IndraLab

Statements


USP10 affects TP53
7 1 | 5 134 35
USP10 deubiquitinates TP53.
1 | 59
USP10 deubiquitinates TP53. 10 / 58
1 | 57

reach
"Because USP10 is a p53 deubiquitinating enzyme, p53 ubiquitination was decreased by transfection of USP10 (compare lanes 3 and 4), whereas deubiquitination of p53 by USP10 was inhibited by co-transfection of USP10 and G3BP1 (compare lanes 4 and 5)."

reach
"Under stress condition, ATM phosphorylates USP10, which then deubiquitinates and targets cytoplasmic p53 back to the nucleus."

reach
"These results demonstrate that USP10 deubiquitinates p53 both in vitro and in vivo."

reach
"USP10 deubiquitinates both wild-type p53 and mutp53."

reach
"Moreover, they also found that p53, which can be deubiquitinated by USP10, negatively regulates the expression of miR-138 through binding to the miRNA’s promoter region [31]."

reach
"USP10 deubiquitinates p53 and reverses MDM2 mediated nuclear transport and degradation of p53."

reach
"Here we report that USP10, a cytoplasmic ubiquitin specific protease, deubiquitinates p53, reversing Mdm2 induced p53 nuclear export and degradation."

reach
"Resveratrol (RSVL) directly binds to G3BP1 and prevents the interaction of G3BP1/USP10, which enhances the USP10-mediated de-ubiquitination of p53, thereby increasing p53 expression (135)."

reach
"G3BP1 also inhibited USP10 mediated deubiquitination of endogenous p53 in HCT116 cells (XREF_FIG)."

reach
"Once in the cytoplasm, p53 can be deubiquitinated by the deubiquitinase USP10, resulting in its translocation back to the nucleus (Yuan et al., 2010) [pathway (3) in Figure 2]."
USP10 leads to the deubiquitination of mutated TP53. 2 / 2
| 2

reach
"However, it is unclear whether USP10 antagonizes ubiquitination of mutp53 by other ubiquitin ligases, USP10 alters subcellular localization of mutp53, and USP10 plays a role in deubiquitination of different TP53 mutants."

reach
"USP10 deubiquitinates and stabilizes both wt and mutant p53."
USP10 binds TP53.
7 | 17 32
7 | 17 27

sparser
"In unstressed cells, it was shown that USP10 binds, deubiquitinates and stabilizes p53 in the cytoplasm, antagonizing Mdm2-mediated p53 nuclear export and degradation."

No evidence text available

sparser
"USP10 is also associated with regulation of p53 localization and stability, however, unlike USP2 and USP7, it does not interact with MDM2 [ xref ]."

No evidence text available

reach
"The inhibition of the proline hydroxylase PHD3 could significantly reduce the binding of USP10 to p53 [35]."

sparser
"G3BP1 disrupts the interaction of USP10 with p53."

reach
"IGF2BP3 (insulin-like growth factor 2 mRNA binding protein 3) inhibits p53 expression through disrupting the interaction between USP10 and p53 and promotes lung cancer progression [37]."

sparser
"These results suggest that G3BP1 interrupts the interaction of USP10 with p53 by its own interaction with USP10."

sparser
"Similar to what we observed with USP7, inhibition of hydroxylase activity diminished the interaction between p53 and endogenous USP10."

reach
"In other words, in the presence of p53, USP10 largely binds p53 and stabilizes p53 under the stress conditions."
TP53 binds USP10 and USP7. 3 / 3
| 3

sparser
"For example, USP7 and USP10 bind to and deubiquitylate their substrate p53 to mediate its role for suppression of cell propagation upon cellular stresses by counteracting its degradation xref – xref ."

sparser
"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced under DMOG treatment ( xref A)."

sparser
"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced un[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
TP53 binds USP10 and G3BP1. 2 / 2
| 2

sparser
"Disruption of the interaction of p53 with USP10 by G3BP1 interference leads to suppression of p53 deubiquitination."

sparser
"Disruption of the interaction of p53 with USP10 by G3BP1 interference leads to the suppression of p53 deubiquitination."
USP10 activates TP53.
| 4 26 1
USP10 activates TP53. 10 / 33
| 4 23 1

reach
"Taken together, our findings disclose a novel mechanism that hypoxia-inducible circWSB1 could interact with USP10 to attenuate USP10 mediated p53 stabilization and promote the progression of BC, providing an alternative prognostic biomarker and therapeutic target for BC."

reach
"Upon DNA damage, USP10 deubiquitylates and activates p53."

reach
"Upon cellular stresses, such as DNA damage or oncogene activation, the TP53 degradation process is attenuated by USP7 and USP10, two deubiquitinating enzymes."

reach
"For example, USP7 regulates the stability of both p53 and Mdm2 and maintains p53 ubiquitination levels; 120 USP2 mediates the stability of Mdm2; 121 USP10 modulates p53 localization and stability; 122 OTUB1 abrogates p53 ubiquitination and activates p53."

reach
"USP10 modulates anterograde and retrograde vesicular trafficking, the localization and stability of p53 and binds Dengue virus RNA."

reach
"Previous report showed that USP10 positively regulate TP53."

reach
"In the case of DNA damage, USP10 is stabilized and translocates to the nucleus to activate and stabilize p53."

reach
"Wildtype TP53 is usually rescued from proteasomal degradation by USP10."

reach
"It will be interesting to discover whether overexpression of USP7 could also rescue the diminished DNA damage induced p53 response caused by USP10 depletion."

eidos
"After DNA damage , USP10 is stabilised , and a fraction of USP10 translocates to the nucleus to activate p53 ."
USP10 activates mutated TP53. 3 / 3
| 3

reach
"Paradoxically, USP10 could promote cancer cell proliferation in mutant p53 background."

reach
"Notably, as USP10 can positively regulate both wild-type and mutant TP53, therefore, miR-138 could also play a dual role as an either tumor suppressor or an onco-miR in different cell context."

reach
"Furthermore, expression of USP10 increased mutant p53 stability (XREF_SUPPLEMENTARY), while downregulation of USP10 decreased mutant p53 stability (XREF_SUPPLEMENTARY), suggesting that USP10 regulates mutant p53 stability."
USP10 inhibits TP53.
| 1 15 1
USP10 inhibits TP53. 10 / 25
| 1 15 1

reach
"Expression of USP10 significantly suppressed c-Myc transcriptional activity in p53 wildtype and p53 -/- HCT116 cells, but the levels of USP10 mediated suppression in c-Myc target gene transcription in p53-null cells were partially reversed."

reach
"In addition, USP10 suppresses the tumour cell growth in cells with wild-type p53, and USP10 expression is down-regulated in a high percentage of clear cell carcinomas known to have few p53 mutations [108]."

reach
"It also binds USP10, a key p53 regulator, reducing p53 stability and its anti-cancer functions."

reach
"Indeed, USP10 depletion attenuates DNA damage induced stabilization of p53, an effect that was rescued by overexpression of wild-type USP10 but not by mutant USP10 lacking ATM phosphorylation sites."

reach
"Association of USP10 with G3BP2 Inhibits p53 Signaling and Contributes to Poor Outcome in Prostate Cancer."

reach
"The research by Yuan et al. demonstrated that USP10 inhibits cancer cell proliferation in wild-type p53 cells, but promotes tumorigenesis in a mutant p53 background (Yuan et al., 2010)."

reach
"For example, USP10 regulates Notch signaling pathway in the endothelium [23]; USP10 protects against cerebral ischemia injury by attenuating inflammation and apoptosis through inhibiting the TAK1 signaling pathway [8]; Interaction of USP10 and G3BP2 blocks p53 signaling and results in poor prognosis in prostate cancer [24]; Wu-5, which is an inhibitor of USP10, enhances crenolanib-induced FLT3-ITD-positive AML cell death by suppressing FLT3 and AMPK signaling pathways [25]."

reach
"Usp10 overexpression abolished the estrogen mediated regulation of p53 and the downstream transcriptional gene p21."

reach
"The association of USP10 with G3BP2 suppresses p53 signalling which correlates to a poor prognosis in prostate cancer, highlighting an oncogenic role of USP10 in prostate cancer via G3BP2 [XREF_BIBR]."

eidos
"USP10 can interact with G3BP2 to block p53 signaling and subsequently contributes to a poor prostate cancer prognosis [ 50 ] ."
USP10 increases the amount of TP53.
| 8
USP10 increases the amount of TP53. 5 / 6
| 5

reach
"Reconstitution of USP10 in CAKI-1 and CAKI-2 (WT p53 cell lines) restored p53 expression and increased p21 and BAX expression (XREF_SUPPLEMENTARY)."

reach
"In accordance with published work, we detected that knockdown of USP10 is sufficient to reduce p53 protein levels."

reach
"These results suggest that USP10 can upregulate p53 levels, most likely by deubiquitinating and consequently stabilizing p53."

reach
"In accordance with published work, we detected that knockdown of USP10 is sufficient to reduce p53 protein levels (Yuan et al., 2010)."

reach
"Both, the DUB HAUSP (herpesvirus associated ubiquitin specific protease) and USP10 (ubiquitin specific protease 10), targeting poly-ubiquitinated p53, have been shown to restore p53 levels even when Hdm2 is overexpressed."
Modified USP10 increases the amount of TP53. 3 / 3
| 3

reach
"Overexpression of USP10 significantly increased the levels of endogenous p53, with no effect on p53 mRNA levels (XREF_FIG)."

reach
"Consistent with this study, we show that increased USP10 expression in mutant p53 background increases p53 levels and promotes cancer cell proliferation, while downregulation of USP10 inhibits cancer cell growth."

reach
"Furthermore, overexpression of USP10 in 786-O cells increases levels of mutp53, leading to enhanced colony formation and proliferation, which is nullified by depletion of mutp53."
USP10 ubiquitinates TP53.
| 5 1
USP10 leads to the ubiquitination of TP53. 5 / 6
| 4 1

sparser
"Depletion of USP10 enhanced p53 ubiquitination (compare lanes 1 and 3), whereas enhancement of p53 ubiquitination by G3BP1 was not observed in USP10 depleted cells (compare lanes 3 and 4)."

reach
"In the 786-O RCC cell line, USP10 overexpression inhibits MDM2 mediated mutp53 ubiquitination leading to stabilization of mutp53, while USP10 downregulation increases mutp53 ubiquitination to decrease its stability."

reach
"Furthermore, downregulation of USP10 increased the ubiquitination of p53 in the cytoplasm, but not in the nucleus (XREF_FIG)."

reach
"Depletion of USP10 enhanced p53 ubiquitination (compare lanes 1 and 3), whereas enhancement of p53 ubiquitination by G3BP1 was not observed in USP10 depleted cells (compare lanes 3 and 4)."

reach
"Conversely, downregulation of USP10 increased p53 ubiquitination (XREF_FIG)."
Modified USP10 leads to the ubiquitination of TP53. 1 / 1
| 1

reach
"As shown in XREF_FIG, Mdm2 induced the ubiquitination of p53; however, p53 ubiquitination was significantly diminished by coexpression of USP10 or HAUSP."
USP10 decreases the amount of TP53.
| 4
USP10 decreases the amount of TP53. 4 / 4
| 4

reach
"Interestingly, downregulation of USP10 significantly decreased p53 stabilization and the expression of p53 target genes p21 and Bax after DNA damage (XREF_FIG)."

reach
"On the other side, the ubiquitinated TP53 could be reversed and stabilized by USP7 and USP10, to keep the amount of TP53 in check."

reach
"Thus, depletion of USP10 would cause a decrease in p53 levels and lead to cisplatin resistance."

reach
"To confirm the role of USP10 in regulating p53 levels, we depleted USP10 using USP10 specific shRNA, and found that downregulation of USP10 decreased p53 protein levels with no effect on p53 mRNA levels (XREF_FIG)."
G3BP1 affects USP10
11 1 | 78 74
G3BP1 binds USP10.
11 1 | 63 70
11 1 | 63 46

reach
"Therefore, although Caprin1 and USP10 both bind G3BP, they have antagonistic effects on SGs."

sparser
"Thus, studies were conducted to determine if Cif inhibits USP10 activity by stabilizing the interaction between USP10 and G3BP1, and by reducing the interaction between USP10 and CFTR."

reach
"Studies using USP10 mutants, including USP10/77 -792 and USP10/95-792, indicate that USP10 's interaction with G3BP1 is essential for inhibition of ROS dependent apoptosis and for USP10 incorporation into stress granules."

reach
"Resveratrol promotes the p53-mediated apoptosis of cancer cells by acting on the USP10-binding protein G3BP1 [36]."

reach
"To provide additional support for the immunoprecipitation studies demonstrating that Cif increases the interaction between G3BP1 and USP10 in the early endosomal compartment, we performed bimolecular fluorescence complementation (BiFC) studies."

sparser
"Cif stabilizes USP10 and G3BP1 interaction to inhibit USP10 activity."

sparser
"Interestingly, recent work has shown that the compound resveratrol can inhibit the interaction of G3BP with USP10 by binding to the NTF2-like domain of G3BP, thereby stimulating USP10 DUB activity and stabilization of p53 [ xref ]."

sparser
"TAT-RasGAP 317–326 did not modulate binding of G3BP1 to USP10, stress granule formation or c-myc mRNA levels."

sparser
"Thus, USP10 interaction with G3BP1 and the incorporation of USP10 into stress granules are dispensable for USP10 inhibition of cytokine deprivation-induced apoptosis in FL HSPCs."

reach
"USP10 and Caprin1 antagonistically bind G3BP to regulate SG formation."
| 7

sparser
"However, a recent study suggests that G3BP proteins interact with Caprin1 and USP10 but that they are mutually exclusive allowing the binding of these proteins to serve as a switch for SG formation depending on the binding partner interacting with G3BP1."

sparser
"However, it is important to note that the interactions between G3BP1 and USP10 or Caprin1 do not fully dictate SG disassembly or assembly, as two different laboratories recently demonstrated that the unstressed G3BP1 interactome significantly overlaps the interactome of G3BP1 during stress [ xref , xref ]."

sparser
"USP10 and Caprin1 antagonistically bind G3BP to regulate SG formation."

sparser
"Our results indicated that N expression abrogates G3BP1 interaction with the SG proteins CAPRIN1 and USP10 ( Figure 2C ), reinforcing the idea that N inhibits the formation of SGs in host cells by sequestering G3BPs away from their interacting SG-nucleating proteins."

sparser
"Rescue experiments using G3BP1 mutants show that phosphomimetic G3BP1-S149E fails to rescue SG formation, whereas G3BP1-F33W, a mutant unable to bind G3BP partner proteins Caprin1 or USP10, rescues SG formation."

sparser
"Therefore, although Caprin1 and USP10 both bind G3BP ( xref ; xref ), they have antagonistic effects on SGs."

sparser
"Interaction of Dengue RNA with stress granule proteins G3BP1, USP10 and caprin1 occurs at an adjacent unstructured region of the 3′ UTR ( xref )."
| 6

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [ xref ], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [ xref ]."

sparser
"However, a role for the G3BP1 and G3BP2 interactions with CAPRIN1 and/or USP10 under chronic nutrient starvation has not yet been investigated."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al.2017); SeV Trailer RNA captures TIAR from SG (Iseni et al.2002); West Nile Virus (WNV) and Dengue virus (DENV) 3′-end viral genome captures TIA-1/TIAR (Li et al.2002; Emara and Brinton 2007; Xia et al.2015); DENV 3′-UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al.2011; Reineke et al.2015); JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al.2011)."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al. 2017) ; SeV Trailer RNA captures TIAR from SG (Iseni et al. 2002) ; West Nile Virus (WNV) and Dengue virus (DENV) 3 0 -end viral genome captures TIA-1/TIAR (Li et al. 2002; Emara and Brinton 2007; Xia et al. 2015) ; DENV 3 0 -UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al. 2011; Reineke et al. 2015) ; JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al. 2011) ."

sparser
"Additional experiments demonstrate that, in contrast to DDX6, the SG proteins G3BP1, G3BP2, Caprin1 and USP10 bind to the variable region (VR) in the 3' UTR."

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [171], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [172]."
| 4

sparser
"Validation of the mass spectrometry data confirmed the interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."

sparser
"NCAP showed specific interaction with the SG proteins G3BP1, G3BP2, YTHDF3, USP10 and PKR, and translocated to SGs following oxidative stress and heat shock."

sparser
"NCAP interacts with SG proteins. (A) Interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."

sparser
"Fig. 4: NCAP interacts with SG proteins. (A) Interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."
| 3

sparser
"Since the binding of nsP3 and USP10 to G3BP inhibits SGs, we hypothesized that ICP8 binding might do the same."

sparser
"It was recently identified that a common canonical sequence motif, FGDF, mediates binding of the nsP3 and USP10 proteins to G3BP ."

sparser
"The model might also explain the elimination of binding of both nsP3 and USP10 to G3BP upon mutation of the glycine residue to alanine, which would restrict flexibility and thus hinder adequate positioning of the phenylalanine side-chains in the FGDF motifs."
| 2

sparser
"G3BP1 interacts with p62 and USP10 to inhibit protein aggregation."

sparser
"G3BP1 interacted with p62 and USP10 and inhibited p62/USP10-induced protein aggregation."
TP53 binds USP10 and G3BP1. 2 / 2
| 2

sparser
"Disruption of the interaction of p53 with USP10 by G3BP1 interference leads to suppression of p53 deubiquitination."

sparser
"Disruption of the interaction of p53 with USP10 by G3BP1 interference leads to the suppression of p53 deubiquitination."
G3BP1 inhibits USP10.
| 15 4
| 12 4

reach
"It should be remarked that G3BP1 is also a perturbator for the PD-VPs USP10 [91], GiGYF2 [99], EIF4G1 [100], and TIAL1 [101]."

reach
"G3BP1 disrupts the interaction of USP10 with p53."

sparser
"Anisonov et al. [91] also found that USP10 is inhibited by G3BP1 in a mechanism similar to that for NUP62, which indicates an analogous cascading route from SARS-CoV-2 to PD for this protein."

reach
"Surprisingly, the human homolog of Bre5, G3BP1, inhibits the activity of USP10, at least in vitro, indicating that cofactors can either restrict or enhance protease activity."

reach
"Notably, siRNA mediated knockdown of G3BP1 significantly increased the abundance of CFTR, most likely because endogenous G3BP1 inhibits USP10 (XREF_FIG)."

reach
"It appears therefore that the FGDF mediated G3BP and USP10 complex is mutually inhibitory, with G3BP inhibiting the DUB activity of USP10 and USP10 inhibiting the SG nucleating function of G3BP."

sparser
"In airway epithelial cells treated with the CFTR inhibitory factor (Cif), the Ras GTPase-activating protein-binding protein 1 (G3BP1) inhibits the ubiquitin-specific protease USP10."

reach
"discovered the interaction of G3BP1 with ubiquitin specific protease 10 (USP10), and they concluded that G3BP1 might restrict the deubiquitinating activity of USP10."

reach
"Taken together, these results suggested that G3BP1 inhibits p62/USP10-induced protein aggregation, whereas G3BP2 attenuates such a G3BP1 inhibition by competing G3BP1-interaction with p62 and/or USP10 (Fig. 8c)."

sparser
"Anisonov et al. [ xref ] also found that USP10 is inhibited by G3BP1 in a mechanism similar to that for NUP62, which indicates an analogous cascading route from SARS-CoV-2 to PD for this protein."
G3BP1 bound to USP10 inhibits USP10. 3 / 3
| 3

reach
"G3BP1 binds to USP10 and therefore disrupts the interaction of USP10 with p53, leading to suppression of p53 deubiquitination."

reach
"Second, in U2OS bladder cancer cells, the interaction between G3BP1 and USP10 inactivates the deubiquitinating enzyme activity of USP10 XREF_BIBR."

reach
"G3BP1 binds to USP10 and inhibits the deubiquitination activity of USP10."
USP10 affects G3BP1
11 1 | 67 70
USP10 binds G3BP1.
11 1 | 63 70
11 1 | 63 46

reach
"Therefore, although Caprin1 and USP10 both bind G3BP, they have antagonistic effects on SGs."

sparser
"Thus, studies were conducted to determine if Cif inhibits USP10 activity by stabilizing the interaction between USP10 and G3BP1, and by reducing the interaction between USP10 and CFTR."

reach
"Studies using USP10 mutants, including USP10/77 -792 and USP10/95-792, indicate that USP10 's interaction with G3BP1 is essential for inhibition of ROS dependent apoptosis and for USP10 incorporation into stress granules."

reach
"Resveratrol promotes the p53-mediated apoptosis of cancer cells by acting on the USP10-binding protein G3BP1 [36]."

reach
"To provide additional support for the immunoprecipitation studies demonstrating that Cif increases the interaction between G3BP1 and USP10 in the early endosomal compartment, we performed bimolecular fluorescence complementation (BiFC) studies."

sparser
"Cif stabilizes USP10 and G3BP1 interaction to inhibit USP10 activity."

sparser
"Interestingly, recent work has shown that the compound resveratrol can inhibit the interaction of G3BP with USP10 by binding to the NTF2-like domain of G3BP, thereby stimulating USP10 DUB activity and stabilization of p53 [ xref ]."

sparser
"TAT-RasGAP 317–326 did not modulate binding of G3BP1 to USP10, stress granule formation or c-myc mRNA levels."

sparser
"Thus, USP10 interaction with G3BP1 and the incorporation of USP10 into stress granules are dispensable for USP10 inhibition of cytokine deprivation-induced apoptosis in FL HSPCs."

reach
"USP10 and Caprin1 antagonistically bind G3BP to regulate SG formation."
| 7

sparser
"However, a recent study suggests that G3BP proteins interact with Caprin1 and USP10 but that they are mutually exclusive allowing the binding of these proteins to serve as a switch for SG formation depending on the binding partner interacting with G3BP1."

sparser
"However, it is important to note that the interactions between G3BP1 and USP10 or Caprin1 do not fully dictate SG disassembly or assembly, as two different laboratories recently demonstrated that the unstressed G3BP1 interactome significantly overlaps the interactome of G3BP1 during stress [ xref , xref ]."

sparser
"USP10 and Caprin1 antagonistically bind G3BP to regulate SG formation."

sparser
"Our results indicated that N expression abrogates G3BP1 interaction with the SG proteins CAPRIN1 and USP10 ( Figure 2C ), reinforcing the idea that N inhibits the formation of SGs in host cells by sequestering G3BPs away from their interacting SG-nucleating proteins."

sparser
"Rescue experiments using G3BP1 mutants show that phosphomimetic G3BP1-S149E fails to rescue SG formation, whereas G3BP1-F33W, a mutant unable to bind G3BP partner proteins Caprin1 or USP10, rescues SG formation."

sparser
"Therefore, although Caprin1 and USP10 both bind G3BP ( xref ; xref ), they have antagonistic effects on SGs."

sparser
"Interaction of Dengue RNA with stress granule proteins G3BP1, USP10 and caprin1 occurs at an adjacent unstructured region of the 3′ UTR ( xref )."
| 6

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [ xref ], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [ xref ]."

sparser
"However, a role for the G3BP1 and G3BP2 interactions with CAPRIN1 and/or USP10 under chronic nutrient starvation has not yet been investigated."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al.2017); SeV Trailer RNA captures TIAR from SG (Iseni et al.2002); West Nile Virus (WNV) and Dengue virus (DENV) 3′-end viral genome captures TIA-1/TIAR (Li et al.2002; Emara and Brinton 2007; Xia et al.2015); DENV 3′-UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al.2011; Reineke et al.2015); JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al.2011)."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al. 2017) ; SeV Trailer RNA captures TIAR from SG (Iseni et al. 2002) ; West Nile Virus (WNV) and Dengue virus (DENV) 3 0 -end viral genome captures TIA-1/TIAR (Li et al. 2002; Emara and Brinton 2007; Xia et al. 2015) ; DENV 3 0 -UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al. 2011; Reineke et al. 2015) ; JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al. 2011) ."

sparser
"Additional experiments demonstrate that, in contrast to DDX6, the SG proteins G3BP1, G3BP2, Caprin1 and USP10 bind to the variable region (VR) in the 3' UTR."

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [171], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [172]."
| 4

sparser
"Validation of the mass spectrometry data confirmed the interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."

sparser
"NCAP showed specific interaction with the SG proteins G3BP1, G3BP2, YTHDF3, USP10 and PKR, and translocated to SGs following oxidative stress and heat shock."

sparser
"NCAP interacts with SG proteins. (A) Interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."

sparser
"Fig. 4: NCAP interacts with SG proteins. (A) Interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."
| 3

sparser
"Since the binding of nsP3 and USP10 to G3BP inhibits SGs, we hypothesized that ICP8 binding might do the same."

sparser
"It was recently identified that a common canonical sequence motif, FGDF, mediates binding of the nsP3 and USP10 proteins to G3BP ."

sparser
"The model might also explain the elimination of binding of both nsP3 and USP10 to G3BP upon mutation of the glycine residue to alanine, which would restrict flexibility and thus hinder adequate positioning of the phenylalanine side-chains in the FGDF motifs."
| 2

sparser
"G3BP1 interacts with p62 and USP10 to inhibit protein aggregation."

sparser
"G3BP1 interacted with p62 and USP10 and inhibited p62/USP10-induced protein aggregation."
TP53 binds USP10 and G3BP1. 2 / 2
| 2

sparser
"Disruption of the interaction of p53 with USP10 by G3BP1 interference leads to suppression of p53 deubiquitination."

sparser
"Disruption of the interaction of p53 with USP10 by G3BP1 interference leads to the suppression of p53 deubiquitination."
USP10 inhibits G3BP1.
| 3
USP10 inhibits G3BP1. 3 / 7
| 3

reach
"This unexpected finding may suggest that binding of the USP10-derived SLiM to NTF2 reduces the propensity of G3BP1 to enter condensates."

reach
"It is also possible that USP10 blocks the helicase function of G3BP, as helicase activity of some proteins, e.g., DDX6 and RCK, has been shown to be required for RNA granule assembly."

reach
"USP10 may prevent G3BP from nucleating SG assembly by locking G3BP in a conformation that influences the NTF2 like domain and mediates or prevents G3BP oligomerization."
USP10 decreases the amount of G3BP1.
| 1
USP10 decreases the amount of G3BP1. 1 / 3
| 1

reach
"However, USP10 KD also reduces expression of G3BP1 and G3BP2 (to 58% and 77% of control values, respectively; XREF_FIG), suggesting that the SG inhibition caused by USP10 KD may be caused by reduced levels of G3BP."
USP10 is modified
45 | 74 2
USP10 is phosphorylated.
45 | 73 2
USP10 is phosphorylated. 10 / 48
| 48

sparser
"However, the relationship between USP10 translocation and stabilization as well as how USP10 phosphorylation affects its stability and localization have not yet been understood."

sparser
"Comparing to the unphosphorylated USP10, phosphorylated USP10 showed much higher activity towards Ub-AMC ( xref )."

sparser
"These results suggest that ATM regulates USP10 phosphorylation after DNA damage."

sparser
"We first phosphorylated USP10 using active AMPK in vitro and checked its activity toward Ub-AMC."

sparser
"DNA damage promotes ataxia telangiectasia mutated (ATM)-dependent phosphorylation of USP10, which undergoes nuclear translocation."

sparser
"Therefore, phosphorylated USP10 can inhibit the growth of tumour cells without inducing mutations in p53 ( xref ), which implies that phosphorylation of USP10 has potential therapeutic effects in tumours."

sparser
"Given USP10 also regulates p53 expression and suppresses tumor cell growth ( Yuan et al., 2010 ), phosphorylation of USP10 under energy stress might contribute to p53 level increase and tumor suppress[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Conversely, MEK-ERK inhibition blocked USP10 phosphorylation and enhanced the USP10-ZEB1 interaction shown to suppress ZEB1-mediated tumor cell migration and metastasis."

sparser
"We further confirmed the role of ATM in USP10 phosphorylation using ATM +/+ or ATM −/− cells."

sparser
"Compared to the unphosphorylated USP10, phosphorylated USP10 showed much higher activity toward Ub-AMC ( Figure 5 A)."
USP10 is phosphorylated on S576. 10 / 11
11 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
USP10 is phosphorylated on T674. 10 / 10
| 10

sparser
"To further clarify that anti-μ antibody and Pam 3 Csk 4 co-treatment promoted T674 phosphorylation of USP10, we first generated an antibody that specifically recognizes the phosphorylation site of USP10 by ."

sparser
"In addition, Luo et al. reported that co-stimulation by BCR and TLR1/2 initiated the AKT-dependent phosphorylation of T674 of USP10; subsequently, USP10 entered the nucleus and stabilized the AID protein (also see Figure 4 and Table 2 ) [72] ."

sparser
"BCR and TLR1/2 activation synergistically inhibited AID degradation in the nucleus through Akt-mediated USP10 T674 phosphorylation and the nuclear translocation USP10 is a cytoplasmic protein characterized by a nucleus-cytoplasm shuttle."

sparser
"To further clarify that anti-μ antibody and Pam 3 Csk 4 co-treatment promoted T674 phosphorylation of USP10, we first generated an antibody that specifically recognizes the phosphorylation site of USP10 by immunizing rabbits with the phosphorylated and sequence-homologous polypeptides (Supplementary Fig. xref )."

sparser
"Interestingly, the BCR-PI3K-Akt signaling pathway activates AKT and phosphorylates the T674 site of USP10 and the TLR1/2 signal, which significantly enhances signal transduction."

sparser
"Luo et al. Fig. 6 BCR and TLR2 activation synergistically inhibited AID degradation in the nucleus through Akt-mediated USP10 T674 phosphorylation and nuclear translocation."

sparser
"Collectively, these results indicated that BCR and TLR2 synergistically enhance Akt activity and promote USP10 T674 phosphorylation located within the NLS domain and, therefore, the nuclear import of USP10, consequently inhibiting the AID degradation in the nucleus."

sparser
"BCR and TLR1/2 activation synergistically inhibited AID degradation in the nucleus through Akt-mediated USP10 T674 phosphorylation and the nuclear translocation."

sparser
"I, j Western blot indicated TLR1/2 and BCR co-stimulation promoted USP10 T674 phosphorylation, but not when AKT inhibitor treatment."

sparser
"AMPKα AMPKα increases its activity by mediating phosphorylation of Ser76 at the USP10 N-terminus. [15] ATM ATM mediates phosphorylation of USP10 at Thr42 and Ser337 and causes USP10's migration into the nucleus. [27] TRAF4 TRAF4 and p53 competitively bind to USP10 and inhibit usP10-mediated p53 deubiquitination. [73] AKT Co-stimulation by BCR and TLR1/2 initiates Akt-dependent phosphorylation of T674 in the USP10 NLS domain. [72] USP13/beclin-1 When USP10 and USP13 interact with beclin-1, the deubiquitination activity of USP10 can be increased. [53] MiR-138 MiR-138 binds to a conserved region of USP10's 3 -UTR and inhibits the accumulation of USP10 mRNA and protein expression level. [31] MicroRNA-191 MicroRNA-191 binds to the 3 -untranslated region of USP10 mRNA, reducing USP10 protein levels. [28] MiR-34a-5p MiR-34a-5p binds to the 3 -untranslated region of USP10 and reduces the expression of USP10. [79] G3BP Direct binding of G3BP to USP10 inhibits its ability to decompose ubiquitin chains. [56] HTLV-1 Tax The central region of Tax interacts with amino acids 727-798 in USP10, inhibiting the activity of USP10. [78] Daam1 negatively regulates USP10's DUB activity. [80] Estrogen Estrogen induces p53 degradation by regulating USP10 activity. [81] Overexpressed FOXO4 inhibits USP10 transcription and protein expression by binding to the bases 1771-1776 in the promoter region TSS of USP10. [82] Zhang et al. indicated that miR-34a-5p acted as a negative regulator of USP10, but the underlying mechanism of the microRNA's action remains largely unclear [79] ."
USP10 is phosphorylated on T42. 7 / 7
| 6 1

sparser
"The translocation and stabilisation of USP10 is regulated by ATM-mediated phosphorylation of USP10 on Thr-42 and Ser-337."

rlimsp
"The translocation and stabilization of USP10 is regulated by ATM -mediated phosphorylation of USP10 at Thr42 and Ser337."

sparser
"A previous study indicated that nuclear translocation of USP10 was regulated by ATMmediated phosphorylation of USP10 at Thr42 and Ser337."

sparser
"AMPKα AMPKα increases its activity by mediating phosphorylation of Ser76 at the USP10 N-terminus. [15] ATM ATM mediates phosphorylation of USP10 at Thr42 and Ser337 and causes USP10's migration into the nucleus. [27] TRAF4 TRAF4 and p53 competitively bind to USP10 and inhibit usP10-mediated p53 deubiquitination. [73] AKT Co-stimulation by BCR and TLR1/2 initiates Akt-dependent phosphorylation of T674 in the USP10 NLS domain. [72] USP13/beclin-1 When USP10 and USP13 interact with beclin-1, the deubiquitination activity of USP10 can be increased. [53] MiR-138 MiR-138 binds to a conserved region of USP10's 3 -UTR and inhibits the accumulation of USP10 mRNA and protein expression level. [31] MicroRNA-191 MicroRNA-191 binds to the 3 -untranslated region of USP10 mRNA, reducing USP10 protein levels. [28] MiR-34a-5p MiR-34a-5p binds to the 3 -untranslated region of USP10 and reduces the expression of USP10. [79] G3BP Direct binding of G3BP to USP10 inhibits its ability to decompose ubiquitin chains. [56] HTLV-1 Tax The central region of Tax interacts with amino acids 727-798 in USP10, inhibiting the activity of USP10. [78] Daam1 negatively regulates USP10's DUB activity. [80] Estrogen Estrogen induces p53 degradation by regulating USP10 activity. [81] Overexpressed FOXO4 inhibits USP10 transcription and protein expression by binding to the bases 1771-1776 in the promoter region TSS of USP10. [82] Zhang et al. indicated that miR-34a-5p acted as a negative regulator of USP10, but the underlying mechanism of the microRNA's action remains largely unclear [79] ."

sparser
"A previous study indicated that nuclear translocation of USP10 was regulated by ATM-mediated phosphorylation of USP10 at Thr42 and Ser337. xref We compared the effects of T674E USP10 and T42E-S337D-USP10 on nuclear AID degradation using a dendra2 decay assay."

sparser
"In the case of DNA damage, the phosphorylation of USP10 at Thr42 and Ser337 mediated by ATM is essential for its translocation to the nucleus, where USP10 induces the deubiquitination of p53 and makes p53 work as a tumor suppressor (Fig. xref ). xref "

sparser
"Phosphorylation of USP10 on Thr42 and Ser337 triggers its translocation, enabling the deubiquitination, stabilization and activation of p53."
USP10 is phosphorylated on S365. 6 / 6
6 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
USP10 is phosphorylated on S337. 6 / 6
| 6

sparser
"AMPKα AMPKα increases its activity by mediating phosphorylation of Ser76 at the USP10 N-terminus. [15] ATM ATM mediates phosphorylation of USP10 at Thr42 and Ser337 and causes USP10's migration into the nucleus. [27] TRAF4 TRAF4 and p53 competitively bind to USP10 and inhibit usP10-mediated p53 deubiquitination. [73] AKT Co-stimulation by BCR and TLR1/2 initiates Akt-dependent phosphorylation of T674 in the USP10 NLS domain. [72] USP13/beclin-1 When USP10 and USP13 interact with beclin-1, the deubiquitination activity of USP10 can be increased. [53] MiR-138 MiR-138 binds to a conserved region of USP10's 3 -UTR and inhibits the accumulation of USP10 mRNA and protein expression level. [31] MicroRNA-191 MicroRNA-191 binds to the 3 -untranslated region of USP10 mRNA, reducing USP10 protein levels. [28] MiR-34a-5p MiR-34a-5p binds to the 3 -untranslated region of USP10 and reduces the expression of USP10. [79] G3BP Direct binding of G3BP to USP10 inhibits its ability to decompose ubiquitin chains. [56] HTLV-1 Tax The central region of Tax interacts with amino acids 727-798 in USP10, inhibiting the activity of USP10. [78] Daam1 negatively regulates USP10's DUB activity. [80] Estrogen Estrogen induces p53 degradation by regulating USP10 activity. [81] Overexpressed FOXO4 inhibits USP10 transcription and protein expression by binding to the bases 1771-1776 in the promoter region TSS of USP10. [82] Zhang et al. indicated that miR-34a-5p acted as a negative regulator of USP10, but the underlying mechanism of the microRNA's action remains largely unclear [79] ."

sparser
"A previous study indicated that nuclear translocation of USP10 was regulated by ATMmediated phosphorylation of USP10 at Thr42 and Ser337."

sparser
"Phosphorylation of USP10 on Thr42 and Ser337 triggers its translocation, enabling the deubiquitination, stabilization and activation of p53."

sparser
"The translocation and stabilisation of USP10 is regulated by ATM-mediated phosphorylation of USP10 on Thr-42 and Ser-337."

sparser
"In the case of DNA damage, the phosphorylation of USP10 at Thr42 and Ser337 mediated by ATM is essential for its translocation to the nucleus, where USP10 induces the deubiquitination of p53 and makes p53 work as a tumor suppressor (Fig. xref ). xref "

sparser
"A previous study indicated that nuclear translocation of USP10 was regulated by ATM-mediated phosphorylation of USP10 at Thr42 and Ser337. xref We compared the effects of T674E USP10 and T42E-S337D-USP10 on nuclear AID degradation using a dendra2 decay assay."
USP10 is phosphorylated on S370. 6 / 6
6 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
USP10 is phosphorylated on S76. 4 / 4
| 3 1

sparser
"These results suggest that S76 phosphorylation of USP10 increases the deubiquitinase activity of USP10."

rlimsp
"Under energy stress, USP10 activity in turn is enhanced through AMPK-mediated phosphorylation of Ser76 of USP10."

sparser
"We further tested whether phosphorylation of S76 on USP10 could affect the ubiquitination and activation of AMPKα in cells."

sparser
"Under energy stress, USP10 activity in turn is enhanced through AMPK-mediated phosphorylation of Ser76 of USP10."
USP10 is phosphorylated on S211. 4 / 4
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
USP10 is phosphorylated on Y364. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
USP10 is phosphorylated on S547. 2 / 2
2 |

No evidence text available

No evidence text available
USP10 is phosphorylated on T208. 2 / 2
2 |

No evidence text available

No evidence text available
USP10 is phosphorylated on T100. 2 / 2
2 |

No evidence text available

No evidence text available
USP10 is phosphorylated on S563. 2 / 2
2 |

No evidence text available

No evidence text available
USP10 is phosphorylated on T216. 2 / 2
2 |

No evidence text available

No evidence text available
USP10 is phosphorylated on S718. 1 / 1
1 |

No evidence text available
USP10 is phosphorylated on S355. 1 / 1
1 |

No evidence text available
USP10 is phosphorylated on T205. 1 / 1
1 |

No evidence text available
USP10 is phosphorylated on S14. 1 / 1
1 |

No evidence text available
USP10 is phosphorylated on Y77. 1 / 1
1 |

No evidence text available
USP10 is ubiquitinated.
| 1
USP10 is ubiquitinated. 1 / 1
| 1

sparser
"G3BP1 has been reported to suppress p62&USP10 complex ubiquitination [ xref ], indicating that G3BP1 may regulate protein expression by inhibiting USP10 ubiquitination."
USP10 affects SIRT6
1 1 | 2 33 13
USP10 binds SIRT6.
1 | 14 13
1 | 14 12

sparser
"Moreover, the specific interaction between the endogenous USP10 and SIRT6 was confirmed in HCT116 colon cancer cells because SIRT6 protein was detected in the immunoprecipitates with anti-USP10 antibody, but not with the control mouse IgG ( xref )."

reach
"We demonstrated the interaction between USP10 and Sirt6 in AB induced CH phenotype by USP10 knockout or transgenic mice, but no Sirt6 knockout or transgenic mice."

reach
"As indicated in XREF_FIG, USP10 interacts with SIRT6 but not with any of the other six sirtuin family proteins."

reach
"For example, in colon cancer, USP10 interacted with and stabilized SIRT6 through suppressing SIRT6 ubiquitination [25]."

sparser
"USP10 interacts with SIRT6."

reach
"For example, in colon cancer, USP10 interacted with and stabilized SIRT6 through inhibiting SIRT6’s ubiquitination, suppressing proliferation [25]."

sparser
"Collectively, these data demonstrate that USP10 can interact with Sirt6 and promote the ubiquitination of Sirt6 in cardiomyocytes and then inhibit the downstream Akt signaling pathway and cardiac hypertrophy."

sparser
"We demonstrated the interaction between USP10 and Sirt6 in AB‐induced CH phenotype by USP10 knockout or transgenic mice, but no Sirt6 knockout or transgenic mice."

No evidence text available

sparser
"As indicated in xref , USP10 interacts with SIRT6 but not with any of the other six sirtuin family proteins."
| 1

sparser
"Furthermore, USP10 physically binds to sirtuin 6 (Sirt6) and Sirt6 mediates the effect of USP10 on the regulation of CH."
USP10 inhibits SIRT6.
| 2
USP10 inhibits SIRT6. 2 / 14
| 2

reach
"Moreover, USP10 attenuates sepsis-induced oxidative stress in renal tissues by increasing SIRT6 stability and activating the NRF2 /ARE signaling pathway."

reach
"Accordingly, inactivation of USP10 reduced Sirt6 abundance and stability and diminished Sirt6 induced downstream signaling in cardiomyocytes."
USP10 activates SIRT6.
| 2 4
USP10 activates SIRT6. 6 / 9
| 2 4

reach
"Furthermore, USP10 directly regulates Sirt6 during the pathogenesis of CH."

reach
"These studies suggest the possibility that the association with RBM5-AS1 may reinforce USP10 dependent suppression of SIRT6 degradation."
| PMC

reach
"In contrast, knockdown of USP10 but not USP22 in RKO cells promoted SIRT6 degradation."

eidos
"Studies have shown that the protective effect of USP10 against organ injury is related to SIRT6 ."

eidos
"It was shown that knockdown of USP10 decreased Sirt6 expression , whereas overexpression of USP10 increased Sirt6 protein expression in primary cardiomyocytes after Ang II stimulation ( Figure 6C and 6D ) ."

reach
"Therefore, it seems that USP10 mediated SIRT6 stabilization is largely involved in regulating c-Myc transcriptional activity."
USP10 deubiquitinates SIRT6.
1 | 7
USP10 deubiquitinates SIRT6. 8 / 8
1 | 7

reach
"As previously mentioned, the peptidase USP10 de-ubiquitinates SIRT6, thereby protecting it from proteasomal degradation."
| PMC

reach
"USP10 suppresses SIRT6 ubiquitination to protect SIRT6 from proteasomal degradation."

reach
"In addition, Lin et al. demonstrated that USP10 enhanced the stability of SIRT6 through interacting with its N-terminal regulatory domain and deubiquitinating SIRT6, and then inhibited the transcriptional activity of the c-Myc oncogene through SIRT6 [25]."

reach
"USP10 deubiquitinates and stabilizes the tumor suppressor p53, and SIRT6."

reach
"Second, USP10 suppresses SIRT6 ubiquitination and protects SIRT6 from proteasome mediated degradation."

reach
"USP10 deubiquitinates and stabilizes SIRT6, which amplifies the tumor-suppressive activity of SIRT6."

reach
"USP10 negatively regulates SIRT6 ubiquitination."
USP10 increases the amount of SIRT6.
| 4
USP10 increases the amount of SIRT6. 4 / 4
| 4

reach
"Conversely, shRNA mediated knockdown of USP10 decreased the endogenous SIRT6 protein levels without affecting SIRT6 mRNA levels (XREF_FIG)."

reach
"It was shown that knockdown of USP10 decreased Sirt6 expression, whereas overexpression of USP10 increased Sirt6 protein expression in primary cardiomyocytes after Ang II stimulation."

reach
"Western blot assay showed that LPS resulted in decreased Sirt6 and increased Nrf2 and HO-1 levels, whereas USP10 overexpression led to an increased expression of Sirt6, Nrf2 and HO-1 in HK-2 cells induced by LPS (Fig. 6 A)."

reach
"Furthermore, our results demonstrated that USP10 overexpression increased Sirt6, Nrf2 and HO-1 levels, but were reduced following Sirt6 knockdown (Fig. 6B)."
USP10 ubiquitinates SIRT6.
| 2
USP10 ubiquitinates SIRT6. 2 / 2
| 2

reach
"Lin et al. found that USP10 antagonizes the transcriptional activity of c‐Myc by deubiquitinating and stabilizing SIRT6, to inhibit cell growth and tumor formation in colon cancers (Lin et al., 2013)."

reach
"Mechanistically, we demonstrated that USP10 activation and interaction with Sirt6 in response to angiotensin II led to a marked increase in the ubiquitination of Sirt6 and resulted in Akt signaling downregulation and attenuation of cardiomyocyte hypertrophy."
TP53 affects USP10
7 | 21 33
TP53 binds USP10.
7 | 17 32
7 | 17 27

sparser
"In unstressed cells, it was shown that USP10 binds, deubiquitinates and stabilizes p53 in the cytoplasm, antagonizing Mdm2-mediated p53 nuclear export and degradation."

No evidence text available

sparser
"USP10 is also associated with regulation of p53 localization and stability, however, unlike USP2 and USP7, it does not interact with MDM2 [ xref ]."

No evidence text available

reach
"The inhibition of the proline hydroxylase PHD3 could significantly reduce the binding of USP10 to p53 [35]."

sparser
"G3BP1 disrupts the interaction of USP10 with p53."

reach
"IGF2BP3 (insulin-like growth factor 2 mRNA binding protein 3) inhibits p53 expression through disrupting the interaction between USP10 and p53 and promotes lung cancer progression [37]."

sparser
"These results suggest that G3BP1 interrupts the interaction of USP10 with p53 by its own interaction with USP10."

sparser
"Similar to what we observed with USP7, inhibition of hydroxylase activity diminished the interaction between p53 and endogenous USP10."

reach
"In other words, in the presence of p53, USP10 largely binds p53 and stabilizes p53 under the stress conditions."
TP53 binds USP10 and USP7. 3 / 3
| 3

sparser
"For example, USP7 and USP10 bind to and deubiquitylate their substrate p53 to mediate its role for suppression of cell propagation upon cellular stresses by counteracting its degradation xref – xref ."

sparser
"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced under DMOG treatment ( xref A)."

sparser
"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced un[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
TP53 binds USP10 and G3BP1. 2 / 2
| 2

sparser
"Disruption of the interaction of p53 with USP10 by G3BP1 interference leads to suppression of p53 deubiquitination."

sparser
"Disruption of the interaction of p53 with USP10 by G3BP1 interference leads to the suppression of p53 deubiquitination."
TP53 inhibits USP10.
| 2
TP53 inhibits USP10. 2 / 2
| 2

reach
"We further found miR-138 overexpression inhibits TP53-dependent transcription by repressing USP10 and abrogates TP53-dependent cell apoptosis and cell cycle arrest."

reach
"Furthermore, USP10 knockdown mediated cell cycle progression was partially rescued by either SIRT6 or p53 co-expression."
TP53 activates USP10.
| 2
TP53 activates USP10. 2 / 2
| 2

reach
"Since USP10 mediates the deubiquitination of p53, regulation of the deubiquitination activity of USP10 by Beclin1 likely plays an important role in tumor suppression."

reach
"Downregulation of p53 abolished the inhibitory effect of USP10 on tumor growth (XREF_FIG and data not shown), confirming that USP10 inhibits cancer cell growth by stabilizing p53."
TP53 ubiquitinates USP10.
| 1
TP53 ubiquitinates USP10. 1 / 1
| 1

sparser
"In the 786-O RCC cell line, USP10 overexpression inhibits MDM2-mediated mutp53 ubiquitination leading to stabilization of mutp53, while USP10 downregulation increases mutp53 ubiquitination to decrease its stability."
ATM affects USP10
2 | 23 25
ATM phosphorylates USP10.
2 | 22 24
ATM phosphorylates USP10. 10 / 29
| 13 16

sparser
"We further examined the functional significance of USP10 phosphorylation by ATM."

reach
"Following DNA damage, ATM phosphorylates USP10, resulting in stabilization of USP10 and allowing it to more efficiently deubiquitinate and recycle cytoplasmic p53 back to the nucleus [XREF_BIBR]."

sparser
"It is possible that USP10 phosphorylation by ATM hinders USP10 nuclear export and shields it from degradation in the cytoplasm."

reach
"Upon DNA damage, USP10 is phosphorylated by ATM, after which it is re-localized to the nucleus where p53 de-ubiquitination occurs, which is the reverse of the function of residual MDM2, which ubiquitinates p53."

sparser
"Upon induction by DNA damage, a fraction of USP10 is phosphorylated by ATM and translocates to the nucleus where it deubiquitinates p53."

reach
"Upon induction by DNA damage, a fraction of USP10 is phosphorylated by ATM and translocates to the nucleus where it deubiquitinates p53."

reach
"Importantly, USP10 can be phosphorylated by the ATM kinase, leading to its stabilization and nuclear translocation."

reach
"Importantly, USP10 can be phosphorylated by the ATM kinase, leading to its stabilization and nuclear translocation."

reach
"It is possible that USP10 phosphorylation by ATM hinders USP10 nuclear export and shields it from degradation in the cytoplasm."

sparser
"Importantly, USP10 can be phosphorylated by the ATM kinase, leading to its stabilization and nuclear translocation."
ATM phosphorylates USP10 on S337. 10 / 11
1 | 5 4

reach
"ATM phosphorylates USP10 on Thr 42 and Ser 337, and this event is required for the stabilization of USP10 and its translocation into nucleus after DNA damage."

reach
"In the case of DNA damage, the phosphorylation of USP10 at Thr42 and Ser337 mediated by ATM is essential for its translocation to the nucleus, where USP10 induces the deubiquitination of p53 and makes p53 work as a tumor suppressor."

No evidence text available

reach
"Mechanistically, the phosphorylation of Thr42 and Ser337 of USP10 by ATM is required for USP10 translocation and stabilization."

sparser
"ATM phosphorylates USP10 on Thr 42 and Ser 337 , and this event is required for the stabilization of USP10 and its translocation into nucleus after DNA damage."

sparser
"Yuan et al. demonstrated that ATM could phosphorylate USP10 at Thr42 and Ser337 upon the DNA-damage response and USP10 was translocated into the nucleus, where the N-terminus of USP10 (amino acids 1-100) binds to p53 and inhibits its ubiquitination [27] ."

sparser
"Mechanistically, the phosphorylation of Thr42 and Ser337 of USP10 by ATM is required for USP10 translocation and stabilization."

sparser
"The translocation and stabilization of USP10 is regulated by ATM -mediated phosphorylation of USP10 at Thr42 and Ser337."

reach
"The translocation and stabilization of USP10 is regulated by ATM -mediated phosphorylation of USP10 at Thr42 and Ser337."

reach
"Yuan et al. demonstrated that ATM could phosphorylate USP10 at Thr42 and Ser337 upon the DNA-damage response and USP10 was translocated into the nucleus, where the N-terminus of USP10 (amino acids 1–100) binds to p53 and inhibits its ubiquitination [27]."
ATM phosphorylates USP10 on T42. 9 / 10
1 | 4 4

sparser
"The translocation and stabilization of USP10 is regulated by ATM -mediated phosphorylation of USP10 at Thr42 and Ser337."

reach
"Yuan et al. demonstrated that ATM could phosphorylate USP10 at Thr42 and Ser337 upon the DNA-damage response and USP10 was translocated into the nucleus, where the N-terminus of USP10 (amino acids 1–100) binds to p53 and inhibits its ubiquitination [27]."

sparser
"ATM phosphorylates USP10 on Thr 42 and Ser 337 , and this event is required for the stabilization of USP10 and its translocation into nucleus after DNA damage."

sparser
"Mechanistically, the phosphorylation of Thr42 and Ser337 of USP10 by ATM is required for USP10 translocation and stabilization."

reach
"In the case of DNA damage, the phosphorylation of USP10 at Thr42 and Ser337 mediated by ATM is essential for its translocation to the nucleus, where USP10 induces the deubiquitination of p53 and makes p53 work as a tumor suppressor."

reach
"ATM phosphorylates USP10 on Thr 42 and Ser 337, and this event is required for the stabilization of USP10 and its translocation into nucleus after DNA damage."

