IndraLab

Statements

Ubiquitin affects UCHL3
| 1 7 39
Ubiquitin binds UCHL3.
| 1 35
Ubiquitin binds UCHL3. 10 / 35
| 1 30
sparser
"Next we quantified the change in chemical shift induced upon binding of ubiquitin to UCH-L3."
10518943
sparser
"Having defined the surface of ubiquitin that interacts with UCH-L3, we next sought to identify candidate residues on UCH-L3 that interact with ubiquitin."
10518943
sparser
"The high preference at the P3 position and relatively low specificity in the P4 position can be explained by comparison with the published crystal structure of UCH-L3 bound with ubiquitin inhibitor (Fig.3) ."
18601651
sparser
"The high preference at the P3 position and relatively low specificity in the P4 position can be explained by comparison with the published crystal structure of UCH-L3 bound with ubiquitin inhibitor ( xref )."
18601651
sparser
"From a crystal structure of a complex between UCHL3 and synthetic K27 Ub 2 , we unexpectedly discover that free K27 Ub 2 and K27 Ub 2 -conjugated substrates are natural inhibitors of UCHL3."
33238157
sparser
"In contrast, when UCH-L3 is bound to an ubiquitin-based inhibitor, ubiquitin vinyl methyl ester, the crossover loop becomes stabilized in an α-helix followed by an S shaped loop forming contacts with the C terminus of ubiquitin ( xref )."
19489724
sparser
"To determine if these electrostatic interactions contribute significantly to the stability of the ubiquitin·UCH-L3 complex, we mutated a number of basic residues on the surface of ubiquitin and measur[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10518943
sparser
"Since ubiquitin binding orders a significant portion of the protein, the UCH domain of BAP1 was modeled after Uch-L3 bound to ubiquitin (PDB: 1xd3)."
22683710
sparser
"Information for structures was from the protein data bank (PDB): ubiquitin from UCH-L3-Ub complex (PDB code: 1XD3) and ISG15 (PDB code: 1Z2M)."
20153823
sparser
"Model showing binding of ubiquitin aldehyde to UCH-L3 using PDB accession 1XD3: blue -the size dominant exosite that binds epitopes on the ubiquitin surface, red -the smaller linear epitope corresponding to the C-terminal tetrapeptide (P2-P4) that binds the cleavage site cleft of the enzymes, purple -Arg at P5."
18601651
Ubiquitin binds UCHL1 and UCHL3. 3 / 3
| 3
sparser
"T218, R227, F228, and L230 residues of BAP1N were selected based on ubiquitin interaction with the corresponding residues of UCHL1 and UCHL3."
28935764
sparser
"In contrast with BAP1, the thermodynamics of ubiquitin binding to UCHL1 and UCHL3 is dominated by enthalpy factors and hydrogen bonding and ionic interactions that stabilize the complex."
28935764
sparser
"Unlike UCH-L1, UCH-L3 can interact with Lys48-linked Ub dimers (di-Ub) to protect it from degradation and meanwhile to inhibit its hydrolase activity."
20302916
Ubiquitin binds UCHL3 and LMO7. 1 / 1
| 1
sparser
"We suggest that the increase in severity may reflect an interaction between Uchl3 and Lmo7 in the ubiquitin-mediated protein degradation pathway."
12761045
Ubiquitin binds UCHL3 and NEDD8. 1 / 1
| 1
sparser
"In vitro GST pull-down assay demonstrated that UCH-L3 bound to both NEDD8 and ubiquitin."
9790970
Ubiquitin inhibits UCHL3.
| 1 5 4
Ubiquitin inhibits UCHL3. 10 / 11
| 1 5 4
reach
"As an additional control experiment, we measured a Ki of 0.27 μM for the inhibition of UCH-L3 by ubiquitin with the substrate ubiquitin-AMC."
16905103
sparser
"As an additional control experiment, we measured a K of 0.27 μM for the inhibition of UCH-L3 by ubiquitin with the substrate ubiquitin-AMC."
16905103
sparser
"As an additional control experiment, we measured a Ki of 0.27 μM for the inhibition of UCH-L3 by ubiquitin with the substrate ubiquitin-AMC."
16905103
reach
"The present data demonstrated that ubiquitin aldehyde can inhibit the activity of UCHL3."
27780264
eidos
"As an additional control experiment , we measured a K i of 0.27 mM for the inhibition of UCH-L3 by ubiquitin with the substrate ubiquitin-AMC ."
16905103
reach
"As an additional control experiment, we measured a K i of 0.27 mM for the inhibition of UCH-L3 by ubiquitin with the substrate ubiquitin-AMC."
16905103
reach
"As an additional control experiment, we measured a K i of 0.27 muM for the inhibition of UCH-L3 by ubiquitin with the substrate ubiquitin-AMC."
16905103
sparser
"Using our Ub tools to profile UCHL3's activity, we generate a quantitative kinetic model of the inhibitory mechanism and we find that K27 Ub 2 can inhibit UCHL3 covalently, by binding to its catalytic cysteine, and allosterically, by locking its catalytic loop tightly in place."
33238157
sparser
"As an additional control experiment, we measured a K i of 0.27 mM for the inhibition of UCH-L3 by ubiquitin with the substrate ubiquitin-AMC."
16905103
reach
"(2) The kinetics of inhibition of UCH-L3 by ubiquitin C-terminal aldehyde (Ub-H) were determined and reveal a Ki that is less than 10 (-14) M. Several mechanisms are considered to account for the extreme inhibition."
9485312
Ubiquitin increases the amount of UCHL3.
| 1
Ubiquitin increases the amount of UCHL3. 1 / 1
| 1
reach
"Therefore, ubiquitin aldehyde may modulate the protein expression and function of UCHL3."
27780264
UCHL3 affects Ubiquitin
| 3 35
UCHL3 binds Ubiquitin.
| 1 35
Ubiquitin binds UCHL3. 10 / 35
| 1 30
sparser
"Next we quantified the change in chemical shift induced upon binding of ubiquitin to UCH-L3."
10518943
sparser
"Having defined the surface of ubiquitin that interacts with UCH-L3, we next sought to identify candidate residues on UCH-L3 that interact with ubiquitin."
10518943
sparser
"The high preference at the P3 position and relatively low specificity in the P4 position can be explained by comparison with the published crystal structure of UCH-L3 bound with ubiquitin inhibitor (Fig.3) ."
18601651
sparser
"The high preference at the P3 position and relatively low specificity in the P4 position can be explained by comparison with the published crystal structure of UCH-L3 bound with ubiquitin inhibitor ( xref )."
18601651
sparser
"From a crystal structure of a complex between UCHL3 and synthetic K27 Ub 2 , we unexpectedly discover that free K27 Ub 2 and K27 Ub 2 -conjugated substrates are natural inhibitors of UCHL3."
33238157
sparser
"In contrast, when UCH-L3 is bound to an ubiquitin-based inhibitor, ubiquitin vinyl methyl ester, the crossover loop becomes stabilized in an α-helix followed by an S shaped loop forming contacts with the C terminus of ubiquitin ( xref )."
19489724
sparser
"To determine if these electrostatic interactions contribute significantly to the stability of the ubiquitin·UCH-L3 complex, we mutated a number of basic residues on the surface of ubiquitin and measur[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10518943
sparser
"Since ubiquitin binding orders a significant portion of the protein, the UCH domain of BAP1 was modeled after Uch-L3 bound to ubiquitin (PDB: 1xd3)."
22683710
sparser
"Information for structures was from the protein data bank (PDB): ubiquitin from UCH-L3-Ub complex (PDB code: 1XD3) and ISG15 (PDB code: 1Z2M)."
20153823
sparser
"Model showing binding of ubiquitin aldehyde to UCH-L3 using PDB accession 1XD3: blue -the size dominant exosite that binds epitopes on the ubiquitin surface, red -the smaller linear epitope corresponding to the C-terminal tetrapeptide (P2-P4) that binds the cleavage site cleft of the enzymes, purple -Arg at P5."
18601651
Ubiquitin binds UCHL1 and UCHL3. 3 / 3
| 3
sparser
"T218, R227, F228, and L230 residues of BAP1N were selected based on ubiquitin interaction with the corresponding residues of UCHL1 and UCHL3."
28935764
sparser
"In contrast with BAP1, the thermodynamics of ubiquitin binding to UCHL1 and UCHL3 is dominated by enthalpy factors and hydrogen bonding and ionic interactions that stabilize the complex."
28935764
sparser
"Unlike UCH-L1, UCH-L3 can interact with Lys48-linked Ub dimers (di-Ub) to protect it from degradation and meanwhile to inhibit its hydrolase activity."
20302916
Ubiquitin binds UCHL3 and LMO7. 1 / 1
| 1
sparser
"We suggest that the increase in severity may reflect an interaction between Uchl3 and Lmo7 in the ubiquitin-mediated protein degradation pathway."
12761045
Ubiquitin binds UCHL3 and NEDD8. 1 / 1
| 1
sparser
"In vitro GST pull-down assay demonstrated that UCH-L3 bound to both NEDD8 and ubiquitin."
9790970
UCHL3 inhibits Ubiquitin.
| 1
UCHL3 inhibits Ubiquitin. 1 / 1
| 1
reach
"Through application of a novel protein cleavage and tagging technique, sortagging, we show that human UCHL3 and the Plasmodium falciparum homologue, members of the ubiquitin C-terminal hydrolase family, use a unique active site crossover loop to restrict access of bulky ubiquitin adducts to the active site."
19047059
UCHL3 activates Ubiquitin.
| 1
UCHL3 activates Ubiquitin. 1 / 1
| 1
reach
"The relevant control exerted by these DUBs on ubiquitin homeostasis may account for these effects since pharmacological blocking of UCHL1 or UCHL3 reduced the monomeric ubiquitin pool, in turn restricting the ubiquitination of GlyT2 in neurons (XREF_FIG)."
23484054
UCHL3 affects COPS5
1 | 20 10
UCHL3 binds COPS5.
1 | 8 10
UCHL3 binds COPS5. 10 / 18
1 | 8 9
sparser
"Furthermore, we reveal that UCHL3 physically interacts with COPS5, and determines the level and protein stability of cellular COPS5 by deubiquitinating COPS5."
28583475
reach
"Given that we demonstrated that UCHL3 promoted activation of IFN signaling, we observed whether IFN-I could affect the interaction between UCHL3 and COPS5."
28583475
reach
"Next, we find that UCHL3 physically interacts with COPS5, and upregulates COPS5 levels and protein stability by deubiquitinating COPS5."
28583475
reach
"Furthermore, we reveal that UCHL3 physically interacts with COPS5, and determines the level and protein stability of cellular COPS5 by deubiquitinating COPS5."
28583475
sparser
"These results imply that UCHL3 physically interacts with COPS5 and deubiquitinates COPS5."
28583475
reach
"The result showed that IFNalpha promoted the binding of UCHL3 to COPS5 during the early stage of IFNalpha treatment, which is consistent with the effect of UCHL3 on TYK2 phosphorylation during the ear[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28583475
sparser
"We demonstrate that UCHL3 physically interacts with COPS5, and increases COPS5 levels and protein stability by deubiquitinating COPS5."
28583475
sparser
"Given that we demonstrated that UCHL3 promoted activation of IFN signaling, we observed whether IFN-I could affect the interaction between UCHL3 and COPS5."
28583475
reach
"Thus, we demonstrate that UCHL3 regulates Cullin1 neddylation dependently on COPS5.To further understand the relationship of UCHL3 and COPS5, we analyzed the interaction between UCHL3 and COPS5."
28583475
biogrid
No evidence text available
19615732
BTRC binds UCHL3, COPS5, CUL1, and KITLG. 1 / 1
| 1
sparser
"Interestingly, we find that UCHL3 regulates COPS5-dependent deneddylation of Cullin1, which is an essential component of SCF β-TrCP complex and associated with SCF β-TrCP activities."
28583475
UCHL3 increases the amount of COPS5.
| 5
UCHL3 bound to COPS5 increases the amount of COPS5. 2 / 2
| 2
reach
"We demonstrate that UCHL3 physically interacts with COPS5, and increases COPS5 levels and protein stability by deubiquitinating COPS5."
28583475
reach
"Next, we find that UCHL3 physically interacts with COPS5, and upregulates COPS5 levels and protein stability by deubiquitinating COPS5."
28583475
UCHL3 increases the amount of COPS5. 2 / 2
| 2
reach
"Furthermore, we demonstrate that UCHL3 as a deubiquitinase can deubiquitinate COPS5, thereby upregulating COPS5 levels and protein stability (Figs. 4 and 5)."
28583475
reach
"In summary, our results demonstrate that UCHL3 upregulates the levels of SCF beta-TrCP substrates.We have demonstrated that UCHL3 positively regulated COPS5 levels and IFNAR1 levels."
28583475
Modified UCHL3 increases the amount of COPS5. 1 / 1
| 1
reach
"Meanwhile, endogenous COPS5 levels were also gradually upregulated by overexpression of increased amounts of UCHL3."
28583475
UCHL3 deubiquitinates COPS5.
| 4
UCHL3 deubiquitinates COPS5. 4 / 4
| 4
reach
"To address this question, we firstly performed an in vitro deubiquitination assay, which clearly demonstrated that UCHL3 can deubiquitinate COPS5 in vitro."
28583475
reach
"These results imply that UCHL3 physically interacts with COPS5 and deubiquitinates COPS5.Given that UCHL3 can deubiquitinate COPS5, we speculate that UCHL3 could affect cellular levels of COPS5."
28583475
reach
"Given that UCHL3 interacts with COPS5 in IFN signaling and UCHL3 is a deubiquitinase, we question whether UCHL3 promotes COPS5 deubiquitination."
28583475
reach
"Furthermore, we demonstrate that UCHL3 as a deubiquitinase can deubiquitinate COPS5, thereby upregulating COPS5 levels and protein stability (Figs. 4 and 5)."
28583475
UCHL3 decreases the amount of COPS5.
| 2
UCHL3 decreases the amount of COPS5. 2 / 2
| 2
reach
"We found that UCHL3 knockdown noticeably upregulated ubiquitination levels of COPS5."
28583475
reach
"Since COPS5 have been demonstrated to downregulate Cullin1 neddylation and activity, we speculate that UCHL3 could downregulate the activity of SCF beta and TrCP complex by upregulating cellular COPS5[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28583475
UCHL3 activates COPS5.
| 1
UCHL3 activates COPS5. 1 / 1
| 1
reach
"The result showed that FH-UCHL3 overexpression obviously blocked protein degradation of COPS5, suggesting that UCHL3 enhances COPS5 protein stability."
28583475
UCHL3 is modified
7 | 1 23
UCHL3 is phosphorylated. 10 / 20
| 1 19
sparser
"Lastly, we provide evidence showing that DNA damage-induced UCHL3 phosphorylation (which is dependent on UCHL3 catalytic activity, ATM kinase and downstream NHEJ factors) promotes destabilization of UCHL3, suggesting that UCHL3 may dissociate from Ku80 to allow Ku ubiquitylation and subsequent removal from DNA."
30559450
sparser
"Accordingly, the interaction of Ku80 and UCHL3, which requires UCHL3 catalytic activity (Fig.  xref ), was not markedly affected when FLAG-tagged phospho-mutants of UCHL3 were examined by immunoprecipitation (Supplementary Fig.  xref )."
30559450
sparser
"Indeed, DNA damage-induced UCHL3 phosphorylation was not detected with enzymatically inactive UCHL3 (Fig.  xref ), suggesting that UCHL3 could be phosphorylated at sites of DNA damage."
30559450
sparser
"Obviously, the biological role and mechanism of UCHL3 phosphorylation has not been fully understood yet, and this requires further research ( xref )."
34177595
sparser
"To further establish that the signal detected with the anti-phospho S/TQ antibody indicates phosphorylated UCHL3, immunoblotting was performed with a mutant UCHL3 in which Ser75 was substituted with Ala (S75A)."
30559450
sparser
"Mechanistically, we show that DNA damage induces UCHL3 phosphorylation, which is dependent on ATM, downstream NHEJ factors and UCHL3 catalytic activity."
30559450
sparser
"The phosphorylation of UCHL3 was also induced by IR treatment (data not shown)."
30559450
sparser
"Mechanistically, in response to DNA damage, the deubiquitinase UCHL3 is phosphorylated and activated by ATM."
27941124
sparser
"Here, we first analyzed the structure of simulated phosphorylated UCHL3 S75E and the complex of UCHL3 S75E with Ub-PA and preliminarily explained the structural mechanism of phosphorylation-enhanced UCHL3 deubiquitinating activity."
36142702
sparser
"These data indicate that phosphorylation of UCHL3 at sites of DNA damage requires downstream c-NHEJ factors."
30559450
UCHL3 is phosphorylated on S75. 6 / 6
2 | 4
hprd
No evidence text available
20068231
sparser
"UCHL3 is phosphorylated on S75 in a manner dependent on its catalytic activity, ATM and XRCC4 (as well as LIG4)."
30559450
sparser
"The ensuing results demonstrated that the S75A mutation abolished the signal from the anti-phospho-S/TQ antibody, thus confirming that Ser75 of UCHL3 is phosphorylated in response to DSBs (Supplementary Fig.  xref )."
30559450
sparser
"UCHL3 is phosphorylated at Ser75 and activated by ATM upon DNA damage."
34177595
hprd
No evidence text available
17525332
sparser
"Altogether, these data suggest that phosphorylation of UCHL3 on S75, which is dependent on ATM, UCHL3′s catalytic activity and downstream NHEJ factors such as XRCC4 and LigaseIV, regulates Ku80 ubiquitylation by regulating UCHL3 stability rather than affecting its deubiquitylating activity after DSB induction."
30559450
UCHL3 is phosphorylated on S130. 2 / 2
2 |
hprd
No evidence text available
18669648
hprd
No evidence text available
20166139
UCHL3 is phosphorylated on S82. 1 / 1
1 |
hprd
No evidence text available
20068231
UCHL3 is phosphorylated on T81. 1 / 1
1 |
hprd
No evidence text available
20068231
UCHL3 is phosphorylated on Y85. 1 / 1
1 |
hprd
No evidence text available
20068231
UCHL3 affects AHR
| 17 3
UCHL3 activates AHR.
| 8
UCHL3 activates AHR. 7 / 7
| 7
reach
"In summary, our findings revealed that AhR plays a critical role in maintaining the stem-like properties of cells overexpressing UCHL3, confirming that UCHL3 promotes tumor stem-like properties through stabilizing AhR."
32546741
reach
"UCHL3 increases AhR protein stability in a DUB activity-dependent manner."
32546741
reach
"Generally, these findings indicated that as a DUB of AhR, UCHL3 increases AhR protein stabilization in a DUB activity-dependent manner."
32546741
reach
"In summary, our experiments indicate that UCHL3 is a specific DUB for AhR and that UCHL3 elevates AhR protein stabilization through deubiquitination.We further detected mRNA levels in 35 paired ADC and SCC lung cancer and corresponding paracancerous normal tissues (Supplementary Fig. S4b, c) and noted no difference between histologic subtypes."
32546741
reach
"Collectively, these findings indicate that inhibition of UCHL3 may effectively eliminate lung cancer stem cell properties by promoting AhR destabilization."
32546741
reach
"In this paper, we found a new mechanism of AhR regulation and functional mechanism by which UCHL3 promotes lung cancer progression and stemness."
32546741
reach
"UCHL3 increased AhR stability and the binding of AhR to the promoter regions of the "stemness" genes ATP-binding cassette subfamily G member 2 (ABCG2), KLF4, and c-Myc."
32546741
Mutated UCHL3 activates AHR. 1 / 1
| 1
reach
"Nevertheless, our results suggest that WT UCHL3, but not UCHL3 mutants, promotes tumor growth and stem-like properties through stabilizing AhR in a DUB activity-dependent manner."
32546741
UCHL3 deubiquitinates AHR.
| 3
UCHL3 deubiquitinates AHR. 3 / 3
| 3
reach
"As expected, the ability of UCHL3 to deubiquitinate AhR was almost completely abrogated by TCID treatment (Fig. 7a)."
32546741
reach
"WT UCHL3, but not UCHL3-C95S, significantly reduced AhR protein polyubiquitination (Fig. 6f), whereas the other mutants did not decrease AhR polyubiquitination."
32546741
reach
"Second, UCHL3 reduces AhR polyubiquitination and enhances AhR protein stabilization in a DUB activity-dependent manner, and this ability could be inhibited by TCID, a UCHL3 inhibitor."
32546741
UCHL3 binds AHR.
| 3
UCHL3 binds AHR. 3 / 3
| 3
sparser
"UCHL3 interacts with AhR and stabilizes the AhR protein through deubiquitination."
32546741
sparser
"Then, we explored the interaction between the UCHL3 and AhR proteins."
32546741
sparser
"First, UCHL3 and AhR directly interact."
32546741
UCHL3 ubiquitinates AHR.
| 2
UCHL3 leads to the ubiquitination of AHR. 2 / 2
| 2
reach
"Correspondingly, TCID treatment reduced AhR protein levels and stemness-associated markers in UCHL3-overexpressing A549 cells without affecting AhR mRNA levels (Fig. 7b, c), suggesting that TCID, like UCHL3 knockdown, promotes AhR ubiquitination and degradation."
32546741
reach
"WT UCHL3, but not UCHL3-C95S, significantly reduced AhR protein polyubiquitination (Fig. 6f), whereas the other mutants did not decrease AhR polyubiquitination."
32546741
UCHL3 increases the amount of AHR.
| 2
UCHL3 increases the amount of AHR. 2 / 2
| 2
reach
"WT UCHL3 obviously increased AhR and c-Myc protein levels, whereas the mutants did not (Fig. 6a)."
32546741
reach
"Furthermore, overexpression of UCHL3 decreased the ubiquitination of endogenous AhR in A549 and H1299 cells (Fig. 4h, i), while depletion of endogenous UCHL3 elevated the ubiquitination level of endogenous AhR in H358 cells (Fig. 4j)."
32546741
UCHL3 inhibits AHR.
| 1
UCHL3 inhibits AHR. 1 / 1
| 1
reach
"UCHL3 reduced the radiosensitivity of NSCLC cells by stabilizing AhR protein."
35918714
UCHL3 decreases the amount of AHR.
| 1
UCHL3 decreases the amount of AHR. 1 / 1
| 1
reach
"Furthermore, overexpression of UCHL3 decreased the ubiquitination of endogenous AhR in A549 and H1299 cells (Fig. 4h, i), while depletion of endogenous UCHL3 elevated the ubiquitination level of endogenous AhR in H358 cells (Fig. 4j)."
32546741
COPS5 affects UCHL3
1 | 9 10
COPS5 binds UCHL3.
1 | 8 10
UCHL3 binds COPS5. 10 / 18
1 | 8 9
sparser
"Furthermore, we reveal that UCHL3 physically interacts with COPS5, and determines the level and protein stability of cellular COPS5 by deubiquitinating COPS5."
28583475
reach
"Given that we demonstrated that UCHL3 promoted activation of IFN signaling, we observed whether IFN-I could affect the interaction between UCHL3 and COPS5."
28583475
reach
"Next, we find that UCHL3 physically interacts with COPS5, and upregulates COPS5 levels and protein stability by deubiquitinating COPS5."
28583475
reach
"Furthermore, we reveal that UCHL3 physically interacts with COPS5, and determines the level and protein stability of cellular COPS5 by deubiquitinating COPS5."
28583475
sparser
"These results imply that UCHL3 physically interacts with COPS5 and deubiquitinates COPS5."
28583475
reach
"The result showed that IFNalpha promoted the binding of UCHL3 to COPS5 during the early stage of IFNalpha treatment, which is consistent with the effect of UCHL3 on TYK2 phosphorylation during the ear[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28583475
sparser
"We demonstrate that UCHL3 physically interacts with COPS5, and increases COPS5 levels and protein stability by deubiquitinating COPS5."
28583475
sparser
"Given that we demonstrated that UCHL3 promoted activation of IFN signaling, we observed whether IFN-I could affect the interaction between UCHL3 and COPS5."
28583475
reach
"Thus, we demonstrate that UCHL3 regulates Cullin1 neddylation dependently on COPS5.To further understand the relationship of UCHL3 and COPS5, we analyzed the interaction between UCHL3 and COPS5."
28583475
biogrid
No evidence text available
19615732
BTRC binds UCHL3, COPS5, CUL1, and KITLG. 1 / 1
| 1
sparser
"Interestingly, we find that UCHL3 regulates COPS5-dependent deneddylation of Cullin1, which is an essential component of SCF β-TrCP complex and associated with SCF β-TrCP activities."
28583475
COPS5 activates UCHL3.
| 1
COPS5 activates UCHL3. 1 / 1
| 1
reach
"However, we found that knockout of COPS5 abolished the effect of UCHL3 on Cullin1 deneddylation, indicating that COPS5 is required for UCHL3 mediated deneddylation of Cullin1 in cells."
28583475
reach
"UCH-L3 was found to be downregulated in the highly metastatic prostate cell lines, and knockdown of UCH-L3 induced EMT process."
28279784
reach
"The reverse of the EMT event caused by UCH-L3 overexpression in PC3 coincided with a decrease of cell migration and invasion.UCH-L3 and UCH-L1 are very close relatives with high amino acid identity an[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25194810
reach
"As shown in Fig. 2 B, knockdown of UCH-L3 induces the molecular alterations of EMT."
