IndraLab

Statements


USP19 affects FUNDC1
5 1 | 1 17 11
USP19 binds FUNDC1.
5 | 6 11
5 | 6 11

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"The interaction between ubiquitin-specific peptidase 19 (USP19) and FUNDC1 was analyzed using co-immunoprecipitation."

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"Plasmids HA-USP19 and Flag-FUNDC1 were co-transfected into BEAS-2B cells to elucidate the interaction between USP19 and FUNDC1."

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"A previous study demonstrated that USP19 interacts with FUNDC1 and stabilizes FUNDC1 [17]."

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"In the present study, FUNDC1 protein levels were decreased by USP19 knockdown, indicating that USP19 may be involved in mitophagy by regulating FUNDC1 expression in CSE-treated BEAS-2B cells.The interaction between USP19 and FUNDC1 was confirmed using the co-IP assays."

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sparser
"USP19 may interact with FUNDC1, which regulates mitophagy in COPD."

sparser
"A previous study demonstrated that USP19 interacts with FUNDC1 and stabilizes FUNDC1 [ xref ]."

sparser
"These results indicated that FUNDC1 interacts with USP19."

sparser
"Collectively, these results showed that USP19 interacts with FUNDC1 and stabilizes FUNDC1 in BEAS-2B cells."
USP19 deubiquitinates FUNDC1.
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USP19 deubiquitinates FUNDC1. 7 / 7
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"In response to hypoxia, <span class="match term0">USP19</span> binds to and deubiquitinates <span class="match term1">FUNDC1</span> at ER-mitochondria contact sites, which facilitates Drp1 oligomerization and Drp1 GTP-binding and hydrolysis activities, thereby promoting mitochondrial division"

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"In addition, in the initial phase of hypoxia, membrane associated ring–CH–type finger 5 (MARCH5) ubiquitinates FUNDC1 at Lys119, leading to its degradation [ 44 ], while USP19 deubiquitinates and stab[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"USP19 deubiquitinates FUNDC1."

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"To understand the deubiquitination of FUNDC1 by USP19, shUSP19 and/or Flag-FUNDC1 expression plasmids were co-transfected with His-Ub expression plasmids into BEAS-2B cells."

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"Under hypoxic conditions, USP19, an ER-resident, deubiquitinating enzyme, accumulates at the MERCs, binds to and deubiquitinates FUNDC1, which facilitates DRP1 oligomerization, GTP-binding, and GTP hydrolysis, to enhance mitochondrial division (Chai et al., 2021)."

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"In response to hypoxia, USP19 binds to and deubiquitinates FUNDC1 at ER-mitochondria contact sites, which facilitates Drp1 oligomerization and Drp1 GTP binding and hydrolysis activities, thereby promoting mitochondrial division."

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"In PINK1-Parkin-independent mitophagy: Upon conditions like hypoxia or loss of mitochondrial membrane potential, USP19 promotes the de-ubiquitination of FUNDC1, which promotes mitochondrial fission, leading to mitophagy 36."
USP19 deubiquitinates FUNDC1 on K119. 2 / 2
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"Meanwhile, during hypoxia, a small percentage of FUNDC1 protein is deubiquitinated by USP19 at Lys119, allowing FUNDC1 translocation to MAMs and DRP1-mediated mitochondrial division [115]."

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"USP19, a mitochondria-associated ER membrane (MAM) protein, accumulates at the ER-mitochondrial contact site, promotes localization of FUNDC1 to MAM, and deubiquitinates FUNDC1 at K119."
USP19 activates FUNDC1.
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USP19 activates FUNDC1. 2 / 2
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eidos
"In CSE-treated BEAS-2B cells , USP19 knockdown reduced FUNDC1 and LC3 II / I , increased the levels of TOM20 , improved cell survival , mitochondrial membrane potential , and OCR , and inhibited apoptosis ."

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"These findings indicate that USP19 promotes mitophagy by stabilizing FUNDC1 in CSE-treated BEAS-2B cells."
USP19 inhibits FUNDC1.
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USP19 inhibits FUNDC1. 1 / 1
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"Silencing USP19 alleviates cigarette smoke extract-induced mitochondrial dysfunction in BEAS-2B cells by targeting FUNDC1."
USP19 increases the amount of FUNDC1.
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USP19 increases the amount of FUNDC1. 1 / 1
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"In the present study, FUNDC1 protein levels were decreased by USP19 knockdown, indicating that USP19 may be involved in mitophagy by regulating FUNDC1 expression in CSE-treated BEAS-2B cells.The interaction between USP19 and FUNDC1 was confirmed using the co-IP assays."
USP19 affects BECN1
1 1 | 22 10
USP19 binds BECN1.
1 | 4 10
1 | 4 10

sparser
"Thus, an immunoprecipitation experiment was conducted to provide additional evidence for the potential interaction between USP19 and Beclin1."

sparser
"Resveratrol (Res), a polyphenol with multiple bioactivities, was demonstrated to diminish oxidative stress and Fe 2+ levels in I/R models and regulate USP19-Beclin 1 autophagy to inhibit ferroptosis [ xref ]."

sparser
"Beclin1 was detected among the proteins precipitated using USP19, suggesting an interaction between Beclin1 and USP19 (Fig. xref )."

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"The Beclin 1-ubiquitin-specific protease 19 (BECN1USP19) axis, a positive regulator of autophagy initiation and progression, plays a role in the crosstalk between autophagy and retinoic acid-inducible gene I (RIG-I) -mediated type I interferon signaling [ xref ]."

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"The IP results in this study showed that USP19 interacts with the Beclin1 protein to initiate autophagy in cells."

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"In addition, co-immunoprecipitation showed an interaction between USP19 and Beclin1."

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"Thus, an immunoprecipitation experiment was conducted to provide additional evidence for the potential interaction between USP19 and Beclin1."

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"Beclin1 was detected among the proteins precipitated using USP19, suggesting an interaction between Beclin1 and USP19 (Fig. 5B)."

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"Both phenomena indicated that the interaction between USP19 and Beclin1 induced by TIPE3 promoted cellular autophagy.M2-type macrophages, also known as anti-inflammatory and reparative macrophages, typically play a role in tissue repair, healing, and tumor microenvironment [29]."
USP19 deubiquitinates BECN1.
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USP19 deubiquitinates BECN1. 9 / 9
1 | 8

"<span class="match term0">USP19</span> modulates autophagy and antiviral immune responses by deubiquitinating <span class="match term1">Beclin-1</span>"

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"USP19 modulates autophagy and antiviral immune responses by deubiquitinating Beclin-1."

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"Ultimately, we identified that SIAH1-mediated degradation of USP19 reversed USP19-mediated deubiquitination of MAVS, Beclin1, TRAF3, and TRIF, resulting in the activation of antiviral immune responses."

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"USP19 can also deubiquitinate and stabilize Beclin 1 (BECN1), thereby promoting autophagy [65] ."

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"USP19, which catalyzes the hydrolysis of ubiquitin from its ubiquitin-conjugated protein substrates, is widely expressed in a variety of tissues, including liver, small intestine, and colon ( Combaret[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"USP19 modulates autophagy and antiviral immune responses by deubiquitinating Beclin-1."

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"Conversely, reversal of Beclin1 ubiquitylation by the DUB protein USP19 stabilized the protein under starvation conditions and promoted autophagy."

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"Deubiquitinase ubiquitin-specific protein 19 (USP19) is known to be involved in deubiquitinating Beclin1, TRAF3, and TRIF for downregulation of the type I IFN signaling."

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"However, the substrate(s) that mediate these effects of USP19 remain unknown.A recent report has shown that USP19 can deubiquitinate Beclin-1 and protect it from degradation in cultured non-muscle cel[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP19 activates BECN1.
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USP19 activates BECN1. 4 / 5
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"Beclin-1 stabilization is also promoted by USP19 and ataxin 3, which specifically removes K11- and K48-ubiquitin chain from Belcin-1, respectively [38, 39]."

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"Moreover, BECLIN-1 stability is increased by USP19, which counteracts Lys11-linked ubiquitination at lysine 437 [70] ."

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"USP19-mediated BECLIN-1 stabilization regulates not only the autophagy response to nutrient starvation but also the ability of BECLIN-1 to restrict innate immunity by inhibiting the RIG-I/MAVS/TBK1/IR[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"To check how C2-FFL, Beclin-1 early balance, and USP19 early balance modulated the positive rate of Beclin-1, we implemented the computational approach to abolish C2-FFL, downregulate Beclin-1 early b[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP19 ubiquitinates BECN1.
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USP19 ubiquitinates BECN1. 2 / 2
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"USP19 affects DDX58/RIG-I-mediated type I interferon signaling through ubiquitinating BECN1 and promoting the formation of autophagosomes [62]."

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"It has been found that USP19 promotes cervical cancer development by reducing the ubiquitination of Beclin-1."
USP19 increases the amount of BECN1.
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USP19 increases the amount of BECN1. 2 / 2
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"Western blotting experiments showed that the elevated Beclin1 expression level after TIPE3 overexpression in LoVo and SW480 cells was decreased by USP19 silencing (Fig. 5E)."

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"Moreover, Beclin1 protein expression, which was elevated by TIPE3 overexpression, was decreased by USP19 silencing."
USP19 inhibits BECN1.
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USP19 inhibits BECN1. 1 / 1
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"In another study, USP19 was reported to rescue the proteasomal degradation of BECN1 through removing the K11-linked ubiquitination [49]."
USP19 decreases the amount of BECN1.
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USP19 decreases the amount of BECN1. 1 / 1
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"Western blotting and immunofluorescence experiments of proteins related to autophagy showed that USP19 overexpression substantially reduced the protein expression of P62 in LoVo and SW480 cells while significantly elevating the protein expression of Beclin1 and LC3, as compared to the L-OHP group (Fig. 4B–D)."
USP19 affects BAG6
6 | 16 4
USP19 binds BAG6.
6 | 3 3
6 | 3 3

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"We also have conducted an IP/WB assay to showcase the interaction between USP19 and BAG6 in both breast normal and TNBC cell lines (MCF‐10A and MDA‐MB‐231) (Figure 3I,J)."

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"15 Here, we showed that USP19 binds to BAG6 and decreases BAG6 ubiquitination, which prevents BAG6 from being degraded in TNBC tumour tissue and BC cells."

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sparser
"Next, we examined the binding association of BAG6 and USP19 in cells by IP‐Western blot analysis."

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sparser
"We also have conducted an IP/WB assay to showcase the interaction between USP19 and BAG6 in both breast normal and TNBC cell lines (MCF‐10A and MDA‐MB‐231) (Figure  xref )."

sparser
"We also explored whether BAG6 interacts with USP19 mRNA directly."

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USP19 deubiquitinates BAG6.
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USP19 deubiquitinates BAG6. 7 / 7
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"Deubiquitinase USP19 modulates apoptotic calcium release and endoplasmic reticulum stress by deubiquitinating BAG6 in triple negative breast cancer."

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"Overexpression of USP19 significantly decreased ubiquitination of BAG6, but increased BAG6 protein level when compared with the control group (Figure 4A)."

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"Next, to investigate whether the ability of USP19 to deubiquitinate BAG6 was dependent on its protease activity, we transfected WT‐USP19 or its mutants into cells."

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"Overexpression of WT‐USP19 reduced BAG6 ubiquitination, but transfection with the C607S‐USP19 plasmid had no effect on BAG6 ubiquitination (Figure 4B)."

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"28 , 29 Overexpression of USP19 significantly inhibited the WT and K29‐linked polyubiquitination of BAG6, but did not affect the other ubiquitination linkages (K6, K11, K27, K33, K48 or K63) in BAG6 (Figure 4C)."

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"This was in agreement with USP19 knockdown increasing K29‐linked ubiquitination of BAG6 (Figure 4D)."

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"In this study, we found, for the first time, that USP19 reduced ubiquitination and degradation of BAG6 to induce BC cell apoptosis and ER stress."
USP19 decreases the amount of BAG6.
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USP19 decreases the amount of BAG6. 4 / 4
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"The reduction in BAG6 protein levels caused by USP19 knockdown was reversed after treatment with proteasome inhibitor MG132."

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"Knockdown of USP19 gene did not cause changes in BAG6 protein levels with lysosome inhibitor Chloroquine (CQ) (Figure S3B)."

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"Silencing and overexpressing USP19 significantly reduced BAG6 protein levels, despite having no effect on BAG6 mRNA levels (Figure 3F and Figure S3A), suggesting that USP19 regulates BAG6 not at the transcript but at the protein translation level."

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"Overexpression of USP19 significantly decreased ubiquitination of BAG6, but increased BAG6 protein level when compared with the control group (Figure 4A)."
USP19 increases the amount of BAG6.
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USP19 increases the amount of BAG6. 2 / 2
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"Therefore, we next investigated the molecular mechanism by which USP19 induces changes in the ER stress by affecting the expression of BAG6."

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"Overexpression of USP19 significantly decreased ubiquitination of BAG6, but increased BAG6 protein level when compared with the control group (Figure 4A)."
USP19 ubiquitinates BAG6.
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USP19 ubiquitinates BAG6. 1 / 1
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sparser
"To further clarify the regulatory role of BAG6 ubiquitination by USP19, MDA‐MB‐231 cells were co‐transfected with Myc‐ USP19 (wild‐type, WT) or Myc‐C607S mutant, xref Flag‐ BAG6 , and HA‐Ub."
FUNDC1 affects USP19
5 | 7 11
FUNDC1 binds USP19.
5 | 6 11
5 | 6 11

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"The interaction between ubiquitin-specific peptidase 19 (USP19) and FUNDC1 was analyzed using co-immunoprecipitation."

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"Plasmids HA-USP19 and Flag-FUNDC1 were co-transfected into BEAS-2B cells to elucidate the interaction between USP19 and FUNDC1."

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"A previous study demonstrated that USP19 interacts with FUNDC1 and stabilizes FUNDC1 [17]."

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"In the present study, FUNDC1 protein levels were decreased by USP19 knockdown, indicating that USP19 may be involved in mitophagy by regulating FUNDC1 expression in CSE-treated BEAS-2B cells.The interaction between USP19 and FUNDC1 was confirmed using the co-IP assays."

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sparser
"USP19 may interact with FUNDC1, which regulates mitophagy in COPD."

sparser
"A previous study demonstrated that USP19 interacts with FUNDC1 and stabilizes FUNDC1 [ xref ]."

sparser
"These results indicated that FUNDC1 interacts with USP19."

sparser
"Collectively, these results showed that USP19 interacts with FUNDC1 and stabilizes FUNDC1 in BEAS-2B cells."
FUNDC1 activates USP19.
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FUNDC1 activates USP19. 1 / 1
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"FUNDC1 overexpression inhibited the effect of USP19 knockdown in CSE-treated BEAS-2B cells."
USP19 affects MGMT
1 | 12 9
USP19 binds MGMT.
1 | 3 8
1 | 3 8

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"Furthermore, our results show that USP19MGMT axis related to the MGMT‐positive GBM cell response to TMZ treatment."

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"To gain more proof to certify the biological function of USP19MGMT axis in GBM cells chemo‐resistance in vivo, we employed shRNA to specifically knockdown USP19 in T98G cells, and nude mice were subcutaneously implanted with T98G USP19 depletion cells and control (Ctrl) cells."

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"Mechanistically, USP19 binds to MGMT and then deubiquitinates MGMT, causing the removal of ubiquitin chains, which stabilize MGMT."

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"Additionally, endogenous immunoprecipitation further confirmed that MGMT binds to USP19 (Figure 2B)."

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"To further investigate the mechanism of binding between USP19 and MGMT, we found that the N terminal (1‐596aa) and UI domain of USP19 were responsible for binding with MGMT (Figure 2C).2.3 USP19 deubiquitinates MGMT in vivo and in vitro."

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"Since USP19MGMT axis regulates the sensitivity of GBM to TMZ, we analyzed the overall survival of GBM patients in different USP19 expression backgrounds."

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"Mechanistically, USP19 binds to MGMT and then deubiquitinates MGMT, causing the removal of ubiquitin chains, which stabilize MGMT."

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"In this study, we found that the DNA O6‐methylation lesions repair is dependent on the USP19MGMT axis, which is a new perspective to intervene in the mechanism of alkylation lesions repair, especially the TMZ treatment for GBM patients."

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"Our study reports the potential of USP19MGMT axis as a new chemotherapy target and biomarker in GBM patients."

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USP19 ubiquitinates MGMT.
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USP19 ubiquitinates MGMT. 3 / 3
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"Conversely, overexpressing USP19 WT in cell resulted in a significant decrease in polyubiquitination of MGMT, while overexpressing USP19 CS mutant failed to alter the level of MGMT polyubiquitination (Figure 3B)."

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"The regulation of MGMT ubiquitination by USP19 plays a critical role in DNA methylation damage repair and GBM patients' temozolomide chemotherapy response."

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"The regulation of MGMT ubiquitination by USP19 plays a critical role in DNA methylation damage repair and GBM patients’ temozolomide chemotherapy response."
USP19 deubiquitinates MGMT.
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USP19 deubiquitinates MGMT. 3 / 3
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"Mechanistically, USP19 binds to MGMT and then deubiquitinates MGMT, causing the removal of ubiquitin chains, which stabilize MGMT."

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"To further investigate the mechanism of binding between USP19 and MGMT, we found that the N terminal (1‐596aa) and UI domain of USP19 were responsible for binding with MGMT (Figure 2C).2.3 USP19 deubiquitinates MGMT in vivo and in vitro."

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"To further clarify the effect of USP19‐mediated MGMT stabilization, we assessed whether USP19 deubiquitinated MGMT in cells."
USP19 increases the amount of MGMT.
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USP19 increases the amount of MGMT. 2 / 2
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"We found that knockdown of USP19 dramatically decreased MGMT protein level, which can be rescued by proteasome inhibitor MG132 (Figure 1B)."

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"As shown in Figure 1C,D, depletion of endogenous USP19 by two USP19‐specific shRNAs markedly decreased protein level, but not the MGMT mRNA level, suggesting that USP19 regulates MGMT through post‐translational modification."
USP19 activates MGMT.
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USP19 activates MGMT. 2 / 2
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"As shown in Figure 1E,F, MGMT stability was dramatically decreased in USP19‐depleted cells, while reconstitution of USP19 could rescue MGMT protein stability in USP19‐depleted cells."

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"To further clarify the effect of USP19‐mediated MGMT stabilization, we assessed whether USP19 deubiquitinated MGMT in cells."
USP19 affects ME1
3 | 12 7
USP19 binds ME1.
3 | 2 7
3 | 2 7

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"Next, we studied whether ERK2 affected the interaction between ME1 and USP19."

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"IP results showed that EGF treatment dramatically enhanced USP19-ME1 interaction in NCM460 cells ( Figure S2 R)."

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"The endogenous interactions between USP19 and ME1 were confirmed by co-IP experiments in CRC HCT116 and LoVo cells ( Figures 1 B and S1 B)."

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"Next, we studied whether ERK2 affected the interaction between ME1 and USP19."

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"These results are consistent with the proteomic and biochemical findings, discussed above, and support the idea that the USP19-ME1 axis mediates lipogenesis and plays a critical role in both murine an[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Finally, the authors showed that the USP19-ME1 signaling axis is dysregulated in human colorectal cancer samples, and that USP19 is upregulated during colorectal carcinogenesis pathogenesis and spontaneous tumor development."

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"The endogenous interactions between USP19 and ME1 were confirmed by co-IP experiments in CRC HCT116 and LoVo cells ( Figures 1 B and S1 B)."
USP19 activates ME1.
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USP19 activates ME1. 5 / 5
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"USP19-mediated ME1 stabilization is enhanced by phosphorylation, generating oncogenic phenotypes, and either USP19 deletion or a point mutation in ME1 protein that prevents ubiquitination, represses colorectal carcinogenesis."

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"Meanwhile, depletion of USP19 significantly reduced ME1 protein half-life in CRC cells ( Figures 1 D, 1E, and S1 F)."

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"These findings suggest that USP19 promotes the stability of ME1 through direct interaction and deubiquitination.The foregoing results indicate that USP19 deubiquinates ME1, but whether E3 ubiquitin li[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Ubiquitin-specific peptidase 19 (USP19) antagonizes ring finger protein 1 (RNF1)-mediated ME1 degradation by deubiquitination, thereby promoting lipid metabolism and NADPH production, and suppressing ROS, thereby promoting colorectal carcinogenesis [141]."

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"In addition, USP19 antagonizes ring finger protein 1 (RPF1)-mediated malic enzyme 1 (ME1) degradation through deubiquitination, which in turn promotes lipid metabolism associated with ERK2 activity and CRC development in human patients [139]."
USP19 deubiquitinates ME1.
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USP19 deubiquitinates ME1. 3 / 3
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"Ectopic USP19 expression did not repress ME1 ubiquitination following ERK2 depletion ( Figure S2 O)."

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"Further studies indicated that the K60 site of ME1 was deubiquitinated by USP19 ( Figure 1 G)."

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"Our data show that USP19 deubiquitinates and stabilizes ME1 to promote lipogenesis and colorectal carcinogenesis.Previous work has shown ERK2 is a central and terminal master kinase that mediates mult[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP19 increases the amount of ME1.
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USP19 increases the amount of ME1. 2 / 2
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"We found that USP19 inhibition significantly decreased ME1 protein levels in CRC cells ( Figures 1 C and S1 E) and that they could be rescued by wild-type (WT) USP19 but not by a ubiquitination-defect[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"On the other hand, USP19 inhibition severely retarded CRC cell growth in MTT assay and reduced the expression of ME1, while these defects were successfully rescued after ME1 overexpression ( Figures S[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP19 is modified
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USP19 is palmitoylated.
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USP19 is palmitoylated. 10 / 14
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"Then, we found that ZDHHC2 decreases the lipid peroxide production and suppresses ferroptosis through mediating the S‐palmitoylation of USP19 and destabilizing ACSL4 in CRPC cells."

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"To confirm whether ZDHHC2 is the only ZDHHC enzyme that mediates the palmitoylation of USP19, a set of ZDHHC‐knockout cell lines was established using a CRISPR screen."

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"We discovered that USP19 inhibits the ubiquitin–proteasome pathway‐mediated degradation of ACSL4 and that ZDHHC2 enhances ACSL4 degradation by palmitoylating USP19, ultimately suppressing ferroptosis."

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"ZDHHC2 Mediates the Palmitoylation of USP19."

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"Therefore, we propose that USP19 palmitoylation attenuates its interaction with ACSL4, thereby facilitating ACSL4 ubiquitination and subsequent degradation."

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"Under enzalutamide resistance, the FOXA1/CXXC5/TET2 complex induces aberrant upregulation of ZDHHC2, which subsequently suppresses ferroptosis by mediating S‐palmitoylation of USP19 and degradation of ACSL4 protein in CRPC cells."

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"We found that palmitic acid elevated palmitoylation of USP19 (Figure  xref , Supporting Information) and reduced ACSL4 protein level (Figure  xref , Supporting Information), and these effects disappeared after ZDHHC2 knockout (Figure  xref , Supporting Information)."

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"ZDHHC2 is transcriptionally upregulated by a FOXA1/CXXC5/TET2 complex and promotes S‐palmitoylation of the deubiquitinase USP19, which impairs its interaction with ACSL4."

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"In the resistant cell lines, mRNA level of USP19 and ACSL4 remained unchanged (Figure  xref , Supporting Information), palmitoylation of USP19 was elevated, and ACSL4 protein was decreased (Figure  xref , Supporting Information)."

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"Only ZDHHC2 knockout significantly abolished the palmitoylation of USP19 (Figure  xref ; Figure  xref , Supporting Information)."
USP19 is ubiquitinated.
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USP19 is ubiquitinated. 3 / 3
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"Recent studies have demonstrated that SIAH1 undergoes K29-linked auto-ubiquitination and facilitates K27-linked ubiquitination of USP19 for proteasomal degradation ( xref )."

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"Previous study has been reported that SIAH2 is a binding partner of USP19 and promotes USP19 ubiquitylation and proteasome‐dependent degradation xref ; thus, we hypothesize USP19 may be regulated by SIAH2 in response to cardiac hypertrophy stimulation."

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"In the early stages of virus infection, stabilized SIAH1 directly interacts with the USP19 and simultaneously mediates K27-linked ubiquitination of 489, 490, and 610 residues of USP19 for proteasomal degradation."
USP19 is phosphorylated.
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USP19 is phosphorylated on S244. 2 / 2
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USP19 is phosphorylated on T407. 1 / 1
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USP19 is methylated.
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USP19 is methylated. 2 / 2
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"METTL14 reduced m 6 A RNA methylation of USP19 ."

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"At a raw p -value < 0.05 for the present study, only a few associations replicated in the same direction as previous PFAS epigenome-wide association studies: PFOS with increased methylation in ANO3 (cg05146852) [ xref ] and PFNA with decreased methylation in HIF1A (cg04509825) and TTL (cg03065329) and increased methylation in PTGIS (cg27059136) and USP19 (cg01673931) [ xref ]."

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"These results indicated that USP19 enhanced cell proliferation and exhibited anti-apoptotic properties."

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"In addition, depletion of USP19 reduces cell proliferation and causes cell arrest at the G G1 phase and p27 stabilization [112]."

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"Reports indicate that the disruption of USP19 inhibits a series of PCa cell proliferation by arresting cells in the G1 to S phase transition through stabilization of the cyclin-dependent kinase inhibitor p27 ."

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"USP19 deubiquitinating enzyme supports cell proliferation by stabilizing KPC1, a ubiquitin ligase for p27Kip1."

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"We previously reported that the USP19 deubiquitinating enzyme positively regulates proliferation in fibroblasts by stabilizing KPC1, a ubiquitin ligase for p27 Kip1."

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"In addition, CCK8 assay also proved that USP19 depletion inhibited the proliferation rate of Hep3B and Huh7 cells ( Fig. 5 E)."

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"The results showed that knockdown of USP19 suppressed cell proliferation in SGC7901 cells while overexpressing USP19 accelerated cell growth in GES1 cells, as determined by MTT assay (P < 0.01, XREF_FIG and XREF_FIG)."

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"Figure 5C, D shows that the cell proliferation and viability enhanced by TIPE3 overexpression was inhibited by silencing USP19."

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"Correspondingly, USP19 knockdown decreased proliferation, migration of HCC cells, which could be reversed by overexpression of YAP."

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"Flow cytometry was used to investigate the role of cell‐cycle disruption in the increased proliferation caused by USP19 overexpression."

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"Depletion of USP19 inhibited proliferation in prostate cancer DU145, PC-3 and 22RV1 cells, which was similar to the pattern established in fibroblasts in that it was due to decreased progression from G1 to S phase and associated with a stabilization of the cyclin-dependent kinase inhibitor p27 Kip1 ."

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"Depletion of USP19 inhibited proliferation in prostate cancer DU145, PC-3 and 22RV1 cells, which was similar to the pattern established in fibroblasts in that it was due to decreased progression from G1 to S phase and associated with a stabilization of the cyclin dependent kinase inhibitor p27 Kip1."

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"USP19 was shown to inhibit cell proliferation, while inducing apoptosis by reducing ubiquitination and degradation of BAG6 and enhancing BCL2 induced ER stress by elevating the intracellular Ca concentration."

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"USP19 deubiquitinates and thereby stabilizes the KPC1 ligase for p27 Kip1, and so indirectly promotes degradation of p27 Kip1 and subsequent cell proliferation."

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"We also found that USP19 overexpression inhibits cell proliferation, induces apoptosis, and regulates cell‐cycle assay in TNBC cells."

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"USP19 was significantly regulated in MDA‐MB‐231 compared with the levels detected in the control groups (Figure S1F).3.2 USP19 suppressed cell proliferation and induced apoptosis of BC cell lines."

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"Opposite to our findings, Hu and collaborators recently demonstrated that USP19 negatively regulates proliferation and migration in clear cell renal carcinoma 69."

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"We demonstrated that expression of USP19 inhibited proliferation and induced apoptosis in TNBC cells, as well as promoting BAG6 protein expression by deubiquitinating modification."
USP19 affects PAH
6 | 13 1
USP19 binds PAH.
6 | 2 1
6 | 2 1

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"A Duolink in situ proximity ligation assay (PLA) kit (DUO92101, Sigma-Aldrich) was used to detect the interaction between PAH and USP19 and to evaluate the ubiquitination status of PAH."

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"Our study indicates that USP19 interacts with PAH and prevents polyubiquitination of PAH subsequently extending the half-life of PAH protein."

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"As shown in Fig. 3b, in situ USP19 and PAH interaction was observed in the form of PLA dots when USP19 and PAHwt or the PAH variants were immunostained together (Fig. 3b, left panel), but not when they were stained with either USP19 or PAH antibody alone (Fig. 3b, right panel)."

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USP19 increases the amount of PAH.
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USP19 increases the amount of PAH. 4 / 4
| 4

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"Overall, our results suggest that USP19 increases the cellular protein level of PAH variants by extending their half-lives, which results in increased Phe metabolism."

reach
"We found that ectopic expression of USP19 increased PAH protein level, whereas depletion of USP19 promoted PAH protein degradation."

reach
"Therefore, we initiated this study to investigate whether USP19, a previously identified DUB for PAHwt , can increase cellular levels of PAH variants through deubiquitinating activity.Notably, USP19 is an endoplasmic reticulum (ER)-specific DUB and participates in the export of misfolding-associated protein, which are associated with neurodegenerative diseases ."

reach
"Therefore, we investigated the functional consequence of USP19-mediated increase in the protein levels of the PAH variants on their metabolic function."
USP19 activates PAH.
| 4
USP19 activates PAH. 2 / 2
| 2

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"The deubiquitinating activity of USP19 prevents protein degradation and increases the abundance of both PAH protein variants."

reach
"USP19 increases the enzymatic activity of PAH, thus maintaining normal Phe levels."
USP19 activates PAH-R243Q. 1 / 1
| 1

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"We also demonstrate that USP19 deubiquitinates and rescues both the R241C and R243Q PAH variants from rapid protein degradation, whereas inhibiting USP19, either by sgRNA-mediated knockdown or by broad spectrum DUB inhibitor, promoted the ubiquitination of these two PAH variants."
USP19 activates PAH-R241C. 1 / 1
| 1

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"We also demonstrate that USP19 deubiquitinates and rescues both the R241C and R243Q PAH variants from rapid protein degradation, whereas inhibiting USP19, either by sgRNA-mediated knockdown or by broad spectrum DUB inhibitor, promoted the ubiquitination of these two PAH variants."
USP19 deubiquitinates PAH.
| 3
USP19 deubiquitinates PAH. 3 / 3
| 3

reach
"Thus our results indicate that USP19 deubiquitinates PAHwt and PAH variants and rescues them from rapid proteasomal degradation."

reach
"Our study indicates that USP19 interacts with PAH and prevents polyubiquitination of PAH subsequently extending the half-life of PAH protein."

reach
"USP19 deubiquitinates and stabilizes PAH further protecting it from rapid degradation."
USP19 affects BIRC5
3 | 1 8 8
USP19 binds BIRC5.
3 | 7 8
3 | 6 8

sparser
"To assess the mechanism of this possible interaction between USP19 and survivin, we next treated HCC827 cells with MK2206, an AKT activity inhibitor, finding that the binding of USP19 to survivin was [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Survivin T34A mutant impaired the binding of USP19 to survivin in the presence of gastrodin ( Fig. 4 G)."

sparser
"These data suggest that gastrodin induces NSCLC apoptosis by interfering with the binding of USP19 to Survivin Thr34."

