IndraLab

Statements


COPS6 affects COPS5
23 3 | 30 73
COPS6 binds COPS5.
23 3 | 27 72
23 3 | 25 66

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"Molecular dynamics simulation studies further suggest that heterodimerization of CSN5 with CSN6 facilitates inhibitor binding by stabilizing an open ins-1 loop conformation [ 58 ]."

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"CSN5 and CSN6, which have an MPN (Mpr1 and Pad1 N-terminal) domain, form a dimer and are embedded at the core of the helical bundle."

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"The structure reaffirms the conserved activation mechanism of the deneddylation machinery, including conformational clamping of CRL2 by CSN2/CSN4, release of the catalytic CSN5/CSN6 heterodimer, and s[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"The above suggests that the MPN dimer (CSN5CSN6 dimer) could be highly conserved."

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sparser
"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain and the dimer is topologically knotted to engage in Cullin deneddylation xref ."

sparser
"MD simulations studies and binding energy analysis revealed that the selective binding of the inhibitors can be only explained by the consideration of larger heterodimeric complexes of Rpn11 (Rpn8-Rpn11) and CSN5 (CSN5-CSN6)."

sparser
"In the crystal structure of CSN5-CSN6 heterodimer, initial Ins-1 loop (98–109) conformation blocks the distal ubiquitin binding site which we also observed in cryo-EM structure of Rpn11."
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sparser
"CSN5 alone is inactive due to an auto-inhibited conformation of its catalytic domain, and the association of CSN5 with the CSN6 MPN (Mpr1/Pad1 N-terminal) domain activates its isopeptidase activity xref ."

sparser
"The association of CSN5 and CSN6 MPN (for Mpr1/Pad1 N-terminal) domains activates its isopeptidase activity."

sparser
"Here we report that CSN6 N-terminal domain containing its MPN domain can form a stable heterodimer with CSN5 catalytic domain in vitro ."
CSN3 binds COPS5 and COPS6. 3 / 3
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sparser
"Our data also demonstrate that CSNAP binds to CSN3, CSN5 and CSN6, possibly linking the MPN and PCI substructures."

sparser
"We show that CSNAP binds CSN3, CSN5 and CSN6 and its incorporation into the CSN complex is mediated through the C-terminal region involving conserved aromatic residues."

sparser
"CSNAP interacts with CSN3, CSN5, and CSN6."
COPS6 binds COPS5 and MPN domain. 2 / 2
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"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain, and the dimer is topologically knotted to engage in Cullin deneddylation."

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"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain and the dimer is topologically knotted to engage in Cullin deneddylation 55."
COPS6 activates COPS5.
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COPS6 activates COPS5. 2 / 3
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"Previous study has shown that over-expression of CSN6 increases its competition with CSN5 in terms of cullin binding, and thereby inhibits accessibility of cullin to CSN5 for deneddylation, which stab[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"To gain additional insights into the molecular mechanism of CSN5 activation by CSN6, the crystal structure of the MPN − core fragment was determined by molecular replacement at 1.76 Å resolution, using the human Rpn8 ΔC orthologue as the search model (PDB code 2O95; xref )."
COPS6 inhibits COPS5.
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COPS6 inhibits COPS5. 2 / 2
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"Previous study has shown that over-expression of CSN6 increases its competition with CSN5 in terms of cullin binding, and thereby inhibits accessibility of cullin to CSN5 for deneddylation, which stab[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Notably, the MPN domain of CSN6 inhibited the association of CSN5 with Cullin-1, while the C-terminal domain had less impact (XREF_FIG)."
COPS6 affects COPS4
21 4 | 1 11
21 4 | 1 10

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sparser
"The key structural rearrangements include: (i) the presence of a neddylated SCF triggers the convergence of the N-terminal helical arms of CSN2 and CSN4 (hereafter CSN2 arm and CSN4 arm respectively), clamping CUL1 CTD and RBX1 RING , (ii) the flexible CUL1 WHB is repositioned against the stabilised RBX1 RING , aligning NEDD8 at the CSN active site and (iii) concurrent disruption of the CSN4-CSN6 interface enables flexibility and remodelling of the CSN5-CSN6 MPN domains, bringing the CSN5 active site closer to NEDD8 (Fig.  xref )."

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sparser
"The core of the subcomplex is based on a stable heterotrimeric association of CSN7, CSN4, and CSN6, requiring coexpression in a bacterial reconstitution system."
COPS6 affects CDKN2A
2 | 29 4
COPS6 inhibits CDKN2A.
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"Taken together, the above data demonstrated that CSN6 downregulated p16 stability through regulating the 20S proteasome ubiquitin-independent degradation pathway."

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"CSN6 promoted p16 degradation in gastric cancer via ubiquitination 15."

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"Our mechanistic studies indicated that CSN6 interacted with p16, and that CSN6-mediated p16 downregulation was suppressed by a proteasome inhibitor."

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"In the current study, we examined the mechanism underlying the CSN6-mediated p16 degradation."

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"Furthermore, CSN6 decreased the stability of tumor suppressor p16 in an ubiquitin-independent proteasomal degradation pathway and thereby promoted tumorigenicity (Figure 7)."

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"To explore whether the 20S proteasome activator REGγ was involved in CSN6 induced p16 degradation, we performed co-IP to examine the interaction of the proteins."

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"It is our goal to confirm whether CSN6 promotes the degradation of p16 via an ubiquitination pathway."

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"CSN6 downregulates p16 via proteasomal degradation ."

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"The mechanism underlying CSN6-mediated p16 degradation in GC remains to be explored."

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"Thus, we may reach a conclusion that CSN6 promotes p16 degradation through an ubiquitin-independent pathway."
COPS6 binds CDKN2A.
2 | 2 3
2 | 2 3

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sparser
"Our study provides the in vitro and in vivo evidence that CSN6-p16 serves as an important target for GC therapy."

sparser
"Immunofluorescence localization and a co-immunoprecipitation study were used to show the interaction between the protein CSN6 and p16."

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"Immunofluorescence localization and a co-immunoprecipitation study were used to show the interaction between the protein CSN6 and p16."

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"In addition, co-IP showed association between endogenous CSN6 and p16 in MKN-45 and MGC-803 cells."

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sparser
"Our mechanistic studies indicated that CSN6 interacted with p16, and that CSN6-mediated p16 downregulation was suppressed by a proteasome inhibitor."
COPS6 decreases the amount of CDKN2A.
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COPS6 decreases the amount of CDKN2A. 4 / 4
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"We then examined whether CSN6 reduced the expression of p16 through E3 ubiquitin ligases."

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"Thus, these observations present a paradox: How does CSN6 reduce p16 expression?"

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"CSN6 promoted the loss of p16-mediated tumor progression and played an important role in regulating ubiquitin-independent proteasomal degradation of p16."

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"Although the results showed that there was no significant change in the p21 in both cell lines (Figure 3A), it was shown that CSN6 could downregulate the steady-state expression of endogenous p16 in a dose-dependent manner in GC cells (Figure 3B)."
COPS6 increases the amount of CDKN2A.
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COPS6 increases the amount of CDKN2A. 3 / 3
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"Moreover, the expression trends of CSN6 and p16 in CSN6-treated tumor groups were consistent with the results of in vitro studies, which demonstrated that CSN6 promoted cancer cell growth by reducing p16 expression."

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"Thus, we performed ubiquitination assay and found that CSN6 overexpression did not increase the endogenous ubiquitination level of p16 (Figure 5A)."

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"Additionally, our data demonstrated that CSN6 promoted proliferation of GC by inhibiting the expression of p16 via an ubiquitination-independent degradation pathway."
COPS6 ubiquitinates CDKN2A.
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COPS6 leads to the ubiquitination of CDKN2A. 2 / 2
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sparser
"However, our study showed that CSN6 did not promote the p16 ubiquitination during the degradation process."

reach
"However, our study showed that CSN6 did not promote the p16 ubiquitination during the degradation process."
COPS6 activates CDKN2A.
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COPS6 activates CDKN2A. 1 / 1
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"Furthermore, the decrease of REGγ could rescue CSN6-mediated downregulation of p16."
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"As shown in Fig. 3 , overexpression of FOXA1 could enhance CFPAC-1 cells’ invasion and metastasis abilities, and the re-expression of FOXA1 could attenuate the decrease of invasion and metastasis indu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Also, WT CSN6 increased cell migration and invasion in a wound healing assay (p < 0.001, XREF_FIG) and in transwell migration and invasion assays (XREF_FIG), whereas CSN6 S148A lost such capability, demonstrating that the CSN6 promoted migration and invasion of colon cancer cells requires a phosphorylation event."

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"Our results showed that knocking down FOXA1 inhibited the increase of invasion and metastasis abilities stimulated by overexpression of CSN6 ( Fig. 3 g-j)."

eidos
"In summary , these findings demonstrated that downregulation of CSN6 expression could inhibit cell proliferation , migration and invasion ."

eidos
"CSN6 promotes GBM proliferation , migration , invasion , and tumorigenesis through upregulation of EGFR by blocking its ubiquitination ; this happens as a result of interactions with CHIP that cause its degradation , although CHIP auto-ubiquitination occurs through an unknown mechanism [ 91 ] ."

eidos
"Here , we identified that CSN6 promoted melanoma cell migration and invasion in vivo ."

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"CSN6 promotes the migration and invasion of CC cells through inhibiting autophagy."

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"Both COPS6 and COPS9 overexpression promoted HCC cell migration and Matrigel invasion in transwell assays, while COPS6 siRNA transfection or COPS9 siRNA transfection repressed transwell migration and Matrigel invasion (Figure 11C)."

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"Furthermore, migration and invasion assays showed that knockdown of CSN6 reduced cell migration and invasion in DLD-1."

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"Overexpression of COPS6 or COPS9 increased HCC cell proliferation, migration, and invasion while knockdown suppressed these pro-tumorigenic and metastatic properties."

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"COPS6 promotes tumor progression and reduces CD8+ T cell infiltration by repressing IL-6 production to facilitate tumor immune evasion in breast cancer."

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"COPS6, a member of the JAMM family, has recently been verified to facilitate tumor advancement and decrease the infiltration of CD8 T-cells by suppressing the synthesis of IL-6, thus promoting tumor immune escape from cancer [44, 45]."

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"Furthermore, COPS6 was found to inhibit CD8+ T-cell infiltration in the tumor microenvironment, which facilitates tumor immune escape."

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"By regulating IL-6 secretion, p53/COPS6 reduces the infiltration and function of CD8 T cells, thus promoting the progression of breast cancer (27)."

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"This aligns with Du et al[28]’s results, demonstrating that COPS6 could inhibit CD8+ T cell infiltration within the tumor microenvironment (TME), thereby facilitating tumor immune evasion."

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"In summary, these findings demonstrated that downregulation of CSN6 expression could inhibit cell proliferation, migration and invasion."

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"In summary, our study showed that down- regulation of CSN6 inhibits invasion via autophagic degradation of CTSL in CC."

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"To assess the metastatic effects of CSN6 in CC, we performed transwell assay and found that down-regulation of CSN6 strongly decreased the migration and invasion abilities of CC cells (Fig. 2H)."

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"In human breast cancer MCF-7 cells, COPS6 overexpression stimulated p-AKT expression as well as the proliferation and malignant transformation of tumor cells, whereas knockdown of COPS6 caused opposite effects."

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"In addition, studies shown that CSN6 has the function of deubiquitinase and promoted tumorigenesis, proliferation, metastasis and EMT by inhibiting substrates degradation [ 14–16 ]."

eidos
"In summary , these findings demonstrated that downregulation of CSN6 expression could inhibit cell proliferation , migration and invasion ."

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"Our results demonstrated that CSN6 promoted the growth and proliferation capacities of cancer cells by G1 arrest."

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"Fig. 2 B shows that PDGF increased the protein level of CSN6 in a time-dependent manner, and peak time for 10 ng/ml PDGF up-regulation of CSN6 was 10 min, suggesting that CSN6 is up-regulated in PASMC[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Overexpression of COPS6 or COPS9 increased HCC cell proliferation, migration, and invasion while knockdown suppressed these pro-tumorigenic and metastatic properties."

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"Through combinatorial effects on the MDM2-p53 signaling axis and now SKP2 mediated p57 Kip2 degradation, it is hypothesized that CSN6 overexpression can effectively promote cell proliferation by simultaneously relieving cell cycle restraints, apoptosis, senescence, and presumably p53 functions in DNA damage repair and cell metabolism, among other important tumor suppressive mechanisms."

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"In melanoma cells, CSN6 knockdown remarkably inhibited cell proliferation, tumorigenicity, migration, and invasion, whereas CSN6 recovery rescued the proliferative and metastatic abilities."

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"Furthermore, in CSN6-knockdown melanoma cells, UBR5 knockdown abrogated the effects caused by CSN6 silencing, suggesting that CSN6 activates the UBR5 and CDK9 pathway to promote melanoma cell proliferation and metastasis."

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"To investigate the cell viability of CSN6-knockdown melanoma cells, a 3-[4, -5-dimethylthiazol-2-yl]-2, -5-diphenyltetrazolium bromide (MTT) assay was conducted and revealed that CSN6 knockdown remarkably inhibited melanoma cell proliferation in vitro."

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"In summary, these findings demonstrated that downregulation of CSN6 expression could inhibit cell proliferation, migration and invasion."

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"Because 14-3-3sigma suppresses CSN6 mediated cell proliferation and anchorage independent growth, we next examined the impact of 14-3-3sigma on CSN6 mediated tumor promotion."
COPS6 affects FOXO4
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COPS6 binds FOXO4.
2 | 1 9
2 | 1 9

sparser
"Results showed that FOXO4 bound to the C‐terminus of CSN6 (aa 185‐327 containing) but not to the N‐terminus (aa 1‐184, containing the MPN domain; Figure  xref )."

sparser
"Significantly, as the CSN6FOXO4 axis is crucial in regulating gene expression of Glut1 and SGOC genes, these genes were reduced in CSN6 knockdown tumors (Figure S17E, Supporting Information)."

sparser
"Together, the regulatory circuit of CSN6FOXO4 axis could be recapitulated in mouse xenograft cancer model, and level of FOXO4 deregulation plays roles in affecting the outcome of tumorigenicity."

sparser
"These data suggested that CSN6FOXO4 axis deregulation could exist in many types of cancer."

sparser
"CSN6FOXO4 Axis Is Critical for Tumorigenicity in Xenograft Mouse Model."

sparser
"Deregulation of CSN6FOXO4 Axis Rewires Metabolic Programming via Enhancing Glucose Uptake and Promotes the Expression of SGOC Genes."

sparser
"We further confirmed that CSN6FOXO4 link could affect cell proliferation."

sparser
"As CSN6FOXO4 axis impacts on the expression of Glut1, biochemical assays that quantitates the glucose uptake (consumption) by assessing uptake of (2‐( N ‐(7‐nitrobenz‐2‐oxa‐1,3‐diazol‐4‐yl)amino)‐2‐deoxyglucose (2‐NBDG)), a green fluorescent glucose analog, additionally demonstrated that CSN6 knockdown inhibited 2‐NBDG uptake (Figure  xref ), while FOXO4 knockdown increased 2‐NBDG uptake (Figure  xref )."

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"Mechanistic studies show that CSN6 binds and regulates FOXO4 stability through enhancing the E3 ligase activity of COP1, and that COP1 directly interacts with FOXO4 through a VP motif on FOXO4 and accelerates the ubiquitin-mediated degradation of FOXO4."

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COPS6 inhibits FOXO4.
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COPS6 inhibits FOXO4. 9 / 9
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"We have shown that CSN6 decreases FOXO4 stability."

eidos
"As CSN6 mitigates the expression level of FOXO4 , we showed that CSN6 overexpression leads to increased gene expression of Glut1 ( Figure 5F , Figure S15A , Supporting Information ) ."

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"Since MDM2 was shown as an E3 ligase for FOXO4 proteins, 33 , 34 we examined whether MDM2 is involved in CSN6‐mediated FOXO4 degradation."

eidos
"We further investigate how CSN6 downregulates FOXO4 ."

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"2.1 EGF Signal and CSN6/COP1 Enhance Ubiquitin-Mediated Degradation of FOXO4."

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"39 Our studies show that constitutively active mutant FOXO4A3, 41 an PKB/Akt phosphorylation mutant of FOXO4, is still sensitive to CSN6/COP1‐mediated degradation, suggesting that CSN6/COP1‐mediated FOXO4 degradation is not similar to the action mode of Skp2 or MDM2."

eidos
"] Our data indicates that CSN6 enhances ubiquitin-mediated degradation of FOXO4 through K48 link ubiquitination , thereby downregulating FOXO4 ."

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"Importantly, EGF‐mediated down regulation of FOXO4 can be reversed by the knockdown of CSN6 (Figure 1D), suggesting the involvement of CSN6 during this process."

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"We further investigate how CSN6 downregulates FOXO4."
COPS6 decreases the amount of FOXO4.
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COPS6 decreases the amount of FOXO4. 3 / 3
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"In contrast, protein analysis demonstrated that CSN6 knockdown reduced the expression of Glut1 while increased the expression of FOXO4 (Figure 5F)."

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"In line with this finding, Western blotting showed that knockdown of CSN6 by shRNA increased the steady‐state expression of FOXO4 (Figure 1F)."

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"We then found that CSN6 decreased the steady‐state expression of FOXO4 in a dose‐dependent manner in several CRC cell lines (Figure 1E, Figure S2A, Supporting Information)."
COPS6 ubiquitinates FOXO4.
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COPS6 leads to the ubiquitination of FOXO4. 2 / 2
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"As expected, wt FOXO4 ubiquitination was enhanced by CSN6."

sparser
"As expected, wt FOXO4 ubiquitination was enhanced by CSN6."
COPS6 activates FOXO4.
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COPS6 activates FOXO4. 2 / 2
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"Interestingly, wt CSN6 increased the turnover rate of FOXO4 to destabilize FOXO4 in a dose‐dependent manner, while CSN6 S60A had lost such a capability (Figure 7C)."

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"CSN6 overexpression increased the turnover rate of FOXO4 (Figure 2C, Figure S5C, Supporting Information)."
COPS6 increases the amount of FOXO4.
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COPS6 increases the amount of FOXO4. 1 / 1
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"We then found that CSN6 knockdown reduced the ubiquitination level of FOXO4 (Figure 2E), whereas overexpression of CSN6 increased the ubiquitination level of FOXO4 in a dose‐dependent manner (Figure 2E)."
COPS6 is modified
| 1 18 9
COPS6 is phosphorylated. 10 / 16
| 1 10 5

rlimsp
"PI3K/Akt inhibitor L294002 reduced the S60 phosphorylation level of CSN6 and caused subsequent E6AP downregulation (Figure 6B)."

rlimsp
"In addition, phosphorylation of CSN6 increases the stability of CSN6, thereby promoting its regulatory capacity."

sparser
"In addition, phosphorylation of CSN6 increases the stability of CSN6, thereby promoting its regulatory capacity."

reach
"These data imply that CSN6 possibly regulates the gene expression of SGOC genes and impacts SGOC amino acid metabolism through its negative impact on FOXO4 that transcriptionally suppresses the gene expression of SGOC genes.2.6 PKB/Akt-Mediated CSN6 Phosphorylation Enhances COP1-Regulated FOXO4 Ubiquitination and Subsequent Elevation of SGOC Genes."

sparser
"To determine whether the PKB/Akt‐mediated CSN6 phosphorylation affects FOXO4 stability, we examined the steady‐state expression of FOXO4 and COP1 in the presence of CSN6S60A, a construct with no PKB/Akt phosphorylation site."

sparser
"Treatment with calf intestinal alkaline phosphatase (CIP) reduced the EGF-induced steady-state expression of CSN6, indicating that CSN6 phosphorylation is required in this process ( xref ) and presumably acts through ERK."

sparser
"To confirm the existence of an ERK-specific phosphorylation site in CSN6 in vivo, we used an anti–phospho-serine antibody to detect the increase of serine-phosphorylated CSN6 in EGF-treated cells ( xref )."

sparser
"MEK1 inhibition with PD98059 treatment abolished the serine phosphorylation of CSN6 ( xref )."

sparser
"These results indicate that PKB/Akt‐mediated CSN6 phosphorylation is critical in stabilizing COP1, thereby enhancing ubiquitination level of FOXO4."

sparser
"Together, these results indicate that EGF/ERK mediated CSN6 phosphorylation and diminished CSN6 ubiquitination and turnover."
COPS6 is phosphorylated on S60. 7 / 7
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sparser
"S60 of CSN6 is phosphorylated by PKB/Akt [ xref ] and is critical for CSN6 stabilization."

rlimsp
"CSN6 S60 phosphorylation is known to increase the stability of CSN6 [21]."

sparser
"First, we have shown that S60 of CSN6 can be phosphorylated by PKB/Akt [ xref ] and that PKB/Akt enhances the steady‐state expression of CSN6. [ xref ] This S60 site is located in CSN6's MPN ( M pr1p and P ad1p N ‐terminal) domain, a domain found in the N‐terminus of yeast Mpr1 and Pad1 proteins. [ xref , xref , xref ] MPN domain contains polar residues that resemble the active site residues of metalloproteases [ xref ] and is involved in a proteasome‐associated deneddylation activity. [ xref ] Also the MPN domain is involved in heterodimerization between CSN6 and CSN5 to regulate Cullin neddylation. [ xref ] How S60 phosphorylation participates in any activity of the MPN domain remains to be identified."

sparser
"CSN6 S60 phosphorylation is known to increase the stability of CSN6 [ xref ]."

sparser
"Our mechanistic studies showed that EGF/Akt-mediated CSN6 S60 phosphorylation and subse quent stabilization of E6AP thereby causing p53 downregulation and promoting cell proliferation, cell transformation as well as tumorigenesis (Figure xref )."

rlimsp
"Our mechanistic studies showed that EGF/Akt-mediated CSN6 S60 phosphorylation and subse quent stabilization of E6AP thereby causing p53 downregulation and promoting cell proliferation, cell transformation as well as tumorigenesis (Figure 8)."

rlimsp
"Akt is able to associate with CSN6 and phosphorylate it at Ser60, which can reduce the ubiquitin-mediated protein degradation and turnover rate of CSN6, thereby increasing steady-state expression of CSN6."
COPS6 is phosphorylated on S148. 5 / 5
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rlimsp
"Mechanistic studies revealed that CSN6 is deregulated by epidermal growth factor receptor (EGFR) signaling, in which ERK2 binds directly to CSN6 Leu163/Val165 and phosphorylates CSN6 at Ser148."

sparser
"In CRC, CSN6 was deregulated by EGFR signaling, in which ERK2 bound directly to CSN6 Leu163/Val165 and phosphorylated CSN6 at Ser148."

sparser
"Mechanistic studies revealed that CSN6 is deregulated by EGFR signaling, in which ERK2 binds directly to CSN6 Leu163/Val165 and phosphorylates CSN6 at Ser148."

sparser
"The EGF-ERK2 Axis Phosphorylates CSN6 on S148 and Enhances CSN6 Stabilization."

sparser
"Epidermal-growth-factor (EGF)-induced extracellular signal-regulated kinase 2 (ERK2) activation phosphorylates CSN6 at S148, leading to β-catenin stabilization through blocking β-TrCP and colorectal cancer development [ xref ]."
COPS6 affects CARD16
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COPS6 binds CARD16.
| 5 17
| 5 17

sparser
"Recently, CSN6's interaction with COP1 is characterized in mammalian cells, and CSN6-COP1 axis is involved in 14-3-3σ degradation [ xref ]."

sparser
"These observations led us to investigate the functional relevance of the interaction of CSN6 and COP1. xref shows that CSN6 associates with COP1 endogenously as assayed by co-ip and an In vitro binding assay confirms that CSN6 directly binds to COP1."

sparser
"To address the significance of the CSN6 and COP1 interaction, we examined the impact of CSN6 on COP1 levels and noted that the steady-state level of COP1 increased when CSN6 was overexpressed ( xref )."

sparser
"Co-IP shows that CSN6 associates with COP1 endogenously and in vivo binding assay confirms that CSN6 directly binds to COP1 [ xref ]."

sparser
"The results illustrate a pathway regulation of FOXO4‐mediated serine/glycine metabolism through the function of CSN6COP1 axis."

sparser
"Given that CSN6 associates with COP1 and that COP1 co-elutes with 14-3-3σ in gel filtration assays ( xref ), we reasoned that COP1 could be involved in CSN6-mediated 14-3-3σ ubiquitination."

sparser
"For example, the stability of the p53-regulated protein 14-3-3σ, a cell cycle regulator, appears to be regulated through interactions with CSN6 and COP1 [ xref ]."

sparser
"One study demonstrated that the CSN6COP1 axis plays a critical role in controlling the stability of p27 [ xref ]."

reach
"Recently, CSN6 's interaction with COP1 is characterized in mammalian cells, and CSN6-COP1 axis is involved in 14-3-3sigma degradation [XREF_BIBR]."

reach
"These observations led us to investigate the functional relevance of the interaction of CSN6 and COP1."
COPS6 activates CARD16.
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COPS6 activates CARD16. 4 / 4
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"Mechanistic studies show that CSN6 binds and regulates FOXO4 stability through enhancing the E3 ligase activity of COP1, and that COP1 directly interacts with FOXO4 through a VP motif on FOXO4 and accelerates the ubiquitin-mediated degradation of FOXO4."

reach
"How CSN6 mediates stabilization of COP1 and if this effect is driven by the holo-CSN were not investigated in this study."

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"The mRNA levels of COP1 were not affected by CSN6 expression in a real-time quantitative PCR analysis (XREF_SUPPLEMENTARY), suggesting that CSN6 up-regulates COP1 at the post-transcriptional level."

reach
"These data imply that CSN6 possibly regulates the gene expression of SGOC genes and impacts SGOC amino acid metabolism through its negative impact on FOXO4 that transcriptionally suppresses the gene expression of SGOC genes.2.6 PKB/Akt-Mediated CSN6 Phosphorylation Enhances COP1-Regulated FOXO4 Ubiquitination and Subsequent Elevation of SGOC Genes."
COPS6 affects FASN
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COPS6 binds FASN.
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| 2 14

sparser
"Wei et al [ xref ] reported that COP9 signalosome subunit 6 (CSN6) and the tumor suppressor F-box and WD repeat domain-containing 7 (FBXW7β) prevented the degradation of FASN; as a result, the CSN6-FASN axis eventually promoted lipogenesis and tumor growth."

sparser
"Wei et al. found that CSN6 binds to both FBXW7β and FASN, and antagonizes the activity of FBXW7β by enhancing its self-ubiquitination and degradation, thereby preventing FBXW7β-mediated ubiquitination and degradation of FASN, actively regulating lipogenesis."

sparser
"Furthermore, the EGF-regulated CSN6-FASN axis is a reason for poor prognosis in CRC."

sparser
"Mechanistic studies show that CSN6 associates with both FBXW7β and FASN, and antagonizes FBXW7β’s activity by enhancing FBXW7β autoubiquitination and degradation, which in turn prevents FBXW7β-mediated FASN ubiquitination and degradation, thereby regulating lipogenesis positively."

sparser
"Both CSN6 and FASN are positively correlated in CRC, and CSN6-FASN axis, regulated by EGF, is responsible for poor prognosis of CRC."

sparser
"Thus, CSN6-FASN axis reprograms lipogenesis to promote tumor growth and is a target for cancer intervening strategy in CRC."

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"Co-immunoprecipitation (co-IP) assay revealed that CSN6 could bind to endogenous FASN in HCT 116 cells (Fig. 2b)."

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"To further support that CSN6 and FASN can form a complex in vivo, cellular proteins extracted from HCT-8 and HCT 116 cells were fractionated by gel-filtration chromatography for analysis."

sparser
"The aberrant overexpression of CSN6-FASN axis in CRC leads to the metabolic reprogramming of lipid biosynthesis that instigates tumor growth and organoid formation, and results in poor cancer prognosis."

sparser
"To further validate CSN6-FASN axis in terms of clinical significance, we analyzed the protein levels of FASN and CSN6 in CRC cell lines or tumor tissues."
COPS6 increases the amount of FASN.
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COPS6 increases the amount of FASN. 3 / 3
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"Further, CSN6 KD tumors demonstrate decreased FASN protein levels based on immunohistochemistry (IHC) staining (Fig. 6i) and immunoblotting (Supplementary Fig. 4h), low BODIPY staining (Fig. 6j) and relatively low levels of tumor cell growth (as measured by Ki67 staining) (Fig. 6i), while CSN6-reexpressing tumors can restore the levels of FASN (Fig. 6i, Supplementary Fig. 4h), increase BODIPY staining (Fig. 6j), and elevate Ki67 staining concurrently (Fig. 6i)."

reach
"We previously have shown that CSN6 is a positive regulator of c-Myc, it remains to been seen whether CSN6 can enhance FASN gene expression through c-Myc although we have observed that CSN6 have no impact on FASN mRNA expression."