25194810
reach
"These results indicate that UCH-L3 inhibits EMT by repression of EMT promoting genes such as Snail, Slug, Twist, and MMPs in normal prostate cell lines.In our previous report, we found that UCH-L1 exp[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25194810
reach
"We found that knockdown of UCH-L3 induced EMT process."
25194810
reach
"In our study, ablation of the UCH-L3 deubiquitinating activity did not inhibit EMT and it did not reduce cancer cell migration and invasion in PC3."
25194810
reach
"The induction of EMT by UCH-L3 knockdown results in an increase of cell migration and invasion."
25194810
reach
"Contrarily, overexpression of UCH-L3 in the highly metastatic prostate cell line PC3 reverses EMT depending on the deubiquitinating activity of UCH-L3."
25194810
reach
"Yet, to the contrary, overexpression of UCH-L3 in highly metastatic prostate cancer cell line PC3 reverses EMT but the active site mutant UCH-L3 did not."
25194810
eidos
"By contrast , UCHL3 is reduced in metastatic prostate cancer cell lines , and knockdown of UCHL3 promotes epithelial-to-mesenchymal transition ( EMT ) , contributing to cancer cell invasion and metastasis ( 102 ) ."
33585209
reach
"Knockdown of UCH-L3 in the normal prostate cell line RWPE1 induces morphological and molecular events of EMT."
25194810
reach
"We have already shown that UCH-L1, a close relative of UCH-L3, induces EMT and promotes metastasis in prostate cancer cells [11]."
25194810
UCHL3 increases the amount of epithelial to mesenchymal transition.
| 1
Modified UCHL3 increases the amount of epithelial to mesenchymal transition. 1 / 1
| 1
reach
"The alterations of the EMT marker expression induced by UCH-L3 overexpression in PC3 stable cells coincided with the change of migratory and invasive properties."
25194810
UCHL3 affects TDP1
| 11 3
UCHL3 binds TDP1.
| 1 3
UCHL3 binds TDP1. 4 / 4
| 1 3
reach
"Furthermore, incubation of purified K48 ubiquitylated TDP1 with recombinant UCHL3 led to reduction of TDP1 ubiquitylation (XREF_FIG B) and a physical interaction between TDP1 and UCHL3 was observed by co-immunoprecipitation (XREF_FIG C)."
29898404
sparser
"Furthermore, incubation of purified K48-ubiquitylated TDP1 with recombinant UCHL3 led to reduction of TDP1 ubiquitylation ( xref B) and a physical interaction between TDP1 and UCHL3 was observed by co-immunoprecipitation ( xref C)."
29898404
sparser
"Here, we report multiple evidence showing a direct role of UCHL3 in controlling TDP1 proteostasis: (1) reduction of K48-ubiquitylated TDP1 in cancer cells overexpressing UCHL3, (2) reduction of K48-uibiquitylated TDP1 upon addition of recombinant UCHL3, (3) enrichment of K48-ubiquitylated TDP1 upon MG132 treatment, which is dependent on UCHL3 levels, (4) epistatic relationship showing that co-depletion of TDP1 and UCHL3 does not further sensitize cells to TOP1 poisons than depletion of either enzyme alone, (5) physical interaction between TDP1 and UCHL3, and (6) changes in the ubiquitylation status and TDP1 turnover upon changes in UCHL3 levels induced by its ectopic overexpression, siRNA depletion, CRISPR editing, and pharmacologic inhibition and, also, in two physiologically relevant clinical settings."
29898404
sparser
"We show that R586 methylation promotes TDP1 ubiquitylation, which facilitates ubiquitin/proteasome-dependent TDP1 turnover by impeding the binding of UCHL3 (deubiquitylase enzyme) with TDP1."
35705029
UCHL3 ubiquitinates TDP1.
| 3
UCHL3 leads to the ubiquitination of TDP1. 3 / 3
| 3
reach
"Depletion of UCHL3 increases TDP1 ubiquitylation and turnover rate and sensitizes cells to TOP1 poisons."
29898404
reach
"In contrast, UCHL3 is downregulated in spinocerebellar ataxia with axonal neuropathy (SCAN1), causing elevated levels of TDP1 ubiquitylation and faster turnover rate."
29898404
reach
"To examine if the increased TDP1 ubiquitylation caused by UCHL3 depletion would lead to increased turnover, we monitored the TDP1 protein level following incubations with the protein synthesis inhibitor cycloheximide."
29898404
UCHL3 decreases the amount of TDP1.
| 3
UCHL3 decreases the amount of TDP1. 3 / 3
| 3
reach
"Depletion of UCHL3 was sufficient to reduce TDP1 expression in rhabdomyosarcoma cells to levels comparable to control cells (XREF_FIG D, top) and led to hypersensitivity to the TOP1 poison CPT (XREF_FIG D, bottom)."
29898404
reach
"UCHL3 decreases the ubiquitination level of TDP1 leading to the inhibition of TDP1 degradation."
31967940
reach
"Depletion of UCHL3 in rhabdomyosarcoma cells reduced TDP1 levels and sensitized cells to TOP1 poisons."
29898404
UCHL3 deubiquitinates TDP1.
| 2
UCHL3 leads to the deubiquitination of TDP1. 1 / 1
| 1
reach
"In contrast, UCHL3 is downregulated in spinocerebellar ataxia with axonal neuropathy (SCAN1), causing elevated levels of TDP1 ubiquitylation and faster turnover rate."
29898404
Modified UCHL3 leads to the deubiquitination of TDP1. 1 / 1
| 1
reach
"Overexpression of UCHL3, but not a catalytically inactive mutant, suppresses TDP1 ubiquitylation and turnover rate."
29898404
UCHL3 increases the amount of TDP1.
| 1
Modified UCHL3 increases the amount of TDP1. 1 / 1
| 1
reach
"TDP1 overexpression in the topoisomerase therapy resistant rhabdomyosarcoma is driven by UCHL3 overexpression."
29898404
UCHL3 activates TDP1.
| 1
UCHL3 activates TDP1. 1 / 1
| 1
reach
"Depletion of UCHL3 in rhabdomyosarcoma cells markedly accelerated TDP1 turnover, as measured by CHX chase experiments (XREF_FIG B)."
29898404
UCHL3 affects UBC
13 |
UBC binds UCHL3. 10 / 13
13 |
biogrid
No evidence text available
28190767
biogrid
No evidence text available
14673145
biogrid
No evidence text available
20622874
biogrid
No evidence text available
10518943
biogrid
No evidence text available
27066941
biogrid
No evidence text available
9790970
biogrid
No evidence text available
22284438
biogrid
No evidence text available
15531586
biogrid
No evidence text available
25590432
biogrid
No evidence text available
23801757
UCHL3 affects RAD51
4 1 | 7 1
UCHL3 deubiquitinates RAD51.
1 | 6
UCHL3 deubiquitinates RAD51. 7 / 7
1 | 6
reach
"UCHL3 deubiquitinates RAD51 thereby promoting the recruitment of RAD51 to DNA damage sites and augmenting DNA repair."
31113933
reach
"After DNA damages, UCHL3 is activated via phosphorylation by ATM and then deubiquitinates RAD51 promoting RAD51-BRCA2 interaction and proper HR repair [150]."
28552167
ubibrowser
"UCHL3, in turn, deubiquitinates RAD51 and promotes the binding between RAD51 and BRCA2."
27941124
reach
"UCHL3 deubiquitylates RAD51 to promote the interaction between RAD51 and BRCA2 and HR repair [121]."
33990032
reach
"RAD51 deubiquitination by UCHL3 was observed both in vitro and in cellulo and shown to be critical for BRCA2-RAD51 interaction, RAD51 recruitment to DSB sites, foci formation, as well as RAD51 mediated D-loop formation [XREF_BIBR]."
| PMC
reach
"found that UCHL3 deubiquitinates RAD51 and subsequently facilitates RAD51-BRCA2 interaction, which is critical for homologous recombination (HR) and contributes to therapeutic resistance in breast cancer."
33585209
reach
"UCHL3, in turn, deubiquitinates RAD51 and promotes the binding between RAD51 and BRCA2."
27941124
UCHL3 binds RAD51.
4 | 1 1
UCHL3 binds RAD51. 6 / 6
4 | 1 1
biogrid
No evidence text available
27941124
sparser
"UCHL3 interacts with RAD51 and de-ubiquitinates residues that disrupt the RAD51–BRCA2 interaction."
31769469
biogrid
No evidence text available
27941124
reach
"Consist with this notion, it is suggested that UCHL3 interacts and deubiquitylates RAD51, which is required for the efficient HR [104]."
28764946
biogrid
No evidence text available
27941124
biogrid
No evidence text available
27941124
UBC affects UCHL3
13 |
UBC binds UCHL3. 10 / 13
13 |
biogrid
No evidence text available
28190767
biogrid
No evidence text available
14673145
biogrid
No evidence text available
20622874
biogrid
No evidence text available
10518943
biogrid
No evidence text available
27066941
biogrid
No evidence text available
9790970
biogrid
No evidence text available
22284438
biogrid
No evidence text available
15531586
biogrid
No evidence text available
25590432
biogrid
No evidence text available
23801757
UCHL3 affects UCHL1
| 4 8
UCHL3 binds UCHL1.
| 8
UCHL1 binds UCHL3. 5 / 5
| 5
sparser
"To identify the proteins that interact with UCH-L1 or UCH-L3, which might represent the potential substrates or the functional effecters, we performed immunoaffinity chromatography."
19154770
sparser
"This resulted in UCHL3:UCHL1 selectivity for UbV Q40V/T66K/V70F -PRG of approximately 20,000-fold, almost doubling the selectivity ratio described above for UbV T66K/V70F -PRG (10,700-fold)."
35053210
sparser
"Nedd8, an Ub-like protein with 57% identity to Ub, is a previously reported UCH-L3 and UCH-L1 interacting protein ( Wada et al., 1998; Hemelaar et al., 2004 )."
19154770
sparser
"Thus, the interactions of di-Ub with UCH-L3 and mono-Ub with UCH-L1 occupy the catalytic centers of UCH-L3, and UCH-L1, respectively."
19154770
sparser
"In addition, the increased levels of both UCH-L1 and UCH-L3 mRNA are associated with early tumor recurrence of invasive breast cancer and poor prognosis [55] ."
20302916
Ubiquitin binds UCHL1 and UCHL3. 3 / 3
| 3
sparser
"T218, R227, F228, and L230 residues of BAP1N were selected based on ubiquitin interaction with the corresponding residues of UCHL1 and UCHL3."
28935764
sparser
"In contrast with BAP1, the thermodynamics of ubiquitin binding to UCHL1 and UCHL3 is dominated by enthalpy factors and hydrogen bonding and ionic interactions that stabilize the complex."
28935764
sparser
"Unlike UCH-L1, UCH-L3 can interact with Lys48-linked Ub dimers (di-Ub) to protect it from degradation and meanwhile to inhibit its hydrolase activity."
20302916
UCHL3 inhibits UCHL1.
| 2
UCHL3 inhibits UCHL1. 2 / 2
| 2
reach
"The screening of over 40 000 compounds against the closely related ubiquitin Cterminal hydrolase L1 (UCH-L1) and L3 (UCH-L3) enzymes allowed the development of a UCHL1-specific inhibitor based on an isatin O-acyl oxime scaffold [105] ."
28052575
reach
"We found that UCH-L1 is highly induced by UCH-L3 knockdown in RWPE1."
25194810
UCHL3 decreases the amount of UCHL1.
| 1
UCHL3 decreases the amount of UCHL1. 1 / 1
| 1
reach
"Interestingly, we found that knockdown of UCH-L3 induces the expression of UCH-L1 which is repressed by epigenetic modification in RWPE1."
25194810
UCHL3 activates UCHL1.
| 1
UCHL3 activates UCHL1. 1 / 1
| 1
reach
"UCHL1 and UCHL3 are 73% similar but display different patterns of tissue specific gene expression : an upstream neuron-restrictive silencing element (NRSE) drives neuron specific expression in the former and renders it a critical player in neuronal homeostasis (UCHL1 deficiency in neurodegeneration is not physiologically rescued by UCHL3)."
28177072
UCHL3 affects NEDD8
5 1 | 3 2
UCHL3 binds NEDD8.
5 1 | 2 2
UCHL3 binds NEDD8. 8 / 9
5 1 | 2
hprd
No evidence text available
9790970
biogrid
No evidence text available
16503656
reach
"UCH-L3, one of the ubiquitin C-terminal hydrolases, also binds to NEDD8 and functions as a NEDD8 C-terminal hydrolase (Wada et al., 1998)."
10806345
biogrid
No evidence text available
14673145
reach
"In vitro GST pull-down assay demonstrated that UCH-L3 bound to both NEDD8 and ubiquitin."
9790970
biogrid
No evidence text available
9790970
biogrid
No evidence text available
9790970
biogrid
No evidence text available
9790970
Ubiquitin binds UCHL3 and NEDD8. 1 / 1
| 1
sparser
"In vitro GST pull-down assay demonstrated that UCH-L3 bound to both NEDD8 and ubiquitin."
9790970
UCHL3 binds USP4 and NEDD8. 1 / 1
| 1
sparser
"NEDD8, UCHL3 and USP4 are highly associated with ubiquitination."
24093944
UCHL3 activates NEDD8.
| 1
UCHL3 activates NEDD8. 1 / 1
| 1
reach
"It was reported that UCHL3 enables to cleave the C-terminus of NEDD8, which is a ubiquitin like protein that exerts the function of Ub to be conjugated to a lysine residue of the substrate."
33585209
UCHL3 affects XRCC5
| 8 3
UCHL3 binds XRCC5.
| 2 3
UCHL3 binds XRCC5. 5 / 5
| 2 3
sparser
"Accordingly, the interaction of Ku80 and UCHL3, which requires UCHL3 catalytic activity (Fig.  xref ), was not markedly affected when FLAG-tagged phospho-mutants of UCHL3 were examined by immunoprecipitation (Supplementary Fig.  xref )."
30559450
reach
"Accordingly, the interaction of Ku80 and UCHL3, which requires UCHL3 catalytic activity (Fig. 1E), was not markedly affected when FLAG-tagged phospho-mutants of UCHL3 were examined by immunoprecipitation (Supplementary Fig. S7B)."
30559450
reach
"Here we found that UCHL3, a deubiquitylating enzyme, interacts with and can directly deubiquitylate Ku80 in a catalytic activity dependent manner."
30559450
sparser
"UCHL3 interacts with Ku80 and is recruited to DNA damage sites in a manner dependent on its catalytic activity."
30559450
sparser
"Given the findings that UCHL3 interacted with Ku80 and was recruited to sites of damage in a catalytic activity dependent manner (Fig.  xref , Supplementary Fig.  xref ), we investigated whether catalytic activity is required for its phosphorylation."
30559450
UCHL3 deubiquitinates XRCC5.
| 4
UCHL3 deubiquitinates XRCC5. 4 / 4
| 4
reach
"Taken together, these data suggest that UCHL3 could enhance Ku chromatin retention upon DSB induction by deubiquitylating Ku80."
30559450
reach
"UCHL3 de-ubiquitinates and stabilises Ku80 which is required for Ku80 retention at DSBs [10]."
31769469
reach
"Here we found that UCHL3, a deubiquitylating enzyme, interacts with and can directly deubiquitylate Ku80 in a catalytic activity dependent manner."
30559450
reach
"UCHL3 enhances Ku80 chromatin binding and directly deubiquitylates Ku80."
30559450
UCHL3 ubiquitinates XRCC5.
| 1
UCHL3 leads to the ubiquitination of XRCC5. 1 / 1
| 1
reach
"Since it was reported that Ku ubiquitylation is a prerequisite for Ku removal from chromatin, we hypothesized that UCHL3 may enhance Ku80 chromatin binding by antagonizing Ku80 ubiquitylation."
30559450
UCHL3 activates XRCC5.
| 1
UCHL3 activates XRCC5. 1 / 1
| 1
reach
"In accordance with reduced Ku80 IRIF, we observed that knockout and knockdown of UCHL3 reduced DSB-induced chromatin binding of Ku80 using chromatin fractionation until 3 hours after phleomycin removal (Fig. 4C and Supplementary Fig. S5)."
30559450
UCHL3 affects SMAD1
2 1 | 3 4
UCHL3 binds SMAD1.
2 | 2 4
UCHL3 binds SMAD1. 8 / 9
2 | 2 4
biogrid
No evidence text available
21453705
sparser
"For example, UCHL3 physically interacts with Smad1 and decreases its poly-ubiquitylation ( xref )."
29898404
reach
"We found that Uch-L3 physically interacts with Smad1 and dramatically decreases the amount of poly-ubiquitinated Smad1."
21453705
biogrid
No evidence text available
21453705
sparser
"UCH-L3 physically interacts with Smad1 and stabilizes it by deubiquitylating its polyubiquitin."
30123285
reach
"For example, UCHL3 physically interacts with Smad1 and decreases its poly-ubiquitylation."
29898404
sparser
"We found that Uch-L3 physically interacts with Smad1 and dramatically decreases the amount of poly-ubiquitinated Smad1."
21453705
sparser
"At the mechanistic level, we showed that SMAD1 interacted with UCHL3 in BMSCs and was downregulated when UCHL3 was suppressed."
36915132
UCHL3 deubiquitinates SMAD1.
1 |
UCHL3 deubiquitinates SMAD1. 1 / 1
1 |
ubibrowser
"Ubiquitin C-terminal hydrolase-L3 regulates Smad1 ubiquitination and osteoblast differentiation."
21453705
UCHL3 decreases the amount of SMAD1.
| 1
UCHL3 bound to SMAD1 decreases the amount of ubiquitinated SMAD1. 1 / 1
| 1
reach
"We found that Uch-L3 physically interacts with Smad1 and dramatically decreases the amount of poly-ubiquitinated Smad1."
21453705
NEDD8 affects UCHL3
5 1 | 2 2
UCHL3 binds NEDD8. 8 / 9
5 1 | 2
hprd
No evidence text available
9790970
biogrid
No evidence text available
16503656
reach
"UCH-L3, one of the ubiquitin C-terminal hydrolases, also binds to NEDD8 and functions as a NEDD8 C-terminal hydrolase (Wada et al., 1998)."
10806345
biogrid
No evidence text available
14673145
reach
"In vitro GST pull-down assay demonstrated that UCH-L3 bound to both NEDD8 and ubiquitin."
9790970
biogrid
No evidence text available
9790970
biogrid
No evidence text available
9790970
biogrid
No evidence text available
9790970
Ubiquitin binds UCHL3 and NEDD8. 1 / 1
| 1
sparser
"In vitro GST pull-down assay demonstrated that UCH-L3 bound to both NEDD8 and ubiquitin."
9790970
UCHL3 binds USP4 and NEDD8. 1 / 1
| 1
sparser
"NEDD8, UCHL3 and USP4 are highly associated with ubiquitination."
24093944
UCHL3 affects Neoplasms
| 10
UCHL3 activates Neoplasms. 9 / 9
| 9
reach
"Overexpression of UCHL3 promotes cell growth, colony formation, tumor formation, and tumor stem-like properties."
32546741
reach
"UCHL3 promotes tumor stem-like properties through AhR."
32546741
reach
"The mechanism by which UCHL3 promotes tumor stem-like properties through AhR requires further investigation.In conclusion, UCHL3 is an AhR DUB that promotes lung cancer proliferation, tumor growth and tumor stem-like properties through stabilizing AhR by its deubiquitination."
32546741
reach
"Ubiquitin carboxyl-terminal hydrolase L3 (UCHL3) has been reported to promote diverse tumors, but little is known about its role in HCC."
36969045
reach
"In summary, our findings revealed that AhR plays a critical role in maintaining the stem-like properties of cells overexpressing UCHL3, confirming that UCHL3 promotes tumor stem-like properties through stabilizing AhR."
32546741
reach
"Importantly, stable knockdown of UCHL3 suppressed tumor growth from H358 cells and inhibited tumor stem-like properties."
32546741
reach
"Finally, UCHL3 functions to promote tumor growth and lung cancer stem-like properties through AhR.Disorder in the deubiquitination process is usually related to tumorigenesis."
32546741
reach
"Compared with the injection of vector-transfected A549 cells, injection of UCHL3-overexpressing A549 cells significantly increased tumor size, volume, and weight after 1 month of growth (Fig. 2l–n), while the overall body weight was maintained (Supplementary Fig. S2c)."
32546741
reach
"In this study, we showed that UCHL3 overexpression increased tumor stem-like properties."
32546741
Mutated UCHL3 activates Neoplasms. 1 / 1
| 1
reach
"Nevertheless, our results suggest that WT UCHL3, but not UCHL3 mutants, promotes tumor growth and stem-like properties through stabilizing AhR in a DUB activity-dependent manner."
32546741
UCHL3 affects Neoplasm Metastasis
| 1 7
UCHL3 activates Neoplasm Metastasis.
| 1 6
reach
"Our findings showed that knockdown of UCHL3 inhibited cell growth, migration and invasion in cervical cancer cells while UCHL3 knockdown inhibited cervical cancer development and metastasis in vivo in mouse models."
36527747
reach
"UCHL3 depletion significantly decreased ATC progression, stem-like and metastasis, and increased cell sensitivity to chemotherapy."
36813921
reach
"UCHL3 stimulates metastasis of gastric cancer through upregulating IGF2."
34278751
reach
"UCHL3 promotes cervical cancer development and metastasis by stabilizing NRF2 via deubiquitination."
36527747
eidos
"UCHL3 promotes cervical cancer development and metastasis by stabilizing NRF2 via deubiquitination ."
36527747
reach
"The deubiquitinating enzyme UCHL3 promotes anaplastic thyroid cancer progression and metastasis through Hippo signaling pathway."
36813921
reach
"Collectively, our findings suggest that UCHL3 promotes cervical cancer development and metastasis by stabilizing NRF2 via deubiquitination."
36527747
UCHL3 inhibits Neoplasm Metastasis.
| 1
reach
"Although the promotion of tumor progression by UCH-L3 has been identified in several reports (Miyoshi et al. 2006; Zhang et al. 2020), a previous study reported that UCH-L3 suppressed cancer metastasis through EMT inhibition in prostate cancer cells (Song et al. 2014)."
34745437
SMAD1 affects UCHL3
2 | 2 4
UCHL3 binds SMAD1. 8 / 9
2 | 2 4
biogrid
No evidence text available
21453705
sparser
"For example, UCHL3 physically interacts with Smad1 and decreases its poly-ubiquitylation ( xref )."
29898404
reach
"We found that Uch-L3 physically interacts with Smad1 and dramatically decreases the amount of poly-ubiquitinated Smad1."
21453705
biogrid
No evidence text available
21453705
sparser
"UCH-L3 physically interacts with Smad1 and stabilizes it by deubiquitylating its polyubiquitin."
30123285
reach
"For example, UCHL3 physically interacts with Smad1 and decreases its poly-ubiquitylation."
29898404
sparser
"We found that Uch-L3 physically interacts with Smad1 and dramatically decreases the amount of poly-ubiquitinated Smad1."
21453705
sparser
"At the mechanistic level, we showed that SMAD1 interacted with UCHL3 in BMSCs and was downregulated when UCHL3 was suppressed."
36915132
Valproic acid affects UCHL3
8 |
Valproic acid increases the amount of UCHL3. 8 / 8
8 |
ctd
No evidence text available
27188386
ctd
No evidence text available
29154799
ctd
No evidence text available
24935251
ctd
No evidence text available
23179753
ctd
No evidence text available
25192806
ctd
No evidence text available
24383497
ctd
No evidence text available
28001369
ctd
No evidence text available
26272509
UCHL3 affects CUL1
| 7 1
UCHL3 inhibits CUL1.
| 4
UCHL3 inhibits CUL1. 4 / 4
| 4
reach
"We reveal that UCHL3 can inhibit Cullin1 neddylation in cells dependently on COPS5."
28583475
reach
"We found that overexpression of increased amounts of UCHL3 gradually inhibited endogenous Cullin1 neddylation, indicating that cellular Cullin1 neddylation are regulated by UCHL3 levels."
28583475
reach
"Our results showed that UCHL3 overexpression inhibits Cullin1 neddylation."
28583475
reach
"We find that in cells UCHL3 can decrease neddylation of Cullin1."
28583475
UCHL3 activates CUL1.
| 2
UCHL3 activates CUL1. 2 / 2
| 2
reach
"However, we found that knockout of COPS5 abolished the effect of UCHL3 on Cullin1 deneddylation, indicating that COPS5 is required for UCHL3 mediated deneddylation of Cullin1 in cells."
28583475
reach
"Subsequently, UCHL3 mediated upregulation of COPS5 promotes Cullin1 deneddylation."
28583475
UCHL3 decreases the amount of CUL1.
| 1
Modified UCHL3 decreases the amount of CUL1. 1 / 1
| 1
reach
"Our data showed that overexpression of UCHL3 in COPS5-wild type (WT) cells robustly decreased Cullin1 neddylation levels, consistent with our previous observation."