No evidence text available

sparser
"Yuan et al. demonstrated that ATM could phosphorylate USP10 at Thr42 and Ser337 upon the DNA-damage response and USP10 was translocated into the nucleus, where the N-terminus of USP10 (amino acids 1-100) binds to p53 and inhibits its ubiquitination [27] ."

reach
"Mechanistically, the phosphorylation of Thr42 and Ser337 of USP10 by ATM is required for USP10 translocation and stabilization."
ATM activates USP10.
| 1 1
ATM activates USP10. 2 / 2
| 1 1

reach
"As shown, both AKT and ATM increase the activity of USP10."

sparser
"ROS-induced stress promotes ATM-dependent USP10 activation."
| 5 36 8
| 5 30 8
| 5 30 8

reach
"USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells."

reach
"Collectively, these results indicate that USP10 inhibits cytokine deprivation induced apoptosis of IVC-LSK cells in a deubiquitinase dependent but ROS independent manner."

reach
"XREF_FIG, overexpression of either HDAC6 or USP10 in USP10 knockdown cells significantly increased cell survival post-cisplatin treatment and reduced apoptosis as shown by the level of cleaved PARP-1."

reach
"USP10 also has deubiquitinase independent functions, such that USP10 inhibits apoptosis by reducing reactive oxygen species (ROS) production induced by an oxidative stress inducer arsenite."

sparser
"We previously reported that USP10 inhibits oxidant-induced apoptosis by reducing ROS production in mouse embryonic fibroblasts (MEFs) ( xref )."

reach
"USP10 alleviates sepsis-induced renal dysfunction and reduces renal tubular epithelial cell apoptosis and oxidative stress."

eidos
"Consistently , in our study , we also demonstrated that USP10 inhibited hepatocyte inflammation and apoptosis in hepatocytes following H / R stimulation ( Figure 5 ) ."

sparser
"USP10 also has deubiquitinase-independent functions, such that USP10 inhibits apoptosis by reducing reactive oxygen species (ROS) production induced by an oxidative stress inducer arsenite ( xref )."

eidos
"USP10 inhibits apoptosis by two distinct mechanisms : deubiquitinase-independent / ROS-dependent and deubiquitinase-dependent / ROS-independent ."

reach
"Additionally, USP10 overexpression inhibited hepatocyte inflammation and apoptosis in hepatic I/R injury in vitro and in vivo."
| 6

reach
"Moreover, USP10 was found to reduce oxidative stress, and abated LPS-induced renal tubular epithelial cell injury and apoptosis."

reach
"USP10 promotes proliferation and migration and inhibits apoptosis of endometrial stromal cells in endometriosis through activating the Raf-1/MEK/ERK pathway."

reach
"USP10 Heterozygote mice exhibited exacerbated hepatic I/R injury, as evidenced by enhanced liver inflammation via the NF-kappaB signalling pathway and increased hepatocyte apoptosis."

reach
"Their results show that the depletion of USP10 in A549 increased cell survival and decreased apoptosis through destabilizing MSH2 [32]."

reach
"In conclusion, these results suggest that USP10 promotes proliferation and migration and inhibits apoptosis of endometrial stromal cells in endometriosis through activating the Raf-1/MEK/ERK pathway."

reach
"USP10-knockout indicated that USP10, through augmenting formation of SGs, reduced reactive oxygen species (ROS) production and inhibited ROS dependent apoptosis."
| PMC
USP10 affects CAPRIN1
| 10 36
USP10 binds CAPRIN1.
| 8 36
| 8 19

reach
"However, it is important to note that the interactions between G3BP1 and USP10 or Caprin1 do not fully dictate SG disassembly or assembly, as two different laboratories recently demonstrated that the unstressed G3BP1 interactome significantly overlaps the interactome of G3BP1 during stress [XREF_BIBR, XREF_BIBR]."

sparser
"Subsequent work demonstrated that in addition to homo- and hetero-dimerization, G3BP1 and 2 interact with USP10 and Caprin1 [ xref , xref , xref , xref ]."

reach
"Impact of G3BP1 F33W mutation on USP10 and Caprin1 peptide binding."
| DOI

reach
"G3BP mediated SG assembly is inhibited by serine 149 phosphorylation, regulated by mutually exclusive binding of Caprin1 and USP10, and requires its RGG region that allows interactions with 40S subunits."

sparser
"G3BP1-F33W is SG competent despite its inability to bind Caprin1 or USP10, suggesting that G3BP is only regulated by Caprin1/USP10."

reach
"As a result, this disjunction between USP10 and Caprin1 binding may well reflect differences in the motif peptides, since the USP10 FGDF motif contains two phenylalanines, whereas the Caprin1 FIQDSMLD motif contains only one."
| DOI

sparser
"SG formation is rescued in these cells by expression of either wild-type G3BP, or G3BP containing a point mutation that renders it unable to bind USP10 or Caprin1."

sparser
"Nancy presented preliminary data indicating differential regulation of SG nucleation by these G3BP1 binding partners, whereby Caprin1 and USP10 bind the G3BP-NTF2-like domain in a competitive manner, with opposite results; G3BP1:Caprin1 complexes promote SG formation, whereas G3BP1:USP10 complexes inhibit SG formation."

sparser
"In addition, the acetylation-mimicking mutation K376Q impaired the RNA-dependent interaction of G3BP1 with PABP1, but its RNA-independent interactions with Caprin-1 and USP10 were little affected."

sparser
"Recently, Kedersha and colleagues reported that SG assembly is finely regulated by the binding of G3BPs to Caprin1 or USP10 [ xref ]."
| 7

sparser
"However, a recent study suggests that G3BP proteins interact with Caprin1 and USP10 but that they are mutually exclusive allowing the binding of these proteins to serve as a switch for SG formation depending on the binding partner interacting with G3BP1."

sparser
"However, it is important to note that the interactions between G3BP1 and USP10 or Caprin1 do not fully dictate SG disassembly or assembly, as two different laboratories recently demonstrated that the unstressed G3BP1 interactome significantly overlaps the interactome of G3BP1 during stress [ xref , xref ]."

sparser
"USP10 and Caprin1 antagonistically bind G3BP to regulate SG formation."

sparser
"Our results indicated that N expression abrogates G3BP1 interaction with the SG proteins CAPRIN1 and USP10 ( Figure 2C ), reinforcing the idea that N inhibits the formation of SGs in host cells by sequestering G3BPs away from their interacting SG-nucleating proteins."

sparser
"Rescue experiments using G3BP1 mutants show that phosphomimetic G3BP1-S149E fails to rescue SG formation, whereas G3BP1-F33W, a mutant unable to bind G3BP partner proteins Caprin1 or USP10, rescues SG formation."

sparser
"Therefore, although Caprin1 and USP10 both bind G3BP ( xref ; xref ), they have antagonistic effects on SGs."

sparser
"Interaction of Dengue RNA with stress granule proteins G3BP1, USP10 and caprin1 occurs at an adjacent unstructured region of the 3′ UTR ( xref )."
| 6

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [ xref ], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [ xref ]."

sparser
"However, a role for the G3BP1 and G3BP2 interactions with CAPRIN1 and/or USP10 under chronic nutrient starvation has not yet been investigated."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al.2017); SeV Trailer RNA captures TIAR from SG (Iseni et al.2002); West Nile Virus (WNV) and Dengue virus (DENV) 3′-end viral genome captures TIA-1/TIAR (Li et al.2002; Emara and Brinton 2007; Xia et al.2015); DENV 3′-UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al.2011; Reineke et al.2015); JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al.2011)."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al. 2017) ; SeV Trailer RNA captures TIAR from SG (Iseni et al. 2002) ; West Nile Virus (WNV) and Dengue virus (DENV) 3 0 -end viral genome captures TIA-1/TIAR (Li et al. 2002; Emara and Brinton 2007; Xia et al. 2015) ; DENV 3 0 -UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al. 2011; Reineke et al. 2015) ; JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al. 2011) ."

sparser
"Additional experiments demonstrate that, in contrast to DDX6, the SG proteins G3BP1, G3BP2, Caprin1 and USP10 bind to the variable region (VR) in the 3' UTR."

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [171], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [172]."
| 2

sparser
"Finally, acetylation of Lysine-376 (K376) in the RRM domain, impairs the RNA binding function of G3BP1 as well as its interaction with PABP1, USP10 or Caprin1[ xref ]."

sparser
"As previously discussed, acetylation of K376 of G3BP1 by the CBP/P300 acetylase is a key modification impeding SGs assembly by impairing its RNA binding function, as well as its interaction with PABP1, USP10 or Caprin1[ xref ]."
| 2

sparser
"The crystal structures of the nuclear transport factor 2-like (NTF2L) domain of G3BP1 in complex with the G3BP1-interacting motif (GIM) of Caprin-1 and USP10 show that both GIMs bind to the same hydrophobic pocket of G3BP1."

sparser
"These are the same domains that are necessary for the formation of SGs under conditions of cellular stress, the NTF2-like domain is needed for homodimerisation as well as binding to caprin-1 and USP10, the positive and negative regulators of SGs, while the RGG region mediates binding to the 40S ribosomal subunits [ xref ]."
USP10 activates CAPRIN1.
| 2
USP10 activates CAPRIN1. 2 / 2
| 2

reach
"G3BP-binding and SG-inhibiting variants of USP10 and SFV-nsP3 (XREF_FIG, lanes 3, 5, 6, and 8) selectively decrease G3BP1/2 and Caprin1 B-isox precipitation without affecting that of TIA-1, TIAR, TDP43, or PABP."

reach
"Interestingly, our previous map of human protein complexes (Drew et al., 2017a) suggests that the known complex of G3BP, CAPRIN, and USP10 (Kedersha et al., 2016) also physically interacts with NUFIP2, leading us to suggest the name CapGUN (i.e., CAPRIN1, G3BP2, USP10, NUFIP2)."
AMPK affects USP10
| 23 22
AMPK phosphorylates USP10.
| 18 15
AMPK phosphorylates USP10. 10 / 25
| 13 12

reach
"The maximal stoichiometry of phosphorylation of USP10 peptide by AMPK was 0.46 +/-0.04 mol and mol, which was also higher than that of SAMS (0.35 +/-0.02 mol and mol) (XREF_FIG)."

sparser
"We first tested whether USP10 could be phosphorylated by AMPK using a pan-AMPK substrate antibody."

reach
"To further confirm the phosphorylation of USP10 by AMPK, we compared the phosphorylation of a synthesized USP10 S76 peptide with the SAMS peptide, a known AMPK substrate in an in vitro kinase assay."

reach
"The maximal stoichiometry of phosphorylation of USP10 peptide by AMPK was 0.46 +/- 0.04 mol and mol, which was also higher than that of SAMS (0.35 +/- 0.02 mol and mol)."

sparser
"We found that AMPK specifically phosphorylated USP10 but not GST in vitro ( Figure 4 C), suggesting that USP10 is an AMPK substrate."

sparser
"The maximal stoichiometry of phosphorylation of USP10 peptide by AMPK was 0.46 ± 0.04 mol/mol, which was also higher than that of SAMS (0.35 ± 0.02 mol/mol) ( Figure 4 E)."

sparser
"Phosphorylation of USP10 by AMPK increases its activity."

sparser
"After the S76 is mutated to Ala, the phosphorylation of USP10 by AMPK in vitro was totally abolished ( xref )."

reach
"After the S76 is mutated to Ala, the phosphorylation of USP10 by AMPK in vitro was totally abolished (XREF_FIG)."

sparser
"To further confirm the phosphorylation of USP10 by AMPK, we compared the phosphorylation of a synthesized USP10 S76 peptide with the SAMS peptide, a known AMPK substrate in an in vitro kinase assay ( [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
AMPK phosphorylates USP10 on S76. 8 / 8
| 5 3

sparser
"Furthermore, under energy stress, AMPK phosphorylates USP10 at Ser76 which increases its hydrolase activity( xref )."

reach
"Together, these results suggested that AMPK phosphorylates USP10 at S76, which activates USP10 and facilitates further activation of AMPK under energy stress, forming a feedforward loop."

reach
"Reversely, AMPK could also phosphorylate USP10 Ser76 residue, both of which construct a feedforward loop and thus help the exhaustive cells to survive extreme environments [XREF_BIBR]."

reach
"Furthermore, under energy stress, AMPK phosphorylates USP10 at Ser76 which increases its hydrolase activity."

reach
"Together, these results suggested that AMPK phosphorylates USP10 at S76, which activates USP10 and facilitates further activation of AMPK under energy stress, forming a feedforward loop.Given the impo[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"These results suggest that AMPK phosphorylates USP10 at Ser 76."

reach
"These results suggest that AMPK phosphorylates USP10 at Ser 76."

sparser
"Together, these results suggested that AMPK phosphorylates USP10 at S76, which activates USP10 and facilitates further activation of AMPK under energy stress, forming a feedforward loop."
AMPK binds USP10.
| 3 6
| 3 6

reach
"These results suggest that USP10 deubiquitinase activity is required for its effects in AMPK activation and cellular metabolism.We then explored the mechanism by which USP10 modulates AMPK activation,[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"USP10 interacts with AMPK and deubiquitinates AMPKα."

reach
"USP10 interacts with AMPK and deubiquitinates AMPKalpha."

sparser
"We then explored the mechanism by which USP10 modulates AMPK activation, first by examining whether USP10 directly interacts with AMPK."

reach
"We then explored the mechanism by which USP10 modulates AMPK activation, first by examining whether USP10 directly interacts with AMPK."

sparser
"Thus, USP10 and AMPK form a key feedforward loop ensuring amplification of AMPK activation in response to fluctuation of cellular energy status."

sparser
"Using recombinant proteins, we found that USP10 directly interacted with AMPK α1 and α2 but not the other AMPK subunits ( xref )."

sparser
"USP10 and AMPK can form a key feedforward loop that ensures amplification of AMPK activation in response to fluctuations in cellular energy status ( xref )."

sparser
"Using recombinant proteins, we found that USP10 directly interacted with AMPK α1 and α2 but not the other AMPK subunits ( Figure 2 A)."
AMPK activates USP10.
| 2 1
AMPK activates USP10. 3 / 3
| 2 1

sparser
"After AMPK is partially activated, AMPK activates USP10 to remove the inhibitory ubiquitination on itself, forming a positive feedforward loop."

reach
"In this thorough study, AMPK was also shown to activate USP10 via phosphorylation of S76, indicating the presence of a positive feedforward loop important for maintenance of metabolic homeostasis in liver."

reach
"After AMPK is partially activated, AMPK activates USP10 to remove the inhibitory ubiquitination on itself, forming a positive feedforward loop."
USP10 affects CFTR
3 1 | 27 9
USP10 binds CFTR.
3 | 7 9
3 | 7 9

sparser
"Here, we extend our previous study, conducted using a stably transformed airway epithelial cell line, by demonstrating that USP10 associates with CFTR in primary cultures of polarized human bronchial epithelial cells."

sparser
"Moreover, USP10 interacted with CFTR in the early endosomes, an important requirement for a DUB to regulate a target protein."

reach
"XREF_BIBR, XREF_BIBR Thus, if USP10 regulates the deubiquitination of CFTR, we would predict that USP10 should interact with CFTR in the early endosomal compartment of HBE cells."

sparser
"The ability of USP10 to deubiquitinate target proteins requires interaction between USP10 and its substrate. xref , xref Thus, if USP10 regulates the deubiquitination of CFTR, we would predict that USP10 should interact with CFTR in the early endosomal compartment of HBE cells."

reach
"Three observations suggest that Cif may inhibit USP10 activity by stabilizing the interaction between USP10 and G3BP1, which inhibits USP10 DUB activity, and also reduces the interaction between USP10 and CFTR."

sparser
"As shown in xref , USP10 interacts with CFTR in the early endosomes isolated from airway epithelial cells, and Cif reduces this interaction by 50±6%."

reach
"Thus, studies were conducted to determine if Cif inhibits USP10 activity by stabilizing the interaction between USP10 and G3BP1, and by reducing the interaction between USP10 and CFTR."

sparser
"In the current study, we show localization and activity of USP10 in the early endosomal compartment of primary bronchial epithelial cells, as well as an interaction between CFTR and USP10 in this compartment."

sparser
"Thus, USP10 and CFTR interact in the early endosomal compartment of polarized primary human bronchial epithelial cells."

sparser
"Thus, studies were conducted to determine if Cif inhibits USP10 activity by stabilizing the interaction between USP10 and G3BP1, and by reducing the interaction between USP10 and CFTR."
USP10 deubiquitinates CFTR.
1 | 10
USP10 deubiquitinates CFTR. 10 / 11
1 | 10

reach
"Inhibition of USP10 suppresses post-endocytic de-ubiquitination of CFTR, and the cell targets the ion channel to the lysosome rather than recycling it into the apical membrane."

reach
"USP10 decreases the ubiquitination of CFTR, whereas the knockdown of USP10 promotes the ubiquitination and reduction of CFTR at the cell surface [XREF_BIBR]."
| PMC

reach
"USP10 deubiquitinates CFTR in endosomes, thereby increasing the probability of endocytic recycling, which in turn helps to maintain the abundance of CFTR at the plasma membrane."
| DOI

reach
"Functionally, Cif stabilizes an intracellular complex of a DUB (USP10) and G3BP1, preventing deubiquitylation of CFTR, which is required for recycling the receptor to the plasma membrane XREF_BIBR."

reach
"We previously reported that the DUB USP10 deubiquitinates CFTR in early endosomes of human airway epithelial cells XREF_BIBR."

reach
"Cif stabilizes a G3BP1:USP10 complex in lung cells which renders USP10 inactive and thus unable to deubiquitinate CFTR (20, 36, 71) ."
| DOI

reach
"Although it has been shown that USP10 deubiquitinates and degrades CFTR [64], Pesce et al. found that spautin-1 could not affect the expression of CFTR, indicating that spautin-1 affected CF progression in a USP10-independent manner."

reach
"In a recent study, we demonstrated that USP10, a host cell DUB, deubiquitinates CFTR in endosomes, thereby reducing the lysosomal degradation of CFTR, and maintaining cell and plasma membrane CFTR XREF_BIBR."

reach
"The ability of USP10 to efficiently deubiquitinate CFTR in the early endosome allows for efficient endocytic recycling of CFTR and thus the long half-life of CFTR (approximately 8-24 h in polarized human airway cells) in the plasma membrane."
USP10 decreases the amount of CFTR.
| 5
USP10 decreases the amount of ubiquitinated CFTR. 3 / 3
| 3

reach
"Overexpressing USP-10 decreased the amount of ubiquitinated CFTR and increased its abundance in the plasma membrane of human airway epithelial cells [XREF_BIBR]."

reach
"small interference RNA mediated knockdown of USP10 increased the amount of ubiquitinated CFTR and its degradation in lysosomes, and reduced both apical membrane CFTR and CFTR mediated chloride secretion."

reach
"Moreover, a dominant negative USP10 (USP10-C424A) increased the amount of ubiquitinated CFTR and its degradation, whereas overexpression of wt-USP10 decreased the amount of ubiquitinated CFTR and increased the abundance of CFTR."
Modified USP10 decreases the amount of ubiquitinated CFTR. 2 / 2
| 2

reach
"siRNA mediated reduction of USP10 protein expression (by 76 +/-4%, XREF_FIG) increased the amount of ubiquitinated CFTR."

reach
"Moreover, a dominant negative USP10 (USP10-C424A) increased the amount of ubiquitinated CFTR and its degradation, whereas overexpression of wt-USP10 decreased the amount of ubiquitinated CFTR and increased the abundance of CFTR."
USP10 activates CFTR.
| 2
USP10 activates CFTR. 1 / 2
| 1

reach
"For example, UCH-L3 localizes to endosomes and regulates the surface levels of ENaC in kidney cells, USP-10 localizes to endosomes and promotes CFTR recycling to the epithelial cell surface, USP20 and USP33 control recycling of beta 2 ARs, and both AMSH and USP8 and UBPY are endosome associated DUBs implicated in regulating EGFR degradation."
Modified USP10 activates CFTR. 1 / 1
| 1

reach
"Moreover, a dominant negative USP10 (USP10-C424A) increased the amount of ubiquitinated CFTR and its degradation, whereas overexpression of wt-USP10 decreased the amount of ubiquitinated CFTR and increased the abundance of CFTR."
USP10 ubiquitinates CFTR.
| 2
USP10 leads to the ubiquitination of CFTR. 2 / 2
| 2

reach
"siRNA mediated knockdown of USP10 increased the multi ubiquitination and lysosomal degradation of CFTR and decreased the endocytic recycling and the half-life of CFTR in the apical membrane, as well as CFTR mediated chloride secretion."

reach
"Cif does this by stabilizing G3BP1 mediated inhibition of USP10, which then reduces USP10 mediated deubiquitination of CFTR and enhances degradation of CFTR in a lysosome [XREF_BIBR]."
USP10 inhibits CFTR.
| 1
USP10 inhibits CFTR. 1 / 2
| 1

reach
"USP10, a DUB localized at early endosomes, interacts with WT-CFTR and reduces the CFTR poly-ubiquination in CFBE cells."
CAPRIN1 affects USP10
| 8 36
| 8 19

reach
"However, it is important to note that the interactions between G3BP1 and USP10 or Caprin1 do not fully dictate SG disassembly or assembly, as two different laboratories recently demonstrated that the unstressed G3BP1 interactome significantly overlaps the interactome of G3BP1 during stress [XREF_BIBR, XREF_BIBR]."

sparser
"Subsequent work demonstrated that in addition to homo- and hetero-dimerization, G3BP1 and 2 interact with USP10 and Caprin1 [ xref , xref , xref , xref ]."

reach
"Impact of G3BP1 F33W mutation on USP10 and Caprin1 peptide binding."
| DOI

reach
"G3BP mediated SG assembly is inhibited by serine 149 phosphorylation, regulated by mutually exclusive binding of Caprin1 and USP10, and requires its RGG region that allows interactions with 40S subunits."

sparser
"G3BP1-F33W is SG competent despite its inability to bind Caprin1 or USP10, suggesting that G3BP is only regulated by Caprin1/USP10."

reach
"As a result, this disjunction between USP10 and Caprin1 binding may well reflect differences in the motif peptides, since the USP10 FGDF motif contains two phenylalanines, whereas the Caprin1 FIQDSMLD motif contains only one."
| DOI

sparser
"SG formation is rescued in these cells by expression of either wild-type G3BP, or G3BP containing a point mutation that renders it unable to bind USP10 or Caprin1."

sparser
"Nancy presented preliminary data indicating differential regulation of SG nucleation by these G3BP1 binding partners, whereby Caprin1 and USP10 bind the G3BP-NTF2-like domain in a competitive manner, with opposite results; G3BP1:Caprin1 complexes promote SG formation, whereas G3BP1:USP10 complexes inhibit SG formation."

sparser
"In addition, the acetylation-mimicking mutation K376Q impaired the RNA-dependent interaction of G3BP1 with PABP1, but its RNA-independent interactions with Caprin-1 and USP10 were little affected."

sparser
"Recently, Kedersha and colleagues reported that SG assembly is finely regulated by the binding of G3BPs to Caprin1 or USP10 [ xref ]."
| 7

sparser
"However, a recent study suggests that G3BP proteins interact with Caprin1 and USP10 but that they are mutually exclusive allowing the binding of these proteins to serve as a switch for SG formation depending on the binding partner interacting with G3BP1."

sparser
"However, it is important to note that the interactions between G3BP1 and USP10 or Caprin1 do not fully dictate SG disassembly or assembly, as two different laboratories recently demonstrated that the unstressed G3BP1 interactome significantly overlaps the interactome of G3BP1 during stress [ xref , xref ]."

sparser
"USP10 and Caprin1 antagonistically bind G3BP to regulate SG formation."

sparser
"Our results indicated that N expression abrogates G3BP1 interaction with the SG proteins CAPRIN1 and USP10 ( Figure 2C ), reinforcing the idea that N inhibits the formation of SGs in host cells by sequestering G3BPs away from their interacting SG-nucleating proteins."

sparser
"Rescue experiments using G3BP1 mutants show that phosphomimetic G3BP1-S149E fails to rescue SG formation, whereas G3BP1-F33W, a mutant unable to bind G3BP partner proteins Caprin1 or USP10, rescues SG formation."

sparser
"Therefore, although Caprin1 and USP10 both bind G3BP ( xref ; xref ), they have antagonistic effects on SGs."

sparser
"Interaction of Dengue RNA with stress granule proteins G3BP1, USP10 and caprin1 occurs at an adjacent unstructured region of the 3′ UTR ( xref )."
| 6

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [ xref ], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [ xref ]."

sparser
"However, a role for the G3BP1 and G3BP2 interactions with CAPRIN1 and/or USP10 under chronic nutrient starvation has not yet been investigated."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al.2017); SeV Trailer RNA captures TIAR from SG (Iseni et al.2002); West Nile Virus (WNV) and Dengue virus (DENV) 3′-end viral genome captures TIA-1/TIAR (Li et al.2002; Emara and Brinton 2007; Xia et al.2015); DENV 3′-UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al.2011; Reineke et al.2015); JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al.2011)."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al. 2017) ; SeV Trailer RNA captures TIAR from SG (Iseni et al. 2002) ; West Nile Virus (WNV) and Dengue virus (DENV) 3 0 -end viral genome captures TIA-1/TIAR (Li et al. 2002; Emara and Brinton 2007; Xia et al. 2015) ; DENV 3 0 -UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al. 2011; Reineke et al. 2015) ; JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al. 2011) ."

sparser
"Additional experiments demonstrate that, in contrast to DDX6, the SG proteins G3BP1, G3BP2, Caprin1 and USP10 bind to the variable region (VR) in the 3' UTR."

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [171], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [172]."
| 2

sparser
"Finally, acetylation of Lysine-376 (K376) in the RRM domain, impairs the RNA binding function of G3BP1 as well as its interaction with PABP1, USP10 or Caprin1[ xref ]."

sparser
"As previously discussed, acetylation of K376 of G3BP1 by the CBP/P300 acetylase is a key modification impeding SGs assembly by impairing its RNA binding function, as well as its interaction with PABP1, USP10 or Caprin1[ xref ]."
| 2

sparser
"The crystal structures of the nuclear transport factor 2-like (NTF2L) domain of G3BP1 in complex with the G3BP1-interacting motif (GIM) of Caprin-1 and USP10 show that both GIMs bind to the same hydrophobic pocket of G3BP1."

sparser
"These are the same domains that are necessary for the formation of SGs under conditions of cellular stress, the NTF2-like domain is needed for homodimerisation as well as binding to caprin-1 and USP10, the positive and negative regulators of SGs, while the RGG region mediates binding to the 40S ribosomal subunits [ xref ]."
SIRT6 affects USP10
1 | 24 13
SIRT6 binds USP10.
1 | 14 13
1 | 14 12

sparser
"Moreover, the specific interaction between the endogenous USP10 and SIRT6 was confirmed in HCT116 colon cancer cells because SIRT6 protein was detected in the immunoprecipitates with anti-USP10 antibody, but not with the control mouse IgG ( xref )."

reach
"We demonstrated the interaction between USP10 and Sirt6 in AB induced CH phenotype by USP10 knockout or transgenic mice, but no Sirt6 knockout or transgenic mice."

reach
"As indicated in XREF_FIG, USP10 interacts with SIRT6 but not with any of the other six sirtuin family proteins."

reach
"For example, in colon cancer, USP10 interacted with and stabilized SIRT6 through suppressing SIRT6 ubiquitination [25]."

sparser
"USP10 interacts with SIRT6."

reach
"For example, in colon cancer, USP10 interacted with and stabilized SIRT6 through inhibiting SIRT6’s ubiquitination, suppressing proliferation [25]."

sparser
"Collectively, these data demonstrate that USP10 can interact with Sirt6 and promote the ubiquitination of Sirt6 in cardiomyocytes and then inhibit the downstream Akt signaling pathway and cardiac hypertrophy."

sparser
"We demonstrated the interaction between USP10 and Sirt6 in AB‐induced CH phenotype by USP10 knockout or transgenic mice, but no Sirt6 knockout or transgenic mice."

No evidence text available

sparser
"As indicated in xref , USP10 interacts with SIRT6 but not with any of the other six sirtuin family proteins."
| 1

sparser
"Furthermore, USP10 physically binds to sirtuin 6 (Sirt6) and Sirt6 mediates the effect of USP10 on the regulation of CH."
SIRT6 activates USP10.
| 8
SIRT6 activates USP10. 8 / 8
| 8

reach
"32 These findings present evidence that Sirt6 mediates the effect of USP10 in the regulation of CH in response to Ang II stimulation."

reach
"Ang II induced cardiac hypertrophy potentiated by USP10 knockdown was also completely blocked by Sirt6 activity upregulation."

reach
"Our study has presented evidence that Sirt6 mediates the effect of USP10 in the regulation of CH in response to Ang II stimulation."

reach
"These findings present evidence that Sirt6 mediates the effect of USP10 in the regulation of CH in response to Ang II stimulation."

reach
"These findings demonstrated that the Sirt6 mediated the effect of USP10 in regulation of CH in response to Ang II stimulation."

reach
"To further prove the principle that USP10 suppresses c-Myc transcriptional activity through both SIRT6 and p53, we tested whether knockdown of SIRT6 rescues this USP10 suppressive activity."

reach
"Sirt6 Mediates USP10 Regulated Cardiac Hypertrophy."

reach
"Furthermore, USP10 physically binds to sirtuin 6 (Sirt6) and Sirt6 mediates the effect of USP10 on the regulation of CH."
SIRT6 inhibits USP10.
| 2
SIRT6 inhibits USP10. 2 / 2
| 2

reach
"Limonin mediates the ubiquitination and degradation of SIRT6 by activating USP10, providing an attractive therapeutic target for cardiac hypertrophy."

reach
"Indeed, knockdown of SIRT6 in p53 +/+ cells partially reversed the suppressive activity of USP10."

reach
"Proliferation inhibition by loss of USP10 depends on SKP2 functional status."

reach
"HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway."

reach
"Interesting, Spautin‐1 imposed minimal effect on the cell proliferation (Fig. S5C), suggesting that under our experimental conditions, USP10 mainly modulated cell migration rather than cell proliferation, through the regulatory effect on Smad4."

reach
"USP10 regulates Musashi-2 stability via deubiquitination and promotes tumour proliferation in colon cancer."

reach
"We found that loss of USP10 consequently suppressed the proliferation of CML cells and induced G0/G1 phase arrest in all CML cell lines examined, suggesting that USP10 is critical to CML cell proliferation."

reach
"Targeting USP10 by shRNA inhibited CRC proliferation and metastasis, and this effect was reversed by overexpression of NLRP7."

reach
"As shown in XREF_FIG, downregulation of USP10 increased cancer cell proliferation in p53 +/+ cells, while USP10 expression levels have no apparent effect on proliferation in cells lacking p53."

reach
"Moreover, inhibition of USP10 significantly suppresses the proliferation of both imatinib sensitive and imatinib resistant CML cells, which likely depends on SKP2 status."

reach
"Paradoxically, USP10 could promote cancer cell proliferation in mutant p53 background."

reach
"Moreover, we have also experimentally demonstrated that both pharmacological and genetic inhibition of USP10 are capable of, in a SKP2-depednet manner, suppressing the proliferation of both IM sensitive and IM resistant CML cells in cell cultures and in mouse xenograft models."

reach
"Furthermore, the double knock-down of G3BP1 and USP10 rescued the inhibition of proliferation induced by G3BP1 depletion in HCT116 (XREF_FIG) and SK-MEL-5 cells (XREF_SUPPLEMENTARY)."

reach
"Furthermore, we provide evidence that USP10 inhibits cancer cell proliferation in cells with WT p53."

reach
"Consistent with its molecular activity, re-expression of USP10 suppressed NSCLC cell proliferation and migration while knockout of USP10 promoted NSCLC cell proliferation and migration."

reach
"On the other hand, reconstitution of USP10 in cells with USP10 downregulation inhibited cancer cell proliferation (XREF_FIG), suggesting the effect of USP10 knockdown is specific."

reach
"In contrast, overexpressing USP10 expression in MHCC97L and BEL-7402 cells reduced the cell proliferation."

reach
"The research by Yuan et al. demonstrated that USP10 inhibits cancer cell proliferation in wild-type p53 cells, but promotes tumorigenesis in a mutant p53 background (Yuan et al., 2010)."

reach
"We showed that loss or inhibition of USP10 not only suppressed the proliferation of imatinib sensitive and imatinib resistant cells in vitro but also inhibited the growth of imatinib sensitive and imatinib resistant xenografts in vivo."

reach
"Next, we treated infected THP-1 cells with a cell-permeable potent autophagy inhibitor, spautin-1.58 Spautin-1 promotes the degradation of BECN1 by inhibiting 2 USPs (ubiquitin-specific peptidases), USP10 and USP13, which target BECN1.58 Spautin-1 treatment of infected cells significantly decreased E. chaffeensis proliferation (Fig. 3B)."

reach
"Conversely, downregulation of USP10 inhibited cell proliferation (XREF_FIG and data not shown)."

reach
"As expected, USP10 over expression inhibited the proliferation of colon cancer cells with either a wildtype p53 or with p53-deficency."
USP10 affects SQSTM1
| 13 22
USP10 binds SQSTM1.
| 5 20
| 5 18

sparser
"Moreover, research shows that USP10 interacts with p62 and augments p62-dependent ubiquitinated protein aggregation and aggresome formation, which inhibit proteasome activity [ xref ]."

sparser
"These ubiquitinated proteins bind to p62 and USP10, and the formed aggregates inhibit proteasome activity to further increase the amount of ubiquitinated proteins."

sparser
"These results suggested that protein–protein interaction between p62 and USP10 is not necessary for the formation of p62-bodies in dopamine-treated cells."

sparser
"These results suggested that USP10 interacts with p62 to increase the amount of pp62-S349 and that pp62-S349 increased by USP10 reduces the degradation of Nrf2 in dopamine-treated cells."

reach
"To promote protein aggregation and aggresome formation, USP10 interacted with p62, and they cooperatively inhibited caspase-3-associated cell death."

reach
"These results suggested that multiple interactions between USP10 and p62 are required for maximum aggresome promoting activity of USP10."

sparser
"To promote protein aggregation and aggresome formation, USP10 interacted with p62, and they cooperatively inhibited caspase-3-associated cell death."

reach
"Thus, we examined whether USP10 interacts with p62."

sparser
"The immunoprecipitation assay indicated that endogenous p62 interacted with endogenous USP10 together with previously known p62 interactors HDAC6 or dynein ( xref , xref ), both of which are important for aggresome formation ( xref A)."

sparser
"Interaction of USP10 with p62 promoted protein aggregation and aggresome formation ( xref )."
| 2

sparser
"G3BP1 interacts with p62 and USP10 to inhibit protein aggregation."

sparser
"G3BP1 interacted with p62 and USP10 and inhibited p62/USP10-induced protein aggregation."
USP10 activates SQSTM1.
| 4
USP10 activates SQSTM1. 4 / 4
| 4

reach
"In addition to USP10-WT, USP10 96-798, but not USP10 1-116, increased the size of p62 aggregates and aggresomes (XREF_FIG E and 5F)."

reach
"USP10, by interacting with p62, further augments p62-induced ubiquitinated protein aggregation and then stimulates the transport of p62/ubiquitinated protein aggregates to the perinuclear aggresome-forming site."

reach
"In addition, USP10 stimulates the transport of multiple p62-positive ubiquitinated protein aggregates to the perinuclear region to form one big aggregate (aggresome)."

reach
"These results suggested that USP10 functions to stimulate transport of p62 aggregates to the perinuclear aggresome formation site."
USP10 phosphorylates SQSTM1.
| 1 1
USP10 leads to the phosphorylation of SQSTM1. 2 / 2
| 1 1

sparser
"The increase in p62 phosphorylation by USP10 (pp62-S349) can be explained by several mechanisms."

reach
"USP10 interacted with the Nrf2 activator p62, increased the phosphorylation of p62, increased the interaction of p62 with the Nrf2 inhibitor Keap1, and stimulated Nrf2 antioxidant transcriptional activity."
USP10 inhibits SQSTM1.
| 1 1
USP10 inhibits SQSTM1. 2 / 2
| 1 1

sparser
"For instance, USP10 may stimulate p62 kinase or inhibit p62-phosphatase."

reach
"These results indicated that USP10 and p62 cooperatively inhibit MG-132-induced cell death by promoting the formation of aggresomes and p62 aggregates."
USP10 increases the amount of SQSTM1.
| 2
Modified USP10 increases the amount of SQSTM1. 2 / 2
| 2

reach
"Moreover, USP10 expression increased the amount of insoluble p62 in p62 wild-type cells (XREF_FIG B)."

reach
"USP10 overexpression without MG-132 treatment increased the amounts of p62 and insoluble ubiquitinated proteins (XREF_FIG B), whereas USP10-KD cells treated with MG-132 increased the amounts of p62 and ubiquitinated proteins more than USP10-WT cells (XREF_FIG E)."
| 11 23
USP10 translocates to the nucleus. 10 / 31
| 9 22

sparser
"Alternatively, USP10 could translocate into the nucleus to participate in DNA damage response."

sparser
"After DNA damage, USP10 is stabilised, and a fraction of USP10 translocates to the nucleus to activate p53."

sparser
"USP10 is upregulated and translocates to the nucleus following DNA damage and regulates p53-dependent DNA damage response."

sparser
"Interestingly USP10, a predominantly cytoplasmic DUB, is involved in homeostasis of cytoplasmic p53 in unstressed cells, but, following DNA damage, a fraction of USP10 can translocate into the nucleus where it contributes to p53 activation [ xref ]."

sparser
"Upon DNA damage, USP10 can translocate to the nucleus and also contribute to p53 activation xref xref ."

sparser
"Following DNA damage, USP10 also translocates to the nucleus and contributes to p53 activation."

sparser
"For example, USP10, a cytoplasmic DUB, translocates to the nucleus upon DNA damage where it contributes to the stabilization of p53 [ xref ]."

reach
"This process is very important during DNA-damage response, in which USP10 translocates to the nucleus and deubiquitinates p53, stabilizing it and thus regulating its response to DNA damage [113,114,115]."

sparser
"Following DNA damage, phosphorylation of USP10 by ATM leads to translocation of USP10 into the nucleus, where USP10 deubiquitinates and stabilizes nuclear p53 [ xref ]."

sparser
"Upon induction by DNA damage, a fraction of USP10 is phosphorylated by ATM and translocates to the nucleus where it deubiquitinates p53."
USP10 translocates from the cytoplasm to the nucleus. 3 / 3
| 2 1

sparser
"In response to cell stress, USP10 was translocated from the cytoplasm to the nucleus to regulate cell cycle and apoptosis ( xref ; xref )."

reach
"In response to cell stress, USP10 was translocated from the cytoplasm to the nucleus to regulate cell cycle and apoptosis (Lim et al., 2019; Zhang et al., 2020)."

reach
"12 Under normal conditions, USP10 is localized to the cytoplasm; however, USP10 translocates from the cytoplasm to the nucleus in response to cell stress and subsequently influences p53 localization."
USP10 affects AR
2 | 15 8
USP10 binds AR.
2 | 4 8
2 | 4 8

No evidence text available

sparser
"In order to determine whether the interaction of USP10 with the AR affected its transcriptional function, a construct for transient expression was devised in the pSG5 plasmid."

sparser
"Moreover, USP10 not only binds AR, resulting in increased transcriptional activity, but also deubiquitinates the histone variant H2A.Z, both of which are required for AR − mediated gene activation [ xref , xref ]."

reach
"USP10 was originally detected in DNA bound AR complexes but the results of GST pull-down experiments indicate that USP10 may interact with the AR also in absence of response elements, at least when us[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

No evidence text available

sparser
"USP10 was originally detected in DNA-bound AR complexes but the results of GST pull-down experiments indicate that USP10 may interact with the AR also in absence of response elements, at least when us[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The difference in migration was probably due to post-translational modifications.In order to further analyse the interaction between USP10 and the AR, GST pull-down experiments were performed."

sparser
"In order to further analyse the interaction between USP10 and the AR, GST pull-down experiments were performed."

reach
"Usp10 can bind to AR and positively regulate its transcriptional activity [XREF_BIBR] however, it also regulates AR indirectly via deubiquitination of H2A.Z causing the same effect [XREF_BIBR]."

sparser
"Our data suggest that if USP10 and AR do physically interact, this interaction only occurs in a small nuclear fraction of USP10."
USP10 activates AR.
| 10
USP10 activates AR. 10 / 12
| 10

reach
"For example, USP10 stimulates AR transcriptional activity in the presence of its synthetic agonist R1881 [67]."

reach
"USP10 overexpression enhanced AR transcriptional activity whereas knock-down by specific siRNAs had the opposite effect."

reach
"USP10 appeared to modulate AR function."

reach
"Cell based transactivation assays in PC-3/AR cells revealed that overexpression of wild-type USP10, but not of an enzymatically inactive form, stimulated AR activity mediated by reporter constructs harbouring selective androgen response elements (AREs), non selective steroid response elements (SREs) or the mouse mammary tumour virus (MMTV) promoter."

reach
"The coactivating effects were observed with selective and non selective response elements, indicating that USP10 was a broad coactivator of the AR."

reach
"Usp10 is known to enhance AR transcriptional activity [XREF_BIBR, XREF_BIBR] and Usp22 and Usp26 have been found in complexes with AR [XREF_BIBR, XREF_BIBR]."

reach
"Overexpression of wild-type USP10 stimulated AR activity as revealed by reporter constructs harbouring selective androgen response elements, non selective steroid response elements or the mouse mammary tumour virus promoter."

reach
", confirming that USP10 is a bonafide co-activator of AR regulated transcription."

reach
"Usp10, another DUB reported to target AR, was also shown to enhance AR activity by combining both direct and indirect activity."

reach
"RNF6 and USP10, which modulate androgen receptor function, showed correlated genomic loss and lower expression in a subset of tumors and gain and higher expression in others."
USP10 inhibits AR.
| 1
USP10 inhibits AR. 1 / 3
| 1

reach
"In contrast, the siRNA luciferase screen in LNCaP cells showed that transfection with validated siRNAs targeting USP10 potentiated AR transcriptional activity."
USP10 affects HDAC6
| 1 15 16
USP10 binds HDAC6.
| 6 16
| 6 16

sparser
"To determine whether USP10 binds to HDAC6 directly or via other proteins, we generated and purified recombinant GST-USP10 and His-HDAC6 in E. coli ."

sparser
"Implications of the USP10-HDAC6 axis in lung cancer - A path to precision medicine."

sparser
"Finally, as the current landscape for the treatment of advanced lung cancer has shifted towards anti-PD-l/PD-L1-based immunotherapy [ xref ], further investigations to connect the USP10-HDAC6 axis to immunotherapy are certainly warranted."

sparser
"Overall, our data suggest targeting the USP10-HDAC6 axis in NSCLC lacking wild-type p53 (Fig. xref )."

sparser
"USP10 domain mapping found a more restricted binding pattern, as besides the full-length, the very C-terminal region (amino acids 700–798) was the only one necessary for the interaction between USP10 and HDAC6 to occur (Fig. xref )."

reach
"In the null-p53 or mutant-p53 background, USP10 may largely bind to HDAC6."

reach
"USP10 interacts with HDAC6."

reach
"Next, we mapped which domain (s) of HDAC6 binds to USP10, and vice versa."

sparser
"Our investigations then revealed that HDAC6 interacts with USP10, and it is a substrate of USP10."

reach
"To determine whether USP10 binds to HDAC6 directly or via other proteins, we generated and purified recombinant GST-USP10 and His HDAC6 in E. coli."
USP10 increases the amount of HDAC6.
| 4
USP10 increases the amount of HDAC6. 4 / 4
| 4

reach
"XREF_FIG (lanes 1-7), only wild-type USP10, but not the catalytically deficient mutant (USP10 and C424A), significantly increased the endogenous level of HDAC6, indicating that USP10 's deubiquitinase activity is imperative for increasing the protein level of HDAC6."

reach
"Depletion or inhibition of USP10 significantly decreases HDAC6 protein levels, inhibits tumor growth, and increases p53-mutant and null cancer cell sensitivity to cisplatin in a xenograft mouse model."

reach
"XREF_FIG, knockdown of USP10 decreased HDAC6 protein levels as compared to the control H23 cells, while MG132 restored HDAC6 expression in the knockdown cells, indicating that blocking proteasome degradation leads to HDAC6 accumulation."

reach
"The above results suggest that USP10 up-regulates HDAC6 protein expression, most likely through deubiquitinating and consequently stabilizing HDAC6."
USP10 deubiquitinates HDAC6.
| 3
USP10 deubiquitinates HDAC6. 3 / 3
| 3

reach
"Next, we tested whether USP10 could deubiquitinate HDAC6."

reach
"Another previous study from Hu et al. also showed that the depletion of USP10 increased the K48-linked polyubiquitination of HDAC6 in the non-small-cell lung cancer (NSCLC) cell line H1299."

reach
"XREF_FIG, wild-type USP10, but not the catalytically-inactive USP10CA mutant, significantly reduces HDAC6 ubiquitination in 293T cells (comparing lanes 3 and 4 in the top panel)."
USP10 activates HDAC6.
| 1 2
USP10 activates HDAC6. 3 / 3
| 1 2

eidos
"We first hypothesize that in a wild-type p53 background , depletion of USP10 leads to the reduction of HDAC6 as well as affecting an unknown factor , which counteracts the effect of HDAC6 reduction ."

reach
"Overall, our data suggest that USP10 increases HDAC6 protein stability."

reach
"Collectively, these results suggest that USP10 stimulates HDAC6 mediated aggresome inducing activity."
USP10 affects PTEN
4 1 | 2 17 5
USP10 binds PTEN.
4 | 7 4
4 | 7 1

reach
"Therefore, we conclude that USP10 interacts with PTEN and AMPKalpha, inhibits their polyubiquitylation, and as a result stabilizes PTEN and AMPKalpha.Next, we examined whether Rapamycin, a well known [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

No evidence text available

reach
"Inversely, endogenous PTEN and AMPKalpha interacted with endogenous USP10 in HCC-LM3 and HUH7 cells."

No evidence text available

No evidence text available

reach
"Mechanistically, USP10 interacts and stabilizes PTEN and AMPKalpha by inhibiting their polyubiquitylation."

reach
"We further demonstrated that USP10 directly interacted with and stabilized PTEN via deubiquitination."

reach
"Furthermore, USP10 can also interact with and deubiquitinate PTEN, USP10 inhibition stimulates tumor growth and invasion, but this effect can be abolished by reinserting PTEN (Sun et al., 2018)."

sparser
"We found using co-immununoprecipitation/immunoblotting that USP10 interacted with PTEN and reduced the K63-linked polyubiquitination of PTEN mediated by TRIM25 in non-small cell lung cancer (NSCLC) cells."

reach
"Mechanistically, USP10 interacts with and stabilizes PTEN and AMPKalpha by inhibiting their polyubiquitylation, and subsequently suppresses the activity of mTOR signaling pathway."
| 3

sparser
"Therefore, we conclude that USP10 interacts with PTEN and AMPKα, inhibits their polyubiquitylation, and as a result stabilizes PTEN and AMPKα."

sparser
"To further dissect how USP10 inhibits mTOR activity, we investigated whether the stabilization of PTEN [] and AMPKα [] by USP10 involved the physical interactions of USP10 with PTEN and AMPKα."

sparser
"Inversely, endogenous PTEN and AMPKα interacted with endogenous USP10 in HCC-LM3 and HUH7 cells (B)."
USP10 deubiquitinates PTEN.
1 | 4
USP10 deubiquitinates PTEN. 5 / 5
1 | 4

"We further demonstrated that USP10 directly interacted with and stabilized PTEN via deubiquitination."

reach
"In addition, in line with our study, two different groups have also reported that USP10 deubiquitinates and stabilizes PTEN and AMPKalpha in lung cancer and colon cancer cells [16, 27]."

reach
"Additionally, we found that USP10 stabilized PTEN protein in a dose dependent manner, and inhibited Lys-48-linked polyubiquitylation of PTEN."

reach
"Furthermore, USP10 can also interact with and deubiquitinate PTEN, USP10 inhibition stimulates tumor growth and invasion, but this effect can be abolished by reinserting PTEN (Sun et al., 2018)."

reach
"In this study, we identified that USP10 can deubiquitinate PTEN and AMPKalpha and the stabilization of these two proteins will subsequently suppress the activation of AKT and mTOR in HCC cells."
USP10 activates PTEN.
| 1 3 1
USP10 activates PTEN. 5 / 5
| 1 3 1

sparser
"Specifically, USP10 activates PTEN by preventing its K63-linked polyubiquitination mediated by TRIM25 and suppresses the AKT/mTOR signaling pathway thereby inhibiting NSCLC proliferation, indicating that it may be a potential drug target for cancer treatment."

reach
"Specifically, USP10 activates PTEN by preventing its K63 linked polyubiquitination mediated by TRIM25 and suppresses the AKT and mTOR signaling pathway thereby inhibiting NSCLC proliferation, indicating that it may be a potential drug target for cancer treatment."

eidos
"Specifically , USP10 activates PTEN by preventing its K63-linked polyubiquitination mediated by TRIM25 and suppresses the AKT / mTOR signaling pathway thereby inhibiting NSCLC proliferation , indicating that it may be a potential drug target for cancer treatment ."

reach
"The deubiquitinase USP10 restores PTEN activity and inhibits non small cell lung cancer cell proliferation."

reach
"In contrast to TRIM25, USP10 restored PTEN phosphatase activity and reduced the production of the secondary messenger PI (3,4,5) P3, thereby inhibiting AKT and mTOR pro growth signaling transduction in NSCLC cells."
USP10 inhibits PTEN.
| 1 3
USP10 inhibits PTEN. 4 / 4
| 1 3

reach
"USP10 suppresses lung cancer cell invasion and growth via upregulating PTEN [22]."

reach
"USP10 was reported to inhibit tumor growth and inactivate mTORC1 and AKT signaling by stabilizing AMPKalpha and PTEN in HCC cells."

eidos
"Moreover , USP10 , but not its inactive C424A deubiquitinating mutant or other deubiquitinases , abolished PTEN from K63-linked polyubiquitination mediated by TRIM25 ."

reach
"Moreover, USP10, but not its inactive C424A deubiquitinating mutant or other deubiquitinases, abolished PTEN from K63 linked polyubiquitination mediated by TRIM25."
USP10 affects G3BP2
5 | 7 13
USP10 binds G3BP2.
5 | 2 13
5 | 2 3

sparser
"For example, USP10 interacts with G3BP2 to block p53 signal transduction, leading to poor prognosis in prostate cancer [ xref ]; USP44 promotes the development of prostate cancer by stabilizing EZH2 [ xref ], and USP2a enhances c-Myc expression via microRNA-related regulation and thus promotes tumourigenesis [ xref ]."

sparser
"The association of USP10 with G3BP2 suppresses p53 signalling which correlates to a poor prognosis in prostate cancer, highlighting an oncogenic role of USP10 in prostate cancer via G3BP2 [ xref ]."

No evidence text available

No evidence text available

reach
"For example, USP10 interacts with G3BP2 to block p53 signal transduction, leading to poor prognosis in prostate cancer [XREF_BIBR]; USP44 promotes the development of prostate cancer by stabilizing EZH2 [XREF_BIBR], and USP2a enhances c-Myc expression via microRNA related regulation and thus promotes tumourigenesis [XREF_BIBR]."

No evidence text available

sparser
"The N protein compromised G3BP1-USP10 and 345 G3BP1-PKR interactions (Fig. 4b, c) and disrupted G3BP2-USP10 and G3BP2-PKR interactions 346 (Fig. 4d, e) ."

reach
"Furthermore, USP10, a binding partner of G3BP2, is also an androgen responsive gene and is transcriptionally activated by the AR."

No evidence text available

No evidence text available
| 6

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [ xref ], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [ xref ]."

sparser
"However, a role for the G3BP1 and G3BP2 interactions with CAPRIN1 and/or USP10 under chronic nutrient starvation has not yet been investigated."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al.2017); SeV Trailer RNA captures TIAR from SG (Iseni et al.2002); West Nile Virus (WNV) and Dengue virus (DENV) 3′-end viral genome captures TIA-1/TIAR (Li et al.2002; Emara and Brinton 2007; Xia et al.2015); DENV 3′-UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al.2011; Reineke et al.2015); JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al.2011)."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al. 2017) ; SeV Trailer RNA captures TIAR from SG (Iseni et al. 2002) ; West Nile Virus (WNV) and Dengue virus (DENV) 3 0 -end viral genome captures TIA-1/TIAR (Li et al. 2002; Emara and Brinton 2007; Xia et al. 2015) ; DENV 3 0 -UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al. 2011; Reineke et al. 2015) ; JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al. 2011) ."

sparser
"Additional experiments demonstrate that, in contrast to DDX6, the SG proteins G3BP1, G3BP2, Caprin1 and USP10 bind to the variable region (VR) in the 3' UTR."