No evidence text available

No evidence text available

sparser
"Our present study found that gastrodin treatment interfered with USP19 binding to survivin, promoting survivin ubiquitination and apoptosis."

sparser
"Moreover, gastrodin can inhibit the interaction between USP19 and survivin to stimulate the destruction and ubiquitination of survivin."

reach
"This observation supported the notion that gastrodin-induced apoptosis in NSCLC cells by interfering with the binding of USP19 to survivin."

reach
"To assess the mechanism of this possible interaction between USP19 and survivin, we next treated HCC827 cells with MK2206, an AKT activity inhibitor, finding that the binding of USP19 to survivin was [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Survivin T34A mutant impaired the binding of USP19 to survivin in the presence of gastrodin ( Fig. 4 G)."
USP19 binds BIRC5 and A549. 1 / 1
| 1

reach
"Therefore, we first verified the potential effects of gastrodin on the interaction between USP19 and survivin in A549 cells."
USP19 inhibits BIRC5.
| 1
USP19 inhibits BIRC5. 1 / 1
| 1

eidos
"Survivin Thr34 dephosphorylation caused by gastrodin interfered with the binding of ubiquitin-specific protease 19 ( USP19 ) , which eventually destabilized survivin ."
USP19 deubiquitinates BIRC5.
| 1
USP19 deubiquitinates BIRC5. 1 / 1
| 1

reach
"USP19 deubiquitinates survivin protein and extends its half-life."
USP19 affects CORO2A
1 | 10 6
USP19 binds CORO2A.
| 5 6
| 5 4

reach
"These results show that the binding of CORO2A and USP19 was not dependent on both normal and cancer cells."

reach
"Together, our results demonstrate the importance of the interaction between USP19 and CORO2A in the regulation of RAR, which is possibly involved in adipogenesis."

sparser
"These results show that the binding of CORO2A and USP19 was not dependent on both normal and cancer cells."

sparser
"USP19 is associated with the ubiquitination of CORO2A."

sparser
"Co-immunoprecipitation assay revealed that USP19 strongly binds with CORO2A (Figure xref and xref )."

sparser
"Together, our results demonstrate the importance of the interaction between USP19 and CORO2A in the regulation of RAR, which is possibly involved in adipogenesis."

reach
"We next validated the association between USP19 and CORO2A, and the regulation of CORO2A by USP19."

reach
"Co-immunoprecipitation assay revealed that USP19 strongly binds with CORO2A."

reach
"We next evaluated the endogenous binding between CORO2A and USP19."
USP19 binds CORO2A and RAR. 2 / 2
| 2

sparser
"The results suggest that USP19 may be associated with the transcriptional regulation of RAR via CORO2A as one of the components for the NCoR complex during the adipogenesis."

sparser
"These results indicate that USP19 is associated with the regulation of transcriptional repression of RAR through CORO2A."
USP19 activates CORO2A.
| 3
USP19 activates CORO2A. 3 / 5
| 3

reach
"Overexpression of USP19 inhibited RARE transcription and knock-down of USP19 using siRNA showed dramatically increased transcription of RARE, suggesting that USP19 increases the stability of CORO2A by DUB activity, and that binding of these two protein may be associated with the function of NCoR."

reach
"These results indicate that USP19 may prevent proteasomal degradation of CORO2A."

reach
"The result showed that CORO2A was gradually increased by USP19 expression."
USP19 deubiquitinates CORO2A.
1 | 1
USP19 deubiquitinates CORO2A. 2 / 2
1 | 1

"<span class="match term0">UBIQUITIN SPECIFIC PROTEASE 19</span> involved in transcriptional repression of retinoic acid receptor by stabilizing <span class="match term1">CORO2A</span>"

reach
"And USP19 deubiquitinated CORO2A, suggesting that USP19 might have function to stabilize NCoR co and repressor complex in cells."
USP19 decreases the amount of CORO2A.
| 1
USP19-C506S decreases the amount of CORO2A. 1 / 1
| 1

reach
"However, the catalytic mutant USP19 (C506S) did not significantly decrease the ubiquitination level of CORO2A."
| 17 2
Genome polyprotein increases the amount of USP19.
| 12 2
Genome polyprotein increases the amount of USP19. 10 / 14
| 12 2

sparser
"Daidzein activated ERβ-dependent transcriptional activity in vitro, and inhibited E2-induced USP19 mRNA expression in vitro and in vivo ."

reach
"E2 induced ubiquitin specific peptidase 19 (USP19) expression during myogenesis."

reach
"Furthermore, in skeletal muscle satellite cells, E2 inhibited myogenesis and increased USP19 expression, and diarylpropionitrile repressed E2 increased USP19 expression."

reach
"Administration of E2 to OVX mice decreased muscle mass ratio and increased USP19 mRNA expression in the soleus muscle of OVX mice."

reach
"Ogawa et al. report that E2 treatment of ovx mice decreased the ratio of skeletal muscle mass to body weight and increased ubiquitin-specific peptidase 19 expression, indicating that E2 inhibits myogenesis in vivo."

reach
"Also, an E2 dose-dependent increase in USP19 mRNA expression was observed in the myoblast cell line C2C12 [ 47 ]."

reach
"These results demonstrate that in soleus muscle in young female mice under physiological conditions, E2 upregulates USP19 expression through ERalpha and consequently leads to decreases in ubiquitin conjugates and muscle mass."

reach
"E2 replacement increased USP19 expression in the gastrocnemius and soleus muscles of ovariectomized mice."

reach
"Likely, in OVX mice, E2 up-regulated USP19 protein expression, and daidzein attenuated E2-dependent increase of USP19 protein expression ( Fig. 5 D)."

reach
"These results indicate that in soleus muscle of OVX mice, daidzein inhibits the E2-dependent increase in USP19 expression through ERβ and the E2-depdentnt decrease in muscle ratio.USP19 expression is [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
| 5

reach
"C2C12 myoblasts were cultured in steroid-free differentiation medium in 48-well plates, and transiently transfected with pUSP19-hERE and pGL4.73[hRluc/SV40] with HilyMax for 24 h. To examine the effec[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Curiously, E2 upregulated the deubiquitinating enzyme ubiquitin-specific peptidase 19 (USP19) mRNA expression in the soleus and gastrocnemius muscles of female ovariectomized mice and decreased the ra[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"E2 and PPT, but not DPN, increased luciferase reporter activity ( Fig. 3 A ), consistent with the finding that E2 increases Usp19 reporter activity through ERα [17] ."

reach
"Taken together, ERβ agonists seem to have a generally beneficial effect on muscle mass, but might exert distinct effects on E2-induced USP19 expression.Two ER isoforms were expressed in the soleus mus[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Taken together, ERβ agonists seem to have a generally beneficial effect on muscle mass, but might exert distinct effects on E2-induced USP19 expression.Gain- and loss-of-function approaches have demon[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP19 affects TARDBP
| 17 1
USP19 increases the amount of TARDBP.
| 11
USP19 increases the amount of TARDBP. 10 / 11
| 11

reach
"Interestingly, Xu et al., (2018) also observed that co-expression of USP19 with wild type TDP-43, barely increased the amount of TDP-43 in the extracellular medium [28]."

reach
"Conversely, when co-expressed with the pathological TDP-43-K263E (Fig. 1b), USP19-WT, but not the USP19-ΔTM, significantly increased the secretion of aggregated TDP-43 (Fig. 1d, e)."

reach
"Interestingly, when the same experiments were conducted with the C-terminus prion-like domain (GFP-TDP-43-CTF ) prone to high aggregation [35] (see Fig. S3a), neither USP19-WT nor its mutants stimulated TDP-43 secretion (Fig. S3d)."

reach
"The data presented above revealed that USP19-WT promotes the secretion of misfolded TDP-43 but the mechanisms by which USP19-WT induces this secretion are still unresolved."

reach
"The vesicular SNARE VAMP7 regulates the secretion of misfolded TDP-43 mediated by USP19."

reach
"The DNAJC5/CSPalpha chaperone protein modulates the release of TDP-43 aggregates mediated by USP19."

reach
"USP19 overexpression promotes the secretion of soluble and free TDP-43 aggregates."

reach
"This indicates that USP19 does not promote the release of misfolded TDP-43 through the exosomal pathway."

reach
"The fact that TDP-43 was found to be released in a VAMP7-dependent manner is certainly coherent with this notion.In our study, we observed that inhibiting DNAJC5/CSPα function using a dominant negative construct (S10A-CSPα), or through DNAJC5/CSPα siRNAs, significantly reduced the release of misfolded TDP-43 mediated by USP19."

reach
"Nevertheless, the implication of early autophagy processes was further supported by a significant decrease in USP19-mediated misfolded TDP-43 secretion upon inhibition of ATG7, RAB11A or VAMP7 components, all of which are involved in the early steps of phagophore/autophagosome biogenesis [47, 59, 70]."
USP19 activates TARDBP.
| 4
USP19 activates TARDBP. 4 / 4
| 4

reach
"These findings strongly suggest that the USP19-mediated MAPS pathway conducts a specific sorting of misfolded TDP-43, leading to its secretion as soluble and free aggregates rather than through exosomes.The exact molecular and cellular mechanisms by which cells select misfolded proteins for secretion via the USP19 dependent MAPS pathway remain enigmatic."

reach
"Our investigations revealed that USP19-WT promotes the secretion of soluble and aggregated forms of misfolded TDP-43 without associated cell mortality."

reach
"Consistently, we found that USP19-WT promotes the secretion of free aggregates as well as soluble misfolded TDP-43 in the extracellular medium."

reach
"As anticipated, little or no TDP-43 signal was observed when USP19-ΔTM was expressed.In conclusion, these findings indicate that USP19-WT expression promotes the secretion of both soluble and aggregated forms of misfolded TDP-43."
USP19 binds TARDBP.
| 1 1
| 1 1

sparser
"TDP-43 is associated with USP19 and the ER compartments and is engulfed in different intracellular compartments."

reach
"Additionally, co-immunoprecipitation experiments demonstrated interactions between USP19 and TDP-43 thus suggesting that part of TDP-43 could be secreted in a form of a tri-complex ER-USP19-TDP-43 engulfed into secretory compartments.To gain a clearer understanding of the cellular mechanisms involved in the misfolded TDP-43 secretion via the MAPS pathway, we evaluated the impact of the Spautin1 and LY294002 inhibitors on the early steps of phagophores/autophagosomes biogenesis [45, 46]."
USP19 decreases the amount of TARDBP.
| 1
USP19 decreases the amount of TARDBP. 1 / 1
| 1

reach
"Our results showed a strong inhibition of USP19-mediated misfolded TDP-43 secretion, indicating a crucial role of early autophagosomal compartments."
USP19 affects SYVN1
2 1 | 8 3
USP19 binds SYVN1.
2 | 2 3
2 | 2 3

sparser
"However, there was no association between Myc-USP19 and FLAG-Nixin detected ( xref B), supporting the specificity of the USP19 interaction with HRD1."

sparser
"These results suggest that USP19 interacts with HRD1."

reach
"These results suggest that USP19 interacts with HRD1."

reach
"We reported that USP19 interacts with and targets HRD1 for the deubiquitination of K48 linked ubiquitin chains, the primary signal for proteasomal degradation [XREF_BIBR, XREF_BIBR] (XREF_FIG A and XREF_FIG A)."

No evidence text available

sparser
"USP19 Interacts with HRD1."

No evidence text available
USP19 deubiquitinates SYVN1.
1 | 4
USP19 deubiquitinates SYVN1. 5 / 5
1 | 4

reach
"USP19 negatively regulates the ubiquitination of HRD1 and prevents it from undergoing proteasomal degradation."

"We demonstrate that <span class="match term0">USP19</span> rescues <span class="match term1">HRD1</span> from proteasomal degradation by deubiquitination of K48-linked ubiquitin chains"

reach
"These results suggest that USP19 deubiquitinates HRD1."

reach
"USP19 Deubiquitinates HRD1."

reach
"USP19 deubiquitinates HRD1, rescuing it from UPS-mediated degradation [153], while USP13 works in concert with the ERAD E3 ligase GP78 to maintain precise ubiquitination of the cytosolic ERAD component UBL4A [154]."
USP19 activates SYVN1.
| 2
USP19 activates SYVN1. 2 / 4
| 2

reach
"In this study, we found that overexpression of USP19 also increases the stability and steady-state expression levels of HRD1 in a manner dependent on its DUB activity (XREF_FIG A, XREF_FIG A and Figure S7)."

reach
"We demonstrate that USP19 rescues HRD1 from proteasomal degradation by deubiquitination of K48 linked ubiquitin chains."
BIRC5 affects USP19
3 | 7 8
3 | 6 8

sparser
"To assess the mechanism of this possible interaction between USP19 and survivin, we next treated HCC827 cells with MK2206, an AKT activity inhibitor, finding that the binding of USP19 to survivin was [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Survivin T34A mutant impaired the binding of USP19 to survivin in the presence of gastrodin ( Fig. 4 G)."

sparser
"These data suggest that gastrodin induces NSCLC apoptosis by interfering with the binding of USP19 to Survivin Thr34."

No evidence text available

No evidence text available

sparser
"Our present study found that gastrodin treatment interfered with USP19 binding to survivin, promoting survivin ubiquitination and apoptosis."

sparser
"Moreover, gastrodin can inhibit the interaction between USP19 and survivin to stimulate the destruction and ubiquitination of survivin."

reach
"This observation supported the notion that gastrodin-induced apoptosis in NSCLC cells by interfering with the binding of USP19 to survivin."

reach
"To assess the mechanism of this possible interaction between USP19 and survivin, we next treated HCC827 cells with MK2206, an AKT activity inhibitor, finding that the binding of USP19 to survivin was [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Survivin T34A mutant impaired the binding of USP19 to survivin in the presence of gastrodin ( Fig. 4 G)."
USP19 binds BIRC5 and A549. 1 / 1
| 1

reach
"Therefore, we first verified the potential effects of gastrodin on the interaction between USP19 and survivin in A549 cells."
| 13 4
| 10 4

reach
"USP19 selectively suppresses pTh17 cell differentiation in vitro.."

reach
"USP19 can inhibit myogenic differentiation through suppression of an unfolded protein response that is required for muscle cell fusion 74."

reach
"Consistent with these findings, the deubiquitinase USP19 suppressed pTh17 differentiation in vitro as well as Th17-mediated pathogenesis in vivo."

reach
"In the case of USP19, we have also provided evidence that this modulation of UPR signaling may also occur in vivo during myogenic regeneration in response to muscle injury and that loss of USP19 can lead to a more rapid differentiation and larger myofibers (XREF_FIG)."

reach
"Because USP19 inhibits differentiation, it was somewhat surprising to observe its induction during differentiation."

reach
"Consistent with this finding, the deubiquitinase USP19 was shown to suppress pTh17 differentiation in vitro and Th17-mediated pathogenesis in vivo."

reach
"Consistent with these findings, a deubiquitinase USP19 suppressed pTh17 differentiation in vitro as well as Th17-mediated pathogenesis in murine autoimmune models."

sparser
"USP19-ER inhibited both muscle cell differentiation and the unfolded protein response gene CHOP that occurs during differentiation."

reach
"USP19 deubiquitinating enzyme inhibits muscle cell differentiation by suppressing unfolded protein response signaling."

reach
"Consistent with this, our recent studies also demonstrate that overexpression of USP19 can impair muscle cell differentiation in vitro (unpublished data)."
| 3

reach
"We therefore tested whether USP19 modulates this differentiation dependent CHOP induction and, if so, whether it requires ER localization."

reach
"These results suggested that USP19 inhibits muscle cell differentiation by suppressing the differentiation dependent induction of the UPR during myogenesis."

reach
"Our previous observations that silencing USP19 induced expression of myofibrillar proteins as well as myogenin in muscle cells suggested that USP19 might modulate muscle cell differentiation."
USP19 affects YAP1
| 13 2
USP19 increases the amount of YAP1.
| 4
USP19 increases the amount of YAP1. 4 / 4
| 4

reach
"The results showed that knockdown USP19 considerably decrease YAP protein expression in Fig. S1 ."

reach
"Taken together, knockdown of USP19 largely decreased the expression of YAP protein, which could be abolished by restoring YAP expression ( Fig. 7 E)."

reach
"USP19 knockdown significantly decreased the expression of YAP protein via proteasome pathway in a dose-dependent manner."

reach
"The result of WB and qPCR indicated that USP19 depletion decreased the expression of YAP protein and its target genes ( CTGF, CYR61 and ANKRD1 ) ( Fig. 1 B and C)."
USP19 binds YAP1.
| 2 2
| 2 2

reach
"Besides, the nuclear and cytoplasmic separation CoIP assay indicated that the interaction between USP19 and YAP occurred in the cytoplasm ( Fig. 3 C)."

sparser
"Moreover, the data from TCGA database displayed that USP19 was positively associated with YAP1 and its target genes ( CTGF and CYR61 ) ( Fig. 1 J, Fig. S2E and F )."

reach
"To further elucidated the cooperation between USP19 and YAP, we constructed the deletion construction of them ( Fig. 2 D)."

sparser
"Besides, the nuclear and cytoplasmic separation CoIP assay indicated that the interaction between USP19 and YAP occurred in the cytoplasm ( Fig. 3 C)."
USP19 decreases the amount of YAP1.
| 3
USP19 decreases the amount of YAP1. 3 / 3
| 3

reach
"Taken together, knockdown of USP19 largely decreased the expression of YAP protein, which could be abolished by restoring YAP expression ( Fig. 7 E)."

reach
"The trans -well and wound healing experiments indicated that the migration rate of USP19 depletion cells could be reversed by increasing the expression of YAP ( Fig. 7 A and B)."

reach
"The CCK8 assay also proved that suppressive function induced by USP19 knockdown restored by upregulating YAP expression ( Fig. 7 D)."
USP19 activates YAP1.
| 3
USP19 activates YAP1. 3 / 3
| 3

reach
"Besides, ectopic expression of USP19 could increase YAP protein in a dose-dependent manner, while USP19 C506S lost the ability to increase YAP protein ( Fig. 1 G)."

reach
"For instance, the E3 ubiquitin ligases Fbxw7 and β-TrCP have been implicated in YAP1 ubiquitination and degradation in HCC [13, 14], while the deubiquitinase USP19 enhances YAP1 stability [15]."

reach
"Furthermore, as mentioned earlier, USP19 has a diverse range of substrates, and its activity is also influenced by multiple signaling pathways, highlighting its involvement in complex cellular process[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP19 deubiquitinates YAP1.
| 1
USP19 leads to the deubiquitination of YAP1 on K90. 1 / 1
| 1

reach
"It was found that USP19 increased the deubiquitylated of YAP at K76 and K90 sites ( Fig. 3 F)."
BECN1 affects USP19
1 | 4 10
1 | 4 10

sparser
"Thus, an immunoprecipitation experiment was conducted to provide additional evidence for the potential interaction between USP19 and Beclin1."

sparser
"Resveratrol (Res), a polyphenol with multiple bioactivities, was demonstrated to diminish oxidative stress and Fe 2+ levels in I/R models and regulate USP19-Beclin 1 autophagy to inhibit ferroptosis [ xref ]."

sparser
"Beclin1 was detected among the proteins precipitated using USP19, suggesting an interaction between Beclin1 and USP19 (Fig. xref )."

No evidence text available

sparser
"The Beclin 1-ubiquitin-specific protease 19 (BECN1USP19) axis, a positive regulator of autophagy initiation and progression, plays a role in the crosstalk between autophagy and retinoic acid-inducible gene I (RIG-I) -mediated type I interferon signaling [ xref ]."

sparser
"The IP results in this study showed that USP19 interacts with the Beclin1 protein to initiate autophagy in cells."

reach
"In addition, co-immunoprecipitation showed an interaction between USP19 and Beclin1."

reach
"Thus, an immunoprecipitation experiment was conducted to provide additional evidence for the potential interaction between USP19 and Beclin1."

reach
"Beclin1 was detected among the proteins precipitated using USP19, suggesting an interaction between Beclin1 and USP19 (Fig. 5B)."

reach
"Both phenomena indicated that the interaction between USP19 and Beclin1 induced by TIPE3 promoted cellular autophagy.M2-type macrophages, also known as anti-inflammatory and reparative macrophages, typically play a role in tissue repair, healing, and tumor microenvironment [29]."
USP19 affects autophagy
| 14
USP19 activates autophagy.
| 11
| 11

reach
"For example, USP6/TRE17 is involved in activation of NF-κB pathway [ 24 , 25 ]; USP8 regulates T cell homeostasis and mitophagy in mitochondria [ 26 , 27 ]; USP10 or USP42 regulates p53 localization a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"In addition, USP19 induces autophagy through deubiquitination of beclin1 [42]."

reach
"The IP results in this study showed that USP19 interacts with the Beclin1 protein to initiate autophagy in cells."

reach
"The researchers found that USP19 promoted autophagy and thus downregulated NLRP3 inflammasome activation."

reach
"TIPE3 upregulates the expression of USP19, enhancing autophagy."

reach
"Interaction of USP19 and Beclin1 induced by TIPE3 promotes cellular autophagy."

reach
"Consistent with this finding, a study has shown that the deubiquitinase USP19 stabilizes Beclin-1 by removing the K11-linked ubiquitin chains from Beclin-1, and positively regulates autophagy ."

reach
"By removing K11 linked chains at K437 from Beclin-1, an essential factor of autophagic initiation, USP19 induces autophagy and inhibits the production of type I IFN [XREF_BIBR]."

reach
"Resveratrol also inhibits autophagy, mediated by ubiquitin-specific peptidase 19 (USP19)-Beclin1, to minimise ferroptosis (102)."

reach
"USP19 positively regulates autophagy by stabilizing Beclin-1, an essential autophagy protein, via deubiquitination [27]."
| PMC
USP19 inhibits autophagy.
| 3
| 3

reach
"We found that levels of p62 reduced as processed LC3B-II levels increased in USP19KO cells, suggesting that loss of USP19 could lead to altered autophagy (Fig. 2, E and F)."

reach
"USP19 inhibited NLRP3 inflammasome activation by increasing autophagy flux and decreasing the generation of mitochondrial reactive oxygen species."

reach
"We next investigated whether USP19 loss could lead to altered lysosomal function and/or autophagy."
USP19 affects HSP90
| 6 7
USP19 binds HSP90.
| 5 7
| 5 7

sparser
"It is the first DUB reported to be regulated by heat shock protein, Hsp90, which binds with the catalytic domain of USP19 and promotes its substrate association."

reach
"Biochemical study showed that USP19 binds to two major heat shock proteins HSC70 and HSP90 in cells, suggesting a possible role in PQC (Lee et al., 2014)."

sparser
"The interaction of USP19 with HSP90 accelerates the aggregation of HTT by modulating the protein level of HTT, confirming that the effect of USP19 decreases when the interaction between HTT and HSP90 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Indeed, an interaction of USP19 with Hsp90 has been described ( He et al., 2016; Lee et al., 2014 )."

sparser
"Collectively, the cytoplasmic USP19 associates with HSP90 potentially forming a dynamic complex in cells, which may function in quality control for the polyQ-expanded proteins."

sparser
"Both forms of USP19 specifically interact with HSP90 through their N-terminal CS domains and then associate with CHIP via HSP90."

sparser
"The regulatory function of USP19 was recently confirmed in a study demonstrating that USP19 interacts directly with chaperone Hsp90 and upregulates the aggregation of poly-Q containing the proteins Ataxin-3 and Huntingtin, which causes spinocerebellar ataxia type-3 and Huntington’s disease, respectively [ xref ]."

reach
"Thus, HSP90 interacts with Htt-N90 on the N-terminal amphipathic alpha-helix, and then recruits USP19 to modulate the protein level and aggregation of Htt-N90."

reach
"HSP90 interacts with the N-terminus of HTT and recruits USP19, thereby affecting the expression level and aggregation of HTT ( He et al., 2017 )."

reach
"However, when the TM domain was removed, the C-terminal (CT) fragment of USP19 could now bind Hsp90 almost as efficiently as full-length WT USP19."
USP19 activates HSP90.
| 1
USP19 activates HSP90. 1 / 1
| 1

reach
"6 , 7 Through interacting with HSP90 via the CS/p23 domains, USP19 may promote HSP90 folding by giving it the ability to function as an independent partner."
| 1 9 1
| 1 6

reach
"The results showed that BAG6 knockdown effectively reversed the promotion of proliferation and suppression of apoptosis induced by USP19 overexpression (Figure 5A–F)."

reach
"The expression level of USP19 remained unchanged after overexpressing or knocking down the BAG6 protein, indicating that BAG6 had no impact on the protein level of USP19 (Figure 6L).3.7 USP19 promoted apoptosis through BAG6-induced ER stress in human BC cells."

reach
"USP19 knockdown attenuated HG+FFA-triggered growth inhibition and apoptosis promotion in HK-2 cells."

reach
"We also found that USP19 overexpression inhibits cell proliferation, induces apoptosis, and regulates cell‐cycle assay in TNBC cells."

reach
"USP19 was significantly regulated in MDA‐MB‐231 compared with the levels detected in the control groups (Figure S1F).3.2 USP19 suppressed cell proliferation and induced apoptosis of BC cell lines."

eidos
"In CSE-treated BEAS-2B cells , USP19 knockdown reduced FUNDC1 and LC3 II / I , increased the levels of TOM20 , improved cell survival , mitochondrial membrane potential , and OCR , and inhibited apoptosis ."

reach
"We demonstrated that expression of USP19 inhibited proliferation and induced apoptosis in TNBC cells, as well as promoting BAG6 protein expression by deubiquitinating modification."
| 3 1

sparser
"It has been also reported that USP19 inhibits TNFα -induced apoptosis via the stabilization of c-IAP s, which are ubiquitin ligases that regulate the stability of a variety of apoptotic and non-apoptotic proteins [ xref ]."

reach
"Furthermore, the flow cytometric analysis demonstrated that the knockdown of USP19 could promote Hep3B and Huh7 cells apoptosis ( Fig. 5 F)."

reach
"Next, overexpression of USP19 markedly reduced the apoptosis rate of cells compared to that in the L-OHP group (Fig. 4H, I)."

reach
"Among the additional 26 genes, we noted that module 28 included USP19 and LRRFIP2, which can inhibit apoptosis or pyroptosis and were higher in HIV-DNA cells, and TLN1 , which is required for antigen-driven T cell proliferation mediated through immunological synapses and was also higher in HIV-DNA cells."
USP19 affects MARCHF6
1 | 9 1
USP19 binds MARCHF6.
| 2 1
| 2 1

reach
"Since transmembrane USP19 and MARCH6 have been shown to be localized to the ER, we first assessed the possibility that USP19 interacts with MARCH6."

sparser
"Since transmembrane USP19 and MARCH6 have been shown to be localized to the ER, we first assessed the possibility that USP19 interacts with MARCH6."

reach
"We found that USP19 also interacts with and stabilizes MARCH6."
USP19 increases the amount of MARCHF6.
| 3
USP19 increases the amount of MARCHF6. 3 / 3
| 3

reach
"Since enzyme activity is generally proportional to the protein expression levels, we expected that knockdown of USP19 would reduce MARCH6 expression, which in turn would stabilize the ABCB11 G238V mut[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"In support of this hypothesis, we showed that ABCB11 G238V was stabilized by the USP19 knockdown that reduced MARCH6 expression."

reach
"Furthermore, we addressed whether knockdown of the endogenous USP19 protein with small interfering RNA (siRNA) would reduce the levels of endogenous MARCH6."
USP19 deubiquitinates MARCHF6.
1 | 1
USP19 deubiquitinates MARCHF6. 2 / 2
1 | 1

reach
"In this study, we show that USP19 deubiquitinates and stabilizes MARCH6."
USP19 activates MARCHF6.
| 2
USP19 activates MARCHF6. 2 / 2
| 2

reach
"These results suggest that USP19 is likely to positively regulate the MARCH6 mediated ERAD of ABCB11 G238V."

reach
"Further investigation is needed to elucidate the physiological significance of USP19 mediated stabilization of MARCH6, and also to identify novel substrates and regulatory factors."
USP19 ubiquitinates MARCHF6.
| 1
USP19 ubiquitinates MARCHF6. 1 / 1
| 1

reach
"Reduced USP19 expression resulted in an increase in ubiquitination of FLAG-MARCH6."
USP19 affects HDAC1
2 | 4 3
2 | 4 1

No evidence text available

reach
"Immunoblot analysis showed that both HDAC1 and HDAC2 could bind to USP19."

sparser
"Interestingly, as shown in Figure xref and Figure xref , we found much stronger binding of USP19 to HDAC1 in response to IR irradiation, further suggesting the HDAC-USP19 complex was involved in DNA damage response."

No evidence text available

reach
"Interestingly, as shown in Figure XREF_FIG and Figure XREF_FIG, we found much stronger binding of USP19 to HDAC1 in response to IR irradiation, further suggesting the HDAC and USP19 complex was involved in DNA damage response."

reach
"USP19 interacts with HDAC1 and HDAC2."

reach
"Of all the binding proteins, we found USP19 could interact with HDAC1 and HDAC2, which have been reported to play important roles in DNA damage repair."
| 2

sparser
"USP19 interacts with HDAC1 and HDAC2."

sparser
"Immunoblot analysis showed that both HDAC1 and HDAC2 could bind to USP19 (Figure xref and xref )."
SIAH1 affects USP19
7 | 4 1
SIAH1 binds USP19.
7 | 2 1
7 | 2 1

No evidence text available

reach
"To assess whether USP19 interacts with SIAH1 and SIAH2 in eukaryotic cells, HEK293T cells were co-transfected with plasmids expressing Myc-USP19 and HA-SIAH1 or HA-SIAH2 and cell lysates were subjecte[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Probing Western blots of anti-Myc immunoprecipitates with an anti-HA antibody confirmed that USP19 interacts with both SIAH1 and SIAH2."

No evidence text available

No evidence text available

sparser
"In the early stages of virus infection, stabilized SIAH1 directly interacts with the USP19 and simultaneously mediates K27-linked ubiquitination of 489, 490, and 610 residues of USP19 for proteasomal degradation."

No evidence text available

No evidence text available

No evidence text available

No evidence text available
SIAH1 ubiquitinates USP19.
| 1
SIAH1 leads to the ubiquitination of USP19. 1 / 1
| 1

reach
"Here we report that USP19 interacts with the ubiquitin ligases SIAH1 and SIAH2, which promote USP19 ubiquitylation and degradation by the proteasome."
SIAH1 inhibits USP19.
| 1
SIAH1 inhibits USP19. 1 / 1
| 1

reach
"The expression levels of Myc-USP19 Delta462-473 remained constant in spite of SIAH overexpression while Myc-USP19 was efficiently degraded, confirming that the SIAH binding motif is required for USP19[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
MGMT affects USP19
1 | 3 8
1 | 3 8

sparser
"Furthermore, our results show that USP19MGMT axis related to the MGMT‐positive GBM cell response to TMZ treatment."

sparser
"To gain more proof to certify the biological function of USP19MGMT axis in GBM cells chemo‐resistance in vivo, we employed shRNA to specifically knockdown USP19 in T98G cells, and nude mice were subcutaneously implanted with T98G USP19 depletion cells and control (Ctrl) cells."

reach
"Mechanistically, USP19 binds to MGMT and then deubiquitinates MGMT, causing the removal of ubiquitin chains, which stabilize MGMT."

reach
"Additionally, endogenous immunoprecipitation further confirmed that MGMT binds to USP19 (Figure 2B)."

reach
"To further investigate the mechanism of binding between USP19 and MGMT, we found that the N terminal (1‐596aa) and UI domain of USP19 were responsible for binding with MGMT (Figure 2C).2.3 USP19 deubiquitinates MGMT in vivo and in vitro."

sparser
"Since USP19MGMT axis regulates the sensitivity of GBM to TMZ, we analyzed the overall survival of GBM patients in different USP19 expression backgrounds."

sparser
"Mechanistically, USP19 binds to MGMT and then deubiquitinates MGMT, causing the removal of ubiquitin chains, which stabilize MGMT."

sparser
"In this study, we found that the DNA O6‐methylation lesions repair is dependent on the USP19MGMT axis, which is a new perspective to intervene in the mechanism of alkylation lesions repair, especially the TMZ treatment for GBM patients."

sparser
"Our study reports the potential of USP19MGMT axis as a new chemotherapy target and biomarker in GBM patients."

