both USP29 and CDK1 increase the stability of TWIST1, and CDK1 phosphorylates USP29, We hypothesized that the regulation of TWIST1 by CDK1 might be mediated through the activation of USP29 in TNBC."

sparser
"Regarding the fact that CDK1 phosphorylates USP29 and USP29 stabilizes TWIST1, we hypothesized that CDK1 might also affect TWIST1 stability and function in TNBC."

sparser
"CDK1 Binds and Phosphorylates USP29."

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"To validate that CDK1 directly phosphorylates USP29, GST‐fused USP29 WT or the 3A mutant were incubated with active CDK1 and in vitro kinase assay was performed."

sparser
"The overexpression of CDK1 has been detected in several types of human cancers and contributes to deregulated cell cycle, DNA damage response, protein synthesis, and other cell cycle independent functions. [ xref ] We next investigated whether CDK1 could phosphorylate USP29 and affect its tumor‐promoting function in TNBC."
CDK1 binds USP29.
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| 3 6

sparser
"In conclusion, our study identifies the previously unknown oncogenic role of CDK1USP29 axis in regulating the stability of TWIST1 and demonstrates their clinical relevance as therapeutic targets in TNBC."

sparser
"In addition, purified GST‐USP29 but not GST could interact with His‐CDK1 in vitro, indicating the direct interaction between USP29 and CDK1 (Figure  xref )."

sparser
"CDK1 Binds and Phosphorylates USP29."

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"In this study, we reveal several unexpected tumor‐promoting functions and the clinical significance of the CDK1USP29 axis through stabilizing TWIST1 and provide preclinical evidence demonstrating that targeting CDK1USP29 axis is an appealing therapeutic strategy to conquer chemo‐resistance and metastasis in TNBC (Figure  xref )."

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"Together, these results uncover a previously unrecognized function of USP29 in tumor progression of TNBC through stabilizing TWIST1.2.4 CDK1 Binds and Phosphorylates USP29."

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"Collectively, these results suggest that CDK1 directly interacts with and phosphorylates USP29 other than TWIST1.2.5 CDK1 Regulates TWIST1 Protein Stability, CSC Self-Renewal, and Cellular Sensitivity to Chemotherapy."

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"In addition, purified GST‐USP29 but not GST could interact with His‐CDK1 in vitro, indicating the direct interaction between USP29 and CDK1 (Figure 4D)."

sparser
"Importantly, we provide preclinical evidence demonstrating that targeting CDK1USP29 axis might be effective to destabilize TWIST1 which decreases the CSCs population, tumor metastasis, and chemo‐resistance of TNBC."

sparser
"Taken together, the findings reveal a previously unrecognized tumor‐promoting function and clinical significance of the CDK1USP29 axis through stabilizing TWIST1 and provide the preclinical evidence that targeting this axis is an appealing therapeutic strategy to conquer chemo‐resistance and metastasis in TNBC."
CDK1 binds USP29 and Thr578. 1 / 1
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"First, we identify that CDK1 binds and phosphorylates USP29 at Ser575, Thr578, and Ser672, while the phosphorylation deficient 3A mutation almost abolishes the phosphorylation of USP29 (Figure 4 and Figure S4, Supporting Information)."
CDK1 activates USP29.
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CDK1 activates USP29. 1 / 1
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"More importantly, the genetic ablation or the pharmacological inhibition of CDK1 could deactivate USP29 and subsequently destabilize TWIST1, thereby largely mitigating EMT, CSC self‐renewal, and metastasis, and sensitizing TNBC cells to chemotherapy."
USP29 is modified
1 | 1 18
USP29 is phosphorylated.
1 | 15
USP29 is phosphorylated. 10 / 12
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sparser
"Phosphorylation could regulate protein‐protein interactions or the enzymatic activity of deubiquitinases. [ xref ] We next investigated whether the effect of CDK1 on TWIST1 and malignant processes in TNBC is mediated by the phosphorylation of USP29."

sparser
"These results indicate that CDK1‐mediated phosphorylation of USP29 is indispensable for its enzymatic activity toward TWIST1 as well as the TWIST1‐dependent malignant phenotypes in TNBC."

sparser
"Furthermore, CDK1‐mediated phosphorylation of USP29 governs TWIST1 stability and oncogenic functions."

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"Mechanistically, CDK1‐mediated phosphorylation of USP29 is essential for its deubiquitinase activity toward TWIST1 and TWIST1 driven‐malignant phenotypes in TNBC, which could be markedly mitigated by the genetic ablation or pharmacological inhibition of CDK1."

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"CDK1, a USP29 activator, facilitates this process through USP29 phosphorylation, enhancing the TWIST1-driven malignant phenotype [ xref ]."

sparser
"CDK1 Binds and Phosphorylates USP29."

sparser
"First, we identify that CDK1 binds and phosphorylates USP29 at Ser575, Thr578, and Ser672, while the phosphorylation deficient 3A mutation almost abolishes the phosphorylation of USP29 (Figure  xref and Figure xref , Supporting Information)."

sparser
"These results dictate that CDK1‐mediated phosphorylation of USP29 is crucial for its deubiquitinase activity toward TWIST1."

sparser
"We next examined the function of CDK1‐mediated phosphorylation of USP29 on TWIST1‐drivened malignant processes."

sparser
"As shown in Figure  xref , Figure xref (Supporting Information), the phosphorylation of USP29 was detected in both MDA‐MB‐231 and BT549 cells by using phospho‐CDK substrate antibody and the genetic ablation or the pharmacological inhibition of CDK1 by RO‐3306 could significantly reduce this phosphorylation event (Figure  xref , Figure xref , Supporting Information)."
USP29 is phosphorylated on T578. 1 / 1
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sparser
"First, we identify that CDK1 binds and phosphorylates USP29 at Ser575, Thr578, and Ser672, while the phosphorylation deficient 3A mutation almost abolishes the phosphorylation of USP29 (Figure  xref and Figure xref , Supporting Information)."
USP29 is phosphorylated on S672. 1 / 1
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sparser
"First, we identify that CDK1 binds and phosphorylates USP29 at Ser575, Thr578, and Ser672, while the phosphorylation deficient 3A mutation almost abolishes the phosphorylation of USP29 (Figure  xref and Figure xref , Supporting Information)."
USP29 is phosphorylated on S585. 1 / 1
1 |

No evidence text available
USP29 is phosphorylated on S575. 1 / 1
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sparser
"First, we identify that CDK1 binds and phosphorylates USP29 at Ser575, Thr578, and Ser672, while the phosphorylation deficient 3A mutation almost abolishes the phosphorylation of USP29 (Figure  xref and Figure xref , Supporting Information)."
USP29 is ubiquitinated.
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USP29 is ubiquitinated. 2 / 2
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sparser
"As shown in Fig. 7 B and Fig. S7B, we noted that the USP29 enzymatic mutant (USP29-mut) underwent ubiquitination."

sparser
"Accordingly, we found that USP29 1–280 underwent ubiquitination, which was eliminated by USP29 ( Fig. 8 F and Fig. S8F)."
USP29 is sumoylated.
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USP29 is sumoylated. 1 / 1
| 1

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"In addition, we found that TRIM38 and USP29 interacts with cGAS independently, suggesting that TRIM38 mediated sumoylation and USP29 mediated deubiquitination of cGAS cooperatively regulate the stability of cGAS."
USP29 is methylated.
| 1
USP29 is methylated. 1 / 1
| 1

sparser
"Additionally, melatonin reduces m 6 A RNA methylation of USP29, contributing to its upregulation ( xref ; xref )."
CGAS affects USP29
| 16 1
USP29 binds cGAS. 10 / 14
| 14

sparser
"Our results suggest that HSV-1 infection upregulated USP29, which might be responsible for increased USP29-cGAS association after HSV-1 infection."

sparser
"Deubiquitinases like USP14 [ xref ], USP27x [ xref ], and USP29 [ xref ] interact with cGAS to remove K48-linked ubiquitin chains, thereby stabilizing the cGAS protein."

sparser
"Domain mapping analysis showed that the ubiquitin carboxyl-terminal hydrolase (UCH) domain of USP29 and the C-terminal domain containing the nucleotidyltransferase (NTase) domain of cGAS were responsible for USP29-cGAS association (Fig.  xref )."

sparser
"Interestingly, we found that TRIM38 constitutively interacts with cGAS but not with USP29, whereas USP29 constitutively interacts with cGAS but not with TRIM38 (Supplementary information, Fig.  xref ), indicating that TRIM38 and USP29 independently interacts with and regulates cGAS."

sparser
"Together, these data suggest that USP29 interacts with cGAS directly and constitutively in resting cells and after HSV-1 infection."

sparser
"Because USP29 interacted with cGAS and was upregulated by HSV-1 infection, we investigated its role in regulating HSV-1- or cytoplasmic DNA-triggered innate immune signaling."

sparser
"Previous studies have demonstrated that accumulation of dsDNA in the cytoplasm induces micronuclei-localization of cGAS. xref , xref , xref In our study, we found that a portion of USP29-cGAS associating spots were localized at perinuclear compartments after HSV-1 infection."

sparser
"Although the nature of such subcellular compartments is unclear, the localization of cGAS might also play a role for increased USP29-cGAS association after HSV-1 infection."

sparser
"Mechanistically, USP29 constitutively interacts with cGAS, deconjugates K48-linked polyubiquitin chains from cGAS and stabilizes cGAS in uninfected cells or after HSV-1 infection."

sparser
"It has been proposed that three pools of cGAS exist in uninfected cells: unmodified, Lys217-sumoylated and constitutively Lys271-ubiquitinated cGAS. xref Sumoylation at Lys217 antagonizes K48-linked ubiquitination of Lys271 of cGAS, thereby inhibiting its proteasomal degradation. xref In our study, we found that USP29 constitutively interacted with cGAS and USP29 deficiency substantially potentiated basal K48-linked ubiquitination of cGAS and downregulated cGAS protein level without affecting its mRNA expression in uninfected cells, indicating that USP29 constitutively removes K48-linked ubiquitination of cGAS to maintain a relatively high level of cGAS under steady conditions."
TRIM38 binds USP29 and cGAS. 1 / 1
| 1

sparser
"In addition, we found that TRIM38 and USP29 interacts with cGAS independently, suggesting that TRIM38-mediated sumoylation and USP29-mediated deubiquitination of cGAS cooperatively regulate the stability of cGAS."
USP25 binds USP29 and cGAS. 1 / 1
| 1

sparser
"To test this hypothesis, we conducted an unbiased screening of cGAS-interacting DUBs by cotransfection and immunoprecipitation assays as previous established, xref – xref and found that USP25 and USP29 interacted with cGAS in such a system (Fig.  xref )."
USP29 binds cGAS. 1 / 1
| 1

isi
"Mechanistically, USP29 constitutively interacts with cGAS, deconjugates K48-linked polyubiquitin chains from cGAS and stabilizes cGAS in uninfected cells or after HSV-1 infection."
USP29 affects KMT5A
2 | 8 7
USP29 binds KMT5A.
2 | 2 7
2 | 2 7

sparser
"SETD8 and USP29 Interact In Vivo."

sparser
"These data demonstrate that SETD8 and USP29 interact in vivo."

reach
"We demonstrate that SETD8 binds to USP29 in vivo, and this interaction is dependent on the catalytic activity of USP29."

sparser
"We demonstrate that SETD8 binds to USP29 in vivo, and this interaction is dependent on the catalytic activity of USP29."

sparser
"Together these data show that SETD8 and the USP29 bind to each other in vivo, and that this interaction depends on the ubiquitin hydrolase activity of USP29."

reach
"With several substrates among proteins functioning in the DNA damage response, USP29 can be considered as one of the central ubiquitin hydrolases contributing to the maintenance of genome integrity.Our results show that the decreased levels of SETD8 in cells depleted of USP29 do not have an indirect effect on cell cycle progression and, moreover, demonstrate an interaction between USP29 and SETD8 in vivo."

sparser
"Our results show that the decreased levels of SETD8 in cells depleted of USP29 do not have an indirect effect on cell cycle progression and, moreover, demonstrate an interaction between USP29 and SETD8 in vivo."

No evidence text available

No evidence text available

sparser
"Interestingly, a catalytic inactive version of USP29 did not coimmunoprecipitate with SETD8, indicating that the ubiquitin hydrolase activity is required for the interaction of SETD8 with USP29."
USP29 deubiquitinates KMT5A.
| 5
USP29 deubiquitinates KMT5A. 5 / 5
| 5

reach
"USP29 Deubiquitinates SETD8 and Regulates DNA Damage-Induced H4K20 Monomethylation and 53BP1 Focus Formation."

reach
"Wildtype USP29 can deubiquitinate SETD8 in vivo, indicating that USP29 directly regulates SETD8 protein levels."

reach
"Furthermore, USP29 deubiquitinates SETD8 in vivo and controls the DNA damage-induced monomethylation of H4K20 and focus formation of 53BP1."

reach
"USP29 Deubiquitinates SETD8 In Vivo."

reach
"Indeed, overexpression of wildtype USP29 diminished polyubiquitination of SETD8 in vivo, whereas the polyubiquitinated SETD8 was less affected by a co-expression of a catalytic inactive version of the ubiquitin hydrolase.We next demonstrated that the depletion of USP29 inhibited the accumulation of 53BP1 into foci after treating the cells with IR."
USP29 increases the amount of KMT5A.
| 1
USP29 increases the amount of KMT5A. 1 / 1
| 1

reach
"Wildtype USP29 can deubiquitinate SETD8 in vivo, indicating that USP29 directly regulates SETD8 protein levels."
| 1 13
| 1 10

reach
"Liu et al. reported that USP29 expression was elevated upon oxidative stress and then promoted cell apoptosis via directly deubiquitinating p53 [25]."

eidos
"Recently , a study reported that USP29 depletion decreased apoptosis by regulating the Snail protein [ 13 ] ."

reach
"We also demonstrated that USP29 depletion hampers the colony formation and increases apoptosis of HCT116 cells."

reach
"Neuron-specific USP29 knockout significantly diminished, whereas USP29 overexpression aggravated cerebral I/R-induced oxidative stress, apoptosis, and neurological dysfunction in mice."

reach
"In addition, OGD/R-induced oxidative stress and neuronal apoptosis were also attenuated by USP29 silence but exacerbated by USP29 overexpression in vitro."

reach
"Collectively, these findings imply that USP29 potentiates I/R-induced oxidative damage and neuronal apoptosis in vitro."

reach
"Neuron-specific USP29 knockout significantly diminishes, whereas USP29 overexpression aggravates cerebral I/R-induced oxidative stress, apoptosis, and neurological dysfunction in vivo and in vitro."

reach
"Based on the above results, we conclude that USP29 upregulation during cerebral I/R injury potentiates oxidative stress, apoptosis, and neurological dysfunction in mice."

reach
"Generally, our findings for the first time identify that USP29 upregulation during cerebral I/R may contribute to oxidative stress, neuronal apoptosis, and the progression of cerebral I/R injury and that inhibition of USP29 may help to develop novel therapeutic strategies to treat cerebral I/R injury.Multiple mechanisms are implicated in the pathogenesis of cerebral I/R injury, including oxidative stress and apoptosis."

reach
"Our findings for the first time identify that USP29 upregulation during cerebral I/R may contribute to oxidative stress, neuronal apoptosis, and the progression of cerebral I/R injury and that inhibition of USP29 may help to develop novel therapeutic strategies to treat cerebral I/R injury."
| 3

reach
"The above results suggest that USP29 deficiency promotes local inflammation and hepatocyte apoptosis to exacerbate hepatic I/R injury."

reach
"USP29 overexpression inhibits hepatic injury, inflammatory response, and apoptosis."

reach
"Indeed, our findings, along with previous reports that depletion of USP29 caused cell cycle arrest and apoptosis in cervical cancer [25], colorectal cancer [38], and osteosarcoma cell [41]."
USP29 affects AURKB
3 | 9 2
USP29 binds AURKB.
3 | 2 2
3 | 2 2

sparser
"USP29 interacts with AURKB and removes its K48-linked polyubiquitination."

sparser
"Moreover, co-immunoprecipitation (Co-IP) using MGC-803 and HGC-27 cell lysates validated the specific interaction between endogenous USP29 and AURKB in gastric cancer cells (Fig.  xref D)."

No evidence text available

reach
"In terms of mechanism, USP29 is transcriptionally activated by FUBP1, and the deregulated USP29 interacts with and stabilizes AURKB, forming a FUBP1-USP29-AURKB axis that enables tumor cell proliferation and survival in gastric cancers."

reach
"Moreover, co-immunoprecipitation (Co-IP) using MGC-803 and HGC-27 cell lysates validated the specific interaction between endogenous USP29 and AURKB in gastric cancer cells (Fig. 2D)."

No evidence text available

No evidence text available
USP29 activates AURKB.
| 5
USP29 activates AURKB. 5 / 5
| 5

reach
"On the other hand, depletion of endogenous USP29 using sgRNAs or shRNAs significantly decreased the AURKB protein abundance, but showed minimal effects on AURKB mRNA level (Fig. 3B and C and Supplementary Fig. 3A), indicating that USP29 regulates AURKB expression at the protein level."

reach
"Here we found that USP29 rescued CDH1-mediated decrease of AURKB and proteasome inhibitor MG132 treatment also rescued the AURKB downregulation induced by USP29 depletion (Fig. 3D and E)."

reach
"Consistently, depletion of endogenous USP29 in MGC-803 cells using sgRNAs significantly accelerated degradation of endogenous AURKB (Fig. 3G)."

reach
"USP29 activation mediated by FUBP1 promotes AURKB stability and oncogenic functions in gastric cancer."

reach
"USP29 enhances AURKB protein stability."
USP29 inhibits AURKB.
| 2
USP29 inhibits AURKB. 2 / 2
| 2

reach
"Consistently, USP29 deletion markedly suppressed the AURKB protein abundance in all three cell lines (Supplementary Fig. 3C), arguing that this regulation exists in different types of tumors."

reach
"Here we found that USP29 rescued CDH1-mediated decrease of AURKB and proteasome inhibitor MG132 treatment also rescued the AURKB downregulation induced by USP29 depletion (Fig. 3D and E)."
TWIST1 affects USP29
| 4 10
| 4 10

sparser
"As shown in Figure  xref , only the full‐length (FL) and M4 mutant (687‐922aa) region of USP29, but not other mutants, could interact with TWIST1."

sparser
"We next confirmed the endogenous TWIST1USP29 interaction by co‐immunoprecipitation assay."

sparser
"We first examined whether CDK1 regulated the interaction between USP29 with TWIST1."

sparser
"All three sites are outside of the domain (687‐922aa) that mediates the interaction between USP29 and TWIST1."

sparser
"The direct interaction of USP29 and TWIST1 prompted us to examine a potential role of USP29 in the regulation of TWIST1 stability and function."

sparser
"As shown in Figure xref (Supporting Information), the treatment of RO‐3306 in MDA‐MB‐231 cells did not affect the USP29TWIST1 interaction."

sparser
"USP29 directly binds, deubiquitinates, and stabilizes TWIST1, which in turn promotes TWIST1‐driven tumor progression in TNBC."

reach
"USP29 directly binds, deubiquitinates, and stabilizes TWIST1, which in turn promotes TWIST1‐driven tumor progression in TNBC."

reach
"The direct interaction of USP29 and TWIST1 prompted us to examine a potential role of USP29 in the regulation of TWIST1 stability and function."

reach
"In addition, purified GST‐USP29 but not GST pulled down His‐TWIST1 in vitro, indicating the direct interaction between USP29 and TWIST1 (Figure 1D)."
MAP3K7 affects USP29
8 | 2 4
8 | 2 4

No evidence text available

No evidence text available

No evidence text available

reach
"Moreover, glutathione s-transferase (GST) precipitation assay further demonstrated a direct interaction between USP29 and TAK1 (Figure 7B)."

reach
"Further results demonstrated that USP29 directly bound and interacted with TAK1."