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"Indeed, CSN6 overexpression increased the protein level of FASN, but not affected the mRNA expression level of FASN in several CRC cell lines (Fig. 2f)."
COPS6 inhibits FASN.
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COPS6 inhibits FASN. 2 / 2
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"Wei et al[36] reported that COP9 signalosome subunit 6 (CSN6) and the tumor suppressor F-box and WD repeat domain-containing 7 (FBXW7β) prevented the degradation of FASN; as a result, the CSN6-FASN axis eventually promoted lipogenesis and tumor growth."

reach
"We further showed that CSN6 KD accelerates FASN protein turnover (Fig. 2g), and that overexpressing CSN6 leads to reduced turnover rate of FASN (Fig. 2h)."
COPS6 decreases the amount of FASN.
| 2
COPS6 decreases the amount of FASN. 2 / 2
| 2

reach
"The significance of this interaction was demonstrated in that dox-induced CSN6 KD led to increased steady-state-expression of FBXW7β with concurrent decreased expression of FASN (Supplementary Fig. 3b), while CSN6 overexpression reduced FBXW7β and resulted in increased FASN level (Supplementary Fig. 3c)."

reach
"Consistently, CSN6 decreased the poly-ubiquitination level of FASN in a dose-dependent manner (Fig. 2i)."
COPS6 activates FASN.
| 2
COPS6 activates FASN. 2 / 2
| 2

reach
"Since both EGF and CSN6 signaling pathways can promote FASN accumulation, it is reasonable to deduce that inhibiting EGF signaling by cetuximab treatment plus attenuating FASN activity by orlistat might synergistically suppress CSN6-high CRC growth."

reach
"Together, our studies demonstrate that the role of EGF/CSN6 in increasing FASN accumulation and promoting CRC malignancy can be recapitulated in clinical samples."
AKT affects COPS6
2 | 16 3 3
AKT phosphorylates COPS6.
1 | 9 2 3
AKT phosphorylates COPS6 on S60. 8 / 8
1 | 6 1

rlimsp
"We previously showed that S60 of CSN6 has an Akt phosphorylation site [21] and that Akt increases the steady-state expression of CSN6 [21], which in turn will be translating into E6AP stabilization."

reach
"XREF_BIBR They demonstrate that Akt directly interacts with and phosphorylates CSN6 at Ser60, which inhibits the degradation of CSN6 by reducing its ubiquitination."

"Mechanistic studies show that akt causes csn6 phosphorylation at ser 60, which, in turn, reduces ubiquitin-mediated protein degradation of csn6."

reach
"XREF_BIBR Interestingly, CSN6 can be phosphorylated by Akt at Ser60, which renders CSN6 more stabilized."

reach
"S60 of CSN6 is phosphorylated by PKB/Akt 16 and is critical for CSN6 stabilization."

reach
"Mechanistic studies show that Akt causes CSN6 phosphorylation at Ser 60, which, in turn, reduces ubiquitin mediated protein degradation of CSN6."

reach
"First, we have shown that S60 of CSN6 can be phosphorylated by PKB/Akt 16 and that PKB/Akt enhances the steady‐state expression of CSN6."

reach
"19 We previously found that AKT can directly phosphorylate CSN6 at S60."
AKT phosphorylates COPS6. 7 / 7
| 3 2 2

sparser
"As for the phosphorylation event, Akt can phosphorylate CSN6 and MDM2."

rlimsp
"For instance, Akt can positively regulate CSN6 by phosphorylation."

sparser
"During EGF stimulation, CSN6 is phosphorylated by protein kinase B. This rescues CSN6 from ubiquitinylation and degradation [ 43 ] and induces a stabilization of the CRL4 substrate receptor COP1, whic[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"As for the phosphorylation event, Akt can phosphorylate CSN6 and MDM2."

reach
"To determine whether the PKB/Akt‐mediated CSN6 phosphorylation affects FOXO4 stability, we examined the steady‐state expression of FOXO4 and COP1 in the presence of CSN6S60A, a construct with no PKB/Akt phosphorylation site."

reach
"These results indicate that PKB/Akt‐mediated CSN6 phosphorylation is critical in stabilizing COP1, thereby enhancing ubiquitination level of FOXO4."

rlimsp
"As for the phosphorylation event, Akt can phosphorylate CSN6 and MDM2."
AKT activates COPS6.
1 | 4
AKT activates COPS6. 5 / 6
1 | 4

reach
"For instance, Akt can positively regulate CSN6 by phosphorylation."

"Mechanistic studies show that akt causes csn6 phosphorylation at ser 60, which, in turn, reduces ubiquitin-mediated protein degradation of csn6."

reach
"Our mechanistic studies showed that EGF and Akt mediated CSN6 S60 phosphorylation and subse quent stabilization of E6AP thereby causing p53 downregulation and promoting cell proliferation, cell transformation as well as tumorigenesis."

reach
"We previously showed that S60 of CSN6 has an Akt phosphorylation site [XREF_BIBR] and that Akt increases the steady-state expression of CSN6 [XREF_BIBR], which in turn will be translating into E6AP stabilization."

reach
"Hence, this study has further strengthened the significance of the MDM2-p53 pathway on tumorigenesis through a novel link of Akt mediated CSN6 activation."
AKT increases the amount of COPS6.
| 3
AKT increases the amount of COPS6. 3 / 3
| 3

reach
"First, we have shown that S60 of CSN6 can be phosphorylated by PKB/Akt 16 and that PKB/Akt enhances the steady‐state expression of CSN6."

reach
"Fig. 2 B shows that PDGF increased the protein level of CSN6 in a time-dependent manner, and peak time for 10 ng/ml PDGF up-regulation of CSN6 was 10 min, suggesting that CSN6 is up-regulated in PASMC[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"We previously showed that S60 of CSN6 has an Akt phosphorylation site and that Akt increases the steady-state expression of CSN6."
AKT binds COPS6.
| 1
| 1

sparser
"Akt is able to associate with CSN6 and phosphorylate it at Ser60, which can reduce the ubiquitin-mediated protein degradation and turnover rate of CSN6, thereby increasing steady-state expression of CSN6."
COPS6 affects TRIM21
| 12 11
COPS6 binds TRIM21.
| 4 10
| 4 10

sparser
"Notably, studies have unveiled intriguing interactions, for instance, PKMYT1's physical interaction with β-catenin, which stabilizes β-catenin protein and activates Wnt signaling, bolstering non-small cell lung cancer CSC self-renewal. xref Additionally, the CSN6-TRIM21 axis has surfaced, spotlighting its role in driving cancer stemness during tumorigenesis."

sparser
"Besides, the signaling axis of CSN6-TRIM21 was reported to promote tumor stemness during tumorigenesis ( xref )."

reach
"To verify Cullin 's involvement, we first showed that TRIM21 interacted with CUL1 and CSN6 by Co-IP."

sparser
"TRIM21 binding with CSN6 is enhanced by the presence of CUL1 (Fig.  xref )."

reach
"TRIM21 binding with CSN6 is enhanced by the presence of CUL1."

sparser
"In addition, TRIM21’s function is associated with autoimmune diseases, such as systemic lupus erythematosus (SLE) and Sjögren’s syndrome. xref , xref Notably, patients with SLE or Sjögren’s syndrome have an increased risk for developing certain cancers, including non-Hodgkin’s lymphoma. xref In addition, TRIM21 interacts with endoglin, xref which is a prognostic marker in CRC xref and also act as a CSC marker in renal cell carcinoma. xref Future studies will need to address how the CSN6TRIM21 axis may impact these signalling pathways to promote tumorigenesis."

sparser
"This finding aligns with a previous report that the CSN6-TRIM21 axis drives colorectal cancer stemness [ xref ]."

sparser
"In conclusion, we validate a pathway for cancer stemness regulation involving ALDH1A1 levels through the CSN6TRIM21 axis, which may be utilised as CRC molecular markers and be targeted for therapeutic intervention in cancers."

sparser
"Then we confirmed that CSN6 could interact with TRIM21 in HCT116 cells by Co-IP (Fig.  xref )."

sparser
"The MPN domain (41–174 aa) is located in N-terminal and is indispensable in CSN6’s impact on Cullin-1 neddylation. xref Then we further identified the domain of CSN6 responsible for its interaction with TRIM21 and found that the MPN domain of CSN6 bound to TRIM21 (Fig.  xref )."
COPS6 decreases the amount of TRIM21.
| 4
COPS6 decreases the amount of TRIM21. 4 / 4
| 4

reach
"In addition, knockdown of CSN6 increased TRIM21 protein levels."

reach
"We first identified that overexpression of CSN6 reduced the steady-state expression of TRIM21 in a dose dependent manner in the DLD-1 cell line."

reach
"In HCT116 cells, CSN6 reduced the steady-state level of TRIM21, and MLN4924 treatment reversed this effect."

reach
"45 Interestingly, CSN6 is able to decrease the steady-state expression of TRIM21 by enhancing TRIM21 ubiquitination, thereby promoting cancer stemness."
COPS6 inhibits TRIM21.
| 2
COPS6 inhibits TRIM21. 2 / 2
| 2

reach
"Together, these results indicate that CSN6 promoted TRIM21 degradation is dependent on TRIM21 self ubiquitination via K48 ubiquitin linkage."

reach
"We have shown that CSN6 promotes ubiquitin-dependent degradation of TRIM21, and it is then possible that CSN6 can regulate FASN stability through TRIM21 as well."
COPS6 deubiquitinates TRIM21.
| 2
COPS6 leads to the deubiquitination of TRIM21. 2 / 2
| 2

reach
"45 Interestingly, CSN6 is able to decrease the steady-state expression of TRIM21 by enhancing TRIM21 ubiquitination, thereby promoting cancer stemness."

reach
"Moreover, MLN4924 treatment abrogated TRIM21 ubiquitination promoted by CSN6."
COPS6 ubiquitinates TRIM21.
| 1
COPS6 ubiquitinates TRIM21. 1 / 1
| 1

sparser
"In summary, we performed mechanistic studies illustrating the role of CSN6-facilitated TRIM21 ubiquitination in enhancing ALDH1A1 expression via the OCT1 transcription factor, lending support to the means by which CSN6 activity can lead to the initiation of cancer stemness."
COPS6 affects MYC
| 22
COPS6 activates MYC.
| 8
COPS6 activates MYC. 8 / 9
| 8

reach
"Noticeably, reduced expression of Csn6 in Emicro-Myc background can also contribute to p53 stabilization in 33% of Emicro-Myc and Csn6 +/- lymphomas when compared with Emicro-Myc and Csn6 +/+, suggesting that both CSN6 mediated p53 downregulation and CSN6 mediated Myc elevation contribute to lymphomagenesis of this system (XREF_FIG)."

reach
"CSN6 mediated Myc stabilization translated to increased Myc transcriptional activity, as evidenced by increasing Myc luciferase reporter gene activity (XREF_FIG) and qRT-PCR for expression of Myc target genes, including CdC25A, Adm-1, and Odc-1 (XREF_FIG)."

reach
"While CSN6 expression increased Myc transcriptional activity, CSN6 knockdown by shRNA reduced Myc transcriptional activity (XREF_FIG)."

reach
"We have shown that CSN6 increases Myc stability."

reach
"Thus our study has provided a novel mechanistic answer to how CSN6 can destabilize Fbxw7 and subsequently increase the stability of Myc."

reach
"CSN6 increase Myc stability by reducing Myc ubiquitination."

reach
"Accordingly, CSN6 mediated Myc increases occurred only in HCT116 Fbxw7 +/+ cells (XREF_FIG)."

reach
"Furthermore, Fbxw7 deficiency compromised CSN6 shRNA enhanced Myc ubiquitination (XREF_FIG), suggesting that CSN6 mediated Myc stabilization is dependent on Fbxw7."
COPS6 increases the amount of MYC.
| 4
Modified COPS6 increases the amount of MYC. 2 / 2
| 2

reach
"Overexpression of CSN6 reduced the protein levels of Fbxw7alpha and increased Myc protein levels; while CSN6 knockdown by shRNA resulted in increased protein levels of Fbxw7alpha and decreased protein levels of Myc in two cell lines HCT116 and U2OS cells (XREF_FIG)."

reach
"Overexpression of CSN6 increased the mRNA levels of Myc transcriptional targets (CDC25A, cyclin D1, and Tert) and decreased the mRNA levels of Myc transcriptionally repressed genes (p27Kip1, CDKN2B, and p21Cip1), while loss of CSN6 reversed the expression of these Myc target genes."
COPS6 increases the amount of MYC. 2 / 2
| 2

reach
"Compared with wt CSN6, which clearly decreased Fbxw7 expression and increased Myc expression in U2OS cells, the MPN-KQV CSN6 mutant lost these functions (XREF_SUPPLEMENTARY)."

reach
"More importantly, overexpression of CSN6 increased c-Myc protein level in Tespa1 HSCs but not in Tespa1 HSCs, indicating that CSN6 stabilizes c-Myc in the presence of Tespa1."
COPS6 ubiquitinates MYC.
| 3
COPS6 leads to the ubiquitination of MYC. 3 / 3
| 3

reach
"In mouse experiments, COPS6 was determined to increase tumor growth by decreasing the ubiquitination of Myc and enhancing the degradation of Fbxw7."

reach
"Because Myc is degraded through the ubiquitin pathway 38, we examined whether ubiquitination is involved and found that CSN6 overexpression inhibited Myc ubiquitination while shRNA-CSN6 increased Myc ubiquitination (XREF_FIG)."

reach
"Furthermore, Fbxw7 deficiency compromised CSN6 shRNA enhanced Myc ubiquitination (XREF_FIG), suggesting that CSN6 mediated Myc stabilization is dependent on Fbxw7."
COPS6 inhibits MYC.
| 3
COPS6 inhibits MYC. 3 / 3
| 3

reach
"Compared with empty vector transfected control cells, cells transfected with CSN6 had a decelerated endogenous Myc turnover rate (XREF_FIG); the pulse chase analyses of over expressed Myc with CSN6 or with shRNA mediated CSN6 knockdown indicated that over expression of CSN6 stabilized Myc while shRNA-CSN6 increased Myc degradation (XREF_FIG)."

reach
"Knockdown of CSN6 decreased the steady-state expression of Myc protein in HCT116 Fbxw7 +/+ but not in HCT116 Fbxw7 -/- cells (XREF_FIG)."

reach
"While CSN6 expression increased Myc transcriptional activity, CSN6 knockdown by shRNA reduced Myc transcriptional activity (XREF_FIG)."
COPS6 decreases the amount of MYC.
| 2
Modified COPS6 decreases the amount of MYC. 2 / 2
| 2

reach
"Overexpression of CSN6 reduced the protein levels of Fbxw7alpha and increased Myc protein levels; while CSN6 knockdown by shRNA resulted in increased protein levels of Fbxw7alpha and decreased protein levels of Myc in two cell lines HCT116 and U2OS cells (XREF_FIG)."

reach
"Overexpression of CSN6 increased the mRNA levels of Myc transcriptional targets (CDC25A, cyclin D1, and Tert) and decreased the mRNA levels of Myc transcriptionally repressed genes (p27Kip1, CDKN2B, and p21Cip1), while loss of CSN6 reversed the expression of these Myc target genes."
COPS6 binds MYC.
| 2
| 2

reach
"These data show that the association between CSN6 and Myc is present in many types of human cancer, suggesting that the positive regulation of Myc by CSN6 may be a clinically relevant regulatory pathway in human cancers (XREF_FIG)."

reach
"First, we found that CSN6 associated with Myc, Fbxw7 and Cullin-1 in U2OS cells in vivo (XREF_FIG), suggesting that CSN6 interacts with SCF complex and may regulate it to stabilize Myc."
COPS6 affects CTNNB1
| 21
COPS6 increases the amount of CTNNB1.
| 7
COPS6 increases the amount of CTNNB1. 5 / 6
| 5

reach
"CSN6 increased the steady-state expression of beta-catenin in a dose dependent manner while downregulating beta-Trcp (XREF_FIG)."

reach
"CSN6 positively regulated beta-catenin expression in a beta-Trcp-dependent manner and triggered expression of several EMT related genes regulated by beta-catenin."

reach
"Together, the results suggest that CSN6 may positively regulate beta-catenin transcription, thereby mediating the EMT."

reach
"CSN6 positively regulates beta-catenin expression in a beta-Trcp-dependent manner and triggers the expression of several EMT related genes regulated by beta-catenin."

reach
"CSN6 positively regulates beta-catenin expression."
Modified COPS6 increases the amount of CTNNB1. 2 / 2
| 2

reach
"Overexpression of CSN6 could increase the expression of beta-catenin target genes (XREF_SUPPLEMENTARY)."

reach
"Furthermore, continuous knockdown of CSN6 expression in K1 and PTC cells decreased the beta-catenin protein levels, whereas the restoration of beta-catenin expression using an expression vector (Cat-pcDNA, Cat) increased expression of both vimentin mRNA and protein to some extent."
COPS6 activates CTNNB1.
| 8
COPS6 activates CTNNB1. 8 / 8
| 8

reach
"CSN6 positively regulates beta-catenin protein stability and facilities the EMT in PTC cells."

reach
"CSN6 Positively Regulates beta-Catenin Protein Stability and Facilitates the Transcriptional Activity of beta-Catenin."

reach
"Here, we provided another example of CSN6 preserved Cullin neddylation in affecting another F-box protein -- beta-Trcp and showed how CSN6 can destabilize beta-Trcp and subsequently increase the stability of beta-catenin."

reach
"The TOP-FLASH TCF/LEF -1 luciferase reporter gene analyses showed that Flag-CSN6 significantly increased beta-catenin transactivation but the activity of Flag-CSN6-S148A was compromised (XREF_FIG)."

reach
"Given that CSN6 positively regulates beta-catenin by regulating beta-Trcp ubiquitination, 41 CSN6 may also regulate ALDH1A1 expression through the beta-catenin pathway."

reach
"Because KRAS is an upstream regulator of these two pathways, KRAS mutations will continue to activate both RAS-MAPK and AKT pathways to stabilize CSN6, which in turn activates beta-catenin activity to cause pathogenesis in colon cancer."

reach
"Downregulation of CSN6 attenuates papillary thyroid carcinoma progression by reducing Wnt and beta-catenin signaling and sensitizes cancer cells to FH535 therapy."

reach
"Furthermore, we demonstrate that CSN6 enhances beta-catenin stability and that the CSN6 associated protein beta-Trcp negatively regulates beta-catenin stability."
COPS6 inhibits CTNNB1.
| 4
COPS6 inhibits CTNNB1. 4 / 4
| 4

reach
"The CSN6 knockdown mediated beta-catenin destabilization translated into beta-catenin 's reduced transcriptional activity, as evidenced by qRT-PCR findings showing diminished expression of beta-catenin target genes, including myc, cyclin D, and vascular endothelial growth factor (XREF_FIG)."

reach
"As expected, we found that the beta-catenin level decreased and that of beta-Trcp increased when cells were treated with CSN6-shRNA (CSN6 knockdown) (Fig XREF_FIG A and B)."

reach
"CSN6 overexpression reduced the turnover rate of beta-catenin and increased the turnover rate of beta-Trcp (XREF_FIG)."

reach
"Furthermore, we demonstrate that CSN6 enhances beta-catenin stability and that the CSN6 associated protein beta-Trcp negatively regulates beta-catenin stability."
COPS6 decreases the amount of CTNNB1.
| 2
COPS6 decreases the amount of CTNNB1. 2 / 2
| 2

reach
"Congruently, CSN6 knockdown xenograft tumors had elevated beta-Trcp and diminished beta-catenin expression and a slower growth rate."

reach
"Furthermore, CSN6 increased the ubiquitination level of beta-Trcp and reduced the ubiquitination level of beta-catenin (XREF_FIG)."
CARD16 affects COPS6
| 5 17
| 5 17

sparser
"Recently, CSN6's interaction with COP1 is characterized in mammalian cells, and CSN6-COP1 axis is involved in 14-3-3σ degradation [ xref ]."

sparser
"These observations led us to investigate the functional relevance of the interaction of CSN6 and COP1. xref shows that CSN6 associates with COP1 endogenously as assayed by co-ip and an In vitro binding assay confirms that CSN6 directly binds to COP1."

sparser
"To address the significance of the CSN6 and COP1 interaction, we examined the impact of CSN6 on COP1 levels and noted that the steady-state level of COP1 increased when CSN6 was overexpressed ( xref )."

sparser
"Co-IP shows that CSN6 associates with COP1 endogenously and in vivo binding assay confirms that CSN6 directly binds to COP1 [ xref ]."

sparser
"The results illustrate a pathway regulation of FOXO4‐mediated serine/glycine metabolism through the function of CSN6COP1 axis."

sparser
"Given that CSN6 associates with COP1 and that COP1 co-elutes with 14-3-3σ in gel filtration assays ( xref ), we reasoned that COP1 could be involved in CSN6-mediated 14-3-3σ ubiquitination."

sparser
"For example, the stability of the p53-regulated protein 14-3-3σ, a cell cycle regulator, appears to be regulated through interactions with CSN6 and COP1 [ xref ]."

sparser
"One study demonstrated that the CSN6COP1 axis plays a critical role in controlling the stability of p27 [ xref ]."

reach
"Recently, CSN6 's interaction with COP1 is characterized in mammalian cells, and CSN6-COP1 axis is involved in 14-3-3sigma degradation [XREF_BIBR]."

reach
"These observations led us to investigate the functional relevance of the interaction of CSN6 and COP1."
| 3 18
| 3 17

reach
"As shown in Fig. 3 , overexpression of FOXA1 could enhance CFPAC-1 cells’ invasion and metastasis abilities, and the re-expression of FOXA1 could attenuate the decrease of invasion and metastasis indu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Our results showed that knocking down FOXA1 inhibited the increase of invasion and metastasis abilities stimulated by overexpression of CSN6 ( Fig. 3 g-j)."

reach
"These results suggested that CSN6-mediated high expression of FOXA1 had an important role in metastasis of PAAD.Next, we sought to figure out the molecular mechanism of which CSN6 regulate the express[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Furthermore, we identified that CSN6 enhancement leads to Snail1 stabilization and promotes metastasis of breast cancer cells through inhibiting Snail1 ubiquitination."

reach
"CSN6 promotes tumor metastasis via up-regulating snail1 in xenograft model."

reach
"Our study reveals that CSN6 promotes the migration and metastasis of breast cancer cells, indicating that CSN6 functions as an oncogene in breast cancer cells."

reach
"Importantly, inhibition of CSN6 decreased the metastasis of PAAD to liver."

eidos
"CSN6 promotes tumor metastasis via up-regulating snail1 in xenograft model To identify the role of CSN6 in the metastatic potency of breast cancer cells in vivo , lentiviruses packed with CSN6-NC or CSN6 overexpression vectors were transfected into MDA-MB-231 cell lines , and the fluorescence intensity was detected by Olympus light microscope ( Figure 4A ) ."

eidos
"The results demonstrated that CSN6 promoted tumor metastasis in vivo and up-regulated the protein level of Snail1 , which was consistent with the results mentioned in vitro experiments ."

reach
"Our data demonstrated that CSN6 could promote metastasis of CC cells via CTSL."
| 1

reach
"Meanwhile, the down-regulation of CSN6 inhibited the metastasis of cancer cells."
COPS6 affects FBXW7
| 19 2
COPS6 ubiquitinates FBXW7.
| 5 1
COPS6 leads to the ubiquitination of FBXW7. 6 / 6
| 5 1

reach
"On the other hand, CSN6 increased the ubiquitination of FBXW7β (Fig. 3n); therefore, FBXW7β-mediated FASN ubiquitination level can be hindered by CSN6 overexpression (Fig. 3o)."

reach
"Further, CSN6 KD reduced the turnover rate of FBXW7β (Supplementary Fig. 3f), and overexpressing CSN6 increased the ubiquitination of FBXW7β (Supplementary Fig. 3g)."

reach
"These data suggest that CSN6 increases FBXW7β ubiquitination through enhancing autoubiquitination of FBXW7β, thereby reducing the steady-state expression of FBXW7β."

reach
"In vitro ubiquitination assays also showed that CSN6 enhanced Fbxw7 ubiquitination and diminished Fbxw7 mediated ubiquitinations of Fbxw7 's targets, Myc and cyclin E (XREF_SUPPLEMENTARY)."

reach
"COP9 signalosome complex subunit 6 promotes FBXW7 autoubiquitination and proteasome-mediated degradation."

sparser
"In vitro ubiquitination assays also showed that CSN6 enhanced Fbxw7 ubiquitination and diminished Fbxw7-mediated ubiquitinations of Fbxw7’s targets, Myc and cyclin E ( xref )."
COPS6 inhibits FBXW7.
| 5
COPS6 inhibits FBXW7. 5 / 5
| 5

reach
"Finally, to link CSN6 to the event, we showed that GSK3β (S9A)-enhanced FASN ubiquitination is attenuated by the expression of CSN6 (Fig. 4m), suggesting that CSN6 antagonized FBXW7β/GSK3β-mediated FASN ubiquitination.To determine the phenotypic effect of GSK3β in governing FBXW7β-mediated FASN degradation."

reach
"The significance of this interaction was demonstrated in that dox-induced CSN6 KD led to increased steady-state-expression of FBXW7β with concurrent decreased expression of FASN (Supplementary Fig. 3b), while CSN6 overexpression reduced FBXW7β and resulted in increased FASN level (Supplementary Fig. 3c)."

reach
"Further, CSN6 KD reduced the turnover rate of FBXW7β (Supplementary Fig. 3f), and overexpressing CSN6 increased the ubiquitination of FBXW7β (Supplementary Fig. 3g)."

reach
"In mouse experiments, Chen et al[20] demonstrated that COPS6 enhances Fbxw7 degradation through binding, thereby maintaining Myc stability and promoting tumor progression."

reach
"Notably, CSN6 negatively regulates FBXW7β through enhancing FBXW7β autoubiquitination, thereby stabilizing FASN."
COPS6 decreases the amount of FBXW7.
| 3
COPS6 decreases the amount of FBXW7. 3 / 3
| 3

reach
"It is important to point out that CSN6 interacted with all the three isoforms of Fbxw7 XREF_BIBR, XREF_BIBR and reduced the steady-state expression of Fbxw7 in a dose dependent manner (XREF_SUPPLEMENTARY)."

reach
"These data suggest that CSN6 decreases FBXW7β protein level through ubiquitination regulation in order to prevent FBXW7β-mediated FASN degradation.FBXW7, including FBXW7β, is rapidly degraded by an autoubiquitination mechanism."

reach
"Compared with wt CSN6, which clearly decreased Fbxw7 expression and increased Myc expression in U2OS cells, the MPN-KQV CSN6 mutant lost these functions (XREF_SUPPLEMENTARY)."
COPS6 binds FBXW7.
| 2 1
| 2 1

reach
"The binding of COP9 signalosome subunit 6 (CSN6) to FBXW7β inhibits the degradation of fatty acid synthase (FASN), promoting lipogenic CRC and tumor progression (Wei et al., 2023)."

sparser
"CSN6’s association with Fbxw7 facilitates its degradation."

reach
"First, we found that CSN6 associated with Myc, Fbxw7 and Cullin-1 in U2OS cells in vivo (XREF_FIG), suggesting that CSN6 interacts with SCF complex and may regulate it to stabilize Myc."
COPS6 deubiquitinates FBXW7.
| 2
COPS6 leads to the deubiquitination of FBXW7. 2 / 2
| 2

reach
"Previous study has shown that CSN6 enhanced the autoubiquitination and degradation of FBXW7 (a subunit of SCF ubiquitin ligase) 35, which could target mTOR and regulate its ubiquitination and degradation processes 36."

reach
"CSN6 promotes c-Myc-driven tumorigenesis by enhancing the neddylation of Cullin-1 and facilitating autoubiquitination/degradation of Fbxw7, which stabilizes c-Myc and upregulates Myc target genes [72][MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
COPS6 activates FBXW7.
| 2
COPS6 activates FBXW7. 2 / 2
| 2

reach
"Consistently, CSN6 knockdown decreased turnover rate of Fbxw7 (XREF_FIG and XREF_SUPPLEMENTARY)."

reach
"We next addressed whether Cullin-1 is involved in CSN6 mediated Fbxw7 downregulation."
COPS6 affects CUL1
8 1 | 11 1
COPS6 binds CUL1.
8 1 | 2 1
8 1 | 2 1

No evidence text available

sparser
"These findings highlight the complexity of TRIM21 regulation and indicate that CSN6-CUL1 regulation is involved in TRIM21 ubiquitination."

No evidence text available

No evidence text available

reach
"First, we found that CSN6 associated with Myc, Fbxw7 and Cullin-1 in U2OS cells in vivo (XREF_FIG), suggesting that CSN6 interacts with SCF complex and may regulate it to stabilize Myc."

No evidence text available

No evidence text available

reach
"We found that both CSN6 and CSN5 associated with Cullin-1 and that CSN6 and CSN5 competed with each other for Cullin-1 binding in a dose dependent manner (XREF_FIG)."

No evidence text available

No evidence text available
COPS6 activates CUL1.
| 9
COPS6 activates CUL1. 9 / 9
| 9

reach
"35 Interestingly, CSN6 can increase CUL1 neddylation."

reach
"CSN6 knockdown reduced the neddylated form of Cullin-1, leading to accumulation of Fbxw7, which in turn caused downregulation of Myc (XREF_FIG)."

reach
"We further showed that overexpression of dominant negative Ubc12 (dnUbc12-HA) XREF_BIBR, XREF_BIBR, which antagonized CSN6 mediated increase of Cullin-1 neddylation, compromised CSN6 mediated Fbxw7alpha downregulation (XREF_FIG)."

reach
"CSN6 enhanced neddylation of Cullin-1 and facilitated auto-ubiquitination and degradation of Fbxw7, a component of CRL involved in Myc ubiquitination, thereby stabilizing Myc."

reach
"Together, these data suggest that CSN6 increases Cullin-1 neddylation by competing with CSN5 for binding to Cullin-1 through the MPN domain, thereby facilitating Cullin-1-enhanced Fbxw7 degradation."

reach
"We then found that increasing amounts of CSN6 enhanced neddylation of wild type Cullin-1 but not the Cullin-1 mutant 41 in which the Nedd-8-conjugating site lysine (K) 720 was mutated to arginine (R) (XREF_SUPPLEMENTARY)."

reach
"On the other hand, mono-allelic deletion of CSN6 decreases Cullin1 neddylation, stabilizes FBXW7, and compromises lymphomagenesis in an Emu-Myc mouse model [XREF_BIBR]."

reach
"The MPN domain plays an indispensable role in CSN6-mediated Cullin-1 neddylation, thereby involving in ubiquitination regulation."

reach
"Another investigation revealed that CSN6 increased neddylation of Cullin-1 to contribute to FBXW7 autoubiquitination as a K48 linkage (126)."
MAPK1 affects COPS6
1 2 | 9 5
MAPK1 binds COPS6.
2 | 4 2
2 | 2

sparser
"Mechanistic studies have shown that CSN6 is deregulated by EGFR signaling, in which ERK2 interacts with CSN6."

sparser
"In CRC, CSN6 was deregulated by EGFR signaling, in which ERK2 bound directly to CSN6 Leu163/Val165 and phosphorylated CSN6 at Ser148."