No evidence text available
ME1 affects USP19
3 | 2 7
3 | 2 7

sparser
"Next, we studied whether ERK2 affected the interaction between ME1 and USP19."

sparser
"IP results showed that EGF treatment dramatically enhanced USP19-ME1 interaction in NCM460 cells ( Figure S2 R)."

reach
"The endogenous interactions between USP19 and ME1 were confirmed by co-IP experiments in CRC HCT116 and LoVo cells ( Figures 1 B and S1 B)."

reach
"Next, we studied whether ERK2 affected the interaction between ME1 and USP19."

sparser
"These results are consistent with the proteomic and biochemical findings, discussed above, and support the idea that the USP19-ME1 axis mediates lipogenesis and plays a critical role in both murine an[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Finally, the authors showed that the USP19-ME1 signaling axis is dysregulated in human colorectal cancer samples, and that USP19 is upregulated during colorectal carcinogenesis pathogenesis and spontaneous tumor development."

No evidence text available

No evidence text available

No evidence text available

sparser
"The endogenous interactions between USP19 and ME1 were confirmed by co-IP experiments in CRC HCT116 and LoVo cells ( Figures 1 B and S1 B)."
HSP90 affects USP19
| 5 7
| 5 7

sparser
"It is the first DUB reported to be regulated by heat shock protein, Hsp90, which binds with the catalytic domain of USP19 and promotes its substrate association."

reach
"Biochemical study showed that USP19 binds to two major heat shock proteins HSC70 and HSP90 in cells, suggesting a possible role in PQC (Lee et al., 2014)."

sparser
"The interaction of USP19 with HSP90 accelerates the aggregation of HTT by modulating the protein level of HTT, confirming that the effect of USP19 decreases when the interaction between HTT and HSP90 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Indeed, an interaction of USP19 with Hsp90 has been described ( He et al., 2016; Lee et al., 2014 )."

sparser
"Collectively, the cytoplasmic USP19 associates with HSP90 potentially forming a dynamic complex in cells, which may function in quality control for the polyQ-expanded proteins."

sparser
"Both forms of USP19 specifically interact with HSP90 through their N-terminal CS domains and then associate with CHIP via HSP90."

sparser
"The regulatory function of USP19 was recently confirmed in a study demonstrating that USP19 interacts directly with chaperone Hsp90 and upregulates the aggregation of poly-Q containing the proteins Ataxin-3 and Huntingtin, which causes spinocerebellar ataxia type-3 and Huntington’s disease, respectively [ xref ]."

reach
"Thus, HSP90 interacts with Htt-N90 on the N-terminal amphipathic alpha-helix, and then recruits USP19 to modulate the protein level and aggregation of Htt-N90."

reach
"HSP90 interacts with the N-terminus of HTT and recruits USP19, thereby affecting the expression level and aggregation of HTT ( He et al., 2017 )."

reach
"However, when the TM domain was removed, the C-terminal (CT) fragment of USP19 could now bind Hsp90 almost as efficiently as full-length WT USP19."
HDAC1 affects USP19
2 | 4 3
2 | 4 1

No evidence text available

reach
"Immunoblot analysis showed that both HDAC1 and HDAC2 could bind to USP19."

sparser
"Interestingly, as shown in Figure xref and Figure xref , we found much stronger binding of USP19 to HDAC1 in response to IR irradiation, further suggesting the HDAC-USP19 complex was involved in DNA damage response."

No evidence text available

reach
"Interestingly, as shown in Figure XREF_FIG and Figure XREF_FIG, we found much stronger binding of USP19 to HDAC1 in response to IR irradiation, further suggesting the HDAC and USP19 complex was involved in DNA damage response."

reach
"USP19 interacts with HDAC1 and HDAC2."

reach
"Of all the binding proteins, we found USP19 could interact with HDAC1 and HDAC2, which have been reported to play important roles in DNA damage repair."
| 2

sparser
"USP19 interacts with HDAC1 and HDAC2."

sparser
"Immunoblot analysis showed that both HDAC1 and HDAC2 could bind to USP19 (Figure xref and xref )."
BAG6 affects USP19
6 | 3 3
6 | 3 3

reach
"We also have conducted an IP/WB assay to showcase the interaction between USP19 and BAG6 in both breast normal and TNBC cell lines (MCF‐10A and MDA‐MB‐231) (Figure 3I,J)."

reach
"15 Here, we showed that USP19 binds to BAG6 and decreases BAG6 ubiquitination, which prevents BAG6 from being degraded in TNBC tumour tissue and BC cells."

No evidence text available

No evidence text available

sparser
"Next, we examined the binding association of BAG6 and USP19 in cells by IP‐Western blot analysis."

No evidence text available

No evidence text available

sparser
"We also have conducted an IP/WB assay to showcase the interaction between USP19 and BAG6 in both breast normal and TNBC cell lines (MCF‐10A and MDA‐MB‐231) (Figure  xref )."

sparser
"We also explored whether BAG6 interacts with USP19 mRNA directly."

No evidence text available
USP19 affects TBK1
2 | 9
USP19 inhibits TBK1.
| 6
USP19 inhibits TBK1. 6 / 6
| 6

reach
"Here, we demonstrate that USP19 (ubiquitin specific peptidase 19) interacts with and promotes TBK1 lysosomal degradation via chaperone-mediated autophagy (CMA)."

reach
"S4D), suggesting VANGL2-induced degradation of TBK1 to inhibit IFN-I signaling.Most literature showed that the degradation of TBK1 is relied on the proteasome, except for a few recent studies that indicated that USP19, NEDD4, and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nonstructural protein 13 (NSP13) could induce the autophagic degradation of TBK1 (7, 25, 26)."

reach
"Furthermore, USP19 deficiency in macrophages caused an elevation of TBK1 and the activation of the type-I interferon signaling pathway after vesicular stomatitis virus (VSV) infection."

reach
"Mechanistically, USP19 promoted the degradation of TBK1 in a lysosome-dependent manner, which serves an important role in the innate immune response against the virus [ 54 ]."

reach
"USP19 promotes the degradation of TBK1 through chaperone-mediated autophagy [ 36 ]."

reach
"51 It has been reported further that USP19 promotes TBK1 degradation through chaperone-mediated autophagy.52 Severe acute respiratory syndrome coronavirus 2 M protein also interacts with TBK1 and induces TBK1 degradation by K48-linked ubiquitination."
USP19 binds TBK1.
2 |
2 |

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USP19 activates TBK1.
| 2
USP19 activates TBK1. 2 / 2
| 2

reach
"USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy."

reach
"We observed that TBK1 had a canonical CMA motif, knocking down key proteins involved in CMA (HSPA8/HSC70 or LAMP2A) or inhibiting CMA-prevented USP19-mediated TBK1 degradation."
USP19 increases the amount of TBK1.
| 1
USP19 increases the amount of TBK1. 1 / 1
| 1

reach
"Down-regulation of USP19 increased the phosphorylation levels of TBK1 and IRF3 and inhibited autophagy induced by IAV infection due to the ubiquitination of Beclin1[129], indicating that Cyanidin and C3G showed anti-influenza virus activity against IAV infection by regulating USP19-Beclin1-dependent autophagy."
USP19 affects Neoplasms
| 1 10
| 1 10

reach
"In vivo experiments showed that USP19 silencing reduces tumorigenicity and delays tumor onset and growth, and the opposite was observed upon wild type USP19 overexpression (but not when overexpressing a catalytically dead mutant, or a cytoplasmic version of USP19)."

reach
"Moreover, the downregulation of USP19 promoted tumor growth in a xenograft model."
| PMC

eidos
"USP19 Negatively Regulates p53 and Promotes Cervical Cancer Progression ."

reach
"Also, worth investigating is whether USP19 inhibitors are promising drugs against tumors in general, or only some specific types of tumors, like liver cancers with p53 deletions/mutations that originate in a NAFLD background.In conclusion, this study shows that p53 deletion/mutation increases USP19, which in turn stabilizes SOAT1, increases cholesterol esterification and accelerates tumor progression (Figure 1)."

reach
"USP19 depletion significantly diminished the tumor weight and tumor volume compared with shControl group ( Fig. 6 A–D)."

reach
"Furthermore, the authors of this study also showed that USP19 depletion decreased tumor growth and metastasis in vivo."

reach
"Therefore, anti-tumor or pro-tumor effects mediated by USP19-CY may differ depending on cancer subtype."

reach
"Additionally, USP19 depletion results in significant enrichment of apoptosis and reduces the growth of tumors in the mouse xenograft."

reach
"The results showed that USP19 knockdown suppressed the proliferation, anchorage-independent growth and xenograft tumor formation of DLBCL cells and arrested the cell cycle at the G1 stage."

reach
"They validated their results using in vivo models and observed that USP19 downregulation promoted tumor growth in a xenograft model."
| 1 10

reach
"We demonstrated that USP19 positively regulates breast tumor cells migration and invasion in vitro, and that genetic silencing reduces cells motility, whereas its overexpression increases migratory and invasive capabilities—dependent on USP19’s catalytic activity and ER localization."

eidos
"USP19 modulates cancer cell migration and invasion and acts as a novel prognostic marker in patients with early breast cancer Tumor cell dissemination in cancer patients is associated with a significant reduction in their survival and quality of life ."

reach
"Transwell assay showed that knockdown of USP19 decreased invasiveness of SGC7901 cells, whereas overexpression of USP19 enhanced GES1 cell invasion (P < 0.01; XREF_FIG and XREF_FIG, respectively)."

reach
"In our study, the cell invasion and migration induced by USP19 was correlated with MMP2 and MMP9 expression and enzyme activity."

reach
"USP19 modulates cancer cell migration and invasion and acts as a novel prognostic marker in patients with early breast cancer."

reach
"In contrast, overexpression of USP19 increased cell invasiveness both in vitro and in vivo, further validating our findings."

reach
"USP19 knockdown impairs invasion."

reach
"Collectively, our results indicate that USP19 knockdown inhibits tumor cell invasion in vitro."

reach
"USP19 overexpression enhances migration and invasion."

reach
"In accordance with our previous results, the USP19 dependent increase in invasion is also determined by its catalytic activity and presence of the transmembrane domain."
USP19 affects MAP3K7
2 | 7 2
USP19 binds MAP3K7.
2 | 1 2
2 | 1 2

No evidence text available

sparser
"Recently, our group found that USP19 is associated with TAK1 in a TNFα- or IL-1β-dependent manner and specifically deconjugates K63- and K27-linked polyubiquitin chains from TAK1, leading to the impairment of TAK1 activity and the disruption of TAK1-TAB2/3 complex."

reach
"The USP19/TAK1 interaction and ubiquitinated TAK1 levels were assayed by coimmunoprecipitation (Co-IP) assays and IB."

sparser
"Mechanistically, USP19 interacted with TAK1 in a TNF-α- or IL-1β-dependent manner and specifically deconjugated K63- and K27-linked polyubiquitin chains from TAK1, leading to the impairment of TAK1 activity and the disruption of the TAK1-TAB2/3 complex."

No evidence text available
USP19 ubiquitinates MAP3K7.
| 2
USP19 leads to the ubiquitination of MAP3K7. 2 / 2
| 2

reach
"Saito and Tanaka (51) revealed that inhibiting the Notch and NF-κB pathway prevents the progression of OA, whilst Lei et al (52) observed that ubiquitin specific peptidase 19 suppresses TNF-α and IL-1β-induced NF-κB activation by deubiquitinating mitogen-activated protein kinase kinase kinase 7."

reach
"USP19 Inhibits TNF-alpha- and IL-1beta-Triggered NF-kappaB Activation by Deubiquitinating TAK1."
USP19 inhibits MAP3K7.
| 2
USP19 inhibits MAP3K7. 2 / 2
| 2

reach
"USP19 negatively regulates the activation of TAK1-TAB1 dependent NF-κB signaling pathway by specifically removing TAK1 coupled Lys63 and Lys27 linked polyubiquitin chains, resulting in impaired TAK1 activity and destruction of the TAK1-TAB2/3 complex (207)."

reach
"For example, USP19 inhibits Tnf and Il1b-triggered NF-κB activation by targeting TAK1 uncoupling of k27 and k63-linked polyubiquitin chains (35)."
USP19 deubiquitinates MAP3K7.
| 2
USP19 deubiquitinates MAP3K7. 2 / 2
| 2

reach
"These observations suggest that USP18 and USP19 deubiquitinate TAK1 in a cell type specific dependent manner."

reach
"USP19, which catalyzes the hydrolysis of ubiquitin from its ubiquitin-conjugated protein substrates, is widely expressed in a variety of tissues, including liver, small intestine, and colon ( Combaret[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP19 affects LRP6
1 | 8 1
USP19 deubiquitinates LRP6.
1 | 5
USP19 deubiquitinates LRP6. 6 / 6
1 | 5

reach
"Co-receptor LRP6 is deubiquitinated by USP19 to regulate its protein maturation and expression on the cell surface, whereas its ubiquitin ligases remained unidentified (Perrody et al. 2016)."

reach
"Upon release from this putative ubiquitin binding chaperone, LRP6 is deubiquitinated by USP19."

"We show that folding of the Wnt signaling coreceptor <span class="match term1">LRP6</span> is promoted by ubiquitination of a specific lysine, retaining it in the ER while avoiding degradation. Subsequent ER exit requires removal of ubiquitin from this lysine by the deubiquitinating enzyme <span class="match term0">USP19</span>."

reach
"USP8 and USP6 constitutively deubiquitylate Fz, 65 , 66 , 67 , 68 whereas USP19 deubiquitylates LRP6 in the endoplasmic reticulum, facilitating its transport."

reach
"After the deubiquitylation of LRP6 by USP19, Wnt signaling was increased, which induced cell migration and invasion [51]."

reach
"Before ER exit, LRP6 is deubiquitinated by USP19 and palmitoylated, then transported through the Golgi to the plasma membrane where it acts as a Wnt signaling coreceptor [66,67] ."
USP19 activates LRP6.
| 2
USP19 activates LRP6. 2 / 3
| 2

reach
"Finally, these observations demonstrate that USP19 promotes the biogenesis of LRP6, with only 18% of newly synthesized LRP6 molecules surviving beyond 6 hr in the absence of USP19 and 60% surviving upon USP19 overexpression."

reach
"USP19 promotes LRP6 biogenesis and controls Wnt signaling."
USP19 binds LRP6.
| 1 1
| 1 1

reach
"Consequently, our results indicate that the functional interaction between USP19 and LRP6 is key for the regulatory effect that USP19 exerts on the modulation of breast cancer cell migration and invasion."

sparser
"Consequently, our results indicate that the functional interaction between USP19 and LRP6 is key for the regulatory effect that USP19 exerts on the modulation of breast cancer cell migration and invasion."
USP19 affects Interferon
| 11
USP19 inhibits Interferon.
| 9
| 9

reach
"Furthermore, USP19 deficiency in macrophages caused an elevation of TBK1 and the activation of the type-I interferon signaling pathway after vesicular stomatitis virus (VSV) infection."

reach
"Previously, it has been shown that USP19 negatively regulates RNA virus-triggered type I IFN induction by deubiquitinating Beclin-1 and impairing RIG-I-MAVS/VISA association in certain human cell lines ."

reach
"Previous studies have indicated that EV71 infection induces the up-regulation of USP19, which negatively regulates the antiviral type I interferon signalling by removing K-63 polyubiquitin chains from TRAF3 35."

reach
"By removing K11 linked chains at K437 from Beclin-1, an essential factor of autophagic initiation, USP19 induces autophagy and inhibits the production of type I IFN [XREF_BIBR]."

reach
"Another identified gene, USP19, negatively regulates type I interferon signaling and positively regulates the autophagy process, which are major immune cell functions previously demonstrated to be deregulated in SLE immune cells [63,64,65]."

reach
"Furthermore, USP19 negatively regulates type I interferon (IFN) signaling and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation [27,28]."
| PMC

reach
"The reduced expression of USP19 following MI and CHF could result in reduced autophagic response and could promote IFN signaling and NF-κB activation induced by tumor necrosis factor or interleukin 1β."
| PMC

reach
"USP19/ZMYND9 also negatively regulates signaling pathway of type I interferon (IFN) with dependence of Beclin-1 [149]."

reach
"USP19 suppresses cellular type I interferon signaling by targeting TRAF3 for deubiquitination."
USP19 activates Interferon.
| 2
| 2

reach
"However, USP13, USP19, and USP22 inhibit virus-induced IFN production by removing K27-linked polyubiquitin chains from STING (40, 81) or TRIF (90)."

reach
"Previously, it has been shown that USP19 negatively regulates RNA virus-triggered type I IFN induction by deubiquitinating Beclin-1 and impairing RIG-I-MAVS/VISA association in certain human cell lines ."
USP19 affects HSP90AA1
10 | 1
10 | 1

No evidence text available

No evidence text available

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reach
"HSP90AA1 interacts with the N-terminal of HTT and recruits the deubiquinating enzyme USP19 [13]."

No evidence text available

No evidence text available

No evidence text available
CORO2A affects USP19
| 5 6
| 5 4

reach
"These results show that the binding of CORO2A and USP19 was not dependent on both normal and cancer cells."

reach
"Together, our results demonstrate the importance of the interaction between USP19 and CORO2A in the regulation of RAR, which is possibly involved in adipogenesis."

sparser
"These results show that the binding of CORO2A and USP19 was not dependent on both normal and cancer cells."

sparser
"USP19 is associated with the ubiquitination of CORO2A."

sparser
"Co-immunoprecipitation assay revealed that USP19 strongly binds with CORO2A (Figure xref and xref )."

sparser
"Together, our results demonstrate the importance of the interaction between USP19 and CORO2A in the regulation of RAR, which is possibly involved in adipogenesis."

reach
"We next validated the association between USP19 and CORO2A, and the regulation of CORO2A by USP19."

reach
"Co-immunoprecipitation assay revealed that USP19 strongly binds with CORO2A."

reach
"We next evaluated the endogenous binding between CORO2A and USP19."
USP19 binds CORO2A and RAR. 2 / 2
| 2

sparser
"The results suggest that USP19 may be associated with the transcriptional regulation of RAR via CORO2A as one of the components for the NCoR complex during the adipogenesis."

sparser
"These results indicate that USP19 is associated with the regulation of transcriptional repression of RAR through CORO2A."
USP19 affects SIAH1
7 | 2 1
7 | 2 1

No evidence text available

reach
"To assess whether USP19 interacts with SIAH1 and SIAH2 in eukaryotic cells, HEK293T cells were co-transfected with plasmids expressing Myc-USP19 and HA-SIAH1 or HA-SIAH2 and cell lysates were subjecte[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Probing Western blots of anti-Myc immunoprecipitates with an anti-HA antibody confirmed that USP19 interacts with both SIAH1 and SIAH2."

No evidence text available

No evidence text available

sparser
"In the early stages of virus infection, stabilized SIAH1 directly interacts with the USP19 and simultaneously mediates K27-linked ubiquitination of 489, 490, and 610 residues of USP19 for proteasomal degradation."

No evidence text available

No evidence text available

No evidence text available

No evidence text available
SIAH2 affects USP19
4 | 4 2
SIAH2 binds USP19.
4 | 2 1
4 | 2 1

No evidence text available

reach
"To assess whether USP19 interacts with SIAH1 and SIAH2 in eukaryotic cells, HEK293T cells were co-transfected with plasmids expressing Myc-USP19 and HA-SIAH1 or HA-SIAH2 and cell lysates were subjecte[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Probing Western blots of anti-Myc immunoprecipitates with an anti-HA antibody confirmed that USP19 interacts with both SIAH1 and SIAH2."

No evidence text available

No evidence text available

No evidence text available

sparser
"SIAH2 binds to USP19 and promotes USP19 ubiquitylation and proteasome‐dependent degradation xref ; then, the hypothesis of a causal link between SIAH2 and USP19 in cardiomyocyte hypertrophy was established: Decreased transcription of SIAH2 during hypertrophic progress led to less degradation of USP19, and this mechanism partially accounted for USP19 elevation."
SIAH2 ubiquitinates USP19.
| 1 1
SIAH2 leads to the ubiquitination of USP19. 2 / 2
| 1 1

sparser
"SIAH2 binds to USP19 and promotes USP19 ubiquitylation and proteasome‐dependent degradation xref ; then, the hypothesis of a causal link between SIAH2 and USP19 in cardiomyocyte hypertrophy was established: Decreased transcription of SIAH2 during hypertrophic progress led to less degradation of USP19, and this mechanism partially accounted for USP19 elevation."

reach
"Here we report that USP19 interacts with the ubiquitin ligases SIAH1 and SIAH2, which promote USP19 ubiquitylation and degradation by the proteasome."
SIAH2 inhibits USP19.
| 1
SIAH2 inhibits USP19. 1 / 1
| 1

reach
"The expression levels of Myc-USP19 Delta462-473 remained constant in spite of SIAH overexpression while Myc-USP19 was efficiently degraded, confirming that the SIAH binding motif is required for USP19[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP19 affects UFM1
| 4 5
USP19 binds UFM1.
| 2 5
| 2 5

reach
"UFM1 interacts with and regulates USP19."

reach
"The result suggests USP19 as a rating limiting factor in UFMylation-dependent UcPS: Only in USP19 high cells, increased UFMylation can lead to more secretion, demonstrating a functional interplay between USP19 and UFM1 in UcPS.We next explored the possibility that USP19 might directly bind UFM1."

sparser
"We next explored the possibility that USP19 might directly bind UFM1."

sparser
"Pull-down using glutathione S -transferase (GST)–UFM1 fusion protein as a bait and GST as a negative control showed that USP19 CS readily coprecipitated with UFM1, whereas USP19 CD only weakly bound to UFM1 ( xref )."

sparser
"The interaction of UFM1 with USP19 further supported their coordinated action in the misfolding-associated protein secretion pathway (Wang et al., 2024a)."

sparser
"Although these methods failed to detect an interaction between USP19 ΔTM and UFM1 ( xref and fig."

sparser
"Furthermore, cross-linking–based immunoprecipitation using HEK293T cells expressing FLAG-tagged USP19 revealed an interaction between full-length USP19 and endogenous UFM1 (fig."
USP19 increases the amount of UFM1.
| 2
USP19 increases the amount of UFM1. 2 / 2
| 2

reach
"As expected, HA-UFM1 secretion is enhanced by WT USP19 but not by a USP19 mutant lacking the C-terminal transmembrane domain (ΔTM) (Fig. 5A and fig."

reach
"A USP19 catalytically inactive mutant only partially increased UFM1 secretion (fig."
USP19 affects TP53
4 | 4 1
USP19 binds TP53.
4 | 1 1
4 | 1 1

sparser
"We demonstrate a direct interaction between USP19 and p53 by pull down assay."

reach
"We demonstrate a direct interaction between USP19 and p53 by pull down assay."

No evidence text available

No evidence text available

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USP19 decreases the amount of TP53.
| 2
USP19 decreases the amount of TP53. 2 / 2
| 2

reach
"We also demonstrate that CRISPR/Cas9-mediated knockout of USP19 in cervical cancer cells elevates p53 protein levels, resulting in reduced colony formation, cell migration, and cell invasion."

reach
"Overall, our results indicate that USP19 negatively regulates p53 protein levels in cervical cancer progression."
USP19 ubiquitinates TP53.
| 1
USP19 leads to the ubiquitination of TP53. 1 / 1
| 1

reach
"The overexpression of USP19 promoted ubiquitination of p53 and reduced its protein half-life."
USP19 affects Mice
| 9
USP19 inhibits Mice.
| 5
USP19 inhibits Mice. 5 / 5
| 5

reach
"Gene knockout studies demonstrate that USP19 negatively regulates TLR3/4-mediated innate immune and inflammatory responses in cells and mice."

reach
"These data suggest that USP19 attenuates TLR3/4-mediated inflammatory responses in mice."

reach
"To our surprise, the results indicated that USP19 knockout dramatically prolonged the survival of both cohorts of mice ( Figure 3 A ) and significantly reduced AOM/DSS-induced colon tumor number, volu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Notably, USP19 overexpression reduces ferroptosis and IRI in a SLC7A11-dependent manner in mice."

reach
"Taken together with the pathological findings described above, the loss of USP19 leads to a favorable brain phenotype in PFF-injected mice, but no apparent beneficial effect on overall survival."
USP19 activates Mice.
| 4
USP19 activates Mice. 4 / 4
| 4

reach
"Loss of either SOAT1 or USP19 dramatically attenuates cholesterol esterification and hepatocarcinogenesis in the p53-deficient mice fed with either normal chow diet (NCD) or high-cholesterol, high-fat diet (HCHFD)."

reach
"Loss of USP19 reduces PD-pathology-associated neuroinflammation in mice."

reach
"The contributing ratios of daidzein and its metabolites for skeletal muscle health will be an interesting avenue for future work.In the present study, we provide evidence that the ERβ agonist daidzein[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"18 Genetic depletion of USP19 reduced Ewing sarcoma cell growth in vitro and in mice largely through destabilizing EWS–FLI1 proteins."
USP19 affects HSPA8
1 | 4 4
1 | 4 4

reach
"USP19, a previously uncovered MAPS regulator binds HSC70 and acts upstream of HSC70 and DNAJC5."

sparser
"The fact that neither the peri-nuclear localization of DNAJC5 nor its MAPS-stimulation activity depends on the J-domain raises the possibility that HSPA8 may collaborate with USP19 directly during substrate recruitment considering the reported interaction of USP19 with HSPA8 [ xref ]."

reach
"USP19 interacts with HSC70."

reach
"However, whether HSC70 interacts specifically with endogenous USP19 in untransfected cells was unclear."

sparser
"The observed interaction of USP19 with HSC70 prompted us to investigate whether DNAJC5–HSC70 uses the MAPS pathway to enhance Tau and α-Syn secretion."

sparser
"USP19 interacts with HSC70."

reach
"Biochemical study showed that USP19 binds to two major heat shock proteins HSC70 and HSP90 in cells, suggesting a possible role in PQC (Lee et al., 2014)."

sparser
"However, whether HSC70 interacts specifically with endogenous USP19 in untransfected cells was unclear."

No evidence text available
USP19 affects HIF1A
2 | 1 5
2 | 1 5

sparser
"USP19 also interacts with HIF-1α, promoting its stability and an appropriate hypoxia response ( Altun et al., 2012 )."

sparser
"Usp19 interacts with the N-terminus of HIF-1α which is also where the chaperone, Heat shock protein 90 (Hsp90), associates to stabilize HIF-1α (Fig. xref a)."

No evidence text available

No evidence text available

sparser
"USP19 interacts with HIF-1α and stabilizes HIF-1α, regardless of the ER localization and catalytic activity of USP19 in HeLa cells."

sparser
"Another DUB, USP19, was also reported to play a regulatory role in hypoxia signaling, as USP19 could interact with the basic helix-loop-helix (bHLH)-PAS domain of HIF-1α (but not HIF-2α), and with sev[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"It has been reported that USP19 interacts with HIF-1α and thereby prevents it from degradation xref ."

reach
"It has been reported that USP19 interacts with HIF-1alpha and thereby prevents it from degradation 29."
USP19 affects HDAC2
2 1 | 3 2
USP19 binds HDAC2.
2 | 3 2
2 | 3

reach
"USP19 interacts with HDAC1 and HDAC2."

No evidence text available

No evidence text available

reach
"Immunoblot analysis showed that both HDAC1 and HDAC2 could bind to USP19."

reach
"Of all the binding proteins, we found USP19 could interact with HDAC1 and HDAC2, which have been reported to play important roles in DNA damage repair."
| 2

sparser
"USP19 interacts with HDAC1 and HDAC2."

sparser
"Immunoblot analysis showed that both HDAC1 and HDAC2 could bind to USP19 (Figure xref and xref )."
USP19 deubiquitinates HDAC2.
1 |
USP19 deubiquitinates HDAC2. 1 / 1
1 |

"<span class="match term0">USP19</span> deubiquitinates <span class="match term1">HDAC1/2</span> to regulate DNA damage repair and control chromosomal stability"
USP19 affects HDAC1/2
| 7
USP19 binds HDAC1/2.
| 4
USP19 binds HDAC1/2. 4 / 6
| 4

reach
"In addition, we also detected the association between HDAC1/2 and USP19 upon DNA damage both in exogenous and endogenous station."

reach
"Based on this observation, and considering that USP19 interacts with and deubiquitinates HDAC1/2 in order to regulate DNA damage repair and chromosomal stability (Wu et al., 2017) and that both ccRCC and HGSC are characterized by high genomic instability, it is plausible that USP19-mediated deubiquitination of key regulators associated with DSB repair or genome instability might be responsible for the worse prognosis observed in ccRCC and HGSC patients with low USP19 levels (Kang et al., 2021)."

reach
"To further confirm the interaction between USP19 and HDAC1/2, we transfected Flag tagged HDAC1 or HDAC2 together with Myc tagged USP19 into HEK293T cells and co-immunoprecipitaion experiment was performed."

reach
"USP19 physically interacts with and deubiquitinates HDAC1/2 in order to regulate DNA damage repair, chromosomal stability, and tumorigenesis, and USP19 expression is low or depleted in several types of tumors [32]."
| PMC
USP19 deubiquitinates HDAC1/2.
| 3
USP19 deubiquitinates HDAC1/2. 3 / 3
| 3

reach
"Based on this observation, and considering that USP19 interacts with and deubiquitinates HDAC1/2 in order to regulate DNA damage repair and chromosomal stability (Wu et al., 2017) and that both ccRCC and HGSC are characterized by high genomic instability, it is plausible that USP19-mediated deubiquitination of key regulators associated with DSB repair or genome instability might be responsible for the worse prognosis observed in ccRCC and HGSC patients with low USP19 levels (Kang et al., 2021)."

reach
"USP19 physically interacts with and deubiquitinates HDAC1/2 in order to regulate DNA damage repair, chromosomal stability, and tumorigenesis, and USP19 expression is low or depleted in several types of tumors [32]."
| PMC

reach
"USP19 deubiquitinates HDAC1/2 to regulate DNA damage repair and control chromosomal stability."
USP19 affects EWSR1
1 | 8
USP19 binds FLI1 and EWSR1. 5 / 5
| 5

sparser
"USP19 interacts with the N-terminal domain of EWS-FLI1 and full length EWSR1."

sparser
"Most interestingly, co-expression of active USP19 with EWS-FLI1 stabilized the fusion protein whereas full length EWSR1 and FLI1 protein levels remained unchanged even though binding of USP19 to both EWS-FLI1 and full length EWSR1 was observed in immunoprecipitation experiments."

sparser
"More recently, USP19 was shown to deubiquitinate and stabilise EWS-FLI-1 fusions but not FLI-1, likely because USP19 bound to EWS-FLI-1 within N-terminal EWS sequences [ xref ]."

sparser
"It is therefore likely that EWS-FLI1 interacts with USP19 in the cytosol as the fusion protein displays a constant turnover and is shuffling in and out of the nucleus xref ."

sparser
"For this, we co-expressed myc-USP19 with 3xflag-EWS-FLI1 wild-type or mutants (K298R or K380R) and observed binding of USP19 to both EWS-FLI1 mutants, indicating that the interaction itself is independent of the presence of an ubiquitin chain (Fig.  xref )."
1 | 2

sparser
"Importantly, stabilization appears to be specific for the fusion protein as it could not be observed neither for EWSR1 nor for FLI1 wild type proteins even though USP19 binds to the N-terminal EWS region to regulate deubiquitination of both EWS-FLI1 and EWSR1."