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [171], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [172]."
| 4

sparser
"Validation of the mass spectrometry data confirmed the interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."

sparser
"NCAP showed specific interaction with the SG proteins G3BP1, G3BP2, YTHDF3, USP10 and PKR, and translocated to SGs following oxidative stress and heat shock."

sparser
"NCAP interacts with SG proteins. (A) Interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."

sparser
"Fig. 4: NCAP interacts with SG proteins. (A) Interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."
USP10 inhibits G3BP2.
| 1
USP10 inhibits G3BP2. 1 / 3
| 1

reach
"GFP-USP10 overexpression inhibits B-isox precipitation of G3BP2 (XREF_FIG, lanes 4-6 vs. 1-3) and modestly decreases that of G3BP1 and Caprin1, whereas that of TIAR is unchanged."
USP10 decreases the amount of G3BP2.
| 2
USP10 decreases the amount of G3BP2. 2 / 2
| 2

reach
"USP10 regulates androgen mediated signalling and inhibits p53 activities by regulating G3BP2 expression."

reach
"However, USP10 KD also reduces expression of G3BP1 and G3BP2 (to 58% and 77% of control values, respectively; XREF_FIG), suggesting that the SG inhibition caused by USP10 KD may be caused by reduced levels of G3BP."
USP10 activates G3BP2.
| 1
USP10 activates G3BP2. 1 / 2
| 1

reach
"It should be noted that the G3BP1-KD-induced G3BP2 increase was also attenuated by p62-KD or USP10-KD."
USP10 increases the amount of G3BP2.
| 1
Modified USP10 increases the amount of G3BP2. 1 / 1
| 1

reach
"Expression of GFP-USP10 (lanes 4-6 and 16-18) increases G3BP2 expression (XREF_FIG, lanes 10-12 vs. 7-9), but the increased G3BP2 is not precipitated by B-isox (lanes 4-6), suggesting that B-isox binds and/or precipitates a SG-competent form of G3BP that is rendered SG-incompetent and B-isox soluble upon USP10 binding."
USP10 affects Circulin-C
| 28

sparser
"Furthermore, a subnetwork regulating microglial/macrophage activation that contained circ_0014637 (circ-Usp10), 5 miRNAs (miR-149, miR-152, miR-7033, miR-709 and miR-6963) and 8 target genes (Sbno2, Thbs1Cx3cr1, Cd300a, Il4ra, Syk, Ctsc, Cd84, and Sbno2) was further screened ( xref ))."

sparser
"qRT–PCR, ELISA, flow cytometry, and luciferase reporter assays were carried out to validate the role of circ_0014637 (circ-Usp10) and microRNA(miR)-152-5p /CD84 in microglia."

sparser
"A concurrent decrease in circ-Usp10 expression occurred with elevated expression of miR-152 and a decrease in CD84 in BV2 cells."

sparser
"Nevertheless, the function of circ-Usp10 in SCI is still poorly understood."

sparser
"These results revealed that circ-Usp10 promoted microglial activation and induced neuronal death by targeting miR-152-5p/CD84, which might provide a new potential therapy for SCI."

sparser
"We found that circ-Usp10 inhibited the secretion of proinflammatory factors in microglial BV2 cells."

sparser
"Three siRNAs were designed to influence the expression of circ-Usp10 in microglial BV2 cells stimulated by LPS."

sparser
"The circ-Usp10 may be a diagnostic biomarker and potential target for SCI therapy."

sparser
"To investigate and verify the function of circ-Usp10 in SCI in vitro, we performed a cellular experiment in vitro."

sparser
"Nevertheless, the effect of circ-Usp10 on SCI has not yet been reported."
USP10 affects PCNA
1 | 14 12
USP10 binds PCNA.
1 | 9 12
1 | 9 12

reach
"These results indicate that USP10 preferentially binds to ISGylated PCNA to cleave off mono-ubiquitin for TLS termination.Significantly, USP10 interacted with PCNA when doubly ISGylated PCNA appeared [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Significantly, USP10 interacted with PCNA when doubly ISGylated PCNA appeared (i.e., at 24 hr after UV treatment), but not when PCNA was mono-ISGylated (i.e., at 12 hr) ( Figure 3 J)."

sparser
"Figure 3 H shows that USP10 binds to ISGylated PCNA much more tightly than to its unmodified form."

sparser
"These results indicate that USP10 preferentially binds to ISGylated PCNA to cleave off mono-ubiquitin for TLS termination."

reach
"Moreover, PCNA binding to USP10 was abrogated by K168R mutation or ISG15 knockdown, both of which block PCNA ISGylation."

reach
"This important question awaits further studies.Surprisingly, the binding of USP10 to unmodified endogenous PCNA could not be detected, despite the fact that it has a noncanonical PIP box."

sparser
"ISGylated PCNA promotes the interaction between PCNA and USP10 and de-ubiquitination of mono-ubiquitinated PCNA."

reach
"Ubiquitylated PCNA promotes its ISGylation 12 h after UV irradiation [228] and the ISGylated PCNA binds to USP10, which then removes ubiquitin from PCNA to stop TLS [228]."

reach
"Likewise, the binding of USP10 to PCNA via its noncanonical PIP box might be very weak, but it could be markedly enhanced by PCNA ISGylation, and this enhancement might provide the ability of USP10 to[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Likewise, the binding of USP10 to PCNA via its noncanonical PIP box might be very weak, but it could be markedly enhanced by PCNA ISGylation, and this enhancement might provide the ability of USP10 to[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP10 deubiquitinates PCNA.
| 5
USP10 deubiquitinates PCNA. 5 / 5
| 5

reach
"Moreover, USP10 could induce PCNA deubiquitination to promote HCC cell growth."

reach
"Recent research has shown that PCNA is also modified by ISG15, and ISGylation of PCNA recruits USP10 to deubiquitinate PCNA, thereby regulating PCNA ubiquitination under UV irradiation induced stress."

reach
"And USP10 could strengthen cell proliferative and migratory abilities through deubiquitinating PCNA."

reach
"An interesting study has recently shown that the deubiquitylation of mammalian PCNA, at least in the context of DNA damage induced by ultraviolet irradiation (UV), is aided by the deubiquitylating activity of USP10."

reach
"This modification in turn recruits USP10 that de-ubiquitylates PCNA in order to block TLS and resume normal replication."
SQSTM1 affects USP10
| 5 20
| 5 18

sparser
"Moreover, research shows that USP10 interacts with p62 and augments p62-dependent ubiquitinated protein aggregation and aggresome formation, which inhibit proteasome activity [ xref ]."

sparser
"These ubiquitinated proteins bind to p62 and USP10, and the formed aggregates inhibit proteasome activity to further increase the amount of ubiquitinated proteins."

sparser
"These results suggested that protein–protein interaction between p62 and USP10 is not necessary for the formation of p62-bodies in dopamine-treated cells."

sparser
"These results suggested that USP10 interacts with p62 to increase the amount of pp62-S349 and that pp62-S349 increased by USP10 reduces the degradation of Nrf2 in dopamine-treated cells."

reach
"To promote protein aggregation and aggresome formation, USP10 interacted with p62, and they cooperatively inhibited caspase-3-associated cell death."

reach
"These results suggested that multiple interactions between USP10 and p62 are required for maximum aggresome promoting activity of USP10."

sparser
"To promote protein aggregation and aggresome formation, USP10 interacted with p62, and they cooperatively inhibited caspase-3-associated cell death."

reach
"Thus, we examined whether USP10 interacts with p62."

sparser
"The immunoprecipitation assay indicated that endogenous p62 interacted with endogenous USP10 together with previously known p62 interactors HDAC6 or dynein ( xref , xref ), both of which are important for aggresome formation ( xref A)."

sparser
"Interaction of USP10 with p62 promoted protein aggregation and aggresome formation ( xref )."
| 2

sparser
"G3BP1 interacts with p62 and USP10 to inhibit protein aggregation."

sparser
"G3BP1 interacted with p62 and USP10 and inhibited p62/USP10-induced protein aggregation."
USP10 affects MYC
| 20
USP10 inhibits MYC.
| 18
USP10 inhibits MYC. 10 / 22
| 18

reach
"It has been reported that one of the molecular mechanisms underlying USP10 tumor-suppressive functions is that it protects p53 from degradation, raising the possibility that USP10 also antagonizes c-Myc transcriptional activity through p53."

reach
"In addition, p53 knockdown partially abolished USP10 over-expression-mediated suppression in c-Myc target gene expression in SIRT6 +/+ MEFs, and p53 knockdown in SIRT6 -/- MEFs fully reversed USP10 mediated suppression of c-Myc targets expression (XREF_FIG)."

reach
"To further prove the principle that USP10 suppresses c-Myc transcriptional activity through both SIRT6 and p53, we tested whether knockdown of SIRT6 rescues this USP10 suppressive activity."

reach
"We then used SIRT6-null mouse embryonic fibroblasts (MEFs) to prove the principle that SIRT6 is another downstream factor in USP10 mediated c-Myc suppression."

reach
"USP10 inhibits c-Myc transcriptional activity."

reach
"These results indicate that p53 is downstream of USP10 in suppressing c-Myc transcriptional activity, but additional factors, one of which presumably is SIRT6 as indicated from our results in XREF_FIG, are involved in USP10 mediated c-Myc suppression."

reach
"In human colon cancer, USP10 and SIRT6 protein expressions were reduced, and USP10 antagonized c-Myc transcriptional activity through SIRT6 and p53 to inhibit cell cycle progression, cancer cell growth, and tumor formation [XREF_BIBR]."

reach
"Third, USP10 antagonizes c-Myc transcriptional activity and cell cycle progression through both SIRT6 and p53."

reach
"Previous studies have shown that USP10 antagonizes c-Myc transcriptional activation that could promote Sirt6 stabilization to suppress tumor formation."

reach
"USP10 suppresses c-Myc transcriptional activity through SIRT6 and p53."
USP10 activates MYC.
| 2
USP10 activates MYC. 2 / 2
| 2

reach
"To our surprise, SIRT6 gene suppression resulted in a lower level of increase in c-Myc target gene expression than that of USP10 knockdown, implying that additional factors may contribute to USP10 mediated c-Myc suppression."

reach
"Thus, we determined whether USP10 functions as a tumor suppressor through SIRT6 mediated c-Myc inactivation."
USP10 affects BECN1
1 | 19 4
USP10 deubiquitinates BECN1.
1 | 11
USP10 deubiquitinates BECN1. 10 / 12
1 | 11

reach
"It promotes the degradation of Vps34 complexes by inhibiting USP10 and USP13, two ubiquitin specific peptidases that target the deubiquitination of Beclin-1."

reach
"Since USP10 mediates the deubiquitination of Beclin1 and reduced levels of USP10 leads to increased ubiquitination and degradation of Vps34 complexes, reduced levels of Vps34 complexes as a result of USP10 reduction may in turn lead to destabilization of USP13."

reach
"On the other hand, autophagy can be also inhibited blocking PI3KC3 complex formation; Spautin-1 indirectly inhibits the activity of VPS34 by proteosomal degradation of proteins that form VPS34 complexes through reduction of Beclin-1 deubiquitination mediated by USP10 and USP13 [120]."

reach
"USP10 has also been shown to enhance autophagy by deubiquitinating beclin-1, a component of class-III PI3K complexes that catalyze the formation of phosphatidylinositol 3-phosphate at both autophagy initiation and autophagosome maturation steps."

reach
"Inhibition of the Deubiquitinating Enzymes USP10 and USP13 Causes Ubiquitination of Beclin 1."

reach
"Conversely, it was show that Beclin 1 is deubiquitinated by USP10 and USP13 and adding complexity, Beclin 1 itself controlled the protein stabilities of USP10 and USP13 by regulating their deubiquitinating activities, in turn regulating the levels of tumor suppressor p53 [XREF_BIBR]."

reach
"Furthermore, the presence of spautin-1 inhibited the deubiquitination of Beclin1 mediated by USP10 and USP13."

reach
"Specific and potent autophagy inhibitor-1 (Spautin-1) was identified to inhibit USP10 and USP13, which deubiquitinate the Beclin 1 subunit of Vsp34 complex, and thus promoted the degradation of Vsp34 PI3 kinase complex."

"While A20 inhibits PtdIns3P signaling by removing the TRAF6-dependent Lys63-linked chains from Beclin 1, the enzymes USP10 and USP13 prevent PI3K-III complex components from their degradation and, therefore, support autophagy. Interestingly enough, USP10 also stabilizes p53, which, in turn, triggers the degradation of Beclin 1 and VPS34 in order to prevent autophagy."

reach
"We demonstrate that USP10 and USP13 can both mediate the deubiquitination of Beclin1."
USP10 binds BECN1.
| 3 4
| 3 2

sparser
"On the other hand, the interaction of Beclin1 and USP10 is consistent with that of enzyme/substrate which is expected to be weak and transient in nature."

reach
"On the other hand, the interaction of Beclin1 and USP10 is consistent with that of enzyme and substrate which is expected to be weak and transient in nature."

sparser
"However, the interaction of Beclin1 and USP10 was considerably weaker (data not shown)."

reach
"However, the interaction of Beclin1 and USP10 was considerably weaker (data not shown)."

reach
"To determine the mechanism by which Vps34 complexes regulate the stability of USP13 and USP10, we examined the possibility that Beclin1 may interact with USP10 and USP13."
| 2

sparser
"To determine the mechanism by which Vps34 complexes regulate the stability of USP13 and USP10, we examined the possibility that Beclin1 may interact with USP10 and USP13."

sparser
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."
USP10 activates BECN1.
| 5
USP10 activates BECN1. 5 / 5
| 5

reach
"Spautin-1 has been recently reported to prevent autophagy by inhibiting the deubiquitinases USP10 and USP13, which leads to beclin 1 and Vps34 (or PI3KIII) degradation [XREF_BIBR]."

reach
"Spautin-1, a potent small molecule, can promote the degradation of PI3KCIII and Vps34 complexes by inhibiting two ubiquitin specific peptidases, USP10 and USP13 which target the Beclin-1 subunit of Vp[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"XREF_BIBR Spautin-1 promotes the degradation of BECN1 by inhibiting 2 USPs (ubiquitin specific peptidases), USP10 and USP13, which target BECN1."

reach
"The fact that USP10 also positively regulates beclin-1 and AMPK would make pharmacologic activators of USP10 particularly effective as autophagy enhancers."

reach
"This molecule promotes the degradation of Vps34-PI 3 kinase complexes by inhibiting two ubiquitin specific peptidases, USP10 and USP13, that target the Beclin-1 subunit of Vps34 complexes."
USP10 affects AMPK_alpha
| 18 5
USP10 binds AMPK_alpha.
| 7 5
| 7 2

reach
"USP10 interacts with AMPK and deubiquitinates AMPKalpha."

reach
"As result, AMPKalpha interacts with USP10 and significantly inhibits polyubiquitylation of AMPKalpha in Hela cells."

reach
"Mechanistically, USP10 interacts and stabilizes PTEN and AMPKalpha by inhibiting their polyubiquitylation."

sparser
"As result, AMPKα interacts with USP10 and significantly inhibits polyubiquitylation of AMPKα in Hela cells ()."

sparser
"As USP10 binds to AMPKα and regulates AMPK activation, we hypothesized that USP10 might regulate AMPK activation through the deubiquitination of AMPKα."

reach
"Mechanistically, USP10 interacts with and stabilizes PTEN and AMPKalpha by inhibiting their polyubiquitylation, and subsequently suppresses the activity of mTOR signaling pathway."

reach
"Inversely, endogenous PTEN and AMPKalpha interacted with endogenous USP10 in HCC-LM3 and HUH7 cells."

reach
"Therefore, we conclude that USP10 interacts with PTEN and AMPKalpha, inhibits their polyubiquitylation, and as a result stabilizes PTEN and AMPKalpha.Next, we examined whether Rapamycin, a well known [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"As USP10 binds to AMPKalpha and regulates AMPK activation, we hypothesized that USP10 might regulate AMPK activation through the deubiquitination of AMPKalpha."
| 3

sparser
"Therefore, we conclude that USP10 interacts with PTEN and AMPKα, inhibits their polyubiquitylation, and as a result stabilizes PTEN and AMPKα."

sparser
"To further dissect how USP10 inhibits mTOR activity, we investigated whether the stabilization of PTEN [] and AMPKα [] by USP10 involved the physical interactions of USP10 with PTEN and AMPKα."

sparser
"Inversely, endogenous PTEN and AMPKα interacted with endogenous USP10 in HCC-LM3 and HUH7 cells (B)."
USP10 deubiquitinates AMPK_alpha.
| 9
USP10 deubiquitinates AMPK_alpha. 9 / 9
| 9

reach
"Mechanistically, following the energy deprivation, USP10 largely deubiquitinates AMPKalpha so that its activating phosphorylation by LKB1 could be greatly enhanced."

reach
"As shown in XREF_FIG and XREF_SUPPLEMENTARY, knockdown of USP10 increased the ubiquitination of WT AMPKalpha; on the other hand, knockdown of USP10 did not affect ubiquitination levels of the AMPKalpha 4KR mutant (XREF_FIG and XREF_SUPPLEMENTARY)."

reach
"We further overexpressed WT USP10 or catalytic inactive mutant in USP10 deficient cells and found that WT USP10, but not the CA mutant decreased ubiquitination of AMPKalpha (XREF_FIG and XREF_SUPPLEMENTARY), suggesting that USP10 regulates AMPKalpha ubiquitination in cells."

reach
"In this study, we identified that USP10 can deubiquitinate PTEN and AMPKalpha and the stabilization of these two proteins will subsequently suppress the activation of AKT and mTOR in HCC cells."

reach
"As shown in Figures 3 E and S3 D, knockdown of USP10 increased the ubiquitination of WT AMPKalpha; on the other hand, knockdown of USP10 did not affect ubiquitination levels of the AMPKalpha 4KR mutan[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"We further overexpressed WT USP10 or CA mutant in USP10 deficient cells and found that WT USP10, but not the CA mutant, decreased ubiquitination of AMPKalpha, suggesting that USP10 regulates AMPKalpha[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"USP10 interacts with AMPK and deubiquitinates AMPKalpha."

reach
"In addition, in line with our study, two different groups have also reported that USP10 deubiquitinates and stabilizes PTEN and AMPKalpha in lung cancer and colon cancer cells [16, 27]."

reach
"Recently, it was established that USP10 deubiquitinates AMPKalpha to promote its interaction with LKB1, its master regulator and thus enhances its activity."
USP10 phosphorylates AMPK_alpha.
| 2
USP10 leads to the phosphorylation of AMPK_alpha. 2 / 2
| 2

reach
"As shown in Figures 3 H and S3 G, knockdown of USP10 decreased the phosphorylation of WT AMPKalpha and disrupted the LKB1-AMPK binding."

reach
"As shown in XREF_FIG and XREF_SUPPLEMENTARY, knockdown of USP10 decreased the phosphorylation of WT AMPKalpha and disrupted the LKB1-AMPK binding."
USP10 affects cell growth
| 1 19
USP10 inhibits cell growth.
| 1 18
| 1 17

reach
"In addition, USP10 suppresses the tumour cell growth in cells with wild-type p53, and USP10 expression is down-regulated in a high percentage of clear cell carcinomas known to have few p53 mutations [108]."

reach
"Downregulation of p53 abolished the inhibitory effect of USP10 on tumor growth (XREF_FIG and data not shown), confirming that USP10 inhibits cancer cell growth by stabilizing p53."

reach
"In addition, USP10 suppresses the tumour cell growth in cells with wild-type p53, and USP10 expression is down-regulated in a high percentage of clear cell carcinomas known to have few p53 mutations [XREF_BIBR]."

reach
"Given USP10 also regulates p53 expression and suppresses tumor cell growth, phosphorylation of USP10 under energy stress might contribute to p53 level increase and tumor suppression."

reach
"On the other hand, shRNA mediated USP10 knockdown caused more vigorous tumor cell growth of both wildtype and p53-null cells, confirming the tumor-suppressive function of USP10 (XREF_FIG, XREF_SUPPLEMENTARY)."

reach
"Moreover, USP10 inhibited cell growth and invasion in lung cancer [XREF_BIBR], and was an independent factor for the prognosis of gastric carcinoma [XREF_BIBR]."

reach
"Consistent with this study, we show that increased USP10 expression in mutant p53 background increases p53 levels and promotes cancer cell proliferation, while downregulation of USP10 inhibits cancer cell growth."

reach
"Our study demonstrated that USP10 suppresses tumor cell growth through potentiating both SIRT6- and p53 mediated suppression of the oncogene c-myc."

reach
"The same study shows that USP10 suppresses tumor cell growth in cells expressing wild-type p53."

reach
"For example, USP10 inhibits lung cancer cell growth and invasion."
Modified USP10 inhibits cell growth. 1 / 1
| 1

reach
"Consistent with this study, we show that increased USP10 expression in mutant p53 background increases p53 levels and promotes cancer cell proliferation, while downregulation of USP10 inhibits cancer cell growth."
USP10 activates cell growth.
| 1
| 1

reach
"On the other hand, in cancer cells with mutant p53, USP10 could promote cancer cell growth, and inhibition of USP10 is called for."
USP10 affects SMAD4
| 10 12
USP10 binds SMAD4.
| 6 12
| 6 12

reach
"Further evidence revealed that USP10 directly binds to Smad4 and stabilizes Smad4 through deubiquitination [46]."

sparser
"Therefore, these results collectively revealed the specific interaction between USP10 and Smad4 both at the endogenous and exogenous levels."

reach
"As USP10 directly binds to Smad4 and regulates its protein stability, we hypothesized that USP10 exerts such regulation through the deubiquitination of Smad4."

sparser
"Further evidence revealed that USP10 directly binds to Smad4 and stabilizes Smad4 through deubiquitination [46] ."

sparser
"As USP10 directly binds to Smad4 and regulates its protein stability, we hypothesized that USP10 exerts such regulation through the deubiquitination of Smad4."

sparser
"USP10 interacts with Smad4 in vivo ."

sparser
"USP10 directly interacts with SMAD4 and stabilizes it to promote HCC proliferation and metastasis (149) ."

reach
"Further analysis showed that USP10 directly interacts with Smad4 and stabilizes it through the cleavage of its proteolytic ubiquitination, thus promoting HCC metastasis."

reach
"More importantly, the interaction between the endogenous USP10 and endogenous Smad4 was further demonstrated in our Co‐IP analyses (Fig. 3D)."

sparser
"In addition, we also observed an interaction between the Flag‐tagged Smad4 and endogenous USP10 (Fig. xref C)."
USP10 increases the amount of SMAD4.
| 2
USP10 increases the amount of SMAD4. 2 / 2
| 2

reach
"We found that depletion of USP10 using USP10‐specific shRNA in HepG2 and Bel‐7402 cells significantly decreased Smad4 protein levels, but had little effect on Smad2/3 protein levels (Fig. 2A,B and Fig. S3A)."

reach
"Conversely, overexpression of USP10‐WT (the wild‐type of USP10) but not USP10‐C424A (the catalytically deficient mutant of USP10) in HepG2 and Bel‐7402 cells dramatically increased the protein levels of Smad4, but not that of Smad2/3 (Fig. 2C,D and Fig. S3C)."
USP10 activates SMAD4.
| 2
USP10 activates SMAD4. 2 / 2
| 2

reach
"We then explored the mechanism by which USP10 modulates Smad4 stability, firstly by examining whether USP10 is a bona fide Smad4 binding protein."

reach
"USP10 promotes hepatocellular cancer metastasis by stabilizing Smad4 protein [20]."
PCNA affects USP10
1 | 9 12
1 | 9 12

reach
"These results indicate that USP10 preferentially binds to ISGylated PCNA to cleave off mono-ubiquitin for TLS termination.Significantly, USP10 interacted with PCNA when doubly ISGylated PCNA appeared [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Significantly, USP10 interacted with PCNA when doubly ISGylated PCNA appeared (i.e., at 24 hr after UV treatment), but not when PCNA was mono-ISGylated (i.e., at 12 hr) ( Figure 3 J)."

sparser
"Figure 3 H shows that USP10 binds to ISGylated PCNA much more tightly than to its unmodified form."

sparser
"These results indicate that USP10 preferentially binds to ISGylated PCNA to cleave off mono-ubiquitin for TLS termination."

reach
"Moreover, PCNA binding to USP10 was abrogated by K168R mutation or ISG15 knockdown, both of which block PCNA ISGylation."

reach
"This important question awaits further studies.Surprisingly, the binding of USP10 to unmodified endogenous PCNA could not be detected, despite the fact that it has a noncanonical PIP box."

sparser
"ISGylated PCNA promotes the interaction between PCNA and USP10 and de-ubiquitination of mono-ubiquitinated PCNA."

reach
"Ubiquitylated PCNA promotes its ISGylation 12 h after UV irradiation [228] and the ISGylated PCNA binds to USP10, which then removes ubiquitin from PCNA to stop TLS [228]."

reach
"Likewise, the binding of USP10 to PCNA via its noncanonical PIP box might be very weak, but it could be markedly enhanced by PCNA ISGylation, and this enhancement might provide the ability of USP10 to[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Likewise, the binding of USP10 to PCNA via its noncanonical PIP box might be very weak, but it could be markedly enhanced by PCNA ISGylation, and this enhancement might provide the ability of USP10 to[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
HDAC6 affects USP10
| 6 16
| 6 16

sparser
"To determine whether USP10 binds to HDAC6 directly or via other proteins, we generated and purified recombinant GST-USP10 and His-HDAC6 in E. coli ."

sparser
"Implications of the USP10-HDAC6 axis in lung cancer - A path to precision medicine."

sparser
"Finally, as the current landscape for the treatment of advanced lung cancer has shifted towards anti-PD-l/PD-L1-based immunotherapy [ xref ], further investigations to connect the USP10-HDAC6 axis to immunotherapy are certainly warranted."

sparser
"Overall, our data suggest targeting the USP10-HDAC6 axis in NSCLC lacking wild-type p53 (Fig. xref )."

sparser
"USP10 domain mapping found a more restricted binding pattern, as besides the full-length, the very C-terminal region (amino acids 700–798) was the only one necessary for the interaction between USP10 and HDAC6 to occur (Fig. xref )."

reach
"In the null-p53 or mutant-p53 background, USP10 may largely bind to HDAC6."

reach
"USP10 interacts with HDAC6."

reach
"Next, we mapped which domain (s) of HDAC6 binds to USP10, and vice versa."

sparser
"Our investigations then revealed that HDAC6 interacts with USP10, and it is a substrate of USP10."

reach
"To determine whether USP10 binds to HDAC6 directly or via other proteins, we generated and purified recombinant GST-USP10 and His HDAC6 in E. coli."
USP10 affects AICDA
| 16 5
USP10 binds AICDA.
| 3 5
| 3 5

sparser
"Furthermore, the specific interaction between internal USP10 and AID was confirmed in CH12 cells (Fig. xref )."

reach
"The interaction of internal USP10 and AID was determined with IP and western blot assay."

sparser
"Furthermore, the specific interaction between internal USP10 and AID was confirmed in CH12 cells (Fig. 1d) ."

sparser
"The interaction of internal USP10 and AID was determined with IP and western blot assay."

reach
"Besides, the GST pull-down assay confirmed that AID directly bound with USP10 (Supplementary Fig. 1f)."

sparser
"Besides, the GST pull-down assay confirmed that AID directly bound with USP10 ( Supplementary Fig. 1f )."

reach
"Furthermore, the specific interaction between internal USP10 and AID was confirmed in CH12 cells (Fig. 1d)."

sparser
"Besides, the GST pull-down assay confirmed that AID directly bound with USP10 (Supplementary Fig. xref )."
USP10 deubiquitinates AICDA.
| 5
USP10 deubiquitinates AICDA. 5 / 5
| 5

reach
"USP10 regulates B cell response to SARS-CoV-2 or HIV-1 nanoparticle vaccines through deubiquitinating AID."

reach
"l USP10 overexpression decreased AID ubiquitination."

reach
"k USP10 knockdown promoted AID ubiquitination."

reach
"As shown in Fig. 1e, k, knockdown of USP10 disrupted AID stability and increased AID ubiquitination."

reach
"Alternatively, the overexpression of USP10, but not its catalytically inactive CA mutant (C424A), stabilized AID and decreased the ubiquitination of AID (Fig. 1f, l) ."
USP10 activates AICDA.
| 5
USP10 activates AICDA. 5 / 5
| 5

reach
"h USP10 knockdown promoted AID degradation."

reach
"Collectively, we demonstrated that USP10 functions as an integrator for both BCR and TLR signals and directly regulates nuclear AID activity."

reach
"The relative fluorescence signal was calculated through fluorescence intensity at a certain time divided by fluorescence intensity at 0 h. i The USP10 overexpression inhibited AID degradation."

reach
"Collectively, these results indicated that BCR and TLR2 synergistically enhance Akt activity and promote USP10 T674 phosphorylation located within the NLS domain and, therefore, the nuclear import of USP10, consequently inhibiting the AID degradation in the nucleus.Pam 3 Csk 4 helped to enhance the antibody response to the SARS-CoV-2 RBD nanoparticle vaccine Pam 3 Csk 4 has been used as a vaccine adjuvant."

reach
"These results indicated that USP10 mainly inhibited AID degradation in the nucleus and affected the AID abundance in the cytoplasm.The concentration of nuclear AIDs is important."
USP10 increases the amount of AICDA.
| 3
USP10 increases the amount of AICDA. 3 / 3
| 3

reach
"c The fluorescence-field micrograph of identified AID-interacting deubiquitinase siRNA screen showed that USP10 knockdown dramatically decreased AID-GFP levels."

reach
"These results suggest that USP10 knockout in B cells decreases AID expression in B cells, thereby, decreasing IgG1 and IgA class-switch.AID is required for SHM and antibody affinity maturation."

reach
"The deficiency of USP10 in B cells significantly decreased AID protein levels, subsequently reducing neutralizing antibody production after immunization with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or human immunodeficiency virus type 1 (HIV-1) nanoparticle vaccines."
CFTR affects USP10
3 | 7 9
3 | 7 9

sparser
"Here, we extend our previous study, conducted using a stably transformed airway epithelial cell line, by demonstrating that USP10 associates with CFTR in primary cultures of polarized human bronchial epithelial cells."

sparser
"Moreover, USP10 interacted with CFTR in the early endosomes, an important requirement for a DUB to regulate a target protein."

reach
"XREF_BIBR, XREF_BIBR Thus, if USP10 regulates the deubiquitination of CFTR, we would predict that USP10 should interact with CFTR in the early endosomal compartment of HBE cells."

sparser
"The ability of USP10 to deubiquitinate target proteins requires interaction between USP10 and its substrate. xref , xref Thus, if USP10 regulates the deubiquitination of CFTR, we would predict that USP10 should interact with CFTR in the early endosomal compartment of HBE cells."

reach
"Three observations suggest that Cif may inhibit USP10 activity by stabilizing the interaction between USP10 and G3BP1, which inhibits USP10 DUB activity, and also reduces the interaction between USP10 and CFTR."

sparser
"As shown in xref , USP10 interacts with CFTR in the early endosomes isolated from airway epithelial cells, and Cif reduces this interaction by 50±6%."

reach
"Thus, studies were conducted to determine if Cif inhibits USP10 activity by stabilizing the interaction between USP10 and G3BP1, and by reducing the interaction between USP10 and CFTR."

sparser
"In the current study, we show localization and activity of USP10 in the early endosomal compartment of primary bronchial epithelial cells, as well as an interaction between CFTR and USP10 in this compartment."

sparser
"Thus, USP10 and CFTR interact in the early endosomal compartment of polarized primary human bronchial epithelial cells."

sparser
"Thus, studies were conducted to determine if Cif inhibits USP10 activity by stabilizing the interaction between USP10 and G3BP1, and by reducing the interaction between USP10 and CFTR."
ZC3H12A affects USP10
3 | 7 9
3 | 4 2

sparser
"They found that MCPIP1 directly binds to USP10 and serves as a bridge between USP10 and TANK [33] ."

No evidence text available

No evidence text available

sparser
"MCPIP1 associated with USP10 (Ubiquitin Specific Peptidase 10) after genotoxic stress, enhancing the latter’s deubiquitinase activity, thereby producing deubiquitination of NEMO (IKK-γ) which resulted in inhibition of NF-κB activation ( xref )."

reach
"They found that MCPIP1 directly binds to USP10 and serves as a bridge between USP10 and TANK [33]."

No evidence text available

reach
"Altogether, these data support that TANK may also serve as a negative regulator of genotoxic NF-kappaB activation by directing USP10 and MCPIP1 complex to polyubiquitinated TRAF6."

reach
"MCPIP1 associated with USP10 (Ubiquitin Specific Peptidase 10) after genotoxic stress, enhancing the latter 's deubiquitinase activity, thereby producing deubiquitination of NEMO (IKK-gamma) which resulted in inhibition of NF-kappaB activation."

reach
"MCPIP1 forms a complex with USP10 and TANK, which mediates the deubiquitination of the TRAF6 K63 chain [76] and inhibits NF-κB activation upon DNA damage [33]."
| 3 2

reach
"Genotoxic stress also produced a TANK, MCPIP1, and USP10 complex that effected and enhanced deubiquitination of both TRAF6 (E3 Ubiquitin Protein Ligase) and NEMO, which in turn inhibited NF-kappaB activation."

sparser
"MCPIP1 forms a complex with USP10 and TANK, which mediates the deubiquitination of the TRAF6 K63 chain [76] and inhibits NF-κB activation upon DNA damage [33] ."

sparser
"Intriguingly, TANK was identified as a MCPIP1-interacting protein in a proteomic screen and we confirmed that TANK forms a complex with MCPIP1 and USP10."

reach
"Furthermore, we found that the TANK, MCPIP1, and USP10 complex also decreased TRAF6 ubiquitination in cells treated with IL-1beta or LPS."

reach
"In addition to genotoxic stress, the TANK, MCPIP1, and USP10 complex is also responsible for restraining TRAF6 ubiquitination in cells treated with LPS or IL-1beta."
| 4

sparser
"NEMO ubiquitylation is decreased through the deubiquitinase USP10, which interacts with NEMO via MCPIP1 upon genotoxic stress."

sparser
"Moreover, usp4-depleted zebrafish (Danio rerio) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment. [102] Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) cleaves K63-linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to highrisk human papillomavirus (hrHPV) infection. [103] TRAF family member-associated NF-B activator (TANK) interacts with both monocyte chemotactic protein-1-induced protein-1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF-B activation in response to TLR activation. [104] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO-attached M1-linked polyubiquitin chains, thus inhibiting the genotoxic NF-B signaling cascade. [40] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."

sparser
"Moreover, usp4 ‐depleted zebrafish ( Danio rerio ) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment. [ xref ] Ubiquitin carboxyl‐terminal hydrolase L1 (UCHL1) cleaves K63‐linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to high‐risk human papillomavirus (hrHPV) infection. [ xref ] TRAF family member‐associated NF‐ κ B activator (TANK) interacts with both monocyte chemotactic protein‐1‐induced protein‐1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF‐ κ B activation in response to TLR activation. [ xref ] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO‐attached M1‐linked polyubiquitin chains, thus inhibiting the genotoxic NF‐ κ B signaling cascade. [ xref ] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."

sparser
"Moreover, usp4‐depleted zebrafish (Danio rerio) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment.[ 102 ] Ubiquitin carboxyl‐terminal hydrolase L1 (UCHL1) cleaves K63‐linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to high‐risk human papillomavirus (hrHPV) infection.[ 103 ] TRAF family member‐associated NF‐κB activator (TANK) interacts with both monocyte chemotactic protein‐1‐induced protein‐1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF‐κB activation in response to TLR activation.[ 104 ] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO‐attached M1‐linked polyubiquitin chains, thus inhibiting the genotoxic NF‐κB signaling cascade.[ 40 ] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."
| 1

sparser
"TRAF family member-associated NF- κ B activator (TANK) interacts with both MCPIP1 and USP10, which leads to decrease in TRAF6 ubiquitination and the termination of the NF- κ B activation in response to TLR activation [ xref ]."
USP10 affects cell death
| 15 5
USP10 inhibits cell death.
| 10 5
| 10 5

sparser
"USP10 inhibited cell death induced by PI, and the inhibition correlated with aggresome/aggregate-inducing activity ( xref and xref )."

sparser
"Thus, we examined whether USP10 inhibits MG-132-induced cell death by measuring activated caspase-3 (cleaved caspase-3)."

reach
"USP10 inhibited cell death induced by PI, and the inhibition correlated with aggresome and aggregate inducing activity (XREF_FIG and XREF_SUPPLEMENTARY)."

reach
"Similar to Beclin 1 knockdown, we found that suppression of the ubiquitin specific peptidase, USP10, or a small molecule inhibitor of the deubiquitinases USP10 and USP13, i.e., spautin-1 XREF_BIBR, can increase radiation induced DSBs and promote tumor cell death."

sparser
"To obtain information describing how USP10 inhibits MG-132-induced cell death, we measured cell death of USP10-KD cells expressing several USP10 mutants."

reach
"These results indicated that USP10 and p62 cooperatively inhibit MG-132-induced cell death by promoting the formation of aggresomes and p62 aggregates."

reach
"To obtain information describing how USP10 inhibits MG-132-induced cell death, we measured cell death of USP10-KD cells expressing several USP10 mutants."

reach
"Given that USP10-WT, USP10 C424A, and USP10 96-798, but not USP10 1-214, in USP10-KD cells promoted p62 aggregation and aggresome formation (XREF_FIG G and XREF_SUPPLEMENTARY A-S5C), these results suggested that USP10 inhibits cell death induced by MG-132 by promoting aggresome formation and p62 aggregation."

reach
"Thus, we examined whether USP10 inhibits MG-132-induced cell death by measuring activated caspase-3 (cleaved caspase-3)."

reach
"USP10 Inhibits Cell Death Induced by MG-132."
USP10 activates cell death.
| 5
| 5

reach
"Consistent with our screening results on the effect of USP10 siRNA, pretreatment with spautin-1, a specific inhibitor of USP10, markedly inhibited curcumin induced morphological changes of mitochondria and subsequent paraptotic cell death in various breast cancer cells."

reach
"Additional work is needed to investigate the detailed underlying mechanism by which USP10 contributes to mitochondrial dilation and subsequent paraptotic cell death in malignant breast cancer cells."

reach
"In this report, we found that PdPT is an inhibitor of multiple DUBs including USP7, USP10, USP14, USP15, USP25 and UCHL5, which contributes to the accumulation of ubiquitinated proteins and subsequent cell death in NSCLC cell lines.Regulated cell death requires activation of various regulators and effectors (23)."

reach
"Similar to Beclin 1 knockdown, we found that suppression of the ubiquitin specific peptidase, USP10, or a small molecule inhibitor of the deubiquitinases USP10 and USP13, i.e., spautin-1 XREF_BIBR, can increase radiation induced DSBs and promote tumor cell death."

reach
"Additional work will be needed to investigate the detailed underlying mechanism by which USP10 contributes to paraptotic cell death in malignant breast cancer cells."
USP10 affects ZC3H12A
3 | 7 9
3 | 4 2

sparser
"They found that MCPIP1 directly binds to USP10 and serves as a bridge between USP10 and TANK [33] ."

No evidence text available

No evidence text available

sparser
"MCPIP1 associated with USP10 (Ubiquitin Specific Peptidase 10) after genotoxic stress, enhancing the latter’s deubiquitinase activity, thereby producing deubiquitination of NEMO (IKK-γ) which resulted in inhibition of NF-κB activation ( xref )."

reach
"They found that MCPIP1 directly binds to USP10 and serves as a bridge between USP10 and TANK [33]."

No evidence text available

reach
"Altogether, these data support that TANK may also serve as a negative regulator of genotoxic NF-kappaB activation by directing USP10 and MCPIP1 complex to polyubiquitinated TRAF6."

reach
"MCPIP1 associated with USP10 (Ubiquitin Specific Peptidase 10) after genotoxic stress, enhancing the latter 's deubiquitinase activity, thereby producing deubiquitination of NEMO (IKK-gamma) which resulted in inhibition of NF-kappaB activation."

reach
"MCPIP1 forms a complex with USP10 and TANK, which mediates the deubiquitination of the TRAF6 K63 chain [76] and inhibits NF-κB activation upon DNA damage [33]."
| 3 2

reach
"Genotoxic stress also produced a TANK, MCPIP1, and USP10 complex that effected and enhanced deubiquitination of both TRAF6 (E3 Ubiquitin Protein Ligase) and NEMO, which in turn inhibited NF-kappaB activation."

sparser
"MCPIP1 forms a complex with USP10 and TANK, which mediates the deubiquitination of the TRAF6 K63 chain [76] and inhibits NF-κB activation upon DNA damage [33] ."

sparser
"Intriguingly, TANK was identified as a MCPIP1-interacting protein in a proteomic screen and we confirmed that TANK forms a complex with MCPIP1 and USP10."

reach
"Furthermore, we found that the TANK, MCPIP1, and USP10 complex also decreased TRAF6 ubiquitination in cells treated with IL-1beta or LPS."

reach
"In addition to genotoxic stress, the TANK, MCPIP1, and USP10 complex is also responsible for restraining TRAF6 ubiquitination in cells treated with LPS or IL-1beta."
| 4

sparser
"NEMO ubiquitylation is decreased through the deubiquitinase USP10, which interacts with NEMO via MCPIP1 upon genotoxic stress."

sparser
"Moreover, usp4-depleted zebrafish (Danio rerio) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment. [102] Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) cleaves K63-linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to highrisk human papillomavirus (hrHPV) infection. [103] TRAF family member-associated NF-B activator (TANK) interacts with both monocyte chemotactic protein-1-induced protein-1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF-B activation in response to TLR activation. [104] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO-attached M1-linked polyubiquitin chains, thus inhibiting the genotoxic NF-B signaling cascade. [40] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."

sparser
"Moreover, usp4 ‐depleted zebrafish ( Danio rerio ) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment. [ xref ] Ubiquitin carboxyl‐terminal hydrolase L1 (UCHL1) cleaves K63‐linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to high‐risk human papillomavirus (hrHPV) infection. [ xref ] TRAF family member‐associated NF‐ κ B activator (TANK) interacts with both monocyte chemotactic protein‐1‐induced protein‐1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF‐ κ B activation in response to TLR activation. [ xref ] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO‐attached M1‐linked polyubiquitin chains, thus inhibiting the genotoxic NF‐ κ B signaling cascade. [ xref ] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."

sparser
"Moreover, usp4‐depleted zebrafish (Danio rerio) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment.[ 102 ] Ubiquitin carboxyl‐terminal hydrolase L1 (UCHL1) cleaves K63‐linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to high‐risk human papillomavirus (hrHPV) infection.[ 103 ] TRAF family member‐associated NF‐κB activator (TANK) interacts with both monocyte chemotactic protein‐1‐induced protein‐1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF‐κB activation in response to TLR activation.[ 104 ] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO‐attached M1‐linked polyubiquitin chains, thus inhibiting the genotoxic NF‐κB signaling cascade.[ 40 ] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."
| 1

sparser
"TRAF family member-associated NF- κ B activator (TANK) interacts with both MCPIP1 and USP10, which leads to decrease in TRAF6 ubiquitination and the termination of the NF- κ B activation in response to TLR activation [ xref ]."
| 20

reach
"Further study demonstrated that USP10 promotes TGF‐β signaling and HCC metastasis through the regulation on the protein abundance of Smad4, but imposing minimal effect on Smad2 and Smad3."

reach
"Deubiquitinating enzyme USP10 promotes osteosarcoma metastasis and epithelial-mesenchymal transition by stabilizing YAP1."

reach
"USP10 promotes hepatocellular cancer metastasis by stabilizing Smad4 protein [20]."

reach
"Deubiquitinase USP10 maintains Cyr61 expression via YAP1 to augment immune escape and metastasis of PAAD."

reach
"Targeting USP10 by shRNA inhibited CRC proliferation and metastasis, and this effect was reversed by overexpression of NLRP7."

reach
"In the current study, we found that there was no correlation between expression of TM4SF1 and USP10, S100A12, p53, or Ki67 expression in GC, suggesting that TM4SF1 plays a distinct role in GC invasion and metastasis from those of USP10, S100A12, p53, or Ki67, although they all contribute to cancer invasion and metastasis."

reach
"As shown in Fig. S5D, the protein levels of USP10 were more abundant and highly correlated with Smad4 expression in tumorous regions compared with that in nontumorous tissues.These results demonstrate that USP10 reinforces the TGF‐β signaling and promotes the metastasis of HCC cells."

reach
"In order to demonstrate that Smad4 indeed involved in the regulation of USP10 promoting HCC metastasis, we investigated whether Smad4 could rescue the metastasis defect caused by USP10 depletion."

reach
"Moreover, ablation of USP10 by either shRNAs or USP10 inhibitor Spautin‐1 significantly suppresses the metastasis of HCC cells in vitro and/or in vivo, whereas the reconstitution of Smad4 was able to efficiently rescue this defect, indicating that targeting USP10 could be a novel therapeutic strategy of metastatic HCC displaying high level of Smad4.2 Materials and methods."

reach
"To extend our in vitro observations concerning USP10’s role in promoting HCC metastasis, we evaluated whether USP10 could promote HCC metastasis in vivo."
USP10 bound to it activates Neoplasm Metastasis. 2 / 2
| 2

reach
"Further analysis showed that USP10 directly interacts with Smad4 and stabilizes it through the cleavage of its proteolytic ubiquitination, thus promoting HCC metastasis."

reach
"USP10 directly interacts with SMAD4 and stabilizes it to promote HCC proliferation and metastasis (149)."
G3BP2 affects USP10
5 | 2 13
5 | 2 3

sparser
"For example, USP10 interacts with G3BP2 to block p53 signal transduction, leading to poor prognosis in prostate cancer [ xref ]; USP44 promotes the development of prostate cancer by stabilizing EZH2 [ xref ], and USP2a enhances c-Myc expression via microRNA-related regulation and thus promotes tumourigenesis [ xref ]."

sparser
"The association of USP10 with G3BP2 suppresses p53 signalling which correlates to a poor prognosis in prostate cancer, highlighting an oncogenic role of USP10 in prostate cancer via G3BP2 [ xref ]."

No evidence text available

No evidence text available

reach
"For example, USP10 interacts with G3BP2 to block p53 signal transduction, leading to poor prognosis in prostate cancer [XREF_BIBR]; USP44 promotes the development of prostate cancer by stabilizing EZH2 [XREF_BIBR], and USP2a enhances c-Myc expression via microRNA related regulation and thus promotes tumourigenesis [XREF_BIBR]."

No evidence text available

sparser
"The N protein compromised G3BP1-USP10 and 345 G3BP1-PKR interactions (Fig. 4b, c) and disrupted G3BP2-USP10 and G3BP2-PKR interactions 346 (Fig. 4d, e) ."

reach
"Furthermore, USP10, a binding partner of G3BP2, is also an androgen responsive gene and is transcriptionally activated by the AR."

No evidence text available

No evidence text available
| 6

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [ xref ], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [ xref ]."

sparser
"However, a role for the G3BP1 and G3BP2 interactions with CAPRIN1 and/or USP10 under chronic nutrient starvation has not yet been investigated."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al.2017); SeV Trailer RNA captures TIAR from SG (Iseni et al.2002); West Nile Virus (WNV) and Dengue virus (DENV) 3′-end viral genome captures TIA-1/TIAR (Li et al.2002; Emara and Brinton 2007; Xia et al.2015); DENV 3′-UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al.2011; Reineke et al.2015); JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al.2011)."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al. 2017) ; SeV Trailer RNA captures TIAR from SG (Iseni et al. 2002) ; West Nile Virus (WNV) and Dengue virus (DENV) 3 0 -end viral genome captures TIA-1/TIAR (Li et al. 2002; Emara and Brinton 2007; Xia et al. 2015) ; DENV 3 0 -UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al. 2011; Reineke et al. 2015) ; JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al. 2011) ."

sparser
"Additional experiments demonstrate that, in contrast to DDX6, the SG proteins G3BP1, G3BP2, Caprin1 and USP10 bind to the variable region (VR) in the 3' UTR."

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [171], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [172]."
| 4

sparser
"Validation of the mass spectrometry data confirmed the interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."

sparser
"NCAP showed specific interaction with the SG proteins G3BP1, G3BP2, YTHDF3, USP10 and PKR, and translocated to SGs following oxidative stress and heat shock."

sparser
"NCAP interacts with SG proteins. (A) Interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."

sparser
"Fig. 4: NCAP interacts with SG proteins. (A) Interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."
USP13 affects USP10
1 | 10 5
USP13 binds USP10.
| 4 5
| 4 3

sparser
"USP13 also interacts with USP10, and the interaction increases USP13 deubiquitinating activity ( xref )."

reach
"Although no structural information for USP13 and USP10 is currently available, the enhanced DUB activity when USP13 interacts with USP10 or when they interact with Beclin1 suggest that the interaction of USP13 and USP10 with each other or with their substrates can lead to changes in their conformation which may be critical for the catalytic activities."

reach
"Using spautin-1 as a tool, we explored the interaction of USP10 and USP13 with Vps34 complexes."

reach
"USP13 also interacts with USP10, and the interaction increases USP13 deubiquitinating activity."

sparser
"Although no structural information for USP13 and USP10 is currently available, the enhanced DUB activity when USP13 interacts with USP10 or when they interact with Beclin1 suggest that the interaction of USP13 and USP10 with each other or with their substrates can lead to changes in their conformation which may be critical for the catalytic activities."

reach
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."

sparser
"No orthogonal biophysical methods, such as the surface plasmon response (SPR) or isothermal titration calorimetry (ITC), were used to validate the binding of spautin-1 with USP10 or USP13."
| 2

sparser
"To determine the mechanism by which Vps34 complexes regulate the stability of USP13 and USP10, we examined the possibility that Beclin1 may interact with USP10 and USP13."

sparser
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."
USP13 deubiquitinates USP10.
1 | 5
USP13 deubiquitinates USP10. 6 / 6
1 | 5

reach
"Moreover, beclin-1 and USP10 are involved in a potential feedforward mechanism in which beclin-1 stabilizes USP13, which in turn deubiquitinates and stabilizes USP10, leading to increased beclin-1 levels and activity."