No evidence text available

No evidence text available
MAPK1 binds COPS6 and Leu163. 2 / 2
| 2

reach
"Mechanistic studies suggested that ERK2 directly binds to CSN6 Leu163/Val165 and phosphorylates COPS6 at Ser148, thereby regulating β-Trcp and stabilizing β-catenin expression, consequently blocking the ubiquitin-proteasome pathway and promoting CRC development.Programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) checkpoint blockade is an emerging immunotherapy modality in various tumors, while its regulatory mechanism remains uncertain."

reach
"Mechanistic studies revealed that CSN6 is deregulated by EGFR signaling, in which ERK2 binds directly to CSN6 Leu163 and Val165 and phosphorylates CSN6 at Ser148."
MAPK1 binds COPS6 and Ser148. 2 / 2
| 2

reach
"Mechanistic studies suggested that ERK2 directly binds to CSN6 Leu163/Val165 and phosphorylates COPS6 at Ser148, thereby regulating β-Trcp and stabilizing β-catenin expression, consequently blocking the ubiquitin-proteasome pathway and promoting CRC development.Programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) checkpoint blockade is an emerging immunotherapy modality in various tumors, while its regulatory mechanism remains uncertain."

reach
"Mechanistic studies revealed that CSN6 is deregulated by EGFR signaling, in which ERK2 binds directly to CSN6 Leu163 and Val165 and phosphorylates CSN6 at Ser148."
MAPK1 activates COPS6.
| 2 3
MAPK1 activates COPS6. 5 / 6
| 2 3

sparser
"Thus, the deregulation of β-catenin by ERK2-activated CSN6 is crucial for CRC development xref ."

sparser
"Fang et al. reported that ERK2-activated CSN6 regulates β-Trcp and stabilized β-catenin expression by blocking the ubiquitin-proteasome pathway, thereby promoting CRC development xref ."

sparser
"Taken together, our study’s findings indicate that the deregulation of β-catenin by ERK2-activated CSN6 is important for CRC development."

reach
"Taken together, our study 's findings indicate that the deregulation of beta-catenin by ERK2 activated CSN6 is important for CRC development."

reach
"Thus, the deregulation of β-catenin by ERK2-activated CSN6 is crucial for CRC development ."
MAPK1 phosphorylates COPS6.
1 | 3
MAPK1 phosphorylates COPS6 on S148. 4 / 5
1 | 3

reach
"Mechanistic studies suggested that ERK2 directly binds to CSN6 Leu163/Val165 and phosphorylates COPS6 at Ser148, thereby regulating β-Trcp and stabilizing β-catenin expression, consequently blocking the ubiquitin-proteasome pathway and promoting CRC development.Programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) checkpoint blockade is an emerging immunotherapy modality in various tumors, while its regulatory mechanism remains uncertain."

reach
"Mechanistic studies revealed that CSN6 is deregulated by EGFR signaling, in which ERK2 binds directly to CSN6 Leu163 and Val165 and phosphorylates CSN6 at Ser148."

No evidence text available

reach
"Epidermal-growth-factor (EGF)-induced extracellular signal-regulated kinase 2 (ERK2) activation phosphorylates CSN6 at S148, leading to β-catenin stabilization through blocking β-TrCP and colorectal cancer development [123]."
CUL4A affects COPS6
18 | 1
18 | 1

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trips
"CUL4A regulates endometrial cancer cell proliferation, invasion and migration by interacting with CSN6."

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GPS1 affects COPS6
12 2 | 1 3
12 2 | 1 3

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sparser
"It has been shown that the complex is composed of two symmetrical modules, CSN1/2/3/8 and CSN4/5/6/7, connected by interactions between CSN1 and CSN6 [ xref ]."

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sparser
"In this study Sharon et al. found that the structure of the complex is arranged by two symmetrical clusters/modules, CSN1/2/3/8 and CSN4/5/6/7, connected by a single link via CSN1CSN6."

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sparser
"The COP9 signalosome (CSN) is a multiprotein complex composed of eight subunits (CSN1-CSN6, CSN7A or CSN7B and CSN8)."

reach
"Additionally, correlation and enrichment analyses identified two genes, GPS1 and TCEB2, associated with COPS6, which could be further explored to understand its mechanisms."

No evidence text available

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COPS6 affects CTSL
| 16 2
COPS6 increases the amount of CTSL.
| 7
COPS6 increases the amount of CTSL. 7 / 7
| 7

reach
"We further demonstrated that CSN6 positively regulated CTSL expression through an autophagy-lysosomal system."

reach
"In addition, CSN6 could up-regulate the expression of CTSL by inhibiting autophagy, thereby promoting the migration and invasion of CC cells."

reach
"CSN6 interacts with CTSL and positively regulates CTSL expression through an autophagy-lysosomal system."

reach
"These results suggested that CSN6 increased steady-state expression of CTSL in an autophagy-lysosomal dependent manner."

reach
"Furthermore, knockdown of CSN6 suppressed CTSL expression and the cell invasion ability of the CC cells."

reach
"CSN6 could increase steady-state expression of CTSL through preventing autophagy-lysosomal system."

reach
"For instance, Zhang et al[24] revealed that the COPS6-UBR5-CDK9 axis could control melanoma proliferation and metastasis, while Mao et al[25] found that COPS6 could promote migration and invasion of cervical cancer cells by regulating the expression level of cathepsin L through the autophagy-lysosomal system."
COPS6 inhibits CTSL.
| 5
COPS6 inhibits CTSL. 5 / 5
| 5

reach
"CSN6-mediated CTSL up-regulation was decreased by Rapamycin."

reach
"In summary, our study showed that down- regulation of CSN6 inhibits invasion via autophagic degradation of CTSL in CC."

reach
"CSN6 could suppress the degradation of CTSL, then facilitated the migration and invasion of CC cells."

reach
"In contrast, overexpression of CSN6 decreased the turnover rate of CTSL (Fig. 8D)."

reach
"Taken together, we concluded that CSN6 might promote the migration and invasion of cervical cancer cells by inhibiting autophagic degradation of CTSL and serve as a potential gene therapy target for the treatment of CC metastasis."
COPS6 binds CTSL.
| 1 2
| 1 2

reach
"Co-immunoprecipitation experiments showed endogenous interaction of CSN6 and CTSL in HeLa cells (Fig. 8A and B)."

sparser
"CSN6 interacts with CTSL and positively regulates CTSL expression through an autophagy-lysosomal system."

sparser
"Co-immunoprecipitation experiments showed endogenous interaction of CSN6 and CTSL in HeLa cells (Fig. xref A and B)."
COPS6 activates CTSL.
| 3
COPS6 activates CTSL. 3 / 3
| 3

reach
"On the basis of our mechanistic studies, we developed a model of CSN6 modulating CTSL stability and metastasis (Fig. 10)."

reach
"Moreover, CSN6-mediated CTSL up-regulation was enhanced by Chloroquine."

reach
"In addition, CSN6-mediated CTSL up-regulation was enhanced by CQ (Fig. 8F)."
COPS6 affects COPS3
16 2 |
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COPS6 affects COPS2
16 2 |
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COPS3 affects COPS6
16 2 |
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COPS2 affects COPS6
16 2 |
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COPS8 affects COPS6
14 2 |
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COPS7A affects COPS6
17 |
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COPS6 affects COPS8
14 2 |
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COPS6 affects COPS7A
17 |
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COPS6 affects CDK9
| 1 11 5
COPS6 binds CDK9.
| 4 5
| 4 5

reach
"This study demonstrates that CSN6 interacts with CDK9 and increases the stability of CDK9 by controlling the expression of the E3 ligase UBR5."

reach
"CSN6 interacts with CDK9 and regulates CDK9 stability."

reach
"In summary, these results indicate that CSN6 interacts with CDK9 and regulates CDK9 stability by reducing CDK9 ubiquitination and degradation."

sparser
"This study demonstrates that CSN6 interacts with CDK9 and increases the stability of CDK9 by controlling the expression of the E3 ligase UBR5."

sparser
"CSN6 interacts with CDK9 and regulates CDK9 stability."

sparser
"A co-IP assay further validated that CSN6 interacted with CDK9 in A375 and MV3 melanoma cells (Fig. xref )."

reach
"Furthermore, we revealed that CSN6 interacted with CDK9 by co-IP."

sparser
"In summary, these results indicate that CSN6 interacts with CDK9 and regulates CDK9 stability by reducing CDK9 ubiquitination and degradation."

sparser
"Furthermore, we revealed that CSN6 interacted with CDK9 by co-IP."
COPS6 activates CDK9.
| 1 2
COPS6 activates CDK9. 3 / 3
| 1 2

reach
"We also found that CSN6 overexpression in melanoma cells could prolong the half-life of CDK9 and that the decrease in CDK9 protein expression in CSN6-knockdown cells could be obviously rescued in the presence of MG132, indicating that CSN6 regulates CDK9 stability by reducing CDK9 ubiquitination."

reach
"Furthermore, in CSN6-knockdown melanoma cells, UBR5 knockdown abrogated the effects caused by CSN6 silencing, suggesting that CSN6 activates the UBR5 and CDK9 pathway to promote melanoma cell proliferation and metastasis."

eidos
"Next , western blot analysis demonstrated that CSN6 increased CDK9 expression and decreased UBR5 expression in a dose-dependent manner in 293FT cells ( Fig. 5B ) ."
COPS6 increases the amount of CDK9.
| 2
COPS6 increases the amount of CDK9. 2 / 2
| 2

reach
"Next, western blot analysis demonstrated that CSN6 increased CDK9 expression and decreased UBR5 expression in a dose dependent manner in 293FT cells."

reach
"Our study revealed that CSN6 positively regulated the CDK9 protein level, while the CDK9 mRNA level remained unchanged, indicating that CSN6 may regulate CDK9 expression through posttranscriptional regulation."
COPS6 deubiquitinates CDK9.
| 2
COPS6 leads to the deubiquitination of CDK9. 2 / 2
| 2

reach
"CSN6 promotes melanoma proliferation and metastasis by controlling the UBR5 mediated ubiquitination and degradation of CDK9."

reach
"In addition, to investigate whether CSN6 controls the ubiquitination and degradation of the E3 ligase UBR5 to stabilize CDK9, in vivo ubiquitination assays were performed and found that CSN6 increased UBR5 ubiquitination levels and decreased CDK9 ubiquitination levels in melanoma cells and 293FT cells."
COPS6 inhibits CDK9.
| 1
COPS6 inhibits CDK9. 1 / 1
| 1

reach
"Then, we explored how CSN6 affects the stability of CDK9 and found that overexpression of CSN6 decreased the turnover rate of CDK9 in melanoma cells by using the de novo protein synthesis inhibitor cycloheximide (CHX, Sigma, USA)."
FASN affects COPS6
| 2 14
| 2 14

sparser
"Wei et al [ xref ] reported that COP9 signalosome subunit 6 (CSN6) and the tumor suppressor F-box and WD repeat domain-containing 7 (FBXW7β) prevented the degradation of FASN; as a result, the CSN6-FASN axis eventually promoted lipogenesis and tumor growth."

sparser
"Wei et al. found that CSN6 binds to both FBXW7β and FASN, and antagonizes the activity of FBXW7β by enhancing its self-ubiquitination and degradation, thereby preventing FBXW7β-mediated ubiquitination and degradation of FASN, actively regulating lipogenesis."

sparser
"Furthermore, the EGF-regulated CSN6-FASN axis is a reason for poor prognosis in CRC."

sparser
"Mechanistic studies show that CSN6 associates with both FBXW7β and FASN, and antagonizes FBXW7β’s activity by enhancing FBXW7β autoubiquitination and degradation, which in turn prevents FBXW7β-mediated FASN ubiquitination and degradation, thereby regulating lipogenesis positively."

sparser
"Both CSN6 and FASN are positively correlated in CRC, and CSN6-FASN axis, regulated by EGF, is responsible for poor prognosis of CRC."

sparser
"Thus, CSN6-FASN axis reprograms lipogenesis to promote tumor growth and is a target for cancer intervening strategy in CRC."

reach
"Co-immunoprecipitation (co-IP) assay revealed that CSN6 could bind to endogenous FASN in HCT 116 cells (Fig. 2b)."

reach
"To further support that CSN6 and FASN can form a complex in vivo, cellular proteins extracted from HCT-8 and HCT 116 cells were fractionated by gel-filtration chromatography for analysis."

sparser
"The aberrant overexpression of CSN6-FASN axis in CRC leads to the metabolic reprogramming of lipid biosynthesis that instigates tumor growth and organoid formation, and results in poor cancer prognosis."

sparser
"To further validate CSN6-FASN axis in terms of clinical significance, we analyzed the protein levels of FASN and CSN6 in CRC cell lines or tumor tissues."
COPS6 affects SIRT2
| 2 11 3
COPS6 decreases the amount of SIRT2.
| 7
COPS6 decreases the amount of SIRT2. 7 / 7
| 7

reach
"In the present study, we found that overexpression of CSN6 inhibited the mRNA and protein expression of SIRT2, and SIRT2 mediated the pro-hypertrophic function of CSN6 in cardiomyocytes response to An[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"In addition, CSN6 could inhibit the mRNA expression of SIRT2, this highlighted a question how dysregulation of deubiquitinase caused a transcriptional suppression."

reach
"Mechanistically, increased CSN6 stabilized transcription factor Nkx2.2 and then inhibiting the expression of SIRT2."

reach
"Moreover, we found that CSN6 inhibited SIRT2 expression by stabilizing the expression of Nkx2.2, a transcription factor which suppressed SIRT2 transcription [ 24 ]."

reach
"On the contrary, overexpression of CSN6 reduced the expression of SIRT2 and leaded to a decrease of AMPK activation ( Fig.S2 C)."

reach
"Interestingly, silencing CSN6 increased the mRNA and protein expression of SIRT2, and overexpression of CSN6 decreased the mRNA and protein expression of SIRT2( Fig. 3 B–E)."

reach
"Besides, silencing CSN6 not only increased the expression of SIRT2, but also leaded to the activation of AMPK ( Fig.S2 B)."
COPS6 binds SIRT2.
| 1 3
| 1 3

sparser
"Thus, we initially detected whether there was an interaction between CSN6 and SIRT2."

sparser
"Co-immunoprecipitation (co-IP) result suggested that there was no interaction between CSN6 and SIRT2 under the treatment of Ang II ( Fig.S3 A )."

reach
"Thus, we initially detected whether there was an interaction between CSN6 and SIRT2."

sparser
"But we found that there was no interaction between CSN6 and SIRT2."
COPS6 inhibits SIRT2.
| 2 1
COPS6 inhibits SIRT2. 3 / 3
| 2 1

reach
"In addition, the luciferase reporter gene assay showed that CSN6 inhibited the transcription activity of SIRT2 promoter ( Fig.S3 E)."

eidos
"CSN6 inhibited the expression of SIRT2 , and re-expression of SIRT2 attenuated the myocardial hypertrophy caused by CSN6 overexpression ."

eidos
"Further investigation discovered that CSN6 suppressed the expression of SIRT2 via up-regulating Nkx2.2 , a transcription suppressor of SIRT2 ."
COPS6 increases the amount of SIRT2.
| 2
COPS6 increases the amount of SIRT2. 2 / 2
| 2

reach
"However, the molecular mechanism of SIRT2 were down-regulated in Ang II-induced hypertrophic cardiomyocytes is still unclear.In this study, we found that CSN6 was up-regulated in hypertrophic hearts a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"A recent study reported that CSN6 is elevated in angiotensin II-induced hypertrophic mouse hearts and that the overexpression of CSN6 in neonatal rat cardiomyocytes induces hypertrophy, possibly by controlling the neddylation of CRLs and consequent regulation of SIRT2 protein levels [54]."
COPS6 affects CDKN1B
1 | 1 8 3
COPS6 binds CDKN1B.
1 | 1 2 3
1 | 1 2 3

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sparser
"Mechanistic studies showed that CSN6 interacts with p27 and enhances ubiquitin-mediated degradation of p27."

trips
"Mechanistic studies show that CSN6 interacts with p27(Kip1) and facilitates ubiquitin-mediated degradation of p27(Kip1)."

reach
"Mechanistic studies show that CSN6 interacts with p27 (Kip1) and facilitates ubiquitin mediated degradation of p27 (Kip1)."

sparser
"Mechanistic studies show that CSN6 interacts with p27(Kip1) and facilitates ubiquitin-mediated degradation of p27(Kip1)."

reach
"Mechanistic studies show that CSN6 interacts with p27 and facilitates ubiquitin mediated degradation of p27."

sparser
"Mechanistic studies show that CSN6 interacts with p27 and facilitates ubiquitin-mediated degradation of p27."
COPS6 decreases the amount of CDKN1B.
| 3
COPS6 decreases the amount of CDKN1B. 1 / 3
| 1

reach
"As expected, we found that knockdown of CSN6 markedly enhanced intracellular p27 levels starting at day 2 post-infection ( Fig. 4 A)."
Modified COPS6 decreases the amount of CDKN1B. 2 / 2
| 2

reach
"Ectopic expression of CSN6 can decrease the expression of p27 (Kip1), while CSN6 knockdown leads to p27 (Kip1) stabilization."

reach
"Ectopic expression of CSN6 decreases the expression of p27 while CSN6 knockdown leads to p27 stabilization."
COPS6 inhibits CDKN1B.
| 3
COPS6 inhibits CDKN1B. 3 / 3
| 3

reach
"CSN6 mediated p27 degradation depends on the nuclear export of p27 (Kip1), which is regulated through COP1 nuclear exporting signal."

reach
"CSN6 mediated p27 degradation depends on the nuclear export of p27, which is regulated through COP1 's nuclear exporting signal."

reach
"Ectopic expression of CSN6 can decrease the expression of p27 (Kip1), while CSN6 knockdown leads to p27 (Kip1) stabilization."
MIF affects COPS6
1 | 4 10
1 | 4 10

sparser
"For comparison of MIFCSN6 with MIF–JAB1 binding, CoIPs of endogenous MIF–JAB1 complexes were performed from HEK293mycJAB1 cells by a similar protocol, except that the mouse monoclonal anti-JAB1 antib[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Comparison with a CoIP of endogenous MIF–JAB1 complexes revealed that formation of the MIFCSN6 complexes was slightly weaker than that of the MIF–JAB1 complexes."

sparser
"Since CSN6 does not contain a functional JAMM motif within its MPN domain ( Fig. 4 A), endogenous formation of MIFCSN6 complexes confirmed that the JAMM motif and JAMM-mediated isopeptidase activity [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Since the MIFCSN6 interaction is somewhat weaker than that between MIF and JAB1/CSN5, it may be implied that JAB1 is the major interaction partner of MIF in the cell, but that additional weaker inter[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The potential interaction between MIF and CSN6 was examined by CoIP between endogenous MIF and endogenous CSN6 using an anti-CSN6 antibody for CoIP."

reach
"Comparison with a CoIP of endogenous MIF–JAB1 complexes revealed that formation of the MIFCSN6 complexes was slightly weaker than that of the MIF–JAB1 complexes."

reach
"Since CSN6 does not contain a functional JAMM motif within its MPN domain ( Fig. 4 A), endogenous formation of MIFCSN6 complexes confirmed that the JAMM motif and JAMM-mediated isopeptidase activity [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

No evidence text available

reach
"35 S–MPN coimmunoprecipitated with rMIF was then revealed by SDS–PAGE electrophoresis in a 18% gel and, upon drying of the gel, exposure of an X-ray screen.Binding of MIF to CSN6 was examined by CoIP [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In summary, these data showed that the MPN domain independent of its JAMM function is sufficient to mediate MIF–JAB1 interaction in vitro and in vivo, that, on the side of MIF, sequence 50–65 accounts[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
COPS6 affects Neoplasms
| 2 12
| 2 12

reach
"With cell confluence assay and colony formation assay, we find that knockdown (KD) of CSN6 significantly inhibited tumor growth in HCC cell line in vitro (Figure S1D–F, Supporting Information)."

reach
"These data suggested that CSN6‐FOXO4 axis deregulation could exist in many types of cancer.In our cancer model study, CSN6 overexpression promoted cancer growth while CSN6 knockdown inhibited tumor growth in xenograft colon cancer model."

reach
"CSN6 mediates nucleotide metabolism to promote tumor development and chemoresistance in colorectal cancer."

reach
"CSN6 increased the tumor growth rate and tumor weight compared with vector control-transfected cells (Figure 6A-C)."

reach
"In this regard, Zou et al. reported that CSN6 mediates nucleotide metabolism to promote tumor development and chemoresistance in colorectal cancer (CRC) [85]."

reach
"More CSN6 positive signals and fewer p16 signals were observed in CSN6-overexpressing tumors than in the control group (Figure 6E)."

reach
"Both COPS6 and COPS9 knockdown also reduced viable HCC cell number after several days in culture as estimated by CCK8 assay, while overexpression significantly increased (Figure 11B), suggesting that COPS6 and COPS9 accelerate HCC tumor growth."

eidos
"It has been reported that CSN6 can promote some degree of tumor progression by regulating the stability of several E3 ligases6-8 ,21 ."

eidos
"COPS6 promotes tumor progression and reduces CD8 + T cell infiltration by repressing IL-6 production to facilitate tumor immune evasion in breast cancer ."

reach
"A colitis-associated colorectal cancer model established that Csn6 intestinal conditional deletion decreased tumor development and altered nucleotide metabolism."
COPS6 affects MIF
1 | 4 10
1 | 4 10

sparser
"For comparison of MIFCSN6 with MIF–JAB1 binding, CoIPs of endogenous MIF–JAB1 complexes were performed from HEK293mycJAB1 cells by a similar protocol, except that the mouse monoclonal anti-JAB1 antib[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Comparison with a CoIP of endogenous MIF–JAB1 complexes revealed that formation of the MIFCSN6 complexes was slightly weaker than that of the MIF–JAB1 complexes."

sparser
"Since CSN6 does not contain a functional JAMM motif within its MPN domain ( Fig. 4 A), endogenous formation of MIFCSN6 complexes confirmed that the JAMM motif and JAMM-mediated isopeptidase activity [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Since the MIFCSN6 interaction is somewhat weaker than that between MIF and JAB1/CSN5, it may be implied that JAB1 is the major interaction partner of MIF in the cell, but that additional weaker inter[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The potential interaction between MIF and CSN6 was examined by CoIP between endogenous MIF and endogenous CSN6 using an anti-CSN6 antibody for CoIP."

reach
"Comparison with a CoIP of endogenous MIF–JAB1 complexes revealed that formation of the MIFCSN6 complexes was slightly weaker than that of the MIF–JAB1 complexes."

reach
"Since CSN6 does not contain a functional JAMM motif within its MPN domain ( Fig. 4 A), endogenous formation of MIFCSN6 complexes confirmed that the JAMM motif and JAMM-mediated isopeptidase activity [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

No evidence text available

reach
"35 S–MPN coimmunoprecipitated with rMIF was then revealed by SDS–PAGE electrophoresis in a 18% gel and, upon drying of the gel, exposure of an X-ray screen.Binding of MIF to CSN6 was examined by CoIP [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In summary, these data showed that the MPN domain independent of its JAMM function is sufficient to mediate MIF–JAB1 interaction in vitro and in vivo, that, on the side of MIF, sequence 50–65 accounts[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
TRIM21 affects COPS6
| 4 10
| 4 10

sparser
"Notably, studies have unveiled intriguing interactions, for instance, PKMYT1's physical interaction with β-catenin, which stabilizes β-catenin protein and activates Wnt signaling, bolstering non-small cell lung cancer CSC self-renewal. xref Additionally, the CSN6-TRIM21 axis has surfaced, spotlighting its role in driving cancer stemness during tumorigenesis."

sparser
"Besides, the signaling axis of CSN6-TRIM21 was reported to promote tumor stemness during tumorigenesis ( xref )."

reach
"To verify Cullin 's involvement, we first showed that TRIM21 interacted with CUL1 and CSN6 by Co-IP."

sparser
"TRIM21 binding with CSN6 is enhanced by the presence of CUL1 (Fig.  xref )."

reach
"TRIM21 binding with CSN6 is enhanced by the presence of CUL1."

sparser
"In addition, TRIM21’s function is associated with autoimmune diseases, such as systemic lupus erythematosus (SLE) and Sjögren’s syndrome. xref , xref Notably, patients with SLE or Sjögren’s syndrome have an increased risk for developing certain cancers, including non-Hodgkin’s lymphoma. xref In addition, TRIM21 interacts with endoglin, xref which is a prognostic marker in CRC xref and also act as a CSC marker in renal cell carcinoma. xref Future studies will need to address how the CSN6TRIM21 axis may impact these signalling pathways to promote tumorigenesis."

sparser
"This finding aligns with a previous report that the CSN6-TRIM21 axis drives colorectal cancer stemness [ xref ]."

sparser
"In conclusion, we validate a pathway for cancer stemness regulation involving ALDH1A1 levels through the CSN6TRIM21 axis, which may be utilised as CRC molecular markers and be targeted for therapeutic intervention in cancers."

sparser
"Then we confirmed that CSN6 could interact with TRIM21 in HCT116 cells by Co-IP (Fig.  xref )."

sparser
"The MPN domain (41–174 aa) is located in N-terminal and is indispensable in CSN6’s impact on Cullin-1 neddylation. xref Then we further identified the domain of CSN6 responsible for its interaction with TRIM21 and found that the MPN domain of CSN6 bound to TRIM21 (Fig.  xref )."
NPM1 affects COPS6
1 | 13
NPM1 binds COPS6.
1 | 12
1 | 12

sparser
"Indeed, three proteins involved in gemcitabine drug resistance have been characterized in our CSN6NPM1 axis."

No evidence text available

sparser
"Collectively, these results indicate that the CSN6NPM1 axis and downstream ribosome biogenesis are augmented in gemcitabine‐resistant PDAC cells, potentially contributing to gemcitabine resistance."

sparser
"NSC348884, a small‐molecule inhibitor targeting NPM1, disrupts its biological function via oligomerization interference (Figure xref , Supporting Information). [ xref , xref ] To assess the therapeutic potential of targeting the CSN6NPM1 axis for suppressing PDAC progression and overcoming gemcitabine resistance, we therefore treated PDAC cell lines with NSC348884."

sparser
"Conversely, pharmacological inhibition of the CSN6NPM1 axis suppresses tumor growth and alleviates gemcitabine resistance."

sparser
"To determine the clinical significance of targeting the CSN6NPM1 axis with NSC348884, we subcutaneously implanted CSN6‐OE BXPC‐3 cells into nude mice and administered gemcitabine or NSC348884 via intraperitoneal injection every other day."

sparser
"In conclusion, these data demonstrate that gemcitabine‐NSC348884 combination suppresses the CSN6NPM1 regulatory axis and may serve as a promising therapeutic strategy for PDAC patients with high CSN6 expression."

sparser
"Targeting the CSN6NPM1 signaling axis by combined‐treatment with gemcitabine and an NPM1 inhibitor (NSC348884) improves the therapeutic efficacy of gemcitabine in CSN6‐high PDAC."

sparser
"CSN6 Binds to NPM1 and Stabilizes it by Modulating Ubiquitination, Thereby Promoting NPM1‐Mediated Ribosome Biogenesis and Cell Growth."

sparser
"In conclusion, our results demonstrate a CSN6NPM1 link in PDAC progression."
NPM1 ubiquitinates COPS6.
| 1
NPM1 ubiquitinates COPS6. 1 / 1
| 1

sparser
"Interestingly, although CSN6 KD increased NPM1 ubiquitination, this effect was attenuated upon expression of a His‐tagged K48R ubiquitin mutant, suggesting the involvement of K48‐linked polyubiquitination (Figure  xref )."
COPS6 affects Snail1
| 14
COPS6 activates Snail1.
| 7
COPS6 activates Snail1. 7 / 7
| 7

reach
"We then validated whether ubiquitination is involved in CSN6 mediated Snail1 regulation."

reach
"The above results suggest that CSN6 promotes Snail1 stability via impeding ubiquitination of Snail1."

reach
"On the basis of our data, it is possible that CSN6 mediated Snail1 stabilization may be a common feature of various types of cancer."

reach
"CSN6 promotes the cell migration of breast cancer cells by positively regulating Snail1 stability."

reach
"Ubiquitination assay was performed to validate whether ubiquitination is involved in the upregulation of Snail1 by CSN6."

reach
"Taken together, these results indicated that CSN6 promotes Snail1 stability through reducing ubiquitination."

reach
"Our study showed that CSN6 prevented Snail1 degradation via proteasomal degradation."
COPS6 binds Snail1.
| 3
COPS6 binds Snail1. 3 / 3
| 3

reach
"Co-immunoprecipitation study was used to show the interaction between the protein CSN6 and Snail1."

reach
"We next investigated how CSN6 interacted with Snail1 to regulate the protein level of Snail1."

reach
"Western Blot analysis showed that CSN6 could up-regulate the expression of Snail1 protein in a dose dependent manner, and CO-IP analysis showed that CSN6 could bind with Snail1."
COPS6 deubiquitinates Snail1.
| 2
COPS6 leads to the deubiquitination of Snail1. 2 / 2
| 2

reach
"CSN6 induces EMT and enhances metastasis of breast cancer cells by reducing Snail1 ubiquitination."

reach
"CSN6 probably functions to inhibit Snail1 ubiquitination and then switches the E3 ligase target from Snail1 itself to other proteins."
COPS6 decreases the amount of Snail1.
| 2
Modified COPS6 decreases the amount of Snail1. 2 / 2
| 2

reach
"Ubiquitination assay was performed and revealed that CSN6 overexpression decreased the ubiquitination level of Snail1."

reach
"Then we performed ubiquitination assay and found that CSN6 overexpression decreased the ubiquitination level of Snail1."
FOXO4 affects COPS6
2 | 2 9
FOXO4 binds COPS6.
2 | 1 9
2 | 1 9

sparser
"Results showed that FOXO4 bound to the C‐terminus of CSN6 (aa 185‐327 containing) but not to the N‐terminus (aa 1‐184, containing the MPN domain; Figure  xref )."

sparser
"Significantly, as the CSN6FOXO4 axis is crucial in regulating gene expression of Glut1 and SGOC genes, these genes were reduced in CSN6 knockdown tumors (Figure S17E, Supporting Information)."

sparser
"Together, the regulatory circuit of CSN6FOXO4 axis could be recapitulated in mouse xenograft cancer model, and level of FOXO4 deregulation plays roles in affecting the outcome of tumorigenicity."

sparser
"These data suggested that CSN6FOXO4 axis deregulation could exist in many types of cancer."

sparser
"CSN6FOXO4 Axis Is Critical for Tumorigenicity in Xenograft Mouse Model."

sparser
"Deregulation of CSN6FOXO4 Axis Rewires Metabolic Programming via Enhancing Glucose Uptake and Promotes the Expression of SGOC Genes."

sparser
"We further confirmed that CSN6FOXO4 link could affect cell proliferation."

sparser
"As CSN6FOXO4 axis impacts on the expression of Glut1, biochemical assays that quantitates the glucose uptake (consumption) by assessing uptake of (2‐( N ‐(7‐nitrobenz‐2‐oxa‐1,3‐diazol‐4‐yl)amino)‐2‐deoxyglucose (2‐NBDG)), a green fluorescent glucose analog, additionally demonstrated that CSN6 knockdown inhibited 2‐NBDG uptake (Figure  xref ), while FOXO4 knockdown increased 2‐NBDG uptake (Figure  xref )."

reach
"Mechanistic studies show that CSN6 binds and regulates FOXO4 stability through enhancing the E3 ligase activity of COP1, and that COP1 directly interacts with FOXO4 through a VP motif on FOXO4 and accelerates the ubiquitin-mediated degradation of FOXO4."

