IndraLab

Statements


USP13 affects PTEN
8 1 | 34 12
USP13 binds PTEN.
8 | 8 11
8 | 8 10

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"Considering that loss of PTEN contributed to fibroblasts phenotypic changes and USP13 interacted with PTEN and functioned as a deubiquitylase in epithelial cells, we subsequently sought to determine whether USP13 deficiency is responsible for the low PTEN expression observed in IPF fibroblasts."

sparser
"Moreover, purified GST-USP13, but not the GST-GFP control, was able to bind to FLAG-tagged PTEN under cell-free conditions ( xref ), which demonstrated a direct interaction between USP13 and PTEN."

sparser
"In human breast cancer, loss of USP13 is highly associated with loss of PTEN."

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"Interestingly, we found that USP13 interacted with PTEN in fibroblasts and regulated PTEN ubiquitination and degradation, supporting the idea that IPF is a cancer like disease."

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"We sought to determine whether USP13 directly interacts with PTEN and functions as a bona fide PTEN deubiquitinase."

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"Mechanistically, USP13 directly interacted with PTEN."

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"To further validate the interaction between USP13 and PTEN, an immunofluorescent confocal microscopic analysis was conducted."

sparser
"Additionally, USP13 interacted with the tumor suppressor phosphatase and tensin homolog (PTEN)."

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"Recently, a new study found that USP13 interacted with PTEN in fibroblasts and regulated PTEN ubiquitination and degradation."

sparser
"Co-immunoprecipitation assay was performed to assess the interaction between USP13 and PTEN protein."
USP13 binds OTUD3 and PTEN. 1 / 1
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sparser
"USP7, a highly expressed DUB in prostate cancer and progressive multifocal leukoencephalopathy (PML), plays a direct role in PTEN deubiquitination and regulates its localization rather than protein stability. xref In addition, USP13 and OTUD3 can interact with PTEN and remove its polyubiquitin chain."
USP13 increases the amount of PTEN.
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USP13 increases the amount of PTEN. 7 / 7
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"Furthermore, restoration of USP13 rescued PTEN expression, cell phenotypes and xenograft tumor growth which is altered by NF-kB activation."

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"In addition, reintroduction of USP13 partially rescued PTEN expression as well as the oncogenesis trend caused by NF-kB."

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"Results indicated that USP13 knockdown notably decreased PTEN protein expression and increased phosphor-AKT levels in BC cells."

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"Although each of these five DUBs could interact with endogenous PTEN (XREF_SUPPLEMENTARY), only one of them, USP13, significantly increased endogenous PTEN protein expression (XREF_SUPPLEMENTARY)."

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"Moreover, reintroduction of USP13 repressed the signaling transduction of NF-kB and largely rescued PTEN expression in NF-kB activated or miR-130b/301b overexpressed BC cells."

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"Results suggested that the overexpression of NF-kB p65 significantly decreased the expression levels of USP13 and PTEN, and re-introduction of USP13 rescued PTEN expression."

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"Our data revealed that knockdown of USP13 by short hairpin RNA (shRNA) significantly decreased PTEN protein expression in BC cells."
Modified USP13 increases the amount of PTEN. 2 / 2
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"Collectively, these data suggest that loss of USP13 may contribute to loss of PTEN in a substantial fraction of human tumors, whereas in other tumors PTEN can be inactivated by different mechanisms, including genetic alterations and upregulation of PTEN ubiquitin ligases (such as NEDD4-1 14 and WWP2 15)."

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"Additionally, we noticed that in fibroblasts, the loss of USP13 down-regulated PTEN protein expression by increasing PTEN ubiquitination."
USP13 deubiquitinates PTEN.
1 | 7
USP13 deubiquitinates PTEN. 8 / 8
1 | 7

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"Therefore, USP13 can directly deubiquitinate PTEN."

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"Wild-type USP13 purified from either bacteria or 293T cells, but not its catalytically inactive mutant C345A, decreased PTEN poly-ubiquitination by 64-70% in vitro (XREF_FIG)."

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"USP13 deubiquitinates and stabilizes PTEN."

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"Deubiquitination and stabilization of PTEN by USP13."

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"Deubiquitination of PTEN by USP13, a deubiquitinating enzyme, stabilized PTEN, and thereby inhibited breast cancer tumorigenesis [5]."

"Deubiquitylation and stabilization of PTEN by USP13"

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"Deubiquitylation and stabilization of PTEN by USP13."

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"On the other hand, ectopic expression of wild-type USP13, but not the C345A mutant which is still capable of interacting with PTEN (XREF_FIG), reduced the poly-ubiquitination of PTEN by 65% (XREF_FIG), suggesting that the enzymatic activity of USP13 is indispensable for USP13 dependent deubiquitination of PTEN."
USP13 decreases the amount of PTEN.
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USP13 decreases the amount of PTEN. 3 / 4
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"Two independent USP13 shRNAs both decreased PTEN protein expression by 80% and increased phospho-AKT and phospho-FOXO1/3 levels by 3- to 5-fold in SUM159 breast cancer cells, while restoration of PTEN or expression of an RNAi resistant ' silence mutant ' (i.e., no amino acid change) of USP13 (USP13-RE) in USP13 depleted SUM159 cells completely reversed the effect of USP13 shRNA on upregulating the phosphorylation of AKT and FOXO (XREF_FIG and XREF_SUPPLEMENTARY)."

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"Reintroduction of USP13 could block the pathway and rescue PTEN protein expression, and thereby inhibited the carcinogenesis of bladder cells."

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"Knockdown of USP13 by USP13 shRNA, miR-130b/301b overexpression or NF-kB activation in BC cells leads to the loss of PTEN expression, which was subsequently demonstrated to be reversed by reintroduction of USP13."
Modified USP13 decreases the amount of PTEN. 2 / 2
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"Furthermore, USP13 overexpression induced phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression and repressed the activation of AKT as well as the expression of the downstream effectors glucose transporter-1 (GLUT1) and hexokinase-2 (HK2)."

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"Overexpression of miR-130b/301b or USP13 knockdown by shRNA delivery decreases USP13 expression, and further reduces PTEN protein expression and leads to enhancement of cell proliferation, invasion and migration."
USP13 activates PTEN.
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USP13 activates PTEN. 2 / 6
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"These data supported our notion that USP13 suppresses tumorigenesis by blocking the NF-kB-driven PTEN downregulation in BC."

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"USP13 mediates PTEN to ameliorate osteoarthritis by restraining oxidative stress, apoptosis and inflammation via AKT dependent manner."
USP13 inhibits PTEN.
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USP13 inhibits PTEN. 2 / 4
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"Conversely, overexpression of USP13 suppresses tumorigenesis and glycolysis in PTEN positive but not PTEN-null breast cancer cells."

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"Down-regulation of USP13 mediates PTEN protein loss and fibroblast phenotypic change, and thereby plays a crucial role in IPF pathogenesis."
USP13 ubiquitinates PTEN.
| 1 1
Modified USP13 leads to the ubiquitination of PTEN. 1 / 1
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"Indeed, silencing USP13 expression by two independent shRNAs increased PTEN poly-ubiquitination by approximately 3-fold (XREF_FIG)."
USP13 ubiquitinates PTEN. 1 / 1
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sparser
"In order to determine whether PTEN is a direct substrate of USP13, we purified USP13 and ubiquitinated PTEN and then incubated them in a cell-free system."
USP13 affects MCL1
1 | 45 8
USP13 binds MCL1.
| 11 8
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"Therefore, we propose that USP13 is a bona fide deubiquitinase for MCL1, a notion that is further supported by the endogenous interaction and correlative expression of USP13 and MCL1 only at protein levels in clinical tumor samples."

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"Moreover, the interaction of USP13 and MCL1 occurred between endogenous proteins in A549, HEY and SW-1573 tumor cells."

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"We further conducted the in vitro immunoprecipitation experiment, the result of which suggested that the interaction between USP13 and MCL1 was direct and independent on MCL1 ubiquitination status."

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"USP13 interacts with and deubiquitinates MCL1."

sparser
"In vitro assays revealed the interaction of USP13 and MCL-1 and USP13 could deubiquitinate and stabilize MCL-1 ( xref ; xref )."

sparser
"We further conducted the in vitro immunoprecipitation experiment, the result of which suggested that the interaction between USP13 and MCL1 was direct and independent on MCL1 ubiquitination status (Fig.  xref )."

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"USP13 interacts with and deubiquitinates Mcl-1 in cervical cancer cells."

sparser
"Second, USP13 interacted with MCL1 and decreased MCL1 polyubiquitination in a DUB activity-dependent manner."

sparser
"However, correlation analysis of RNA sequencing data from TCGA failed to provide evidence that USP13 and MCL1 were associated with each other at the messenger RNA (mRNA) level (Supplementary Fig.  xref ), implying that USP13-mediated regulation of MCL1, if any, might not occur through a transcriptional mechanism."

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"To gain insights into USP13 mediated regulation of MCL1, we tested whether USP13 interacted with MCL1 and functioned as a deubiquitinase."
USP13 binds MCL1 and FlaG. 1 / 1
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sparser
"Immunoprecipitation with anti-FLAG antibody demonstrated that FLAG-USP13 interacted with over-expressed Mcl-1 (Fig. xref )."
USP13 activates MCL1.
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USP13 activates MCL1. 10 / 16
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"Taken together, our results indicated that pharmacological inhibition of USP13 by spautin-1 reduced MCL1 protein abundance and increased tumor cell sensitivity to ABT-263."

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"The deubiquitinase (DUB) USP13 promotes Mcl-1 stabilisation in cervical cancer."

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"We subsequently investigated the impact of USP13 mediated MCL1 stabilization on tumor malignancy."

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"Conversely, overexpression of WT USP13, but not the USP13 mutant, enhanced the half-life of Mcl-1 to ~ 300min."

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"Our data shows that USP13 and Mcl-1 protein expression correlates, suggesting that USP13 mediated Mcl-1 stabilisation is clinically relevant."

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"As a complimentary approach, we lentivirally expressed ectopic USP13 into OVCAR5, PC9 and NCI-H2170 cells, whose endogenous USP13 levels were relatively low, and found that overexpression of USP13 increased MCL1 protein, but not mRNA, abundance."

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"These data demonstrate that USP13 promotes the stability of Mcl-1 in HPV+ cervical cancer cells by protecting it from proteasomal degradation."

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"First, USP13 promoted MCL1 protein stability in multiple lung and ovarian cancer cell models."

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"In lung infection, USP13 are aberrantly expressed, inhibition of USP13 reduces the abundance of anti-apoptotic protein MCL1 in the lung [42]."

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"USP13 promotes MCL1 protein stability."
USP13 increases the amount of MCL1.
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USP13 increases the amount of MCL1. 7 / 7
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"Interestingly, Zhang et al. showed that pharmacological inhibition of USP13 by a small-molecule inhibitor, spautin-1, markedly downregulated MCL1 protein levels and sensitized ovarian and lung cancer cells to ABT-263, a selective antagonist of BCL-2 and BCL-XL.We selected DUB3 as an ideal target in ovarian cancer due to its oncogenic functions in various cancer subtypes ."

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"We confirmed the siRNA screen results by transfecting each of the four oligos against USP13 into HEK293T cells, and indeed found that USP13 knockdown decreased the endogenous protein levels of MCL1, while modulating USP9X or OTUB2 with different siRNAs had no consistent effects on MCL1 expression."

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"Interestingly, over-expression or depletion of USP13 did not modulate Mcl-1 expression in HPV- C33A cells, suggesting that Mcl-1 expression is not regulated by USP13 in these cells."

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"In line with these observations, MCL1 protein levels were downregulated by depletion of USP9X but not USP13 (Figure S6D)."

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"Critically, treatment of USP13 depleted cells with the proteasome inhibitor MG132 rescued Mcl-1 protein levels, suggesting that USP13 protects Mcl-1 from proteasomal degradation."

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"Loss of USP13 significantly reduced Mcl-1 protein expression, without effecting MCL1 mRNA expression."

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"In addition, we explored whether pharmacological inhibition of USP13 downregulated MCL1 protein expression and synergistically kill tumor cells in combination with ABT-263."
USP13 deubiquitinates MCL1.
1 | 5
USP13 deubiquitinates MCL1. 6 / 6
1 | 5

"Collectively, we nominate USP13 as a novel deubiquitinase which regulates MCL1 turnover in diverse solid tumors and propose that USP13 may be a potential therapeutic target for the treatment of various malignancies."

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"Two DUBs USP9X and USP13 deubiquitinate and stabilise MCL1, and hypomorphic mutations in both have been linked to neurodevelopmental disorders and neurodegenerative disease [XREF_BIBR, XREF_BIBR]."

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"Next, we confirmed that USP13 deubiquitinated Mcl-1."

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"Therefore, USP13 interacted with and deubiquitinated MCL1 to preserve its protein stability."

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"USP13 interacts with and deubiquitinates Mcl-1 in cervical cancer cells."

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"USP13 interacts with and deubiquitinates MCL1."
USP13 decreases the amount of MCL1.
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USP13 decreases the amount of MCL1. 2 / 2
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"In addition, genetic depletion of USP13 using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9, or pharmacological inhibition of USP13 by a small-molecule inhibitor spautin-1, markedly downregulates MCL1 protein expression and shows synergistic effects against tumor cells in combination with ABT-263, a selective antagonist of BCL-2 and BCL-XL."

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"Interestingly, although USP13 depletion reduced MCL1 protein levels, neither cell proliferation nor cell migration was significantly changed under normal growth conditions."
USP13-C345A decreases the amount of ubiquitinated MCL1. 1 / 1
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"Consistent with USP13 removing ubiquitin from MCL1, wild type USP13, but not catalytically inactive mutant USP13 C345A, markedly reduced the amount of ubiquitinated MCL1."
USP13 ubiquitinates MCL1.
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USP13 leads to the ubiquitination of MCL1. 2 / 2
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"Conversely, ubiquitination of Mcl1 can be reversed by the deubiquitinase USP9X and the more recently identified USP13 ."

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"Finally, we confirmed that depletion of USP13 from both HeLa and SiHa cells increased the ubiquitination of endogenous Mcl-1, suggesting USP13 mediated deubiquitination of Mcl-1 is relevant in cervical cancer cells."
USP13 inhibits MCL1.
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USP13 inhibits MCL1. 1 / 1
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"Hence, pharmaceutical intervention of USP13 activity is expected to antagonize the tumorigenic potential of MCL1 oncoprotein, and combined administration of USP13 inhibitors with clinically approved venetoclax therapy may represent a promising targeting strategy for the treatment of human cancer by inducing tumor cell death."
USP13 affects MYC
1 | 29 8
USP13 binds MYC.
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| 6 4

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"To investigate whether USP13 mediated the deubiquitination of cohesin subunits, we cotransfected 293T cells with expression vectors for His-ubiquitin and Myc-USP13, purified ubiquitinated proteins by Ni-NTA affinity chromatography, and performed Western blot with antibodies to SMC3 and RAD21 ( xref A )."

sparser
"Computational modeling of structures predicted in ZDOCK and Pymol software showed that Y708 phosphorylation was necessary for the direct binding of USP13 to c-Myc ( xref )."

sparser
"We then examined the physiological significance of USP13 phosphorylation by CLK3 at Y708. xref indicates that CLK3 knockdown in HuCCT1 cells strongly impaired the interaction of USP13 with c-Myc."

sparser
"As USP13 is a deubiquitinase, the interaction between USP13 and c-Myc led us to hypothesize that USP13 may stabilize c-Myc protein in GSCs through deubiquitination."

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"These results demonstrate that c-Myc interacts with USP13 and FBXL14 in glioma cells, indicating that deubiquitinase USP13 and the ubiquitin E3 ligase FBXL14 are potential regulators of c-Myc protein stability in GBM."

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"However, overexpression of CLK3 in HCCC9810 cells promoted the binding of c-Myc to WT-USP13, not USP13 Y708F mutant (XREF_FIG)."

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"USP13 and FBXL14 interact with c-Myc in glioma cells."

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"The interaction between c-Myc and USP13 or FBXL14 was validated in HEK293 cells coexpressing c-Myc and Flag tagged USP13 or FBXL14."

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"Computational modeling of structures predicted in ZDOCK and Pymol software showed that Y708 phosphorylation was necessary for the direct binding of USP13 to c-Myc (XREF_FIG)."

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"As USP13 is a deubiquitinase, the interaction between USP13 and c-Myc led us to hypothesize that USP13 may stabilize c-Myc protein in GSCs through deubiquitination."
USP13 binds FBXL14 and MYC. 4 / 4
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sparser
"These results demonstrate that c-Myc interacts with USP13 and FBXL14 in glioma cells, indicating that deubiquitinase USP13 and the ubiquitin E3 ligase FBXL14 are potential regulators of c-Myc protein stability in GBM."

sparser
"The interaction between c-Myc and USP13 or FBXL14 was validated in HEK293 cells coexpressing c-Myc and Flag-tagged USP13 or FBXL14."

sparser
"To further confirm the interaction between endogenous c-Myc and USP13 or FBXL14 in GSCs, c-Myc was immunoprecipitated from cell lysate of partially differentiated GSCs that were induced by serum acutely (2 d)."

sparser
"USP13 and FBXL14 interact with c-Myc in glioma cells."
USP13 activates MYC.
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USP13 activates MYC. 2 / 8
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"Collectively, these data demonstrate that the ubiquitination insensitive mutant of c-Myc is able to rescue the phenotypes caused by USP13 knockdown or FBXL14 overexpression in vitro and in vivo, which supports that USP13 and FBXL14 mediate posttranslational regulation of c-Myc to control the stem cell like phenotype and tumorigenic potential of GSCs."

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"We found a positive correlation between TRF1 levels and all the stem cell markers with the exception of MYC, although TRF1 was positively correlated with the MYC modulator USP13 (Fang et al., 2017)."
USP13 activates MYC. 2 / 2
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"In addition, IHC staining of Ki-67 and cleaved caspase 3 confirmed that USP13 disruption reduced c-Myc protein in the GSC derived xenografts and led to a significant decrease in cell proliferation and an increase in cell apoptosis within the tumor (not depicted)."

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"CHX chase assays indicated that overexpression of WT-USP13, particularly a phosphomimetic USP13-Y708E, but not USP-Y708F, significantly up-regulated the half-time of the c-Myc protein in HEK293 cells (XREF_FIG)."
USP13-Y708F activates MYC. 1 / 1
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"The expression of USP13-Y708E but not USP13 Y708F mutant significantly promoted the recruitment of c-Myc on the promoters of purine associated enzymes (XREF_FIG), consequently enhancing their expression (XREF_FIG)."
USP13 increases the amount of MYC.
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USP13 increases the amount of MYC. 5 / 5
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"Targeting USP13 reduces c-Myc protein levels and impairs GSC maintenance."

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"As USP13 functions as a deubiquitinase that also interacts with c-Myc (XREF_FIG) and the preferential expression of USP13 in GSCs positively regulates c-Myc protein levels (XREF_FIG), we hypothesized that USP13 might mediate deubiquitination of c-Myc protein to prevent its degradation and stabilize c-Myc in GSCs."

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"In this study, we demonstrated that USP13 mediated deubiquitination and FBXL14 mediated ubiquitination regulate c-Myc protein levels in glioma cells and play a critical role in controlling the maintenance or differentiation of GSCs (XREF_FIG)."

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"In vitro analysis showed inducible disruption of USP13 in GSCs by 70% reduced c-Myc protein levels and inhibited GSC proliferation (XREF_FIG)."

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"The ubiquitination assay demonstrated that disruption of USP13 by shRNA markedly increased c-Myc ubiquitination and reduced c-Myc protein level in GSCs (XREF_FIG)."
USP13 increases the amount of MYC. 2 / 2
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"In HCC cell lines, knockdown of USP13 by shRNAs markedly decreased HCC cell growth, and mechanistic investigations revealed that USP13 knockdown could markedly downregulate the expression levels of c-Myc."

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"The knockdown of USP13 by short hairpin RNAs (shRNAs) markedly decreases cell growth in HCC by reducing c-Myc expression."
Modified USP13 increases the amount of MYC. 1 / 1
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"In contrast, overexpression of USP13 (Flag-USP13) increased c-Myc protein levels in GSCs (XREF_FIG)."
USP13 deubiquitinates MYC.
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USP13 deubiquitinates MYC. 5 / 5
1 | 4

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"Our data have demonstrated that USP13 mediates deubiquitination of c-Myc, whereas FBXL14 facilitates c-Myc ubiquitination in glioma cells."

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"Moreover, USP13 knockdown increased c-Myc ubiquitination, whereas FBXL14 knockdown reduced c-Myc ubiquitination (XREF_FIG)."

"In this study, we demonstrate that the deubiquitinase USP13 stabilizes c-Myc by antagonizing FBXL14-mediated ubiquitination to maintain GSC self-renewal and tumorigenic potential."

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"The ubiquitination assay demonstrated that disruption of USP13 by shRNA markedly increased c-Myc ubiquitination and reduced c-Myc protein level in GSCs (XREF_FIG)."

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"Furthermore, USP13 can deubiquitinate and stabilise ACLY and OGDH and c-Myc in ovarian cancer and glioblastoma, respectively, thereby functioning as an oncogene [XREF_BIBR, XREF_BIBR]."
Modified USP13 leads to the deubiquitination of MYC. 2 / 2
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"Importantly, overexpression of USP13 together with FBXL14 abolished the increased c-Myc ubiquitination caused by FBXL14 overexpression (XREF_FIG, last lane)."

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"Overexpression of USP13 reduced c-Myc ubiquitination, and forced expression of FBXL14 increased c-Myc ubiquitination (XREF_FIG)."
USP13 leads to the deubiquitination of MYC. 1 / 1
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"As USP13 functions as a deubiquitinase that also interacts with c-Myc (XREF_FIG) and the preferential expression of USP13 in GSCs positively regulates c-Myc protein levels (XREF_FIG), we hypothesized that USP13 might mediate deubiquitination of c-Myc protein to prevent its degradation and stabilize c-Myc in GSCs."
USP13 ubiquitinates MYC.
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USP13 leads to the ubiquitination of MYC. 2 / 2
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"Silencing FBXL14 alongside USP13 disruption attenuated the increased c-Myc ubiquitination caused by USP13 disruption (XREF_FIG)."

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"USP13 mediates ubiquitination of c-Myc to antagonize FBXL14 mediated ubiquitination and thus maintains the stem cell like phenotype and tumorigenic potential of GSCs."
USP13 inhibits MYC.
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USP13 inhibits MYC. 1 / 2
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"Moreover, treatment with a proteasome inhibitor blocked the c-Myc loss caused by USP13 knockdown (not depicted), but overexpression of USP13 delayed c-Myc turnover in the presence of cycloheximide (not depicted), suggesting a critical role of USP13 in preventing proteasomal degradation of c-Myc in GSCs."
USP13 affects BECN1
1 | 26 13
USP13 binds BECN1.
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| 7 11

sparser
"Intriguingly, a previous study revealed that USP13 and Beclin1 could interact with each other."

sparser
"Furthermore, Beclin1 also interacts with USP13 and regulates the stability of USP13."

sparser
"Since ΔC-Beclin1 can bind to Vps34 ( xref ) but not USP13, this experiment suggests that the interaction of Beclin1 and USP13 is critically important for regulating the stability of Vps34 complexes in response to spautin-1 treatment."

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"Since the interaction of USP13 and Beclin1 is detectable by coimmunoprecipitation, we propose that Beclin1 may have close interaction with USP13."

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"Since the interaction of USP13 and Beclin1 is detectable by coimmunoprecipitation, we propose that Beclin1 may have close interaction with USP13."

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"We found that endogenous Beclin1 can interact with USP13 and the interaction was reduced in the presence of spautin-1 ( xref )."

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"Because a direct interaction of USP13 with Beclin 1 has been detected, it is thought that USP13 counteracts the ubiquitination of Beclin 1 by yet unknown ligases [ xref ]."

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"These data suggest that Beclin1 may closely interact with USP13, whereas its interaction with USP10 is indirect or transient in nature."

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"These data suggest that Beclin1 may closely interact with USP13, whereas its interaction with USP10 is indirect or transient in nature."

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"Since DeltaC-Beclin1 can bind to Vps34 but not USP13, this experiment suggests that the interaction of Beclin1 and USP13 is critically important for regulating the stability of Vps34 complexes in response to spautin-1 treatment."
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sparser
"To determine the mechanism by which Vps34 complexes regulate the stability of USP13 and USP10, we examined the possibility that Beclin1 may interact with USP10 and USP13."

sparser
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."
USP13 deubiquitinates BECN1.
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USP13 deubiquitinates BECN1. 9 / 9
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"Recent studies indicated that USP13 can deubiquitinate and regulate protein levels of Beclin-1, microphathalmia associated transcription factor, Siah2, phosphatase and tensin homolog, and STAT-1, and the deubiquitination process of USP13 could also be orchestrated by Beclin-1."

"While A20 inhibits PtdIns3P signaling by removing the TRAF6-dependent Lys63-linked chains from Beclin 1, the enzymes USP10 and USP13 prevent PI3K-III complex components from their degradation and, therefore, support autophagy. Interestingly enough, USP10 also stabilizes p53, which, in turn, triggers the degradation of Beclin 1 and VPS34 in order to prevent autophagy."

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"USP10 and USP13 have been shown to mediate the deubiquitination of Beclin 1, thereby stabilizing the Vps34 complex XREF_BIBR."

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"Furthermore, the presence of spautin-1 inhibited the deubiquitination of Beclin1 mediated by USP10 and USP13."

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"We demonstrate that USP10 and USP13 can both mediate the deubiquitination of Beclin1."

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"It promotes the degradation of Vps34 complexes by inhibiting USP10 and USP13, two ubiquitin specific peptidases that target the deubiquitination of Beclin-1."

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"Likewise, USP13, which deubiquitinates BECN1 and stabilizes PIK3C3 complexes, is amplified in LUSQ."

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"Conversely, it was show that Beclin 1 is deubiquitinated by USP10 and USP13 and adding complexity, Beclin 1 itself controlled the protein stabilities of USP10 and USP13 by regulating their deubiquitinating activities, in turn regulating the levels of tumor suppressor p53 [XREF_BIBR]."

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"Specific and potent autophagy inhibitor-1 (Spautin-1) was identified to inhibit USP10 and USP13, which deubiquitinate the Beclin 1 subunit of Vsp34 complex, and thus promoted the degradation of Vsp34 PI3 kinase complex."
USP13 activates BECN1.
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USP13 activates BECN1. 6 / 6
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"Overexpression of USP13 in PC12 cells almost reversed the Spautin‐1‐mediated decrease in NLRP3, USP13, Beclin1 and GSDMD‐N expression levels (Figure 3D,E)."

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"XREF_BIBR Spautin-1 promotes the degradation of BECN1 by inhibiting 2 USPs (ubiquitin specific peptidases), USP10 and USP13, which target BECN1."

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"Spautin-1, a potent small molecule, can promote the degradation of PI3KCIII and Vps34 complexes by inhibiting two ubiquitin specific peptidases, USP10 and USP13 which target the Beclin-1 subunit of Vp[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Spautin-1 has been recently reported to prevent autophagy by inhibiting the deubiquitinases USP10 and USP13, which leads to beclin 1 and Vps34 (or PI3KIII) degradation [XREF_BIBR]."

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"This molecule promotes the degradation of Vps34-PI 3 kinase complexes by inhibiting two ubiquitin specific peptidases, USP10 and USP13, that target the Beclin-1 subunit of Vps34 complexes."

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"The function of USP10 and USP13 is reversed by a chemical compound, spautin-1, which inhibits autophagy by promoting Beclin-1 degradation."
USP13 ubiquitinates BECN1.
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USP13 leads to the ubiquitination of BECN1. 4 / 4
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"Chemical Inhibition of the Deubiquitinating Enzymes USP10 and USP13 Causes Ubiquitination and Degradation of BECLIN 1."

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"On the other hand, autophagy can be also inhibited blocking PI3KC3 complex formation; Spautin-1 indirectly inhibits the activity of VPS34 by proteosomal degradation of proteins that form VPS34 complexes through reduction of Beclin-1 deubiquitination mediated by USP10 and USP13 [120]."

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"For instance, USP10 and USP13 reduce Beclin-1 ubiquitination to prevent its degradation."

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"Inhibition of the Deubiquitinating Enzymes USP10 and USP13 Causes Ubiquitination of Beclin 1."
USP13 increases the amount of BECN1.
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USP13 increases the amount of BECN1. 1 / 1
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"Moreover, beclin-1 and USP10 are involved in a potential feedforward mechanism in which beclin-1 stabilizes USP13, which in turn deubiquitinates and stabilizes USP10, leading to increased beclin-1 levels and activity."
USP13 is modified
3 | 34
USP13 is phosphorylated.
3 | 27
USP13 is phosphorylated on Y708. 10 / 10
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sparser
"Notably, the CCA-associated CLK3-Q607R mutant induced USP13-Y708 phosphorylation and enhanced the activity of c-Myc."

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"We made a special antibody against USP13-Y708 phosphorylation for the following experiments."

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"As expected, USP13-Y708E transfection in HEK293 cells significantly inhibited the binding of c-Myc to Fbxl14, while USP13-Y708F significantly increased it (data not shown), revealing that USP13 phosphorylation at Y708 reverts c-Myc ubiquitination mediated by Fbxl14, thereby enhancing its stability ( xref )."

sparser
"Further analysis found that tacrine hydrochloride significantly decreased CLK3-Q607R–enhanced purine production and USP13-Y708 phosphorylation in CCA cells ( xref )."

sparser
"CLK3 mediated the phosphorylation of USP13 at Y708, promoting its binding to c-Myc, which is an important transcription factor and also known as an oncogene in many cancers [71] ."

sparser
"Thus, CLK3-mediated phosphorylation of USP13 at Tyr708 promotes cholangiocarcinoma progression by activating c-Myc-induced purine synthesis, providing a new and viable therapeutic target for the treatment of cholangiocarcinoma associated with CLK3 mutations ( xref )."

sparser
"Furthermore, CLK3-induced phosphorylation of the Tyr708 residue in USP13 promotes cholangiocarcinoma progression by activating c-Myc-mediated purine synthesis ( xref )."

sparser
"The Q607R mutant also up-regulated USP13 phosphorylation at Y708 ( xref Bii)."

sparser
"Similar data were also observed in the EGF-induced increase in CLK3 expression, USP13 phosphorylation at Y708, and proliferation in CCA cells ( xref )."

sparser
"We demonstrated that (1) a recurrent Q607R somatic substitution in CLK3 was identified in 8% of 100 human CCAs, particularly in patients with CCA metastasis; (2) the expression of CLK3 was significantly up-regulated in CCA compared with matched control tissues; (3) CLK3 knockdown significantly inhibited CCA aggressiveness in vitro and in vivo; (4) gene ontology term enrichment and MS assays indicated that high CLK3 expression in CCA patients mainly regulated nucleotide metabolism, especially purine biosynthesis; (5) mechanistically, CLK3 directly phosphorylated USP13 at Y708, which promoted its binding to c-Myc, a critical purine synthesis–associated transcription factor, thereby preventing Fbxl14-mediated c-Myc ubiquitination and activating the transcription of purine metabolic genes; (6) the CCA-associated CLK3-Q607R mutant induced USP13-Y708 phosphorylation and enhanced the activity of c-Myc; (7) in turn, c-Myc transcriptionally up-regulated CLK3; (8) importantly, levels of CLK3 significantly correlated with the expression of phospho-USP13-Y708, c-Myc, and ATIC in human CCA specimens; and (9) tacrine hydrochloride was identified as a potential compound to inhibit the aberrant CLK3-enhanced CCA invasiveness."
USP13 is phosphorylated. 10 / 10
| 10

sparser
"Next, we investigated how USP13 phosphorylation regulates its localization."

sparser
"A recent study suggested that DNA damage could induce the phosphorylation of USP13 by the ATM and then activated USP13 deubiquitinated RAP80 (K75, K90, and K112)."

sparser
"We found that following cisplatin treatment, USP13 is phosphorylated at SQ/TQ motifs ( xref and xref ), which are consensus ATM or ATR phosphorylation sites xref ."

sparser
"We then examined the physiological significance of USP13 phosphorylation by CLK3 at Y708. xref indicates that CLK3 knockdown in HuCCT1 cells strongly impaired the interaction of USP13 with c-Myc."

sparser
"As shown in xref , T196 was phosphorylated after DNA damage, and the T196A mutation inhibited USP13 phosphorylation after DNA damage."

sparser
"Taken together, these results demonstrate that USP13 phosphorylation by ATM is important for USP13 recruitment to DSB sites following DNA damage and proper DDR."

sparser
"Together, these results suggest that USP13 phosphorylation by ATM is important for its recruitment to DSBs, DDR and chemo sensitivity."

sparser
"Here the authors report that phosphorylated USP13 deubiquitinates RAP80 after DNA damage, prompting recruitment to the break site."

sparser
"To further confirm these results, we generated site-specific antibody against p-Thr196 and examined the USP13 phosphorylation following DNA damage."

sparser
"An in vitro kinase assay indicated that WT-CLK3 but not CLK3-K186M was able to directly phosphorylate USP13 ( xref )."
USP13 is phosphorylated on T196. 4 / 4
| 4

sparser
"Recently, researchers from Mayo Clinic uncover that ATM is activated in response to IR damage, and then phosphorylates USP13 at Thr196 site to stabilize RAP80, leading to the efficient recruitment of BRCA1 to the damage sites xref ."

sparser
"To further demonstrate the direct interaction between the MDC1 FHA domain and phosphorylated Thr 196 of USP13, T196 or p-T196 peptide was incubated with purified GST-MDC1 FHA domain in vitro ."

sparser
"Recently, some scientists have reported that ATM is activated to phosphorylate USP13 at Thr196 site in response to DNA damage, stabilizing RAP80 to assemble BRCA1 to repair DSBs xref ."

sparser
"Moreover, ATM-induced phosphorylation of the Thr196 residue in USP13 after DNA damage functions as an essential regulatory event, and plays a critical role in the resistance of cancer cells to chemotherapy by deubiquitinating RAP80, promoting recruitment of complexes, and eliciting a DDR ( xref ; xref ) ( xref )."
USP13 is phosphorylated on T122. 3 / 3
2 | 1