No evidence text available

sparser
"USP19 binds to the EWSR1 domain of the fusion protein, as demonstrated by co-immunoprecipitation experiments, and reduces ubiquitination at an acceptor lysine site (the K380 residue xref ) in the FLI1 domain."
| 1

sparser
"As the main ubiquitin acceptor site for EWS-FLI1 degradation is located in the FLI1 terminus xref , we were next interested in identifying whether USP19 still interacts with EWS-FLI1 ubiquitin acceptor site mutants."
USP19 affects BIRC3
4 1 | 3
USP19 binds BIRC3.
4 | 1
4 | 1

No evidence text available

No evidence text available

No evidence text available

No evidence text available

reach
"Here we report that USP19, a deubiquitinating enzyme, interacts with cellular IAP 1 (c-IAP1) and c-IAP2."
USP19 activates BIRC3.
| 2
USP19 activates BIRC3. 2 / 2
| 2

reach
"Furthermore, DUBs are known to regulate stability of only 4 of these proteins, including the ubiquitin ligases MARCH7 and BIRC3, which are targeted by USP7 or USP9 and USP19, respectively."

reach
"For example, USP19 increased the stability of the cellular inhibitors of apoptosis 1 (c-IAP1) and c-IAP2 [XREF_BIBR]."
USP19 deubiquitinates BIRC3.
1 |
USP19 deubiquitinates BIRC3. 1 / 1
1 |

No evidence text available
TP53 affects USP19
4 | 4 1
TP53 binds USP19.
4 | 1 1
4 | 1 1

sparser
"We demonstrate a direct interaction between USP19 and p53 by pull down assay."

reach
"We demonstrate a direct interaction between USP19 and p53 by pull down assay."

No evidence text available

No evidence text available

No evidence text available

No evidence text available
TP53 inhibits USP19.
| 2
TP53 inhibits USP19. 2 / 2
| 2

reach
"Tumor suppressor P53 represses ubiquitin-specific peptidase 19 (USP19) which deubiquitinate and stabilize SOAT1, thereby inhibiting hepatocarcinogenesis [41]."

reach
"P53 also suppresses cholesterol metabolism by repressing ubiquitin-specific peptidase 19 (USP19), which deubiquitinates and stabilizes sterol O-acyltransferase (SOAT) 1 required for cholesterol esterification [360]."
TP53 decreases the amount of USP19.
| 1
TP53 decreases the amount of USP19. 1 / 1
| 1

reach
"P53 directly attenuated the transcription of the Usp19 gene by binding to its promoter [205], and the hepatocarcinogenesis induced by high-fat and high-cholesterol diets in p53-deficient mice was rescued in Usp19KO mice [205]."
SYVN1 affects USP19
2 | 2 3
2 | 2 3

sparser
"However, there was no association between Myc-USP19 and FLAG-Nixin detected ( xref B), supporting the specificity of the USP19 interaction with HRD1."

sparser
"These results suggest that USP19 interacts with HRD1."

reach
"These results suggest that USP19 interacts with HRD1."

reach
"We reported that USP19 interacts with and targets HRD1 for the deubiquitination of K48 linked ubiquitin chains, the primary signal for proteasomal degradation [XREF_BIBR, XREF_BIBR] (XREF_FIG A and XREF_FIG A)."

No evidence text available

sparser
"USP19 Interacts with HRD1."

No evidence text available
PAH affects USP19
6 | 2 1
6 | 2 1

reach
"A Duolink in situ proximity ligation assay (PLA) kit (DUO92101, Sigma-Aldrich) was used to detect the interaction between PAH and USP19 and to evaluate the ubiquitination status of PAH."

sparser
"Our study indicates that USP19 interacts with PAH and prevents polyubiquitination of PAH subsequently extending the half-life of PAH protein."

No evidence text available

No evidence text available

No evidence text available

reach
"As shown in Fig. 3b, in situ USP19 and PAH interaction was observed in the form of PLA dots when USP19 and PAHwt or the PAH variants were immunostained together (Fig. 3b, left panel), but not when they were stained with either USP19 or PAH antibody alone (Fig. 3b, right panel)."

No evidence text available

No evidence text available

No evidence text available
HSPA8 affects USP19
1 | 4 4
1 | 4 4

reach
"USP19, a previously uncovered MAPS regulator binds HSC70 and acts upstream of HSC70 and DNAJC5."

sparser
"The fact that neither the peri-nuclear localization of DNAJC5 nor its MAPS-stimulation activity depends on the J-domain raises the possibility that HSPA8 may collaborate with USP19 directly during substrate recruitment considering the reported interaction of USP19 with HSPA8 [ xref ]."

reach
"USP19 interacts with HSC70."

reach
"However, whether HSC70 interacts specifically with endogenous USP19 in untransfected cells was unclear."

sparser
"The observed interaction of USP19 with HSC70 prompted us to investigate whether DNAJC5–HSC70 uses the MAPS pathway to enhance Tau and α-Syn secretion."

sparser
"USP19 interacts with HSC70."

reach
"Biochemical study showed that USP19 binds to two major heat shock proteins HSC70 and HSP90 in cells, suggesting a possible role in PQC (Lee et al., 2014)."

sparser
"However, whether HSC70 interacts specifically with endogenous USP19 in untransfected cells was unclear."

No evidence text available
HIF1A affects USP19
2 | 1 5
2 | 1 5

sparser
"USP19 also interacts with HIF-1α, promoting its stability and an appropriate hypoxia response ( Altun et al., 2012 )."

sparser
"Usp19 interacts with the N-terminus of HIF-1α which is also where the chaperone, Heat shock protein 90 (Hsp90), associates to stabilize HIF-1α (Fig. xref a)."

No evidence text available

No evidence text available

sparser
"USP19 interacts with HIF-1α and stabilizes HIF-1α, regardless of the ER localization and catalytic activity of USP19 in HeLa cells."

sparser
"Another DUB, USP19, was also reported to play a regulatory role in hypoxia signaling, as USP19 could interact with the basic helix-loop-helix (bHLH)-PAS domain of HIF-1α (but not HIF-2α), and with sev[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"It has been reported that USP19 interacts with HIF-1α and thereby prevents it from degradation xref ."

reach
"It has been reported that USP19 interacts with HIF-1alpha and thereby prevents it from degradation 29."
EWSR1 affects USP19
1 | 8
USP19 binds FLI1 and EWSR1. 5 / 5
| 5

sparser
"USP19 interacts with the N-terminal domain of EWS-FLI1 and full length EWSR1."

sparser
"Most interestingly, co-expression of active USP19 with EWS-FLI1 stabilized the fusion protein whereas full length EWSR1 and FLI1 protein levels remained unchanged even though binding of USP19 to both EWS-FLI1 and full length EWSR1 was observed in immunoprecipitation experiments."

sparser
"More recently, USP19 was shown to deubiquitinate and stabilise EWS-FLI-1 fusions but not FLI-1, likely because USP19 bound to EWS-FLI-1 within N-terminal EWS sequences [ xref ]."

sparser
"It is therefore likely that EWS-FLI1 interacts with USP19 in the cytosol as the fusion protein displays a constant turnover and is shuffling in and out of the nucleus xref ."

sparser
"For this, we co-expressed myc-USP19 with 3xflag-EWS-FLI1 wild-type or mutants (K298R or K380R) and observed binding of USP19 to both EWS-FLI1 mutants, indicating that the interaction itself is independent of the presence of an ubiquitin chain (Fig.  xref )."
1 | 2

sparser
"Importantly, stabilization appears to be specific for the fusion protein as it could not be observed neither for EWSR1 nor for FLI1 wild type proteins even though USP19 binds to the N-terminal EWS region to regulate deubiquitination of both EWS-FLI1 and EWSR1."

No evidence text available

sparser
"USP19 binds to the EWSR1 domain of the fusion protein, as demonstrated by co-immunoprecipitation experiments, and reduces ubiquitination at an acceptor lysine site (the K380 residue xref ) in the FLI1 domain."
| 1

sparser
"As the main ubiquitin acceptor site for EWS-FLI1 degradation is located in the FLI1 terminus xref , we were next interested in identifying whether USP19 still interacts with EWS-FLI1 ubiquitin acceptor site mutants."
USP19 affects TRIM69
| 8
USP19 deubiquitinates TRIM69.
| 5
USP19 deubiquitinates TRIM69. 5 / 5
| 5

reach
"In this study, we have identified the first deubiquitinating enzyme, USP19, which mediates deubiquitination of TRIF and regulates TRIF-mediated innate immune responses.Several lines of evidence support a key role of USP19 in TRIF-mediated signaling."

reach
"USP19, which catalyzes the hydrolysis of ubiquitin from its ubiquitin-conjugated protein substrates, is widely expressed in a variety of tissues, including liver, small intestine, and colon ( Combaret[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Additionally, USP19 is implicated in the deubiquitination of TRIF, thus inhibiting TLR3/4 signaling [15]."

reach
"Deubiquitinase ubiquitin-specific protein 19 (USP19) is known to be involved in deubiquitinating Beclin1, TRAF3, and TRIF for downregulation of the type I IFN signaling."

reach
"Ultimately, we identified that SIAH1-mediated degradation of USP19 reversed USP19-mediated deubiquitination of MAVS, Beclin1, TRAF3, and TRIF, resulting in the activation of antiviral immune responses."
USP19 activates TRIM69.
| 2
USP19 activates TRIM69. 2 / 2
| 2

reach
"However, USP13, USP19, and USP22 inhibit virus-induced IFN production by removing K27-linked polyubiquitin chains from STING (40, 81) or TRIF (90)."

reach
"Previous studies have demonstrated that USP19 mediates the deubiquination of TAK1 and TRIF, thereby inhibiting TNF-α/IL-1β and TLR3/4 signaling, respectively [14,15]."
USP19 inhibits TRIM69.
| 1
USP19-C607S inhibits TRIM69. 1 / 1
| 1

reach
"Further experiments indicated that wild-type USP19 but not USP19(C607S) impaired the interaction of TRIF with TLR3 or TLR4/TRAM, whereas USP19-deficiency increased the interactions."
USP19 affects MMP9
| 8
USP19 increases the amount of MMP9.
| 5
USP19 increases the amount of MMP9. 5 / 5
| 5

reach
"We first found overexpressing USP19 increased MMP2 and MMP9 protein levels."

reach
"Furthermore, they showed that increased USP19 (TM isoform) levels enhanced MMP2/MMP9 protein expression and enzyme activity, and that genetic alteration of USP19 levels affected tumorigenesis using in vivo models."

reach
"Our study suggests that USP19 promoted tumor progression by inducing MMP2 and MMP9 expression and related enzyme activity."

reach
"Moreover, the ability of USP19 to enhance the migratory and invasive abilities of GC cells by increasing MMP2 and MMP9 expression was evaluated using gelatin zymography."

reach
"Our results first indicated that USP19 enhanced MMP2 and MMP9 protein expression as well as enzyme activity."
USP19 activates MMP9.
| 3
USP19 activates MMP9. 3 / 3
| 3

reach
"USP19, which belongs to the category of potential pro-cancer factors, enhances the MMP2/MMP9 axis and related enzyme activities in patients with gastric cancer [25]."

reach
"Multiple studies have indicated that USP19 induces the expression and enzymatic activities of MMP2 and MMP9, thereby promoting tumor progression[54]."

reach
"USP19 Enhances MMP2 and MMP9 Mediated Tumorigenesis in Gastric Cancer."
HDAC2 affects USP19
2 | 3 2
2 | 3

reach
"USP19 interacts with HDAC1 and HDAC2."

No evidence text available

No evidence text available

reach
"Immunoblot analysis showed that both HDAC1 and HDAC2 could bind to USP19."

reach
"Of all the binding proteins, we found USP19 could interact with HDAC1 and HDAC2, which have been reported to play important roles in DNA damage repair."
| 2

sparser
"USP19 interacts with HDAC1 and HDAC2."

sparser
"Immunoblot analysis showed that both HDAC1 and HDAC2 could bind to USP19 (Figure xref and xref )."
Daidzein affects USP19
| 7
Daidzein decreases the amount of USP19.
| 6
Daidzein decreases the amount of USP19. 6 / 6
| 6

reach
"Daidzein repressed USP19 expression at the mRNA and protein levels when C2C12 myotubes were cultured in the presence of daidzein ( Fig. 1 B)."

reach
"Dietary daidzein decreased the expression of USP19 at the mRNA and protein levels in soleus muscle of female mice only ( Fig. 2 B and C)."

reach
"These results indicate that in soleus muscle of OVX mice, daidzein inhibits the E2-dependent increase in USP19 expression through ERβ and the E2-depdentnt decrease in muscle ratio.To determine the eff[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Thus, these results indicate that daidzein increases ERβ recruitment to the Usp19 hERE and down-regulates USP19 expression by inhibiting E2-dependent recruitment of ERα."

reach
"The contributing ratios of daidzein and its metabolites for skeletal muscle health will be an interesting avenue for future work.In the present study, we provide evidence that the ERβ agonist daidzein[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"We demonstrate that dietary daidzein down-regulates USP19 expression and increases soleus muscle mass in young female mice.All animals in the present study were cared for in accordance with the guidel[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
Daidzein increases the amount of USP19.
| 1
Daidzein increases the amount of USP19. 1 / 1
| 1

reach
"It remains unclear whether daidzein itself or one of its metabolites contributes to the regulation of USP19 expression and muscle mass in vivo , although in C2C12 cells, daidzein itself regulates USP1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP19 affects SIAH2
4 | 2 1
4 | 2 1

No evidence text available

reach
"To assess whether USP19 interacts with SIAH1 and SIAH2 in eukaryotic cells, HEK293T cells were co-transfected with plasmids expressing Myc-USP19 and HA-SIAH1 or HA-SIAH2 and cell lysates were subjecte[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Probing Western blots of anti-Myc immunoprecipitates with an anti-HA antibody confirmed that USP19 interacts with both SIAH1 and SIAH2."

No evidence text available

No evidence text available

No evidence text available

sparser
"SIAH2 binds to USP19 and promotes USP19 ubiquitylation and proteasome‐dependent degradation xref ; then, the hypothesis of a causal link between SIAH2 and USP19 in cardiomyocyte hypertrophy was established: Decreased transcription of SIAH2 during hypertrophic progress led to less degradation of USP19, and this mechanism partially accounted for USP19 elevation."
USP19 affects MMP2
| 7
USP19 increases the amount of MMP2.
| 4
USP19 increases the amount of MMP2. 4 / 4
| 4

reach
"Moreover, the ability of USP19 to enhance the migratory and invasive abilities of GC cells by increasing MMP2 and MMP9 expression was evaluated using gelatin zymography."

reach
"We first found overexpressing USP19 increased MMP2 and MMP9 protein levels."

reach
"Our results first indicated that USP19 enhanced MMP2 and MMP9 protein expression as well as enzyme activity."

reach
"Our study suggests that USP19 promoted tumor progression by inducing MMP2 and MMP9 expression and related enzyme activity."
USP19 activates MMP2.
| 3
USP19 activates MMP2. 3 / 3
| 3

reach
"Multiple studies have indicated that USP19 induces the expression and enzymatic activities of MMP2 and MMP9, thereby promoting tumor progression[54]."

reach
"USP19 Enhances MMP2 and MMP9 Mediated Tumorigenesis in Gastric Cancer."

reach
"USP19, which belongs to the category of potential pro-cancer factors, enhances the MMP2/MMP9 axis and related enzyme activities in patients with gastric cancer [25]."
USP19 affects CDKN1B
| 6
USP19 activates CDKN1B.
| 3
USP19 activates CDKN1B. 3 / 4
| 3

reach
"Although p27 Kip1 mRNA levels remain unchanged upon USP19 depletion (XREF_FIG), it remains possible that p27 Kip1 synthesis is regulated also at the level of translation and that USP19 modulates such translational efficiency of p27 Kip1 mRNA."

reach
"Indeed, in cells transfected with USP19 siRNA p27 Kip1 levels remained relatively stable over time whereas the half-life of the protein was significantly lower in controls (XREF_FIG), implying that USP19 modulates the stability of p27 Kip1."

reach
"This increased p27 Kip1 induced by USP19 depletion did not appear to be due to increased gene transcription, as Northern blot analysis revealed that p27 Kip1 mRNA levels did not change (XREF_FIG)."
USP19 inhibits CDKN1B.
| 2
USP19 inhibits CDKN1B. 2 / 2
| 2

reach
"In their model, USP19 stabilizes KPC1 and thus indirectly promotes degradation of p27 KIP1 in the cytoplasm."

reach
"USP19 deubiquitinates and thereby stabilizes the KPC1 ligase for p27 Kip1, and so indirectly promotes degradation of p27 Kip1 and subsequent cell proliferation."
USP19 deubiquitinates CDKN1B.
| 1
USP19 deubiquitinates CDKN1B. 1 / 1
| 1

reach
"USP19 deubiquitinates and stabilizes KPC1, an E3 ligase for p27."
USP19 affects BIRC2
1 1 | 3 1
USP19 binds BIRC2.
1 | 1 1
1 | 1 1

reach
"Here we report that USP19, a deubiquitinating enzyme, interacts with cellular IAP 1 (c-IAP1) and c-IAP2."

No evidence text available

sparser
"As shown by Western Blot analysis ( xref and xref ) and epifluorescence microscopy ( xref and xref ; xref ), in contrast to control cells, USP19 expression was markedly decreased in cardiac myocytes treated with DOX, resulting in reduced protein complexes between USP19 and c-IAP1."
USP19 deubiquitinates BIRC2.
1 | 1
USP19 deubiquitinates BIRC2. 2 / 2
1 | 1

No evidence text available

reach
"USP19, constitutively de-ubiquitinates the E3 ligase activity of c-IAP1, thereby inhibiting c-IAP1’s ability to ubiquitinate and target degradation of proteins such as TRAF2 (Figure 4I, Schematic presentation)."
USP19 inhibits BIRC2.
| 1
USP19 inhibits BIRC2. 1 / 1
| 1

reach
"For example, USP19 increased the stability of the cellular inhibitors of apoptosis 1 (c-IAP1) and c-IAP2 [XREF_BIBR]."
UFM1 affects USP19
| 2 5
| 2 5

reach
"UFM1 interacts with and regulates USP19."

reach
"The result suggests USP19 as a rating limiting factor in UFMylation-dependent UcPS: Only in USP19 high cells, increased UFMylation can lead to more secretion, demonstrating a functional interplay between USP19 and UFM1 in UcPS.We next explored the possibility that USP19 might directly bind UFM1."

sparser
"We next explored the possibility that USP19 might directly bind UFM1."

sparser
"Pull-down using glutathione S -transferase (GST)–UFM1 fusion protein as a bait and GST as a negative control showed that USP19 CS readily coprecipitated with UFM1, whereas USP19 CD only weakly bound to UFM1 ( xref )."

sparser
"The interaction of UFM1 with USP19 further supported their coordinated action in the misfolding-associated protein secretion pathway (Wang et al., 2024a)."

sparser
"Although these methods failed to detect an interaction between USP19 ΔTM and UFM1 ( xref and fig."

sparser
"Furthermore, cross-linking–based immunoprecipitation using HEK293T cells expressing FLAG-tagged USP19 revealed an interaction between full-length USP19 and endogenous UFM1 (fig."
Rep affects USP19
6 |
6 |

No evidence text available
| DOI

No evidence text available
| DOI

No evidence text available

No evidence text available
| DOI

No evidence text available
| DOI

No evidence text available
| DOI
ZDHHC2 affects USP19
| 6
ZDHHC2 palmitoylates USP19.
| 3
ZDHHC2 palmitoylates USP19. 3 / 3
| 3

sparser
"ZDHHC2 is transcriptionally upregulated by a FOXA1/CXXC5/TET2 complex and promotes S‐palmitoylation of the deubiquitinase USP19, which impairs its interaction with ACSL4."

sparser
"This hypothesis was supported by co‐immunoprecipitation assays, which demonstrated that wild‐type ZDHHC2 overexpression significantly reduced the ACSL4‐USP19 protein–protein interaction (Figure  xref ; Figure  xref , Supporting Information ), but did not influence the binding between ACSL4 and the USP19 C152S mutant, which cannot be palmitoylated by ZDHHC2 (Figure  xref )."

sparser
"In brief, our results demonstrated that ZDHHC2 palmitoylates the deubiquitinase USP19, weakening the binding between USP19 and ACSL4, thereby promoting the ubiquitin‒proteasome degradation of ACSL4 (Figure  xref )."
ZDHHC2 binds USP19.
| 3
| 3

sparser
"Next, the binding region of USP19 to ZDHHC2 was analyzed via molecular docking (Figure  xref ) and GST pull‐down assays (Figure  xref ), and we found that USP19 binds to the C‐terminal region of ZDHHC2."

sparser
"Coimmunoprecipitation confirmed the endogenous interaction between ZDHHC2 and USP19 (Figure  xref ), GST pull‐down verified the direct physical interaction (Figure  xref ), and PLA further demonstrated spatial proximity interactions (Figure  xref )."

sparser
"Given that ZDHHC2 regulates ACSL4 through USP19, we subsequently investigated the functional impact of the ZDHHC2USP19 axis on ferroptosis."
| 4

reach
"For instance, USP19 inhibits the degradation of TGF-beta activated kinase 1, thereby modulating the NF-kB signaling pathway and immune responses (4)."

reach
"USP19 negatively regulates the activation of TAK1-TAB1 dependent NF-κB signaling pathway by specifically removing TAK1 coupled Lys63 and Lys27 linked polyubiquitin chains, resulting in impaired TAK1 activity and destruction of the TAK1-TAB2/3 complex (207)."

reach
"USP19/ZMYND9 also negatively regulates signaling pathway of type I interferon (IFN) with dependence of Beclin-1 [149]."

reach
"Furthermore, USP19 deficiency in macrophages caused an elevation of TBK1 and the activation of the type-I interferon signaling pathway after vesicular stomatitis virus (VSV) infection."
| 2

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"The ability of USP19 to modulate protein synthesis, degradation as well as myogenesis suggest that it is modulating a signaling pathway(s) that is upstream of these processes."

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"In a mouse model study of CRC, USP19 and ERK induced the reprogramming of lipid metabolism by regulating the USP19-ME1 signaling pathway."
USP19 affects rep
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USP19 affects calcium(2+)
| 6
USP19 increases the amount of calcium(2+).
| 5
USP19 increases the amount of calcium(2+). 5 / 5
| 5

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"Deubiquitinase USP19 modulates apoptotic calcium release and endoplasmic reticulum stress by deubiquitinating BAG6 in triple negative breast cancer."

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"Fluorescence microscopy and flow cytometry revealed a substantial rise in intracellular Ca concentration in USP19‐plasmid‐transfected cells, indicating that USP19 could enhance Ca levels (Figure 2C)."

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"Fluorescence microscopy and flow cytometry revealed a substantial rise in intracellular Ca concentration in USP19 plasmid and siBAG6 transfected cells, indicating that USP19/BAG6 axis could enhance Ca levels through inhibiting BCL2 expression (Figure 6I,J)."

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"Fluorescence microscopy revealed a substantial rise in intracellular Ca concentration in BCL2 plasmid transfected cells, indicating that BCL2 could enhance Ca levels in Figure S3E.To further clarify the regulatory role of BCL2 ubiquitination by USP19 and BAG6, MDA‐MB‐231 cells were co‐transfected with Myc‐USP19, siUSP19, Flag‐BAG6, siBAG6 and HA‐Ub."

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"Additionally, our research identified a novel mechanism by which USP19 elevates intracellular Ca levels to induce ER stress in TNBC, through regulating the ubiquitination and degradation of BAG6 and BCL2."
USP19 activates calcium(2+).
| 1
| 1

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"Our study reveals that USP19 elevates the intracellular Ca 2+ concentration to alter ER stress via regulation of BAG6 and BCL2 stability and may be a viable therapeutic target for TNBC therapy."
USP19 affects USP19
| 5
USP19 increases the amount of USP19.
| 2
USP19 increases the amount of USP19. 2 / 2
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"These results indicate that daidzein acts as an ERβ agonist and down-regulates USP19 expression in skeletal muscle cells.We previously reported that E2 promotes the recruitment of ERα to the hERE of t[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"In line with the in vitro findings, silencing of USP19 reduced the expression of USP19, FOXO1, IL-10 and IL-10R1 in mouse brain (Fig. 7A)."
USP19 decreases the amount of USP19.
| 2
USP19 decreases the amount of USP19. 1 / 1
| 1

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"Notably, we showed that herboxidiene functions as a USP19 splicing modulator by strongly decreasing the mRNA expression of USP19-CY but increasing the mRNA expression of USP19-ER, as the splicing always happens at the gene level, in breast and lung cancer cells."
Modified USP19 decreases the amount of USP19. 1 / 1
| 1

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"Overexpression of USP19 inhibited RARE transcription and knock-down of USP19 using siRNA showed dramatically increased transcription of RARE, suggesting that USP19 increases the stability of CORO2A by DUB activity, and that binding of these two protein may be associated with the function of NCoR."
USP19 activates USP19.
| 1
USP19 activates USP19. 1 / 2
| 1

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"As presented in Fig. 5A, USP19 overexpression led to induction of USP19, FOXO1, IL-10 and IL-10R1 in SH-SY5Y cells."
USP19 affects UBC
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USP19 affects RNF123
1 | 4
USP19 deubiquitinates RNF123.
1 | 2
USP19 deubiquitinates RNF123. 3 / 3
1 | 2

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"USP19 deubiquitinates and stabilizes KPC1, an E3 ligase for p27."

"<span class="match term0">USP19</span> deubiquitinates Kip1 ubiquitination-promoting complex protein 1 (<span class="match term1">KPC1</span>) regulating p27Kip1"

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"USP19 deubiquitinates and stabilizes KPC1, an E3 ligase for p27."
USP19 activates RNF123.
| 2
USP19 activates RNF123. 2 / 3
| 2

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"USP19 deubiquitinating enzyme supports cell proliferation by stabilizing KPC1, a ubiquitin ligase for p27Kip1."

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"USP19 in turn also contributes to p27 degradation by stabilizing KPC1 [70]."
USP19 affects NLRP3
| 5 1
USP19 inhibits NLRP3.
| 3
USP19 inhibits NLRP3. 3 / 3
| 3

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"USP19 inhibits inflammation and promotes M2-like macrophage polarization by regulating NLRP3 function through autophagy (15)."

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"USP19 suppresses inflammation and promotes M2 like macrophage polarization by manipulating NLRP3 function via autophagy."

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"USP19 inhibited NLRP3 inflammasome activation by increasing autophagy flux and decreasing the generation of mitochondrial reactive oxygen species."
USP19 activates NLRP3.
| 2
USP19 activates NLRP3. 2 / 2
| 2

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"In addition, USP19 inhibited the proteasomal degradation of inflammasome independent NLRP3 by cleaving its polyubiquitin chains."

reach
"Moreover, USP19 could directly promote M2 polarization by suppressing the activation of NLRP3 inflammasome to interferon regulatory factor-4 (IRF-4) (89)."
USP19 binds NLRP3.
| 1
| 1

sparser
"Therefore, this study aims to elucidate the role of USP19 in EOC and to determine whether modulating the USP19-NLRP3 signaling could represent a novel therapeutic approach for targeting TAMs in ovarian cancer."
USP19 affects NFE2L1
2 | 4
USP19 activates NFE2L1.
| 4
USP19 activates NFE2L1. 4 / 4
| 4

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"Herein, we found that Nrf1 stability and transactivity are both enhanced by USP19, a ubiquitin-specific protease tail-anchored in the endoplasmic reticulum (ER) through its C-terminal transmembrane domain."

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"Conversely, knockout of USP19 causes significant decreases in the abundance of Nrf1 and the entrance of its active isoform into the nucleus, which result in the downregulation of its target proteasomal subunits and a modest reduction in USP19 -/- -derived tumor growth in xenograft mice when compared with wild-type controls."

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"Notably, our recent work further unraveled that the deubiquitinating enzyme USP19 enables activation of its co-localized NFE2L1 in proximity to the ER and, in turn, NFE2L1 in close cooperation with NFE2L2/Nrf2 can tightly govern the transcriptional expression of USP19 and other DUBs insomuch as to form a positive feedback regulatory circuit [49]."

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"Also, USP19 activates NRF1 (encoded by Nfe2l1), thereby altering glucose deprivation sensing, cholesterol abundance, proteasomal inhibition and oxidative stress [122]."
USP19 binds NFE2L1.
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USP19 affects MYC
| 2 4
| 2 4

sparser
"The interaction between USP19 and c-Myc was measured by co-immunoprecipitation (Co-IP)."

sparser
"To further clarify the regulatory role of BCL2 ubiquitination by USP19 and BAG6, MDA‐MB‐231 cells were co‐transfected with MycUSP19, siUSP19, Flag‐BAG6, siBAG6 and HA‐Ub."

sparser
"USP19 directly bound to c-Myc, and maintained its stability by removing ubiquitination on c-Myc."

sparser
"For this, we co-expressed myc-USP19 with 3xflag-EWS-FLI1 wild-type or mutants (K298R or K380R) and observed binding of USP19 to both EWS-FLI1 mutants, indicating that the interaction itself is independent of the presence of an ubiquitin chain (Fig.  xref )."

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"The interaction between USP19 and c-Myc was measured by co-immunoprecipitation (Co-IP)."

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"USP19 directly bound to c-Myc, and maintained its stability by removing ubiquitination on c-Myc."
USP19 affects GFP1
| 6
USP19 activates GFP1.
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USP19 activates GFP1. 5 / 5
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"Like GFP, GFP1–10 secretion was enhanced by wild-type (WT) USP19, but not by the catalytically inactive USP19 mutant or a mutant lacking the TM segment (USP19 ) (Fig. 2b,d), and it was not sensitive to BFA (Supplementary Fig. 1e)."

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"We also analysed GFP1–10-containing vesicles in cells expressing different USP19 variants: WT USP19, but not USP19 or USP19 , significantly increased the number of GFP1–10 vesicles (Fig. 8b,c)."

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"As expected, overexpression of USP19 in both USP19-deficient and control cells increased GFP1–10 secretion to a similar level (Fig. 3f)."

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"Importantly, the level of membrane association correlates with the secretion efficiency: more GFP1–10 bound to the membranes than GFP in both control and USP19-overexpressing cells, and USP19 overexpression enhanced membrane interaction for both GFP and GFP1–10."

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"Moreover, USP19 depletion consistently reduced GFP1–10 in membrane fractions (Fig. 6b)."
USP19 inhibits GFP1.
| 1
USP19 inhibits GFP1. 1 / 1
| 1

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"As short interfering RNA (siRNA)-mediated downregulation of USP19 reduced GFP1–10 secretion (Fig. 3a,b), endogenous USP19 seemed to play a critical role in MAPS."
USP19 affects FLI1
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USP19 binds FLI1 and EWSR1. 5 / 5
| 5

sparser
"USP19 interacts with the N-terminal domain of EWS-FLI1 and full length EWSR1."

sparser
"Most interestingly, co-expression of active USP19 with EWS-FLI1 stabilized the fusion protein whereas full length EWSR1 and FLI1 protein levels remained unchanged even though binding of USP19 to both EWS-FLI1 and full length EWSR1 was observed in immunoprecipitation experiments."

sparser
"More recently, USP19 was shown to deubiquitinate and stabilise EWS-FLI-1 fusions but not FLI-1, likely because USP19 bound to EWS-FLI-1 within N-terminal EWS sequences [ xref ]."

sparser
"It is therefore likely that EWS-FLI1 interacts with USP19 in the cytosol as the fusion protein displays a constant turnover and is shuffling in and out of the nucleus xref ."

sparser
"For this, we co-expressed myc-USP19 with 3xflag-EWS-FLI1 wild-type or mutants (K298R or K380R) and observed binding of USP19 to both EWS-FLI1 mutants, indicating that the interaction itself is independent of the presence of an ubiquitin chain (Fig.  xref )."
| 1

sparser
"As the main ubiquitin acceptor site for EWS-FLI1 degradation is located in the FLI1 terminus xref , we were next interested in identifying whether USP19 still interacts with EWS-FLI1 ubiquitin acceptor site mutants."
UBC affects USP19
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TNFAIP8L3 affects USP19
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TNFAIP8L3 increases the amount of USP19. 6 / 6
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"In vivo experiments in mice suggested that TIPE3 inhibited apoptosis of tumor cells and promoted macrophage M2-type polarization in tumor tissues by up-regulating the protein expression of USP19."

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"Furthermore, TIPE3 upregulated USP19 protein expression, which accelerated autophagy."

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"TIPE3 upregulates the expression of USP19, enhancing autophagy."

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"Western blotting results showed that TIPE3 overexpression could upregulate the expression of USP19 following L-OHP treatment in both LoVo and SW480 cell lines (Fig. 4A)."