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FOXO4 inhibits COPS6.
| 1
FOXO4 inhibits COPS6. 1 / 1
| 1

reach
"Importantly, EGF‐mediated down regulation of FOXO4 can be reversed by the knockdown of CSN6 (Figure 1D), suggesting the involvement of CSN6 during this process."
COPS7B affects COPS6
13 |
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COPS6 affects NPM1
1 | 12
1 | 12

sparser
"Indeed, three proteins involved in gemcitabine drug resistance have been characterized in our CSN6NPM1 axis."

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sparser
"Collectively, these results indicate that the CSN6NPM1 axis and downstream ribosome biogenesis are augmented in gemcitabine‐resistant PDAC cells, potentially contributing to gemcitabine resistance."

sparser
"NSC348884, a small‐molecule inhibitor targeting NPM1, disrupts its biological function via oligomerization interference (Figure xref , Supporting Information). [ xref , xref ] To assess the therapeutic potential of targeting the CSN6NPM1 axis for suppressing PDAC progression and overcoming gemcitabine resistance, we therefore treated PDAC cell lines with NSC348884."

sparser
"Conversely, pharmacological inhibition of the CSN6NPM1 axis suppresses tumor growth and alleviates gemcitabine resistance."

sparser
"To determine the clinical significance of targeting the CSN6NPM1 axis with NSC348884, we subcutaneously implanted CSN6‐OE BXPC‐3 cells into nude mice and administered gemcitabine or NSC348884 via intraperitoneal injection every other day."

sparser
"In conclusion, these data demonstrate that gemcitabine‐NSC348884 combination suppresses the CSN6NPM1 regulatory axis and may serve as a promising therapeutic strategy for PDAC patients with high CSN6 expression."

sparser
"Targeting the CSN6NPM1 signaling axis by combined‐treatment with gemcitabine and an NPM1 inhibitor (NSC348884) improves the therapeutic efficacy of gemcitabine in CSN6‐high PDAC."

sparser
"CSN6 Binds to NPM1 and Stabilizes it by Modulating Ubiquitination, Thereby Promoting NPM1‐Mediated Ribosome Biogenesis and Cell Growth."

sparser
"In conclusion, our results demonstrate a CSN6NPM1 link in PDAC progression."
COPS6 affects COPS7B
13 |
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CUL1 affects COPS6
8 1 | 2 1
8 1 | 2 1

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sparser
"These findings highlight the complexity of TRIM21 regulation and indicate that CSN6-CUL1 regulation is involved in TRIM21 ubiquitination."

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reach
"First, we found that CSN6 associated with Myc, Fbxw7 and Cullin-1 in U2OS cells in vivo (XREF_FIG), suggesting that CSN6 interacts with SCF complex and may regulate it to stabilize Myc."

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reach
"We found that both CSN6 and CSN5 associated with Cullin-1 and that CSN6 and CSN5 competed with each other for Cullin-1 binding in a dose dependent manner (XREF_FIG)."

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DDB2 affects COPS6
11 |
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CUL4B affects COPS6
11 |
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CUL3 affects COPS6
11 |
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COPS6 affects FOS
1 | 6 4
COPS6 binds FOS.
1 | 2 4
1 | 2 4

sparser
"Thirdly, CSN6 interacted with c-Fos and decreased its ubiquitination directly, thereby attenuating the degradation of c-Fos ( Fig. 6 )."

No evidence text available

reach
"Both COP9 and c-Fos have been described as nuclear proteins, so we analyzed the subcellular interaction between CSN6 and c-Fos by western blotting of the nucleus fractions."

reach
"Our co-IP also showed a direct interaction between CSN6 and c-Fos in nucleus ( Fig. 6 e), and they are also interacted with each other in other three cell lines (data not show)."

sparser
"Thus, we first observed whether there was interaction between the CSN6 protein and c-Fos protein."

sparser
"Both COP9 and c-Fos have been described as nuclear proteins, so we analyzed the subcellular interaction between CSN6 and c-Fos by western blotting of the nucleus fractions."

sparser
"Our co-IP also showed a direct interaction between CSN6 and c-Fos in nucleus ( Fig. 6 e), and they are also interacted with each other in other three cell lines (data not show)."
COPS6 deubiquitinates FOS.
| 3
COPS6 deubiquitinates FOS. 3 / 3
| 3

reach
"Since CSN6 has the activity of deubiquitinase, so we next asked whether CSN6 deubiquitinates c-Fos."

reach
"We analyzed c-Fos ubiquitination in the presence of MG132 and found that MG132-induced c-Fos ubiquitination was abolished by overexpression of CSN6, on the contrary, knocking down of CSN6 increase the[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"In summary, Our results demonstrated that CSN6 inhibited ubiquitination of c-Fos via its deubiquitination function, thus stabilized c-Fos protein.The main causes of cancer-related death are metastasis[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
COPS6 activates FOS.
| 1
COPS6 activates FOS. 1 / 1
| 1

reach
"On the contrary, overexpression of CSN6 increased c-Fos's protein expression and AP-1 transcriptional activity ( Fig. S9 a-c)."
COPS6 affects DDB2
11 |
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COPS6 affects CUL4B
11 |
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COPS6 affects CUL3
11 |
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UBE3A affects COPS6
2 | 5
2 | 5

sparser
"We characterized that CSN6 associated with E6AP and stabilized E6AP expression by reducing E6AP poly-ubiquitination, thereby regulating p53 activity in cell proliferation and apoptosis."

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sparser
"CSN6 interacts with E6AP in vivo and causes E6AP stabilization."

sparser
"These results indicate that CSN6 associates with E6AP and that E6AP can positively affect the steady-state expression of CE6AP."

sparser
"We found that CSN6 associates with E6AP and reduces E6AP autoubiquitination, thereby stabilizing E6AP."

sparser
"Our data show that N-terminal CSN6 is interacting with E6AP."
COPS6 affects autophagy
| 10
| 10

reach
"CSN6 promotes the migration and invasion of CC cells through inhibiting autophagy."

reach
"We found that knockdown of CSN6 induced increased fluorescent protein mRFP+-GFP+-LC3 puncta, suggesting that CSN6 knockdown elicits autophagy flux in CC cells (Fig. 5D)."

reach
"CSN6 inhibits autophagy by increasing mTOR expression in CC cells."

reach
"In rice blast fungus, Csn6 deletion leads to enhanced autophagy, which is manifested by an increased number of autophagosomes and an accelerated autophagy rate [26]."

reach
"These results suggested that CSN6 could inhibit autophagy by increasing mTOR expression in CC cells."

reach
"Knockdown of CSN6 induced appropriate autophagy pathways and significantly increased LC3-II accumulation in our study.In recent years, autophagy has been reported to be associated with CTSL 26, 31, 32."

reach
"Interestingly, our results indicated that autophagy is essential for decreasing CTSL, while CSN6 could inhibit the autophagy ability of CC cells."

reach
"Knockdown of CSN6 enhances autophagy in CC cells."

reach
"In addition, blocking of the mammalian target of rapamycin (mTOR) pathway reversed CSN6-mediated autophagy inhibition."

reach
"Endogenous LC3-II turnover blocked by autophagy inhibitor Chloroquine indicated that knockdown of CSN6 induced the normal flux of complete autophagy."
COPS6 affects Ubiquitin
| 10
COPS6 inhibits Ubiquitin.
| 8
| 8

reach
"Study in colorectal cancer has found that up-regulation of CSN6 by EGF decreases SCF ubiquitin ligase substrate receptor β-transduction repeat-containing protein (β-TrCP) by promoting its autocatalyti[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Furthermore, CSN6 has been found to decrease β-TrCP by promoting its autocatalytic degradation, thereby stabilizing SCF ubiquitin ligase substrate in colorectal cancer cells [22] ."

reach
"2.1 EGF Signal and CSN6/COP1 Enhance Ubiquitin-Mediated Degradation of FOXO4."

reach
"Indeed, we revealed that CSN6 reduces the stability of UBR5 by regulating the ubiquitin mediated degradation of UBR5, furtherly stabilizing CDK9 expression, suggesting that UBR5 may be a downstream factor of CSN6 in melanoma cells."

reach
"These observations demonstrated that CSN6 might promote the 20S proteasomal ubiquitin-independent degradation of p16."

reach
"We have shown that CSN6 promotes ubiquitin-dependent degradation of TRIM21, and it is then possible that CSN6 can regulate FASN stability through TRIM21 as well."

reach
"Taken together, these results indicate that EGF signaling elevated CSN6 and COP1 to cause down regulation of FOXO4 regardless of its PKB/Akt‐mediated phosphorylation status.2.2 CSN6 Enhances Ubiquitin-Mediated Degradation of FOXO4 through K48 Link."

reach
"These data suggested that CSN6 attenuates ubiquitin-mediated degradation of FASN, thereby stabilizing FASN protein."
COPS6 activates Ubiquitin.
| 2
| 2

reach
"For example, CSN6, a component of the COP9 signalosome, positively regulate E3 ubiquitin ligases."

reach
"31 Our data indicates that CSN6 enhances ubiquitin‐mediated degradation of FOXO4 through K48 link ubiquitination, thereby downregulating FOXO4.2.3 COP1 Is Involved in CSN6-Mediated Ubiquitination of FOXO4."
COPS6 affects UBR5
| 1 8 1
COPS6 inhibits UBR5.
| 1 3
COPS6 inhibits UBR5. 4 / 4
| 1 3

eidos
"Next , western blot analysis demonstrated that CSN6 increased CDK9 expression and decreased UBR5 expression in a dose-dependent manner in 293FT cells ( Fig. 5B ) ."

reach
"This study demonstrated that UBR5, a novel E3 ubiquitin ligase interacting with CSN6, was negatively regulated by CSN6 and was responsible for CDK9 ubiquitination and degradation in melanoma cells."

reach
"Indeed, we revealed that CSN6 reduces the stability of UBR5 by regulating the ubiquitin mediated degradation of UBR5, furtherly stabilizing CDK9 expression, suggesting that UBR5 may be a downstream factor of CSN6 in melanoma cells."

reach
"In addition, our study described a novel CSN6 interacting E3 ligase UBR5, which was negatively regulated by CSN6 and could regulate the ubiquitination and degradation of CDK9 in melanoma cells."
COPS6 decreases the amount of UBR5.
| 2
COPS6 decreases the amount of UBR5. 2 / 2
| 2

reach
"Furthermore, we found that UBR5 knockdown rescued all the effects induced by CSN6 silencing, indicating that CSN6 activates the CDK9 pathway to promote melanoma growth and metastasis by reducing the UBR5 level."

reach
"Next, western blot analysis demonstrated that CSN6 increased CDK9 expression and decreased UBR5 expression in a dose dependent manner in 293FT cells."
COPS6 binds UBR5.
| 1 1
COPS6 binds UBR5 and A375. 1 / 1
| 1

reach
"The E3 ubiquitin ligase UBR5 has shown the ability to ubiquitinate CDK9 38, so we hypothesized that CSN6 stabilizes the expression of CDK9 by regulating the E3 ligase UBR5.A co-IP assay was performed and demonstrated that CSN6 interacted with UBR5 in A375 and MV3 melanoma cells."
| 1

sparser
"A co-IP assay was performed and demonstrated that CSN6 interacted with UBR5 in A375 and MV3 melanoma cells (Fig. xref )."
COPS6 activates UBR5.
| 2
COPS6 activates UBR5. 2 / 2
| 2

reach
"Then, western blot analysis showed that the proteasome inhibitor MG132 could rescue CSN6 mediated UBR5 downregulation."

reach
"Furthermore, in CSN6-knockdown melanoma cells, UBR5 knockdown abrogated the effects caused by CSN6 silencing, suggesting that CSN6 activates the UBR5 and CDK9 pathway to promote melanoma cell proliferation and metastasis."
COPS6 affects UBE3A
2 | 5
2 | 5

sparser
"We characterized that CSN6 associated with E6AP and stabilized E6AP expression by reducing E6AP poly-ubiquitination, thereby regulating p53 activity in cell proliferation and apoptosis."

No evidence text available

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sparser
"CSN6 interacts with E6AP in vivo and causes E6AP stabilization."

sparser
"These results indicate that CSN6 associates with E6AP and that E6AP can positively affect the steady-state expression of CE6AP."

sparser
"We found that CSN6 associates with E6AP and reduces E6AP autoubiquitination, thereby stabilizing E6AP."

sparser
"Our data show that N-terminal CSN6 is interacting with E6AP."
COPS6 affects SNAI1
2 | 2 6
COPS6 binds SNAI1.
2 | 6
2 | 6

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sparser
"Co-immunoprecipitation study was used to show the interaction between the protein CSN6 and Snail1."

sparser
"Also we showed that CSN6 associates with Snail1 and enhances Snail1 protein level by inhibiting the ubiquitin-mediated degradation of Snail1."

sparser
"Conclusion: These findings provide new insight into applicability of using the CSN6-Snail1 axis as a potential therapeutic target in breast cancer."

sparser
"Our findings provided a thoughtful understanding of novel CSN6-Snail1 signaling in promoting breast cancer metastasis."

sparser
"The results of co-immunoprecipitation showed that CSN6 and Snail1 proteins bound to each other (Figure xref D)."

sparser
"Western Blot analysis showed that CSN6 could up-regulate the expression of Snail1 protein in a dose-dependent manner, and CO-IP analysis showed that CSN6 could bind with Snail1."
COPS6 activates SNAI1.
| 2
COPS6 activates SNAI1. 2 / 2
| 2

eidos
"Thus , we speculated that CSN6 induced up-regulation of Snail may be regulated at the post-transcriptional level ."

eidos
"Ubiquitination assay was performed to validate whether ubiquitination is involved in the upregulation of Snail1 by CSN6 ."
COPS6 affects DCAF1
7 | 3
COPS6 binds DCAF1.
7 | 1
7 | 1

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sparser
"Co‐IP experiments confirmed that CSN6 interacts with DCAF1, and molecular docking predicted a structural model for their interaction (Figure  xref and Figure xref , Supporting Information)."

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COPS6 ubiquitinates DCAF1.
| 2
COPS6 ubiquitinates DCAF1. 2 / 2
| 2

sparser
"Since DCAF1 is a component of Cullin4‐Ring Ligases (CRL4) complexes, we hypothesized that CSN6 might promote DCAF1 ubiquitination through Cullin4."

sparser
"Indeed, Cullin4 KD attenuated CSN6 OE‐mediated DCAF1 ubiquitination (Figure  xref ), suggesting that CSN6‐induced ubiquitination of DCAF1 is Cullin4‐dependent."
COPS6 affects CC
| 10
COPS6 activates CC.
| 6
COPS6 activates CC. 6 / 6
| 6

reach
"CSN6 promotes CC cells migration and invasion."

reach
"Furthermore, our results found that up-regulation of CSN6 increased the wound closure ability of CC cells (Fig. 2G)."

reach
"The contrary result was got in CSN6 overexpressioned CC cells migration and invasion assay (Fig. 2I)."

reach
"Our data demonstrated that CSN6 could promote metastasis of CC cells via CTSL."

reach
"Our results demonstrated that CSN6 could promote metastasis of CC cells via CTSL."

reach
"CSN6 promotes the migration and invasion of CC cells through inhibiting autophagy."
COPS6 inhibits CC.
| 4
COPS6 inhibits CC. 4 / 4
| 4

reach
"Interestingly, our results indicated that autophagy is essential for decreasing CTSL, while CSN6 could inhibit the autophagy ability of CC cells."

reach
"We found that knockdown of CSN6 induced increased fluorescent protein mRFP+-GFP+-LC3 puncta, suggesting that CSN6 knockdown elicits autophagy flux in CC cells (Fig. 5D)."

reach
"To assess the metastatic effects of CSN6 in CC, we performed transwell assay and found that down-regulation of CSN6 strongly decreased the migration and invasion abilities of CC cells (Fig. 2H)."

reach
"Knockdown of CSN6 enhances autophagy in CC cells."
EGF affects COPS6
| 9
EGF increases the amount of COPS6.
| 4
EGF increases the amount of COPS6. 4 / 4
| 4

reach
"Immunoblot analysis showed that EGF treatment increased the steady-state expression of CSN6 in DLD-1 colon cancer cells, whereas inhibition of MEK and ERK by the MEK1 inhibitor PD98059 diminished the EGF induced increase of CSN6 expression (XREF_FIG)."

reach
"Furthermore, we found that EGF treatment increased the S60 phosphorylation level of CSN6 with concurrent Akt phosphorylation."

reach
"Treatment with calf intestinal alkaline phosphatase (CIP) reduced the EGF induced steady-state expression of CSN6, indicating that CSN6 phosphorylation is required in this process (XREF_FIG) and presumably acts through ERK."

reach
"Immunoblotting analysis showed that EGF treatment decreased the steady‐state expression of FOXO4 within 45 min (Figure  1A, Figure S1A, Supporting Information) and accelerated turnover rate of FOXO4 (Figure S1B, Supporting Information) in several CRC cell lines, whereas the EGF induced the expression of CSN6 and COP1, an E3 ligase, within that period of time (Figure 1A, Figure S1A, Supporting Information)."
EGF activates COPS6.
| 3
EGF activates COPS6. 3 / 3
| 3

reach
"Importantly, EGF‐mediated down regulation of FOXO4 can be reversed by the knockdown of CSN6 (Figure 1D), suggesting the involvement of CSN6 during this process."

reach
"The CSN6 S148A mutant did not respond to EGF induced upregulation, whereas the WT CSN6 was upregulated by EGF."

reach
"EGF-mediated upregulation of FASN can be compromised by the CSN6 KD, suggesting the important role of CSN6 in this process (Fig. 8b)."
EGF decreases the amount of COPS6.
| 2
EGF decreases the amount of COPS6. 2 / 2
| 2

reach
"Furthermore, we found that EGF treatment or ERK2 activation decreases the ubiquitination level of CSN6 (XREF_FIG)."

reach
"We assessed the ubiquitination of CSN6 in the presence of EGF and found that EGF reduced the ubiquitination level of WT CSN6 but not that of the CSN6 S148A mutant (XREF_FIG)."
DCAF11 affects COPS6
9 |
9 |

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2 3 1 | 2 1
COPS6 binds.
3 1 |
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COPS6 translocates.
| 2 1
COPS6 translocates to the nucleus. 2 / 2
| 1 1

sparser
"Human Vpr interacting protein (hVIP/Mov34), which binds to both Vpr and GR, translocates to the nucleus following either dexamethasone or Vpr treatment, further suggesting that Vpr and GR form an functional complex within cells [ xref ]."

reach
"Human Vpr interacting protein (hVIP and Mov34), which binds to both Vpr and GR, translocates to the nucleus following either dexamethasone or Vpr treatment, further suggesting that Vpr and GR form an functional complex within cells [XREF_BIBR]."
COPS6 translocates from the cytoplasm to the nucleus. 1 / 1
| 1

reach
"XREF_BIBR Overexpression of Akt translocates CSN6 from the cytoplasm to the nucleus, which could facilitate its effects on MDM2."
COPS6 is active.
2 |
COPS6 phosphorylated on S60 is active. 2 / 2
2 |

"Mechanistic studies show that akt causes csn6 phosphorylation at ser 60, which, in turn, reduces ubiquitin-mediated protein degradation of csn6."

"Mechanistic studies show that akt causes csn6 phosphorylation at ser 60, which, in turn, reduces ubiquitin-mediated protein degradation of csn6."
COPS6 affects fbxw1
| 7 2
COPS6 binds fbxw1.
| 2 2
| 2 2

reach
"We found that CSN6 also associates with beta-Trcp and facilitates its degradation."

sparser
"We found that CSN6 also associates with β-Trcp and facilitates its degradation."

sparser
"We found that CSN6 associated with β-Trcp in vivo ( xref ), suggesting that CSN6 interacts with F-box protein β-Trcp and regulates it, thereby stabilizing β-catenin."

reach
"We found that CSN6 associated with beta-Trcp in vivo (XREF_FIG), suggesting that CSN6 interacts with F-box protein beta-Trcp and regulates it, thereby stabilizing beta-catenin."
COPS6 activates fbxw1.
| 3
COPS6 activates fbxw1. 3 / 3
| 3

reach
"qRT-PCR analysis revealed that the mRNA levels of beta-Trcp and beta-catenin were not affected by CSN6 overexpression (XREF_SUPPLEMENTARY), and western blotting revealed that CSN6 mediated beta-Trcp downregulation could be rescued by the proteasome inhibitor MG132 (XREF_FIG)."

reach
"CSN6 overexpression reduced the turnover rate of beta-catenin and increased the turnover rate of beta-Trcp (XREF_FIG)."

reach
"Western blotting revealed that the CSN6 mediated beta-Trcp downregulation in K1 cells could be rescued by the proteasome inhibitor MG132."
COPS6 inhibits fbxw1.
| 2
COPS6 inhibits fbxw1. 2 / 2
| 2

reach
"Because Cullin neddylation, a process controlled by CSN, decreases the steady-state expression levels of F-box proteins, we rationalized that CSN6 may be involved in Cullin neddylation, thereby increasing the autocatalytic degradation of beta-Trcp."

reach
"As expected, we found that the beta-catenin level decreased and that of beta-Trcp increased when cells were treated with CSN6-shRNA (CSN6 knockdown) (Fig XREF_FIG A and B)."
COPS6 affects TP53
1 1 | 7
COPS6 inhibits TP53.
| 5
COPS6 inhibits TP53. 5 / 5
| 5

reach
"Through combinatorial effects on the MDM2-p53 signaling axis and now SKP2 mediated p57 Kip2 degradation, it is hypothesized that CSN6 overexpression can effectively promote cell proliferation by simultaneously relieving cell cycle restraints, apoptosis, senescence, and presumably p53 functions in DNA damage repair and cell metabolism, among other important tumor suppressive mechanisms."

reach
"Moreover, CSN6 promotes p53 degradation through a mechanism that stabilizes MDM2 protein by limiting its auto-ubiquitylation."

reach
"Similarly, it was found that over-expression of CSN6 can promote p53 degradation through inhibiting autoubiquitylation of MDM2, and mice that were heterozygous for CSN6 were more susceptible to DNA damage [XREF_BIBR]."

reach
"Moreover, CSN6 could n't induce p53 degradation in Mdm2-null mouse embryonic fibroblasts, suggesting that CSN6 mediated degradation of p53 is MDM2 dependent."

reach
"These results suggest that loss of CSN6 enhances p53 mediated tumor suppression in vivo and that CSN6 plays an important role in regulating DNA damage associated apoptosis and tumorigenesis through control of the MDM2-p53 signaling pathway."
COPS6 binds TP53.
1 1 |
1 1 |

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COPS6 activates TP53.
| 2
COPS6 activates TP53. 2 / 2
| 2

reach
"Since 14-3-3sigma is regulated by p53, at issue is whether CSN6 mediated 14-3-3sigma downregulation involves p53."

reach
"Noticeably, reduced expression of Csn6 in Emicro-Myc background can also contribute to p53 stabilization in 33% of Emicro-Myc and Csn6 +/- lymphomas when compared with Emicro-Myc and Csn6 +/+, suggesting that both CSN6 mediated p53 downregulation and CSN6 mediated Myc elevation contribute to lymphomagenesis of this system (XREF_FIG)."
COPS6 affects HYCC1
| 9
COPS6 activates HYCC1. 9 / 9
| 9

reach
"Recent research by Li et al. demonstrated that signalosome 6 (CSN6) levels are elevated in HCC and act as an activator of hydroxymethylglutaryl-CoA synthase 1 (HMGCS1) in the mevalonate pathway, promoting the development of tumors [127]."

reach
"Here it is shown that CSN6 is elevated in HCC and is a positive regulator of hydroxymethylglutaryl-CoA synthase 1 (HMGCS1) of mevalonate (MVA) pathway to promote tumorigenesis."

reach
"Together, these results suggested that high CSN6 expression is important for HCC growth and correlates with poor survival.2.2 CSN6 Promotes YAP1 Activation in HCC."

reach
"We demonstrate that CSN6 is elevated in HCC and correlates with poor progonosis."

reach
"Both COPS6 and COPS9 knockdown also reduced viable HCC cell number after several days in culture as estimated by CCK8 assay, while overexpression significantly increased (Figure 11B), suggesting that COPS6 and COPS9 accelerate HCC tumor growth."

reach
"CSN6 promotes HCC cancer cell growth in vitro and in vivo through YAP1 nuclear translocalization and activation."

reach
"Both COPS6 and COPS9 overexpression promoted HCC cell migration and Matrigel invasion in transwell assays, while COPS6 siRNA transfection or COPS9 siRNA transfection repressed transwell migration and Matrigel invasion (Figure 11C)."

reach
"In HCC, CSN6 overexpression promotes epithelial-mesenchymal transition and predicts poor prognosis (26)."

reach
"With cell confluence assay and colony formation assay, we find that knockdown (KD) of CSN6 significantly inhibited tumor growth in HCC cell line in vitro (Figure S1D–F, Supporting Information)."
COPS6 affects HMGCS1
| 9
COPS6 ubiquitinates HMGCS1.
| 4
COPS6 leads to the ubiquitination of HMGCS1. 4 / 4
| 4

reach
"Importantly, CSN6 KD efficiently increased the polyubiquitination level of HMGCS1 (Figure 4C), while CSN6 overexpression reduced HMGCS1 polyubiquitination (Figure 4D)."

reach
"Under physiological condition, Csn6 liver cancer tissue demonstrated higher SPOP expression, less expression of MDM2, HMGCS1 and survivin (YAP1 target gene) when compared with Csn6 liver cancer tissue (Figure 6J,K), suggesting that CSN6‐MDM2 axis‐mediated SPOP ubiquitination and consequential HMGCS1 stabilization can be recapitulated in Csn6 mouse liver cancer.2.7 Targeting CSN6-HMGCS1 Axis to Suppress Tumor Growth in NAFLD Related HCC."

reach
"Taken together, these data showed that CSN6HMGCS1 axis regulates mevalonate metabolism, promoting YAP1 nuclear translocation and transcriptional activity to facilitate tumor progression.2.4 CSN6 Promotes HMGCS1 Stabilization by Attenuating HMGCS1 Ubiquitination."

reach
"Thus, CSN6 antagonizes SPOP‐mediated HMGCS1 polyubiquitination.2.6 CSN6-MDM2 Axis-Mediated SPOP Ubiquitination."
COPS6 deubiquitinates HMGCS1.
| 2
COPS6 leads to the deubiquitination of HMGCS1. 2 / 2
| 2

reach
"Importantly, CSN6 KD efficiently increased the polyubiquitination level of HMGCS1 (Figure 4C), while CSN6 overexpression reduced HMGCS1 polyubiquitination (Figure 4D)."