No evidence text available

No evidence text available

sparser
"Here, we found that USP13 is phosphorylated at Thr122 in ovarian cancer cells."
USP13 is phosphorylated on S114. 2 / 2
| 2

sparser
"Studies have shown that Aurora B phosphorylates USP13 at Ser114 and promotes its interaction with USP13."

sparser
"Additionally, kinase Aurora B induced phosphorylation of the Ser114 residue in USP13 prevents several major human cancers by promoting its interaction with partners and stability ( xref )."
USP13 is phosphorylated on S228. 1 / 1
1 |

No evidence text available
USP13 is ubiquitinated.
| 7
USP13 is ubiquitinated. 7 / 7
| 7

sparser
"USP13 and gp78 control ubiquitination of Ubl4A."

sparser
"From the time points of LPS’ effect, we hypothesized that activation of JNK by LPS does not directly affect USP13 ubiquitination."

sparser
"To investigate the molecular regulation of the unknown protein, which regulates USP13 ubiquitination and degradation, we examined several factors related to transcriptional regulation."

sparser
"LPS-induced USP13 degradation was attenuated by actinomycin D ( xref ), suggesting a new protein induced by LPS regulates USP13 ubiquitination."

sparser
"On the other hand, coexpression of USP10 with USP13 has a much less pronounced effect on ubiquitination of USP13 (data not shown)."

sparser
"To investigate whether LPS regulates USP13 degradation through ubiquitination, we examined the effect of LPS on USP13 ubiquitination."

sparser
"We found that short time exposure of LPS did not trigger USP13 degradation, suggesting that an immediate LPS signaling through TLR4 does not contribute to USP13 ubiquitination and degradation."
| 3 27
| 3 14

reach
"This data suggests that LPS induces synthesis of a USP13 degrading protein through activation of JNK in Kupffer cells."

reach
"The effect of JNK inhibitor II on LPS-induced USP13 degradation was confirmed by an experiment using increasing doses of the inhibitor (Fig. 7E)."

reach
"Catalytic activity of USP13 is not necessary for LPS-reduced USP13.."

reach
"As shown above, LPS induced USP13 degradation in 24 h, but not in a shorter time exposure (4 and 8 h)."

reach
"MG-132, but not leupeptin, attenuated LPS-induced USP13 degradation (Fig. 4B–D)."

eidos
"As shown above , LPS induced USP13 degradation in 24 h , but not in a shorter time exposure ( 4 and 8 h ) ."

reach
"Inhibition of proteasome, but not lysosome or immunoproteasome, attenuates LPS-induced USP13 degradation, suggesting USP13 degradation is mediated by the ubiquitin-proteasome system."

eidos
"LPS induces USP13 degradation by the ubiquitin-proteasome system in Kupffer cells ."

reach
"LPS reduces USP13 protein stability, not transcription, in kupffer cells."

reach
"Lipopolysaccharide reduces USP13 stability through c-Jun N-terminal kinase activation in Kupffer cells."
Lipopolysaccharide decreases the amount of USP13.
| 9
Lipopolysaccharide decreases the amount of USP13. 9 / 9
| 9

reach
"Furthermore, LPS reduced USP13 mRNA levels in Raw264.7 cells (L. Li et al., 2019)."

reach
"We found that LPS treatment of Raw264.7 cells diminished USP13 levels in both dose- and time-dependent manners (Fig. 1 H–K), consistent with our previous study."

reach
"We have shown that LPS treatment decreased USP13 levels in mouse macrophage-like RAW264.7 cells ; however, the molecular regulation of USP13 during the development of pulmonary fibrosis has not been investigated."

reach
"The western blotting further confirmed that LPS reduces USP13 protein levels without altering its transcription (Fig. 3B), suggesting that LPS may affect USP13 protein stability."

reach
"LPS treatment starting from 10 ng/ml decreased USP13 levels after 24 h (Fig. 2A–D)."

reach
"To further investigate molecular mechanisms of USP13 reduction in an inflammatory condition, we confirmed our published data and showed that intratracheal LPS challenge diminished USP13 protein levels in murine lungs (Fig. 1A and B)."

reach
"We have shown that LPS reduced USP13 mRNA expression in Raw264.7 cells."

reach
"We revealed that LPS reduces USP13 protein levels in both cell types, while USP13 mRNA is reduced in Raw264.7 cells, but not in Kupffer cells."

reach
"To investigate the molecular mechanisms by which LPS reduces USP13 levels in macrophages in the liver, we examined the effect of LPS on USP13 levels in Kupffer cells."
Lipopolysaccharide ubiquitinates USP13.
| 2
Lipopolysaccharide leads to the ubiquitination of USP13. 2 / 2
| 2

reach
"Furthermore, LPS increases USP13 polyubiquitination."

reach
"We found that short time exposure of LPS did not trigger USP13 degradation, suggesting that an immediate LPS signaling through TLR4 does not contribute to USP13 ubiquitination and degradation."
| 2

reach
"As shown in Fig. 4A, LPS increased USP13 lysine (K)48-linked polyubiquitination."

reach
"LPS-induced USP13 degradation was attenuated by actinomycin D (Fig. 6), suggesting a new protein induced by LPS regulates USP13 ubiquitination."
USP13 affects Cohesin
| 1 11 18
USP13 binds Cohesin.
| 1 9 17
| 1 9 17

sparser
"To test this, 293T cells were arrested at different points in the cell cycle and the interaction between cohesin and USP13 measured by performing USP13 Western blots on cohesin immunoprecipitates."

sparser
"We wondered whether ubiquitinated cohesin subunits were present primarily in the soluble fraction (where the USP13cohesin complex resides), in the chromatin fraction, or both."

sparser
"Mass spectrometry analysis revealed that USP13 could interact with Cohesin, and USP13 functioned as both ubiquitinase and deubiquitinase for Cohesin subunits."

sparser
"These data indicate that the interaction of USP13 with cohesin is cell cycle dependent—it is enhanced during DNA replication"

reach
"To test this, 293T cells were arrested at different points in the cell cycle and the interaction between cohesin and USP13 measured by performing USP13 Western blots on cohesin immunoprecipitates."

reach
"As shown in XREF_FIG D, the interaction between cohesin and USP13 was enhanced in cells arrested in the S phase when compared with asynchronous cycling cells, or cells arrested at other points in the cell cycle."

sparser
"USP13 interacts with soluble cohesin complexes preferentially during DNA replication."

reach
"At this point we had demonstrated that endogenous USP13 bound to endogenous cohesin in the nucleus of human cells."

sparser
"Endogenous USP13 was also robustly coimmunoprecipitated by SMC3, confirming the interaction between USP13 and cohesin in genetically unmodified cells."

reach
"Endogenous USP13 was also robustly coimmunoprecipitated by SMC3, confirming the interaction between USP13 and cohesin in genetically unmodified cells."
USP13 ubiquitinates Cohesin.
| 1 1
USP13 ubiquitinates Cohesin. 2 / 2
| 1 1

reach
"Finally, USP13 dependent ubiquitination of cohesin is required for the dissociation of cohesin from chromatin during mitosis."

sparser
"Finally, USP13-dependent ubiquitination of cohesin is required for the dissociation of cohesin from chromatin during mitosis."
USP13 deubiquitinates Cohesin.
| 1
USP13 leads to the deubiquitination of Cohesin on T293. 1 / 1
| 1

reach
"To investigate whether USP13 mediated the deubiquitination of cohesin subunits, we cotransfected 293T cells with expression vectors for His ubiquitin and Myc-USP13, purified ubiquitinated proteins by Ni-NTA affinity chromatography, and performed Western blot with antibodies to SMC3 and RAD21 (XREF_FIG A)."
PTEN affects USP13
8 | 9 11
PTEN binds USP13.
8 | 8 11
8 | 8 10

reach
"Considering that loss of PTEN contributed to fibroblasts phenotypic changes and USP13 interacted with PTEN and functioned as a deubiquitylase in epithelial cells, we subsequently sought to determine whether USP13 deficiency is responsible for the low PTEN expression observed in IPF fibroblasts."

sparser
"Moreover, purified GST-USP13, but not the GST-GFP control, was able to bind to FLAG-tagged PTEN under cell-free conditions ( xref ), which demonstrated a direct interaction between USP13 and PTEN."

sparser
"In human breast cancer, loss of USP13 is highly associated with loss of PTEN."

reach
"Interestingly, we found that USP13 interacted with PTEN in fibroblasts and regulated PTEN ubiquitination and degradation, supporting the idea that IPF is a cancer like disease."

reach
"We sought to determine whether USP13 directly interacts with PTEN and functions as a bona fide PTEN deubiquitinase."

reach
"Mechanistically, USP13 directly interacted with PTEN."

reach
"To further validate the interaction between USP13 and PTEN, an immunofluorescent confocal microscopic analysis was conducted."

sparser
"Additionally, USP13 interacted with the tumor suppressor phosphatase and tensin homolog (PTEN)."

reach
"Recently, a new study found that USP13 interacted with PTEN in fibroblasts and regulated PTEN ubiquitination and degradation."

sparser
"Co-immunoprecipitation assay was performed to assess the interaction between USP13 and PTEN protein."
USP13 binds OTUD3 and PTEN. 1 / 1
| 1

sparser
"USP7, a highly expressed DUB in prostate cancer and progressive multifocal leukoencephalopathy (PML), plays a direct role in PTEN deubiquitination and regulates its localization rather than protein stability. xref In addition, USP13 and OTUD3 can interact with PTEN and remove its polyubiquitin chain."
PTEN decreases the amount of USP13.
| 1
PTEN decreases the amount of USP13. 1 / 1
| 1

reach
"Expression of USP13 (but not the C345A mutant) in MDA-MB-231 cells, which led to upregulation of PTEN and downregulation of phospho-AKT and phospho-FOXO1/3 (XREF_FIG), significantly inhibited cell proliferation (XREF_FIG), colony formation on soft agar (XREF_FIG), lactate production (XREF_FIG), glucose uptake (XREF_FIG) and tumor growth (XREF_FIG), while knockdown of PTEN (XREF_SUPPLEMENTARY) rescued the proliferation of USP13 overexpressing MDA-MB-231 cells (XREF_FIG)."
USP13 affects UIMC1
1 | 27
USP13 deubiquitinates UIMC1.
1 | 18
USP13 deubiquitinates UIMC1. 10 / 19
1 | 18

reach
"Together with results showing USP13 is important for DDR and RAP80 localization at the sites of DNA damage, we hypothesized that RAP80 ubiquitination is inhibitory of its function, and deubiquitination of RAP80 by USP13 following DNA damage promotes RAP80 function in the DDR pathway."

reach
"For example, deubiquitination of RAP80 by USP13 facilitates the interaction between RAP80 and polyubiquitin chain and is important for RAP80-BRCA1 foci formation and DNA repair [117]."

reach
"USP13 deubiquitinates receptor associated protein 80 (RAP80) and promotes DNA damage response."

reach
"USP13, in turn, deubiquitinates RAP80 and promotes RAP80 recruitment and proper DDR."

reach
"Deubiquitination of RAP80 by USP13 is important for DDR."

reach
"These results suggest that deubiquitination of RAP80 by USP13 following DNA damage facilitates the binding between RAP80 and K63- linked polyubiquitin chain."

reach
"We demonstrated that polyubiquitination of RAP80 blocks its interaction with polyubiquitin chain and deubiquitination of RAP80 by USP13 facilitate the interaction between RAP80 and polyubiquitin chain."

reach
"In addition, USP13 specific inhibitor, Spautin-1, significantly enhanced RAP80 ubiquitination in control but not USP13 deficient cells (XREF_FIG)."

reach
"USP13 interacts with and deubiquitinates RAP80."

"USP13, in turn, deubiquitinates RAP80 and promotes RAP80 recruitment and proper DDR."
USP13 binds UIMC1.
| 6
| 6

reach
"USP13 interacts with and deubiquitinates RAP80."

reach
"Consistent with their reports, we also found that RAP80 binded with USP13 in EC cells treated with or without cisplatin."

reach
"Also, USP13 interacts to and deubiquitylates receptor-associated protein 80 (RAP80) following DNA damage to activate its ability, promoting DNA-damage responses ."

reach
"To evaluate the recruitment of USP13 and RAP80 at DSBs, we analysed the kinetics of GFP-USP13 and GFP-RAP80 accumulation on stripes after laser microirradiation."

reach
"Also, USP13 interacts with and deubiquitylates RAP80 to activate its function ."

reach
"To test our hypothesis, we first examined whether USP13 interacts with RAP80."
USP13 ubiquitinates UIMC1.
| 3
USP13 leads to the ubiquitination of UIMC1. 2 / 2
| 2

reach
"As shown in XREF_FIG, USP13 deficiency dramatically elevated the ubiquitination of RAP80 in vivo."

reach
"We found that mutating the 3 sites (K75, K90 and K112, 3KR) near or at the UIM domains, but not the other sites, abolished USP13 deficiency induced RAP80 ubiquitination (XREF_FIG and XREF_SUPPLEMENTARY)."
USP13 leads to the ubiquitination of ubiquitinated UIMC1. 1 / 1
| 1

reach
"Furthermore, reconstitution of USP13 WT but not the T196A fully reversed the increase in RAP80 ubiquitination induced by USP13 deficiency (XREF_SUPPLEMENTARY), suggesting USP13 phosphorylation by ATM is important for RAP80 deubiquitination."
USP13 affects AMFR
3 | 6 14
USP13 binds AMFR.
3 | 4 14
3 | 4 11

sparser
"Interestingly, USP13 associates with gp78 to attenuate polyubiquitination of ubiquitin-like protein 4A (UBL4A), a part of the Bag6 complex that helps chaperone retrotranslocated ERAD substrates to the proteasome, thereby protecting the Bag6 complex from proteasomal degradation and maintaining an adequate ERAD pathway, highlighting the importance of the associated DUB activity during ERAD [ xref ]."

sparser
"Several lines of evidence confirmed that gp78 could bind to USP13 directly and promote its interaction with p97."

sparser
"It appears that the second UBA domain is crucial for USP13 to bind gp78."

reach
"Several lines of evidence confirmed that gp78 could bind to USP13 directly and promote its interaction with p97."

No evidence text available

sparser
"Importantly, USP13 associates with AMFR to prevent polyubiquitylation of UBL4A, protecting the BAG6 complex from proteasomal degradation and maintaining proper ERAD pathway function [ xref ]."

sparser
"The requirement of gp78 interaction for ERAD inhibition by ectopic USP13 indicates that the interaction of USP13 with gp78 is functionally relevant to ERAD."

sparser
"Recently, cleaved Bag6 induced by caspase three activation was reported to interact with LC3-Ⅰ and pro-LC3 under ER stress conditions such as gp78 overexpression and USP13 downregulation, leading to autophagy inhibition and apoptosis activation ( xref )."

reach
"An endogenous interaction between USP13 and gp78 was also detected (XREF_FIG)."

sparser
"An endogenous interaction between USP13 and gp78 was also detected ( xref )."
USP13 binds BAG6 and AMFR. 2 / 2
| 2

sparser
"USP13 forms a multiprotein complex with gp78 and Bag6."

sparser
"Since Bag6 can interact with both gp78 and USP13, we asked whether these proteins could form a multiprotein complex."
USP13 binds VCP and AMFR. 1 / 1
| 1

sparser
"These results demonstrated a specific and direct interaction between USP13 and gp78 that facilitates USP13-p97 association."
USP13 inhibits AMFR.
| 1
USP13 inhibits AMFR. 1 / 4
| 1

reach
"Because the accumulation of Bag6 * upon USP13 depletion is significantly reduced in cells co-depleted of USP13 and gp78 (XREF_FIG), we propose that USP13 is required to antagonize a promiscuous activity of gp78 towards Ubl4A, which would otherwise impair the function of the Bag6 complex by altering its interaction pattern and/or increasing its cleavage by a cellular protease."
USP13 deubiquitinates AMFR.
| 1
USP13 leads to the deubiquitination of AMFR. 1 / 1
| 1

reach
"The proper function of BAG6 seems to require the activity of the deubiquitinating enzyme USP13, which antagonizes gp78 mediated ubiquitination to ensure ERAD efficiency."
Cohesin affects USP13
| 1 9 17
| 1 9 17

sparser
"To test this, 293T cells were arrested at different points in the cell cycle and the interaction between cohesin and USP13 measured by performing USP13 Western blots on cohesin immunoprecipitates."

sparser
"We wondered whether ubiquitinated cohesin subunits were present primarily in the soluble fraction (where the USP13cohesin complex resides), in the chromatin fraction, or both."

sparser
"Mass spectrometry analysis revealed that USP13 could interact with Cohesin, and USP13 functioned as both ubiquitinase and deubiquitinase for Cohesin subunits."

sparser
"These data indicate that the interaction of USP13 with cohesin is cell cycle dependent—it is enhanced during DNA replication"

reach
"To test this, 293T cells were arrested at different points in the cell cycle and the interaction between cohesin and USP13 measured by performing USP13 Western blots on cohesin immunoprecipitates."

reach
"As shown in XREF_FIG D, the interaction between cohesin and USP13 was enhanced in cells arrested in the S phase when compared with asynchronous cycling cells, or cells arrested at other points in the cell cycle."

sparser
"USP13 interacts with soluble cohesin complexes preferentially during DNA replication."

reach
"At this point we had demonstrated that endogenous USP13 bound to endogenous cohesin in the nucleus of human cells."

sparser
"Endogenous USP13 was also robustly coimmunoprecipitated by SMC3, confirming the interaction between USP13 and cohesin in genetically unmodified cells."

reach
"Endogenous USP13 was also robustly coimmunoprecipitated by SMC3, confirming the interaction between USP13 and cohesin in genetically unmodified cells."
BECN1 affects USP13
| 12 13
BECN1 binds USP13.
| 7 13
| 7 11

sparser
"Intriguingly, a previous study revealed that USP13 and Beclin1 could interact with each other."

sparser
"Furthermore, Beclin1 also interacts with USP13 and regulates the stability of USP13."

sparser
"Since ΔC-Beclin1 can bind to Vps34 ( xref ) but not USP13, this experiment suggests that the interaction of Beclin1 and USP13 is critically important for regulating the stability of Vps34 complexes in response to spautin-1 treatment."

reach
"Since the interaction of USP13 and Beclin1 is detectable by coimmunoprecipitation, we propose that Beclin1 may have close interaction with USP13."

sparser
"Since the interaction of USP13 and Beclin1 is detectable by coimmunoprecipitation, we propose that Beclin1 may have close interaction with USP13."

sparser
"We found that endogenous Beclin1 can interact with USP13 and the interaction was reduced in the presence of spautin-1 ( xref )."

sparser
"Because a direct interaction of USP13 with Beclin 1 has been detected, it is thought that USP13 counteracts the ubiquitination of Beclin 1 by yet unknown ligases [ xref ]."

sparser
"These data suggest that Beclin1 may closely interact with USP13, whereas its interaction with USP10 is indirect or transient in nature."

reach
"These data suggest that Beclin1 may closely interact with USP13, whereas its interaction with USP10 is indirect or transient in nature."

reach
"Since DeltaC-Beclin1 can bind to Vps34 but not USP13, this experiment suggests that the interaction of Beclin1 and USP13 is critically important for regulating the stability of Vps34 complexes in response to spautin-1 treatment."
| 2

sparser
"To determine the mechanism by which Vps34 complexes regulate the stability of USP13 and USP10, we examined the possibility that Beclin1 may interact with USP10 and USP13."

sparser
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."
BECN1 activates USP13.
| 3
BECN1 activates USP13. 3 / 3
| 3

reach
"The close functional interconnection between the central PI3K-III subunits and the DUBs is demonstrated by the interesting observation that knock-down of VPS34 and Beclin 1 causes instability of USP10 and USP13, which strongly indicates the existence of a regulatory feedback loop [XREF_BIBR]."

reach
"Taken together, these results suggest that Beclin1 is the primary target of USP10 and USP13."

reach
"Another interesting observation made in the Spautin-1 study is that knock-down of VPS34 and Beclin 1 causes instability of USP10 and USP13, which indicates the existence of a regulatory feedback loop [XREF_BIBR]."
BECN1 inhibits USP13.
| 1
BECN1 inhibits USP13. 1 / 1
| 1

reach
"It is interesting to note that the knock-down of VPS34 and BECLIN 1 also causes the degradation of USP10 and USP13, which indicates the existence of a regulatory feedback loop [XREF_BIBR]."
BECN1 increases the amount of USP13.
| 1
BECN1 increases the amount of USP13. 1 / 1
| 1

reach
"Unexpectedly, we found that the knockdown of Beclin1 or Vps34 could also reduce the endogenous levels of USP10 and USP13 (XREF_FIG)."

reach
"USP13 knockdown in BC cells promoted their proliferation, invasion and migration."

reach
"Treatment with afatinib alone diminished the number of proliferating cells to approximately 36%, while USP13 shRNA only slightly decreased cell proliferation."

reach
"By contrast, KD of USP13 only modestly reduced proliferation of cell lines with low USP13 expression (SKOV3 and IGROV1) (XREF_FIG; XREF_SUPPLEMENTARY)."

reach
"Next, we treated infected THP-1 cells with a cell-permeable potent autophagy inhibitor, spautin-1.58 Spautin-1 promotes the degradation of BECN1 by inhibiting 2 USPs (ubiquitin-specific peptidases), USP10 and USP13, which target BECN1.58 Spautin-1 treatment of infected cells significantly decreased E. chaffeensis proliferation (Fig. 3B)."

reach
"As a results, downregulation of USP13 dramatically inhibited A549 and H226 cell proliferation by AKT and MAPK signaling and suppressed tumor growth in nude mice."

reach
"Overexpression of miR-130b/301b or USP13 knockdown by shRNA delivery decreases USP13 expression, and further reduces PTEN protein expression and leads to enhancement of cell proliferation, invasion and migration."

reach
"Moreover, knockdown of USP13 promoted the proliferation of HCT116 colon cancer cells but not the isogenic PTEN-null HCT116 cells (XREF_FIG)."

reach
"Functional studies demonstrated that overexpression of USP13 suppressed OSCC cell proliferation, glucose uptake and lactate production in vitro and inhibited tumor growth in vivo."

reach
"Meanwhile, silencing of USP13 greatly enhanced proliferation, invasion and migration of the tested cells."

reach
"Depletion of USP13 inhibited cervical cancer cell proliferation."

reach
"Loss of USP13 in breast cancer cells promotes AKT phosphorylation, cell proliferation, anchorage independent growth, glycolysis and tumor growth through downregulation of PTEN."

reach
"Loss of USP13 in breast cancer cells promotes AKT phosphorylation, cell proliferation, anchorage independent growth, glycolysis and tumour growth through downregulation of PTEN."

reach
"Overexpression of miR-130b/301b or USP13 knockdown by shRNA delivery decreases USP13 expression, and further reduces PTEN protein expression and leads to enhancement of cell proliferation, invasion and migration."

reach
"In addition, USP13 disruption significantly reduced GSC proliferation (XREF_FIG) but showed little effects on NSTCs and NPCs (not depicted)."

reach
"In lung and ovarian cancers, where USP13 also regulates Mcl-1 stability, USP13 does not contribute to the cell proliferation of unstressed cells."

reach
"Further studies will be required to identify these targets and understand how they contribute to USP13 mediated cell proliferation."

reach
"XREF_FIG, USP13 deficiency significantly increased fibroblast proliferation."

reach
"Functionally, USP13 depletion led to decreased cell proliferation measured by two-dimensional (2D) colony formation and three-dimensional (3D) anchorage-independent growth."

reach
"Interestingly, despite the fact that the viral oncogenes do not regulate USP13 expression, depletion of USP13 in HPV- C33A cells did not cause a proliferation defect."

reach
"Furthermore, re-expression of cyclin D1 partially reversed the cell cycle arrest and cell proliferation inhibition induced by USP13 depletion in GC cells."

reach
"Two independent USP13 shRNAs (XREF_FIG) both markedly increased the proliferation (XREF_FIG) and anchorage independent growth (XREF_FIG) of SUM159 breast cancer cells, while restoration of PTEN (XREF_FIG) or expression of an RNAi resistant USP13 mutant (XREF_SUPPLEMENTARY) completely reversed the effect of USP13 shRNA (XREF_FIG)."

reach
"CK2-Mediated Phosphorylation Upregulates the Stability of USP13 and Promotes Ovarian Cancer Cell Proliferation."

reach
"Stable knockdown (KD) of USP13 by lentiviral short hairpin RNAs (shRNAs) markedly inhibited the proliferation of the USP13 amplified cell lines (CAOV3 and HeyA8) and the USP13 overexpressing cell line (OVCAR8)."
USP13 affects STING1
1 1 | 13 10
USP13 binds STING1.
1 | 7 10
1 | 7 9

sparser
"Here, we perform an unbiased screen by coimmunoprecipitation assays in cells cotransfected with FLAG-tagged DUBs and HA-STING, and find that USP13 interacts with STING."

reach
"Here, we perform an unbiased screen by coimmunoprecipitation assays in cells cotransfected with FLAG tagged DUBs and HA STING, and find that USP13 interacts with STING."

reach
"In contrast, USP5 which shares 80% sequence similarity with USP13, neither interacted with STING in an overexpression system nor inhibited cGAS and STING induced activation of IFN-beta promoter in reporter assays (XREF_FIG), indicating a specific interaction between USP13 and STING."

sparser
"USP13, a deubiquitinating enzyme, interacts with STING and catalyzed removal of K27 O- or K33 O-linked but not K27 R-linked polyubiquitin chains from STING."

sparser
"In contrast, USP5 which shares 80% sequence similarity with USP13, neither interacted with STING in an overexpression system nor inhibited cGAS and STING-induced activation of IFN-β promoter in reporter assays ( xref ), indicating a specific interaction between USP13 and STING."

sparser
"Since USP13 interacts with STING, an adaptor protein critically involved in innate antiviral responses, we investigated the role of USP13 in virus-triggered signalling in THP-1 cells by shRNA-mediated knockdown of USP13 ( xref )."

reach
"USP13 interacts with STING."

sparser
"These data together suggest that USP13 interacts with STING under physiological conditions."

reach
"Since USP13 interacts with STING, an adaptor protein critically involved in innate antiviral responses, we investigated the role of USP13 in virus triggered signalling in THP-1 cells by shRNA mediated knockdown of USP13 (XREF_FIG)."

reach
"Interestingly, SeV infection transiently disrupted USP13 STING association, whereas HSV-1 infection impaired but not totally abolished the interaction between USP13 and STING (XREF_FIG)."
| 1

sparser
"In support of this notion, we observed that (i) USP13 deficiency in mice or knockdown of USP13 in human cells potentiated HSV-1-triggered activation of IRF3 and NF-κB and subsequent induction of type I IFNs and proinflammatory cytokines, (ii) USP13 deficient cells restricted HSV-1 replication more profoundly than did the wild-type controls and USP13 deficient mice were more resistant to lethal HSV-1 infection compared to the wild-type littermates, (iii) USP13 but not USP13(AE) removed K27-linked ubiquitination of STING in cells or in vitro , and (iv) overexpression of USP13 specifically disrupted TBK1-STING association and knockdown or knockout of USP13 potentiated STING-TBK1 association after HSV-1 infection."
USP13 ubiquitinates STING1.
| 4
USP13 ubiquitinates STING1. 4 / 4
| 4

reach
"Nevertheless, USP13 deficiency enhances antiviral responses through deubiquitinating stimulator of interferon (STING) [56]."

reach
"Consistently, reconstitution of USP13 but not USP13 (AE) into Usp13 m/m MEFs inhibited HSV-1-induced ubiquitination of STING (XREF_FIG)."

reach
"These data together suggest that USP13 inhibits the recruitment of TBK1 to STING by deubiquitinating STING."

reach
"USP13 negatively regulates antiviral responses by deubiquitinating STING."
USP13 deubiquitinates STING1.
1 | 2
USP13 leads to the deubiquitination of STING1. 2 / 2
1 | 1

reach
"Consistently, reconstitution of USP13 but not USP13 (AE) into Usp13 m/m MEFs inhibited HSV-1-induced ubiquitination of STING (XREF_FIG)."

"Here, we show that ubiquitin-specific protease 13 (USP13) is a STING-interacting protein that catalyses deubiquitination of STING."
Mutated USP13 deubiquitinates STING1. 1 / 1
| 1

reach
"As expected, we found that USP13 but not the enzymatic inactive mutant USP13 (AE) catalysed deubiquitination of STING in cells or in vitro (XREF_FIG)."
AMFR affects USP13
3 | 5 14
AMFR binds USP13.
3 | 4 14
3 | 4 11

sparser
"Interestingly, USP13 associates with gp78 to attenuate polyubiquitination of ubiquitin-like protein 4A (UBL4A), a part of the Bag6 complex that helps chaperone retrotranslocated ERAD substrates to the proteasome, thereby protecting the Bag6 complex from proteasomal degradation and maintaining an adequate ERAD pathway, highlighting the importance of the associated DUB activity during ERAD [ xref ]."

sparser
"Several lines of evidence confirmed that gp78 could bind to USP13 directly and promote its interaction with p97."

sparser
"It appears that the second UBA domain is crucial for USP13 to bind gp78."

reach
"Several lines of evidence confirmed that gp78 could bind to USP13 directly and promote its interaction with p97."

No evidence text available

sparser
"Importantly, USP13 associates with AMFR to prevent polyubiquitylation of UBL4A, protecting the BAG6 complex from proteasomal degradation and maintaining proper ERAD pathway function [ xref ]."

sparser
"The requirement of gp78 interaction for ERAD inhibition by ectopic USP13 indicates that the interaction of USP13 with gp78 is functionally relevant to ERAD."

sparser
"Recently, cleaved Bag6 induced by caspase three activation was reported to interact with LC3-Ⅰ and pro-LC3 under ER stress conditions such as gp78 overexpression and USP13 downregulation, leading to autophagy inhibition and apoptosis activation ( xref )."

reach
"An endogenous interaction between USP13 and gp78 was also detected (XREF_FIG)."

sparser
"An endogenous interaction between USP13 and gp78 was also detected ( xref )."
USP13 binds BAG6 and AMFR. 2 / 2
| 2

sparser
"USP13 forms a multiprotein complex with gp78 and Bag6."

sparser
"Since Bag6 can interact with both gp78 and USP13, we asked whether these proteins could form a multiprotein complex."
USP13 binds VCP and AMFR. 1 / 1
| 1

sparser
"These results demonstrated a specific and direct interaction between USP13 and gp78 that facilitates USP13-p97 association."
AMFR activates USP13.
| 1
AMFR activates USP13. 1 / 2
| 1

reach
"Since purified gp78 is insufficient to activate USP13 (Liu, Y unpublished results), further experiments using gp78 containing retrotranslocation complexes reconstituted in proteoliposome are required to elucidate how USP13 is regulated in the context of retrotranslocation."
Spautin-1 affects USP13
1 | 2 19
Spautin-1 inhibits USP13.
1 | 2 12
1 | 2 12

No evidence text available

eidos
"Spautin-1 also reduced USP13 expression , as has been previously observed in other cell lines ( Fig. 8A ) ."

reach
"Indeed, a recent study showed that a small molecule, Spautin-1, promoted the degradation of Vps34 by inhibiting two ubiquitin specific proteases USP10 and USP13 that regulate the stability of the Vps34 complex [XREF_BIBR]."

reach
"Spautin-1 inhibits ubiquitin specific peptidases, USP10 and USP13, and promotes the degradation of Vps34-PI3 kinase complexes, key regulators of autophagy, leading to inhibition of autophagy."

reach
"A study by Liu et al. (Liu et al., 2011) has demonstrated that Spautin-1 is a potent small-molecule inhibitor of USP10 and USP13, and treatment with Spautin-1 inhibits the deubiquitinase activity of USP10 and USP13, resulting in an increasing ubiquitination and accelerating degradation of Beclin1 in Vps34 complexes, ultimately inhibiting autophagy."

reach
"58 Spautin-1 promotes the degradation of BECN1 by inhibiting 2 USPs (ubiquitin-specific peptidases), USP10 and USP13, which target BECN1."

reach
"Spautin-1 inhibits autophagy by reducing the activity of USP10 and USP13, which promotes a major aspect of autophagy: degradation by Vps34 complexes."

reach
"Molecular analysis revealed that Spautin-1 directly inhibits the activity of the deubiquitinating enzymes (DUBs) ubiquitin specific protease 10 (USP10) and USP13."

reach
"Spautin-1 inhibits the activity of the deubiquitinating enzymes USP13 and USP10."

reach
"Spautin-1 was shown to inhibit USP-13, a de-ubiquitinase of the host cell hijacked by the SARS-CoV-2 which led to successful ubiquitination of nsp-13 [109]."
Spautin-1 activates USP13.
| 6
Spautin-1 activates USP13. 6 / 6
| 6

reach
"Spautin-1 reportedly suppresses autophagy by inhibiting the USP10 and USP13 deubiquitinases."

reach
"Spautin-1 targets the activity of two ubiquitin specific peptidases, USP10 and USP13 and causes PI3K complex degradation."

reach
"USP13 inhibitor, Spautin-1, treatment also impaired HR in control but not USP13 deficient cells (XREF_SUPPLEMENTARY)."

reach
"Spautin-1 reportedly inhibits autophagosomal initiation and maturation by inhibiting deubiquitylases USP10 and USP13, which stabilize the Beclin-1 and VPS34 complex (26)."

reach
"The Autophagy Inhibitor Spautin-1 Antagonizes Rescue of Mutant CFTR Through an Autophagy-Independent and USP13-Mediated Mechanism."

reach
"Spautin-1 has been recently reported to prevent autophagy by inhibiting the deubiquitinases USP10 and USP13, which leads to beclin 1 and Vps34 (or PI3KIII) degradation [XREF_BIBR]."
Spautin-1 decreases the amount of USP13.
| 1
Spautin-1 decreases the amount of USP13. 1 / 1
| 1

reach
"Spautin-1 also reduced USP13 expression, as has been previously observed in other cell lines."
USP13 affects USP10
1 | 10 7
USP13 binds USP10.
| 4 7
| 4 3

sparser
"USP13 also interacts with USP10, and the interaction increases USP13 deubiquitinating activity ( xref )."

reach
"Although no structural information for USP13 and USP10 is currently available, the enhanced DUB activity when USP13 interacts with USP10 or when they interact with Beclin1 suggest that the interaction of USP13 and USP10 with each other or with their substrates can lead to changes in their conformation which may be critical for the catalytic activities."

reach
"Using spautin-1 as a tool, we explored the interaction of USP10 and USP13 with Vps34 complexes."

reach
"USP13 also interacts with USP10, and the interaction increases USP13 deubiquitinating activity."

sparser
"Although no structural information for USP13 and USP10 is currently available, the enhanced DUB activity when USP13 interacts with USP10 or when they interact with Beclin1 suggest that the interaction of USP13 and USP10 with each other or with their substrates can lead to changes in their conformation which may be critical for the catalytic activities."

reach
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."

sparser
"No orthogonal biophysical methods, such as the surface plasmon response (SPR) or isothermal titration calorimetry (ITC), were used to validate the binding of spautin-1 with USP10 or USP13."
| 2

sparser
"To determine the mechanism by which Vps34 complexes regulate the stability of USP13 and USP10, we examined the possibility that Beclin1 may interact with USP10 and USP13."

sparser
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."
SKP2 binds USP10 and USP13. 1 / 1
| 1

sparser
"The present study uncovers that USP10 is another critical DUB that deubiquitinates SKP2 and suppresses the ubiquitin-dependent proteasomal degradation of SKP2, which is compellingly supported by multiple lines of evidence: (1) our Co-IP experiments revealed compelling interaction of endogenous SKP2 with not only USP13 but also USP10 in KBM5-T315I and KBM5 cells (Fig.  xref ); (2) the interaction of overexpressed SKP2 with overexpressed USP13 or USP10 was also confirmed in HEK293T cells but the USP10 missing the N-terminal 205 amino acid residues failed to bind to SKP2 (Fig.  xref ); (3) overexpression of either USP13 or USP10 led to a higher SKP2 protein level and decreased p27 but overexpression of a DUB activity-disabled USP10 failed to do so (Fig.  xref ); (4) shRNA-mediated knockdown or pharmacological inhibition of USP10 with Spautin1 remarkable decreased SKP2 and phosphorylated Bcr-Abl (but not total Bcr-Abl) but increased p27 in multiple CML cell lines (Fig.  xref , Fig.  xref ); (5) CHX chase assays showed that shortened the halflife of SKP2 protein (Fig.  xref ); and (6) both pharmacological and genetic inhibition of USP10 could markedly increase K48-linked ubiquitinated SKP2 (Fig.  xref )."
| 1

sparser
"We also used an in vitro assay to test the effect of treating HA-Ub-conjugated FLAG-LC3B with recombinant His6-tagged USP10 (His6-USP10) or His6-tagged USP13 (His6-USP13) (specificity control)."
USP13 deubiquitinates USP10.
1 | 5
USP13 deubiquitinates USP10. 6 / 6
1 | 5

reach
"Moreover, beclin-1 and USP10 are involved in a potential feedforward mechanism in which beclin-1 stabilizes USP13, which in turn deubiquitinates and stabilizes USP10, leading to increased beclin-1 levels and activity."