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"In summary, TIPE3 promotes cellular autophagy by elevating the expression of USP19/Beclin1, thereby increasing drug resistance to L-OHP in CRC cells."

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"In addition, consistent with the results of the experiments in LoVo and SW480 cells, TIPE3 also increased the protein expression level of USP19 in mouse tumor tissues (Fig. 7L, O)."
MYC affects USP19
| 2 4
| 2 4

sparser
"The interaction between USP19 and c-Myc was measured by co-immunoprecipitation (Co-IP)."

sparser
"To further clarify the regulatory role of BCL2 ubiquitination by USP19 and BAG6, MDA‐MB‐231 cells were co‐transfected with MycUSP19, siUSP19, Flag‐BAG6, siBAG6 and HA‐Ub."

sparser
"USP19 directly bound to c-Myc, and maintained its stability by removing ubiquitination on c-Myc."

sparser
"For this, we co-expressed myc-USP19 with 3xflag-EWS-FLI1 wild-type or mutants (K298R or K380R) and observed binding of USP19 to both EWS-FLI1 mutants, indicating that the interaction itself is independent of the presence of an ubiquitin chain (Fig.  xref )."

reach
"The interaction between USP19 and c-Myc was measured by co-immunoprecipitation (Co-IP)."

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"USP19 directly bound to c-Myc, and maintained its stability by removing ubiquitination on c-Myc."
HDAC1/2 affects USP19
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USP19 binds HDAC1/2. 4 / 6
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"In addition, we also detected the association between HDAC1/2 and USP19 upon DNA damage both in exogenous and endogenous station."

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"Based on this observation, and considering that USP19 interacts with and deubiquitinates HDAC1/2 in order to regulate DNA damage repair and chromosomal stability (Wu et al., 2017) and that both ccRCC and HGSC are characterized by high genomic instability, it is plausible that USP19-mediated deubiquitination of key regulators associated with DSB repair or genome instability might be responsible for the worse prognosis observed in ccRCC and HGSC patients with low USP19 levels (Kang et al., 2021)."

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"To further confirm the interaction between USP19 and HDAC1/2, we transfected Flag tagged HDAC1 or HDAC2 together with Myc tagged USP19 into HEK293T cells and co-immunoprecipitaion experiment was performed."

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"USP19 physically interacts with and deubiquitinates HDAC1/2 in order to regulate DNA damage repair, chromosomal stability, and tumorigenesis, and USP19 expression is low or depleted in several types of tumors [32]."
| PMC
FLI1 affects USP19
| 6
USP19 binds FLI1 and EWSR1. 5 / 5
| 5

sparser
"USP19 interacts with the N-terminal domain of EWS-FLI1 and full length EWSR1."

sparser
"Most interestingly, co-expression of active USP19 with EWS-FLI1 stabilized the fusion protein whereas full length EWSR1 and FLI1 protein levels remained unchanged even though binding of USP19 to both EWS-FLI1 and full length EWSR1 was observed in immunoprecipitation experiments."

sparser
"More recently, USP19 was shown to deubiquitinate and stabilise EWS-FLI-1 fusions but not FLI-1, likely because USP19 bound to EWS-FLI-1 within N-terminal EWS sequences [ xref ]."

sparser
"It is therefore likely that EWS-FLI1 interacts with USP19 in the cytosol as the fusion protein displays a constant turnover and is shuffling in and out of the nucleus xref ."

sparser
"For this, we co-expressed myc-USP19 with 3xflag-EWS-FLI1 wild-type or mutants (K298R or K380R) and observed binding of USP19 to both EWS-FLI1 mutants, indicating that the interaction itself is independent of the presence of an ubiquitin chain (Fig.  xref )."
| 1

sparser
"As the main ubiquitin acceptor site for EWS-FLI1 degradation is located in the FLI1 terminus xref , we were next interested in identifying whether USP19 still interacts with EWS-FLI1 ubiquitin acceptor site mutants."
E2F1 affects USP19
| 4 2
E2F1 increases the amount of USP19.
| 2
E2F1 increases the amount of USP19. 2 / 2
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"E2F1 promoted USP19 transcription, and increased USP19 expression counteracts the effects of E2F1 depletion on cell behaviors."

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"E2F1 activated USP19 transcription, thereby stabilizing c-Myc via deubiquitination and accelerating HCC progression."
E2F1 binds USP19.
| 1 1
| 1 1

sparser
"Dual-luciferase reporter assay and Chromatin Immunoprecipitation (ChIP) assays were used to assess the binding of E2F1 and USP19 promoter."

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"Dual-luciferase reporter assay and Chromatin Immunoprecipitation (ChIP) assays were used to assess the binding of E2F1 and USP19 promoter."
E2F1 activates USP19.
| 1 1
E2F1 activates USP19. 2 / 2
| 1 1

sparser
"E2F1 activates USP19 to affect the stability of c-Myc to facilitate the progression of hepatocellular carcinoma."

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"E2F1 activates USP19 to affect the stability of c-Myc to facilitate the progression of hepatocellular carcinoma."
BIRC3 affects USP19
4 | 1
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"Here we report that USP19, a deubiquitinating enzyme, interacts with cellular IAP 1 (c-IAP1) and c-IAP2."
| 3

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"These results suggest that overexpression of USP19 inhibits poly(I:C)- and LPS-triggered IRF3 and NF-κB activation.We next examined the role of endogenous USP19 in regulation of TLR3/4-mediated signaling."

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"Functional experiments revealed that low-dose LPS upregulated USP19 to modulate microglial M1/M2 polarization via FOXO1/IL-10/IL-10R1 signaling in SAH mice."

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"These data indicate that low-dose LPS protects against SAH-induce brain injury via upregulating USP19.3.7 Low-dose LPS upregulates USP19 to modulate microglial M1/M2 polarization via FOXO1/IL-10/IL-10R1 signaling in SAH mice."
| 1

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"Additionally, low-dose LPS counteracted SAH-decreased USP19, FOXO1, IL-10 and IL-10R1, along with SAH-increased MMP9 in mouse brain (Fig. 1H)."
Lipopolysaccharide decreases the amount of USP19.
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Lipopolysaccharide decreases the amount of USP19. 1 / 1
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"Intriguingly, SAH-decreased USP19 expression was reversed by low-dose LPS, and sh-USP19 further attenuated the effect of low-dose LPS on USP19 expression in mouse brain (Fig. 6A)."
USP19 affects α-synuclein
| 5
USP19 increases the amount of α-synuclein. 5 / 5
| 5

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"Our finding that USP19 promotes α-synuclein secretion not only suggests a physiological link between MAPS and protein-misfolding disease, but also provides a potential target for therapeutic intervention in α-synuclein propagation and α-synucleinopathies."

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"Notably, α-synuclein secretion promoted by either USP19 or DNAJC5 is mostly soluble."

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"To investigate whether MAPS is involved in cell-to-cell transmission of misfolded proteins observed in neurodegenerative diseases , we tested whether USP19 promotes the secretion of α-synuclein, a natively unfolded cytosolic protein known to be released from cells through exosomes as well as an undefined mechanism ."

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"Interestingly, WT USP19 but not USP19 or USP19 significantly stimulated α-synuclein secretion (Fig. 2h)."

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"Recently, a novel, unconventional mechanism underlying the cellular secretion of α-synuclein mediated by the ubiquitin-specific protease 19 (USP19) was identified."
USP19 affects poly(I:C)
| 5
| 3

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"Deficiency of USP19 increased serum cytokine levels after administration of poly(I:C) and LPS, and promoted inflammatory death caused by administration of poly(I:C) or LPS, or infection of Salmonella typhimurium."

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"Overexpression of USP19 inhibited poly(I:C)- and LPS-induced activation of NF-κB and IRF3, whereas USP19-deficiency had the opposite effects."

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"These results suggest that overexpression of USP19 inhibits poly(I:C)- and LPS-triggered IRF3 and NF-κB activation.We next examined the role of endogenous USP19 in regulation of TLR3/4-mediated signaling."
USP19-C607S inhibits poly(I:C). 2 / 2
| 2

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"Reconstitution of USP19 but not USP19 (C607S) into Usp19 MLFs inhibited poly(I:C)- and LPS-induced transcription of Ifnb1 and Cxcl10 (Supplementary Fig. 4b)."

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"Consistently, USP19 but not USP19(C607S) inhibited poly(I:C)- and LPS-induced activation of the IFN-β promoter in reporter assays (Supplementary Fig. 4a)."
| 5

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"In the same study, knockdown of total USP19 inhibited MDA-MB-231 cell migration [51]; this is consistent with the finding that the depletion of USP19-CY, which is the major isoform in MDA-MB-231 cells, also inhibited cell migration."

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"In contrast, USP19-CY promotes TGF-β/SMAD-induced breast and lung cancer cell EMT, cell migration and extravasation in vitro and in vivo."

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"Our results showed that USP19 positively regulated GC cell migration through the up-regulation of MMP2 and MMP9 expression and the decrease of cleaved caspase-3 activity in cell models (XREF_FIG and XREF_FIG)."

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"USP19 modulates cancer cell migration and invasion and acts as a novel prognostic marker in patients with early breast cancer."

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"These results suggest that USP19-CY promotes TGF-β-induced EMT, as well as basal and TGF-β-mediated cell migration and invasion."
USP19 affects TRAF3
1 | 4
USP19 deubiquitinates TRAF3.
1 | 3
USP19 deubiquitinates TRAF3. 4 / 4
1 | 3

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"USP19 suppresses cellular type I interferon signaling by deubiquitinating TRAF3, thereby promoting EV71 infection (4)."

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"Ultimately, we identified that SIAH1-mediated degradation of USP19 reversed USP19-mediated deubiquitination of MAVS, Beclin1, TRAF3, and TRIF, resulting in the activation of antiviral immune responses."

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"Deubiquitinase ubiquitin-specific protein 19 (USP19) is known to be involved in deubiquitinating Beclin1, TRAF3, and TRIF for downregulation of the type I IFN signaling."

"<span class="match term0">USP19</span> suppresses cellular type I interferon signaling by targeting <span class="match term1">TRAF3</span> for deubiquitination"
USP19 activates TRAF3.
| 1
USP19 activates TRAF3. 1 / 1
| 1

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"USP19 suppresses cellular type I interferon signaling by targeting TRAF3 for deubiquitination."
USP19 affects Non-structural protein 5A
5 |

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USP19 affects MYH
| 5
USP19 increases the amount of MYH.
| 3
USP19 increases the amount of MYH. 3 / 3
| 3

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"USP19 depletion also increased MHC and tropomyosin mRNA levels, suggesting that this effect is due to increased transcription."

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"Mutant forms of USP19 lacking deubiquitinating activity increased MHC and tropomyosin levels."

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"USP19 depletion also increased MHC and tropomyosin mRNA levels, suggesting that this effect is due to increased transcription."
USP19 activates MYH.
| 2
USP19 activates MYH. 2 / 2
| 2

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"USP19 siRNA reversed the suppression of MHC by these agents."

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"Depletion of USP19 reversed the dexamethasone suppression of MHC."
USP19 affects HTT
| 5
USP19 activates HTT. 5 / 5
| 5

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"The major finding of this work is that cytoplasmic USP19 can promote aggregation of the polyQ expanded Atx3 and Htt proteins by up-regulating their protein levels."

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"Based on these lines of evidence, we propose a model for triage decision of the Htt protein modulated by USP19 through HSP90."

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"A mechanism of auto-inhibition of USP19 and activation by HSP90 is proposed, on which USP19 modulates the protein level of polyQ expanded huntingtin in cells."

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"Cytoplasmic Ubiquitin Specific Protease 19 (USP19) Modulates Aggregation of Polyglutamine Expanded Ataxin-3 and Huntingtin through the HSP90 Chaperone."

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"Our previous study has demonstrated that cytoplasmic ubiquitin specific protease 19 (USP19), through interacting with HSP90, up-regulates the protein levels of the N-terminal 552-residue fragments of Htt (Htt-N552) with normal and expanded polyQ, and consequently increases the aggregates formed by polyQ expanded Htt-N552 31."
USP19 affects FOXO1
| 5
USP19 deubiquitinates FOXO1.
| 4
USP19 deubiquitinates FOXO1. 4 / 4
| 4

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"In microglia, FOXO1 positively regulated IL-10 expression at the transcriptional level, and USP19 was implicated in the deubiquitination of FOXO1."

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"USP19 mediated the deubiquitination of FOXO1 to activate IL-10/IL-10R1 signaling, thereby regulating microglial M1/M2 polarization."

reach
"Bioinformatic analysis based on Ubibrowser 2.0 predicted that USP19 might mediate the deubiquitination of FOXO1."

reach
"Low-dose lipopolysaccharide (LPS) activates the IL-10/IL-10R1 signaling pathway through the deubiquitination of FOXO1 mediated by USP19, regulates microglial polarization, reduces brain edema, blood-brain barrier disruption, neuroinflammation, etc., and has a protective effect on EBI [88]."
USP19 ubiquitinates FOXO1.
| 1
USP19 leads to the ubiquitination of FOXO1. 1 / 1
| 1

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"co-IP revealed that lack of USP19 increased the ubiquitination of FOXO1 in SH-SY5Y cells (Fig. 5B)."
USP19 affects FKBP6
5 |
5 |

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reach
"Our study reveals that USP19 elevates the intracellular Ca 2+ concentration to alter ER stress via regulation of BAG6 and BCL2 stability and may be a viable therapeutic target for TNBC therapy."

reach
"Histological and immunohistochemical (IHC) analyses found that USP19 enhanced the expression of ER stress‐related proteins to induce the ER stress response (Figure 7E)."

reach
"Taken together, these results indicated that USP19 can induce the activation of ER stress‐related apoptosis through stabilization of BAG6 expression levels, thereby ameliorating TNBC progression in vivo.3.8 METTL14 reduced m 6 A RNA methylation of USP19 ."

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"Knockdown of BAG6 in mice also reversed ER stress triggered by USP19, indicating that the essential effects of USP19 on ER stress depend on BAG6 stability."

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"Collectively, USP19 can significantly increase the unfolded protein entering the ER and induce ER stress, by reducing BAG6 ubiquitination to kill TNBC cells.m A methylation had received a lot of attention recently and was now widely acknowledged as the most frequent and prolific alteration on mRNA and as a mechanism to control RNA translation, stability, and degradation."
USP19 affects DERL1
1 | 1 3
1 | 1 3

sparser
"Intriguingly, although overexpressed USP19 interacts with Derlin-1 and other ERAD machinery factors in the membrane, endogenous USP19 is mostly in the cytosol where it binds Hsp90."

sparser
"It was shown that USP19 can interact with Derlin-1 and overexpression of wild type USP19 can inhibit the degradation of a few model ERAD substrates."

sparser
"Accordingly, we detect neither interaction of endogenous USP19 with Derlin-1 nor significant effect on ERAD by USP19 depletion."

reach
"It was shown that USP19 can interact with Derlin-1 and overexpression of wild type USP19 can inhibit the degradation of a few model ERAD substrates."

No evidence text available
USP19 affects CFTR
1 | 4
USP19 activates CFTR.
| 4
USP19 activates CFTR. 4 / 4
| 4

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"USP19 was reported to rescue the CFTR Delta508 mutant, which is an ERAD substrate."

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"USP19 rescues the ERAD substrates cystic fibrosis transmembrane conductance regulator (CFTR) DeltaF508 and T-cell receptor-alpha (TCRalpha) from proteasomal degradation."

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"Interestingly, USP19 is a target gene of the unfolded protein response (UPR) and was found to rescue CFTR DeltaF508 from ERAD."

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"In preceding studies the ER localised USP19 was shown to rescue the ERAD substrates cystic fibrosis transmembrane conductance regulator (CFTR) DeltaF508 and T-cell receptor-alpha (TCRalpha) from proteasomal degradation 109."
USP19 binds CFTR.
1 |
1 |

No evidence text available
USP19 affects ATXN3
| 5
USP19 activates ATXN3. 5 / 5
| 5

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"We next examined the effect of usp19 silencing on the protein levels of Atx3 100Q and Htt-N552 100Q, and observed that knockdown of USP19 significantly reduced the protein levels of Atx3 100Q (XREF_FIG) and Htt-N552 100Q (XREF_FIG) both in HEK 293T cells and in human retinal pigment epithelial (RPE1) cells (XREF_SUPPLEMENTARY)."

reach
"The major finding of this work is that cytoplasmic USP19 can promote aggregation of the polyQ expanded Atx3 and Htt proteins by up-regulating their protein levels."

reach
"The regulatory function of USP19 was recently confirmed in a study demonstrating that USP19 interacts directly with chaperone Hsp90 and upregulates the aggregation of poly-Q containing the proteins Ataxin-3 and Huntingtin, which causes spinocerebellar ataxia type-3 and Huntington’s disease, respectively [90]."

reach
"Cytoplasmic Ubiquitin Specific Protease 19 (USP19) Modulates Aggregation of Polyglutamine Expanded Ataxin-3 and Huntingtin through the HSP90 Chaperone."

reach
"Beclin-1 stabilization is also promoted by USP19 and ataxin 3, which specifically removes K11- and K48-ubiquitin chain from Belcin-1, respectively [38, 39]."
USP19 affects AIP
2 | 1 2
2 | 1

reach
"AIP interactions with Usp19, DNAAF2, NADSYN1, p23, Hsp105, and BclG are shown in Fig. 4C and FKBP51 interactions with these proteins were found to be similar to those of AIP (not shown)."

No evidence text available

No evidence text available
AIP binds FKBP51 and USP19. 1 / 1
| 1

sparser
"We confirmed interaction of ubiquitin carboxypeptidase Usp19 with two dcTPR proteins, AIP and FKBP51, via the dicarboxylate clamp mechanism."
| 1

sparser
"We obtained moderate to strong labeling for Usp19, DNAAF2, and NADSYN1 interactions with AIP and surprisingly strong labeling of AIP–p23 interactions."
USP19 affects ACSL4
| 5
| 5

sparser
"This hypothesis was supported by co‐immunoprecipitation assays, which demonstrated that wild‐type ZDHHC2 overexpression significantly reduced the ACSL4USP19 protein–protein interaction (Figure  xref ; Figure  xref , Supporting Information ), but did not influence the binding between ACSL4 and the USP19 C152S mutant, which cannot be palmitoylated by ZDHHC2 (Figure  xref )."

sparser
"Meanwhile, compared to enzalutamide‐sensitive cell lines, the ACSL4USP19 protein–protein interaction was weakened in resistant cell lines, which matches our hypothesis (Figure  xref , Supporting Information)."

sparser
"PLA was performed to analyze the interaction between USP19 and ACSL4 after ZDHHC2 knockdown."

sparser
"Coimmunoprecipitation was performed using three CRPC cell lines to demonstrate the endogenous interaction between USP19 and ACSL4 (Figure  xref , Supporting Information)."

sparser
"ZDHHC2 knockdown significantly enhanced the interaction between USP19 and ACSL4 (Figure  xref )."
Non-structural protein 5A affects USP19
5 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
MAP3K7 affects USP19
2 | 1 2
2 | 1 2

No evidence text available

sparser
"Recently, our group found that USP19 is associated with TAK1 in a TNFα- or IL-1β-dependent manner and specifically deconjugates K63- and K27-linked polyubiquitin chains from TAK1, leading to the impairment of TAK1 activity and the disruption of TAK1-TAB2/3 complex."

reach
"The USP19/TAK1 interaction and ubiquitinated TAK1 levels were assayed by coimmunoprecipitation (Co-IP) assays and IB."

sparser
"Mechanistically, USP19 interacted with TAK1 in a TNF-α- or IL-1β-dependent manner and specifically deconjugated K63- and K27-linked polyubiquitin chains from TAK1, leading to the impairment of TAK1 activity and the disruption of the TAK1-TAB2/3 complex."

No evidence text available
FKBP6 affects USP19
5 |
5 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
EWSR1 affects FLI1, and USP19
| 5
USP19 binds FLI1 and EWSR1. 5 / 5
| 5

sparser
"USP19 interacts with the N-terminal domain of EWS-FLI1 and full length EWSR1."

sparser
"Most interestingly, co-expression of active USP19 with EWS-FLI1 stabilized the fusion protein whereas full length EWSR1 and FLI1 protein levels remained unchanged even though binding of USP19 to both EWS-FLI1 and full length EWSR1 was observed in immunoprecipitation experiments."

sparser
"More recently, USP19 was shown to deubiquitinate and stabilise EWS-FLI-1 fusions but not FLI-1, likely because USP19 bound to EWS-FLI-1 within N-terminal EWS sequences [ xref ]."

sparser
"It is therefore likely that EWS-FLI1 interacts with USP19 in the cytosol as the fusion protein displays a constant turnover and is shuffling in and out of the nucleus xref ."

sparser
"For this, we co-expressed myc-USP19 with 3xflag-EWS-FLI1 wild-type or mutants (K298R or K380R) and observed binding of USP19 to both EWS-FLI1 mutants, indicating that the interaction itself is independent of the presence of an ubiquitin chain (Fig.  xref )."
DERL1 affects USP19
1 | 1 3
1 | 1 3

sparser
"Intriguingly, although overexpressed USP19 interacts with Derlin-1 and other ERAD machinery factors in the membrane, endogenous USP19 is mostly in the cytosol where it binds Hsp90."

sparser
"It was shown that USP19 can interact with Derlin-1 and overexpression of wild type USP19 can inhibit the degradation of a few model ERAD substrates."

sparser
"Accordingly, we detect neither interaction of endogenous USP19 with Derlin-1 nor significant effect on ERAD by USP19 depletion."

reach
"It was shown that USP19 can interact with Derlin-1 and overexpression of wild type USP19 can inhibit the degradation of a few model ERAD substrates."

No evidence text available
AIP affects USP19
2 | 1 2
2 | 1

reach
"AIP interactions with Usp19, DNAAF2, NADSYN1, p23, Hsp105, and BclG are shown in Fig. 4C and FKBP51 interactions with these proteins were found to be similar to those of AIP (not shown)."

No evidence text available

No evidence text available
AIP binds FKBP51 and USP19. 1 / 1
| 1

sparser
"We confirmed interaction of ubiquitin carboxypeptidase Usp19 with two dcTPR proteins, AIP and FKBP51, via the dicarboxylate clamp mechanism."
| 1

sparser
"We obtained moderate to strong labeling for Usp19, DNAAF2, and NADSYN1 interactions with AIP and surprisingly strong labeling of AIP–p23 interactions."
ACSL4 affects USP19
| 5
| 5

sparser
"This hypothesis was supported by co‐immunoprecipitation assays, which demonstrated that wild‐type ZDHHC2 overexpression significantly reduced the ACSL4USP19 protein–protein interaction (Figure  xref ; Figure  xref , Supporting Information ), but did not influence the binding between ACSL4 and the USP19 C152S mutant, which cannot be palmitoylated by ZDHHC2 (Figure  xref )."

sparser
"Meanwhile, compared to enzalutamide‐sensitive cell lines, the ACSL4USP19 protein–protein interaction was weakened in resistant cell lines, which matches our hypothesis (Figure  xref , Supporting Information)."

sparser
"PLA was performed to analyze the interaction between USP19 and ACSL4 after ZDHHC2 knockdown."

sparser
"Coimmunoprecipitation was performed using three CRPC cell lines to demonstrate the endogenous interaction between USP19 and ACSL4 (Figure  xref , Supporting Information)."

sparser
"ZDHHC2 knockdown significantly enhanced the interaction between USP19 and ACSL4 (Figure  xref )."
Ozone affects USP19
4 |
Ozone increases the amount of USP19.
3 |
Ozone increases the amount of USP19. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
Ozone decreases the amount of USP19.
1 |
Ozone decreases the amount of USP19. 1 / 1
1 |

No evidence text available
Estrogen affects USP19
1 | 3
Estrogen decreases the amount of USP19.
1 | 2
Estrogen decreases the amount of USP19. 3 / 3
1 | 2

No evidence text available

reach
"In support of a role for USP19 in myogenesis, estrogen acting via the ERα receptor can suppress differentiation and increase expression of USP19 in cultured muscle cells ( Ogawa et al., 2011 )."

reach
"In support of this, estrogen acting via the ERalpha receptor can suppress differentiation and increase expression of USP19 in cultured muscle cells (Ogawa et al., 2011)."
Estrogen increases the amount of USP19.
| 1
Estrogen increases the amount of USP19. 1 / 1
| 1

reach
"Collectively, the work by Ogawa and co-workers provides evidence that estrogen increases the expression of USP19 and contributes to muscle atrophy."
Bisphenol A affects USP19
4 |
Bisphenol A increases the amount of USP19.
2 |
Bisphenol A increases the amount of USP19. 2 / 2
2 |

No evidence text available

No evidence text available
Bisphenol A demethylates USP19.
2 |
Bisphenol A demethylates USP19. 2 / 2
2 |

No evidence text available

No evidence text available
YAP1 affects USP19
| 2 2
| 2 2

reach
"Besides, the nuclear and cytoplasmic separation CoIP assay indicated that the interaction between USP19 and YAP occurred in the cytoplasm ( Fig. 3 C)."

sparser
"Moreover, the data from TCGA database displayed that USP19 was positively associated with YAP1 and its target genes ( CTGF and CYR61 ) ( Fig. 1 J, Fig. S2E and F )."

reach
"To further elucidated the cooperation between USP19 and YAP, we constructed the deletion construction of them ( Fig. 2 D)."

sparser
"Besides, the nuclear and cytoplasmic separation CoIP assay indicated that the interaction between USP19 and YAP occurred in the cytoplasm ( Fig. 3 C)."
USP19 affects tropomyosin
| 4
USP19 increases the amount of tropomyosin. 4 / 4
| 4

reach
"Depletion of all isoforms of USP19 increased myogenin (XREF_FIG) and tropomyosin and MHC protein levels (XREF_FIG), as previously reported, and enhanced myotube formation and fusion (XREF_FIG)."

reach
"USP19 depletion also increased MHC and tropomyosin mRNA levels, suggesting that this effect is due to increased transcription."

reach
"USP19 depletion also increased MHC and tropomyosin mRNA levels, suggesting that this effect is due to increased transcription."

reach
"Mutant forms of USP19 lacking deubiquitinating activity increased MHC and tropomyosin levels."
USP19 affects proteolysis
| 4
| 4

reach
"Conclusions : Loss of USP19 suppresses proteolysis upon glucocorticoid stimulation, but enhances protein synthesis upon fasting."
| PMC

reach
"We found that ectopic expression of USP19 increased PAH protein level, whereas depletion of USP19 promoted PAH protein degradation."

reach
"USP19 suppresses protein synthesis and enhances protein degradation (ubiquitin proteasome system and autophagy) suggesting that it acts on an upstream signalling pathway (s)."

reach
"The deubiquitinating activity of USP19 prevents protein degradation and increases the abundance of both PAH protein variants."
| 4
| 3

reach
"A subsequent study showed that both CSPα and HSC70 functioned downstream of USP19 to promote MAPS as knockdown of CSPα or HSC70 inhibited USP19-induced protein secretion (Xu et al., 2018)."

reach
"This likely represents a small fraction of physiological MAPS cargoes because the experiments were conducted under serum-depleted culture condition, which, while being necessary to eliminate serum contaminants from the secretome and allow mass spectrometry, is known to diminish USP19-mediated unconventional protein secretion (21)."

reach
"Both CSPα and HSC70 function downstream of USP19 because the downregulation of CSPα or HSC70 inhibits USP19-induced protein secretion (Xu et al., 2018)."
| 1

reach
"Instead, we found that USP19 overexpression promoted the release of certain cytosolic proteins while its inactivation inhibited unconventional protein secretion in mammalian cells."
USP19 affects pathogenic potential Th17 cells
| 4
USP19 inhibits pathogenic potential Th17 cells. 4 / 4
| 4

eidos
"Collectively , our findings indicate that USP19 selectively suppresses the pathogenic potential of Th17 cells and offer novel strategies for treating autoimmune diseases ."

eidos
"Collectively , our findings indicate that the USP19 selectively suppresses the pathogenic potential of Th17 cells and suggest strategies for treating autoimmune diseases ."

eidos
"Collectively , our findings indicate that the USP19 selectively suppresses the pathogenic potential of Th17 cells , and provides novel strategies for treating autoimmune diseases ."

eidos
"In line with our in vitro findings , these in vivo observations indicate that USP19 suppresses pathogenic potential of Th17 cells , and therefore ameliorates autoimmunity ."

reach
"In similar assays, USP19 also inhibited LPS-induced signaling in 293-TLR4 cells (Supplementary Fig. 1b)."

reach
"Our study reported that lack of USP19 reversed the protective effect of low-dose LPS on inflammation in SAH mice, as well as other neurological parameters."

reach
"USP19 deficiency increases the production of type I interferons (IFN) and proinflammatory cytokines induced by poly(I:C) or LPS in vitro and in vivo."

reach
"Deficiency of USP19 increased serum cytokine levels after administration of poly(I:C) and LPS, and promoted inflammatory death caused by administration of poly(I:C) or LPS, or infection of Salmonella typhimurium."
| 2

reach
"Here we found that USP19 overexpression significantly promotes the secretion of soluble and aggregated misfolded TDP-43, requiring both ER anchoring and ubiquitin peptidase activity."

reach
"Notably, our recent work further unraveled that the deubiquitinating enzyme USP19 enables activation of its co-localized NFE2L1 in proximity to the ER and, in turn, NFE2L1 in close cooperation with NFE2L2/Nrf2 can tightly govern the transcriptional expression of USP19 and other DUBs insomuch as to form a positive feedback regulatory circuit [49]."

reach
"Under these conditions, cell viability was unaffected and ER stress was not induced by USP19 knockdown."
USP19 deubiquitinates endoplasmic reticulum.
| 1
| 1

reach
"In contrast, our studies have clearly demonstrated that USP19 catalytic activity is essential for its effects on differentiation and argue that USP19 is deubiquitinating an ER localized protein that represses the ER stress response."
USP19 affects cell growth
| 4
| 4

reach
"Results indicated that ectopic USP19 expression significantly promoted CRC cell growth through elevating ME1 protein levels ( Figures S3 A and S3B)."

reach
"The results showed that knockdown of USP19 suppressed cell proliferation in SGC7901 cells while overexpressing USP19 accelerated cell growth in GES1 cells, as determined by MTT assay (P < 0.01, XREF_FIG and XREF_FIG)."

reach
"18 Genetic depletion of USP19 reduced Ewing sarcoma cell growth in vitro and in mice largely through destabilizing EWS–FLI1 proteins."

reach
"Therefore, the USP19 modulation of prostate cancer cell growth occurs through a regulation of cell cycle progression from G0/G1 to S phase and does not affect the rate of apoptotic cell death."
USP19 affects Ubiquitin
| 3 1
USP19 inhibits Ubiquitin.
| 2
| 2

reach
"Taken together, USP19 suppresses pTh17 differentiation and pathogenic potential through the removal of K63-linked ubiquitin chain from lysine 313 of RORγt, which is required for recruiting coactivator SRC3 and essential for the expression of proinflammatory effectors (Fig. 6E)."

reach
"RNF216 interacts with and ubiquitinates Beclin 1 at lysine 48, thereby contributing to Beclin 1 degradation, while SLC9A3R1 and USP19 blocks ubiquitin dependent Beclin 1 degradation by interacting with Beclin 1 and removing the K11 linked ubiquitin respectively."
USP19 binds Ubiquitin.
| 1
| 1

sparser
"As the main ubiquitin acceptor site for EWS-FLI1 degradation is located in the FLI1 terminus xref , we were next interested in identifying whether USP19 still interacts with EWS-FLI1 ubiquitin acceptor site mutants."
USP19 activates Ubiquitin.
| 1
USP19 bound to MGMT activates Ubiquitin. 1 / 1
| 1

reach
"Mechanistically, USP19 binds to MGMT and then deubiquitinates MGMT, causing the removal of ubiquitin chains, which stabilize MGMT."
USP19 affects SNCA
1 | 3
USP19 activates SNCA.
| 3
USP19 activates SNCA. 3 / 3
| 3

reach
"Although USP19 stimulates alpha-synuclein secretion, exosome dependent tau secretion is not enhanced.113 4 Alternative Spreading Mechanisms."

reach
"However, under this condition, wild type (WT) USP19 is able to promote the secretion of misfolded alpha-Synuclein (alpha-Syn) in a dose dependent manner, as demonstrated previously 3."

reach
"Recently, the DUB, USP19, was shown to promote misfolded alpha-synuclein secretion [XREF_BIBR]."
USP19 increases the amount of SNCA.
1 |
USP19 increases the amount of SNCA. 1 / 1
1 |

"USP19 promoted the secretion of a-syn, suggesting that MAPS is an unconventional secretion pathway utilized by a-syn, particularly under conditions of proteasomal impairment, which has been repeatedly linked to Parkinson’s disease. "
USP19 affects RORC
1 | 3
1 | 3

sparser
"To test this possibility, we first examined whether USP19 would bind with RORγt under pTh17 polarizing conditions."

sparser
"The interaction between USP19 and RORγt in pTh17 cells was validated by co-immunoprecipitation (co-IP) ( xref )."