No evidence text available

reach
"Since USP13 can also deubiquitinate USP10, regulating the stability of USP13 by Beclin1 provides a mechanism for Beclin1 to control the stability of USP10."

reach
"These results suggest that USP13 may directly regulate the deubiquitination of USP10; however, USP10 may regulate USP13 indirectly perhaps by affecting the levels of Vps34 complexes."

reach
"Very interestingly, they also pointed out that beclin-1 controlled the stability of USP10 by regulating the stability of USP13, which can deubiquitinate USP10 (also see Figure 4) [53]."

reach
"Consistent with this possibility, the ubiquitination levels of USP10 were reduced when cells were cotransfected with an expression vector of USP13 and the addition of spautin-1 inhibited the deubiquitination of USP10 by USP13 (XREF_FIG)."
USP13 inhibits USP10.
| 1
USP13 inhibits USP10. 1 / 1
| 1

reach
"It will be interesting to elucidate if the stability of the other PI3K and III complex members is also affected, as in the case of Spautin-1 -- mediated inhibition of USP10 and USP13 [XREF_BIBR] and if the control of Beclin 1 stability is mediated by HSP90 in general or whether this represents a regulatory event specifically developed in phagocytic cells."
USP10 affects FLT3
1 | 12 6
USP10 binds FLT3.
| 2 6
| 2 6

reach
"The data in our report demonstrated that ATO treatment reduces the binding of USP10 to FLT3 protein, which might partly contribute to the enhanced ubiquitination of mutant FLT3 protein.Hsp90 is an important chaperone molecule assisting in folding and preventing client proteins from ubiquitination and degradation [44]."

sparser
"A similar interaction between USP10 and FLT3 was demonstrated in 293T cells engineered to exogenously express these proteins ( xref )."

reach
"Co-immunoprecipitation assays showed that ATO treatment led to significantly reduced binding of USP10 with FLT3 protein in TF1/ITD-Ub cells."

sparser
"Co-immunoprecipitation assays showed that ATO treatment led to significantly reduced binding of USP10 with FLT3 protein in TF1/ITD-Ub cells."

sparser
"The data in our report demonstrated that ATO treatment reduces the binding of USP10 to FLT3 protein, which might partly contribute to the enhanced ubiquitination of mutant FLT3 protein."

sparser
"Our current data also support our previous observation that FLT3 and USP10 bind to one another, xref as FLT3 and USP10 co-immunoprecipitate in Ba/F3-FLT3-ITD cells (an AKT immunoprecipitation was included as a control for nonspecific binding) (Fig.  xref , lower panel)."

sparser
"ATO facilitates poly-ubiquitination and degradation of FLT3 partly through reducing the binding of mutant FLT3 to the deubiquitinating enzyme USP10."

sparser
"We observed robust co-immunoprecipitation (co-I.P.) of USP10 with FLT3 in Ba/F3-FLT3-ITD cells; reverse co-I.P. studies confirmed the association of FLT3 with USP10 ( xref )."
USP10 activates FLT3.
| 7
USP10 activates FLT3. 6 / 6
| 6

reach
"Intriguingly, USP10 inhibition has been shown to promote oncoprotein FLT3 degradation, suggesting that USP10 could be considered as a potential pharmacological target for fighting FLT3- mutant AML disease [XREF_BIBR]."

reach
"USP10 inhibition leads to death of cells driven by active SYK or oncogenic FLT3 and potentiates the anti-leukemic effects of FLT3 inhibition in these cells."

reach
"A more recent study revealed that USP10, a deubiquitinating enzyme, is essential for the stabilization of FLT3 protein and inhibition of USP10 induces the degradation of FLT3/ITD, as well as FLT3/TKD, albeit to a lesser extent [42]."

reach
"Inhibition of USP10 induces degradation of oncogenic FLT3."

reach
"It was recently reported that inhibition of USP10 also induces degradation of FLT3/ITD [42]."

reach
"In addition, we show that USP10 inhibition potentiates the effects of FLT3 inhibition in cells driven by activated SYK."
USP10 activates mutated FLT3. 1 / 1
| 1

reach
"Mechanically, USP10 led to the accumulation of FLT3–ITD through deubiquitinating and stabilizing the mutant FLT3–ITD, promoting AML progression [9,61]."
USP10 deubiquitinates FLT3.
1 | 3
USP10 deubiquitinates FLT3. 2 / 2
1 | 1

"USP10, a primary cytoplasmic DUB, acts as an oncogene or a tumor suppressor by regulating various protein substrates, including FLT3, p53, AMPK, PTEN, etc."

reach
"However, in acute myeloid leukemia (AML) patients with activating mutations in FMS‐like tyrosine kinase 3 (FLT3), USP10 deubiquitinates and stabilizes FLT3 by removing the proteolytic polyubiquitin chains and preventing FLT3 degradation, to aggravate tumor progress (Weisberg et al., 2017)."
USP10 leads to the deubiquitination of mutated FLT3. 2 / 2
| 2

reach
"In addition to mutant FLT3–ITD, another spleen tyrosine kinase (SYK), a critical regulator of FLT3, was deubiquitinated and stabilized by USP10; depleting or inhibiting USP10 reduced the expression of SYK, which promoted the proliferation of Ba/F3 and MOLM14 cells [68,84]."

reach
"Given our recent finding that USP10 deubiquitinates mutant FLT3, 18 coupled with reported studies showing that FLT3 and SYK are interacting partners with each other and are targets of the same E3 ligase c-CBL, XREF_BIBR, XREF_BIBR, XREF_BIBR - XREF_BIBR we hypothesised that USP10 may play a role in stabilisation of SYK."
USP10 affects CD36
| 4 9 6
USP10 binds CD36.
| 4 6
USP10 binds CD36. 6 / 6
| 6

sparser
"Deeply, we sought to the regulation mechanism of CD36-USP10 in macrophages."

sparser
"Besides, we tested the regulation of USP10-CD36 under the oxLDL stimulation."

sparser
"In addition, Co-IP results showed that USP10 interacts with CD36 and stabilizes CD36 protein by cleaving poly-ubiquitin on CD36."

sparser
"Western blot results showed that USP10 interacts with CD36 ( xref , xref )."

sparser
"The regulation of USP10-CD36 may provide a significant therapeutic scheme in atherosclerosis."

sparser
"USP10 interacts with CD36."
| 4

reach
"USP10 interacts with CD36."

reach
"In addition, Co-IP results showed that USP10 interacts with CD36 and stabilizes CD36 protein by cleaving poly-ubiquitin on CD36."

reach
"Co-IP and confocal assays revealed that CD36 interacts USP10 and the co-localization of CD36 and USP10."

reach
"Western blot results showed that USP10 interacts with CD36 (XREF_FIG, XREF_FIG)."
USP10 activates CD36.
| 4 3
USP10 activates CD36. 5 / 5
| 4 1

reach
"In this study, our western blotting and RT-qPCR assays revealed that USP10 inhibition promotes the degradation of CD36 protein but does not change its mRNA level."

eidos
"USP10 knockdown also inhibited the expression of CD36 protein ( Figure 3C , 3D ) ."

eidos
"We found that USP10 inhibitor significantly reduced CD36 expression ( Figure 3E , 3F ) ."

eidos
"Western blot showed that increased expression of CD36 protein was suppressed by USP10 inhibitor or siRNA ( Figure 4A , 4B ) ."

eidos
"In this study , our western blotting and RT-qPCR assays revealed that USP10 inhibition promotes the degradation of CD36 protein but does not change its mRNA level ."
USP10 activates ubiquitinated CD36. 2 / 2
| 2

reach
"As shown in XREF_FIG, XREF_FIG, USP10 inhibition or knockdown significantly increased ubiquitinated CD36."

reach
"Moreover, the K48-poly-ubiquitinated CD36 was upregulated by Spautin-1 or USP10 siRNA."
USP10 increases the amount of CD36.
| 2
USP10 increases the amount of CD36. 2 / 2
| 2

reach
"USP10 knockdown also inhibited the expression of CD36 protein (XREF_FIG, XREF_FIG)."

reach
"We found that USP10 inhibition decreased the expression of CD36 in THP1 cells (XREF_FIG)."
USP10 affects AMPK
| 10 8
USP10 activates AMPK.
| 7 2
USP10 activates AMPK. 9 / 10
| 7 2

reach
"The fact that USP10 also positively regulates beclin-1 and AMPK would make pharmacologic activators of USP10 particularly effective as autophagy enhancers."

sparser
"Finally, USP10 deubiquitinated and activated the autophagy-promoting kinase AMPK [53] ."

reach
"These results suggest that USP10 deubiquitinase activity is required for its effects in AMPK activation and cellular metabolism.We then explored the mechanism by which USP10 modulates AMPK activation,[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Only WT USP10, but not the CA mutant could rescue AMPK activation triggered by USP10 RNAi (XREF_FIG), suggesting that USP10 deubiquitinase activity is required for AMPK activation."

reach
"As shown in Figure 4 B, the phosphorylation of USP10 increased significantly after glucose starvation, suggesting that USP10 might be a substrate of AMPK."

sparser
"Finally, USP10 deubiquitinates and activates the autophagy-promoting kinase AMPK ( xref )."

reach
"We then explored the mechanism by which USP10 modulates AMPK activation, first by examining whether USP10 directly interacts with AMPK."

reach
"Only WT USP10, but not the CA mutant, could rescue AMPK activation triggered by USP10 RNAi, suggesting that USP10 deubiquitinase activity is required for AMPK activation."

reach
"As shown in XREF_FIG, the phosphorylation of USP10 increased significantly after glucose starvation, suggesting that USP10 might be a substrate of AMPK."
USP10 binds AMPK.
| 3 6
| 3 6

reach
"These results suggest that USP10 deubiquitinase activity is required for its effects in AMPK activation and cellular metabolism.We then explored the mechanism by which USP10 modulates AMPK activation,[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"USP10 interacts with AMPK and deubiquitinates AMPKα."

reach
"USP10 interacts with AMPK and deubiquitinates AMPKalpha."

sparser
"We then explored the mechanism by which USP10 modulates AMPK activation, first by examining whether USP10 directly interacts with AMPK."

reach
"We then explored the mechanism by which USP10 modulates AMPK activation, first by examining whether USP10 directly interacts with AMPK."

sparser
"Thus, USP10 and AMPK form a key feedforward loop ensuring amplification of AMPK activation in response to fluctuation of cellular energy status."

sparser
"Using recombinant proteins, we found that USP10 directly interacted with AMPK α1 and α2 but not the other AMPK subunits ( xref )."

sparser
"USP10 and AMPK can form a key feedforward loop that ensures amplification of AMPK activation in response to fluctuations in cellular energy status ( xref )."

sparser
"Using recombinant proteins, we found that USP10 directly interacted with AMPK α1 and α2 but not the other AMPK subunits ( Figure 2 A)."
Spautin-1 affects USP10
| 2 16
Spautin-1 inhibits USP10.
| 2 10
Spautin-1 inhibits USP10. 10 / 10
| 10

reach
"In contrast, recruitment of Vps34 into Beclin 1 positive puncta was blocked with the specific autophagy inhibitor spautin-1, which suppresses USP10 activity following decorin (XREF_FIG)."

reach
"Another autophagy inhibitor, spautin-1, degrades class III PI3K by inhibiting the activity of the ubiquitin specific peptidases, USP10 and USP13 (Mateo et al., 2013, Liu et al., 2011)."

reach
"First, spautin-1 is an inhibitor of USP10 (Liu et al., 2011), a deubiquitinating enzyme that is important for CFTR homeostasis (Bomberger et al., 2009)."

reach
"To directly test if spautin-1 can inhibit the deubiquitinating activity of USP10 and USP13, we tested the activity of isolated USP10 and USP13 on ubiquitinated Beclin1 in vitro."

reach
"USP10 inhibition by spautin-1 significantly offset the protective role of FSTL1 in T2DM mice that underwent MI (Figures 5A–H)."

reach
"In contrast, recruitment of Vps34 into Beclin 1 positive puncta was blocked with the specific autophagy inhibitor spautin-1, which suppresses USP10 activity following decorin (XREF_FIG)."

reach
"In addition, the inhibition of USP10 by spautin-1 significantly suppressed the migration and metastasis of HCC."

reach
"Using this assay, we found that spautin-1 inhibited USP10 and USP13 with IC 50 of ~ 0.6-0.7 microM while having no inhibitory activity toward CYLD which is also a member of ubiquitin specific peptidase family (XREF_FIG)."

reach
"Furthermore, our unpublished data could not recapitulate the inhibition of USP10 by spautin-1."

reach
"Since USP10 is known as a deubiquitinating protease of p53, inhibition of USP10 by spautin-1 may promote the degradation of p53."
| 2

eidos
"Furthermore , our unpublished data could not recapitulate the inhibition of USP10 by spautin-1 ."

eidos
"Consistent with these findings , inhibition of USP10 by Spautin-1 resulted in accumulation of CCND3 with K48-linked polyubiquitination and degradation that synergized with Palbociclib , a CDK4 / 6 inhibitor , to induce MM cell apoptosis ."
Spautin-1 decreases the amount of USP10.
| 2
Spautin-1 decreases the amount of USP10. 2 / 2
| 2

reach
"In addition, spautin-1 also downregulated the expression of USP10 and USP13 at the protein level [53]."

reach
"Interestingly, the treatment of spautin-1 also reduced the levels of USP10 and USP13, but not USP14, a DUB involved in regulating proteasome function, or Rubicon, a negative regulator of type III PI3 kinase (XREF_FIG)."
Spautin-1 binds USP10.
| 2
USP10 binds spautin-1. 2 / 2
| 2

reach
"However, there is still no evidence showing that spautin-1 or spautin-A41 directly binds to USP10."

reach
"No orthogonal biophysical methods, such as the surface plasmon response (SPR) or isothermal titration calorimetry (ITC), were used to validate the binding of spautin-1 with USP10 or USP13."
Spautin-1 activates USP10.
| 2
Spautin-1 activates USP10. 2 / 2
| 2

reach
"Briefly, spautin-1 targets two ubiquitin specific peptidases (USP10 and USP13), both of which mediate deubiquitination of Beclin1 and p53, thus leading to their proteasomal degradation [93]."

reach
"Because the reductions in the levels of USP10 and USP13 in H4-LC3-GFP cells treated with spautin-1 appeared later than the reductions in the levels of Vps34 complexes and autophagy (XREF_FIG), the reduced levels of USP10 and USP13 are unlikely to be the primary reason for the ability of spautin-1 to reduce the levels of PtdIns3P and inhibit autophagy."
USP10 affects IKBKG
4 1 | 8 4
USP10 binds IKBKG.
4 | 1 4
4 | 1

No evidence text available

No evidence text available

No evidence text available

reach
"[104] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO attached M1 linked polyubiquitin chains, thus inhibiting the genotoxic NF-kappaB signaling cascade."

No evidence text available
| 4

sparser
"NEMO ubiquitylation is decreased through the deubiquitinase USP10, which interacts with NEMO via MCPIP1 upon genotoxic stress."

sparser
"Moreover, usp4-depleted zebrafish (Danio rerio) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment. [102] Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) cleaves K63-linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to highrisk human papillomavirus (hrHPV) infection. [103] TRAF family member-associated NF-B activator (TANK) interacts with both monocyte chemotactic protein-1-induced protein-1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF-B activation in response to TLR activation. [104] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO-attached M1-linked polyubiquitin chains, thus inhibiting the genotoxic NF-B signaling cascade. [40] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."

sparser
"Moreover, usp4 ‐depleted zebrafish ( Danio rerio ) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment. [ xref ] Ubiquitin carboxyl‐terminal hydrolase L1 (UCHL1) cleaves K63‐linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to high‐risk human papillomavirus (hrHPV) infection. [ xref ] TRAF family member‐associated NF‐ κ B activator (TANK) interacts with both monocyte chemotactic protein‐1‐induced protein‐1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF‐ κ B activation in response to TLR activation. [ xref ] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO‐attached M1‐linked polyubiquitin chains, thus inhibiting the genotoxic NF‐ κ B signaling cascade. [ xref ] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."

sparser
"Moreover, usp4‐depleted zebrafish (Danio rerio) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment.[ 102 ] Ubiquitin carboxyl‐terminal hydrolase L1 (UCHL1) cleaves K63‐linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to high‐risk human papillomavirus (hrHPV) infection.[ 103 ] TRAF family member‐associated NF‐κB activator (TANK) interacts with both monocyte chemotactic protein‐1‐induced protein‐1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF‐κB activation in response to TLR activation.[ 104 ] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO‐attached M1‐linked polyubiquitin chains, thus inhibiting the genotoxic NF‐κB signaling cascade.[ 40 ] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."
USP10 deubiquitinates IKBKG.
1 | 6
USP10 deubiquitinates IKBKG. 7 / 7
1 | 6

reach
"By mediating USP10 dependent deubiquitination of NEMO, MCPIP1 serves in a negative feedback mechanism for attenuation of NF-kappaB activation [XREF_BIBR]."

reach
"One example is USP10 that requires the protein MCPIP-1 (monocyte chemotactic protein induced protein 1) to interact with and deubiquitinate its substrate NEMO inhibiting the NF-kappaB signalling cascade [XREF_BIBR]."

reach
"MCPIP1 induction serves as a negative feedback mechanism for attenuating NF-kappaB activation in genotoxic response by mediating USP10 dependent deubiquitination of NEMO [13]."

reach
"The USP10-dependent deubiquitination of NEMO and TRAF6 proteins is another strategy through which MCPIP1 can regulate inflammation and the immune response [34]."

reach
"USP10 deubiquitinates IKKgamma and NEMO, thereby inhibiting IKKgamma mediated nuclear factor kappaB (NF-kappaB) activation after genotoxic stress."

reach
"Thus, by mediating USP10 dependent deubiquitination of NEMO, MCPIP1 induction serves as a negative feedback mechanism for attenuating genotoxic NF-kappaB activation."
USP10 inhibits IKBKG.
| 1
USP10 inhibits IKBKG. 1 / 2
| 1

reach
"NEMO protein evidently interacted with USP10, whereby USP10 inhibition accelerated the degradation of NEMO."
Spautin-1 affects USP10
1 | 17
Spautin-1 inhibits USP10.
1 | 13
1 | 13

reach
"Indeed, a recent study showed that a small molecule, Spautin-1, promoted the degradation of Vps34 by inhibiting two ubiquitin specific proteases USP10 and USP13 that regulate the stability of the Vps34 complex [XREF_BIBR]."

reach
"58 Spautin-1 promotes the degradation of BECN1 by inhibiting 2 USPs (ubiquitin-specific peptidases), USP10 and USP13, which target BECN1."

reach
"A study by Liu et al. (Liu et al., 2011) has demonstrated that Spautin-1 is a potent small-molecule inhibitor of USP10 and USP13, and treatment with Spautin-1 inhibits the deubiquitinase activity of USP10 and USP13, resulting in an increasing ubiquitination and accelerating degradation of Beclin1 in Vps34 complexes, ultimately inhibiting autophagy."

reach
"Spautin-1 inhibits ubiquitin specific peptidases, USP10 and USP13, and promotes the degradation of Vps34-PI3 kinase complexes, key regulators of autophagy, leading to inhibition of autophagy."

reach
"Consistent with these findings, inhibition of USP10 by Spautin-1 resulted in accumulation of CCND3 with K48-linked polyubiquitination and degradation that synergized with Palbociclib, a CDK4/6 inhibitor, to induce MM cell apoptosis."

reach
"SBI blocks the kinase activity of ULK1, diminishing the phospho regulated capacity of beclin-1 to engage with its binding partners (Egan et al., 2015) and Spautin-1 inhibits the USP10 and USP13 hydrol[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"A previous study reported that Spautin-1 also inhibits USP10 activity XREF_BIBR."

reach
"Spautin-1 inhibits the catalytic activity of both USP10 and USP13 with an IC 50 of ~ 0.6-0.7 microM."

reach
"Molecular analysis revealed that Spautin-1 directly inhibits the activity of the deubiquitinating enzymes (DUBs) ubiquitin specific protease 10 (USP10) and USP13."

No evidence text available
Spautin-1 activates USP10.
| 4
Spautin-1 activates USP10. 4 / 4
| 4

reach
"Spautin-1 targets the activity of two ubiquitin specific peptidases, USP10 and USP13 and causes PI3K complex degradation."

reach
"Spautin-1 reportedly inhibits autophagosomal initiation and maturation by inhibiting deubiquitylases USP10 and USP13, which stabilize the Beclin-1 and VPS34 complex (26)."

reach
"Spautin-1 has been recently reported to prevent autophagy by inhibiting the deubiquitinases USP10 and USP13, which leads to beclin 1 and Vps34 (or PI3KIII) degradation [XREF_BIBR]."

reach
"Spautin-1 reportedly suppresses autophagy by inhibiting the USP10 and USP13 deubiquitinases."
SMAD4 affects USP10
| 6 12
| 6 12

reach
"Further evidence revealed that USP10 directly binds to Smad4 and stabilizes Smad4 through deubiquitination [46]."

sparser
"Therefore, these results collectively revealed the specific interaction between USP10 and Smad4 both at the endogenous and exogenous levels."

reach
"As USP10 directly binds to Smad4 and regulates its protein stability, we hypothesized that USP10 exerts such regulation through the deubiquitination of Smad4."

sparser
"Further evidence revealed that USP10 directly binds to Smad4 and stabilizes Smad4 through deubiquitination [46] ."

sparser
"As USP10 directly binds to Smad4 and regulates its protein stability, we hypothesized that USP10 exerts such regulation through the deubiquitination of Smad4."

sparser
"USP10 interacts with Smad4 in vivo ."

sparser
"USP10 directly interacts with SMAD4 and stabilizes it to promote HCC proliferation and metastasis (149) ."

reach
"Further analysis showed that USP10 directly interacts with Smad4 and stabilizes it through the cleavage of its proteolytic ubiquitination, thus promoting HCC metastasis."

reach
"More importantly, the interaction between the endogenous USP10 and endogenous Smad4 was further demonstrated in our Co‐IP analyses (Fig. 3D)."

sparser
"In addition, we also observed an interaction between the Flag‐tagged Smad4 and endogenous USP10 (Fig. xref C)."
AR affects USP10
2 | 4 8
2 | 4 8

No evidence text available

sparser
"In order to determine whether the interaction of USP10 with the AR affected its transcriptional function, a construct for transient expression was devised in the pSG5 plasmid."

sparser
"Moreover, USP10 not only binds AR, resulting in increased transcriptional activity, but also deubiquitinates the histone variant H2A.Z, both of which are required for AR − mediated gene activation [ xref , xref ]."

reach
"USP10 was originally detected in DNA bound AR complexes but the results of GST pull-down experiments indicate that USP10 may interact with the AR also in absence of response elements, at least when us[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

No evidence text available

sparser
"USP10 was originally detected in DNA-bound AR complexes but the results of GST pull-down experiments indicate that USP10 may interact with the AR also in absence of response elements, at least when us[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The difference in migration was probably due to post-translational modifications.In order to further analyse the interaction between USP10 and the AR, GST pull-down experiments were performed."

sparser
"In order to further analyse the interaction between USP10 and the AR, GST pull-down experiments were performed."

reach
"Usp10 can bind to AR and positively regulate its transcriptional activity [XREF_BIBR] however, it also regulates AR indirectly via deubiquitination of H2A.Z causing the same effect [XREF_BIBR]."

sparser
"Our data suggest that if USP10 and AR do physically interact, this interaction only occurs in a small nuclear fraction of USP10."
USP10 affects SKP2
| 15 2
USP10 binds SKP2.
| 4 2
| 4 2

reach
"By further mapping the regions of USP10, we showed that the N-terminal region (1-205aa) was required for the binding of USP10 to SKP2."

reach
"Mechanically, USP10 can bind to Skp2 and stabilize its protein level by removing polyubiquitin on Skp2 in the cytoplasm."

reach
"We also validated the interaction between exogenous USP10 and SKP2 using Co-IP in HEK293 cells that were co-transfected with FLAG-USP10, FLAG-USP13, and HA-SKP2 plasmids."

sparser
"Mechanically, USP10 can bind to Skp2 and stabilize its protein level by removing polyubiquitin on Skp2 in the cytoplasm."

sparser
"By further mapping the regions of USP10, we showed that the N-terminal region (1-205aa) was required for the binding of USP10 to SKP2 (Fig.  xref )."

reach
"We found that SKP2 interacted with USP10, USP13, UCHL5, USP14, and USP7 in KBM5-T315I cells."
USP10 increases the amount of SKP2.
| 5
USP10 increases the amount of SKP2. 3 / 3
| 3

reach
"We further explored whether USP10 modulates the expression of SKP2 and thus activating Bcr-Abl in CML cells."

reach
"XREF_FIG, only the knockdown of USP10 and USP13 decreased the expression of SKP2."

reach
"We further found that only USP10-WT, but not USP10-CA, increased the protein level of SKP2, suggesting that deubiquitinating activity of USP10 is required for SKP2 enrichment."
Modified USP10 increases the amount of SKP2. 2 / 2
| 2

reach
"The results showed that overexpression of USP10 increased the protein level of SKP2 in HEK293T and HeLa cells in a manner similar to USP13."

reach
"The result showed that loss of USP10 significantly decreased the expression of SKP2, as well as the phosphorylation and downstream signals of Bcr-Abl, suggesting that USP10 is required for the expression of SKP2 and the phosphorylation of Bcr-Abl in CML cells."
USP10 deubiquitinates SKP2.
| 3
USP10 deubiquitinates SKP2. 3 / 3
| 3

reach
"USP10 deubiquitinates and stabilizes SKP2."

reach
"Increased USP10 led to the deubiquitination and stabilization of SKP2, causing BCR-ABL activation and promoting CML cells’ proliferation [85,86]."

reach
"Additionally, Liao and colleagues [XREF_BIBR] found that USP10 can promote leukemic cell proliferation by deubiquitinating and stabilizing the S-phase kinase associated protein 2 (SKP2) that acts as a co-regulator of BCR-ABL mediating its K63 linked ubiquitination and activation in CML cells [XREF_BIBR]."
USP10 activates SKP2.
| 3
USP10 activates SKP2. 3 / 3
| 3

reach
"Notably, genetic or pharmacological inhibition of USP10 using Spautin-1 prominently decreased the stability of endogenous SKP2 protein, suggesting that USP10 inhibits the degradation of SKP2 in vivo."

reach
"USP10 modulates the SKP2 and Bcr-Abl axis via stabilizing SKP2 in chronic myeloid leukemia."

reach
"Since USP10 is a well characterized DUB in humans, exploring whether USP10 directly regulates the stability of SKP2 protein is attractive."
USP7 affects USP10
| 1 3 11
USP7 binds USP10.
| 3 10
| 3 3

sparser
"Hydroxylation of p53 upon proline 359 regulates its interaction with USP7 and USP10, and its inhibition decreases the association of p53 with USP7/USP10, increases p53 ubiquitination, and rapidly reduces p53 protein levels independently of mRNA expression."

sparser
"We were able to identify UbVs that bound USP7 or USP10 with high affinity and inhibited deubiquitination activity."

reach
"The binding of USP7 and USP10 to NHE3 was confirmed by co-immunoprecipitation."

sparser
"In summary, we have demonstrated that USP7 and USP10 physically interact and ubiquitinate NHE3."

reach
"We showed that FSK attenuated the interaction of USP7 and USP10 with NHE3, resulting in increased ubiquitination of NHE3."

reach
"NHE3 is inhibited by protein kinase A. Activation of PKA by forskolin decreased the binding of USP7 and USP10 to NHE3, while increasing ubiquitination of NHE3."
USP10 binds USP7 and NHE3. 4 / 4
| 4

sparser
"We showed that FSK attenuated the interaction of USP7 and USP10 with NHE3, resulting in increased ubiquitination of NHE3."

sparser
"NHE3 is inhibited by protein kinase A. Activation of PKA by forskolin decreased the binding of USP7 and USP10 to NHE3, while increasing ubiquitination of NHE3."

sparser
"The binding of USP7 and USP10 to NHE3 was confirmed by co-immunoprecipitation."

sparser
"To confirm that USP7 or USP10 interact with NHE3, we performed immunoprecipitation of NHE3 using P5D4 or IgG as a negative control, followed by immunoblotting for USP7 or USP10."
TP53 binds USP10 and USP7. 3 / 3
| 3

sparser
"For example, USP7 and USP10 bind to and deubiquitylate their substrate p53 to mediate its role for suppression of cell propagation upon cellular stresses by counteracting its degradation xref – xref ."

sparser
"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced under DMOG treatment ( xref A)."

sparser
"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced un[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP7 inhibits USP10.
| 1 1
USP7 inhibits USP10. 2 / 2
| 1 1

eidos
"Weisberg et al. have performed a phenotypic screen and identified compounds originally discovered for USP7 that inhibit USP10 and induce degradation of the oncogenic kinase FLT3 [ 42 ] ."

sparser
"Weisberg et al. have performed a phenotypic screen and identified compounds originally discovered for USP7 that inhibit USP10 and induce degradation of the oncogenic kinase FLT3 [ xref ]."
USP10 affects SLC9A3
| 15
USP10 decreases the amount of SLC9A3.
| 4
USP10 decreases the amount of SLC9A3. 4 / 4
| 4

reach
"Knockdown of USP7 or USP10 resulted in increased NHE3 ubiquitination and decreased NHE3 expression at the surface membrane and cellular level."

reach
"Knockdown of either USP7 or USP10 increased ubiquitination level of NHE3, demonstrating that these proteins deubiquitinate NHE3."

reach
"Knockdown of USP7 or USP10 in SK-CO15 cells increased ubiquitination levels of NHE3 by 358 ± 62% or 396 ± 45%, respectively (Figure 2C)."

reach
"Silencing of USP7 or USP10 increased the ubiquitination levels of NHE3 compared with shCon (Figure 2B, upper panel)."
USP10 binds SLC9A3.
| 3
| 3

reach
"Using an activity-based chemical screening, we identified ubiquitin specific protease-7 (USP7) and USP10 that bind NHE3."

reach
"In summary, we have demonstrated that USP7 and USP10 physically interact and ubiquitinate NHE3."

reach
"The binding of USP7 and USP10 to NHE3 was confirmed by co-immunoprecipitation."
USP10 ubiquitinates SLC9A3.
| 2
USP10 ubiquitinates SLC9A3. 2 / 2
| 2

reach
"Knockdown of either USP7 or USP10 increased ubiquitination level of NHE3, demonstrating that these proteins deubiquitinate NHE3."

reach
"In summary, we have demonstrated that USP7 and USP10 physically interact and ubiquitinate NHE3."
USP10 inhibits SLC9A3.
| 2
USP10 inhibits SLC9A3. 2 / 2
| 2

reach
"Knockdown of USP7 or USP10 increased the association of NHE3 with Rab5a, which suggested that endocytic trafficking of NHE3 to the early endosomes was enhanced."

reach
"In this study, we found that knockdown USP7 or USP10 decreased the surface membrane abundance of NHE3."
USP10 increases the amount of SLC9A3.
| 2
USP10 increases the amount of SLC9A3. 2 / 2
| 2

reach
"Knockdown of USP7 or USP10 resulted in increased NHE3 ubiquitination and decreased NHE3 expression at the surface membrane and cellular level."

reach
"Knockdown of USP7 or USP10 decreased total NHE3 cellular expression by 44.8 ± 17.4% and 59.8 ± 5.1%, respectively."
USP10 activates SLC9A3.
| 2
USP10 activates SLC9A3. 2 / 2
| 2

reach
"In addition, USP10 knockdown decreased the half-life of NHE3 by about a half from approximately 20 hours to 12 hours."

reach
"We, therefore, determined whether USP7 and USP10 modulate NHE3 internalization."
USP10 affects Neoplasms
| 5 7
USP10 inhibits Neoplasms.
| 3 3
| 3 3

eidos
"Additionally , USP10 blocks tumor formation via p53 and SIRT6-mediated degradation of c-Myc , thereby stopping cell cycle progression and cancer cell growth [ 53 ] ."
| PMC

reach
"The overexpression of USP10 can inhibit the formation of stress granules (SGs), thus restraining the development of a tumor [19,24]."

eidos
"For example , in hepatocellular carcinoma , Zhu et al. reported that USP10 promotes proliferation [ 40 ] , whereas Lu et al. found that USP10 suppresses tumor progression by inhibiting mTOR activation [ 41 ] ."

reach
"Furthermore, USP10 can also interact with and deubiquitinate PTEN, USP10 inhibition stimulates tumor growth and invasion, but this effect can be abolished by reinserting PTEN (Sun et al., 2018)."

reach
"USP10, upon ATM-dependent phosphorylation at threonine 42 and serine 337 residues, is stabilized and translocated to the nucleus to activate p53 through deubiquitinating activity, inducing tumor cell suppression [53]."
| PMC

eidos
"CAPRIN-1 enhances proliferation [ 71,72,73 ] , whereas USP10 inhibits tumor progression and invasion [ 74,75 ] ."
USP10 activates Neoplasms.
| 2 4
| 2 4

eidos
"It has been reported that USP10 mediates cancer progression via stabilizing some substrates , such as p53 , STRT6 , AMPK , FLT3 and androgen receptor [ 20-24 ] ."

reach
"In addition to USP10 controlling cell proliferation, a study from the Ouchida group revealed that USP10 promotes tumor migration or invasion."

reach
"We next found that USP10 coordinately promotes tumor progression with G3BP1."

reach
"For instance, USP10 promotes tumor metastasis through deubiquitination and stabilization of Smad4 in advanced HCC [8]."

reach
"Our studies suggest that USP10 by protecting pancreatic cancer cells from ER stress may support tumor progression."

eidos
"On the other hand , USP10 , a p53 deubiquitinase , serves as a positive regulator of p53 ; inhibition of USP10 could suppress cancer by degrading oncogenic p53 mutants ( Figure 2b ) [ 32 ] ."
PTEN affects USP10
4 | 7 4
4 | 7 1

reach
"Therefore, we conclude that USP10 interacts with PTEN and AMPKalpha, inhibits their polyubiquitylation, and as a result stabilizes PTEN and AMPKalpha.Next, we examined whether Rapamycin, a well known [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

No evidence text available

reach
"Inversely, endogenous PTEN and AMPKalpha interacted with endogenous USP10 in HCC-LM3 and HUH7 cells."

No evidence text available

No evidence text available

reach
"Mechanistically, USP10 interacts and stabilizes PTEN and AMPKalpha by inhibiting their polyubiquitylation."

reach
"We further demonstrated that USP10 directly interacted with and stabilized PTEN via deubiquitination."

reach
"Furthermore, USP10 can also interact with and deubiquitinate PTEN, USP10 inhibition stimulates tumor growth and invasion, but this effect can be abolished by reinserting PTEN (Sun et al., 2018)."

sparser
"We found using co-immununoprecipitation/immunoblotting that USP10 interacted with PTEN and reduced the K63-linked polyubiquitination of PTEN mediated by TRIM25 in non-small cell lung cancer (NSCLC) cells."

reach
"Mechanistically, USP10 interacts with and stabilizes PTEN and AMPKalpha by inhibiting their polyubiquitylation, and subsequently suppresses the activity of mTOR signaling pathway."
| 3

sparser
"Therefore, we conclude that USP10 interacts with PTEN and AMPKα, inhibits their polyubiquitylation, and as a result stabilizes PTEN and AMPKα."

sparser
"To further dissect how USP10 inhibits mTOR activity, we investigated whether the stabilization of PTEN [] and AMPKα [] by USP10 involved the physical interactions of USP10 with PTEN and AMPKα."

sparser
"Inversely, endogenous PTEN and AMPKα interacted with endogenous USP10 in HCC-LM3 and HUH7 cells (B)."
| 3 7

eidos
"31 Recently , a report suggested that USP10 can inhibit hepatic steatosis , insulin resistance , and inflammation through Sirt6 in nonalcoholic fatty liver disease ."

reach
"31 Recently, a report suggested that USP10 can inhibit hepatic steatosis, insulin resistance, and inflammation through Sirt6 in nonalcoholic fatty liver disease."

eidos
"USP10 Inhibited Inflammation and Apoptosis in Hepatocytes After H / R Treatment To validate whether USP10 directly influences inflammation and apoptosis in hepatocytes , we detected inflammation and apoptosis in H / R-treated primary hepatocytes isolated from USP10-HZ and WT mice ."

reach
"USP10 deletion also promoted inflammatory response in ischemic penumbra of cortical regions by further accelerating nuclear factor kappaB (NF-kappaB) signaling pathway."

reach
"USP10 inhibits hepatic steatosis, insulin resistance and inflammation via Sirt6."

reach
"USP10 overexpression also inhibited while USP10 siRNA enhanced the CNYP induced inhibition of inflammation in ectopic endometrial stromal cells."

reach
"Additionally, USP10 overexpression inhibited hepatocyte inflammation and apoptosis in hepatic I/R injury in vitro and in vivo."

reach
"Ubiquitin Specific Peptidase 10 (USP10) Inhibits Hepatic Steatosis, Insulin Resistance, and Inflammation Through Sirt6."

reach
"For example, USP10 expression is decreased and upregulated in hepatic steatosis models to inhibit hepatic steatosis, insulin resistance, and inflammation [7]."

eidos
"For example , USP10 expression is decreased and upregulated in hepatic steatosis models to inhibit hepatic steatosis , insulin resistance , and inflammation [ 7 ] ."
| 2 1

eidos
"USP10 could promote autophagy-related pathways and molecules and increase the synthesis of autophagosomes in NASH , improving steatosis , inflammation , and fibrosis ."

reach
"USP10 could promote autophagy-related pathways and molecules and increase the synthesis of autophagosomes in NASH, improving steatosis, inflammation, and fibrosis."

eidos
"USP10 ameliorated histological steatosis , inflammation , and fibrosis ."
USP10 affects USP7
| 3 10
| 3 3

sparser
"Hydroxylation of p53 upon proline 359 regulates its interaction with USP7 and USP10, and its inhibition decreases the association of p53 with USP7/USP10, increases p53 ubiquitination, and rapidly reduces p53 protein levels independently of mRNA expression."

sparser
"We were able to identify UbVs that bound USP7 or USP10 with high affinity and inhibited deubiquitination activity."

reach
"The binding of USP7 and USP10 to NHE3 was confirmed by co-immunoprecipitation."

sparser
"In summary, we have demonstrated that USP7 and USP10 physically interact and ubiquitinate NHE3."

reach
"We showed that FSK attenuated the interaction of USP7 and USP10 with NHE3, resulting in increased ubiquitination of NHE3."

reach
"NHE3 is inhibited by protein kinase A. Activation of PKA by forskolin decreased the binding of USP7 and USP10 to NHE3, while increasing ubiquitination of NHE3."
USP10 binds USP7 and NHE3. 4 / 4
| 4

sparser
"We showed that FSK attenuated the interaction of USP7 and USP10 with NHE3, resulting in increased ubiquitination of NHE3."

sparser
"NHE3 is inhibited by protein kinase A. Activation of PKA by forskolin decreased the binding of USP7 and USP10 to NHE3, while increasing ubiquitination of NHE3."

sparser
"The binding of USP7 and USP10 to NHE3 was confirmed by co-immunoprecipitation."

sparser
"To confirm that USP7 or USP10 interact with NHE3, we performed immunoprecipitation of NHE3 using P5D4 or IgG as a negative control, followed by immunoblotting for USP7 or USP10."
TP53 binds USP10 and USP7. 3 / 3
| 3

sparser
"For example, USP7 and USP10 bind to and deubiquitylate their substrate p53 to mediate its role for suppression of cell propagation upon cellular stresses by counteracting its degradation xref – xref ."

sparser
"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced under DMOG treatment ( xref A)."

sparser
"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced un[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP10 affects CKO
| 13
| 13

sparser
"USP10-CKO mice were constructed by Shanghai Model Organisms through CRISPR-Cas9 technology."

sparser
"Western blot analysis demonstrated a specific Sirt6 decrease in the hearts of USP10CKO mice 4 weeks after AB surgery, whereas Sirt6 was upregulated when USP10 was overexpressed (Figure  xref and xref )."

sparser
"Cardiac‐specific USP10 knockout (USP10CKO) mice and USP10‐transgenic (USP10‐TG) mice were used to examined the role of USP10 in CH following aortic banding."

sparser
"Moreover, hematoxylin and eosin and Picrosirius red staining of heart sections indicated that compared with those of their USP10‐Flox littermates, hearts from USP10CKO mice developed significant cardiomyocyte hypertrophy and fibrosis in the interstitial and perivascular spaces (Figure  xref through xref )."

sparser
"Furthermore, the USP10CKO mice exhibited significantly increased LVEDd and LVESd values and decreased fractional shortening% and ejection fraction values compared with USP10‐Flox controls (Figure  xref through xref )."

sparser
"Subsequently, USP10‐Flox and USP10CKO mice were subjected to AB surgery, and cardiac parameters were evaluated."

sparser
"In addition, myocardial fibrosis in perivascular and interstitial spaces were significantly increased in USP10-CKO mice ( xref )."

sparser
"Western blotting results demonstrated that phosphorylation levels of Akt, GSK3β, mTOR, and p70‐S6K were significantly increased in hypertrophic hearts in USP10CKO mice compared with those in USP10‐Flox controls in response to AB surgery (Figure  xref )."

sparser
"As shown in Figure  xref , the ratios of heart weight (HW) to body weight (BW) increased in USP10CKO mice compared with USP10‐Flox group at 4 weeks after AB surgery (Figure  xref )."

sparser
"First, we tested Sirt6 level in USP10CKO and USP10‐TG mice."
G3BP1 affects CAPRIN1
| 13
| 7

sparser
"However, a recent study suggests that G3BP proteins interact with Caprin1 and USP10 but that they are mutually exclusive allowing the binding of these proteins to serve as a switch for SG formation depending on the binding partner interacting with G3BP1."

sparser
"However, it is important to note that the interactions between G3BP1 and USP10 or Caprin1 do not fully dictate SG disassembly or assembly, as two different laboratories recently demonstrated that the unstressed G3BP1 interactome significantly overlaps the interactome of G3BP1 during stress [ xref , xref ]."

sparser
"USP10 and Caprin1 antagonistically bind G3BP to regulate SG formation."

sparser
"Our results indicated that N expression abrogates G3BP1 interaction with the SG proteins CAPRIN1 and USP10 ( Figure 2C ), reinforcing the idea that N inhibits the formation of SGs in host cells by sequestering G3BPs away from their interacting SG-nucleating proteins."

sparser
"Rescue experiments using G3BP1 mutants show that phosphomimetic G3BP1-S149E fails to rescue SG formation, whereas G3BP1-F33W, a mutant unable to bind G3BP partner proteins Caprin1 or USP10, rescues SG formation."

sparser
"Therefore, although Caprin1 and USP10 both bind G3BP ( xref ; xref ), they have antagonistic effects on SGs."

sparser
"Interaction of Dengue RNA with stress granule proteins G3BP1, USP10 and caprin1 occurs at an adjacent unstructured region of the 3′ UTR ( xref )."
| 6

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [ xref ], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [ xref ]."

sparser
"However, a role for the G3BP1 and G3BP2 interactions with CAPRIN1 and/or USP10 under chronic nutrient starvation has not yet been investigated."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al.2017); SeV Trailer RNA captures TIAR from SG (Iseni et al.2002); West Nile Virus (WNV) and Dengue virus (DENV) 3′-end viral genome captures TIA-1/TIAR (Li et al.2002; Emara and Brinton 2007; Xia et al.2015); DENV 3′-UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al.2011; Reineke et al.2015); JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al.2011)."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al. 2017) ; SeV Trailer RNA captures TIAR from SG (Iseni et al. 2002) ; West Nile Virus (WNV) and Dengue virus (DENV) 3 0 -end viral genome captures TIA-1/TIAR (Li et al. 2002; Emara and Brinton 2007; Xia et al. 2015) ; DENV 3 0 -UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al. 2011; Reineke et al. 2015) ; JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al. 2011) ."

sparser
"Additional experiments demonstrate that, in contrast to DDX6, the SG proteins G3BP1, G3BP2, Caprin1 and USP10 bind to the variable region (VR) in the 3' UTR."

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [171], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [172]."
CKO affects USP10
| 13
| 13

sparser
"USP10-CKO mice were constructed by Shanghai Model Organisms through CRISPR-Cas9 technology."

sparser
"Western blot analysis demonstrated a specific Sirt6 decrease in the hearts of USP10CKO mice 4 weeks after AB surgery, whereas Sirt6 was upregulated when USP10 was overexpressed (Figure  xref and xref )."

sparser
"Cardiac‐specific USP10 knockout (USP10CKO) mice and USP10‐transgenic (USP10‐TG) mice were used to examined the role of USP10 in CH following aortic banding."

sparser
"Moreover, hematoxylin and eosin and Picrosirius red staining of heart sections indicated that compared with those of their USP10‐Flox littermates, hearts from USP10CKO mice developed significant cardiomyocyte hypertrophy and fibrosis in the interstitial and perivascular spaces (Figure  xref through xref )."

sparser
"Furthermore, the USP10CKO mice exhibited significantly increased LVEDd and LVESd values and decreased fractional shortening% and ejection fraction values compared with USP10‐Flox controls (Figure  xref through xref )."

sparser
"Subsequently, USP10‐Flox and USP10CKO mice were subjected to AB surgery, and cardiac parameters were evaluated."

sparser
"In addition, myocardial fibrosis in perivascular and interstitial spaces were significantly increased in USP10-CKO mice ( xref )."

sparser
"Western blotting results demonstrated that phosphorylation levels of Akt, GSK3β, mTOR, and p70‐S6K were significantly increased in hypertrophic hearts in USP10CKO mice compared with those in USP10‐Flox controls in response to AB surgery (Figure  xref )."

sparser
"As shown in Figure  xref , the ratios of heart weight (HW) to body weight (BW) increased in USP10CKO mice compared with USP10‐Flox group at 4 weeks after AB surgery (Figure  xref )."

sparser
"First, we tested Sirt6 level in USP10CKO and USP10‐TG mice."
CAPRIN1 affects G3BP1
| 13
| 7

sparser
"However, a recent study suggests that G3BP proteins interact with Caprin1 and USP10 but that they are mutually exclusive allowing the binding of these proteins to serve as a switch for SG formation depending on the binding partner interacting with G3BP1."

sparser
"However, it is important to note that the interactions between G3BP1 and USP10 or Caprin1 do not fully dictate SG disassembly or assembly, as two different laboratories recently demonstrated that the unstressed G3BP1 interactome significantly overlaps the interactome of G3BP1 during stress [ xref , xref ]."

sparser
"USP10 and Caprin1 antagonistically bind G3BP to regulate SG formation."

sparser
"Our results indicated that N expression abrogates G3BP1 interaction with the SG proteins CAPRIN1 and USP10 ( Figure 2C ), reinforcing the idea that N inhibits the formation of SGs in host cells by sequestering G3BPs away from their interacting SG-nucleating proteins."

sparser
"Rescue experiments using G3BP1 mutants show that phosphomimetic G3BP1-S149E fails to rescue SG formation, whereas G3BP1-F33W, a mutant unable to bind G3BP partner proteins Caprin1 or USP10, rescues SG formation."

sparser
"Therefore, although Caprin1 and USP10 both bind G3BP ( xref ; xref ), they have antagonistic effects on SGs."

sparser
"Interaction of Dengue RNA with stress granule proteins G3BP1, USP10 and caprin1 occurs at an adjacent unstructured region of the 3′ UTR ( xref )."
| 6

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [ xref ], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [ xref ]."

sparser
"However, a role for the G3BP1 and G3BP2 interactions with CAPRIN1 and/or USP10 under chronic nutrient starvation has not yet been investigated."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al.2017); SeV Trailer RNA captures TIAR from SG (Iseni et al.2002); West Nile Virus (WNV) and Dengue virus (DENV) 3′-end viral genome captures TIA-1/TIAR (Li et al.2002; Emara and Brinton 2007; Xia et al.2015); DENV 3′-UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al.2011; Reineke et al.2015); JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al.2011)."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al. 2017) ; SeV Trailer RNA captures TIAR from SG (Iseni et al. 2002) ; West Nile Virus (WNV) and Dengue virus (DENV) 3 0 -end viral genome captures TIA-1/TIAR (Li et al. 2002; Emara and Brinton 2007; Xia et al. 2015) ; DENV 3 0 -UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al. 2011; Reineke et al. 2015) ; JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al. 2011) ."

sparser
"Additional experiments demonstrate that, in contrast to DDX6, the SG proteins G3BP1, G3BP2, Caprin1 and USP10 bind to the variable region (VR) in the 3' UTR."

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [171], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [172]."
USP10 affects USP13
| 4 5
| 4 3

sparser
"USP13 also interacts with USP10, and the interaction increases USP13 deubiquitinating activity ( xref )."

reach
"Although no structural information for USP13 and USP10 is currently available, the enhanced DUB activity when USP13 interacts with USP10 or when they interact with Beclin1 suggest that the interaction of USP13 and USP10 with each other or with their substrates can lead to changes in their conformation which may be critical for the catalytic activities."

reach
"Using spautin-1 as a tool, we explored the interaction of USP10 and USP13 with Vps34 complexes."

reach
"USP13 also interacts with USP10, and the interaction increases USP13 deubiquitinating activity."

sparser
"Although no structural information for USP13 and USP10 is currently available, the enhanced DUB activity when USP13 interacts with USP10 or when they interact with Beclin1 suggest that the interaction of USP13 and USP10 with each other or with their substrates can lead to changes in their conformation which may be critical for the catalytic activities."

reach
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."

sparser
"No orthogonal biophysical methods, such as the surface plasmon response (SPR) or isothermal titration calorimetry (ITC), were used to validate the binding of spautin-1 with USP10 or USP13."
| 2

sparser
"To determine the mechanism by which Vps34 complexes regulate the stability of USP13 and USP10, we examined the possibility that Beclin1 may interact with USP10 and USP13."

sparser
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."
USP10 affects NFE2L2
| 2 9 1
USP10 activates NFE2L2.
| 2 5 1
USP10 activates NFE2L2. 8 / 8
| 2 5 1

sparser
"These results suggest that PERK is not involved in the Nrf2 activation by USP10 in dopamine-treated SH-SY5Y cells."

reach
"Finally, we discovered that USP10 promoted activation of the NRF2/HO-1 pathway through SIRT6 and attenuated LPS-induced renal tubular epithelial cell injury."

eidos
"Our study revealed that USP10 attenuated sepsis-induced oxidative stress in kidney tissues and activated the NRF2 / ARE signaling pathway ."

reach
"USP10 promotes activation of the NRF2/HO-1 pathway through SIRT6 and attenuates LPS-induced renal tubular epithelial cell injury."

reach
"This study found that USP10 activates the NRF2 and ARE signaling through SIRT6."

eidos
"Conclusions This study found that USP10 activates the NRF2 / ARE signaling through SIRT6 ."

reach
"This study found that USP10 activates the NRF2/ARE signaling through SIRT6."

reach
"Collectively, these results suggest that USP10 promotes activation of the NRF2/HO-1 pathway through SIRT6 and attenuates LPS-induced renal tubular epithelial cell injury."
USP10 inhibits NFE2L2.
| 2
USP10 inhibits NFE2L2. 2 / 2
| 2

reach
"Our study revealed that USP10 attenuated sepsis-induced oxidative stress in kidney tissues and activated the NRF2/ARE signaling pathway."

reach
"Moreover, USP10 attenuates sepsis-induced oxidative stress in renal tissues by increasing SIRT6 stability and activating the NRF2 /ARE signaling pathway."
USP10 increases the amount of NFE2L2.
| 2
USP10 increases the amount of NFE2L2. 2 / 2
| 2

reach
"Western blot assay showed that LPS resulted in decreased Sirt6 and increased Nrf2 and HO-1 levels, whereas USP10 overexpression led to an increased expression of Sirt6, Nrf2 and HO-1 in HK-2 cells induced by LPS (Fig. 6 A)."

reach
"Furthermore, our results demonstrated that USP10 overexpression increased Sirt6, Nrf2 and HO-1 levels, but were reduced following Sirt6 knockdown (Fig. 6B)."
USP10 affects FAM126A
| 12
| 10

reach
"The suppression of USP10 by either shRNAs or catalytic inhibitor Spautin-1 significantly inhibited the migration of HCC cells, whereas the reconstitution of Smad4 was able to efficiently rescue this defect."

reach
"In addition, the inhibition of USP10 by spautin-1 significantly suppressed the migration and metastasis of HCC."

reach
"As shown in Fig. S5D, the protein levels of USP10 were more abundant and highly correlated with Smad4 expression in tumorous regions compared with that in nontumorous tissues.These results demonstrate that USP10 reinforces the TGF‐β signaling and promotes the metastasis of HCC cells."

reach
"Taken together, these data suggested that depletion of USP10 significantly inhibits HCC metastasis both in vitro and in vivo."

reach
"To extend our in vitro observations concerning USP10’s role in promoting HCC metastasis, we evaluated whether USP10 could promote HCC metastasis in vivo."

reach
"Moreover, ablation of USP10 by either shRNAs or USP10 inhibitor Spautin‐1 significantly suppresses the metastasis of HCC cells in vitro and/or in vivo, whereas the reconstitution of Smad4 was able to efficiently rescue this defect, indicating that targeting USP10 could be a novel therapeutic strategy of metastatic HCC displaying high level of Smad4.2 Materials and methods."

reach
"The current study identified the prometastatic roles of USP10 in HCC and found that USP10 promotes TGF‐β signaling and HCC metastasis by stabilizing Smad4 through the cleavage of proteolytic K48‐linked ubiquitination.5 Conclusions."

reach
"In order to demonstrate that Smad4 indeed involved in the regulation of USP10 promoting HCC metastasis, we investigated whether Smad4 could rescue the metastasis defect caused by USP10 depletion."

reach
"Further study demonstrated that USP10 promotes TGF‐β signaling and HCC metastasis through the regulation on the protein abundance of Smad4, but imposing minimal effect on Smad2 and Smad3."

reach
"Furthermore, we demonstrate that depletion of USP10 or depriving of its catalytic activity with small‐molecule inhibitor Spautin‐1 significantly represses the metastasis of HCC cells in vitro and/or in vivo, whereas the reconstitution of Smad4 was able to efficiently rescue this defect."
USP10 bound to it activates FAM126A. 2 / 2
| 2

reach
"USP10 directly interacts with SMAD4 and stabilizes it to promote HCC proliferation and metastasis (149)."

reach
"Further analysis showed that USP10 directly interacts with Smad4 and stabilizes it through the cleavage of its proteolytic ubiquitination, thus promoting HCC metastasis."
AMPK_alpha affects USP10
| 7 5
| 7 2

reach
"USP10 interacts with AMPK and deubiquitinates AMPKalpha."

reach
"As result, AMPKalpha interacts with USP10 and significantly inhibits polyubiquitylation of AMPKalpha in Hela cells."

reach
"Mechanistically, USP10 interacts and stabilizes PTEN and AMPKalpha by inhibiting their polyubiquitylation."

sparser
"As result, AMPKα interacts with USP10 and significantly inhibits polyubiquitylation of AMPKα in Hela cells ()."

sparser
"As USP10 binds to AMPKα and regulates AMPK activation, we hypothesized that USP10 might regulate AMPK activation through the deubiquitination of AMPKα."

reach
"Mechanistically, USP10 interacts with and stabilizes PTEN and AMPKalpha by inhibiting their polyubiquitylation, and subsequently suppresses the activity of mTOR signaling pathway."

reach
"Inversely, endogenous PTEN and AMPKalpha interacted with endogenous USP10 in HCC-LM3 and HUH7 cells."

reach
"Therefore, we conclude that USP10 interacts with PTEN and AMPKalpha, inhibits their polyubiquitylation, and as a result stabilizes PTEN and AMPKalpha.Next, we examined whether Rapamycin, a well known [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"As USP10 binds to AMPKalpha and regulates AMPK activation, we hypothesized that USP10 might regulate AMPK activation through the deubiquitination of AMPKalpha."
| 3

sparser
"Therefore, we conclude that USP10 interacts with PTEN and AMPKα, inhibits their polyubiquitylation, and as a result stabilizes PTEN and AMPKα."

sparser
"To further dissect how USP10 inhibits mTOR activity, we investigated whether the stabilization of PTEN [] and AMPKα [] by USP10 involved the physical interactions of USP10 with PTEN and AMPKα."

sparser
"Inversely, endogenous PTEN and AMPKα interacted with endogenous USP10 in HCC-LM3 and HUH7 cells (B)."
USP10 affects TANK
2 | 4 5
2 | 1 3

No evidence text available

sparser
"In the case of TANK, a TANK-monocyte chemotactic protein-induced protein-1 (MCPIP1)-USP10 complex operates, with TANK playing an adaptor role for TRAF6 deubiquitination by USP10."

reach
"Intriguingly, TANK was identified as a MCPIP1 interacting protein in a proteomic screen and we confirmed that TANK forms a complex with MCPIP1 and USP10."

sparser
"MCPIP1 forms a complex with USP10 and TANK, which mediates the deubiquitination of the TRAF6 K63 chain [76] and inhibits NF-κB activation upon DNA damage [33] ."

sparser
"By forming a complex with the TANK and USP10 proteins, MCPIP1 plays an indirect role in the deubiquitination of TRAF6."