reach
"Thus, CSN6 antagonizes SPOP‐mediated HMGCS1 polyubiquitination.2.6 CSN6-MDM2 Axis-Mediated SPOP Ubiquitination."
COPS6 activates HMGCS1.
| 2
COPS6 activates HMGCS1. 2 / 2
| 2

reach
"Overexpression of CSN6 leads to a slower turnover rate of HMGCS1, while CSN6 KD accelerated the turnover rate of HMGCS1 (Figure 4E–G)."

reach
"With IP‐mass spectrometry and quantitative proteomics assay, we identified 68 potential CSN6 direct targets and found that CSN6 directly regulates HMGCS1 protein stability."
COPS6 decreases the amount of HMGCS1.
| 1
COPS6 decreases the amount of HMGCS1. 1 / 1
| 1

reach
"13 To confirm the proteomic results, we found that CSN6 KD in liver cancer led to reduced steady‐state expression of HMGCS1 (Figure S3C, Supporting Information)."
COPS6 affects DCAF11
9 |
9 |

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COPS6 affects AKT
| 5 3
COPS6 activates AKT.
| 5 2
COPS6 activates AKT. 7 / 8
| 5 2

reach
"Consistent with this, we found that CSN6 can downregulate the expression of 14-3-3sigma and lead to Akt activation."

sparser
"In addition to demonstrating amplification of the CSN6 gene locus at 7q22.1 in human mammary tumors, xref they report that AKT phosphorylation at Ser60 stabilizes CSN6. xref Interestingly, by promoting 14–3-3σ degradation through stabilization of its E3-ligase COP1, CSN6 can activate AKT to create a self-propelling positive feedback loop xref ( xref )."

reach
"The observation that CSN6 increases the activity of Akt (XREF_FIG) is very intriguing."

reach
"Our mechanistic studies of CSN6 mediated 14-3-3sigma downregulation explains how CSN6 can activate Akt in our proposed model (XREF_FIG)."

reach
"XREF_BIBR Interestingly, by promoting 14-3-3sigma degradation through stabilization of its E3-ligase COP1, CSN6 can activate AKT to create a self propelling positive feedback loop XREF_BIBR (XREF_FIG)."

sparser
"Our mechanistic studies of CSN6-mediated 14-3-3σ downregulation explains how CSN6 can activate Akt in our proposed model ( xref )."

reach
"Because 14-3-3sigma suppresses Akt activity, we first examined whether knockdown of CSN6 could suppress Akt activity and inhibit Akt mediated cell survival."
COPS6 binds AKT.
| 1
| 1

sparser
"Akt is able to associate with CSN6 and phosphorylate it at Ser60, which can reduce the ubiquitin-mediated protein degradation and turnover rate of CSN6, thereby increasing steady-state expression of CSN6."
CDK9 affects COPS6
| 4 5
| 4 5

reach
"This study demonstrates that CSN6 interacts with CDK9 and increases the stability of CDK9 by controlling the expression of the E3 ligase UBR5."

reach
"CSN6 interacts with CDK9 and regulates CDK9 stability."

reach
"In summary, these results indicate that CSN6 interacts with CDK9 and regulates CDK9 stability by reducing CDK9 ubiquitination and degradation."

sparser
"This study demonstrates that CSN6 interacts with CDK9 and increases the stability of CDK9 by controlling the expression of the E3 ligase UBR5."

sparser
"CSN6 interacts with CDK9 and regulates CDK9 stability."

sparser
"A co-IP assay further validated that CSN6 interacted with CDK9 in A375 and MV3 melanoma cells (Fig. xref )."

reach
"Furthermore, we revealed that CSN6 interacted with CDK9 by co-IP."

sparser
"In summary, these results indicate that CSN6 interacts with CDK9 and regulates CDK9 stability by reducing CDK9 ubiquitination and degradation."

sparser
"Furthermore, we revealed that CSN6 interacted with CDK9 by co-IP."
SNAI1 affects COPS6
2 | 6
2 | 6

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sparser
"Co-immunoprecipitation study was used to show the interaction between the protein CSN6 and Snail1."

sparser
"Also we showed that CSN6 associates with Snail1 and enhances Snail1 protein level by inhibiting the ubiquitin-mediated degradation of Snail1."

sparser
"Conclusion: These findings provide new insight into applicability of using the CSN6-Snail1 axis as a potential therapeutic target in breast cancer."

sparser
"Our findings provided a thoughtful understanding of novel CSN6-Snail1 signaling in promoting breast cancer metastasis."

sparser
"The results of co-immunoprecipitation showed that CSN6 and Snail1 proteins bound to each other (Figure xref D)."

sparser
"Western Blot analysis showed that CSN6 could up-regulate the expression of Snail1 protein in a dose-dependent manner, and CO-IP analysis showed that CSN6 could bind with Snail1."
DCAF1 affects COPS6
7 | 1
7 | 1

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sparser
"Co‐IP experiments confirmed that CSN6 interacts with DCAF1, and molecular docking predicted a structural model for their interaction (Figure  xref and Figure xref , Supporting Information)."

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CUL5 affects COPS6
7 1 |
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CUL2 affects COPS6
8 |
8 |

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eidos
"CSN6 induces EMT and enhances metastasis of breast cancer cells by reducing Snail1 ubiquitination ."

reach
"Together, the results suggest that CSN6 may positively regulate beta-catenin transcription, thereby mediating the EMT."

reach
"Additionally, CSN6 was found to promote EMT by inhibiting E-cadherin, which were significantly mitigated via upregulation of Snail as a result of MEK and ERK pathway activation."

reach
"In addition, studies shown that CSN6 has the function of deubiquitinase and promoted tumorigenesis, proliferation, metastasis and EMT by inhibiting substrates degradation [ 14–16 ]."

reach
"CSN6 promotes PTC progression by inducing the EMT."

reach
"In HCC, CSN6 overexpression promotes epithelial-mesenchymal transition and predicts poor prognosis (26)."
COPS6 increases the amount of epithelial to mesenchymal transition.
| 2

reach
"CSN6 positively regulates beta-catenin expression in a beta-Trcp-dependent manner and triggers the expression of several EMT related genes regulated by beta-catenin."

reach
"CSN6 positively regulated beta-catenin expression in a beta-Trcp-dependent manner and triggered expression of several EMT related genes regulated by beta-catenin."
COPS6 affects MTOR
| 8
COPS6 activates MTOR.
| 4
COPS6 activates MTOR. 4 / 4
| 4

reach
"The data showed that CSN6-mediated mTOR up-regulation was enhanced by the 26S proteasome inhibitor MG132 (Fig. 7A)."

reach
"This indicated that CSN6 increased mTOR stability in a proteasome-dependent manner."

reach
"Furthermore, CSN6 decreased the turnover rate of mTOR in the presence of CHX, a de novo protein synthesis inhibitor (Fig. 7B)."

reach
"Our data showed that CSN6-mediated mTOR up-regulation was enhanced by the 26S proteasome inhibitor MG132."
COPS6 ubiquitinates MTOR.
| 2
COPS6 leads to the ubiquitination of MTOR. 2 / 2
| 2

reach
"CSN6 increases mTOR stability by reducing mTOR ubiquitination."

reach
"Our findings showed that CSN6 could increase mTOR stability by reducing mTOR ubiquitination."
COPS6 decreases the amount of MTOR.
| 2
COPS6 decreases the amount of MTOR. 2 / 2
| 2

reach
"To validate whether ubiquitination is involved in CSN6-mediated stabilization of mTOR, we then performed ubiquitination assay and found that overexpression of CSN6 decreased the ubiquitination level of mTOR (Fig. 7C and D)."

reach
"Ubiquitination assay indicated that overexpression of CSN6 decreased the ubiquitination level of mTOR."
COPS6 affects MDM2
| 8
COPS6 deubiquitinates MDM2.
| 3
COPS6 leads to the deubiquitination of MDM2 on K364. 2 / 2
| 2

reach
"Mechanism studies indicated that CSN6 prevented MDM2 autoubiquitination at lysine 364, resulting in stabilization of MDM2 and degradation of p53."

reach
"Previous studies have demonstrated that CSN6 prevents MDM2 autoubiquitination at lysine 364, which results in the stabilization of MDM2 and the degradation of p53."
Modified COPS6 leads to the deubiquitination of MDM2 on K364. 1 / 1
| 1

reach
"Expression of CSN6 appeared to prevent MDM2 autoubiquitination at lysine 364, resulting in stabilization of MDM2 and degradation of p53."
COPS6 activates MDM2.
| 3
COPS6 activates MDM2. 3 / 3
| 3

reach
"CSN6 enhances MDM2 stabilization and plays an essential role in promoting tumorigenesis by regulating MDM2/p53 signaling ."

reach
"CSN6 may promote carcinogenesis by positively regulating v-myc avian myelocytomatosis viral oncogene homolog (Myc) and MDM2 proto-oncogene stability, and is regarded as a potential target for cancer therapy."

reach
"Previously, Lee 's laboratory demonstrated that CSN6, a subunit of the COP9 signalosome, interacts with and inhibits the degradation of an oncogene MDM2, leading to its stabilization (XREF_FIG)."
COPS6 binds MDM2.
| 2
| 2

reach
"Similarly, overexpression of COPS6 binds to MDM2, and prevents the process of its auto-ubiquitination and degradation, which leads to the accumulation of MDM2 and decrease of p53 in breast cancer (49)."

reach
"CSN6 makes a contribution to the binding of CSN and E3 ligases [9,10] , and directly binds to MDM2, which is an ubiquitin ligases correlated with p53 pathway [11] ."
COPS6 affects MAPK1
2 | 4 2
2 | 2

sparser
"Mechanistic studies have shown that CSN6 is deregulated by EGFR signaling, in which ERK2 interacts with CSN6."

sparser
"In CRC, CSN6 was deregulated by EGFR signaling, in which ERK2 bound directly to CSN6 Leu163/Val165 and phosphorylated CSN6 at Ser148."

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MAPK1 binds COPS6 and Leu163. 2 / 2
| 2

reach
"Mechanistic studies suggested that ERK2 directly binds to CSN6 Leu163/Val165 and phosphorylates COPS6 at Ser148, thereby regulating β-Trcp and stabilizing β-catenin expression, consequently blocking the ubiquitin-proteasome pathway and promoting CRC development.Programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) checkpoint blockade is an emerging immunotherapy modality in various tumors, while its regulatory mechanism remains uncertain."

reach
"Mechanistic studies revealed that CSN6 is deregulated by EGFR signaling, in which ERK2 binds directly to CSN6 Leu163 and Val165 and phosphorylates CSN6 at Ser148."
MAPK1 binds COPS6 and Ser148. 2 / 2
| 2

reach
"Mechanistic studies suggested that ERK2 directly binds to CSN6 Leu163/Val165 and phosphorylates COPS6 at Ser148, thereby regulating β-Trcp and stabilizing β-catenin expression, consequently blocking the ubiquitin-proteasome pathway and promoting CRC development.Programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) checkpoint blockade is an emerging immunotherapy modality in various tumors, while its regulatory mechanism remains uncertain."

reach
"Mechanistic studies revealed that CSN6 is deregulated by EGFR signaling, in which ERK2 binds directly to CSN6 Leu163 and Val165 and phosphorylates CSN6 at Ser148."
COPS6 affects MAP3K1
3 | 1 1 1
COPS6 binds MAP3K1.
3 | 1 1
3 | 1 1

sparser
"Here, we show that CSN6 associates with MEKK1 and reduces MEKK1 expression level by facilitating the ubiquitin-mediated degradation of MEKK1."

trips
"Here, we show that CSN6 associates with MEKK1 and reduces MEKK1 expression level by facilitating the ubiquitin-mediated degradation of MEKK1."

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COPS6 decreases the amount of MAP3K1.
| 1
COPS6 decreases the amount of MAP3K1. 1 / 2
| 1

reach
"Here, we show that CSN6 associates with MEKK1 and reduces MEKK1 expression level by facilitating the ubiquitin mediated degradation of MEKK1."
| 1 6
| 1 6

eidos
"CSN6 promotes GBM proliferation , migration , invasion , and tumorigenesis through upregulation of EGFR by blocking its ubiquitination ; this happens as a result of interactions with CHIP that cause its degradation , although CHIP auto-ubiquitination occurs through an unknown mechanism [ 91 ] ."

reach
"In addition to the ubiquitin-dependent degradation of proteins, Jab1/CSN5 and CSN6 promote tumorigenesis via ubiquitin independent proteasomal degradation of the cell cycle regulators p16 and p14ARF ."

reach
"In addition, studies shown that CSN6 has the function of deubiquitinase and promoted tumorigenesis, proliferation, metastasis and EMT by inhibiting substrates degradation [ 14–16 ]."

reach
"9e Besides, we also found that CSN6 promotes tumorigenesis in mice through directly stabilization of MDM2."

reach
"For example, tumorigenesis is caused by mutations of CSN5 and CSN6."

reach
"Abnormalities in the appeal pathway may be the main cause of 5-FU resistance, for example, a recent study by Zou et al. found that CSN6 promotes colorectal carcinogenesis and chemotherapy tolerance by mediating nucleotide metabolism ."

reach
"CSN6 promotes tumorigenesis of gastric cancer by ubiquitin-independent proteasomal degradation of p16 INK4a ."
COPS6 affects CUL5
7 1 |
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COPS6 affects CUL2
8 |
8 |

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COPS6 affects COP1
5 |
5 |

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CDKN2A affects COPS6
2 | 3 3
CDKN2A binds COPS6.
2 | 2 3
2 | 2 3

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sparser
"Our study provides the in vitro and in vivo evidence that CSN6-p16 serves as an important target for GC therapy."

sparser
"Immunofluorescence localization and a co-immunoprecipitation study were used to show the interaction between the protein CSN6 and p16."

reach
"Immunofluorescence localization and a co-immunoprecipitation study were used to show the interaction between the protein CSN6 and p16."

reach
"In addition, co-IP showed association between endogenous CSN6 and p16 in MKN-45 and MGC-803 cells."

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sparser
"Our mechanistic studies indicated that CSN6 interacted with p16, and that CSN6-mediated p16 downregulation was suppressed by a proteasome inhibitor."
CDKN2A increases the amount of COPS6.
| 1
CDKN2A increases the amount of COPS6. 1 / 1
| 1

reach
"As we invalidated before, CSN6 promoted the cell proliferation and cell clone formation, and the restoration of p16 rescued the phenotypes of CSN6 overexpression (Figure 3D-3E)."
CDKN1B affects COPS6
1 | 1 2 3
1 | 1 2 3

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sparser
"Mechanistic studies showed that CSN6 interacts with p27 and enhances ubiquitin-mediated degradation of p27."

trips
"Mechanistic studies show that CSN6 interacts with p27(Kip1) and facilitates ubiquitin-mediated degradation of p27(Kip1)."

reach
"Mechanistic studies show that CSN6 interacts with p27 (Kip1) and facilitates ubiquitin mediated degradation of p27 (Kip1)."

sparser
"Mechanistic studies show that CSN6 interacts with p27(Kip1) and facilitates ubiquitin-mediated degradation of p27(Kip1)."

reach
"Mechanistic studies show that CSN6 interacts with p27 and facilitates ubiquitin mediated degradation of p27."

sparser
"Mechanistic studies show that CSN6 interacts with p27 and facilitates ubiquitin-mediated degradation of p27."
SKP2 affects COPS6
4 | 2
4 | 2

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reach
"XREF_BIBR The authors provide evidence that CSN6 interacts with SKP2 to promote p57 Kip2 polyubiquitylation."

reach
"Mechanism studies show that CSN6 interacts with p57 and Skp2 through its C-terminal domain, which, in turn, promotes Skp2 mediated protein ubiquitination of p57, thereby decreasing the steady-state expression of p57."

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SIRT2 affects COPS6
| 4 3
SIRT2 binds COPS6.
| 1 3
| 1 3

sparser
"Thus, we initially detected whether there was an interaction between CSN6 and SIRT2."

sparser
"Co-immunoprecipitation (co-IP) result suggested that there was no interaction between CSN6 and SIRT2 under the treatment of Ang II ( Fig.S3 A )."

reach
"Thus, we initially detected whether there was an interaction between CSN6 and SIRT2."

sparser
"But we found that there was no interaction between CSN6 and SIRT2."
SIRT2 inhibits COPS6.
| 2
SIRT2 inhibits COPS6. 2 / 2
| 2

reach
"As shown in Fig. 3 F–H, knocking down the expression of SIRT2 exacerbated the Ang II-induced myocardial hypertrophy, more important, the inhibition of SIRT2 could reverse the decrease of cardiomyocyte[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"29 The latest research showed that constitutive photomorphogenic 9 signalosome complex subunit 6 (CSN6) and plant homeodomain finger protein 19 (PHF19) exacerbate the pathological myocardial hypertrophy induced by Ang II by inhibiting the expression of SIRT2, and the overexpression of SIRT2 can reduce the effect of CSN6 in promoting myocardial hypertrophy."
SIRT2 activates COPS6.
| 1
SIRT2 activates COPS6. 1 / 1
| 1

reach
"In the present study, we found that overexpression of CSN6 inhibited the mRNA and protein expression of SIRT2, and SIRT2 mediated the pro-hypertrophic function of CSN6 in cardiomyocytes response to An[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
RBX1 affects COPS6
2 1 | 3 1
2 1 | 3

reach
"CSN6 processing occurs in CSN-CRL complexes and is followed by the cleavage of Rbx1, the direct interaction partner of CSN6."

reach
"Furthermore, ZK287.5 interacted with CSN-1 and CSN-6 in two-hybrid assays CUL-3 Regulation by Neddylation and Deneddylation 917Figure 5."

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reach
"In addition, components of signaling pathways such as IkappaBalpha 17 and the adenomatous polyposis coli (APC) protein 18 are stabilized by USP15.In CSN-CRL complexes, subunit CSN2 interacts with cull[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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Csn binds CSN2, COPS6, CUL, and RBX1. 1 / 1
| 1

sparser
"CSN physically interacts with the cullin and RBX1 subunits of CRLs through its subunits CSN2 and CSN6 and it is thought that these interactions provide CSN with the affinity for its CRL targets ( xref )."
FOS affects COPS6
1 | 2 4
1 | 2 4

sparser
"Thirdly, CSN6 interacted with c-Fos and decreased its ubiquitination directly, thereby attenuating the degradation of c-Fos ( Fig. 6 )."

No evidence text available

reach
"Both COP9 and c-Fos have been described as nuclear proteins, so we analyzed the subcellular interaction between CSN6 and c-Fos by western blotting of the nucleus fractions."

reach
"Our co-IP also showed a direct interaction between CSN6 and c-Fos in nucleus ( Fig. 6 e), and they are also interacted with each other in other three cell lines (data not show)."

sparser
"Thus, we first observed whether there was interaction between the CSN6 protein and c-Fos protein."

sparser
"Both COP9 and c-Fos have been described as nuclear proteins, so we analyzed the subcellular interaction between CSN6 and c-Fos by western blotting of the nucleus fractions."

sparser
"Our co-IP also showed a direct interaction between CSN6 and c-Fos in nucleus ( Fig. 6 e), and they are also interacted with each other in other three cell lines (data not show)."
ERK affects COPS6
| 3 4
ERK binds COPS6.
| 1 3
| 1 3

sparser
"In the present study, replacing some conserved residues on the D- site compromised CSN6’s capacity to interact with ERK, suggesting that these residues are involved in ERK-CSN6 protein-protein interaction."

reach
"A coimmunoprecipitation assay revealed that ERK1/2 binds to CSN6 (XREF_FIG)."

sparser
"A coimmunoprecipitation assay revealed that ERK1/2 binds to CSN6 ( xref )."

sparser
"Model of CSN6-ERK Interaction."
ERK phosphorylates COPS6.
| 2 1
ERK phosphorylates COPS6. 3 / 3
| 2 1

reach
"Recently, phosphorylation of CSN6 by ERK was found to stabilize beta-catenin for colon cancer cell proliferation."

sparser
"Recently, phosphorylation of CSN6 by ERK was found to stabilize β-catenin for colon cancer cell proliferation ( xref )."

reach
"Accordingly, CSN6 S148A was resistant to phosphorylation by recombinant ERK, whereas WT CSN6 was highly phosphorylated by ERK, in an in vitro kinase assay (XREF_FIG)."
ERCC8 affects COPS6
7 |
7 |

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DDB1 affects COPS6
7 |
7 |

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| 1 6
| 1 6

reach
"Also, WT CSN6 increased cell migration and invasion in a wound healing assay (p < 0.001, XREF_FIG) and in transwell migration and invasion assays (XREF_FIG), whereas CSN6 S148A lost such capability, demonstrating that the CSN6 promoted migration and invasion of colon cancer cells requires a phosphorylation event."

reach
"Furthermore, migration and invasion assays showed that knockdown of CSN6 reduced cell migration and invasion in DLD-1."

reach
"CSN6 promotes the cell migration of breast cancer cells by positively regulating Snail1 stability."

reach
"CSN6 promoted the cell migration and wound healing abilities in breast cancer cell lines."

reach
"Here, we identified that CSN6 promoted melanoma cell migration and invasion in vivo."

eidos
"These results indicate that CSN6 affects the EMT process of cancer cells by up-regulating the expression of Snail1 protein and promotes cell migration ."

reach
"Both COPS6 and COPS9 overexpression promoted HCC cell migration and Matrigel invasion in transwell assays, while COPS6 siRNA transfection or COPS9 siRNA transfection repressed transwell migration and Matrigel invasion (Figure 11C)."
COPS6 affects SPOP
| 7
COPS6 inhibits SPOP.
| 4
COPS6 inhibits SPOP. 4 / 4
| 4

reach
"Mechanistically, CSN6 antagonizes speckle-type POZ protein (SPOP) ubiquitin ligase to stabilize HMGCS1, which in turn activates YAP1 to promote tumor growth."

reach
"In the diethylnitrosamine plus CCl mouse model, CSN6 antagonizes speckle-type POZ protein (SPOP) and stabilizes hydroxymethylglutaryl-CoA synthase 1 (HMGCS1), resulting in YAP activation through the mevalonate pathway."

reach
"CSN6 negatively regulates SPOP to stabilize HMGCS1, which activates YAP via mevalonate metabolism."

reach
"All these data suggested that CSN6 promotes SPOP degradation via E3 ligase MDM2."
COPS6 ubiquitinates SPOP.
| 2
COPS6 leads to the ubiquitination of SPOP. 2 / 2
| 2

reach
"Under physiological condition, Csn6 liver cancer tissue demonstrated higher SPOP expression, less expression of MDM2, HMGCS1 and survivin (YAP1 target gene) when compared with Csn6 liver cancer tissue (Figure 6J,K), suggesting that CSN6‐MDM2 axis‐mediated SPOP ubiquitination and consequential HMGCS1 stabilization can be recapitulated in Csn6 mouse liver cancer.2.7 Targeting CSN6-HMGCS1 Axis to Suppress Tumor Growth in NAFLD Related HCC."

reach
"Thus, CSN6 antagonizes SPOP‐mediated HMGCS1 polyubiquitination.2.6 CSN6-MDM2 Axis-Mediated SPOP Ubiquitination."
COPS6 deubiquitinates SPOP.
| 1
COPS6 leads to the deubiquitination of SPOP. 1 / 1
| 1

reach
"With ubiquitination assay, CSN6 KD decreased ubiquitination of SPOP (Figure 6F)."
COPS6 affects SKP2
4 | 2
4 | 2

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reach
"XREF_BIBR The authors provide evidence that CSN6 interacts with SKP2 to promote p57 Kip2 polyubiquitylation."

reach
"Mechanism studies show that CSN6 interacts with p57 and Skp2 through its C-terminal domain, which, in turn, promotes Skp2 mediated protein ubiquitination of p57, thereby decreasing the steady-state expression of p57."

No evidence text available

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COPS6 affects RBX1
2 1 | 3 1
2 1 | 3

reach
"CSN6 processing occurs in CSN-CRL complexes and is followed by the cleavage of Rbx1, the direct interaction partner of CSN6."

reach
"Furthermore, ZK287.5 interacted with CSN-1 and CSN-6 in two-hybrid assays CUL-3 Regulation by Neddylation and Deneddylation 917Figure 5."

No evidence text available

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reach
"In addition, components of signaling pathways such as IkappaBalpha 17 and the adenomatous polyposis coli (APC) protein 18 are stabilized by USP15.In CSN-CRL complexes, subunit CSN2 interacts with cull[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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Csn binds CSN2, COPS6, CUL, and RBX1. 1 / 1
| 1

sparser
"CSN physically interacts with the cullin and RBX1 subunits of CRLs through its subunits CSN2 and CSN6 and it is thought that these interactions provide CSN with the affinity for its CRL targets ( xref )."
COPS6 affects FOXA1
| 4 3
COPS6 binds FOXA1.
| 1 3
| 1 3

sparser
"To assess the clinical relevance of the CSN6-FOXA1 regulatory axis in human PAAD progression, we analyzed the association between the expression levels of CSN6 and FOXA1 in 107 PAAD patients."

sparser
"Thus, we firstly observed whether there was interaction between CSN6 and FOXA1."

sparser
"As showed in Fig. S6 a, co-immunoprecipitation(co-IP) result suggested that there was no interaction between CSN6 protein and FOXA1 protein( Fig.S8 a )."

reach
"Thus, we firstly observed whether there was interaction between CSN6 and FOXA1."
COPS6 increases the amount of FOXA1.
| 3
COPS6 increases the amount of FOXA1. 3 / 3
| 3

reach
"Silencing CSN6 caused a decrease of mRNA and protein expression of FOXA1, and overexpression of CSN6 upregulated mRNA and protein expression of FOXA1 ( Fig. 3 a and b)."

reach
"The result showed that inhibition of CSN6 suppressed protein expression of c-Fos but had no influence on mRNA expression of c-Fos, and decreased mRNA and protein expression of FOXA1 ( Fig. 5 a and b)."

reach
"Overexpression of CSN6 could upregulate c-Fos protein thereby enhancing AP-1 transcriptional activity to promote mRNA expression of FOXA1 ( Fig. 5 )."
COPS6 affects ERCC8
7 |
7 |

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COPS6 affects EGFR
| 5
COPS6 increases the amount of EGFR.
| 1
COPS6 increases the amount of EGFR. 1 / 3
| 1

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"Moreover, CSN6 decreased CHIP expression and increased EGFR expression in the tumor samples."
COPS6 activates EGFR.
| 2
COPS6 activates EGFR. 2 / 2
| 2

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"Our studies showed that CSN6-FASN axis increase palmitate production, implying that CSN6 may increase EGFR palmitoylation and possibly causes TKI-drug resistance."

reach
"Moreover, CSN6 positively regulates EGFR stability, thereby promoting glioblastoma proliferation and metastasis ."
COPS6 deubiquitinates EGFR.
| 1
COPS6 leads to the deubiquitination of mutated EGFR. 1 / 1
| 1

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"Reduction of mutant EGFR ubiquitination by CSN6 causes steadily elevated levels of EGFR, leading to proliferation, migration, invasion, and tumorigenesis 35."
COPS6 decreases the amount of EGFR.
| 1
COPS6 decreases the amount of EGFR. 1 / 1
| 1

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"Moreover, CSN6 decreased CHIP expression and increased EGFR expression in the tumor samples."
COPS6 affects DDB1
7 |
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COPS6 affects CUL
| 3 3
COPS6 binds CUL.
| 1 3
| 1 1

reach
"On the other hand, it is conceivable that in CSN6 overexpressing cancers abundant CSN6 binds more amounts of Cullin and thus reduces the accessibility of Cullin to CSN5 for deneddylation, thereby preserving Cullin neddylation."

sparser
"Indeed, based on the mole fraction of Csn6 that is bound to individual cullins, up to 60% of CSN remains associated with cullins following immunoprecipitation ( xref )."
CSN2 binds RING-box protein 1, CUL, and COPS6. 1 / 1
| 1

sparser
"The CSN becomes assembled in supercomplexes with CRLs in which CSN2 interacts with cullins and CSN6 with Rbx1 [16,17] ."
Csn binds CSN2, COPS6, CUL, and RBX1. 1 / 1
| 1

sparser
"CSN physically interacts with the cullin and RBX1 subunits of CRLs through its subunits CSN2 and CSN6 and it is thought that these interactions provide CSN with the affinity for its CRL targets ( xref )."
COPS6 activates CUL.
| 2
COPS6 activates CUL. 2 / 2
| 2

reach
"We find that CSN6 enhanced Cullin neddylation facilitates auto-ubiquitination and degradation of Myc E3 ligase Fbxw7, thereby stabilizing Myc, indicates that CSN6 is a positive upstream regulator of Myc."

reach
"CSN6 competes with CSN5 to increase Cullin neddylation."
COPS6 affects COPS6
| 7
COPS6 activates COPS6.
| 4
COPS6 activates COPS6. 4 / 4
| 4

reach
"In addition, phosphorylation of CSN6 increases the stability of CSN6, thereby promoting its regulatory capacity."

reach
"The EGF-ERK2 Axis Phosphorylates CSN6 on S148 and Enhances CSN6 Stabilization."

reach
"CSN6 S60 phosphorylation is known to increase the stability of CSN6 [XREF_BIBR]."

reach
"CSN6 mRNA levels did not increase significantly in response to EGF (XREF_SUPPLEMENTARY), suggesting that EGF regulates CSN6 posttranslationally."
COPS6 inhibits COPS6.
| 3
COPS6 inhibits COPS6. 3 / 3
| 3

reach
"Mechanistic studies revealed that CSN6 is deregulated by EGFR signaling, in which ERK2 binds directly to CSN6 Leu163 and Val165 and phosphorylates CSN6 at Ser148."

reach
"Fig. 4 B indicates that PDGF significantly increased the protein level of CSN6 to 2.71-fold over control ( P < 0.05), while pre-treatment of cells with imatinib reduced PDGF-induced the increase of CS[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"As depicted in Fig. 3 A, PDGF significantly increased the protein level of CSN6 to 2.21-fold compared with control ( P < 0.05), while pre-treatment of cells with LY294002 reduced CSN6 protein level to[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
COPS6 affects BTBD2
6 1 |
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PDGF affects COPS6
| 6
PDGF increases the amount of COPS6.
| 3
PDGF increases the amount of COPS6. 3 / 3
| 3

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"Fig. 2 B shows that PDGF increased the protein level of CSN6 in a time-dependent manner, and peak time for 10 ng/ml PDGF up-regulation of CSN6 was 10 min, suggesting that CSN6 is up-regulated in PASMC[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"As depicted in Fig. 3 A, PDGF significantly increased the protein level of CSN6 to 2.21-fold compared with control ( P < 0.05), while pre-treatment of cells with LY294002 reduced CSN6 protein level to[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Fig. 4 B indicates that PDGF significantly increased the protein level of CSN6 to 2.71-fold over control ( P < 0.05), while pre-treatment of cells with imatinib reduced PDGF-induced the increase of CS[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
PDGF activates COPS6.
| 3
PDGF activates COPS6. 3 / 3
| 3

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"These results suggest that PDGFR mediates PDGF-induced up-regulation of CSN6 in PASMCs.Next, we examined whether activation of PDGFR mediates down-regulation of β-TrCP in PDGF-stimulated PASMCs."

reach
"Fig. 2 B shows that PDGF increased the protein level of CSN6 in a time-dependent manner, and peak time for 10 ng/ml PDGF up-regulation of CSN6 was 10 min, suggesting that CSN6 is up-regulated in PASMC[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"These results indicate that PDGFR mediate PI3K/Akt signaling pathway activation in PASMCs treated with PDGF.To examine whether activation of PDGFR mediates PDGF-induced up-regulation of CSN6 in PASMCs[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
NEDD8 affects COPS6
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MAP3K1 affects COPS6
3 | 1 1
3 | 1 1

sparser
"Here, we show that CSN6 associates with MEKK1 and reduces MEKK1 expression level by facilitating the ubiquitin-mediated degradation of MEKK1."

trips
"Here, we show that CSN6 associates with MEKK1 and reduces MEKK1 expression level by facilitating the ubiquitin-mediated degradation of MEKK1."