No evidence text available

sparser
"Although our results clearly demonstrate that USP19 interacts directly with RORγt and constrains pathogenic Th17 cell programming and response amplification, deubiquitinases also have effects on multiple other substrates."
USP19 affects RAR
| 2 2
USP19 inhibits RAR.
| 2
USP19 inhibits RAR. 2 / 2
| 2

reach
"RAR target genes were decreased by the overexpression of CORO2A, and increased by the knockdown of USP19 (XREF_SUPPLEMENTARY)."

reach
"USP19 and CORO2A mediate the transcriptional repression of RAR."
USP19 binds RAR.
| 2
USP19 binds CORO2A and RAR. 2 / 2
| 2

sparser
"The results suggest that USP19 may be associated with the transcriptional regulation of RAR via CORO2A as one of the components for the NCoR complex during the adipogenesis."

sparser
"These results indicate that USP19 is associated with the regulation of transcriptional repression of RAR through CORO2A."
USP19 affects MAP1LC3
| 1 3
USP19 decreases the amount of MAP1LC3.
| 3
USP19 decreases the amount of MAP1LC3. 3 / 3
| 3

reach
"USP19 knockdown in CSE-treated BEAS-2B cells increased cell survival, OCR, and protein levels of LC3 II/I, reduced TOM20 protein level, and mitigated the reduction in mitochondrial membrane potential."

reach
"Moreover, the increased protein levels of LC3 II/I and cell survival induced by USP19 knockdown were alleviated by FUNDC1 overexpression in CSE-treated BEAS-2B cells."

reach
"Western blotting and immunofluorescence experiments of proteins related to autophagy showed that USP19 overexpression substantially reduced the protein expression of P62 in LoVo and SW480 cells while significantly elevating the protein expression of Beclin1 and LC3, as compared to the L-OHP group (Fig. 4B–D)."
USP19 activates MAP1LC3.
| 1
USP19 activates MAP1LC3. 1 / 1
| 1

eidos
"In CSE-treated BEAS-2B cells , USP19 knockdown reduced FUNDC1 and LC3 II / I , increased the levels of TOM20 , improved cell survival , mitochondrial membrane potential , and OCR , and inhibited apoptosis ."
USP19 affects FlaG
| 4
| 4

sparser
"As expected, USP19 was the most abundant protein in the USP19-FLAG immunoprecipitates and MXRA8 in the controls ( xref B )."

sparser
"Then, immunoprecipitates were applied to unbiased high-resolution mass spectrometry, and the relative abundance of proteins co-immunoprecipitated with USP19-FLAG was compared to that of proteins co-immunoprecipitated with the FLAG-tagged control protein."

sparser
"We therefore performed biochemical fractionation using cells expressing GFP and GFP1–10 either alone or together with FLAGUSP19."

sparser
"To further investigate whether USP19 directly deubiquitinated MGMT, we performed an in vitro deubiquitination assay using 293T cell expressed FLAGUSP19, S‐MGMT independently and purified by FLAG‐tag, S‐tag affinity agrose beads, respectively."
USP19 affects DNM1L
2 | 1 1
USP19 binds DNM1L.
2 | 1
2 | 1

No evidence text available

No evidence text available

sparser
"Interestingly, Chai et al. also found that USP19 could interact with DRP1, although the exact mechanism is still unknown, and whether USP19 promotes the oligomeric transfer of DRP1 to MERCSs requires further investigation ( xref )."
USP19 activates DNM1L.
| 1
USP19 bound to FUNDC1 activates DNM1L. 1 / 1
| 1

reach
"Simultaneously, the deubiquitinating enzyme ubiquitin specific peptidase 19 (USP19), which is located in the endoplasmic reticulum, accumulates at the MAM and binds to the mitochondrial outer membrane protein FUNDC1, inducing its deubiquitination, promoting the oligomerization of dynamin-related protein 1 (DRP1), and resulting in mitochondrial fission (Zhang et al., 2022)."
USP19 affects DNAJC5
| 3 1
USP19 binds DNAJC5.
| 1 1
| 1 1

reach
"Because HSC70 forms a stable complex with its co-chaperone DNAJC5, which was recently implicated in unconventional secretion of Tau and alpha-Syn 15, we also tested whether USP19 could interact with DNAJC5."

sparser
"Because HSC70 forms a stable complex with its co-chaperone DNAJC5, which was recently implicated in unconventional secretion of Tau and α-Syn xref , we also tested whether USP19 could interact with DNAJC5."
USP19 inhibits DNAJC5.
| 1
USP19 inhibits DNAJC5. 1 / 1
| 1

reach
"If so, depletion of USP19 should abolish DNAJC5 stimulated secretion."
USP19 activates DNAJC5.
| 1
USP19-C506S activates DNAJC5. 1 / 1
| 1

reach
"Moreover, we found that WT USP19 but not USP19 C506S also enhanced DNAJC5 secretion, consistent with the idea that when USP19 induced MAPS, DNAJC5 accompanied substrates to the cell exterior."
RORC affects USP19
1 | 3
1 | 3

sparser
"To test this possibility, we first examined whether USP19 would bind with RORγt under pTh17 polarizing conditions."

sparser
"The interaction between USP19 and RORγt in pTh17 cells was validated by co-immunoprecipitation (co-IP) ( xref )."

No evidence text available

sparser
"Although our results clearly demonstrate that USP19 interacts directly with RORγt and constrains pathogenic Th17 cell programming and response amplification, deubiquitinases also have effects on multiple other substrates."
Mice affects USP19
| 4
Mice increases the amount of USP19.
| 2
Mice increases the amount of USP19. 2 / 2
| 2

reach
"In vivo experiments in mice suggested that TIPE3 inhibited apoptosis of tumor cells and promoted macrophage M2-type polarization in tumor tissues by up-regulating the protein expression of USP19."

reach
"Ogawa et al. report that E2 treatment of ovx mice decreased the ratio of skeletal muscle mass to body weight and increased ubiquitin-specific peptidase 19 expression, indicating that E2 inhibits myogenesis in vivo."
Mice activates USP19.
| 2
Mice activates USP19. 2 / 2
| 2

reach
"Although the majority of our recent work has been focused on TNBC cell lines, USP19 overexpression mice have allowed us to more thoroughly study and confirm the specific functional role of USP19."

reach
"These investigators observed that silencing USP19 expression increases soleus muscle mass in females, but not in males and that estrogen administration to male mice increased USP19 and decreased soleu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
MARCHF6 affects USP19
| 2 1
| 2 1

reach
"Since transmembrane USP19 and MARCH6 have been shown to be localized to the ER, we first assessed the possibility that USP19 interacts with MARCH6."

sparser
"Since transmembrane USP19 and MARCH6 have been shown to be localized to the ER, we first assessed the possibility that USP19 interacts with MARCH6."

reach
"We found that USP19 also interacts with and stabilizes MARCH6."
Hypoxia affects USP19
| 4
Hypoxia decreases the amount of USP19.
| 3
Hypoxia decreases the amount of USP19. 3 / 3
| 3

reach
"Hence, pioneering researches rarely focused on whether and how hypoxia regulates the Beclin-1 expression in cervical cancer.In this study, we found that hypoxia will induce significant decrease of bot[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Our findings revealed that the hypoxia treatment could obviously decrease the USP19 expression ( Fig. 2 A and B )."

reach
"Hypoxia not only diminishes Beclin-1 and USP19 levels but also triggers a dual suppression mechanism through a coherent feed-forward loop."
Hypoxia activates USP19.
| 1
Hypoxia activates USP19. 1 / 1
| 1

reach
"Subsequently, the active hypoxia-induced signal (aHs) significantly downregulated the production of both USP19 and Beclin-1 (reactions 3 and 5 in Table S1 )."
FlaG affects USP19
| 4
| 4

sparser
"As expected, USP19 was the most abundant protein in the USP19-FLAG immunoprecipitates and MXRA8 in the controls ( xref B )."

sparser
"Then, immunoprecipitates were applied to unbiased high-resolution mass spectrometry, and the relative abundance of proteins co-immunoprecipitated with USP19-FLAG was compared to that of proteins co-immunoprecipitated with the FLAG-tagged control protein."

sparser
"We therefore performed biochemical fractionation using cells expressing GFP and GFP1–10 either alone or together with FLAGUSP19."

sparser
"To further investigate whether USP19 directly deubiquitinated MGMT, we performed an in vitro deubiquitination assay using 293T cell expressed FLAGUSP19, S‐MGMT independently and purified by FLAG‐tag, S‐tag affinity agrose beads, respectively."
BIRC2 affects USP19
1 | 1 1
1 | 1 1

reach
"Here we report that USP19, a deubiquitinating enzyme, interacts with cellular IAP 1 (c-IAP1) and c-IAP2."

No evidence text available

sparser
"As shown by Western Blot analysis ( xref and xref ) and epifluorescence microscopy ( xref and xref ; xref ), in contrast to control cells, USP19 expression was markedly decreased in cardiac myocytes treated with DOX, resulting in reduced protein complexes between USP19 and c-IAP1."
Resveratrol affects USP19
| 3
Resveratrol inhibits USP19.
| 1
| 1

reach
"In a myocardial I/R model of diabetic rats, I/R activates ubiquitin-specific protease 19 (USP19) and Beclin1, leading to ferroptosis, while resveratrol can inhibit iron death by downregulating USP19/Beclin1 and upregulating GPX4 and FTH1, thus reducing myocardial cell I/R injury."
Resveratrol decreases the amount of USP19.
| 1
Resveratrol decreases the amount of USP19. 1 / 1
| 1

reach
"Resveratrol intervention downregulated the expression of USP19 and Becklin-1, but upregulated the expression of GPX4 and FTH1 significantly (All P < 0.05) ( Fig. 5 E )."
Resveratrol activates USP19.
| 1
| 1

reach
"Resveratrol also modulates the ubiquitin-specific peptidase 19 (USP19)/Beclin1-mediated autophagy pathway, which is implicated in the regulation of ferroptosis [83,84]."
| 3
| 3

reach
"Dexamethasone treatment lowered MHC and increased USP19."

reach
"It is also possible that the deubiquitinating enzyme USP19, which is induced in atrophying skeletal muscle and stimulated by dexamethasone played a role in mediating the negative balance between muscle protein synthesis and proteolysis."

reach
"Dexamethasone or TNF-alpha treatment lowered MHC and increased USP19."

reach
"Ogawa et al. report that E2 treatment of ovx mice decreased the ratio of skeletal muscle mass to body weight and increased ubiquitin-specific peptidase 19 expression, indicating that E2 inhibits myogenesis in vivo."

reach
"Interestingly, knockdown of USP19, which is reported to suppress myogenesis [27], did not positively affect the fusion index in this experimental setup (Fig. 1C)."

reach
"The ability of USP19 to modulate protein synthesis, degradation as well as myogenesis suggest that it is modulating a signaling pathway(s) that is upstream of these processes."
USP19 affects pTh17
| 3
USP19 inhibits pTh17. 3 / 3
| 3

reach
"Consistent with this finding, the deubiquitinase USP19 was shown to suppress pTh17 differentiation in vitro and Th17-mediated pathogenesis in vivo."

reach
"Consistent with these findings, the deubiquitinase USP19 suppressed pTh17 differentiation in vitro as well as Th17-mediated pathogenesis in vivo."

reach
"Consistent with these findings, a deubiquitinase USP19 suppressed pTh17 differentiation in vitro as well as Th17-mediated pathogenesis in murine autoimmune models."
USP19 affects lysosomal proteins
| 3
USP19 increases the amount of lysosomal proteins.
| 2
USP19 increases the amount of lysosomal proteins. 2 / 2
| 2

reach
"Loss of USP19 impairs release of lysosomal proteins, including legumain (LGMN) and a number of cathepsins."

reach
"Intriguingly, loss of USP19 most significantly diminished levels of lysosomal proteins in the conditioned media, without significantly altering their levels in the lysate."
USP19 decreases the amount of lysosomal proteins.
| 1
USP19 decreases the amount of lysosomal proteins. 1 / 1
| 1

reach
"Thus, we continued investigating its secretion to dissect the mechanism by which USP19 ablation impaired the release of lysosomal proteins."

reach
"USP19 inhibits inflammation and promotes M2-like macrophage polarization by regulating NLRP3 function through autophagy (15)."

eidos
"USP19 suppresses inflammation and promotes M2-like macrophage polarization by manipulating NLRP3 function via autophagy ."

reach
"USP19 suppresses inflammation and promotes M2 like macrophage polarization by manipulating NLRP3 function via autophagy."

reach
"Moreover, USP19-ER inhibits TGF-beta-induced epithelial-mesenchymal transition (EMT), whereas USP19-CY enhances EMT, as well as the migration and extravasation of breast cancer cells."

reach
"These results suggest that USP19-CY promotes TGF-β-induced EMT, as well as basal and TGF-β-mediated cell migration and invasion."

reach
"In contrast, USP19-CY promotes TGF-β/SMAD-induced breast and lung cancer cell EMT, cell migration and extravasation in vitro and in vivo."

reach
"In addition, ACAT1 is a target for proteasomal degradation as USP19 was found to enhance cholesterol esterification through deubiquitinating and stabilizing ACAT1 in hepatocellular carcinoma cells [ 3[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Loss of either SOAT1 or USP19 dramatically attenuates cholesterol esterification and hepatocarcinogenesis in the p53-deficient mice fed with either normal chow diet (NCD) or high-cholesterol, high-fat diet (HCHFD)."

reach
"Also, worth investigating is whether USP19 inhibitors are promising drugs against tumors in general, or only some specific types of tumors, like liver cancers with p53 deletions/mutations that originate in a NAFLD background.In conclusion, this study shows that p53 deletion/mutation increases USP19, which in turn stabilizes SOAT1, increases cholesterol esterification and accelerates tumor progression (Figure 1)."
USP19 affects ZDHHC2
| 3
| 3

sparser
"Next, the binding region of USP19 to ZDHHC2 was analyzed via molecular docking (Figure  xref ) and GST pull‐down assays (Figure  xref ), and we found that USP19 binds to the C‐terminal region of ZDHHC2."

sparser
"Coimmunoprecipitation confirmed the endogenous interaction between ZDHHC2 and USP19 (Figure  xref ), GST pull‐down verified the direct physical interaction (Figure  xref ), and PLA further demonstrated spatial proximity interactions (Figure  xref )."

sparser
"Given that ZDHHC2 regulates ACSL4 through USP19, we subsequently investigated the functional impact of the ZDHHC2USP19 axis on ferroptosis."
USP19 affects TRAF2
| 3
USP19 ubiquitinates TRAF2.
| 1
USP19 ubiquitinates TRAF2. 1 / 1
| 1

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"Interestingly, USP19 which constitutively de-ubiquitinylates and disables c-IAP-1 from ubiquitinating and targeting TRAF2 for proteasomal degradation was also reduced in cardiac myocytes treated with doxorubicin - a finding concordant with impaired NF-κB activation."
USP19 inhibits TRAF2.
| 1
USP19 inhibits TRAF2. 1 / 1
| 1

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"As shown in (Figure 5D and 5E), in contrast to vector alone, exogenous USP19 de-ubiquitinated c-IAP1 and suppressed DOX- induced degradation of TRAF2 (Figure 5F and 5G)."
USP19 deubiquitinates TRAF2.
| 1
USP19 deubiquitinates TRAF2. 1 / 1
| 1

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"For example, myocardial-specific expression of A20, a DUB, improves left ventricular function and alleviates compensatory myocardial hypertrophy induced by myocardial infarction.7 Another 2 DUBs, USP4 and USP18, attenuate hypertension-induced pathological cardiac hypertrophy by directly binding to TAK1.8 9 In addition, the deubiquitinase OTUD1 regulates the progression of diabetic cardiomyopathy by regulating YB-1 and inhibiting its activation10 and USP19 alleviates doxorubicin-induced cardiomyopathy by deubiquitinating TRAF2 and preventing its degradation."
USP19 affects TNF
| 1 2
USP19 inhibits TNF. 3 / 3
| 1 2

sparser
"For example, USP19 inhibits Tnf and Il1b-triggered NF-κB activation by targeting TAK1 uncoupling of k27 and k63-linked polyubiquitin chains ( xref )."

reach
"USP19 attenuates the TNF-α– and IL-1β–triggered inflammatory response via deubiquitination of TGF-β-activated kinase 1."

sparser
"Removal of K27- and K63-linked chains by USP19 inhibited TNFα- and IL-1β-induced NFκB activation, suggesting that these ubiquitin chains normally activate TAK1 downstream signaling ( xref )."
USP19 affects TLR3/4
| 3
USP19 inhibits TLR3/4. 3 / 3
| 3

reach
"These data suggest that USP19 attenuates TLR3/4-mediated inflammatory responses in mice."

reach
"USP19 negatively regulates TLR3/4-mediated signaling."

reach
"Collectively, these results suggest that USP19 negatively regulates TLR3/4-mediated induction of downstream effector genes in primary mouse immune cells or fibroblasts."
USP19 affects TGFB
| 3
USP19 activates TGFB. 3 / 3
| 3

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"Here, we demonstrated that USP19-CY promotes TGF-beta signaling by directly interacting with TGF-beta type I receptor (TbetaRI) and protecting it from degradation at the plasma membrane."

reach
"Taken together, these results suggest that the catalytic activity of USP19-CY is required to promote TGF-β signaling, while USP19-ER inhibits TGF-β signaling in a DUB activity-independent manner."

reach
"USP19-CY promotes TGF-beta signaling, while USP19-ER exerts the opposite effect."
| 3

reach
"USP19 Promoted GC Cell Proliferation and Metastasis."

reach
"Our results showed that USP19 positively regulated GC cell migration through the up-regulation of MMP2 and MMP9 expression and the decrease of cleaved caspase-3 activity in cell models (XREF_FIG and XREF_FIG)."

reach
"Enhanced USP19 expression promoted GC cell proliferation and metastasis through reduced cleaved caspase-3 and increased MMP2 and MMP9 expression and promoted enzyme activity in the study, and verified the results through The Cancer Genome Atlas (TCGA) and bioinformatic websites from the Kaplan-Meier plotter (http://kmplot.com) and GEPIA (http://gepia2.cancer-pku.cn.)"
USP19 affects SOAT1
| 2 1
USP19 binds SOAT1.
| 1 1
| 1 1

sparser
"Database searches pointed to ubiquitin-specific peptidase 19 (USP19) as the likely SOAT1-specific DUB and this was confirmed by demonstrating a direct interaction between SOAT1 and USP19 in co-immunoprecipitation experiments."

reach
"Database searches pointed to ubiquitin-specific peptidase 19 (USP19) as the likely SOAT1-specific DUB and this was confirmed by demonstrating a direct interaction between SOAT1 and USP19 in co-immunoprecipitation experiments."
USP19 activates SOAT1.
| 1
USP19 activates SOAT1. 1 / 1
| 1

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"Conversely, USP19 over-expression increased SOAT1 and accelerated HCC progression.To further test the hypothesis that USP19 loss inhibits HCC progression in response to p53 loss and that this is related to decreased SOAT1 expression, USP19 and SOAT1 expression was examined in human HCCs in The Cancer Genome Atlas database and confirmed that both messenger RNAs (mRNAs) were up-regulated."
USP19 affects NRF1
| 3
USP19 activates NRF1.
| 2
USP19 activates NRF1. 2 / 2
| 2

sparser
"Also, USP19 activates NRF1 (encoded by Nfe2l1 ), thereby altering glucose deprivation sensing, cholesterol abundance, proteasomal inhibition and oxidative stress [ xref ]."

sparser
"Activation of the membrane-bound Nrf1 transcription factor by USP19, a ubiquitin-specific protease C-terminally anchored in the endoplasmic reticulum."
USP19 binds NRF1.
| 1
| 1

sparser
"Further experiments revealed that USP19 directly interacts with Nrf1 in proximity to the ER and topologically acts as a deubiquitinating enzyme to remove ubiquitin moieties from this protein, which allow it to circumvent potential proteasomal degradation."
USP19 affects NR3C1
| 2 1
USP19 increases the amount of NR3C1.
| 2
USP19 increases the amount of NR3C1. 2 / 2
| 2

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"Thus, USP19 modulates GR levels and in so doing may modulate both insulin and glucocorticoid signaling, two critical pathways that control protein turnover in muscle and overall glucose homeostasis."

reach
"Indeed, overexpression of USP19 not only increased GR levels, but translocation to the nucleus and transcriptional activity of a reporter gene."
| PMC
USP19 binds NR3C1.
| 1
| 1

sparser
"Using a novel BRET assay, we observed that USP19 interacts indirectly with GR through binding with HSP90."
| PMC
USP19 affects MYOG
| 3
USP19 inhibits MYOG.
| 1
USP19 inhibits MYOG. 1 / 1
| 1

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"They found that a decrease of USP19 promoted muscle cell fusion and increased myotube diameter and expressions of myogenin and myosin heavy chain."
USP19 increases the amount of MYOG.
| 1
USP19 increases the amount of MYOG. 1 / 1
| 1

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"The ability of USP19 to modulate expression of myogenin suggests that it might also be involved in myogenesis."
USP19 activates MYOG.
| 1
USP19 activates MYOG. 1 / 1
| 1

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"Our previous observations that silencing USP19 induced expression of myofibrillar proteins as well as myogenin in muscle cells suggested that USP19 might modulate muscle cell differentiation."
USP19 affects MIR7-3HG
| 3
| 3

reach
"The results proved that USP19 overexpression promoted Hep3B and Huh7 cells migration and proliferation and this effect could be recovered by YAP depletion ( Fig. S5A-D )."

reach
"The trans -well and wound healing assays revealed that knockdown of USP19 reduced the migration ability of Hep3B and Huh7 cells ( Fig. 5 A and B)."

reach
"In addition, CCK8 assay also proved that USP19 depletion inhibited the proliferation rate of Hep3B and Huh7 cells ( Fig. 5 E)."
USP19 affects HYCC1
| 3
USP19 activates HYCC1.
| 2
USP19 activates HYCC1. 2 / 2
| 2

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"Furthermore, as mentioned earlier, USP19 has a diverse range of substrates, and its activity is also influenced by multiple signaling pathways, highlighting its involvement in complex cellular process[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"All these results demonstrated that USP19 accelerates HCC progression via YAP.HCC is one of the commonest malignancies in the world, with low survival rates."
USP19 inhibits HYCC1.
| 1
USP19 inhibits HYCC1. 1 / 1
| 1

reach
"Conversely, USP19 over-expression increased SOAT1 and accelerated HCC progression.To further test the hypothesis that USP19 loss inhibits HCC progression in response to p53 loss and that this is related to decreased SOAT1 expression, USP19 and SOAT1 expression was examined in human HCCs in The Cancer Genome Atlas database and confirmed that both messenger RNAs (mRNAs) were up-regulated."
USP19 affects Flag
| 3
| 3

sparser
"Subsequently, Flag-USP19 was transfected into A549 cells."

sparser
"HEK293T cells were transfected with HA-Ub/K48/K63, Myc-YAP, Flag-USP19 or Flag-vector in 10 cm 2 dish."

sparser
"Interestingly, Flag-USP19 did not show any interaction with Myc-REST (Fig.  xref A), suggesting that the effect of USP19 on the reduced REST protein level may be indirect."
| 1

reach
"Thus, USP19-CY promotes TGF-β/SMAD signaling in a DUB-dependent manner."

reach
"100 Loss of USP19 increases transcription of myofibrillar proteins in L6 muscle cells and decreases muscle wasting in response to denervation in mice Priyanka Sundaram 1, Zhiyu Pang 2, Miao Miao 1, Nathalie Bedard 2, Tamara Moore 2, Patricia L. Hallauer 3, Kenneth E. Hastings 3, Simon S. Wing 2 1 Department of Biochemistry, McGill University, Montreal, QC, Canada; 2 Department of Medicine, McGill University, Montreal, QC, Canada; 3 Department of Neurology and Neurosurgery, McGill University, Montreal, QC, Canada Background and aims : Although many enzymes involved in ubiquitination are activated in atrophying muscle, little is known about the role of deubiquitinating enzymes (DUBs)."

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"USP19 modulates the transcription of major myofibrillar proteins, indicating that the ubiquitin system not only mediates increased protein breakdown but also reduces protein synthesis in skeletal musc[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Overexpression of USP19 inhibited RARE transcription and knock-down of USP19 using siRNA showed dramatically increased transcription of RARE (Figure xref and Figure xref ), suggesting that USP19 increases the stability of CORO2A by DUB activity, and that binding of these two protein may be associated with the function of NCoR."
USP19 affects DDIT3
| 3
USP19 increases the amount of DDIT3. 3 / 3
| 3

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"First, USP19 (XREF_FIG) modulated the expression of CHOP, which had previously been shown to be the key marker of the ER stress response that occurs in only a small fraction of the differentiating myoblasts but is critical for differentiation."

reach
"The mechanisms by which CHOP expression is modulated by USP19 will require further study."

reach
"The higher levels of CHOP mRNA seen during regeneration in the USP19 KO muscle suggest that USP19 modulates transcription of CHOP, but modulation of CHOP mRNA stability can not be excluded."
USP19 affects AURKC
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3 |

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Flag affects USP19
| 3
| 3

sparser
"Subsequently, Flag-USP19 was transfected into A549 cells."

sparser
"HEK293T cells were transfected with HA-Ub/K48/K63, Myc-YAP, Flag-USP19 or Flag-vector in 10 cm 2 dish."

sparser
"Interestingly, Flag-USP19 did not show any interaction with Myc-REST (Fig.  xref A), suggesting that the effect of USP19 on the reduced REST protein level may be indirect."
ESR1 affects USP19
| 2 1
ESR1 increases the amount of USP19.
| 2
ESR1 increases the amount of USP19. 2 / 2
| 2

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"These results demonstrate that (i) E2 induces USP19 expression through nuclear ERalpha, (ii) increased USP19 mediated deubiquitinating activity represses myogenesis, and (iii) ERbeta inhibits ERalpha activated USP19 expression."

reach
"A mutant form of ERalpha that is constitutively localized in the nucleus increased USP19 expression and decreased MHC and tropomyosin expression in the presence of E2."
ESR1 binds USP19.
| 1
| 1

sparser
"Previous work has shown that ERα specifically binds to the Usp19 hERE in the presence of E2 in C2C12 cells [17] ."
DNM1L affects USP19
2 | 1
2 | 1

No evidence text available

No evidence text available

sparser
"Interestingly, Chai et al. also found that USP19 could interact with DRP1, although the exact mechanism is still unknown, and whether USP19 promotes the oligomeric transfer of DRP1 to MERCSs requires further investigation ( xref )."
DNAJC5 affects USP19
| 2 1
DNAJC5 binds USP19.
| 1 1
| 1 1

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"Because HSC70 forms a stable complex with its co-chaperone DNAJC5, which was recently implicated in unconventional secretion of Tau and alpha-Syn 15, we also tested whether USP19 could interact with DNAJC5."

sparser
"Because HSC70 forms a stable complex with its co-chaperone DNAJC5, which was recently implicated in unconventional secretion of Tau and α-Syn xref , we also tested whether USP19 could interact with DNAJC5."
DNAJC5 increases the amount of USP19.
| 1
DNAJC5 increases the amount of USP19. 1 / 1
| 1

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"Although DNAJC5 knockdown reduced the expression of USP19 by ~ 30%, this was unlikely to account for the difference in MAPS efficiency between control and DNAJC5 knockdown cells for the following reasons."
3 |
Air Pollutants increases the amount of USP19. 3 / 3
3 |

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No evidence text available
AURKC affects USP19
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3 |

No evidence text available

No evidence text available

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2 |
Titanium dioxide methylates USP19.
1 |

No evidence text available
Titanium dioxide demethylates USP19.
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1 |

No evidence text available
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Sodium arsenite increases the amount of USP19.
1 |
Sodium arsenite increases the amount of USP19. 1 / 1
1 |

No evidence text available
Sodium arsenite decreases the amount of USP19.
1 |
Sodium arsenite decreases the amount of USP19. 1 / 1
1 |

No evidence text available
Rotenone affects USP19
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Rotenone decreases the amount of USP19. 2 / 2
2 |

No evidence text available

No evidence text available
Paracetamol affects USP19
2 |
Paracetamol increases the amount of USP19.
1 |
Paracetamol increases the amount of USP19. 1 / 1
1 |

No evidence text available
Paracetamol decreases the amount of USP19.
1 |
Paracetamol decreases the amount of USP19. 1 / 1
1 |

No evidence text available
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Methacrylaldehyde increases the amount of USP19. 2 / 2
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No evidence text available

No evidence text available
2 |
Aflatoxin B1 increases the amount of USP19. 2 / 2
2 |

No evidence text available

No evidence text available
Acrolein affects USP19
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Acrolein increases the amount of USP19. 2 / 2
2 |

No evidence text available

No evidence text available
XIAP affects USP19
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2 |

No evidence text available

No evidence text available
USP19 affects ubiquitin proteasome
| 2
USP19 activates ubiquitin proteasome. 2 / 2
| 2

reach
"In addition, we can not exclude that p27 Kip1 is also targeted by a non ubiquitin proteasome dependent or independent proteolytic mechanism whose activity is promoted by USP19."

reach
"USP19 suppresses protein synthesis and enhances protein degradation (ubiquitin proteasome system and autophagy) suggesting that it acts on an upstream signalling pathway (s)."
USP19 affects response
| 2
USP19 inhibits response. 2 / 2
| 2

eidos
"The results have shown that USP19 can inhibit the UPR response and weaken CHOP induction , thereby inhibiting muscle cell differentiation ( Figure 2r ) ."

eidos
"Although our results clearly demonstrate that USP19 interacts directly with RORgammat and constrains pathogenic Th17 cell programming and response amplification , deubiquitinases also have effects on multiple other substrates ."
USP19 affects receptor-alpha
| 2
USP19 activates receptor-alpha. 2 / 2
| 2

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"USP19 rescues the ERAD substrates cystic fibrosis transmembrane conductance regulator (CFTR) DeltaF508 and T-cell receptor-alpha (TCRalpha) from proteasomal degradation."

reach
"In preceding studies the ER localised USP19 was shown to rescue the ERAD substrates cystic fibrosis transmembrane conductance regulator (CFTR) DeltaF508 and T-cell receptor-alpha (TCRalpha) from proteasomal degradation 109."
| 2

reach
"Interestingly, silencing of USP19 promotes myoblast fusion in culture and mice lacking USP19 show improved regeneration of muscle following cardiotoxin injury suggesting that the protection from muscl[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"A recent study showed that endoplasmic-reticulum-localized USP19 decreases myoblast fusion during muscle cell differentiation [33] ."
| 2

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"Consistent with our HPLC results, the metabolism of Phe in cells expressing R241C and R243Q was increased by USP19."

reach
"Our results indicate that USP19 promotes Phe metabolism, which was quantified by findings of reduced Phe and increased product Tyr in HPLC and colorimetric assays (Fig. 6)."