No evidence text available
| 3 2

reach
"Genotoxic stress also produced a TANK, MCPIP1, and USP10 complex that effected and enhanced deubiquitination of both TRAF6 (E3 Ubiquitin Protein Ligase) and NEMO, which in turn inhibited NF-kappaB activation."

sparser
"MCPIP1 forms a complex with USP10 and TANK, which mediates the deubiquitination of the TRAF6 K63 chain [76] and inhibits NF-κB activation upon DNA damage [33] ."

sparser
"Intriguingly, TANK was identified as a MCPIP1-interacting protein in a proteomic screen and we confirmed that TANK forms a complex with MCPIP1 and USP10."

reach
"Furthermore, we found that the TANK, MCPIP1, and USP10 complex also decreased TRAF6 ubiquitination in cells treated with IL-1beta or LPS."

reach
"In addition to genotoxic stress, the TANK, MCPIP1, and USP10 complex is also responsible for restraining TRAF6 ubiquitination in cells treated with LPS or IL-1beta."
USP10 affects RNF168
3 | 2 6
3 | 2 4

sparser
"Finally, we show that the deubiquitylase USP10 interacts with RNF168 and TOP2α, and restrains ubiquitylation of TOP2α as well as its chromatin binding."

sparser
"Therefore, interactions between USP10, RNF168, and TOP2α may play a key role in the development of tumor resistance to TOP2 inhibitors ( xref )."

No evidence text available

No evidence text available

reach
"These novel functional interactions between RNF168, TOP2alpha and USP10 are likely to contribute to the maintenance of chromosomal integrity."

reach
"Finally, we show that the deubiquitylase USP10 interacts with RNF168 and TOP2alpha, and restrains ubiquitylation of TOP2alpha as well as its chromatin binding."

No evidence text available

sparser
"Indeed, IP/IB analysis confirmed interaction of USP10 with RNF168 ( xref )."

sparser
"USP10 interacts with RNF168 and TOP2α to inhibit ubiquitination and chromatin binding of TOP2α [59] ."
USP10 binds RNF168 and TOP2α. 2 / 2
| 2

sparser
"These novel functional interactions between RNF168, TOP2α and USP10 are likely to contribute to the maintenance of chromosomal integrity."

sparser
"Given that our mass spectrometry analysis identified USP10 as a possible RNF168-interacting partner ( xref ), we hypothesized that USP10 and RNF168 forms a complex with TOP2α to co-regulate its ubiquitylation level."
| 4 5

eidos
"For example , USP10 inhibits lung cancer cell growth and invasion ."

reach
"USP10 inhibits lung cancer cell growth and invasion by upregulating PTEN."

eidos
"CAPRIN-1 enhances proliferation [ 71,72,73 ] , whereas USP10 inhibits tumor progression and invasion [ 74,75 ] ."

reach
"For example, USP10 inhibits lung cancer cell growth and invasion."

reach
"Furthermore, USP10 can also interact with and deubiquitinate PTEN, USP10 inhibition stimulates tumor growth and invasion, but this effect can be abolished by reinserting PTEN (Sun et al., 2018)."

eidos
"Furthermore , USP10 can also interact with and deubiquitinate PTEN , USP10 inhibition stimulates tumor growth and invasion , but this effect can be abolished by reinserting PTEN ( Sun et al ., 2018 ) ."

reach
"USP10 suppresses lung cancer cell invasion and growth via upregulating PTEN [22]."

eidos
"However , in lung cancer , USP10 can inhibit cell growth and invasion by stabilizing PTEN , suggesting that the roles of USP10 in the different cancer types are distinct [ 51 ] ."

reach
"Moreover, USP10 inhibited cell growth and invasion in lung cancer [XREF_BIBR], and was an independent factor for the prognosis of gastric carcinoma [XREF_BIBR]."
| 2

reach
"In addition to USP10 controlling cell proliferation, a study from the Ouchida group revealed that USP10 promotes tumor migration or invasion."

reach
"In the current study, we found that there was no correlation between expression of TM4SF1 and USP10, S100A12, p53, or Ki67 expression in GC, suggesting that TM4SF1 plays a distinct role in GC invasion and metastasis from those of USP10, S100A12, p53, or Ki67, although they all contribute to cancer invasion and metastasis."
TANK affects USP10
2 | 4 5
2 | 1 3

No evidence text available

sparser
"In the case of TANK, a TANK-monocyte chemotactic protein-induced protein-1 (MCPIP1)-USP10 complex operates, with TANK playing an adaptor role for TRAF6 deubiquitination by USP10."

reach
"Intriguingly, TANK was identified as a MCPIP1 interacting protein in a proteomic screen and we confirmed that TANK forms a complex with MCPIP1 and USP10."

sparser
"MCPIP1 forms a complex with USP10 and TANK, which mediates the deubiquitination of the TRAF6 K63 chain [76] and inhibits NF-κB activation upon DNA damage [33] ."

sparser
"By forming a complex with the TANK and USP10 proteins, MCPIP1 plays an indirect role in the deubiquitination of TRAF6."

No evidence text available
| 3 2

reach
"Genotoxic stress also produced a TANK, MCPIP1, and USP10 complex that effected and enhanced deubiquitination of both TRAF6 (E3 Ubiquitin Protein Ligase) and NEMO, which in turn inhibited NF-kappaB activation."

sparser
"MCPIP1 forms a complex with USP10 and TANK, which mediates the deubiquitination of the TRAF6 K63 chain [76] and inhibits NF-κB activation upon DNA damage [33] ."

sparser
"Intriguingly, TANK was identified as a MCPIP1-interacting protein in a proteomic screen and we confirmed that TANK forms a complex with MCPIP1 and USP10."

reach
"Furthermore, we found that the TANK, MCPIP1, and USP10 complex also decreased TRAF6 ubiquitination in cells treated with IL-1beta or LPS."

reach
"In addition to genotoxic stress, the TANK, MCPIP1, and USP10 complex is also responsible for restraining TRAF6 ubiquitination in cells treated with LPS or IL-1beta."
RNF168 affects USP10
3 | 2 6
3 | 2 4

sparser
"Finally, we show that the deubiquitylase USP10 interacts with RNF168 and TOP2α, and restrains ubiquitylation of TOP2α as well as its chromatin binding."

sparser
"Therefore, interactions between USP10, RNF168, and TOP2α may play a key role in the development of tumor resistance to TOP2 inhibitors ( xref )."

No evidence text available

No evidence text available

reach
"These novel functional interactions between RNF168, TOP2alpha and USP10 are likely to contribute to the maintenance of chromosomal integrity."

reach
"Finally, we show that the deubiquitylase USP10 interacts with RNF168 and TOP2alpha, and restrains ubiquitylation of TOP2alpha as well as its chromatin binding."

No evidence text available

sparser
"Indeed, IP/IB analysis confirmed interaction of USP10 with RNF168 ( xref )."

sparser
"USP10 interacts with RNF168 and TOP2α to inhibit ubiquitination and chromatin binding of TOP2α [59] ."
USP10 binds RNF168 and TOP2α. 2 / 2
| 2

sparser
"These novel functional interactions between RNF168, TOP2α and USP10 are likely to contribute to the maintenance of chromosomal integrity."

sparser
"Given that our mass spectrometry analysis identified USP10 as a possible RNF168-interacting partner ( xref ), we hypothesized that USP10 and RNF168 forms a complex with TOP2α to co-regulate its ubiquitylation level."
BECN1 affects USP10
| 7 4
BECN1 binds USP10.
| 3 4
| 3 2

sparser
"On the other hand, the interaction of Beclin1 and USP10 is consistent with that of enzyme/substrate which is expected to be weak and transient in nature."

reach
"On the other hand, the interaction of Beclin1 and USP10 is consistent with that of enzyme and substrate which is expected to be weak and transient in nature."

sparser
"However, the interaction of Beclin1 and USP10 was considerably weaker (data not shown)."

reach
"However, the interaction of Beclin1 and USP10 was considerably weaker (data not shown)."

reach
"To determine the mechanism by which Vps34 complexes regulate the stability of USP13 and USP10, we examined the possibility that Beclin1 may interact with USP10 and USP13."
| 2

sparser
"To determine the mechanism by which Vps34 complexes regulate the stability of USP13 and USP10, we examined the possibility that Beclin1 may interact with USP10 and USP13."

sparser
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."
BECN1 activates USP10.
| 4
BECN1 activates USP10. 4 / 4
| 4

reach
"USP10 is a de-ubiquitinating enzyme that promotes accumulation of p53, so Beclin1 mediates down-regulation of USP10 and reduces p53 levels [XREF_BIBR]."

reach
"Taken together, these results suggest that Beclin1 is the primary target of USP10 and USP13."

reach
"Another interesting observation made in the Spautin-1 study is that knock-down of VPS34 and Beclin 1 causes instability of USP10 and USP13, which indicates the existence of a regulatory feedback loop [XREF_BIBR]."

reach
"The close functional interconnection between the central PI3K-III subunits and the DUBs is demonstrated by the interesting observation that knock-down of VPS34 and Beclin 1 causes instability of USP10 and USP13, which strongly indicates the existence of a regulatory feedback loop [XREF_BIBR]."
Quercetin affects USP10
| 8 1
Quercetin activates USP10.
| 5
| 5

reach
"However, Quercetin could also target USP10 for degradation in a proteasome pathway, but not affect GATA-3 level."

reach
"The enhancement of T-bet was inhibited under Quercetin treatment because of the degradation of USP10 by Quercetin."

reach
"We want to know whether Quercetin will modulate USP10."

reach
"We further confirmed Quercetin, a reported inhibitor of T-bet, could target USP10."

reach
"Quercetin may target USP10 for degradation and then lead to the destabilization of T-bet."
Quercetin inhibits USP10.
| 1 1
| 1 1

sparser
"However, the mechanism of how quercetin inhibits USP10 remains unclear."

reach
"Quercetin treatment downregulated USP10 and promoted T-bet degradation in a proteasome dependent way."
Quercetin decreases the amount of USP10.
| 2
Quercetin decreases the amount of USP10. 2 / 2
| 2

reach
"A previous study by Pan et al. revealed that quercetin reduced the expression of USP10, which interacts with and maintains the level of T-bet, resulting in T-bet downregulation [14]."

reach
"In Human CD4 + T cells, Quercetin treatment also decreased endogenous T-bet and USP10 expression but not GATA-3."
USP10 affects Tax
| 3 7
| 3 7

sparser
"We found that interaction of Tax with USP10 inhibits arsenic-induced SG formation, stimulates ROS production, and augments ROS-dependent apoptosis in HTLV-1-infected T cells."

sparser
"In adult T-cell leukemia (AML), previous studies have revealed that the human T-cell leukemia virus type 1 (HTLV-1) oncoprotein Tax interacts with USP10 and promotes ROS-dependent apoptosis and the occurrence of AML."

sparser
"Tax also interacts with USP10, inhibiting SG assembly and enhancing the production of reactive oxygen species [227]."

sparser
"The interaction between Tax and the deubiquitinase USP10 is one of the molecular mechanisms that leads to Tax-mediated induction of ROS [ xref ]."

sparser
"Tax also interacts with the deubiquitinase USP10 to stimulate ROS production, which can contribute to Tax driven-senescence in HTLV-1-infected T cells [ xref ]."

reach
"Tax also interacts with USP10, inhibiting SG assembly and enhancing the production of reactive oxygen species [XREF_BIBR]."

sparser
"Tax mutants and USP10-knockdown indicated that Tax inhibits arsenic-induced SG formation, stimulates ROS production and augments ROS-dependent apoptosis, and they are all dependent on interactions of Tax with USP10."
| PMC

reach
"Tax also interacts with the deubiquitinase USP10 to stimulate ROS production, which can contribute to Tax driven-senescence in HTLV-1-infected T cells [XREF_BIBR]."

sparser
"Tax also interacts with USP10, inhibiting SG assembly and enhancing the production of reactive oxygen species [ xref ]."

reach
"A curved arrowhead with a black color indicates positive regulation.In addition to USP10 being regulated by the proteins of host cells, previous studies of the Takahashi group showed that HTLV-1 Tax interacted with amino acids 727–798 at the C-terminal of USP10 and inhibited USP10’s deubiquitination activity and function."
USP10 affects SYK
| 6 4
USP10 binds SYK.
| 3 4
| 3 4

reach
"To determine whether the USP10 could interact with SYK directly, we performed an in vitro binding assay with purified recombinant His USP10 and GST-SYK proteins."

sparser
"Purified His-USP10 was able to interact with GST-SYK under cell-free conditions, suggesting a direct interaction between USP10 and SYK (Fig.  xref and Supplementary Fig.  xref )."

reach
"We demonstrate a potential physical association between SYK and USP10, as both proteins co-immunoprecipitate in Ba/F3-SYK-TEL cells (a Fes immunoprecipitation was included as a control for nonspecific binding)."

sparser
"USP10 can directly interact with SYK [62] ."

reach
"Purified His USP10 was able to interact with GST-SYK under cell-free conditions, suggesting a direct interaction between USP10 and SYK."

sparser
"To investigate whether SYK and USP10 physically associate in a protein complex, we performed co-immunoprecipitation experiments."

sparser
"To determine whether the USP10 could interact with SYK directly, we performed an in vitro binding assay with purified recombinant His-USP10 and GST-SYK proteins."
USP10 activates SYK.
| 3
USP10 activates SYK. 3 / 3
| 3

reach
"14 Yang et al. provide compelling evidence that SYK can be degraded by inhibition of USP10 leading to cell death in AML cells driven by activated SYK or mutant FLT3."

reach
"The use of USP10 inhibition to degrade SYK may additionally be explored as a potential treatment approach for other malignancies for which SYK plays an important role."

reach
"Inhibition of the deubiquitinase USP10 induces degradation of SYK."
USP10 affects NLRP7
| 1 6 3
USP10 binds NLRP7.
| 3 3
| 3 3

reach
"Furthermore, we found that NLRP7 in CRC cells interacted with ubiquitin specific protease 10 (USP10), which mediated its deubiquitination, thereby increasing its protein stability."

sparser
"Binding of endogenous USP10 to NLRP7 was validated by IP assay with antibody against USP10 (Fig. xref b, lower)."

reach
"Binding of endogenous USP10 to NLRP7 was validated by IP assay with antibody against USP10."

reach
"USP10 interacts with NLRP7 and is highly expressed in CRC."

sparser
"NLRP7 interacts with USP10 to catalyze deubiquitination in colorectal cancer (CRC) cells."

sparser
"USP10 interacts with NLRP7 and is highly expressed in CRC."
USP10 deubiquitinates NLRP7.
| 1 3
USP10 deubiquitinates NLRP7. 4 / 4
| 1 3

reach
"Collectively, the present results indicated that NLRP7 in CRC cells interacts with and is deubiquitinated by USP10, resulting in increased NLRP7 protein stability."

reach
"NLRP7 deubiquitination by USP10 promotes tumor progression and tumor associated macrophage polarization in colorectal cancer."

reach
"Co-IP experiments showed that USP10 knockdown increased the ubiquitination of NLRP7 in HCT116 cells."

trips
"NLRP7 deubiquitination by USP10 promotes tumor progression and tumor-associated macrophage polarization in colorectal cancer."
USP10 affects MAPT
| 1 4 5
USP10 binds MAPT.
| 3 5
| 3 5

sparser
"Inhibition of USP10-Tau interaction might be therapeutically useful in the management of AD and related tauopathies."

reach
"Results from mass spectrometry, reciprocal immunoprecipitation, and immunofluorescence assays strongly prove Tau's interaction with USP10."

reach
"Moreover, in primary neuronal cultures, Aβ 42 induces a dose-dependent USP10 upregulation, an increase in the levels of both total and phosphorylated Tau, as well as a markedly elevated Tau binding with USP10, that is accompanied by a significantly decreased Tau ubiquitination."

sparser
"Results from mass spectrometry, reciprocal immunoprecipitation, and immunofluorescence assays strongly prove Tau's interaction with USP10."

sparser
"This is further supported by the Tau307-326K and Tau341-378K peptides' competitive inhibition of Tau binding with USP10, attenuating Tau hyperphosphorylation and Tau deubiquitination."

reach
"This is further supported by the Tau307-326K and Tau341-378K peptides' competitive inhibition of Tau binding with USP10, attenuating Tau hyperphosphorylation and Tau deubiquitination."

sparser
"Moreover, in primary neuronal cultures, Aβ 42 induces a dose-dependent USP10 upregulation, an increase in the levels of both total and phosphorylated Tau, as well as a markedly elevated Tau binding with USP10, that is accompanied by a significantly decreased Tau ubiquitination."

sparser
"The association of ubiquitin with neurofibrillary tangles is also well documented, xref in line with shreds of evidence related to Ubiquitin‐specific protease 10 (USP10) as a crucial factor for the formation of SGs comprising tau, TIA‐1, and USP10. xref USP10 also colocalizes with aggregated tau in AD patients' brain lesions, xref suggesting its role in SG mediated tau aggregation."
USP10 deubiquitinates MAPT.
| 1 1
USP10 deubiquitinates MAPT. 2 / 2
| 1 1

reach
"USP10 deubiquitinates Tau, mediating its aggregation."

trips
"USP10 deubiquitinates Tau, mediating its aggregation."
Tax affects USP10
| 3 7
| 3 7

sparser
"We found that interaction of Tax with USP10 inhibits arsenic-induced SG formation, stimulates ROS production, and augments ROS-dependent apoptosis in HTLV-1-infected T cells."

sparser
"In adult T-cell leukemia (AML), previous studies have revealed that the human T-cell leukemia virus type 1 (HTLV-1) oncoprotein Tax interacts with USP10 and promotes ROS-dependent apoptosis and the occurrence of AML."

sparser
"Tax also interacts with USP10, inhibiting SG assembly and enhancing the production of reactive oxygen species [227]."

sparser
"The interaction between Tax and the deubiquitinase USP10 is one of the molecular mechanisms that leads to Tax-mediated induction of ROS [ xref ]."

sparser
"Tax also interacts with the deubiquitinase USP10 to stimulate ROS production, which can contribute to Tax driven-senescence in HTLV-1-infected T cells [ xref ]."

reach
"Tax also interacts with USP10, inhibiting SG assembly and enhancing the production of reactive oxygen species [XREF_BIBR]."

sparser
"Tax mutants and USP10-knockdown indicated that Tax inhibits arsenic-induced SG formation, stimulates ROS production and augments ROS-dependent apoptosis, and they are all dependent on interactions of Tax with USP10."
| PMC

reach
"Tax also interacts with the deubiquitinase USP10 to stimulate ROS production, which can contribute to Tax driven-senescence in HTLV-1-infected T cells [XREF_BIBR]."

sparser
"Tax also interacts with USP10, inhibiting SG assembly and enhancing the production of reactive oxygen species [ xref ]."

reach
"A curved arrowhead with a black color indicates positive regulation.In addition to USP10 being regulated by the proteins of host cells, previous studies of the Takahashi group showed that HTLV-1 Tax interacted with amino acids 727–798 at the C-terminal of USP10 and inhibited USP10’s deubiquitination activity and function."
G3BP2 affects G3BP1
| 10
| 6

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [ xref ], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [ xref ]."

sparser
"However, a role for the G3BP1 and G3BP2 interactions with CAPRIN1 and/or USP10 under chronic nutrient starvation has not yet been investigated."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al.2017); SeV Trailer RNA captures TIAR from SG (Iseni et al.2002); West Nile Virus (WNV) and Dengue virus (DENV) 3′-end viral genome captures TIA-1/TIAR (Li et al.2002; Emara and Brinton 2007; Xia et al.2015); DENV 3′-UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al.2011; Reineke et al.2015); JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al.2011)."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al. 2017) ; SeV Trailer RNA captures TIAR from SG (Iseni et al. 2002) ; West Nile Virus (WNV) and Dengue virus (DENV) 3 0 -end viral genome captures TIA-1/TIAR (Li et al. 2002; Emara and Brinton 2007; Xia et al. 2015) ; DENV 3 0 -UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al. 2011; Reineke et al. 2015) ; JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al. 2011) ."

sparser
"Additional experiments demonstrate that, in contrast to DDX6, the SG proteins G3BP1, G3BP2, Caprin1 and USP10 bind to the variable region (VR) in the 3' UTR."

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [171], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [172]."
| 4

sparser
"Validation of the mass spectrometry data confirmed the interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."

sparser
"NCAP showed specific interaction with the SG proteins G3BP1, G3BP2, YTHDF3, USP10 and PKR, and translocated to SGs following oxidative stress and heat shock."

sparser
"NCAP interacts with SG proteins. (A) Interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."

sparser
"Fig. 4: NCAP interacts with SG proteins. (A) Interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."
CD36 affects USP10
| 4 6
USP10 binds CD36. 6 / 6
| 6

sparser
"Deeply, we sought to the regulation mechanism of CD36-USP10 in macrophages."

sparser
"Besides, we tested the regulation of USP10-CD36 under the oxLDL stimulation."

sparser
"In addition, Co-IP results showed that USP10 interacts with CD36 and stabilizes CD36 protein by cleaving poly-ubiquitin on CD36."

sparser
"Western blot results showed that USP10 interacts with CD36 ( xref , xref )."

sparser
"The regulation of USP10-CD36 may provide a significant therapeutic scheme in atherosclerosis."

sparser
"USP10 interacts with CD36."
| 4

reach
"USP10 interacts with CD36."

reach
"In addition, Co-IP results showed that USP10 interacts with CD36 and stabilizes CD36 protein by cleaving poly-ubiquitin on CD36."

reach
"Co-IP and confocal assays revealed that CD36 interacts USP10 and the co-localization of CD36 and USP10."

reach
"Western blot results showed that USP10 interacts with CD36 (XREF_FIG, XREF_FIG)."
| 4 1

sparser
"However, our results using USP10 functional mutants indicated that ROS inhibition by USP10 is dispensable for USP10 to inhibit apoptosis of HSCs."

reach
"On exposure to arsenic, an oxidative stress inducer, USP10 is recruited into stress granules (SGs), and USP10 containing SGs reduce reactive oxygen species (ROS) production and inhibit ROS dependent apoptosis."

reach
"However, our results using USP10 functional mutants indicated that ROS inhibition by USP10 is dispensable for USP10 to inhibit apoptosis of HSCs."

reach
"Upon exposure to an oxidant, USP10 inhibits ROS dependent apoptosis by reducing ROS production."

reach
"Similarly, USP10 and USP13 silencing could induce ROS generation and cause DNA damage."
| PMC

reach
"USP10-knockout indicated that USP10, through augmenting formation of SGs, reduced reactive oxygen species (ROS) production and inhibited ROS dependent apoptosis."
| PMC

reach
"Since aberrant ROS production has been shown to be a critical apoptosis inducer of HSCs under basal and stress conditions, it was initially assumed that USP10 inhibits apoptosis of HSCs by inhibiting ROS production."

reach
"For example, inhibiting USP10 reduces the production of reactive oxygen species and suppresses apoptosis and the formation of SGs (stress granules) [78]."

reach
"We showed previously that USP10-KD increases the production of ROS by an oxidant (arsenite)."
USP10 affects TG
| 9
| 9

sparser
"Furthermore, hematoxylin and eosin and Picrosirius red staining of heart sections indicated that hearts from USP10TG mice developed less cardiomyocyte hypertrophy and fibrosis in the interstitial and perivascular spaces than their USP10‐NTG littermates (Figure  xref through xref )."

sparser
"Moreover, compared with the USP10‐NTG controls, the USP10TG mice exhibited significantly decreased LVEDd, LVESd, and increased fractional shortening values and ejection fraction values (Figure  xref through xref )."

sparser
"In parallel, the mRNA levels of Anp, Bnp, Myh7, collagen Iα, collagen III, and Ctgf were markedly decreased in the hearts of USP10TG mice compared with those in the hearts of the USP10‐NTG controls (Figure  xref )."

sparser
"We evaluated cardiac parameters in USP10‐NTG and USP10TG mice that were subjected to AB surgery to further confirm the regulatory function of USP10 in CH."

sparser
"Methods and Results Cardiac-specific USP10 knockout (USP10-CKO) mice and USP10-transgenic (USP10-TG) mice were used to examined the role of USP10 in CH following aortic banding."

sparser
"To determine whether USP10 overexpression could reduce pressure overload‐induced CH, we also generated cardiac‐specific USP10 transgenic mice (USP10TG) (Figure  xref and xref )."

sparser
"As expected, the ratio of HW to BW was decreased in USP10TG mice compared with USP10‐NTG group at 4 weeks after AB surgery (Figure  xref )."

sparser
"Cardiac‐specific USP10 knockout (USP10‐CKO) mice and USP10‐transgenic (USP10TG) mice were used to examined the role of USP10 in CH following aortic banding."

sparser
"First, we tested Sirt6 level in USP10‐CKO and USP10TG mice."
USP10 affects SGs
| 9
USP10 inhibits SGs.
| 7
USP10 inhibits SGs. 5 / 5
| 5

reach
"Interestingly, the overexpression of USP10 prevents the formation of SGs by directly binding G3BP via an FGDF motif."

reach
"Cells with an exon 3 deletion may express an FGDF containing inhibitory fragment of USP10, which would suppress SGs as does USP10 1-40 (XREF_FIG) and explain the apparent contradiction."

reach
"Tet induced GFP-USP10 expression prevents AS and CZ induction of SGs (XREF_FIG) but does not alter stress induced polysome disassembly (XREF_FIG), indicating that GFP-USP10 inhibits the cytoplasmic condensation of mRNPs into SGs."

reach
"Here, we have shown that overexpression of EGFP-USP10, but not a mutant lacking an intact FGDF-motif, efficiently blocks the formation of SGs (XREF_FIG)."

reach
"When mCherry tagged USP10 is cotransfected with GFP-G3BP1, both proteins colocalize in SGs (XREF_FIG, 4), but mCherry-USP10 coexpressed with GFP-Caprin1 inhibits SGs even upon AS treatment (XREF_FIG, 5 and 6), suggesting that USP10 and Caprin1 compete for limiting G3BP."
USP10 bound to G3BP1 inhibits SGs. 2 / 2
| 2

reach
"USP10 lacking the PAM2 motif does not co-IP PABP or eIF4G, but still binds G3BP and blocks SGs (XREF_FIG, 10-12)."

reach
"Since the binding of nsP3 and USP10 to G3BP inhibits SGs, we hypothesized that ICP8 binding might do the same."
USP10 activates SGs.
| 2
USP10 activates SGs. 2 / 2
| 2

reach
"Rescue experiments using a G3BP1 mutant that lacks the ability to bind Caprin1 or USP10 rescues SGs assembly, whereas other phosphomimetic mutants of G3BP1 (G3BP1-S149E) fail to do that [XREF_BIBR]."

reach
"In addition, the overexpression of USP10 without stress stimuli in HT22 cells induced TIA1/Tau/USP10 positive SGs in a deubiquitinase independent manner."
USP10 affects IGF2BP3
| 4 5
| 4 5

reach
"Further co-immunoprecipitation assay confirmed the interaction between IGF2BP3 and USP10."

reach
"Moreover, the physiological interaction between the endogenous IGF2BP3 and USP10 was verified in HEK293T cells."

sparser
"Then, to answer which domain of IGF2BP3 is involved in the interaction between IGF2BP3 and USP10, we constructed truncated mutants of IGF2BP3 (Figure xref )."

reach
"Then, to answer which domain of IGF2BP3 is involved in the interaction between IGF2BP3 and USP10, we constructed truncated mutants of IGF2BP3."

sparser
"Further co-immunoprecipitation assay confirmed the interaction between IGF2BP3 and USP10 (Figure xref )."

sparser
"Moreover, the physiological interaction between the endogenous IGF2BP3 and USP10 was verified in HEK293T cells (Figure xref )."

sparser
"Subsequent co-immunoprecipitation assays confirmed the physiological interaction between IGF2BP3 and USP10."

reach
"Subsequent co-immunoprecipitation assays confirmed the physiological interaction between IGF2BP3 and USP10."

sparser
"Notably IGF2BP3 was directly associated with a deubiquitinase Ubiquitin specific peptidase 10 (USP10) and attenuated its function in stabilizing p53 protein."
USP10 affects DnaK
| 2 7
USP10 binds DnaK. 9 / 9
| 2 7

sparser
"DnaK DnaK binds to USP10 and affects p53-dependent anticancer functions."

reach
"We demonstrate that mycoplasma DnaK interacts with and reduces the activities of human proteins involved in critical cellular pathways, including DNA-PK and PARP1, which are required for efficient DNA repair, and binds to USP10 (a key p53 regulator), impairing p53 dependent anticancer functions."

sparser
"At the same time, the interaction of DnaK with USP10 reduces p53 activity, preventing its anticancer effectiveness."

sparser
"Taken together, our results indicate that DnaK binding to USP10 prevents its deubiquitinating activity, thus reducing p53 stability and its anticancer functions and the cellular response to some anticancer drugs."

sparser
"USP10 deubiquitinates p53, regulating the DNA-damage response; DnaK interacts with USP10, inhibiting its deubiquitination activity [34] ."

sparser
"We demonstrated that this mycoplasma’s DnaK binds to human USP10 (ubiquitin carboxyl-terminal hydrolase 10, a regulator of p53 stability), reducing p53 stability and anti-cancer functions, potentially increasing the likelihood of DNA mutations and consequent malignant transformation [ xref ]."

sparser
"Mycoplasma DnaK Binds USP10 and Impairs p53-Dependent Functions."

sparser
"We first confirmed the interaction between DnaK and USP10 by immunoprecipitation studies ( xref )."

reach
"We demonstrated that this mycoplasma’s DnaK binds to human USP10 (ubiquitin carboxyl-terminal hydrolase 10, a regulator of p53 stability), reducing p53 stability and anti-cancer functions, potentially increasing the likelihood of DNA mutations and consequent malignant transformation [107]."
TG affects USP10
| 9
| 9

sparser
"Furthermore, hematoxylin and eosin and Picrosirius red staining of heart sections indicated that hearts from USP10TG mice developed less cardiomyocyte hypertrophy and fibrosis in the interstitial and perivascular spaces than their USP10‐NTG littermates (Figure  xref through xref )."

sparser
"Moreover, compared with the USP10‐NTG controls, the USP10TG mice exhibited significantly decreased LVEDd, LVESd, and increased fractional shortening values and ejection fraction values (Figure  xref through xref )."

sparser
"In parallel, the mRNA levels of Anp, Bnp, Myh7, collagen Iα, collagen III, and Ctgf were markedly decreased in the hearts of USP10TG mice compared with those in the hearts of the USP10‐NTG controls (Figure  xref )."

sparser
"We evaluated cardiac parameters in USP10‐NTG and USP10TG mice that were subjected to AB surgery to further confirm the regulatory function of USP10 in CH."

sparser
"Methods and Results Cardiac-specific USP10 knockout (USP10-CKO) mice and USP10-transgenic (USP10-TG) mice were used to examined the role of USP10 in CH following aortic banding."

sparser
"To determine whether USP10 overexpression could reduce pressure overload‐induced CH, we also generated cardiac‐specific USP10 transgenic mice (USP10TG) (Figure  xref and xref )."

sparser
"As expected, the ratio of HW to BW was decreased in USP10TG mice compared with USP10‐NTG group at 4 weeks after AB surgery (Figure  xref )."

sparser
"Cardiac‐specific USP10 knockout (USP10‐CKO) mice and USP10‐transgenic (USP10TG) mice were used to examined the role of USP10 in CH following aortic banding."

sparser
"First, we tested Sirt6 level in USP10‐CKO and USP10TG mice."
IKBKG affects USP10
4 | 1 4
4 | 1

No evidence text available

No evidence text available

No evidence text available

reach
"[104] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO attached M1 linked polyubiquitin chains, thus inhibiting the genotoxic NF-kappaB signaling cascade."

No evidence text available
| 4

sparser
"NEMO ubiquitylation is decreased through the deubiquitinase USP10, which interacts with NEMO via MCPIP1 upon genotoxic stress."

sparser
"Moreover, usp4-depleted zebrafish (Danio rerio) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment. [102] Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) cleaves K63-linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to highrisk human papillomavirus (hrHPV) infection. [103] TRAF family member-associated NF-B activator (TANK) interacts with both monocyte chemotactic protein-1-induced protein-1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF-B activation in response to TLR activation. [104] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO-attached M1-linked polyubiquitin chains, thus inhibiting the genotoxic NF-B signaling cascade. [40] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."

sparser
"Moreover, usp4 ‐depleted zebrafish ( Danio rerio ) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment. [ xref ] Ubiquitin carboxyl‐terminal hydrolase L1 (UCHL1) cleaves K63‐linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to high‐risk human papillomavirus (hrHPV) infection. [ xref ] TRAF family member‐associated NF‐ κ B activator (TANK) interacts with both monocyte chemotactic protein‐1‐induced protein‐1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF‐ κ B activation in response to TLR activation. [ xref ] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO‐attached M1‐linked polyubiquitin chains, thus inhibiting the genotoxic NF‐ κ B signaling cascade. [ xref ] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."

sparser
"Moreover, usp4‐depleted zebrafish (Danio rerio) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment.[ 102 ] Ubiquitin carboxyl‐terminal hydrolase L1 (UCHL1) cleaves K63‐linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to high‐risk human papillomavirus (hrHPV) infection.[ 103 ] TRAF family member‐associated NF‐κB activator (TANK) interacts with both monocyte chemotactic protein‐1‐induced protein‐1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF‐κB activation in response to TLR activation.[ 104 ] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO‐attached M1‐linked polyubiquitin chains, thus inhibiting the genotoxic NF‐κB signaling cascade.[ 40 ] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."
IGF2BP3 affects USP10
| 4 5
| 4 5

reach
"Further co-immunoprecipitation assay confirmed the interaction between IGF2BP3 and USP10."

reach
"Moreover, the physiological interaction between the endogenous IGF2BP3 and USP10 was verified in HEK293T cells."

sparser
"Then, to answer which domain of IGF2BP3 is involved in the interaction between IGF2BP3 and USP10, we constructed truncated mutants of IGF2BP3 (Figure xref )."

reach
"Then, to answer which domain of IGF2BP3 is involved in the interaction between IGF2BP3 and USP10, we constructed truncated mutants of IGF2BP3."

sparser
"Further co-immunoprecipitation assay confirmed the interaction between IGF2BP3 and USP10 (Figure xref )."

sparser
"Moreover, the physiological interaction between the endogenous IGF2BP3 and USP10 was verified in HEK293T cells (Figure xref )."

sparser
"Subsequent co-immunoprecipitation assays confirmed the physiological interaction between IGF2BP3 and USP10."

reach
"Subsequent co-immunoprecipitation assays confirmed the physiological interaction between IGF2BP3 and USP10."

sparser
"Notably IGF2BP3 was directly associated with a deubiquitinase Ubiquitin specific peptidase 10 (USP10) and attenuated its function in stabilizing p53 protein."
DnaK affects USP10
| 2 7
USP10 binds DnaK. 9 / 9
| 2 7

sparser
"DnaK DnaK binds to USP10 and affects p53-dependent anticancer functions."

reach
"We demonstrate that mycoplasma DnaK interacts with and reduces the activities of human proteins involved in critical cellular pathways, including DNA-PK and PARP1, which are required for efficient DNA repair, and binds to USP10 (a key p53 regulator), impairing p53 dependent anticancer functions."

sparser
"At the same time, the interaction of DnaK with USP10 reduces p53 activity, preventing its anticancer effectiveness."

sparser
"Taken together, our results indicate that DnaK binding to USP10 prevents its deubiquitinating activity, thus reducing p53 stability and its anticancer functions and the cellular response to some anticancer drugs."

sparser
"USP10 deubiquitinates p53, regulating the DNA-damage response; DnaK interacts with USP10, inhibiting its deubiquitination activity [34] ."

sparser
"We demonstrated that this mycoplasma’s DnaK binds to human USP10 (ubiquitin carboxyl-terminal hydrolase 10, a regulator of p53 stability), reducing p53 stability and anti-cancer functions, potentially increasing the likelihood of DNA mutations and consequent malignant transformation [ xref ]."

sparser
"Mycoplasma DnaK Binds USP10 and Impairs p53-Dependent Functions."

sparser
"We first confirmed the interaction between DnaK and USP10 by immunoprecipitation studies ( xref )."

reach
"We demonstrated that this mycoplasma’s DnaK binds to human USP10 (ubiquitin carboxyl-terminal hydrolase 10, a regulator of p53 stability), reducing p53 stability and anti-cancer functions, potentially increasing the likelihood of DNA mutations and consequent malignant transformation [107]."
USP10 affects autophagy
| 1 7
USP10 activates autophagy.
| 1 4
| 1 4

reach
"JNK1/TSC2 signaling pathways were required for USP10-induced autophagy."

reach
"USP10 could promote autophagy-related pathways and molecules and increase the synthesis of autophagosomes in NASH, improving steatosis, inflammation, and fibrosis."

reach
"Spautin-1 inhibits autophagy by reducing the activity of USP10 and USP13, which promotes a major aspect of autophagy: degradation by Vps34 complexes."

eidos
"USP10 has also been shown to enhance autophagy by deubiquitinating beclin-1 , a component of class-III PI3K complexes that catalyze the formation of phosphatidylinositol 3-phosphate at both autophagy initiation and autophagosome maturation steps ( 21 ) ."

reach
"Furthermore, the specific inhibitor to C-jun N-terminal protein kinase-1 (JNK1), DB07268, abolished USP10-induced autophagy."
USP10 inhibits autophagy.
| 3
| 3

reach
"Interestingly, Spautin-1-mediated specific and potent inhibition of deubiquitinating activity of USP10 and USP13 was shown to promote proteasomal degradation of PIK3C3 and thereby inhibit autophagy [179]."

reach
"Because the reductions in the levels of USP10 and USP13 in H4-LC3-GFP cells treated with spautin-1 appeared later than the reductions in the levels of Vps34 complexes and autophagy (XREF_FIG), the reduced levels of USP10 and USP13 are unlikely to be the primary reason for the ability of spautin-1 to reduce the levels of PtdIns3P and inhibit autophagy."

reach
"These results suggested that LC3 is degraded by autophagy in both USP10-WT and USP10-KD cells with or withoutMG-132 treatment, and that USP10-KD does not inhibit autophagy mediated degradation of LC3."
USP10 affects USP10
| 7
USP10 activates USP10.
| 4
USP10 activates USP10. 4 / 5
| 4

reach
"Together, these results suggested that AMPK phosphorylates USP10 at S76, which activates USP10 and facilitates further activation of AMPK under energy stress, forming a feedforward loop.Given the impo[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Zhenkun Lou et al. have found that AMPKα1 or AMPKα2 ubiquitination blocks its phosphorylation by LKB1, which could be rescued by the deubiquitinase ubiquitin specific peptidase 10 (USP10) [54]."

reach
"These results suggest that S76 phosphorylation of USP10 increases the deubiquitinase activity of USP10."

reach
"Together, these results suggested that AMPK phosphorylates USP10 at S76, which activates USP10 and facilitates further activation of AMPK under energy stress, forming a feedforward loop."
USP10 decreases the amount of USP10.
| 3
USP10 decreases the amount of USP10. 3 / 3
| 3

reach
"Concordantly, siRNA knockdown of USP10, which caused a down-regulation of USP10 expression by >80%, did not prevent agonist-induced deubiquitination of the GCGR (Fig. S3, C and D)."

reach
"41 The results showed that USP10 knockdown promoted AID-Dendra2 signal attenuation (Fig. 1h), while the overexpression of USP10 rather than USP10CA, delayed the attenuation of AID-Dendra2 (Fig. 1i)."

reach
"To exclude the probable off-target effects, two more pairs of USP10 shRNAs were used to knockdown the expression of USP10."
USP10 affects NFkappaB
| 1 3
USP10 inhibits NFkappaB.
| 1 2
| 1 2

eidos
"A previous study demonstrated that USP10 inhibits NF-kappaB activation and inflammatory cytokine induction ( 22 ) ."

reach
"For example, USP10 can inhibit genotoxic NF-kappaB activation through monocyte chemotactic protein-1-induced protein-1 (MCPIP1)-facilitated deubiquitination of NEMO [XREF_BIBR]."

reach
"Suppression of genotoxic NF-kappaB activation by MCPIP1 and USP10."
USP10 activates NFkappaB.
| 1
| 1

reach
"In accordance, depletion of USP10 enhanced NF-kappaB activation induced by IL-1beta or LPS."
USP10 affects MDM2
1 | 5 1
USP10 inhibits MDM2.
| 1
USP10 inhibits MDM2. 1 / 2
| 1

reach
"25 As a tumour suppressor gene, USP10 was found to reverse the effect of Mdm2 on the exocytosis and degradation of p53."
USP10 deubiquitinates MDM2.
| 2
USP10 deubiquitinates MDM2. 2 / 2
| 2

reach
"Another DUB, USP10 deubiquitinates p53, but not MDM2 or MDMX."

reach
"USP10 (a cytosolic deubiquitinating enzyme) with an affinity for p53 reverses Mdm2-mediated ubiquitination, cytoplasmatic degradation, and nuclear export and impacts the transcriptional activity of p53 (Sun and Dai, 2014)."
USP10 binds MDM2.
1 | 1
1 | 1

No evidence text available

sparser
"Notably, USP10 does not bind to MDM2 [ xref ]."
USP10 activates MDM2.
| 2
USP10 activates MDM2. 2 / 2
| 2

reach
"However, coexpression of USP10 or HAUSP, but not USP10 CA or USP10Delta1-100, significantly reversed the inhibitory effect of Mdm2 on p53 mediated apoptosis."

reach
"In contrast to USP7 and USP2a, USP10 specifically deubiquitinate p53 because knockdown of USP10 in HCT116 p53-/- cells does not cause Mdm2 reduction [XREF_BIBR]."
USP10 affects EIF4G1
4 | 4
4 | 4

No evidence text available

sparser
"However, the underlying mechanisms for USP10 interacting with EIF4G1 and regulatory functions in NSCLC still require further investigation."

sparser
"By using co-immunoprecipitation and immunofluorescence approaches, we further confirmed the interaction of EIF4G1 and USP10 in H1299 cells (Figure xref )."

No evidence text available

sparser
"There is a recent report that USP10 interacts with eIF4G1 and depletion of USP10 enhanced eIF4G1 protein levels( xref )."

No evidence text available

sparser
"It was reported that EIF4G1 interacted with the ubiquitin USP10, as shown by tandem affinity purification and mass spectrometry; USP10 is located in the cytoplasm, and USP10 had been shown to be able to specifically ubiquitin and stabilize p53, thereby regulating p53‐dependent downstream function. xref , xref , xref To date, p53 is one of the most important tumour suppressor factors. xref As a transcription factor, p53 regulates a variety of cytological responses by regulating downstream target genes. xref , xref For example, p53 regulates cell cycle G1 arrest by acting on the CDK inhibitory factor p21, cell apoptosis by acting on bax, energy metabolism by acting on TIGAR of glycolysis, and autophagy by acting on AMPK."

No evidence text available
MAPT affects USP10
| 3 5
| 3 5

sparser
"Inhibition of USP10-Tau interaction might be therapeutically useful in the management of AD and related tauopathies."

reach
"Results from mass spectrometry, reciprocal immunoprecipitation, and immunofluorescence assays strongly prove Tau's interaction with USP10."

reach
"Moreover, in primary neuronal cultures, Aβ 42 induces a dose-dependent USP10 upregulation, an increase in the levels of both total and phosphorylated Tau, as well as a markedly elevated Tau binding with USP10, that is accompanied by a significantly decreased Tau ubiquitination."

sparser
"Results from mass spectrometry, reciprocal immunoprecipitation, and immunofluorescence assays strongly prove Tau's interaction with USP10."

sparser
"This is further supported by the Tau307-326K and Tau341-378K peptides' competitive inhibition of Tau binding with USP10, attenuating Tau hyperphosphorylation and Tau deubiquitination."

reach
"This is further supported by the Tau307-326K and Tau341-378K peptides' competitive inhibition of Tau binding with USP10, attenuating Tau hyperphosphorylation and Tau deubiquitination."

sparser
"Moreover, in primary neuronal cultures, Aβ 42 induces a dose-dependent USP10 upregulation, an increase in the levels of both total and phosphorylated Tau, as well as a markedly elevated Tau binding with USP10, that is accompanied by a significantly decreased Tau ubiquitination."

sparser
"The association of ubiquitin with neurofibrillary tangles is also well documented, xref in line with shreds of evidence related to Ubiquitin‐specific protease 10 (USP10) as a crucial factor for the formation of SGs comprising tau, TIA‐1, and USP10. xref USP10 also colocalizes with aggregated tau in AD patients' brain lesions, xref suggesting its role in SG mediated tau aggregation."
FLT3 affects USP10
| 2 6
| 2 6

reach
"The data in our report demonstrated that ATO treatment reduces the binding of USP10 to FLT3 protein, which might partly contribute to the enhanced ubiquitination of mutant FLT3 protein.Hsp90 is an important chaperone molecule assisting in folding and preventing client proteins from ubiquitination and degradation [44]."

sparser
"A similar interaction between USP10 and FLT3 was demonstrated in 293T cells engineered to exogenously express these proteins ( xref )."

reach
"Co-immunoprecipitation assays showed that ATO treatment led to significantly reduced binding of USP10 with FLT3 protein in TF1/ITD-Ub cells."

sparser
"Co-immunoprecipitation assays showed that ATO treatment led to significantly reduced binding of USP10 with FLT3 protein in TF1/ITD-Ub cells."

sparser
"The data in our report demonstrated that ATO treatment reduces the binding of USP10 to FLT3 protein, which might partly contribute to the enhanced ubiquitination of mutant FLT3 protein."

sparser
"Our current data also support our previous observation that FLT3 and USP10 bind to one another, xref as FLT3 and USP10 co-immunoprecipitate in Ba/F3-FLT3-ITD cells (an AKT immunoprecipitation was included as a control for nonspecific binding) (Fig.  xref , lower panel)."

sparser
"ATO facilitates poly-ubiquitination and degradation of FLT3 partly through reducing the binding of mutant FLT3 to the deubiquitinating enzyme USP10."

sparser
"We observed robust co-immunoprecipitation (co-I.P.) of USP10 with FLT3 in Ba/F3-FLT3-ITD cells; reverse co-I.P. studies confirmed the association of FLT3 with USP10 ( xref )."
EIF4G1 affects USP10
4 | 4
4 | 4

No evidence text available

sparser
"However, the underlying mechanisms for USP10 interacting with EIF4G1 and regulatory functions in NSCLC still require further investigation."

sparser
"By using co-immunoprecipitation and immunofluorescence approaches, we further confirmed the interaction of EIF4G1 and USP10 in H1299 cells (Figure xref )."

No evidence text available

sparser
"There is a recent report that USP10 interacts with eIF4G1 and depletion of USP10 enhanced eIF4G1 protein levels( xref )."

No evidence text available

sparser
"It was reported that EIF4G1 interacted with the ubiquitin USP10, as shown by tandem affinity purification and mass spectrometry; USP10 is located in the cytoplasm, and USP10 had been shown to be able to specifically ubiquitin and stabilize p53, thereby regulating p53‐dependent downstream function. xref , xref , xref To date, p53 is one of the most important tumour suppressor factors. xref As a transcription factor, p53 regulates a variety of cytological responses by regulating downstream target genes. xref , xref For example, p53 regulates cell cycle G1 arrest by acting on the CDK inhibitory factor p21, cell apoptosis by acting on bax, energy metabolism by acting on TIGAR of glycolysis, and autophagy by acting on AMPK."

No evidence text available
AICDA affects USP10
| 3 5
| 3 5

sparser
"Furthermore, the specific interaction between internal USP10 and AID was confirmed in CH12 cells (Fig. xref )."

reach
"The interaction of internal USP10 and AID was determined with IP and western blot assay."

sparser
"Furthermore, the specific interaction between internal USP10 and AID was confirmed in CH12 cells (Fig. 1d) ."

sparser
"The interaction of internal USP10 and AID was determined with IP and western blot assay."

reach
"Besides, the GST pull-down assay confirmed that AID directly bound with USP10 (Supplementary Fig. 1f)."

sparser
"Besides, the GST pull-down assay confirmed that AID directly bound with USP10 ( Supplementary Fig. 1f )."

reach
"Furthermore, the specific interaction between internal USP10 and AID was confirmed in CH12 cells (Fig. 1d)."

sparser
"Besides, the GST pull-down assay confirmed that AID directly bound with USP10 (Supplementary Fig. xref )."
USP10 affects TOP2alpha
| 7
USP10 binds TOP2alpha.
| 4
USP10 binds TOP2alpha. 4 / 4
| 4

reach
"In this study, we also report that in addition to RNF168, TOP2alpha also interacts with USP10."

reach
"Given that our mass spectrometry analysis identified USP10 as a possible RNF168 interacting partner (XREF_SUPPLEMENTARY), we hypothesized that USP10 and RNF168 forms a complex with TOP2alpha to co-regulate its ubiquitylation level."

reach
"These novel functional interactions between RNF168, TOP2alpha and USP10 are likely to contribute to the maintenance of chromosomal integrity."

reach
"Finally, we show that the deubiquitylase USP10 interacts with RNF168 and TOP2alpha, and restrains ubiquitylation of TOP2alpha as well as its chromatin binding."
USP10 deubiquitinates TOP2alpha.
| 2
USP10 deubiquitinates TOP2alpha. 2 / 2
| 2

reach
"USP10 deubiquitylates TOP2alpha and restrains its chromatin binding."

reach
"We also identified USP10 as an interacting partner for RNF168 and TOP2alpha, and provide evidence that USP10 deubiquitylates TOP2alpha and negatively regulates its chromatin binding (XREF_FIG)."
USP10 inhibits TOP2alpha.
| 1
USP10 inhibits TOP2alpha. 1 / 1
| 1

reach
"However, additional overexpression of USP10-WT in these cells significantly reduced the fraction of chromatin bound TOP2alpha (XREF_FIG, cf. lane 3 versus lane 4)."
USP10 affects MSH2
2 1 | 3 1
USP10 binds MSH2.
2 | 1
2 | 1

No evidence text available

sparser
"However, the clinicopathological features and survival analysis indicated that the USP10 and MSH2 proteins were not associated with clinical features, including age, sex, tumor size, Tumor-Node-Metastasis stage and tumor cell differentiation, along with the prognosis of NSCLC."

No evidence text available
USP10 deubiquitinates MSH2.
1 | 1
USP10 deubiquitinates MSH2. 2 / 2
1 | 1

reach
"USP10 deubiquitinates MSH2 in vitro and in vivo Moreover, the protein level of MSH2 is positively correlated with the USP10 protein level in a panel of lung cancer cell lines."