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LRR1 affects COPS6
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DCAF4 affects COPS6
6 |
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COPS6 affects cell cycle
| 6
COPS6 activates cell cycle.
| 4
| 4

reach
"We demonstrated that COPS6 and COPS9 promote cell cycle progression, but it is unknown whether these effects are dependent on p21 and p18 degradation via a ubiquitin-mediated process."

reach
"Taken together, these data demonstrated that CSN6 could promote cell cycle progression in GC cells."

reach
"To explore the molecular mechanism underlying CSN6-induced cell cycle progression, we measured the expression of some G1 cell cycle regulatory proteins."

reach
"This study revealed that CSN6 knockdown in melanoma cells inhibited cell proliferation in vitro and tumorigenicity in mice by inducing cell cycle arrest and that the anti-proliferative effect could be rescued by overexpressing CSN6 in CSN6-knockdown melanoma cells, suggesting that CSN6 has critical roles in triggering melanoma initiation and progression."
COPS6 inhibits cell cycle.
| 2
| 2

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"Through combinatorial effects on the MDM2-p53 signaling axis and now SKP2 mediated p57 Kip2 degradation, it is hypothesized that CSN6 overexpression can effectively promote cell proliferation by simultaneously relieving cell cycle restraints, apoptosis, senescence, and presumably p53 functions in DNA damage repair and cell metabolism, among other important tumor suppressive mechanisms."

reach
"As the cellular abundance of p27 is an indicator of cell cycle, we thus reviewed relative articles to find whether the cell cycle arrest effect caused by COPS5 or COPS6 inhibition was p27 dependent."
COPS6 affects SLC22A1
| 6
COPS6 deubiquitinates SLC22A1.
| 3
COPS6 leads to the deubiquitination of SLC22A1. 3 / 3
| 3

reach
"Moreover, overexpression of CSN6 reduced OCT1 ubiquitination, while ectopic expression of TRIM21 reversed this effect."

reach
"Simultaneously, knockdown of CSN6 increased OCT1 ubiquitination, while knockdown of TRIM21 reversed this impact."

reach
"In colorectal cancer, COP9 signalosome subunit 6 (CSN6) facilitates ubiquitin-mediated tripartite -motif protein 21 (TRIM21) degradation, reducing TRIM21-mediated organic cation transporter subtypes 1 (OCT1) ubiquitination, stabilizing OCT1, and promoting CSCs [94]."
COPS6 decreases the amount of SLC22A1.
| 2
COPS6 decreases the amount of SLC22A1. 2 / 2
| 2

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"Consistent with this finding, we found that knockdown of CSN6 increased the ubiquitination level of OCT1."

reach
"We found that overexpression of CSN6 reduced the ubiquitination level of OCT1."
COPS6 ubiquitinates SLC22A1.
| 1
COPS6 leads to the ubiquitination of SLC22A1. 1 / 1
| 1

reach
"In colorectal cancer, COP9 signalosome subunit 6 (CSN6) facilitates ubiquitin-mediated tripartite -motif protein 21 (TRIM21) degradation, reducing TRIM21-mediated organic cation transporter subtypes 1 (OCT1) ubiquitination, stabilizing OCT1, and promoting CSCs [94]."
COPS6 affects NEDD8
6 |
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COPS6 affects LRR1
6 |
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| 3 3
| 3

sparser
"This shuttling of p27 Kip1 from the nucleus to the cytoplasm could be mediated by additional interaction with CSN6 and the E3 ubiquitin ligase constitutive photomorphogenic 1 (COP1) [ xref ]."

sparser
"This study demonstrated that UBR5, a novel E3 ubiquitin ligase interacting with CSN6, was negatively regulated by CSN6 and was responsible for CDK9 ubiquitination and degradation in melanoma cells."

sparser
"COPS6 interacts with SCF-type E3 ubiquitin ligases and acts as a positive regulator of E3 ubiquitin ligases involved in various cellular and developmental processes [ 48 , 49 ]."
COPS6 activates E3_Ub_ligase.
| 3
| 3

reach
"It has been reported that CSN6 can promote some degree of tumor progression by regulating the stability of several E3 ligases XREF_BIBR - XREF_BIBR, XREF_BIBR."

reach
"Mechanistic studies show that CSN6 binds and regulates FOXO4 stability through enhancing the E3 ligase activity of COP1, and that COP1 directly interacts with FOXO4 through a VP motif on FOXO4 and accelerates the ubiquitin-mediated degradation of FOXO4."

reach
"For example, CSN6, a component of the COP9 signalosome, positively regulate E3 ubiquitin ligases."
COPS6 affects DCAF4
6 |
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COPS6 affects BTBD1
6 |
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Vpr affects COPS6
2 | 2 1
| 2 1

reach
"Human Vpr interacting protein (hVIP and Mov34), which binds to both Vpr and GR, translocates to the nucleus following either dexamethasone or Vpr treatment, further suggesting that Vpr and GR form an functional complex within cells [XREF_BIBR]."

reach
"We demonstrate direct interactions between the putative ligand hVIP and MOV34 and Vpr in vitro and in vivo."

sparser
"These data illustrate that the carboxyl-terminal domain of hVIP is critical for mediating hVIP-Vpr interaction as well as for its glucocorticoid response."
Vpr binds COPS6. 2 / 2
2 |

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Quercetin affects COPS6
| 4
Quercetin inhibits COPS6.
| 2
| 2

reach
"In another study, quercetin inhibited the viability of HT29 cells, caused cell shrinkage, chromatin condensation, and nuclear collapse, lessened the protein expression levels of phosphorylated-Akt, and augmented the protein degradation of constitutive photomorphogenesis 6 signalosome (CSN6) (Yang et al., 2016)."

reach
"Western blot analysis revealed that quercetin reduced the protein expression levels of phosphorylated-Akt and increased CSN6 protein degradation; therefore, affecting the expression levels of Myc, p53, B-cell lymphoma 2 (Bcl-2) and Bcl-2 associated X protein."
Quercetin decreases the amount of COPS6.
| 2
Quercetin decreases the amount of COPS6. 2 / 2
| 2

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"The present study determined that quercetin may reduce the protein expression levels of CSN6 in HT-29 colon cancer cells and that it may be one of the important targets for quercetin induced apoptosis in HT-29 cells."

reach
"Quercetin induces S-phase cell cycle arrest in HT-29 cells, reducing phosphorylated-AKT, CSN6, Bcl-2 and Myc protein levels."
SFN affects COPS6
3 |
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RHOBTB1 affects COPS6
5 |
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MYC affects COPS6
| 5
MYC activates COPS6.
| 3
MYC activates COPS6. 3 / 3
| 3

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"As expected, Myc mediated transcriptional activation target CDK4 and Myc mediated transcriptional repression targets p27Kip1 and p21Cip1 were deregulated in Emicro-Myc and Csn6 +/- lymphomas compared to Emicro-Myc and Csn6 +/+ lymphomas (XREF_FIG)."

reach
"A recent study also demonstrated that CSN6 contributed to carcinogenesis by positive regulation of Myc stability."

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"CSN6 increase Myc stability by reducing Myc ubiquitination."
MYC binds COPS6.
| 2
| 2

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"These data show that the association between CSN6 and Myc is present in many types of human cancer, suggesting that the positive regulation of Myc by CSN6 may be a clinically relevant regulatory pathway in human cancers (XREF_FIG)."

reach
"First, we found that CSN6 associated with Myc, Fbxw7 and Cullin-1 in U2OS cells in vivo (XREF_FIG), suggesting that CSN6 interacts with SCF complex and may regulate it to stabilize Myc."
Flag affects COPS6
| 5
| 5

sparser
"To test this hypothesis, a strain bearing GFP-Csn5 and Flag-Csn6 was generated, and coimmunoprecipitation (co-IP) assays were conducted."

sparser
"These results show that the CSN complex bear only one copy of Csn6, either a full-length or a truncated form (S6CD); while the MPN − domain fragment of Csn6, when expressed without the S6CD and incapable of integrating into the CSN complex, can still interact with over-stoichiometric amounts of Flag-Csn6 ( xref , xref , xref )."

sparser
"Interestingly, HA-S6MPN, which did not co-IP with endogenous CSN subunits, interacted with ectopically expressed Flag-Csn6 ( xref )."

sparser
"As shown in xref , Flag-Csn6 fusion proteins could be identified in proteins copurified with GFP-Csn5, further confirming the interaction between Csn5 and Csn6."

sparser
"FlagCSN6 was previously described. [ xref ] pCMV5‐Flag‐COP1 was kindly provided by E. Bianchi."
FEM1B affects COPS6
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FBXO17 affects COPS6
5 |
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DDA1 affects COPS6
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CUL affects COPS6
| 1 3
| 1 1

reach
"On the other hand, it is conceivable that in CSN6 overexpressing cancers abundant CSN6 binds more amounts of Cullin and thus reduces the accessibility of Cullin to CSN5 for deneddylation, thereby preserving Cullin neddylation."

sparser
"Indeed, based on the mole fraction of Csn6 that is bound to individual cullins, up to 60% of CSN remains associated with cullins following immunoprecipitation ( xref )."
CSN2 binds RING-box protein 1, CUL, and COPS6. 1 / 1
| 1

sparser
"The CSN becomes assembled in supercomplexes with CRLs in which CSN2 interacts with cullins and CSN6 with Rbx1 [16,17] ."
Csn binds CSN2, COPS6, CUL, and RBX1. 1 / 1
| 1

sparser
"CSN physically interacts with the cullin and RBX1 subunits of CRLs through its subunits CSN2 and CSN6 and it is thought that these interactions provide CSN with the affinity for its CRL targets ( xref )."
CSN2 affects COPS6
| 3 2
| 3

reach
"For example, CSN-1 was able to interact with CSN-2, CSN-4, and CSN-5, while CSN-2 interacted with CSN-1, CSN-3, CSN-4, and CSN-6."

reach
"When taken together with the known crystal structure of the SCF [13] this allows us to speculate that the interaction of CSN2 and CSN6 with the SCF could serve to position the CSN5 subunit such that i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The CSN becomes assembled in supercomplexes with CRLs in which CSN2 interacts with cullins and CSN6 with Rbx1 [16,17]."
CSN2 binds RING-box protein 1, CUL, and COPS6. 1 / 1
| 1

sparser
"The CSN becomes assembled in supercomplexes with CRLs in which CSN2 interacts with cullins and CSN6 with Rbx1 [16,17] ."
Csn binds CSN2, COPS6, CUL, and RBX1. 1 / 1
| 1

sparser
"CSN physically interacts with the cullin and RBX1 subunits of CRLs through its subunits CSN2 and CSN6 and it is thought that these interactions provide CSN with the affinity for its CRL targets ( xref )."
COPS6 affects vpr
2 | 2 1
| 2 1

reach
"Human Vpr interacting protein (hVIP and Mov34), which binds to both Vpr and GR, translocates to the nucleus following either dexamethasone or Vpr treatment, further suggesting that Vpr and GR form an functional complex within cells [XREF_BIBR]."

reach
"We demonstrate direct interactions between the putative ligand hVIP and MOV34 and Vpr in vitro and in vivo."

sparser
"These data illustrate that the carboxyl-terminal domain of hVIP is critical for mediating hVIP-Vpr interaction as well as for its glucocorticoid response."
Vpr binds COPS6. 2 / 2
2 |

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COPS6 affects ptc
| 5
COPS6 activates ptc. 5 / 5
| 5

reach
"These results suggest that CSN6 robustly modulates PTC migration and invasiveness."

reach
"Thus, loss of CSN6 expression inhibits PTC proliferation and migration."

reach
"In vitro and in vivo data showed that loss of CSN6 attenuated cell proliferation, migration, and invasion of PTC cells, confirming the vital function of CSN6 in PTC."

reach
"Second, functional assays showed that CSN6 inhibition significantly reduced both the motility and the proliferation of PTC cells."

reach
"CSN6 promotes PTC progression by inducing the EMT."
COPS6 affects cell growth
| 1 3
| 1 3

reach
"MTT and BrdU assays revealed that recovery of CSN6 expression rescued cell growth and proliferation."

eidos
"CSN6 knockdown hindered cell growth , soft agar colony formation , and tumorigenicity ( Figure S17A-D , Supporting Information ) compared with control cells ."

reach
"Also, CSN6 overexpression leads to increased cell growth, transformation and promotes tumorigenicity."

reach
"Knockdown of COPS5 and COPS6 inhibited cell growth and migration of the CAL27 and SCC25 cell lines."
COPS6 affects YAP1
| 5
COPS6 inhibits YAP1.
| 2
COPS6 inhibits YAP1. 2 / 2
| 2

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"Furthermore, CSN6 KD reduced YAP1 nuclear localization (Figure 2F and Figure S2A, Supporting Information)."

reach
"With transcriptional activity assay, we found that CSN6 ablation repressed YAP1‐TEAD transcriptional activity (Figure 2E)."
COPS6 decreases the amount of YAP1.
| 2
COPS6 decreases the amount of YAP1. 2 / 2
| 2

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"With quantitative PCR assay, we showed that CSN6 ablation attenuates YAP1 target gene expression in CSN6 KD cell line and in liver tissues of Csn6 mice (Figure 2C,D)."

reach
"CSN6 KD represses the expression of YAP/TAZ target genes (Figure  2A)."
COPS6 activates YAP1.
| 1
COPS6 activates YAP1. 1 / 1
| 1

reach
"Together, these results suggested that high CSN6 expression is important for HCC growth and correlates with poor survival.2.2 CSN6 Promotes YAP1 Activation in HCC."
COPS6 affects STUB1
| 1 3 1
COPS6 binds STUB1.
| 1 1 1
| 1 1 1

trips
"We showed that CSN6 associated with CHIP and led to CHIP destabilization by increasing CHIP self-ubiquitination."

reach
"We showed that CSN6 associated with CHIP and led to CHIP destabilization by increasing CHIP self ubiquitination."

sparser
"We showed that CSN6 associated with CHIP and led to CHIP destabilization by increasing CHIP self-ubiquitination."
COPS6 decreases the amount of STUB1.
| 2
COPS6 decreases the amount of STUB1. 2 / 2
| 2

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"Moreover, CSN6 decreased CHIP expression and increased EGFR expression in the tumor samples."

reach
"Inhibition of CSN6 by small interfering RNA decreased PD-L1 expression but also increased CHIP expression in GBM cells."
COPS6 affects SFN
3 |
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COPS6 affects RHOBTB1
5 |
5 |

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COPS6 affects NKX2-2
| 5
COPS6 decreases the amount of NKX2-2.
| 3
COPS6 decreases the amount of NKX2-2. 3 / 3
| 3

reach
"As showed in Fig. 5 H, overexpression of CSN6 reduced the ubiquitination level of Nkx2.2."

reach
"But, inhibition of CSN6 increased the ubiquitination level of Nkx2.2 ( Fig. 5 I )."

reach
"Moreover, we found that CSN6 inhibited SIRT2 expression by stabilizing the expression of Nkx2.2, a transcription factor which suppressed SIRT2 transcription [ 24 ]."
COPS6 deubiquitinates NKX2-2.
| 2
COPS6 leads to the deubiquitination of NKX2-2. 2 / 2
| 2

reach
"Next, we tested whether CSN6 inhibited the ubiquitination of Nkx2.2."

reach
"We pointed out that CSN6 reduced the ubiquitination of transcription factor Nkx2.2 and stabilized its protein expression, thus inhibiting SIRT2 transcription."
COPS6 affects Flag
| 5
| 5

sparser
"To test this hypothesis, a strain bearing GFP-Csn5 and Flag-Csn6 was generated, and coimmunoprecipitation (co-IP) assays were conducted."

sparser
"These results show that the CSN complex bear only one copy of Csn6, either a full-length or a truncated form (S6CD); while the MPN − domain fragment of Csn6, when expressed without the S6CD and incapable of integrating into the CSN complex, can still interact with over-stoichiometric amounts of Flag-Csn6 ( xref , xref , xref )."

sparser
"Interestingly, HA-S6MPN, which did not co-IP with endogenous CSN subunits, interacted with ectopically expressed Flag-Csn6 ( xref )."

sparser
"As shown in xref , Flag-Csn6 fusion proteins could be identified in proteins copurified with GFP-Csn5, further confirming the interaction between Csn5 and Csn6."

sparser
"FlagCSN6 was previously described. [ xref ] pCMV5‐Flag‐COP1 was kindly provided by E. Bianchi."
COPS6 affects FEM1B
5 |
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COPS6 affects FBXO17
5 |
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COPS6 affects DDA1
5 |
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COPS6 affects CSN2
| 3 2
| 3

reach
"For example, CSN-1 was able to interact with CSN-2, CSN-4, and CSN-5, while CSN-2 interacted with CSN-1, CSN-3, CSN-4, and CSN-6."

reach
"When taken together with the known crystal structure of the SCF [13] this allows us to speculate that the interaction of CSN2 and CSN6 with the SCF could serve to position the CSN5 subunit such that i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The CSN becomes assembled in supercomplexes with CRLs in which CSN2 interacts with cullins and CSN6 with Rbx1 [16,17]."
CSN2 binds RING-box protein 1, CUL, and COPS6. 1 / 1
| 1

sparser
"The CSN becomes assembled in supercomplexes with CRLs in which CSN2 interacts with cullins and CSN6 with Rbx1 [16,17] ."
Csn binds CSN2, COPS6, CUL, and RBX1. 1 / 1
| 1

sparser
"CSN physically interacts with the cullin and RBX1 subunits of CRLs through its subunits CSN2 and CSN6 and it is thought that these interactions provide CSN with the affinity for its CRL targets ( xref )."
COPS6 affects BTRC
| 1 4
COPS6 inhibits BTRC. 5 / 5
| 1 4

reach
"To test whether CSN6 increased the ubiquitinated degradation of β-TrCP in PASMCs stimulated with PDGF, cells were pre-treated with proteasome inhibitor MG-132 (10 μM) for 12 h, and then stimulated wit[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Furthermore, CSN6 has been found to decrease β-TrCP by promoting its autocatalytic degradation, thereby stabilizing SCF ubiquitin ligase substrate in colorectal cancer cells [22] ."

reach
"However, it is unclear whether up-regulation of CSN6 decreases β-TrCP by increasing its ubiquitinated degradation, thereby increasing the lifetime of Cdc25A in PDGF-stimulated PASMCs."

eidos
"This indicated that up-regulation of CSN6 by PDGFR / PI3K / Akt signaling promotes beta-TrCP degradation , which , in turn , stabilizes the cell cycle protein Cdc25A and results in PASMC proliferation ."

reach
"Epidermal-growth-factor (EGF)-induced extracellular signal-regulated kinase 2 (ERK2) activation phosphorylates CSN6 at S148, leading to β-catenin stabilization through blocking β-TrCP and colorectal cancer development [123]."
COPS6 affects Aldh1a1 mRNA
| 5
COPS6 activates Aldh1a1 mRNA. 5 / 5
| 5

eidos
"Importantly , CSN6 caused an increase in the Aldh1a1 mRNA level and that overexpression of TRIM21 led to a decrease in the Aldh1a1 mRNA level , even in the presence of CSN6 ( Fig. 2l ) ."

eidos
"In addition , quantitative RT-PCR analysis indicated that CSN6 knockdown caused a decrease in the Aldh1a1 mRNA level but failed to do so when TRIM21 was also knocked down ( Fig. 2m ) ."

eidos
"We further showed that CSN6 expression caused a significant increase in the Aldh1a1 mRNA level in control cells but not in TRIM21 KO cells ( Fig. 2n ) ."

eidos
"Accordingly , CSN6 knockdown led to a decrease in the level of Aldh1a1 mRNA ( Fig. 1d ) , while ectopic expression of CSN6 in both the HCT116 and DLD-1 cell lines resulted in increased Aldh1a1 mRNA levels ( Fig. 1d ) ."

eidos
"CSN6 regulates ALDH1A1 expression through TRIM21 To investigate how knockdown of CSN6 can lead to reduced expression of Aldh1a1 mRNA , we determined whether CSN6-associated proteins might have a role ."
4 |
Pirinixic acid increases the amount of COPS6. 4 / 4
4 |

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Fbxw1 affects COPS6
| 2 2
| 2 2

reach
"We found that CSN6 also associates with beta-Trcp and facilitates its degradation."

sparser
"We found that CSN6 also associates with β-Trcp and facilitates its degradation."

sparser
"We found that CSN6 associated with β-Trcp in vivo ( xref ), suggesting that CSN6 interacts with F-box protein β-Trcp and regulates it, thereby stabilizing β-catenin."

reach
"We found that CSN6 associated with beta-Trcp in vivo (XREF_FIG), suggesting that CSN6 interacts with F-box protein beta-Trcp and regulates it, thereby stabilizing beta-catenin."
UBE2M affects COPS6
4 |
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
TESPA1 affects COPS6
| 4
| 4

sparser
"Tespa1 interacts with CSN6 to inhibit c-Myc degradation in HSCs."

sparser
"Given that CSN6 is a potential Tespa1 interacting factor by protein-protein interaction analysis using STRING database (Supplementary Fig.  xref ), we first conducted an in situ proximity ligation assay and found that Tespa1 could interact with CSN6 in HSCs upon 5-FU and IR treatment (Supplementary Fig.  xref )."

sparser
"These data illustrate that Tespa1 can interact with CSN6 to inhibit c-Myc degradation in HSCs during hematopoietic stresses."

sparser
"Accordingly, it is reasonable to propose that Tespa1 can interact with CSN6 to inhibit c-Myc degradation in HSCs."
GST affects COPS6
| 4
| 4

sparser
"We then mixed the same amount of each of the expressed proteins with purified GST or GST-CSN6, followed by a GST pull-down assay ( Fig. 2 B)."

sparser
"For GST pull-down, purified GST, full-length or truncated CSN6-GST fusion proteins were mixed with His-tagged IN proteins individually, and then incubated with Glutathione-Sepharose beads for 0.5 h."

sparser
"We then examined the effect of increasing concentrations of the purified recombinant GST-CSN6 on IN activity with GST protein as the control and found that GST-CSN6 inhibited IN activity in a dose-dep[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Purified GST-CSN6 was relatively stable as shown in Coomassie blue staining ( Fig. 2 C)."
FOXA1 affects COPS6
| 1 3
| 1 3

sparser
"To assess the clinical relevance of the CSN6-FOXA1 regulatory axis in human PAAD progression, we analyzed the association between the expression levels of CSN6 and FOXA1 in 107 PAAD patients."

sparser
"Thus, we firstly observed whether there was interaction between CSN6 and FOXA1."

sparser
"As showed in Fig. S6 a, co-immunoprecipitation(co-IP) result suggested that there was no interaction between CSN6 protein and FOXA1 protein( Fig.S8 a )."

reach
"Thus, we firstly observed whether there was interaction between CSN6 and FOXA1."
FBXO7 affects COPS6
4 |
4 |

No evidence text available

No evidence text available

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No evidence text available
ELOB affects COPS6
3 | 1
3 | 1

reach
"Additionally, correlation and enrichment analyses identified two genes, GPS1 and TCEB2, associated with COPS6, which could be further explored to understand its mechanisms."

No evidence text available

No evidence text available

No evidence text available
EIF3E affects COPS6
2 1 |
2 1 |

No evidence text available

No evidence text available

No evidence text available
EGFP affects COPS6
| 4
| 4

sparser
"In our bleaching experiments, we used the localization of mCherry-tagged MKLP1 as a fiduciary for the position of the midbody and employed a high intensity 488 nm laser to simultaneously bleach mCherry-MKLP1 and EGFP-CSN6 (or EGFP) at the midbody."

sparser
"By plotting the mean intensity of the fluorescent signal for EGFP and EGFP-CSN6, and curve fitted using a non-linear regression and the exponential one-phase association model, it was found that CSN6 has a larger immobile fraction than EGFP ( xref )); a result consistent with a strong association with substructures within the midbody such as the contractile ring."

sparser
"Fluorescence recovery of EGFP was very rapid, while EGFP-CSN6 and mCherry-MKLP1 only recovered partially over the 5-min time period observed."

sparser
"Therefore, to further characterize the association of the CSN with the midbody, we examined the localization and diffusion of EGFP-CSN6 as a marker of the COP9 signalosome in HeLa cells by fluorescence recovery after photobleaching (FRAP) ( xref )."
DTL affects COPS6
4 |
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
CSN3 affects COPS6
| 4
CSN3 binds COPS5 and COPS6. 3 / 3
| 3

sparser
"Our data also demonstrate that CSNAP binds to CSN3, CSN5 and CSN6, possibly linking the MPN and PCI substructures."

sparser
"We show that CSNAP binds CSN3, CSN5 and CSN6 and its incorporation into the CSN complex is mediated through the C-terminal region involving conserved aromatic residues."

sparser
"CSNAP interacts with CSN3, CSN5, and CSN6."
COPS6 binds CSN3. 1 / 1
| 1

sparser
"In addition to the Csn5-Csn6 interaction, nine other interaction pairs were detected in this study, including Csn1-Csn2, Csn1-Csn3, Csn1-Csn4, Csn2-Csn3, Csn3-Csn4, Csn3-Csn6, Csn4-Csn6, Csn4-Csn7, and Csn6-Csn7 ( xref )."
COPS9 affects COPS6
4 |
4 |

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reach
"In this regard, Zou et al. reported that CSN6 mediates nucleotide metabolism to promote tumor development and chemoresistance in colorectal cancer (CRC) [85]."

reach
"A colitis-associated colorectal cancer model established that Csn6 intestinal conditional deletion decreased tumor development and altered nucleotide metabolism."

reach
"CSN6 mediates nucleotide metabolism to promote tumor development and chemoresistance in colorectal cancer."

eidos
"CSN6 mediates nucleotide metabolism to promote tumor development and chemoresistance in colorectal cancer ."
COPS6 affects UBE2M
4 |
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
COPS6 affects TESPA1
| 4
| 4

sparser
"Tespa1 interacts with CSN6 to inhibit c-Myc degradation in HSCs."

sparser
"Given that CSN6 is a potential Tespa1 interacting factor by protein-protein interaction analysis using STRING database (Supplementary Fig.  xref ), we first conducted an in situ proximity ligation assay and found that Tespa1 could interact with CSN6 in HSCs upon 5-FU and IR treatment (Supplementary Fig.  xref )."

sparser
"These data illustrate that Tespa1 can interact with CSN6 to inhibit c-Myc degradation in HSCs during hematopoietic stresses."

sparser
"Accordingly, it is reasonable to propose that Tespa1 can interact with CSN6 to inhibit c-Myc degradation in HSCs."
COPS6 affects SLC2A1
1 1 | 2
COPS6 increases the amount of SLC2A1.
| 2
COPS6 increases the amount of SLC2A1. 2 / 2
| 2

reach
"In contrast, protein analysis demonstrated that CSN6 knockdown reduced the expression of Glut1 while increased the expression of FOXO4 (Figure 5F)."

reach
"As CSN6 mitigates the expression level of FOXO4, we showed that CSN6 overexpression leads to increased gene expression of Glut1 (Figure 5F, Figure S15A, Supporting Information)."
COPS6 binds SLC2A1.
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects GST
| 4
| 4

sparser
"We then mixed the same amount of each of the expressed proteins with purified GST or GST-CSN6, followed by a GST pull-down assay ( Fig. 2 B)."

sparser
"For GST pull-down, purified GST, full-length or truncated CSN6-GST fusion proteins were mixed with His-tagged IN proteins individually, and then incubated with Glutathione-Sepharose beads for 0.5 h."

sparser
"We then examined the effect of increasing concentrations of the purified recombinant GST-CSN6 on IN activity with GST protein as the control and found that GST-CSN6 inhibited IN activity in a dose-dep[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Purified GST-CSN6 was relatively stable as shown in Coomassie blue staining ( Fig. 2 C)."
COPS6 affects FBXO7
4 |
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
COPS6 affects ERK
| 1 3
| 1 3

sparser
"In the present study, replacing some conserved residues on the D- site compromised CSN6’s capacity to interact with ERK, suggesting that these residues are involved in ERK-CSN6 protein-protein interaction."

reach
"A coimmunoprecipitation assay revealed that ERK1/2 binds to CSN6 (XREF_FIG)."

sparser
"A coimmunoprecipitation assay revealed that ERK1/2 binds to CSN6 ( xref )."

sparser
"Model of CSN6-ERK Interaction."
COPS6 affects ELOB
3 | 1
3 | 1

reach
"Additionally, correlation and enrichment analyses identified two genes, GPS1 and TCEB2, associated with COPS6, which could be further explored to understand its mechanisms."