No evidence text available

sparser
"Specifically, in the context of cardiac hypertrophy, USP29 directly interacts with TAK1 through its binding region (aa 284 to 922), deubiquitinating TAK1 in its K63‐linked polyubiquitination chain within the region (aa 1 to 306) of TAK1 itself."

sparser
"USP29 directly interacted with TAK1, inhibiting its ubiquitination and phosphorylation."

sparser
"ELAVL1 silencing inhibits M1 microglial polarization via the USP29-TAK1 axis, promotes neuronal differentiation of NSCs, and facilitates SCI repair."

sparser
"To further validate the regulatory effect of ELAVL1 on the USP29-TAK1 axis, HMC3 cells were co-infected with shELAVL1 and shUSP29 lentiviruses, followed by LPS-induced polarization (Fig. xref )."
CGAS affects USP29
2 | 12
CGAS binds USP29.
2 | 11
2 | 8

reach
"To test this hypothesis, we conducted an unbiased screening of cGAS interacting DUBs by cotransfection and immunoprecipitation assays as previous established, XREF_BIBR - XREF_BIBR and found that USP25 and USP29 interacted with cGAS in such a system."

reach
"In addition, USP29 selectively interacted with cGAS but not with other molecules including MITA, RIG-I, MAVS, TBK1 or IRF3 (Supplementary information, Fig.S2d)."

reach
"Results from endogenous immunoprecipitation assays suggested that USP29 constitutively interacted with cGAS in bone marrow derived macrophages (BMDMs), bone marrow derived dendritic cells (BMDCs), mouse embryonic fibroblasts (MEFs), mouse lung fibroblasts (MLFs) or human monocytic THP-1 cells and their association was potentiated after HSV-1 infection."

reach
"Interestingly, we found that TRIM38 constitutively interacts with cGAS but not with USP29, whereas USP29 constitutively interacts with cGAS but not with TRIM38 (Supplementary information, Fig.S2h), indicating that TRIM38 and USP29 independently interacts with and regulates cGAS."

reach
"Together, these data suggest that USP29 interacts with cGAS directly and constitutively in resting cells and after HSV-1 infection."

reach
"Because USP29 interacted with cGAS and was upregulated by HSV-1 infection, we investigated its role in regulating HSV-1- or cytoplasmic DNA triggered innate immune signaling."

reach
"Because USP29 interacts with cGAS and its enzyme activity is required for regulating antiviral signaling, we investigated whether USP29 eliminated polyubiquitin chains from cGAS."

reach
"In addition, we found that TRIM38 and USP29 interacts with cGAS independently, suggesting that TRIM38 mediated sumoylation and USP29 mediated deubiquitination of cGAS cooperatively regulate the stability of cGAS."

No evidence text available

No evidence text available
USP29 binds CGAS and K48. 3 / 3
| 3

reach
"Mechanistically, USP29 constitutively interacts with cGAS, deconjugates K48 linked polyubiquitin chains from cGAS and stabilizes cGAS in uninfected cells or after HSV-1 infection."

reach
"[208] Recently, a study reported that USP29 interacts with cGAS, hydrolyzes K48 linked polyubiquitin chains on cGAS, and stabilizes cGAS in uninfected cells, or after HSV-1 stimulation."

reach
"USP29 constitutively interacts with cGAS and deconjugates K48 linked polyubiquitin chains from cGAS in the absence or presence of HSV-1 infection, which stabilizes cGAS and promotes cGAS mediated cellular antiviral responses."
CGAS inhibits USP29.
| 1
CGAS inhibits USP29. 1 / 1
| 1

reach
"Interestingly, the levels of cGAS and the expression of Tnf, Il6, Il12p40 and Ifit3 were significantly decreased in Trex1 -/- Usp29 m/m BMDCs compared to Trex1 -/- BMDCs (Supplementary information, Fig.S7d)."
SNAI1 affects USP29
6 | 2 5
6 | 2 5

reach
"We then moved to investigate the potential interaction between USP29 and Snail1."

reach
"These observations imply the necessity of USP29 DUB activity in the regulation of Snail1, but the exact roles of USP29 mutations detected in cancer samples require further clarification.To evaluate the interaction between Snail1 and USP29, we carried out a series of co-immunoprecipitation experiments."

No evidence text available

No evidence text available

sparser
"Cancer cells hijack the USP29-Snail1 axis to facilitate chemoresistance."

sparser
"Considering the pivotal roles that Snail1 plays during the development of resistance to chemotherapies, we posited that the USP29-Snail1 axis might be exploited by cancer cells in their acquisition of chemoresistance."

No evidence text available

sparser
"USP29 interacts with Snail1 and requires catalytic activity to stabilize Snail1."

No evidence text available

No evidence text available
USP29 affects NPPA
| 12
| 12

sparser
"Several medicines determined by the 1 H qNMR method were published in USP29-NF24, including assays of amyl nitrite and its inhalant and the isomer content of orphenadrine citrate [11] ."

sparser
"Both the United States pharmacopoeia (USP29-NF24) and European pharmacopeia (EP, 01/2008:0854) reversed-phase high-performance liquid chromatography (RP-HPLC) methods were used to analyze several insulin drug products purchased from local pharmacies."

sparser
"To demonstrate proper verification of the insulin content in drug products, a quantitative analysis was performed on products containing recombinant hI, porcine insulin, and one hI analog, using the corresponding insulin-type-specific USP (USP29-NF24) and EP monographs appropriately validated for quantitation."

sparser
"In an initial screening assessment, all insulin products were tested with the chromatographic procedure described in the RP-HPLC identification method of the USP monograph (USP29-NF24), “Insulin.” Although this chromatographic procedure is only intended for use with human and porcine insulin products, we used this as a rapid preliminary assessment to screen qualitatively for potentially abnormal insulin traces in all products."

sparser
"To properly verify that insulin content in marketed drug products met label claims, a quantitative analysis was performed on a subset of purchased products using the RP-HPLC method from the corresponding type-specific USP (USP29-NF24) and EP (01/2008:0854) monographs."

sparser
"All VD3-loaded tablets prepared by direct compression showed acceptable friability values (less than 1%), which were according to the USP friability compendial guidelines (USP29NF24) in the corresponding monograph > TABLET > FRIABILITY."

sparser
"The specification limits for a 2208 grade are 19–24% (w/w) for MeO and 4–12% (w/w) for HPO ( Pharmacopoeia, 2006; USP29-NF24, 2003 )."

sparser
"The solution properties of HPMC can be altered by changing the molar mass and/or the degree of substitution and thus in the case of pharmaceutically approved HPMC, there are different substituent and [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"This to some extent be explained by the broad specifications for commercial grades, allowing rather large variability in parameters such as the viscosity and the degree of substitution ( Pharmacopoeia[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In addition, the content of paracetamol was also quantified using assay method (USP29-NF24) to ensure that the blending of small (excipient) and large (paracetamol) powder components resulted in homog[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP29 affects Neoplasms
| 1 10
USP29 activates Neoplasms.
| 1 8
| 1 7

reach
"Consistently, USP29 deletion markedly suppressed the AURKB protein abundance in all three cell lines (Supplementary Fig. 3C), arguing that this regulation exists in different types of tumors."

eidos
"In conclusion , our study shows that elevated expression of USP29 promotes malignancy in CRC , suggesting that USP29 could be a promising target for colon cancer therapy ."

reach
"Taken together, these results suggest that USP29 is a bona fide DUB targeting TWIST1 protein for deubiquitination and stabilization.2.3 USP29 Promotes Tumor Progression of TNBC through Stabilizing TWIST1."

reach
"Consisting with the findings obtained in vitro, USP29 knockdown significantly suppressed tumor growth in vivo (Fig. 1F and G)."

reach
"Such tumor-promoting effects of USP29 were experimentally validated through a series of in vitro and in vivo phenotypic assays, with several lines of evidence in support of the notion that USP29 expression positively correlated with the enhanced stemness of lung adenocarcinoma cells.It is intriguing that certain labile transcription factors can be stabilized by diverse DUBs, with p53 showing a leading example."

reach
"Our results demonstrate USP29 promotes tumor progression in TNBC through TWIST1."

reach
"In our previous study, we revealed that USP29 promoted tumor cell aerobic glycolysis and glutamine anaplerosis by deubiquitinating and stabilizing the oncogenic MYC family and HIF1α, and systemic knockout of USP29 significantly suppressed tumor progression and prolonged the survival of tumor-bearing mice."

reach
"Systemic knockout of Usp29 depleted MYC and HIF1α in MYC-driven neuroblastoma and B cell lymphoma, inhibited critical metabolic targets and significantly prolonged survival of tumor-bearing mice."
USP29 bound to TWIST1 activates Neoplasms. 1 / 1
| 1

reach
"USP29 directly binds, deubiquitinates, and stabilizes TWIST1, which in turn promotes TWIST1‐driven tumor progression in TNBC."
USP29 inhibits Neoplasms.
| 2
| 2

reach
"IHC analysis of Ki-67 and cleaved caspase-3 demonstrated strong inhibition of cell proliferation and massive intratumoral cell death in USP29-depleted tumors (Fig. 1H and I)."

reach
"In our previous study, we revealed that USP29 promoted tumor cell aerobic glycolysis and glutamine anaplerosis by deubiquitinating and stabilizing the oncogenic MYC family and HIF1α, and systemic knockout of USP29 significantly suppressed tumor progression and prolonged the survival of tumor-bearing mice."
USP29 affects MYC
1 | 6 4
USP29 binds MYC.
1 | 4
1 | 4

sparser
"To validate the mass spectrometry data, we performed reciprocal immunoprecipitation with co-expression of Flag-AURKB and Myc-USP29 in 293T cells and confirmed associations between these both proteins (Fig.  xref C)."

sparser
"HEK 293 T cells were transfected with both Myc-USP29 (wild-type or with C223S mutation), HA-Ub, and Flag-FTO to confirm that USP7 controls the ubiquitination of FTO."

No evidence text available

sparser
"To examine which kind of polyubiquitin chains USP29 removes from AURKB, we co-transfected 293T cells with Flag-AURKB, Myc-USP29 and HA-tagged ubiquitin mutants retaining a single lysine residue, and uncovered that USP29 specifically deconjugated the K48-linked polyubiquitin chains (Fig.  xref G), a well-established destruction signal to trigger 26 S proteasome-mediated proteolysis [ xref ]."

sparser
"To further explore this notion, Flag-AURKB, Myc-USP29, HA-tagged ubiquitin (WT) or K48R mutant were co-transfected into 293T cells, and the results showed that USP29 failed to catalyze removal of K48R-linked polyubiquitin chains from AURKB (Fig.  xref H)."
USP29 activates MYC.
| 5
USP29 activates MYC. 5 / 5
| 5

reach
"Our previous study showed that USP29 positively regulates the MYC downstream pathway."

reach
"These results indicated that USP29 may promote nucleotide biosynthesis by regulating the MYC and E2F downstream pathways."

reach
"Knockout of USP29 depletes MYC and markedly prolongs survival of lymphoma xenografts [ 45 ]."

reach
"Analysis of RNA sequencing data in SK-N-BE2 cells demonstrated that USP29 promoted the gene expression of metabolic enzymes involved in nucleotide anabolism, probably by regulating MYC and E2F downstream pathways."

reach
"Conversely, USP37 and USP29 promote tumorigenesis by stabilizing c-Myc through deubiquitination [22, 23]."
USP29 increases the amount of MYC.
| 1
USP29 increases the amount of MYC. 1 / 1
| 1

reach
"The USP29 peptidase promotes elevated MYC levels via the stabilization of the MYC protein."
USP29 affects CDK1
| 5 6
USP29 binds CDK1.
| 4 6
| 3 6

sparser
"In conclusion, our study identifies the previously unknown oncogenic role of CDK1USP29 axis in regulating the stability of TWIST1 and demonstrates their clinical relevance as therapeutic targets in TNBC."

sparser
"In addition, purified GST‐USP29 but not GST could interact with His‐CDK1 in vitro, indicating the direct interaction between USP29 and CDK1 (Figure  xref )."

sparser
"CDK1 Binds and Phosphorylates USP29."

sparser
"In this study, we reveal several unexpected tumor‐promoting functions and the clinical significance of the CDK1USP29 axis through stabilizing TWIST1 and provide preclinical evidence demonstrating that targeting CDK1USP29 axis is an appealing therapeutic strategy to conquer chemo‐resistance and metastasis in TNBC (Figure  xref )."

reach
"Together, these results uncover a previously unrecognized function of USP29 in tumor progression of TNBC through stabilizing TWIST1.2.4 CDK1 Binds and Phosphorylates USP29."

reach
"Collectively, these results suggest that CDK1 directly interacts with and phosphorylates USP29 other than TWIST1.2.5 CDK1 Regulates TWIST1 Protein Stability, CSC Self-Renewal, and Cellular Sensitivity to Chemotherapy."

reach
"In addition, purified GST‐USP29 but not GST could interact with His‐CDK1 in vitro, indicating the direct interaction between USP29 and CDK1 (Figure 4D)."

sparser
"Importantly, we provide preclinical evidence demonstrating that targeting CDK1USP29 axis might be effective to destabilize TWIST1 which decreases the CSCs population, tumor metastasis, and chemo‐resistance of TNBC."

sparser
"Taken together, the findings reveal a previously unrecognized tumor‐promoting function and clinical significance of the CDK1USP29 axis through stabilizing TWIST1 and provide the preclinical evidence that targeting this axis is an appealing therapeutic strategy to conquer chemo‐resistance and metastasis in TNBC."
CDK1 binds USP29 and Thr578. 1 / 1
| 1

reach
"First, we identify that CDK1 binds and phosphorylates USP29 at Ser575, Thr578, and Ser672, while the phosphorylation deficient 3A mutation almost abolishes the phosphorylation of USP29 (Figure 4 and Figure S4, Supporting Information)."
USP29 activates CDK1.
| 1
USP29 activates CDK1. 1 / 1
| 1

reach
"In addition, the overexpression of USP29 dramatically decreased the ubiquitination level of TWIST1, which was markedly blocked by the treatment of RO‐3306 or the depletion of CDK1 (Figure 6B,C)."
KMT5A affects USP29
2 | 2 7
2 | 2 7

sparser
"SETD8 and USP29 Interact In Vivo."

sparser
"These data demonstrate that SETD8 and USP29 interact in vivo."

reach
"We demonstrate that SETD8 binds to USP29 in vivo, and this interaction is dependent on the catalytic activity of USP29."

sparser
"We demonstrate that SETD8 binds to USP29 in vivo, and this interaction is dependent on the catalytic activity of USP29."

sparser
"Together these data show that SETD8 and the USP29 bind to each other in vivo, and that this interaction depends on the ubiquitin hydrolase activity of USP29."

reach
"With several substrates among proteins functioning in the DNA damage response, USP29 can be considered as one of the central ubiquitin hydrolases contributing to the maintenance of genome integrity.Our results show that the decreased levels of SETD8 in cells depleted of USP29 do not have an indirect effect on cell cycle progression and, moreover, demonstrate an interaction between USP29 and SETD8 in vivo."

sparser
"Our results show that the decreased levels of SETD8 in cells depleted of USP29 do not have an indirect effect on cell cycle progression and, moreover, demonstrate an interaction between USP29 and SETD8 in vivo."

No evidence text available

No evidence text available

sparser
"Interestingly, a catalytic inactive version of USP29 did not coimmunoprecipitate with SETD8, indicating that the ubiquitin hydrolase activity is required for the interaction of SETD8 with USP29."
USP29 affects Flag
| 10
| 10

sparser
"1) HEK293T cells transfected FlagUSP29 and HA‐TWIST1 as indicated were lysed in NETN buffer and were incubated 4 h with Flag‐beads or HA‐beads for 4 °C."

sparser
"To define the precise region(s) in USP29 for this interaction, we constructed various Flag-USP29 mutants, and expressed a full-length HA-tagged AURKB in combination with respective Flag-USP29 fragments in 293T cells."

sparser
"To assess the potential role of USP29 in regulating AURKB ubiquitination, we co-expressed HA-AURKB, Myc-Ub, and Flag-USP29 in 293T cells, and revealed thatUSP29 markedly impaired the polyubiquitination of AURKB, which requires the DUB activity of USP29, as the catalytic-inactive C294S (substitution of cysteine 294 to serine) mutant that was reported to display a similar substrate-binding capacity [ xref ], failed to reduce AURKB polyubiquitination (Fig.  xref F)."

sparser
"IP analysis of TAK1 ubiquitination levels demonstrated that Flag-USP29 transfection significantly reduced TAK1 ubiquitination compared to the Myc-TAK1 and HA-Ub co-transfection group (Fig. xref )."

sparser
"We examined the cell activity after the H/R challenge with a CCK-8 assay and found that the cell viability of the Flag-USP29 group was significantly higher than that of control cells ( xref ), which indicates that USP29 overexpression enhanced the cells’ tolerance to the H/R challenge."

sparser
"To test this hypothesis, we ectopically expressed Flag-USP29 in gastric cancer cells and revealed that USP29 overexpression increased the AURKB protein levels (Fig.  xref A)."

sparser
"To confirm the influence of USP29 on AURKB protein stability, we transfected 293T cells with HA-AURKB and Flag-USP29, and treated the cells with CHX, a widely used inhibitor for protein translation."

sparser
"To identify the binding partners of USP29, we infected 293T cells with vector control and Flag-USP29, respectively, and Flag-bound immunoprecipitates from these cells were resolved by SDS-PAGE and visualized by silver staining (Fig.  xref A)."

sparser
"Furthermore, cotransfection experiments conducted in HEK293T cells with Myc‐ubiquitinated and HA‐TAK1 demonstrated that introduction of FlagUSP29 significantly reduced the level of TAK1 polyubiquitination (Figure  xref )."

sparser
"The proteomic data revealed 81 and 39 putative binding proteins (with coverage > 30%) in control and Flag-USP29 immunoprecipitates, respectively (Supplementary Fig.  xref A), and AURKB, a well-established oncogenic driver, was specifically immunoprecipitated by USP29 (Fig.  xref B and Supplementary Fig.  xref B)."
USP29 affects CLSPN
2 1 | 1 4
USP29 binds CLSPN.
2 | 1 1
2 | 1 1

No evidence text available

No evidence text available

reach
"USP29 interacts with Claspin and is able to deubiquitinate it both in vivo and in vitro."

trips
"USP29 interacts with Claspin and is able to deubiquitinate it both in vivo and in vitro."
USP29 activates CLSPN.
| 2
USP29 activates CLSPN. 2 / 3
| 2

reach
"The deubiquitinases USP9X and USP29 promote Claspin stabilization during the S phase, and thus, adequate replication fork progression, by opposing its proteasomal degradation [23,24]."

reach
"A recent report indicates that USP29 promotes CLASPIN (not CHK1) stability, ensuring CHK1 activation in response to UV irradiation."
USP29 increases the amount of CLSPN.
| 1
USP29 increases the amount of CLSPN. 1 / 1
| 1

reach
"In contrast, knockdown of endogenous USP29 (TOV21G cells transfected with sh-USP29) did not reduce the Claspin protein expression (data not shown)."
USP29 deubiquitinates CLSPN.
1 |
USP29 deubiquitinates CLSPN. 1 / 1
1 |

"<span class="match term0">USP29</span> controls the stability of checkpoint adaptor <span class="match term1">Claspin</span> by deubiquitination"
USP29 affects 9b
3 | 7
USP29 increases the amount of 9b.
| 5
USP29 increases the amount of 9b. 5 / 5
| 5

reach
"The results showed that overexpression of ORF9b increased the SARS-CoV-2 trVLP infectivity, whereas USP29 further enhanced the SARS-CoV-2 trVLP infectivity by upregulating the expression of ORF9b (Fig. 7A)."

reach
"As expected, overexpression of USP29 increased the expression of ORF9b, resulting in stronger IFN-β and NF-κB inhibition than ORF9b alone (Fig. 5A and B)."

reach
"Accordingly, USP29 WT but not C294A increased the expression of ORF9b (Fig. S4A-B)."

reach
"In contrast, knockdown of USP29 reduced the expression of ORF9b, thereby releasing IFN-β induction and NF-κB activation (Fig. 5G–H)."

reach
"As expected, USP29 increased ORF9b expression (Fig. 6E)."
USP29 binds 9b.
3 | 1
3 | 1

reach
"We performed forward and reverse IP assays, which showed the interaction between USP29 and ORF9b (Fig. S3A–B)."