28583475
UCHL3 binds CUL1.
| 1
BTRC binds UCHL3, COPS5, CUL1, and KITLG. 1 / 1
| 1
sparser
"Interestingly, we find that UCHL3 regulates COPS5-dependent deneddylation of Cullin1, which is an essential component of SCF β-TrCP complex and associated with SCF β-TrCP activities."
28583475
UCHL1 affects UCHL3
| 8
UCHL1 binds UCHL3. 5 / 5
| 5
sparser
"To identify the proteins that interact with UCH-L1 or UCH-L3, which might represent the potential substrates or the functional effecters, we performed immunoaffinity chromatography."
19154770
sparser
"This resulted in UCHL3:UCHL1 selectivity for UbV Q40V/T66K/V70F -PRG of approximately 20,000-fold, almost doubling the selectivity ratio described above for UbV T66K/V70F -PRG (10,700-fold)."
35053210
sparser
"Nedd8, an Ub-like protein with 57% identity to Ub, is a previously reported UCH-L3 and UCH-L1 interacting protein ( Wada et al., 1998; Hemelaar et al., 2004 )."
19154770
sparser
"Thus, the interactions of di-Ub with UCH-L3 and mono-Ub with UCH-L1 occupy the catalytic centers of UCH-L3, and UCH-L1, respectively."
19154770
sparser
"In addition, the increased levels of both UCH-L1 and UCH-L3 mRNA are associated with early tumor recurrence of invasive breast cancer and poor prognosis [55] ."
20302916
Ubiquitin binds UCHL1 and UCHL3. 3 / 3
| 3
sparser
"T218, R227, F228, and L230 residues of BAP1N were selected based on ubiquitin interaction with the corresponding residues of UCHL1 and UCHL3."
28935764
sparser
"In contrast with BAP1, the thermodynamics of ubiquitin binding to UCHL1 and UCHL3 is dominated by enthalpy factors and hydrogen bonding and ionic interactions that stabilize the complex."
28935764
sparser
"Unlike UCH-L1, UCH-L3 can interact with Lys48-linked Ub dimers (di-Ub) to protect it from degradation and meanwhile to inhibit its hydrolase activity."
20302916
ATM affects UCHL3
1 1 | 1 1 1 1
ATM phosphorylates UCHL3.
1 | 1 1 1
ATM phosphorylates UCHL3. 3 / 4
| 1 1 1
reach
"Mechanistically, in response to DNA damage, the deubiquitinase UCHL3 is phosphorylated and activated by ATM."
27941124
sparser
"Additionally, ATM-induced phosphorylation of UCHL3 occurs after DNA damage, which in turn deubiquitinates RAD51 and promotes the binding between BRCA2 and RAD51 ( xref )."
34177595
rlimsp
"Mechanistically, in response to DNA damage, the deubiquitinase UCHL3 is phosphorylated and activated by ATM."
27941124
ATM phosphorylates UCHL3 on S75. 1 / 1
1 |
biopax:phosphositeplus
No evidence text available
ATM activates UCHL3.
| 1
ATM activates UCHL3. 1 / 2
| 1
trips
"Mechanistically, in response to DNA damage, the deubiquitinase UCHL3 is phosphorylated and activated by ATM."
27941124
ATM binds UCHL3.
1 |
UCHL3 binds ATM. 1 / 1
1 |
biogrid
No evidence text available
27941124
reach
"UCH-L3 promotes the differentiation of osteoblast from C2C12 cells, while knockdown of Uch-l3 delays osteoblast differentiation [73]."
30123285
reach
"These results suggest that Uch-L3 enhances osteoblast differentiation through the stabilization of Smad1 signaling."
21453705
reach
"Even though the function of UCH-L3 in neurons has not been elucidated to the extent of our knowledge, it may be plausible that there can be reciprocal or differential functions of UCH-L3 to the UCH-L1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25194810
reach
"Consequently, it was suggested that UCHL3 stabilizes Smad1 signaling, which may explain the enhanced osteoblast differentiation caused by UCHL3 overexpression."
29898404
Modified UCHL3 activates cell differentiation. 1 / 1
| 1
reach
"Moreover, ectopic expression of wild-type UCH-L3 restored the phosphorylation of insulin and IGF-I receptor and adipocyte differentiation in Uchl3 (-/-) MEFs."
19837878
UCHL3 affects IFN-I
| 7
UCHL3 activates IFN-I. 7 / 7
| 7
reach
"Furthermore, we reveal that UCHL3 increases levels of SCF beta-TrCP substrates and enhances IFN-I mediated signaling and antiviral activity."
28583475
reach
"This finding suggests that UCHL3 could target either TYK2 itself or IFN-I receptors (IFNAR1 and IFNAR2), which are the direct proteins upstream of TYK2 as well as the first signaling proteins in IFN-I[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28583475
reach
"If doing so, one could understand that UCHL3 promotes IFN-I signaling by deneddylation of Cullin1 and inhibition of SCF beta and TrCP complex activity.To test this hypothesis, cells were transfected w[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28583475
reach
"Interestingly, overexpression of UCHL3 robustly promoted IFN-I mediated antiviral activity, as shown by a significantly decreased VSV-GFP signaling."
28583475
reach
"Our data showed that knockdown of endogenous UCHL3 inhibited IFN-I induced transcription activation of all three ISGs, strongly suggesting that UCHL3 positively regulates IFN-I signaling pathway.Given[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28583475
reach
"Taken all together, our data suggest that UCHL3 positively regulates IFN-I mediated signaling pathway and antiviral activity.Deubiquitinases play important roles in controlling signaling pathways rela[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28583475
reach
"However, the roles of most of deubiquitinases in regulating IFN-I antiviral signaling are still largely unexplored.Here, we report that UCHL3 positively regulates IFN-I antiviral signaling pathway."
28583475
UCHL3 activates cell population proliferation. 5 / 5
| 5
reach
"Taken together, the results of the present study suggest that UCHL3 knockdown decreases melanoma cell proliferation by increasing cell autophagy through regulating NEDD8 expression and autophagosome numbers."
34539860
reach
"UCH-L3 knockdown significantly inhibited the proliferation of NSCLC cells, whereas UCH-L3 overexpression had the opposite effect."
32180254
reach
"UCHL3 promotes proliferation of colorectal cancer cells by regulating SOX12 via AKT and mTOR signaling pathway."
33194042
reach
"UCH-L3 promotes non small cell lung cancer proliferation via accelerating cell cycle and inhibiting cell apoptosis."
32180254
reach
"Moreover, UCH-L3 promoted NSCLC cells proliferation via accelerating cell cycle and inhibiting cell apoptosis."
32180254
UCHL3 inhibits cell population proliferation. 1 / 1
| 1
reach
"Genetic ablation of UCHL3 was found to markedly block ovarian cancer cell proliferation, viability and migration both in vitro and in vivo."
31477831
UCHL3 affects FOXM1
| 3 3
UCHL3 binds FOXM1.
| 1 3
UCHL3 binds FOXM1. 4 / 4
| 1 3
reach
"Co-immunoprecipitation (co-IP) and in vitro deubiquitination assays were performed to investigate the interactions between UCHL3 and FOXM1."
31598398
sparser
"For instance, the progression of pancreatic cancer is promoted by UCHL3-FOXM1 axis [ xref ]."
34016790
sparser
"Co-immunoprecipitation (co-IP) and in vitro deubiquitination assays were performed to investigate the interactions between UCHL3 and FOXM1."
31598398
sparser
"High UCHL3 expression was positively associated with FOXM1 expression level in pancreatic cancer patient samples."
31598398
UCHL3 deubiquitinates FOXM1.
| 2
UCHL3 deubiquitinates FOXM1. 2 / 2
| 2
reach
"Furthermore, knockdown of UCHL3 increased FOXM1 ubiquitination, which enhanced FOXM1 turnover and promoted pancreatic cancer cells ' sensitivity to gemcitabine."
31598398
reach
"Deubiquitination of FOXM1 by UCHL3 was also shown to promote pancreatic cancer progression and gemcitabine resistance."
33680951
UCHL3 affects BRD4
| 6
UCHL3 binds BRD4.
| 3
UCHL3 binds BRD4. 2 / 2
| 2
reach
"Of note, our experiments were performed in the presence of IL6/8. whether other deubiquitinases are preferential in other contexts need to be further investigated.To investigate if UCHL3 binds to BRD4, we performed reciprocal co-immunoprecipitation assays in cells transfected with BRD4, alone or with UCHL3 and JAK2-V617F."
34290255
reach
"Further investigation also showed the endogenous BRD4 binding with UCHL3 which was enhanced by rIL6 but inhibited by pacritinib (Figs."
34290255
UCHL3 binds phosphorylated BRD4. 1 / 1
| 1
reach
"This indicates that UCHL3 preferentially binds to a phosphorylated BRD4."
34290255
UCHL3 inhibits BRD4.
| 1
UCHL3 inhibits BRD4. 1 / 1
| 1
reach
"We thus propose that UCHL3 may serve as an additional target to block BRD4 signaling or re-sensitize CRC response to BETi, including BET-PROTACs, which warrant a further investigation."
34290255
UCHL3 deubiquitinates BRD4.
| 1
UCHL3 leads to the deubiquitination of BRD4. 1 / 1
| 1
reach
"Consistently, ubiquitination assay showed knockdown of UCHL3 promoted the poly-ubiquitination of BRD4 (Fig. 3e)."
34290255
UCHL3 activates BRD4.
| 1
UCHL3 activates BRD4. 1 / 1
| 1
reach
"Conversely, ectopic expression of UCHL3 prolonged the half-life of BRD4 (Fig. S4j); It also increased the protein stability of WT BRD4 but not BRD4-Y97/98A mutant (Fig. 3f)."
34290255
RAD51 affects UCHL3
4 | 1 1
UCHL3 binds RAD51. 6 / 6
4 | 1 1
biogrid
No evidence text available
27941124
sparser
"UCHL3 interacts with RAD51 and de-ubiquitinates residues that disrupt the RAD51–BRCA2 interaction."
31769469
biogrid
No evidence text available
27941124
reach
"Consist with this notion, it is suggested that UCHL3 interacts and deubiquitylates RAD51, which is required for the efficient HR [104]."
28764946
biogrid
No evidence text available
27941124
biogrid
No evidence text available
27941124
XRCC5 affects UCHL3
| 2 3
UCHL3 binds XRCC5. 5 / 5
| 2 3
sparser
"Accordingly, the interaction of Ku80 and UCHL3, which requires UCHL3 catalytic activity (Fig.  xref ), was not markedly affected when FLAG-tagged phospho-mutants of UCHL3 were examined by immunoprecipitation (Supplementary Fig.  xref )."
30559450
reach
"Accordingly, the interaction of Ku80 and UCHL3, which requires UCHL3 catalytic activity (Fig. 1E), was not markedly affected when FLAG-tagged phospho-mutants of UCHL3 were examined by immunoprecipitation (Supplementary Fig. S7B)."
30559450
reach
"Here we found that UCHL3, a deubiquitylating enzyme, interacts with and can directly deubiquitylate Ku80 in a catalytic activity dependent manner."
30559450
sparser
"UCHL3 interacts with Ku80 and is recruited to DNA damage sites in a manner dependent on its catalytic activity."
30559450
sparser
"Given the findings that UCHL3 interacted with Ku80 and was recruited to sites of damage in a catalytic activity dependent manner (Fig.  xref , Supplementary Fig.  xref ), we investigated whether catalytic activity is required for its phosphorylation."
30559450
USP5 affects UCHL3
1 | 1 3
UCHL3 binds USP5. 3 / 3
1 | 1 1
biogrid
No evidence text available
22939629
reach
"Thus, the binding of USP5 and UCHL3 to OTULIN ABP is largely non covalent and reversible."
28919039
sparser
"His-PD showed that OTULIN ABPΔG76 still bound to USP5 and UCHL3 ( xref I), confirming our MS results that these DUBs are associating largely in a non-covalent manner with the probes (compare xref B and 6C)."
28919039
SHBG binds UCHL3 and USP5. 2 / 2
| 2
sparser
"These results suggest that despite the partial labeling of USP5 in cell extracts, USP5 and UCHL3 are primarily bound to the OTULIN ABP in a non-covalent and reversible manner."
28919039
sparser
"Thus, the binding of USP5 and UCHL3 to OTULIN ABP is largely non-covalent and reversible."
28919039
UCHL3 affects KITLG
| 4 1
UCHL3 activates KITLG.
| 4
UCHL3 activates KITLG. 4 / 4
| 4
reach
"We further demonstrate that UCHL3 upregulates the levels of SCF beta-TrCP substrates including IFN-I receptor IFNAR1, which enhances IFN-I mediated signaling pathway and antiviral activity."
28583475
reach
"Thus, our findings identify a novel in vivo substrate of UCHL3, and reveal a novel deneddylation regulation by UCHL3.It is interesting that UCHL3 controls the activity of cellular SCF beta and TrCP co[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28583475
reach
"Furthermore, we reveal that UCHL3 increases levels of SCF beta-TrCP substrates and enhances IFN-I mediated signaling and antiviral activity."
28583475
reach
"Moreover, we find that UCHL3 upregulates levels of SCF beta-TrCP substrates including IFN-I receptor IFNAR1, thereby enhancing IFN-I mediated signaling and antiviral activity."
28583475
UCHL3 binds KITLG.
| 1
BTRC binds UCHL3, COPS5, CUL1, and KITLG. 1 / 1
| 1
sparser
"Interestingly, we find that UCHL3 regulates COPS5-dependent deneddylation of Cullin1, which is an essential component of SCF β-TrCP complex and associated with SCF β-TrCP activities."
28583475
UCHL3 affects CRY2
| 5
UCHL3 methylates CRY2.
| 4
UCHL3 leads to the methylation of CRY2. 4 / 4
| 4
reach
"In addition, UCHL3 knockdown decreased CRY2 methylation through downregulating FOXM1, leading to an increase in the expression of CRY2."
35088238
reach
"Knockdown of UCHL3 inhibits esophageal squamous cell carcinoma progression by reducing CRY2 methylation."
35088238
reach
"In summary, this study reveals that knockdown of UCHL3 inhibits ESCC growth and migration by reducing CRY2 methylation through downregulation of FOXM1 expression."
35088238
reach
"In the current study, we aimed to investigate the role of UCHL3 in ESCC growth and migration, and whether UCHL3 could modulate CRY2 methylation through FOXM1."
35088238
UCHL3 increases the amount of CRY2.
| 1
UCHL3 increases the amount of CRY2. 1 / 1
| 1
reach
"UCHL3 silencing inhibited cell viability, invasion, migration and induced cell apoptosis in vitro, repressed tumor growth in vivo, and increased CRY2 expression and decreased FOXM1 expression."
35088238
UCHL3 affects cell growth
| 4
UCHL3 activates cell growth.
| 3
UCHL3 activates cell growth. 3 / 3
| 3
reach
"UCHL3 overexpression enhanced A549 and H1299 cell growth in vitro (Fig. 2a, b)."
32546741
reach
"Moreover, CRY2 silencing abolished UCHL3 deficiency-mediated inhibition in cell growth and migration."
35088238
reach
"Our findings showed that knockdown of UCHL3 inhibited cell growth, migration and invasion in cervical cancer cells while UCHL3 knockdown inhibited cervical cancer development and metastasis in vivo in mouse models."
36527747
UCHL3 inhibits cell growth.
| 1
Mutated UCHL3 inhibits cell growth. 1 / 1
| 1
reach
"Interestingly, stable overexpression of UCHL3 mutants in A549 cells significantly attenuated cell growth compared to that in cells expressing WT UCHL3 (Fig. 6b)."
32546741
UCHL3 affects SP
| 4
SP binds UCHL3. 4 / 4
| 4
sparser
"Therefore, the expression patterns of Sp-UCHL3 and Sp-UCHL5 in different tissues and ovarian development suggest that they play important roles in cellular proteins’ degradation during oogenesis and ovarian maturation."
29351241
sparser
"Phylogenetic and Homology Analyses of Sp-UCHL3 and Sp-UCHL5."
29351241
sparser
"In the present study, Sp-UCHL3 mRNA expression in T3 was significantly higher than in the T1 and T2 stages ( p < 0.05), while Sp-UCHL5 mRNA expressions in the three stages of testes development were not significantly different from each other ( p > 0.05), indicating that Sp- UCHL3 and Sp- UCHL5 have different aspects in regulating mechanism of testis development."
29351241
sparser
"Prediction of Sp-UCHL3 and Sp-UCHL5 Three-Dimensional (3D) Structure."
29351241
UCHL3 affects NFE2L2
| 3 1
UCHL3 activates NFE2L2.
| 2
UCHL3 activates NFE2L2. 2 / 2
| 2
reach
"UCHL3 promotes cervical cancer development and metastasis by stabilizing NRF2 via deubiquitination."
36527747
reach
"Collectively, our findings suggest that UCHL3 promotes cervical cancer development and metastasis by stabilizing NRF2 via deubiquitination."
36527747
UCHL3 increases the amount of NFE2L2.
| 1
UCHL3 increases the amount of NFE2L2. 1 / 1
| 1
reach
"Knockdown of UCHL3 decreased NRF2 expression while overexpression of UCHL3 stabilized NRF2 via deubiquitination."
36527747
UCHL3 binds NFE2L2.
| 1
UCHL3 binds NFE2L2. 1 / 1
| 1
sparser
"Mechanistically, co-immunoprecipitation assay showed that UCHL3 directly interacted with NRF2."
36527747
UCHL3 affects Ku80 chromatin binding
| 4
UCHL3 activates Ku80 chromatin binding. 4 / 4
| 4
eidos
"UCHL3 enhances Ku80 chromatin binding and directly deubiquitylates Ku80 To examine the effect of UCHL3 on Ku regulation , we investigated IR-induced foci ( IRIF ) formation of Ku80 , which indicates loading of Ku onto DSB sites ."
30559450
eidos
"To gain insight into how UCHL3 contributes to Ku80 chromatin binding after DSB induction , firstly , we examined the effect of UCHL3 on the DNA-binding activity of Ku by electrophoretic mobility shift assay with purified these proteins ( Fig. 5A-C ) ."
30559450
eidos
"Since it was reported that Ku ubiquitylation is a prerequisite for Ku removal from chromatin , we hypothesized that UCHL3 may enhance Ku80 chromatin binding by antagonizing Ku80 ubiquitylation ."
30559450
eidos
"UCHL3 enhances Ku80 chromatin binding upon DSB induction ."
30559450
UCHL3 affects IFNAR1
| 4
UCHL3 increases the amount of IFNAR1.
| 3
UCHL3 increases the amount of IFNAR1. 2 / 2
| 2
reach
"These findings suggest that UCHL3 could positively regulate IFNAR1 levels via COPS5 mediated deneddylation of Cullin1, which is consistent with our previous findings that UCHL3 positively regulates IF[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28583475
reach
"In summary, our results demonstrate that UCHL3 upregulates the levels of SCF beta-TrCP substrates.We have demonstrated that UCHL3 positively regulated COPS5 levels and IFNAR1 levels."
28583475
Modified UCHL3 increases the amount of IFNAR1. 1 / 1
| 1
reach
"The result showed that UCHL3 overexpression gradually increased IFNAR1 levels."
28583475
UCHL3 activates IFNAR1.
| 1
UCHL3 activates IFNAR1. 1 / 1
| 1
reach
"This finding suggests that UCHL3 could target either TYK2 itself or IFN-I receptors (IFNAR1 and IFNAR2), which are the direct proteins upstream of TYK2 as well as the first signaling proteins in IFN-I[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28583475
UCHL3 affects HELLS
| 4
UCHL3 deubiquitinates HELLS.
| 2
UCHL3 deubiquitinates HELLS. 2 / 2
| 2
reach
"UCHL3 deubiquitinates chromatin modifier lymphoid-specific helicase (LSH)."
34405018
reach
"UCHL3 deubiquitinates chromatin modifier lymphoid-specific helicase (LSH)."
34405018
UCHL3 binds HELLS.
| 1
UCHL3 binds HELLS. 1 / 1
| 1
reach
"At the same time, the lncRNA GIAT4RA counteracts the deubiquitinating effects of LSH by interfering with interaction between LSH and UCH-L3, reducing levels of LSH protein, and playing the role in inhibiting the invasion and migration of LUAD cells83."
34405018
UCHL3 activates HELLS.
| 1
UCHL3 activates HELLS. 1 / 1
| 1
reach
"GIAT4RA interfered with ubiquitin hydrolase Uchl3 mediated interaction and stabilization of LSH."
31417184
TDP1 affects UCHL3
| 1 3
UCHL3 binds TDP1. 4 / 4
| 1 3
reach
"Furthermore, incubation of purified K48 ubiquitylated TDP1 with recombinant UCHL3 led to reduction of TDP1 ubiquitylation (XREF_FIG B) and a physical interaction between TDP1 and UCHL3 was observed by co-immunoprecipitation (XREF_FIG C)."
29898404
sparser
"Furthermore, incubation of purified K48-ubiquitylated TDP1 with recombinant UCHL3 led to reduction of TDP1 ubiquitylation ( xref B) and a physical interaction between TDP1 and UCHL3 was observed by co-immunoprecipitation ( xref C)."
29898404
sparser
"Here, we report multiple evidence showing a direct role of UCHL3 in controlling TDP1 proteostasis: (1) reduction of K48-ubiquitylated TDP1 in cancer cells overexpressing UCHL3, (2) reduction of K48-uibiquitylated TDP1 upon addition of recombinant UCHL3, (3) enrichment of K48-ubiquitylated TDP1 upon MG132 treatment, which is dependent on UCHL3 levels, (4) epistatic relationship showing that co-depletion of TDP1 and UCHL3 does not further sensitize cells to TOP1 poisons than depletion of either enzyme alone, (5) physical interaction between TDP1 and UCHL3, and (6) changes in the ubiquitylation status and TDP1 turnover upon changes in UCHL3 levels induced by its ectopic overexpression, siRNA depletion, CRISPR editing, and pharmacologic inhibition and, also, in two physiologically relevant clinical settings."
29898404
sparser
"We show that R586 methylation promotes TDP1 ubiquitylation, which facilitates ubiquitin/proteasome-dependent TDP1 turnover by impeding the binding of UCHL3 (deubiquitylase enzyme) with TDP1."
35705029
SP affects UCHL3
| 4
SP binds UCHL3. 4 / 4
| 4
sparser
"Therefore, the expression patterns of Sp-UCHL3 and Sp-UCHL5 in different tissues and ovarian development suggest that they play important roles in cellular proteins’ degradation during oogenesis and ovarian maturation."
29351241
sparser
"Phylogenetic and Homology Analyses of Sp-UCHL3 and Sp-UCHL5."
29351241
sparser
"In the present study, Sp-UCHL3 mRNA expression in T3 was significantly higher than in the T1 and T2 stages ( p < 0.05), while Sp-UCHL5 mRNA expressions in the three stages of testes development were not significantly different from each other ( p > 0.05), indicating that Sp- UCHL3 and Sp- UCHL5 have different aspects in regulating mechanism of testis development."
29351241
sparser
"Prediction of Sp-UCHL3 and Sp-UCHL5 Three-Dimensional (3D) Structure."
29351241
SLC4A1 affects UCHL3
| 4
SLC4A1 binds UCHL3.
| 3
SLC4A1 binds UCHL3. 3 / 3
| 3
reach
"Thus, we can not exclude the possibility that, under some conditions, UCH-L3 may interact with di-Ub to protect it from degradation.Previous biochemical analysis indicated that UCH-L3 is unable to hyd[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19154770
reach
"Likewise, UCH-L3 might interact with di-Ub to protect it from degradation."
19154770
reach
"To confirm the direct interaction of di-Ub and UCH-L3, we performed pull-down assays using recombinant mono-Ub or di-Ub and UCH-L3."
19154770
SLC4A1 inhibits UCHL3.
| 1
SLC4A1 inhibits UCHL3. 1 / 1
| 1
reach
"Thus, we speculated that di-Ub might inhibit the enzymatic activity of UCH-L3."
19154770
FOXM1 affects UCHL3
| 1 3
UCHL3 binds FOXM1. 4 / 4
| 1 3
reach
"Co-immunoprecipitation (co-IP) and in vitro deubiquitination assays were performed to investigate the interactions between UCHL3 and FOXM1."
31598398
sparser
"For instance, the progression of pancreatic cancer is promoted by UCHL3-FOXM1 axis [ xref ]."
34016790
sparser
"Co-immunoprecipitation (co-IP) and in vitro deubiquitination assays were performed to investigate the interactions between UCHL3 and FOXM1."
31598398
sparser
"High UCHL3 expression was positively associated with FOXM1 expression level in pancreatic cancer patient samples."
31598398
Trichostatin A affects UCHL3
3 |
Trichostatin A increases the amount of UCHL3. 3 / 3
3 |
ctd
No evidence text available
26272509
ctd
No evidence text available
27188386
ctd
No evidence text available
24935251
Cobalt dichloride affects UCHL3
3 |
Cobalt dichloride decreases the amount of UCHL3. 3 / 3
3 |
ctd
No evidence text available
19320972
ctd
No evidence text available
16622835
ctd
No evidence text available
19376846
Acetaldehyde affects UCHL3
| 3
Acetaldehyde inhibits UCHL3.
| 1
Acetaldehyde inhibits UCHL3. 1 / 1
| 1
reach
"The present data demonstrated that ubiquitin aldehyde can inhibit the activity of UCHL3."