No evidence text available

reach
"Since USP13 can also deubiquitinate USP10, regulating the stability of USP13 by Beclin1 provides a mechanism for Beclin1 to control the stability of USP10."

reach
"These results suggest that USP13 may directly regulate the deubiquitination of USP10; however, USP10 may regulate USP13 indirectly perhaps by affecting the levels of Vps34 complexes."

reach
"Very interestingly, they also pointed out that beclin-1 controlled the stability of USP10 by regulating the stability of USP13, which can deubiquitinate USP10 (also see Figure 4) [53]."

reach
"Consistent with this possibility, the ubiquitination levels of USP10 were reduced when cells were cotransfected with an expression vector of USP13 and the addition of spautin-1 inhibited the deubiquitination of USP10 by USP13 (XREF_FIG)."
USP13 inhibits USP10.
| 1
USP13 inhibits USP10. 1 / 1
| 1

reach
"It will be interesting to elucidate if the stability of the other PI3K and III complex members is also affected, as in the case of Spautin-1 -- mediated inhibition of USP10 and USP13 [XREF_BIBR] and if the control of Beclin 1 stability is mediated by HSP90 in general or whether this represents a regulatory event specifically developed in phagocytic cells."
MCL1 affects USP13
| 13 8
MCL1 binds USP13.
| 11 8
| 11 7

reach
"Therefore, we propose that USP13 is a bona fide deubiquitinase for MCL1, a notion that is further supported by the endogenous interaction and correlative expression of USP13 and MCL1 only at protein levels in clinical tumor samples."

reach
"Moreover, the interaction of USP13 and MCL1 occurred between endogenous proteins in A549, HEY and SW-1573 tumor cells."

reach
"We further conducted the in vitro immunoprecipitation experiment, the result of which suggested that the interaction between USP13 and MCL1 was direct and independent on MCL1 ubiquitination status."

reach
"USP13 interacts with and deubiquitinates MCL1."

sparser
"In vitro assays revealed the interaction of USP13 and MCL-1 and USP13 could deubiquitinate and stabilize MCL-1 ( xref ; xref )."

sparser
"We further conducted the in vitro immunoprecipitation experiment, the result of which suggested that the interaction between USP13 and MCL1 was direct and independent on MCL1 ubiquitination status (Fig.  xref )."

reach
"USP13 interacts with and deubiquitinates Mcl-1 in cervical cancer cells."

sparser
"Second, USP13 interacted with MCL1 and decreased MCL1 polyubiquitination in a DUB activity-dependent manner."

sparser
"However, correlation analysis of RNA sequencing data from TCGA failed to provide evidence that USP13 and MCL1 were associated with each other at the messenger RNA (mRNA) level (Supplementary Fig.  xref ), implying that USP13-mediated regulation of MCL1, if any, might not occur through a transcriptional mechanism."

reach
"To gain insights into USP13 mediated regulation of MCL1, we tested whether USP13 interacted with MCL1 and functioned as a deubiquitinase."
USP13 binds MCL1 and FlaG. 1 / 1
| 1

sparser
"Immunoprecipitation with anti-FLAG antibody demonstrated that FLAG-USP13 interacted with over-expressed Mcl-1 (Fig. xref )."
MCL1 activates USP13.
| 2
MCL1 activates USP13. 2 / 2
| 2

reach
"Here, we provided several lines of evidence to support that MCL1 was a deubiquitination target of USP13 in lung and ovarian cancer."

reach
"Interestingly, restoration of Mcl-1 expression only partially restored cell proliferation in USP13 depleted cervical cancer cells."
USP13 affects Ubiquitin
| 4 7
USP13 binds Ubiquitin.
| 7

sparser
"We firstly examined whether the ZnF domain of USP13 (USP13-ZnF) interacts with Ub by isothermal titration calorimetry (ITC) experiment ( xref )."

sparser
"The GST pull-down experiment indicates that full-length USP13 can bind with Ub, but not with ISG15, SUMO1 and SUMO2 ( xref )."

sparser
"The ZnF domain of USP13 cannot bind with ubiquitin, so that USP13 loses its ability to be activated by free ubiquitin ( xref )."

sparser
"The ZnF domain of USP13 cannot bind with Ub."
| 3

sparser
"The tandem UBA domains of USP13 interact with Ub."

sparser
"Moreover, the individual UBA domains (UBA1 and UBA2) of USP13 can also bind with Ub ( xref )."

sparser
"Notably, the tandem UBA domains of USP13 could bind to Ub and catalyze the hydrolysis of the polyUb chains (Lys-63/48/27) ( xref ; xref ; xref )."
USP13 inhibits Ubiquitin.
| 4
| 3

reach
"USP13 interacted with TLR4 and inhibited the ubiquitin mediated degradation of TLR4."

reach
"In a cell study, USP5 and USP13 were found to degrade ubiquitin chains inside stress granules, defined as clumps of protein or RNA created when cells are stressed [XREF_BIBR]."

reach
"3.5 USP13 inhibits the ubiquitin mediated degradation of mutant EGFR."
USP13 bound to TLR4 inhibits Ubiquitin. 1 / 1
| 1

reach
"USP13 interacted with TLR4 and inhibited the ubiquitin mediated degradation of TLR4."
USP13 affects EGFR
| 13 6
USP13 binds EGFR.
| 2 6
| 2 6

sparser
"The interaction of wild‐type EGFR with USP13 was hardly detectable, whereas USP13 coimmunoprecipitated with the EGFR ΔE746‐A750 and L858R mutants (Figure xref ), indicating that USP13 associates much more tightly with mutant than wild‐type EGFR."

sparser
"We then recapitulated the interaction between exogenous USP13 and EGFR in HEK293T cells."

sparser
"The fraction of mutant EGFR bound to USP13 was also enriched in those two types of ubiquitin chains (Figure xref )."

reach
"We found that USP13 inhibits mutant EGFR degradation by a process that leads to the accumulation of ubiquitinated (K48 and K63) EGFR, which is bound to c-Cbl and USP13."

sparser
"The association is dependent on the presence of c‐Cbl, as we observed that increased expression of c‐Cbl enhanced USP13‐mutant EGFR interaction."

sparser
"The isopeptidase‐independent mechanism of action proposed so far for USP13 relies on its UBA domains, which bind to ubiquitinated substrates, possibly inducing structural changes that stabilize them. xref Coincidentally, K63‐linked ubiquitin chains are required for EGFR sorting and degradation. xref This type of chains has also been reported as the preferred substrates for the USP13 UBA domains. xref We found that the UBA domains of USP13 were required for the binding to and stabilizing EGFR, as mutated UBA domains in USP13 resulted in a loss of USP13EGFR interaction and of EGFR stabilization."

reach
"We then recapitulated the interaction between exogenous USP13 and EGFR in HEK293T cells."

sparser
"By employing the USP13‐C345A mutant or versions mutated in UBA1, UBA2 or both, we established that the UBA domains' integrity is necessary for USP13 to bind to mutant EGFR (Figure xref )."
USP13 inhibits EGFR.
| 5
USP13 inhibits mutated EGFR. 3 / 3
| 3

reach
"23 In HEK293T cells expressing EGFR-DeltaE740-A746, USP13 antagonized the destabilization of mutant EGFR mediated by c-Cbl or Cbl-b."

reach
"We demonstrate that USP13 targeting leads to an increased sensitivity toward EGFR inhibition by both osimertinib and afatinib in NSCLC harboring the most common EGFR mutations (DeltaE746-A750 and L858R and T790M) while sparing EGFR wild-type cells and tumors."

reach
"Overall, we showed that USP13 abrogates sorting and degradation of ubiquitinated mutant EGFR, independent of its catalytic activity but requiring its UBA domains that are known to bind to K63-type ubiquitin chains preferentially."
USP13 inhibits EGFR. 2 / 2
| 2

reach
"38, 39 Given that USP13 targeting caused EGFR destabilization, some of the effects may be independent of the EGFR kinase activity as EGFR can suppress cell death in manners that are independent of its kinase activity."

reach
"Moreover, USP13 inhibition and silencing lead to a decreased half-life of the mutant EGFR protein in NSCLC cells, indicating that USP13 likely stabilizes EGFR."
USP13 activates EGFR.
| 4
USP13 activates mutated EGFR. 4 / 4
| 4

reach
"Targeting USP13 mediated drug tolerance increases the efficacy of EGFR inhibition of mutant EGFR in non small cell lung cancer."

reach
"37 USP13 is likely to stabilize mutant EGFR in a ubiquitinated state, and inhibition of USP13 destabilizes mutant EGFR and abrogates signaling of mutant EGFR in lung cancer cells."

reach
"We found that USP13 inhibits mutant EGFR degradation by a process that leads to the accumulation of ubiquitinated (K48 and K63) EGFR, which is bound to c-Cbl and USP13."

reach
"Combined targeting of USP13 and EGFR strongly reduces the viability of EGFR mutant lung cancer cells in vitro and in vivo while sparing non malignant (EGFR wild-type) lung epithelial cells."
USP13 dephosphorylates EGFR.
| 1
USP13 leads to the dephosphorylation of EGFR. 1 / 1
| 1

reach
"The current study reveals that Spautin-1, a known inhibitor of ubiquitin specific peptidase 10 (USP10) and USP13, inhibits EGFR phosphorylation and the activation of its downstream signaling."
USP13 decreases the amount of EGFR.
| 1
USP13 decreases the amount of EGFR. 1 / 1
| 1

reach
"USP13 downregulation with siRNA decreased the total protein levels of EGFR, indicating a clear connection between USP13 expression and EGFR protein levels."
USP13 affects BAG6
3 | 5 11
3 | 4 5

No evidence text available

No evidence text available

No evidence text available

sparser
"By contrast, the cytosolic chaperone Hsp90 was not co-precipitated ( xref ), demonstrating a specific interaction between USP13 and the Bag6 complex."

sparser
"Because depletion of gp78 did not affect the interaction of USP13 with Bag6 ( xref , lane 7 vs 5), USP13 might bind Bag6 directly."

reach
"Because depletion of gp78 did not affect the interaction of USP13 with Bag6 (XREF_FIG, lane 7 vs 5), USP13 might bind Bag6 directly."

reach
"We next tested whether USP13 could physically interact with Bag6 and/or its cofactors."

reach
"Taken together, these results suggest a direct interaction between USP13 and Bag6 that is mediated by the Bag6 UBL domain."

reach
"Since Bag6 can interact with both gp78 and USP13, we asked whether these proteins could form a multiprotein complex."

sparser
"However, given that USP13 can also interact physically with Bag6, it is also possible that USP13 may serve an adaptor function to promote Bag6–SGTA interaction."
USP13 binds BAG6 and AMFR. 2 / 2
| 2

sparser
"USP13 forms a multiprotein complex with gp78 and Bag6."

sparser
"Since Bag6 can interact with both gp78 and USP13, we asked whether these proteins could form a multiprotein complex."
USP13 binds BAG6 and UBL. 2 / 2
| 2

sparser
"On the other hand, the binding of USP13 with the UBL domain of Bag6 can be demonstrated using pulldown experiments ( xref )."

sparser
"Taken together, these results suggest a direct interaction between USP13 and Bag6 that is mediated by the Bag6 UBL domain."
USP13 binds BAG6 and ubiquitin-like. 1 / 1
| 1

reach
"Indeed, GST pull-down experiments using purified proteins showed that GST-USP13 bound Bag6, and the N-terminal ubiquitin like (UBL) domain in Bag6 was both necessary and sufficient for interaction with USP13 (XREF_FIG)."
GST binds USP13 and BAG6. 1 / 1
| 1

sparser
"Indeed, GST pull-down experiments using purified proteins showed that GST-USP13 bound Bag6, and the N-terminal ubiquitin-like (UBL) domain in Bag6 was both necessary and sufficient for interaction with USP13 ( xref )."
USP13 binds BAG6 and TBCB. 1 / 1
| 1

sparser
"We next tested whether USP13 could physically interact with Bag6 and/or its cofactors."
Ubiquitin affects USP13
| 2 8
Ubiquitin binds USP13.
| 7

sparser
"We firstly examined whether the ZnF domain of USP13 (USP13-ZnF) interacts with Ub by isothermal titration calorimetry (ITC) experiment ( xref )."

sparser
"The GST pull-down experiment indicates that full-length USP13 can bind with Ub, but not with ISG15, SUMO1 and SUMO2 ( xref )."

sparser
"The ZnF domain of USP13 cannot bind with ubiquitin, so that USP13 loses its ability to be activated by free ubiquitin ( xref )."

sparser
"The ZnF domain of USP13 cannot bind with Ub."
| 3

sparser
"The tandem UBA domains of USP13 interact with Ub."

sparser
"Moreover, the individual UBA domains (UBA1 and UBA2) of USP13 can also bind with Ub ( xref )."

sparser
"Notably, the tandem UBA domains of USP13 could bind to Ub and catalyze the hydrolysis of the polyUb chains (Lys-63/48/27) ( xref ; xref ; xref )."
Ubiquitin activates USP13.
| 2 1
| 2 1

reach
"Domain swapping confers USP13 the ability to be activated by Ub."

reach
"Since the ZnF domain of USP13 is not the Ub receptor, it is crucial to examine whether USP13 could be catalytically activated by Ub."

sparser
"Since the ZnF domain of USP13 is not the Ub receptor, it is crucial to examine whether USP13 could be catalytically activated by Ub."
USP13 affects VCP
2 | 7 9
USP13 binds VCP.
2 | 5 9
2 | 5 5

sparser
"By contrast, several other factors enhanced the p97-USP13 interaction."

No evidence text available

sparser
"The data also suggest that the interaction of USP13 with p97 is mediated by several cellular factors with gp78 being a major one."

sparser
"Secondly, depletion of gp78 by shRNA-mediated gene knockdown or gene deletion reduced the USP13-p97 interaction by threefold to fourfold ( xref , xref )."

sparser
"We used the GST pull-down assay to screen a collection of known p97 interactors in order to identify factor(s) that promotes the USP13-p97 interaction."

reach
"USP13 interacts with p97 and VCP, Ufd1, Npl4, and UBXD8 [XREF_BIBR]."

reach
"By contrast, neither GST-USP13 nor GST-USP5 bound p97 (XREF_FIG)."

No evidence text available

reach
"In addition, USP13 interacts with the P97 and valosin containing protein complex, a key chaperone in ER associated degradation, which increases the protein level of CD3delta, an ER associated degradation substrate."

reach
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
GST binds USP13 and VCP. 1 / 1
| 1

sparser
"We expressed these proteins in cells individually and examined the binding of endogenous p97 to GST-USP13."
GST binds USP13, USP5, and VCP. 1 / 1
| 1

sparser
"By contrast, neither GST-USP13 nor GST-USP5 bound p97 ( xref )."
USP13 binds VCP and AMFR. 1 / 1
| 1

sparser
"These results demonstrated a specific and direct interaction between USP13 and gp78 that facilitates USP13-p97 association."
| 1

sparser
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
USP13 inhibits VCP.
| 1
USP13 inhibits VCP. 1 / 1
| 1

reach
"Because loss of USP13 phenocopies loss of VCP we hypothesize that USP13 functions in regulating the activity of the VCP complex itself and/or other ubiquitin ligases that promote TCRalphaGFP turnover."
USP13 activates VCP.
| 1
USP13 activates VCP. 1 / 1
| 1

reach
"By contrast, USP13 depletion did not affect deglycosylation of TCRalpha (lane 2 vs 1), suggesting that USP13 promotes ERAD downstream of p97."
USP13 affects OGDH
2 1 | 8 3
USP13 binds OGDH.
2 | 3
2 | 1

No evidence text available

No evidence text available

sparser
"The N terminus including a ubiquitin-specific protease-type zinc-finger domain (residues 1–336) was essential for USP13 to interact with OGDH, whereas the ubiquitin carboxyl-terminal hydrolase domain (residues 336–863) of USP13 is required for its binding with ACLY ( xref )."
ACLY binds USP13 and OGDH. 2 / 2
| 2

sparser
"Reciprocal analyses in which immunoprecipitated ACLY or OGDH were probed for USP13 confirmed the USP13ACLY and USP13OGDH interactions ( xref )."

sparser
"Western blot analyses of HEK293T cell lysates immunoprecipitated with USP13 antibody showed the interaction of USP13 with ACLY and OGDH ( xref )."
USP13 deubiquitinates OGDH.
1 | 3
USP13 deubiquitinates OGDH. 4 / 4
1 | 3

"USP13, the main regulator of ovarian cancer energy metabolism, specifically deubiquitinates and stabilizes oxoglutarate dehydrogenase and ATP citrate lyase, which can catalyze fatty acid synthesis, glutaminolysis and mitochondrial respiration."

reach
"USP13 directly deubiquitinates ACLY and OGDH."

reach
"Furthermore, USP13 can deubiquitinate and stabilise ACLY and OGDH and c-Myc in ovarian cancer and glioblastoma, respectively, thereby functioning as an oncogene [XREF_BIBR, XREF_BIBR]."

reach
"Taken together, these studies suggest that USP14 is involved in the occurrence and progression of multiple malignant tumors.Other USP family members: USP13, the main regulator of ovarian cancer energy metabolism, specifically deubiquitinates and stabilizes oxoglutarate dehydrogenase and ATP citrate lyase, which can catalyze fatty acid synthesis, glutaminolysis and mitochondrial respiration."
USP13 activates OGDH.
| 2
USP13 activates OGDH. 2 / 4
| 2

reach
"Next, we examined whether USP13 augments the stability of ACLY and OGDH."

reach
"USP13 upregulates ACLY and OGDH, two key regulators that drive glutaminolysis, ATP generation and lipid synthesis in cancer metabolism."
USP13 inhibits OGDH.
| 2
USP13 inhibits OGDH. 2 / 3
| 2

reach
"These data suggest that USP13-KD induces mitochondrial dysfunction and lipogenic dysfunction by reducing OGDH and ACLY activity, thereby reducing glutamine 's reductive carboxylation and glucose 's oxidation for lipid synthesis (XREF_FIG)."

reach
"Depletion of USP13 markedly decreased protein stability of ACLY and OGDH (XREF_FIG; XREF_SUPPLEMENTARY), but had no effect on the transcription of ACLY and OGDH (XREF_FIG)."
USP13 decreases the amount of OGDH.
| 1
USP13 decreases the amount of OGDH. 1 / 2
| 1

reach
"We proposed that KD of USP13 reduces OGDH expression thereby limiting the synthesis of TCA cycle intermediates from oxidative pathways (XREF_FIG)."
STING1 affects USP13
1 | 7 10
1 | 7 9

sparser
"Here, we perform an unbiased screen by coimmunoprecipitation assays in cells cotransfected with FLAG-tagged DUBs and HA-STING, and find that USP13 interacts with STING."

reach
"Here, we perform an unbiased screen by coimmunoprecipitation assays in cells cotransfected with FLAG tagged DUBs and HA STING, and find that USP13 interacts with STING."

reach
"In contrast, USP5 which shares 80% sequence similarity with USP13, neither interacted with STING in an overexpression system nor inhibited cGAS and STING induced activation of IFN-beta promoter in reporter assays (XREF_FIG), indicating a specific interaction between USP13 and STING."

sparser
"USP13, a deubiquitinating enzyme, interacts with STING and catalyzed removal of K27 O- or K33 O-linked but not K27 R-linked polyubiquitin chains from STING."

sparser
"In contrast, USP5 which shares 80% sequence similarity with USP13, neither interacted with STING in an overexpression system nor inhibited cGAS and STING-induced activation of IFN-β promoter in reporter assays ( xref ), indicating a specific interaction between USP13 and STING."

sparser
"Since USP13 interacts with STING, an adaptor protein critically involved in innate antiviral responses, we investigated the role of USP13 in virus-triggered signalling in THP-1 cells by shRNA-mediated knockdown of USP13 ( xref )."

reach
"USP13 interacts with STING."

sparser
"These data together suggest that USP13 interacts with STING under physiological conditions."

reach
"Since USP13 interacts with STING, an adaptor protein critically involved in innate antiviral responses, we investigated the role of USP13 in virus triggered signalling in THP-1 cells by shRNA mediated knockdown of USP13 (XREF_FIG)."

reach
"Interestingly, SeV infection transiently disrupted USP13 STING association, whereas HSV-1 infection impaired but not totally abolished the interaction between USP13 and STING (XREF_FIG)."
| 1

sparser
"In support of this notion, we observed that (i) USP13 deficiency in mice or knockdown of USP13 in human cells potentiated HSV-1-triggered activation of IRF3 and NF-κB and subsequent induction of type I IFNs and proinflammatory cytokines, (ii) USP13 deficient cells restricted HSV-1 replication more profoundly than did the wild-type controls and USP13 deficient mice were more resistant to lethal HSV-1 infection compared to the wild-type littermates, (iii) USP13 but not USP13(AE) removed K27-linked ubiquitination of STING in cells or in vitro , and (iv) overexpression of USP13 specifically disrupted TBK1-STING association and knockdown or knockout of USP13 potentiated STING-TBK1 association after HSV-1 infection."
MYC affects USP13
| 7 8
MYC binds USP13.
| 6 8
| 6 4

sparser
"To investigate whether USP13 mediated the deubiquitination of cohesin subunits, we cotransfected 293T cells with expression vectors for His-ubiquitin and Myc-USP13, purified ubiquitinated proteins by Ni-NTA affinity chromatography, and performed Western blot with antibodies to SMC3 and RAD21 ( xref A )."

sparser
"Computational modeling of structures predicted in ZDOCK and Pymol software showed that Y708 phosphorylation was necessary for the direct binding of USP13 to c-Myc ( xref )."

sparser
"We then examined the physiological significance of USP13 phosphorylation by CLK3 at Y708. xref indicates that CLK3 knockdown in HuCCT1 cells strongly impaired the interaction of USP13 with c-Myc."

sparser
"As USP13 is a deubiquitinase, the interaction between USP13 and c-Myc led us to hypothesize that USP13 may stabilize c-Myc protein in GSCs through deubiquitination."

reach
"These results demonstrate that c-Myc interacts with USP13 and FBXL14 in glioma cells, indicating that deubiquitinase USP13 and the ubiquitin E3 ligase FBXL14 are potential regulators of c-Myc protein stability in GBM."

reach
"However, overexpression of CLK3 in HCCC9810 cells promoted the binding of c-Myc to WT-USP13, not USP13 Y708F mutant (XREF_FIG)."

reach
"USP13 and FBXL14 interact with c-Myc in glioma cells."

reach
"The interaction between c-Myc and USP13 or FBXL14 was validated in HEK293 cells coexpressing c-Myc and Flag tagged USP13 or FBXL14."

reach
"Computational modeling of structures predicted in ZDOCK and Pymol software showed that Y708 phosphorylation was necessary for the direct binding of USP13 to c-Myc (XREF_FIG)."

reach
"As USP13 is a deubiquitinase, the interaction between USP13 and c-Myc led us to hypothesize that USP13 may stabilize c-Myc protein in GSCs through deubiquitination."
USP13 binds FBXL14 and MYC. 4 / 4
| 4

sparser
"These results demonstrate that c-Myc interacts with USP13 and FBXL14 in glioma cells, indicating that deubiquitinase USP13 and the ubiquitin E3 ligase FBXL14 are potential regulators of c-Myc protein stability in GBM."

sparser
"The interaction between c-Myc and USP13 or FBXL14 was validated in HEK293 cells coexpressing c-Myc and Flag-tagged USP13 or FBXL14."

sparser
"To further confirm the interaction between endogenous c-Myc and USP13 or FBXL14 in GSCs, c-Myc was immunoprecipitated from cell lysate of partially differentiated GSCs that were induced by serum acutely (2 d)."

sparser
"USP13 and FBXL14 interact with c-Myc in glioma cells."
MYC activates USP13.
| 1
MYC activates USP13. 1 / 3
| 1

reach
"We found a positive correlation between TRF1 levels and all the stem cell markers with the exception of MYC, although TRF1 was positively correlated with the MYC modulator USP13 (Fang et al., 2017)."
ATM affects USP13
| 9 8
ATM phosphorylates USP13. 10 / 13
| 6 7

reach
"Mechanistically, USP13 is phosphorylated by ATM following DNA damage which, in turn, facilitates its DSB localization."

reach
"Together, these results suggest that USP13 phosphorylation by ATM is important for its recruitment to DSBs, DDR and chemo sensitivity."

sparser
"A recent study suggested that DNA damage could induce the phosphorylation of USP13 by the ATM and then activated USP13 deubiquitinated RAP80 (K75, K90, and K112)."

reach
"Taken together, these results demonstrate that USP13 phosphorylation by ATM is important for USP13 recruitment to DSB sites following DNA damage and proper DDR."

sparser
"Taken together, these results demonstrate that USP13 phosphorylation by ATM is important for USP13 recruitment to DSB sites following DNA damage and proper DDR."

sparser
"In response to DNA damage, USP13 is phosphorylated by ATM, which in turn facilitates USP13 recruitment to DSBs, RAP80 deubiquitination, and triggers DDR signalling."

reach
"USP13 is phosphorylated by ATM following DNA damage."

sparser
"Mechanistically, USP13 is phosphorylated by ATM following DNA damage which, in turn, facilitates its DSB localization."

sparser
"Together, these results suggest that USP13 phosphorylation by ATM is important for its recruitment to DSBs, DDR and chemo sensitivity."

sparser
"This result suggests that USP13 may be phosphorylated by ATM following DNA damage."
ATM phosphorylates USP13 on T196. 4 / 4
| 3 1

reach
"Recently, researchers from Mayo Clinic uncover that ATM is activated in response to IR damage, and then phosphorylates USP13 at Thr196 site to stabilize RAP80, leading to the efficient recruitment of BRCA1 to the damage sites 17."

reach
"Recently, some scientists have reported that ATM is activated to phosphorylate USP13 at Thr196 site in response to DNA damage, stabilizing RAP80 to assemble BRCA1 to repair DSBs 17."

reach
"In addition, USP13 was phosphorylated on Thr196 following DNA damage in ATM-proficient cells but not ATM deficient cells (XREF_FIG), suggesting ATM phosphorylates USP13 on T196 following DNA damage."

sparser
"Mechanistically, USP13 is phosphorylated at Thr196 by ATM following DNA damage, which facilitates the localization of USP13 at the double-strand breaks."
VCP affects USP13
2 | 5 9
2 | 5 5

sparser
"By contrast, several other factors enhanced the p97-USP13 interaction."

No evidence text available

sparser
"The data also suggest that the interaction of USP13 with p97 is mediated by several cellular factors with gp78 being a major one."

sparser
"Secondly, depletion of gp78 by shRNA-mediated gene knockdown or gene deletion reduced the USP13-p97 interaction by threefold to fourfold ( xref , xref )."

sparser
"We used the GST pull-down assay to screen a collection of known p97 interactors in order to identify factor(s) that promotes the USP13-p97 interaction."

reach
"USP13 interacts with p97 and VCP, Ufd1, Npl4, and UBXD8 [XREF_BIBR]."

reach
"By contrast, neither GST-USP13 nor GST-USP5 bound p97 (XREF_FIG)."

No evidence text available

reach
"In addition, USP13 interacts with the P97 and valosin containing protein complex, a key chaperone in ER associated degradation, which increases the protein level of CD3delta, an ER associated degradation substrate."

reach
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
GST binds USP13 and VCP. 1 / 1
| 1

sparser
"We expressed these proteins in cells individually and examined the binding of endogenous p97 to GST-USP13."
GST binds USP13, USP5, and VCP. 1 / 1
| 1

sparser
"By contrast, neither GST-USP13 nor GST-USP5 bound p97 ( xref )."
USP13 binds VCP and AMFR. 1 / 1
| 1

sparser
"These results demonstrated a specific and direct interaction between USP13 and gp78 that facilitates USP13-p97 association."
| 1

sparser
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
USP13 affects ACLY
1 | 10 2
USP13 activates ACLY.
| 3
USP13 activates ACLY. 3 / 6
| 3

reach
"USP13-KD also significantly decreased ACLY activity, while USP13 overexpression enhanced ACLY activity in the ACLY activity assay (XREF_SUPPLEMENTARY)."

reach
"USP13 upregulates ACLY and OGDH, two key regulators that drive glutaminolysis, ATP generation and lipid synthesis in cancer metabolism."

reach
"Next, we examined whether USP13 augments the stability of ACLY and OGDH."
USP13 deubiquitinates ACLY.
1 | 4
USP13 deubiquitinates ACLY. 4 / 4
1 | 3

reach
"Taken together, these studies suggest that USP14 is involved in the occurrence and progression of multiple malignant tumors.Other USP family members: USP13, the main regulator of ovarian cancer energy metabolism, specifically deubiquitinates and stabilizes oxoglutarate dehydrogenase and ATP citrate lyase, which can catalyze fatty acid synthesis, glutaminolysis and mitochondrial respiration."