No evidence text available

No evidence text available

No evidence text available
USP29 deubiquitinates 9b.
| 1
USP29 deubiquitinates 9b. 1 / 1
| 1

reach
"The results also showed that USP29 WT, but not the C294A mutant, efficiently deubiquitinated ORF9b (Fig. 3B)."
Flag affects USP29
| 10
| 10

sparser
"1) HEK293T cells transfected FlagUSP29 and HA‐TWIST1 as indicated were lysed in NETN buffer and were incubated 4 h with Flag‐beads or HA‐beads for 4 °C."

sparser
"To define the precise region(s) in USP29 for this interaction, we constructed various Flag-USP29 mutants, and expressed a full-length HA-tagged AURKB in combination with respective Flag-USP29 fragments in 293T cells."

sparser
"To assess the potential role of USP29 in regulating AURKB ubiquitination, we co-expressed HA-AURKB, Myc-Ub, and Flag-USP29 in 293T cells, and revealed thatUSP29 markedly impaired the polyubiquitination of AURKB, which requires the DUB activity of USP29, as the catalytic-inactive C294S (substitution of cysteine 294 to serine) mutant that was reported to display a similar substrate-binding capacity [ xref ], failed to reduce AURKB polyubiquitination (Fig.  xref F)."

sparser
"IP analysis of TAK1 ubiquitination levels demonstrated that Flag-USP29 transfection significantly reduced TAK1 ubiquitination compared to the Myc-TAK1 and HA-Ub co-transfection group (Fig. xref )."

sparser
"We examined the cell activity after the H/R challenge with a CCK-8 assay and found that the cell viability of the Flag-USP29 group was significantly higher than that of control cells ( xref ), which indicates that USP29 overexpression enhanced the cells’ tolerance to the H/R challenge."

sparser
"To test this hypothesis, we ectopically expressed Flag-USP29 in gastric cancer cells and revealed that USP29 overexpression increased the AURKB protein levels (Fig.  xref A)."

sparser
"To confirm the influence of USP29 on AURKB protein stability, we transfected 293T cells with HA-AURKB and Flag-USP29, and treated the cells with CHX, a widely used inhibitor for protein translation."

sparser
"To identify the binding partners of USP29, we infected 293T cells with vector control and Flag-USP29, respectively, and Flag-bound immunoprecipitates from these cells were resolved by SDS-PAGE and visualized by silver staining (Fig.  xref A)."

sparser
"Furthermore, cotransfection experiments conducted in HEK293T cells with Myc‐ubiquitinated and HA‐TAK1 demonstrated that introduction of FlagUSP29 significantly reduced the level of TAK1 polyubiquitination (Figure  xref )."

sparser
"The proteomic data revealed 81 and 39 putative binding proteins (with coverage > 30%) in control and Flag-USP29 immunoprecipitates, respectively (Supplementary Fig.  xref A), and AURKB, a well-established oncogenic driver, was specifically immunoprecipitated by USP29 (Fig.  xref B and Supplementary Fig.  xref B)."

reach
"In conclusion, these results indicate that USP29 knockout accelerates hepatic steatosis, inflammation and fibrosis induced by a HFHC diet."

reach
"Intriguingly, USP29 overexpression obviously attenuated hepatic steatosis, inflammation, collagen deposition and liver injury (Supplementary Fig. 4H–K)."

reach
"Thus, our results suggest that USP29 attenuates hepatic I/R injury by inhibiting the inflammatory response and apoptosis.We performed an RNA-sequencing (RNA-Seq) analysis of liver tissues from USP29 knockout mice and controls with I/R injury after 3h, and the results of the KEGG signaling pathway enrichment analysis showed that the MAPK signaling pathway was the most affected after USP29 knockout."

reach
"Hepatic steatosis, inflammation and fibrosis were significantly exacerbated by USP29 deletion and relieved by USP29 overexpression."

reach
"The above results suggest that USP29 deficiency promotes local inflammation and hepatocyte apoptosis to exacerbate hepatic I/R injury."

reach
"USP29 overexpression inhibits hepatic injury, inflammatory response, and apoptosis."

reach
"USP29 knockout accelerates hepatic steatosis, inflammation and fibrosis induced by a HFHC diet."

reach
"Specifically, diet-induced hepatic steatosis, inflammation and fibrosis were significantly exacerbated by USP29 deletion and relieved by USP29 overexpression."
| 1

reach
"Hence, USP27X and USP29 promote the production of cGAMP and downstream IFNs to mediate inflammation and autoimmune responses (Guo et al., 2019; Zhang et al., 2020b)."
USP29 affects TP53
1 1 | 7
USP29 deubiquitinates TP53.
1 | 3
USP29 deubiquitinates TP53. 4 / 4
1 | 3

reach
"USP29 can also deubiquitinate p53 in response to oxidative stress."

reach
"For example, USP10 and USP29 can directly deubiquitinate p53 [30,31], while USP5 regulates p53 levels and alters cell growth that is associated with p21 induction [32]."

reach
"USP29 is transcriptionally activated following oxidative stress and then mediates p53 deubiquitination."
USP29 binds TP53.
1 | 1
1 | 1

reach
"In response to oxidative stress, USP29 binds to and stabilizes p53."

No evidence text available
USP29 activates TP53.
| 2
USP29 activates TP53. 2 / 2
| 2

reach
"During stress, USP29 removes the polyubiquitin chain from p53 thus stabilizing it and induces p53 dependent apoptosis."

reach
"This is also consistent with the observations that human USP29 might modulate the protein levels of p53 [XREF_BIBR]."
USP29 increases the amount of TP53.
| 1
USP29 increases the amount of TP53. 1 / 1
| 1

reach
"Moreover, we found that p53 expression was decreased in UKO mice and further increased by USP29 overexpression upon cerebral I/R injury (Figures 6(c) and 6(d))."
USP29 affects SIRT1
| 4 5
USP29 inhibits SIRT1.
| 2 1
USP29 inhibits SIRT1. 3 / 3
| 2 1

reach
"Accordingly, USP29 overexpression-induced SIRT1 inhibition was prevented in mice treated with either PFT-α or miR-34a antagomir (Figure 6(k))."

eidos
"USP29 Inhibits SIRT1 Expression via p53 / miR-34a Axis Finally , we explored the possible mechanism through which USP29 modulated SIRT1 expression ."

eidos
"Based on these results , we demonstrate that USP29 inhibits SIRT1 expression via the p53 / miR-34a axis ."
USP29 increases the amount of SIRT1.
| 2
USP29 increases the amount of SIRT1. 2 / 2
| 2

reach
"Finally, we explored the possible mechanism through which USP29 modulated SIRT1 expression."

reach
"In view of the fact that USP29 is a deubiquitinase and modulates the downstream targets via catalyzing the removal of monoubiquitin or polyubiquitin chains at the posttranscriptional level, therefore, we speculated that SIRT1 expression was not directly modulated by USP29."
USP29 decreases the amount of SIRT1.
| 2
USP29 decreases the amount of SIRT1. 2 / 2
| 2

reach
"As shown in Figures 4(a)–4(d), USP29 inhibition did not affect cerebral SIRT3 expression upon I/R injury but restored cerebral I/R-induced downregulation of SIRT1, while USP29 overexpression further decreased SIRT1 expression in I/R-injured brains."

reach
"Based on these results, we demonstrate that USP29 inhibits SIRT1 expression via the p53/miR-34a axis."
USP29 activates SIRT1.
| 2
USP29 activates SIRT1. 2 / 2
| 2

eidos
"USP29 Inhibits SIRT1 Expression via p53 / miR-34a Axis Finally , we explored the possible mechanism through which USP29 modulated SIRT1 expression ."

eidos
"In view of the fact that USP29 is a deubiquitinase and modulates the downstream targets via catalyzing the removal of monoubiquitin or polyubiquitin chains at the posttranscriptional level , therefore , we speculated that SIRT1 expression was not directly modulated by USP29 ."
| 6

reach
"Collectively, these findings imply that USP29 potentiates I/R-induced oxidative damage and neuronal apoptosis in vitro."

reach
"Our previous study identified a protective effect of SIRT3 against cerebral I/R injury; therefore, we investigated whether USP29 inhibition prevented cerebral I/R injury through upregulating SIRT3 [28]."

reach
"USP29 Inhibition Prevents Cerebral I/R Injury in Mice."

reach
"The findings enable us to believe that USP29 inhibition prevents cerebral I/R injury in mice."

reach
"USP29 Inhibition Diminishes Cerebral I/R-Induced Oxidative Stress and Apoptosis."

reach
"USP29 Potentiates I/R-Induced Oxidative Damage and Neuronal Apoptosis In Vitro."
| 3

reach
"These data suggest that knocking down the endogenous USP29 is sufficient to diminish cerebral I/R-induced oxidative stress and apoptosis."

reach
"For example, USP29 attenuates hepatic ischemia-reperfusion injury by preventing the activation of TAK1 through K63-linked polyubiquitination [21]."

reach
"Thus, our results suggest that USP29 attenuates hepatic I/R injury by inhibiting the inflammatory response and apoptosis.We performed an RNA-sequencing (RNA-Seq) analysis of liver tissues from USP29 knockout mice and controls with I/R injury after 3h, and the results of the KEGG signaling pathway enrichment analysis showed that the MAPK signaling pathway was the most affected after USP29 knockout."
USP29 affects Mice
| 9
USP29 activates Mice.
| 5
USP29 activates Mice. 5 / 5
| 5

reach
"Systemic knockout of Usp29 depleted MYC and HIF1α in MYC-driven neuroblastoma and B cell lymphoma, inhibited critical metabolic targets and significantly prolonged survival of tumor-bearing mice."

reach
"The findings enable us to believe that USP29 inhibition prevents cerebral I/R injury in mice."

reach
"Accordingly, USP29 overexpression-induced SIRT1 inhibition was prevented in mice treated with either PFT-α or miR-34a antagomir (Figure 6(k))."

reach
"And USP29 overexpression also significantly promoted MDA generation in I/R mice (Figure 3(h))."

reach
"Based on the above results, we conclude that USP29 upregulation during cerebral I/R injury potentiates oxidative stress, apoptosis, and neurological dysfunction in mice."
USP29 inhibits Mice.
| 4
USP29 inhibits Mice. 4 / 4
| 4

reach
"In our previous study, we revealed that USP29 promoted tumor cell aerobic glycolysis and glutamine anaplerosis by deubiquitinating and stabilizing the oncogenic MYC family and HIF1α, and systemic knockout of USP29 significantly suppressed tumor progression and prolonged the survival of tumor-bearing mice."

reach
"In addition, SIRT1 activity was increased in USP29-suppressed mice but further decreased in USP29-overexpressed mice (Figure 4(e))."

reach
"Therefore, we tried to clarify whether BMAL1 is required for SIRT1-mediated cerebroprotection in USP29-suppressed mice."

reach
"Moreover, in H/R-stimulated hepatocytes, phosphorylated TAK1 was significantly elevated in the absence of USP29 and significantly decreased in the USP29 overexpressing mice (Figure 6F)."
USP29 affects E7
4 4 | 1
2 2 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
1 1 |

No evidence text available

No evidence text available
E7 binds USP29. 1 / 1
1 |

No evidence text available
1 |

No evidence text available
USP29 binds USP33 and E7. 1 / 1
| 1

sparser
"The Gaussia princeps luciferase protein complementation assay shows that USP15 interacts with the key oncoprotein human papillomavirus (HPV) E6, while USP29 and USP33 bind to E7 protein, suggesting that cellular DUBs impact HPV tumorigenesis by regulating the stability of the viral proteins [ xref ]."
USP29 affects IFNB1
| 8
USP29 decreases the amount of IFNB1.
| 3
USP29 decreases the amount of IFNB1. 3 / 3
| 3

reach
"Interestingly, we found that knockdown of USP29 significantly impaired various cytoplasmic DNA induced expression of IFNB, TNF, and IP10 but had little effect on cGAMP induced expression of IFNB, TNF, and IP10."

reach
"Accordingly, USP29 enhanced ORF9b-mediated IFN-β inhibition, while USP29 alone did not affect IFN-β mRNA levels (Fig. 6D)."

reach
"Knockdown of USP29 by these two shRNAs significantly inhibited HSV-1-induced expression of IFNB, TNF, IL6, and IP10 in THP-1 cells (The # 2 shRNA was used for the following experiments and similar results were observed with # 1 shRNA)."
USP29 inhibits IFNB1.
| 2
USP29 inhibits IFNB1. 2 / 2
| 2

reach
"Based on these data, we next examined the effect of USP29 on ORF9b-mediated IFN-β inhibition."

reach
"In addition, USP29 enhanced ORF9b inhibition of IFN-β and NF-κB activation in response to various stimuli, such as MAVS and Sendai virus (SeV) (Fig. 5C–F)."
USP29 increases the amount of IFNB1.
| 2
USP29-C298A increases the amount of IFNB1. 1 / 1
| 1

reach
"XREF_FIG, reconstitution of wild-type USP29 but not the inactive mutant USP29 (C298A) into Usp29 m/m MLFs fully restored the expression of Ifnb, Ifna4, Tnf, or Il6."
USP29 increases the amount of IFNB1. 1 / 1
| 1

reach
"XREF_FIG, reconstitution of wild-type USP29 but not the inactive mutant USP29 (C298A) into Usp29 m/m MLFs fully restored the expression of Ifnb, Ifna4, Tnf, or Il6."
USP29 activates IFNB1.
| 1
USP29 activates IFNB1. 1 / 1
| 1

reach
"In addition, knockdown of USP29 significantly impaired the production of IFN-beta, IFN-alpha, IL-6, and TNF after infection of HSV-1 or transfection of various DNA ligands."
USP29 affects GST
| 8
| 8

sparser
"In addition, purified GSTUSP29 but not GST could interact with His‐CDK1 in vitro, indicating the direct interaction between USP29 and CDK1 (Figure  xref )."

sparser
"Bacterial‐expressed GST and GSTUSP29 protein bound to Pierce Glutathione Agarose was incubated with PET28A‐TWIST1, purified from the Escherichia coli strain BL21 overnight at 4 °C. Then the beads were washed with PBS four times, followed by western blotting."

sparser
"Figure  xref illustrates that Flag‐tagged TAK1 in cell lysate could be specifically bound and eluted by GSTUSP29 fusion protein but not GST alone."

sparser
"In addition, purified GSTUSP29 but not GST pulled down His‐TWIST1 in vitro, indicating the direct interaction between USP29 and TWIST1 (Figure  xref )."

sparser
"The ubiquitinated TWIST1 protein was then incubated with purified GSTUSP29 WT and C294S protein separately for 4 h at 4 °C, followed by western blotting analysis."

sparser
"The recombinant GSTUSP29 WT and 3A mutant protein were expressed in Escherichia coli strain BL21 and purified using Pierce Glutathione Agarose."

sparser
"Next, Co-IP experiments revealed a direct interaction between USP29 and TAK1 (Fig. xref ), which was further confirmed by GST pull-down assays (Fig. xref )."

sparser
"Purified CDK1 beads were finally incubated with purified GSTUSP29 WT and 3A in kinase buffer (50 m m Tris‐HCl, pH 7.4, 50 m m NaCl, 10 m m MgCl 2 , 10 mm β ‐glycerophosphate, 1 mm dithiothreitol (DTT), and 100 µ m ATP)."
AURKB affects USP29
3 | 3 2
AURKB binds USP29.
3 | 2 2
3 | 2 2

sparser
"USP29 interacts with AURKB and removes its K48-linked polyubiquitination."

sparser
"Moreover, co-immunoprecipitation (Co-IP) using MGC-803 and HGC-27 cell lysates validated the specific interaction between endogenous USP29 and AURKB in gastric cancer cells (Fig.  xref D)."

No evidence text available

reach
"In terms of mechanism, USP29 is transcriptionally activated by FUBP1, and the deregulated USP29 interacts with and stabilizes AURKB, forming a FUBP1-USP29-AURKB axis that enables tumor cell proliferation and survival in gastric cancers."

reach
"Moreover, co-immunoprecipitation (Co-IP) using MGC-803 and HGC-27 cell lysates validated the specific interaction between endogenous USP29 and AURKB in gastric cancer cells (Fig. 2D)."

No evidence text available

No evidence text available
AURKB activates USP29.
| 1
AURKB activates USP29. 1 / 1
| 1

reach
"Consistently, ectopic expression of AURKB also rescued the cell proliferation in USP29 depleted MGC-803 cells (Supplementary Fig. 3B)."

reach
"These data support the notion that USP29 promotes gastric cancer proliferation primarily through stabilization of AURKB.As deregulation of AURKB is observed in different tumors [4], to test whether USP29 is a global regulator of AURKB, we analyzed AURKB protein expression in neuroblastoma SKN-BE2, lung cancer A549 and colorectal cancer HCT116 cells with or without USP29 depletion."

reach
"Considering the important role of AURKB in controlling cell cycle, we reasoned that USP29 may promote gastric cancer cell proliferation by regulating cell cycle progression."

reach
"We next revealed that overexpression of USP29 in gastric cancer cell lines MGC-803 and SNU-216 significantly promoted the tumor cell proliferation (Fig. 1C and Supplementary Fig. 1C)."

reach
"After transfection of si-USP29, the proliferation of CRC cells was evaluated by the cell counting kit-8, colony formation and 5-ethynyl-2'-deoxyuridine assays, and we observed that depletion of USP29 inhibited the proliferation of CRC cells."

eidos
"USP29 depletion produced a pronounced decrease in cell proliferation compared with the scrambled sgRNA control ( p = 0.0189 ) ( Figure 4B ) ."

reach
"Overexpression of USP29 stimulates the proliferation of colorectal cancer cell lines by regulating the activity of the cancer marker nuclear protein Ki67 [30]."

reach
"USP29 knockdown significantly decreased CRC cell proliferation in vitro."
| 1 5
| 1 5

reach
"While USP29 was reported to promote gastric cancer cell migration in vitro, the exact role of USP29 in regulating gastric carcinogenesis remains unknown."

reach
"18 In addition, USP29 was reported to promote gastric cancer cell migration by cooperating with phosphatase SCP1 to stabilize Snail protein."

reach
"Deubiquitinase USP29 promotes cell migration by stabilizing Snail protein ."

reach
"Deubiquitinase USP29 promotes gastric cancer cell migration by cooperating with phosphatase SCP1 to stabilize Snail protein."

reach
"Biologically, ectopic expression of USP29 promotes gastric cancer cell migration, and depletion of Snail abolishes USP29 mediated cell migration; and USP29 can be induced by major EMT and metastatic inducing factors such as TGFbeta, TNFalpha, and hypoxia."

eidos
"Collectively , these data indicate that Snail is a crucial substrate for USP29 to promote cell migration and USP29 / SCP1 complex may be new therapeutic targets to treat metastatic cancer ."
USP29 affects S100A4
| 6
| 6

sparser
"To investigate the involvement of USP29-FSP1 signaling in GC chemoresistance, we initially assessed the protein expression levels of USP29 and FSP1 in various cancer cell lines."

sparser
"After elucidating the function of the USP29-FSP1 axis in GC chemoresistance, we sought to identify the specific E3 ubiquitin ligase responsible for this process."

sparser
"USP29 binds and stabilizes FSP1."

sparser
"To further confirm the interaction between USP29 and FSP1, endogenous co-immunoprecipitation (Co-IP) assays were conducted, demonstrating that reciprocal immunoprecipitation of endogenous USP29 (or FSP1) effectively precipitated FSP1 (or USP29) (Fig.  xref )."

sparser
"Notably, the enzymatic domain (UCH domain) of USP29 was identified as crucial for mediating the USP29-FSP1 interaction (Fig.  xref )."

sparser
"USP29 directly interacts with FSP1, reducing its ubiquitination levels through its DUB activity, ultimately leading to the stabilization of FSP1."
FUBP1 affects USP29
| 5 1
FUBP1 increases the amount of USP29.
| 3
FUBP1 increases the amount of USP29. 3 / 3
| 3

reach
"These data indicated that FUBP1 may directly activate USP29 expression in gastric cancer cells."

reach
"Overexpression of FUBP1 directly activates USP29 transcription in gastric cancers."

reach
"Here, we first depleted FUBP1 expression in gastric cancer cell lines using shRNAs and demonstrated that knockdown of FUBP1 markedly suppressed the gene expression of USP29 in all three cell lines (Fig. 4A and B)."
FUBP1 activates USP29.
| 2 1
FUBP1 activates USP29. 3 / 3
| 2 1

reach
"USP29 activation mediated by FUBP1 promotes AURKB stability and oncogenic functions in gastric cancer."