"Here we report that ubiquitin-specific peptidase 10 (USP10) interacts with and stabilizes MSH2."
USP10 activates MSH2.
| 2
USP10 activates MSH2. 2 / 2
| 2

reach
"So, HDAC6 and USP10 exert opposing effect on MSH2 : HDAC6 promotes degradation of MSH2, while USP10 promotes stabilization of MSH2."

reach
"However, it seems to contradict the finding that USP10 promotes sensitivity to 6-TG by stabilizing MSH2 41."
USP10 affects HDAC7
| 1 2 4
USP10 binds HDAC7.
| 1 4
| 1 4

sparser
"Taken together, our results demonstrate that USP10 interacts with HDAC7 and stabilize HDAC7 protein through promoting HDAC7 deubiquitination."

sparser
"Next, to gain more insight into the USP10-HDAC7 interaction, a series of GST-tagged truncated mutants of USP10 and HA-tagged HDAC7 were co-transfected, and the C-terminal region (701–798 aa) of USP10 was determined as the binding region with HDAC7 (Fig.  xref f, g)."

sparser
"Therefore, promotion of USP10-HDAC7 axis drives ESCC cell growth."

sparser
"After screening the potential deubiquitinating enzymes of HDAC7, we first reported deubiquitinase USP10 could bind and stabilize HDAC7 protein in ESCC cells."

reach
"Furthermore, deubiquitinase USP10 interacted with and stabilized HDAC7."
USP10 activates HDAC7.
| 1 1
USP10 activates HDAC7. 2 / 2
| 1 1

eidos
"The suppression of USP10 significantly accelerated the degradation of HDAC7 and weakened NSCLC growth and migration ."

reach
"The suppression of USP10 significantly accelerated the degradation of HDAC7 and weakened NSCLC growth and migration."
SYK affects USP10
| 3 4
| 3 4

reach
"To determine whether the USP10 could interact with SYK directly, we performed an in vitro binding assay with purified recombinant His USP10 and GST-SYK proteins."

sparser
"Purified His-USP10 was able to interact with GST-SYK under cell-free conditions, suggesting a direct interaction between USP10 and SYK (Fig.  xref and Supplementary Fig.  xref )."

reach
"We demonstrate a potential physical association between SYK and USP10, as both proteins co-immunoprecipitate in Ba/F3-SYK-TEL cells (a Fes immunoprecipitation was included as a control for nonspecific binding)."

sparser
"USP10 can directly interact with SYK [62] ."

reach
"Purified His USP10 was able to interact with GST-SYK under cell-free conditions, suggesting a direct interaction between USP10 and SYK."

sparser
"To investigate whether SYK and USP10 physically associate in a protein complex, we performed co-immunoprecipitation experiments."

sparser
"To determine whether the USP10 could interact with SYK directly, we performed an in vitro binding assay with purified recombinant His-USP10 and GST-SYK proteins."
P22077 affects USP10
| 2 5
P22077 inhibits USP10. 7 / 7
| 2 5

reach
"Notably, P22077, HBX19818, and HBX19818 analogs including Compounds 3, 7, and 9 also inhibit USP10 in mutant-FLT3-expressing cells."

reach
"Moreover, P22077 still strongly inhibits the viability of USP7 or USP10 stably knockdown K562 cells."

reach
"It has been reported that P22077 also inhibits the activity of USP7 and USP10."

eidos
"P22077 inhibited only USP7 , USP9x , USP10 and USP20 appreciably at 1 muM ( USP47 was not tested ) ."

eidos
"Along with USP7 , P22077 and HBX19818 has also been reported to inhibit USP10 , promoting degradation of FLT3-mutant AML cells [ 174 ] ."

reach
"These results indicate that the combination of cisplatin and USP10 inhibition by P22077 exhibits a more potent inhibition of tumor growth than either single agent treatment, and suggest a novel strategy of combining USP10 inhibition with cisplatin for treatment of NSCLC."

reach
"However, a recent study (Weisberg et al., 2017) revealed that P22077 and HBX19818 may also inhibit the deubiquitinase activity of USP10 and induce the proliferation inhibition of mutant-FLT3 (FLT3-ITD)-positive cancer cells."
Cif affects USP10
| 7
Cif inhibits USP10. 7 / 7
| 7

reach
"Cif inhibited ubiquitin specific protease 10 (USP10), a deubiquitinating enzyme, resulting in increased poly-ubiquitination and proteasomal degradation of TAP1."

reach
"Using this assay, we demonstrated that USP10 activity was inhibited 49 +/-4% by Cif (XREF_FIG)."

reach
"These data are consistent with the view that Cif reduces USP10 activity and thereby increases the amount of multi ubiquitinated CFTR and its degradation in the lysosome."

reach
"For example, in this and a previous study we observed that neither USP34, nor USP8 deubiquitinate CFTR, and only USP10 activity was inhibited by Cif XREF_BIBR, XREF_BIBR."

reach
"Cif inactivates USP10 in the early endosome."

reach
"If G3BP1 knockdown prevented the Cif mediated inhibition of USP10 activity, we would predict that knockdown of G3BP1 would also eliminate the increase in multi ubiquitinated CFTR and degradation of CFTR induced by Cif."

reach
"The next set of experiments was designed to elucidate how Cif inhibits USP10 activity."
CAPRIN1 affects G3BP1, and USP10
| 7
| 7

sparser
"However, a recent study suggests that G3BP proteins interact with Caprin1 and USP10 but that they are mutually exclusive allowing the binding of these proteins to serve as a switch for SG formation depending on the binding partner interacting with G3BP1."

sparser
"However, it is important to note that the interactions between G3BP1 and USP10 or Caprin1 do not fully dictate SG disassembly or assembly, as two different laboratories recently demonstrated that the unstressed G3BP1 interactome significantly overlaps the interactome of G3BP1 during stress [ xref , xref ]."

sparser
"USP10 and Caprin1 antagonistically bind G3BP to regulate SG formation."

sparser
"Our results indicated that N expression abrogates G3BP1 interaction with the SG proteins CAPRIN1 and USP10 ( Figure 2C ), reinforcing the idea that N inhibits the formation of SGs in host cells by sequestering G3BPs away from their interacting SG-nucleating proteins."

sparser
"Rescue experiments using G3BP1 mutants show that phosphomimetic G3BP1-S149E fails to rescue SG formation, whereas G3BP1-F33W, a mutant unable to bind G3BP partner proteins Caprin1 or USP10, rescues SG formation."

sparser
"Therefore, although Caprin1 and USP10 both bind G3BP ( xref ; xref ), they have antagonistic effects on SGs."

sparser
"Interaction of Dengue RNA with stress granule proteins G3BP1, USP10 and caprin1 occurs at an adjacent unstructured region of the 3′ UTR ( xref )."
FldA affects USP10
| 6
| 6

sparser
"Moreover, hematoxylin and eosin and Picrosirius red staining of heart sections indicated that compared with those of their USP10Flox littermates, hearts from USP10‐CKO mice developed significant cardiomyocyte hypertrophy and fibrosis in the interstitial and perivascular spaces (Figure  xref through xref )."

sparser
"In parallel, the mRNA levels of CH‐ and fibrosis‐related genes, including Anp, Bnp, Myh7, collagen Iα, collagen III, and Ctgf, were markedly increased in the hearts of USP10‐CKO mice compared with those in the hearts of USP10Flox controls (Figure  xref )."

sparser
"Furthermore, the USP10‐CKO mice exhibited significantly increased LVEDd and LVESd values and decreased fractional shortening% and ejection fraction values compared with USP10Flox controls (Figure  xref through xref )."

sparser
"Subsequently, USP10Flox and USP10‐CKO mice were subjected to AB surgery, and cardiac parameters were evaluated."

sparser
"As shown in Figure  xref , the ratios of heart weight (HW) to body weight (BW) increased in USP10‐CKO mice compared with USP10Flox group at 4 weeks after AB surgery (Figure  xref )."

sparser
"Western blotting results demonstrated that phosphorylation levels of Akt, GSK3β, mTOR, and p70‐S6K were significantly increased in hypertrophic hearts in USP10‐CKO mice compared with those in USP10Flox controls in response to AB surgery (Figure  xref )."
USP10 affects hepatic steatosis
| 6
USP10 inhibits hepatic steatosis.
| 4
USP10 inhibits hepatic steatosis. 4 / 4
| 4

eidos
"31 Recently , a report suggested that USP10 can inhibit hepatic steatosis , insulin resistance , and inflammation through Sirt6 in nonalcoholic fatty liver disease ."

eidos
"In a non-alcoholic fatty liver disease model , USP10 inhibits hepatic steatosis , insulin resistance , and the inflammatory response by interacting with SIRT6 and inhibiting its ubiquitination and degradation ( 6 ) , and USP10 inhibition also abolishes Mirt2 ( long non-coding RNA myocardial infarction associated transcript 2 ) overexpression-induced suppression of glucose production and lipogenesis in hepatocytes ( 7 ) ."

eidos
"It has been demonstrated that USP10 inhibits hepatic steatosis and insulin resistance via Sirt6 , in which Sirt6 represses the transcription levels of SREBP1 / SREBP2 and their target genes [ 114 ] ."

eidos
"For example , USP10 expression is decreased and upregulated in hepatic steatosis models to inhibit hepatic steatosis , insulin resistance , and inflammation [ 7 ] ."
USP10 activates hepatic steatosis.
| 2
USP10 activates hepatic steatosis. 2 / 2
| 2

eidos
"Ubiquitin-specific peptidase 10 ameliorates hepatic steatosis in nonalcoholic steatohepatitis model by restoring autophagic activity ."

eidos
"USP10 alleviated hepatic steatosis in NASH in an autophagy-dependent manner ."
USP10 affects fldA
| 6
| 6

sparser
"Moreover, hematoxylin and eosin and Picrosirius red staining of heart sections indicated that compared with those of their USP10Flox littermates, hearts from USP10‐CKO mice developed significant cardiomyocyte hypertrophy and fibrosis in the interstitial and perivascular spaces (Figure  xref through xref )."

sparser
"In parallel, the mRNA levels of CH‐ and fibrosis‐related genes, including Anp, Bnp, Myh7, collagen Iα, collagen III, and Ctgf, were markedly increased in the hearts of USP10‐CKO mice compared with those in the hearts of USP10Flox controls (Figure  xref )."

sparser
"Furthermore, the USP10‐CKO mice exhibited significantly increased LVEDd and LVESd values and decreased fractional shortening% and ejection fraction values compared with USP10Flox controls (Figure  xref through xref )."

sparser
"Subsequently, USP10Flox and USP10‐CKO mice were subjected to AB surgery, and cardiac parameters were evaluated."

sparser
"As shown in Figure  xref , the ratios of heart weight (HW) to body weight (BW) increased in USP10‐CKO mice compared with USP10Flox group at 4 weeks after AB surgery (Figure  xref )."

sparser
"Western blotting results demonstrated that phosphorylation levels of Akt, GSK3β, mTOR, and p70‐S6K were significantly increased in hypertrophic hearts in USP10‐CKO mice compared with those in USP10Flox controls in response to AB surgery (Figure  xref )."
USP10 affects YAP1
| 1 5
USP10 deubiquitinates YAP1.
| 4
USP10 deubiquitinates YAP1. 4 / 4
| 4

reach
"In the context of HCC, USP10 promotes HCC cell proliferation and metastasis by deubiquitinating and stabilizing YAP and TAZ, the effector transcription factors of the Hippo signaling pathway, and SMAD4, the major signaling effector of TGFβ signaling [39,40,]."

reach
"USP10 depletion enhanced the polyubiquitination of YAP/TAZ, promoted their proteasomal degradation, and ultimately arrested the proliferation of hepatocellular carcinoma in vitro and in vivo [38]."

reach
"USP10 knockdown increased ubiquitination and degradation of YAP1, which further decreased the PD-L1 and Galectin-9 expression, suppressed immune escape, and reduced the proliferation and metastasis of PAAD cells in vitro and in vivo."

reach
"In conclusion, this work demonstrates that USP10 inhibits YAP1 ubiquitination and degradation to promote Cyr61 expression, which induces immune escape and promotes growth and metastasis of PAAD."
USP10 activates YAP1.
| 1 1
USP10 activates YAP1. 2 / 2
| 1 1

eidos
"In addition , we showed that USP10 knockdown decreased the levels of YAP1 , which is an important positive regulator of migration and invasion in many cancers ."

reach
"Deubiquitinating enzyme USP10 promotes osteosarcoma metastasis and epithelial-mesenchymal transition by stabilizing YAP1."
USP10 affects TOP2α
| 6
USP10 binds TOP2α. 4 / 4
| 4

sparser
"In this study, we also report that in addition to RNF168, TOP2α also interacts with USP10."

sparser
"However, similar to RNF168, USP10 interaction with TOP2α was reduced in response to radiation ( xref )."

sparser
"Examination of synchronized MEFs, in response to serum starvation, aphidicolin or colcemid treatments, indicated no changes of USP10-TOP2α interaction ( xref )."

sparser
"Furthermore, we also observed interaction of USP10 with TOP2α ( xref )."
USP10 binds RNF168 and TOP2α. 2 / 2
| 2

sparser
"These novel functional interactions between RNF168, TOP2α and USP10 are likely to contribute to the maintenance of chromosomal integrity."

sparser
"Given that our mass spectrometry analysis identified USP10 as a possible RNF168-interacting partner ( xref ), we hypothesized that USP10 and RNF168 forms a complex with TOP2α to co-regulate its ubiquitylation level."
USP10 affects TBP
1 1 | 3
USP10 deubiquitinates TBP.
1 | 3
USP10 deubiquitinates TBP. 3 / 3
1 | 2

reach
"To test whether USP10 deubiquitinates and stabilizes TBP in mammalian cells, we overexpressed USP10 in 293T cells and monitored TBP ubiquitination levels in these cells."

reach
"To test whether USP10 deubiquitinates TBP through direct protein protein interactions, we used HA-TBP to co-IP with Flag-USP10 co-expressed in 293T cells."

"Huwe1 as an E3 ligase targeting TBP for K48-linked ubiquitination and proteasome-mediated degradation; We found that Huwe1 activity on TBP is antagonized by the deubiquitinase USP10, which protects TBP from degradation."
Modified USP10 leads to the deubiquitination of TBP. 1 / 1
| 1

reach
"Strikingly, overexpression of USP10 significantly inhibited TBP ubiquitination (XREF_FIG)."
USP10 binds TBP.
1 |
1 |

No evidence text available
USP10 affects Proteasome
| 5
| 5

reach
"Based on USP10 induced ubiquitinated protein aggregation, we hypothesized that coexpression of USP10 with an aggregation-prone protein inhibits proteasome activity."

reach
"Thus, USP10 and p62 might inhibit proteasome activity by trapping proteasome components in protein aggregates and/or aggresomes."

reach
"Moreover, a proteasome activity reporter YFP-CL1 indicated that USP10 together with YFP-CL1 inhibits proteasome activity (XREF_FIG F)."

reach
"p62 and USP10 have been shown to inhibit the proteasome activity by promoting ubiquitinated protein aggregation ."

reach
"These results suggested that coexpression of USP10 with an aggregation-prone protein inhibits proteasome activity to increase the amount of insoluble ubiquitinated proteins, thereby augmenting aggresome formation."
USP10 affects MTOR
| 1 5
USP10 inhibits MTOR.
| 1 3
USP10 inhibits MTOR. 4 / 4
| 1 3

reach
"These results suggesting that loss of USP10 can lead to activation of mTOR signaling in vivo."

eidos
"Moreover , numerous studies have demonstrated that USP10 inhibited mTOR activation and promoted oncogene-induced senescence to exert a tumor-suppressive effect ( 50 , 51 ) ."

reach
"Specifically, USP10 activates PTEN by preventing its K63 linked polyubiquitination mediated by TRIM25 and suppresses the AKT and mTOR signaling pathway thereby inhibiting NSCLC proliferation, indicating that it may be a potential drug target for cancer treatment."

reach
"These results indicate that USP10 may inhibit mTOR activation through regulating PTEN/AKT/PI3K and AMPK signaling pathways in HCC cells.To further dissect how USP10 inhibits mTOR activity, we investig[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP10 activates MTOR.
| 2
USP10 activates MTOR. 2 / 2
| 2

reach
"USP10 suppresses tumor progression by inhibiting mTOR activation in hepatocellular carcinoma."

reach
"Taken together, these results revealed that mTOR signaling pathway plays a critical role in USP10 downregulated HCC cells.Next, we investigated whether USP10 mediated stabilization of PTEN and AMPKalp[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP10 affects Histone
| 6
USP10 deubiquitinates Histone.
| 3
USP10 deubiquitinates Histone. 3 / 3
| 3

reach
"USP10 deubiquitylates the histone variant H2A.Z and both are required for androgen receptor mediated gene activation."

reach
"Moreover, USP10 not only binds AR, resulting in increased transcriptional activity, but also deubiquitinates the histone variant H2A.Z, both of which are required for AR - mediated gene activation [XREF_BIBR, XREF_BIBR]."

reach
"In addition, more bulky structures, SUMO (small ubiquitin related modifier) and ubiquitin are conjugated to histones (Galanty et al., 2009; Morris et al., 2009; Shioo and Eisenman, 2003; Thakar et al.[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP10 activates Histone.
| 3
USP10 activates Histone. 3 / 3
| 3

reach
"To test whether USP10 targets histones as substrates, we co-expressed Flag tagged USP10 (Flag-USP10) with tagged H2A.Z or H2A in 293T cells and measured the levels of ubiquitylated histones by Western blot."

reach
"Importantly, increased USP10 expression did not affect the levels of endogenous H2Bub1 (XREF_FIG B), indicating that USP10 specifically targets the H2A family of histones."

reach
"Supporting our finding that USP10 targets histones, cells with stable knockdown of USP10 consistently have higher levels of H2A.Zub1 and H2Aub1, whereas the levels of H2Bub1 remain unchanged (XREF_FIG D)."
TOP2α affects USP10
| 6
USP10 binds TOP2α. 4 / 4
| 4

sparser
"In this study, we also report that in addition to RNF168, TOP2α also interacts with USP10."

sparser
"However, similar to RNF168, USP10 interaction with TOP2α was reduced in response to radiation ( xref )."

sparser
"Examination of synchronized MEFs, in response to serum starvation, aphidicolin or colcemid treatments, indicated no changes of USP10-TOP2α interaction ( xref )."

sparser
"Furthermore, we also observed interaction of USP10 with TOP2α ( xref )."
USP10 binds RNF168 and TOP2α. 2 / 2
| 2

sparser
"These novel functional interactions between RNF168, TOP2α and USP10 are likely to contribute to the maintenance of chromosomal integrity."

sparser
"Given that our mass spectrometry analysis identified USP10 as a possible RNF168-interacting partner ( xref ), we hypothesized that USP10 and RNF168 forms a complex with TOP2α to co-regulate its ubiquitylation level."
SKP2 affects USP10
| 4 2
| 4 2

reach
"By further mapping the regions of USP10, we showed that the N-terminal region (1-205aa) was required for the binding of USP10 to SKP2."

reach
"Mechanically, USP10 can bind to Skp2 and stabilize its protein level by removing polyubiquitin on Skp2 in the cytoplasm."

reach
"We also validated the interaction between exogenous USP10 and SKP2 using Co-IP in HEK293 cells that were co-transfected with FLAG-USP10, FLAG-USP13, and HA-SKP2 plasmids."

sparser
"Mechanically, USP10 can bind to Skp2 and stabilize its protein level by removing polyubiquitin on Skp2 in the cytoplasm."

sparser
"By further mapping the regions of USP10, we showed that the N-terminal region (1-205aa) was required for the binding of USP10 to SKP2 (Fig.  xref )."

reach
"We found that SKP2 interacted with USP10, USP13, UCHL5, USP14, and USP7 in KBM5-T315I cells."
PIK3C3 affects USP10
| 6
PIK3C3 inhibits USP10.
| 2
PIK3C3 inhibits USP10. 2 / 2
| 2

reach
"Using an imaging-based screen, Liu et al. (2011) recently identified a highly potent small molecule inhibitor of autophagy they named spautin-1 (specific and potent autophagy inhibitor 1), which promotes degradation of the Vps34 complexes via inhibiting ubiquitin-specific processing protease 10 (USP10) and USP13, two ubiquitin-specific peptidases that target the deubiquitination of Beclin1.2."

reach
"It is interesting to note that the knock-down of VPS34 and BECLIN 1 also causes the degradation of USP10 and USP13, which indicates the existence of a regulatory feedback loop [XREF_BIBR]."
PIK3C3 activates USP10.
| 2
PIK3C3 activates USP10. 2 / 2
| 2

reach
"Another interesting observation made in the Spautin-1 study is that knock-down of VPS34 and Beclin 1 causes instability of USP10 and USP13, which indicates the existence of a regulatory feedback loop [XREF_BIBR]."

reach
"The close functional interconnection between the central PI3K-III subunits and the DUBs is demonstrated by the interesting observation that knock-down of VPS34 and Beclin 1 causes instability of USP10 and USP13, which strongly indicates the existence of a regulatory feedback loop [XREF_BIBR]."
PIK3C3 increases the amount of USP10.
| 1
PIK3C3 increases the amount of USP10. 1 / 1
| 1

reach
"Unexpectedly, we found that the knockdown of Beclin1 or Vps34 could also reduce the endogenous levels of USP10 and USP13 (XREF_FIG)."
PIK3C3 binds USP10.
| 1
| 1

reach
"Consistent with this possibility, the effect of spautin-1 on the levels of USP10 and Vps34 complexes can be fully restored in the presence of MG132 (XREF_SUPPLEMENTARY)."
NLRP7 affects USP10
| 3 3
| 3 3

reach
"Furthermore, we found that NLRP7 in CRC cells interacted with ubiquitin specific protease 10 (USP10), which mediated its deubiquitination, thereby increasing its protein stability."

sparser
"Binding of endogenous USP10 to NLRP7 was validated by IP assay with antibody against USP10 (Fig. xref b, lower)."

reach
"Binding of endogenous USP10 to NLRP7 was validated by IP assay with antibody against USP10."

reach
"USP10 interacts with NLRP7 and is highly expressed in CRC."

sparser
"NLRP7 interacts with USP10 to catalyze deubiquitination in colorectal cancer (CRC) cells."

sparser
"USP10 interacts with NLRP7 and is highly expressed in CRC."
G3BP2 affects CAPRIN1
| 6
| 6

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [ xref ], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [ xref ]."

sparser
"However, a role for the G3BP1 and G3BP2 interactions with CAPRIN1 and/or USP10 under chronic nutrient starvation has not yet been investigated."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al.2017); SeV Trailer RNA captures TIAR from SG (Iseni et al.2002); West Nile Virus (WNV) and Dengue virus (DENV) 3′-end viral genome captures TIA-1/TIAR (Li et al.2002; Emara and Brinton 2007; Xia et al.2015); DENV 3′-UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al.2011; Reineke et al.2015); JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al.2011)."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al. 2017) ; SeV Trailer RNA captures TIAR from SG (Iseni et al. 2002) ; West Nile Virus (WNV) and Dengue virus (DENV) 3 0 -end viral genome captures TIA-1/TIAR (Li et al. 2002; Emara and Brinton 2007; Xia et al. 2015) ; DENV 3 0 -UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al. 2011; Reineke et al. 2015) ; JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al. 2011) ."

sparser
"Additional experiments demonstrate that, in contrast to DDX6, the SG proteins G3BP1, G3BP2, Caprin1 and USP10 bind to the variable region (VR) in the 3' UTR."

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [171], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [172]."
FOXO4 affects USP10
| 1 5
FOXO4 increases the amount of USP10.
| 3
FOXO4 increases the amount of USP10. 3 / 3
| 3

reach
"In addition, FoxO4 modulated USP10 transcription."

reach
"This study aimed to investigate whether forkhead box O4 (FoxO4) could negatively modulate ubiquitin specific peptidase 10 (USP10) transcription to aggravate the apoptosis and oxidative stress of hypoxia and reoxygenation (H/R)-induced cardiomyocytes through Hippo and YAP pathway."

reach
"In conclusion, FoxO4 negatively modulated USP10 transcription to aggravate the apoptosis and oxidative stress of H/R induced H9C2 cells via blocking Hippo and YAP pathway."
FOXO4 decreases the amount of USP10.
| 1 2
FOXO4 decreases the amount of USP10. 3 / 3
| 1 2

reach
"FoxO4 overexpression could reverse the protective effects of USP10 overexpression on H/R induced H9C2 cells by regulating the Hippo and YAP signaling pathway."

reach
"FoxO4 negatively modulates USP10 transcription to aggravate the apoptosis and oxidative stress of hypoxia and reoxygenation induced cardiomyocytes by regulating the Hippo and YAP pathway."

trips
"FoxO4 negatively modulates USP10 transcription to aggravate the apoptosis and oxidative stress of hypoxia/reoxygenation-induced cardiomyocytes by regulating the Hippo/YAP pathway."
CAPRIN1 affects G3BP2
| 6
| 6

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [ xref ], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [ xref ]."

sparser
"However, a role for the G3BP1 and G3BP2 interactions with CAPRIN1 and/or USP10 under chronic nutrient starvation has not yet been investigated."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al.2017); SeV Trailer RNA captures TIAR from SG (Iseni et al.2002); West Nile Virus (WNV) and Dengue virus (DENV) 3′-end viral genome captures TIA-1/TIAR (Li et al.2002; Emara and Brinton 2007; Xia et al.2015); DENV 3′-UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al.2011; Reineke et al.2015); JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al.2011)."

sparser
"ZIKV infection induces the redistribution of TIAR to the viral RNA replication sites (Hou et al. 2017) ; SeV Trailer RNA captures TIAR from SG (Iseni et al. 2002) ; West Nile Virus (WNV) and Dengue virus (DENV) 3 0 -end viral genome captures TIA-1/TIAR (Li et al. 2002; Emara and Brinton 2007; Xia et al. 2015) ; DENV 3 0 -UTR interacts with G3BP1, G3BP2, Caprin1 and USP10 (Ward et al. 2011; Reineke et al. 2015) ; JEV recruits G3BP and USP10 to the perinuclear region through the interaction of JEV core protein with Caprin-1, a SG-associated cellular factor (Ward et al. 2011) ."

sparser
"Additional experiments demonstrate that, in contrast to DDX6, the SG proteins G3BP1, G3BP2, Caprin1 and USP10 bind to the variable region (VR) in the 3' UTR."

sparser
"In addition, a proteomic analysis found that G3BP1, G3BP2, Caprin1 and USP10 interact with DENV RNA [171], and it was reported that G3BP1, G3BP2 and Caprin1 regulate the translation of ISG mRNAs, thus protecting DENV replication from IFN-mediated antiviral effects [172]."
ZEB1 affects USP10
2 | 3
2 | 3

sparser
"Regulation of the USP10-ZEB1 interaction by MEK-ERK signaling was shown whereby constitutive activation of ERK can phosphorylate USP10 at Ser236 to impair its interaction with ZEB1 and enable ZEB1 protein stabilization."

sparser
"Implications: The MEK-ERK regulated interaction of USP10 with ZEB1 can promote the proteasomal degradation of ZEB1 and thereby suppress its demonstrated ability to mediate tumor metastasis."

No evidence text available

No evidence text available

sparser
"Conversely, MEK-ERK inhibition blocked USP10 phosphorylation and enhanced the USP10-ZEB1 interaction shown to suppress ZEB1-mediated tumor cell migration and metastasis."
ZC3H12A affects TANK
| 3 2
| 3 2

reach
"Genotoxic stress also produced a TANK, MCPIP1, and USP10 complex that effected and enhanced deubiquitination of both TRAF6 (E3 Ubiquitin Protein Ligase) and NEMO, which in turn inhibited NF-kappaB activation."

sparser
"MCPIP1 forms a complex with USP10 and TANK, which mediates the deubiquitination of the TRAF6 K63 chain [76] and inhibits NF-κB activation upon DNA damage [33] ."

sparser
"Intriguingly, TANK was identified as a MCPIP1-interacting protein in a proteomic screen and we confirmed that TANK forms a complex with MCPIP1 and USP10."

reach
"Furthermore, we found that the TANK, MCPIP1, and USP10 complex also decreased TRAF6 ubiquitination in cells treated with IL-1beta or LPS."

reach
"In addition to genotoxic stress, the TANK, MCPIP1, and USP10 complex is also responsible for restraining TRAF6 ubiquitination in cells treated with LPS or IL-1beta."
USP10 affects ZEB1
2 | 3
2 | 3

sparser
"Regulation of the USP10-ZEB1 interaction by MEK-ERK signaling was shown whereby constitutive activation of ERK can phosphorylate USP10 at Ser236 to impair its interaction with ZEB1 and enable ZEB1 protein stabilization."

sparser
"Implications: The MEK-ERK regulated interaction of USP10 with ZEB1 can promote the proteasomal degradation of ZEB1 and thereby suppress its demonstrated ability to mediate tumor metastasis."

No evidence text available

No evidence text available

sparser
"Conversely, MEK-ERK inhibition blocked USP10 phosphorylation and enhanced the USP10-ZEB1 interaction shown to suppress ZEB1-mediated tumor cell migration and metastasis."
USP10 affects SH2D3C
| 2 3
| 3

sparser
"Since the binding of nsP3 and USP10 to G3BP inhibits SGs, we hypothesized that ICP8 binding might do the same."

sparser
"It was recently identified that a common canonical sequence motif, FGDF, mediates binding of the nsP3 and USP10 proteins to G3BP ."

sparser
"The model might also explain the elimination of binding of both nsP3 and USP10 to G3BP upon mutation of the glycine residue to alanine, which would restrict flexibility and thus hinder adequate positioning of the phenylalanine side-chains in the FGDF motifs."
| 2

reach
"Since the binding of nsP3 and USP10 to G3BP inhibits SGs, we hypothesized that ICP8 binding might do the same."

reach
"USP10 and nsP3 binding alters G3BP Biotin-isoxazole solubility."
USP10 affects PRKAA2
5 |
5 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
USP10 affects PRKAA1
4 1 |
USP10 binds PRKAA1.
4 |
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
USP10 deubiquitinates PRKAA1.
1 |
USP10 deubiquitinates PRKAA1. 1 / 1
1 |

"The deubiquitinase USP10 specifically removes ubiquitination on AMPKa to facilitate AMPKa phosphorylation by LKB1."
USP10 affects LPS-induced renal tubular epithelial cell injury
| 5
USP10 inhibits LPS-induced renal tubular epithelial cell injury. 5 / 5
| 5

eidos
"USP10 promotes activation of the NRF2 / HO-1 pathway through SIRT6 and attenuates LPS-induced renal tubular epithelial cell injury ."

eidos
"Collectively , these results suggest that USP10 promotes activation of the NRF2 / HO-1 pathway through SIRT6 and attenuates LPS-induced renal tubular epithelial cell injury ."

eidos
"These findings suggest that USP10 abates LPS-induced renal tubular epithelial cell injury ."

eidos
"Finally , we discovered that USP10 promoted activation of the NRF2 / HO-1 pathway through SIRT6 and attenuated LPS-induced renal tubular epithelial cell injury ."

eidos
"USP10 attenuates LPS-induced renal tubular epithelial cell injury ."
| 4 1

eidos
"It has been demonstrated that USP10 inhibits hepatic steatosis and insulin resistance via Sirt6 , in which Sirt6 represses the transcription levels of SREBP1 / SREBP2 and their target genes [ 114 ] ."

eidos
"31 Recently , a report suggested that USP10 can inhibit hepatic steatosis , insulin resistance , and inflammation through Sirt6 in nonalcoholic fatty liver disease ."

reach
"For example, USP10 expression is decreased and upregulated in hepatic steatosis models to inhibit hepatic steatosis, insulin resistance, and inflammation [7]."

eidos
"In a non-alcoholic fatty liver disease model , USP10 inhibits hepatic steatosis , insulin resistance , and the inflammatory response by interacting with SIRT6 and inhibiting its ubiquitination and degradation ( 6 ) , and USP10 inhibition also abolishes Mirt2 ( long non-coding RNA myocardial infarction associated transcript 2 ) overexpression-induced suppression of glucose production and lipogenesis in hepatocytes ( 7 ) ."

eidos
"For example , USP10 expression is decreased and upregulated in hepatic steatosis models to inhibit hepatic steatosis , insulin resistance , and inflammation [ 7 ] ."
USP10 affects HMOX1
| 5
USP10 activates HMOX1.
| 3
USP10 activates HMOX1. 3 / 3
| 3

reach
"USP10 promotes activation of the NRF2/HO-1 pathway through SIRT6 and attenuates LPS-induced renal tubular epithelial cell injury."

reach
"Finally, we discovered that USP10 promoted activation of the NRF2/HO-1 pathway through SIRT6 and attenuated LPS-induced renal tubular epithelial cell injury."

reach
"Collectively, these results suggest that USP10 promotes activation of the NRF2/HO-1 pathway through SIRT6 and attenuates LPS-induced renal tubular epithelial cell injury."
USP10 increases the amount of HMOX1.
| 2
USP10 increases the amount of HMOX1. 2 / 2
| 2

reach
"Furthermore, our results demonstrated that USP10 overexpression increased Sirt6, Nrf2 and HO-1 levels, but were reduced following Sirt6 knockdown (Fig. 6B)."

reach
"Western blot assay showed that LPS resulted in decreased Sirt6 and increased Nrf2 and HO-1 levels, whereas USP10 overexpression led to an increased expression of Sirt6, Nrf2 and HO-1 in HK-2 cells induced by LPS (Fig. 6 A)."
| 5
USP10 activates Cell Survival.
| 3
| 3

reach
"Thus, USP10 and p62 promote cell survival by reducing the amount of cytotoxic ubiquitinated protein monomers and oligomers."

reach
"The MTS results also showed that pharmacological inhibition of USP10 with Spautin-1 notably decreased the cell viability of CML cells."

reach
"Furthermore, depletion of USP10 in lung cancer cells causes decreased apoptosis and increased cell survival upon treatment with DNA damaging agents."
USP10 inhibits Cell Survival.
| 2

reach
"Their results show that the depletion of USP10 in A549 increased cell survival and decreased apoptosis through destabilizing MSH2 [32]."

reach
"Biologically, silenced USP10 in lung cancer cells contributes to the elongated cell survival and depressed apoptosis, partially through deubiquitinating the mismatch repair protein MSH2 [XREF_BIBR], or negatively modulating EIF4G1 mediated functions [XREF_BIBR]."
USP10 affects CH
| 5
USP10 activates CH. 5 / 5
| 5

reach
"In contrast, cardiac overexpression of USP10 protected against pressure overload induced maladaptive CH."

reach
"Taken together, these results show that USP10 deficiency promotes pressure overload induced CH."

reach
"Taken together, these data indicate that the activation of the Akt/GSK3beta/mTOR/p70-S6K signaling pathways appears to be essential for USP10 mediated CH."

reach
"Our observations indicate that USP10 deficiency promotes pressure overload induced CH and alleviated Ang II induced cardiomyocyte enlargement, accompanied by significant decreases in the expression of the fetal genes Anp, Bnp, and Myh7, and vice versa."

reach
"Because key regulators of CH such as p53, AMPK, and TRAF6 have been reported as downstream targets of USP10, 20, 21 we first evaluated the potential involvement of these target proteins in the CH pathogenesis induced by USP10 deficiency."
TANK affects ZC3H12A
| 3 2
| 3 2

reach
"Genotoxic stress also produced a TANK, MCPIP1, and USP10 complex that effected and enhanced deubiquitination of both TRAF6 (E3 Ubiquitin Protein Ligase) and NEMO, which in turn inhibited NF-kappaB activation."

sparser
"MCPIP1 forms a complex with USP10 and TANK, which mediates the deubiquitination of the TRAF6 K63 chain [76] and inhibits NF-κB activation upon DNA damage [33] ."

sparser
"Intriguingly, TANK was identified as a MCPIP1-interacting protein in a proteomic screen and we confirmed that TANK forms a complex with MCPIP1 and USP10."

reach
"Furthermore, we found that the TANK, MCPIP1, and USP10 complex also decreased TRAF6 ubiquitination in cells treated with IL-1beta or LPS."

reach
"In addition to genotoxic stress, the TANK, MCPIP1, and USP10 complex is also responsible for restraining TRAF6 ubiquitination in cells treated with LPS or IL-1beta."
SH2D3C affects USP10
| 2 3
| 3

sparser
"Since the binding of nsP3 and USP10 to G3BP inhibits SGs, we hypothesized that ICP8 binding might do the same."

sparser
"It was recently identified that a common canonical sequence motif, FGDF, mediates binding of the nsP3 and USP10 proteins to G3BP ."

sparser
"The model might also explain the elimination of binding of both nsP3 and USP10 to G3BP upon mutation of the glycine residue to alanine, which would restrict flexibility and thus hinder adequate positioning of the phenylalanine side-chains in the FGDF motifs."
| 2

reach
"Since the binding of nsP3 and USP10 to G3BP inhibits SGs, we hypothesized that ICP8 binding might do the same."

reach
"USP10 and nsP3 binding alters G3BP Biotin-isoxazole solubility."
PRKAA2 affects USP10
5 |
5 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
MYC affects USP10
| 4 1
MYC increases the amount of USP10.
| 3 1
MYC increases the amount of USP10. 4 / 4
| 3 1

sparser
"USP10 transcription induced by c-Myc improved the stability of the p14ARF protein [69] ."

reach
"C-Myc directly binds to the second E-box sequence of the USP10 gene and activates the transcription of USP10 [69]."

reach
"USP10 also antagonizes c-myc transcription through deubiquitination of SIRT6, a histone deacetylase, and NF-kappaB signaling through deubiquitination of NEMO [XREF_BIBR]."

reach
"MYC activates USP10 transcription by binding to the second E-box sequence located upstream of the USP10 transcription start site."
MYC activates USP10.
| 1
MYC activates USP10. 1 / 1
| 1

reach
"Our studies indicate that USP10 induced by c-Myc has a crucial role in OIS by maintaining the stability of key tumor suppressor p14ARF."
HDAC7 affects USP10
| 1 4
| 1 4

sparser
"Taken together, our results demonstrate that USP10 interacts with HDAC7 and stabilize HDAC7 protein through promoting HDAC7 deubiquitination."

sparser
"Next, to gain more insight into the USP10-HDAC7 interaction, a series of GST-tagged truncated mutants of USP10 and HA-tagged HDAC7 were co-transfected, and the C-terminal region (701–798 aa) of USP10 was determined as the binding region with HDAC7 (Fig.  xref f, g)."

sparser
"Therefore, promotion of USP10-HDAC7 axis drives ESCC cell growth."

sparser
"After screening the potential deubiquitinating enzymes of HDAC7, we first reported deubiquitinase USP10 could bind and stabilize HDAC7 protein in ESCC cells."

reach
"Furthermore, deubiquitinase USP10 interacted with and stabilized HDAC7."
FSTL1 affects USP10
| 4 1
FSTL1 binds USP10.
| 1 1
| 1 1

sparser
"The present study employed both in vivo and in vitro experiments to decipher the changes in Notch1 and USP10 and to evaluate the possible interaction between FSTL1 and USP10 in the pathological mechanism of MI-induced myocardial fibrosis in T2DM."

reach
"The present study employed both in vivo and in vitro experiments to decipher the changes in Notch1 and USP10 and to evaluate the possible interaction between FSTL1 and USP10 in the pathological mechanism of MI-induced myocardial fibrosis in T2DM."
FSTL1 activates USP10.
| 2
FSTL1 activates USP10. 2 / 2
| 2

reach
"The present study revealed that FSTL1 and USP10 were significantly activated in T2DM mice with MI, and FSTL1 treatment further increased USP10 activation and alleviating cardiac dysfunction by reducing myocardial fibrosis."

reach
"Further in vitro analysis provided evidence that FSTL1 activated USP10/Notch1 signaling to inhibit myocardial fibrosis."
FSTL1 increases the amount of USP10.
| 1
FSTL1 increases the amount of USP10. 1 / 1
| 1

reach
"Simultaneously, the data in Figure 5 revealed that the expression of Notch1 and USP10 were elevated in cardiac tissue post MI in T2DM, and further activated by FSTL1 administration."
4 |
Pirinixic acid increases the amount of USP10. 4 / 4
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
Peptide affects USP10
| 4
| 4

reach
"Peptide cellulose SPOT array with single amino-acid substitutions in the USP10 binding peptide."
| DOI

reach
"Impact of G3BP1 F33W mutation on USP10 and Caprin1 peptide binding."
| DOI

reach
"These data suggest that the contribution of F124 to USP10 peptide binding is much less than might be expected based on earlier co-crystal structures (17, 26) ."
| DOI

reach
"Previous crystal structures have demonstrated the importance of hydrophobic interactions and -stacking in the G3BP1 groove for USP10 and Caprin1 peptide binding (17, 26, 30) ."
| DOI

sparser
"Thus, the goal of this study was to test the hypothesis that Cif inhibits USP10, which increases the amount of ubiquitinated CFTR that is degraded in lysosomes, thereby reducing cell and plasma membrane CFTR level."

sparser
"To provide additional support for the hypothesis that Cif inactivation of USP10 is responsible for the increase in the amount of ubiquitinated CFTR and its lysosomal degradation, we examined the amount of multi-ubiquitinated CFTR following siRNA knockdown of USP10."

sparser
"Specifically, Cif inhibits the deubiquinating enzyme ubiquitin specific peptidase 10 (USP10), thereby reducing the USP10-mediated deubiquination of CFTR and promoting CFTR trafficking to and degradation in lysosomes [87]."
| PMC

sparser
"Cif inactivates USP10 in the early endosome."
ZC3H12A affects IKBKG
| 4
| 4

sparser
"NEMO ubiquitylation is decreased through the deubiquitinase USP10, which interacts with NEMO via MCPIP1 upon genotoxic stress."

sparser
"Moreover, usp4-depleted zebrafish (Danio rerio) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment. [102] Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) cleaves K63-linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to highrisk human papillomavirus (hrHPV) infection. [103] TRAF family member-associated NF-B activator (TANK) interacts with both monocyte chemotactic protein-1-induced protein-1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF-B activation in response to TLR activation. [104] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO-attached M1-linked polyubiquitin chains, thus inhibiting the genotoxic NF-B signaling cascade. [40] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."

sparser
"Moreover, usp4 ‐depleted zebrafish ( Danio rerio ) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment. [ xref ] Ubiquitin carboxyl‐terminal hydrolase L1 (UCHL1) cleaves K63‐linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to high‐risk human papillomavirus (hrHPV) infection. [ xref ] TRAF family member‐associated NF‐ κ B activator (TANK) interacts with both monocyte chemotactic protein‐1‐induced protein‐1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF‐ κ B activation in response to TLR activation. [ xref ] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO‐attached M1‐linked polyubiquitin chains, thus inhibiting the genotoxic NF‐ κ B signaling cascade. [ xref ] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."

sparser
"Moreover, usp4‐depleted zebrafish (Danio rerio) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment.[ 102 ] Ubiquitin carboxyl‐terminal hydrolase L1 (UCHL1) cleaves K63‐linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to high‐risk human papillomavirus (hrHPV) infection.[ 103 ] TRAF family member‐associated NF‐κB activator (TANK) interacts with both monocyte chemotactic protein‐1‐induced protein‐1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF‐κB activation in response to TLR activation.[ 104 ] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO‐attached M1‐linked polyubiquitin chains, thus inhibiting the genotoxic NF‐κB signaling cascade.[ 40 ] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."
USP7 affects NHE3
| 4
USP10 binds USP7 and NHE3. 4 / 4
| 4

sparser
"We showed that FSK attenuated the interaction of USP7 and USP10 with NHE3, resulting in increased ubiquitination of NHE3."

sparser
"NHE3 is inhibited by protein kinase A. Activation of PKA by forskolin decreased the binding of USP7 and USP10 to NHE3, while increasing ubiquitination of NHE3."

sparser
"The binding of USP7 and USP10 to NHE3 was confirmed by co-immunoprecipitation."

sparser
"To confirm that USP7 or USP10 interact with NHE3, we performed immunoprecipitation of NHE3 using P5D4 or IgG as a negative control, followed by immunoblotting for USP7 or USP10."
USP10 affects peptide
| 4
| 4

reach
"Peptide cellulose SPOT array with single amino-acid substitutions in the USP10 binding peptide."
| DOI

reach
"Impact of G3BP1 F33W mutation on USP10 and Caprin1 peptide binding."
| DOI

reach
"These data suggest that the contribution of F124 to USP10 peptide binding is much less than might be expected based on earlier co-crystal structures (17, 26) ."
| DOI

reach
"Previous crystal structures have demonstrated the importance of hydrophobic interactions and -stacking in the G3BP1 groove for USP10 and Caprin1 peptide binding (17, 26, 30) ."
| DOI
USP10 affects c-Myc oncogene
| 4
USP10 inhibits c-Myc oncogene. 4 / 4
| 4

reach
"30 In addition, previous studies have demonstrated that USP10 antagonizes the transcriptional activity of the c-Myc oncogene through Sirt6 to inhibit cell cycle progression, cell growth, and tumor formation in human colon cancers."

reach
"They also reported that USP10 antagonized the transcriptional activity of the c-Myc oncogene through SIRT6, as well as p53, thereby inhibiting cell cycle progression, cancer cell growth, and tumoral f[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Because one of the molecular mechanisms by which SIRT6 achieves its tumor suppressive functions is through c-Myc inhibition, our results suggest that USP10 inhibits the c-Myc oncogene, at least partially, through SIRT6 stabilization."

reach
"USP10 antagonizes the transcriptional activity of the c-Myc oncogene through SIRT6, as well as p53, to inhibit cell cycle progression, cancer cell growth, and tumor formation."
USP10 affects TRAF6
1 | 3
USP10 deubiquitinates TRAF6. 4 / 4
1 | 3

"We found TANK formed a complex with MCPIP1 (also known as ZC3H12A) and a deubiquitinase, USP10, which was essential for the USP10-dependent deubiquitination of TRAF6 and the resolution of genotoxic NF-κB activation upon DNA damage."

reach
"The USP10-dependent deubiquitination of NEMO and TRAF6 proteins is another strategy through which MCPIP1 can regulate inflammation and the immune response [34]."

reach
"We found TANK formed a complex with MCPIP1 (also known as ZC3H12A) and a deubiquitinase, USP10, which was essential for the USP10 dependent deubiquitination of TRAF6 and the resolution of genotoxic NF-kappaB activation upon DNA damage."

reach
"TRAF Family Member associated NF-kappaB Activator (TANK) Inhibits Genotoxic Nuclear Factor kappaB Activation by Facilitating Deubiquitinase USP10 dependent Deubiquitination of TRAF6 Ligase."
USP10 affects TBX21
1 | 3
1 | 3

sparser
"Stability of T-bet has been shown to be positively regulated by USP10, which directly interacts with T-bet to mediate its deubiquitination [ xref ]."

No evidence text available

sparser
"Our results revealed that USP10 could interact with T-bet and stabilize it via deubiquitination to inhibit its degradation by the proteosome."

sparser
"Our results show that USP10 interacts with T-bet and mediates the deubiquitination of T-bet, stabilizes and increases the expression of T-bet."
USP10 affects PIK3C3
| 4
USP10 activates PIK3C3.
| 2
USP10 activates PIK3C3. 2 / 2
| 2

reach
"We propose that USP10 and USP13 mediate the deubiqutination of Vps34 complexes to regulate the levels of class III PI3 kinase."

reach
"Interestingly, Spautin-1-mediated specific and potent inhibition of deubiquitinating activity of USP10 and USP13 was shown to promote proteasomal degradation of PIK3C3 and thereby inhibit autophagy [179]."
USP10 inhibits PIK3C3.
| 1
USP10 inhibits PIK3C3. 1 / 1
| 1

reach
"USP10 and USP13, two ubiquitin‐specific peptidases, can target the Beclin1 subunit of Vps34‐Beclin1 and promote the degradation of the Vps34 complex."
USP10 binds PIK3C3.
| 1
| 1

reach
"Consistent with this possibility, the effect of spautin-1 on the levels of USP10 and Vps34 complexes can be fully restored in the presence of MG132 (XREF_SUPPLEMENTARY)."
USP10 affects PABPC1
2 | 2
2 |

No evidence text available

No evidence text available
| 2

sparser
"Finally, acetylation of Lysine-376 (K376) in the RRM domain, impairs the RNA binding function of G3BP1 as well as its interaction with PABP1, USP10 or Caprin1[ xref ]."

sparser
"As previously discussed, acetylation of K376 of G3BP1 by the CBP/P300 acetylase is a key modification impeding SGs assembly by impairing its RNA binding function, as well as its interaction with PABP1, USP10 or Caprin1[ xref ]."
USP10 affects NHE3
| 4
USP10 binds USP7 and NHE3. 4 / 4
| 4

sparser
"We showed that FSK attenuated the interaction of USP7 and USP10 with NHE3, resulting in increased ubiquitination of NHE3."

sparser
"NHE3 is inhibited by protein kinase A. Activation of PKA by forskolin decreased the binding of USP7 and USP10 to NHE3, while increasing ubiquitination of NHE3."

sparser
"The binding of USP7 and USP10 to NHE3 was confirmed by co-immunoprecipitation."

sparser
"To confirm that USP7 or USP10 interact with NHE3, we performed immunoprecipitation of NHE3 using P5D4 or IgG as a negative control, followed by immunoblotting for USP7 or USP10."
USP10 affects LC3B
| 4
USP10 increases the amount of LC3B.
| 2
USP10 increases the amount of LC3B. 2 / 2
| 2

reach
"The ubiquitin isopeptidase USP10 deubiquitinates LC3B to increase LC3B levels and autophagic activity."

reach
"Biochemical analyses showed that silencing of USP10 reduces the levels of both the LC3B-I and LC3B-II forms of LC3B through increased ubiquitination and proteasomal degradation."
USP10 deubiquitinates LC3B.
| 2
USP10 deubiquitinates LC3B. 2 / 2
| 2

reach
"The ubiquitin isopeptidase USP10 deubiquitinates LC3B to increase LC3B levels and autophagic activity."

reach
"Herein, we report that LC3B ubiquitination is reversed by the action of the deubiquitinating enzyme USP10."
USP10 affects His2Av
| 4
USP10 deubiquitinates His2Av.
| 3
USP10 deubiquitinates His2Av. 3 / 3
| 3

reach
"USP10 specifically deubiquitylates H2A.Z and H2A."

reach
"In addition, the deubiquitination of H2A.Z by USP10 is required for the androgen-receptor mediated transcriptional activation of hormone regulated genes [XREF_BIBR]."

reach
"USP10 directly deubiquitylates H2A.Z in vitro and in vivo, and reducing USP10 expression in prostate cancer cells results in elevated steady-state levels of mono-ubiquitylated H2A.Z (H2A.Zub1)."
USP10 decreases the amount of His2Av.
| 1
USP10 decreases the amount of His2Av. 1 / 1
| 1

reach
"Finally, knockdown of USP10 also impairs the loss of H2A.Z at the regulatory regions of PSA and KLK2 that normally occurs during their transcriptional activation."
USP10 affects EIF2AK2
| 4
| 4

sparser
"Validation of the mass spectrometry data confirmed the interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."

sparser
"NCAP showed specific interaction with the SG proteins G3BP1, G3BP2, YTHDF3, USP10 and PKR, and translocated to SGs following oxidative stress and heat shock."

sparser
"NCAP interacts with SG proteins. (A) Interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."

sparser
"Fig. 4: NCAP interacts with SG proteins. (A) Interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."
| 4
USP10 inhibits Carcinogenesis.
| 2

reach
"Wang et al. reported that the loss of USP10 promotes lung tumorigenesis and progression in mice; meanwhile, reduced expression of USP10 is associated with the poor prognosis of patient suffering from lung cancer clinically [46]."

reach
"USP10 is a critical regulator of KLF4 stability and suppresses lung tumorigenesis [19]."
USP10 activates Carcinogenesis.
| 2

reach
"Although some upstream regulators and substrates of USP10 have been identified, in-depth investigations of both the underlying mechanisms of USP10-mediated tumorigenesis and the development of corresponding drugs are urgently needed."

reach
"The research by Yuan et al. demonstrated that USP10 inhibits cancer cell proliferation in wild-type p53 cells, but promotes tumorigenesis in a mutant p53 background (Yuan et al., 2010)."
USP10 affects CCND3
| 2 2
USP10 inhibits CCND3.
| 1 1
USP10 inhibits CCND3. 2 / 2
| 1 1

reach
"Furthermore, USP10 specifically prevented CCND3 from K48-linked polyubiquitination and proteasomal degradation, therefore enhancing its activity."

eidos
"Furthermore , USP10 specifically prevented CCND3 from K48-linked polyubiquitination and proteasomal degradation , therefore enhancing its activity ."
USP10 activates CCND3.
| 1 1
USP10 activates CCND3. 2 / 2
| 1 1

reach
"By stabilizing CCND3, USP10 activated the CCND3/CDK4/6 signaling pathway, phosphorylated Rb, and upregulated CDK4, CDK6 and E2F-1 in OPM2 and KMS11 cells."

eidos
"By stabilizing CCND3 , USP10 activated the CCND3 / CDK4 / 6 signaling pathway , phosphorylated Rb , and upregulated CDK4 , CDK6 and E2F-1 in OPM2 and KMS11 cells ."
USP10 affects ANLN
| 2 2
USP10 inhibits ANLN.
| 2
USP10 inhibits ANLN. 2 / 2
| 2

reach
"USP10 removes the K11- and K63-linked ubiquitin chains of ANLN through its deubiquitinase activity and prevents ANLN ubiquitin-mediated degradation."

reach
"Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma."
USP10 binds ANLN.
| 2
| 2

sparser
"Furthermore, the findings of the study showed that the deubiquitinating enzyme USP10 interacts with ANLN and positively regulates ANLN protein levels."

sparser
"Overall, targeting the USP10-ANLN axis can effectively inhibit ESCC cell-cycle progression."
USP10 affects AKT
| 4
USP10 inhibits AKT.
| 2
USP10 inhibits AKT. 2 / 2
| 2

reach
"USP10 was reported to inhibit tumor growth and inactivate mTORC1 and AKT signaling by stabilizing AMPKalpha and PTEN in HCC cells."

reach
"Specifically, USP10 activates PTEN by preventing its K63 linked polyubiquitination mediated by TRIM25 and suppresses the AKT and mTOR signaling pathway thereby inhibiting NSCLC proliferation, indicating that it may be a potential drug target for cancer treatment."
USP10 dephosphorylates AKT.
| 2
USP10 leads to the dephosphorylation of AKT. 2 / 2
| 2

reach
"Collectively, our results uncover an undescribed mechanism where USP10, as a tumor suppressor, negatively regulates mTORC1 activation and AKT phosphorylation by stabilizing AMPKalpha and PTEN in HCC cells."

reach
"Additionally, the MK2206 and rapamycin significantly suppressed AKT phosphorylation and mTOR complex1 activation in USP10 knockdowned HCC cells, may indicate that AKT inhibitor and rapamycin can be co[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
TOP2alpha affects USP10
| 4
USP10 binds TOP2alpha. 4 / 4
| 4

reach
"In this study, we also report that in addition to RNF168, TOP2alpha also interacts with USP10."

reach
"Given that our mass spectrometry analysis identified USP10 as a possible RNF168 interacting partner (XREF_SUPPLEMENTARY), we hypothesized that USP10 and RNF168 forms a complex with TOP2alpha to co-regulate its ubiquitylation level."

reach
"These novel functional interactions between RNF168, TOP2alpha and USP10 are likely to contribute to the maintenance of chromosomal integrity."

reach
"Finally, we show that the deubiquitylase USP10 interacts with RNF168 and TOP2alpha, and restrains ubiquitylation of TOP2alpha as well as its chromatin binding."
TBX21 affects USP10
1 | 3
1 | 3

sparser
"Stability of T-bet has been shown to be positively regulated by USP10, which directly interacts with T-bet to mediate its deubiquitination [ xref ]."