"USP13, the main regulator of ovarian cancer energy metabolism, specifically deubiquitinates and stabilizes oxoglutarate dehydrogenase and ATP citrate lyase, which can catalyze fatty acid synthesis, glutaminolysis and mitochondrial respiration."

reach
"Furthermore, USP13 can deubiquitinate and stabilise ACLY and OGDH and c-Myc in ovarian cancer and glioblastoma, respectively, thereby functioning as an oncogene [XREF_BIBR, XREF_BIBR]."

reach
"USP13 directly deubiquitinates ACLY and OGDH."
USP13-C345A leads to the deubiquitination of ACLY. 1 / 1
| 1

reach
"The C345A mutant of USP13 failed to reduce the ubiquitination of ACLY (XREF_FIG), suggesting that the deubiquitination activity is essential for the function of USP13."
USP13 inhibits ACLY.
| 2
USP13 inhibits ACLY. 2 / 2
| 2

reach
"These data suggest that USP13-KD induces mitochondrial dysfunction and lipogenic dysfunction by reducing OGDH and ACLY activity, thereby reducing glutamine 's reductive carboxylation and glucose 's oxidation for lipid synthesis (XREF_FIG)."

reach
"Depletion of USP13 markedly decreased protein stability of ACLY and OGDH (XREF_FIG; XREF_SUPPLEMENTARY), but had no effect on the transcription of ACLY and OGDH (XREF_FIG)."
USP13 binds ACLY.
| 2
ACLY binds USP13 and OGDH. 2 / 2
| 2

sparser
"Reciprocal analyses in which immunoprecipitated ACLY or OGDH were probed for USP13 confirmed the USP13ACLY and USP13OGDH interactions ( xref )."

sparser
"Western blot analyses of HEK293T cell lysates immunoprecipitated with USP13 antibody showed the interaction of USP13 with ACLY and OGDH ( xref )."
USP13 increases the amount of ACLY.
| 1
USP13-C345A increases the amount of ACLY. 1 / 1
| 1

reach
"We found that the C345A mutant of USP13 was unable to increase the steady-state levels of ACLY and OGDH in contrast to the wild-type USP13 (XREF_FIG)."
USP10 affects USP13
| 4 7
| 4 3

sparser
"USP13 also interacts with USP10, and the interaction increases USP13 deubiquitinating activity ( xref )."

reach
"Although no structural information for USP13 and USP10 is currently available, the enhanced DUB activity when USP13 interacts with USP10 or when they interact with Beclin1 suggest that the interaction of USP13 and USP10 with each other or with their substrates can lead to changes in their conformation which may be critical for the catalytic activities."

reach
"Using spautin-1 as a tool, we explored the interaction of USP10 and USP13 with Vps34 complexes."

reach
"USP13 also interacts with USP10, and the interaction increases USP13 deubiquitinating activity."

sparser
"Although no structural information for USP13 and USP10 is currently available, the enhanced DUB activity when USP13 interacts with USP10 or when they interact with Beclin1 suggest that the interaction of USP13 and USP10 with each other or with their substrates can lead to changes in their conformation which may be critical for the catalytic activities."

reach
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."

sparser
"No orthogonal biophysical methods, such as the surface plasmon response (SPR) or isothermal titration calorimetry (ITC), were used to validate the binding of spautin-1 with USP10 or USP13."
| 2

sparser
"To determine the mechanism by which Vps34 complexes regulate the stability of USP13 and USP10, we examined the possibility that Beclin1 may interact with USP10 and USP13."

sparser
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."
SKP2 binds USP10 and USP13. 1 / 1
| 1

sparser
"The present study uncovers that USP10 is another critical DUB that deubiquitinates SKP2 and suppresses the ubiquitin-dependent proteasomal degradation of SKP2, which is compellingly supported by multiple lines of evidence: (1) our Co-IP experiments revealed compelling interaction of endogenous SKP2 with not only USP13 but also USP10 in KBM5-T315I and KBM5 cells (Fig.  xref ); (2) the interaction of overexpressed SKP2 with overexpressed USP13 or USP10 was also confirmed in HEK293T cells but the USP10 missing the N-terminal 205 amino acid residues failed to bind to SKP2 (Fig.  xref ); (3) overexpression of either USP13 or USP10 led to a higher SKP2 protein level and decreased p27 but overexpression of a DUB activity-disabled USP10 failed to do so (Fig.  xref ); (4) shRNA-mediated knockdown or pharmacological inhibition of USP10 with Spautin1 remarkable decreased SKP2 and phosphorylated Bcr-Abl (but not total Bcr-Abl) but increased p27 in multiple CML cell lines (Fig.  xref , Fig.  xref ); (5) CHX chase assays showed that shortened the halflife of SKP2 protein (Fig.  xref ); and (6) both pharmacological and genetic inhibition of USP10 could markedly increase K48-linked ubiquitinated SKP2 (Fig.  xref )."
| 1

sparser
"We also used an in vitro assay to test the effect of treating HA-Ub-conjugated FLAG-LC3B with recombinant His6-tagged USP10 (His6-USP10) or His6-tagged USP13 (His6-USP13) (specificity control)."
CLK3 affects USP13
| 6 8
CLK3 phosphorylates USP13.
| 5 7
CLK3 phosphorylates USP13 on Y708. 10 / 11
| 4 7

reach
"CLK3 directly interacts with and phosphorylates USP13 at Y708."

sparser
"Moreover, a recent study also found that CDC-like kinase 3 (CLK3) or the cholangiocarcinoma-associated CLK3-Q607R mutant can directly phosphorylate USP13 at Tyr708, and promote its binding to c-Myc ( xref ; xref ) ( xref )."

sparser
"We then examined the physiological significance of USP13 phosphorylation by CLK3 at Y708. xref indicates that CLK3 knockdown in HuCCT1 cells strongly impaired the interaction of USP13 with c-Myc."

sparser
"Notably, the CCA-associated CLK3-Q607R mutant induced USP13-Y708 phosphorylation and enhanced the activity of c-Myc."

sparser
"CLK3 directly phosphorylated USP13 at Y708, which promoted its binding to c-Myc, thereby preventing Fbxl14-mediated c-Myc ubiquitination and activating the transcription of purine metabolic genes."

sparser
"Therefore, we propose that CLK3-induced Y708 phosphorylation of USP13 promotes CCA progression by activating c-Myc–mediated purine synthesis."

reach
"CLK3 directly phosphorylated USP13 at Y708, which promoted its binding to c-Myc, thereby preventing Fbxl14 mediated c-Myc ubiquitination and activating the transcription of purine metabolic genes."

sparser
"CLK3-dependent phosphorylation of USP13 at Y708 promotes CCA progression by activating c-Myc–mediated purine synthesis."

sparser
"We demonstrated that (1) a recurrent Q607R somatic substitution in CLK3 was identified in 8% of 100 human CCAs, particularly in patients with CCA metastasis; (2) the expression of CLK3 was significantly up-regulated in CCA compared with matched control tissues; (3) CLK3 knockdown significantly inhibited CCA aggressiveness in vitro and in vivo; (4) gene ontology term enrichment and MS assays indicated that high CLK3 expression in CCA patients mainly regulated nucleotide metabolism, especially purine biosynthesis; (5) mechanistically, CLK3 directly phosphorylated USP13 at Y708, which promoted its binding to c-Myc, a critical purine synthesis–associated transcription factor, thereby preventing Fbxl14-mediated c-Myc ubiquitination and activating the transcription of purine metabolic genes; (6) the CCA-associated CLK3-Q607R mutant induced USP13-Y708 phosphorylation and enhanced the activity of c-Myc; (7) in turn, c-Myc transcriptionally up-regulated CLK3; (8) importantly, levels of CLK3 significantly correlated with the expression of phospho-USP13-Y708, c-Myc, and ATIC in human CCA specimens; and (9) tacrine hydrochloride was identified as a potential compound to inhibit the aberrant CLK3-enhanced CCA invasiveness."

reach
"CLK3 dependent phosphorylation of USP13 at Y708 promotes CCA progression by activating c-Myc-mediated purine synthesis."
CLK3 phosphorylates USP13. 1 / 1
| 1

reach
"An in vitro kinase assay indicated that WT-CLK3 but not CLK3-K186M was able to directly phosphorylate USP13 (XREF_FIG)."
CLK3 binds USP13.
| 1 1
| 1 1

reach
"A pulldown experiment suggests a direct binding of USP13 to CLK3 (XREF_FIG)."

sparser
"A pulldown experiment suggests a direct binding of USP13 to CLK3 ( xref )."
USP13 affects SIAH2
2 | 7 1
USP13 activates SIAH2.
| 5
USP13 activates SIAH2. 5 / 8
| 5

reach
"Overexpression of USP13 increases Siah2 stability by attenuating its autodegradation."

reach
"Different forms of stress modulate these activities : hypoxia reduces USP13 expression enabling increased Siah2 activity, while cellular stress induces p38 activity and alters Siah localization, affec[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Conversely, inhibition of USP13 expression with corresponding shRNA decreases the stability of both Siah2 and its substrate Spry2."

reach
"Different forms of stress modulate these activities : hypoxia reduces USP13 expression enabling increased Siah2 activity, while cellular stress induces p38 activity and alters Siah localization, affecting its recognition and targeting of substrates 174."

reach
"In melanoma cells, USP13 attenuates the autodegradation of Siah2, a RING (really interesting new gene) finger family E3 ubiquitin ligase."
USP13 binds SIAH2.
2 | 1
2 | 1

sparser
"Overall, this study identifies a new layer of Siah2 regulation mediated by USP13 binding to ubiquitinated Siah2 protein with a concomitant inhibitory effect on its activity under normoxia."

No evidence text available

No evidence text available
USP13 deubiquitinates SIAH2.
| 2
USP13 deubiquitinates SIAH2. 2 / 2
| 2

reach
"The DUB USP13 regulates Siah2 availability and activity as deubiquitination of Siah2 by USP13 results in a more stable, albeit less active, ubiquitin ligase."

reach
"Besides phosphorylation, Siah2 activity can be modulated by the deubiquitinating enzyme USP13, which binds to and deubiquitinates Siah2, increasing its stability but diminishing its activity toward its substrates."
| 2 11
| 2 6

eidos
"It is also noteworthy to point out that USP13 amplifications did not seem to increase B-cell infiltration in LUSC , which has the opposite effect on disease outcome [ 38 ] ."
| PMC

reach
"Finally, Snail knockdown significantly blocked USP13-induced SGC-7901 cell migration and invasion."

reach
"Next, USP13 knockdown prominently reduced the proliferation, epithelial-mesenchymal transition (EMT), migration, and invasion of Hep3B and Huh7 cells, while USP13 overexpression enhanced these biological behaviors of HepG2 and LO2 cells."

reach
"USP13 knockdown prominently suppressed MGC-803 cell migration and invasion."

reach
"On the other hand, although USP13 arm-level gains and high amplifications significantly increased lymphocyte infiltration in HNSC, OV and STAD, higher USP13 levels were correlated with shorter survival in ovarian and gastric cancers."
| PMC

eidos
"USP13 knockdown prominently suppressed MGC-803 cell migration and invasion ."

reach
"It is also noteworthy to point out that USP13 amplifications did not seem to increase B-cell infiltration in LUSC, which has the opposite effect on disease outcome [38]."
| PMC

reach
"We found that the effect of USP13 in promoting the migration and invasion capacities of breast cancer cells can at least partly be achieved through its regulation of Twist1, while Twist1 can inhibit the transcriptional activity of USP13."

reach
"USP13 knockdown in BC cells promoted their proliferation, invasion and migration."

reach
"Meanwhile, silencing of USP13 greatly enhanced proliferation, invasion and migration of the tested cells."

reach
"Overexpression of miR-130b/301b or USP13 knockdown by shRNA delivery decreases USP13 expression, and further reduces PTEN protein expression and leads to enhancement of cell proliferation, invasion and migration."

reach
"Results suggested that overexpression of NF-kB p65 greatly increased the number of migrated and invaded cells, however knockdown of miR-130b/301b or restoration of USP13 rescued the increased cell invasion and migration caused by NF-kB activation."

reach
"The in vivo experiments finally confirmed that USP13 dramatically repressed synovial hyperplasia, inflammatory cell infiltration, cartilage damage and bone loss in collagen induced arthritis (CIA) mice via the same molecular mechanisms detected in vitro."
Spautin-1 affects USP13
| 2 8
Spautin-1 inhibits USP13.
| 2 4
Spautin-1 inhibits USP13. 4 / 4
| 4

reach
"Another autophagy inhibitor, spautin-1, degrades class III PI3K by inhibiting the activity of the ubiquitin specific peptidases, USP10 and USP13 (Mateo et al., 2013, Liu et al., 2011)."

reach
"To directly test if spautin-1 can inhibit the deubiquitinating activity of USP10 and USP13, we tested the activity of isolated USP10 and USP13 on ubiquitinated Beclin1 in vitro."

reach
"Further, inhibition of USP13 by spautin-1 caused a decrease in TXNIP levels in MLE12 cells (Fig. 4)."

reach
"Using this assay, we found that spautin-1 inhibited USP10 and USP13 with IC 50 of ~ 0.6-0.7 microM while having no inhibitory activity toward CYLD which is also a member of ubiquitin specific peptidase family (XREF_FIG)."
| 2

eidos
"Further , inhibition of USP13 by spautin-1 caused a decrease in TXNIP levels in MLE12 cells ( Fig. 4 ) ."

eidos
"Spautin-1 was shown to inhibit USP-13 , a de-ubiquitinase of the host cell hijacked by the SARS-CoV-2 which led to successful ubiquitination of nsp-13 [ 109 ] ."
Spautin-1 decreases the amount of USP13.
| 2
Spautin-1 decreases the amount of USP13. 2 / 2
| 2

reach
"Interestingly, the treatment of spautin-1 also reduced the levels of USP10 and USP13, but not USP14, a DUB involved in regulating proteasome function, or Rubicon, a negative regulator of type III PI3 kinase (XREF_FIG)."

reach
"In addition, spautin-1 also downregulated the expression of USP10 and USP13 at the protein level [53]."
Spautin-1 activates USP13.
| 2
Spautin-1 activates USP13. 2 / 2
| 2

reach
"Briefly, spautin-1 targets two ubiquitin specific peptidases (USP10 and USP13), both of which mediate deubiquitination of Beclin1 and p53, thus leading to their proteasomal degradation [93]."

reach
"Because the reductions in the levels of USP10 and USP13 in H4-LC3-GFP cells treated with spautin-1 appeared later than the reductions in the levels of Vps34 complexes and autophagy (XREF_FIG), the reduced levels of USP10 and USP13 are unlikely to be the primary reason for the ability of spautin-1 to reduce the levels of PtdIns3P and inhibit autophagy."
| 5 5
USP13 increases the amount of extracellular matrix.
| 5
USP13 increases the amount of extracellular matrix. 5 / 5
| 5

reach
"The results indicate that USP13 promotes ECM expression by stabilizing Smad4 in lung fibroblasts and plays a role in the maintenance of the extracellular matrix in lungs."

reach
"The reductions in both protein levels and mRNA expression of ECM were observed in the isolated lung fibroblasts from USP13 deficient mice, suggesting that downregulation of USP13 reduces ECM levels through inhibiting its transcription."

reach
"In summary, USP13 is a positive regulator of Smad4 stability and promotes ECM expression in lung fibroblast cells."

reach
"In this study, we identify that USP13 promotes ECM expression by stabilizing Smad4 in lung fibroblast cells."

reach
"To investigate the molecular mechanisms by which USP13 modulates ECM expression, we focused on the role of USP13 on Smad4 expression."
| 5

eidos
"To investigate the molecular mechanisms by which USP13 modulates ECM expression , we focused on the role of USP13 on Smad4 expression ."

eidos
"The results indicate that USP13 promotes ECM expression by stabilizing Smad4 in lung fibroblasts and plays a role in the maintenance of the extracellular matrix in lungs ."

eidos
"We used a mild CS exposure system and found that USP13 deficiency caused loss of extracellular matrix , increased lung inflammation and enlargement of alveolar spaces in response to CS exposure ."

eidos
"28 In this study , we identify that USP13 promotes ECM expression by stabilizing Smad4 in lung fibroblast cells ."

eidos
"Taken together , USP13 deficiency causes loss in extracellular matrix levels in lungs , thereby enlarging alveolar space in response to CS exposure ."
USP13 affects autophagy
| 3 7
USP13 activates autophagy.
| 3 3
| 3 3

eidos
"Our data showed that USP13 knockdown inhibited autophagy , which is consistent with the previous report that USP13 promotes autophagy through deubiquitinating BECN1 to stabilize the protein ( Liu et al ., 2011 ) ."

eidos
"Therefore , depletion of USP13 promotes cellular apoptosis and impedes autophagy ."

reach
"Collectively, our study results indicate that USP13 promotes glycolysis and tumor progression in OS by stabilizing METTL3, thereby stabilizing ATG5 mRNA and facilitating autophagy in OS."

reach
"USP13 stabilizes the Vps34 complex to promote autophagy, and therefore, inhibition of USP13 reduces autophagy."

reach
"Spautin-1 inhibits autophagy by reducing the activity of USP10 and USP13, which promotes a major aspect of autophagy: degradation by Vps34 complexes."

eidos
"USP13 stabilizes the Vps34 complex to promote autophagy , and therefore , inhibition of USP13 reduces autophagy ."
USP13 inhibits autophagy.
| 4
| 4

reach
"Because the reductions in the levels of USP10 and USP13 in H4-LC3-GFP cells treated with spautin-1 appeared later than the reductions in the levels of Vps34 complexes and autophagy (XREF_FIG), the reduced levels of USP10 and USP13 are unlikely to be the primary reason for the ability of spautin-1 to reduce the levels of PtdIns3P and inhibit autophagy."

reach
"The function of USP10 and USP13 is reversed by a chemical compound, spautin-1, which inhibits autophagy by promoting Beclin-1 degradation."

reach
"As expected, USP13 overexpression inhibited autophagy and survival of PC12 cells under OGD/R conditions, and Spautin‐1 treatment counteracted these effects."

reach
"Interestingly, Spautin-1-mediated specific and potent inhibition of deubiquitinating activity of USP10 and USP13 was shown to promote proteasomal degradation of PIK3C3 and thereby inhibit autophagy [179]."
USP13 affects UBL4A
4 2 | 1 2
USP13 binds UBL4A.
4 | 1
4 | 1

sparser
"Additionally, as catalytic-inactive DUBs often trap their substrates, can a catalytic-inactive USP13 bind preferentially to Ubl4A?"

No evidence text available

No evidence text available

No evidence text available

No evidence text available
USP13 ubiquitinates UBL4A.
| 1 1
USP13 ubiquitinates UBL4A. 2 / 3
| 1 1

reach
"USP13 and gp78 control ubiquitination of Ubl4A."

sparser
"USP13 and gp78 control ubiquitination of Ubl4A."
USP13 deubiquitinates UBL4A.
2 |
USP13 deubiquitinates UBL4A. 2 / 2
2 |

"we identify USP13 as a gp78-associated DUB that eliminates ubiquitin conjugates from Ubl4A to maintain the functionality of Bag6."

No evidence text available
EGFR affects USP13
| 4 6
EGFR binds USP13.
| 2 6
| 2 6

sparser
"The interaction of wild‐type EGFR with USP13 was hardly detectable, whereas USP13 coimmunoprecipitated with the EGFR ΔE746‐A750 and L858R mutants (Figure xref ), indicating that USP13 associates much more tightly with mutant than wild‐type EGFR."

sparser
"We then recapitulated the interaction between exogenous USP13 and EGFR in HEK293T cells."

sparser
"The fraction of mutant EGFR bound to USP13 was also enriched in those two types of ubiquitin chains (Figure xref )."

reach
"We found that USP13 inhibits mutant EGFR degradation by a process that leads to the accumulation of ubiquitinated (K48 and K63) EGFR, which is bound to c-Cbl and USP13."

sparser
"The association is dependent on the presence of c‐Cbl, as we observed that increased expression of c‐Cbl enhanced USP13‐mutant EGFR interaction."

sparser
"The isopeptidase‐independent mechanism of action proposed so far for USP13 relies on its UBA domains, which bind to ubiquitinated substrates, possibly inducing structural changes that stabilize them. xref Coincidentally, K63‐linked ubiquitin chains are required for EGFR sorting and degradation. xref This type of chains has also been reported as the preferred substrates for the USP13 UBA domains. xref We found that the UBA domains of USP13 were required for the binding to and stabilizing EGFR, as mutated UBA domains in USP13 resulted in a loss of USP13EGFR interaction and of EGFR stabilization."

reach
"We then recapitulated the interaction between exogenous USP13 and EGFR in HEK293T cells."

sparser
"By employing the USP13‐C345A mutant or versions mutated in UBA1, UBA2 or both, we established that the UBA domains' integrity is necessary for USP13 to bind to mutant EGFR (Figure xref )."
EGFR increases the amount of USP13.
| 1
EGFR increases the amount of USP13. 1 / 1
| 1

reach
"On the other hand, we found that EGFR inhibition downregulated the endogenous levels of USP13."
EGFR activates USP13.
| 1
EGFR activates USP13. 1 / 1
| 1

reach
"In addition, the combined effect of USP13 downregulation by EGFR inhibition and USP13 siRNA also increased the negative effect on EGFR stability."
UIMC1 affects USP13
| 7 2
UIMC1 binds USP13.
| 6
| 6

reach
"USP13 interacts with and deubiquitinates RAP80."

reach
"Consistent with their reports, we also found that RAP80 binded with USP13 in EC cells treated with or without cisplatin."

reach
"Also, USP13 interacts to and deubiquitylates receptor-associated protein 80 (RAP80) following DNA damage to activate its ability, promoting DNA-damage responses ."

reach
"To evaluate the recruitment of USP13 and RAP80 at DSBs, we analysed the kinetics of GFP-USP13 and GFP-RAP80 accumulation on stripes after laser microirradiation."

reach
"Also, USP13 interacts with and deubiquitylates RAP80 to activate its function ."

reach
"To test our hypothesis, we first examined whether USP13 interacts with RAP80."
UIMC1 ubiquitinates USP13.
| 2
UIMC1 ubiquitinates USP13. 2 / 2
| 2

sparser
"We found that mutating the 3 sites (K75, K90 and K112, 3KR) near or at the UIM domains, but not the other sites, abolished USP13 deficiency-induced RAP80 ubiquitination ( xref and xref )."

sparser
"In addition, we found all the single mutations of these three sites cannot abolish the USP13 deficiency-induced RAP80 ubiquitination ( xref )."
UIMC1 phosphorylates USP13.
| 1
Ubiquitinated UIMC1 leads to the phosphorylation of USP13. 1 / 1
| 1

reach
"Furthermore, reconstitution of USP13 WT but not the T196A fully reversed the increase in RAP80 ubiquitination induced by USP13 deficiency (XREF_SUPPLEMENTARY), suggesting USP13 phosphorylation by ATM is important for RAP80 deubiquitination."
JUN affects USP13
9 |
JUN decreases the amount of USP13. 9 / 9
9 |

No evidence text available

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reach
"Furthermore, the deubiquitinating enzyme USP13 stabilizes the anti-inflammatory receptor IL-1R8/Sigirr to suppress lung inflammation [40,41,42]."

reach
"Further studies showed that USP13 reduced LPS-induced oxidative stress and inflammation by inducing Nrf2."

reach
"The deubiquitinase USP13 stabilizes the anti-inflammatory receptor IL-1R8 and Sigirr to suppress lung inflammation."

reach
"USP13 reduces septic mediated cardiomyocyte oxidative stress and inflammation by inducing Nrf2."

reach
"We then showed that over-expressing USP13 markedly suppressed inflammatory response, oxidative stress and apoptosis in H-FLSs upon IL-1beta or TNF-alpha challenge, whereas USP13 knockdown exhibited detrimental effects."

reach
"We have shown that USP13 deficiency promotes lung inflammation."
| 2

reach
"Hepatocyte-specific loss of the Usp13 disrupts liver metabolic homeostasis, followed by glycometabolic disorder, lipid deposition, increased inflammation, and markedly promotes NASH development."

reach
"The roles of USP13 and the newly synthesized protein in liver inflammation, injury, and repair will be the future focuses."
| 6

reach
"In this study, we identified USP13 as the first deubiquitinase that reverses PTEN poly-ubiquitination and stabilizes PTEN protein, and found that USP13 suppresses tumorigenesis and glycolysis through PTEN."

reach
"Indeed, knockdown of USP13 increased glucose uptake and glycolysis, which could be fully reversed by restoration of PTEN, as gauged by lactate production and glucose incorporation assays (XREF_FIG)."

reach
"miR-135b promoted cell proliferation and glycolysis that could be reversed by the overexpression of USP13 or PTEN."

reach
"Conversely, overexpression of USP13 suppresses tumorigenesis and glycolysis in PTEN positive but not PTEN-null breast cancer cells."
Modified USP13 inhibits glycolytic process. 2 / 2
| 2

reach
"Loss of USP13 in breast cancer cells promotes AKT phosphorylation, cell proliferation, anchorage independent growth, glycolysis and tumor growth through downregulation of PTEN."

reach
"Loss of USP13 in breast cancer cells promotes AKT phosphorylation, cell proliferation, anchorage independent growth, glycolysis and tumour growth through downregulation of PTEN."
| 2

reach
"USP13 was found to upregulate in osteosarcoma (OS) specimens and promote OS progression through regulating aerobic glycolysis."

reach
"Collectively, our study results indicate that USP13 promotes glycolysis and tumor progression in OS by stabilizing METTL3, thereby stabilizing ATG5 mRNA and facilitating autophagy in OS."
| 2 6

eidos
"USP13 suppression induces apoptosis upon EGFR inhibition of mutant NSCLC cells USP13 downregulation or inhibition severely compromised the viability of PC9 and HCC827 cells when cotreated with afatinib ."

reach
"In addition, terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling (TUNEL) assay demonstrated that expression of the mutant c-Myc diminished the increased apoptosis caused by USP13 disruption in GSC derived xenografts (not depicted)."

reach
"In conclusion, this study demonstrated that USP13 was downregulated in septic myocardial injury tissues, and USP13 overexpression increased Nrf2 levels and reduced apoptosis."

reach
"We then showed that over-expressing USP13 markedly suppressed inflammatory response, oxidative stress and apoptosis in H-FLSs upon IL-1beta or TNF-alpha challenge, whereas USP13 knockdown exhibited detrimental effects."

reach
"3.3 USP13 suppression induces apoptosis upon EGFR inhibition of mutant NSCLC cells."

reach
"However, in the context of hypoxic or oxidative stresses, USP13 loss caused a substantial decrease of cell viability and a significant increase of cell apoptosis in both TOV-21G and SW-1573 tumor models."

reach
"Additionally, it is found that USP13 abrogates cell apoptosis by preventing excessive ubiquitin-proteasome degradation of Bcl-XL."

eidos
"Results Depletion of USP13 Induces Apoptosis and Suppresses Autophagy Impaired ERAD pathway causes prolonged ER stress , which activates alternative signal pathways and promotes apoptosis ( Hetz , 2012 ; Hetz and Papa , 2018 ; Tabas and Ron , 2011 ) ."
USP13 affects STAT1
2 | 4 2
USP13 deubiquitinates STAT1.
| 4
USP13 deubiquitinates STAT1. 4 / 4
| 4

reach
"Congruently, STAT1 ubiquitination is reduced in cells by USP13 overexpression and increased with USP13 knockdown regardless of IFNa treatment (115) ."

reach
"Congruently, STAT1 ubiquitination is reduced in cells by USP13 overexpression and increased with USP13 knockdown regardless of IFNα treatment (115)."

reach
"A recent report suggested that the deubiquitinase USP13 decreased STAT1 ubiquitination, which however was independent of IFNs treatment [XREF_BIBR]."

reach
"More specifically, USP13 positively regulates the antiviral activity of IFNa against DEN-2 virus replication by deubiquitinating and stabilizing STAT1 (115) ."
USP13 binds STAT1.
2 | 2
2 | 1

No evidence text available

No evidence text available

sparser
"Usp13 mainly interacted with the nonactivated form of Stat1 ( xref )."
| 1

sparser
"For example, the three deubiquitinating enzymes BRCC36, USP13, and USP39 interact with STAT1 and decrease the K63-, K48- and K6-linked polyubiquitin chains of STAT1 respectively (110, 115, 118)."
USP13 affects SIGIRR
| 5 3
USP13 binds SIGIRR.
| 2 3
| 2 3

sparser
"USP13 is associated with Sigirr."

sparser
"Co-immunoprecipitation (Co-IP) also revealed USP13's association with Sigirr ( xref b, c)."

reach
"To determine molecular mechanisms by which GSK3β promotes Sigirr degradation, we examined the effect of GSK3β-mediated phosphorylation on the association between Sigirr and USP13."

sparser
"Overexpression of GSK3β reduced the association between Sigirr and USP13 ( xref ), suggesting that GSK3β-mediated phosphorylation prevents Sigirr degradation from Sigirr interaction with its stabilizer, USP13."

reach
"Overexpression of GSK3β reduced the association between Sigirr and USP13 (Fig. 7B), suggesting that GSK3β-mediated phosphorylation prevents Sigirr degradation from Sigirr interaction with its stabilizer, USP13."
USP13 deubiquitinates SIGIRR.
| 2
USP13 deubiquitinates SIGIRR. 2 / 2
| 2

reach
"We have shown that deubiquitination of Sigirr by USP13 increased Sigirr half-life (L. Li et al., 2019)."

reach
"The ubiquitination and degradation of Sigirr is negatively regulated by a deubiquitinase, USP13 (L. Li et al., 2019)."
USP13 ubiquitinates SIGIRR.
| 1
USP13 leads to the ubiquitination of SIGIRR. 1 / 1
| 1

reach
"Knockdown of USP13 in cells increased IL-1R8 and Sigirr poly-ubiquitination and reduced its stability, which enhanced LPS induced TLR4 signaling and cytokine release."
USP13 affects AKT
| 1 6
USP13 dephosphorylates AKT.
| 3
Modified USP13 leads to the dephosphorylation of AKT. 3 / 3
| 3

reach
"This overexpression upregulated PTEN protein and downregulated AKT and FOXO1/3 phosphorylation in the MDAMB-231 cell line (XREF_FIG) which expresses moderate but detectable levels of endogenous PTEN (XREF_SUPPLEMENTARY), while knockdown of PTEN in USP13 overexpressing MDA-MB-231 cells rescued the phosphorylation of both AKT and FOXO1/3 (XREF_SUPPLEMENTARY)."

reach
"Loss of USP13 in breast cancer cells promotes AKT phosphorylation, cell proliferation, anchorage independent growth, glycolysis and tumor growth through downregulation of PTEN."

reach
"Loss of USP13 in breast cancer cells promotes AKT phosphorylation, cell proliferation, anchorage independent growth, glycolysis and tumour growth through downregulation of PTEN."
USP13 phosphorylates AKT.
| 2
USP13 leads to the phosphorylation of AKT. 2 / 2
| 2

reach
"Similarly, depletion of USP13 downregulated PTEN protein and upregulated AKT and FOXO1/3 phosphorylation in MCF10A (XREF_FIG) and MCF7 (XREF_FIG) mammary epithelial cells and in HCT116 colon cancer cells (XREF_FIG), but not in the isogenic PTEN-null HCT116 cells (XREF_FIG)."

reach
"Depletion of USP13 in breast cancer cells promoted AKT phosphorylation, cell proliferation, glycolysis, and tumor growth through downregulation of PTEN."
USP13 activates AKT.
| 1
USP13 activates AKT. 1 / 2
| 1

eidos
"Wu et al demonstrate that amplification of USP13 in some NSCLC leads to enhanced AKT and MAPK signaling that sustains cancer progression ."
USP13 inhibits AKT.
| 1
USP13 inhibits AKT. 1 / 1
| 1

reach
"We conclude from these data that USP13 inhibits AKT signaling through positive regulation of PTEN protein."
USP13 affects SMAD4
| 1 6
USP13 activates SMAD4.
| 1 2
USP13 activates SMAD4. 3 / 3
| 1 2

reach
"Knockdown of USP13 augmented Smad4 degradation and reduced Smad4 half-life (Fig 6A)."

eidos
"Knockdown of USP13 augmented Smad4 degradation and reduced Smad4 half-life ( Fig 6A ) ."

reach
"Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells."
USP13 ubiquitinates SMAD4.
| 2
USP13 leads to the ubiquitination of SMAD4. 2 / 2
| 2

reach
"Further, an in vivo ubiquitination assay showed that downregulation of USP13 increased Smad4 polyubiquitination (Fig 6B)."

reach
"Knockdown of USP13 decreased Smad4 half-life and promoted Smad4 ubiquitination."
USP13 deubiquitinates SMAD4.
| 2
USP13 deubiquitinates SMAD4. 2 / 2
| 2

reach
"USP13 stabilizes Smad4 by deubiquitinating Smad4.."

reach
"Knockdown of USP13 decreased Smad4 half-life and promoted Smad4 ubiquitination."
USP13 affects Neoplasms
| 5
USP13 activates Neoplasms.
| 4
| 4

reach
"Finally, miR‐367 silencing also significantly inhibited 3D cell invasion in tumor spheroids of both USP13‐MED and USP7‐ATRT cells (Fig. 4), suggesting that miR‐367 silencing inhibits two key features of tumor aggressiveness, namely, stem‐like properties and invasive behavior."

reach
"Collectively, our study results indicate that USP13 promotes glycolysis and tumor progression in OS by stabilizing METTL3, thereby stabilizing ATG5 mRNA and facilitating autophagy in OS."

reach
"Nude mouse experiments indicated that depletion of USP13 in GC cells dramatically suppressed tumor growth in vivo."

reach
"Notably, USP13 greatly enhanced peritoneal metastasis of ovarian tumors with frequent development of hemorrhagic ascites."
USP13 inhibits Neoplasms.
| 1
| 1

reach
"Targeting USP13 may inhibit tumor growth and provide additional benefits in cooperation with DDR inhibitors and immunotherapy."
USP13 affects FlaG
| 7
| 5

sparser
"Immunoprecipitation with anti-FLAG antibody demonstrated that FLAG-USP13 interacted with over-expressed Mcl-1 (Fig. xref )."

sparser
"We first performed co-immunoprecipitation assays to confirm the interaction between USP13 and Mcl-1 by transfecting HEK293T cells with FLAG-USP13 and V5-hMcl-1."

sparser
"HEK293T cells were transfected with FLAG-USP13, V5-Mcl-1 and HA-Ubiquitin, and anti-V5 immunoprecipitates were probed for the level of ubiquitination using the HA-antibody."

sparser
"We found that endogenous TopBP1 co-immunoprecipitated with FLAG-USP13 ( xref )."

sparser
"We observed that addition of His6-USP10, but not His6-USP13, reduced the amount of HA-Ub-conjugated FLAG-LC3B ( xref , C and D ), consistent with USP10 specifically catalyzing the deubiquitination of LC3B. Finally, we found that GFP-tagged LC3B (GFP-LC3B) co-immunoprecipitated with FLAG-USP10 but not FLAG-USP13 ( xref E )."
USP13 binds MCL1 and FlaG. 1 / 1
| 1

sparser
"Immunoprecipitation with anti-FLAG antibody demonstrated that FLAG-USP13 interacted with over-expressed Mcl-1 (Fig. xref )."
| 1

sparser
"We also used an in vitro assay to test the effect of treating HA-Ub-conjugated FLAG-LC3B with recombinant His6-tagged USP10 (His6-USP10) or His6-tagged USP13 (His6-USP13) (specificity control)."
USP13 affects CDH1
| 1 3 3
USP13 binds CDH1.
| 2 3
| 2 3

sparser
"We performed immunoprecipitation assays followed by western-blotting to assess the interaction between USP13 and CDH1."

sparser
"Shortly after this finding, the interaction of USP13 with CDH1 was also confirmed by the same team ( xref )."

reach
"We performed immunoprecipitation assays followed by western-blotting to assess the interaction between USP13 and CDH1."

reach
"We found that USP13 binds to the APC/C adaptor CDH1."

sparser
"We found that USP13 binds to the APC/C adaptor CDH1."
USP13 activates CDH1.
| 1 1
USP13 activates CDH1. 2 / 2
| 1 1

reach
"USP13 modulates the stability of the APC/C adaptor CDH1."

eidos
"In addition , we report for the first time that USP13 controls CDH1 protein levels in cells : overexpression of USP13 increased CDH1 levels , whereas depletion of USP13 decreased CDH1 levels ."
FlaG affects USP13
| 7
| 5

sparser
"Immunoprecipitation with anti-FLAG antibody demonstrated that FLAG-USP13 interacted with over-expressed Mcl-1 (Fig. xref )."

sparser
"We first performed co-immunoprecipitation assays to confirm the interaction between USP13 and Mcl-1 by transfecting HEK293T cells with FLAG-USP13 and V5-hMcl-1."

sparser
"HEK293T cells were transfected with FLAG-USP13, V5-Mcl-1 and HA-Ubiquitin, and anti-V5 immunoprecipitates were probed for the level of ubiquitination using the HA-antibody."