reach
"In terms of mechanism, USP29 is transcriptionally activated by FUBP1, and the deregulated USP29 interacts with and stabilizes AURKB, forming a FUBP1-USP29-AURKB axis that enables tumor cell proliferation and survival in gastric cancers."

sparser
"In terms of mechanism, USP29 is transcriptionally activated by FUBP1, and the deregulated USP29 interacts with and stabilizes AURKB, forming a FUBP1-USP29-AURKB axis that enables tumor cell proliferation and survival in gastric cancers."
AIMP2 affects USP29
| 5 1
AIMP2 activates USP29.
| 3 1
AIMP2 activates USP29. 4 / 4
| 3 1

reach
"USP29 is induced by JTV1 and FBP driven transcription in response to oxidative stress, and was also shown to be important for the full activation of PUMA and apoptosis [XREF_BIBR]."

reach
"Knockdown of parkin increased the level of AIMP2, leading to ultimately USP29 and MYBBP1A accumulation in SH-SY5Y cells."

sparser
"In addition, a recent study revealed that AIMP2–FBP1 complex transcriptionally activates USP29 in oxidative conditions [ xref ]."

reach
"These results suggest that AIMP2 upregulates USP29 and MYBBP1A in the absence of parkin activity, contributing to PD pathogenesis."
AIMP2 increases the amount of USP29.
| 2
AIMP2 increases the amount of USP29. 2 / 2
| 2

reach
"USP29 is expressed by JTV1 and FBP transcriptional factors."
| PMC

reach
"JTV1 co-activates FBP to induce USP29 transcription and stabilize p53 in response to oxidative stress."
USP29 affects p38
| 5
USP29 inhibits p38.
| 3
USP29 inhibits p38. 3 / 3
| 3

reach
"Taken together, these results suggest that USP29 inhibits the activation of the TAK1-JNK/p38 pathway during hepatic I/R injury."

reach
"USP29 inhibits TAK1-JNK/p38 pathway activation during hepatic I/R injury."

reach
"We found that USP29 knockout significantly promoted TAK1-JNK/p38 pathway activation after the H/R challenge However, this effect was countered by blocking TAK1 activation (Figure 8A)."
USP29 phosphorylates p38.
| 1
USP29 leads to the phosphorylation of p38. 1 / 1
| 1

reach
"In primary mouse hepatocyte injury induced by H/R in vitro, USP29 deficiency promoted phosphorylation of JNK and p38 compared to control cells (Figure 6A), whereas USP29 overexpression inhibited phosphorylation of JNK and p38, ERK phosphorylation was not affected by the USP29 expression (Figure 6B)."
USP29 dephosphorylates p38.
| 1
USP29 leads to the dephosphorylation of p38. 1 / 1
| 1

reach
"In primary mouse hepatocyte injury induced by H/R in vitro, USP29 deficiency promoted phosphorylation of JNK and p38 compared to control cells (Figure 6A), whereas USP29 overexpression inhibited phosphorylation of JNK and p38, ERK phosphorylation was not affected by the USP29 expression (Figure 6B)."
| 4

reach
"In neuroblastoma, we previously revealed that USP29 promotes glycolysis and glutamine catabolism in normoxia and/or hypoxia."

reach
"USP29 has also been shown to promote neuroblastoma progression by upregulating glycolysis and glutamine catabolism (Kang et al., 2023)."

reach
"USP29 was reported to promote tumor cell glycolysis in normoxia or hypoxia by stabilizing HIF1α, and thus, we proposed that USP29 may also regulate de novo nucleotide synthesis under hypoxia.To assess this hypothesis, we depleted endogenous USP29 in SK-N-BE2 cells and then analyzed nucleotide synthesis in cells cultured under hypoxic conditions (1% O ) using C glucose as a tracer."

reach
"Depletion of USP29 significantly reduces glycolysis and lactate production."
| 1

reach
"The absence of USP29, and thus of HIF1alpha transcriptional activity, reduces the levels of aerobic glycolysis and restores sensitivity to Sorafenib in Sorafenib-resistant HCC cells in vitro and in xenograft transplantation mouse models in vivo."
USP29 affects Virulence
| 5
| 5

reach
"For example, aberrant USP29 deubiquitylation could enhance the virulence of SARS-CoV-2 by preventing proteasome degradation of ORF9b (34)."

reach
"34 However, previous studies have reported that Usp29 can enhance SARS-CoV-2 virulence by preventing the proteasomal degradation of ORF9b, and the level of the USP29 mRNA in the peripheral blood mononuclear cells of SARS-CoV-2 patients was higher than that in healthy individuals.35 Interestingly, the change in the USP mRNA levels in human peripheral blood mononuclear cells did not correspond with those in the USP protein levels in the mouse cerebral cortexes."

reach
"The Deubiquitinase USP29 Promotes SARS-CoV-2 Virulence by Preventing Proteasome Degradation of ORF9b."

reach
"Moreover, USP29 promoted the virulence of VSV-eGFP and transcription and replication-competent SARS-CoV-2 virus-like particles (trVLP) by enhancing ORF9b-mediated inhibition of IFN induction and NF-κB activation."

reach
"USP29 increases the virulence of VSV-eGFP and SARS-CoV-2 trVLP.."
USP29 affects USP29
| 1 4
USP29 dephosphorylates USP29.
| 2
USP29-S672A leads to the dephosphorylation of USP29. 1 / 1
| 1

reach
"As shown in Figure 4H and Figure S4I,J (Supporting Information), the single mutation of USP29 S575A, T578A, or S672A only mildly decreased the phosphorylation of USP29, while the phosphorylation‐deficient 3A mutation (S575A/T578A/S672) almost completely abolished it."
USP29-T578A leads to the dephosphorylation of USP29. 1 / 1
| 1

reach
"As shown in Figure 4H and Figure S4I,J (Supporting Information), the single mutation of USP29 S575A, T578A, or S672A only mildly decreased the phosphorylation of USP29, while the phosphorylation‐deficient 3A mutation (S575A/T578A/S672) almost completely abolished it."
USP29 activates USP29.
| 1 1
USP29 activates USP29. 2 / 2
| 1 1

eidos
"Collectively , these data indicate that Snail is a crucial substrate for USP29 to promote cell migration and USP29 / SCP1 complex may be new therapeutic targets to treat metastatic cancer ."

reach
"In addition, USP29 overexpression induced by chemotherapy and oxidative stress treatment can promote chemotherapy resistance by hijacking the USP29/Snail1 axis, which is associated with enhanced cancer stem cell properties, poor prognosis and drug resistance in lung cancer (91)."
USP29 phosphorylates USP29.
| 1
USP29-S575A leads to the phosphorylation of USP29. 1 / 1
| 1

reach
"As shown in Figure 4H and Figure S4I,J (Supporting Information), the single mutation of USP29 S575A, T578A, or S672A only mildly decreased the phosphorylation of USP29, while the phosphorylation‐deficient 3A mutation (S575A/T578A/S672) almost completely abolished it."
USP29 affects TNF
| 5
USP29 decreases the amount of TNF.
| 3
USP29 decreases the amount of TNF. 3 / 3
| 3

reach
"Knockdown of USP29 by these two shRNAs significantly inhibited HSV-1-induced expression of IFNB, TNF, IL6, and IP10 in THP-1 cells (The # 2 shRNA was used for the following experiments and similar results were observed with # 1 shRNA)."

reach
"In our study, USP29 overexpression attenuated CD11b-positive inflammatory cells infiltration and downregulated expression of inflammatory factors Tnf and Il1b and pro-apoptotic factors Bax and Bad."

reach
"Interestingly, we found that knockdown of USP29 significantly impaired various cytoplasmic DNA induced expression of IFNB, TNF, and IP10 but had little effect on cGAMP induced expression of IFNB, TNF, and IP10."
USP29 inhibits TNF.
| 1
USP29 inhibits TNF. 1 / 1
| 1

reach
"We also found that USP29 overexpression in hepatocytes significantly reduced the expression levels of inflammatory factors (Tnf, Il6, Il8 and Ccl2) and inhibited the activation of the NF-κB signaling pathway (Figures 2C, D)."
USP29 activates TNF.
| 1
USP29 activates TNF. 1 / 1
| 1

reach
"In addition, knockdown of USP29 significantly impaired the production of IFN-beta, IFN-alpha, IL-6, and TNF after infection of HSV-1 or transfection of various DNA ligands."
USP29 affects SARS-CoV-2
| 5
| 5

reach
"For example, aberrant USP29 deubiquitylation could enhance the virulence of SARS-CoV-2 by preventing proteasome degradation of ORF9b (34)."

reach
"The Deubiquitinase USP29 Promotes SARS-CoV-2 Virulence by Preventing Proteasome Degradation of ORF9b."

reach
"34 However, previous studies have reported that Usp29 can enhance SARS-CoV-2 virulence by preventing the proteasomal degradation of ORF9b, and the level of the USP29 mRNA in the peripheral blood mononuclear cells of SARS-CoV-2 patients was higher than that in healthy individuals.35 Interestingly, the change in the USP mRNA levels in human peripheral blood mononuclear cells did not correspond with those in the USP protein levels in the mouse cerebral cortexes."

reach
"Moreover, USP29 promoted the virulence of VSV-eGFP and transcription and replication-competent SARS-CoV-2 virus-like particles (trVLP) by enhancing ORF9b-mediated inhibition of IFN induction and NF-κB activation."

reach
"USP29 increases the virulence of VSV-eGFP and SARS-CoV-2 trVLP.."
USP29 affects HIF1A
2 | 3
USP29 binds HIF1A.
2 | 1
2 | 1

No evidence text available

reach
"USP29 interacts with and deubiquitylates HIF1alpha."

No evidence text available
USP29 deubiquitinates HIF1A.
| 1
USP29 deubiquitinates HIF1A. 1 / 1
| 1

reach
"USP29 interacts with and deubiquitylates HIF1alpha."
USP29 activates HIF1A.
| 1
USP29 activates HIF1A. 1 / 1
| 1

reach
"USP29-mediated HIF1alpha stabilization is associated with Sorafenib resistance of hepatocellular carcinoma cells by upregulating glycolysis."
USP29 affects Fibrosis
| 5
| 5

reach
"USP29 knockout accelerates hepatic steatosis, inflammation and fibrosis induced by a HFHC diet."

reach
"In conclusion, these results indicate that USP29 knockout accelerates hepatic steatosis, inflammation and fibrosis induced by a HFHC diet."

reach
"Specifically, diet-induced hepatic steatosis, inflammation and fibrosis were significantly exacerbated by USP29 deletion and relieved by USP29 overexpression."

reach
"Hepatic steatosis, inflammation and fibrosis were significantly exacerbated by USP29 deletion and relieved by USP29 overexpression."

reach
"Moreover, genetic knockout of USP29 in mice exacerbated transverse aortic constriction-induced heart hypertrophy, dysfunction, and fibrosis, whereas overexpression of USP29 in cardiomyocytes using adeno-associated virus 9 effectively attenuated the hypertrophic response."
| 1 4
| 1 4

eidos
"USP29 is a member of the USP family and functions as an oncogene to promote tumorigenesis in some human tumors ."

reach
"Usp29 knockout suppresses BaP-induced gastric carcinogenesis."

reach
"USP29 is a member of the USP family and functions as an oncogene to promote tumorigenesis in some human tumors."

reach
"USP29 upregulation enhances the cancer stem cell-like characteristics in lung adenocarcinoma cells to promote tumorigenesis in athymic nude mice."

reach
"Conversely, USP37 and USP29 promote tumorigenesis by stabilizing c-Myc through deubiquitination [22, 23]."
MYC affects USP29
1 | 4
1 | 4

sparser
"To validate the mass spectrometry data, we performed reciprocal immunoprecipitation with co-expression of Flag-AURKB and Myc-USP29 in 293T cells and confirmed associations between these both proteins (Fig.  xref C)."

sparser
"HEK 293 T cells were transfected with both Myc-USP29 (wild-type or with C223S mutation), HA-Ub, and Flag-FTO to confirm that USP7 controls the ubiquitination of FTO."

No evidence text available

sparser
"To examine which kind of polyubiquitin chains USP29 removes from AURKB, we co-transfected 293T cells with Flag-AURKB, Myc-USP29 and HA-tagged ubiquitin mutants retaining a single lysine residue, and uncovered that USP29 specifically deconjugated the K48-linked polyubiquitin chains (Fig.  xref G), a well-established destruction signal to trigger 26 S proteasome-mediated proteolysis [ xref ]."

sparser
"To further explore this notion, Flag-AURKB, Myc-USP29, HA-tagged ubiquitin (WT) or K48R mutant were co-transfected into 293T cells, and the results showed that USP29 failed to catalyze removal of K48R-linked polyubiquitin chains from AURKB (Fig.  xref H)."
HIF1 affects USP29
| 5
HIF1 binds USP29.
| 2
| 2

reach
"To this end, we first examined a physical interaction between USP29 and HIF1α."

reach
"Indeed, we found that USP29 binds HIF1α in both Huh7-IR and Huh7-CR Sorafenib-resistant cells (Fig. 3a, b)."
HIF1 activates USP29.
| 2
HIF1 activates USP29. 2 / 2
| 2

reach
"Immunohistochemical staining of USP29 and HIF1α on tumor sections confirmed that the siRNA-mediated knockdown of USP29 not only efficiently depleted USP29 but also reduced HIF1α levels (Fig. 6d)."

reach
"However, upon siRNA-mediated depletion of USP29, the loss of HIF1α protein was substantially accelerated, further supporting the key role of USP29 in stabilizing HIF1α."
HIF1 inhibits USP29.
| 1
HIF1 inhibits USP29. 1 / 1
| 1

reach
"However, upon siRNA-mediated depletion of USP29, the loss of HIF1α protein was substantially accelerated, further supporting the key role of USP29 in stabilizing HIF1α."
FBP1 affects USP29
| 4 1
FBP1 activates USP29.
| 2 1
FBP1 activates USP29. 3 / 3
| 2 1

sparser
"In addition, a recent study revealed that AIMP2–FBP1 complex transcriptionally activates USP29 in oxidative conditions [ xref ]."

reach
"USP29 is induced by JTV1 and FBP driven transcription in response to oxidative stress, and was also shown to be important for the full activation of PUMA and apoptosis [XREF_BIBR]."

reach
"It will be interesting to examine whether DX2 also competes with AIMP2 and p38 for binding to FBP1 and inhibits FBP1 mediated induction of USP29, which in turn, may have destabilizing effect on p53."
FBP1 increases the amount of USP29.
| 2
FBP1 increases the amount of USP29. 2 / 2
| 2

reach
"FBP1 promotes transcription of the USP29 gene through binding to FUSE."

reach
"JTV1 co-activates FBP to induce USP29 transcription and stabilize p53 in response to oxidative stress."
CLSPN affects USP29
2 | 1 1
2 | 1 1

No evidence text available

No evidence text available

reach
"USP29 interacts with Claspin and is able to deubiquitinate it both in vivo and in vitro."

trips
"USP29 interacts with Claspin and is able to deubiquitinate it both in vivo and in vitro."
Cadmium dichloride increases the amount of USP29.
2 |
Cadmium dichloride increases the amount of USP29. 2 / 2
2 |

No evidence text available

No evidence text available
Cadmium dichloride decreases the amount of USP29.
2 |
Cadmium dichloride decreases the amount of USP29. 2 / 2
2 |

No evidence text available

No evidence text available
USP29 affects sorafenib
| 4
| 4

reach
"USP29 promotes sorafenib resistance in vivo."

reach
"Gao et al. (84) found high USP29, HIF1α and GLUT1 levels in sorafenib-resistant PDX tumours, and follow-up research revealed that USP29-induced sorafenib resistance by mediating HIF1α stabilisation and upregulated glycolysis."

reach
"USP29 promotes sorafenib resistance in HCC cells by stabilizing HIF1α [22]."

reach
"Together, the data demonstrate that, as a DUB of HIF1alpha, USP29 promotes Sorafenib resistance in HCC cells, in parts by upregulating glycolysis, thereby opening new avenues for therapeutically targeting Sorafenib-resistant HCC in patients."
USP29 affects cellular antiviral responses
| 4
USP29 activates cellular antiviral responses. 4 / 4
| 4

eidos
"Author Correction : USP29 maintains the stability of cGAS and promotes cellular antiviral responses and autoimmunity Correction to : Cell Research 10.1038 / s41422-020-0341-6 , published online 26 May 2020 We just found several mistakes and typos in the paper published on May 26 , 2020 ."

eidos
"These data collectively suggest that USP29 deficiency restricts cellular antiviral responses against HSV-1 ."

eidos
"USP29 maintains the stability of cGAS and promotes cellular antiviral responses and autoimmunity Cyclic GMP-AMP synthase ( cGAS ) is an essential sensor of cytosolic DNA and critically mediates innate immune responses and autoimmunity ."

eidos
"These data suggest that USP29 promotes cellular antiviral responses in a manner dependent on its DUB activity ."
USP29 affects K48
| 4
USP29 binds CGAS and K48. 3 / 3
| 3

reach
"Mechanistically, USP29 constitutively interacts with cGAS, deconjugates K48 linked polyubiquitin chains from cGAS and stabilizes cGAS in uninfected cells or after HSV-1 infection."

reach
"[208] Recently, a study reported that USP29 interacts with cGAS, hydrolyzes K48 linked polyubiquitin chains on cGAS, and stabilizes cGAS in uninfected cells, or after HSV-1 stimulation."

reach
"USP29 constitutively interacts with cGAS and deconjugates K48 linked polyubiquitin chains from cGAS in the absence or presence of HSV-1 infection, which stabilizes cGAS and promotes cGAS mediated cellular antiviral responses."
USP29 binds ACSL5 and K48. 1 / 1
| 1

reach
"USP29 directly interacts with ACSL5 and stabilizes ACSL5 through the suppression of K48-linked ubiquitination.."
USP29 affects HIF1
| 4
USP29 binds HIF1.
| 2
| 2

reach
"To this end, we first examined a physical interaction between USP29 and HIF1α."

reach
"Indeed, we found that USP29 binds HIF1α in both Huh7-IR and Huh7-CR Sorafenib-resistant cells (Fig. 3a, b)."
USP29 deubiquitinates HIF1.
| 1
USP29 leads to the deubiquitination of HIF1. 1 / 1
| 1

reach
"The removal of ubiquitin on HIF1α seemed to be directly catalyzed by USP29, since a catalytically inactive form of USP29 (CA) failed to reduce ubiquitination of HIF1α (Fig. 3d) and, as a consequence, the CA form of USP29 was unable to stabilize HIF1α."
USP29 activates HIF1.
| 1
USP29 activates HIF1. 1 / 1
| 1

reach
"A recent study has also characterized that USP29-mediated HIF1α stabilization promoted sorafenib resistance in HCC cells, partly through up-regulation of glycolysis (37)."
USP29 affects CTDSP1
3 | 1
3 | 1

No evidence text available

No evidence text available

No evidence text available

sparser
"The molecular mechanism underlying this stabilization involves the specific interaction between USP29 and SCP1, leading to the formation of a USP29/SCP1 complex that facilitates Snail deubiquitination ( xref )."
USP29 affects CDC25A
| 2 2
USP29 binds CDC25A.
| 2
| 2

sparser
"To further investigate the physical association of Cdc25A and USP29, HEK293 cells were co-transfected with Myc-Cdc25A and GFP-USP29 and subjected to immunoprecipitation with anti-Myc or -GFP antibodies, followed by Western blotting with reciprocal antibodies."

sparser
"Thus, our data indicated that Cdc25A and USP29 bound to each other and were co-localized."
USP29 deubiquitinates CDC25A.
| 1
USP29 leads to the deubiquitination of CDC25A. 1 / 1
| 1

reach
"The presence of USP29 effectively blocked polyubiquitination of Cdc25A and extended its half-life."
USP29 activates CDC25A.
| 1
USP29 activates CDC25A. 1 / 1
| 1

reach
"17 In a cervical cancer study, the overexpression of USP29 enables the stabilization of the cell division cycle 25A (Cdc25A) and enhances the oncogenic potential of HeLa cells."
K48 affects USP29
| 4
USP29 binds CGAS and K48. 3 / 3
| 3

reach
"Mechanistically, USP29 constitutively interacts with cGAS, deconjugates K48 linked polyubiquitin chains from cGAS and stabilizes cGAS in uninfected cells or after HSV-1 infection."

reach
"[208] Recently, a study reported that USP29 interacts with cGAS, hydrolyzes K48 linked polyubiquitin chains on cGAS, and stabilizes cGAS in uninfected cells, or after HSV-1 stimulation."

reach
"USP29 constitutively interacts with cGAS and deconjugates K48 linked polyubiquitin chains from cGAS in the absence or presence of HSV-1 infection, which stabilizes cGAS and promotes cGAS mediated cellular antiviral responses."
USP29 binds ACSL5 and K48. 1 / 1
| 1

reach
"USP29 directly interacts with ACSL5 and stabilizes ACSL5 through the suppression of K48-linked ubiquitination.."
CTDSP1 affects USP29
3 | 1
3 | 1

No evidence text available

No evidence text available

No evidence text available

sparser
"The molecular mechanism underlying this stabilization involves the specific interaction between USP29 and SCP1, leading to the formation of a USP29/SCP1 complex that facilitates Snail deubiquitination ( xref )."
9b affects USP29
3 | 1
3 | 1

reach
"We performed forward and reverse IP assays, which showed the interaction between USP29 and ORF9b (Fig. S3A–B)."