No evidence text available

sparser
"Our results revealed that USP10 could interact with T-bet and stabilize it via deubiquitination to inhibit its degradation by the proteosome."

sparser
"Our results show that USP10 interacts with T-bet and mediates the deubiquitination of T-bet, stabilizes and increases the expression of T-bet."
SLC9A3 affects USP10
| 4
SLC9A3 binds USP10.
| 3
| 3

reach
"Using an activity-based chemical screening, we identified ubiquitin specific protease-7 (USP7) and USP10 that bind NHE3."

reach
"In summary, we have demonstrated that USP7 and USP10 physically interact and ubiquitinate NHE3."

reach
"The binding of USP7 and USP10 to NHE3 was confirmed by co-immunoprecipitation."
SLC9A3 inhibits USP10.
| 1
SLC9A3 inhibits USP10. 1 / 1
| 1

reach
"These results collectively demonstrate that knockdown of USP7 or USP10 increases NHE3 trafficking from the surface membrane to the late endosomes."
PRKAA1 affects USP10
4 |
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
PABPC1 affects USP10
2 | 2
2 |

No evidence text available

No evidence text available
| 2

sparser
"Finally, acetylation of Lysine-376 (K376) in the RRM domain, impairs the RNA binding function of G3BP1 as well as its interaction with PABP1, USP10 or Caprin1[ xref ]."

sparser
"As previously discussed, acetylation of K376 of G3BP1 by the CBP/P300 acetylase is a key modification impeding SGs assembly by impairing its RNA binding function, as well as its interaction with PABP1, USP10 or Caprin1[ xref ]."
NHE3 affects USP7
| 4
USP10 binds USP7 and NHE3. 4 / 4
| 4

sparser
"We showed that FSK attenuated the interaction of USP7 and USP10 with NHE3, resulting in increased ubiquitination of NHE3."

sparser
"NHE3 is inhibited by protein kinase A. Activation of PKA by forskolin decreased the binding of USP7 and USP10 to NHE3, while increasing ubiquitination of NHE3."

sparser
"The binding of USP7 and USP10 to NHE3 was confirmed by co-immunoprecipitation."

sparser
"To confirm that USP7 or USP10 interact with NHE3, we performed immunoprecipitation of NHE3 using P5D4 or IgG as a negative control, followed by immunoblotting for USP7 or USP10."
IKBKG affects ZC3H12A
| 4
| 4

sparser
"NEMO ubiquitylation is decreased through the deubiquitinase USP10, which interacts with NEMO via MCPIP1 upon genotoxic stress."

sparser
"Moreover, usp4-depleted zebrafish (Danio rerio) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment. [102] Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) cleaves K63-linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to highrisk human papillomavirus (hrHPV) infection. [103] TRAF family member-associated NF-B activator (TANK) interacts with both monocyte chemotactic protein-1-induced protein-1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF-B activation in response to TLR activation. [104] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO-attached M1-linked polyubiquitin chains, thus inhibiting the genotoxic NF-B signaling cascade. [40] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."

sparser
"Moreover, usp4 ‐depleted zebrafish ( Danio rerio ) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment. [ xref ] Ubiquitin carboxyl‐terminal hydrolase L1 (UCHL1) cleaves K63‐linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to high‐risk human papillomavirus (hrHPV) infection. [ xref ] TRAF family member‐associated NF‐ κ B activator (TANK) interacts with both monocyte chemotactic protein‐1‐induced protein‐1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF‐ κ B activation in response to TLR activation. [ xref ] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO‐attached M1‐linked polyubiquitin chains, thus inhibiting the genotoxic NF‐ κ B signaling cascade. [ xref ] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."

sparser
"Moreover, usp4‐depleted zebrafish (Danio rerio) larvae have been shown to produce more proinflammatory cytokines and were more susceptible to endotoxic challenge following LPS treatment.[ 102 ] Ubiquitin carboxyl‐terminal hydrolase L1 (UCHL1) cleaves K63‐linked polyubiquitin chains on TRAF3, which ultimately leads to reduced production of type I IFN, proinflammatory cytokines, and chemokines in response to high‐risk human papillomavirus (hrHPV) infection.[ 103 ] TRAF family member‐associated NF‐κB activator (TANK) interacts with both monocyte chemotactic protein‐1‐induced protein‐1 (MCPIP1) and USP10, leading to the cleavage of ubiquitin chains on TRAF6 and termination of NF‐κB activation in response to TLR activation.[ 104 ] Of note, USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO‐attached M1‐linked polyubiquitin chains, thus inhibiting the genotoxic NF‐κB signaling cascade.[ 40 ] In addition to interacting with USP10, MCPIP1 itself regulates cellular inflammation pathways with distinct mechanisms."
G3BP2 affects EIF2AK2
| 4
| 4

sparser
"Validation of the mass spectrometry data confirmed the interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."

sparser
"NCAP showed specific interaction with the SG proteins G3BP1, G3BP2, YTHDF3, USP10 and PKR, and translocated to SGs following oxidative stress and heat shock."

sparser
"NCAP interacts with SG proteins. (A) Interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."

sparser
"Fig. 4: NCAP interacts with SG proteins. (A) Interaction of NCAP with G3BP1, G3BP2, YTHDF3, USP10 and PKR."
AKT affects USP10
| 1 3
AKT phosphorylates USP10. 2 / 2
| 2

sparser
"In addition, the in vitro kinase assay confirmed that AKT directly phosphorylates wild-type USP10 peptide, but not T674A USP10 peptide (Supplementary Fig. xref )."

sparser
"In addition, the in vitro kinase assay confirmed that AKT directly phosphorylates wild-type USP10 peptide, but not T674A USP10 peptide (Supplementary Fig. 4c )."
AKT phosphorylates USP10 on T674. 2 / 2
| 1 1

reach
"In addition, Luo et al. reported that co-stimulation by BCR and TLR1/2 initiated the AKT-dependent phosphorylation of T674 of USP10; subsequently, USP10 entered the nucleus and stabilized the AID protein (also see Figure 4 and Table 2) [72]."

sparser
"In addition, Luo et al. reported that co-stimulation by BCR and TLR1/2 initiated the AKT-dependent phosphorylation of T674 of USP10; subsequently, USP10 entered the nucleus and stabilized the AID protein (also see Figure 4 and Table 2 ) [72] ."
Vasopressin affects USP10
| 3
| 3

reach
"In mCCD (cl1) cells, vasopressin increases SNX3 protein but not mRNA, supporting the idea that the vasopressin induced Usp10 deubiquitylates and stabilizes endogenous SNX3 and consequently promotes cell surface expression of ENaC."

reach
"For example, vasopressin can induce USP10 (ubiquitin specific protease) in mouse cortical collecting duct cells and its interaction with USP10 deubiquitinates sorting nexin 3 results in stabilization [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"We have identified a mRNA encoding the deubiquitylating enzyme ubiquitin specific protease 10 (Usp10), whose expression is increased by vasopressin at both the mRNA and the protein level."
MiR-138 affects USP10
| 3
MiR-138 inhibits USP10. 3 / 3
| 3

reach
"However, overexpression of miR-138, miR-191 and miR-34a-5p inhibits the mRNA and protein expression levels of USP10."

reach
"We further found miR-138 overexpression inhibits TP53-dependent transcription by repressing USP10 and abrogates TP53-dependent cell apoptosis and cell cycle arrest."

reach
"The overexpression of miR-138 inhibits the mRNA and protein expression levels of USP10 (also see Figure 4 and Table 2)."
Bisphenol A affects USP10
3 |
Bisphenol A increases the amount of USP10.
2 |
Bisphenol A increases the amount of USP10. 2 / 2
2 |

No evidence text available

No evidence text available
Bisphenol A decreases the amount of USP10.
1 |
Bisphenol A decreases the amount of USP10. 1 / 1
1 |

No evidence text available
Anti-μ antibody affects USP10
| 3
Anti-μ antibody leads to the phosphorylation of USP10 on T674. 3 / 3
| 3

reach
"To further clarify that anti-μ antibody and Pam3Csk4 co-treatment promoted T674 phosphorylation of USP10, we first generated an antibody that specifically recognizes the phosphorylation site of USP10 by immunizing rabbits with the phosphorylated and sequence-homologous polypeptides (Supplementary Fig. 4a)."

reach
"To further clarify that anti-μ antibody and Pam 3 Csk 4 co-treatment promoted T674 phosphorylation of USP10, we first generated an antibody that specifically recognizes the phosphorylation site of USP10 by ."

reach
"Western blot analysis of Ramos B cells and mouse spleen B cells showed that the co-stimulation of anti-μ antibody and Pam3Csk4 promoted USP10 T674 phosphorylation, but not when AKT inhibitor was used (Fig. 6i, j)."
USP7 affects TP53
| 3
TP53 binds USP10 and USP7. 3 / 3
| 3

sparser
"For example, USP7 and USP10 bind to and deubiquitylate their substrate p53 to mediate its role for suppression of cell propagation upon cellular stresses by counteracting its degradation xref – xref ."

sparser
"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced under DMOG treatment ( xref A)."

sparser
"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced un[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP10 affects transport
| 3
| 3

reach
"These results suggested that USP10 functions to stimulate transport of p62 aggregates to the perinuclear aggresome formation site."

reach
"In addition, USP10 stimulates the transport of multiple p62-positive ubiquitinated protein aggregates to the perinuclear region to form one big aggregate (aggresome)."

reach
"USP10, by interacting with p62, further augments p62-induced ubiquitinated protein aggregation and then stimulates the transport of p62/ubiquitinated protein aggregates to the perinuclear aggresome-forming site."

reach
"Deubiquitinating enzyme USP10 promotes osteosarcoma metastasis and epithelial-mesenchymal transition by stabilizing YAP1."

reach
"Functional assays revealed that USP10 knockdown suppressed cell migrating and invading ability and inhibited the EMT of OS cells in vivo and in vitro."

reach
"As shown in Fig. 5A, depletion of USP10 in Bel‐7402 cells significantly decreased the expression of EMT marker proteins (N‐cadherin, vimentin, and Slug)."

reach
"Among them, G3BP1/2 is regulated by the phosphorylation site of S149 and may bind to caprin1 or USP10; binding to caprin1 may promote the formation of SGs, while binding to USP10 may inhibit the formation of SGs (23) ."

reach
"The overexpression of USP10 can inhibit the formation of stress granules (SGs), thus restraining the development of a tumor [19,24]."

reach
"For example, inhibiting USP10 reduces the production of reactive oxygen species and suppresses apoptosis and the formation of SGs (stress granules) [78]."
| 3

reach
"Ouchida et al. (2018) identified that USP10 functions as a deubiquitinase and regulator of the EMT‐transcription factor Slug to promote cell migration in multiple tumor cells, including non‐small‐cell lung carcinoma, ovarian cancer, fibrosarcoma, and breast cancer."

reach
"Interesting, Spautin‐1 imposed minimal effect on the cell proliferation (Fig. S5C), suggesting that under our experimental conditions, USP10 mainly modulated cell migration rather than cell proliferation, through the regulatory effect on Smad4."

reach
"Figure 5B displays that depletion of endogenous USP10 with shRNAs markedly decreased cell migration compared to that of the control cells, as indicated by the inhibition rate on wound healing as 56.15%, 59.19%, and 76.42%, respectively (P < 0.001)."
USP10 affects activity
| 3
USP10 inhibits activity. 3 / 3
| 3

sparser
"To further dissect how USP10 inhibits mTOR activity, we investigated whether the stabilization of PTEN [] and AMPKα [] by USP10 involved the physical interactions of USP10 with PTEN and AMPKα."

sparser
"Thus, USP10 and p62 might inhibit proteasome activity by trapping proteasome components in protein aggregates and/or aggresomes."

sparser
"USP10 inhibits c-Myc transcriptional activity."
USP10 affects Ubiquitin
| 3
| 3

reach
"USP10 removes the K11- and K63-linked ubiquitin chains of ANLN through its deubiquitinase activity and prevents ANLN ubiquitin-mediated degradation."

reach
"We found that ubiquitin specific peptidase 10 (USP10) interacted with the NOTCH1 intracellular domain (NICD1) to slow the ubiquitin dependent turnover of this short lived form of the activated NOTCH1 receptor."

reach
"Silencing of the DUB USP10 induces a buildup of ubiquitin on integrins beta1 and beta5 in cell lysates, whereas recombinant USP10 removes ubiquitin from these integrin subunits."
USP10 affects TRAF4
| 1 2
| 1 2

sparser
"TRAF4 interacts with the deubiquitinase USP10 and blocks the access of tumor protein P53 (TP53) to USP10, destabilizing TP53 [ xref ]."

reach
"Specifically, TRAF4 interacted with the deubiquitinase USP10 and blocked the access of p53 to USP10, resulting in p53 destabilization."

sparser
"It is also revealed that TRAF4 interacts with the deubiquitinating enzymes USP10, blocks the binding of p53 to USP10 and induce p53 instability [ xref , xref ]."
USP10 affects SOX21
| 2 1
USP10 binds SOX21.
| 1 1
| 1 1

reach
"Also, the binding of SOX21 and USP10 was also verified by RIP assay (Fig. 4G)."

sparser
"Also, the binding of SOX21 and USP10 was also verified by RIP assay (Fig.  xref G)."
USP10 inhibits SOX21.
| 1
USP10 inhibits SOX21. 1 / 1
| 1

reach
"We could see that USP10 down-regulation inhibited the half-life of SOX21 protein (Fig. 4H, I), but this phenomenon was reversed after MG132 treatment (Fig. 4J)."
USP10 affects SNX3
1 1 1 |
USP10 binds SNX3.
1 1 |
1 1 |

No evidence text available

No evidence text available
USP10 deubiquitinates SNX3.
1 |
USP10 deubiquitinates SNX3. 1 / 1
1 |

"?A two-hybrid screen identified sorting nexin 3 (SNX3) as a novel substrate of Usp10."
USP10 affects RUNX1
| 1 2
USP10 activates RUNX1. 3 / 3
| 1 2

eidos
"Conversely , USP10 knockdown inhibited RUNX1 and resulted in the loss of MES properties ."

reach
"Conversely, USP10 knockdown inhibited RUNX1 and resulted in the loss of MES properties."

reach
"Overexpression of USP10 upregulated RUNX1 and induced proneural-to-mesenchymal transition (PMT), thus maintaining MES properties in GBM."
USP10 affects INS
| 3
USP10 inhibits INS. 3 / 3
| 3

reach
"Ubiquitin Specific Peptidase 10 (USP10) Inhibits Hepatic Steatosis, Insulin Resistance, and Inflammation Through Sirt6."

reach
"31 Recently, a report suggested that USP10 can inhibit hepatic steatosis, insulin resistance, and inflammation through Sirt6 in nonalcoholic fatty liver disease."

reach
"USP10 inhibits hepatic steatosis, insulin resistance and inflammation via Sirt6."
USP10 affects H2Aub1
| 3
USP10 deubiquitinates H2Aub1. 3 / 3
| 3

reach
"AR-related proteins including H2A, Zub1, and H2Aub1 can be deubiquitinated and stabilized by USP10."

reach
"Indeed, in this study, we found that USP10 deubiquitylates H2A.Zub1 and H2Aub1 in vitro and in vivo."

reach
"Co-transfection studies showed that USP10 specifically deubiquitylates H2A.Zub1 and H2Aub1, but not H2Bub1, in a dose dependent manner."
USP10 affects G3BP1/2
| 3
USP10 binds G3BP1/2. 3 / 3
| 3

reach
"Among them, G3BP1/2 is regulated by the phosphorylation site of S149 and may bind to caprin1 or USP10; binding to caprin1 may promote the formation of SGs, while binding to USP10 may inhibit the formation of SGs (23) ."

reach
"This FGDF motif is also found in SFV and USP10, which can also bind to G3BP1/2 to block the assembly of SGs (82)."

reach
"We now show that (a) deletion of G3BP1/2 ablates SGs triggered by p-eIF2alpha or eIF4A inhibition, but not those induced by hyperosmolarity or severe heat shock, (b) the phosphomimetic G3BP1-S149E fails to rescue SG competence, (c) binding of G3BP to Caprin1 or USP10 is not required for SG competence, but may alter G3BP and USP10 protein levels, (d) Caprin1 and USP10 bind G3BP1/2 in a mutually exclusive manner with antagonistic effects on SGs, (e) G3BP1 interacts with 40S ribosomal subunits through its RGG motif, which is required for SG competence, (f) overexpression of USP10 inhibits SG formation downstream of polysome disassembly, and (g) USP10 and FGDF binding to G3BP specifically alters the B-isox precipitation of G3BP1/2 and Caprin1, but not that of other SG associated proteins."
USP10 affects Foam Cells
| 3
| 3

eidos
"Significantly , USP10 promotes foam cell formation ."

eidos
"USP10 inhibition or deletion diminishes the formation of foam cell ."

eidos
"What is more , the formation of foam cell is inhibited by USP10 inhibitor or siRNA in RAW264.7 and THP1 cells ."
USP10 affects Flag
| 3
| 3

sparser
"To determine whether ubiquitinated NP is specifically deubiquitinated by USP11, we co-transfected plasmids that can express NP-HA, Myc-Ub and Flag-USP10 or USP11, and then subjected HA-tagged immunoprecipitates to immunoblot analysis using the indicated antibodies ( Figure 5D )."

sparser
"Moreover, ectopic expression of Flag-USP10 resulted in dose-dependent elevation of HA-HDAC7 in HEK-293T cells (Fig.  xref b), whereas USP10 knockdown markedly increased HDAC7 polyubiquitination and decreased the HDAC7 protein expression without influencing the HDAC7 mRNA levels in EC109 cells (Fig.  xref c, d)."

sparser
"The NP-HA plasmid was replaced by PA-HA or PB2-HA, and visualized by immunoblot with anti-HA and anti-myc antibodies. (D) 293T cells were co-transfected with plasmids that can express NP-HA, Flag-USP10, Flag-USP11 and Myc-Ub as indicated."
USP10 affects Fibrosis
| 2 1
| 2 1

eidos
"USP10 ameliorated histological steatosis , inflammation , and fibrosis ."

eidos
"USP10 could promote autophagy-related pathways and molecules and increase the synthesis of autophagosomes in NASH , improving steatosis , inflammation , and fibrosis ."

reach
"USP10 could promote autophagy-related pathways and molecules and increase the synthesis of autophagosomes in NASH, improving steatosis, inflammation, and fibrosis."
USP10 affects FUT1
| 3
USP10 inhibits FUT1. 3 / 3
| 3

sparser
"However, our results using USP10 functional mutants indicated that ROS inhibition by USP10 is dispensable for USP10 to inhibit apoptosis of HSCs."

sparser
"Since aberrant ROS production has been shown to be a critical apoptosis inducer of HSCs under basal and stress conditions ( xref ), it was initially assumed that USP10 inhibits apoptosis of HSCs by inhibiting ROS production."

sparser
"These results suggest that inhibition of HSC apoptosis is a function of USP10 to maintain the number of HSCs in FL, and that USP10 selectively inhibits apoptosis of HSCs, MPPs, and HPCs but not more differentiated LCPs."
USP10 affects FOXO4
| 3
USP10 increases the amount of FOXO4. 3 / 3
| 3

reach
"In addition, FoxO4 modulated USP10 transcription."

reach
"In conclusion, FoxO4 negatively modulated USP10 transcription to aggravate the apoptosis and oxidative stress of H/R induced H9C2 cells via blocking Hippo and YAP pathway."

reach
"FoxO4 negatively modulates USP10 transcription to aggravate the apoptosis and oxidative stress of hypoxia and reoxygenation induced cardiomyocytes by regulating the Hippo and YAP pathway."
USP10 affects CDKN1A
| 2
USP10 activates CDKN1A. 2 / 3
| 2

reach
"As shown in XREF_FIG, overexpression of WT USP10 increased p21 promoter activity in HCT116 p53 +/+ cells, but not in HCT116 p53 -/- cells."

reach
"Stable knockdown of USP10 by shRNA inhibited p21 promoter activity in HCT116 p53 +/+ cells, but had little effect in HCT116 p53 -/- cells (XREF_FIG)."
USP10 affects CCND1
| 1 2
| 1 2

sparser
"In this study, Sun et al. found that USP10 may interact with CCND1 and prevent its K48-linked polyubiquitination, increasing CCND1 stability."

sparser
"USP10 interacted with CCND1 and prevented its K48- but not K63-linked polyubiquitination in GBM U251 and HS683 cells, which led to increased CCND1 stability."

reach
"USP10 interacted with CCND1 and prevented its K48- but not K63 linked polyubiquitination in GBM U251 and HS683 cells, which led to increased CCND1 stability."
USP10 affects Beclin1 subunit
| 3
USP10 activates Beclin1 subunit. 3 / 3
| 3

reach
"Spautin-1 promotes the degradation of Vps34 PI3 kinase complexes by inhibiting two ubiquitin specific peptidases, USP10 and USP13, that target the Beclin1 subunit of Vps34 complexes."

reach
"Our second treatment was with Spautin-1 which promotes the degradation of Vps34 PI3 kinase complexes by inhibiting two ubiquitin specific peptidases, USP10 and USP13, which target the Beclin1 subunit of Vps34 complexes."

reach
"We treated TBEV infected neuroblastoma cells with rapamycin (inducer of autophagy, as inhibition of mTOR mimics cellular starvation by blocking signals required for cell growth and proliferation), and spautin-1 (highly specific and potent autophagy inhibitor in mammalian cells, which promotes the degradation of Vps34 PI3 kinase complexes by inhibiting two ubiquitin specific peptidases, USP10 and USP13 that target the Beclin1 subunit of Vps34 complexes) and investigated the effect of the treatment of TBEV growth."
TRAF4 affects USP10
| 1 2
| 1 2

sparser
"TRAF4 interacts with the deubiquitinase USP10 and blocks the access of tumor protein P53 (TP53) to USP10, destabilizing TP53 [ xref ]."

reach
"Specifically, TRAF4 interacted with the deubiquitinase USP10 and blocked the access of p53 to USP10, resulting in p53 destabilization."

sparser
"It is also revealed that TRAF4 interacts with the deubiquitinating enzymes USP10, blocks the binding of p53 to USP10 and induce p53 instability [ xref , xref ]."
TP53 affects USP7
| 3
TP53 binds USP10 and USP7. 3 / 3
| 3

sparser
"For example, USP7 and USP10 bind to and deubiquitylate their substrate p53 to mediate its role for suppression of cell propagation upon cellular stresses by counteracting its degradation xref – xref ."

sparser
"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced under DMOG treatment ( xref A)."

sparser
"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced un[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
TBP affects USP10
1 | 1
TBP binds USP10.
1 |
1 |

No evidence text available
TBP activates USP10.
| 1
TBP activates USP10. 1 / 1
| 1

reach
"Importantly, both western blot and flow cytometry analysis confirmed modestly increased TBP protein levels in a C2C12 stable cell line overexpressing USP10 (XREF_FIG, XREF_FIG)."
SH2D3C affects G3BP1
| 3
| 3

sparser
"Since the binding of nsP3 and USP10 to G3BP inhibits SGs, we hypothesized that ICP8 binding might do the same."

sparser
"It was recently identified that a common canonical sequence motif, FGDF, mediates binding of the nsP3 and USP10 proteins to G3BP ."

sparser
"The model might also explain the elimination of binding of both nsP3 and USP10 to G3BP upon mutation of the glycine residue to alanine, which would restrict flexibility and thus hinder adequate positioning of the phenylalanine side-chains in the FGDF motifs."
Receptors, Antigen, B-Cell leads to the phosphorylation of USP10 on T674. 3 / 3
| 3

reach
"I, j Western blot indicated TLR1/2 and BCR co-stimulation promoted USP10 T674 phosphorylation, but not when AKT inhibitor treatment."

reach
"Collectively, these results indicated that BCR and TLR2 synergistically enhance Akt activity and promote USP10 T674 phosphorylation located within the NLS domain and, therefore, the nuclear import of USP10, consequently inhibiting the AID degradation in the nucleus.Pam3Csk4 has been used as a vaccine adjuvant.59 To explore the effect of Pam3Csk4 on the SARS-CoV-2 RBD particle vaccine immune response, USP10-BWT and USP10-BKO mice were immunized with adjuvants in combination with the SARS-CoV-2 RBD particle vaccine."

reach
"Collectively, these results indicated that BCR and TLR2 synergistically enhance Akt activity and promote USP10 T674 phosphorylation located within the NLS domain and, therefore, the nuclear import of USP10, consequently inhibiting the AID degradation in the nucleus.Pam 3 Csk 4 helped to enhance the antibody response to the SARS-CoV-2 RBD nanoparticle vaccine Pam 3 Csk 4 has been used as a vaccine adjuvant."
PTEN affects AMPK_alpha
| 3
| 3

sparser
"Therefore, we conclude that USP10 interacts with PTEN and AMPKα, inhibits their polyubiquitylation, and as a result stabilizes PTEN and AMPKα."

sparser
"To further dissect how USP10 inhibits mTOR activity, we investigated whether the stabilization of PTEN [] and AMPKα [] by USP10 involved the physical interactions of USP10 with PTEN and AMPKα."

sparser
"Inversely, endogenous PTEN and AMPKα interacted with endogenous USP10 in HCC-LM3 and HUH7 cells (B)."
MSH2 affects USP10
2 | 1
2 | 1

No evidence text available

sparser
"However, the clinicopathological features and survival analysis indicated that the USP10 and MSH2 proteins were not associated with clinical features, including age, sex, tumor size, Tumor-Node-Metastasis stage and tumor cell differentiation, along with the prognosis of NSCLC."

No evidence text available
HBX19818 affects USP10
| 1 2
HBX19818 inhibits USP10. 3 / 3
| 1 2

reach
"Notably, P22077, HBX19818, and HBX19818 analogs including Compounds 3, 7, and 9 also inhibit USP10 in mutant-FLT3-expressing cells."

reach
"However, a recent study (Weisberg et al., 2017) revealed that P22077 and HBX19818 may also inhibit the deubiquitinase activity of USP10 and induce the proliferation inhibition of mutant-FLT3 (FLT3-ITD)-positive cancer cells."

eidos
"Along with USP7 , P22077 and HBX19818 has also been reported to inhibit USP10 , promoting degradation of FLT3-mutant AML cells [ 174 ] ."
G3BP1/2 affects USP10
| 3
USP10 binds G3BP1/2. 3 / 3
| 3

reach
"Among them, G3BP1/2 is regulated by the phosphorylation site of S149 and may bind to caprin1 or USP10; binding to caprin1 may promote the formation of SGs, while binding to USP10 may inhibit the formation of SGs (23) ."

reach
"This FGDF motif is also found in SFV and USP10, which can also bind to G3BP1/2 to block the assembly of SGs (82)."

reach
"We now show that (a) deletion of G3BP1/2 ablates SGs triggered by p-eIF2alpha or eIF4A inhibition, but not those induced by hyperosmolarity or severe heat shock, (b) the phosphomimetic G3BP1-S149E fails to rescue SG competence, (c) binding of G3BP to Caprin1 or USP10 is not required for SG competence, but may alter G3BP and USP10 protein levels, (d) Caprin1 and USP10 bind G3BP1/2 in a mutually exclusive manner with antagonistic effects on SGs, (e) G3BP1 interacts with 40S ribosomal subunits through its RGG motif, which is required for SG competence, (f) overexpression of USP10 inhibits SG formation downstream of polysome disassembly, and (g) USP10 and FGDF binding to G3BP specifically alters the B-isox precipitation of G3BP1/2 and Caprin1, but not that of other SG associated proteins."
G3BP1 affects SH2D3C, and USP10
| 3
| 3

sparser
"Since the binding of nsP3 and USP10 to G3BP inhibits SGs, we hypothesized that ICP8 binding might do the same."

sparser
"It was recently identified that a common canonical sequence motif, FGDF, mediates binding of the nsP3 and USP10 proteins to G3BP ."

sparser
"The model might also explain the elimination of binding of both nsP3 and USP10 to G3BP upon mutation of the glycine residue to alanine, which would restrict flexibility and thus hinder adequate positioning of the phenylalanine side-chains in the FGDF motifs."
Flag affects USP10
| 3
| 3

sparser
"To determine whether ubiquitinated NP is specifically deubiquitinated by USP11, we co-transfected plasmids that can express NP-HA, Myc-Ub and Flag-USP10 or USP11, and then subjected HA-tagged immunoprecipitates to immunoblot analysis using the indicated antibodies ( Figure 5D )."

sparser
"Moreover, ectopic expression of Flag-USP10 resulted in dose-dependent elevation of HA-HDAC7 in HEK-293T cells (Fig.  xref b), whereas USP10 knockdown markedly increased HDAC7 polyubiquitination and decreased the HDAC7 protein expression without influencing the HDAC7 mRNA levels in EC109 cells (Fig.  xref c, d)."

sparser
"The NP-HA plasmid was replaced by PA-HA or PB2-HA, and visualized by immunoblot with anti-HA and anti-myc antibodies. (D) 293T cells were co-transfected with plasmids that can express NP-HA, Flag-USP10, Flag-USP11 and Myc-Ub as indicated."
CCND1 affects USP10
| 1 2
| 1 2

sparser
"In this study, Sun et al. found that USP10 may interact with CCND1 and prevent its K48-linked polyubiquitination, increasing CCND1 stability."

sparser
"USP10 interacted with CCND1 and prevented its K48- but not K63-linked polyubiquitination in GBM U251 and HS683 cells, which led to increased CCND1 stability."

reach
"USP10 interacted with CCND1 and prevented its K48- but not K63 linked polyubiquitination in GBM U251 and HS683 cells, which led to increased CCND1 stability."
AMPK_alpha affects PTEN, and USP10
| 3
| 3

sparser
"Therefore, we conclude that USP10 interacts with PTEN and AMPKα, inhibits their polyubiquitylation, and as a result stabilizes PTEN and AMPKα."

sparser
"To further dissect how USP10 inhibits mTOR activity, we investigated whether the stabilization of PTEN [] and AMPKα [] by USP10 involved the physical interactions of USP10 with PTEN and AMPKα."

sparser
"Inversely, endogenous PTEN and AMPKα interacted with endogenous USP10 in HCC-LM3 and HUH7 cells (B)."
Yki affects USP10
| 2
USP10 binds yki. 2 / 2
| 2

sparser
"In summary, our results provided strong evidence that Usp10 binds to Yki and regulates Yki activity and function through modulating the ubiquitination level and subsequent protein turnover."

sparser
"USP10 binds to Yki and promotes Yki deubiquitination and stabilization through the proteasome ubiquitination pathway, while Usp10 may regulate human YAP activity [48] ."
Tetrachloromethane increases the amount of USP10. 2 / 2
2 |

No evidence text available

No evidence text available
Resveratrol affects USP10
| 1
| 1

reach
"As expected, the interaction between G3BP1 and USP10 decreased in HCT116 cells treated with resveratrol (XREF_FIG)."
P53-P359A affects USP10
| 2
USP10 binds p53-P359A. 2 / 2
| 2

reach
"Furthermore, P359A mutation reduced the interaction with USP10 in comparison to p53 wt and the interaction between the p53-P359A and USP10 was not regulated by DMOG."

reach
"Furthermore, P359A mutation reduced the interaction with USP10 in comparison to p53 wt and the interaction between the p53-P359A and USP10 was not regulated by DMOG (XREF_FIG D)."
MiR-34a-5p affects USP10
| 2
MiR-34a-5p inhibits USP10. 2 / 2
| 2

reach
"However, overexpression of miR-138, miR-191 and miR-34a-5p inhibits the mRNA and protein expression levels of USP10."

reach
"In an investigation for the Mirt2 associated molecular mechanism, it has been emphasized that miR-34a-5p is considered the target of Mirt2, and miR-34a-5p is a repressor of USP10."
| 2
| 2

reach
"Treatment of cells with LMB induced USP10 accumulation in the nucleus, suggesting that USP10 is actively exported out of nucleus in unstressed cells."

reach
"LMB treatment also induced USP10 nuclear localization, suggesting that USP10 is actively exported out of the nuclear in unstressed cells."
Indometacin affects USP10
2 |
Indometacin decreases the amount of USP10. 2 / 2
2 |

No evidence text available

No evidence text available

reach
"In this study, we identified spautin-1, an autophagy inhibitor that suppresses ubiquitin-specific peptidase 10 (USP10) and USP13, as a novel UPR inhibitor under glucose starvation conditions."

reach
"Another autophagy inhibitor, spautin-1, degrades class III PI3K by inhibiting the activity of the ubiquitin specific peptidases, USP10 and USP13 (Mateo et al., 2013, Liu et al., 2011)."
Act2 affects USP10
| 2
| 2

reach
"Using recombinant proteins, we found that USP10 directly interacted with AMPK alpha1 and alpha2 but not the other AMPK subunits (XREF_FIG)."

reach
"Using recombinant proteins, we found that USP10 directly interacted with AMPK alpha1 and alpha2 but not the other AMPK subunits."
Wu-5 affects USP10
| 2
Wu-5 inhibits USP10. 2 / 2
| 2

reach
"Mechanistically, Wu-5 inhibits USP10 activity through interacting with USP10 in cells."

reach
"For example, USP10 regulates Notch signaling pathway in the endothelium [23]; USP10 protects against cerebral ischemia injury by attenuating inflammation and apoptosis through inhibiting the TAK1 signaling pathway [8]; Interaction of USP10 and G3BP2 blocks p53 signaling and results in poor prognosis in prostate cancer [24]; Wu-5, which is an inhibitor of USP10, enhances crenolanib-induced FLT3-ITD-positive AML cell death by suppressing FLT3 and AMPK signaling pathways [25]."
2 |
Vehicle Emissions increases the amount of USP10. 2 / 2
2 |

No evidence text available

No evidence text available
USP13 affects BECN1
| 2
| 2

sparser
"To determine the mechanism by which Vps34 complexes regulate the stability of USP13 and USP10, we examined the possibility that Beclin1 may interact with USP10 and USP13."

sparser
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."
USP10 affects yki
| 2
USP10 binds yki. 2 / 2
| 2

sparser
"In summary, our results provided strong evidence that Usp10 binds to Yki and regulates Yki activity and function through modulating the ubiquitination level and subsequent protein turnover."

sparser
"USP10 binds to Yki and promotes Yki deubiquitination and stabilization through the proteasome ubiquitination pathway, while Usp10 may regulate human YAP activity [48] ."
USP10 affects xenograft growth
| 2
USP10 activates xenograft growth. 2 / 2
| 2

eidos
"Depletion or inhibition of USP10 reduces xenograft growth and sensitizes xenografts to cisplatin The data presented up to this point prompted us to test whether depletion of USP10 in cancers lacking wild-type p53 decreases tumorigenesis and increases chemosensitivity in vivo ."

eidos
"Knockdown of USP10 drastically reduced xenograft growth , and in combination with cisplatin treatment almost completely abolished tumor initiation ."
USP10 affects tumor growth
| 2
USP10 inhibits tumor growth. 2 / 2
| 2

eidos
"Furthermore , USP10 can also interact with and deubiquitinate PTEN , USP10 inhibition stimulates tumor growth and invasion , but this effect can be abolished by reinserting PTEN ( Sun et al ., 2018 ) ."

eidos
"USP10 was reported to inhibit tumor growth and inactivate mTORC1 / AKT signaling by stabilizing AMPKalpha and PTEN in HCC cells ( 12 ) ."
USP10 affects tioguanine
| 2
| 2

reach
"However, it seems to contradict the finding that USP10 promotes sensitivity to 6-TG by stabilizing MSH2 41."

reach
"When we planned to continue our 2016 study 41, we noticed that USP10 only promotes sensitivity to 6-TG in A549, a wild-type p53 cell line, but not in H1299, a null-p53 cell line."
USP10 affects stability
| 2
USP10 activates stability. 2 / 2
| 2

eidos
"For example , USP7 regulates the stability of both p53 and Mdm2 and maintains p53 ubiquitination levels ; 120 USP2 mediates the stability of Mdm2 ; 121 USP10 modulates p53 localization and stability ; 122 OTUB1 abrogates p53 ubiquitination and activates p53.123 Interestingly , USP10 can stabilize both mutated and wild-type p53 , with a dual role in tumorigenesis ."

eidos
"Accordingly , inactivation of USP10 reduced Sirt6 abundance and stability and diminished Sirt6-induced downstream signaling in cardiomyocytes ."
USP10 affects spautin-1
| 2
USP10 binds spautin-1. 2 / 2
| 2

reach
"However, there is still no evidence showing that spautin-1 or spautin-A41 directly binds to USP10."

reach
"No orthogonal biophysical methods, such as the surface plasmon response (SPR) or isothermal titration calorimetry (ITC), were used to validate the binding of spautin-1 with USP10 or USP13."
USP10 affects renal dysfunction induced
| 2
USP10 activates renal dysfunction induced. 2 / 2
| 2

eidos
"Based on these findings , we inferred that USP10 alleviates renal dysfunction induced by CLP ."

eidos
"USP10 alleviates renal dysfunction induced by CLP ."
USP10 affects pressure overload-induced CH
| 2
USP10 inhibits pressure overload-induced CH. 2 / 2
| 2

eidos
"Taken together , these results show that USP10 deficiency promotes pressure overload-induced CH ."

eidos
"Our observations indicate that USP10 deficiency promotes pressure overload-induced CH and alleviated Ang II-induced cardiomyocyte enlargement , accompanied by significant decreases in the expression of the fetal genes Anp , Bnp , and Myh7 , and vice versa ."
USP10 affects pathway
| 2
USP10 activates pathway. 2 / 2
| 2

sparser
"Moreover, USP10 activates the Raf-1/MEK/ERK pathway, which is an important regulator of EMT [60] ."

sparser
"Taken together, these results demonstrate that USP10 activates TGF‐β pathway by specifically stabilizing Smad4 protein in HCC models."
USP10 affects pTau aggregation
| 2
USP10 activates pTau aggregation. 2 / 2
| 2

eidos
"Therefore , the present study suggested that USP10 and TIA1 cooperatively promote pTau aggregation in AD through SG formation ."

eidos
"These results supported the notion that USP10 promotes pTau aggregation through SG formation in AD patients ."
USP10 affects p53-P359A
| 2
USP10 binds p53-P359A. 2 / 2
| 2

reach
"Furthermore, P359A mutation reduced the interaction with USP10 in comparison to p53 wt and the interaction between the p53-P359A and USP10 was not regulated by DMOG."

reach
"Furthermore, P359A mutation reduced the interaction with USP10 in comparison to p53 wt and the interaction between the p53-P359A and USP10 was not regulated by DMOG (XREF_FIG D)."
USP10 affects p14ARF
| 2
USP10 increases the amount of p14ARF. 2 / 2
| 2

isi
"Prognostic significance of USP10 and p14ARF expression in patients with colorectal cancer."

isi
"USP10 and p14ARF protein expression was assessed via immunohistochemistry (IHC) on a tissue microarray from 280 colorectal cancer cases."
USP10 affects malignant process
| 2
USP10 activates malignant process. 2 / 2
| 2

eidos
"USP10 accelerates the CRC malignant process by upregulating NLRP7 ."

eidos
"USP10 accelerates the CRC malignant process by upregulating NLRP7 To explore the role of NLRP7 in the tumor-promoting function of USP10 , the effect of NLRP7 overexpression on the tumorigenic potential of USP10-shRNA lentivirus-stable-transfected HCT116 and SW480 cells was examined ."
USP10 affects mUb-PCNA
| 2
USP10 deubiquitinates mUb-PCNA. 2 / 2
| 2

reach
"Although Poleta reduced affinity for the DNA beyond the CPD might contribute to its dissociation after TLS 28, deubiquitination of mUb-PCNA by USP1 29 or USP10 30 can promote Poleta displacement from replication forks."

reach
"Moreover, deubiquitination of mUb-PCNA by USP1 29, USP10 30, or interferon stimulated gene 15 modification of PCNA has also been suggested to dictate TLS termination 30."
| 2
| 2

reach
"USP10 modulates anterograde and retrograde vesicular trafficking, the localization and stability of p53 and binds Dengue virus RNA."

reach
"Usp10 inhibition attenuated H 2 O 2 -induced senescence in MLO-Y4 cells, as indicated by p53 and p21 quantification, a senescence associated beta-galactosidase (SA-beta-gal) assay, and p53 localization visualization with a confocal microscope."
USP10 affects lipid uptake macrophage
| 2
USP10 activates lipid uptake macrophage. 2 / 2
| 2

eidos
"USP10 mediates lipid uptake by macrophage ."

eidos
"To further verify whether USP10 mediates lipid uptake by macrophage , we overexpressed USP10 to evaluate lipid uptake using Dil-oxLDL ."
USP10 affects lactate
| 2
USP10 activates lactate. 2 / 2
| 2

reach
"Expression of USP10 shRNA increased lipid droplet content, lactate production and glycolytic gene expression in control cells but not in AMPKalpha deficient cells (XREF_SUPPLEMENTARY), indicating that USP10 regulates cellular metabolism through AMPK."

reach
"Expression of USP10 shRNA increased lipid droplet content, lactate production, and glycolytic gene expression in control cells but not in AMPKalpha deficient cells, indicating that USP10 regulates cel[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP10 affects inflammation apoptosis
| 2
USP10 inhibits inflammation apoptosis. 2 / 2
| 2

eidos
"USP10 inhibit inflammation and apoptosis in hepatocytes after H / R treatment ."

eidos
"Taken together , these results demonstrated that USP10 inhibited inflammation and apoptosis in hepatocytes after H / R treatment ."
USP10 affects hepatocyte inflammation
| 2
USP10 inhibits hepatocyte inflammation. 2 / 2
| 2

eidos
"Consistently , in our study , we also demonstrated that USP10 inhibited hepatocyte inflammation and apoptosis in hepatocytes following H / R stimulation ( Figure 5 ) ."

eidos
"In this study , we also demonstrated that USP10 inhibited hepatocyte inflammation and apoptosis , depending on TAK1 phosphorylation , and JNK and p38 activation ( Figure 6 ) ."

eidos
"For instance , USP10 and USP21 could promote hepatocellular carcinoma and non-small-cell lung cancer proliferation , respectively [ 36 , 37 ] ."

eidos
"USP10 depletion enhanced the polyubiquitination of YAP / TAZ , promoted their proteasomal degradation , and ultimately arrested the proliferation of hepatocellular carcinoma in vitro and in vivo [ 38 ] ."
USP10 affects hepatic R injury
| 2
USP10 inhibits hepatic R injury. 2 / 2
| 2

eidos
"Therefore , we investigated whether USP10 deficiency promotes hepatic I / R injury in a MAPK-dependent manner ."

eidos
"These results demonstrate that USP10 knockdown aggravates hepatic I / R injury ."
USP10 affects growth
| 1
USP10 inhibits growth. 1 / 2
| 1

sparser
"Downregulation of p53 abolished the inhibitory effect of USP10 on tumor growth ( xref and data not shown), confirming that USP10 inhibits cancer cell growth by stabilizing p53."
USP10 affects glucose
| 2
| 2

reach
"Furthermore, Usp10 knockout mice showed increased blood glucose level and decreased glucose infusion rate, indicating USP10 regulates hepatic glucose metabolism."

reach
"Furthermore, Usp10 knockout mice showed increased blood glucose level and decreased glucose infusion rate (XREF_FIG), indicating USP10 regulates hepatic glucose metabolism."
USP10 affects formation
| 2
USP10 inhibits formation. 2 / 2
| 2

sparser
"USP10 inhibits SG formation downstream of polysome disassembly."

sparser
"Nancy presented preliminary data indicating differential regulation of SG nucleation by these G3BP1 binding partners, whereby Caprin1 and USP10 bind the G3BP-NTF2-like domain in a competitive manner, with opposite results; G3BP1:Caprin1 complexes promote SG formation, whereas G3BP1:USP10 complexes inhibit SG formation."
USP10 affects degradation
| 2
USP10 inhibits degradation. 2 / 2
| 2

sparser
"These results suggested that LC3 is degraded by autophagy in both USP10-WT and USP10-KD cells with or without MG-132 treatment, and that USP10-KD does not inhibit autophagy-mediated degradation of LC3."

sparser
"Notably, genetic or pharmacological inhibition of USP10 using Spautin-1 prominently decreased the stability of endogenous SKP2 protein (Fig.  xref ), suggesting that USP10 inhibits the degradation of SKP2 in vivo."
USP10 affects cisplatin
| 2
| 2

reach
"As we further tested the role of USP10 in cisplatin sensitivity in a large panel of NSCLC cell lines, surprisingly, we discovered that upon cisplatin treatment the depletion or inhibition of USP10 could lead to two entirely different outcomes: cell survival or apoptosis, depending on the cellular status of TP53—USP10 promotes cisplatin sensitivity in wild-type p53 cells, while USP10 confers cisplatin resistance in mutant p53 cells."

reach
"In addition, Spain-1, an inhibitor of USP10, inhibits melanoma growth and improves the anticancer effect of cisplatin by inhibiting USP10 activity [87,88]."
USP10 affects cholesterol
| 2
| 2

reach
"Knockout of Usp10 significantly increased hepatic triglyceride and cholesterol contents, as measured by a colorimetric assay and HE staining under both normal fat diet and high fat diet condition (XREF_FIG)."

reach
"Knockout of Usp10 significantly increased hepatic triglyceride and cholesterol contents, as measured by a colorimetric assay and H&E staining under both normal-fat diet and high-fat diet conditions."
USP10 affects cell cycle
| 2
| 2

reach
"Furthermore, USP10 knockdown mediated cell cycle progression was partially rescued by either SIRT6 or p53 co-expression."

reach
"Third, USP10 antagonizes c-Myc transcriptional activity and cell cycle progression through both SIRT6 and p53."
USP10 affects cardiac hypertrophy
| 2
USP10 inhibits cardiac hypertrophy. 2 / 2
| 2

eidos
"USP10 deficiency promotes cardiac hypertrophy ."

eidos
"Collectively , these data demonstrate that USP10 can interact with Sirt6 and promote the ubiquitination of Sirt6 in cardiomyocytes and then inhibit the downstream Akt signaling pathway and cardiac hypertrophy ."
USP10 affects assembly
| 2
USP10 inhibits assembly. 2 / 2
| 2

sparser
"Because both USP10 overexpression and G3BP deletion similarly prevent SG formation, the data suggest that USP10 inhibits G3BP-mediated SG assembly downstream of translational arrest/polysome disassembly."

sparser
"Interestingly, this same N-terminal pocket bound by resveratrol has also been shown to interact with the FGDF domains in USP10 and the Alphavirus protein NSP3 [ xref ], which inhibit SG assembly by competing with CAPRIN1 binding."

reach
"Importantly, USP10 promotes contractile ring assembly at the cytokinetic furrow as well as cytokinesis by stabilizing ANLN."

eidos
"Importantly , USP10 promotes contractile ring assembly at the cytokinetic furrow as well as cytokinesis by stabilizing ANLN ."
USP10 affects activation
| 2
USP10 inhibits activation. 2 / 2
| 2

sparser
"These results indicate that USP10 may inhibit mTOR activation through regulating PTEN/AKT/PI3K and AMPK signaling pathways in HCC cells."

sparser
"For example, USP10 can inhibit genotoxic NF-κB activation through monocyte chemotactic protein-1-induced protein-1 (MCPIP1)-facilitated deubiquitination of NEMO [ xref ]."
USP10 affects activation TAK1-JNK p38 signalling
| 2
USP10 inhibits activation TAK1-JNK p38 signalling. 2 / 2
| 2

eidos
"Mechanistically , USP10 inhibited the activation of TAK1-JNK / p38 signalling , and the detrimental role of USP10 deficiency in hepatic I / R injury was blocked by inhibition of TAK1 activation in hepatocytes ."

eidos
"Together , these observations suggest that USP10 inhibited the activation of TAK1-JNK / p38 signalling during hepatic I / R injury ."
USP10 affects activation NRF2 HO-1 pathway
| 2
USP10 activates activation NRF2 HO-1 pathway. 2 / 2
| 2

eidos
"Finally , we discovered that USP10 promoted activation of the NRF2 / HO-1 pathway through SIRT6 and attenuated LPS-induced renal tubular epithelial cell injury ."

eidos
"Collectively , these results suggest that USP10 promotes activation of the NRF2 / HO-1 pathway through SIRT6 and attenuates LPS-induced renal tubular epithelial cell injury ."
USP10 affects act2
| 2
| 2

reach
"Using recombinant proteins, we found that USP10 directly interacted with AMPK alpha1 and alpha2 but not the other AMPK subunits (XREF_FIG)."

reach
"Using recombinant proteins, we found that USP10 directly interacted with AMPK alpha1 and alpha2 but not the other AMPK subunits."
| 2

eidos
"USP10 decreases the ubiquitination of CFTR , whereas the knockdown of USP10 promotes the ubiquitination and reduction of CFTR at the cell surface [ 85 ] ."
| PMC

eidos
"USP10 interacts with SIRT6 to inhibit its ubiquitination and degradation , thereby preventing fatty liver and cardiac hypertrophy [ 7 ] ."
USP10 affects UBC
2 |
2 |

No evidence text available

No evidence text available
USP10 affects TGF‐β
| 2
USP10 activates TGF‐β. 2 / 2
| 2

reach
"The current study identified the prometastatic roles of USP10 in HCC and found that USP10 promotes TGF‐β signaling and HCC metastasis by stabilizing Smad4 through the cleavage of proteolytic K48‐linked ubiquitination.5 Conclusions."