27780264
Acetaldehyde increases the amount of UCHL3.
| 1
Acetaldehyde increases the amount of UCHL3. 1 / 1
| 1
reach
"Therefore, ubiquitin aldehyde may modulate the protein expression and function of UCHL3."
27780264
Acetaldehyde binds UCHL3.
| 1
Acetaldehyde binds UCHL3. 1 / 1
| 1
reach
"Binding of ubiquitin leads to apparent stabilization of the crossover loop, as it was seen straddling the active site in co-crystal structures of ubiquitin aldehyde bound to UCHL3 (XREF_FIG and XREF_FIG) and the yeast UCH Yuh1 XREF_BIBR, XREF_BIBR."
27012468
Ubiquitin affects UCHL1
| 3
Ubiquitin binds UCHL1 and UCHL3. 3 / 3
| 3
sparser
"T218, R227, F228, and L230 residues of BAP1N were selected based on ubiquitin interaction with the corresponding residues of UCHL1 and UCHL3."
28935764
sparser
"In contrast with BAP1, the thermodynamics of ubiquitin binding to UCHL1 and UCHL3 is dominated by enthalpy factors and hydrogen bonding and ionic interactions that stabilize the complex."
28935764
sparser
"Unlike UCH-L1, UCH-L3 can interact with Lys48-linked Ub dimers (di-Ub) to protect it from degradation and meanwhile to inhibit its hydrolase activity."
20302916
UCHL3 affects cell migration
| 3
UCHL3 activates cell migration.
| 2
UCHL3 activates cell migration. 2 / 2
| 2
reach
"Vimentin attenuated the suppression of cell migration caused by knockdown of UCHL3."
36969045
reach
"UCHL3 promotes hepatocellular carcinoma cell migration by de-ubiquitinating and stabilizing Vimentin."
36969045
UCHL3 inhibits cell migration.
| 1
reach
"In highly metastatic prostate PC3 cells, UCH-L3 inhibits the cell migration and invasion but UCH-L3 C95S had no effects, suggesting that the hydrolase activity of UCH-L3 is required to inhibit the met[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25194810
UCHL3 affects VIM
| 3
UCHL3 ubiquitinates VIM.
| 1
UCHL3 leads to the ubiquitination of VIM. 1 / 1
| 1
reach
"Moreover, the suppression of UCHL3 by siRNA or the inhibition by TCID upregulated ubiquitinated Vimentin."
36969045
UCHL3 deubiquitinates VIM.
| 1
UCHL3 leads to the deubiquitination of VIM. 1 / 1
| 1
reach
"Vimentin was identified as a potential de-ubiquitination substrate of UCHL3 and UCHL3 interacted with and promoted the de-ubiquitination of Vimentin, enhancing its stability."
36969045
UCHL3 binds VIM.
| 1
UCHL3 binds VIM. 1 / 1
| 1
reach
"co-immunoprecipitation, Immunofluorescence, and IHC were used to detect the interaction and expression association between UCHL3 and Vimentin in the cells."
36969045
UCHL3 affects TRAF2
| 3
UCHL3 deubiquitinates TRAF2.
| 2
UCHL3 leads to the deubiquitination of TRAF2. 2 / 2
| 2
reach
"The elevated UCHL3 facilitates carcinogenesis and enhances inflammation by deubiquitinating and stabilizing TNF Receptor Associated Factor 2 (TRAF2)."
33585209
reach
"UCHL3 was found to activate NF-kappaB signalling by deubiquitinating and stabilizing TRAF2, leading to tumourigenesis."
31477831
UCHL3 activates TRAF2.
| 1
UCHL3 activates TRAF2. 1 / 1
| 1
reach
"UCHL3 promotes ovarian cancer progression by stabilizing TRAF2 to activate the NF-kappaB pathway."
31477831
UCHL3 affects SLC4A1
| 3
SLC4A1 binds UCHL3. 3 / 3
| 3
reach
"Thus, we can not exclude the possibility that, under some conditions, UCH-L3 may interact with di-Ub to protect it from degradation.Previous biochemical analysis indicated that UCH-L3 is unable to hyd[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19154770
reach
"Likewise, UCH-L3 might interact with di-Ub to protect it from degradation."
19154770
reach
"To confirm the direct interaction of di-Ub and UCH-L3, we performed pull-down assays using recombinant mono-Ub or di-Ub and UCH-L3."
19154770
UCHL3 affects SHBG
| 3
SHBG binds UCHL3 and USP5. 2 / 2
| 2
sparser
"These results suggest that despite the partial labeling of USP5 in cell extracts, USP5 and UCHL3 are primarily bound to the OTULIN ABP in a non-covalent and reversible manner."
28919039
sparser
"Thus, the binding of USP5 and UCHL3 to OTULIN ABP is largely non-covalent and reversible."
28919039
SHBG binds UCHL3. 1 / 1
| 1
sparser
"The non-covalent binding of OTULIN ABP to UCHL3 is most likely due to the high abundance of this enzyme."
28919039
UCHL3 affects OTULIN ABP
| 3
UCHL3 binds OTULIN ABP. 3 / 3
| 3
reach
"The non covalent binding of OTULIN ABP to UCHL3 is most likely due to the high abundance of this enzyme."
28919039
reach
"These results suggest that despite the partial labeling of USP5 in cell extracts, USP5 and UCHL3 are primarily bound to the OTULIN ABP in a non covalent and reversible manner."
28919039
reach
"Thus, the binding of USP5 and UCHL3 to OTULIN ABP is largely non covalent and reversible."
28919039
reach
"In highly metastatic prostate PC3 cells, UCH-L3 inhibits the cell migration and invasion but UCH-L3 C95S had no effects, suggesting that the hydrolase activity of UCH-L3 is required to inhibit the met[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25194810
reach
"The invasion through the matrigel was increased by UCH-L3 knockdown."
25194810
UCHL3 activates Neoplasm Invasiveness.
| 1
UCHL3 activates Neoplasm Invasiveness. 1 / 1
| 1
reach
"Our findings showed that knockdown of UCHL3 inhibited cell growth, migration and invasion in cervical cancer cells while UCHL3 knockdown inhibited cervical cancer development and metastasis in vivo in mouse models."
36527747
UCHL3 affects Ku
| 1 2
UCHL3 binds Ku.
| 2
UCHL3 binds PRKDC and Ku. 1 / 1
| 1
sparser
"These data thus suggested that UCHL3 interacts with Ku and/or DNA-PKcs in cells and could have a role at sites of DSBs in a catalytic-activity-dependent manner."
30559450
UCHL3 binds Ku. 1 / 1
| 1
sparser
"The immunoprecipitation of endogenous UCHL3 using an anti-UCHL3 antibody followed by immunoblotting analysis detected the interaction with endogenous Ku80, providing further support that UCHL3 interacts with Ku (Fig.  xref )."
30559450
UCHL3 ubiquitinates Ku.
| 1
UCHL3 leads to the ubiquitination of Ku. 1 / 1
| 1
reach
"Lastly, we provide evidence showing that DNA damage-induced UCHL3 phosphorylation (which is dependent on UCHL3 catalytic activity, ATM kinase and downstream NHEJ factors) promotes destabilization of UCHL3, suggesting that UCHL3 may dissociate from Ku80 to allow Ku ubiquitylation and subsequent removal from DNA."
30559450
UCHL3 affects INS
| 3
UCHL3 activates INS.
| 2
UCHL3 activates INS. 2 / 2
| 2
reach
"Suzuki et al. have shown that UCHL3 enhances insulin signalling in (pre) adipocytes, but UCHL3 is unlikely to target critical components of insulin signalling like the insulin receptor, the IGF-I receptor, IRS-1 and Grb10 XREF_BIBR."
22679485
reach
"These results suggest that UCH-L3 promotes adipogenesis by enhancing insulin signaling in a hydrolase activity dependent manner."
19837878
UCHL3 phosphorylates INS.
| 1
Modified UCHL3 leads to the phosphorylation of INS. 1 / 1
| 1
reach
"Moreover, ectopic expression of wild-type UCH-L3 restored the phosphorylation of insulin and IGF-I receptor and adipocyte differentiation in Uchl3 (-/-) MEFs."
19837878
UCHL3 affects FlaG
| 3
UCHL3 binds FlaG. 2 / 2
| 2
sparser
"We observed that FLAG-BAP1 ( xref B, upper panel), but not FLAG-UCHL3 ( xref B, lower panel), directly binds to HA-KEAP1."
35052618
sparser
"These data were in line with observed c-NHEJ activities being reduced in UCHL3 KO#2 cells, which was largely rescued by exogenously expressed FLAG-UCHL3 (Fig.  xref )."
30559450
UCHL3 binds BAP1 and FlaG. 1 / 1
| 1
sparser
"We observed that FLAG-BAP1 ( xref B, upper panel), but not FLAG-UCHL3 ( xref B, lower panel), directly binds to HA-KEAP1."
35052618
UCHL3 affects CTNNB1
| 3
UCHL3 increases the amount of CTNNB1.
| 2
UCHL3 increases the amount of CTNNB1. 1 / 1
| 1
reach
"Similarly, knockdown of UCHL3 decreased the expression level of Myc-beta-catenin, and overexpression of UCHL3 upregulated Myc-beta-catenin levels in a dose dependent manner."
28583475
Modified UCHL3 increases the amount of CTNNB1. 1 / 1
| 1
reach
"Similarly, knockdown of UCHL3 decreased the expression level of Myc-beta-catenin, and overexpression of UCHL3 upregulated Myc-beta-catenin levels in a dose dependent manner."
28583475
UCHL3 decreases the amount of CTNNB1.
| 1
Modified UCHL3 decreases the amount of CTNNB1. 1 / 1
| 1
reach
"Similarly, knockdown of UCHL3 decreased the expression level of Myc-beta-catenin, and overexpression of UCHL3 upregulated Myc-beta-catenin levels in a dose dependent manner."
28583475
UCHL3 affects CLPB
3 |
UCHL3 binds CLPB. 3 / 3
3 |
biogrid
No evidence text available
28514442
biogrid
No evidence text available
19615732
biogrid
No evidence text available
26186194
UCHL3 affects ABCG2
| 3
UCHL3 increases the amount of ABCG2.
| 2
UCHL3 increases the amount of ABCG2. 2 / 2
| 2
reach
"As expected, overexpression of UCHL3 increased the expression of ABCG2, c-Myc and KLF4, genes associated with stemness, at the protein level in A549 and H1299 cells (Fig. 5b, c), while depletion of UCHL3 decreased their protein expression (Fig. 5d)."
32546741
reach
"FACS analysis showed that inhibition of UCHL3 using TCID reduced CD338 expression and the percentage of SP cells among A549 cells overexpressing UCHL3 (Fig. 7e, f)."
32546741
UCHL3 activates ABCG2.
| 1
UCHL3 activates ABCG2. 1 / 1
| 1
reach
"Flow cytometry analysis indicated that knockdown of UCHL3 reduced the proportion of CD338 H358 cells (Fig. 3d)."
32546741
UCHL1 affects Ubiquitin
| 3
Ubiquitin binds UCHL1 and UCHL3. 3 / 3
| 3
sparser
"T218, R227, F228, and L230 residues of BAP1N were selected based on ubiquitin interaction with the corresponding residues of UCHL1 and UCHL3."
28935764
sparser
"In contrast with BAP1, the thermodynamics of ubiquitin binding to UCHL1 and UCHL3 is dominated by enthalpy factors and hydrogen bonding and ionic interactions that stabilize the complex."
28935764
sparser
"Unlike UCH-L1, UCH-L3 can interact with Lys48-linked Ub dimers (di-Ub) to protect it from degradation and meanwhile to inhibit its hydrolase activity."
20302916
UBD affects UCHL3
| 1 1 1
UBD inhibits UCHL3. 3 / 3
| 1 1 1
reach
"K27 Linked Diubiquitin Inhibits UCHL3 via an Unusual Kinetic Trap."
33238157
sparser
"K27-Linked Diubiquitin Inhibits UCHL3 via an Unusual Kinetic Trap."
33238157
trips
"K27-Linked Diubiquitin Inhibits UCHL3 via an Unusual Kinetic Trap."
33238157
SHBG affects UCHL3
| 3
SHBG binds UCHL3 and USP5. 2 / 2
| 2
sparser
"These results suggest that despite the partial labeling of USP5 in cell extracts, USP5 and UCHL3 are primarily bound to the OTULIN ABP in a non-covalent and reversible manner."
28919039
sparser
"Thus, the binding of USP5 and UCHL3 to OTULIN ABP is largely non-covalent and reversible."
28919039
SHBG binds UCHL3. 1 / 1
| 1
sparser
"The non-covalent binding of OTULIN ABP to UCHL3 is most likely due to the high abundance of this enzyme."
28919039
OTULIN ABP affects UCHL3
| 3
UCHL3 binds OTULIN ABP. 3 / 3
| 3
reach
"The non covalent binding of OTULIN ABP to UCHL3 is most likely due to the high abundance of this enzyme."
28919039
reach
"These results suggest that despite the partial labeling of USP5 in cell extracts, USP5 and UCHL3 are primarily bound to the OTULIN ABP in a non covalent and reversible manner."
28919039
reach
"Thus, the binding of USP5 and UCHL3 to OTULIN ABP is largely non covalent and reversible."
28919039
JUN affects UCHL3
3 |
JUN decreases the amount of UCHL3. 3 / 3
3 |
biopax:msigdb
No evidence text available
biopax:msigdb
No evidence text available
biopax:msigdb
No evidence text available
FlaG affects UCHL3
| 3
UCHL3 binds FlaG. 2 / 2
| 2
sparser
"We observed that FLAG-BAP1 ( xref B, upper panel), but not FLAG-UCHL3 ( xref B, lower panel), directly binds to HA-KEAP1."
35052618
sparser
"These data were in line with observed c-NHEJ activities being reduced in UCHL3 KO#2 cells, which was largely rescued by exogenously expressed FLAG-UCHL3 (Fig.  xref )."
30559450
UCHL3 binds BAP1 and FlaG. 1 / 1
| 1
sparser
"We observed that FLAG-BAP1 ( xref B, upper panel), but not FLAG-UCHL3 ( xref B, lower panel), directly binds to HA-KEAP1."
35052618
Delta12-PGJ2 affects UCHL3
| 3
Delta12-PGJ2 inhibits UCHL3. 3 / 3
| 3
reach
"As shown in Fig. 5, Delta12-PGJ2 inhibited the activities of UCH-L1 (K i = 3.49 muM +/- 0.42, Fig. 5A) and UCH-L3 (K i = 8.1 muM +/- 0.8, Fig. 5B), indicating that this PGJ2 metabolite is an efficient[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15194490
reach
"Since Delta12-PGJ2 inhibited UCH-L1 and UCH-L3 activities, we postulated that this cyclopentenone prostaglandin would affect the endogenous levels of ubiquitinated proteins."
15194490
reach
"Delta12-PGJ2 and 15Delta-PGJ2 are potent cyclopentone PGs and have been reported to inhibit UCH-L3 and UCH-L1 respectively [282,283]."
28923280
CLPB affects UCHL3
3 |
UCHL3 binds CLPB. 3 / 3
3 |
biogrid
No evidence text available
28514442
biogrid
No evidence text available
19615732
biogrid
No evidence text available
26186194
BRD4 affects UCHL3
| 3
UCHL3 binds BRD4. 2 / 2
| 2
reach
"Of note, our experiments were performed in the presence of IL6/8. whether other deubiquitinases are preferential in other contexts need to be further investigated.To investigate if UCHL3 binds to BRD4, we performed reciprocal co-immunoprecipitation assays in cells transfected with BRD4, alone or with UCHL3 and JAK2-V617F."
34290255
reach
"Further investigation also showed the endogenous BRD4 binding with UCHL3 which was enhanced by rIL6 but inhibited by pacritinib (Figs."
34290255
UCHL3 binds phosphorylated BRD4. 1 / 1
| 1
reach
"This indicates that UCHL3 preferentially binds to a phosphorylated BRD4."
34290255
AHR affects UCHL3
| 3
UCHL3 binds AHR. 3 / 3
| 3
sparser
"UCHL3 interacts with AhR and stabilizes the AhR protein through deubiquitination."
32546741
sparser
"Then, we explored the interaction between the UCHL3 and AhR proteins."
32546741
sparser
"First, UCHL3 and AhR directly interact."
32546741
Ubiquitin inhibitor affects UCHL3
| 2
UCHL3 binds ubiquitin inhibitor. 2 / 2
| 2
reach
"The high preference at the P3 position and relatively low specificity in the P4 position can be explained by comparison with the published crystal structure of UCH-L3 bound with ubiquitin inhibitor (XREF_FIG)."
18601651
reach
"The high preference at the P3 position and relatively low specificity in the P4 position can be explained by comparison with the published crystal structure of UCH-L3 bound with ubiquitin inhibitor (Fig.3) ."
18601651
Sodium citrate affects UCHL3
| 2
Sodium citrate activates UCHL3. 2 / 2
| 2
reach
"Sodium citrate, at high concentrations, activated OTU-1, UCH-L3 and PLpro, and this could be due to an ionic effect or a Hofmeister effect."
18601651
reach
"Sodium citrate at 0.8M enhanced activity of UCH-L3 by 12-fold versus 0.8M NaCl for Ac-LRGG-AFC and 20-fold for Ac-ALRGG-AFC (Fig. 5B )."
18601651
reach
"Sodium citrate, at high concentrations, activated OTU-1, UCH-L3 and PLpro, and this could be due to an ionic effect or a Hofmeister effect."
18601651
reach
"Sodium citrate at 0.8 M enhanced activity of UCH-L3 by 12-fold versus 0.8 M NaCl for Ac-LRGG-AFC and 20-fold for Ac-ALRGG-AFC (XREF_FIG)."
18601651
Sirolimus affects UCHL3
| 2
Sirolimus increases the amount of UCHL3.
| 1
Sirolimus increases the amount of UCHL3. 1 / 1
| 1
reach
"The aim of this study was to explore whether rapamycin can induce the autophagy of HL-60 cells and UCH-L3 expression."
30111405
Sirolimus activates UCHL3.
| 1
Sirolimus activates UCHL3. 1 / 1
| 1
reach
"[Autophagy and Expression of UCH-L3 Induced by Rapamycin in HL-60 Cells]."
30111405
Pirinixic acid affects UCHL3
2 |
Pirinixic acid increases the amount of UCHL3. 2 / 2
2 |
ctd
No evidence text available
23811191
ctd
No evidence text available
18301758
Phenylmercury acetate increases the amount of UCHL3. 2 / 2
2 |
ctd
No evidence text available
26272509
ctd
No evidence text available
27188386
Hsa-miR-875-5p affects UCHL3
2 |
Hsa-miR-875-5p decreases the amount of UCHL3. 2 / 2
2 |
biopax:mirtarbase
No evidence text available
21572407
biopax:mirtarbase
No evidence text available
23446348
Hsa-miR-8485 affects UCHL3
2 |
Hsa-miR-8485 decreases the amount of UCHL3. 2 / 2
2 |
biopax:mirtarbase
No evidence text available
21572407
biopax:mirtarbase
No evidence text available
23446348
Hsa-miR-603 affects UCHL3
2 |
Hsa-miR-603 decreases the amount of UCHL3. 2 / 2
2 |
biopax:mirtarbase
No evidence text available
23446348
biopax:mirtarbase
No evidence text available
21572407
Hsa-miR-5580-3p affects UCHL3
2 |
Hsa-miR-5580-3p decreases the amount of UCHL3. 2 / 2
2 |
biopax:mirtarbase
No evidence text available
23446348
biopax:mirtarbase
No evidence text available
21572407
Hsa-miR-5011-5p affects UCHL3
2 |
Hsa-miR-5011-5p decreases the amount of UCHL3. 2 / 2
2 |
biopax:mirtarbase
No evidence text available
23446348
biopax:mirtarbase
No evidence text available
21572407
Hsa-miR-4789-3p affects UCHL3
2 |
Hsa-miR-4789-3p decreases the amount of UCHL3. 2 / 2
2 |
biopax:mirtarbase
No evidence text available
21572407
biopax:mirtarbase
No evidence text available
23446348
Hsa-miR-3941 affects UCHL3
2 |
Hsa-miR-3941 decreases the amount of UCHL3. 2 / 2
2 |
biopax:mirtarbase
No evidence text available
23446348
biopax:mirtarbase
No evidence text available
21572407
Hsa-miR-362-3p affects UCHL3
2 |
Hsa-miR-362-3p decreases the amount of UCHL3. 2 / 2
2 |
biopax:mirtarbase
No evidence text available
21572407
biopax:mirtarbase
No evidence text available
23446348
Hsa-miR-329-3p affects UCHL3
2 |
Hsa-miR-329-3p decreases the amount of UCHL3. 2 / 2
2 |
biopax:mirtarbase
No evidence text available
23446348
biopax:mirtarbase
No evidence text available
21572407
Hsa-miR-3144-3p affects UCHL3
2 |
Hsa-miR-3144-3p decreases the amount of UCHL3. 2 / 2
2 |
biopax:mirtarbase
No evidence text available
23446348
biopax:mirtarbase
No evidence text available
21572407
Hsa-miR-190a-3p affects UCHL3
2 |
Hsa-miR-190a-3p decreases the amount of UCHL3. 2 / 2
2 |
biopax:mirtarbase
No evidence text available
21572407
biopax:mirtarbase
No evidence text available
23446348
Cyclosporin A affects UCHL3
2 |
Cyclosporin A increases the amount of UCHL3. 2 / 2
2 |
ctd
No evidence text available
20106945
ctd
No evidence text available
25562108
ZMYM6 affects UCHL3
2 |
UCHL3 binds ZMYM6. 2 / 2
2 |
biogrid
No evidence text available
28514442
biogrid
No evidence text available
26186194
ZEB1 affects UCHL3
2 |
ZEB1 binds UCHL3. 2 / 2
2 |
biogrid
No evidence text available
29119051
biogrid
No evidence text available
29119051
Ub dimers affects UCHL3
| 2
Ub dimers inhibits UCHL3.
| 1
Ub dimers inhibits UCHL3. 1 / 1
| 1
reach
"These different phenotypes of mutant mice indicate that although both enzymes share similar properties in terms of biochemical identity (Johnston et al., 1997; Larsen et al., 1998; Misaghi et al., 200[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19154770
Ub dimers binds UCHL3.
| 1
UCHL3 binds Ub dimers. 1 / 1
| 1
reach
"Here we show that Ub dimers interact non covalently with UCH-L3 in vitro and in cells."
19154770
USP5 affects SHBG
| 2
SHBG binds UCHL3 and USP5. 2 / 2
| 2
sparser
"These results suggest that despite the partial labeling of USP5 in cell extracts, USP5 and UCHL3 are primarily bound to the OTULIN ABP in a non-covalent and reversible manner."
28919039
sparser
"Thus, the binding of USP5 and UCHL3 to OTULIN ABP is largely non-covalent and reversible."
28919039
UCHL5 affects UCHL3
| 2
UCHL5 binds UCHL3.
| 1
UCHL3 binds UCHL5. 1 / 1
| 1
reach
"To delineate the structural basis of the auto-inhibition of Uch37 and its activation by Rpn13C, we used a Uch37 dimer model derived from its crystal structure and compared it with the structure of the binary complex of ubiquitin bound Uch37 (PDB ID 4IG7) and its homolog UCH-L3 (PDB ID 1XD3)."
24752541
UCHL5 activates UCHL3.
| 1
UCHL5 activates UCHL3. 1 / 1
| 1
reach
"Overexpression of UCH-L5 (but not overexpression of UCH-L3) for two days prior to the infection with Salmonella for one hour also lead to significant cell death (XREF_FIG), while cell treatment with b-AP15 inhibitor prior to Salmonella infection led to slight increase in cell viability in comparison to vehicle treated cells (XREF_SUPPLEMENTARY)."
26267804
UCHL3 affects ubiquitin inhibitor
| 2
UCHL3 binds ubiquitin inhibitor. 2 / 2
| 2
reach
"The high preference at the P3 position and relatively low specificity in the P4 position can be explained by comparison with the published crystal structure of UCH-L3 bound with ubiquitin inhibitor (XREF_FIG)."
18601651
reach
"The high preference at the P3 position and relatively low specificity in the P4 position can be explained by comparison with the published crystal structure of UCH-L3 bound with ubiquitin inhibitor (Fig.3) ."
18601651
UCHL3 affects radio-sensitivity cells
| 2
UCHL3 inhibits radio-sensitivity cells. 2 / 2
| 2
eidos
"Moreover , we found that knockdown of UCHL3 enhanced the radio-sensitivity of NSCLC cells both in vitro and in vivo ."
34016790
eidos
"Collectively , our study revealed that knockdown of UCHL3 enhanced the radio-sensitivity of NSCLC cells and increased IR-induced DNA damage via impairing HR repair ."
34016790
UCHL3 activates osteoblast differentiation. 2 / 2
| 2
eidos
"Importantly , UCH-L3 deubiquitylates Smad1 and enhances osteoblast differentiation [ 73 ] ."
30123285
eidos
"UCH-L3 activates osteoblast differentiation by deubiquitinating and stabilizing Smad1 ( Kim et al ., 2011 ) ."