No evidence text available

No evidence text available

No evidence text available
3 |
Pirinixic acid decreases the amount of USP29.
2 |
Pirinixic acid decreases the amount of USP29. 2 / 2
2 |

No evidence text available

No evidence text available
Pirinixic acid activates USP29.
1 |

No evidence text available
VIRMA affects USP29
1 | 2
VIRMA binds USP29.
1 | 1
1 | 1

No evidence text available

reach
"Co-immunoprecipitation confirmed the binding of USP29 to KIAA1429."
VIRMA inhibits USP29.
| 1
VIRMA inhibits USP29. 1 / 1
| 1

reach
"Moreover, overexpression of KIAA1429 or SOX8 reversed the inhibitory effects of USP29 depletion on CRC cell proliferation."

reach
"USP29 promotes nucleotide biosynthesis under hypoxia."

reach
"USP29 deficiency decreases nucleotide biosynthesis."

reach
"These results indicated that USP29 may promote nucleotide biosynthesis by regulating the MYC and E2F downstream pathways."
| 3
USP29 activates malonaldehyde.
| 2
| 2

reach
"And USP29 overexpression also significantly promoted MDA generation in I/R mice (Figure 3(h))."

reach
"As expected, the silencing of USP29 significantly inhibited the migratory ability and invasiveness of MDA‐MB‐231 (Figure 3B,C) and BT549 cells (Figure S3G–I, Supporting Information) without affecting cell growth (Figure S3E,F, Supporting Information)."
USP29 inhibits malonaldehyde.
| 1

reach
"11 We next evaluated the effect of USP29 on the enrichment and activity of CSCs in vivo, limiting dilution assay (4 × 10 , 4 × 10 , or 1 × 10 cells) of MDA‐MB‐231 cells stably expressing control or USP29 shRNA were subcutaneously injected into nude mice."

reach
"And the simultaneous depletion of TWIST1 largely reversed such an effect induced by USP29 overexpression (Figure S3A,B, Supporting Information), indicating the driven EMT process by USP29 is mediated by TWIST1."

reach
"19 In addition, the transcription of USP29 in gastric cancer cells could also be induced by TGF‐β, TNFα, and hypoxia, which were reported to be present in the tumor microenvironment and promote EMT and metastasis."

reach
"For example, USP3, USP11, and USP29 stimulate EMT in glioblastoma cells, as well as in ovarian or gastric cancer cells, respectively, by stabilizing Snail [80–82] ."
USP29 affects cerebral R injury
| 3
USP29 activates cerebral R injury. 3 / 3
| 3

eidos
"Our previous study identified a protective effect of SIRT3 against cerebral I / R injury ; therefore , we investigated whether USP29 inhibition prevented cerebral I / R injury through upregulating SIRT3 [ 28 ] ."

eidos
"Our findings for the first time identify that USP29 upregulation during cerebral I / R may contribute to oxidative stress , neuronal apoptosis , and the progression of cerebral I / R injury and that inhibition of USP29 may help to develop novel therapeutic strategies to treat cerebral I / R injury ."

eidos
"Generally , our findings for the first time identify that USP29 upregulation during cerebral I / R may contribute to oxidative stress , neuronal apoptosis , and the progression of cerebral I / R injury and that inhibition of USP29 may help to develop novel therapeutic strategies to treat cerebral I / R injury ."
USP29 affects VIRMA
1 | 2
USP29 binds VIRMA.
1 | 1
1 | 1

No evidence text available

reach
"Co-immunoprecipitation confirmed the binding of USP29 to KIAA1429."
USP29 increases the amount of VIRMA.
| 1
USP29 increases the amount of VIRMA. 1 / 1
| 1

reach
"Mechanically, USP29 mediated deubiquitination to stabilize the protein levels of KIAA1429, and KIAA1429 promoted the stability of SOX8 mRNA through m6A modification."
USP29 affects VIM
| 3
USP29 increases the amount of VIM. 3 / 3
| 3

reach
"As shown in Figure S3A,B (Supporting Information), the stable expression of USP29 decreased the expression of epithelial marker E‐cadherin and increased the expression of mesenchymal marker Vimentin."

reach
"Of note, the depletion of USP29 in both MDA‐MB‐231 and BT549 cells significantly decreased the expression of TWIST1 and Vimentin, and increased the expression of epithelial marker E‐cadherin (Figure 3A; Figure S3C,D, Supporting Information), while the reconstitution of TWIST1 in USP29‐deficient cells could dramatically rescue such a change (Figure 3A)."

reach
"As shown in Figure S6G,H (Supporting Information), the stable overexpression of USP29 in luminal breast cancer cell MCF‐7 decreased the level of epithelial marker E‐cadherin and increased the expression of mesenchymal marker Vimentin, whereas the 3A mutant failed to induce such phenotypes."
USP29 affects TAK1-JNK
| 3
USP29 inhibits TAK1-JNK. 3 / 3
| 3

reach
"USP29 inhibits TAK1-JNK/p38 pathway activation during hepatic I/R injury."

reach
"Taken together, these results suggest that USP29 inhibits the activation of the TAK1-JNK/p38 pathway during hepatic I/R injury."

reach
"We found that USP29 knockout significantly promoted TAK1-JNK/p38 pathway activation after the H/R challenge However, this effect was countered by blocking TAK1 activation (Figure 8A)."
USP29 affects SLC27A2
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
| 2

reach
"Since USP29 increases cancer cell migration and invasion through TWIST1 in vitro, we further investigated USP29 function in TNBC metastasis in vivo."

reach
"As expected, the silencing of USP29 significantly inhibited the migratory ability and invasiveness of MDA‐MB‐231 (Figure 3B,C) and BT549 cells (Figure S3G–I, Supporting Information) without affecting cell growth (Figure S3E,F, Supporting Information)."

reach
"And the reconstitution of TWIST1 largely restored the decreased migratory ability and invasiveness caused by USP29 deficiency (Figure 3B,C; Figure S3H,I, Supporting Information)."
USP29 affects NFE2L2
2 | 1
USP29 binds NFE2L2.
2 |
2 |

No evidence text available

No evidence text available
USP29 deubiquitinates NFE2L2.
| 1
USP29 deubiquitinates NFE2L2. 1 / 1
| 1

reach
"For example, USP29 acts as a traumatic spinal cord injury protector by deubiquitinating NRF2 (Nuclear erythroid 2-related factor 2), thereby stabilizing NRF2 function and regulating microglia/macrophage polarization (9)."
USP29 affects MYBBP1A
| 3
| 3

sparser
"To confirm whether USP29 binds to MYBBP1A, Flag-tagged MYBBP1A and HA-tagged USP29 were co-transfected into SH-SY5Y cells and Flag-tagged MYBBP1A was immunoprecipitated, showing that Flag-MYBBP1A pulled down HA-USP29 ( xref B)."

sparser
"We observed that there was no brain-region-specific interaction between USP29 and MYBBP1A, indicating that MYBBP1A might be an authentic physiological substrate of USP29 ( xref E)."

sparser
"USP29 Binds to MYBBP1A."
SLC27A2 affects USP29
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
MYBBP1A affects USP29
| 3
| 3

sparser
"To confirm whether USP29 binds to MYBBP1A, Flag-tagged MYBBP1A and HA-tagged USP29 were co-transfected into SH-SY5Y cells and Flag-tagged MYBBP1A was immunoprecipitated, showing that Flag-MYBBP1A pulled down HA-USP29 ( xref B)."

sparser
"We observed that there was no brain-region-specific interaction between USP29 and MYBBP1A, indicating that MYBBP1A might be an authentic physiological substrate of USP29 ( xref E)."

sparser
"USP29 Binds to MYBBP1A."
K48 affects CGAS
| 3
USP29 binds CGAS and K48. 3 / 3
| 3

reach
"Mechanistically, USP29 constitutively interacts with cGAS, deconjugates K48 linked polyubiquitin chains from cGAS and stabilizes cGAS in uninfected cells or after HSV-1 infection."

reach
"[208] Recently, a study reported that USP29 interacts with cGAS, hydrolyzes K48 linked polyubiquitin chains on cGAS, and stabilizes cGAS in uninfected cells, or after HSV-1 stimulation."

reach
"USP29 constitutively interacts with cGAS and deconjugates K48 linked polyubiquitin chains from cGAS in the absence or presence of HSV-1 infection, which stabilizes cGAS and promotes cGAS mediated cellular antiviral responses."
HIF1A affects USP29
2 | 1
2 | 1

No evidence text available

reach
"USP29 interacts with and deubiquitylates HIF1alpha."

No evidence text available
CGAS affects K48, and USP29
| 3
USP29 binds CGAS and K48. 3 / 3
| 3

reach
"Mechanistically, USP29 constitutively interacts with cGAS, deconjugates K48 linked polyubiquitin chains from cGAS and stabilizes cGAS in uninfected cells or after HSV-1 infection."

reach
"[208] Recently, a study reported that USP29 interacts with cGAS, hydrolyzes K48 linked polyubiquitin chains on cGAS, and stabilizes cGAS in uninfected cells, or after HSV-1 stimulation."

reach
"USP29 constitutively interacts with cGAS and deconjugates K48 linked polyubiquitin chains from cGAS in the absence or presence of HSV-1 infection, which stabilizes cGAS and promotes cGAS mediated cellular antiviral responses."
2,3,7,8-tetrachlorodibenzodioxine decreases the amount of USP29.
2 |
2 |

No evidence text available

No evidence text available
2,3,7,8-tetrachlorodibenzodioxine increases the amount of USP29.
1 |
1 |

No evidence text available

eidos
"Biologically , ectopic expression of USP29 promotes gastric cancer cell migration , and depletion of Snail abolishes USP29-mediated cell migration ; and USP29 can be induced by major EMT and metastatic inducing factors such as TGFbeta , TNFalpha , and hypoxia ."

reach
"Biologically, ectopic expression of USP29 promotes gastric cancer cell migration, and depletion of Snail abolishes USP29 mediated cell migration; and USP29 can be induced by major EMT and metastatic inducing factors such as TGFbeta, TNFalpha, and hypoxia."
2 |
Cadmium atom increases the amount of USP29.
1 |
Cadmium atom increases the amount of USP29. 1 / 1
1 |

No evidence text available
Cadmium atom decreases the amount of USP29.
1 |
Cadmium atom decreases the amount of USP29. 1 / 1
1 |

No evidence text available
Bisphenol F affects USP29
2 |
Bisphenol F increases the amount of USP29.
1 |
Bisphenol F increases the amount of USP29. 1 / 1
1 |

No evidence text available
Bisphenol F demethylates USP29.
1 |
Bisphenol F demethylates USP29. 1 / 1
1 |

No evidence text available
Bisphenol A affects USP29
2 |
Bisphenol A decreases the amount of USP29. 2 / 2
2 |

No evidence text available

No evidence text available
ZEB1 affects USP29
2 |
2 |

No evidence text available

No evidence text available

reach
"The Deubiquitinase USP29 Promotes SARS-CoV-2 Virulence by Preventing Proteasome Degradation of ORF9b."

reach
"For example, aberrant USP29 deubiquitylation could enhance the virulence of SARS-CoV-2 by preventing proteasome degradation of ORF9b (34)."
USP29 affects polyubiquitin chains
| 1 1
USP29 inhibits polyubiquitin chains. 2 / 2
| 1 1

reach
"Because USP29 interacts with cGAS and its enzyme activity is required for regulating antiviral signaling, we investigated whether USP29 eliminated polyubiquitin chains from cGAS."

eidos
"USP29 deubiquitinates cGAS in the presence or absence of HSV-1 infection Because USP29 interacts with cGAS and its enzyme activity is required for regulating antiviral signaling , we investigated whether USP29 eliminated polyubiquitin chains from cGAS ."
USP29 affects pathways
| 2
USP29 inhibits pathways.
| 1
USP29 inhibits pathways. 1 / 1
| 1

sparser
"A Venn diagram revealed that 16 pathways were inhibited by USP29-KO but promoted by USP29 overexpression."
USP29 activates pathways.
| 1
USP29 activates pathways. 1 / 1
| 1

sparser
"In addition, we found that pathways related to inflammation were activated by USP29-KO but inhibited by USP29 overexpression ( xref )."
USP29 affects neuronal apoptosis
| 2
USP29 activates neuronal apoptosis. 2 / 2
| 2

eidos
"Generally , our findings for the first time identify that USP29 upregulation during cerebral I / R may contribute to oxidative stress , neuronal apoptosis , and the progression of cerebral I / R injury and that inhibition of USP29 may help to develop novel therapeutic strategies to treat cerebral I / R injury ."

eidos
"Our findings for the first time identify that USP29 upregulation during cerebral I / R may contribute to oxidative stress , neuronal apoptosis , and the progression of cerebral I / R injury and that inhibition of USP29 may help to develop novel therapeutic strategies to treat cerebral I / R injury ."
USP29 affects lactate
| 2
USP29 activates lactate. 2 / 2
| 2

reach
"In a previous study, we reported that the depletion of USP29 in tumor cells significantly inhibited glucose consumption and lactate production."

reach
"Depletion of USP29 significantly reduces glycolysis and lactate production."
| 2

reach
"To further substantiate our conclusion that USP29 positively regulates antiviral immunity by stabilizing cGAS, we reconstituted an empty vector or cGAS into Usp29 +/+ or Usp29 m/m MLFs and examined HSV-1-triggered signaling in these cells."

reach
"46 In this study, we demonstrated that USP29 deconjugated K48 linked polyubiquitin chains from K271 of cGAS in resting cells or after HSV-1 infection, which stabilized cGAS to produce cGAMP and activate MITA mediated antiviral immunity as well as autoimmunity (Supplementary information, FigS7e)."
USP29 affects glucose
| 2
USP29 inhibits glucose.
| 1
| 1

reach
"ACSL5 overexpression significantly attenuated the increased body weight, blood glucose and glucose tolerance induced by USP29 deletion (Supplementary Fig. 7B–D)."
USP29 activates glucose.
| 1
USP29 activates glucose. 1 / 1
| 1

reach
"In a previous study, we reported that the depletion of USP29 in tumor cells significantly inhibited glucose consumption and lactate production."

reach
"USP29 alleviates hepatocyte lipid deposition and inflammation and promotes the fatty acid degradation pathway."

reach
"These results suggest that USP29 significantly alleviates PAOA-induced lipid accumulation and inflammation in hepatocytes and promotes the fatty acid degradation pathway."
USP29 affects colony
| 2
USP29 activates colony. 2 / 2
| 2

eidos
"We also demonstrated that USP29 depletion hampers the colony formation and increases apoptosis of HCT116 cells ."

eidos
"USP29 depletion produced a significant reduction in colony numbers compared with the scrambled sgRNA control ( p = 0.0073 ) , and reconstitution of USP29 increased the colony numbers compared with the USP29-depleted group ( p = 0.0353 ) ( Figure 4C ) ."
USP29 affects ZEB1
2 |
2 |

No evidence text available

No evidence text available
USP29 affects VSV-eGFP
| 2
USP29 activates VSV-eGFP. 2 / 2
| 2

reach
"USP29 increases the virulence of VSV-eGFP and SARS-CoV-2 trVLP.."

reach
"Moreover, USP29 promoted the virulence of VSV-eGFP and transcription and replication-competent SARS-CoV-2 virus-like particles (trVLP) by enhancing ORF9b-mediated inhibition of IFN induction and NF-κB activation."
USP29 affects TRIM38
| 2
| 1

sparser
"Interestingly, we found that TRIM38 constitutively interacts with cGAS but not with USP29, whereas USP29 constitutively interacts with cGAS but not with TRIM38 (Supplementary information, Fig.  xref ), indicating that TRIM38 and USP29 independently interacts with and regulates cGAS."
TRIM38 binds USP29 and cGAS. 1 / 1
| 1

sparser
"In addition, we found that TRIM38 and USP29 interacts with cGAS independently, suggesting that TRIM38-mediated sumoylation and USP29-mediated deubiquitination of cGAS cooperatively regulate the stability of cGAS."
USP29 affects TREX1
| 2
USP29 inhibits TREX1.
| 1
USP29 inhibits TREX1. 1 / 1
| 1

reach
"In addition, knockout of USP29 in Trex1 -/- background rescued developmental retardation of Trex1 -/- mice."
USP29 activates TREX1.
| 1
USP29 activates TREX1. 1 / 1
| 1

reach
"Consistently, knockout of USP29 results in constitutive degradation of cGAS and eliminates the autoimmune phenotypes in Trex1 -/- mice."
USP29 affects TNBC
| 2
USP29 activates TNBC. 2 / 2
| 2

reach
"Our results demonstrate USP29 promotes tumor progression in TNBC through TWIST1."

reach
"Taken together, these results suggest that USP29 is a bona fide DUB targeting TWIST1 protein for deubiquitination and stabilization.2.3 USP29 Promotes Tumor Progression of TNBC through Stabilizing TWIST1."
| 2

eidos
"Our findings for the first time identify that USP29 upregulation during cerebral I / R may contribute to oxidative stress , neuronal apoptosis , and the progression of cerebral I / R injury and that inhibition of USP29 may help to develop novel therapeutic strategies to treat cerebral I / R injury ."

eidos
"Generally , our findings for the first time identify that USP29 upregulation during cerebral I / R may contribute to oxidative stress , neuronal apoptosis , and the progression of cerebral I / R injury and that inhibition of USP29 may help to develop novel therapeutic strategies to treat cerebral I / R injury ."
USP29 affects OGD/R
| 2
USP29 activates OGD/R. 2 / 2
| 2

reach
"As shown in Figure S1A-B, the survival was decreased, while LDH releases were enhanced in the primary cortical neurons upon OGD/R stimulation, which were attenuated by USP29 silence."