reach
"These showed that USP19 loss significantly inhibited lipid metabolism in CRC samples regardless of diet ( Figure 4 D)."

reach
"In a mouse model study of CRC, USP19 and ERK induced the reprogramming of lipid metabolism by regulating the USP19-ME1 signaling pathway."
| 2

reach
"For example, USP3, USP25, and USP18 played a positive regulation of the antiviral immune response [ 5–8 ], while USP5, USP4, USP13, USP15, USP14, USP2b, and USP19 negatively regulated antiviral immune[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"USP19 deficiency enhances T-cell-mediated antitumor immunity by promoting PD-L1 degradation in colorectal cancer."
USP19 affects fusion protein
| 2
USP19 activates fusion protein. 2 / 2
| 2

eidos
"Depletion of USP19 decreased the fusion protein and increased EWS-FLI1 ubiquitination ."

eidos
"Most likely , USP19 , and other DUBs , modulate fusion protein levels to maintain Ewing sarcoma cell viability ."
USP19 affects ferroptosis
| 2
| 2

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"Increased USP19 expression during IRI stabilizes SLC7A11, reduces ferroptosis, and alleviates liver injury (36)."

reach
"Notably, USP19 overexpression reduces ferroptosis and IRI in a SLC7A11-dependent manner in mice."

reach
"USP19 promotes LRP6 biogenesis and controls Wnt signaling."

reach
"Finally, these observations demonstrate that USP19 promotes the biogenesis of LRP6, with only 18% of newly synthesized LRP6 molecules surviving beyond 6 hr in the absence of USP19 and 60% surviving upon USP19 overexpression."
USP19 affects cell death
| 2
| 2

reach
"Based on these observations, we hypothesised that USP19 promotes BC cell death."

reach
"This indicates that the 494–1318 domain of USP19 contains the essential functional properties for misfolded TDP-43 secretion.To ensure that the observed secretion of misfolded TDP-43 was not simply the reflect of cell death potentially induced by the combined expression of USP19-WT and TDP-43-K263E, we assessed cell viability using the trypan blue exclusion method."
USP19 affects cell cycle
| 2
| 2

reach
"The results showed that USP19 knockdown suppressed the proliferation, anchorage-independent growth and xenograft tumor formation of DLBCL cells and arrested the cell cycle at the G1 stage."

reach
"The knockdown of the USP19 gene could inhibit the progression of PCa in two ways; first, by causing defects in cell cycle progression and delaying the progression from G0/G1 to S phase, and second, by accumulating p27 and inhibiting the ability of USP19 to regulate cell growth (Lu et al., 2011)."
USP19 affects XIAP
2 |
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No evidence text available

No evidence text available
USP19 affects Th17
| 2
USP19 inhibits Th17. 2 / 2
| 2

reach
"Strikingly, silencing USP19 has a marginal impact on npTh17 differentiation, measured by the generation of IL-17A-producing cells (Fig. 3F), indicating USP19 selectively suppresses Th17 proinflammatory potential."

reach
"USP19 suppresses Th17-driven pathogenesis in autoimmunity."
USP19 affects TCRalpha
| 2
USP19 increases the amount of TCRalpha.
| 1
Modified USP19 increases the amount of TCRalpha. 1 / 1
| 1

reach
"Usp19 expression increases the levels of TCRalpha and mutant CFTR (cystic fibrosis transconductance regulator) consistent with its role in ERAD regulation."
USP19 activates TCRalpha.
| 1
Mutated USP19 activates TCRalpha. 1 / 1
| 1

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"Interestingly, expression of a catalytically inactive USP19 mutant partially rescued TCRalpha, suggesting a DUB independent role during ERAD."
USP19 affects SQSTM1
1 | 1
USP19 deubiquitinates SQSTM1.
| 1
USP19 deubiquitinates SQSTM1. 1 / 1
| 1

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"Thus, we investigated whether USP19, similar to USP15 and USP17, could deubiquitinate p62."
USP19 binds SQSTM1.
1 |
1 |

No evidence text available
USP19 affects SLC7A11
| 2
| 2

reach
"Mechanistically, USP19 inhibits the degradation of solute carrier family 7 member 11 (SLC7A11) by removing its K63-linked ubiquitin chains."

reach
"Notably, USP19 overexpression reduces ferroptosis and IRI in a SLC7A11-dependent manner in mice."
USP19 affects SIAH
| 2
USP19 binds SIAH. 2 / 2
| 2

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"(1) Constitutive binding partners, often as part of larger macromolecular complexes, e.g., USP22/SAGA-DUBm [167] and CSN5/COP9 signalosome [168] ; (2) low-affinity, transient binding partners, e.g., the USP19/SIAH complex [169] and USP8/NRDP1 [170] , or protein modifiers such as kinases, e.g., USP10/AMPKα [171] ; and (3) ubiquitinated substrates that bind DUBs by virtue of the Ub modification."

reach
"Phylogenetic analysis revealed the SIAH and USP19 complex is conserved in evolution."
USP19 affects RPAP3
2 |
2 |

No evidence text available

No evidence text available
USP19 affects ROR
| 2
USP19 deubiquitinates ROR. 2 / 2
| 2

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"USP19 decreases the ubiquitination level on RORγt-K313, but not that on RORγt-K69 or K446 (Fig. 5C)."

reach
"Therefore, lysine residue 313 could be the major acting site of USP19 to prevent RORγt ubiquitination."
USP19 affects RING1
| 2
USP19 activates RING1. 2 / 2
| 2

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"In addition, USP19 antagonizes ring finger protein 1 (RPF1)-mediated malic enzyme 1 (ME1) degradation through deubiquitination, which in turn promotes lipid metabolism associated with ERK2 activity and CRC development in human patients [139]."

reach
"Ubiquitin-specific peptidase 19 (USP19) antagonizes ring finger protein 1 (RNF1)-mediated ME1 degradation by deubiquitination, thereby promoting lipid metabolism and NADPH production, and suppressing ROS, thereby promoting colorectal carcinogenesis [141]."
USP19 affects PAHwt
| 2
USP19 deubiquitinates PAHwt. 2 / 2
| 2

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"Thus our results indicate that USP19 deubiquitinates PAHwt and PAH variants and rescues them from rapid proteasomal degradation."

reach
"Our results demonstrate that USP19 overexpression reduced the interaction between ubiquitin and PAHwt and the PAH variants, compared with the mock control cells (Fig. 5c)."
USP19 affects PAHwt protein
| 2
USP19 increases the amount of PAHwt protein.
| 1
USP19 increases the amount of PAHwt protein. 1 / 1
| 1

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"In other words, Cdh1 knockdown and USP19 supplementation increased PAHwt protein expression and thereby its enzymatic activity."
USP19 deubiquitinates PAHwt protein.
| 1
USP19 leads to the deubiquitination of PAHwt protein. 1 / 1
| 1

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"In contrast, USP19 reverses the ubiquitination of the PAHwt protein and extends its half-life."

reach
"USP19 Positively Regulates Tumor Growth and Metastasis."

reach
"Furthermore, the authors of this study also showed that USP19 depletion decreased tumor growth and metastasis in vivo."
USP19 affects NADSYN1
| 2
| 1

sparser
"We obtained moderate to strong labeling for Usp19, DNAAF2, and NADSYN1 interactions with AIP and surprisingly strong labeling of AIP–p23 interactions."
| 1

sparser
"In vitro and in situ interactions of Usp19, DNAAF2, NADSYN1, p23/PTGES3, Hsp105 and Bcl‐G with dcTPR proteins."
USP19 affects Mnf2
| 2
USP19 increases the amount of Mnf2.
| 1
USP19 increases the amount of Mnf2. 1 / 1
| 1

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"Knockdown of USP19 decreased the expression of NOX4 protein, promoted the expression of mitochondrial fusion proteins Mnf1 and Mnf2, and inhibited the expression of the splitting protein Drp1."
USP19 decreases the amount of Mnf2.
| 1
USP19 decreases the amount of Mnf2. 1 / 1
| 1

reach
"Knockdown of USP19 decreased the expression of NOX4 protein, promoted the expression of mitochondrial fusion proteins Mnf1 and Mnf2, and inhibited the expression of the splitting protein Drp1."
USP19 affects MAVS
1 | 1
1 | 1

sparser
"Additionally, we found that USP19 specifically interacts with MAVS and deubiquitinates K63-linked ubiquitinated MAVS for negative regulation of type I IFN signaling."

No evidence text available
USP19 affects M
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M binds USP19. 2 / 2
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No evidence text available
| DOI

No evidence text available
| DOI
USP19 affects ISRE
| 2
USP19 inhibits ISRE. 2 / 2
| 2

reach
"As shown in Fig. 8a, USP19 markedly inhibited ISRE activation mediated by wild-type and all of the tested mutants of TRIF, with the exception of TRIF(K523R)."

reach
"Reporter assays indicated that USP19 inhibited ISRE activation mediated by overexpression of TRIF but not TBK1 or IRF3 (Fig. 4a)."
USP19 affects IRF3
2 |
2 |

No evidence text available

No evidence text available
USP19 affects GFP1-10
| 2
USP19 inhibits GFP1-10.
| 1
USP19 inhibits GFP1-10. 1 / 1
| 1

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"Since depletion of USP19 reduced the interaction of GFP1-10 with HSC70, USP19 seems to promote substrate binding by HSC70."
USP19 activates GFP1-10.
| 1
USP19 activates GFP1-10. 1 / 1
| 1

reach
"USP19 CT incrementUI also stimulated GFP1-10 and Tau secretion."
USP19 affects FLVCR1
| 2
USP19 activates FLVCR1. 2 / 2
| 2

reach
"The knockdown of the USP19 gene could inhibit the progression of PCa in two ways; first, by causing defects in cell cycle progression and delaying the progression from G0/G1 to S phase, and second, by accumulating p27 and inhibiting the ability of USP19 to regulate cell growth (Lu et al., 2011)."

reach
"Reports indicate that the disruption of USP19 inhibits a series of PCa cell proliferation by arresting cells in the G1 to S phase transition through stabilization of the cyclin-dependent kinase inhibitor p27 ."
USP19 affects FKBPL
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2 |

No evidence text available

No evidence text available
USP19 affects FKBP8
2 |
2 |

No evidence text available

No evidence text available
USP19 affects FKBP4
2 |
2 |

No evidence text available

No evidence text available
USP19 affects FERD3L
2 |
2 |

No evidence text available

No evidence text available
USP19 affects EWS-FLI1 protein
| 2
USP19 activates EWS-FLI1 protein. 2 / 2
| 2

eidos
"USP19 specifically modulates EWS-FLI1 protein levels ."

eidos
"Depletion of USP19 resulted in diminished EWS-FLI1 protein levels and , vice versa , upregulation of active USP19 stabilized the fusion protein ."
| 2
USP19 deubiquitinates E3_Ub_ligase. 2 / 2
| 2

reach
"USP19 deubiquitinates and stabilizes KPC1, an E3 ligase for p27."

reach
"USP19, constitutively de-ubiquitinates the E3 ligase activity of c-IAP1, thereby inhibiting c-IAP1’s ability to ubiquitinate and target degradation of proteins such as TRAF2 (Figure 4I, Schematic presentation)."
USP19 affects E2F1
| 1 1
| 1 1

sparser
"Dual-luciferase reporter assay and Chromatin Immunoprecipitation (ChIP) assays were used to assess the binding of E2F1 and USP19 promoter."

reach
"Dual-luciferase reporter assay and Chromatin Immunoprecipitation (ChIP) assays were used to assess the binding of E2F1 and USP19 promoter."
USP19 affects DOCK7
1 |
1 |

No evidence text available
USP19 affects DOCK5
2 |
2 |

No evidence text available

No evidence text available
USP19 affects DNAAF2
| 2
| 1

sparser
"We obtained moderate to strong labeling for Usp19, DNAAF2, and NADSYN1 interactions with AIP and surprisingly strong labeling of AIP–p23 interactions."
| 1

sparser
"In vitro and in situ interactions of Usp19, DNAAF2, NADSYN1, p23/PTGES3, Hsp105 and Bcl‐G with dcTPR proteins."
USP19 affects DNA Damage
| 2
| 2

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"20 , 21 USP19 stabilized MGMT and regulated MGMT to mediate DNA methylation damage repair process, further underscoring the dynamic interplay of protein post‐translational modification function on DNA methylation repair."

reach
"USP19 could modulate DNA damage repair by deubiquitinating HDAC2, which resulted in genomic instability and contributed to tumorigenesis [15]."
| 2

reach
"USP19 as a DUB Negatively Regulating Tumorigenesis."

reach
"Conversely, some studies have indicated that USP19 negatively regulates tumorigenesis in ovarian cancer [ 43 ] and clear cell renal cell [ 44 ]."
USP19 affects CRYL1
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No evidence text available

No evidence text available
USP19 affects CDC37L1
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No evidence text available

No evidence text available
USP19 affects CDC37
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No evidence text available

No evidence text available
USP19 affects CD2BP2
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No evidence text available

No evidence text available
TBK1 affects USP19
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No evidence text available

No evidence text available
TARDBP affects USP19
| 1 1
| 1 1

sparser
"TDP-43 is associated with USP19 and the ER compartments and is engulfed in different intracellular compartments."

reach
"Additionally, co-immunoprecipitation experiments demonstrated interactions between USP19 and TDP-43 thus suggesting that part of TDP-43 could be secreted in a form of a tri-complex ER-USP19-TDP-43 engulfed into secretory compartments.To gain a clearer understanding of the cellular mechanisms involved in the misfolded TDP-43 secretion via the MAPS pathway, we evaluated the impact of the Spautin1 and LY294002 inhibitors on the early steps of phagophores/autophagosomes biogenesis [45, 46]."
SOAT1 affects USP19
| 1 1
| 1 1

sparser
"Database searches pointed to ubiquitin-specific peptidase 19 (USP19) as the likely SOAT1-specific DUB and this was confirmed by demonstrating a direct interaction between SOAT1 and USP19 in co-immunoprecipitation experiments."

reach
"Database searches pointed to ubiquitin-specific peptidase 19 (USP19) as the likely SOAT1-specific DUB and this was confirmed by demonstrating a direct interaction between SOAT1 and USP19 in co-immunoprecipitation experiments."
SLC2A1 affects USP19
| 2
SLC2A1 increases the amount of USP19. 2 / 2
| 2

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"The results showed that 15% CSE significantly increased the relative mRNA and protein expression levels of USP19 and FUNDC1 in BEAS-2B cells (Figure 1f–j)."

reach
"In BEAS-2B cells, CSE inhibited cell survival, promoted apoptosis, increased the expression of USP19 and FUNDC1, increased the ratio of LC3 II to LC3 I (LC3 II/I), and decreased mitochondrial membrane potential and TOM20 levels."
SIAH affects USP19
| 2
USP19 binds SIAH. 2 / 2
| 2

reach
"(1) Constitutive binding partners, often as part of larger macromolecular complexes, e.g., USP22/SAGA-DUBm [167] and CSN5/COP9 signalosome [168] ; (2) low-affinity, transient binding partners, e.g., the USP19/SIAH complex [169] and USP8/NRDP1 [170] , or protein modifiers such as kinases, e.g., USP10/AMPKα [171] ; and (3) ubiquitinated substrates that bind DUBs by virtue of the Ub modification."

reach
"Phylogenetic analysis revealed the SIAH and USP19 complex is conserved in evolution."

reach
"ROS inhibits USP19 by acting on the catalytic domain of USP19 (USP19CD) upon treatment with H O while recovered by DTT treatment."

sparser
"ROS inhibits USP19 by acting on the catalytic domain of USP19 (USP19CD) upon treatment with H 2 O 2, while recovered by DTT treatment."
RPAP3 affects USP19
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No evidence text available

No evidence text available
RAR affects USP19
| 2
USP19 binds CORO2A and RAR. 2 / 2
| 2

sparser
"The results suggest that USP19 may be associated with the transcriptional regulation of RAR via CORO2A as one of the components for the NCoR complex during the adipogenesis."

sparser
"These results indicate that USP19 is associated with the regulation of transcriptional repression of RAR through CORO2A."
NOX4 affects USP19
| 1 1
NOX4 deubiquitinates USP19.
| 1
NOX4 deubiquitinates USP19. 1 / 1
| 1

reach
"Deubiquitinating Enzyme USP19 Regulates Ferroptosis and Mitochondrial Damage in SH-SY5Y Cells by Targeting the NOX4 Protein."
NOX4 binds USP19.
| 1
| 1

sparser
"Our study revealed that NOX4 protein interacts with USP19 and knockdown of USP19 enhanced ubiquitination to maintain NOX4 protein stability."
NFE2L1 affects USP19
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No evidence text available

No evidence text available
NADSYN1 affects USP19
| 2
| 1

sparser
"We obtained moderate to strong labeling for Usp19, DNAAF2, and NADSYN1 interactions with AIP and surprisingly strong labeling of AIP–p23 interactions."
| 1

sparser
"In vitro and in situ interactions of Usp19, DNAAF2, NADSYN1, p23/PTGES3, Hsp105 and Bcl‐G with dcTPR proteins."
MAVS affects USP19
1 | 1
1 | 1

sparser
"Additionally, we found that USP19 specifically interacts with MAVS and deubiquitinates K63-linked ubiquitinated MAVS for negative regulation of type I IFN signaling."

No evidence text available
M affects USP19
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M binds USP19. 2 / 2
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No evidence text available
| DOI

No evidence text available
| DOI
LRP6 affects USP19
| 1 1
| 1 1

reach
"Consequently, our results indicate that the functional interaction between USP19 and LRP6 is key for the regulatory effect that USP19 exerts on the modulation of breast cancer cell migration and invasion."

sparser
"Consequently, our results indicate that the functional interaction between USP19 and LRP6 is key for the regulatory effect that USP19 exerts on the modulation of breast cancer cell migration and invasion."
IRF3 affects USP19
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No evidence text available

No evidence text available
HDAC2 affects HDAC1
| 2
| 2

sparser
"USP19 interacts with HDAC1 and HDAC2."

sparser
"Immunoblot analysis showed that both HDAC1 and HDAC2 could bind to USP19 (Figure xref and xref )."
FKBPL affects USP19
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No evidence text available

No evidence text available
FKBP8 affects USP19
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No evidence text available

No evidence text available
FKBP4 affects USP19
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No evidence text available

No evidence text available
FERD3L affects USP19
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No evidence text available

No evidence text available
EV71 affects USP19
| 2
EV71 activates USP19. 2 / 2
| 2

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"Furthermore, EV71 infection induces the upregulation of USP19 which negatively regulates cellular antiviral type I interferon signaling by targeting the tumor necrosis receptor associated factor 3 (TRAF3) molecule and decreasing TRAF3 ubiquitination of K63-linkage [18]."

reach
"Previous studies have indicated that EV71 infection induces the up-regulation of USP19, which negatively regulates the antiviral type I interferon signalling by removing K-63 polyubiquitin chains from TRAF3 35."
ESR affects USP19
| 2
ESR decreases the amount of USP19.
| 1
ESR decreases the amount of USP19. 1 / 1
| 1

reach
"The contributing ratios of daidzein and its metabolites for skeletal muscle health will be an interesting avenue for future work.In the present study, we provide evidence that the ERβ agonist daidzein[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
ESR binds USP19.
| 1
| 1

reach
"Thus, these results indicate that daidzein increases ERβ recruitment to the Usp19 hERE and down-regulates USP19 expression by inhibiting E2-dependent recruitment of ERα."
DOCK7 affects USP19
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No evidence text available
DOCK5 affects USP19
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No evidence text available

No evidence text available
DNAAF2 affects USP19
| 2
| 1

sparser
"We obtained moderate to strong labeling for Usp19, DNAAF2, and NADSYN1 interactions with AIP and surprisingly strong labeling of AIP–p23 interactions."
| 1

sparser
"In vitro and in situ interactions of Usp19, DNAAF2, NADSYN1, p23/PTGES3, Hsp105 and Bcl‐G with dcTPR proteins."
CRYL1 affects USP19
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No evidence text available

No evidence text available
CORO2A affects RAR, and USP19
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USP19 binds CORO2A and RAR. 2 / 2
| 2

sparser
"The results suggest that USP19 may be associated with the transcriptional regulation of RAR via CORO2A as one of the components for the NCoR complex during the adipogenesis."

sparser
"These results indicate that USP19 is associated with the regulation of transcriptional repression of RAR through CORO2A."
CDC37L1 affects USP19
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No evidence text available

No evidence text available
CDC37 affects USP19
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No evidence text available

No evidence text available
CD2BP2 affects USP19
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No evidence text available

No evidence text available

reach
"Purified USP19CD behaved similarly to full-length USP19 as it contained little activity unless treated with DTT (XREF_SUPPLEMENTARY)."

reach
"They further showed that some DUBs that have been exposed to ROS can have different responses to reduction through DTT treatment, with some being activated by DTT (e.g., USP8, USP10, and USP19), some having only enhancement of activity in the presence of DTT (e.g., USP7, CYLD, and UCHL5), and others exhibiting no activity, despite the presence of a reducing agent (e.g., USP1, USP22, and A20) (35)."
Sunitinib affects USP19
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Sunitinib decreases the amount of USP19. 1 / 1
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No evidence text available
ShUSP19-1 affects USP19
| 1
ShUSP19-1 inhibits USP19. 1 / 1
| 1

reach
"The USP19 mRNA and protein expression levels were significantly decreased by shUSP19-1, -2, and -3 in BEAS-2B cells (Figure 2a–c)."
Saxitoxin affects USP19
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Saxitoxin decreases the amount of USP19. 1 / 1
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No evidence text available
Perfluorooctanesulfonamide decreases the amount of USP19. 1 / 1
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No evidence text available
P97 affects USP19
| 1
USP19 binds p97. 1 / 1
| 1

sparser
"In addition, it is also unclear whether or not USP19 can interact with p97."
| 1

reach
"After overexpressing USP19 and BAG6, the endogenous BCL2 protein levels were reduced in MDA‐MB‐231 cells (Figure 6D)."
Ivermectin affects USP19
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Ivermectin decreases the amount of USP19. 1 / 1
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No evidence text available
Inducible shUSP19 constructs affects USP19
| 1
Inducible shUSP19 constructs inhibits USP19. 1 / 1
| 1

eidos
"To this end , we established SKNMC and A673 cells stably expressing two inducible shUSP19 constructs which allow to deplete USP19 by addition of doxycycline ."
1 |
Hsa-miR-196a-5p decreases the amount of USP19. 1 / 1
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No evidence text available
| 1
| 1

sparser
"We found that palmitic acid elevated palmitoylation of USP19 (Figure  xref , Supporting Information) and reduced ACSL4 protein level (Figure  xref , Supporting Information), and these effects disappeared after ZDHHC2 knockout (Figure  xref , Supporting Information)."
Hexabromocyclododecane increases the amount of USP19. 1 / 1
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No evidence text available
Gallic acid affects USP19
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Gallic acid increases the amount of USP19. 1 / 1
1 |

No evidence text available
Furan affects USP19
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Furan methylates USP19. 1 / 1
1 |

No evidence text available
Folic acid affects USP19
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Folic acid increases the amount of USP19. 1 / 1
1 |

No evidence text available
1 |
Deltamethrin decreases the amount of USP19. 1 / 1
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No evidence text available
Cadmium dichloride decreases the amount of USP19. 1 / 1
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No evidence text available
1 |
Arsenite(3-) decreases the amount of USP19. 1 / 1
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No evidence text available
Aristolochic acid A increases the amount of USP19. 1 / 1
1 |

No evidence text available
XRN2 affects USP19
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No evidence text available
VDAC1 affects USP19
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No evidence text available
Ubiquitin affects USP19
| 1
| 1

sparser
"As the main ubiquitin acceptor site for EWS-FLI1 degradation is located in the FLI1 terminus xref , we were next interested in identifying whether USP19 still interacts with EWS-FLI1 ubiquitin acceptor site mutants."
USP7 affects USP19
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No evidence text available
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No evidence text available
USP19 affects α-syn
| 1
USP19 leads to the deubiquitination of α-syn. 1 / 1
| 1

reach
"Since Lewy body inclusions are known to be ubiquitinated, we tested whether loss of the USP19 deubiquitinase might increase ubiquitination of α-syn."
USP19 affects vice active USP19 stabilized fusion protein
| 1
USP19 activates vice active USP19 stabilized fusion protein. 1 / 1
| 1

eidos
"Depletion of USP19 resulted in diminished EWS-FLI1 protein levels and , vice versa , upregulation of active USP19 stabilized the fusion protein ."
USP19 affects ubiquitination RORgammat-K313 RORgammat-K69 K446
| 1
USP19 inhibits ubiquitination RORgammat-K313 RORgammat-K69 K446. 1 / 1
| 1

eidos
"USP19 decreases the ubiquitination level on RORgammat-K313 , but not that on RORgammat-K69 or K446 ( Fig. 5C ) ."
USP19 affects ubiquitin peptidase
| 1
USP19 activates ubiquitin peptidase. 1 / 1
| 1

reach
"Here we found that USP19 overexpression significantly promotes the secretion of soluble and aggregated misfolded TDP-43, requiring both ER anchoring and ubiquitin peptidase activity."
USP19 affects translation
| 1
| 1

reach
"The ability of USP19 to modulate protein synthesis, degradation as well as myogenesis suggest that it is modulating a signaling pathway(s) that is upstream of these processes."
USP19 affects transactivity
| 1
USP19 activates transactivity. 1 / 1
| 1

eidos
"Herein , we found that Nrf1 stability and transactivity are both enhanced by USP19 , a ubiquitin-specific protease tail-anchored in the endoplasmic reticulum ( ER ) through its C-terminal transmembrane domain ."
| 1
| 1

reach
"These results suggested that USP19 expression enhanced TMZ stability and induced resistance in GBM, which led to patients having poor treatment outcomes.3 DISCUSSION."

reach
"We demonstrated that USP19 deficiency significantly enhances the susceptibility to T cell-mediated cytotoxicity in CRC cells, organoids, and mouse models."
USP19 affects suggesting have oncogenic properties33
| 1
USP19 activates suggesting have oncogenic properties33. 1 / 1
| 1

eidos
"There , USP19 depletion inhibited proliferation of prostate cancer and breast epithelial cell lines suggesting it to have oncogenic properties33 ,34 ."
USP19 affects stability MARCHF6
| 1
USP19 activates stability MARCHF6. 1 / 1
| 1

eidos
"Interestingly , USP19 , an ER-resident DUB , and cholesterol enhance the stability of MARCHF6 [ 250,251 ] ."
USP19 affects several mitotic defects including cytokinesis
| 1
USP19 activates several mitotic defects including cytokinesis. 1 / 1
| 1

eidos
"Targeted genome knockout verified that USP19 depletion leads to several mitotic defects , including cytokinesis failure ."
USP19 affects self-ubiquitin ligase
| 1
USP19 inhibits self-ubiquitin ligase. 1 / 1
| 1

reach
"These results suggest that the self-ubiquitin ligase activity of c-IAPs is inhibited by USP19 and implicate deubiquitinating enzymes in the regulation of IAP stability."

reach
"USP19 also interacts with HIF-1α, promoting its stability and an appropriate hypoxia response ( Altun et al., 2012 )."
USP19 affects prostate cancer breast epithelial cell lines
| 1
USP19 activates prostate cancer breast epithelial cell lines. 1 / 1
| 1

eidos
"There , USP19 depletion inhibited proliferation of prostate cancer and breast epithelial cell lines suggesting it to have oncogenic properties33 ,34 ."
USP19 affects polyubiquitination PAH
| 1
USP19 inhibits polyubiquitination PAH. 1 / 1
| 1

eidos
"Our study indicates that USP19 interacts with PAH and prevents polyubiquitination of PAH subsequently extending the half-life of PAH protein ."
USP19 affects polyubiquitin chains
| 1
USP19 inhibits polyubiquitin chains. 1 / 1
| 1

reach
"In addition, USP19 inhibited the proteasomal degradation of inflammasome independent NLRP3 by cleaving its polyubiquitin chains."
USP19 affects pathogenic potential
| 1
USP19 inhibits pathogenic potential. 1 / 1
| 1

eidos
"Taken together , USP19 suppresses pTh17 differentiation and pathogenic potential through the removal of K63-linked ubiquitin chain from lysine 313 of RORgammat , which is required for recruiting coactivator SRC3 and essential for the expression of proinflammatory effectors ( Fig. 6E ) ."
| 1
| 1

reach
"Hence, pioneering researches rarely focused on whether and how hypoxia regulates the Beclin-1 expression in cervical cancer.In this study, we found that hypoxia will induce significant decrease of bot[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP19 affects pTh17 differentiation
| 1
USP19 inhibits pTh17 differentiation. 1 / 1
| 1

eidos
"Taken together , USP19 suppresses pTh17 differentiation and pathogenic potential through the removal of K63-linked ubiquitin chain from lysine 313 of RORgammat , which is required for recruiting coactivator SRC3 and essential for the expression of proinflammatory effectors ( Fig. 6E ) ."
USP19 affects p97
| 1
USP19 binds p97. 1 / 1
| 1

sparser
"In addition, it is also unclear whether or not USP19 can interact with p97."

reach
"Additionally, under physiological conditions in young female mice, E2 induces USP19 expression through ERα, and knockdown of either ERα or USP19 increases soleus muscle mass by inducing muscle hypertr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
| 1
| 1

reach
"We therefore tested whether USP19 modulates this differentiation dependent CHOP induction and, if so, whether it requires ER localization."
USP19 affects lipogenesis colorectal carcinogenesis
| 1
USP19 activates lipogenesis colorectal carcinogenesis. 1 / 1
| 1

eidos
"USP19 exacerbates lipogenesis and colorectal carcinogenesis by stabilizing ME1 ."
USP19 affects hypoxia-induced mitochondrial division
| 1
USP19 activates hypoxia-induced mitochondrial division. 1 / 1
| 1

eidos
"USP19 promotes hypoxia-induced mitochondrial division via FUNDC1 at ER-mitochondria contact sites ."
USP19 affects hypertrophic
| 1
USP19 inhibits hypertrophic. 1 / 1
| 1

eidos
"Consistently , gain-of-function and loss-of-function approaches that involved USP19 in cardiomyocytes suggested that the down-regulation of USP19 promoted the hypertrophic phenotype , while the up-regulation of USP19 improved the worsened phenotype ."
USP19 affects growth tumors mouse xenograft
| 1
USP19 activates growth tumors mouse xenograft. 1 / 1
| 1

eidos
"Additionally , USP19 depletion results in significant enrichment of apoptosis and reduces the growth of tumors in the mouse xenograft ."
USP19 increases the amount of green fluorescent protein. 1 / 1
| 1

reach
"Surprisingly, USP19 but not USP7 significantly increased the secretion of green fluorescent protein (GFP) (expressed as a cytosolic control protein) with no detectable effect on cell permeability (Fig. 1b–d)."
USP19 affects glutathione
| 1
| 1

reach
"Furthermore, USP19 knockdown decreased mitochondrial membrane potential, SOD, MDA, intracellular iron content and increased GSH/GSSG ratio in SH-SY5Y cells."
USP19 affects extending half-life PAH protein
| 1
USP19 inhibits extending half-life PAH protein. 1 / 1
| 1

eidos
"Our study indicates that USP19 interacts with PAH and prevents polyubiquitination of PAH subsequently extending the half-life of PAH protein ."
USP19 affects exocytosis
| 1
| 1

reach
"We found that loss of USP19 increased the levels of ubiquitinated p62 and reduced lysosomal exocytosis under serum starvation condition, suggesting that USP19 may regulate lysosomal exocytosis by a similar mechanism."
USP19 affects enzymatic PAH
| 1
USP19 activates enzymatic PAH. 1 / 1
| 1

eidos
"USP19 increases the enzymatic activity of PAH , thus maintaining normal Phe levels ."
USP19 affects dioxygen
| 1
| 1

reach
"USP19 inhibited NLRP3 inflammasome activation by increasing autophagy flux and decreasing the generation of mitochondrial reactive oxygen species."
| 1
| 1

reach
"Depletion of USP19 reversed the dexamethasone suppression of MHC."
USP19 affects cellular migration
| 1
USP19 activates cellular migration. 1 / 1
| 1

eidos
"Our in vitro validation experiments showed that USP19 depletion did not affect cell proliferation in agreement with Lu et al.64 , but directly inhibited cellular migration ."