No evidence text available

No evidence text available

No evidence text available
COPS6 affects EIF3E
2 1 |
2 1 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects EGFP
| 4
| 4

sparser
"In our bleaching experiments, we used the localization of mCherry-tagged MKLP1 as a fiduciary for the position of the midbody and employed a high intensity 488 nm laser to simultaneously bleach mCherry-MKLP1 and EGFP-CSN6 (or EGFP) at the midbody."

sparser
"By plotting the mean intensity of the fluorescent signal for EGFP and EGFP-CSN6, and curve fitted using a non-linear regression and the exponential one-phase association model, it was found that CSN6 has a larger immobile fraction than EGFP ( xref )); a result consistent with a strong association with substructures within the midbody such as the contractile ring."

sparser
"Fluorescence recovery of EGFP was very rapid, while EGFP-CSN6 and mCherry-MKLP1 only recovered partially over the 5-min time period observed."

sparser
"Therefore, to further characterize the association of the CSN with the midbody, we examined the localization and diffusion of EGFP-CSN6 as a marker of the COP9 signalosome in HeLa cells by fluorescence recovery after photobleaching (FRAP) ( xref )."
COPS6 affects DTL
4 |
4 |

No evidence text available

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No evidence text available

No evidence text available
COPS6 affects CSN3
| 4
CSN3 binds COPS5 and COPS6. 3 / 3
| 3

sparser
"Our data also demonstrate that CSNAP binds to CSN3, CSN5 and CSN6, possibly linking the MPN and PCI substructures."

sparser
"We show that CSNAP binds CSN3, CSN5 and CSN6 and its incorporation into the CSN complex is mediated through the C-terminal region involving conserved aromatic residues."

sparser
"CSNAP interacts with CSN3, CSN5, and CSN6."
COPS6 binds CSN3. 1 / 1
| 1

sparser
"In addition to the Csn5-Csn6 interaction, nine other interaction pairs were detected in this study, including Csn1-Csn2, Csn1-Csn3, Csn1-Csn4, Csn2-Csn3, Csn3-Csn4, Csn3-Csn6, Csn4-Csn6, Csn4-Csn7, and Csn6-Csn7 ( xref )."
COPS6 affects COPS9
4 |
4 |

No evidence text available

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TOR1AIP2 affects COPS6
3 |

No evidence text available

No evidence text available

No evidence text available
TK1 affects COPS6
2 1 |
2 1 |

No evidence text available

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Snail1 affects COPS6
| 3
COPS6 binds Snail1. 3 / 3
| 3

reach
"Co-immunoprecipitation study was used to show the interaction between the protein CSN6 and Snail1."

reach
"We next investigated how CSN6 interacted with Snail1 to regulate the protein level of Snail1."

reach
"Western Blot analysis showed that CSN6 could up-regulate the expression of Snail1 protein in a dose dependent manner, and CO-IP analysis showed that CSN6 could bind with Snail1."
STUB1 affects COPS6
| 1 1 1
| 1 1 1

trips
"We showed that CSN6 associated with CHIP and led to CHIP destabilization by increasing CHIP self-ubiquitination."

reach
"We showed that CSN6 associated with CHIP and led to CHIP destabilization by increasing CHIP self ubiquitination."

sparser
"We showed that CSN6 associated with CHIP and led to CHIP destabilization by increasing CHIP self-ubiquitination."
RPL15 affects COPS6
2 1 |
2 1 |

No evidence text available

No evidence text available

No evidence text available
RPA2 affects COPS6
2 1 |
2 1 |

No evidence text available

No evidence text available

No evidence text available
PRSS27 affects COPS6
| 3
| 3

sparser
"CSN5 alone is inactive due to an auto-inhibited conformation of its catalytic domain, and the association of CSN5 with the CSN6 MPN (Mpr1/Pad1 N-terminal) domain activates its isopeptidase activity xref ."

sparser
"The association of CSN5 and CSN6 MPN (for Mpr1/Pad1 N-terminal) domains activates its isopeptidase activity."

sparser
"Here we report that CSN6 N-terminal domain containing its MPN domain can form a stable heterodimer with CSN5 catalytic domain in vitro ."
PI3K affects COPS6
| 3
PI3K increases the amount of COPS6. 3 / 3
| 3

reach
"However, it remains uncertain whether suppression of β-TrCP by up-regulation of CSN6 facilitates Cdc25A ubiquitinated degradation and subsequently promotes cell proliferation.Recent studies have repor[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Therefore, it is important to investigate whether activation of PI3K/Akt signaling pathway increases CSN6 expression in PASMCs stimulated with PDGF."

reach
"Therefore, activation of PI3K/Akt signaling pathway increases the expression of CSN6 in cancer cells."
PDGFR affects COPS6
| 1 2
PDGFR activates COPS6. 3 / 3
| 1 2

reach
"These results suggest that PDGFR mediates PDGF-induced up-regulation of CSN6 in PASMCs.Next, we examined whether activation of PDGFR mediates down-regulation of β-TrCP in PDGF-stimulated PASMCs."

eidos
"This indicated that up-regulation of CSN6 by PDGFR / PI3K / Akt signaling promotes beta-TrCP degradation , which , in turn , stabilizes the cell cycle protein Cdc25A and results in PASMC proliferation ."

reach
"These results indicate that PDGFR mediate PI3K/Akt signaling pathway activation in PASMCs treated with PDGF.To examine whether activation of PDGFR mediates PDGF-induced up-regulation of CSN6 in PASMCs[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
NR3C1 affects COPS6
1 1 | 1
1 1 | 1

No evidence text available

No evidence text available

reach
"Human Vpr interacting protein (hVIP and Mov34), which binds to both Vpr and GR, translocates to the nucleus following either dexamethasone or Vpr treatment, further suggesting that Vpr and GR form an functional complex within cells [XREF_BIBR]."
LRRC14 affects COPS6
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
KLHL18 affects COPS6
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
KLHL15 affects COPS6
3 |
3 |

No evidence text available

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No evidence text available
KLHDC3 affects COPS6
3 |
3 |

No evidence text available

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No evidence text available
FBXW7 affects COPS6
| 2 1
| 2 1

reach
"The binding of COP9 signalosome subunit 6 (CSN6) to FBXW7β inhibits the degradation of fatty acid synthase (FASN), promoting lipogenic CRC and tumor progression (Wei et al., 2023)."

sparser
"CSN6’s association with Fbxw7 facilitates its degradation."

reach
"First, we found that CSN6 associated with Myc, Fbxw7 and Cullin-1 in U2OS cells in vivo (XREF_FIG), suggesting that CSN6 interacts with SCF complex and may regulate it to stabilize Myc."
FBXL6 affects COPS6
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
FBXL14 affects COPS6
3 |
3 |

No evidence text available

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ERCC6 affects COPS6
3 |
3 |

No evidence text available

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EP300 affects COPS6
2 1 |
2 1 |

No evidence text available

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No evidence text available
ELOC affects COPS6
3 |
3 |

No evidence text available

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No evidence text available
| 3

sparser
"This shuttling of p27 Kip1 from the nucleus to the cytoplasm could be mediated by additional interaction with CSN6 and the E3 ubiquitin ligase constitutive photomorphogenic 1 (COP1) [ xref ]."

sparser
"This study demonstrated that UBR5, a novel E3 ubiquitin ligase interacting with CSN6, was negatively regulated by CSN6 and was responsible for CDK9 ubiquitination and degradation in melanoma cells."

sparser
"COPS6 interacts with SCF-type E3 ubiquitin ligases and acts as a positive regulator of E3 ubiquitin ligases involved in various cellular and developmental processes [ 48 , 49 ]."
CTSL affects COPS6
| 1 2
| 1 2

reach
"Co-immunoprecipitation experiments showed endogenous interaction of CSN6 and CTSL in HeLa cells (Fig. 8A and B)."

sparser
"CSN6 interacts with CTSL and positively regulates CTSL expression through an autophagy-lysosomal system."

sparser
"Co-immunoprecipitation experiments showed endogenous interaction of CSN6 and CTSL in HeLa cells (Fig. xref A and B)."
CSN3 affects COPS5
| 3
CSN3 binds COPS5 and COPS6. 3 / 3
| 3

sparser
"Our data also demonstrate that CSNAP binds to CSN3, CSN5 and CSN6, possibly linking the MPN and PCI substructures."

sparser
"We show that CSNAP binds CSN3, CSN5 and CSN6 and its incorporation into the CSN complex is mediated through the C-terminal region involving conserved aromatic residues."

sparser
"CSNAP interacts with CSN3, CSN5, and CSN6."
CRBN affects COPS6
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects quercetin
| 3
| 3

reach
"The overexpression of CSN6 reduced the effect of quercetin treatment on HT-29 cells, suggesting that quercetin induced apoptosis may involve the Akt-CSN6-Myc signaling axis in HT-29 cells."

reach
"The MTT assay revealed that the overexpression of CSN6 reduced the effect of quercetin on cell viability compared with the empty plasmid MIGR1 (XREF_FIG)."

reach
"The MTT assay revealed that the overexpression of CSN6 reduced the effect of quercetin on cell viability compared with the empty MIGR1 plasmid (XREF_FIG)."
COPS6 affects migration
| 3
COPS6 activates migration. 3 / 3
| 3

eidos
"CSN6 promotes GBM proliferation , migration , invasion , and tumorigenesis through upregulation of EGFR by blocking its ubiquitination ; this happens as a result of interactions with CHIP that cause its degradation , although CHIP auto-ubiquitination occurs through an unknown mechanism [ 91 ] ."

eidos
"In melanoma cells , CSN6 knockdown remarkably inhibited cell proliferation , tumorigenicity , migration , and invasion , whereas CSN6 recovery rescued the proliferative and metastatic abilities ."

eidos
"In summary , these findings demonstrated that downregulation of CSN6 expression could inhibit cell proliferation , migration and invasion ."

reach
"COP9 signalosome subunit 6 (CSN6), an oncogenic marker of CRC, increases lipogenesis via interacting with and stabilizing FASN."

reach
"Together, these results demonstrated that CSN6 and FBXW7β participated in the vicissitude of FASN’s stability.Next we performed a phenotypic rescue experiment, asking whether CSN6-FBXW7β relationship is involved in CSN6-mediated lipogenesis and cell growth in CRC cells."

reach
"CSN6-induced lipogenesis facilitates CRC tumor growth, which can be suppressed by FASN inhibitor orlistat."

reach
"Bioinformatics analysis suggested that COPS6 was a mediator of IL-6 production in the tumor microenvironment and a negative regulator of CD8 + T cell tumor infiltration in breast cancer."

reach
"COPS6 promotes tumor progression and reduces CD8+ T cell infiltration by repressing IL-6 production to facilitate tumor immune evasion in breast cancer."

reach
"It has been observed that COPS6 is a mediator of IL-6 production in the tumor microenvironment and a suppressor of CD8+ T cell tumor infiltration[16]."
| 3

reach
"Through combinatorial effects on the MDM2-p53 signaling axis and now SKP2 mediated p57 Kip2 degradation, it is hypothesized that CSN6 overexpression can effectively promote cell proliferation by simultaneously relieving cell cycle restraints, apoptosis, senescence, and presumably p53 functions in DNA damage repair and cell metabolism, among other important tumor suppressive mechanisms."

reach
"CSN6 knockdown increased apoptosis as evident in increased Annexin V staining when compared with vector control."

reach
"CSN6 knockdown also accelerated apoptosis as evident in increased sub-G1 population after propidium iodide staining followed by flow cytometry."
COPS6 affects TOR1AIP2
3 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects TK1
2 1 |
2 1 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects Snail1 protein
| 1 2
COPS6 activates Snail1 protein.
| 1 1
COPS6 activates Snail1 protein. 2 / 2
| 1 1

reach
"The above results prompted us to study how CSN6 up-regulates Snail1 protein."

eidos
"The above results prompted us to study how CSN6 up-regulates Snail1 protein ."
COPS6 increases the amount of Snail1 protein.
| 1
COPS6 increases the amount of Snail1 protein. 1 / 1
| 1

reach
"Western Blot analysis showed that CSN6 could up-regulate the expression of Snail1 protein in a dose dependent manner, and CO-IP analysis showed that CSN6 could bind with Snail1."
COPS6 affects RPL15
2 1 |
2 1 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects RPA2
2 1 |
2 1 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects PRSS27
| 3
| 3

sparser
"CSN5 alone is inactive due to an auto-inhibited conformation of its catalytic domain, and the association of CSN5 with the CSN6 MPN (Mpr1/Pad1 N-terminal) domain activates its isopeptidase activity xref ."

sparser
"The association of CSN5 and CSN6 MPN (for Mpr1/Pad1 N-terminal) domains activates its isopeptidase activity."

sparser
"Here we report that CSN6 N-terminal domain containing its MPN domain can form a stable heterodimer with CSN5 catalytic domain in vitro ."
COPS6 affects ORF1
| 3
COPS6 activates ORF1.
| 2
COPS6 activates ORF1. 2 / 2
| 2

reach
"CSN6 associates with p57 (Kip2), and its overexpression can decrease the steady-state expression of p57 (Kip2); accordingly, CSN6 deficiency leads to p57 (Kip2) stabilization."

reach
"CDK inhibitor p57 (Kip2) is negatively regulated by COP9 signalosome subunit 6."
COPS6 inhibits ORF1.
| 1
COPS6 inhibits ORF1. 1 / 1
| 1

reach
"Loss of Skp2 compromised CSN6 mediated p57 (Kip2) destabilization, suggesting collaboration between Skp2 and CSN6 in degradation of p57 (Kip2)."
COPS6 affects NR3C1
1 1 | 1
1 1 | 1

No evidence text available

No evidence text available

reach
"Human Vpr interacting protein (hVIP and Mov34), which binds to both Vpr and GR, translocates to the nucleus following either dexamethasone or Vpr treatment, further suggesting that Vpr and GR form an functional complex within cells [XREF_BIBR]."
COPS6 affects LRRC14
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects KLHL18
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects KLHL15
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects KLHDC3
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects Hypertrophy
| 3
| 3

reach
"What's more, we knocked down Nkx2.2 in CSN6-overexpressing NRCMs, and found that the downregulation of Nkx2.2 rescued the decrease of SIRT2's expression and cardiomyocyte hypertrophy caused by overexp[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Down-regulation of CSN6 could attenuate Ang II-induced cardiomyocyte hypertrophy based on the results that the size of cardiomyocyte and the expression levels of fetal genes were decreased in CSN6-sil[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Further, we upregulated Nkx2.2 in CSN6-silencing NRCMs, and found that overexpression of Nkx2.2 attenuated the increase of SIRT2's expression and cardiomyocyte hypertrophy induced by CSN6 inhibition ([MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
COPS6 affects FBXL6
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects FBXL14
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects ERCC6
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects EP300
2 1 |
2 1 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects ELOC
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects CRBN
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
COPS6 affects CDC25A
| 3
COPS6 increases the amount of CDC25A. 3 / 3
| 3

reach
"Fig. 5 C shows that PDGF significantly increased Cdc25A protein level ( P < 0.05), whereas loss of CSN6 by siRNA silencing decreased the protein level of Cdc25A from 1.74-fold to 0.91-fold over contro[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Therefore, we speculate that CSN6 decreases β-TrCP and subsequently increases the expression of Cdc25A by competition with CSN5 and thereby inhibition of cullin deneddylation."

reach
"To further investigate whether elevation of CSN6 up-regulates the expression of Cdc25A by increasing β-TrCP ubiquitinated degradation in PDGF-stimulated PASMCs, cells were incubated with MG-132 (10 μM[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
COPS6 affects CD274
| 3
COPS6 inhibits CD274.
| 2
COPS6 inhibits CD274. 2 / 2
| 2

reach
"CSN6 and Jab1/CSN5 inhibit the degradation of PD-L1 and subsequently sustain PD-L1 stability in cancer cells ."

reach
"COPS5 and COPS6 can prevent PD-L1 from degrading, thereby maintaining PD-L1 stability in cancer cells [69, 70]."
COPS6 increases the amount of CD274.
| 1
COPS6 increases the amount of CD274. 1 / 1
| 1

reach
"Inhibition of CSN6 by small interfering RNA decreased PD-L1 expression but also increased CHIP expression in GBM cells."
COPS6 affects ALDH1A1
| 3
COPS6 increases the amount of ALDH1A1. 3 / 3
| 3

reach
"We found that the expression of CSN6 increased the steady-state protein expression levels of OCT1 and ALDH1A1, with a concurrent reduction in TRIM21 expression in a dose dependent manner."

reach
"Importantly, CSN6 caused an increase in the Aldh1a1 mRNA level and that overexpression of TRIM21 led to a decrease in the Aldh1a1 mRNA level, even in the presence of CSN6."

reach
"40 Interestingly, CSN6 is able to promote the expression of ALDH1A1, a known CSC marker upregulated in cancer spheroids."
COPS6 affects A0A288CFT1
3 |

No evidence text available

No evidence text available

No evidence text available
COPS5 affects PRSS27
| 3
| 3

sparser
"CSN5 alone is inactive due to an auto-inhibited conformation of its catalytic domain, and the association of CSN5 with the CSN6 MPN (Mpr1/Pad1 N-terminal) domain activates its isopeptidase activity xref ."

sparser
"The association of CSN5 and CSN6 MPN (for Mpr1/Pad1 N-terminal) domains activates its isopeptidase activity."

sparser
"Here we report that CSN6 N-terminal domain containing its MPN domain can form a stable heterodimer with CSN5 catalytic domain in vitro ."
COPS5 affects CSN3
| 3
CSN3 binds COPS5 and COPS6. 3 / 3
| 3

sparser
"Our data also demonstrate that CSNAP binds to CSN3, CSN5 and CSN6, possibly linking the MPN and PCI substructures."

sparser
"We show that CSNAP binds CSN3, CSN5 and CSN6 and its incorporation into the CSN complex is mediated through the C-terminal region involving conserved aromatic residues."

sparser
"CSNAP interacts with CSN3, CSN5, and CSN6."
COPS5 affects COPS6, and PRSS27
| 3
| 3

sparser
"CSN5 alone is inactive due to an auto-inhibited conformation of its catalytic domain, and the association of CSN5 with the CSN6 MPN (Mpr1/Pad1 N-terminal) domain activates its isopeptidase activity xref ."

sparser
"The association of CSN5 and CSN6 MPN (for Mpr1/Pad1 N-terminal) domains activates its isopeptidase activity."

sparser
"Here we report that CSN6 N-terminal domain containing its MPN domain can form a stable heterodimer with CSN5 catalytic domain in vitro ."
COPS5 affects COPS6, and CSN3
| 3
CSN3 binds COPS5 and COPS6. 3 / 3
| 3

sparser
"Our data also demonstrate that CSNAP binds to CSN3, CSN5 and CSN6, possibly linking the MPN and PCI substructures."

sparser
"We show that CSNAP binds CSN3, CSN5 and CSN6 and its incorporation into the CSN complex is mediated through the C-terminal region involving conserved aromatic residues."

sparser
"CSNAP interacts with CSN3, CSN5, and CSN6."
COPS2 affects COPS3, COPS4, COPS5, COPS6, COPS7A, COPS8, and GPS1
1 2 |

"Inferred from SIGNOR complex SIGNOR-C489"

No evidence text available

No evidence text available
| 3
| 3

reach
"However, the specific DUB and the molecular mechanism that play roles in Ang-II induced pathological myocardial hypertrophy is still unclear.Deubiquitinase CSN6 belongs to JAMM/MPN domain-associated m[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"In the current study, we reported that Ang II induced the upregulation of CSN6, a deubiquitinase belongs to JAMMs class, in cardiomyocytes both in vivo and in vitro ."

reach
"Overall, our results indicated that CSN6 promoted Ang II-induced cardiac hypertrophy.Next, to determine molecular event of CSN6 regulating Ang II-induced myocardial hypertrophy, we applied high-throug[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
A0A288CFT1 affects COPS6
3 |

No evidence text available

No evidence text available

No evidence text available
P57 affects COPS6
| 2
COPS6 binds p57. 2 / 2
| 2

sparser
"CSN6 associates with p57 (Kip2) , and its overexpression can decrease the steady-state expression of p57 (Kip2) ; accordingly, CSN6 deficiency leads to p57 (Kip2) stabilization."

sparser
"Significantly, univariate Kaplan-Meier analysis of tumor samples demonstrates that high CSN6 expression or low p57 expression is associated with poor overall survival."
2 |
Cobalt dichloride decreases the amount of COPS6. 2 / 2
2 |

No evidence text available

No evidence text available

sparser
"For example, CSN6 can interact with amino-terminal caspase recruitment domain (CARD) of Nod1, a cytoplasmic protein that belongs to the Nod/NLR/CATERPILLER protein family and whose activation is involved in apoptotic pathways."

reach
"For example, CSN6 can interact with amino-terminal caspase recruitment domain (CARD) of Nod1, a cytoplasmic protein that belongs to the Nod/NLR/CATERPILLER protein family and whose activation is involved in apoptotic pathways."
Bisphenol A affects COPS6
2 |
Bisphenol A increases the amount of COPS6. 2 / 2
2 |

No evidence text available

No evidence text available
ZNF24 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
ZEB2 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
WIPI2 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
UBR5 affects COPS6
| 1 1
COPS6 binds UBR5 and A375. 1 / 1
| 1

reach
"The E3 ubiquitin ligase UBR5 has shown the ability to ubiquitinate CDK9 38, so we hypothesized that CSN6 stabilizes the expression of CDK9 by regulating the E3 ligase UBR5.A co-IP assay was performed and demonstrated that CSN6 interacted with UBR5 in A375 and MV3 melanoma cells."
| 1

sparser
"A co-IP assay was performed and demonstrated that CSN6 interacted with UBR5 in A375 and MV3 melanoma cells (Fig. xref )."
UBC affects COPS6
2 |
2 |

No evidence text available

No evidence text available
TRIB3 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
TRDMT1 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
TP53 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
Ser148 affects MAPK1
| 2
MAPK1 binds COPS6 and Ser148. 2 / 2
| 2

reach
"Mechanistic studies suggested that ERK2 directly binds to CSN6 Leu163/Val165 and phosphorylates COPS6 at Ser148, thereby regulating β-Trcp and stabilizing β-catenin expression, consequently blocking the ubiquitin-proteasome pathway and promoting CRC development.Programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) checkpoint blockade is an emerging immunotherapy modality in various tumors, while its regulatory mechanism remains uncertain."

reach
"Mechanistic studies revealed that CSN6 is deregulated by EGFR signaling, in which ERK2 binds directly to CSN6 Leu163 and Val165 and phosphorylates CSN6 at Ser148."
SYCE3 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
SULT1E1 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
STX5 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
STK40 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
STK11 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
SNRPG affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
SMN2 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
SLC2A1 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
SKP1 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
SERPINB9 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
SERPINA5 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
SAT1 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
S100A10 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
ROGDI affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
RFC5 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
RAE1 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
RAB40A affects COPS6
2 |
2 |

No evidence text available

No evidence text available
RAB27A affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
QTRT1 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
PTEN affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
PSMD11 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
PSAP affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
PRKRA affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
PMF1 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
PHYHIP affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
PFKL affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
PDZK1IP1 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
PAFAH1B3 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
PAEP affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
ORAI2 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
NOD1 affects COPS6
2 |
2 |

No evidence text available

No evidence text available

reach
"Further, CSN6‐mediated FOXO4 down regulation was rescued by the proteasome inhibitor MG132 (Figure 2D, Figure S5D, Supporting Information)."

reach
"In PAAD cells, shCSN6 could decrease the protein expression of c-Fos and overexpression of CSN6 could increase the protein expression of c-Fos (first, second and third lane),however,MG132, the proteas[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
MYCBP affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
MPN domain affects COPS6
| 2
COPS6 binds COPS5 and MPN domain. 2 / 2
| 2

reach
"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain, and the dimer is topologically knotted to engage in Cullin deneddylation."

reach
"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain and the dimer is topologically knotted to engage in Cullin deneddylation 55."
MNAT1 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
MDM2 affects COPS6
| 2
| 2

reach
"Similarly, overexpression of COPS6 binds to MDM2, and prevents the process of its auto-ubiquitination and degradation, which leads to the accumulation of MDM2 and decrease of p53 in breast cancer (49)."

reach
"CSN6 makes a contribution to the binding of CSN and E3 ligases [9,10] , and directly binds to MDM2, which is an ubiquitin ligases correlated with p53 pathway [11] ."
MAPK6 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
MAPK1 affects Ser148
| 2
MAPK1 binds COPS6 and Ser148. 2 / 2
| 2

reach
"Mechanistic studies suggested that ERK2 directly binds to CSN6 Leu163/Val165 and phosphorylates COPS6 at Ser148, thereby regulating β-Trcp and stabilizing β-catenin expression, consequently blocking the ubiquitin-proteasome pathway and promoting CRC development.Programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) checkpoint blockade is an emerging immunotherapy modality in various tumors, while its regulatory mechanism remains uncertain."

reach
"Mechanistic studies revealed that CSN6 is deregulated by EGFR signaling, in which ERK2 binds directly to CSN6 Leu163 and Val165 and phosphorylates CSN6 at Ser148."
MAPK1 affects Leu163
| 2
MAPK1 binds COPS6 and Leu163. 2 / 2
| 2

reach
"Mechanistic studies suggested that ERK2 directly binds to CSN6 Leu163/Val165 and phosphorylates COPS6 at Ser148, thereby regulating β-Trcp and stabilizing β-catenin expression, consequently blocking the ubiquitin-proteasome pathway and promoting CRC development.Programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) checkpoint blockade is an emerging immunotherapy modality in various tumors, while its regulatory mechanism remains uncertain."

reach
"Mechanistic studies revealed that CSN6 is deregulated by EGFR signaling, in which ERK2 binds directly to CSN6 Leu163 and Val165 and phosphorylates CSN6 at Ser148."
MAPK affects COPS6
| 1 1
| 1 1

sparser
"The model reveals that CSN6 D-site interacts with the MAPK docking groove."

reach
"To gain insight into the structural basis of CSN6 's recognition by MAPK, we used HADDOCK to generate a model of the CSN6 and MAPK complex."
MAP7D1 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
Leu163 affects MAPK1
| 2
MAPK1 binds COPS6 and Leu163. 2 / 2
| 2

reach
"Mechanistic studies suggested that ERK2 directly binds to CSN6 Leu163/Val165 and phosphorylates COPS6 at Ser148, thereby regulating β-Trcp and stabilizing β-catenin expression, consequently blocking the ubiquitin-proteasome pathway and promoting CRC development.Programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) checkpoint blockade is an emerging immunotherapy modality in various tumors, while its regulatory mechanism remains uncertain."

reach
"Mechanistic studies revealed that CSN6 is deregulated by EGFR signaling, in which ERK2 binds directly to CSN6 Leu163 and Val165 and phosphorylates CSN6 at Ser148."
LPL affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
LAMA4 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
KLHL9 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
KLHL8 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
KLHL42 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
KLHL22 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
KLHL13 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
KLHL10 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
KCTD18 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
HUWE1 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
HSPA5 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
HSPA1A affects COPS6
2 |
2 |

No evidence text available

No evidence text available
HMOX2 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
FBXW9 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
FBXW7β affects COPS6
| 2
COPS6 binds FBXW7β. 2 / 2
| 2

sparser
"Domain mapping studies indicated that CSN6 associates with FBXW7β through MPN domain containing N-Ter (Supplementary Fig. xref h, i)."

sparser
"The co-IP experiment showed the endogenous interaction between CSN6 and FBXW7β (Fig. xref )."
FBXO6 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
FBXO44 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
FBXO42 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
FBXO22 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
FBXO11 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
FBXL5 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
FAU affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
ERH affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
EMD affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
EDN1 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
Dtl affects COPS6
2 |
2 |

No evidence text available

No evidence text available
Ddb1 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
DLEU1 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
DIS3L2 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
DET1 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
DENR affects COPS6
2 |
2 |

No evidence text available

No evidence text available
DCAF16 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
CUL affects CSN2
| 2
CSN2 binds RING-box protein 1, CUL, and COPS6. 1 / 1
| 1

sparser
"The CSN becomes assembled in supercomplexes with CRLs in which CSN2 interacts with cullins and CSN6 with Rbx1 [16,17] ."
Csn binds CSN2, COPS6, CUL, and RBX1. 1 / 1
| 1

sparser
"CSN physically interacts with the cullin and RBX1 subunits of CRLs through its subunits CSN2 and CSN6 and it is thought that these interactions provide CSN with the affinity for its CRL targets ( xref )."
CSN1S1 affects COPS6
| 2
| 2

reach
"Interestingly, the complex is composed of two modules, Csn1/2/3/8 and Csn4/5/6/7, connected by interactions between Csn1 and Csn6."