sparser
"We found that endogenous TopBP1 co-immunoprecipitated with FLAG-USP13 ( xref )."

sparser
"We observed that addition of His6-USP10, but not His6-USP13, reduced the amount of HA-Ub-conjugated FLAG-LC3B ( xref , C and D ), consistent with USP10 specifically catalyzing the deubiquitination of LC3B. Finally, we found that GFP-tagged LC3B (GFP-LC3B) co-immunoprecipitated with FLAG-USP10 but not FLAG-USP13 ( xref E )."
USP13 binds MCL1 and FlaG. 1 / 1
| 1

sparser
"Immunoprecipitation with anti-FLAG antibody demonstrated that FLAG-USP13 interacted with over-expressed Mcl-1 (Fig. xref )."
| 1

sparser
"We also used an in vitro assay to test the effect of treating HA-Ub-conjugated FLAG-LC3B with recombinant His6-tagged USP10 (His6-USP10) or His6-tagged USP13 (His6-USP13) (specificity control)."
USP13 affects nsp13
| 6
USP13 ubiquitinates nsp13.
| 2
USP13 leads to the ubiquitination of nsp13. 2 / 2
| 2

reach
"Loss of USP13 led to the increase of ubiquitinated nsp13."

reach
"Predicting hijacking of host de-ubiquitination by the virus, Guo et al. checked for interactions between host de-ubiquitinase and nsp13 and found USP13 to interact with it and observed that loss of USP13 led to greater ubiquitinated nsp13."
USP13 deubiquitinates nsp13.
| 2
USP13 deubiquitinates nsp13. 2 / 2
| 2

reach
"We found that nsp13 interacts with the deubiquitinase USP13, which deubiquitinates and stabilizes nsp13."

reach
"Loss of USP13 enhances ubiquitination of nsp13 and destabilizes nsp13 protein."
USP13 increases the amount of nsp13.
| 1
USP13 increases the amount of nsp13. 1 / 1
| 1

reach
"Consistently, overexpression of wild type (WT), but not the catalytic-inactive (CA) mutant of USP13 with a mutation at the core enzymatic domain, could rescue the decreased nsp13 levels caused by USP13 depletion, suggesting that USP13 can regulate nsp13 levels most likely by deubiquitinating and consequently stabilizing nsp13."
USP13 binds nsp13.
| 1
| 1

reach
"We found that nsp13 interacts with the deubiquitinase USP13, which deubiquitinates and stabilizes nsp13."
USP13 affects VCL
| 4 2
USP13 increases the amount of VCL.
| 4
USP13 increases the amount of VCL. 4 / 4
| 4

reach
"These results suggest that USP13 upregulates vinculin protein levels, but not the mRNA levels, through an enzymatically mediated mechanism."

reach
"To further validate the upregulation of vinculin protein levels by USP13, western blot analysis and RT-qPCR analysis were performed."

reach
"USP13 upregulates vinculin protein levels, but not mRNA levels."

reach
"Among the identified proteins, the vinculin protein level was upregulated by USP13 through an enzymatically mediated mechanism, which was confirmed by western blot analysis and RT-qPCR."
USP13 binds VCL.
| 2
| 2

sparser
"However, sufficient follow-up validation studies are required in order to determine whether vinculin protein directly interacts with USP13."

sparser
"Among the identified proteins, the altered expression level of vinculin was associated with the DUB activity of USP13, which was confirmed by western blot analysis and reverse transcription-quantitative polymerase chain reaction (RT-qPCR)."
USP13 affects USP13
| 1 4
USP13 activates USP13.
| 1 2
USP13 activates USP13. 3 / 4
| 1 2

reach
"We further demonstrated that Casein kinase 2 (CK2) directly interacts with and phosphorylates USP13 at Thr122, which promotes the stability of USP13 protein."

reach
"Overexpression of USP13 in PC12 cells almost reversed the Spautin‐1‐mediated decrease in NLRP3, USP13, Beclin1 and GSDMD‐N expression levels (Figure 3D,E)."

eidos
"( D ) Hypoxia increased USP13 , TLR4 , MyD88 , and P-NF-kappaB p65 proteins , which was subsequently reduced by USP13 knockdown ."
USP13 decreases the amount of USP13.
| 2
USP13 decreases the amount of USP13. 2 / 2
| 2

reach
"We found that both USP13 shRNAs reduced USP13 expression in vivo compared to the control shRNA although with different efficiencies."

reach
"Moreover, treatment with a proteasome inhibitor blocked the c-Myc loss caused by USP13 knockdown (not depicted), but overexpression of USP13 delayed c-Myc turnover in the presence of cycloheximide (not depicted), suggesting a critical role of USP13 in preventing proteasomal degradation of c-Myc in GSCs."
USP13 affects SNAI1
| 5 1
USP13 activates SNAI1.
| 3
USP13 activates SNAI1. 3 / 3
| 3

reach
"In conclusion, USP13 overexpression was frequently detected in GC and contributed to the EMT and metastasis of GC by stabilizing Snail."

reach
"Deubiquitinase USP13 promotes the epithelial-mesenchymal transition and metastasis in gastric cancer by maintaining Snail protein."

reach
"Mechanistically, USP13 knockdown promoted Snail degradation, which could be blocked by the proteasome inhibitor MG132."
USP13 increases the amount of SNAI1.
| 1
USP13 increases the amount of SNAI1. 1 / 1
| 1

reach
"Interestingly, USP13 remarkably enhanced Snail protein expression but did not affect its mRNA levels in GC cells."
USP13 deubiquitinates SNAI1.
| 1
USP13 deubiquitinates SNAI1. 1 / 1
| 1

reach
"USP13 interacted with Snail to deubiquitinate and stabilize Snail in GC cells."
USP13 binds SNAI1.
| 1
| 1

sparser
"USP13 interacted with Snail to deubiquitinate and stabilize Snail in GC cells."
JNK affects USP13
| 3 3
JNK activates USP13.
| 3 1
JNK activates USP13. 4 / 4
| 3 1

eidos
"Furthermore , among the inhibitors of matrix metalloproteinases ( MMPs ) , ROCK , p38 MAPK , and JNK , only JNK inhibitor II attenuated USP13 degradation ( Fig. 7D ) ."

reach
"Inhibition of c-Jun N-terminal kinase (JNK) attenuates USP13 degradation, indicating that a JNK-dependent new protein synthesis is necessary for USP13 degradation."

eidos
"Inhibition of JNK had no effect on LPS reduction of USP13 in Raw264.7 cells , while JNK inhibitor attenuates USP13 degradation in Kupffer cells ."

eidos
"Inhibition of c-Jun N-terminal kinase ( JNK ) attenuates USP13 degradation , indicating that a JNK-dependent new protein synthesis is necessary for USP13 degradation ."
JNK inhibits USP13.
| 1
JNK inhibits USP13. 1 / 1
| 1

reach
"Inhibition of c-Jun N-terminal kinase (JNK) attenuates USP13 degradation, indicating that a JNK-dependent new protein synthesis is necessary for USP13 degradation."
JNK increases the amount of USP13.
| 1
JNK increases the amount of USP13. 1 / 1
| 1

reach
"JNK inhibition also decreased USP13 mRNA expression, demonstrating that JNK mediated c-Jun activity may regulate USP13 at the transcriptional level."
USP13 affects glucose
| 5
USP13 inhibits glucose.
| 3
| 3

reach
"Indeed, knockdown of USP13 increased glucose uptake and glycolysis, which could be fully reversed by restoration of PTEN, as gauged by lactate production and glucose incorporation assays (XREF_FIG)."

reach
"Therefore, USP13-KD also decreases glucose oxidation into TCA cycle metabolites."

reach
"Functional studies demonstrated that overexpression of USP13 suppressed OSCC cell proliferation, glucose uptake and lactate production in vitro and inhibited tumor growth in vivo."
USP13 increases the amount of glucose.
| 1
Modified USP13 increases the amount of glucose. 1 / 1
| 1

reach
"Furthermore, USP13 overexpression induced phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression and repressed the activation of AKT as well as the expression of the downstream effectors glucose transporter-1 (GLUT1) and hexokinase-2 (HK2)."
USP13 decreases the amount of glucose.
| 1
Modified USP13 decreases the amount of glucose. 1 / 1
| 1

reach
"Furthermore, USP13 overexpression induced phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression and repressed the activation of AKT as well as the expression of the downstream effectors glucose transporter-1 (GLUT1) and hexokinase-2 (HK2)."
USP13 affects cell
| 5
USP13 activates cell. 5 / 5
| 5

eidos
"Notably , USP13 knockdown repressed hypoxia-induced activation of the TLR4 / MyD88 / NF-kappaB pathway in HCC cells ."

eidos
"Significantly , USP13 knockdown inhibited hypoxia-induced activation of the TLR4 / MyD88 / NF-kappaB pathway in HCC cells ."

eidos
"Interestingly , the USP13 knockdown repressed the hypoxia-induced activation of the TLR4 / MyD88 / NF-kappaB pathway in HCC cells ( Figure 7D ) ."

eidos
"USP13 knockdown inhibits the proliferation of HCC cells ."

eidos
"Conversely , suppression of USP13 led to GC cell cycle arrest in G1 phase and the inhibition of cell proliferation ."
USP13 affects SKP2
1 | 2 2
USP13 binds SKP2.
1 | 1 2
1 | 1 1

reach
"We found that SKP2 interacted with USP10, USP13, UCHL5, USP14, and USP7 in KBM5-T315I cells."

No evidence text available

sparser
"UFD1 was reported to function as a scaffold for the USP13-Skp2 (the F-box adaptor of the E3 ubiquitin ligase SCF Skp2 ) interaction, maintaining Skp2 stability."
SKP2 binds USP10 and USP13. 1 / 1
| 1

sparser
"The present study uncovers that USP10 is another critical DUB that deubiquitinates SKP2 and suppresses the ubiquitin-dependent proteasomal degradation of SKP2, which is compellingly supported by multiple lines of evidence: (1) our Co-IP experiments revealed compelling interaction of endogenous SKP2 with not only USP13 but also USP10 in KBM5-T315I and KBM5 cells (Fig.  xref ); (2) the interaction of overexpressed SKP2 with overexpressed USP13 or USP10 was also confirmed in HEK293T cells but the USP10 missing the N-terminal 205 amino acid residues failed to bind to SKP2 (Fig.  xref ); (3) overexpression of either USP13 or USP10 led to a higher SKP2 protein level and decreased p27 but overexpression of a DUB activity-disabled USP10 failed to do so (Fig.  xref ); (4) shRNA-mediated knockdown or pharmacological inhibition of USP10 with Spautin1 remarkable decreased SKP2 and phosphorylated Bcr-Abl (but not total Bcr-Abl) but increased p27 in multiple CML cell lines (Fig.  xref , Fig.  xref ); (5) CHX chase assays showed that shortened the halflife of SKP2 protein (Fig.  xref ); and (6) both pharmacological and genetic inhibition of USP10 could markedly increase K48-linked ubiquitinated SKP2 (Fig.  xref )."
USP13 increases the amount of SKP2.
| 1
USP13 increases the amount of SKP2. 1 / 1
| 1

reach
"XREF_FIG, only the knockdown of USP10 and USP13 decreased the expression of SKP2."
USP13 affects PXN
| 5
| 5

sparser
"USP13 interacts with Paxillin and NLRP3, phosphorylation of Paxillin at Y31 is important for Paxillin-USP13 interaction, and USP13 is essential for Paxillin-mediated NLRP3 deubiquitination upon ATP treatment."

sparser
"Moreover, the domains of USP13 required for USP13-NLRP3 interaction and USP13-Paxillin association were assessed by constructing and analyzing plasmids encoding for WT USP13(1–863) and four truncated proteins (Fig.  xref i)."

sparser
"Moreover, Co-IP assays showed that Paxillin and USP13 interacted with each other (Fig.  xref d, e)."

sparser
"Furthermore, Paxillin and Paxillin(Y118A) interacted with USP13, but Paxillin(Y31A) failed to associate with USP13 (Fig.  xref l), implicating that Paxillin Y31 phosphorylation is important for USP13-Paxillin interaction."

sparser
"Additionally, USP13(1–863) and USP13(1–300) interacted with Paxillin, but USP13(301–624), USP13(301–863), and USP13(625–863) could not interact with Paxillin (Fig.  xref k), indicating that 1aa–300aa of USP13 is involved in USP13-Paxillin interaction."

reach
"Deubiquitinase USP13 promotes the epithelial-mesenchymal transition and metastasis in gastric cancer by maintaining Snail protein."

reach
"In vivo, the expression of WT-USP13, particularly USP13-Y708E construct in HCCC9810 cells, significantly enhanced the metastasis and growth of CCA cells, while the USP13-Y708F construct was resistant to carcinogenesis after implanting in nude mice (XREF_FIG)."

reach
"USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model."

reach
"USP13 is highly amplified in human ovarian cancer, and elevated expression of USP13 promotes tumorigenesis and metastasis of ovarian cancer."

reach
"Notably, USP13 greatly enhanced peritoneal metastasis of ovarian tumors with frequent development of hemorrhagic ascites."
USP13 affects NPLOC4
1 | 1 3
| 2

sparser
"It was shown that USP13 associates with p97/VCP, Ufd1, Npl4, and UBXD8, and that depletion of USP13 leads to the accumulation of ERAD substrates [ xref ]."

sparser
"USP13 interacts with p97/VCP, Ufd1, Npl4, and UBXD8 [ xref ]."
1 | 1

No evidence text available

reach
"USP13 interacts with p97 and VCP, Ufd1, Npl4, and UBXD8 [XREF_BIBR]."
| 1

sparser
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
USP13 affects MITF
| 3
USP13 activates MITF.
| 2
USP13 activates MITF. 2 / 3
| 2

reach
"On the other hand, overexpression of the deubiquitinase USP13, which has been identified as a key regulator of MITF turnover, can enhance the basal half-life of the MITF protein to up to 4h (Zhao etal., 2011)."

reach
"More recently, it was shown that MITF is targeted by the de-ubiquitinase USP13, a theoretically drug-able protease whose suppression results in strong downregulation of MITF protein levels."
USP13 increases the amount of MITF.
| 1
USP13 increases the amount of MITF. 1 / 2
| 1

reach
"By modulate the expression of MITF downstream genes, USP13 appeared to be essential for melanoma growth both in vivo and in vitro, indicating the possibility of targeting USP13 for melanoma therapy."
USP13 affects Interferon
| 1 4
| 1 4

reach
"USP13 has been reported to deubiquitinate and stabilize STAT1 and promote interferon (IFN)-induced signalling XREF_BIBR."

reach
"USP13 is considered to promote IFN signaling and play an antiviral role by stabilizing STAT1 [55]."

reach
"Thus, USP13 positively regulates type I and type II IFN signaling by deubiquitinating and stabilizing STAT1 protein."

reach
"However, USP13, USP19, and USP22 inhibit virus-induced IFN production by removing K27-linked polyubiquitin chains from STING (40, 81) or TRIF (90)."

eidos
"It has also been reported that USP13 promotes interferon signaling by stabilizing STAT1 [ 25 ] ."
USP13 affects GSC
| 5
USP13 activates GSC.
| 4
USP13 activates GSC. 4 / 4
| 4

reach
"In vivo bioluminescent analysis confirmed that induced disruption of USP13 by doxycycline treatment significantly inhibited GSC tumor growth in mouse intracranial xenografts (XREF_FIG)."

reach
"Disrupting USP13 abrogates GSC tumor growth."

reach
"In addition, IHC staining of Ki-67 and cleaved caspase 3 confirmed that USP13 disruption reduced c-Myc protein in the GSC derived xenografts and led to a significant decrease in cell proliferation and an increase in cell apoptosis within the tumor (not depicted)."

reach
"In addition, USP13 disruption significantly reduced GSC proliferation (XREF_FIG) but showed little effects on NSTCs and NPCs (not depicted)."
USP13 inhibits GSC.
| 1
USP13 inhibits GSC. 1 / 1
| 1

reach
"Importantly, an orthotopic tumor formation assay demonstrated that ectopic expression of the ubiquitin-insensitive c-Myc mutant (T58A-c-Myc) restored GSC tumor growth which was attenuated by USP13 down-regulation or FBXL14 up-regulation (XREF_FIG)."
USP13 affects FN1
| 5
USP13 increases the amount of FN1. 4 / 4
| 4

reach
"We have shown that USP13 knockout reduced fibronectin expression in lungs."

reach
"It has been shown that USP13 knockout reduced fibronectin expression in lungs."

reach
"Overexpression of USP13 increased FN and Smad4 protein levels in lung fibroblasts, while downregulation of USP13 reduced Smad4 protein levels, without altering Smad4 mRNA expression, suggesting that USP13 regulates Smad4 protein stability."

reach
"Our previous study showed that USP13 reduction decreased fibronectin expression in lung fibroblast cells [18]."
Modified USP13 increases the amount of FN1. 1 / 1
| 1

reach
"Overexpression of USP13 increased FN and Smad4 protein levels in lung fibroblasts, while downregulation ofUSP13 reduced Smad4 protein levels, without altering Smad4 mRNA expression, suggesting that USP13 regulates Smad4 protein stability."
USP13 affects FBXL14
| 1 4
USP13 binds FBXL14 and MYC. 4 / 4
| 4

sparser
"These results demonstrate that c-Myc interacts with USP13 and FBXL14 in glioma cells, indicating that deubiquitinase USP13 and the ubiquitin E3 ligase FBXL14 are potential regulators of c-Myc protein stability in GBM."

sparser
"The interaction between c-Myc and USP13 or FBXL14 was validated in HEK293 cells coexpressing c-Myc and Flag-tagged USP13 or FBXL14."

sparser
"To further confirm the interaction between endogenous c-Myc and USP13 or FBXL14 in GSCs, c-Myc was immunoprecipitated from cell lysate of partially differentiated GSCs that were induced by serum acutely (2 d)."

sparser
"USP13 and FBXL14 interact with c-Myc in glioma cells."
| 1

reach
"The interaction between c-Myc and USP13 or FBXL14 was validated in HEK293 cells coexpressing c-Myc and Flag tagged USP13 or FBXL14."
USP13 affects ERAD
| 1 4
USP13 activates ERAD.
| 1 2
USP13 activates ERAD. 3 / 3
| 1 2

reach
"By contrast, USP13 depletion did not affect deglycosylation of TCRalpha (lane 2 vs 1), suggesting that USP13 promotes ERAD downstream of p97."

eidos
"On the other hand , USP13 antagonizes AMFR by deubiquitinating UBL4A to maintain the integrity of BAG6 complex and therefore promotes ERAD ( Liu et al ., 2014 ) ."

reach
"Despite having opposing activities, USP13 can cooperate with gp78 to promote ERAD."
USP13 inhibits ERAD.
| 2
USP13 inhibits ERAD. 2 / 2
| 2

reach
"The fact that similar ERAD phenotypes were detected under both USP13 overexpression and depletion conditions further confirmed that USP13 overexpression inhibits ERAD by a dominant negative manner."

reach
"The requirement of gp78 interaction for ERAD inhibition by ectopic USP13 indicates that the interaction of USP13 with gp78 is functionally relevant to ERAD."
UBL4A affects USP13
4 | 1
4 | 1

sparser
"Additionally, as catalytic-inactive DUBs often trap their substrates, can a catalytic-inactive USP13 bind preferentially to Ubl4A?"

No evidence text available

No evidence text available

No evidence text available

No evidence text available
SIGIRR affects USP13
| 2 3
| 2 3

sparser
"USP13 is associated with Sigirr."

sparser
"Co-immunoprecipitation (Co-IP) also revealed USP13's association with Sigirr ( xref b, c)."

reach
"To determine molecular mechanisms by which GSK3β promotes Sigirr degradation, we examined the effect of GSK3β-mediated phosphorylation on the association between Sigirr and USP13."

sparser
"Overexpression of GSK3β reduced the association between Sigirr and USP13 ( xref ), suggesting that GSK3β-mediated phosphorylation prevents Sigirr degradation from Sigirr interaction with its stabilizer, USP13."

reach
"Overexpression of GSK3β reduced the association between Sigirr and USP13 (Fig. 7B), suggesting that GSK3β-mediated phosphorylation prevents Sigirr degradation from Sigirr interaction with its stabilizer, USP13."
PXN affects USP13
| 5
| 5

sparser
"USP13 interacts with Paxillin and NLRP3, phosphorylation of Paxillin at Y31 is important for Paxillin-USP13 interaction, and USP13 is essential for Paxillin-mediated NLRP3 deubiquitination upon ATP treatment."

sparser
"Moreover, the domains of USP13 required for USP13-NLRP3 interaction and USP13-Paxillin association were assessed by constructing and analyzing plasmids encoding for WT USP13(1–863) and four truncated proteins (Fig.  xref i)."

sparser
"Moreover, Co-IP assays showed that Paxillin and USP13 interacted with each other (Fig.  xref d, e)."

sparser
"Furthermore, Paxillin and Paxillin(Y118A) interacted with USP13, but Paxillin(Y31A) failed to associate with USP13 (Fig.  xref l), implicating that Paxillin Y31 phosphorylation is important for USP13-Paxillin interaction."

sparser
"Additionally, USP13(1–863) and USP13(1–300) interacted with Paxillin, but USP13(301–624), USP13(301–863), and USP13(625–863) could not interact with Paxillin (Fig.  xref k), indicating that 1aa–300aa of USP13 is involved in USP13-Paxillin interaction."
PIK3C3 affects USP13
| 5
PIK3C3 inhibits USP13.
| 2
PIK3C3 inhibits USP13. 2 / 2
| 2

reach
"Using an imaging-based screen, Liu et al. (2011) recently identified a highly potent small molecule inhibitor of autophagy they named spautin-1 (specific and potent autophagy inhibitor 1), which promotes degradation of the Vps34 complexes via inhibiting ubiquitin-specific processing protease 10 (USP10) and USP13, two ubiquitin-specific peptidases that target the deubiquitination of Beclin1.2."

reach
"It is interesting to note that the knock-down of VPS34 and BECLIN 1 also causes the degradation of USP10 and USP13, which indicates the existence of a regulatory feedback loop [XREF_BIBR]."
PIK3C3 activates USP13.
| 2
PIK3C3 activates USP13. 2 / 2
| 2

reach
"Another interesting observation made in the Spautin-1 study is that knock-down of VPS34 and Beclin 1 causes instability of USP10 and USP13, which indicates the existence of a regulatory feedback loop [XREF_BIBR]."

reach
"The close functional interconnection between the central PI3K-III subunits and the DUBs is demonstrated by the interesting observation that knock-down of VPS34 and Beclin 1 causes instability of USP10 and USP13, which strongly indicates the existence of a regulatory feedback loop [XREF_BIBR]."
PIK3C3 increases the amount of USP13.
| 1
PIK3C3 increases the amount of USP13. 1 / 1
| 1

reach
"Unexpectedly, we found that the knockdown of Beclin1 or Vps34 could also reduce the endogenous levels of USP10 and USP13 (XREF_FIG)."
OGDH affects USP13
2 | 3
2 | 1

No evidence text available

No evidence text available

sparser
"The N terminus including a ubiquitin-specific protease-type zinc-finger domain (residues 1–336) was essential for USP13 to interact with OGDH, whereas the ubiquitin carboxyl-terminal hydrolase domain (residues 336–863) of USP13 is required for its binding with ACLY ( xref )."
ACLY binds USP13 and OGDH. 2 / 2
| 2

sparser
"Reciprocal analyses in which immunoprecipitated ACLY or OGDH were probed for USP13 confirmed the USP13ACLY and USP13OGDH interactions ( xref )."

sparser
"Western blot analyses of HEK293T cell lysates immunoprecipitated with USP13 antibody showed the interaction of USP13 with ACLY and OGDH ( xref )."
NPLOC4 affects USP13
1 | 1 3
| 2

sparser
"It was shown that USP13 associates with p97/VCP, Ufd1, Npl4, and UBXD8, and that depletion of USP13 leads to the accumulation of ERAD substrates [ xref ]."

sparser
"USP13 interacts with p97/VCP, Ufd1, Npl4, and UBXD8 [ xref ]."
1 | 1

No evidence text available

reach
"USP13 interacts with p97 and VCP, Ufd1, Npl4, and UBXD8 [XREF_BIBR]."
| 1

sparser
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
FBXL14 affects USP13
| 1 4
USP13 binds FBXL14 and MYC. 4 / 4
| 4

sparser
"These results demonstrate that c-Myc interacts with USP13 and FBXL14 in glioma cells, indicating that deubiquitinase USP13 and the ubiquitin E3 ligase FBXL14 are potential regulators of c-Myc protein stability in GBM."

sparser
"The interaction between c-Myc and USP13 or FBXL14 was validated in HEK293 cells coexpressing c-Myc and Flag-tagged USP13 or FBXL14."

sparser
"To further confirm the interaction between endogenous c-Myc and USP13 or FBXL14 in GSCs, c-Myc was immunoprecipitated from cell lysate of partially differentiated GSCs that were induced by serum acutely (2 d)."

sparser
"USP13 and FBXL14 interact with c-Myc in glioma cells."
| 1

reach
"The interaction between c-Myc and USP13 or FBXL14 was validated in HEK293 cells coexpressing c-Myc and Flag tagged USP13 or FBXL14."
CDH1 affects USP13
| 2 3
| 2 3

sparser
"We performed immunoprecipitation assays followed by western-blotting to assess the interaction between USP13 and CDH1."

sparser
"Shortly after this finding, the interaction of USP13 with CDH1 was also confirmed by the same team ( xref )."

reach
"We performed immunoprecipitation assays followed by western-blotting to assess the interaction between USP13 and CDH1."

reach
"We found that USP13 binds to the APC/C adaptor CDH1."

sparser
"We found that USP13 binds to the APC/C adaptor CDH1."
MiR-130b/301b affects USP13
| 4
Modified miR-130b/301b decreases the amount of USP13. 4 / 4
| 4

reach
"Results showed that overexpression of miR-130b/301b significantly decreased USP13 expression in transcription and translational levels, and knockdown of miR-130b/301b revealed an opposite effect on USP13 expression."

reach
"Overexpression of miR-130b/301b or USP13 knockdown by shRNA delivery decreases USP13 expression, and further reduces PTEN protein expression and leads to enhancement of cell proliferation, invasion and migration."

reach
"Results showed that overexpression of miR-130b/301b dramatically decreased protein expression of USP13 and PTEN, while restoration of USP13 completely reversed the effect."

reach
"We reported a potential regulatory loop that the NF-kB-induced miR-130b/301b overexpression decreased USP13 expression and subsequently resulted in the downregulation of PTEN protein and promoted tumorigenesis of bladder cancer."
USP13 affects UFD1
2 | 1
2 |

No evidence text available

No evidence text available
| 1

sparser
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
USP13 affects UBC
4 |
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
USP13 affects TOPBP1
| 1 3
| 1 3

sparser
"Mechanistically, USP13 binds to TopBP1 and stabilizes TopBP1 by deubiquitination."

sparser
"Our study shows that USP13 interacts with TopBP1."

reach
"Mechanistically, USP13 binds to TopBP1 and stabilizes TopBP1 by deubiquitination."

sparser
"To test whether USP13 is a deubiquitinase for TopBP1, we first examined whether USP13 interacts with TopBP1."
USP13 affects PIK3C3
| 4
USP13 activates PIK3C3.
| 2
USP13 activates PIK3C3. 2 / 2
| 2

reach
"Interestingly, Spautin-1-mediated specific and potent inhibition of deubiquitinating activity of USP10 and USP13 was shown to promote proteasomal degradation of PIK3C3 and thereby inhibit autophagy [179]."

reach
"We propose that USP10 and USP13 mediate the deubiqutination of Vps34 complexes to regulate the levels of class III PI3 kinase."
USP13 ubiquitinates PIK3C3.
| 1
USP13 leads to the ubiquitination of PIK3C3. 1 / 1
| 1

reach
"Our results suggest that inhibition of USP10 and USP13 by spautin-1 promotes the ubiquitination and degradation of Vps34 complexes which in turn leads to a reduction in the levels of PtdIns3P and consequent inhibition of autophagy."
USP13 inhibits PIK3C3.
| 1
USP13 inhibits PIK3C3. 1 / 1
| 1

reach
"USP10 and USP13, two ubiquitin‐specific peptidases, can target the Beclin1 subunit of Vps34‐Beclin1 and promote the degradation of the Vps34 complex."
USP13 affects NLRP3
| 1 3
USP13 binds NLRP3.
| 3
| 3

sparser
"Co-IP assays showed that NLRP3 and USP13 interacted with each other (Fig.  xref f, g)."

sparser
"Moreover, the domains of USP13 required for USP13-NLRP3 interaction and USP13-Paxillin association were assessed by constructing and analyzing plasmids encoding for WT USP13(1–863) and four truncated proteins (Fig.  xref i)."

sparser
"Like WT USP13(1–863), USP13(301–863) and USP13(625–863) interacted with NLRP3, but USP13(1–300) or USP13(301–624) failed to interact with NLRP3 (Fig.  xref j), indicating that 625aa–863aa of USP13 are involved in USP13-NLRP3 interaction."
USP13 activates NLRP3.
| 1
USP13 activates NLRP3. 1 / 1
| 1

reach
"Overexpression of USP13 in PC12 cells almost reversed the Spautin‐1‐mediated decrease in NLRP3, USP13, Beclin1 and GSDMD‐N expression levels (Figure 3D,E)."
USP13 affects MDC1
| 1 3
| 1 3

sparser
"To further demonstrate that the FHA domain of MDC1 is critical for USP13-MDC1 interaction, we purified GST-MDC1-FHA fragment from bacteria and performed pull-down assay."

sparser
"Moreover, we examined the interaction between USP13 and different deletion mutants of MDC1 and found that deletion of FHA domain of MDC1 abolishes the USP13-MDC1 interaction ( xref )."

sparser
"In addition, USP13 interacted with MDC1 and the interaction between USP13 and MDC1 was dramatically increased following cisplatin treatment ( xref )."

reach
"In addition, USP13 interacted with MDC1 and the interaction between USP13 and MDC1 was dramatically increased following cisplatin treatment (XREF_FIG)."
USP13 affects FAF2
1 | 3
| 2

sparser
"It was shown that USP13 associates with p97/VCP, Ufd1, Npl4, and UBXD8, and that depletion of USP13 leads to the accumulation of ERAD substrates [ xref ]."

sparser
"USP13 interacts with p97/VCP, Ufd1, Npl4, and UBXD8 [ xref ]."
1 |

No evidence text available
| 1

sparser
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
| 4

reach
"USP13 is highly amplified in human ovarian cancer, and elevated expression of USP13 promotes tumorigenesis and metastasis of ovarian cancer."

reach
"In vitro and in vivo depletion of USP13 inhibited SCLC cancer stem cells (CSCs) properties and tumorigenesis, and this inhibitory effect was rescued by reconstituted expression of wide type (WT) USP13 but not the enzyme-inactive USP13 mutant."

reach
"More importantly, the small molecule inhibitor of FASN, TVB-2640, significantly inhibits lipogenic phenotype and attenuates self-renewal ability, chemotherapy resistance and USP13-mediated tumorigenesis in SCLC."

reach
"Overexpression of USP13 with deletion of Trp53 and Pten in murine ovarian surface epithelium accelerated ovarian tumorigenesis and led to decreased survival in mice."
USP13 affects COL9A2
| 4
| 4

sparser
"In USP13MED, miR‐367 silencing significantly inhibited proliferation of cells displaying either basal or enhanced OCT4A expression (Fig. xref B,C)."

sparser
"The therapeutic potential of miR‐367 was further confirmed in vivo , in an orthotopic xenograft model of aggressive embryonal CNS tumors initiated by OCT4A‐overexpressing cells (Daoy, USP13MED, or USP7‐ATRT) in Balb/C nude mice."

sparser
"The overall survival rate of animals harboring Daoy, USP13MED, or USP7‐ATRT‐initiated tumors was also significantly improved by treatment with miR‐367 inhibitor (Fig. xref D)."

sparser
"Finally, miR‐367 silencing also significantly inhibited 3D cell invasion in tumor spheroids of both USP13MED and USP7‐ATRT cells (Fig. xref ), suggesting that miR‐367 silencing inhibits two key features of tumor aggressiveness, namely, stem‐like properties and invasive behavior."
UFD1 affects USP13
2 | 1
2 |

No evidence text available

No evidence text available
| 1

sparser
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
UBC affects USP13
4 |
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
TOPBP1 affects USP13
| 1 3
| 1 3

sparser
"Mechanistically, USP13 binds to TopBP1 and stabilizes TopBP1 by deubiquitination."

sparser
"Our study shows that USP13 interacts with TopBP1."

reach
"Mechanistically, USP13 binds to TopBP1 and stabilizes TopBP1 by deubiquitination."

sparser
"To test whether USP13 is a deubiquitinase for TopBP1, we first examined whether USP13 interacts with TopBP1."
TLR4 affects USP13
| 2 2
TLR4 binds USP13.
| 1 2
| 1 2

reach
"USP13 interacted with TLR4 and inhibited the ubiquitin mediated degradation of TLR4."

sparser
"The co-IP assay revealed that USP13 directly interacted with TLR4 in Hep3B cells ( xref )."

sparser
"USP13 interacted with TLR4 and inhibited the ubiquitin-mediated degradation of TLR4."
TLR4 activates USP13.
| 1
TLR4 activates USP13. 1 / 1
| 1

reach
"Significantly, TLR4 re-expression remarkably reversed the effects of USP13 knockdown on HCC cells."
STAT1 affects USP13
2 | 2
2 | 1

No evidence text available

No evidence text available

sparser
"Usp13 mainly interacted with the nonactivated form of Stat1 ( xref )."
| 1

sparser
"For example, the three deubiquitinating enzymes BRCC36, USP13, and USP39 interact with STAT1 and decrease the K63-, K48- and K6-linked polyubiquitin chains of STAT1 respectively (110, 115, 118)."
SKP2 affects USP13
1 | 1 2
1 | 1 1

reach
"We found that SKP2 interacted with USP10, USP13, UCHL5, USP14, and USP7 in KBM5-T315I cells."