reach
"To identify the subcellular level at which USP19 regulates ME1 stability, USP19-depleted HCT116 cells were treated with the proteasome inhibitor MG132, the lysosome inhibitor NH 4 Cl, or the autophagy[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"USP19 potentiates autophagic cell death via inhibiting mTOR pathway through deubiquitinating NEK9 in pancreatic cancer."
USP19 affects XRN2
1 |
1 |

No evidence text available
USP19 affects VDAC1
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1 |

No evidence text available
USP19 affects VAMP7
| 1
USP19 activates VAMP7. 1 / 1
| 1

reach
"Overexpression of GFP-YKT6-WT or GFP-YKT6-Longin did not impair the USP19-mediated TDP-43-K263E secretion, indicating a specific impact of VAMP7 in USP19-mediated secretion (Fig. 7d, e)."
| 1

eidos
"USP19 binds to the EWSR1 domain of the fusion protein , as demonstrated by co-immunoprecipitation experiments , and reduces ubiquitination at an acceptor lysine site ( the K380 residue35 ) in the FLI1 domain ."
USP19 affects USP7
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No evidence text available
USP19 affects USP19-ME1
| 1
USP19 activates USP19-ME1. 1 / 1
| 1

reach
"In a mouse model study of CRC, USP19 and ERK induced the reprogramming of lipid metabolism by regulating the USP19-ME1 signaling pathway."
USP19 affects USP
| 1
USP19 binds USP. 1 / 1
| 1

sparser
"As the USP domain of USP19 binds Hsp90 ( xref ) xref , the detected chaperoning activity could be attributed to either Hsp90 or the USP domain itself."
USP19 affects UQCC2
| 1
USP19 decreases the amount of UQCC2. 1 / 1
| 1

reach
"Knockdown of USP19 decreased the expression of NOX4 protein, promoted the expression of mitochondrial fusion proteins Mnf1 and Mnf2, and inhibited the expression of the splitting protein Drp1."
USP19 affects UPS19
| 1
USP19 activates UPS19. 1 / 1
| 1

reach
"Collectively, these data suggest that USP19 regulates microglial M1/M2 polarization via FOXO1/IL-10/IL-10R1 axis in vitro.3.6 Low-dose LPS upregulates UPS19 to modulate microglial M1/M2 polarization in SAH mice."
USP19 affects UBL
| 1
USP19 binds SLAMF7 and UBL. 1 / 1
| 1

sparser
"We found that the tandem CS domains fold back to interact with the C-terminal USP domain (USPD) intra-molecularly that leads to inhibition of the catalytic core of USP19, especially CS1 interacts with the embedded UbL domain and CS2 does with the CH2 catalytic core."

reach
"Moreover, USP19 (induced by TIPE3) triggers macrophage M2 polarization in the tumor microenvironment, inhibiting the immune response and affecting autophagy and apoptosis of tumor cells (Fig. 8)."
USP19 affects Th17-driven pathogenesis autoimmunity T helper
| 1
USP19 inhibits Th17-driven pathogenesis autoimmunity T helper. 1 / 1
| 1

eidos
"USP19 suppresses Th17-driven pathogenesis in autoimmunity T helper 17 ( Th17 ) cells have emerged as a chief pathogenic cell type in murine models of autoimmunity and human autoimmune diseases ."
USP19 affects TRAF6
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No evidence text available
USP19 affects TOMM20
| 1
USP19 increases the amount of TOMM20. 1 / 1
| 1

reach
"In CSE-treated BEAS-2B cells, USP19 knockdown reduced FUNDC1 and LC3 II/I, increased the levels of TOM20, improved cell survival, mitochondrial membrane potential, and OCR, and inhibited apoptosis."
USP19 affects TMEM33
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No evidence text available
USP19 affects TLR3
| 1
USP19 inhibits TLR3. 1 / 1
| 1

reach
"These results suggest that USP19 impairs the association of TRIF with TLR3 after TLR3 activation in a deubiquitinating enzymatic activity dependent manner.Similarly, we found that overexpression of USP19 but not USP19(C607S) inhibited the association between TRIF and TLR4 (Fig. 6d)."
USP19 affects TGFBR1
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No evidence text available
USP19 affects SYNM
| 1
USP19 activates SYNM. 1 / 1
| 1

reach
"Using a murine model expressing the disease associated α-syn A53T mutation, we demonstrated that loss of USP19 clearly decreases the burden of pSyn-positive aggregates in the brains injected with α-syn PFF."
USP19 affects SUCLG2
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No evidence text available
USP19 affects STIP1
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No evidence text available
USP19 affects SRPRB
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1 |

No evidence text available
USP19 affects SNRNP200
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No evidence text available
USP19 affects SMAD2
| 1
USP19 leads to the phosphorylation of SMAD2. 1 / 1
| 1

reach
"To examine the mechanism underlying the USP19-CY-induced promotion of TGF-β/SMAD signaling, we investigated whether USP19-CY targets TβRI, as USP19-CY stimulates TGF-β-induced SMAD2 phosphorylation."
USP19 affects SLC6A4
| 1
USP19 increases the amount of SLC6A4. 1 / 1
| 1

reach
"The interaction of USP19 with HSP90 accelerates the aggregation of HTT by modulating the protein level of HTT, confirming that the effect of USP19 decreases when the interaction between HTT and HSP90 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP19 affects SLC27A6
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No evidence text available
USP19 affects SLC1A5
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No evidence text available
USP19 affects SLC16A9
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No evidence text available
USP19 affects SLAMF7
| 1
USP19 binds SLAMF7 and UBL. 1 / 1
| 1

sparser
"We found that the tandem CS domains fold back to interact with the C-terminal USP domain (USPD) intra-molecularly that leads to inhibition of the catalytic core of USP19, especially CS1 interacts with the embedded UbL domain and CS2 does with the CH2 catalytic core."
USP19 affects SKP2
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1 |

No evidence text available
USP19 affects SFTPC
| 1
USP19 decreases the amount of SFTPC. 1 / 1
| 1

reach
"Our screening results indicated that the loss of USP11 decreased SFTPC protein levels when compared with the mock control, while the loss of USP19 increased SFTPC expression (Figure 1B)."
USP19 affects SEL1L
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1 |

No evidence text available
USP19 affects SAH mice
| 1
USP19 activates SAH mice. 1 / 1
| 1

reach
"Collectively, these data suggest that USP19 regulates microglial M1/M2 polarization via FOXO1/IL-10/IL-10R1 axis in vitro.3.6 Low-dose LPS upregulates UPS19 to modulate microglial M1/M2 polarization in SAH mice."
USP19 affects SAAL1
1 |
1 |

No evidence text available

reach
"In terms of mechanisms, USP19 increases autophagic flux and reduces mitochondrial ROS production, thus inhibiting inflammation and promoting macrophage polarization in M2 (133) (Fig. 3)."
USP19 affects RNF2
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No evidence text available
USP19 affects Population
| 1
| 1

eidos
"In contrast , reducing USP19 notably fostered pTh17 differentiation ( Fig. 3C and Supplementary Fig. 2A ) , increasing IL-17A + IFN-gamma + population by approximately 2-fold ."
USP19 affects PYGL
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1 |

No evidence text available
USP19 affects PPP2R5B
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1 |

No evidence text available
USP19 affects PINK1
| 1
USP19 inhibits PINK1. 1 / 1
| 1

reach
"Moreover, USP19 knockdown alleviated HG+FFA-mediated PTEN-induced putative kinase 1 (PINK1)/Parkin pathway inactivation and increased mitochondrial reactive oxygen species (ROS) generation in HK-2 cells."
USP19 affects PARK7
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1 |

No evidence text available
USP19 affects OTULINL
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No evidence text available
USP19 affects NUP160
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No evidence text available
USP19 affects NOX4
| 1
| 1

sparser
"Our study revealed that NOX4 protein interacts with USP19 and knockdown of USP19 enhanced ubiquitination to maintain NOX4 protein stability."
USP19 affects NLRP3 inflammasome
| 1
USP19 inhibits NLRP3 inflammasome. 1 / 1
| 1

eidos
"USP19 inhibited NLRP3 inflammasome activation by increasing autophagy flux and decreasing the generation of mitochondrial reactive oxygen species ."
USP19 affects NH
| 1
USP19 activates NH. 1 / 1
| 1

reach
"To identify the subcellular level at which USP19 regulates ME1 stability, USP19-depleted HCT116 cells were treated with the proteasome inhibitor MG132, the lysosome inhibitor NH 4 Cl, or the autophagy[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP19 affects NFkappaB
| 1
| 1

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"For instance, USP19 inhibits the degradation of TGF-beta activated kinase 1, thereby modulating the NF-kB signaling pathway and immune responses (4)."
USP19 affects NFE2L2
| 1
USP19 inhibits NFE2L2. 1 / 1
| 1

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"The results revealed that USP19 knockdown dramatically increased NRF2 and PD-L1 protein through induced reduction of NADPH/NADP + ratio and increase of ROS levels in both HCT116 and DLD1 cells ( Figur[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP19 affects NFASC
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USP19 activates NFASC. 1 / 1
| 1

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"So an attractive hypothesis is that USP19 promotes muscle atrophy by stabilizing cIAPs and activating the NFκB pathway."
USP19 affects NEK9
| 1
USP19 binds NEK9 and K48. 1 / 1
| 1

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"Mechanistically, USP19 directly interacts with and stabilized NEK9 via inhibiting K48-specific polyubiquitination process on NEK9 protein at K525 site through its USP domain."
USP19 affects NEDD4
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USP19 activates NEDD4. 1 / 1
| 1

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"In addition, USP19 antagonizes ring finger protein 1 (RPF1)-mediated malic enzyme 1 (ME1) degradation through deubiquitination, which in turn promotes lipid metabolism associated with ERK2 activity and CRC development in human patients [139]."
USP19 affects Mhc
| 1
USP19 increases the amount of Mhc. 1 / 1
| 1

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"Depletion of all isoforms of USP19 increased myogenin (XREF_FIG) and tropomyosin and MHC protein levels (XREF_FIG), as previously reported, and enhanced myotube formation and fusion (XREF_FIG)."
USP19 affects MRPL42
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No evidence text available
USP19 affects MAPT
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USP19 activates MAPT. 1 / 1
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"USP19 CT incrementUI also stimulated GFP1-10 and Tau secretion."
USP19 affects LRPPRC
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No evidence text available
USP19 affects LMBR1L
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No evidence text available
USP19 affects LGMN
| 1
USP19 increases the amount of LGMN. 1 / 1
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"Knockdown of USP19 in all these cell lines reduced extracellular levels of LGMN, without affecting their levels in the lysates (Fig. 6, C–F)."
USP19 affects LAT
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1 |

No evidence text available
USP19 affects KPTN
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1 |

No evidence text available
USP19 affects K63-
| 1
USP19 binds K63- and K27. 1 / 1
| 1

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"Mechanistically, USP19 interacted with TAK1 in a TNF-alpha- or IL-1beta-dependent manner and specifically deconjugated K63- and K27 linked polyubiquitin chains from TAK1, leading to the impairment of TAK1 activity and the disruption of the TAK1 and TAB2/3 complex."
USP19 affects K48
| 1
USP19 binds NEK9 and K48. 1 / 1
| 1

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"Mechanistically, USP19 directly interacts with and stabilized NEK9 via inhibiting K48-specific polyubiquitination process on NEK9 protein at K525 site through its USP domain."
USP19 affects K29
| 1
| 1

sparser
"For example, we identified USP32 as a K6 and K29 diUbs selective interactor, while USP19 selectively interacts with K29 diUb."
USP19 affects K27
| 1
USP19 binds K63- and K27. 1 / 1
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"Mechanistically, USP19 interacted with TAK1 in a TNF-alpha- or IL-1beta-dependent manner and specifically deconjugated K63- and K27 linked polyubiquitin chains from TAK1, leading to the impairment of TAK1 activity and the disruption of the TAK1 and TAB2/3 complex."

reach
"Moreover, Usp19 deficiency reduces insulin resistance in the liver and skeletal muscle [49]."
USP19 affects IL10RA
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USP19 activates IL10RA. 1 / 1
| 1

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"As presented in Fig. 5A, USP19 overexpression led to induction of USP19, FOXO1, IL-10 and IL-10R1 in SH-SY5Y cells."
USP19 affects IL1
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USP19 inhibits IL1. 1 / 1
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"Recent study has demonstrated that USP19 negatively regulates TNF-α- and IL-1β-activated NF-κB signaling via deubiquinating TAK1 [14]."
USP19 affects IARS1
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No evidence text available
USP19 affects Hypoxia
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| 1

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"We found that upon diverse oxygen concentration treatment, the USP19 mRNA also dramatically reduced at 6 h post hypoxia challenge in HeLa cells, and it also decreased as hypoxia was prolonged ( Figs."
USP19 affects Hypertrophy
| 1
| 1

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"The USP19 attenuates pathological cardiac hypertrophy by inhibiting the TAK1-dependent pathway (Miao et al. 2020), and in addition, Tang et al. found that USP7 reduces myocardial ischemia-reperfusion injury by regulating ferroptosis (Tang et al. 2021), suggesting an important role for the presence of USP in the heart."
USP19 affects Htt-N90
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USP19 activates Htt-N90. 1 / 1
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"Modulation of Htt-N90 by USP19 is dependent on the interaction with HSP90."
USP19 affects HSPH1
| 1
| 1

sparser
"In vitro and in situ interactions of Usp19, DNAAF2, NADSYN1, p23/PTGES3, Hsp105 and Bcl‐G with dcTPR proteins."
USP19 affects HSPA1A
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No evidence text available
USP19 affects HSP90AB1
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No evidence text available
USP19 affects HNRNPAB
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No evidence text available
USP19 affects HERC2
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No evidence text available

reach
"As to its essential role in DNA repair and in maintaining chromosome stability, USP19 might be critical for cells to prevent genome instability and its deletion might be contributed to tumorigenesis."
USP19 affects GTF2H1
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USP19 decreases the amount of GTF2H1. 1 / 1
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"Western blotting and immunofluorescence experiments of proteins related to autophagy showed that USP19 overexpression substantially reduced the protein expression of P62 in LoVo and SW480 cells while significantly elevating the protein expression of Beclin1 and LC3, as compared to the L-OHP group (Fig. 4B–D)."
USP19 affects GST
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sparser
"BAG6 was subsequently treated with purified GSTUSP19 protein that had been immobilised on protein G beads (Figure  xref )."
USP19 affects GCN1
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No evidence text available
USP19 affects G3BP1
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No evidence text available
USP19 affects FKBP51
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AIP binds FKBP51 and USP19. 1 / 1
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sparser
"We confirmed interaction of ubiquitin carboxypeptidase Usp19 with two dcTPR proteins, AIP and FKBP51, via the dicarboxylate clamp mechanism."
USP19 affects EWSR1-FLI1
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USP19 activates EWSR1-FLI1. 1 / 1
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eidos
"Depletion of USP19 was shown to decrease EWSR1-FLI1 expression levels , but neither the expression of wild-type of EWSR1 nor FLI1 , and to reduce tumor growth in vitro and in vivo indicating a potential therapeutic value for USP19 inhibition [ 101 ] ."
USP19 affects EWS-FLI1
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USP19 activates EWS-FLI1. 1 / 1
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"Even though USP19 specifically modulated EWS-FLI1 but not EWSR1 protein levels ( Fig. 2e ) , we were still interested if USP19 possibly alters EWSR1 monoubiquitination levels35 ."
USP19 affects EWS-FLI1 fusion oncoprotein
| 1
USP19 deubiquitinates EWS-FLI1 fusion oncoprotein. 1 / 1
| 1

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"USP19 supports normal cell proliferation through stabilizing KPC1, inhibits tumor necrosis factor alpha (TNFα)-induced apoptosis by stabilizing c-inhibitors of apoptosis (IAPs) and regulates Ewing sar[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP19 affects ESR1
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| 1

sparser
"Previous work has shown that ERα specifically binds to the Usp19 hERE in the presence of E2 in C2C12 cells [17] ."
USP19 affects ESR
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| 1

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"Thus, these results indicate that daidzein increases ERβ recruitment to the Usp19 hERE and down-regulates USP19 expression by inhibiting E2-dependent recruitment of ERα."
USP19 affects ERAD machinery factors
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USP19 binds ERAD machinery factors. 1 / 1
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"Intriguingly, although overexpressed USP19 interacts with Derlin-1 and other ERAD machinery factors in the membrane, endogenous USP19 is mostly in the cytosol where it binds Hsp90."
USP19 affects Death
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USP19 inhibits Death. 1 / 1
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"Deficiency of USP19 increased serum cytokine levels after administration of poly(I:C) and LPS, and promoted inflammatory death caused by administration of poly(I:C) or LPS, or infection of Salmonella typhimurium."
USP19 affects DET1
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No evidence text available
USP19 affects DENR
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USP19 decreases the amount of DENR. 1 / 1
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"Knockdown of USP19 decreased the expression of NOX4 protein, promoted the expression of mitochondrial fusion proteins Mnf1 and Mnf2, and inhibited the expression of the splitting protein Drp1."
USP19 affects DCTN3
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No evidence text available
USP19 affects CUL4B
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No evidence text available
USP19 affects CUL4A
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No evidence text available
USP19 affects CFTRDeltaF508
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USP19 activates CFTRDeltaF508. 1 / 1
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"A catalytically inactive USP19 was still able to partly rescue TCRalpha but not CFTRDeltaF508, suggesting that USP19 might also exert a non catalytic function on specific ERAD substrates."
USP19 affects CFTR ER-associated degradation
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USP19 inhibits CFTR ER-associated degradation. 1 / 1
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"CFTR ubiquitylation at the ER is reversible , and USP19 , an ER membrane tail-anchored de-ubiquitylating enzyme ( DUB ) , has been reported to suppress the CFTR ER-associated degradation ( ERAD ) [ 33 ] ."
USP19 affects CDC123
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No evidence text available
USP19 affects CD274
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USP19 inhibits CD274. 1 / 1
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"The results revealed that USP19 knockdown dramatically increased NRF2 and PD-L1 protein through induced reduction of NADPH/NADP + ratio and increase of ROS levels in both HCT116 and DLD1 cells ( Figur[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
| 1

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"Interestingly, Usp19 also interacts with CaMK-II and since alphakap forms complexes with CaMK-II, it is possible that alphakap may function as a scaffold for Usp19."
USP19 affects CAMK2G
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No evidence text available
| 1

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"We demonstrated that USP19 positively regulates breast tumor cells migration and invasion in vitro, and that genetic silencing reduces cells motility, whereas its overexpression increases migratory and invasive capabilities—dependent on USP19’s catalytic activity and ER localization."
USP19 affects Body Weight
| 1
| 1

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"USP19 whole body KO animals have decreased body weight (~20% reduced in females, ~10% in males ), mainly due to an ~60% reduction in the adipose tissue (ref."
USP19 affects B4GALT2
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No evidence text available

sparser
"We also found that five CsPIN protein members (PIN1, PIN1b, PIN1c, PIN2, and PIN3) in cucumber may interact with ARF and USP19 ( xref )."
USP19 affects ARL8B
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No evidence text available
USP19 affects APEX1
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No evidence text available
USP19 affects A549
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USP19 binds BIRC5 and A549. 1 / 1
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"Therefore, we first verified the potential effects of gastrodin on the interaction between USP19 and survivin in A549 cells."
USP affects USP19
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USP19 binds USP. 1 / 1
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sparser
"As the USP domain of USP19 binds Hsp90 ( xref ) xref , the detected chaperoning activity could be attributed to either Hsp90 or the USP domain itself."
UBL affects USP19
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USP19 binds SLAMF7 and UBL. 1 / 1
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"We found that the tandem CS domains fold back to interact with the C-terminal USP domain (USPD) intra-molecularly that leads to inhibition of the catalytic core of USP19, especially CS1 interacts with the embedded UbL domain and CS2 does with the CH2 catalytic core."
TRAF6 affects USP19
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No evidence text available
TMEM33 affects USP19
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No evidence text available
TGFBR1 affects USP19
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No evidence text available
| 1

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"USP19 Promoted GC Cell Proliferation and Metastasis."
Smoke affects USP19
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Smoke decreases the amount of USP19. 1 / 1
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No evidence text available
SUCLG2 affects USP19
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No evidence text available
STIP1 affects USP19
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No evidence text available
SRPRB affects USP19
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1 |

No evidence text available
SQSTM1 affects USP19
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1 |

No evidence text available
SNRNP200 affects USP19
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No evidence text available
SLC27A6 affects USP19
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1 |

No evidence text available
SLC1A5 affects USP19
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1 |

No evidence text available
SLC16A9 affects USP19
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1 |

No evidence text available
SLAMF7 affects USP19
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USP19 binds SLAMF7 and UBL. 1 / 1
| 1

sparser
"We found that the tandem CS domains fold back to interact with the C-terminal USP domain (USPD) intra-molecularly that leads to inhibition of the catalytic core of USP19, especially CS1 interacts with the embedded UbL domain and CS2 does with the CH2 catalytic core."
SKP2 affects USP19
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1 |

No evidence text available
SEL1L affects USP19
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1 |

No evidence text available
SAAL1 affects USP19
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1 |

No evidence text available
Reperfusion Injury increases the amount of USP19. 1 / 1
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"As illustrated in Figures XREF_FIG and XREF_FIG, the I/R procedure remarkably increased the protein levels of USP19, Beclin1, NCOA4, and LC3II and LC3I."
Raloxifene Hydrochloride decreases the amount of USP19. 1 / 1
1 |

No evidence text available
RNF2 affects USP19
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1 |

No evidence text available
PYGL affects USP19
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1 |

No evidence text available
PPP2R5B affects USP19
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1 |

No evidence text available
PFF affects USP19
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PFF activates USP19. 1 / 1
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"PFF treatment led to ~20% cell death at 10 dpt in both USP19 WT and KO neurons (Fig. 5c)."
PARK7 affects USP19
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No evidence text available
OTULINL affects USP19
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1 |

No evidence text available
NUP160 affects USP19
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No evidence text available
NRF1 affects USP19
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| 1

sparser
"Further experiments revealed that USP19 directly interacts with Nrf1 in proximity to the ER and topologically acts as a deubiquitinating enzyme to remove ubiquitin moieties from this protein, which allow it to circumvent potential proteasomal degradation."
NR3C1 affects USP19
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sparser
"Using a novel BRET assay, we observed that USP19 interacts indirectly with GR through binding with HSP90."
| PMC
NLRP3 affects USP19
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sparser
"Therefore, this study aims to elucidate the role of USP19 in EOC and to determine whether modulating the USP19-NLRP3 signaling could represent a novel therapeutic approach for targeting TAMs in ovarian cancer."
NEK9 affects USP19
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USP19 binds NEK9 and K48. 1 / 1
| 1

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"Mechanistically, USP19 directly interacts with and stabilized NEK9 via inhibiting K48-specific polyubiquitination process on NEK9 protein at K525 site through its USP domain."
NADSYN1 affects HSPH1
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| 1

sparser
"In vitro and in situ interactions of Usp19, DNAAF2, NADSYN1, p23/PTGES3, Hsp105 and Bcl‐G with dcTPR proteins."
NADSYN1 affects AIP
| 1
| 1

sparser
"We obtained moderate to strong labeling for Usp19, DNAAF2, and NADSYN1 interactions with AIP and surprisingly strong labeling of AIP–p23 interactions."
MRPL42 affects USP19
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1 |

No evidence text available
LRPPRC affects USP19
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1 |

No evidence text available
LMBR1L affects USP19
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1 |

No evidence text available
LAT affects USP19
1 |
1 |

No evidence text available
KPTN affects USP19
1 |
1 |

No evidence text available
K63- affects USP19
| 1
USP19 binds K63- and K27. 1 / 1
| 1

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"Mechanistically, USP19 interacted with TAK1 in a TNF-alpha- or IL-1beta-dependent manner and specifically deconjugated K63- and K27 linked polyubiquitin chains from TAK1, leading to the impairment of TAK1 activity and the disruption of the TAK1 and TAB2/3 complex."
K48 affects USP19
| 1
USP19 binds NEK9 and K48. 1 / 1
| 1

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"Mechanistically, USP19 directly interacts with and stabilized NEK9 via inhibiting K48-specific polyubiquitination process on NEK9 protein at K525 site through its USP domain."
K29 affects USP19
| 1
| 1

sparser
"For example, we identified USP32 as a K6 and K29 diUbs selective interactor, while USP19 selectively interacts with K29 diUb."
K27 affects USP19
| 1
USP19 binds K63- and K27. 1 / 1
| 1

reach
"Mechanistically, USP19 interacted with TAK1 in a TNF-alpha- or IL-1beta-dependent manner and specifically deconjugated K63- and K27 linked polyubiquitin chains from TAK1, leading to the impairment of TAK1 activity and the disruption of the TAK1 and TAB2/3 complex."
IARS1 affects USP19
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1 |

No evidence text available
HSPH1 affects USP19
| 1
| 1

sparser
"In vitro and in situ interactions of Usp19, DNAAF2, NADSYN1, p23/PTGES3, Hsp105 and Bcl‐G with dcTPR proteins."
HSPA8 LAMP2A affects USP19
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HSPA8 LAMP2A activates USP19. 1 / 1
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eidos
"Knockdown of HSPA8 or LAMP2A disrupts CMA could restore the TBK1 protein level in the case of USP19 expression ( Zhao et al ., 2021 ) ."
HSPA1A affects USP19
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1 |

No evidence text available
HSP90AB1 affects USP19
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No evidence text available
HNRNPAB affects USP19
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1 |

No evidence text available
HGS affects USP19
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HGS activates USP19. 1 / 1
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"We found that silencing HRS/HGS expression efficiently decreased USP19-mediated TDP-43-K263E secretion indicating the involvement of the late endosomal/amphisome compartments (Fig. 6d)."
HERC2 affects USP19
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1 |

No evidence text available
Gentamicins affects USP19
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Gentamicins increases the amount of USP19. 1 / 1
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No evidence text available
GST affects USP19
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sparser
"BAG6 was subsequently treated with purified GSTUSP19 protein that had been immobilised on protein G beads (Figure  xref )."
GFP-VAMP7-Longin affects USP19
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GFP-VAMP7-Longin inhibits USP19. 1 / 1
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"The data presented in Fig. 7d, e showed that overexpression of the GFP-VAMP7-Longin significantly reduces the USP19-mediated misfolded TDP-43-K263E secretion compared to the control."
GCN1 affects USP19
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1 |

No evidence text available
G3BP1 affects USP19
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1 |

No evidence text available
FLI1 affects Ubiquitin
| 1
| 1

sparser
"As the main ubiquitin acceptor site for EWS-FLI1 degradation is located in the FLI1 terminus xref , we were next interested in identifying whether USP19 still interacts with EWS-FLI1 ubiquitin acceptor site mutants."
FKBP51 affects USP19
| 1
AIP binds FKBP51 and USP19. 1 / 1
| 1

sparser
"We confirmed interaction of ubiquitin carboxypeptidase Usp19 with two dcTPR proteins, AIP and FKBP51, via the dicarboxylate clamp mechanism."
EWSR1 affects Ubiquitin
| 1
| 1

sparser
"As the main ubiquitin acceptor site for EWS-FLI1 degradation is located in the FLI1 terminus xref , we were next interested in identifying whether USP19 still interacts with EWS-FLI1 ubiquitin acceptor site mutants."
ERAD machinery factors affects USP19
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USP19 binds ERAD machinery factors. 1 / 1
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"Intriguingly, although overexpressed USP19 interacts with Derlin-1 and other ERAD machinery factors in the membrane, endogenous USP19 is mostly in the cytosol where it binds Hsp90."
DNAAF2 affects HSPH1, NADSYN1, and USP19
| 1
| 1

sparser
"In vitro and in situ interactions of Usp19, DNAAF2, NADSYN1, p23/PTGES3, Hsp105 and Bcl‐G with dcTPR proteins."
DNAAF2 affects AIP
| 1
| 1

sparser
"We obtained moderate to strong labeling for Usp19, DNAAF2, and NADSYN1 interactions with AIP and surprisingly strong labeling of AIP–p23 interactions."
DET1 affects USP19
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1 |

No evidence text available
DCTN3 affects USP19
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1 |

No evidence text available
CUL4B affects USP19
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1 |

No evidence text available
CUL4A affects USP19
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1 |

No evidence text available
CFTR affects USP19
1 |
1 |

No evidence text available
CDC123 affects USP19
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1 |

No evidence text available
| 1

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"Interestingly, Usp19 also interacts with CaMK-II and since alphakap forms complexes with CaMK-II, it is possible that alphakap may function as a scaffold for Usp19."
CAMK2G affects USP19
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1 |

No evidence text available
C2-FFL affects USP19
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C2-FFL activates USP19. 1 / 1
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"Analysis of the Beclin-1 integral and late balance phase spaces showed that deficiency of C2-FFL severely attenuated hypoxia-mediated inhibition as well as markedly enhanced USP19-dependent protection[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
BIRC5 affects A549
| 1
USP19 binds BIRC5 and A549. 1 / 1
| 1

reach
"Therefore, we first verified the potential effects of gastrodin on the interaction between USP19 and survivin in A549 cells."
B4GALT2 affects USP19
1 |
1 |

No evidence text available

sparser
"We also found that five CsPIN protein members (PIN1, PIN1b, PIN1c, PIN2, and PIN3) in cucumber may interact with ARF and USP19 ( xref )."
Acrylamide affects USP19
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Acrylamide increases the amount of USP19. 1 / 1
1 |

No evidence text available
AZM551248 affects USP19
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AZM551248 increases the amount of USP19. 1 / 1
1 |

No evidence text available
ATM affects USP19
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ATM phosphorylates USP19. 1 / 1
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"In addition, USP19 is phosphorylated by ATM or ATR in response to DNA damage [14]."
ARL8B affects USP19
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1 |

No evidence text available
APEX1 affects USP19
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1 |

No evidence text available
AIP affects NADSYN1
| 1
| 1

sparser
"We obtained moderate to strong labeling for Usp19, DNAAF2, and NADSYN1 interactions with AIP and surprisingly strong labeling of AIP–p23 interactions."
AIP affects FKBP51, and USP19
| 1
AIP binds FKBP51 and USP19. 1 / 1
| 1

sparser
"We confirmed interaction of ubiquitin carboxypeptidase Usp19 with two dcTPR proteins, AIP and FKBP51, via the dicarboxylate clamp mechanism."
AIP affects DNAAF2, NADSYN1, and USP19
| 1
| 1

sparser
"We obtained moderate to strong labeling for Usp19, DNAAF2, and NADSYN1 interactions with AIP and surprisingly strong labeling of AIP–p23 interactions."
ACSM3 affects USP19
| 1
ACSM3 inhibits USP19. 1 / 1
| 1

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"Additionally, low-dose LPS counteracted SAH-decreased USP19, FOXO1, IL-10 and IL-10R1, along with SAH-increased MMP9 in mouse brain (Fig. 1H)."
A549 affects USP19
| 1
USP19 binds BIRC5 and A549. 1 / 1
| 1

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"Therefore, we first verified the potential effects of gastrodin on the interaction between USP19 and survivin in A549 cells."
5a affects USP19
| 1
5a activates USP19. 1 / 1
| 1

reach
"By further integrating interaction proteomics with transcriptomics and network analysis it was found that NS5A from hepatitis C virus (HCV) modulates the host cell proliferation properties by inhibiti[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
2,4-D affects USP19
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2,4-D increases the amount of USP19. 1 / 1
1 |

No evidence text available

No evidence text available
4,4'-sulfonyldiphenol decreases the amount of USP19. 1 / 1
1 |

No evidence text available