36340031
eidos
"The elevated UCHL3 facilitates carcinogenesis and enhances inflammation by deubiquitinating and stabilizing TNF Receptor Associated Factor 2 ( TRAF2 ) ( 74 ) ."
33585209
reach
"Our results indicate that highly expressed UCHL3 enhances inflammation by stabilizing TRAF2, which in turn facilitates tumourigenesis in ovarian cancer, and that UCHL3 is a potential target for ovarian cancer patients with increased inflammation."
31477831
reach
"Furthermore, UCHL3 was recently suggested to facilitate HR by deubiquitylating RAD51 in a DSB-dependent manner via phosphorylation on the same site that we identified (S75) , however, we did not observe any defects in HR caused by UCHL3 depletion, nor did we detect any interaction with RAD51 in our mass spectrometry analyses."
30559450
reach
"Ablating UCHL3 significantly attenuates farrerol-mediated stimulation in HR and SCNT embryo development."
37012254
UCHL3 activates double-strand break repair via classical nonhomologous end joining. 2 / 2
| 1 1
eidos
"Together , these data support the idea that UCHL3 promotes c-NHEJ , not HR , in a catalytic activity-dependent manner ."
30559450
reach
"Together, these data support the idea that UCHL3 promotes c-NHEJ, not HR, in a catalytic activity-dependent manner."
30559450
UCHL3 affects ZMYM6
2 |
UCHL3 binds ZMYM6. 2 / 2
2 |
biogrid
No evidence text available
28514442
biogrid
No evidence text available
26186194
UCHL3 affects ZEB1
2 |
ZEB1 binds UCHL3. 2 / 2
2 |
biogrid
No evidence text available
29119051
biogrid
No evidence text available
29119051
UCHL3 affects YAP1
1 | 1
UCHL3 increases the amount of YAP1.
| 1
UCHL3 increases the amount of YAP1. 1 / 1
| 1
reach
"Depletion of UCHL3 decreased the YAP protein level and the expression of YAP/TEAD target genes in ATC."
36813921
UCHL3 binds YAP1.
1 |
UCHL3 binds YAP1. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects UCHL5
| 2
UCHL3 increases the amount of UCHL5.
| 1
UCHL3 increases the amount of UCHL5. 1 / 1
| 1
reach
"This indicates that UCH-L5 protein levels are modulated by either a pathogen or a host, and only the activity of UCH-L3 is changed during infection with Salmonella, which could be caused by changes in post-translational modifications of this enzyme [XREF_BIBR]."
26267804
UCHL3 binds UCHL5.
| 1
UCHL3 binds UCHL5. 1 / 1
| 1
reach
"To delineate the structural basis of the auto-inhibition of Uch37 and its activation by Rpn13C, we used a Uch37 dimer model derived from its crystal structure and compared it with the structure of the binary complex of ubiquitin bound Uch37 (PDB ID 4IG7) and its homolog UCH-L3 (PDB ID 1XD3)."
24752541
UCHL3 affects UCHL3
| 2
UCHL3 increases the amount of UCHL3.
| 1
UCHL3 increases the amount of UCHL3. 1 / 1
| 1
reach
"UCHL3 promoter analysis revealed that TEAD4, through which YAP bind to DNA, activated UCHL3 transcription by binding to the promoter of UCHL3."
36813921
UCHL3 activates UCHL3.
| 1
Modified UCHL3 activates UCHL3. 1 / 1
| 1
reach
"Moreover, ectopic expression of wild-type UCH-L3 restored the phosphorylation of insulin and IGF-I receptor and adipocyte differentiation in Uchl3 (-/-) MEFs."
19837878
UCHL3 affects UBA6
2 |
UCHL3 binds UBA6. 2 / 2
2 |
biogrid
No evidence text available
26344197
biogrid
No evidence text available
22939629
UCHL3 affects TGFB
| 2
UCHL3 activates TGFB. 2 / 2
| 2
reach
"Overexpression of UCH-L1, but not of UCH-L3 (the other human homolog of UBH1) or of the catalytic mutant UCH L1C90A, enhanced TGF-beta and SMAD-induced transcriptional activity, indicating that the deubiquitination activity of UCH-L1 is indispensable for enhancing TGF-beta and SMAD signaling."
32371398
reach
"Overexpression of UCH-L1, but not of UCH-L3 (the other human homolog of UBH1) or of the catalytic mutant UCH-L1 C90A, enhanced TGF-beta and SMAD-induced transcriptional activity, indicating that the deubiquitination activity of UCH-L1 is indispensable for enhancing TGF-beta and SMAD signaling."
33516505
UCHL3 affects SLC11A1
| 1 1
SLC11A1 binds UCHL3. 2 / 2
| 1 1
reach
"However, HOTAIR was also reported to reduce the ubiquitination degradation of AR by preventing its combination with MDM2, and GIAT4RA was recently reported to interfere the interaction between Uchl3 and LSH to repress ubiquitin-proteasome pathway [30], which indicate multiple roles of lncRNA in ubiquitination."
34759344
sparser
"However, HOTAIR was also reported to reduce the ubiquitination degradation of AR by preventing its combination with MDM2, and GIAT4RA was recently reported to interfere the interaction between Uchl3 and LSH to repress ubiquitin-proteasome pathway [ xref ], which indicate multiple roles of lncRNA in ubiquitination."
34759344
UCHL3 inhibits Radiation, Ionizing. 2 / 2
| 2
reach
"In addition, since the increased sensitivity to IR caused by UCHL3 depletion was moderate and the human genome encodes around 100 DUBs, it would be interesting to examine if any other DUBs can deubiquitylate Ku."
30559450
reach
"To exclude the possibility that the increased IR sensitivity caused by UCHL3 depletion was due to decreased proliferation or cell cycle issues, cell proliferation and cell cycle profile of these cell lines were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-based assay and bromodeoxyuridine (BrdU) incorporation assay, respectively (Supplementary Fig. S2A,B)."
30559450
UCHL3 affects PRKDC
| 2
UCHL3 binds PRKDC. 1 / 1
| 1
sparser
"Our mass spectrometry and ensuing immunoprecipitation analysis indicated that UCHL3 could interact with DNA-PKcs, possibly suggesting context specific functions of UCHL3 for maintaining genome integrity."
30559450
UCHL3 binds PRKDC and Ku. 1 / 1
| 1
sparser
"These data thus suggested that UCHL3 interacts with Ku and/or DNA-PKcs in cells and could have a role at sites of DSBs in a catalytic-activity-dependent manner."
30559450
UCHL3 affects PPARG
| 2
UCHL3 deubiquitinates PPARG.
| 1
UCHL3 deubiquitinates PPARG. 1 / 1
| 1
reach
"In addition, recombinant UCHL3 failed to deubiquitinate PPARgamma in vitro (XREF_SUPPLEMENTARY and XREF_SUPPLEMENTARY), indicating that UCHL3 modulates PPARgamma activity by an indirect mechanism, either through its ubiquitin hydrolase or its deneddylase activity."
22679485
UCHL3 activates PPARG.
| 1
UCHL3 activates PPARG. 1 / 1
| 1
reach
"In addition, recombinant UCHL3 failed to deubiquitinate PPARgamma in vitro (XREF_SUPPLEMENTARY and XREF_SUPPLEMENTARY), indicating that UCHL3 modulates PPARgamma activity by an indirect mechanism, either through its ubiquitin hydrolase or its deneddylase activity."
22679485
UCHL3 inhibits Neoplastic Stem Cells. 2 / 2
| 2
reach
"The CSC-like properties were enhanced by UCH-L1 but inhibited by UCH-L3."
34745437
reach
"In DU145 cells, the CSC-like properties were inhibited by UCH-L1 knockdown or UCH-L3 overexpression."
34745437
UCHL3 affects MYC
| 2
UCHL3 increases the amount of MYC. 2 / 2
| 2
reach
"WT UCHL3 obviously increased AhR and c-Myc protein levels, whereas the mutants did not (Fig. 6a)."
32546741
reach
"As expected, overexpression of UCHL3 increased the expression of ABCG2, c-Myc and KLF4, genes associated with stemness, at the protein level in A549 and H1299 cells (Fig. 5b, c), while depletion of UCHL3 decreased their protein expression (Fig. 5d)."
32546741
UCHL3 affects LMO7
| 1 1
UCHL3 binds LMO7. 1 / 1
| 1
reach
"We suggest that the increase in severity may reflect an interaction between Uchl3 and Lmo7 in the ubiquitin mediated protein degradation pathway."
12761045
Ubiquitin binds UCHL3 and LMO7. 1 / 1
| 1
sparser
"We suggest that the increase in severity may reflect an interaction between Uchl3 and Lmo7 in the ubiquitin-mediated protein degradation pathway."
12761045
UCHL3 affects GARS1
| 2
UCHL3 deubiquitinates GARS1.
| 1
UCHL3 deubiquitinates GARS1. 1 / 1
| 1
reach
"Importantly, UCH-L3 deubiquitylates Smad1 and enhances osteoblast differentiation [73]."
30123285
UCHL3 activates GARS1.
| 1
UCHL3 activates GARS1. 1 / 1
| 1
reach
"This study suggests that mechanical force-induced macrophage-derived exosomal UCHL3 promotes BMSCs osteogenesis by targeting SMAD1, thereby promoting alveolar bone formation during OTM."
36915132
UCHL3 affects DNA repair
| 2
UCHL3 inhibits DNA repair.
| 1
UCHL3 inhibits DNA repair. 1 / 1
| 1
reach
"In addition, siRNA-mediated depletion of UCHL3 causes a DNA repair defect that is largely restored upon complementation of cells with wild-type UCHL3, which correlates with depletion or genetic deletion of UCHL3 moderately sensitising cells to IR."
30559450
UCHL3 activates DNA repair.
| 1
UCHL3 activates DNA repair. 1 / 1
| 1
reach
"Silencing UCHL3 enhances radio-sensitivity of non small cell lung cancer cells by inhibiting DNA repair."
34016790
UCHL3 affects Cell Survival
| 1 1
UCHL3 activates Cell Survival. 2 / 2
| 1 1
reach
"UCHL3 promotes cell survival after DSB induction and is epistatic with XRCC4."
30559450
eidos
"UCHL3 promotes cell survival after DSB induction and is epistatic with XRCC4 To further explore whether UCHL3 plays a role in cellular responses to DSBs , clonogenic survival assays were performed upon treating cells with various short interfering RNAs ( siRNAs ) targeting UCHL3 ."
30559450
UCHL3 affects CRY2 methylation
| 2
UCHL3 activates CRY2 methylation. 2 / 2
| 2
eidos
"In the current study , we aimed to investigate the role of UCHL3 in ESCC growth and migration , and whether UCHL3 could modulate CRY2 methylation through FOXM1 ."
35088238
eidos
"In addition , UCHL3 knockdown decreased CRY2 methylation through downregulating FOXM1 , leading to an increase in the expression of CRY2 ."
35088238
UCHL3 affects BAP1
| 2
UCHL3 binds BAP1 and FlaG. 1 / 1
| 1
sparser
"We observed that FLAG-BAP1 ( xref B, upper panel), but not FLAG-UCHL3 ( xref B, lower panel), directly binds to HA-KEAP1."
35052618
UCHL3 binds KEAP1 and BAP1. 1 / 1
| 1
sparser
"To examine whether BAP1 and UCHL3 directly interacts with KEAP1, we cotransfected pcDNA3-FLAG-BAP1 or pcDNA3-FLAG-UCHL3 with pcDNA3-HA-KEAP1 in 293T cells and immunoprecipitated cell lysates with FLAG-agarose beads followed by Western blot analysis with HA-HRP antibody."
35052618
UCHL3 affects ATXN7L3
1 1 |
UCHL3 binds ATXN7L3. 2 / 2
1 1 |
biogrid
No evidence text available
16713569
hprd
No evidence text available
16713569
UCHL3 affects ATM
1 | 1
UCHL3 binds ATM.
1 |
UCHL3 binds ATM. 1 / 1
1 |
biogrid
No evidence text available
27941124
UCHL3 activates ATM.
| 1
UCHL3 activates ATM. 1 / 1
| 1
reach
"UCHL3 depletion significantly decreased ATC progression, stem-like and metastasis, and increased cell sensitivity to chemotherapy."
36813921
UBA6 affects UCHL3
2 |
UCHL3 binds UBA6. 2 / 2
2 |
biogrid
No evidence text available
26344197
biogrid
No evidence text available
22939629
TCID affects UCHL3
| 2
TCID inhibits UCHL3. 2 / 2
| 2
reach
"Moreover, TCID attenuated UCHL3 activity without affecting its protein level."
32546741
reach
"To further demonstrate our findings, we used TCID, a small-molecule inhibitor of UCHL3; under our experimental conditions, we first detected whether TCID inhibits the deubiquitinase activity of UCHL3 against AhR."
32546741
SLC11A1 affects UCHL3
| 1 1
SLC11A1 binds UCHL3. 2 / 2
| 1 1
reach
"However, HOTAIR was also reported to reduce the ubiquitination degradation of AR by preventing its combination with MDM2, and GIAT4RA was recently reported to interfere the interaction between Uchl3 and LSH to repress ubiquitin-proteasome pathway [30], which indicate multiple roles of lncRNA in ubiquitination."
34759344
sparser
"However, HOTAIR was also reported to reduce the ubiquitination degradation of AR by preventing its combination with MDM2, and GIAT4RA was recently reported to interfere the interaction between Uchl3 and LSH to repress ubiquitin-proteasome pathway [ xref ], which indicate multiple roles of lncRNA in ubiquitination."
34759344
SHBG affects USP5
| 2
SHBG binds UCHL3 and USP5. 2 / 2
| 2
sparser
"These results suggest that despite the partial labeling of USP5 in cell extracts, USP5 and UCHL3 are primarily bound to the OTULIN ABP in a non-covalent and reversible manner."
28919039
sparser
"Thus, the binding of USP5 and UCHL3 to OTULIN ABP is largely non-covalent and reversible."
28919039
RA-9 affects UCHL3
| 2
RA-9 inhibits UCHL3. 2 / 2
| 2
reach
"Indeed, it was found that RA-9 inhibited in vitro UCHL1, UCHL3, USP8 and USP9x, besides USP14 and UCHL5."
27381943
reach
"RA-9, a chalcone derivative with a structure similar to b-AP15, was reported to inhibit proteasomal DUBs [XREF_BIBR] as well as UCHL1, UCHL3, USP2, USP5, and USP8 [XREF_BIBR]."
29039082
PRKDC affects UCHL3
| 2
UCHL3 binds PRKDC. 1 / 1
| 1
sparser
"Our mass spectrometry and ensuing immunoprecipitation analysis indicated that UCHL3 could interact with DNA-PKcs, possibly suggesting context specific functions of UCHL3 for maintaining genome integrity."
30559450
UCHL3 binds PRKDC and Ku. 1 / 1
| 1
sparser
"These data thus suggested that UCHL3 interacts with Ku and/or DNA-PKcs in cells and could have a role at sites of DSBs in a catalytic-activity-dependent manner."
30559450
NFE2L2 affects UCHL3
| 1 1
NFE2L2 binds UCHL3.
| 1
UCHL3 binds NFE2L2. 1 / 1
| 1
sparser
"Mechanistically, co-immunoprecipitation assay showed that UCHL3 directly interacted with NRF2."
36527747
NFE2L2 activates UCHL3.
| 1
NFE2L2 activates UCHL3. 1 / 1
| 1
reach
"Moreover, we revealed that overexpression of NRF2 could antagonize the function of UCHL3 knockdown in cervical cancer cells."
36527747
LMO7 affects UCHL3
| 1 1
UCHL3 binds LMO7. 1 / 1
| 1
reach
"We suggest that the increase in severity may reflect an interaction between Uchl3 and Lmo7 in the ubiquitin mediated protein degradation pathway."
12761045
Ubiquitin binds UCHL3 and LMO7. 1 / 1
| 1
sparser
"We suggest that the increase in severity may reflect an interaction between Uchl3 and Lmo7 in the ubiquitin-mediated protein degradation pathway."
12761045
Ku affects UCHL3
| 2
UCHL3 binds PRKDC and Ku. 1 / 1
| 1
sparser
"These data thus suggested that UCHL3 interacts with Ku and/or DNA-PKcs in cells and could have a role at sites of DSBs in a catalytic-activity-dependent manner."
30559450
UCHL3 binds Ku. 1 / 1
| 1
sparser
"The immunoprecipitation of endogenous UCHL3 using an anti-UCHL3 antibody followed by immunoblotting analysis detected the interaction with endogenous Ku80, providing further support that UCHL3 interacts with Ku (Fig.  xref )."
30559450
CDH1 affects UCHL3
1 | 1
CDH1 inhibits UCHL3.
| 1
CDH1 inhibits UCHL3. 1 / 1
| 1
reach
"As shown in Fig. 4 A, E-cadherin increased but vimentin decreased in UCH-L3 overexpressing PC3 cells compared to mock or vector control cells."
25194810
CDH1 binds UCHL3.
1 |
UCHL3 binds CDH1. 1 / 1
1 |
biogrid
No evidence text available
24798214
BAP1 affects UCHL3
| 2
UCHL3 binds BAP1 and FlaG. 1 / 1
| 1
sparser
"We observed that FLAG-BAP1 ( xref B, upper panel), but not FLAG-UCHL3 ( xref B, lower panel), directly binds to HA-KEAP1."
35052618
UCHL3 binds KEAP1 and BAP1. 1 / 1
| 1
sparser
"To examine whether BAP1 and UCHL3 directly interacts with KEAP1, we cotransfected pcDNA3-FLAG-BAP1 or pcDNA3-FLAG-UCHL3 with pcDNA3-HA-KEAP1 in 293T cells and immunoprecipitated cell lysates with FLAG-agarose beads followed by Western blot analysis with HA-HRP antibody."
35052618
ATXN7L3 affects UCHL3
1 1 |
UCHL3 binds ATXN7L3. 2 / 2
1 1 |
biogrid
No evidence text available
16713569
hprd
No evidence text available
16713569
Δ affects UCHL3
| 1
Δ inhibits UCHL3. 1 / 1
| 1
reach
"Treatment with Δ12-PGJ2 in cells also inhibited UCH-L1 and UCH-L3 without alteration of proteasomal activity, indicating that prostaglandins can be suitable for neural disorder therapy [95]."
| PMC
Undecane affects UCHL3
1 |
Undecane decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
17337753
Ubiquitin substrate ubiquitin-AMC affects UCHL3
| 1
Ubiquitin substrate ubiquitin-AMC inhibits UCHL3. 1 / 1
| 1
eidos
"As an additional control experiment , we measured a Ki of 0.27 muM for the inhibition of UCH-L3 by ubiquitin with the substrate ubiquitin-AMC ."
16905103
Trimellitic anhydride increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
19042947
Trifluoperazine affects UCHL3
| 1
Trifluoperazine inhibits UCHL3. 1 / 1
| 1
reach
"Pimozide, flupenthixol and trifluoperazine were poor inhibitors of UCH-L1 and UCH-L3 (IC 50> 500 muM)."
22118673
Trichloroethene affects UCHL3
1 |
Trichloroethene methylates UCHL3. 1 / 1
1 |
ctd
No evidence text available
27618143
Titanium dioxide affects UCHL3
1 |
Titanium dioxide increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
30910687
Sodium arsenite affects UCHL3
1 |
Sodium arsenite increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
29361514
Silicon dioxide affects UCHL3
1 |
Silicon dioxide decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
19073995
Senecionine affects UCHL3
1 |
Senecionine increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
26100227
Sarin affects UCHL3
1 |
Sarin decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
19522546
Reported Akt affects UCHL3
| 1
Reported Akt activates UCHL3. 1 / 1
| 1
eidos
"Here we report that perifosine , a previously reported Akt inhibitor , can inhibit UCHL3 in vitro and in vivo ."
31113933
Quercetin affects UCHL3
1 |
Quercetin decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
21632981
Prostaglandin J2 affects UCHL3
| 1
reach
"Neither PGJ2 nor PGA1, D2, and E2 were found to inhibit UCH-L1 and UCH-L3."
15194490
Probe-UCHL3 complexes affects UCHL3
| 1
Probe-UCHL3 complexes inhibits UCHL3. 1 / 1
| 1
reach
"As shown in Figures XREF_FIG a, b and S2, the probe-UCHL3 complexes are completely reduced, yielding unlabeled UCHL3 and reduced probe (Ub SH)."
28841265
Polyubiquitin affects UCHL3
| 1
UCHL3 binds polyubiquitin. 1 / 1
| 1
sparser
"UCH-L3 binds to K48-linked polyubiquitin to protect against degradation and inhibit hydrolytic enzyme activity [ xref ]."
32486158
Pimozide affects UCHL3
| 1
Pimozide inhibits UCHL3. 1 / 1
| 1
reach
"Pimozide, flupenthixol and trifluoperazine were poor inhibitors of UCH-L1 and UCH-L3 (IC 50> 500 muM)."
22118673
Phosphorylation affects UCHL3
| 1
Phosphorylation inhibits UCHL3. 1 / 1
| 1
eidos
"Furthermore , this phosphorylation destabilizes UCHL3 , despite having no effect on its catalytic activity ."
30559450
Phorbol 13-acetate 12-myristate increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
25613284
Phlorizin affects UCHL3
1 |
Phlorizin decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
22538082
Perifosine affects UCHL3
| 1
Perifosine inhibits UCHL3. 1 / 1
| 1
eidos
"Here we report that perifosine , a previously reported Akt inhibitor , can inhibit UCHL3 in vitro and in vivo ."
31113933
Paracetamol affects UCHL3
1 |
Paracetamol increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
22230336
Microcystin affects UCHL3
1 |
Microcystin decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
22430071
Methylmercury chloride decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
23458150
Methapyrilene affects UCHL3
1 |
Methapyrilene increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
30467583
Jinfukang affects UCHL3
1 |
Jinfukang decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
27392435
Ionomycin affects UCHL3
1 |
Ionomycin increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
25613284
Hsa-miR-8082 affects UCHL3
1 |
Hsa-miR-8082 decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
19536157
Hsa-miR-7156-5p affects UCHL3
1 |
Hsa-miR-7156-5p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
22012620
Hsa-miR-6781-3p affects UCHL3
1 |
Hsa-miR-6781-3p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
19536157
Hsa-miR-6507-3p affects UCHL3
1 |
Hsa-miR-6507-3p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
22012620
Hsa-miR-616-5p affects UCHL3
1 |
Hsa-miR-616-5p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
22012620
Hsa-miR-606 affects UCHL3
1 |
Hsa-miR-606 decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
19536157
Hsa-miR-548p affects UCHL3
1 |
Hsa-miR-548p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
22012620
Hsa-miR-532-5p affects UCHL3
1 |
Hsa-miR-532-5p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
19536157
Hsa-miR-514b-5p affects UCHL3
1 |
Hsa-miR-514b-5p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
22012620
Hsa-miR-513c-5p affects UCHL3
1 |
Hsa-miR-513c-5p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
22012620
Hsa-miR-5093 affects UCHL3
1 |
Hsa-miR-5093 decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
19536157
Hsa-miR-4633-5p affects UCHL3
1 |
Hsa-miR-4633-5p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
19536157
Hsa-miR-4534 affects UCHL3
1 |
Hsa-miR-4534 decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
19536157
Hsa-miR-4273 affects UCHL3
1 |
Hsa-miR-4273 decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
22012620
Hsa-miR-375 affects UCHL3
1 |
Hsa-miR-375 decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20215506
Hsa-miR-373-5p affects UCHL3
1 |
Hsa-miR-373-5p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
22012620
Hsa-miR-372-5p affects UCHL3
1 |
Hsa-miR-372-5p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
22012620
Hsa-miR-371b-5p affects UCHL3
1 |
Hsa-miR-371b-5p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
22012620
Hsa-miR-371a-5p affects UCHL3
1 |
Hsa-miR-371a-5p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
22012620
Hsa-miR-3688-3p affects UCHL3
1 |
Hsa-miR-3688-3p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
19536157
Hsa-miR-3134 affects UCHL3
1 |
Hsa-miR-3134 decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
19536157
Hsa-miR-3124-3p affects UCHL3
1 |
Hsa-miR-3124-3p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
19536157
Hsa-miR-2682-3p affects UCHL3
1 |
Hsa-miR-2682-3p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
19536157
Hsa-miR-2117 affects UCHL3
1 |
Hsa-miR-2117 decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
22012620
Hsa-miR-183-5p affects UCHL3
1 |
Hsa-miR-183-5p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23622248
Hsa-miR-130b-5p affects UCHL3
1 |
Hsa-miR-130b-5p decreases the amount of UCHL3. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
19536157
Hexabromocyclododecane decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
29177809
Gentamycin affects UCHL3
1 |
Gentamycin increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
22061828
Farrerol affects UCHL3
| 1
Farrerol activates UCHL3. 1 / 1
| 1
reach
"Farrerol directly activates the deubiqutinase UCHL3 to promote DNA repair and reprogramming when mediated by somatic cell nuclear transfer."