reach
"Further study demonstrated that USP10 promotes TGF‐β signaling and HCC metastasis through the regulation on the protein abundance of Smad4, but imposing minimal effect on Smad2 and Smad3."
USP10 affects TAFAZZIN
| 2
USP10 deubiquitinates TAFAZZIN. 2 / 2
| 2

reach
"In the context of HCC, USP10 promotes HCC cell proliferation and metastasis by deubiquitinating and stabilizing YAP and TAZ, the effector transcription factors of the Hippo signaling pathway, and SMAD4, the major signaling effector of TGFβ signaling [39,40,]."

reach
"USP10 depletion enhanced the polyubiquitination of YAP/TAZ, promoted their proteasomal degradation, and ultimately arrested the proliferation of hepatocellular carcinoma in vitro and in vivo [38]."
USP10 affects SNCA
| 1 1
USP10 increases the amount of SNCA.
| 1
Modified USP10 increases the amount of SNCA. 1 / 1
| 1

reach
"Like CFTR-DeltaF508, coexpression of USP10 with GFP-alpha-synuclein and synphilin-1 increased the amounts of insoluble alpha-synuclein and insoluble ubiquitinated proteins (XREF_FIG D)."
USP10 binds SNCA.
| 1
| 1

sparser
"In cultured cells, USP10 promoted α-synuclein-induced aggresome formation ( xref A) and USP10 was often detected at the periphery of α-synuclein-positive aggresomes ( xref C and xref C)."
USP10 affects SNAI2
| 1 1
| 1 1

sparser
"USP10 interacts with Slug and mediates its degradation by the proteasome."

reach
"USP10 interacts with Slug and mediates its degradation by the proteasome."
USP10 affects SCNN1A
1 1 |
1 1 |

No evidence text available

No evidence text available
USP10 affects RPS3
1 | 1
USP10 deubiquitinates RPS3. 2 / 2
1 | 1

No evidence text available

reach
"Subsequent deubiquitination of RPS2 and RPS3 by USP10 is critical for recycling of stalled ribosomes in a process known as ribosome associated quality control."
USP10 affects RPL7A
| 2
RPL7A binds USP10, PARP1, and NCL. 2 / 2
| 2

sparser
"Independent co-IP experiments using FLAG-tagged TARG1 from cell lysates in the presence of olaparib confirmed binding to ARTD1, NCL, RPL7A, and USP10 (Fig.  xref )."

sparser
"Independent co-IP experiments using FLAG-tagged TARG1 from cell lysates in the presence of olaparib confirmed binding to ARTD1, NCL, RPL7A, and USP10 (Fig. 1f)."
USP10 affects Phosphatase
| 1 1
| 1 1

eidos
"In contrast to TRIM25 , USP10 restored PTEN phosphatase activity and reduced the production of the secondary messenger PI ( 3,4,5 ) P3 , thereby inhibiting AKT / mTOR pro-growth signaling transduction in NSCLC cells ."

reach
"In contrast to TRIM25, USP10 restored PTEN phosphatase activity and reduced the production of the secondary messenger PI (3,4,5) P3, thereby inhibiting AKT and mTOR pro growth signaling transduction in NSCLC cells."
USP10 affects PPP6R1
2 |
2 |

No evidence text available

No evidence text available
USP10 affects PMT
| 2
USP10 activates PMT. 2 / 2
| 2

reach
"USP10 maintains MES properties of GBM, and promotes PMT of GBM cells."

reach
"Overexpression of USP10 upregulated RUNX1 and induced proneural-to-mesenchymal transition (PMT), thus maintaining MES properties in GBM."
USP10 affects PGK1
| 2
| 2

reach
"Together, our study demonstrated for the first time that KIF15 enhances the glycolytic capacity of PC through the recruitment of USP10 and PGK1, and that the KIF15/USP10/PGK1 axis may serve as an effective therapeutic agent for PC."

reach
"The MS and Co-IP assay indicated that KIF15 recruited and enhanced the binding between PGK1 and USP10."
USP10 affects PARP1
| 2
RPL7A binds USP10, PARP1, and NCL. 2 / 2
| 2

sparser
"Independent co-IP experiments using FLAG-tagged TARG1 from cell lysates in the presence of olaparib confirmed binding to ARTD1, NCL, RPL7A, and USP10 (Fig.  xref )."

sparser
"Independent co-IP experiments using FLAG-tagged TARG1 from cell lysates in the presence of olaparib confirmed binding to ARTD1, NCL, RPL7A, and USP10 (Fig. 1f)."
| 2

eidos
"Moreover , USP10 was found to reduce oxidative stress , and abated LPS-induced renal tubular epithelial cell injury and apoptosis ."

eidos
"USP10 alleviates sepsis-induced renal dysfunction and reduces renal tubular epithelial cell apoptosis and oxidative stress ."
USP10 affects Notch
| 2
USP10 inhibits Notch. 2 / 2
| 2

reach
"Inactivation of USP10 can diminish Notch induced target gene expression in endothelial cells."

reach
"Accordingly, inactivation of USP10 reduced NICD1 abundance and stability and diminished Notch induced target gene expression in ECs."
USP10 affects NUTF2
| 2
| 2

reach
"USP10 's interaction with G3BP1 involves an FGDF motif within USP10 that binds a pocket within the N-terminal NTF2 like domain of G3BP1, and this binding inhibits SG condensation in response to some stresses [XREF_BIBR, XREF_BIBR]."

reach
"We wondered whether this interaction displaces USP10, a cellular binding partner of G3BP that also binds to the NTF2 like domain [XREF_BIBR, XREF_BIBR]."
USP10 affects NLRP7 protein
| 2
USP10 activates NLRP7 protein. 2 / 2
| 2

eidos
"a USP10 upregulation increased NLRP7 protein levels , but ( b ) had no significant effect on mRNA levels of NLRP7 in CRC cells ."

eidos
"USP10 upregulation increased NLRP7 protein levels ( Fig. 3a ) ."
USP10 affects NCL
| 2
RPL7A binds USP10, PARP1, and NCL. 2 / 2
| 2

sparser
"Independent co-IP experiments using FLAG-tagged TARG1 from cell lysates in the presence of olaparib confirmed binding to ARTD1, NCL, RPL7A, and USP10 (Fig.  xref )."

sparser
"Independent co-IP experiments using FLAG-tagged TARG1 from cell lysates in the presence of olaparib confirmed binding to ARTD1, NCL, RPL7A, and USP10 (Fig. 1f)."
USP10 affects MSI2
| 2
USP10 increases the amount of MSI2. 2 / 2
| 2

reach
"XREF_BIBR In colon cancer, USP10 positively regulates the expression of MSI2 by de-ubiquitination."

reach
"We investigated whether USP10 positively regulates the expression of MSI2 by deubiquitination and confirmed the type of polyubiquitin chain that is linked to MSI2."
USP10 affects LC3B-I
| 2
USP10 increases the amount of LC3B-I. 2 / 2
| 2

reach
"Biochemical analyses showed that silencing of USP10 reduces the levels of both the LC3B-I and LC3B-II forms of LC3B through increased ubiquitination and proteasomal degradation."

reach
"Stable expression of MYC-USP10 in USP10-KO cells restored levels of LC3B-I and LC3B-II to those in WT cells (XREF_FIG, E and F)."
USP10 affects KLHDC3
2 |
2 |

No evidence text available

No evidence text available
USP10 affects Hepatitis
| 2
| 2

eidos
"USP10 Knockdown Aggravates Liver Inflammation During Hepatic I / R Injury Inflammation response is the key mechanism of hepatic I / R injury ( 17 ) ."

eidos
"These observations demonstrate that USP10 knockdown aggravates liver inflammation during hepatic I / R injury ."
USP10 affects HSP90
| 2
USP10 inhibits HSP90. 2 / 2
| 2

sparser
"USP10 inhibits cytokine deprivation-induced apoptosis of HSPCs in vitro, and this inhibition requires the deubiquitinase activity of USP10."

sparser
"These results suggest that USP10 inhibits apoptosis of HSPCs by deubiquitinating molecule(s) other than the ones analyzed here."
USP10 affects HDAC6 protein
| 2
USP10 activates HDAC6 protein. 2 / 2
| 2

eidos
"The above results suggest that USP10 up-regulates HDAC6 protein expression , most likely through deubiquitinating and consequently stabilizing HDAC6 ."

eidos
"As shown in Fig.3a , knockdown of USP10 decreased HDAC6 protein levels as compared to the control H23 cells , while MG132 restored HDAC6 expression in the knockdown cells , indicating that blocking proteasome degradation leads to HDAC6 accumulation ."
USP10 affects H2A.Zub1
| 2
USP10 deubiquitinates H2A.Zub1. 2 / 2
| 2

reach
"Indeed, in this study, we found that USP10 deubiquitylates H2A.Zub1 and H2Aub1 in vitro and in vivo."

reach
"Co-transfection studies showed that USP10 specifically deubiquitylates H2A.Zub1 and H2Aub1, but not H2Bub1, in a dose dependent manner."
USP10 affects GST
| 2
| 2

sparser
"As shown in Fig. xref , bacterially-purified GST-USP10 and His-HDAC6 were able to interact with each other under cell-free conditions via a GST pull-down assay, suggesting a direct interaction between USP10 and HDAC6."

sparser
"To determine whether USP10 binds to HDAC6 directly or via other proteins, we generated and purified recombinant GST-USP10 and His-HDAC6 in E. coli ."
USP10 affects FlaG
| 2
| 2

sparser
"We observed that addition of His6-USP10, but not His6-USP13, reduced the amount of HA-Ub-conjugated FLAG-LC3B ( xref , C and D ), consistent with USP10 specifically catalyzing the deubiquitination of LC3B. Finally, we found that GFP-tagged LC3B (GFP-LC3B) co-immunoprecipitated with FLAG-USP10 but not FLAG-USP13 ( xref E )."

sparser
"Cells were transfected with FLAG-USP10 (green) and we found that FLAG-tagged USP10 appeared to be co-localized with endogenous CD36 ( xref )."
USP10 affects FSTL1
| 1 1
| 1 1

sparser
"The present study employed both in vivo and in vitro experiments to decipher the changes in Notch1 and USP10 and to evaluate the possible interaction between FSTL1 and USP10 in the pathological mechanism of MI-induced myocardial fibrosis in T2DM."

reach
"The present study employed both in vivo and in vitro experiments to decipher the changes in Notch1 and USP10 and to evaluate the possible interaction between FSTL1 and USP10 in the pathological mechanism of MI-induced myocardial fibrosis in T2DM."
USP10 affects ENaC
| 2
USP10 deubiquitinates ENaC. 2 / 2
| 2

reach
"USP10, in particular, deubiquitinates ENaC and CFTR increasing their expression in cell membrane."

reach
"Yet, overexpressed USP10 does not deubiquitinate the bulk of the ENaC pool."
USP10 affects EGFP
| 2
| 2

sparser
"Following induction of EGFPUSP10(1-40) or control EGFP protein expression, we treated these cells with UV or sodium arsenite, and analysed GCN2 and eIF2α phosphorylation using western blotting ( xref E) and SG formation using immunofluorescence staining ( xref F)."

sparser
"By contrast, SG formation in response to both treatments was blocked in EGFP-USP10(1-40)-expressing cells ( xref F)."
USP10 affects DNA Damage
| 1
| 1

reach
"We identify 9 direct binding partners of p53 in the nuclear RNA interactome, including USP10, which deubiquitinates and activates p53 in response to DNA damage, and Sumo1, which can itself become covalently coupled to p53 to regulate its activity and subcellular localization (XREF_SUPPLEMENTARY) XREF_BIBR XREF_BIBR XREF_BIBR."
USP10 affects DENV-2 RNA
| 2
USP10 binds DENV-2 RNA. 2 / 2
| 2

reach
"In addition, a quantitative mass spectrometry study revealed that DENV-2 RNA interacts with the SG components G3BP-1/2, Caprin1, and USP10."

reach
"In addition, a quantitative mass spectrometry study revealed that DENV-2 RNA interacts with the SG components G3BP-1/2, Caprin1, and USP10 (Ward et al., 2011)."
USP10 affects CRC
| 2
USP10 activates CRC. 2 / 2
| 2

reach
"USP10 accelerates the CRC malignant process by upregulating NLRP7."

reach
"Targeting USP10 by shRNA inhibited CRC proliferation and metastasis, and this effect was reversed by overexpression of NLRP7."
USP10 affects CLP-induced oxidative stress renal tissues
| 2
USP10 inhibits CLP-induced oxidative stress renal tissues. 2 / 2
| 2

eidos
"USP10 attenuates CLP-induced oxidative stress in renal tissues ."

eidos
"Altogether , these results illustrate that USP10 attenuates CLP-induced oxidative stress in renal tissues ."
USP10 affects CLP-induced apoptosis renal tissues
| 2
USP10 inhibits CLP-induced apoptosis renal tissues. 2 / 2
| 2

eidos
"USP10 reduces CLP-induced apoptosis in renal tissues ."

eidos
"These outcomes indicate that USP10 inhibits CLP-induced apoptosis in renal tissues ."
USP10 affects CFTR-DeltaF508
| 2
USP10 activates CFTR-DeltaF508. 2 / 2
| 2

reach
"G3BP1-KD-induced CFTR-DeltaF508 aggregation is mediated by USP10 and p62."

reach
"USP10-KD and p62-KD augments cell toxicity of CFTR-DeltaF508."
USP10 affects CDKN2A
1 | 1
USP10 leads to the deubiquitination of CDKN2A. 2 / 2
1 | 1

"Oncogene-induced senescence mediated by c-Myc requires USP10 dependent deubiquitination and stabilization of p14ARF."

reach
"USP10, in turn, mediated the deubiquitination of p14ARF, preventing its proteasome dependent degradation."
USP10 affects BUB3
| 2
| 2

sparser
"These findings may be interpreted as BUB3 interacting with USP10 in the presence of MST1/2, which, in turn, increases the BUB3 protein level by interfering with its degradation."

sparser
"Specifically, we identified that, firstly, MST1/2 forms a PAH-specific interaction with cell cycle protein BUB3 and USP10, promoting the accumulation of BUB3 and consequent up-regulation of pro-proliferative pro-survival Akt and mTORC1 in PAH PAVSMC."
USP10 affects BAX
| 2
USP10 inhibits BAX.
| 1
USP10 inhibits BAX. 1 / 1
| 1

reach
"In the CLP group, overexpression of USP10 boosted the level of Bcl-2 and suppressed Bax and cleaved caspase-3 (Fig. 4B)."
USP10 decreases the amount of BAX.
| 1
USP10 decreases the amount of BAX. 1 / 1
| 1

reach
"Interestingly, downregulation of USP10 significantly decreased p53 stabilization and the expression of p53 target genes p21 and Bax after DNA damage (XREF_FIG)."
USP10 affects ANKRD28
2 |
2 |

No evidence text available

No evidence text available

reach
"For example, USP10 stimulates AR transcriptional activity in the presence of its synthetic agonist R1881 [67]."

reach
"Overexpression of USP10 enhanced the R1881 dependent response of all three tested minimal promoters, but had no significant effects on the unstimulated activity."
UBC affects USP10
2 |
2 |

No evidence text available

No evidence text available
TP53 affects G3BP1
| 2
TP53 binds USP10 and G3BP1. 2 / 2
| 2

sparser
"Disruption of the interaction of p53 with USP10 by G3BP1 interference leads to suppression of p53 deubiquitination."

sparser
"Disruption of the interaction of p53 with USP10 by G3BP1 interference leads to the suppression of p53 deubiquitination."
TOP2α affects RNF168
| 2
USP10 binds RNF168 and TOP2α. 2 / 2
| 2

sparser
"These novel functional interactions between RNF168, TOP2α and USP10 are likely to contribute to the maintenance of chromosomal integrity."

sparser
"Given that our mass spectrometry analysis identified USP10 as a possible RNF168-interacting partner ( xref ), we hypothesized that USP10 and RNF168 forms a complex with TOP2α to co-regulate its ubiquitylation level."
TLR2 affects USP10
| 2
TLR2 leads to the phosphorylation of USP10 on T674. 2 / 2
| 2

reach
"Collectively, these results indicated that BCR and TLR2 synergistically enhance Akt activity and promote USP10 T674 phosphorylation located within the NLS domain and, therefore, the nuclear import of USP10, consequently inhibiting the AID degradation in the nucleus.Pam 3 Csk 4 helped to enhance the antibody response to the SARS-CoV-2 RBD nanoparticle vaccine Pam 3 Csk 4 has been used as a vaccine adjuvant."

reach
"Collectively, these results indicated that BCR and TLR2 synergistically enhance Akt activity and promote USP10 T674 phosphorylation located within the NLS domain and, therefore, the nuclear import of USP10, consequently inhibiting the AID degradation in the nucleus.Pam3Csk4 has been used as a vaccine adjuvant.59 To explore the effect of Pam3Csk4 on the SARS-CoV-2 RBD particle vaccine immune response, USP10-BWT and USP10-BKO mice were immunized with adjuvants in combination with the SARS-CoV-2 RBD particle vaccine."
SQSTM1 affects G3BP1
| 2
| 2

sparser
"G3BP1 interacts with p62 and USP10 to inhibit protein aggregation."

sparser
"G3BP1 interacted with p62 and USP10 and inhibited p62/USP10-induced protein aggregation."
SOX21 affects USP10
| 1 1
| 1 1

reach
"Also, the binding of SOX21 and USP10 was also verified by RIP assay (Fig. 4G)."

sparser
"Also, the binding of SOX21 and USP10 was also verified by RIP assay (Fig.  xref G)."
SNX3 affects USP10
1 1 |
1 1 |

No evidence text available

No evidence text available
SNAI2 affects USP10
| 1 1
| 1 1

sparser
"USP10 interacts with Slug and mediates its degradation by the proteasome."

reach
"USP10 interacts with Slug and mediates its degradation by the proteasome."
SCNN1A affects USP10
1 1 |
1 1 |

No evidence text available

No evidence text available
RPL7A affects USP10
| 2
RPL7A binds USP10, PARP1, and NCL. 2 / 2
| 2

sparser
"Independent co-IP experiments using FLAG-tagged TARG1 from cell lysates in the presence of olaparib confirmed binding to ARTD1, NCL, RPL7A, and USP10 (Fig.  xref )."

sparser
"Independent co-IP experiments using FLAG-tagged TARG1 from cell lysates in the presence of olaparib confirmed binding to ARTD1, NCL, RPL7A, and USP10 (Fig. 1f)."
RNF168 affects TOP2α, and USP10
| 2
USP10 binds RNF168 and TOP2α. 2 / 2
| 2

sparser
"These novel functional interactions between RNF168, TOP2α and USP10 are likely to contribute to the maintenance of chromosomal integrity."

sparser
"Given that our mass spectrometry analysis identified USP10 as a possible RNF168-interacting partner ( xref ), we hypothesized that USP10 and RNF168 forms a complex with TOP2α to co-regulate its ubiquitylation level."
Particulate Matter increases the amount of USP10. 2 / 2
2 |

No evidence text available

No evidence text available
Pam3Csk4 affects USP10
| 2
Pam3Csk4 leads to the phosphorylation of USP10 on T674. 2 / 2
| 2

reach
"To further clarify that anti-μ antibody and Pam3Csk4 co-treatment promoted T674 phosphorylation of USP10, we first generated an antibody that specifically recognizes the phosphorylation site of USP10 by immunizing rabbits with the phosphorylated and sequence-homologous polypeptides (Supplementary Fig. 4a)."

reach
"Western blot analysis of Ramos B cells and mouse spleen B cells showed that the co-stimulation of anti-μ antibody and Pam3Csk4 promoted USP10 T674 phosphorylation, but not when AKT inhibitor was used (Fig. 6i, j)."
PPP6R1 affects USP10
2 |
2 |

No evidence text available

No evidence text available
PGK1 affects USP10
| 2
| 2

reach
"Together, our study demonstrated for the first time that KIF15 enhances the glycolytic capacity of PC through the recruitment of USP10 and PGK1, and that the KIF15/USP10/PGK1 axis may serve as an effective therapeutic agent for PC."

reach
"The MS and Co-IP assay indicated that KIF15 recruited and enhanced the binding between PGK1 and USP10."
PARP1 affects USP10
| 2
RPL7A binds USP10, PARP1, and NCL. 2 / 2
| 2

sparser
"Independent co-IP experiments using FLAG-tagged TARG1 from cell lysates in the presence of olaparib confirmed binding to ARTD1, NCL, RPL7A, and USP10 (Fig.  xref )."

sparser
"Independent co-IP experiments using FLAG-tagged TARG1 from cell lysates in the presence of olaparib confirmed binding to ARTD1, NCL, RPL7A, and USP10 (Fig. 1f)."
PABPC1 affects CAPRIN1
| 2
| 2

sparser
"Finally, acetylation of Lysine-376 (K376) in the RRM domain, impairs the RNA binding function of G3BP1 as well as its interaction with PABP1, USP10 or Caprin1[ xref ]."

sparser
"As previously discussed, acetylation of K376 of G3BP1 by the CBP/P300 acetylase is a key modification impeding SGs assembly by impairing its RNA binding function, as well as its interaction with PABP1, USP10 or Caprin1[ xref ]."
NUTF2 affects USP10
| 2
| 2

reach
"USP10 's interaction with G3BP1 involves an FGDF motif within USP10 that binds a pocket within the N-terminal NTF2 like domain of G3BP1, and this binding inhibits SG condensation in response to some stresses [XREF_BIBR, XREF_BIBR]."

reach
"We wondered whether this interaction displaces USP10, a cellular binding partner of G3BP that also binds to the NTF2 like domain [XREF_BIBR, XREF_BIBR]."
NCL affects USP10
| 2
RPL7A binds USP10, PARP1, and NCL. 2 / 2
| 2

sparser
"Independent co-IP experiments using FLAG-tagged TARG1 from cell lysates in the presence of olaparib confirmed binding to ARTD1, NCL, RPL7A, and USP10 (Fig.  xref )."

sparser
"Independent co-IP experiments using FLAG-tagged TARG1 from cell lysates in the presence of olaparib confirmed binding to ARTD1, NCL, RPL7A, and USP10 (Fig. 1f)."
MYCBP2 affects USP10
| 2
MYCBP2 leads to the phosphorylation of USP10 on T674. 2 / 2
| 2

reach
"To further clarify that anti-μ antibody and Pam 3 Csk 4 co-treatment promoted T674 phosphorylation of USP10, we first generated an antibody that specifically recognizes the phosphorylation site of USP10 by ."

reach
"Western blot analysis of Ramos B cells and mouse spleen B cells showed that the co-stimulation of anti-μ antibody and Pam 3 Csk 4 promoted USP10 T674 phosphorylation, but not when AKT inhibitor was used (Fig. 6i, j) ."
MST1/2 affects USP10
| 2
MST1/2 activates USP10. 2 / 2
| 2

reach
"In PAH PAVSMC, MST1/2 acted via forming a disease-specific interaction with BUB3 and supported extracellular matrix- and USP10-dependent BUB3 accumulation, up-regulation of Akt-mTORC1, cell proliferation, and survival."

reach
"In PAH pulmonary arterial vascular smooth muscle cell, MST1/2 acted via forming a disease-specific interaction with BUB3 and supported ECM (extracellular matrix)- and USP10-dependent BUB3 accumulation, upregulation of Akt-mTORC1, cell proliferation, and survival."
MDM2 affects USP10
1 | 1
1 | 1

No evidence text available

sparser
"Notably, USP10 does not bind to MDM2 [ xref ]."
KLHDC3 affects USP10
2 |
2 |

No evidence text available

No evidence text available
Histone affects USP10
| 2
Histone activates USP10. 2 / 2
| 2

reach
"To test whether USP10 targets histones as substrates, we co-expressed Flag tagged USP10 (Flag-USP10) with tagged H2A.Z or H2A in 293T cells and measured the levels of ubiquitylated histones by Western blot."

reach
"Supporting our finding that USP10 targets histones, cells with stable knockdown of USP10 consistently have higher levels of H2A.Zub1 and H2Aub1, whereas the levels of H2Bub1 remain unchanged (XREF_FIG D)."
GST affects USP10
| 2
| 2

sparser
"As shown in Fig. xref , bacterially-purified GST-USP10 and His-HDAC6 were able to interact with each other under cell-free conditions via a GST pull-down assay, suggesting a direct interaction between USP10 and HDAC6."

sparser
"To determine whether USP10 binds to HDAC6 directly or via other proteins, we generated and purified recombinant GST-USP10 and His-HDAC6 in E. coli ."
GASAL1 affects USP10
| 1 1
GASAL1 activates USP10. 1 / 1
| 1

reach
"GASAL1 could up-regulate USP10 via competitively binding to miR-193b-5p."
GASAL1 activates USP10. 1 / 1
| 1

eidos
"GASAL1 could up-regulate USP10 via competitively binding to miR-193b-5p ."
G3BP1 affects TP53, and USP10
| 2
TP53 binds USP10 and G3BP1. 2 / 2
| 2

sparser
"Disruption of the interaction of p53 with USP10 by G3BP1 interference leads to suppression of p53 deubiquitination."

sparser
"Disruption of the interaction of p53 with USP10 by G3BP1 interference leads to the suppression of p53 deubiquitination."
G3BP1 affects SQSTM1, and USP10
| 2
| 2

sparser
"G3BP1 interacts with p62 and USP10 to inhibit protein aggregation."

sparser
"G3BP1 interacted with p62 and USP10 and inhibited p62/USP10-induced protein aggregation."
FlaG affects USP10
| 2
| 2

sparser
"We observed that addition of His6-USP10, but not His6-USP13, reduced the amount of HA-Ub-conjugated FLAG-LC3B ( xref , C and D ), consistent with USP10 specifically catalyzing the deubiquitination of LC3B. Finally, we found that GFP-tagged LC3B (GFP-LC3B) co-immunoprecipitated with FLAG-USP10 but not FLAG-USP13 ( xref E )."

sparser
"Cells were transfected with FLAG-USP10 (green) and we found that FLAG-tagged USP10 appeared to be co-localized with endogenous CD36 ( xref )."
EGFP affects USP10
| 2
| 2

sparser
"Following induction of EGFPUSP10(1-40) or control EGFP protein expression, we treated these cells with UV or sodium arsenite, and analysed GCN2 and eIF2α phosphorylation using western blotting ( xref E) and SG formation using immunofluorescence staining ( xref F)."

sparser
"By contrast, SG formation in response to both treatments was blocked in EGFP-USP10(1-40)-expressing cells ( xref F)."
DENV-2 RNA affects USP10
| 2
USP10 binds DENV-2 RNA. 2 / 2
| 2

reach
"In addition, a quantitative mass spectrometry study revealed that DENV-2 RNA interacts with the SG components G3BP-1/2, Caprin1, and USP10."

reach
"In addition, a quantitative mass spectrometry study revealed that DENV-2 RNA interacts with the SG components G3BP-1/2, Caprin1, and USP10 (Ward et al., 2011)."
DCN affects USP10
| 2
DCN activates USP10. 2 / 2
| 2

reach
"As ubiqutin signaling serves as an early harbinger of autophagy, decorin might augment the USP10 activity (or generally modulate ubiquitin signaling in favor of autophagy) for increased Beclin 1 or Peg3 stability."

reach
"As ubiqutin signaling serves as an early harbinger of autophagy, decorin might augment the USP10 activity (or generally modulate ubiquitin signaling in favor of autophagy) for increased Beclin 1 or Peg3 stability."
CAPRIN1 affects PABPC1, and USP10
| 2
| 2

sparser
"Finally, acetylation of Lysine-376 (K376) in the RRM domain, impairs the RNA binding function of G3BP1 as well as its interaction with PABP1, USP10 or Caprin1[ xref ]."

sparser
"As previously discussed, acetylation of K376 of G3BP1 by the CBP/P300 acetylase is a key modification impeding SGs assembly by impairing its RNA binding function, as well as its interaction with PABP1, USP10 or Caprin1[ xref ]."
BUB3 affects USP10
| 2
| 2

sparser
"These findings may be interpreted as BUB3 interacting with USP10 in the presence of MST1/2, which, in turn, increases the BUB3 protein level by interfering with its degradation."

sparser
"Specifically, we identified that, firstly, MST1/2 forms a PAH-specific interaction with cell cycle protein BUB3 and USP10, promoting the accumulation of BUB3 and consequent up-regulation of pro-proliferative pro-survival Akt and mTORC1 in PAH PAVSMC."
BECN1 affects USP13
| 2
| 2

sparser
"To determine the mechanism by which Vps34 complexes regulate the stability of USP13 and USP10, we examined the possibility that Beclin1 may interact with USP10 and USP13."

sparser
"Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10."
ANLN affects USP10
| 2
| 2

sparser
"Furthermore, the findings of the study showed that the deubiquitinating enzyme USP10 interacts with ANLN and positively regulates ANLN protein levels."

sparser
"Overall, targeting the USP10-ANLN axis can effectively inhibit ESCC cell-cycle progression."
ANKRD28 affects USP10
2 |
2 |

No evidence text available

No evidence text available
Virulence Cif found patients CF chronic obstructive pulmonary disease affects USP10
| 1
Virulence Cif found patients CF chronic obstructive pulmonary disease inhibits USP10. 1 / 1
| 1

eidos
"Pseudomonas aeruginosa virulence factor Cif , found in patients with CF and chronic obstructive pulmonary disease ( COPD ) , suppresses USP10 activity and promotes CFTR ubiquitylation and lysosomal degradation ( Figure 3 ) [ 56 ] ."
Stress granule proteins affects USP10
| 1
Stress granule proteins inhibits USP10. 1 / 1
| 1

reach
"Depletion of stress granule proteins decreased the abundance of HCV proteins and RNAs (XREF_FIG), and G3BP1 and USP10 localized with HCV core protein at lipid droplets."
Spautin -1 affects USP10
| 1
Spautin -1 inhibits USP10. 1 / 1
| 1

reach
"Moreover, suppressors such as SAR405 inhibit Vp34 and lead to the alteration of lysosomal function [100], while spautin -1 inhibits USP10 and USP13 peptidases (ubiquitin-specific peptidases) [102]."
| PMC
1 |
Sodium fluoride increases the amount of USP10. 1 / 1
1 |

No evidence text available
Small‐molecule inhibitor Spautin‐1 affects USP10
| 1
Small‐molecule inhibitor Spautin‐1 inhibits USP10. 1 / 1
| 1

reach
"To further validate the regulatory effect of USP10 on Smad4, we introduced a small‐molecule inhibitor Spautin‐1 (Liu et al., 2011), which can specifically suppress deubiquitinase activity of USP10."
SfRNAs affects USP10
| 1
SfRNAs inhibits USP10. 1 / 1
| 1

eidos
"G3BP1 , G3BP2 , Caprin1 , and USP10 are sequestered by sfRNAs , which leads to inhibition of ISG mRNA translation and thus , attenuation of the antiviral response [ 235 ] ."
1 |
Schizandrin B increases the amount of USP10. 1 / 1
1 |

No evidence text available
1 |
Pentachlorophenol increases the amount of USP10. 1 / 1
1 |

No evidence text available
MiR-103 affects USP10
| 1
MiR-103 decreases the amount of USP10. 1 / 1
| 1

reach
"In addition, by using qRT-PCR assay and western blotting analysis, we found that miR-103 down-regulated the expression of USP10 in PaCa tissues and cell lines."
Imaging-based screening affects USP10
| 1
Imaging-based screening inhibits USP10. 1 / 1
| 1

eidos
"USP10 / 13 Inhibitors Through an imaging-based screening and subsequent structural modification , a potent autophagy inhibitor spautin-1 ( Figure 19 ) was identified , which was demonstrated to inhibit USP10 and USP13 and thus promote the degradation of Vps34 PI3 kinase complexes [ 152 ] ."
1 |
Hsa-miR-652-3p decreases the amount of USP10. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-455-3p decreases the amount of USP10. 1 / 1
1 |

No evidence text available
1 |
Geldanamycin increases the amount of USP10. 1 / 1
1 |

No evidence text available
| 1

reach
"Current studies are underway to elucidate the mechanism by which the epoxide hydrolase activity of Cif promotes the inactivation of USP10, via enhancement of G3BP1 interaction, leading to the degradation of CFTR."
Doxycycline affects USP10
| 1
| 1

reach
"XREF_FIG, doxycycline induction of USP10 shRNA (Dox+), but not the vehicle (Dox-), led to a significant reduction of USP10 in H23 cells."
Dicrotophos affects USP10
1 |
Dicrotophos increases the amount of USP10. 1 / 1
1 |

No evidence text available
Choline affects USP10
1 |
Choline methylates USP10. 1 / 1
1 |

No evidence text available
Autophagy affects USP10
| 1
| 1

eidos
"In this study , we identified spautin-1 , an autophagy inhibitor that suppresses ubiquitin-specific peptidase 10 ( USP10 ) and USP13 , as a novel UPR inhibitor under glucose starvation conditions ."
Ato affects USP10
| 1
Ato inhibits USP10. 1 / 1
| 1

reach
"Previous reports had suggested that ATO could degrade FLT3 protein by downregulating USP10 to inhibit FLT3-ITD positive cells, but ATO at low concentrations (0.5 microM) failed to downregulate USP10 and could not induce degradation of total FLT3 and modulation of 160KD or 130KD FLT3 (Additional file 1 : Figure S1A, B)."
AhpC affects USP10
| 1
AhpC leads to the dephosphorylation of USP10. 1 / 1
| 1

reach
"Mutation of both T42 and S337 (TS/AA) also abolished USP10 phosphorylation by ATM (XREF_FIG)."
ZC3H18 affects USP10
1 |
1 |

No evidence text available
ZC3H12A affects NPPA
| 1
| 1

sparser
"TRAF family member-associated NF- κ B activator (TANK) interacts with both MCPIP1 and USP10, which leads to decrease in TRAF6 ubiquitination and the termination of the NF- κ B activation in response to TLR activation [ xref ]."
VNS affects USP10
| 1
VNS increases the amount of USP10. 1 / 1
| 1

reach
"VNS enhanced the expression of USP10 following tMCAO."
USP15 affects USP10
1 |
1 |

No evidence text available
USP10 affects ubiquitination reduction
| 1
USP10 inhibits ubiquitination reduction. 1 / 1
| 1

eidos
"USP10 decreases the ubiquitination of CFTR , whereas the knockdown of USP10 promotes the ubiquitination and reduction of CFTR at the cell surface [ 85 ] ."
| PMC
USP10 affects ubiquitination LC3B
| 1
USP10 inhibits ubiquitination LC3B. 1 / 1
| 1

eidos
"These results thus indicated that USP10 reduces the ubiquitination of not only LC3B , but also LC3A and LC3C ."
USP10 affects stopping cell cycle cancer cell growth
| 1
USP10 inhibits stopping cell cycle cancer cell growth. 1 / 1
| 1

eidos
"Additionally , USP10 blocks tumor formation via p53 and SIRT6-mediated degradation of c-Myc , thereby stopping cell cycle progression and cancer cell growth [ 53 ] ."
| PMC
USP10 affects sdRNA
| 1
USP10 activates sdRNA. 1 / 1
| 1

reach
"In this study, we tested whether knockdown of USP10 with a USP10 targeting sdRNA (termed US09) will reduce scarring after wounding a rabbit cornea invivo."
USP10 affects same results
| 1
USP10 inhibits same results. 1 / 1
| 1

eidos
"As shown in Figure 2C , 2D , knockdown of USP10 induced the same results using oil red O assay ."
USP10 affects removal NEMO-attached M1-linked polyubiquitin chains
| 1
USP10 activates removal NEMO-attached M1-linked polyubiquitin chains. 1 / 1
| 1

eidos
"] Of note , USP10 also interacts with NEMO via MCPIP1 and leads to the removal of NEMO-attached M1-linked polyubiquitin chains , thus inhibiting the genotoxic NF-kappaB signaling cascade ."

eidos
"In the case of DNA damage , the phosphorylation of USP10 at Thr42 and Ser337 mediated by ATM is essential for its translocation to the nucleus , where USP10 induces the deubiquitination of p53 and makes p53 work as a tumor suppressor ( Fig. 2c ) .122 In addition to ubiquitination , p53 can also undergo other ubiquitination-like modifications , such as neddylation and SUMOylation ."
USP10 affects nppb
| 1
USP10 decreases the amount of nppb. 1 / 1
| 1

reach
"Compared with AdGFP transfection, AdUSP10 notably alleviated Ang II induced cardiomyocyte enlargement, accompanied by significant decreases in the expression of the fetal genes Anp, Bnp, and Myh7; in contrast, USP10 silencing significantly exacerbated myocyte hypertrophy and upregulated the expression of Anp, Bnp, and Myh7 compared with that in primary cells infected with AdshRNA, suggesting that USP10 can directly mitigate the hypertrophic growth of isolated myocytes induced by Ang II."
USP10 affects lipid uptake
| 1
USP10 activates lipid uptake. 1 / 1
| 1

eidos
"We have clarified that USP10 inhibition suppresses lipid uptake ."
USP10 affects limonin
| 1
USP10 activates limonin. 1 / 1
| 1

reach
"Furthermore, limonin inhibited the degradation of SIRT6 by activating ubiquitin-specific peptidase (Cuspidi et al.)-10, while USP10 siRNA abrogated the beneficial effects of limonin."
USP10 affects inflammatory cytokine induction
| 1
USP10 inhibits inflammatory cytokine induction. 1 / 1
| 1

eidos
"A previous study demonstrated that USP10 inhibits NF-kappaB activation and inflammatory cytokine induction ( 22 ) ."
USP10 affects inflammation response
| 1
USP10 inhibits inflammation response. 1 / 1
| 1

eidos
"USP10 knockdown enhances the inflammation response during hepatic I / R. ( A , B ) The mRNA level of pro-inflammatory factors ( TNF-alpha , IL6 , IL1beta , and Ccl2 ) at 6 h ( A ) and 24 h ( B ) after I / R injury in USP10-HZ and WT mice and in sham controls ( n = 4 per group ) ."
USP10 affects imidazolide
| 1
| 1

reach
"To determine whether inhibition of USP10 enhances the efficacy of IM, we examined the effect of Spautin-1 or various concentrations of IM alone or in combination on the cell viability of CML cells and primary monocytes from patients."
USP10 affects hepatic R process
| 1
USP10 inhibits hepatic R process. 1 / 1
| 1

eidos
"USP10 inhibited TAK1-JNK / p38 signalling in hepatic I / R process ."
USP10 affects foam cell cells
| 1
USP10 activates foam cell cells. 1 / 1
| 1

eidos
"Oil red O assay showed that stained cell number was decreased in the treatment of USP10 inhibitor , suggesting that inhibition of USP10 blocks the foam cell formation in THP1 and RAW264.7 cells ( Figure 2A , 2B ) ."
USP10 affects extracellular core protein
| 1
USP10 activates extracellular core protein. 1 / 1
| 1

reach
"Curiously, depletion of USP10 increased the abundance of extracellular core protein (XREF_FIG)."
USP10 affects dopamine-induced reactive oxygen species-dependent apoptosis neuronal cells
| 1
USP10 inhibits dopamine-induced reactive oxygen species-dependent apoptosis neuronal cells. 1 / 1
| 1

eidos
"USP10 inhibits the dopamine-induced reactive oxygen species-dependent apoptosis of neuronal cells by stimulating the antioxidant Nrf2 activity ."
USP10 affects dopamine-induced Nrf2 translation
| 1
USP10 activates dopamine-induced Nrf2 translation. 1 / 1
| 1

eidos
"In addition , USP10 augmented dopamine-induced Nrf2 translation ."
USP10 affects deubiquitination human NHE3 regulating
| 1
USP10 activates deubiquitination human NHE3 regulating. 1 / 1
| 1

eidos
"Ubiquitin-specific peptidase 7 ( USP7 ) and USP10 mediate deubiquitination of human NHE3 regulating its expression and activity ."

reach
"Usp10 inhibition attenuated H 2 O 2 -induced senescence in MLO-Y4 cells, as indicated by p53 and p21 quantification, a senescence associated beta-galactosidase (SA-beta-gal) assay, and p53 localization visualization with a confocal microscope."
USP10 affects cell growth invasion lung cancer
| 1
USP10 inhibits cell growth invasion lung cancer. 1 / 1
| 1

eidos
"Moreover , USP10 inhibited cell growth and invasion in lung cancer [ 78 ] , and was an independent factor for the prognosis of gastric carcinoma [ 79 ] ."
USP10 affects c-KIT
| 1
USP10 activates c-KIT. 1 / 1
| 1

reach
"Thus, USP10 might deubiquitinate c-CBL substrate (s) and positively regulate c-KIT signaling."
USP10 affects affecting unknown
| 1
USP10 inhibits affecting unknown. 1 / 1
| 1

eidos
"We first hypothesize that in a wild-type p53 background , depletion of USP10 leads to the reduction of HDAC6 as well as affecting an unknown factor , which counteracts the effect of HDAC6 reduction ."
USP10 affects activation NRF2 HO-1 pathway SIRT6
| 1
USP10 activates activation NRF2 HO-1 pathway SIRT6. 1 / 1
| 1

eidos
"USP10 promotes activation of the NRF2 / HO-1 pathway through SIRT6 and attenuates LPS-induced renal tubular epithelial cell injury ."
USP10 affects abundance YAP TAZ
| 1
USP10 activates abundance YAP TAZ. 1 / 1
| 1

eidos
"The abundance of YAP and TAZ was potently increased by USP10 , which removed their poly-ubiquitin chains directly in HCC ( Zhu et al ., 2020 ) ."
USP10 affects ZC3H18
1 |
1 |

No evidence text available
USP10 affects XRCC1
| 1
USP10 inhibits XRCC1. 1 / 1
| 1

reach
"The reconstitution of USP10 inhibited the colony formation and cell proliferation of the CAKI-1 and CAKI-2 RCC cell lines."
USP10 affects USP15
1 |
1 |

No evidence text available
USP10 affects USP10 inhibitors
| 1
USP10 inhibits USP10 inhibitors. 1 / 1
| 1

reach
"Looking forward, cancer-type-specific USP10 animal models will accelerate and improve the development of specific USP10 inhibitors."
USP10 affects TNS
| 1
| 1

sparser
"The interaction between USP10 and Phosphatase And Tensin Homolog (PTEN) was examined by co-immunoprecipitation."
USP10 affects TARDBP
1 |
1 |

No evidence text available
USP10 affects TAK1-JNK p38 Activation Hepatic R Injury MAPKs play mediating inflammatory response cell death
| 1
USP10 inhibits TAK1-JNK p38 Activation Hepatic R Injury MAPKs play mediating inflammatory response cell death. 1 / 1
| 1

eidos
"USP10 Inhibits TAK1-JNK / p38 Activation During Hepatic I / R Injury MAPKs play an important role in mediating the inflammatory response and cell death ."
USP10 affects Recycling
| 1
| 1

eidos
"Endosome-localized DUB , Ubiquitin Specific Protease 10 ( USP10 ) , suppresses ubiquitylation of WT-CFTR in early endosomes and promotes the recycling of CFTR to the PM [ 55 ] ."
USP10 affects RPS6
| 1
USP10 decreases the amount of RPS6. 1 / 1
| 1

reach
"As anticipated, overexpressing USP10 markedly inhibited the expression of P-AKT and P-S6, and increases P-AMPKalpha expression in vivo."
USP10 affects RNF123
| 1
| 1

sparser
"As shown in xref , the association of RNF123 and USP10 with the ribosome complex was increased at early times after UV irradiation."
USP10 affects Quercetin.USP10
| 1
USP10 activates Quercetin.USP10. 1 / 1
| 1

reach
"Therefore, USP10 may be also a target of Quercetin.USP10 could prevent T-bet degradation and enhanced its protein level."
USP10 affects Parkinson
| 1
USP10 activates Parkinson. 1 / 1
| 1

eidos
"Indeed , G3BP1 was recently described to promote proteasomal function and inhibit accumulation of ubiquitinated protein aggregates associated with Parkinson 's ( alpha-synuclein ) and Cystic Fibrosis ( CFTR-DeltaF508 ) , which were induced by p62 and USP10 ."
USP10 affects PI3K
| 1
USP10 inhibits PI3K. 1 / 1
| 1

reach
"While A20 inhibits PtdIns3P signaling by removing the TRAF6 dependent Lys63 linked chains from Beclin 1, the enzymes USP10 and USP13 prevent PI3K and III complex components from their degradation and, therefore, support autophagy."
USP10 affects P88924
1 |
1 |

No evidence text available
USP10 affects NSCLC
| 1
USP10 activates NSCLC. 1 / 1
| 1

reach
"HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway."
USP10 affects NPPA
| 1
| 1

sparser
"TRAF family member-associated NF- κ B activator (TANK) interacts with both MCPIP1 and USP10, which leads to decrease in TRAF6 ubiquitination and the termination of the NF- κ B activation in response to TLR activation [ xref ]."
USP10 affects NASH
| 1
USP10 activates NASH. 1 / 1
| 1

reach
"USP10 could promote autophagy-related pathways and molecules and increase the synthesis of autophagosomes in NASH, improving steatosis, inflammation, and fibrosis."
USP10 affects K63-linked polyubiquitination mediated TRIM25 non-small cell lung cancer
| 1
USP10 inhibits K63-linked polyubiquitination mediated TRIM25 non-small cell lung cancer. 1 / 1
| 1

eidos
"We found using co-immununoprecipitation / immunoblotting that USP10 interacted with PTEN and reduced the K63-linked polyubiquitination of PTEN mediated by TRIM25 in non-small cell lung cancer ( NSCLC ) cells ."
USP10 affects ITD [42]
| 1
USP10 activates ITD [42]. 1 / 1
| 1

reach
"It was recently reported that inhibition of USP10 also induces degradation of FLT3/ITD [42]."
USP10 affects IGF2BP1
| 1
| 1

reach
"USP10 can bind to, deubiquitinate, and stabilize IGF2BP1, resulting in its higher expression level in breast cancer."
USP10 affects IBTK
1 |
1 |

No evidence text available
USP10 affects HUWE1
| 1
USP10 inhibits HUWE1. 1 / 1
| 1

reach
"We found that Huwe1 activity on TBP is antagonized by the deubiquitinase USP10, which protects TBP from degradation."
USP10 affects HDAC_III
| 1

reach
"USP10 physically binds to sirtuin 6 and sirtuin 6 mediates the effect of USP10 on the regulation of cardiac hypertrophy."
USP10 affects H2BC10
1 |
USP10 deubiquitinates H2BC10. 1 / 1
1 |

"The deubiquitinase (DUB) Ubp10 is thought to promote heterochromatic silencing by maintaining low H2B-Ub at sub-telomeres"
USP10 affects GR
| 1
USP10 inhibits GR. 1 / 1
| 1

reach
"In particular, G3BP1, G3BP2, and Caprin1, promoters of stress granule assembly, enhanced GR-mediated toxicity while USP10, an inhibitor of stress granule assembly, suppressed GR toxicity (Lee et al., 2016)."
| 1
| 1

reach
"Especially for inflammation, Li et al. demonstrated that USP10 can elevate proinflammatory factors in endometriosis (EM) and that Cai’s Neiyi Prescription (CNYP) can reduce EM-induced inflammation by inhibiting the mRNA and protein expression of USP10 [12]."
USP10 affects DDX21
| 1
USP10 activates DDX21. 1 / 1
| 1

reach
"LINC00240 in the 6p22.1 risk locus promotes gastric cancer progression through USP10-mediated DDX21 stabilization."
USP10 affects Cyclin
| 1
USP10 activates Cyclin. 1 / 1
| 1

reach
"Inhibition of USP10 induces myeloma cell apoptosis by promoting cyclin D3 degradation."

reach
"Deubiquitinating enzyme USP10 promotes hepatocellular carcinoma metastasis through deubiquitinating and stabilizing Smad4 protein."
USP10 affects CCND3/CDK4/6
| 1
USP10 activates CCND3/CDK4/6. 1 / 1
| 1

reach
"By stabilizing CCND3, USP10 activated the CCND3/CDK4/6 signaling pathway, phosphorylated Rb, and upregulated CDK4, CDK6 and E2F-1 in OPM2 and KMS11 cells."
USP10 affects Bcr-Abl
| 1
Modified USP10 leads to the phosphorylation of Bcr-Abl. 1 / 1
| 1

reach
"The result showed that loss of USP10 significantly decreased the expression of SKP2, as well as the phosphorylation and downstream signals of Bcr-Abl, suggesting that USP10 is required for the expression of SKP2 and the phosphorylation of Bcr-Abl in CML cells."
USP10 affects AngII-induced Akt
| 1
USP10 activates AngII-induced Akt. 1 / 1
| 1

eidos
"USP10 Directly Regulates Sirt6 During Cardiac Hypertrophy To delineate the mechanisms by which USP10 expression modulates AngII-induced Akt activation , we investigated whether USP10 affect the stability of Sirt6 , which has been shown to block insulin-like growth factor-Akt signaling and the development of CH ."
USP10 affects ARF
| 1
USP10 deubiquitinates ARF. 1 / 1
| 1

reach
"USP10 deubiquitinates ARF by interacting with the N-terminus of ARF, thereby stabilizing the ARF protein by blocking the proteasome-mediated ARF degradation."
TNS affects USP10
| 1
| 1

sparser
"The interaction between USP10 and Phosphatase And Tensin Homolog (PTEN) was examined by co-immunoprecipitation."
TARDBP affects USP10
1 |
1 |

No evidence text available
Spain-1 affects USP10
| 1
Spain-1 inhibits USP10. 1 / 1
| 1

reach
"In addition, Spain-1, an inhibitor of USP10, inhibits melanoma growth and improves the anticancer effect of cisplatin by inhibiting USP10 activity [87,88]."
Sesame Oil affects USP10
1 |
Sesame Oil increases the amount of USP10. 1 / 1
1 |

No evidence text available
SNCA affects USP10
| 1
| 1

sparser
"In cultured cells, USP10 promoted α-synuclein-induced aggresome formation ( xref A) and USP10 was often detected at the periphery of α-synuclein-positive aggresomes ( xref C and xref C)."
RNF123 affects USP10
| 1
| 1

sparser
"As shown in xref , the association of RNF123 and USP10 with the ribosome complex was increased at early times after UV irradiation."
P88924 affects USP10
1 |
1 |

No evidence text available
Niclosamide affects USP10
1 |
Niclosamide decreases the amount of USP10. 1 / 1
1 |

No evidence text available
NPPA affects ZC3H12A
| 1
| 1

sparser
"TRAF family member-associated NF- κ B activator (TANK) interacts with both MCPIP1 and USP10, which leads to decrease in TRAF6 ubiquitination and the termination of the NF- κ B activation in response to TLR activation [ xref ]."
NANOG affects USP10, USP16, USP3, USP37, USP44, and USP7
| 1
| 1

sparser
"USP25, USP44, USP49, and USP7 bind to the Sox2 promoter, while USP10, USP16, USP3, USP37, USP44, and USP7 bind to the Nanog promoter."

reach
"MG-132 treatment reduced the amount of USP10, and the reduction is attenuated by cotreatment with BafA1 (XREF_FIG B), indicating that USP10 is degraded by autophagy."
MITF affects USP10
| 1
MITF activates USP10. 1 / 1
| 1

reach
"In conclusion, FSTL1 treatment ameliorated cardiac dysfunction in MI with co-existent T2DM, possibly through inhibition of myocardial fibrosis and apoptosis by upregulating USP10/Notch1 signaling."
1 |
1 |

No evidence text available
Ischemia affects USP10
| 1
Ischemia increases the amount of USP10. 1 / 1
| 1

reach
"Low expression of USP10 induced by cerebral ischemia/reperfusion is associated with brain injury, and increased USP10 expression has a protective effect against cerebral ischemia injury by inhibiting inflammation and apoptosis through blocking TAK1 signal transduction [8]."
IGF2BP1 affects USP10
| 1
| 1

reach
"USP10 can bind to, deubiquitinate, and stabilize IGF2BP1, resulting in its higher expression level in breast cancer."
IBTK affects USP10
1 |
1 |

No evidence text available
HDAC_III affects USP10
| 1

reach
"USP10 physically binds to sirtuin 6 and sirtuin 6 mediates the effect of USP10 on the regulation of cardiac hypertrophy."
BLLF1 affects USP10
| 1
BLLF1 inhibits USP10. 1 / 1
| 1

reach
"One of the first evidences of the participation of RNA modifications in the dynamics of SGs was the observation that m A disrupts RNA binding by G3BP1/2, ubiquitin-specific peptidase 10 (USP10), cell cycle-associated protein 1 (CAPRIN1), and RNA-binding motif protein 42 (RBM42), all proteins of SGs (Arguello et al., 2017; Edupuganti et al., 2017)."