reach
"We also found that USP29 knockdown significantly suppressed OGD/R-induced increases of caspase-3 activity (Figure S1C)."
USP29 affects NFKBIA
| 2
USP29 leads to the phosphorylation of NFKBIA. 1 / 1
| 1

reach
"In addition, the production of IFN-beta or TNF and phosphorylation of IRF3 and IkappaBalpha was rescued by reconstitution of USP29 but not USP29 (C298A) into Usp29 m/m MLFs."
USP29-C298A leads to the phosphorylation of NFKBIA. 1 / 1
| 1

reach
"In addition, the production of IFN-beta or TNF and phosphorylation of IRF3 and IkappaBalpha was rescued by reconstitution of USP29 but not USP29 (C298A) into Usp29 m/m MLFs."
USP29 affects MKI67
| 2
| 1

sparser
"Our results showed that USP29 and Ki67 are not binding each other ( xref ), suggesting that the expression of USP29 increases Ki67 expression in colon cancer cells without interacting with Ki67."
HCT116 binds USP29 and MKI67. 1 / 1
| 1

sparser
"We next checked the interactions between endogenous USP29 and Ki67 in HCT116 cells."
USP29 affects JNK
| 2
USP29 phosphorylates JNK.
| 1
USP29 leads to the phosphorylation of JNK. 1 / 1
| 1

reach
"In primary mouse hepatocyte injury induced by H/R in vitro, USP29 deficiency promoted phosphorylation of JNK and p38 compared to control cells (Figure 6A), whereas USP29 overexpression inhibited phosphorylation of JNK and p38, ERK phosphorylation was not affected by the USP29 expression (Figure 6B)."
USP29 dephosphorylates JNK.
| 1
USP29 leads to the dephosphorylation of JNK. 1 / 1
| 1

reach
"In primary mouse hepatocyte injury induced by H/R in vitro, USP29 deficiency promoted phosphorylation of JNK and p38 compared to control cells (Figure 6A), whereas USP29 overexpression inhibited phosphorylation of JNK and p38, ERK phosphorylation was not affected by the USP29 expression (Figure 6B)."
USP29 affects Interferon
| 1 1
USP29 inhibits Interferon.
| 1
| 1

eidos
"USP29 interacts with the carboxyl end of ORF9b and removes ubiquitin chains from the protein , thereby inhibiting type I interferon ( IFN ) induction and NF-kappaB activation ."
USP29 activates Interferon.
| 1
| 1

reach
"USP29 enhances ORF9b-mediated type I IFN and NF-kappaB inhibition.."
USP29 affects IRF3
| 2
USP29-C298A leads to the phosphorylation of IRF3. 1 / 1
| 1

reach
"In addition, the production of IFN-beta or TNF and phosphorylation of IRF3 and IkappaBalpha was rescued by reconstitution of USP29 but not USP29 (C298A) into Usp29 m/m MLFs."
USP29 leads to the phosphorylation of IRF3. 1 / 1
| 1

reach
"In addition, the production of IFN-beta or TNF and phosphorylation of IRF3 and IkappaBalpha was rescued by reconstitution of USP29 but not USP29 (C298A) into Usp29 m/m MLFs."
USP29 affects IL6
| 2
USP29 decreases the amount of IL6.
| 1
USP29 decreases the amount of IL6. 1 / 1
| 1

reach
"Knockdown of USP29 by these two shRNAs significantly inhibited HSV-1-induced expression of IFNB, TNF, IL6, and IP10 in THP-1 cells (The # 2 shRNA was used for the following experiments and similar results were observed with # 1 shRNA)."
USP29 activates IL6.
| 1
USP29 activates IL6. 1 / 1
| 1

reach
"In addition, knockdown of USP29 significantly impaired the production of IFN-beta, IFN-alpha, IL-6, and TNF after infection of HSV-1 or transfection of various DNA ligands."
USP29 affects Hypoxia
| 2
USP29 activates Hypoxia. 2 / 2
| 2

reach
"In neuroblastoma, we previously revealed that USP29 promotes glycolysis and glutamine catabolism in normoxia and/or hypoxia."

reach
"USP29 was reported to promote tumor cell glycolysis in normoxia or hypoxia by stabilizing HIF1α, and thus, we proposed that USP29 may also regulate de novo nucleotide synthesis under hypoxia.To assess this hypothesis, we depleted endogenous USP29 in SK-N-BE2 cells and then analyzed nucleotide synthesis in cells cultured under hypoxic conditions (1% O ) using C glucose as a tracer."
USP29 affects ELAVL1
1 | 1
1 | 1

No evidence text available

sparser
"Furthermore, RIP-qPCR experiments were conducted to assess the interaction between ELAVL1 and USP29 mRNA, which revealed that ELAVL1 significantly enriched USP29 mRNA compared to the IgG control, indicating a direct interaction between ELAVL1 and USP29 mRNA (Fig. xref )."
USP29 affects Death
| 2
USP29 inhibits Death.
| 1
USP29 inhibits Death. 1 / 1
| 1

reach
"As shown in Figure S2A-B, USP29 silence dramatically prevented OGD/R-induced neuronal injury and death, but not in those with siSIRT1 transfection."
USP29 activates Death.
| 1
USP29 activates Death. 1 / 1
| 1

reach
"As shown in Figure S2A-B, USP29 silence dramatically prevented OGD/R-induced neuronal injury and death, but not in those with siSIRT1 transfection."
USP29 affects CXCL10
| 2
USP29 decreases the amount of CXCL10. 2 / 2
| 2

reach
"Interestingly, we found that knockdown of USP29 significantly impaired various cytoplasmic DNA induced expression of IFNB, TNF, and IP10 but had little effect on cGAMP induced expression of IFNB, TNF, and IP10."

reach
"Knockdown of USP29 by these two shRNAs significantly inhibited HSV-1-induced expression of IFNB, TNF, IL6, and IP10 in THP-1 cells (The # 2 shRNA was used for the following experiments and similar results were observed with # 1 shRNA)."
USP29 affects CRC
| 2
USP29 activates CRC. 2 / 2
| 2

reach
"USP29 knockdown significantly decreased CRC cell proliferation in vitro."

reach
"In conclusion, our study shows that elevated expression of USP29 promotes malignancy in CRC, suggesting that USP29 could be a promising target for colon cancer therapy."
USP29 affects CASP3
| 2
USP29 activates CASP3. 2 / 2
| 2

reach
"We also found that USP29 knockdown significantly suppressed OGD/R-induced increases of caspase-3 activity (Figure S1C)."

reach
"And SIRT1 knockout also blocked USP29 inhibition-mediated inhibition on caspase-3 activity (Figure 4(l))."
USP29 affects BMAL1
| 2
USP29 decreases the amount of BMAL1.
| 1
USP29 decreases the amount of BMAL1. 1 / 1
| 1

reach
"In addition, SIRT1 knockout significantly blocked USP29 inhibition-mediated upregulation on BMAL1 expression in I/R-injured brains (Figures 5(c) and 5(d))."
USP29 deacetylates BMAL1.
| 1
USP29 leads to the deacetylation of BMAL1. 1 / 1
| 1

reach
"Mechanistically, neuronal USP29 enhances p53/miR-34a-mediated SIRT1 downregulation and then promotes the acetylation and suppression of BMAL1, thereby aggravating oxidative stress and apoptosis upon cerebral I/R injury (Figure 7)."
USP29 affects Aldh2
2 |
2 |

No evidence text available

No evidence text available
USP29 affects Acsl5
2 |
2 |

No evidence text available

No evidence text available
USP29 affects Acox1
2 |
2 |

No evidence text available

No evidence text available
TRIM38 affects USP29
| 2
| 1

sparser
"Interestingly, we found that TRIM38 constitutively interacts with cGAS but not with USP29, whereas USP29 constitutively interacts with cGAS but not with TRIM38 (Supplementary information, Fig.  xref ), indicating that TRIM38 and USP29 independently interacts with and regulates cGAS."
TRIM38 binds USP29 and cGAS. 1 / 1
| 1

sparser
"In addition, we found that TRIM38 and USP29 interacts with cGAS independently, suggesting that TRIM38-mediated sumoylation and USP29-mediated deubiquitination of cGAS cooperatively regulate the stability of cGAS."
TP53 affects USP29
1 | 1
1 | 1

reach
"In response to oxidative stress, USP29 binds to and stabilizes p53."

No evidence text available
TNF affects USP29
| 2
TNF inhibits USP29.
| 1
TNF inhibits USP29. 1 / 1
| 1

reach
"Interestingly, the levels of cGAS and the expression of Tnf, Il6, Il12p40 and Ifit3 were significantly decreased in Trex1 -/- Usp29 m/m BMDCs compared to Trex1 -/- BMDCs (Supplementary information, Fig.S7d)."
TNF increases the amount of USP29.
| 1
TNF increases the amount of USP29. 1 / 1
| 1

reach
"19 In addition, the transcription of USP29 in gastric cancer cells could also be induced by TGF‐β, TNFα, and hypoxia, which were reported to be present in the tumor microenvironment and promote EMT and metastasis."
TAK1 inhibitors affects USP29
| 2
TAK1 inhibitors inhibits USP29.
| 1
TAK1 inhibitors inhibits USP29. 1 / 1
| 1

reach
"TAK1 inhibitors eliminated the detrimental effects of USP29 knockout on mouse primary hepatocytes under H/R injury."
TAK1 inhibitors activates USP29.
| 1
TAK1 inhibitors activates USP29. 1 / 1
| 1

reach
"Moreover, TAK1 inhibitors abrogated the protective effect of USP29 on hepatic I/R injury, suggesting that the regulation of hepatic I/R injury by USP29 is dependent on the TAK1-JNK/p38 pathway.We used USP29 full knockout mice rather than hepatocyte-specific knockout mice, which may contain confounding effects of USP29 function in hepatic inflammatory cells."
SIRT1 knockout affects USP29
| 2
SIRT1 knockout inhibits USP29. 2 / 2
| 2

eidos
"And SIRT1 knockout also blocked USP29 inhibition-mediated inhibition on caspase-3 activity ( Figure 4 ( l ) ) ."

eidos
"In addition , SIRT1 knockout significantly blocked USP29 inhibition-mediated upregulation on BMAL1 expression in I / R-injured brains ( Figures 5 ( c ) and 5 ( d ) ) ."
| 2

eidos
"USP29 expression could be induced by oxidative stress , which in turn promoted cell apoptosis [ 25 ] ."

eidos
"In this context , a previous study has shown that oxidative stress induces JTV1-FBP association to co-activate transcription of USP29.39 Whether and how HSV-1 infection promotes oxidative stress to activate transcription of USP29 requires further investigations ."
NFE2L2 affects USP29
2 |
2 |

No evidence text available

No evidence text available
Mice affects USP29
| 2
Mice activates USP29. 2 / 2
| 2

reach
"After being fed a HFHC diet, the USP29-overexpressing mice presented lower body weights and blood glucose levels than those of the controls (Supplementary Fig. 4B, C)."

reach
"Moreover, the liver weights, white adipose weights, and ratio of liver weights or white adipose weights to body weights in USP29-overexpressing mice were markedly lower than those in controls (Supplementary Fig. 4F, G)."
MKI67 affects USP29
| 2
| 1

sparser
"Our results showed that USP29 and Ki67 are not binding each other ( xref ), suggesting that the expression of USP29 increases Ki67 expression in colon cancer cells without interacting with Ki67."
HCT116 binds USP29 and MKI67. 1 / 1
| 1

sparser
"We next checked the interactions between endogenous USP29 and Ki67 in HCT116 cells."
Infections affects USP29
| 2
| 2

eidos
"Our results suggest that HSV-1 infection upregulated USP29 , which might be responsible for increased USP29-cGAS association after HSV-1 infection ."

eidos
"Sequence analysis of USP29 promoter ( -5000 bp ~ +100 bp ) failed to identify any recognizable IRF , NF-kappaB , or STAT binding sites , indicating that the transcriptional regulation of USP29 by viral infection is different from that of OTUD4 , USP25 or INKIT previously reported.37 ,40,41 In this context , it has been shown that transcription factors JTV1 and FBP co-activate USP29 transcription in response to oxidative stress and that one of the evolutionarily conserved sequence elements ( CSE1 ) on Usp29 promoter suppresses its transcription.39 ,42 USP29 promotes HSV-1-triggered innate antiviral signaling in THP-1 cells Because USP29 interacted with cGAS and was upregulated by HSV-1 infection , we investigated its role in regulating HSV-1 - or cytoplasmic DNA-triggered innate immune signaling ."
ELAVL1 affects USP29
1 | 1
1 | 1

No evidence text available

sparser
"Furthermore, RIP-qPCR experiments were conducted to assess the interaction between ELAVL1 and USP29 mRNA, which revealed that ELAVL1 significantly enriched USP29 mRNA compared to the IgG control, indicating a direct interaction between ELAVL1 and USP29 mRNA (Fig. xref )."
CDC25A affects USP29
| 2
| 2

sparser
"To further investigate the physical association of Cdc25A and USP29, HEK293 cells were co-transfected with Myc-Cdc25A and GFP-USP29 and subjected to immunoprecipitation with anti-Myc or -GFP antibodies, followed by Western blotting with reciprocal antibodies."

sparser
"Thus, our data indicated that Cdc25A and USP29 bound to each other and were co-localized."
Aldh2 affects USP29
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No evidence text available

No evidence text available
Acsl5 affects USP29
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2 |

No evidence text available

No evidence text available
Acox1 affects USP29
2 |
2 |

No evidence text available

No evidence text available
1 |
1 |

No evidence text available
Treatment IFNs affects USP29
| 1
Treatment IFNs activates USP29. 1 / 1
| 1

eidos
"Interestingly , SeV infection , transfection of dsDNA or Poly ( I :C ) or treatment of type I IFNs did not induce upregulation of USP29 ."
Transfection dsDNA Poly affects USP29
| 1
Transfection dsDNA Poly activates USP29. 1 / 1
| 1

eidos
"Interestingly , SeV infection , transfection of dsDNA or Poly ( I :C ) or treatment of type I IFNs did not induce upregulation of USP29 ."
1 |
Thioacetamide decreases the amount of USP29. 1 / 1
1 |

No evidence text available
Thimerosal affects USP29
1 |
Thimerosal decreases the amount of USP29. 1 / 1
1 |

No evidence text available
Promoter affects USP29
| 1
Promoter decreases the amount of USP29. 1 / 1
| 1

reach
"Deletion of the U1 promoter results in a loss of allele-specific methylation of Peg3-DMR, causing the biallelic expression or double dosage of Peg3 and Usp29 [15]."
Polyubiquitin affects USP29
| 1
USP29 binds polyubiquitin. 1 / 1
| 1

sparser
"USP29 constitutively interacts with cGAS and deconjugates K48-linked polyubiquitin chains from cGAS in the absence or presence of HSV-1 infection, which stabilizes cGAS and promotes cGAS-mediated cellular antiviral responses."
Polyubiquitin chain affects USP29
| 1
USP29 binds polyubiquitin chain. 1 / 1
| 1

reach
"An USP29 binds and cleaves the polyubiquitin chain from p53 upon oxidative stress, thus coordinating the molecular and cellular stress response from oxidative stress [279]."
Permethrin affects USP29
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Permethrin decreases the amount of USP29. 1 / 1
1 |

No evidence text available
Perfluorohexanesulfonic acid decreases the amount of USP29. 1 / 1
1 |

No evidence text available
Paraquat affects USP29
1 |
Paraquat decreases the amount of USP29. 1 / 1
1 |

No evidence text available
Paclitaxel affects USP29
| 1
Paclitaxel deubiquitinates USP29. 1 / 1
| 1

reach
"Additionally, a rise in USP29 expression enhances resistance of NSCLC cells to Dox and paclitaxel by deubiquitinating Snail1 via USP29 [94]."
Oncoprotein affects USP29
| 1
USP29 binds oncoprotein and HR. 1 / 1
| 1

sparser
"The USP29, a DUB implicated in thiol-dependent hydrolysis, also interacts with HR HPV-16, -18 and -33 E7 oncoproteins [ xref ]."
Nuclear factor-like 2 affects USP29
| 1
USP29 binds nuclear factor-like 2. 1 / 1
| 1

reach
"We then showed that USP29 interacts with, deubiquitinates, and therefore, stabilizes nuclear factor-like 2 (NRF2), thereby regulating microglia/macrophages polarization."
Methylmercury chloride decreases the amount of USP29. 1 / 1
1 |

No evidence text available
Methimazole affects USP29
1 |
Methimazole decreases the amount of USP29. 1 / 1
1 |

No evidence text available
Metastatic-inducing affects USP29
| 1
Metastatic-inducing activates USP29. 1 / 1
| 1

eidos
"USP29 can be induced by major EMT and metastatic-inducing factors such as TGFbeta , TNFalpha , and hypoxia ."
| PMC
Maneb affects USP29
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Maneb decreases the amount of USP29. 1 / 1
1 |

No evidence text available
Lead atom affects USP29
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Lead atom methylates USP29. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-335-5p decreases the amount of USP29. 1 / 1
1 |

No evidence text available
Element binding protein affects USP29
| 1
Element binding protein activates USP29. 1 / 1
| 1

sparser
"Ubiquitin-specific peptidase 29 (USP29) deconjugates ubiquitin from p53 and stabilizes p53. xref USP29 is activated by the far upstream element binding protein (FBP) and reverses MDM2-directed p53 ubiquitination to protect p53 from degradation."
1 |
Deltamethrin increases the amount of USP29. 1 / 1
1 |

No evidence text available
Crocidolite asbestos increases the amount of USP29. 1 / 1
1 |

No evidence text available
CGAS affects USP25
| 1
USP25 binds USP29 and cGAS. 1 / 1
| 1

sparser
"To test this hypothesis, we conducted an unbiased screening of cGAS-interacting DUBs by cotransfection and immunoprecipitation assays as previous established, xref – xref and found that USP25 and USP29 interacted with cGAS in such a system (Fig.  xref )."
CGAS affects TRIM38
| 1
TRIM38 binds USP29 and cGAS. 1 / 1
| 1

sparser
"In addition, we found that TRIM38 and USP29 interacts with cGAS independently, suggesting that TRIM38-mediated sumoylation and USP29-mediated deubiquitination of cGAS cooperatively regulate the stability of cGAS."
1 |
Benzo[a]pyrene increases the amount of USP29. 1 / 1
1 |

No evidence text available
Amitrole affects USP29
1 |
Amitrole decreases the amount of USP29. 1 / 1
1 |

No evidence text available
Agarose affects USP29
| 1
| 1

reach
"Bacterial‐expressed GST and GST‐USP29 protein bound to Pierce Glutathione Agarose was incubated with PET28A‐TWIST1, purified from the Escherichia coli strain BL21 overnight at 4 °C."
USP33 affects USP29
| 1
USP29 binds USP33 and E7. 1 / 1
| 1

sparser
"The Gaussia princeps luciferase protein complementation assay shows that USP15 interacts with the key oncoprotein human papillomavirus (HPV) E6, while USP29 and USP33 bind to E7 protein, suggesting that cellular DUBs impact HPV tumorigenesis by regulating the stability of the viral proteins [ xref ]."
1 |
1 |

No evidence text available
USP29 affects virulence
| 1
| 1

reach
"In addition, USP29 inhibits NF-κB activation and subsequent IFN-1 production during SARS-CoV-2 virus infection by deubiquitinating ORF9b, thereby enhancing the pathogen’s virulence (32)."
| 1
| 1

reach
"To examine whether USP29-mediated stabilization of ORF9b affects viral infection, we first examined the effect of USP29 on VSV-eGFP replication in the presence of ORF9b."
USP29 affects ubiquitination cGAS K464R cGAS
| 1
USP29 inhibits ubiquitination cGAS K464R cGAS. 1 / 1
| 1

eidos
"In this context , we noted that USP29 inhibited ubiquitination of cGAS ( K464R ) but not cGAS ( K271R ) after HSV-1 infection , and that knockout of USP29 potentiated ubiquitination of cGAS ( K464R ) but not cGAS ( K271R ) before or after HSV-1 infection ."
USP29 affects type I IFNs
| 1
USP29 activates type I IFNs. 1 / 1
| 1

reach
"Consistently, Usp29 m/m mice produce decreased type I IFNs and proinflammatory cytokines after HSV-1 infection and are hypersensitive to HSV-1 infection compared to the wild-type littermates."
USP29 affects tumorigenesis in vitro
| 1
USP29 activates tumorigenesis in vitro. 1 / 1
| 1

eidos
"Altogether , our results show that USP29 is highly upregulated in colon cancers and promotes tumorigenesis in vitro and in vivo ."
USP29 affects tumor mouse xenograft models
| 1
USP29 activates tumor mouse xenograft models. 1 / 1
| 1

eidos
"Furthermore , our data suggests that USP29 knockdown reduced the tumor volume of mouse xenograft models ."
USP29 affects sub-G1 population
| 1
USP29 inhibits sub-G1 population. 1 / 1
| 1

eidos
"Consistent with those results , loss of USP29 elevated the sub-G1 population and cleaved caspase-3 expression ( Figure 4 ) ."

reach
"We also found that USP29 overexpression in hepatocytes significantly reduced the expression levels of inflammatory factors (Tnf, Il6, Il8 and Ccl2) and inhibited the activation of the NF-κB signaling pathway (Figures 2C, D)."

reach
"In addition, USP29 overexpression induced by chemotherapy and oxidative stress treatment can promote chemotherapy resistance by hijacking the USP29/Snail1 axis, which is associated with enhanced cancer stem cell properties, poor prognosis and drug resistance in lung cancer (91)."

reach
"USP29 enhances chemotherapy-induced stemness in non-small cell lung cancer via stabilizing Snail1 in response to oxidative stress."