37012254
Endosulfan affects UCHL3
1 |
Endosulfan increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
29391264
Electrophilic reagent inhibits UCHL3. 1 / 1
| 1
sparser
"These endogenous electrophiles (1) inhibit ubiquitin isopeptidase activity [ xref ] as well as ubiquitin hydrolases UCH-L1 and UCH-L3 [ xref ], (2) induce the formation of cysteine-targeted thyolation of UCH-L1 [ xref ] and of PGJ2/proteasome conjugates [ xref ], (3) trigger the oxidation of the S6 ATPase subunit of the 26S proteasome [ xref ], and disrupt 26S proteasome assembly [ xref ]."
19630993
Dorsomorphin affects UCHL3
1 |
Dorsomorphin increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
27188386
Diuron affects UCHL3
1 |
Diuron increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
21551480
Dicrotophos affects UCHL3
1 |
Dicrotophos decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
28302478
Dibutyl phthalate affects UCHL3
1 |
Dibutyl phthalate decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
17361019
Dibenzothiophene affects UCHL3
1 |
Dibenzothiophene decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
23656968
Diarsenic trioxide increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
25419056
Copper(II) sulfate increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
19549813
Chlorpyrifos affects UCHL3
1 |
Chlorpyrifos decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
18668222
Chalcone affects UCHL3
| 1
Chalcone inhibits UCHL3. 1 / 1
| 1
reach
"RA-9, a chalcone derivative with a structure similar to b-AP15, was reported to inhibit proteasomal DUBs [XREF_BIBR] as well as UCHL1, UCHL3, USP2, USP5, and USP8 [XREF_BIBR]."
29039082
Bisphenol A affects UCHL3
1 |
Bisphenol A increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
25181051
Bis(2-ethylhexyl) phthalate increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
19850644
Binding catalytic cysteine affects UCHL3
| 1
Binding catalytic cysteine inhibits UCHL3. 1 / 1
| 1
eidos
"Using our Ub tools to profile UCHL3 's activity , we generate a quantitative kinetic model of the inhibitory mechanism and we find that K27 Ub 2 can inhibit UCHL3 covalently , by binding to its catalytic cysteine , and allosterically , by locking its catalytic loop tightly in place ."
33238157
Apo affects UCHL3
| 1
UCHL3 binds apo. 1 / 1
| 1
sparser
"In the apo form of UCHL3, the closest homolog of UCH37, the loop is disordered but becomes ordered when ubiquitin is bound( xref , xref )."
23617878
Aldehyde affects UCHL3
| 1
Aldehyde inhibits UCHL3. 1 / 1
| 1
sparser
"We found complex viscosity dependencies and interpreted these in the context of a model in which association and acylation are viscosity-dependent but deacylation is viscosity-independent. (2) The kinetics of inhibition of UCH-L3 by ubiquitin C-terminal aldehyde (Ub-H) were determined and reveal a Ki that is less than 10(-14) M. Several mechanisms are considered to account for the extreme inhibition. (3) The IPaseT-catalyzed hydrolysis of Ub-AMC is modulated by Ub with activation at low [Ub] and inhibition at high [Ub]. (4) Finally, we compare kc/Km values for deubiquitinating enzyme-catalyzed hydrolysis of Ub-AMC and Z-Leu-Arg-Gly-Gly-AMC."
9485312
Aflatoxin B1 affects UCHL3
1 |
Aflatoxin B1 increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
27153756
ZYX affects UCHL3
1 |
UCHL3 binds ZYX. 1 / 1
1 |
biogrid
No evidence text available
22939629
ZRANB2 affects UCHL3
1 |
UCHL3 binds ZRANB2. 1 / 1
1 |
biogrid
No evidence text available
22939629
ZNRD2 affects UCHL3
1 |
ZNRD2 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
ZNF593 affects UCHL3
1 |
UCHL3 binds ZNF593. 1 / 1
1 |
biogrid
No evidence text available
22939629
ZIC1 affects UCHL3
1 |
ZIC1 decreases the amount of UCHL3. 1 / 1
1 |
biopax:msigdb
No evidence text available
ZFYVE19 affects UCHL3
1 |
UCHL3 binds ZFYVE19. 1 / 1
1 |
biogrid
No evidence text available
22939629
YAP1 affects UCHL3
1 |
UCHL3 binds YAP1. 1 / 1
1 |
biogrid
No evidence text available
22939629
XIAP affects UCHL3
1 |
UCHL3 binds XIAP. 1 / 1
1 |
biogrid
No evidence text available
22939629
Vehicle Emissions affects UCHL3
1 |
Vehicle Emissions demethylates UCHL3. 1 / 1
1 |
ctd
No evidence text available
25560391
VPS33B affects UCHL3
1 |
UCHL3 binds VPS33B. 1 / 1
1 |
biogrid
No evidence text available
22939629
VIM affects UCHL3
| 1
UCHL3 binds VIM. 1 / 1
| 1
reach
"co-immunoprecipitation, Immunofluorescence, and IHC were used to detect the interaction and expression association between UCHL3 and Vimentin in the cells."
36969045
VEA-260 affects UCHL3
| 1
VEA-260 inhibits UCHL3. 1 / 1
| 1
reach
"In contrast, hSENP6 (a homolog of PfSENP2) and UCH-L3 (a DUB) were not inhibited by VEA-260."
21700207
Ubiquitin affects NEDD8
| 1
Ubiquitin binds UCHL3 and NEDD8. 1 / 1
| 1
sparser
"In vitro GST pull-down assay demonstrated that UCH-L3 bound to both NEDD8 and ubiquitin."
9790970
Ubiquitin affects LMO7
| 1
Ubiquitin binds UCHL3 and LMO7. 1 / 1
| 1
sparser
"We suggest that the increase in severity may reflect an interaction between Uchl3 and Lmo7 in the ubiquitin-mediated protein degradation pathway."
12761045
UbVME affects UCHL3
| 1
UCHL3 binds UbVME. 1 / 1
| 1
reach
"In contrast, when UbVME binds to UCH-L3, the S shaped loop (153-167) is reordered and helix-6 ' (142-152) forms and covers the active site bound to the C-terminus of ubiquitin [13]."
19836345
UbV affects UCHL3
| 1
UCHL3 binds UbV. 1 / 1
| 1
sparser
"The WT-Ub-PRG labels several DUBs across a broad range of molecular weights, while the HA-UbV Q40V/T66K/V70F -PRG labels apparently a single band that corresponds to the molecular weight of the UCHL3-UbV adduct in the normal exposure blot ( xref A, top)."
35053210
USP9X affects UCHL3
1 |
UCHL3 binds USP9X. 1 / 1
1 |
biogrid
No evidence text available
22939629
USP7 affects USP2
| 1
UCHL3 binds USP14, USP2, and USP7. 1 / 1
| 1
sparser
"Binding of the C-terminal Ub segment in an extended conformation in the active-site cleft is a common feature of DUBs, as observed in the crystal structures of Ub derivatives in complex with UCH-L3, HAUSP, USP2, and USP14 (Hu et al., 2002, Hu et al., 2005, Misaghi et al., 2004, Renatus et al., 2006)."
17349955
USP48 affects UCHL3
1 |
UCHL3 binds USP48. 1 / 1
1 |
biogrid
No evidence text available
22939629
USP4 affects UCHL3
| 1
UCHL3 binds USP4 and NEDD8. 1 / 1
| 1
sparser
"NEDD8, UCHL3 and USP4 are highly associated with ubiquitination."
24093944
USP34 affects UCHL3
1 |
UCHL3 binds USP34. 1 / 1
1 |
biogrid
No evidence text available
22939629
USP28 affects UCHL3
1 |
UCHL3 binds USP28. 1 / 1
1 |
biogrid
No evidence text available
22626734
USP21 affects UCHL3
1 |
UCHL3 binds USP21. 1 / 1
1 |
biogrid
No evidence text available
22626734
USP20 affects UCHL3
1 |
UCHL3 binds USP20. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP2 affects USP7
| 1
UCHL3 binds USP14, USP2, and USP7. 1 / 1
| 1
sparser
"Binding of the C-terminal Ub segment in an extended conformation in the active-site cleft is a common feature of DUBs, as observed in the crystal structures of Ub derivatives in complex with UCH-L3, HAUSP, USP2, and USP14 (Hu et al., 2002, Hu et al., 2005, Misaghi et al., 2004, Renatus et al., 2006)."
17349955
USP15 affects UCHL3
1 |
UCHL3 binds USP15. 1 / 1
1 |
biogrid
No evidence text available
22626734
USP14-3 affects UCHL3
| 1
USP14-3 activates UCHL3. 1 / 1
| 1
reach
"Inhibition of deubiquitinating activity by USP14 aptamers was specific to USP14, with the USP14-3 aptamer failing to inhibit the DUB activity of UCHL3, USP47, USP5, and UCHL5 and UCH37 (XREF_FIG)."
26041011
USP14 affects USP7
| 1
UCHL3 binds USP14, USP2, and USP7. 1 / 1
| 1
sparser
"Binding of the C-terminal Ub segment in an extended conformation in the active-site cleft is a common feature of DUBs, as observed in the crystal structures of Ub derivatives in complex with UCH-L3, HAUSP, USP2, and USP14 (Hu et al., 2002, Hu et al., 2005, Misaghi et al., 2004, Renatus et al., 2006)."
17349955
UNK affects UCHL3
1 |
UCHL3 binds UNK. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3
1 |
UCHL3 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
15531586
UCHL3 affects ubiquitination TDP1 leading TDP1
| 1
UCHL3 inhibits ubiquitination TDP1 leading TDP1. 1 / 1
| 1
eidos
"UCHL3 decreases the ubiquitination level of TDP1 leading to the inhibition of TDP1 degradation ."
31967940
UCHL3 inhibits ubiquitin carboxyl-terminal hydrolase. 1 / 1
| 1
reach
"The researchers were surprised to discover that their C-terminally propargylated Ub (Ub-Prg), originally synthesized for site specific ubiquitination of peptides, inhibited the human DUB ubiquitin carboxyl-terminal hydrolase isoenzyme L3 (UCHL3)."
26933738
UCHL3 affects subsequent removal DNA
| 1
UCHL3 activates subsequent removal DNA. 1 / 1
| 1
eidos
"Lastly , we provide evidence showing that DNA damage-induced UCHL3 phosphorylation ( which is dependent on UCHL3 catalytic activity , ATM kinase and downstream NHEJ factors ) promotes destabilization of UCHL3 , suggesting that UCHL3 may dissociate from Ku80 to allow Ku ubiquitylation and subsequent removal from DNA ."
30559450
UCHL3 affects stemness ATP-binding cassette subfamily member
| 1
UCHL3 activates stemness ATP-binding cassette subfamily member. 1 / 1
| 1
eidos
"UCHL3 increased AhR stability and the binding of AhR to the promoter regions of the `` stemness '' genes ATP-binding cassette subfamily G member 2 ( ABCG2 ) , KLF4 , and c-Myc ."
32546741
UCHL3 affects stemness ABCG2
| 1
UCHL3 activates stemness ABCG2. 1 / 1
| 1
eidos
"Depletion of UCHL3 markedly downregulated the `` stemness '' genes ABCG2 , KLF4 , and c-Myc , leading to the loss of self-renewal and tumorigenesis in NSCLCs ."
32546741
UCHL3 affects stem-like characteristics potent tumorigenic capacity NSCLC cells
| 1
UCHL3 activates stem-like characteristics potent tumorigenic capacity NSCLC cells. 1 / 1
| 1
eidos
"Moreover , we showed that UCHL3 promotes the stem-like characteristics and potent tumorigenic capacity of NSCLC cells ."
32546741
UCHL3 affects sodium atom
| 1
UCHL3 activates sodium atom. 1 / 1
| 1
reach
"Whereas Usp10 has been implicated in removal of ubiquitin from CFTR during recycling [57], Usp33 and Usp20 have been proposed to remove ubiquitin from the recycling beta-adrenergic receptor [58] while[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22800866
UCHL3 affects repair
| 1
UCHL3 inhibits repair. 1 / 1
| 1
eidos
"Taken together , these data suggested that the AKT inhibitor perifosine has an off-target effect at low dose by targeting UCHL3 , which in turn impairs RAD51 foci formation and HR repair ."
31113933
UCHL3 affects regulation faithful genome segregation maintenance euploidy human cells
| 1
UCHL3 activates regulation faithful genome segregation maintenance euploidy human cells. 1 / 1
| 1
eidos
"Thus , UCHL3 contributes to the regulation of faithful genome segregation and maintenance of euploidy in human cells ."
32738097
UCHL3 affects radiosensitivity NSCLC cells
| 1
UCHL3 inhibits radiosensitivity NSCLC cells. 1 / 1
| 1
eidos
"UCHL3 reduced the radiosensitivity of NSCLC cells by stabilizing AhR protein ."
35918714
UCHL3 affects proteolysis
| 1
UCHL3 activates proteolysis. 1 / 1
| 1
reach
"UCHL3 was responsible for the disassembly of the ubiquitin protein complex or polyubiquitin chains during the substrate priming for proteasomal proteolysis to prevent inappropriate protein degradation [XREF_BIBR]."
27780264
reach
"Generally, these findings indicated that as a DUB of AhR, UCHL3 increases AhR protein stabilization in a DUB activity-dependent manner."
32546741
UCHL3 affects properties
| 1
UCHL3 activates properties. 1 / 1
| 1
eidos
"Moreover , the modulation of CSC-like properties by UCH-L1 and UCH-L3 was through the PI3 K / Akt signaling pathway ."
34745437
UCHL3 affects polyubiquitin
| 1
UCHL3 binds polyubiquitin. 1 / 1
| 1
sparser
"UCH-L3 binds to K48-linked polyubiquitin to protect against degradation and inhibit hydrolytic enzyme activity [ xref ]."
32486158
UCHL3 affects polyubiquitin chain
| 1
UCHL3 inhibits polyubiquitin chain. 1 / 1
| 1
reach
"The polyubiquitin chain is degraded by UCHL3 and further processed by de-ubiquitinating enzymes (DUBs) into monomeric ubiquitin."
26388726
UCHL3 affects pY701-STAT1
| 1
UCHL3 increases the amount of pY701-STAT1. 1 / 1
| 1
reach
"The result showed that UCHL3 overexpression robustly increased the level of pY701-STAT1 (p-STAT1) induced by IFNalpha in a time dependent manner."
28583475
UCHL3 affects ossification
| 1
UCHL3 activates ossification. 1 / 1
| 1
reach
"This study suggests that mechanical force-induced macrophage-derived exosomal UCHL3 promotes BMSCs osteogenesis by targeting SMAD1, thereby promoting alveolar bone formation during OTM."
36915132
UCHL3 affects neddylation
| 1
UCHL3 inhibits neddylation. 1 / 1
| 1
sparser
"We reveal that UCHL3 can inhibit Cullin1 neddylation in cells dependently on COPS5."
28583475
UCHL3 affects monoubiquitin
| 1
UCHL3 activates monoubiquitin. 1 / 1
| 1
reach
"Recent evidence suggests that monoubiquitin release from its precursors is mediated predominantly by five main DUBs : ubiquitin thioesterase otulin, UCHL3, USP5, USP7 and USP9X [XREF_BIBR], although each has multiple substrates that can be targeted for deubiquitination to maintain the free ubiquitin pool."
29543760
UCHL3 affects mono-Ub
| 1
UCHL3 activates mono-Ub. 1 / 1
| 1
reach
"After incubation for 24 h, UCH-L3 did not produce mono-Ub from these three forms of di-Ub (data not shown), which is consistent with previous reports (Larsen et al., 1998)."
19154770
UCHL3 affects migration
| 1
UCHL3 activates migration. 1 / 1
| 1
eidos
"In summary , this study reveals that knockdown of UCHL3 inhibits ESCC growth and migration by reducing CRY2 methylation through downregulation of FOXM1 expression ."
35088238
UCHL3 affects metastasis gastric cancer
| 1
UCHL3 activates metastasis gastric cancer. 1 / 1
| 1
eidos
"UCHL3 stimulates metastasis of gastric cancer through upregulating IGF2 ."
34278751
UCHL3 affects localization
| 1
UCHL3 activates localization. 1 / 1
| 1
reach
"Instead, UCHL3 promotes localization of Aurora B to kinetochores, suggesting its role in the error correction mechanism monitoring bi-orientation of chromosomes during metaphase."
32738097
UCHL3 affects localization Aurora B kinetochores
| 1
UCHL3 activates localization Aurora B kinetochores. 1 / 1
| 1
eidos
"Instead , UCHL3 promotes localization of Aurora B to kinetochores , suggesting its role in the error correction mechanism monitoring bi-orientation of chromosomes during metaphase ."
32738097
UCHL3 affects independent repair pathways
| 1
UCHL3 activates independent repair pathways. 1 / 1
| 1
eidos
"Although it will be intriguing to reveal how UCHL3 may contribute to independent DSB repair pathways , our findings in this study highlight the importance of UCHL3 for maintaining genome integrity by regulating the major DSB repair pathway , c-NHEJ ."
30559450
UCHL3 activates glycolytic process. 1 / 1
| 1
reach
"UCHL3 promotes aerobic glycolysis of pancreatic cancer through upregulating LDHA expression."
33616859
UCHL3 affects farrerol
| 1
UCHL3 inhibits farrerol. 1 / 1
| 1
reach
"Ablating UCHL3 significantly attenuates farrerol-mediated stimulation in HR and SCNT embryo development."
37012254
UCHL3 affects expansion cumulus cells
| 1
UCHL3 activates expansion cumulus cells. 1 / 1
| 1
eidos
"In vitro , inhibition of UCHL3 reduces the expansion of cumulus cells ( Mtango et al ., 2014 ) ."
33842483
UCHL3 inhibits embryo development. 1 / 1
| 1
reach
"Ablating UCHL3 significantly attenuates farrerol-mediated stimulation in HR and SCNT embryo development."
37012254
reach
"Additionally, depleting UCHL3 resulted in reduced Ku80 foci formation, Ku80 binding to chromatin after DSB induction, moderately sensitized cells to ionizing radiation and decreased NHEJ efficiencies."
30559450
UCHL3 affects differentiation osteoblast C2C12 cells
| 1
UCHL3 activates differentiation osteoblast C2C12 cells. 1 / 1
| 1
eidos
"UCH-L3 promotes the differentiation of osteoblast from C2C12 cells , while knockdown of Uch-l3 delays osteoblast differentiation [ 73 ] ."
30123285
UCHL3 affects cryptorchid-induced germ cell apoptosis mice
| 1
UCHL3 activates cryptorchid-induced germ cell apoptosis mice. 1 / 1
| 1
eidos
"Next , UCHL3 has also been demonstrated to alleviate cryptorchid-induced germ cell apoptosis in gad mice ."
33585209
UCHL3 affects colorectal cancer cells
| 1
UCHL3 activates colorectal cancer cells. 1 / 1
| 1
eidos
"UCHL3 promotes proliferation of colorectal cancer cells by regulating SOX12 via AKT / mTOR signaling pathway ."
33194042
UCHL3 affects cleave
| 1
UCHL3 activates cleave. 1 / 1
| 1
eidos
"It was reported that UCHL3 enables to cleave the C-terminus of NEDD8 , which is a ubiquitin-like protein that exerts the function of Ub to be conjugated to a lysine residue of the substrate ( 115 ) ."
33585209
UCHL3 affects chromosome alignment defects
| 1
UCHL3 inhibits chromosome alignment defects. 1 / 1
| 1
eidos
"Downregulation or inhibition of UCHL3 leads to chromosome alignment defects during metaphase ."
32738097
UCHL3 affects chromatin retention
| 1
UCHL3 activates chromatin retention. 1 / 1
| 1
eidos
"Taken together , these data suggest that UCHL3 could enhance Ku chromatin retention upon DSB induction by deubiquitylating Ku80 ."
30559450
UCHL3 affects cellular survival
| 1
UCHL3 activates cellular survival. 1 / 1
| 1
eidos
"UCHL3 promotes cellular survival after IR and DSB repair ."
30559450
UCHL3 affects binding AhR stemness
| 1
UCHL3 activates binding AhR stemness. 1 / 1
| 1
eidos
"UCHL3 increased AhR stability and the binding of AhR to the promoter regions of the `` stemness '' genes ATP-binding cassette subfamily G member 2 ( ABCG2 ) , KLF4 , and c-Myc ."
32546741
UCHL3 affects autophagy
| 1
UCHL3 activates autophagy. 1 / 1
| 1
reach
"Taken together, the results of the present study suggest that UCHL3 knockdown decreases melanoma cell proliferation by increasing cell autophagy through regulating NEDD8 expression and autophagosome numbers."
34539860
UCHL3 affects apoptotic process
| 1
reach
"Hence, loss of UCH-L3 leads to mitochondrial oxidative stress related photoreceptor cell apoptosis in a caspase independent manner."
16816367
UCHL3 affects apo
| 1
UCHL3 binds apo. 1 / 1
| 1
sparser
"In the apo form of UCHL3, the closest homolog of UCH37, the loop is disordered but becomes ordered when ubiquitin is bound( xref , xref )."
23617878
UCHL3 affects aerobic glycolysis pancreatic cancer
| 1
UCHL3 activates aerobic glycolysis pancreatic cancer. 1 / 1
| 1
eidos
"UCHL3 promotes aerobic glycolysis of pancreatic cancer through upregulating LDHA expression ."
33616859
UCHL3 affects activation
| 1
UCHL3 inhibits activation. 1 / 1
| 1
sparser
"Our data showed that knockdown of endogenous UCHL3 inhibited IFN-I induced transcription activation of all three ISGs ( Fig. 7 B, C and 7D), strongly suggesting that UCHL3 positively regulates IFN-I s[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28583475
UCHL3 affects acetaldehyde
| 1
Acetaldehyde binds UCHL3. 1 / 1
| 1
reach
"Binding of ubiquitin leads to apparent stabilization of the crossover loop, as it was seen straddling the active site in co-crystal structures of ubiquitin aldehyde bound to UCHL3 (XREF_FIG and XREF_FIG) and the yeast UCH Yuh1 XREF_BIBR, XREF_BIBR."
27012468
UCHL3 affects ZYX
1 |
UCHL3 binds ZYX. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects ZRANB2
1 |
UCHL3 binds ZRANB2. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects ZRANB1
| 1
UCHL3 activates ZRANB1. 1 / 1
| 1
reach
"Structure of a USP20 inhibitor from GSK.Figure 29 Structure of a USP30 inhibitor.Structures of UCHL1 inhibitors.VAE(OMe)-FMK Structure of a weak tripeptide FMK UCHL1 inhibitor.Figure 33 Structures of UCHL3 inhibitors.Structures of TRABID and RPN11 inhibitors."
26852935
UCHL3 affects ZNRD2
1 |
ZNRD2 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects ZNF593
1 |
UCHL3 binds ZNF593. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects ZFYVE19
1 |
UCHL3 binds ZFYVE19. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects XIAP
1 |
UCHL3 binds XIAP. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects VSV-G
| 1
Modified UCHL3 decreases the amount of VSV-G. 1 / 1
| 1
reach
"The result showed that UCHL3 overexpression decreased the levels of VSV-G to some extent, suggesting that UCHL3 promotes cellular antiviral ability under a basal IFN secreting environment."
28583475
UCHL3 affects VPS33B
1 |
UCHL3 binds VPS33B. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects UbVME
| 1
UCHL3 binds UbVME. 1 / 1
| 1
reach
"In contrast, when UbVME binds to UCH-L3, the S shaped loop (153-167) is reordered and helix-6 ' (142-152) forms and covers the active site bound to the C-terminus of ubiquitin [13]."
19836345
UCHL3 affects UbV
| 1
UCHL3 binds UbV. 1 / 1
| 1
sparser
"The WT-Ub-PRG labels several DUBs across a broad range of molecular weights, while the HA-UbV Q40V/T66K/V70F -PRG labels apparently a single band that corresponds to the molecular weight of the UCHL3-UbV adduct in the normal exposure blot ( xref A, top)."
35053210
UCHL3 affects Ub dimers
| 1
UCHL3 binds Ub dimers. 1 / 1
| 1
reach
"Here we show that Ub dimers interact non covalently with UCH-L3 in vitro and in cells."
19154770
UCHL3 affects USP9X
1 |
UCHL3 binds USP9X. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects USP7
| 1
UCHL3 binds USP14, USP2, and USP7. 1 / 1
| 1
sparser
"Binding of the C-terminal Ub segment in an extended conformation in the active-site cleft is a common feature of DUBs, as observed in the crystal structures of Ub derivatives in complex with UCH-L3, HAUSP, USP2, and USP14 (Hu et al., 2002, Hu et al., 2005, Misaghi et al., 2004, Renatus et al., 2006)."
17349955
UCHL3 affects USP48
1 |
UCHL3 binds USP48. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects USP4
| 1
UCHL3 binds USP4 and NEDD8. 1 / 1
| 1
sparser
"NEDD8, UCHL3 and USP4 are highly associated with ubiquitination."