No evidence text available

sparser
"UFD1 was reported to function as a scaffold for the USP13-Skp2 (the F-box adaptor of the E3 ubiquitin ligase SCF Skp2 ) interaction, maintaining Skp2 stability."
SKP2 binds USP10 and USP13. 1 / 1
| 1

sparser
"The present study uncovers that USP10 is another critical DUB that deubiquitinates SKP2 and suppresses the ubiquitin-dependent proteasomal degradation of SKP2, which is compellingly supported by multiple lines of evidence: (1) our Co-IP experiments revealed compelling interaction of endogenous SKP2 with not only USP13 but also USP10 in KBM5-T315I and KBM5 cells (Fig.  xref ); (2) the interaction of overexpressed SKP2 with overexpressed USP13 or USP10 was also confirmed in HEK293T cells but the USP10 missing the N-terminal 205 amino acid residues failed to bind to SKP2 (Fig.  xref ); (3) overexpression of either USP13 or USP10 led to a higher SKP2 protein level and decreased p27 but overexpression of a DUB activity-disabled USP10 failed to do so (Fig.  xref ); (4) shRNA-mediated knockdown or pharmacological inhibition of USP10 with Spautin1 remarkable decreased SKP2 and phosphorylated Bcr-Abl (but not total Bcr-Abl) but increased p27 in multiple CML cell lines (Fig.  xref , Fig.  xref ); (5) CHX chase assays showed that shortened the halflife of SKP2 protein (Fig.  xref ); and (6) both pharmacological and genetic inhibition of USP10 could markedly increase K48-linked ubiquitinated SKP2 (Fig.  xref )."
SIAH2 affects USP13
2 | 1 1
SIAH2 binds USP13.
2 | 1
2 | 1

sparser
"Overall, this study identifies a new layer of Siah2 regulation mediated by USP13 binding to ubiquitinated Siah2 protein with a concomitant inhibitory effect on its activity under normoxia."

No evidence text available

No evidence text available
SIAH2 activates USP13.
| 1
SIAH2 activates USP13. 1 / 1
| 1

reach
"Thus, USP13 limits Siah2 autodegradation and its ubiquitin ligase activity against its target substrates."
MYC affects FBXL14
| 4
USP13 binds FBXL14 and MYC. 4 / 4
| 4

sparser
"These results demonstrate that c-Myc interacts with USP13 and FBXL14 in glioma cells, indicating that deubiquitinase USP13 and the ubiquitin E3 ligase FBXL14 are potential regulators of c-Myc protein stability in GBM."

sparser
"The interaction between c-Myc and USP13 or FBXL14 was validated in HEK293 cells coexpressing c-Myc and Flag-tagged USP13 or FBXL14."

sparser
"To further confirm the interaction between endogenous c-Myc and USP13 or FBXL14 in GSCs, c-Myc was immunoprecipitated from cell lysate of partially differentiated GSCs that were induced by serum acutely (2 d)."

sparser
"USP13 and FBXL14 interact with c-Myc in glioma cells."
MDC1 affects USP13
| 1 3
| 1 3

sparser
"To further demonstrate that the FHA domain of MDC1 is critical for USP13-MDC1 interaction, we purified GST-MDC1-FHA fragment from bacteria and performed pull-down assay."

sparser
"Moreover, we examined the interaction between USP13 and different deletion mutants of MDC1 and found that deletion of FHA domain of MDC1 abolishes the USP13-MDC1 interaction ( xref )."

sparser
"In addition, USP13 interacted with MDC1 and the interaction between USP13 and MDC1 was dramatically increased following cisplatin treatment ( xref )."

reach
"In addition, USP13 interacted with MDC1 and the interaction between USP13 and MDC1 was dramatically increased following cisplatin treatment (XREF_FIG)."
FBXL14 affects MYC, and USP13
| 4
USP13 binds FBXL14 and MYC. 4 / 4
| 4

sparser
"These results demonstrate that c-Myc interacts with USP13 and FBXL14 in glioma cells, indicating that deubiquitinase USP13 and the ubiquitin E3 ligase FBXL14 are potential regulators of c-Myc protein stability in GBM."

sparser
"The interaction between c-Myc and USP13 or FBXL14 was validated in HEK293 cells coexpressing c-Myc and Flag-tagged USP13 or FBXL14."

sparser
"To further confirm the interaction between endogenous c-Myc and USP13 or FBXL14 in GSCs, c-Myc was immunoprecipitated from cell lysate of partially differentiated GSCs that were induced by serum acutely (2 d)."

sparser
"USP13 and FBXL14 interact with c-Myc in glioma cells."
FAF2 affects USP13
1 | 3
| 2

sparser
"It was shown that USP13 associates with p97/VCP, Ufd1, Npl4, and UBXD8, and that depletion of USP13 leads to the accumulation of ERAD substrates [ xref ]."

sparser
"USP13 interacts with p97/VCP, Ufd1, Npl4, and UBXD8 [ xref ]."
1 |

No evidence text available
| 1

sparser
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
COL9A2 affects USP13
| 4
| 4

sparser
"In USP13MED, miR‐367 silencing significantly inhibited proliferation of cells displaying either basal or enhanced OCT4A expression (Fig. xref B,C)."

sparser
"The therapeutic potential of miR‐367 was further confirmed in vivo , in an orthotopic xenograft model of aggressive embryonal CNS tumors initiated by OCT4A‐overexpressing cells (Daoy, USP13MED, or USP7‐ATRT) in Balb/C nude mice."

sparser
"The overall survival rate of animals harboring Daoy, USP13MED, or USP7‐ATRT‐initiated tumors was also significantly improved by treatment with miR‐367 inhibitor (Fig. xref D)."

sparser
"Finally, miR‐367 silencing also significantly inhibited 3D cell invasion in tumor spheroids of both USP13MED and USP7‐ATRT cells (Fig. xref ), suggesting that miR‐367 silencing inhibits two key features of tumor aggressiveness, namely, stem‐like properties and invasive behavior."
Ubiquitin-proteasome affects USP13
| 3
Ubiquitin-proteasome inhibits USP13. 3 / 3
| 3

reach
"Bortezomib treatment increased accumulation of USP13 (Fig. 4E), indicating that USP13 degradation is mediated by the ubiquitin-proteasome system."

reach
"Here, we report that USP13 degradation is mediated by the ubiquitin-proteasome system."

reach
"Inhibition of proteasome, but not lysosome or immunoproteasome, attenuates LPS-induced USP13 degradation, suggesting USP13 degradation is mediated by the ubiquitin-proteasome system."
Ubiquitin-proteasome system affects USP13
| 3
Ubiquitin-proteasome system inhibits USP13. 3 / 3
| 3

eidos
"Inhibition of proteasome , but not lysosome or immunoproteasome , attenuates LPS-induced USP13 degradation , suggesting USP13 degradation is mediated by the ubiquitin-proteasome system ."

eidos
"Here , we report that USP13 degradation is mediated by the ubiquitin-proteasome system ."

eidos
"Bortezomib treatment increased accumulation of USP13 ( Fig. 4E ) , indicating that USP13 degradation is mediated by the ubiquitin-proteasome system ."
Ubiquitin affects UBA1
| 3
| 3

sparser
"The tandem UBA domains of USP13 interact with Ub."

sparser
"Moreover, the individual UBA domains (UBA1 and UBA2) of USP13 can also bind with Ub ( xref )."

sparser
"Notably, the tandem UBA domains of USP13 could bind to Ub and catalyze the hydrolysis of the polyUb chains (Lys-63/48/27) ( xref ; xref ; xref )."
USP13 affects miR-301b-3p
| 3
USP13 activates miR-301b-3p. 3 / 3
| 3

reach
"Furthermore, we discovered that USP13 was a common target of miR-130b-3p and miR-301b-3p, and the miR-130b/301b cluster, which could be transcriptionally upregulated by NF-kB."

reach
"Man et al. [XREF_BIBR] reported that USP13 is a direct target of miR-301b-3p, and it can be downregulated by miR-301b-3p overexpression, leading to decreased protein expression of PTEN, consequently promoting the occurrence of bladder cancer."

reach
"USP13 is a common target of mR-130b-3p and miR-301b-3p, and is down-regulated in human bladder cancer tumors."
USP13 affects cell cycle
| 1 2
USP13 activates cell cycle.
| 1 1
| 1 1

reach
"In addition, reconstitution of WT USP13, but not the T196A mutant, fully rescued the foci formation of the BRCA1-A complex, DNA repair and cell cycle checkpoint in USP13 deficient cells and reversed hypersensitivity to cisplatin or olaparib induced by USP13 deficiency (XREF_FIG and XREF_SUPPLEMENTARY)."

eidos
"This result indicates that USP13 deficiency or inhibition prevents cell cycle arrest in S phase in response to replication stress ."
USP13 inhibits cell cycle.
| 1
| 1

reach
"Cotargeting of EGFR and USP13 led to a robust increase in treatment efficacy both in vitro and in vivo, mainly due to a strong induction of apoptosis rather than enhancing the cell cycle arrest caused by afatinib alone."
USP13 affects afatinib
| 1 2
| 1 2

reach
"Overall, these data indicate that targeting of USP13 strongly potentiates the anticancer effects of afatinib in vivo."

reach
"Similar results were obtained in HCC827 cells, confirming that USP13 knockdown strongly enhances afatinib in treatment-naive lung cancer cell lines."

eidos
"Similar results were obtained in HCC827 cells ( Figure 2D ) , confirming that USP13 knockdown strongly enhances afatinib in treatment-naive lung cancer cell lines ."
USP13 affects UBA1
| 3
| 3

sparser
"The tandem UBA domains of USP13 interact with Ub."

sparser
"Moreover, the individual UBA domains (UBA1 and UBA2) of USP13 can also bind with Ub ( xref )."

sparser
"Notably, the tandem UBA domains of USP13 could bind to Ub and catalyze the hydrolysis of the polyUb chains (Lys-63/48/27) ( xref ; xref ; xref )."
USP13 affects TXNIP
| 3
USP13 increases the amount of TXNIP. 3 / 3
| 3

reach
"USP13 Deficiency Reduces TXNIP Levels in Lung Cells."

reach
"Knockdown of USP13 significantly reduced TXNIP levels in lungs or lung fibroblast cells."

reach
"Knockout or knockdown of USP13 in lungs or lung fibroblast cells significantly reduced TXNIP levels."
USP13 affects TRIM63
1 1 | 1
1 1 | 1

No evidence text available

reach
"MURF-1 also binds to ubiquitin conjugating enzyme-9 and isopeptidase T-3, enzymes involved in small ubiquitin related modifier mediated nuclear import, and with glucocorticoid modulatory element binding protein-1 (GMEB-1), a transcriptional regulator."

No evidence text available
USP13 affects TLR4
| 1 2
| 1 2

reach
"USP13 interacted with TLR4 and inhibited the ubiquitin mediated degradation of TLR4."

sparser
"The co-IP assay revealed that USP13 directly interacted with TLR4 in Hep3B cells ( xref )."

sparser
"USP13 interacted with TLR4 and inhibited the ubiquitin-mediated degradation of TLR4."
USP13 affects SMC3
2 | 1
USP13 binds SMC3.
2 |
2 |

No evidence text available

No evidence text available
USP13 decreases the amount of SMC3.
| 1
USP13 decreases the amount of ubiquitinated SMC3. 1 / 1
| 1

reach
"Ectopic overexpression of USP13 significantly decreased the levels of endogenous ubiquitinated SMC3 and RAD21, indicating that USP13 can function as a deubiquitinase for cohesin subunits."
USP13 affects Proteasome
| 3
USP13 activates Proteasome.
| 2
| 2

reach
"Notably, USP13 not only bound Bag6, but also interacted with MMS1 and alpha6, subunits of the proteasome (lane 5), further implicating USP13 in delivery pathways that targets substrates to the proteasome."

reach
"USP13 is upregulated in Alzheimer's disease (AD) and Parkinson's disease (PD), and USP13 knockdown via shRNA reduces neurotoxic proteins and increases proteasome activity in models of neurodegeneration."
USP13 inhibits Proteasome.
| 1
| 1

reach
"USP13 knockdown significantly increased the activity of the 20S proteasome and reduced the levels of hyper-phosphorylated tau (p-tau) in primary cortical neurons."
USP13 affects NFE2L2
| 3
USP13 inhibits NFE2L2.
| 2
USP13 inhibits NFE2L2. 2 / 2
| 2

reach
"USP13 reduces septic mediated cardiomyocyte oxidative stress and inflammation by inducing Nrf2."

reach
"Further studies showed that USP13 reduced LPS-induced oxidative stress and inflammation by inducing Nrf2."
USP13 increases the amount of NFE2L2.
| 1
USP13 increases the amount of NFE2L2. 1 / 1
| 1

reach
"In conclusion, this study demonstrated that USP13 was downregulated in septic myocardial injury tissues, and USP13 overexpression increased Nrf2 levels and reduced apoptosis."
USP13 affects HMOX1
| 3
USP13 decreases the amount of HMOX1. 3 / 3
| 3

reach
"Overexpression of USP13 reduced HMOX1 protein levels in lung fibroblast cells, suggesting that modulation of USP13 levels may affect oxidative stress."

reach
"Knockdown of USP13 by siRNA increased HMOX1 levels in lung fibroblast cells (Mrc5) and epithelial cells (A549 and BEAS-2B) (Fig. 2B, D, E), while HMOX1 levels were decreased in V5-tagged USP13-overexpressed Mrc5 cells (Fig. 2C), indicating that USP13 regulates HMOX1 levels in lung cells."

reach
"Knockdown of USP13 increased HMOX1 basal levels but did not rescue the HMOX1 reduction by TGF-β1 (Fig. 2B)."
USP13 affects GST
| 3
GST binds USP13 and VCP. 1 / 1
| 1

sparser
"We expressed these proteins in cells individually and examined the binding of endogenous p97 to GST-USP13."
GST binds USP13, USP5, and VCP. 1 / 1
| 1

sparser
"By contrast, neither GST-USP13 nor GST-USP5 bound p97 ( xref )."
GST binds USP13 and BAG6. 1 / 1
| 1

sparser
"Indeed, GST pull-down experiments using purified proteins showed that GST-USP13 bound Bag6, and the N-terminal ubiquitin-like (UBL) domain in Bag6 was both necessary and sufficient for interaction with USP13 ( xref )."
| 2 1
| 2 1

eidos
"The knockdown of USP13 also led to a substantial cell viability reduction upon osimertinib treatment ( Figure 2E ) ."

eidos
"( A ) USP13 knockdown decreases cell viability with or without MG132 treatment ."

reach
"However, in the context of hypoxic or oxidative stresses, USP13 loss caused a substantial decrease of cell viability and a significant increase of cell apoptosis in both TOV-21G and SW-1573 tumor models."
USP13 affects Beclin1 subunit
| 3
USP13 activates Beclin1 subunit. 3 / 3
| 3

reach
"Spautin-1 promotes the degradation of Vps34 PI3 kinase complexes by inhibiting two ubiquitin specific peptidases, USP10 and USP13, that target the Beclin1 subunit of Vps34 complexes."

reach
"We treated TBEV infected neuroblastoma cells with rapamycin (inducer of autophagy, as inhibition of mTOR mimics cellular starvation by blocking signals required for cell growth and proliferation), and spautin-1 (highly specific and potent autophagy inhibitor in mammalian cells, which promotes the degradation of Vps34 PI3 kinase complexes by inhibiting two ubiquitin specific peptidases, USP10 and USP13 that target the Beclin1 subunit of Vps34 complexes) and investigated the effect of the treatment of TBEV growth."

reach
"Our second treatment was with Spautin-1 which promotes the degradation of Vps34 PI3 kinase complexes by inhibiting two ubiquitin specific peptidases, USP10 and USP13, which target the Beclin1 subunit of Vps34 complexes."
UBA1 affects Ubiquitin
| 3
| 3

sparser
"The tandem UBA domains of USP13 interact with Ub."

sparser
"Moreover, the individual UBA domains (UBA1 and UBA2) of USP13 can also bind with Ub ( xref )."

sparser
"Notably, the tandem UBA domains of USP13 could bind to Ub and catalyze the hydrolysis of the polyUb chains (Lys-63/48/27) ( xref ; xref ; xref )."
TRIM63 affects USP13
1 1 | 1
1 1 | 1

No evidence text available

reach
"MURF-1 also binds to ubiquitin conjugating enzyme-9 and isopeptidase T-3, enzymes involved in small ubiquitin related modifier mediated nuclear import, and with glucocorticoid modulatory element binding protein-1 (GMEB-1), a transcriptional regulator."

No evidence text available
Spautin‐1 affects USP13
| 3
Spautin‐1 inhibits USP13. 3 / 3
| 3

reach
"Spautin‐1 is an inhibitor of USP10 and USP13."

reach
"26 The role of autophagy in cerebral ischaemia‐reperfusion needs to be further investigated.Spautin‐1 was further used to investigate the mechanism of autophagy activation on cerebral ischaemia‐reperfusion injury, and Spautin‐1 is as an inhibitor of USP13."

reach
"Overexpression of USP13 in PC12 cells almost reversed the Spautin‐1‐mediated decrease in NLRP3, USP13, Beclin1 and GSDMD‐N expression levels (Figure 3D,E)."
NPLOC4 affects FAF2
| 3
| 2

sparser
"It was shown that USP13 associates with p97/VCP, Ufd1, Npl4, and UBXD8, and that depletion of USP13 leads to the accumulation of ERAD substrates [ xref ]."

sparser
"USP13 interacts with p97/VCP, Ufd1, Npl4, and UBXD8 [ xref ]."
| 1

sparser
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
NLRP3 affects USP13
| 3
| 3

sparser
"Co-IP assays showed that NLRP3 and USP13 interacted with each other (Fig.  xref f, g)."

sparser
"Moreover, the domains of USP13 required for USP13-NLRP3 interaction and USP13-Paxillin association were assessed by constructing and analyzing plasmids encoding for WT USP13(1–863) and four truncated proteins (Fig.  xref i)."

sparser
"Like WT USP13(1–863), USP13(301–863) and USP13(625–863) interacted with NLRP3, but USP13(1–300) or USP13(301–624) failed to interact with NLRP3 (Fig.  xref j), indicating that 625aa–863aa of USP13 are involved in USP13-NLRP3 interaction."
GST affects USP13
| 3
GST binds USP13 and VCP. 1 / 1
| 1

sparser
"We expressed these proteins in cells individually and examined the binding of endogenous p97 to GST-USP13."
GST binds USP13, USP5, and VCP. 1 / 1
| 1

sparser
"By contrast, neither GST-USP13 nor GST-USP5 bound p97 ( xref )."
GST binds USP13 and BAG6. 1 / 1
| 1

sparser
"Indeed, GST pull-down experiments using purified proteins showed that GST-USP13 bound Bag6, and the N-terminal ubiquitin-like (UBL) domain in Bag6 was both necessary and sufficient for interaction with USP13 ( xref )."
FAF2 affects NPLOC4
| 3
| 2

sparser
"It was shown that USP13 associates with p97/VCP, Ufd1, Npl4, and UBXD8, and that depletion of USP13 leads to the accumulation of ERAD substrates [ xref ]."

sparser
"USP13 interacts with p97/VCP, Ufd1, Npl4, and UBXD8 [ xref ]."
| 1

sparser
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
2 |
Valproic acid decreases the amount of USP13. 2 / 2
2 |

No evidence text available

No evidence text available
2 |
Polycyclic arene increases the amount of USP13.
1 |
Polycyclic arene increases the amount of USP13. 1 / 1
1 |

No evidence text available
Polycyclic arene decreases the amount of USP13.
1 |
Polycyclic arene decreases the amount of USP13. 1 / 1
1 |

No evidence text available
Phenylmercury acetate decreases the amount of USP13. 2 / 2
2 |

No evidence text available

No evidence text available
Imidazolide affects USP13
| 1 1
| 1 1

reach
"USP13 preferentially is induced in GIST cells by IM and interacts with ATG5, which leads to stabilization of ATG5 through deubiquitination."

eidos
"USP13 preferentially is induced in GIST cells by IM and interacts with ATG5 , which leads to stabilization of ATG5 through deubiquitination ."
Doxycycline affects USP13
| 2
| 2

reach
"In vivo bioluminescent analysis confirmed that induced disruption of USP13 by doxycycline treatment significantly inhibited GSC tumor growth in mouse intracranial xenografts (XREF_FIG)."

reach
"To further confirm the clinical relevance of targeting USP13 in established GBM tumors, we applied the Tet-on inducible knockdown system to examine whether inducible disruption of USP13 by doxycycline affects the growth of established xenograft tumors and animal survival."
2 |
Benzo[a]pyrene decreases the amount of USP13. 2 / 2
2 |

No evidence text available

No evidence text available
ZEB1 affects USP13
2 |
2 |

No evidence text available

No evidence text available
VCP affects GST
| 2
GST binds USP13 and VCP. 1 / 1
| 1

sparser
"We expressed these proteins in cells individually and examined the binding of endogenous p97 to GST-USP13."
GST binds USP13, USP5, and VCP. 1 / 1
| 1

sparser
"By contrast, neither GST-USP13 nor GST-USP5 bound p97 ( xref )."
VCL affects USP13
| 2
| 2

sparser
"However, sufficient follow-up validation studies are required in order to determine whether vinculin protein directly interacts with USP13."

sparser
"Among the identified proteins, the altered expression level of vinculin was associated with the DUB activity of USP13, which was confirmed by western blot analysis and reverse transcription-quantitative polymerase chain reaction (RT-qPCR)."
Ufd1 affects USP13
| 2
| 2

sparser
"It was shown that USP13 associates with p97/VCP, Ufd1, Npl4, and UBXD8, and that depletion of USP13 leads to the accumulation of ERAD substrates [ xref ]."

sparser
"USP13 interacts with p97/VCP, Ufd1, Npl4, and UBXD8 [ xref ]."
USP5 affects USP13
1 | 1
GST binds USP13, USP5, and VCP. 1 / 1
| 1

sparser
"By contrast, neither GST-USP13 nor GST-USP5 bound p97 ( xref )."
1 |

No evidence text available
USP13 affects tumor growth
| 2
USP13 activates tumor growth. 2 / 2
| 2

eidos
"Moreover , the DUB USP7 , USP13 , USP22 , USP28 , USP36 and USP37 stabilize c-Myc , thereby stimulating tumor growth [ 155 , 157 , 171-174 ] ."
| PMC

eidos
"Previous studies showed that two deubiquitinases , USP9X and USP13 , stabilize MCL-1 protein levels and promote tumor growth 5 , 7 ."
USP13 affects stabilization TLR4
| 2
USP13 activates stabilization TLR4. 2 / 2
| 2

eidos
"USP13 knockdown reduced the stabilization of TLR4 ."

eidos
"Here , we demonstrated that USP13 enhanced the stabilization of TLR4 via direct binding and deubiquitylation of TLR4 ."
USP13 affects stabilisation Mcl-1
| 2
USP13 activates stabilisation Mcl-1. 2 / 2
| 2

eidos
"These results suggest that the effect of USP13 on cell proliferation in cervical cancer cells is at least partially dependent on the stabilisation of Mcl-1 expression ."

eidos
"Taken together , these data suggest that USP13 mediated stabilisation of Mcl-1 may be a potential mechanism for the resistance of cervical cancer cells to BH3 mimetics ."
USP13 affects recruitment
| 1 1
USP13 inhibits recruitment. 2 / 2
| 1 1

sparser
"These data together suggest that USP13 inhibits the recruitment of TBK1 to STING by deubiquitinating STING."

eidos
"Previously , it has been demonstrated that USP13 removes K27-linked polyubiquitin chains from MITA and impairs its recruitment of TBK1 , contributing to inhibition of innate immune response to DNA viruses [ 36 ] ."
USP13 affects recruitment TBK1
| 2
USP13 inhibits recruitment TBK1. 2 / 2
| 2

eidos
"USP13 uncouples K27-linked polyubiquitin chains from STING and prevents the recruitment of TBK1 to inhibit STING pathway ( 84 ) ."

eidos
"USP13 has been shown to remove K27-linked ubiquitin chains from unidentified lysine residues in STING , thereby preventing the recruitment of TBK1 [ 171 ] ."
| 2
| 2

reach
"Different forms of stress modulate these activities : hypoxia reduces USP13 expression enabling increased Siah2 activity, while cellular stress induces p38 activity and alters Siah localization, affec[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Different forms of stress modulate these activities : hypoxia reduces USP13 expression enabling increased Siah2 activity, while cellular stress induces p38 activity and alters Siah localization, affecting its recognition and targeting of substrates 174."
USP13 affects half-life MITF-M
| 2
USP13 activates half-life MITF-M. 2 / 2
| 2

eidos
"In addition , USP13 upregulation mitigated the MITF-M degradation and significantly increased the half-life of MITF-M ."

eidos
"As per the outcomes , USP13 upregulation mitigated the MITF-M degradation and significantly increased the half-life of MITF-M ( Figure 5C ) ."
USP13 affects glioma stem cells
| 2
USP13 activates glioma stem cells. 2 / 2
| 2

eidos
"Moreover , USP13 depletion represses the proliferation of glioma stem cells ( GSCs ) and restrains tumor growth in mice ( Fang et al ., 2017 ) ."

eidos
"In addition , USP13 stabilizes microphthalmia-associated transcription factor and promotes cell invasion in melanoma [ 20 ] ; and , USP13 inhibition decreases proliferation of glioma stem cells by inducing c-Myc degradation [ 21 ] ."

reach
"Deubiquitinase USP13 promotes the epithelial-mesenchymal transition and metastasis in gastric cancer by maintaining Snail protein."

reach
"Furthermore, USP13 positively regulates the epithelial-mesenchymal transition (EMT) of GC cells."
| 2

reach
"USP13 knockdown prominently suppressed MGC-803 cell migration and invasion."

reach
"Finally, Snail knockdown significantly blocked USP13-induced SGC-7901 cell migration and invasion."
USP13 affects cell growth
| 2
USP13 inhibits cell growth.
| 1
| 1

reach
"3.2 Spautin-1, a dual USP10 and USP13 antagonist, inhibits melanoma cell growth in vitro."
| PMC
USP13 activates cell growth.
| 1
Mutated USP13 activates cell growth. 1 / 1
| 1

reach
"This enhancement was dependent on USP13 deubiquitinase activity, as a catalytically inactive mutant of USP13 (USP13 C345A) failed to increase cell growth."
USP13 affects cell death
| 2
| 2

reach
"Our study provides a potential therapeutic strategy in which targeting USP13 blocks biosynthesis of metabolic intermediates and lipids thereby simultaneously inducing energy stress and cell death."

reach
"Similar to Beclin 1 knockdown, we found that suppression of the ubiquitin specific peptidase, USP10, or a small molecule inhibitor of the deubiquitinases USP10 and USP13, i.e., spautin-1 XREF_BIBR, can increase radiation induced DSBs and promote tumor cell death."
USP13 affects ZEB1
2 |
2 |

No evidence text available

No evidence text available
| 2

eidos
"Our results demonstrate that USP13 is hijacked to maintain nsp13 expression and the inhibitory role of nsp13 in regulating type I IFN production , and USP13 inhibitor could be employed to suppress virus replication by targeting nsp13 for degradation thereby disrupting its inhibitory role in regulating type I IFN production ."

eidos
"Moreover , depletion of USP13 or treatment with USP13 inhibitor relieves the inhibitory role of nsp13 for type I IFN response and suppresses virus replication in host cells , suggesting that USP13 inhibitor could be employed to suppress virus replication by targeting nsp13 for degradation ."
USP13 affects Ufd1
| 2
| 2

sparser
"It was shown that USP13 associates with p97/VCP, Ufd1, Npl4, and UBXD8, and that depletion of USP13 leads to the accumulation of ERAD substrates [ xref ]."

sparser
"USP13 interacts with p97/VCP, Ufd1, Npl4, and UBXD8 [ xref ]."
USP13 affects USP5
1 | 1
GST binds USP13, USP5, and VCP. 1 / 1
| 1

sparser
"By contrast, neither GST-USP13 nor GST-USP5 bound p97 ( xref )."
1 |

No evidence text available
USP13 affects UBL
| 2
USP13 binds BAG6 and UBL. 2 / 2
| 2

sparser
"On the other hand, the binding of USP13 with the UBL domain of Bag6 can be demonstrated using pulldown experiments ( xref )."

sparser
"Taken together, these results suggest a direct interaction between USP13 and Bag6 that is mediated by the Bag6 UBL domain."
USP13 affects TRIM55
1 1 |
1 1 |

No evidence text available

No evidence text available
USP13 affects TCRalpha
| 2
USP13 activates TCRalpha. 2 / 2
| 2

reach
"When the degradation of the model ERAD substrate TCRalpha was examined in cells overexpressing either USP13 or the USP13 variants, we found that wild-type USP13 significantly increased the half-life of TCRalpha (from 1.6 hr to 4.6 hr)."

reach
"Immunoblotting confirmed that USP13 depletion caused more TCRalpha to partition into the NP40-insoluble fractions (XREF_FIG) compared to control cells."
USP13 affects SMC1A
2 |
2 |

No evidence text available

No evidence text available
USP13 affects SCCPDH
2 |
2 |

No evidence text available

No evidence text available
USP13 affects RPP25
2 |
2 |

No evidence text available

No evidence text available
USP13 affects OTUD3
| 2
| 1

sparser
"PTEN and DUBs were readily co-immunoprecipitated with each other; however, USP11, USP13, and OTUD3 were not associated with each other (Supplementary Fig.  xref )."
USP13 binds OTUD3 and PTEN. 1 / 1
| 1

sparser
"USP7, a highly expressed DUB in prostate cancer and progressive multifocal leukoencephalopathy (PML), plays a direct role in PTEN deubiquitination and regulates its localization rather than protein stability. xref In addition, USP13 and OTUD3 can interact with PTEN and remove its polyubiquitin chain."
USP13 affects NFkappaB
| 1 1
| 1 1

eidos
"USP13 suppresses NF-kB to promote PTEN expression , resulting in antitumor activity [ 65 ] ."

reach
"USP13 suppresses NF-kB to promote PTEN expression, resulting in antitumor activity [XREF_BIBR]."
USP13 affects MYO15A
1 1 |
1 1 |

No evidence text available

No evidence text available
USP13 affects MRPL20
1 1 |
1 1 |

No evidence text available

No evidence text available
USP13 affects MITF-M protein
| 2
USP13 activates MITF-M protein. 2 / 2
| 2

eidos
"Conversely , USP13 upregulation promoted MITF-M protein levels , without influencing MITF-M mRNA levels ."

eidos
"Furthermore , Exogenous USP13 significantly upregulated the endogenous MITF-M protein level , downregulated USP13 significantly inhibited MITF-M protein levels , without altering MITF-M mRNA expression ."
USP13 affects METTL3
| 2
USP13 binds METTL3.
| 1
USP13 binds METTL3 and K48. 1 / 1
| 1

reach
"USP13 interacts with, deubiquitinates, and therefore stabilizes METTL3 at K488 by removing K48-linked ubiquitin chains."
USP13 activates METTL3.
| 1
USP13 activates METTL3. 1 / 1
| 1

reach
"Collectively, our study results indicate that USP13 promotes glycolysis and tumor progression in OS by stabilizing METTL3, thereby stabilizing ATG5 mRNA and facilitating autophagy in OS."
USP13 affects LAPTM5
1 1 |
1 1 |

No evidence text available

No evidence text available
USP13 affects IRF3
| 2
USP13 phosphorylates IRF3.
| 1
USP13 leads to the phosphorylation of IRF3. 1 / 1
| 1

reach
"As shown in XREF_FIG, HSV-1- but not SeV induced phosphorylation of IRF3 or IkappaBalpha was impaired by reconstitution of USP13 but not USP13 (AE) into Usp13 m/m MEFs."
USP13 binds IRF3.
| 1
| 1

reach
"Although USP13 interacted with cGAS and IRF3 in an overexpression system, USP13 did not affect the ubiquitination of cGAS or IRF3 when coexpressed in HEK293 cells (XREF_SUPPLEMENTARY)."
USP13 affects FUNDC2
1 1 |
1 1 |

No evidence text available

No evidence text available
USP13 affects FOXO1/3
| 2
USP13 phosphorylates FOXO1/3.
| 1
USP13 leads to the phosphorylation of FOXO1/3. 1 / 1
| 1

reach
"Similarly, depletion of USP13 downregulated PTEN protein and upregulated AKT and FOXO1/3 phosphorylation in MCF10A (XREF_FIG) and MCF7 (XREF_FIG) mammary epithelial cells and in HCT116 colon cancer cells (XREF_FIG), but not in the isogenic PTEN-null HCT116 cells (XREF_FIG)."
USP13 dephosphorylates FOXO1/3.
| 1
Modified USP13 leads to the dephosphorylation of FOXO1/3. 1 / 1
| 1

reach
"This overexpression upregulated PTEN protein and downregulated AKT and FOXO1/3 phosphorylation in the MDAMB-231 cell line (XREF_FIG) which expresses moderate but detectable levels of endogenous PTEN (XREF_SUPPLEMENTARY), while knockdown of PTEN in USP13 overexpressing MDA-MB-231 cells rescued the phosphorylation of both AKT and FOXO1/3 (XREF_SUPPLEMENTARY)."
USP13 affects FNBP1L
1 1 |
1 1 |

No evidence text available

No evidence text available
| 2
| 2

reach
"Thus, several ubiquitin-conjugating E2 enzymes (UBE2L3, UBE2L5), the E3 ubiquitin ligases (TRIM21, TRIM38, ARIH2) and the ubiquitin specific protease or deconjugases (USP13) are 1.5-3.2-fold induced in the infected cells, while other E2, E3 enzymes and deconjugases (e.g."
| DOI

reach
"In melanoma cells, USP13 attenuates the autodegradation of Siah2, a RING (really interesting new gene) finger family E3 ubiquitin ligase."
USP13 affects DVL1
1 1 |
1 1 |

No evidence text available

No evidence text available
USP13 affects Cyclin
| 2
USP13 deubiquitinates Cyclin on D1. 2 / 2
| 2

reach
"USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation."

reach
"Taken together, our data demonstrate that USP13 deubiquitinates and stabilizes cyclin D1, thereby promoting cell cycle progression and cell proliferation in GC."
USP13 affects CLK3
| 1 1
| 1 1

reach
"A pulldown experiment suggests a direct binding of USP13 to CLK3 (XREF_FIG)."

sparser
"A pulldown experiment suggests a direct binding of USP13 to CLK3 ( xref )."
USP13 affects ATXN3
1 |
1 |