reach
"Indeed, our findings, along with previous reports that depletion of USP29 caused cell cycle arrest and apoptosis in cervical cancer [25], colorectal cancer [38], and osteosarcoma cell [41]."
USP29 affects purine
| 1
USP29 activates purine. 1 / 1
| 1

reach
"Despite this, USP29 depletion significantly reduced the enrichment of M5 purine intermediates, including AMP, ADP/dGDP, ATP, GDP and GTP, either labeled for 1 or 24 h (Figure 2c)."

reach
"Here we found that USP29 rescued CDH1-mediated decrease of AURKB and proteasome inhibitor MG132 treatment also rescued the AURKB downregulation induced by USP29 depletion (Fig. 3D and E)."
USP29 affects proteasomal cGAS
| 1
USP29 inhibits proteasomal cGAS. 1 / 1
| 1

eidos
"These data together suggest that USP29 deconjugates K48-linked polyubiquitin chains from cGAS and thereby inhibits the proteasomal degradation of cGAS ."
USP29 affects proliferative behavior colon cancer cells
| 1
USP29 activates proliferative behavior colon cancer cells. 1 / 1
| 1

eidos
"Generation of USP29-Depleted Cells Using CRISPR-Cas9 System Given that USP29 promotes the proliferative behavior of colon cancer cells , we examined its role in the canonical DNA damage and cell-cycle progression of HCT116 cells by generating USP29 knockout using the CRISPR-Cas9 system ."
USP29 affects polyubiquitination protein
| 1
USP29 inhibits polyubiquitination protein. 1 / 1
| 1

eidos
"Subsequent sub-screening identified 23 DUBs that interact with SNAI1 , out of which USP29 , USP36 , and USP37 upregulate and reduce polyubiquitination of the SNAI1 protein ."
| PMC
USP29 affects polyubiquitin
| 1
USP29 binds polyubiquitin. 1 / 1
| 1

sparser
"USP29 constitutively interacts with cGAS and deconjugates K48-linked polyubiquitin chains from cGAS in the absence or presence of HSV-1 infection, which stabilizes cGAS and promotes cGAS-mediated cellular antiviral responses."
USP29 affects polyubiquitin chain
| 1
USP29 binds polyubiquitin chain. 1 / 1
| 1

reach
"An USP29 binds and cleaves the polyubiquitin chain from p53 upon oxidative stress, thus coordinating the molecular and cellular stress response from oxidative stress [279]."
| 1
| 1

reach
"We observed upregulation of USP29 protein levels in both transverse aortic constriction-induced hypertrophic hearts (upregulated by 159.8%) and phenylephrine-induced hypertrophic cardiomyocytes (upregulated by 184.6%)."
USP29 affects oncoprotein
| 1
USP29 binds oncoprotein and HR. 1 / 1
| 1

sparser
"The USP29, a DUB implicated in thiol-dependent hydrolysis, also interacts with HR HPV-16, -18 and -33 E7 oncoproteins [ xref ]."
USP29 affects nuclear factor-like 2
| 1
USP29 binds nuclear factor-like 2. 1 / 1
| 1

reach
"We then showed that USP29 interacts with, deubiquitinates, and therefore, stabilizes nuclear factor-like 2 (NRF2), thereby regulating microglia/macrophages polarization."

reach
"In contrast, depletion of endogenous USP29 by specific sgRNAs or shRNA caused a remarkable downregulation of cell proliferation (Fig. 1D and E, Supplementary Fig. 1C and 1E)."
USP29 affects interaction Snail SCP1
| 1
USP29 activates interaction Snail SCP1. 1 / 1
| 1

eidos
"Mechanistically , USP29 enhances the interaction of Snail and SCP1 , resulting in simultaneous dephosphorylation and deubiquitination of Snail and thereafter cooperative prevention of Snail degradation ."
USP29 affects innate antiviral responses
| 1
USP29 activates innate antiviral responses. 1 / 1
| 1

eidos
"In addition , USP29 also interacted with cyclic GMP-AMP synthase and promoted innate antiviral responses against DNA viruses and autoimmunity [ 24 ] ."
| 1

reach
"Analysis of RNA sequencing data in SK-N-BE2 cells demonstrated that USP29 promoted the gene expression of metabolic enzymes involved in nucleotide anabolism, probably by regulating MYC and E2F downstream pathways."
USP29 affects focus 53BP1
| 1
USP29 activates focus 53BP1. 1 / 1
| 1

eidos
"Importantly , USP29 knockdown inhibits the irradiation-induced increase in H4K20 monomethylation , thereby preventing focus formation of 53BP1 in response to DNA damage ."
USP29 affects downstream targets
| 1
USP29 activates downstream targets. 1 / 1
| 1

eidos
"In view of the fact that USP29 is a deubiquitinase and modulates the downstream targets via catalyzing the removal of monoubiquitin or polyubiquitin chains at the posttranscriptional level , therefore , we speculated that SIRT1 expression was not directly modulated by USP29 ."
USP29 affects dGDP
| 1
USP29 activates dGDP. 1 / 1
| 1

reach
"Despite this, USP29 depletion significantly reduced the enrichment of M5 purine intermediates, including AMP, ADP/dGDP, ATP, GDP and GTP, either labeled for 1 or 24 h (Figure 2c)."
USP29 affects cleaved caspase-3
| 1
USP29 inhibits cleaved caspase-3. 1 / 1
| 1

eidos
"We found that USP29 depletion increased the expression of cleaved caspase-3 compared with the scrambled sgRNA control ( p = 0.0007 ) ( Figure 4E ) , and USP29 reconstitution decreased the expression of cleaved caspase-3 compared with the USP29-depleted cells ( p = 0.0025 ) ."
USP29 affects cerebral R-induced caspase-3 activation
| 1
USP29 activates cerebral R-induced caspase-3 activation. 1 / 1
| 1

eidos
"And USP29 inhibition also suppressed cerebral I / R-induced caspase-3 activation ( Figure 2 ( l ) ) ."
USP29 affects cerebral R-induced SIRT1
| 1
USP29 activates cerebral R-induced SIRT1. 1 / 1
| 1

eidos
"As shown in Figures 4 ( a ) -4 ( d ) , USP29 inhibition did not affect cerebral SIRT3 expression upon I / R injury but restored cerebral I / R-induced downregulation of SIRT1 , while USP29 overexpression further decreased SIRT1 expression in I / R-injured brains ."
USP29 affects cerebral R-caused brain edema
| 1
USP29 activates cerebral R-caused brain edema. 1 / 1
| 1

eidos
"In addition , USP29 inhibition significantly attenuated cerebral I / R-caused brain edema , as evidenced by the decreased brain water content ( Figure 1 ( i ) ) ."
USP29 affects cerebral R injury mice
| 1
USP29 activates cerebral R injury mice. 1 / 1
| 1

eidos
"The findings enable us to believe that USP29 inhibition prevents cerebral I / R injury in mice ."
USP29 affects cellular sensitivity irradiation
| 1
USP29 inhibits cellular sensitivity irradiation. 1 / 1
| 1

eidos
"Lastly , depletion of USP29 increases the cellular sensitivity to irradiation ."
USP29 affects cell
| 1
USP29 activates cell. 1 / 1
| 1

eidos
"USP29 knockdown significantly decreased CRC cell proliferation in vitro ."
USP29 affects cell division cycle
| 1
USP29 activates cell division cycle. 1 / 1
| 1

reach
"17 In a cervical cancer study, the overexpression of USP29 enables the stabilization of the cell division cycle 25A (Cdc25A) and enhances the oncogenic potential of HeLa cells."
USP29 affects cell cycle
| 1
| 1

reach
"Considering the important role of AURKB in controlling cell cycle, we reasoned that USP29 may promote gastric cancer cell proliferation by regulating cell cycle progression."
USP29 affects cell apoptosis
| 1
USP29 activates cell apoptosis. 1 / 1
| 1

eidos
"Liu et al. reported that USP29 expression was elevated upon oxidative stress and then promoted cell apoptosis via directly deubiquitinating p53 [ 25 ] ."
USP29 affects benefits
| 1
USP29 activates benefits. 1 / 1
| 1

eidos
"Proteomics analysis revealed that ubiquitin-specific protease 29 ( USP29 ) was markedly increased in MEVs , and knockdown of USP29 in MEVs ( shUSP29-MEVs ) abolished MEVs-mediated benefits in vitro and in vivo ."
USP29 affects autoimmunity Cyclic GMP-AMP synthase
| 1
USP29 activates autoimmunity Cyclic GMP-AMP synthase. 1 / 1
| 1

eidos
"USP29 maintains the stability of cGAS and promotes cellular antiviral responses and autoimmunity Cyclic GMP-AMP synthase ( cGAS ) is an essential sensor of cytosolic DNA and critically mediates innate immune responses and autoimmunity ."
USP29 affects autoimmunity Correction
| 1
USP29 activates autoimmunity Correction. 1 / 1
| 1

eidos
"Author Correction : USP29 maintains the stability of cGAS and promotes cellular antiviral responses and autoimmunity Correction to : Cell Research 10.1038 / s41422-020-0341-6 , published online 26 May 2020 We just found several mistakes and typos in the paper published on May 26 , 2020 ."
USP29 affects apoptosis HCT116 cells
| 1
USP29 inhibits apoptosis HCT116 cells. 1 / 1
| 1

eidos
"We also demonstrated that USP29 depletion hampers the colony formation and increases apoptosis of HCT116 cells ."
USP29 affects agarose
| 1
| 1

reach
"Bacterial‐expressed GST and GST‐USP29 protein bound to Pierce Glutathione Agarose was incubated with PET28A‐TWIST1, purified from the Escherichia coli strain BL21 overnight at 4 °C."
USP29 affects Zim1
| 1
USP29 increases the amount of Zim1. 1 / 1
| 1

reach
"For the genes at the Peg3 imprinted region, decreased expression of Zim1 and increased expression of Usp29, Peg3, and Peg3os were similarly observed comparing female M−Z− embryos with female M+Z+ embryos or comparing male M−Z− embryos with male M+Z+ embryos (Table 2, Supplementary Figure S10A-S10A')."
USP29 affects USP33
| 1
USP29 binds USP33 and E7. 1 / 1
| 1

sparser
"The Gaussia princeps luciferase protein complementation assay shows that USP15 interacts with the key oncoprotein human papillomavirus (HPV) E6, while USP29 and USP33 bind to E7 protein, suggesting that cellular DUBs impact HPV tumorigenesis by regulating the stability of the viral proteins [ xref ]."
USP29 affects USP25
| 1
USP25 binds USP29 and cGAS. 1 / 1
| 1

sparser
"To test this hypothesis, we conducted an unbiased screening of cGAS-interacting DUBs by cotransfection and immunoprecipitation assays as previous established, xref – xref and found that USP25 and USP29 interacted with cGAS in such a system (Fig.  xref )."
USP29 affects Thymoma
| 1
USP29 activates Thymoma. 1 / 1
| 1

reach
"In MG-related thymomas, the mRNA and protein levels of deubiquitylases USP29 were substantially elevated."
USP29 affects Thr578
| 1
CDK1 binds USP29 and Thr578. 1 / 1
| 1

reach
"First, we identify that CDK1 binds and phosphorylates USP29 at Ser575, Thr578, and Ser672, while the phosphorylation deficient 3A mutation almost abolishes the phosphorylation of USP29 (Figure 4 and Figure S4, Supporting Information)."
USP29 affects TXN
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1 |

No evidence text available
USP29 affects TP53BP1
| 1
| 1

reach
"To study the functional consequences of regulation of SETD8 by USP29, we analyzed the effect of USP29 depletion on the DNA damage-induced focus formation of 53BP1 by immunofluorescence (IF) (Figure 4A)."
USP29 affects SYCP1
| 1
| 1

reach
"Collectively, these data indicate that Snail is a crucial substrate for USP29 to promote cell migration and USP29 and SCP1 complex may be new therapeutic targets to treat metastatic cancer."
USP29 affects SOD2
| 1
USP29 decreases the amount of SOD2. 1 / 1
| 1

reach
"Accordingly, USP29 knockout also restored the expressions of downstream HO-1 and SOD2 (Figure 2(g))."
USP29 affects SETD8 protein
| 1
USP29 activates SETD8 protein. 1 / 1
| 1

eidos
"Depletion of USP29 leads to decreased SETD8 protein levels , an effect that is independent of the cell cycle ."
USP29 affects RPS27L
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1 |

No evidence text available
USP29 affects RPS13
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1 |

No evidence text available
USP29 affects ROR
| 1
USP29 increases the amount of ROR. 1 / 1
| 1

reach
"This work exhibited that the elevated USP29 enhanced RORγt expression, which in turn affected the Th17 cell growth in thymomatous MG."
USP29 affects RFWD3
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1 |

No evidence text available
USP29 affects PSMA3
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1 |

No evidence text available
USP29 affects PCNA
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1 |

No evidence text available
USP29 affects OGD R-induced caspase-3
| 1
USP29 activates OGD R-induced caspase-3. 1 / 1
| 1

eidos
"We also found that USP29 knockdown significantly suppressed OGD / R-induced increases of caspase-3 activity ( Figure S1C ) ."
USP29 affects Nuclear erythroid 2-related factor 2
| 1
USP29 deubiquitinates Nuclear erythroid 2-related factor 2. 1 / 1
| 1

reach
"For example, USP29 acts as a traumatic spinal cord injury protector by deubiquitinating NRF2 (Nuclear erythroid 2-related factor 2), thereby stabilizing NRF2 function and regulating microglia/macrophage polarization (9)."
| 1
| 1

reach
"USP29 has also been shown to promote neuroblastoma progression by upregulating glycolysis and glutamine catabolism (Kang et al., 2023)."

reach
"Collectively, these data demonstrate a crucial role of USP29 in promoting CSC features through TWIST1.Emerging evidence suggests that EMT and CSCs critically contribute to cancer metastasis and resistance to chemotherapy."

reach
"19 In addition, the transcription of USP29 in gastric cancer cells could also be induced by TGF‐β, TNFα, and hypoxia, which were reported to be present in the tumor microenvironment and promote EMT and metastasis."
USP29 affects NFkappaB
| 1
| 1

reach
"USP29 enhances ORF9b-mediated type I IFN and NF-kappaB inhibition.."
USP29 affects NF-kappaB activation
| 1
USP29 inhibits NF-kappaB activation. 1 / 1
| 1

eidos
"USP29 interacts with the carboxyl end of ORF9b and removes ubiquitin chains from the protein , thereby inhibiting type I interferon ( IFN ) induction and NF-kappaB activation ."
USP29 affects MAVS
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1 |

No evidence text available
USP29 affects MASLD
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USP29 activates MASLD. 1 / 1
| 1

reach
"Next, to test whether USP29 modulates MASLD via ACSL5, the effects of USP29 on ACSL5 were assessed."
USP29 affects MASH
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USP29 activates MASH. 1 / 1
| 1

reach
"Together, these data suggest that USP29 overexpression ameliorates HFHC-induced MASH in mice."
USP29 affects MAP3K5
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1 |

No evidence text available
USP29 affects Lymphoma
| 1
| 1

reach
"Knockout of USP29 depletes MYC and markedly prolongs survival of lymphoma xenografts [ 45 ]."
USP29 affects Ki67 colon cancer cells
| 1
USP29 activates Ki67 colon cancer cells. 1 / 1
| 1

eidos
"Our results showed that USP29 and Ki67 are not binding each other ( Figure S1 ) , suggesting that the expression of USP29 increases Ki67 expression in colon cancer cells without interacting with Ki67 ."
USP29 affects KIF11
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1 |

No evidence text available
USP29 affects K48-linked ubiquitination degradation cGAS
| 1
USP29 inhibits K48-linked ubiquitination degradation cGAS. 1 / 1
| 1

eidos
"Consistent with the notion that USP29 deficiency promoted K48-linked ubiquitination and degradation of cGAS , we found that treatment of MG132 but not 3MA fully prevented the degradation of cGAS in Usp29m / m MEFs in the presence or absence of HSV-1 infection ( Fig. 6g ) ."
USP29 affects IL1B
| 1
USP29 decreases the amount of IL1B. 1 / 1
| 1

reach
"In our study, USP29 overexpression attenuated CD11b-positive inflammatory cells infiltration and downregulated expression of inflammatory factors Tnf and Il1b and pro-apoptotic factors Bax and Bad."
USP29 affects IFNs
| 1
USP29 activates IFNs. 1 / 1
| 1

reach
"Hence, USP27X and USP29 promote the production of cGAMP and downstream IFNs to mediate inflammation and autoimmune responses (Guo et al., 2019; Zhang et al., 2020b)."
USP29 affects IFNA
| 1
USP29 activates IFNA. 1 / 1
| 1

reach
"In addition, knockdown of USP29 significantly impaired the production of IFN-beta, IFN-alpha, IL-6, and TNF after infection of HSV-1 or transfection of various DNA ligands."
USP29 affects IFN-beta IFN-alpha IL-6 TNF
| 1
USP29 activates IFN-beta IFN-alpha IL-6 TNF. 1 / 1
| 1

eidos
"In addition , knockdown of USP29 significantly impaired the production of IFN-beta , IFN-alpha , IL-6 , and TNF after infection of HSV-1 or transfection of various DNA ligands ( Fig. 2e ) ."
USP29 affects IFN-1
| 1
USP29 inhibits IFN-1. 1 / 1
| 1

reach
"In addition, USP29 inhibits NF-κB activation and subsequent IFN-1 production during SARS-CoV-2 virus infection by deubiquitinating ORF9b, thereby enhancing the pathogen’s virulence (32)."
USP29 affects Hypertrophy
| 1
| 1

reach
"Moreover, genetic knockout of USP29 in mice exacerbated transverse aortic constriction-induced heart hypertrophy, dysfunction, and fibrosis, whereas overexpression of USP29 in cardiomyocytes using adeno-associated virus 9 effectively attenuated the hypertrophic response."
USP29 affects HSV-1-triggered downstream
| 1
USP29 activates HSV-1-triggered downstream. 1 / 1
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eidos
"USP29 deficiency impairs HSV-1-triggered expression of downstream genes ."
USP29 affects HSV-1-triggered downstream investigate USP29 regulating antiviral signaling
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USP29 activates HSV-1-triggered downstream investigate USP29 regulating antiviral signaling. 1 / 1
| 1

eidos
"USP29 deficiency impairs HSV-1-triggered expression of downstream genes To further investigate the role of USP29 in regulating antiviral signaling in vivo , we generated USP29-deficient mice by CRISPR / Cas9-mediated genome editing ( Supplementary information , Fig. S3a ) ."
USP29 affects HSPA1B
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1 |

No evidence text available
USP29 affects HRAS
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1 |

No evidence text available
USP29 affects HR
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USP29 binds oncoprotein and HR. 1 / 1
| 1

sparser
"The USP29, a DUB implicated in thiol-dependent hydrolysis, also interacts with HR HPV-16, -18 and -33 E7 oncoproteins [ xref ]."
USP29 affects HMOX1
| 1
USP29 decreases the amount of HMOX1. 1 / 1
| 1

reach
"Accordingly, USP29 knockout also restored the expressions of downstream HO-1 and SOD2 (Figure 2(g))."
USP29 affects HCT116
| 1
HCT116 binds USP29 and MKI67. 1 / 1
| 1

sparser
"We next checked the interactions between endogenous USP29 and Ki67 in HCT116 cells."
USP29 affects H4K20me1
| 1
USP29 decreases the amount of H4K20me1. 1 / 1
| 1

reach
"Interestingly however, treating the cells with IR led to a significant increase in H4K20me1 levels, which was inhibited by the downregulation of USP29 (Figure 5A,B)."
USP29 affects H4K20
| 1
USP29 leads to the methylation of H4K20. 1 / 1
| 1

reach
"USP29 Controls IR-Induced H4K20 Monomethylation and Cell Survival."
USP29 affects H4K20 monomethylation
| 1
USP29 activates H4K20 monomethylation. 1 / 1
| 1

eidos
"Importantly , USP29 knockdown inhibits the irradiation-induced increase in H4K20 monomethylation , thereby preventing focus formation of 53BP1 in response to DNA damage ."
USP29 affects H2AX
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USP29 increases the amount of H2AX. 1 / 1
| 1

sparser
"It is noteworthy that sgRNA1 targeting USP29 induced more γH2AX protein expression than either sgRNA2 or the scrambled sgRNA control ( xref A, lane 2)."
USP29 affects H2AC20
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USP29 deubiquitinates H2AC20. 1 / 1
1 |