24093944
UCHL3 affects USP34
1 |
UCHL3 binds USP34. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects USP28
1 |
UCHL3 binds USP28. 1 / 1
1 |
biogrid
No evidence text available
22626734
UCHL3 affects USP21
1 |
UCHL3 binds USP21. 1 / 1
1 |
biogrid
No evidence text available
22626734
UCHL3 affects USP20
1 |
UCHL3 binds USP20. 1 / 1
1 |
biogrid
No evidence text available
19615732
UCHL3 affects USP2
| 1
UCHL3 binds USP14, USP2, and USP7. 1 / 1
| 1
sparser
"Binding of the C-terminal Ub segment in an extended conformation in the active-site cleft is a common feature of DUBs, as observed in the crystal structures of Ub derivatives in complex with UCH-L3, HAUSP, USP2, and USP14 (Hu et al., 2002, Hu et al., 2005, Misaghi et al., 2004, Renatus et al., 2006)."
17349955
UCHL3 affects USP15
1 |
UCHL3 binds USP15. 1 / 1
1 |
biogrid
No evidence text available
22626734
UCHL3 affects USP14, USP2, and USP7
| 1
UCHL3 binds USP14, USP2, and USP7. 1 / 1
| 1
sparser
"Binding of the C-terminal Ub segment in an extended conformation in the active-site cleft is a common feature of DUBs, as observed in the crystal structures of Ub derivatives in complex with UCH-L3, HAUSP, USP2, and USP14 (Hu et al., 2002, Hu et al., 2005, Misaghi et al., 2004, Renatus et al., 2006)."
17349955
UCHL3 affects UNK
1 |
UCHL3 binds UNK. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects UCHL3 KO
| 1
UCHL3 activates UCHL3 KO. 1 / 1
| 1
reach
"These data were in line with observed c-NHEJ activities being reduced in UCHL3 KO#2 cells, which was largely rescued by exogenously expressed FLAG-UCHL3 (Fig. 3C)."
30559450
UCHL3 affects UCH
| 1
UCHL3 activates UCH. 1 / 1
| 1
reach
"This suggests that UCH functions associated with the egg cortex and oolemma may be at least partially restored by UCHL3 function, if opportunity for this is presented during oogenesis."
21678411
UCHL3 affects UCH Yuh1
| 1
UCHL3 binds UCH Yuh1. 1 / 1
| 1
reach
"Binding of ubiquitin leads to apparent stabilization of the crossover loop, as it was seen straddling the active site in co-crystal structures of ubiquitin aldehyde bound to UCHL3 (XREF_FIG and XREF_FIG) and the yeast UCH Yuh1 XREF_BIBR, XREF_BIBR."
27012468
UCHL3 affects UBXN7
1 |
UCHL3 binds UBXN7. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects UBR1
1 |
UCHL3 binds UBR1. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects UBQLN1
1 |
UBQLN1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects UBL7
1 |
UCHL3 binds UBL7. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects UBE4B
1 |
UBE4B binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects UBE2V1
1 |
UBE2V1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects UBE2S
1 |
UCHL3 binds UBE2S. 1 / 1
1 |
biogrid
No evidence text available
20622874
UCHL3 affects UBE2L3
1 |
UBE2L3 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects UBE2A
1 |
UBE2A binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects UBA1
1 |
UBA1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 activates Thyroid Carcinoma, Anaplastic. 1 / 1
| 1
reach
"The deubiquitinating enzyme UCHL3 promotes anaplastic thyroid cancer progression and metastasis through Hippo signaling pathway."
36813921
UCHL3 affects TYK2
| 1
UCHL3 increases the amount of TYK2. 1 / 1
| 1
reach
"Interestingly, we found that UCHL3 overexpression upregulated p-TYK2 levels induced by IFNalpha."
28583475
UCHL3 affects TWIST1
| 1
UCHL3 inhibits TWIST1. 1 / 1
| 1
reach
"We found that UCH-L3 knockdown in RWPE1 upregulates Snail, Slug, and Twist."
25194810
UCHL3 affects TTC9C
1 |
UCHL3 binds TTC9C. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects TTC1
1 |
TTC1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects TSN
1 |
TSN binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects TRM1
1 |
UCHL3 binds TRM1. 1 / 1
1 |
virhostnet
No evidence text available
31873071
UCHL3 affects TRIM63
1 |
UCHL3 binds TRIM63. 1 / 1
1 |
biogrid
No evidence text available
22626734
UCHL3 affects TRIM55
1 |
UCHL3 binds TRIM55. 1 / 1
1 |
biogrid
No evidence text available
22626734
UCHL3 affects TRIM54
1 |
UCHL3 binds TRIM54. 1 / 1
1 |
biogrid
No evidence text available
22626734
UCHL3 affects TRIM27
1 |
UCHL3 binds TRIM27. 1 / 1
1 |
biogrid
No evidence text available
23105109
UCHL3 affects TPD52L1
1 |
TPD52L1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects TPD52
1 |
TPD52 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects TEAD
| 1
UCHL3 increases the amount of TEAD. 1 / 1
| 1
reach
"Depletion of UCHL3 decreased the YAP protein level and the expression of YAP/TEAD target genes in ATC."
36813921
UCHL3 affects TAGLN2
1 |
TAGLN2 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects TAGLN
1 |
TAGLN binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
Mutated UCHL3 inhibits Side-Population Cells. 1 / 1
| 1
reach
"FACS analysis showed that the UCHL3 mutants reduced the number of SP cells among A549 cells (Fig. 6c)."
32546741
UCHL3 affects SUMO3
1 |
SUMO3 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects SUMO2
| 1
SUMO2 binds SUMO1 and UCHL3. 1 / 1
| 1
sparser
"In contrast, UCH-L3 did not bind to sentrin-1, sentrin-2, or sentrin-3."
9790970
UCHL3 affects SULT1A1
1 |
SULT1A1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects STUB1
| 1
UCHL3 deubiquitinates STUB1. 1 / 1
| 1
reach
"No other tested DUB behaved in a similar manner : USP5 deubiquitinated Ub-CHIP in an unregulated manner, while UCHL-1 and UCHL-3 did not deubiquitinate Ub-CHIP under any conditions (XREF_FIG)."
21855799
UCHL3 affects STIP1
1 |
STIP1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects SPOP
| 1
UCHL3 inhibits SPOP. 1 / 1
| 1
reach
"Interestingly, we found that the concomitant knockdown of SPOP with UCHL3 rescued the effect of UCHL3 knockdown on BRD4 protein (Fig. S4i), indicating that UCHL3 antagonizes SPOP to maintain BRD4 stability."
34290255
UCHL3 affects SOX12
| 1
UCHL3 activates SOX12. 1 / 1
| 1
reach
"UCHL3 promotes proliferation of colorectal cancer cells by regulating SOX12 via AKT and mTOR signaling pathway."
33194042
UCHL3 affects SOD2
1 |
SOD2 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects SNRPD1
1 |
SNRPD1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects SNAI2
| 1
UCHL3 inhibits SNAI2. 1 / 1
| 1
reach
"We found that UCH-L3 knockdown in RWPE1 upregulates Snail, Slug, and Twist."
25194810
UCHL3 affects SNAI1
| 1
UCHL3 inhibits SNAI1. 1 / 1
| 1
reach
"We found that UCH-L3 knockdown in RWPE1 upregulates Snail, Slug, and Twist."
25194810
UCHL3 affects SMS
1 |
SMS binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects SLC6A5
| 1
UCHL3 leads to the ubiquitination of SLC6A5. 1 / 1
| 1
reach
"Specific inhibition of UCHL1 with LDN-57444 and of UCHL3 with TCID diminished GlyT2 ubiquitination, which was more pronounced when UCHL1 was inhibited and when cells were exposed to these inhibitors for longer periods (XREF_FIG)."
23484054
UCHL3 affects SERPINB3
| 1
UCHL3 activates SERPINB3. 1 / 1
| 1
reach
"In summary, our experiments indicate that UCHL3 is a specific DUB for AhR and that UCHL3 elevates AhR protein stabilization through deubiquitination.We further detected mRNA levels in 35 paired ADC and SCC lung cancer and corresponding paracancerous normal tissues (Supplementary Fig. S4b, c) and noted no difference between histologic subtypes."
32546741
UCHL3 affects Rho-Ub-PA
| 1
UCHL3 binds Rho-Ub-PA. 1 / 1
| 1
reach
"The activity of UCHL3 was also monitored using the fluorescent Ub-propargylamide (PA) DUB ABP Rho-Ub-PA.3 d Fluorescent bands corresponding to UCHL3 bound to Rho-Ub-PA were observed for both unlabeled UCHL3 and DTT treated Ub SS -UCHL3 complexes, but not for untreated Ub SS -UCHL3 complexes."
28841265
UCHL3 affects Recurrence
| 1
UCHL3 inhibits Recurrence. 1 / 1
| 1
reach
"Overall, these results showed that UCH-L1 and UCH-L3 could be effective therapeutic targets to eliminate the recurrence caused by CSCs in prostate cancers."
34745437
UCHL3 affects RPN11 inhibitors
| 1
UCHL3 activates RPN11 inhibitors. 1 / 1
| 1
reach
"Structure of a USP20 inhibitor from GSK.Figure 29 Structure of a USP30 inhibitor.Structures of UCHL1 inhibitors.VAE(OMe)-FMK Structure of a weak tripeptide FMK UCHL1 inhibitor.Figure 33 Structures of UCHL3 inhibitors.Structures of TRABID and RPN11 inhibitors."
26852935
UCHL3 affects RDX
1 |
UCHL3 binds RDX. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects RAD51B
1 |
UCHL3 deubiquitinates RAD51B. 1 / 1
1 |
ubibrowser
"Mechanistically, in response to DNA damage, the deubiquitinase UCHL3 is phosphorylated and activated by ATM. UCHL3, in turn, deubiquitinates RAD51 and promotes the binding between RAD51 and BRCA2."
27941124
UCHL3 affects RAD51 foci
| 1
UCHL3 inhibits RAD51 foci. 1 / 1
| 1
eidos
"Taken together , these data suggested that the AKT inhibitor perifosine has an off-target effect at low dose by targeting UCHL3 , which in turn impairs RAD51 foci formation and HR repair ."
31113933
UCHL3 affects Proteasome
| 1
UCHL3 inhibits Proteasome. 1 / 1
| 1
reach
"Inhibiting the proteasome using MG132 led to marked enrichment of K48 ubiquitylated TDP1 in control cells but no impact in rhabdomyosarcoma cells, due to high UCHL3 levels (XREF_FIG C)."
29898404
UCHL3 affects Polyubiquitin
| 1
UCHL3 deubiquitinates Polyubiquitin 1 / 1
| 1
reach
"UCH-L3 physically interacts with Smad1 and stabilizes it by deubiquitylating its polyubiquitin."
30123285
UCHL3 affects PPIB
1 |
UCHL3 binds PPIB. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects POGK
1 |
UCHL3 binds POGK. 1 / 1
1 |
biogrid
No evidence text available
21900206
UCHL3 affects PLA2G2A
1 |
UCHL3 binds PLA2G2A. 1 / 1
1 |
biogrid
No evidence text available
22118674
UCHL3 affects PJA1
1 |
UCHL3 binds PJA1. 1 / 1
1 |
biogrid
No evidence text available
22626734
UCHL3 affects PI3
| 1
UCHL3 inhibits PI3. 1 / 1
| 1
reach
"The NVP-BEZ235 treatment inhibited the downstream effectors of the PI3 K/Akt pathway activated by UCH-L1 introduction and UCH-L3 depletion (Figure 3(B))."
34745437
UCHL3 affects PGD
1 |
UCHL3 binds PGD. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects PEBP1
1 |
UCHL3 binds PEBP1. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects PCMT1
1 |
UCHL3 binds PCMT1. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects PBX1
1 |
UCHL3 binds PBX1. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects P4HB
1 |
UCHL3 binds P4HB. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects OTULIN
| 1
UCHL3 binds OTULIN. 1 / 1
| 1
reach
"His PD showed that OTULIN ABPDeltaG76 still bound to USP5 and UCHL3 (XREF_FIG I), confirming our MS results that these DUBs are associating largely in a non covalent manner with the probes (compare XREF_FIG B and 6C)."
28919039
UCHL3 affects NUP93
1 |
UCHL3 binds NUP93. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects NT5DC1
1 |
UCHL3 binds NT5DC1. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects NSG2
1 |
UCHL3 binds NSG2. 1 / 1
1 |
biogrid
No evidence text available
21900206
UCHL3 affects NFkappaB
| 1
UCHL3 activates NFkappaB. 1 / 1
| 1
eidos
"Moreover , UCH-L3 was reported to activate NF-kappaB signaling by deubiquitinating and stabilizing the ubiquitin ligase TRAF2 ( Zhang et al ., 2020 ) ."
36340031
UCHL3 affects NFKBIA
| 1
UCHL3 increases the amount of NFKBIA. 1 / 1
| 1
reach
"The results showed that UCHL3 upregulated protein levels of IkappaBalpha."
28583475
UCHL3 affects Melanoma
| 1
UCHL3 activates Melanoma. 1 / 1
| 1
reach
"Taken together, the results of the present study suggest that UCHL3 knockdown decreases melanoma cell proliferation by increasing cell autophagy through regulating NEDD8 expression and autophagosome numbers."
34539860
UCHL3 affects MSN
1 |
UCHL3 binds MSN. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects MCL1
| 1
UCHL3 deubiquitinates MCL1. 1 / 1
| 1
reach
"Cdu1, but not the control enzyme UCH-L3 strongly deubiquitinated Mcl-1 (XREF_FIG)."
28347402
UCHL3 affects MAPRE1
1 |
UCHL3 binds MAPRE1. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects LONRF1
1 |
UCHL3 binds LONRF1. 1 / 1
1 |
biogrid
No evidence text available
23105109
UCHL3 affects LDHA
| 1
UCHL3 increases the amount of LDHA. 1 / 1
| 1
reach
"Further investigations revealed that LDHA expression was promoted by UCHL3 and could be reduced by shFOXM1, and that low expressed LDHA partly reversed the inhibition of aerobic glycolysis induced by overexpressed UCHL3."
33616859
UCHL3 affects Ku80 foci
| 1
UCHL3 activates Ku80 foci. 1 / 1
| 1
eidos
"Additionally , depleting UCHL3 resulted in reduced Ku80 foci formation , Ku80 binding to chromatin after DSB induction , moderately sensitized cells to ionizing radiation and decreased NHEJ efficiencies ."
30559450
UCHL3 affects Ku80 IRIF
| 1
UCHL3 inhibits Ku80 IRIF. 1 / 1
| 1
eidos
"Furthermore , depletion or knocking out of UCHL3 resulted in reduced Ku80 IRIF and caused moderately increased sensitivity to IR , which was not additive to the cellular sensitivity observed upon XRCC4 depletion ."
30559450
UCHL3 affects Ku ubiquitylation
| 1
UCHL3 activates Ku ubiquitylation. 1 / 1
| 1
eidos
"Lastly , we provide evidence showing that DNA damage-induced UCHL3 phosphorylation ( which is dependent on UCHL3 catalytic activity , ATM kinase and downstream NHEJ factors ) promotes destabilization of UCHL3 , suggesting that UCHL3 may dissociate from Ku80 to allow Ku ubiquitylation and subsequent removal from DNA ."
30559450
UCHL3 affects KLF4
| 1
UCHL3 increases the amount of KLF4. 1 / 1
| 1
reach
"As expected, overexpression of UCHL3 increased the expression of ABCG2, c-Myc and KLF4, genes associated with stemness, at the protein level in A549 and H1299 cells (Fig. 5b, c), while depletion of UCHL3 decreased their protein expression (Fig. 5d)."
32546741
UCHL3 affects KEAP1
| 1
UCHL3 binds KEAP1 and BAP1. 1 / 1
| 1
sparser
"To examine whether BAP1 and UCHL3 directly interacts with KEAP1, we cotransfected pcDNA3-FLAG-BAP1 or pcDNA3-FLAG-UCHL3 with pcDNA3-HA-KEAP1 in 293T cells and immunoprecipitated cell lysates with FLAG-agarose beads followed by Western blot analysis with HA-HRP antibody."
35052618
UCHL3 affects Interferon
| 1
UCHL3 activates Interferon. 1 / 1
| 1
reach
"Given that we demonstrated that UCHL3 promoted activation of IFN signaling, we observed whether IFN-I could affect the interaction between UCHL3 and COPS5."
28583475
UCHL3 affects ISYNA1
1 |
UCHL3 binds ISYNA1. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects ISRE Luciferase
| 1
UCHL3 activates ISRE Luciferase. 1 / 1
| 1
reach
"We found that knockdown of endogenous UCHL3 reduced the ISRE Luciferase activity in IFN signaling."
28583475
UCHL3 affects IR-induced DNA
| 1
UCHL3 activates IR-induced DNA. 1 / 1
| 1
eidos
"Furthermore , gammaH2AX foci staining and Western blot analysis showed that knockdown of UCHL3 increased IR-induced DNA damage ."
34016790
UCHL3 affects IMPDH2
1 |
UCHL3 binds IMPDH2. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects IGF1R
| 1
Modified UCHL3 leads to the phosphorylation of IGF1R. 1 / 1
| 1
reach
"Moreover, ectopic expression of wild-type UCH-L3 restored the phosphorylation of insulin and IGF-I receptor and adipocyte differentiation in Uchl3 (-/-) MEFs."
19837878
UCHL3 affects IFNAR2
| 1
UCHL3 activates IFNAR2. 1 / 1
| 1
reach
"This finding suggests that UCHL3 could target either TYK2 itself or IFN-I receptors (IFNAR1 and IFNAR2), which are the direct proteins upstream of TYK2 as well as the first signaling proteins in IFN-I[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28583475
UCHL3 affects IFN-I receptors
| 1
UCHL3 activates IFN-I receptors. 1 / 1
| 1
reach
"This finding suggests that UCHL3 could target either TYK2 itself or IFN-I receptors (IFNAR1 and IFNAR2), which are the direct proteins upstream of TYK2 as well as the first signaling proteins in IFN-I[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28583475
UCHL3 affects HSD17B10
1 |
UCHL3 binds HSD17B10. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects HINT1
1 |
UCHL3 binds HINT1. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects HA-Ub
| 1
UCHL3 binds HA-Ub. 1 / 1
| 1
reach
"The blot shows full formation of the HA-Ub and UCHL3 complex at all concentrations after preincubation with 1 indicating the molecule does not inhibit UCHL3 in cells (XREF_FIG B, second panel)."
33668938
UCHL3 affects GST
| 1
GST binds UCHL3. 1 / 1
| 1
sparser
"We observed a reduced ubiquitin signal with GST-UCHL3 but not with GST, suggesting that UCHL3 directly removed ubiquitin from Ku80."
30559450
UCHL3 affects GPX4
1 |
UCHL3 binds GPX4. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects GDI1
1 |
UCHL3 binds GDI1. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects GADL1
| 1
UCHL3 activates GADL1. 1 / 1
| 1
reach
"In summary, our experiments indicate that UCHL3 is a specific DUB for AhR and that UCHL3 elevates AhR protein stabilization through deubiquitination.We further detected mRNA levels in 35 paired ADC and SCC lung cancer and corresponding paracancerous normal tissues (Supplementary Fig. S4b, c) and noted no difference between histologic subtypes."
32546741
UCHL3 affects FH
| 1
UCHL3 binds FH. 1 / 1
| 1
sparser
"The data showed the interaction between FH-UCHL3 and endogenous COPS5 ( Fig. 4 A)."
28583475
eidos
"Knockdown of UCHL3 inhibits esophageal squamous cell carcinoma progression by reducing CRY2 methylation ."
35088238
UCHL3 affects EZR
1 |
UCHL3 binds EZR. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects ESCC growth
| 1
UCHL3 activates ESCC growth. 1 / 1
| 1
eidos
"In summary , this study reveals that knockdown of UCHL3 inhibits ESCC growth and migration by reducing CRY2 methylation through downregulation of FOXM1 expression ."
35088238
UCHL3 affects ENaC
| 1
UCHL3 activates ENaC. 1 / 1
| 1
reach
"Whereas Usp10 has been implicated in removal of ubiquitin from CFTR during recycling [57], Usp33 and Usp20 have been proposed to remove ubiquitin from the recycling beta-adrenergic receptor [58] while[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22800866
UCHL3 affects ENO2
1 |
UCHL3 binds ENO2. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects ENO1
1 |
UCHL3 binds ENO1. 1 / 1
1 |
biogrid
No evidence text available
26344197
bel
"However, mice homozygous for both UCH-L1 and UCH-L3 deletions die early due to dysphagia and display degeneration of the nucleus tractus solitarius and area postrema in addition to the degeneration of the gracile tract that is observed in GAD mice that only have a UCH-L1 deletion"
14556719
UCHL3 affects DNA Damage
| 1
UCHL3 activates DNA Damage. 1 / 1
| 1
reach
"UCHL3 enhanced the DNA damage repair mediated by radiotherapy and chemotherapy in cancer cells via binding to tyrosyl DNA phosphodiesterase 1 (TDP1), a chromosome breakage repair related enzyme, and mediating TDP1 deubiquitination [XREF_BIBR - XREF_BIBR]."
32351584
UCHL3 affects DLD
1 |
UCHL3 binds DLD. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects Caspase
| 1
UCHL3 inhibits Caspase. 1 / 1
| 1
reach
"Hence, loss of UCH-L3 leads to mitochondrial oxidative stress related photoreceptor cell apoptosis in a caspase independent manner."
16816367
reach
"UCHL3 promotes hepatocellular carcinoma cell migration by de-ubiquitinating and stabilizing Vimentin."
36969045
UCHL3 affects CSE1L
1 |
UCHL3 binds CSE1L. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects COPS6
1 |
UCHL3 binds COPS6. 1 / 1
1 |
biogrid
No evidence text available
19615732
UCHL3 affects CDH1
1 |
UCHL3 binds CDH1. 1 / 1
1 |
biogrid
No evidence text available
24798214
UCHL3 affects BTRC
| 1
BTRC binds UCHL3, COPS5, CUL1, and KITLG. 1 / 1
| 1
sparser
"Interestingly, we find that UCHL3 regulates COPS5-dependent deneddylation of Cullin1, which is an essential component of SCF β-TrCP complex and associated with SCF β-TrCP activities."
28583475
UCHL3 affects BMP
| 1
UCHL3 activates BMP. 1 / 1
| 1
eidos
"UCH-L3 can deubiquitinate and stabilize Smadl , and enhance BMP signaling ."
36340031
UCHL3 affects BAG3
1 |
UCHL3 binds BAG3. 1 / 1
1 |
biogrid
No evidence text available
23824909
UCHL3 affects AhR stability
| 1
UCHL3 activates AhR stability. 1 / 1
| 1
eidos
"UCHL3 increased AhR stability and the binding of AhR to the promoter regions of the `` stemness '' genes ATP-binding cassette subfamily G member 2 ( ABCG2 ) , KLF4 , and c-Myc ."
32546741
UCHL3 affects ATP6V1F
1 |
UCHL3 binds ATP6V1F. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects ARHGDIA
1 |
UCHL3 binds ARHGDIA. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 affects APP
| 1
UCHL3 inhibits APP. 1 / 1
| 1
reach
"This may be mechanistically attributed to the fact that loss of UCHL1 and UCHL3 accelerates the degradation of APP, thus accounting for the overproduction of Aβ (Zhang, Cai, Zhang, Zhang, & Song, 2014) (Table 2).2.5 Therapeutic implications."
29992725
UCHL3 affects AHCYL1
1 |
UCHL3 binds AHCYL1. 1 / 1
1 |
biogrid
No evidence text available
22939629
UCHL3 affects ACAA2
1 |
UCHL3 binds ACAA2. 1 / 1
1 |
biogrid
No evidence text available
26344197
UCHL3 inhibitors affects UCHL3
| 1
UCHL3 inhibitors inhibits UCHL3. 1 / 1
| 1
reach
"From the cancer point of view, UCHL3 is an attractive " druggable " target and previous efforts focused on developing UCHL3 inhibitors that specifically target UCHL3 in plasmodium falciparum parasite, offering promising selectivity due to structural differences compared to human UCHL3."
29898404
UCH Yuh1 affects UCHL3
| 1
UCHL3 binds UCH Yuh1. 1 / 1
| 1
reach
"Binding of ubiquitin leads to apparent stabilization of the crossover loop, as it was seen straddling the active site in co-crystal structures of ubiquitin aldehyde bound to UCHL3 (XREF_FIG and XREF_FIG) and the yeast UCH Yuh1 XREF_BIBR, XREF_BIBR."