No evidence text available
USP13 affects ATG5
| 2
| 2

sparser
"Activation of PAK1 promoted phosphorylation of ATG5 thereby enhancing the interaction of ATG5 with USP13."

sparser
"USP13 preferentially is induced in GIST cells by IM and interacts with ATG5, which leads to stabilization of ATG5 through deubiquitination."
USP13 affects 7H-purine
| 2
| 2

reach
"CLK3 dependent phosphorylation of USP13 at Y708 promotes CCA progression by activating c-Myc-mediated purine synthesis."

reach
"Therefore, we propose that CLK3 induced Y708 phosphorylation of USP13 promotes CCA progression by activating c-Myc-mediated purine synthesis."

reach
"Conversely, USP13 overexpression in NCI-H2170 cells modestly impaired the inhibitory effects of ABT-263."

reach
"As anticipated, USP13 knockdown by siRNAs or knockout by CRISPR and Cas9 increased sensitivity to ABT-263 in SW-1573 and TOV-21G cells."
USP10 affects BECN1
| 2
| 2

sparser
"To determine the mechanism by which Vps34 complexes regulate the stability of USP13 and USP10, we examined the possibility that Beclin1 may interact with USP10 and USP13."

sparser
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."
UBL affects USP13
| 2
USP13 binds BAG6 and UBL. 2 / 2
| 2

sparser
"On the other hand, the binding of USP13 with the UBL domain of Bag6 can be demonstrated using pulldown experiments ( xref )."

sparser
"Taken together, these results suggest a direct interaction between USP13 and Bag6 that is mediated by the Bag6 UBL domain."
TRIM55 affects USP13
1 1 |
1 1 |

No evidence text available

No evidence text available
SMC3 affects USP13
2 |
2 |

No evidence text available

No evidence text available
SMC1A affects USP13
2 |
2 |

No evidence text available

No evidence text available
SCCPDH affects USP13
2 |
2 |

No evidence text available

No evidence text available
RPP25 affects USP13
2 |
2 |

No evidence text available

No evidence text available
OTUD3 affects USP13
| 2
| 1

sparser
"PTEN and DUBs were readily co-immunoprecipitated with each other; however, USP11, USP13, and OTUD3 were not associated with each other (Supplementary Fig.  xref )."
USP13 binds OTUD3 and PTEN. 1 / 1
| 1

sparser
"USP7, a highly expressed DUB in prostate cancer and progressive multifocal leukoencephalopathy (PML), plays a direct role in PTEN deubiquitination and regulates its localization rather than protein stability. xref In addition, USP13 and OTUD3 can interact with PTEN and remove its polyubiquitin chain."
OGDH affects ACLY
| 2
ACLY binds USP13 and OGDH. 2 / 2
| 2

sparser
"Reciprocal analyses in which immunoprecipitated ACLY or OGDH were probed for USP13 confirmed the USP13ACLY and USP13OGDH interactions ( xref )."

sparser
"Western blot analyses of HEK293T cell lysates immunoprecipitated with USP13 antibody showed the interaction of USP13 with ACLY and OGDH ( xref )."
NPLOC4 affects Ufd1
| 2
| 2

sparser
"It was shown that USP13 associates with p97/VCP, Ufd1, Npl4, and UBXD8, and that depletion of USP13 leads to the accumulation of ERAD substrates [ xref ]."

sparser
"USP13 interacts with p97/VCP, Ufd1, Npl4, and UBXD8 [ xref ]."
NFkappaB affects USP13
| 2
NFkappaB decreases the amount of USP13.
| 1
NFkappaB decreases the amount of USP13. 1 / 1
| 1

reach
"Our data demonstrated that NF-kB activation decreased expression level of USP13 and PTEN, and promoted the tumorigenesis phenotypes of BC cells."
NFkappaB activates USP13.
| 1
| 1

reach
"Thus, we sought to find if USP13 could be targeted by NF-kB and miR-130b ~ 301b signaling and further facilitate the loss of PTEN."
NFE2 affects USP13
2 |
NFE2 decreases the amount of USP13. 2 / 2
2 |

No evidence text available

No evidence text available
MYO15A affects USP13
1 1 |
1 1 |

No evidence text available

No evidence text available
MRPL20 affects USP13
1 1 |
1 1 |

No evidence text available

No evidence text available
MEF2A affects USP13
2 |
MEF2A decreases the amount of USP13. 2 / 2
2 |

No evidence text available

No evidence text available
LAPTM5 affects USP13
1 1 |
1 1 |

No evidence text available

No evidence text available
GST affects VCP
| 2
GST binds USP13 and VCP. 1 / 1
| 1

sparser
"We expressed these proteins in cells individually and examined the binding of endogenous p97 to GST-USP13."
GST binds USP13, USP5, and VCP. 1 / 1
| 1

sparser
"By contrast, neither GST-USP13 nor GST-USP5 bound p97 ( xref )."
FUNDC2 affects USP13
1 1 |
1 1 |

No evidence text available

No evidence text available
FNBP1L affects USP13
1 1 |
1 1 |

No evidence text available

No evidence text available
FAF2 affects Ufd1
| 2
| 2

sparser
"It was shown that USP13 associates with p97/VCP, Ufd1, Npl4, and UBXD8, and that depletion of USP13 leads to the accumulation of ERAD substrates [ xref ]."

sparser
"USP13 interacts with p97/VCP, Ufd1, Npl4, and UBXD8 [ xref ]."
DVL1 affects USP13
1 1 |
1 1 |

No evidence text available

No evidence text available
BECN1 affects USP10, and USP13
| 2
| 2

sparser
"To determine the mechanism by which Vps34 complexes regulate the stability of USP13 and USP10, we examined the possibility that Beclin1 may interact with USP10 and USP13."

sparser
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."
BAG6 affects UBL, and USP13
| 2
USP13 binds BAG6 and UBL. 2 / 2
| 2

sparser
"On the other hand, the binding of USP13 with the UBL domain of Bag6 can be demonstrated using pulldown experiments ( xref )."

sparser
"Taken together, these results suggest a direct interaction between USP13 and Bag6 that is mediated by the Bag6 UBL domain."
BAG6 affects AMFR
| 2
USP13 binds BAG6 and AMFR. 2 / 2
| 2

sparser
"USP13 forms a multiprotein complex with gp78 and Bag6."

sparser
"Since Bag6 can interact with both gp78 and USP13, we asked whether these proteins could form a multiprotein complex."
ATXN3 affects USP13
1 |
1 |

No evidence text available
ATG5 affects USP13
| 2
| 2

sparser
"Activation of PAK1 promoted phosphorylation of ATG5 thereby enhancing the interaction of ATG5 with USP13."

sparser
"USP13 preferentially is induced in GIST cells by IM and interacts with ATG5, which leads to stabilization of ATG5 through deubiquitination."
AMFR affects BAG6, and USP13
| 2
USP13 binds BAG6 and AMFR. 2 / 2
| 2

sparser
"USP13 forms a multiprotein complex with gp78 and Bag6."

sparser
"Since Bag6 can interact with both gp78 and USP13, we asked whether these proteins could form a multiprotein complex."
ACLY affects USP13
| 2
ACLY binds USP13 and OGDH. 2 / 2
| 2

sparser
"Reciprocal analyses in which immunoprecipitated ACLY or OGDH were probed for USP13 confirmed the USP13ACLY and USP13OGDH interactions ( xref )."

sparser
"Western blot analyses of HEK293T cell lysates immunoprecipitated with USP13 antibody showed the interaction of USP13 with ACLY and OGDH ( xref )."
Ubiquitin-like affects USP13
| 1
USP13 binds BAG6 and ubiquitin-like. 1 / 1
| 1

reach
"Indeed, GST pull-down experiments using purified proteins showed that GST-USP13 bound Bag6, and the N-terminal ubiquitin like (UBL) domain in Bag6 was both necessary and sufficient for interaction with USP13 (XREF_FIG)."
Ubiquitin E3 affects USP13
| 1
Ubiquitin E3 inhibits USP13. 1 / 1
| 1

reach
"The unidentified protein may be an ubiquitin E3 ligase or other mediator that promotes USP13 proteasomal degradation."
1 |
1 |

No evidence text available
Tetrachloromethane increases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Tamibarotene decreases the amount of USP13. 1 / 1
1 |

No evidence text available
Subsequent structural modification affects USP13
| 1
Subsequent structural modification inhibits USP13. 1 / 1
| 1

eidos
"USP10 / 13 Inhibitors Through an imaging-based screening and subsequent structural modification , a potent autophagy inhibitor spautin-1 ( Figure 19 ) was identified , which was demonstrated to inhibit USP10 and USP13 and thus promote the degradation of Vps34 PI3 kinase complexes [ 152 ] ."
Spautin-1.58 Spautin-1 affects USP13
| 1
Spautin-1.58 Spautin-1 inhibits USP13. 1 / 1
| 1

reach
"Next, we treated infected THP-1 cells with a cell-permeable potent autophagy inhibitor, spautin-1.58 Spautin-1 promotes the degradation of BECN1 by inhibiting 2 USPs (ubiquitin-specific peptidases), USP10 and USP13, which target BECN1.58 Spautin-1 treatment of infected cells significantly decreased E. chaffeensis proliferation (Fig. 3B)."

reach
"Future studies will be focused on examining ROS production and protein oxidation in USP13-deficient lungs and lung cells."
1 |
Pirinixic acid increases the amount of USP13. 1 / 1
1 |

No evidence text available
Nsp13 affects USP13
| 1
| 1

reach
"We found that nsp13 interacts with the deubiquitinase USP13, which deubiquitinates and stabilizes nsp13."
1 |
Nickel monoxide decreases the amount of USP13. 1 / 1
1 |

No evidence text available
MiR-145-5p affects USP13
| 1
MiR-145-5p inhibits USP13. 1 / 1
| 1

reach
"MicroRNA profiling reveals miR-145-5p inhibits goat myoblast differentiation by targeting the coding domain sequence of USP13."
MiR-130b~301b affects USP13
| 1
MiR-130b~301b activates USP13. 1 / 1
| 1

reach
"Thus, we sought to find if USP13 could be targeted by NF-kB and miR-130b ~ 301b signaling and further facilitate the loss of PTEN."
Isobutanol affects USP13
1 |
Isobutanol increases the amount of USP13. 1 / 1
1 |

No evidence text available
Indometacin affects USP13
1 |
Indometacin increases the amount of USP13. 1 / 1
1 |

No evidence text available
Imaging-based screening affects USP13
| 1
Imaging-based screening inhibits USP13. 1 / 1
| 1

eidos
"USP10 / 13 Inhibitors Through an imaging-based screening and subsequent structural modification , a potent autophagy inhibitor spautin-1 ( Figure 19 ) was identified , which was demonstrated to inhibit USP10 and USP13 and thus promote the degradation of Vps34 PI3 kinase complexes [ 152 ] ."
1 |
Hsa-miR-6080 decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-548g-5p decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-548f-5p decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-548aj-5p decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-539-3p decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-4778-3p decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-4659a-3p decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-454-3p decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-4295 decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-3164 decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-3163 decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-3118 decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-301b-3p decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-1468-3p decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-134-5p decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-130b-3p decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-1305 decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Formaldehyde decreases the amount of USP13. 1 / 1
1 |

No evidence text available
Exposure LPS affects USP13
| 1
Exposure LPS activates USP13. 1 / 1
| 1

eidos
"We found that short time exposure of LPS did not trigger USP13 degradation , suggesting that an immediate LPS signaling through TLR4 does not contribute to USP13 ubiquitination and degradation ."
Endosulfan affects USP13
1 |
Endosulfan increases the amount of USP13. 1 / 1
1 |

No evidence text available
Diuron affects USP13
1 |
Diuron decreases the amount of USP13. 1 / 1
1 |

No evidence text available
Copper atom affects USP13
1 |
Copper atom decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Cefoperazone decreases the amount of USP13. 1 / 1
1 |

No evidence text available
Bortezomib affects USP13
| 1
| 1

reach
"Bortezomib treatment increased accumulation of USP13 (Fig. 4E), indicating that USP13 degradation is mediated by the ubiquitin-proteasome system."
Bisphenol A affects USP13
1 |
Bisphenol A decreases the amount of USP13. 1 / 1
1 |

No evidence text available

reach
"Another autophagy inhibitor, spautin-1, degrades class III PI3K by inhibiting the activity of the ubiquitin specific peptidases, USP10 and USP13 (Mateo et al., 2013, Liu et al., 2011)."
AurK affects USP13
| 1
| 1

sparser
"Overall our study unveils the molecular and cellular connections of the USP13-Aurora B axis, which potentially participates in the rewiring of the cell cycle happening in cancer cells."
1 |
Arsenic atom decreases the amount of USP13. 1 / 1
1 |

No evidence text available
All-trans-retinoic acid decreases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
1 |

No evidence text available
Acrylamide affects USP13
1 |
Acrylamide increases the amount of USP13. 1 / 1
1 |

No evidence text available
1 |
Zoledronic Acid decreases the amount of USP13. 1 / 1
1 |

No evidence text available
VCP affects USP5
| 1
GST binds USP13, USP5, and VCP. 1 / 1
| 1

sparser
"By contrast, neither GST-USP13 nor GST-USP5 bound p97 ( xref )."
VCP affects UFD1
| 1
| 1

sparser
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
VCP affects AMFR
| 1
USP13 binds VCP and AMFR. 1 / 1
| 1

sparser
"These results demonstrated a specific and direct interaction between USP13 and gp78 that facilitates USP13-p97 association."
USP5 affects VCP
| 1
GST binds USP13, USP5, and VCP. 1 / 1
| 1

sparser
"By contrast, neither GST-USP13 nor GST-USP5 bound p97 ( xref )."
USP39 affects USP13
| 1
| 1

sparser
"For example, the three deubiquitinating enzymes BRCC36, USP13, and USP39 interact with STAT1 and decrease the K63-, K48- and K6-linked polyubiquitin chains of STAT1 respectively (110, 115, 118)."
USP13 affects viruses caused nsp13 overexpression
| 1
USP13 activates viruses caused nsp13 overexpression. 1 / 1
| 1

eidos
"Depletion of USP13 led to the reduction of viruses caused by nsp13 overexpression ."
USP13 affects virus-induced
| 1
USP13 inhibits virus-induced. 1 / 1
| 1

eidos
"However , USP13 , USP19 , and USP22 inhibit virus-induced IFN production by removing K27-linked polyubiquitin chains from STING ( 40 , 81 ) or TRIF ( 90 ) ."
USP13 affects ubiquitination nsp13
| 1
USP13 inhibits ubiquitination nsp13. 1 / 1
| 1

eidos
"Loss of USP13 enhances ubiquitination of nsp13 and destabilizes nsp13 protein ."
USP13 affects ubiquitination TXN
| 1
USP13 inhibits ubiquitination TXN. 1 / 1
| 1

eidos
"Here , we found that knockdown of USP13 increased the ubiquitination of TXN , as well as the activation of CASP3 , suggesting that ubiquitination of TXN is related to the activation of CASP3 ."
USP13 affects ubiquitinated nsp13
| 1
USP13 inhibits ubiquitinated nsp13. 1 / 1
| 1

eidos
"Loss of USP13 led to the increase of ubiquitinated nsp13 ( Supplementary Fig. S3g ) ."
USP13 affects ubiquitinated Beclin1
| 1
Modified USP13 decreases the amount of ubiquitinated Beclin1. 1 / 1
| 1

reach
"Similarly, the overexpression of USP13 reduced the levels of ubiquitinated Beclin1 which was inhibited by spautin-1 (XREF_FIG)."
USP13 affects ubiquitin-mediated mutant EGFR
| 1
USP13 inhibits ubiquitin-mediated mutant EGFR. 1 / 1
| 1

eidos
"USP13 inhibits the ubiquitin-mediated degradation of mutant EGFR To understand the interplay between USP13 and mutant EGFR proteins , we first analyzed their respective subcellular localization ."
USP13 affects ubiquitin-mediated TLR4
| 1
USP13 inhibits ubiquitin-mediated TLR4. 1 / 1
| 1

eidos
"USP13 interacted with TLR4 and inhibited the ubiquitin-mediated degradation of TLR4 ."
USP13 affects ubiquitin-like
| 1
USP13 binds BAG6 and ubiquitin-like. 1 / 1
| 1

reach
"Indeed, GST pull-down experiments using purified proteins showed that GST-USP13 bound Bag6, and the N-terminal ubiquitin like (UBL) domain in Bag6 was both necessary and sufficient for interaction with USP13 (XREF_FIG)."

reach
"Overexpression of USP13 inhibited wild-type and K63R mutant forms of ubiquitin associated ubiquitination of ACLY, but had no effect on the K48R ubiquitin associated ubiquitination (XREF_FIG)."
USP13 affects tumorigenic metastatic primary murine ovarian cancer cells
| 1
USP13 activates tumorigenic metastatic primary murine ovarian cancer cells. 1 / 1
| 1

eidos
"USP13 also increased the tumorigenic and metastatic abilities of primary murine ovarian cancer cells in a syngeneic mouse study ."
USP13 affects tumor initiation
| 1
USP13 activates tumor initiation. 1 / 1
| 1

eidos
"Some findings suggest that USP13 may promote tumor initiation or progression ."
USP13 affects synovial hyperplasia inflammatory cell infiltration cartilage bone collagen-induced arthritis
| 1
USP13 inhibits synovial hyperplasia inflammatory cell infiltration cartilage bone collagen-induced arthritis. 1 / 1
| 1

eidos
"The in vivo experiments finally confirmed that USP13 dramatically repressed synovial hyperplasia , inflammatory cell infiltration , cartilage damage and bone loss in collagen-induced arthritis ( CIA ) mice via the same molecular mechanisms detected in vitro ."
USP13 affects survival lung cancer cells treated afatinib
| 1
USP13 activates survival lung cancer cells treated afatinib. 1 / 1
| 1

eidos
"Accordingly , inhibition of USP13 led to a significant decrease in the survival of lung cancer cells treated with afatinib in vitro and in vivo ."
USP13 affects stability C adaptor CDH1
| 1
USP13 activates stability C adaptor CDH1. 1 / 1
| 1

eidos
"USP13 modulates the stability of the APC / C adaptor CDH1 ."
USP13 affects stability ACLY
| 1
USP13 activates stability ACLY. 1 / 1
| 1

eidos
"However , USP13 and USP30 can mediate deubiquitination of ACLY , increasing the stability of ACLY to promote development of ovarian cancer and hepatocellular carcinoma , respectively [ 271 , 279 ] ."
| PMC
USP13 affects signaling
| 1
USP13 inhibits signaling. 1 / 1
| 1

sparser
"We conclude from these data that USP13 inhibits AKT signaling through positive regulation of PTEN protein."
USP13 affects sensitivity cervical cancer cells BH3 mimetic ABT-263
| 1
USP13 activates sensitivity cervical cancer cells BH3 mimetic ABT-263. 1 / 1
| 1

eidos
"Finally , we demonstrated that depletion of USP13 expression or inhibition of USP13 enzymatic activity increased the sensitivity of cervical cancer cells to the BH3 mimetic inhibitor ABT-263 ."
USP13 affects retention ac-SMC3 chromatin
| 1
USP13 inhibits retention ac-SMC3 chromatin. 1 / 1
| 1

eidos
"Inactivation of USP13 resulted in the retention of ac-SMC3 on chromatin in mitosis ( Fig. 8B ) ."
USP13 affects replication stress response
| 1
USP13 activates replication stress response. 1 / 1
| 1

eidos
"Furthermore , high USP13 expression enhances the replication stress response , promotes cancer cell chemoresistance , and is correlated with poor prognosis of cancer patients ."

reach
"Similarly, USP10 and USP13 silencing could induce ROS generation and cause DNA damage."
| PMC
USP13 affects production
| 1
USP13 inhibits production. 1 / 1
| 1

sparser
"In support of the involvement of USP13 in bioenergetics, USP13-KD inhibited ATP production in cells ( xref )."
| 1
| 1

reach
"Our future study will explore whether the USP13 contributes to the pathogenesis of lung fibrosis."
USP13 affects p-RPA32 S33 staining
| 1
USP13 activates p-RPA32 S33 staining. 1 / 1
| 1

eidos
"As shown in Fig. 3A-B and Fig. S2 , depletion of USP13 diminished p-RPA32 ( S33 ) staining after hydroxyurea ( HU ) treatment ."
USP13 affects ovarian cancer cell
| 1
USP13 activates ovarian cancer cell. 1 / 1
| 1

eidos
"A previous study reports that USP13 knockdown suppresses ovarian cancer cell proliferation in vitro and tumor formation in vivo ( Han et al ., 2016 ) ."
USP13 affects olaparib
| 1
| 1

reach
"In addition, reconstitution of WT USP13, but not the T196A mutant, fully rescued the foci formation of the BRCA1-A complex, DNA repair and cell cycle checkpoint in USP13 deficient cells and reversed hypersensitivity to cisplatin or olaparib induced by USP13 deficiency (XREF_FIG and XREF_SUPPLEMENTARY)."

reach
"N6-methyladenosine-modified USP13 induces pro-survival autophagy and imatinib resistance via regulating the stabilization of autophagy-related protein 5 in gastrointestinal stromal tumors."
USP13 affects needs be determined
| 1
USP13 activates needs be determined. 1 / 1
| 1

eidos
"The effect of USP13 on anti-inflammatory receptor Sigirr 's or other anti-inflammatory mediators ' stability in Kupffer cells needs to be determined in future studies ."
USP13 affects miR-130b-3p
| 1
USP13 activates miR-130b-3p. 1 / 1
| 1

reach
"Furthermore, we discovered that USP13 was a common target of miR-130b-3p and miR-301b-3p, and the miR-130b/301b cluster, which could be transcriptionally upregulated by NF-kB."
USP13 affects membrane BAG6 cleavage
| 1
USP13 activates membrane BAG6 cleavage. 1 / 1
| 1

eidos
"( F ) Knockdown of CASP3 inhibits membrane fraction BAG6 cleavage , which is induced by USP13 knockdown under MG132 treatment ."
USP13 affects maturation
| 1
USP13 activates maturation. 1 / 1
| 1

eidos
"Spautin-1 reportedly inhibits autophagosomal initiation and maturation by inhibiting deubiquitylases USP10 and USP13 , which stabilize the Beclin-1 / VPS34 complex ( 26 ) ."
USP13 affects lyase
| 1
USP13 activates lyase. 1 / 1
| 1

reach
"USP13 specifically deubiquitinates and thus upregulates ATP citrate lyase and oxoglutarate dehydrogenase, two key enzymes that determine mitochondrial respiration, glutaminolysis and fatty acid synthesis."
USP13 affects lung metastasis
| 1
USP13 activates lung metastasis. 1 / 1
| 1

eidos
"H & E staining of lung tissues suggested that USP13 knockdown reduced lung metastasis of HCC in mice ."
USP13 affects lung inflammation
| 1
USP13 inhibits lung inflammation. 1 / 1
| 1

eidos
"We have shown that USP13 deficiency promotes lung inflammation ."
USP13 affects lung cancer cells
| 1
USP13 activates lung cancer cells. 1 / 1
| 1

eidos
"37 USP13 is likely to stabilize mutant EGFR in a ubiquitinated state , and inhibition of USP13 destabilizes mutant EGFR and abrogates signaling of mutant EGFR in lung cancer cells ."
USP13 affects involvement development
| 1
USP13 activates involvement development. 1 / 1
| 1

eidos
"Deubiquitinase USP13 deficiency prevented the growth of melanoma in vitro and in vivo ; however , MITF overexpression prevented USP13 's inhibition , indicating MITF 's involvement in melanoma development , which was mediated by USP13 ( Zhao et al ., 2011 ) ."
USP13 affects increased lung inflammation enlargement alveolar spaces response CS exposure
| 1
USP13 inhibits increased lung inflammation enlargement alveolar spaces response CS exposure. 1 / 1
| 1

eidos
"We used a mild CS exposure system and found that USP13 deficiency caused loss of extracellular matrix , increased lung inflammation and enlargement of alveolar spaces in response to CS exposure ."
USP13 affects hypoxia-induced activation TLR4 MyD88 NF-kappaB pathway
| 1
USP13 activates hypoxia-induced activation TLR4 MyD88 NF-kappaB pathway. 1 / 1
| 1

eidos
"Hypoxia Activates the TLR4 / MyD88 / NF-kappaB Pathway via Inducing USP13 in HCC Cells Since hypoxia has been recognized as an inducer of the activation of the TLR4 / MyD88 / NF-kappaB pathway in HCC ( Won et al ., 2015 ; Zhang et al ., 2016 ) , therefore , we aimed to investigate whether USP13 mediated the hypoxia-induced activation of the TLR4 / MyD88 / NF-kappaB pathway in HCC ."
USP13 affects hypersensitizes cells
| 1
USP13 activates hypersensitizes cells. 1 / 1
| 1

eidos
"Depletion of USP13 impedes ATR activation and hypersensitizes cells to replication stress-inducing agents ."
USP13 affects half-life mutant protein NSCLC cells
| 1
USP13 activates half-life mutant protein NSCLC cells. 1 / 1
| 1

eidos
"Moreover , USP13 inhibition and silencing lead to a decreased half-life of the mutant EGFR protein in NSCLC cells , indicating that USP13 likely stabilizes EGFR ( Figure 3D ) ."
USP13 affects growth metastasis mice
| 1
USP13 activates growth metastasis mice. 1 / 1
| 1

eidos
"USP13 knockdown inhibits HCC growth and metastasis in mice ."
USP13 affects greater ubiquitinated nsp13
| 1
USP13 inhibits greater ubiquitinated nsp13. 1 / 1
| 1

eidos
"Predicting hijacking of host de-ubiquitination by the virus , Guo et al. checked for interactions between host de-ubiquitinase and nsp13 and found USP13 to interact with it and observed that loss of USP13 led to greater ubiquitinated nsp13 ."
| 1
| 1

reach
"In line with our hypothesis, we found that USP13-KD significantly decreased M5 citrate, M3 fumarate and M3 malate pools in CAOV3 cells (XREF_FIG, top panel), whereas overexpression of USP13 had the opposite effect in SKOV3 cells (XREF_FIG, bottom panel)."
USP13 affects fibronectin lung fibroblast cells
| 1
USP13 activates fibronectin lung fibroblast cells. 1 / 1
| 1

eidos
"Our previous study showed that USP13 reduction decreased fibronectin expression in lung fibroblast cells [ 18 ] ."
USP13 affects fast migrating BAG6 species
| 1
USP13 activates fast migrating BAG6 species. 1 / 1
| 1

eidos
"As expected , knockdown of USP13 generated a fast migrating BAG6 species , which was more accumulated in the presence of MG132 but was blocked with the treatment of a pan-caspase inhibitor Z-VAD-FMK ( Figure 1E ) ."
USP13 affects early autophagy
| 1
USP13 activates early autophagy. 1 / 1
| 1

eidos
"These data suggest that USP13 knockdown may suppress the early step of autophagy ."
USP13 affects development metastasis high-grade serous ovarian carcinoma
| 1
USP13 activates development metastasis high-grade serous ovarian carcinoma. 1 / 1
| 1

eidos
"USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model ."
USP13 affects development cervical cancer
| 1
USP13 activates development cervical cancer. 1 / 1
| 1

eidos
"To investigate if USP13 expression may contribute to the development of cervical cancer , we analysed USP13 mRNA expression in cervical cytology samples from a cohort of HPV16 + patients ."
USP13 affects deubiquitination ACLY
| 1
USP13 activates deubiquitination ACLY. 1 / 1
| 1

eidos
"However , USP13 and USP30 can mediate deubiquitination of ACLY , increasing the stability of ACLY to promote development of ovarian cancer and hepatocellular carcinoma , respectively [ 271 , 279 ] ."
| PMC
USP13 affects colony
| 1
USP13 activates colony. 1 / 1
| 1

eidos
"The absence of USP13 also led to a reduction in colony formation under anchorage dependent ( Fig. 3C ) and independent conditions ( Fig. 3D ) ."
USP13 affects cigarette smoke-induced alveolar space enlargement
| 1
USP13 inhibits cigarette smoke-induced alveolar space enlargement. 1 / 1
| 1

eidos
"USP13 deficiency aggravates cigarette smoke-induced alveolar space enlargement through stabilization of TXNIP ."
USP13 affects cervical cancer cell
| 1
USP13 activates cervical cancer cell. 1 / 1
| 1

eidos
"Depletion of USP13 inhibited cervical cancer cell proliferation ."

eidos
"USP13 deubiquitinates receptor-associated protein 80 ( RAP80 ) and promotes DNA damage response ."
USP13 affects cell unstressed cells
| 1
USP13 activates cell unstressed cells. 1 / 1
| 1

eidos
"In lung and ovarian cancers , where USP13 also regulates Mcl-1 stability , USP13 does not contribute to the cell proliferation of unstressed cells ( Fig. 3 ) ."
| 1

reach
"Furthermore, re-expression of cyclin D1 partially reversed the cell cycle arrest and cell proliferation inhibition induced by USP13 depletion in GC cells."
USP13 affects caused nsp13 overexpression
| 1
USP13 activates caused nsp13 overexpression. 1 / 1
| 1

eidos
"As shown in Supplementary Fig. S4a , nsp13 overexpression led to decrease of IFN-beta levels , and loss of USP13 can reverse the decrease of IFN-beta levels caused by nsp13 overexpression ."
USP13 affects caspase activation
| 1
USP13 inhibits caspase activation. 1 / 1
| 1

eidos
"Depletion of USP13 leads to caspase activation and cleavage of the ERAD chaperone BAG6 , which is reversed by knockdown of AMFR ."
USP13 affects cancer cell chemoresistance
| 1
USP13 activates cancer cell chemoresistance. 1 / 1
| 1

eidos
"Furthermore , high USP13 expression enhances the replication stress response , promotes cancer cell chemoresistance , and is correlated with poor prognosis of cancer patients ."
USP13 affects bind
| 1
USP13 activates bind. 1 / 1
| 1

eidos
"The knockdown of USP13 dramatically diminishes cisplatin-induced RAP80-BRCA1 foci formation , for its deficiency decreases the bind between RAP80 and K63-linked ubiquitin chain and fail the BRCA1 recruitment ( Li et al ., 2017b ) ."
USP13 affects autodegradation E3 ubiquitin ligase SIAH2
| 1
USP13 inhibits autodegradation E3 ubiquitin ligase SIAH2. 1 / 1
| 1

eidos
"Since USP13 limits the autodegradation of the E3 ubiquitin ligase SIAH2 , decreased USP13 expression seen under hypoxia increased SIAH2 activity against its target substrates Sprouty2 and PHD3 [ 136 ] ."
USP13 affects aurK
| 1
| 1

sparser
"Overall our study unveils the molecular and cellular connections of the USP13-Aurora B axis, which potentially participates in the rewiring of the cell cycle happening in cancer cells."
USP13 affects association
| 1
USP13 inhibits association. 1 / 1
| 1

sparser
"Interestingly, overexpression of USP13 inhibited TBK1-STING association but not TBK1-MAVS association ( xref )."
USP13 affects antiviral responses
| 1
USP13 inhibits antiviral responses. 1 / 1
| 1

eidos
"Nevertheless , USP13 deficiency enhances antiviral responses through deubiquitinating stimulator of interferon ( STING ) [ 56 ] ."
USP13 affects active caspase
| 1
USP13 inhibits active caspase. 1 / 1
| 1

eidos
"The downregulation of USP13 also increased active caspase 3 , indicating tumor apoptosis ( Figure 5A ) ."
USP13 affects acetyl-CoA
| 1
| 1

reach
"Inhibition of USP13 simultaneously suppresses glutamate anaplerosis to refill the TCA cycle and the generation of acetyl-CoA, a vital building block for de novo biosynthesis of fatty acids, leading to the marked supersession of OVCA cell proliferation and tumourigenic potential."
USP13 affects accumulation ERAD substrates
| 1
USP13 inhibits accumulation ERAD substrates. 1 / 1
| 1

eidos
"Interestingly , the depletion of USP13 leads to the accumulation of ERAD substrates ."
USP13 affects abundance anti-apoptotic protein MCL1 lung
| 1
USP13 activates abundance anti-apoptotic protein MCL1 lung. 1 / 1
| 1

eidos
"In lung infection , USP13 are aberrantly expressed , inhibition of USP13 reduces the abundance of anti-apoptotic protein MCL1 in the lung [ 42 ] ."
USP13 affects ZHX2 protein stability
| 1
USP13 activates ZHX2 protein stability. 1 / 1
| 1

eidos
"As a result , USP13 promoted ZHX2 protein stability in an enzymatically dependent manner , and depletion of USP13 led to ZHX2 down-regulation in ccRCC ."
USP13 affects USP39
| 1
| 1

sparser
"For example, the three deubiquitinating enzymes BRCC36, USP13, and USP39 interact with STAT1 and decrease the K63-, K48- and K6-linked polyubiquitin chains of STAT1 respectively (110, 115, 118)."
USP13 affects USP11
| 1
| 1

sparser
"PTEN and DUBs were readily co-immunoprecipitated with each other; however, USP11, USP13, and OTUD3 were not associated with each other (Supplementary Fig.  xref )."
USP13 affects UBE2I
| 1
UBE2I binds USP13 and MuRF. 1 / 1
| 1

sparser
"MURF family members interact with Ubc9 and ISOT-3, but only MURF-1 interacts with the transcriptional modulator GMEB-1."
USP13 affects Thr122
| 1
CK2 binds USP13 and Thr122. 1 / 1
| 1

reach
"We further demonstrated that Casein kinase 2 (CK2) directly interacts with and phosphorylates USP13 at Thr122, which promotes the stability of USP13 protein."
USP13 affects TXNIP lungs lung fibroblast cells
| 1
USP13 activates TXNIP lungs lung fibroblast cells. 1 / 1
| 1

eidos
"Knockdown of USP13 significantly reduced TXNIP levels in lungs or lung fibroblast cells ."
USP13 affects TRIM69
| 1
USP13 activates TRIM69. 1 / 1
| 1

reach
"However, USP13, USP19, and USP22 inhibit virus-induced IFN production by removing K27-linked polyubiquitin chains from STING (40, 81) or TRIF (90)."
USP13 affects TNF-alpha induction
| 1
USP13 activates TNF-alpha induction. 1 / 1
| 1

eidos
"An inhibitor of USP13 decreased the K63 polyubiquitination on TRAF6 , TAK1 phosphorylation , IL-1beta , and TNF-alpha induction in response to LPS in BMDMs ."
USP13 affects TNF
| 1
USP13 increases the amount of TNF. 1 / 1
| 1

reach
"Knockdown of USP13 by transiently transfecting with siUSP13 significantly potentiated expression of HSV-1-, but not SeV triggered expression of IFNB and TNFA in THP-1 cells (XREF_SUPPLEMENTARY)."
USP13 affects TLR4 abundance
| 1
USP13 activates TLR4 abundance. 1 / 1
| 1

eidos
"These results indicated that USP13 increased TLR4 abundance via enhancing the deubiquitination of TLR4 in HCC cells ."
USP13 affects THOC1
1 |
1 |