"Both USP44 and <span class="match term0">USP29</span> promoted efficient deubiquitylation of histone <span class="match term1">H2A</span>"
USP29 affects Guo
| 1
USP29 activates Guo. 1 / 1
| 1

reach
"Hence, USP27X and USP29 promote the production of cGAMP and downstream IFNs to mediate inflammation and autoimmune responses (Guo et al., 2019; Zhang et al., 2020b)."
USP29 affects GTP
| 1
USP29 activates GTP. 1 / 1
| 1

reach
"Despite this, USP29 depletion significantly reduced the enrichment of M5 purine intermediates, including AMP, ADP/dGDP, ATP, GDP and GTP, either labeled for 1 or 24 h (Figure 2c)."
USP29 affects GOT
| 1
USP29 decreases the amount of GOT. 1 / 1
| 1

reach
"Similarly, the increased white adipose weights, white adipose weight-to-body weight ratios and serum ALT and AST levels induced by USP29 deletion were also reversed by ACSL5 overexpression (Supplementary Fig. 7E, F)."
USP29 affects GDP
| 1
USP29 activates GDP. 1 / 1
| 1

reach
"Despite this, USP29 depletion significantly reduced the enrichment of M5 purine intermediates, including AMP, ADP/dGDP, ATP, GDP and GTP, either labeled for 1 or 24 h (Figure 2c)."
USP29 affects Fig. S4A-B
| 1
USP29 increases the amount of Fig. S4A-B. 1 / 1
| 1

reach
"Accordingly, USP29 WT but not C294A increased the expression of ORF9b (Fig. S4A-B)."
USP29 affects FBP1
| 1
USP29 increases the amount of FBP1. 1 / 1
| 1

reach
"JTV1 co-activates FBP to induce USP29 transcription and stabilize p53 in response to oxidative stress."
USP29 affects FAO-related
| 1
USP29 decreases the amount of FAO-related. 1 / 1
| 1

reach
"The expression of FAO-related (Cpt1a and Ehhadh) and inflammation-related (Cxcl2 and Ccl5) genes induced by USP29 knockout was significantly reversed by ACSL5 overexpression (Fig. 6D)."
USP29 affects EPAS1
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1 |

No evidence text available
USP29 affects ELANE
| 1
USP29 activates ELANE. 1 / 1
| 1

reach
"Fig. 3d), supporting a role of USP29 in stabilizing HIF1α in HCC and HEK cells.We further determined the robustness of USP29-mediated stabilization of HIF1α in a hypoxia-reoxygenation assay in Sorafenib-resistant HLE cells."
USP29 affects Disease
| 1
USP29 activates Disease. 1 / 1
| 1

eidos
"Recently , USP29 was found to be abundantly expressed in the brain and contributed to the progression of Parkinson 's disease [ 26 ] ."

reach
"Moreover, USP29 promoted the virulence of VSV-eGFP and transcription and replication-competent SARS-CoV-2 virus-like particles (trVLP) by enhancing ORF9b-mediated inhibition of IFN induction and NF-κB activation."
USP29 affects DNA Delays Cell-Cycle Progression investigate
| 1
USP29 activates DNA Delays Cell-Cycle Progression investigate. 1 / 1
| 1

eidos
"USP29 Depletion Induces DNA Damage and Delays Cell-Cycle Progression To investigate whether the loss of USP29 function in HCT116 causes DNA damage , we checked the expression of a canonical DNA double strand break marker , gammaH2AX ."
USP29 affects Colon Cancer Growth
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USP29 activates Colon Cancer Growth. 1 / 1
| 1

eidos
"Loss of USP29 Expression Inhibits Colon Cancer Growth In Vitro and In Vivo Because USP29 facilitates colon cancer proliferation , we wondered whether USP29-depleted HCT116 cells might inhibit colon cancer growth in vitro and in vivo ."
USP29 affects Collagen
| 1
| 1

reach
"Intriguingly, USP29 overexpression obviously attenuated hepatic steatosis, inflammation, collagen deposition and liver injury (Supplementary Fig. 4H–K)."
USP29 affects Cerebral R Injury
| 1
USP29 activates Cerebral R Injury. 1 / 1
| 1

eidos
"USP29 Inhibition Prevents Cerebral I / R Injury in Mice We first examined the expression of USP29 in the primary cortical neurons upon OGD / R stimulation and found that the protein and mRNA of USP29 were increased in OGD / R-challenged cells ( Figures 1 ( a ) and 1 ( b ) ) ."
USP29 affects Cerebral Oxidative Stress Apoptosis free radicals contribute cerebral R injury neuronal apoptosis
| 1
USP29 activates Cerebral Oxidative Stress Apoptosis free radicals contribute cerebral R injury neuronal apoptosis. 1 / 1
| 1

eidos
"USP29 Inhibition Diminishes Cerebral I / R-Induced Oxidative Stress and Apoptosis Excessive free radicals contribute to the progression of cerebral I / R injury and neuronal apoptosis ; therefore , we next measured the role of USP29 inhibition on oxidative stress ."
USP29 affects Cell Previous researchers reported functions USP29 regulate cancer-related proteins
| 1
USP29 activates Cell Previous researchers reported functions USP29 regulate cancer-related proteins. 1 / 1
| 1

eidos
"USP29 Is Highly Upregulated in Clinical Human Colon Cancer Tissues and Promotes Cell Proliferation Previous researchers reported the functions of USP29 and its ability to regulate cancer-related proteins [ 12,13,14 ] ."
| 1
| 1

reach
"USP29 attenuates H/R-stimulated hepatocyte inflammation and apoptosis."

reach
"USP29 enhances chemotherapy-induced stemness in non-small cell lung cancer via stabilizing Snail1 in response to oxidative stress."
USP29 affects Cadherin
| 1
| 1

reach
"Since USP29 regulates the stability of TWIST1 and consequently decreases the expression of E‐cadherin, we hypothesized that USP29 could be critical for the migration and invasion of TNBC cells in vitro."
USP29 affects CTSB
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1 |

No evidence text available
USP29 affects CTNNB1
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1 |

No evidence text available
USP29 affects CTDSPL
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1 |

No evidence text available
USP29 affects CTDSP2
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1 |

No evidence text available
USP29 affects CHEK1
| 1
USP29 phosphorylates CHEK1. 1 / 1
| 1

reach
"Most importantly, USP29 knockdown results in an impaired phosphorylation of Chk1 after DNA damage and USP29 depleted cells show a major defect in the S-phase progression."
USP29 affects CEACAM6
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1 |

No evidence text available
USP29 affects CDH1
| 1
USP29 inhibits CDH1. 1 / 1
| 1

reach
"Here we found that USP29 rescued CDH1-mediated decrease of AURKB and proteasome inhibitor MG132 treatment also rescued the AURKB downregulation induced by USP29 depletion (Fig. 3D and E)."
USP29 affects CCAR2
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1 |

No evidence text available
USP29 affects CASP14
| 1
USP29 activates CASP14. 1 / 1
| 1

reach
"USP29 Inhibition Prevents Cerebral I/R Injury in Mice."
USP29 affects Brain Edema
| 1
| 1

reach
"In addition, USP29 inhibition significantly attenuated cerebral I/R-caused brain edema, as evidenced by the decreased brain water content (Figure 1(i))."
USP29 affects BCL2
| 1
USP29 increases the amount of BCL2. 1 / 1
| 1

reach
"In addition, after the H/R challenge, USP29 overexpression in hepatocytes suppressed the expression of pro-apoptotic-related molecules (Bad, Bax, and C-caspase 3) and promoted the expression of Bcl-2 when compared to those expressions in the control cells (Figures 2E, F)."
USP29 affects BBC3
| 1
USP29 increases the amount of BBC3. 1 / 1
| 1

reach
"Under oxidative stress, USP29 is induced by JTV1 and FBP, and stabilizes p53 and induces PUMA transcription and apoptosis [21] ."
USP29 affects BAX
| 1
USP29 inhibits BAX. 1 / 1
| 1

reach
"In our study, USP29 overexpression attenuated CD11b-positive inflammatory cells infiltration and downregulated expression of inflammatory factors Tnf and Il1b and pro-apoptotic factors Bax and Bad."
USP29 affects BAD
| 1
USP29 inhibits BAD. 1 / 1
| 1

reach
"In our study, USP29 overexpression attenuated CD11b-positive inflammatory cells infiltration and downregulated expression of inflammatory factors Tnf and Il1b and pro-apoptotic factors Bax and Bad."
| 1
| 1

eidos
"USP29 deficiency alleviates autoimmunity in Trex1 - / - mice Consistent with the results obtained with USP29 knockout cells , we found that the protein levels of cGAS were substantially lower in various organs such as heart , liver , kidney and spleen from Usp29m / m mice than in those from Usp29 + / + mice ( Fig. 7a ) ."
USP29 affects ATP
| 1
USP29 activates ATP. 1 / 1
| 1

reach
"Despite this, USP29 depletion significantly reduced the enrichment of M5 purine intermediates, including AMP, ADP/dGDP, ATP, GDP and GTP, either labeled for 1 or 24 h (Figure 2c)."
USP29 affects ARNT
| 1
USP29 leads to the deacetylation of ARNT. 1 / 1
| 1

reach
"Mechanistically, neuronal USP29 enhanced p53/miR-34a-mediated silent information regulator 1 downregulation and then promoted the acetylation and suppression of brain and muscle ARNT-like protein, thereby aggravating oxidative stress and apoptosis upon cerebral I/R injury."
USP29 affects ADP
| 1
USP29 activates ADP. 1 / 1
| 1

reach
"Despite this, USP29 depletion significantly reduced the enrichment of M5 purine intermediates, including AMP, ADP/dGDP, ATP, GDP and GTP, either labeled for 1 or 24 h (Figure 2c)."

reach
"Despite this, USP29 depletion significantly reduced the enrichment of M5 purine intermediates, including AMP, ADP/dGDP, ATP, GDP and GTP, either labeled for 1 or 24 h (Figure 2c)."
USP25 affects cGAS
| 1
USP25 binds USP29 and cGAS. 1 / 1
| 1

sparser
"To test this hypothesis, we conducted an unbiased screening of cGAS-interacting DUBs by cotransfection and immunoprecipitation assays as previous established, xref – xref and found that USP25 and USP29 interacted with cGAS in such a system (Fig.  xref )."
Thr578 affects USP29
| 1
CDK1 binds USP29 and Thr578. 1 / 1
| 1

reach
"First, we identify that CDK1 binds and phosphorylates USP29 at Ser575, Thr578, and Ser672, while the phosphorylation deficient 3A mutation almost abolishes the phosphorylation of USP29 (Figure 4 and Figure S4, Supporting Information)."
TXN affects USP29
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1 |

No evidence text available
TRIM38 affects cGAS
| 1
TRIM38 binds USP29 and cGAS. 1 / 1
| 1

sparser
"In addition, we found that TRIM38 and USP29 interacts with cGAS independently, suggesting that TRIM38-mediated sumoylation and USP29-mediated deubiquitination of cGAS cooperatively regulate the stability of cGAS."
TGF‐β affects USP29
| 1
TGF‐β increases the amount of USP29. 1 / 1
| 1

reach
"19 In addition, the transcription of USP29 in gastric cancer cells could also be induced by TGF‐β, TNFα, and hypoxia, which were reported to be present in the tumor microenvironment and promote EMT and metastasis."
Suppl affects USP29
| 1
Suppl inhibits USP29. 1 / 1
| 1

reach
"First, analysis of HLE cells by immunofluorescence microscopy revealed an increase in nuclear localization of HIF1α upon expression of Myc-tagged USP29 (Fig. 2e) and a reduction of nuclear HIF1α upon siRNA-mediated depletion of USP29 (Fig. 2f; Suppl."
SeV infection affects USP29
| 1
SeV infection activates USP29. 1 / 1
| 1

eidos
"Interestingly , SeV infection , transfection of dsDNA or Poly ( I :C ) or treatment of type I IFNs did not induce upregulation of USP29 ."
SYCP1 affects USP29
| 1
| 1

reach
"Collectively, these data indicate that Snail is a crucial substrate for USP29 to promote cell migration and USP29 and SCP1 complex may be new therapeutic targets to treat metastatic cancer."
SOX8 affects USP29
| 1
SOX8 inhibits USP29. 1 / 1
| 1

reach
"Moreover, overexpression of KIAA1429 or SOX8 reversed the inhibitory effects of USP29 depletion on CRC cell proliferation."
SIRT1 silence affects USP29
| 1
SIRT1 silence inhibits USP29. 1 / 1
| 1

eidos
"Accordingly , SIRT1 silence blocked USP29 inhibition-mediated inhibition on caspase-3 activity in OGD / R-stimulated neurons ( Figure S2C ) ."
RPS27L affects USP29
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No evidence text available
RPS13 affects USP29
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1 |

No evidence text available
RFWD3 affects USP29
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1 |

No evidence text available
Plastics affects USP29
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Plastics increases the amount of USP29. 1 / 1
1 |

No evidence text available
Peg3-DMR affects USP29
| 1
Peg3-DMR activates USP29. 1 / 1
| 1

reach
"This was expected since the deletion of the Peg3-DMR in the KO2 samples abolishes the bidirectional promoter for Peg3/Usp29."
PSMA3 affects USP29
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1 |

No evidence text available
PFT-α affects USP29
| 1
PFT-α decreases the amount of USP29. 1 / 1
| 1

reach
"And the pretreatment with PFT-α or miR-34a antagomir also significantly blocked the deleterious effects of USP29 overexpression upon cerebral I/R injury, as evidenced by the decreased infarct volume, neurological deficit score, and brain edema (Figures 6(h)–6(j))."
PCNA affects USP29
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1 |

No evidence text available
NeoR affects USP29
| 1
NeoR decreases the amount of USP29. 1 / 1
| 1

reach
"The purpose of positioning NeoR in this manner was to block the transcription of Usp29 with the Poly-A signal that had been included as part of NeoR."
N-nitrosomorpholine increases the amount of USP29. 1 / 1
1 |

No evidence text available
MKI67 affects HCT116
| 1
HCT116 binds USP29 and MKI67. 1 / 1
| 1

sparser
"We next checked the interactions between endogenous USP29 and Ki67 in HCT116 cells."
MIMT1 allele affects USP29
| 1
MIMT1 allele activates USP29. 1 / 1
| 1

reach
"Another suggested mechanism is the loss of imprinting (LOI) of PEG3 and/or USP29 mediated by silenced expression of the paternal MIMT1 allele."
MAVS affects USP29
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1 |

No evidence text available
MAP3K5 affects USP29
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1 |

No evidence text available
MACROH2A1 affects USP29
| 1
MACROH2A1 increases the amount of USP29. 1 / 1
| 1

reach
"It is plausible that macroH2A1 knockdown might have down-regulated the transcription of Peg3 and Usp29 through other unknown transcription factors like USF2."
KIF11 affects USP29
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1 |

No evidence text available
K48 affects ACSL5
| 1
USP29 binds ACSL5 and K48. 1 / 1
| 1

reach
"USP29 directly interacts with ACSL5 and stabilizes ACSL5 through the suppression of K48-linked ubiquitination.."
Il12p40 affects USP29
| 1
Il12p40 inhibits USP29. 1 / 1
| 1

reach
"Interestingly, the levels of cGAS and the expression of Tnf, Il6, Il12p40 and Ifit3 were significantly decreased in Trex1 -/- Usp29 m/m BMDCs compared to Trex1 -/- BMDCs (Supplementary information, Fig.S7d)."
IL6 affects USP29
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IL6 inhibits USP29. 1 / 1
| 1

reach
"Interestingly, the levels of cGAS and the expression of Tnf, Il6, Il12p40 and Ifit3 were significantly decreased in Trex1 -/- Usp29 m/m BMDCs compared to Trex1 -/- BMDCs (Supplementary information, Fig.S7d)."
IFIT3 affects USP29
| 1
IFIT3 inhibits USP29. 1 / 1
| 1

reach
"Interestingly, the levels of cGAS and the expression of Tnf, Il6, Il12p40 and Ifit3 were significantly decreased in Trex1 -/- Usp29 m/m BMDCs compared to Trex1 -/- BMDCs (Supplementary information, Fig.S7d)."
ICR affects USP29
| 1
ICR increases the amount of USP29. 1 / 1
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"Deletion of this ICR abolishes the transcription of Peg3 and Usp29, and also causes biallelic expression, or double dosage for some of the adjacent imprinted genes [18]."
Hypoxia affects USP29
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Hypoxia increases the amount of USP29. 1 / 1
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"19 In addition, the transcription of USP29 in gastric cancer cells could also be induced by TGF‐β, TNFα, and hypoxia, which were reported to be present in the tumor microenvironment and promote EMT and metastasis."
HSPA1B affects USP29
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No evidence text available
HRAS affects USP29
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No evidence text available
HR affects oncoprotein
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USP29 binds oncoprotein and HR. 1 / 1
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sparser
"The USP29, a DUB implicated in thiol-dependent hydrolysis, also interacts with HR HPV-16, -18 and -33 E7 oncoproteins [ xref ]."
HNF1B affects USP29
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HNF1B activates USP29. 1 / 1
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"However, USP29, a DUB that closely related to USP28, could not be induced by HNF-1beta."
HCT116 affects USP29
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HCT116 binds USP29 and MKI67. 1 / 1
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sparser
"We next checked the interactions between endogenous USP29 and Ki67 in HCT116 cells."
Glyphosate affects USP29
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Glyphosate increases the amount of USP29. 1 / 1
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No evidence text available
Gentamicins affects USP29
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Gentamicins decreases the amount of USP29. 1 / 1
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No evidence text available
FUBP1-USP29-AURKB affects USP29
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FUBP1-USP29-AURKB activates USP29. 1 / 1
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"We also investigated the molecular mechanism and identified a novel FUBP1-USP29-AURKB regulatory axis that enables the oncogenic role of USP29 in gastric cancer (Fig. 6)."
EPAS1 affects USP29
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No evidence text available
ECR18 affects USP29
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ECR18 activates USP29. 1 / 1
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"One ECR, ECR18, has been shown to physically interact with the promoter of Peg3/Usp29, and also recently demonstrated to be a shared enhancer between the paternally expressed Peg3/Usp29 and the maternally expressed Zim1 [17]."
E7 affects USP33
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USP29 binds USP33 and E7. 1 / 1
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sparser
"The Gaussia princeps luciferase protein complementation assay shows that USP15 interacts with the key oncoprotein human papillomavirus (HPV) E6, while USP29 and USP33 bind to E7 protein, suggesting that cellular DUBs impact HPV tumorigenesis by regulating the stability of the viral proteins [ xref ]."
CTSB affects USP29
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No evidence text available
CTNNB1 affects USP29
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No evidence text available
CTDSPL affects USP29
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No evidence text available
CTDSP2 affects USP29
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No evidence text available
CRISPR affects USP29
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CRISPR inhibits USP29. 1 / 1
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"The study has shown that CRISPR-mediated depletion of ubiquitin-specific peptidase 29 (USP29) leads to the disruption of intermediates accountable for involvement in glycolysis and nucleotide biosynthesis in neuroblastoma cell lines (Chandrasekaran et al., 2021)."
CEACAM6 affects USP29
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No evidence text available
CDK1 affects Thr578, and USP29
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CDK1 binds USP29 and Thr578. 1 / 1
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"First, we identify that CDK1 binds and phosphorylates USP29 at Ser575, Thr578, and Ser672, while the phosphorylation deficient 3A mutation almost abolishes the phosphorylation of USP29 (Figure 4 and Figure S4, Supporting Information)."
CCAR2 affects USP29
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No evidence text available

reach
"To investigate the effect of USP29 on hepatocyte apoptosis and inflammatory response in response to H/R stimulation in vitro, we overexpressed USP29 in primary hepatocytes by adenovirus infection and obtained USP29 knockout primary hepatocytes by isolating the livers of USP29-KO mice."
ACSL5 affects K48, and USP29
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USP29 binds ACSL5 and K48. 1 / 1
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"USP29 directly interacts with ACSL5 and stabilizes ACSL5 through the suppression of K48-linked ubiquitination.."
6-propyl-2-thiouracil decreases the amount of USP29. 1 / 1
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No evidence text available
2-palmitoylglycerol increases the amount of USP29. 1 / 1
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No evidence text available
4,4'-sulfonyldiphenol increases the amount of USP29. 1 / 1
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No evidence text available
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No evidence text available
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No evidence text available