27012468
UBXN7 affects UCHL3
1 |
UCHL3 binds UBXN7. 1 / 1
1 |
biogrid
No evidence text available
22939629
UBR1 affects UCHL3
1 |
UCHL3 binds UBR1. 1 / 1
1 |
biogrid
No evidence text available
22939629
UBQLN1 affects UCHL3
1 |
UBQLN1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
UBL7 affects UCHL3
1 |
UCHL3 binds UBL7. 1 / 1
1 |
biogrid
No evidence text available
22939629
UBE4B affects UCHL3
1 |
UBE4B binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
UBE2V1 affects UCHL3
1 |
UBE2V1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
UBE2S affects UCHL3
1 |
UCHL3 binds UBE2S. 1 / 1
1 |
biogrid
No evidence text available
20622874
UBE2L3 affects UCHL3
1 |
UBE2L3 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
UBE2A affects UCHL3
1 |
UBE2A binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
UBA1 affects UCHL3
1 |
UBA1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
Treatment PGJ cells affects UCHL3
| 1
Treatment PGJ cells inhibits UCHL3. 1 / 1
| 1
eidos
"Treatment with Delta 12 - PGJ 2 in cells also inhibited UCH-L1 and UCH-L3 without alteration of proteasomal activity , indicating that prostaglandins can be suitable for neural disorder therapy [ 95 ] ."
| PMC
Treatment Delta12-PGJ2 cells affects UCHL3
| 1
Treatment Delta12-PGJ2 cells inhibits UCHL3. 1 / 1
| 1
eidos
"Treatment with Delta12-PGJ2 in cells also inhibited UCH-L1 and UCH-L3 without alteration of proteasomal activity , indicating that prostaglandins can be suitable for neural disorder therapy [ 95 ] ."
| PMC
TTC9C affects UCHL3
1 |
UCHL3 binds TTC9C. 1 / 1
1 |
biogrid
No evidence text available
22939629
TTC1 affects UCHL3
1 |
TTC1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
TSN affects UCHL3
1 |
TSN binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
TRM1 affects UCHL3
1 |
UCHL3 binds TRM1. 1 / 1
1 |
virhostnet
No evidence text available
31873071
TRIM63 affects UCHL3
1 |
UCHL3 binds TRIM63. 1 / 1
1 |
biogrid
No evidence text available
22626734
TRIM55 affects UCHL3
1 |
UCHL3 binds TRIM55. 1 / 1
1 |
biogrid
No evidence text available
22626734
TRIM54 affects UCHL3
1 |
UCHL3 binds TRIM54. 1 / 1
1 |
biogrid
No evidence text available
22626734
TRIM27 affects UCHL3
1 |
UCHL3 binds TRIM27. 1 / 1
1 |
biogrid
No evidence text available
23105109
TPD52L1 affects UCHL3
1 |
TPD52L1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
TPD52 affects UCHL3
1 |
TPD52 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
TEAD4 affects UCHL3
| 1
TEAD4 increases the amount of UCHL3. 1 / 1
| 1
reach
"UCHL3 promoter analysis revealed that TEAD4, through which YAP bind to DNA, activated UCHL3 transcription by binding to the promoter of UCHL3."
36813921
TCF3 affects UCHL3
1 |
TCF3 decreases the amount of UCHL3. 1 / 1
1 |
biopax:msigdb
No evidence text available
TAGLN2 affects UCHL3
1 |
TAGLN2 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
TAGLN affects UCHL3
1 |
TAGLN binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
reach
"FACS analysis showed that inhibition of UCHL3 using TCID reduced CD338 expression and the percentage of SP cells among A549 cells overexpressing UCHL3 (Fig. 7e, f)."
32546741
Senkirkine affects UCHL3
1 |
Senkirkine increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
26100227
SUMO3 affects UCHL3
1 |
SUMO3 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
SUMO2 affects UCHL3
| 1
SUMO2 binds SUMO1 and UCHL3. 1 / 1
| 1
sparser
"In contrast, UCH-L3 did not bind to sentrin-1, sentrin-2, or sentrin-3."
9790970
SULT1A1 affects UCHL3
1 |
SULT1A1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
STIP1 affects UCHL3
1 |
STIP1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
SPOP affects UCHL3
| 1
SPOP activates UCHL3. 1 / 1
| 1
reach
"Interestingly, we found that the concomitant knockdown of SPOP with UCHL3 rescued the effect of UCHL3 knockdown on BRD4 protein (Fig. S4i), indicating that UCHL3 antagonizes SPOP to maintain BRD4 stability."
34290255
SOD2 affects UCHL3
1 |
SOD2 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
SNRPD1 affects UCHL3
1 |
SNRPD1 binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
22939629
SMS affects UCHL3
1 |
SMS binds UCHL3. 1 / 1
1 |
biogrid
No evidence text available
26344197
Rho-Ub-PA affects UCHL3
| 1
UCHL3 binds Rho-Ub-PA. 1 / 1
| 1
reach
"The activity of UCHL3 was also monitored using the fluorescent Ub-propargylamide (PA) DUB ABP Rho-Ub-PA.3 d Fluorescent bands corresponding to UCHL3 bound to Rho-Ub-PA were observed for both unlabeled UCHL3 and DTT treated Ub SS -UCHL3 complexes, but not for untreated Ub SS -UCHL3 complexes."
28841265
Radiation, Ionizing leads to the phosphorylation of UCHL3. 1 / 1
| 1
reach
"The phosphorylation of UCHL3 was also induced by IR treatment (data not shown)."
30559450
eidos
"Moreover , the suppression of UCHL3 by siRNA or the inhibition by TCID upregulated ubiquitinated Vimentin ."
36969045
RDX affects UCHL3
1 |
UCHL3 binds RDX. 1 / 1
1 |
biogrid
No evidence text available
26344197
Proteasome affects UCHL3
| 1
Proteasome activates UCHL3. 1 / 1
| 1
reach
"In addition, several ubiquitin-proteasome-related proteins (ubiquitin-activating enzyme E1, ubiquitin C-terminal hydrolase UCHL3 and proteasome 26S regulatory subunit) were crotonylated and 2-hydroxyisobutyrylated in the phenotypically different T. gondii parasites (supplemental Data S21)."
31488510
PRKDC affects Ku
| 1
UCHL3 binds PRKDC and Ku. 1 / 1
| 1
sparser
"These data thus suggested that UCHL3 interacts with Ku and/or DNA-PKcs in cells and could have a role at sites of DSBs in a catalytic-activity-dependent manner."
30559450
PR-619 affects UCHL3
| 1
PR-619 inhibits UCHL3. 1 / 1
| 1
reach
"Remarkably, only OTULIN binding was lost after treatment with the broad-spectrum DUB inhibitor PR-619, which also inhibits USP5 and UCHL3."
28919039
PPIB affects UCHL3
1 |
UCHL3 binds PPIB. 1 / 1
1 |
biogrid
No evidence text available
26344197
POGK affects UCHL3
1 |
UCHL3 binds POGK. 1 / 1
1 |
biogrid
No evidence text available
21900206
PLA2G2A affects UCHL3
1 |
UCHL3 binds PLA2G2A. 1 / 1
1 |
biogrid
No evidence text available
22118674
PKA affects UCHL3
| 1
PKA activates UCHL3. 1 / 1
| 1
reach
"UCH-L3, for example, is apparently stimulated by cAMP and PKA so as to enhance ENaC recycling."
20940433
PJA1 affects UCHL3
1 |
UCHL3 binds PJA1. 1 / 1
1 |
biogrid
No evidence text available
22626734
PGD affects UCHL3
1 |
UCHL3 binds PGD. 1 / 1
1 |
biogrid
No evidence text available
26344197
PGA1 affects UCHL3
| 1
PGA1 inhibits UCHL3. 1 / 1
| 1
reach
"Neither PGJ2 nor PGA1, D2, and E2 were found to inhibit UCH-L1 and UCH-L3."
15194490
PEBP1 affects UCHL3
1 |
UCHL3 binds PEBP1. 1 / 1
1 |
biogrid
No evidence text available
26344197
PDIA6 affects UCHL3
| 1
PDIA6 inhibits UCHL3. 1 / 1
| 1
reach
"However, it is also likely that the Arg at P5 in Ac-RLRGG-AFC negates the salt-dependent activation of UCH-L3, possibly because it is in contact with the mobile loop of the enzyme (Fig 3B) and thus serves a similar function to the salt effect."
18601651
PCMT1 affects UCHL3
1 |
UCHL3 binds PCMT1. 1 / 1
1 |
biogrid
No evidence text available
22939629
PBX1 affects UCHL3
1 |
UCHL3 binds PBX1. 1 / 1
1 |
biogrid
No evidence text available
22939629
P4HB affects UCHL3
1 |
UCHL3 binds P4HB. 1 / 1
1 |
biogrid
No evidence text available
26344197
OTULIN affects UCHL3
| 1
UCHL3 binds OTULIN. 1 / 1
| 1
reach
"His PD showed that OTULIN ABPDeltaG76 still bound to USP5 and UCHL3 (XREF_FIG I), confirming our MS results that these DUBs are associating largely in a non covalent manner with the probes (compare XREF_FIG B and 6C)."
28919039
NUP93 affects UCHL3
1 |
UCHL3 binds NUP93. 1 / 1
1 |
biogrid
No evidence text available
26344197
NT5DC1 affects UCHL3
1 |
UCHL3 binds NT5DC1. 1 / 1
1 |
biogrid
No evidence text available
22939629
NSG2 affects UCHL3
1 |
UCHL3 binds NSG2. 1 / 1
1 |
biogrid
No evidence text available
21900206
NRF1 affects UCHL3
1 |
NRF1 decreases the amount of UCHL3. 1 / 1
1 |
biopax:msigdb
No evidence text available
NEDD8 affects Ubiquitin
| 1
Ubiquitin binds UCHL3 and NEDD8. 1 / 1
| 1
sparser
"In vitro GST pull-down assay demonstrated that UCH-L3 bound to both NEDD8 and ubiquitin."
9790970
NEDD8 affects USP4
| 1
UCHL3 binds USP4 and NEDD8. 1 / 1
| 1
sparser
"NEDD8, UCHL3 and USP4 are highly associated with ubiquitination."
24093944
NEDD8 affects UCHL3, and Ubiquitin
| 1
Ubiquitin binds UCHL3 and NEDD8. 1 / 1
| 1
sparser
"In vitro GST pull-down assay demonstrated that UCH-L3 bound to both NEDD8 and ubiquitin."
9790970
NEDD8 affects UCHL3, and USP4
| 1
UCHL3 binds USP4 and NEDD8. 1 / 1
| 1
sparser
"NEDD8, UCHL3 and USP4 are highly associated with ubiquitination."
24093944
N-nitrosodiethylamine decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
20360939
N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-D-glucosaminyl group inhibits UCHL3. 1 / 1
| 1
sparser
"As LDN can also inhibit UCHL3, we determined the UCHL3 protein level in the sham group and MI group 14 days after operation and found that no significant differences between the two groups (Supplemental Fig.  xref )."
32606430
MSN affects UCHL3
1 |
UCHL3 binds MSN. 1 / 1
1 |
biogrid
No evidence text available
26344197
MMV676603 affects UCHL3
| 1
MMV676603 inhibits UCHL3. 1 / 1
| 1
reach
"Consequently, we can infer that MMV676603 inhibits PfUCHL3 activity by altering the key interaction of cysteine residue present in the active site pocket of the enzyme."
35042884
MAPRE1 affects UCHL3
1 |
UCHL3 binds MAPRE1. 1 / 1
1 |
biogrid
No evidence text available
26344197
LONRF1 affects UCHL3
1 |
UCHL3 binds LONRF1. 1 / 1
1 |
biogrid
No evidence text available
23105109
LMO7 affects Ubiquitin
| 1
Ubiquitin binds UCHL3 and LMO7. 1 / 1
| 1
sparser
"We suggest that the increase in severity may reflect an interaction between Uchl3 and Lmo7 in the ubiquitin-mediated protein degradation pathway."
12761045
LINC00665 affects UCHL3
| 1
LINC00665 increases the amount of UCHL3. 1 / 1
| 1
reach
"LINC00665 functioned as a sponge of miR-582-5p and thus up-regulated the expression of the miR-582-5p target UCHL3."
35918714
LEF1 affects UCHL3
1 |
LEF1 decreases the amount of UCHL3. 1 / 1
1 |
biopax:msigdb
No evidence text available
LDN affects UCHL3
| 1
LDN inhibits UCHL3. 1 / 1
| 1
reach
"As LDN can also inhibit UCHL3, we determined the UCHL3 protein level in the sham group and MI group 14days after operation and found that no significant differences between the two groups (Supplemental Fig. 1)."
32606430
LDN 57444 affects UCHL3
| 1
LDN 57444 inhibits UCHL3. 1 / 1
| 1
eidos
"LDN57444 also inhibit UCHL3 activity ( IC50 = 25 muM ) ( 39 ) , whereas , 6RK73 showed almost no inhibition of UCHL3 ( IC50 = 236 muM ) ( Fig. 5B ) ."
31857432
Ku affects PRKDC, and UCHL3
| 1
UCHL3 binds PRKDC and Ku. 1 / 1
| 1
sparser
"These data thus suggested that UCHL3 interacts with Ku and/or DNA-PKcs in cells and could have a role at sites of DSBs in a catalytic-activity-dependent manner."
30559450
KITLG affects UCHL3
| 1
BTRC binds UCHL3, COPS5, CUL1, and KITLG. 1 / 1
| 1
sparser
"Interestingly, we find that UCHL3 regulates COPS5-dependent deneddylation of Cullin1, which is an essential component of SCF β-TrCP complex and associated with SCF β-TrCP activities."
28583475
KEAP1 affects UCHL3
| 1
UCHL3 binds KEAP1 and BAP1. 1 / 1
| 1
sparser
"To examine whether BAP1 and UCHL3 directly interacts with KEAP1, we cotransfected pcDNA3-FLAG-BAP1 or pcDNA3-FLAG-UCHL3 with pcDNA3-HA-KEAP1 in 293T cells and immunoprecipitated cell lysates with FLAG-agarose beads followed by Western blot analysis with HA-HRP antibody."
35052618
K27 affects UCHL3
| 1
K27 inhibits UCHL3. 1 / 1
| 1
eidos
"Using our Ub tools to profile UCHL3 's activity , we generate a quantitative kinetic model of the inhibitory mechanism and we find that K27 Ub 2 can inhibit UCHL3 covalently , by binding to its catalytic cysteine , and allosterically , by locking its catalytic loop tightly in place ."
33238157
ISYNA1 affects UCHL3
1 |
UCHL3 binds ISYNA1. 1 / 1
1 |
biogrid
No evidence text available
26344197
INS affects UCHL3
| 1
INS inhibits UCHL3. 1 / 1
| 1
reach
"The phosphorylation of insulin and IGF-I receptor, Akt, glycogen synthase kinase-3beta, and FoxO1 was decreased in Uchl3 (-/-) MEFs treated with insulin."
19837878
IMPDH2 affects UCHL3
1 |
UCHL3 binds IMPDH2. 1 / 1
1 |
biogrid
No evidence text available
26344197
ICG 001 affects UCHL3
1 |
ICG 001 decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
26191083
HSD17B10 affects UCHL3
1 |
UCHL3 binds HSD17B10. 1 / 1
1 |
biogrid
No evidence text available
26344197
HINT1 affects UCHL3
1 |
UCHL3 binds HINT1. 1 / 1
1 |
biogrid
No evidence text available
26344197
HELLS affects UCHL3
| 1
UCHL3 binds HELLS. 1 / 1
| 1
reach
"At the same time, the lncRNA GIAT4RA counteracts the deubiquitinating effects of LSH by interfering with interaction between LSH and UCH-L3, reducing levels of LSH protein, and playing the role in inhibiting the invasion and migration of LUAD cells83."
34405018
HA-Ub affects UCHL3
| 1
UCHL3 binds HA-Ub. 1 / 1
| 1
reach
"The blot shows full formation of the HA-Ub and UCHL3 complex at all concentrations after preincubation with 1 indicating the molecule does not inhibit UCHL3 in cells (XREF_FIG B, second panel)."
33668938
GST affects UCHL3
| 1
GST binds UCHL3. 1 / 1
| 1
sparser
"We observed a reduced ubiquitin signal with GST-UCHL3 but not with GST, suggesting that UCHL3 directly removed ubiquitin from Ku80."
30559450
GSK-J4 affects UCHL3
1 |
GSK-J4 increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
29301935
GRL0617 affects UCHL3
| 1
GRL0617 inhibits UCHL3. 1 / 1
| 1
reach
"Importantly, GRL0617 was unable to inhibit HAUSP, USP18, UCH-L1, UCH-L3, and a papain-like protease (PLP2) from the human coronavirus NL63."
35186898
GPX4 affects UCHL3
1 |
UCHL3 binds GPX4. 1 / 1
1 |
biogrid
No evidence text available
26344197
GDI1 affects UCHL3
1 |
UCHL3 binds GDI1. 1 / 1
1 |
biogrid
No evidence text available
26344197
FlaG affects BAP1
| 1
UCHL3 binds BAP1 and FlaG. 1 / 1
| 1
sparser
"We observed that FLAG-BAP1 ( xref B, upper panel), but not FLAG-UCHL3 ( xref B, lower panel), directly binds to HA-KEAP1."
35052618
FOXJ2 affects UCHL3
1 |
FOXJ2 decreases the amount of UCHL3. 1 / 1
1 |
biopax:msigdb
No evidence text available
FH affects UCHL3
| 1
UCHL3 binds FH. 1 / 1
| 1
sparser
"The data showed the interaction between FH-UCHL3 and endogenous COPS5 ( Fig. 4 A)."
28583475
Environmental Pollutants increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
20064776
EZR affects UCHL3
1 |
UCHL3 binds EZR. 1 / 1
1 |
biogrid
No evidence text available
26344197
ENO2 affects UCHL3
1 |
UCHL3 binds ENO2. 1 / 1
1 |
biogrid
No evidence text available
26344197
ENO1 affects UCHL3
1 |
UCHL3 binds ENO1. 1 / 1
1 |
biogrid
No evidence text available
26344197
EGR4 affects UCHL3
1 |
EGR4 decreases the amount of UCHL3. 1 / 1
1 |
biopax:msigdb
No evidence text available
EGR1 affects UCHL3
1 |
EGR1 decreases the amount of UCHL3. 1 / 1
1 |
biopax:msigdb
No evidence text available
E2 affects UCHL3
| 1
E2 inhibits UCHL3. 1 / 1
| 1
reach
"Neither PGJ2 nor PGA1, D2, and E2 were found to inhibit UCH-L1 and UCH-L3."
15194490
DUB inhibitor affects UCHL3
| 1
DUB inhibitor activates UCHL3. 1 / 1
| 1
reach
"b-AP15 is not an indiscriminate DUB inhibitor, since in isolated DUB assays it does not inhibit USP2/7/8, UCHL1, UCHL3 or BAP1 at 50 μM. Even though 19S inhibitory potency was modest, the Linder group showed that 37 inhibited tumour progression in four different in vivo solid tumour models in mice (2.5-5 mg/kg by subcutaneous injection), validating the 19S regulatory particle as a new anticancer drug target."
26852935
DRB affects UCHL3
| 1
DRB inhibits UCHL3. 1 / 1
| 1
reach
"Consistent with a predominant role of TDP1 during transcription, inhibition of transcription using 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) suppressed the turnover rate of TDP1 (XREF_FIG F) and abrogated the UCHL3 dependent difference in TOP1-SSBs (XREF_FIG G)."
29898404
DNA UCHL3 phosphorylation affects UCHL3
| 1
DNA UCHL3 phosphorylation activates UCHL3. 1 / 1
| 1
eidos
"Lastly , we provide evidence showing that DNA damage-induced UCHL3 phosphorylation ( which is dependent on UCHL3 catalytic activity , ATM kinase and downstream NHEJ factors ) promotes destabilization of UCHL3 , suggesting that UCHL3 may dissociate from Ku80 to allow Ku ubiquitylation and subsequent removal from DNA ."
30559450
DLD affects UCHL3
1 |
UCHL3 binds DLD. 1 / 1
1 |
biogrid
No evidence text available
26344197
DDT affects UCHL3
1 |
DDT increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
30207508
CUL1 affects UCHL3
| 1
BTRC binds UCHL3, COPS5, CUL1, and KITLG. 1 / 1
| 1
sparser
"Interestingly, we find that UCHL3 regulates COPS5-dependent deneddylation of Cullin1, which is an essential component of SCF β-TrCP complex and associated with SCF β-TrCP activities."
28583475
CSE1L affects UCHL3
1 |
UCHL3 binds CSE1L. 1 / 1
1 |
biogrid
No evidence text available
22939629
CRY2 silencing affects UCHL3
| 1
CRY2 silencing inhibits UCHL3. 1 / 1
| 1
eidos
"Moreover , CRY2 silencing abolished UCHL3 deficiency-mediated inhibition in cell growth and migration ."
35088238
COPS6 affects UCHL3
1 |
UCHL3 binds COPS6. 1 / 1
1 |
biogrid
No evidence text available
19615732
COPS5 affects KITLG
| 1
BTRC binds UCHL3, COPS5, CUL1, and KITLG. 1 / 1
| 1
sparser
"Interestingly, we find that UCHL3 regulates COPS5-dependent deneddylation of Cullin1, which is an essential component of SCF β-TrCP complex and associated with SCF β-TrCP activities."
28583475
CANT1 affects UCHL3
| 1
CANT1 inhibits UCHL3. 1 / 1
| 1
reach
"It appears therefore that SCAN1 cells downregulate UCHL3 in an attempt to avoid the unfavorable outcomes of accumulating further PDBs."
29898404
C646 compound affects UCHL3
1 |
C646 compound decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
26191083
BTRC affects UCHL3
| 1
BTRC binds UCHL3, COPS5, CUL1, and KITLG. 1 / 1
| 1
sparser
"Interestingly, we find that UCHL3 regulates COPS5-dependent deneddylation of Cullin1, which is an essential component of SCF β-TrCP complex and associated with SCF β-TrCP activities."
28583475
BAP1 affects KEAP1, and UCHL3
| 1
UCHL3 binds KEAP1 and BAP1. 1 / 1
| 1
sparser
"To examine whether BAP1 and UCHL3 directly interacts with KEAP1, we cotransfected pcDNA3-FLAG-BAP1 or pcDNA3-FLAG-UCHL3 with pcDNA3-HA-KEAP1 in 293T cells and immunoprecipitated cell lysates with FLAG-agarose beads followed by Western blot analysis with HA-HRP antibody."
35052618
BAP1 affects FlaG, and UCHL3
| 1
UCHL3 binds BAP1 and FlaG. 1 / 1
| 1
sparser
"We observed that FLAG-BAP1 ( xref B, upper panel), but not FLAG-UCHL3 ( xref B, lower panel), directly binds to HA-KEAP1."
35052618
BAG3 affects UCHL3
1 |
UCHL3 binds BAG3. 1 / 1
1 |
biogrid
No evidence text available
23824909
Akt inhibitor affects UCHL3
| 1
Akt inhibitor activates UCHL3. 1 / 1
| 1
reach
"Here we report that perifosine, a previously reported Akt inhibitor, can inhibit UCHL3 in vitro and in vivo."
31113933
Ac-RLRGG-AFC affects UCHL3
| 1
Ac-RLRGG-AFC inhibits UCHL3. 1 / 1
| 1
reach
"However, it is also likely that the Arg at P5 in Ac-RLRGG-AFC negates the salt-dependent activation of UCH-L3, possibly because it is in contact with the mobile loop of the enzyme (Fig 3B) and thus serves a similar function to the salt effect."
18601651
ATP6V1F affects UCHL3
1 |
UCHL3 binds ATP6V1F. 1 / 1
1 |
biogrid
No evidence text available
22939629
ARHGDIA affects UCHL3
1 |
UCHL3 binds ARHGDIA. 1 / 1
1 |
biogrid
No evidence text available
26344197
AHCYL1 affects UCHL3
1 |
UCHL3 binds AHCYL1. 1 / 1
1 |
biogrid
No evidence text available
22939629
ACAA2 affects UCHL3
1 |
UCHL3 binds ACAA2. 1 / 1
1 |
biogrid
No evidence text available
26344197
2-hydroxypropanoic acid decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
30851411
sparser
"Treatment with Δ 12 -PGJ 2 in cells also inhibited UCH-L1 and UCH-L3 without alteration of proteasomal activity, indicating that prostaglandins can be suitable for neural disorder therapy [ 95 ] ."
| PMC
1H-pyrazole affects UCHL3
1 |
1H-pyrazole increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
17945193
15Delta-PGJ2 affects UCHL3
| 1
15Delta-PGJ2 inhibits UCHL3. 1 / 1
| 1
reach
"Delta12-PGJ2 and 15Delta-PGJ2 are potent cyclopentone PGs and have been reported to inhibit UCH-L3 and UCH-L1 respectively [282,283]."
28923280
15-deoxy-Delta12 affects UCHL3
| 1
15-deoxy-Delta12 inhibits UCHL3. 1 / 1
| 1
eidos
"The inhibitory effect of prostaglandins is exemplified by 15-deoxy-Delta12 ,14 - prostaglandin J2 ( 15d-PGJ2 ) ( Fig. 2 , compound 4 ) , which inhibits the activities of UCHL-3 ( Ref ."
22088887
15-deoxy-Delta(12,14)-prostaglandin J2 inhibits UCHL3. 1 / 1
| 1
reach
"The inhibitory effect of prostaglandins is exemplified by 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) (Fig. 2, compound 4), which inhibits the activities of UCHL-3 (Ref."
22088887
1,2-dichloroethane decreases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
28960355
4,4'-sulfonyldiphenol increases the amount of UCHL3. 1 / 1
1 |
ctd
No evidence text available
27685785
3',5'-cyclic AMP affects UCHL3
| 1
reach
"UCH-L3, for example, is apparently stimulated by cAMP and PKA so as to enhance ENaC recycling."
20940433