No evidence text available
USP13 affects TCRalpha-YFP
| 1
USP13 increases the amount of TCRalpha-YFP. 1 / 1
| 1

reach
"USP13 depletion significantly increased the levels of TCRalpha-YFP, as revealed by both immunoblotting and flow cytometry analyses (XREF_FIG)."
USP13 affects TCAF1
1 |
1 |

No evidence text available
USP13 affects TBK1
| 1
| 1

sparser
"In support of this notion, we observed that (i) USP13 deficiency in mice or knockdown of USP13 in human cells potentiated HSV-1-triggered activation of IRF3 and NF-κB and subsequent induction of type I IFNs and proinflammatory cytokines, (ii) USP13 deficient cells restricted HSV-1 replication more profoundly than did the wild-type controls and USP13 deficient mice were more resistant to lethal HSV-1 infection compared to the wild-type littermates, (iii) USP13 but not USP13(AE) removed K27-linked ubiquitination of STING in cells or in vitro , and (iv) overexpression of USP13 specifically disrupted TBK1-STING association and knockdown or knockout of USP13 potentiated STING-TBK1 association after HSV-1 infection."
USP13 affects TBCB
| 1
USP13 binds BAG6 and TBCB. 1 / 1
| 1

sparser
"We next tested whether USP13 could physically interact with Bag6 and/or its cofactors."
USP13 affects TAK1 phosphorylation
| 1
USP13 activates TAK1 phosphorylation. 1 / 1
| 1

eidos
"An inhibitor of USP13 decreased the K63 polyubiquitination on TRAF6 , TAK1 phosphorylation , IL-1beta , and TNF-alpha induction in response to LPS in BMDMs ."
| 1
| 1

reach
"USP13 interacted with Snail to deubiquitinate and stabilize Snail in GC cells."
USP13 affects Smad4 half-life
| 1
USP13 activates Smad4 half-life. 1 / 1
| 1

eidos
"Knockdown of USP13 decreased Smad4 half-life and promoted Smad4 ubiquitination ."
| 1
| 1

eidos
"Overexpression of USP13 increased FN and Smad4 protein levels in lung fibroblasts , while downregulation of USP13 reduced Smad4 protein levels , without altering Smad4 mRNA expression , suggesting that USP13 regulates Smad4 protein stability ."
USP13 affects SiHa cells
| 1
USP13 inhibits SiHa cells. 1 / 1
| 1

eidos
"In both HeLa and SiHa cells , inhibition of USP13 by Spautin-1 resulted in a dose-dependent decrease in Mcl-1 protein expression ( Fig. 8A ) ."
USP13 affects STING-TBK1
| 1
USP13 activates STING-TBK1. 1 / 1
| 1

reach
"In support of this notion, we observed that (i) USP13 deficiency in mice or knockdown of USP13 in human cells potentiated HSV-1-triggered activation of IRF3 and NF-kappaB and subsequent induction of type I IFNs and proinflammatory cytokines, (ii) USP13 deficient cells restricted HSV-1 replication more profoundly than did the wild-type controls and USP13 deficient mice were more resistant to lethal HSV-1 infection compared to the wild-type littermates, (iii) USP13 but not USP13 (AE) removed K27 linked ubiquitination of STING in cells or in vitro, and (iv) overexpression of USP13 specifically disrupted TBK1 STING association and knockdown or knockout of USP13 potentiated STING-TBK1 association after HSV-1 infection."
USP13 affects SPRR2C
1 |
1 |

No evidence text available
USP13 affects SNCA
| 1
USP13 leads to the ubiquitination of SNCA. 1 / 1
| 1

reach
"USP13 inhibition in transgenic mutant alpha-synuclein (A53T) mice increased the ubiquitination of alpha-synuclein and reduced its protein levels."
USP13 affects RAD23A
1 |
1 |

No evidence text available
USP13 affects QHO60603
1 |
USP13 binds QHO60603. 1 / 1
1 |

No evidence text available
| DOI

sparser
"Like NLRP3, the NLRP3 NACHT domain and LRR domain interacted with USP13, but the NLRP3 PYRIN domain failed to interact with USP13 (Fig.  xref h)."
USP13 affects PTEN-null
| 1
USP13 inhibits PTEN-null. 1 / 1
| 1

reach
"Moreover, knockdown of USP13 promoted the proliferation of HCT116 colon cancer cells but not the isogenic PTEN-null HCT116 cells (XREF_FIG)."
USP13 affects PMS1
1 |
1 |

No evidence text available
USP13 affects PI3K
| 1
USP13 inhibits PI3K. 1 / 1
| 1

reach
"While A20 inhibits PtdIns3P signaling by removing the TRAF6 dependent Lys63 linked chains from Beclin 1, the enzymes USP10 and USP13 prevent PI3K and III complex components from their degradation and, therefore, support autophagy."
USP13 affects NFKBIA
| 1
USP13 leads to the phosphorylation of NFKBIA. 1 / 1
| 1

reach
"As shown in XREF_FIG, HSV-1- but not SeV induced phosphorylation of IRF3 or IkappaBalpha was impaired by reconstitution of USP13 but not USP13 (AE) into Usp13 m/m MEFs."
USP13 affects NEDD8
1 |
1 |

No evidence text available
USP13 affects MuRF
| 1
UBE2I binds USP13 and MuRF. 1 / 1
| 1

sparser
"MURF family members interact with Ubc9 and ISOT-3, but only MURF-1 interacts with the transcriptional modulator GMEB-1."
USP13 affects MOV10
1 |
1 |

No evidence text available
USP13 affects MGC-803 cell migration
| 1
USP13 activates MGC-803 cell migration. 1 / 1
| 1

eidos
"USP13 knockdown prominently suppressed MGC-803 cell migration and invasion ."
USP13 affects MAP1LC3B
| 1
| 1

sparser
"We also used an in vitro assay to test the effect of treating HA-Ub-conjugated FLAG-LC3B with recombinant His6-tagged USP10 (His6-USP10) or His6-tagged USP13 (His6-USP13) (specificity control)."
USP13 affects Lung
| 1
USP13 activates Lung. 1 / 1
| 1

eidos
"Taken together , USP13 deficiency causes loss in extracellular matrix levels in lungs , thereby enlarging alveolar space in response to CS exposure ."
USP13 affects KCTD3
1 |
1 |

No evidence text available
USP13 affects K63 polyubiquitination TRAF6
| 1
USP13 activates K63 polyubiquitination TRAF6. 1 / 1
| 1

eidos
"An inhibitor of USP13 decreased the K63 polyubiquitination on TRAF6 , TAK1 phosphorylation , IL-1beta , and TNF-alpha induction in response to LPS in BMDMs ."
USP13 affects K48
| 1
USP13 binds METTL3 and K48. 1 / 1
| 1

reach
"USP13 interacts with, deubiquitinates, and therefore stabilizes METTL3 at K488 by removing K48-linked ubiquitin chains."
USP13 affects ITCH
1 |
1 |

No evidence text available
USP13 affects IL6
| 1
USP13 inhibits IL6. 1 / 1
| 1

reach
"Both miR-19a-3p knockdown and USP13 overexpression in MH-S cells inhibited the LPS induced production of TNF-alpha, IL-6 and IL-1beta."
USP13 affects IL1B
| 1
USP13 inhibits IL1B. 1 / 1
| 1

reach
"Both miR-19a-3p knockdown and USP13 overexpression in MH-S cells inhibited the LPS induced production of TNF-alpha, IL-6 and IL-1beta."
USP13 affects IL-1R8
| 1
USP13 leads to the ubiquitination of IL-1R8. 1 / 1
| 1

reach
"Knockdown of USP13 in cells increased IL-1R8 and Sigirr poly-ubiquitination and reduced its stability, which enhanced LPS induced TLR4 signaling and cytokine release."
USP13 affects HMOX1 basal
| 1
USP13 inhibits HMOX1 basal. 1 / 1
| 1

eidos
"Knockdown of USP13 increased HMOX1 basal levels but did not rescue the HMOX1 reduction by TGF-beta1 ( Fig. 2B ) ."
USP13 affects HCC Cell Invasion Hep3B Huh7 cells expressed USP13 were transfected independent shRNAs USP13
| 1
USP13 activates HCC Cell Invasion Hep3B Huh7 cells expressed USP13 were transfected independent shRNAs USP13. 1 / 1
| 1

eidos
"USP13 Knockdown Suppresses HCC Cell Proliferation and Invasion in vitro and in vivo Hep3B and Huh7 cells , which expressed a relatively higher level of USP13 , were transfected with two independent shRNAs to specifically downregulate USP13 ( P < 0.05 , Figure 2A ) ."
USP13 affects GSDMD
| 1
USP13 activates GSDMD. 1 / 1
| 1

reach
"Overexpression of USP13 in PC12 cells almost reversed the Spautin‐1‐mediated decrease in NLRP3, USP13, Beclin1 and GSDMD‐N expression levels (Figure 3D,E)."
USP13 affects GP78-mediated ubiquitination UBL4A
| 1
USP13 inhibits GP78-mediated ubiquitination UBL4A. 1 / 1
| 1

eidos
"These data suggested that USP13 prevented GP78-mediated ubiquitination of UBL4A by deubiquitination and stabilized the expression of ERAD mechanical protein Ubl4A , thus playing a positive role in the degradation of misfolded proteins ( Figure 2k ) ."
USP13 affects GET4
1 |
1 |

No evidence text available
USP13 affects GEMIN4
| 1
| 1

reach
"In addition, USP13 interacts with the P97 and valosin containing protein complex, a key chaperone in ER associated degradation, which increases the protein level of CD3delta, an ER associated degradation substrate."
USP13 affects FASN
| 1
USP13 activates FASN. 1 / 1
| 1

reach
"Mechanistically, USP13 interacts with fatty acid synthase (FASN) and enhances FASN protein stability."
USP13 affects DNA Damage
| 1
| 1

reach
"Similarly, USP10 and USP13 silencing could induce ROS generation and cause DNA damage."
| PMC
USP13 affects DIABLO
1 |
1 |

No evidence text available
USP13 affects Chk1 phosphorylation
| 1
USP13 activates Chk1 phosphorylation. 1 / 1
| 1

eidos
"Since Chk1 phosphorylation is abrogated by USP13 deficiency , we tested whether USP13 is involved in cell cycle checkpoint activation following replication stress ."
USP13 affects CK2
| 1
CK2 binds USP13 and Thr122. 1 / 1
| 1

reach
"We further demonstrated that Casein kinase 2 (CK2) directly interacts with and phosphorylates USP13 at Thr122, which promotes the stability of USP13 protein."
USP13 affects CIN3
| 1
USP13 activates CIN3. 1 / 1
| 1

reach
"USP13 mRNA expression and the levels of USP13 protein both increased during progression through CIN1 to CIN3."
USP13 affects CD4
| 1
USP13 activates CD4. 1 / 1
| 1

eidos
"In addition , in B-cells and CD4 + T-cells , high amplifications and arm level gains of USP13 resulted in increased tumor infiltration ."
| PMC
USP13 affects CD3delta
| 1
USP13 increases the amount of CD3delta. 1 / 1
| 1

reach
"As a result, USP13 can significantly increase the protein level of CD3delta, whereas its C345A mutant loses its ability (XREF_FIG)."
USP13 affects BRCC36
| 1
| 1

sparser
"For example, the three deubiquitinating enzymes BRCC36, USP13, and USP39 interact with STAT1 and decrease the K63-, K48- and K6-linked polyubiquitin chains of STAT1 respectively (110, 115, 118)."
USP13 affects BRCA1-A
| 1
USP13 activates BRCA1-A. 1 / 1
| 1

reach
"In addition, reconstitution of WT USP13, but not the T196A mutant, fully rescued the foci formation of the BRCA1-A complex, DNA repair and cell cycle checkpoint in USP13 deficient cells and reversed hypersensitivity to cisplatin or olaparib induced by USP13 deficiency (XREF_FIG and XREF_SUPPLEMENTARY)."
USP13 affects BCL2L1
| 1
USP13 inhibits BCL2L1. 1 / 1
| 1

reach
"We further report that genetic or pharmacological inhibition of USP13 considerably reduces MCL1 protein abundance and significantly increases tumor cell sensitivity to BH3 mimetic inhibitors targeting BCL-2 and BCL-XL."
USP13 affects BCL2
| 1
USP13 activates BCL2. 1 / 1
| 1

reach
"We further report that genetic or pharmacological inhibition of USP13 considerably reduces MCL1 protein abundance and significantly increases tumor cell sensitivity to BH3 mimetic inhibitors targeting BCL-2 and BCL-XL."
USP13 affects BAG6 cleavage
| 1
USP13 inhibits BAG6 cleavage. 1 / 1
| 1

eidos
"These results suggest that USP13 depletion activates caspases and induces BAG6 cleavage ."
| 1
| 1

eidos
"In addition , in B-cells and CD4 + T-cells , high amplifications and arm level gains of USP13 resulted in increased tumor infiltration ."
| PMC
USP13 affects ATR
| 1
USP13 inhibits ATR. 1 / 1
| 1

reach
"Depletion of USP13 impedes ATR activation and hypersensitizes cells to replication stress inducing agents."
USP13 affects ATP
| 1
USP13 inhibits ATP. 1 / 1
| 1

reach
"In support of the involvement of USP13 in bioenergetics, USP13-KD inhibited ATP production in cells (XREF_FIG)."
USP13 affects ATM
| 1
USP13 activates ATM. 1 / 1
| 1

reach
"Unexpectedly, USP13 could not reverse the inhibition of ATM activity in shRAP80 transfected cells."
USP13 affects AKT inhibitors
| 1
USP13 activates AKT inhibitors. 1 / 1
| 1

reach
"The results suggest that inhibition of USP13 may enhance the effects of PI3K and AKT inhibitors on killing OVCA cells."
USP13 inhibitor affects USP13
| 1
USP13 inhibitor activates USP13. 1 / 1
| 1

reach
"USP13 inhibitor, Spautin-1, treatment also impaired HR in control but not USP13 deficient cells (XREF_SUPPLEMENTARY)."
USP11 affects USP13
| 1
| 1

sparser
"PTEN and DUBs were readily co-immunoprecipitated with each other; however, USP11, USP13, and OTUD3 were not associated with each other (Supplementary Fig.  xref )."
USP10 affects SKP2
| 1
SKP2 binds USP10 and USP13. 1 / 1
| 1

sparser
"The present study uncovers that USP10 is another critical DUB that deubiquitinates SKP2 and suppresses the ubiquitin-dependent proteasomal degradation of SKP2, which is compellingly supported by multiple lines of evidence: (1) our Co-IP experiments revealed compelling interaction of endogenous SKP2 with not only USP13 but also USP10 in KBM5-T315I and KBM5 cells (Fig.  xref ); (2) the interaction of overexpressed SKP2 with overexpressed USP13 or USP10 was also confirmed in HEK293T cells but the USP10 missing the N-terminal 205 amino acid residues failed to bind to SKP2 (Fig.  xref ); (3) overexpression of either USP13 or USP10 led to a higher SKP2 protein level and decreased p27 but overexpression of a DUB activity-disabled USP10 failed to do so (Fig.  xref ); (4) shRNA-mediated knockdown or pharmacological inhibition of USP10 with Spautin1 remarkable decreased SKP2 and phosphorylated Bcr-Abl (but not total Bcr-Abl) but increased p27 in multiple CML cell lines (Fig.  xref , Fig.  xref ); (5) CHX chase assays showed that shortened the halflife of SKP2 protein (Fig.  xref ); and (6) both pharmacological and genetic inhibition of USP10 could markedly increase K48-linked ubiquitinated SKP2 (Fig.  xref )."
USP10 affects MAP1LC3B
| 1
| 1

sparser
"We also used an in vitro assay to test the effect of treating HA-Ub-conjugated FLAG-LC3B with recombinant His6-tagged USP10 (His6-USP10) or His6-tagged USP13 (His6-USP13) (specificity control)."
USP-Y708F affects USP13
| 1
USP-Y708F activates USP13. 1 / 1
| 1

reach
"CHX chase assays indicated that overexpression of WT-USP13, particularly a phosphomimetic USP13-Y708E, but not USP-Y708F, significantly up-regulated the half-time of the c-Myc protein in HEK293 cells (XREF_FIG)."
UFD1 affects VCP
| 1
| 1

sparser
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
UBE2I affects USP13
| 1
UBE2I binds USP13 and MuRF. 1 / 1
| 1

sparser
"MURF family members interact with Ubc9 and ISOT-3, but only MURF-1 interacts with the transcriptional modulator GMEB-1."
Thr122 affects USP13
| 1
CK2 binds USP13 and Thr122. 1 / 1
| 1

reach
"We further demonstrated that Casein kinase 2 (CK2) directly interacts with and phosphorylates USP13 at Thr122, which promotes the stability of USP13 protein."
TWIST1 affects USP13
| 1
TWIST1 increases the amount of USP13. 1 / 1
| 1

reach
"In addition, we found that the protein levels of Twist1 dose-dependently increased with USP13 overexpression, while USP13 knockdown resulted in a decreased expression of endogenous Twist1."
THOC1 affects USP13
1 |
1 |

No evidence text available
TCAF1 affects USP13
1 |
1 |

No evidence text available
TBK1 affects USP13
| 1
| 1

sparser
"In support of this notion, we observed that (i) USP13 deficiency in mice or knockdown of USP13 in human cells potentiated HSV-1-triggered activation of IRF3 and NF-κB and subsequent induction of type I IFNs and proinflammatory cytokines, (ii) USP13 deficient cells restricted HSV-1 replication more profoundly than did the wild-type controls and USP13 deficient mice were more resistant to lethal HSV-1 infection compared to the wild-type littermates, (iii) USP13 but not USP13(AE) removed K27-linked ubiquitination of STING in cells or in vitro , and (iv) overexpression of USP13 specifically disrupted TBK1-STING association and knockdown or knockout of USP13 potentiated STING-TBK1 association after HSV-1 infection."
TBCB affects USP13
| 1
USP13 binds BAG6 and TBCB. 1 / 1
| 1

sparser
"We next tested whether USP13 could physically interact with Bag6 and/or its cofactors."
Spautin-1 inhibitor affects USP13
| 1
Spautin-1 inhibitor inhibits USP13. 1 / 1
| 1

reach
"What 's more, the Spautin-1 inhibitor could inhibit autophagy by reducing the activity of USP10 and USP13 XREF_BIBR."
Soot affects USP13
1 |
Soot increases the amount of USP13. 1 / 1
1 |

No evidence text available
STING1 affects TBK1, and USP13
| 1
| 1

sparser
"In support of this notion, we observed that (i) USP13 deficiency in mice or knockdown of USP13 in human cells potentiated HSV-1-triggered activation of IRF3 and NF-κB and subsequent induction of type I IFNs and proinflammatory cytokines, (ii) USP13 deficient cells restricted HSV-1 replication more profoundly than did the wild-type controls and USP13 deficient mice were more resistant to lethal HSV-1 infection compared to the wild-type littermates, (iii) USP13 but not USP13(AE) removed K27-linked ubiquitination of STING in cells or in vitro , and (iv) overexpression of USP13 specifically disrupted TBK1-STING association and knockdown or knockout of USP13 potentiated STING-TBK1 association after HSV-1 infection."
STAT1 affects USP39
| 1
| 1

sparser
"For example, the three deubiquitinating enzymes BRCC36, USP13, and USP39 interact with STAT1 and decrease the K63-, K48- and K6-linked polyubiquitin chains of STAT1 respectively (110, 115, 118)."
SPRR2C affects USP13
1 |
1 |

No evidence text available
SNAI1 affects USP13
| 1
| 1

sparser
"USP13 interacted with Snail to deubiquitinate and stabilize Snail in GC cells."
SKP2 affects USP10, and USP13
| 1
SKP2 binds USP10 and USP13. 1 / 1
| 1

sparser
"The present study uncovers that USP10 is another critical DUB that deubiquitinates SKP2 and suppresses the ubiquitin-dependent proteasomal degradation of SKP2, which is compellingly supported by multiple lines of evidence: (1) our Co-IP experiments revealed compelling interaction of endogenous SKP2 with not only USP13 but also USP10 in KBM5-T315I and KBM5 cells (Fig.  xref ); (2) the interaction of overexpressed SKP2 with overexpressed USP13 or USP10 was also confirmed in HEK293T cells but the USP10 missing the N-terminal 205 amino acid residues failed to bind to SKP2 (Fig.  xref ); (3) overexpression of either USP13 or USP10 led to a higher SKP2 protein level and decreased p27 but overexpression of a DUB activity-disabled USP10 failed to do so (Fig.  xref ); (4) shRNA-mediated knockdown or pharmacological inhibition of USP10 with Spautin1 remarkable decreased SKP2 and phosphorylated Bcr-Abl (but not total Bcr-Abl) but increased p27 in multiple CML cell lines (Fig.  xref , Fig.  xref ); (5) CHX chase assays showed that shortened the halflife of SKP2 protein (Fig.  xref ); and (6) both pharmacological and genetic inhibition of USP10 could markedly increase K48-linked ubiquitinated SKP2 (Fig.  xref )."
SKP2 affects UCHL5, USP10, USP13, USP14, and USP7
| 1
| 1

sparser
"We found that SKP2 interacted with USP10, USP13, UCHL5, USP14, and USP7 in KBM5-T315I cells (Fig.  xref )."

reach
"26 The role of autophagy in cerebral ischaemia‐reperfusion needs to be further investigated.Spautin‐1 was further used to investigate the mechanism of autophagy activation on cerebral ischaemia‐reperfusion injury, and Spautin‐1 is as an inhibitor of USP13."

eidos
"MITF-M protein level was significantly reduced when the USP13 expression was suppressed by siRNA ."
RI affects USP13
| 1
RI inhibits USP13. 1 / 1
| 1

reach
"FNT reduced the FcεRIγ chain expression, via increased proteasome-mediated degradation, and induced FcεRIγ ubiquitination by inhibiting USP5 and/or USP13."
REPIN1 affects USP13
1 |
REPIN1 decreases the amount of USP13. 1 / 1
1 |

No evidence text available
RAD23A affects USP13
1 |
1 |

No evidence text available
QHO60603 affects USP13
1 |
USP13 binds QHO60603. 1 / 1
1 |

No evidence text available
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sparser
"Like NLRP3, the NLRP3 NACHT domain and LRR domain interacted with USP13, but the NLRP3 PYRIN domain failed to interact with USP13 (Fig.  xref h)."

eidos
"Inhibition of c-Jun N-terminal kinase ( JNK ) attenuates USP13 degradation , indicating that a JNK-dependent new protein synthesis is necessary for USP13 degradation ."
Particulate Matter decreases the amount of USP13. 1 / 1
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No evidence text available
PTEN affects OTUD3
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USP13 binds OTUD3 and PTEN. 1 / 1
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sparser
"USP7, a highly expressed DUB in prostate cancer and progressive multifocal leukoencephalopathy (PML), plays a direct role in PTEN deubiquitination and regulates its localization rather than protein stability. xref In addition, USP13 and OTUD3 can interact with PTEN and remove its polyubiquitin chain."
PMS1 affects USP13
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No evidence text available
PITX2 affects USP13
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PITX2 decreases the amount of USP13. 1 / 1
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No evidence text available
PAX5 affects USP13
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PAX5 decreases the amount of USP13. 1 / 1
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No evidence text available
OTUD3 affects USP11, and USP13
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sparser
"PTEN and DUBs were readily co-immunoprecipitated with each other; however, USP11, USP13, and OTUD3 were not associated with each other (Supplementary Fig.  xref )."
OTUD3 affects PTEN, and USP13
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USP13 binds OTUD3 and PTEN. 1 / 1
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sparser
"USP7, a highly expressed DUB in prostate cancer and progressive multifocal leukoencephalopathy (PML), plays a direct role in PTEN deubiquitination and regulates its localization rather than protein stability. xref In addition, USP13 and OTUD3 can interact with PTEN and remove its polyubiquitin chain."
Neoplasms affects USP13
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"The therapeutic potential of miR‐367 was further confirmed in vivo, in an orthotopic xenograft model of aggressive embryonal CNS tumors initiated by OCT4A‐overexpressing cells (Daoy, USP13‐MED, or USP7‐ATRT) in Balb/C nude mice."
NRGN affects USP13
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NRGN inhibits USP13. 1 / 1
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eidos
"LPS treatment starting from 10 ng / ml decreased USP13 levels after 24 h ( Fig. 2A-D ) ."
NPLOC4 affects VCP
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sparser
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
NF-kB p65 affects USP13
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Modified NF-kB p65 decreases the amount of USP13. 1 / 1
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"Results suggested that the overexpression of NF-kB p65 significantly decreased the expression levels of USP13 and PTEN, and re-introduction of USP13 rescued PTEN expression."
NEDD8 affects USP13
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No evidence text available
NEDD4 affects USP13
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NEDD4 activates USP13. 1 / 1
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"A recent study identified a role of USP13 in VPS34 deubiquitination and stabilization and NEDD4-1 autoubiquitination promotes the recruitment of USP13 to VPS34 35."
MuRF affects USP13
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UBE2I binds USP13 and MuRF. 1 / 1
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sparser
"MURF family members interact with Ubc9 and ISOT-3, but only MURF-1 interacts with the transcriptional modulator GMEB-1."
MOV10 affects USP13
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No evidence text available
MLN7243 affects USP13
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MLN7243 desumoylates USP13. 1 / 1
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No evidence text available
MITF affects USP13
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MITF activates USP13. 1 / 1
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"More importantly, USP13 deficiency resulted in impaired melanoma growth both in vitro and in vivo, and reintroduction of MITF reversed the inhibitory effect of USP13, demonstrating that MITF contributed to melanoma progression under the ubiquitination modification of USP13 95."
METTL3 affects USP13
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USP13 binds METTL3 and K48. 1 / 1
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"USP13 interacts with, deubiquitinates, and therefore stabilizes METTL3 at K488 by removing K48-linked ubiquitin chains."
MCL1 affects FlaG
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USP13 binds MCL1 and FlaG. 1 / 1
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sparser
"Immunoprecipitation with anti-FLAG antibody demonstrated that FLAG-USP13 interacted with over-expressed Mcl-1 (Fig. xref )."
MAP1LC3B affects USP13
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"We also used an in vitro assay to test the effect of treating HA-Ub-conjugated FLAG-LC3B with recombinant His6-tagged USP10 (His6-USP10) or His6-tagged USP13 (His6-USP13) (specificity control)."

reach
"Lipopolysaccharide reduces USP13 stability through c-Jun N-terminal kinase activation in Kupffer cells."
KCTD3 affects USP13
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No evidence text available
K48 affects USP13
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USP13 binds METTL3 and K48. 1 / 1
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"USP13 interacts with, deubiquitinates, and therefore stabilizes METTL3 at K488 by removing K48-linked ubiquitin chains."
JNK inhibitor affects USP13
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JNK inhibitor activates USP13. 1 / 1
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"Furthermore, among the inhibitors of matrix metalloproteinases (MMPs), ROCK, p38 MAPK, and JNK, only JNK inhibitor II attenuated USP13 degradation (Fig. 7D)."
JNK depletion affects USP13
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JNK depletion activates USP13. 1 / 1
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eidos
"In this study , we demonstrated that inhibition of JNK , or depletion of c-Jun , resulted in a substantial loss of USP13 expression and this was regulated at the transcriptional level ( Fig. 2B-E ) ."
ITCH affects USP13
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No evidence text available
IRF3 affects USP13
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"Although USP13 interacted with cGAS and IRF3 in an overexpression system, USP13 did not affect the ubiquitination of cGAS or IRF3 when coexpressed in HEK293 cells (XREF_SUPPLEMENTARY)."
Hypoxia affects USP13
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"( D ) Hypoxia increased USP13 , TLR4 , MyD88 , and P-NF-kappaB p65 proteins , which was subsequently reduced by USP13 knockdown ."
HPV oncogenes affects USP13
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HPV oncogenes activates USP13. 1 / 1
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"To investigate if the HPV oncogenes could directly induce USP13 expression , we first transiently expressed HR-HPV E6 and E7 , alone or in combination , in HPV - C33A cells ."
Gasoline affects USP13
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Gasoline decreases the amount of USP13. 1 / 1
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No evidence text available
GST affects USP5
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GST binds USP13, USP5, and VCP. 1 / 1
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sparser
"By contrast, neither GST-USP13 nor GST-USP5 bound p97 ( xref )."
GST affects USP13, and VCP
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GST binds USP13 and VCP. 1 / 1
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sparser
"We expressed these proteins in cells individually and examined the binding of endogenous p97 to GST-USP13."
GST affects USP13, USP5, and VCP
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GST binds USP13, USP5, and VCP. 1 / 1
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sparser
"By contrast, neither GST-USP13 nor GST-USP5 bound p97 ( xref )."
GST affects BAG6
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GST binds USP13 and BAG6. 1 / 1
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"Indeed, GST pull-down experiments using purified proteins showed that GST-USP13 bound Bag6, and the N-terminal ubiquitin-like (UBL) domain in Bag6 was both necessary and sufficient for interaction with USP13 ( xref )."
GET4 affects USP13
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No evidence text available
GEMIN4 affects USP13
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"In addition, USP13 interacts with the P97 and valosin containing protein complex, a key chaperone in ER associated degradation, which increases the protein level of CD3delta, an ER associated degradation substrate."
FlaG affects USP10
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"We also used an in vitro assay to test the effect of treating HA-Ub-conjugated FLAG-LC3B with recombinant His6-tagged USP10 (His6-USP10) or His6-tagged USP13 (His6-USP13) (specificity control)."
FlaG affects MCL1, and USP13
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USP13 binds MCL1 and FlaG. 1 / 1
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"Immunoprecipitation with anti-FLAG antibody demonstrated that FLAG-USP13 interacted with over-expressed Mcl-1 (Fig. xref )."
FlaG affects MAP1LC3B, USP10, and USP13
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sparser
"We also used an in vitro assay to test the effect of treating HA-Ub-conjugated FLAG-LC3B with recombinant His6-tagged USP10 (His6-USP10) or His6-tagged USP13 (His6-USP13) (specificity control)."
FAF2 affects VCP
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sparser
"USP13 interacts with p97, UFD1, NPL4, and UBXD8, and upon USP13 deletion, cells are more susceptible to ER stress."
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E3_Ub_ligase ubiquitinates USP13. 1 / 1
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"SKP2 is itself ubiquitylated by the E3 ligases APC/C CDH1 and SCF FBXW2, and deubiquitylated by deubiquitylases (DUBs) USP10 and USP13."
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sparser
"Although the interactions between MYC and some E3 ligases and deubiquitinases, such as CHIP, FBXL14, USP13 and USP22, are not identified yet in prostate cancer, the similar molecular mechanism enable them as probable targets for MYC degradation in prostate cancer."
DIABLO affects USP13
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No evidence text available

reach
"The therapeutic potential of miR‐367 was further confirmed in vivo, in an orthotopic xenograft model of aggressive embryonal CNS tumors initiated by OCT4A‐overexpressing cells (Daoy, USP13‐MED, or USP7‐ATRT) in Balb/C nude mice."
CK2 affects USP13
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CK2 binds USP13 and Thr122. 1 / 1
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"We further demonstrated that Casein kinase 2 (CK2) directly interacts with and phosphorylates USP13 at Thr122, which promotes the stability of USP13 protein."
CDCA8 affects USP13
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CDCA8 increases the amount of USP13. 1 / 1
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"Next-generation sequencing and laboratory validation showed that CDCA8 silencing inhibited the expression of TPM3, NECAP2, and USP13."
CDC5L affects USP13
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CDC5L decreases the amount of USP13. 1 / 1
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No evidence text available
CBLB affects USP13
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CBLB activates USP13. 1 / 1
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"c-Cbl enhanced the interaction between EGFR-DeltaE740-A746 and USP13, while Cbl-b increased the binding of both c-Cbl and USP13 to mutant EGFR."
BRCC36 affects USP39
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sparser
"For example, the three deubiquitinating enzymes BRCC36, USP13, and USP39 interact with STAT1 and decrease the K63-, K48- and K6-linked polyubiquitin chains of STAT1 respectively (110, 115, 118)."
BLM affects USP13
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BLM increases the amount of USP13. 1 / 1
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"In this study, we observed that both BLM and TGF-β1 increased USP13 protein levels in isolated primary mouse lung fibroblast cells, suggesting a role of USP13 in lung fibroblasts in the pathogenesis of pulmonary fibrosis."
BAG6 affects ubiquitin-like
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USP13 binds BAG6 and ubiquitin-like. 1 / 1
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"Indeed, GST pull-down experiments using purified proteins showed that GST-USP13 bound Bag6, and the N-terminal ubiquitin like (UBL) domain in Bag6 was both necessary and sufficient for interaction with USP13 (XREF_FIG)."
BAG6 affects TBCB, and USP13
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USP13 binds BAG6 and TBCB. 1 / 1
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"We next tested whether USP13 could physically interact with Bag6 and/or its cofactors."
BAG6 affects GST, and USP13
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GST binds USP13 and BAG6. 1 / 1
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sparser
"Indeed, GST pull-down experiments using purified proteins showed that GST-USP13 bound Bag6, and the N-terminal ubiquitin-like (UBL) domain in Bag6 was both necessary and sufficient for interaction with USP13 ( xref )."
AMFR affects VCP
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USP13 binds VCP and AMFR. 1 / 1
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sparser
"These results demonstrated a specific and direct interaction between USP13 and gp78 that facilitates USP13-p97 association."
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No evidence text available
1-N-methyl-5-thiotetrazole increases the amount of USP13. 1 / 1
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No evidence text available
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No evidence text available