reach
"Interestingly, we found that the COP9 and signalosome in C. elegans may be physically associated with the CUL-3 complex, as we were able to detect an interaction between CSN-1 and CSN-6 and the ring-finger protein ZK287.5."
CRIPTO affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
CRELD1 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
COX5A affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
COX17 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects v-myc avian myelocytomatosis viral oncogene homolog
| 2
COPS6 activates v-myc avian myelocytomatosis viral oncogene homolog. 2 / 2
| 2

reach
"A recent study determined that CSN6 promotes carcinogenesis by positively regulating v-myc avian myelocytomatosis viral oncogene homolog (Myc) stability."

reach
"CSN6 may promote carcinogenesis by positively regulating v-myc avian myelocytomatosis viral oncogene homolog (Myc) and MDM2 proto-oncogene stability, and is regarded as a potential target for cancer therapy."
COPS6 affects turnover OCT1
| 2
COPS6 inhibits turnover OCT1. 2 / 2
| 2

eidos
"c In DLD-1 cells expressing scrambled or CSN6-specific shRNA , CSN6 knockdown reduced the turnover rate of the TRIM21 protein and increased the turnover rate of OCT1 ."

eidos
"Indeed , CSN6 knockdown led to decelerated turnover of TRIM21 but accelerated turnover of OCT1 ( Fig. 4c ) ."
COPS6 affects steady-state TRIM21
| 2
COPS6 inhibits steady-state TRIM21. 2 / 2
| 2

eidos
"Tripartite motif ( TRIM ) protein family members ( numbering > 70 ) have been implicated in various cellular functions , including cell proliferation , differentiation , development , apoptosis , antiviral activity , autophagy and oncogenesis.44 TRIM21 plays a pivotal role in immune activation during pathogen infection , but its cellular function remains unclear.45 Interestingly , CSN6 is able to decrease the steady-state expression of TRIM21 by enhancing TRIM21 ubiquitination , thereby promoting cancer stemness ."

eidos
"In HCT116 cells , CSN6 reduced the steady-state level of TRIM21 , and MLN4924 treatment reversed this effect ( Fig. 3g ) ."
COPS6 affects pyrimidine
| 2
| 2

reach
"4 CSN6 promotes the development and chemoresistance of colorectal cancer through upregulating purine and pyrimidine biosynthesis."

reach
"Mechanistically, CSN6 upregulated purine and pyrimidine biosynthesis by increasing expression of PHGDH, a key enzyme in the serine synthesis pathway."
COPS6 affects purine
| 2
COPS6 activates purine. 2 / 2
| 2

reach
"Mechanistically, CSN6 upregulated purine and pyrimidine biosynthesis by increasing expression of PHGDH, a key enzyme in the serine synthesis pathway."

reach
"4 CSN6 promotes the development and chemoresistance of colorectal cancer through upregulating purine and pyrimidine biosynthesis."
COPS6 affects pathway
| 2
COPS6 activates pathway. 2 / 2
| 2

sparser
"Furthermore, we found that UBR5 knockdown rescued all the effects induced by CSN6 silencing, indicating that CSN6 activates the CDK9 pathway to promote melanoma growth and metastasis by reducing the UBR5 level."

sparser
"Furthermore, in CSN6-knockdown melanoma cells, UBR5 knockdown abrogated the effects caused by CSN6 silencing, suggesting that CSN6 activates the UBR5/CDK9 pathway to promote melanoma cell proliferation and metastasis."
COPS6 affects p57
| 2
COPS6 binds p57. 2 / 2
| 2

sparser
"CSN6 associates with p57 (Kip2) , and its overexpression can decrease the steady-state expression of p57 (Kip2) ; accordingly, CSN6 deficiency leads to p57 (Kip2) stabilization."

sparser
"Significantly, univariate Kaplan-Meier analysis of tumor samples demonstrates that high CSN6 expression or low p57 expression is associated with poor overall survival."

sparser
"For example, CSN6 can interact with amino-terminal caspase recruitment domain (CARD) of Nod1, a cytoplasmic protein that belongs to the Nod/NLR/CATERPILLER protein family and whose activation is involved in apoptotic pathways."

reach
"For example, CSN6 can interact with amino-terminal caspase recruitment domain (CARD) of Nod1, a cytoplasmic protein that belongs to the Nod/NLR/CATERPILLER protein family and whose activation is involved in apoptotic pathways."

reach
"4 CSN6 promotes the development and chemoresistance of colorectal cancer through upregulating purine and pyrimidine biosynthesis."

reach
"Mechanistically, CSN6 upregulated purine and pyrimidine biosynthesis by increasing expression of PHGDH, a key enzyme in the serine synthesis pathway."
COPS6 affects ZNF24
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects ZNF197
| 2
COPS6 increases the amount of ZNF197.
| 1
COPS6 increases the amount of ZNF197. 1 / 1
| 1

reach
"Further, COPS6 or COPS9 overexpression enhanced the expression levels of cell cycle regulators CyclinB1 and CDK4, but downregulated the expression of cyclin-dependent kinase inhibitors p18 and p21 according to western blot analysis (Figure 11D)."
COPS6 decreases the amount of ZNF197.
| 1
COPS6 decreases the amount of ZNF197. 1 / 1
| 1

reach
"Here, we suggested that COPS6 and COPS9 were able to downregulate the expression of p18 and p21."
COPS6 affects ZEB2
1 1 |
1 1 |

No evidence text available

No evidence text available
| 1 1

eidos
"HVIPs have been reported to reduce both trauma and criminal justice recidivism ( Cooper et al ., 2006 ; Shibru et al ., 2007 ) ; however , there is limited and mixed evidence to support their effectiveness ( Affinati et al ., 2016 ; Chong et al ., 2015 ; Cooper et al ., 2006 ; Dicker , 2016 ; Juillard et al ., 2016 ; Purtle et al ., 2013 ; Smith et al ., 2013 ) ."

reach
"Hospital-based violence intervention programs (HVIP) have been shown to decrease symptoms of trauma."
COPS6 affects WIPI2
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects UBC
2 |
2 |

No evidence text available

No evidence text available
COPS6 affects TRIB3
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects TRDMT1
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects TIMP2
| 2
COPS6 activates TIMP2. 2 / 2
| 2

reach
"Bioinformatics and Luciferase reporter genes demonstrated that CSN6 can target the corresponding sites of TIMP-2 promoter."

reach
"Additionally, we verified that CSN6 may promote OSCC malignant progression by regulating TIMP-2."
| 2

reach
"These results suggested that CSN6 may promote the cell proliferation and colony formation of GC cells."

reach
"Taken together, these data demonstrated that CSN6 could promote cell cycle progression in GC cells."
COPS6 affects Snail1 stability
| 2
COPS6 activates Snail1 stability. 2 / 2
| 2

eidos
"The above results suggest that CSN6 promotes Snail1 stability via impeding ubiquitination of Snail1 ."

eidos
"Taken together , these results indicated that CSN6 promotes Snail1 stability through reducing ubiquitination ."
COPS6 affects Ser148
| 2
MAPK1 binds COPS6 and Ser148. 2 / 2
| 2

reach
"Mechanistic studies suggested that ERK2 directly binds to CSN6 Leu163/Val165 and phosphorylates COPS6 at Ser148, thereby regulating β-Trcp and stabilizing β-catenin expression, consequently blocking the ubiquitin-proteasome pathway and promoting CRC development.Programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) checkpoint blockade is an emerging immunotherapy modality in various tumors, while its regulatory mechanism remains uncertain."

reach
"Mechanistic studies revealed that CSN6 is deregulated by EGFR signaling, in which ERK2 binds directly to CSN6 Leu163 and Val165 and phosphorylates CSN6 at Ser148."
COPS6 affects SYCE3
2 |
2 |

No evidence text available

No evidence text available
COPS6 affects SULT1E1
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects STX5
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects STK40
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects STK11
2 |
2 |

No evidence text available

No evidence text available
COPS6 affects SNRPG
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects SMN2
2 |
2 |

No evidence text available

No evidence text available
COPS6 affects SKP1
2 |
2 |

No evidence text available

No evidence text available
COPS6 affects SERPINB9
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects SERPINA5
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects SAT1
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects S100A10
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects S phase
| 2
COPS6 activates S phase. 2 / 2
| 2

reach
"Increasing expression of CSN6 significantly decreased the G1 cell population and increased the S phase cells, showing that overexpression of CSN6 markedly enhanced the G1/S transition."

reach
"Consistently, the attenuation of CSN6 expression significantly increased the G1 cell population and decreased the S phase cells, showing that the attenuation of CSN6 expression in GC cells markedly arrested the G1/S transition (Figure 2D bottom panel)."
COPS6 affects ROGDI
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects RFC5
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects RAE1
2 |
2 |

No evidence text available

No evidence text available
COPS6 affects RAB40A
2 |
2 |

No evidence text available

No evidence text available
COPS6 affects RAB27A
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects QTRT1
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects PTEN
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects PSMD11
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects PSAP
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects PRKRA
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects PMF1
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects PHYHIP
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects PFKL
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects PER16
| 2
COPS6 inhibits PER16. 2 / 2
| 2

reach
"Is there another manner of regulation for CSN6 to decrease p16 protein other than CSN6 controlling ubiquitination?"

reach
"The above results suggested that CSN6 downregulated p16 protein at the post-transcriptional level."
COPS6 affects PDZK1IP1
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects PDGF
| 2
COPS6 activates PDGF. 2 / 2
| 2

reach
"Our study provides important insights into the mechanisms underlying up-regulated CSN6 promotion of PDGF-stimulated PASMCs proliferation and suggests that CSN6 might be an efficient target to prevent [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Our study provides important insights into the mechanisms that up-regulated CSN6 mediates PDGF-induced PASMCs proliferation and suggests that CSN6 might be an efficient target for PAH prevention and t[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
COPS6 affects PAFAH1B3
1 1 |
1 1 |

No evidence text available

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COPS6 affects PAEP
1 1 |
1 1 |

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COPS6 affects ORAI2
1 1 |
1 1 |

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COPS6 affects NOD1
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COPS6 affects MYCBP
1 1 |
1 1 |

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COPS6 affects MPN domain
| 2
COPS6 binds COPS5 and MPN domain. 2 / 2
| 2

reach
"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain, and the dimer is topologically knotted to engage in Cullin deneddylation."

reach
"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain and the dimer is topologically knotted to engage in Cullin deneddylation 55."
COPS6 affects MNAT1
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects MAPK6
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COPS6 affects MAPK1, and Ser148
| 2
MAPK1 binds COPS6 and Ser148. 2 / 2
| 2

reach
"Mechanistic studies suggested that ERK2 directly binds to CSN6 Leu163/Val165 and phosphorylates COPS6 at Ser148, thereby regulating β-Trcp and stabilizing β-catenin expression, consequently blocking the ubiquitin-proteasome pathway and promoting CRC development.Programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) checkpoint blockade is an emerging immunotherapy modality in various tumors, while its regulatory mechanism remains uncertain."

reach
"Mechanistic studies revealed that CSN6 is deregulated by EGFR signaling, in which ERK2 binds directly to CSN6 Leu163 and Val165 and phosphorylates CSN6 at Ser148."
COPS6 affects MAPK
| 1 1
| 1 1

sparser
"The model reveals that CSN6 D-site interacts with the MAPK docking groove."

reach
"To gain insight into the structural basis of CSN6 's recognition by MAPK, we used HADDOCK to generate a model of the CSN6 and MAPK complex."
COPS6 affects MAP7D1
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects Leu163, and MAPK1
| 2
MAPK1 binds COPS6 and Leu163. 2 / 2
| 2

reach
"Mechanistic studies suggested that ERK2 directly binds to CSN6 Leu163/Val165 and phosphorylates COPS6 at Ser148, thereby regulating β-Trcp and stabilizing β-catenin expression, consequently blocking the ubiquitin-proteasome pathway and promoting CRC development.Programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) checkpoint blockade is an emerging immunotherapy modality in various tumors, while its regulatory mechanism remains uncertain."

reach
"Mechanistic studies revealed that CSN6 is deregulated by EGFR signaling, in which ERK2 binds directly to CSN6 Leu163 and Val165 and phosphorylates CSN6 at Ser148."
COPS6 affects LPL
1 1 |
1 1 |

No evidence text available

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COPS6 affects LC3-II
| 2
COPS6 inhibits LC3-II. 2 / 2
| 2

reach
"Western blot assay revealed that CSN6 knockdown resulted in the down-regulation of mTOR and the up-regulation of LC3-II (Fig. 5A)."

reach
"Knockdown of CSN6 induced appropriate autophagy pathways and significantly increased LC3-II accumulation in our study.In recent years, autophagy has been reported to be associated with CTSL 26, 31, 32."
COPS6 affects LAMA4
1 1 |
1 1 |

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COPS6 affects KLHL9
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COPS6 affects KLHL8
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COPS6 affects KLHL42
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COPS6 affects KLHL22
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COPS6 affects KLHL13
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COPS6 affects KLHL10
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COPS6 affects KITLG
| 2
COPS6 inhibits KITLG. 2 / 2
| 2

reach
"Furthermore, CSN6 has been found to decrease β-TrCP by promoting its autocatalytic degradation, thereby stabilizing SCF ubiquitin ligase substrate in colorectal cancer cells [22] ."

reach
"Study in colorectal cancer has found that up-regulation of CSN6 by EGF decreases SCF ubiquitin ligase substrate receptor β-transduction repeat-containing protein (β-TrCP) by promoting its autocatalyti[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
COPS6 affects KCTD18
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COPS6 affects HUWE1
2 |
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COPS6 affects HSPA5
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COPS6 affects HSPA1A
2 |
2 |

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COPS6 affects HMOX2
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects FBXW9
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No evidence text available

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COPS6 affects FBXW7β
| 2
COPS6 binds FBXW7β. 2 / 2
| 2

sparser
"Domain mapping studies indicated that CSN6 associates with FBXW7β through MPN domain containing N-Ter (Supplementary Fig. xref h, i)."

sparser
"The co-IP experiment showed the endogenous interaction between CSN6 and FBXW7β (Fig. xref )."
COPS6 affects FBXO6
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COPS6 affects FBXO44
2 |
2 |

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COPS6 affects FBXO42
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COPS6 affects FBXO22
2 |
2 |

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COPS6 affects FBXO11
2 |
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COPS6 affects FBXL5
2 |
2 |

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COPS6 affects FAU
1 1 |
1 1 |

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COPS6 affects ERH
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects EMD
1 1 |
1 1 |

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COPS6 affects EDN1
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects Dtl
2 |
2 |

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COPS6 affects Ddb1
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2 |

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reach
"In addition, the luciferase reporter gene assay showed that CSN6 inhibited the transcription activity of SIRT2 promoter ( Fig.S3 E)."

reach
"What's more, the luciferase reporter gene assay showed that CSN6 promoted the transcription activity of FOXA1 promoter( Fig.S8 d )."
COPS6 affects DLEU1
1 1 |
1 1 |

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No evidence text available
COPS6 affects DIS3L2
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects DET1
2 |
2 |

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COPS6 affects DENR
2 |
2 |

No evidence text available

No evidence text available
COPS6 affects DCAF16
2 |
2 |

No evidence text available

No evidence text available
COPS6 affects CSN1S1
| 2
| 2

reach
"Interestingly, the complex is composed of two modules, Csn1/2/3/8 and Csn4/5/6/7, connected by interactions between Csn1 and Csn6."

reach
"Interestingly, we found that the COP9 and signalosome in C. elegans may be physically associated with the CUL-3 complex, as we were able to detect an interaction between CSN-1 and CSN-6 and the ring-finger protein ZK287.5."
COPS6 affects CRIPTO
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects CRELD1
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects CRC
| 2
COPS6 activates CRC. 2 / 2
| 2

reach
"Together, our studies demonstrate that the role of EGF/CSN6 in increasing FASN accumulation and promoting CRC malignancy can be recapitulated in clinical samples."

reach
"Multiple lines of evidence indicate that CSN6 promotes the gene expression of stemness markeraldehyde dehydrogenase 1 A1 (ALDH1A1), indicating that CSN6 expression initiates cancer stemness in CRC."
COPS6 affects CRC PDOs
| 1 1
COPS6 activates CRC PDOs. 2 / 2
| 1 1

reach
"We found that decreasing CSN6 expression via knockdown reduced the number and growth of CRC PDOs, supporting the role of CSN6 in regulating stemness during tumorigenicity."

eidos
"Knockdown of CSN6 reduced the number and the size of CRC PDOs formed ( Fig. 1g ) ."
COPS6 affects COX5A
1 1 |
1 1 |

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No evidence text available
COPS6 affects COX17
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects CKS1B
2 |
2 |

No evidence text available

No evidence text available
COPS6 affects CDKN2C
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects CDH10
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects CD8
| 1 1
COPS6 inhibits CD8. 2 / 2
| 1 1

eidos
"COPS6 promotes tumor progression and reduces CD8 + T cell infiltration by repressing IL-6 production to facilitate tumor immune evasion in breast cancer ."

reach
"COPS6, a member of the JAMM family, has recently been verified to facilitate tumor advancement and decrease the infiltration of CD8 T-cells by suppressing the synthesis of IL-6, thus promoting tumor immune escape from cancer [44, 45]."
COPS6 affects CCDC106
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects C4orf17
1 1 |
1 1 |

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COPS6 affects C1orf174
1 1 |
1 1 |

No evidence text available

No evidence text available
COPS6 affects BTBD9
2 |
2 |

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COPS6 affects APBB3
2 |
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COPS6 affects AP1
| 2
COPS6 activates AP1. 2 / 2
| 2

reach
"On the contrary, overexpression of CSN6 increased c-Fos's protein expression and AP-1 transcriptional activity ( Fig. S9 a-c)."

reach
"In the reporter luciferase assay, the knockdown of CSN6 in CFPAC-1 cells decreased the transcriptional activity of AP-1( Fig. 5 c)."
COPS6 affects ALV-J
| 2
COPS6 inhibits ALV-J. 2 / 2
| 2

reach
"The inhibition of IN activity by CSN6 in the above-mentioned in vitro assay hints that CSN6 may limit ALV-J production."

reach
"Interestingly, contrary to the known IN interacting host proteins whose roles in the viral life cycle are to facilitate IN functions [3,15,16] , we found CSN6 inhibited ALV-J IN activity in vitro ( Fi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
COPS6 affects 14-3-3sigma
| 2
COPS6 inhibits 14-3-3sigma. 2 / 2
| 2

reach
"Since CSN6 downregulates and thus antagonizes the activity of 14-3-3sigma, we reasoned that CSN6 would have a role in cell proliferation and anchorage independent growth."

reach
"This result suggests that downregulation of 14-3-3sigma by CSN6 is not dependent on p53 expression."
| 2

reach
"The ability of hVIP and analogues to stimulate cAMP generation paralleled their potencies in displacing 125I-VIP binding."

reach
"(D-Phe2) VIP, VIP [10-28], VIP [16-28], and (p-Cl-D-Phe6, Leu17) VIP, a putative VIP receptor antagonist, affected neither basal cAMP levels nor hVIP induced cAMP accumulation."
COPS5 affects MPN domain
| 2
COPS6 binds COPS5 and MPN domain. 2 / 2
| 2

reach
"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain, and the dimer is topologically knotted to engage in Cullin deneddylation."

reach
"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain and the dimer is topologically knotted to engage in Cullin deneddylation 55."
CKS1B affects COPS6
2 |
2 |

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No evidence text available
CDKN2C affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
CDH10 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
CCDC106 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
C4orf17 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
C1orf174 affects COPS6
1 1 |
1 1 |

No evidence text available

No evidence text available
BTBD9 affects COPS6
2 |
2 |

No evidence text available

No evidence text available
APBB3 affects COPS6
2 |
2 |

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AKT1 affects COPS6
1 1 |
AKT1 phosphorylates COPS6 on S60. 2 / 2
1 1 |

No evidence text available

"Mechanistic studies show that akt causes csn6 phosphorylation at ser 60, which, in turn, reduces ubiquitin-mediated protein degradation of csn6."
2 |

No evidence text available

No evidence text available
17alpha-ethynylestradiol increases the amount of COPS6. 2 / 2
2 |

No evidence text available

No evidence text available
Thiram affects COPS6
1 |
Thiram decreases the amount of COPS6. 1 / 1
1 |

No evidence text available
Tert-Butylhydroperoxide decreases the amount of COPS6. 1 / 1
1 |

No evidence text available
HsdR affects COPS6
| 1
| 1

reach
"In vitro experiments revealed that hPACAP and hVIP also bind to hSR and activate downstream signaling, but only at high concentrations."
1 |
Hsa-miR-328-3p decreases the amount of COPS6. 1 / 1
1 |

No evidence text available
Csn affects RBX1
| 1
Csn binds CSN2, COPS6, CUL, and RBX1. 1 / 1
| 1

sparser
"CSN physically interacts with the cullin and RBX1 subunits of CRLs through its subunits CSN2 and CSN6 and it is thought that these interactions provide CSN with the affinity for its CRL targets ( xref )."
1 |
Benzo[a]pyrene increases the amount of COPS6. 1 / 1
1 |

No evidence text available
Aristolochic acid A decreases the amount of COPS6. 1 / 1
1 |

No evidence text available
| 1

reach
"Also, CSN6-expressing cells treated with orlistat can lead to increased apoptosis as demonstrated by apoptosis marker cleaved PARP1 and Annexin IV/PI staining (Fig. 6c and Supplementary Fig. 4a)."
ZYG11B affects COPS6
1 |
1 |

No evidence text available
ZMAT2 affects COPS6
1 |
1 |

No evidence text available
USP9X affects COPS6
1 |
1 |

No evidence text available
UBR5 affects A375
| 1
COPS6 binds UBR5 and A375. 1 / 1
| 1

reach
"The E3 ubiquitin ligase UBR5 has shown the ability to ubiquitinate CDK9 38, so we hypothesized that CSN6 stabilizes the expression of CDK9 by regulating the E3 ligase UBR5.A co-IP assay was performed and demonstrated that CSN6 interacted with UBR5 in A375 and MV3 melanoma cells."
UBE2H affects COPS6
1 |
1 |

No evidence text available
TXN affects COPS6
1 |
1 |

No evidence text available
TUBB affects COPS6
1 |
1 |

No evidence text available
TP63 affects COPS6
1 |
1 |

No evidence text available
TEAD4 affects COPS6
1 |
1 |

No evidence text available
Smoke affects COPS6
1 |
Smoke decreases the amount of COPS6. 1 / 1
1 |

No evidence text available
SYNCRIP affects COPS6
1 |
1 |

No evidence text available
SNRNP70 affects COPS6
1 |
1 |

No evidence text available
SMC3 affects COPS6
1 |
1 |

No evidence text available
SCG2 affects COPS6
1 |
1 |

No evidence text available
RPS4X affects COPS6
1 |
1 |

No evidence text available
RPS20 affects COPS6
1 |
1 |

No evidence text available
RPLP2 affects COPS6
1 |
1 |

No evidence text available
RPL11 affects COPS6
1 |
1 |

No evidence text available
RPA1 affects COPS6
1 |
1 |

No evidence text available
RING-box protein 1 affects CUL
| 1
CSN2 binds RING-box protein 1, CUL, and COPS6. 1 / 1
| 1

sparser
"The CSN becomes assembled in supercomplexes with CRLs in which CSN2 interacts with cullins and CSN6 with Rbx1 [16,17] ."
REG1A affects COPS6
| 1
REG1A inhibits COPS6. 1 / 1
| 1

reach
"Furthermore, the decrease of REGγ could rescue CSN6-mediated downregulation of p16."
RBX1 affects csn
| 1
Csn binds CSN2, COPS6, CUL, and RBX1. 1 / 1
| 1

sparser
"CSN physically interacts with the cullin and RBX1 subunits of CRLs through its subunits CSN2 and CSN6 and it is thought that these interactions provide CSN with the affinity for its CRL targets ( xref )."
RBM14 affects COPS6
1 |
1 |

No evidence text available
PWP1 affects COPS6
1 |
1 |

No evidence text available
PSMD14 affects COPS6
1 |
1 |

No evidence text available
POP7 affects COPS6
1 |
1 |

No evidence text available
PBX2 affects COPS6
1 |
1 |

No evidence text available
PABPC1 affects COPS6
1 |
1 |

No evidence text available
NHERF2 affects COPS6
1 |
1 |

No evidence text available
MFSD14A affects COPS6
1 |
1 |

No evidence text available
MBTD1 affects COPS6
1 |
1 |

No evidence text available
MAP2K1 affects COPS6
| 1
Modified MAP2K1 increases the amount of COPS6. 1 / 1
| 1

reach
"In addition, the expression of a constitutively active MEK1 (MEK1 CA) increased CSN6 expression in DLD-1 cells (XREF_SUPPLEMENTARY)."
M affects COPS6
1 |
COPS6 binds M. 1 / 1
1 |

No evidence text available
LYZ affects COPS6
1 |
1 |

No evidence text available
LOC100128400 affects COPS6
1 |
COPS6 binds LOC100128400. 1 / 1
1 |

No evidence text available
LARP4 affects COPS6
1 |
1 |

No evidence text available
KRT75 affects COPS6
1 |
1 |

No evidence text available
KRT24 affects COPS6
1 |
1 |

No evidence text available
KCTD9 affects COPS6
1 |
1 |

No evidence text available
IRF8 affects COPS6
1 |
1 |

No evidence text available
IGHV3OR15-7 affects COPS6
1 |

No evidence text available
HSPA12A affects COPS6
1 |
1 |

No evidence text available
HSP90B1 affects COPS6
1 |
1 |

No evidence text available
HSP90AB1 affects COPS6
1 |

No evidence text available
HMGCS1 affects COPS6
| 1
HMGCS1 activates COPS6. 1 / 1
| 1

reach
"Further, we collected tumor tissues and found that HMGCS1 overexpression rescued CSN6 KD‐mediated decreased cholesterol and TG levels, and YAP1 target gene expression (Figure 3G,H)."
HAPSTR1 affects COPS6
1 |
1 |

No evidence text available
GRB2 affects COPS6
1 |
1 |

No evidence text available
FOXI1 affects COPS6
1 |
1 |

No evidence text available
EGFR-ERK affects COPS6
| 1
EGFR-ERK activates COPS6. 1 / 1
| 1

reach
"Thus, these results suggest that the EGFR-ERK pathway may upregulate CSN6, which may inhibit PD-L1 degradation and subsequently maintain PD-L1 stability in GBM."
EGFR affects COPS6
| 1
EGFR inhibits COPS6. 1 / 1
| 1

reach
"Mechanistic studies have shown that CSN6 is deregulated by EGFR signaling, in which ERK2 interacts with CSN6."
DYNLL1 affects COPS6
1 |
1 |

No evidence text available
DOCK7 affects COPS6
1 |
1 |

No evidence text available
DCAF4L1 affects COPS6
1 |
1 |

No evidence text available
DCAF17 affects COPS6
1 |
1 |

No evidence text available
DCAF15 affects COPS6
1 |
1 |

No evidence text available
CUL affects csn
| 1
Csn binds CSN2, COPS6, CUL, and RBX1. 1 / 1
| 1

sparser
"CSN physically interacts with the cullin and RBX1 subunits of CRLs through its subunits CSN2 and CSN6 and it is thought that these interactions provide CSN with the affinity for its CRL targets ( xref )."
CUL affects RING-box protein 1
| 1
CSN2 binds RING-box protein 1, CUL, and COPS6. 1 / 1
| 1

sparser
"The CSN becomes assembled in supercomplexes with CRLs in which CSN2 interacts with cullins and CSN6 with Rbx1 [16,17] ."
CUL affects RBX1
| 1
Csn binds CSN2, COPS6, CUL, and RBX1. 1 / 1
| 1

sparser
"CSN physically interacts with the cullin and RBX1 subunits of CRLs through its subunits CSN2 and CSN6 and it is thought that these interactions provide CSN with the affinity for its CRL targets ( xref )."
| 1

sparser
"The core of the subcomplex is based on a stable heterotrimeric association of CSN7, CSN4, and CSN6, requiring coexpression in a bacterial reconstitution system."
CSN2 affects csn
| 1
Csn binds CSN2, COPS6, CUL, and RBX1. 1 / 1
| 1

sparser
"CSN physically interacts with the cullin and RBX1 subunits of CRLs through its subunits CSN2 and CSN6 and it is thought that these interactions provide CSN with the affinity for its CRL targets ( xref )."
CSN2 affects RING-box protein 1
| 1
CSN2 binds RING-box protein 1, CUL, and COPS6. 1 / 1
| 1

sparser
"The CSN becomes assembled in supercomplexes with CRLs in which CSN2 interacts with cullins and CSN6 with Rbx1 [16,17] ."
CSN2 affects RBX1
| 1
Csn binds CSN2, COPS6, CUL, and RBX1. 1 / 1
| 1

sparser
"CSN physically interacts with the cullin and RBX1 subunits of CRLs through its subunits CSN2 and CSN6 and it is thought that these interactions provide CSN with the affinity for its CRL targets ( xref )."
COPS6 affects ubiquitination degradation cancer associated proteins
| 1
COPS6 activates ubiquitination degradation cancer associated proteins. 1 / 1
| 1

eidos
"Furthermore , CSN6 can also coordinate with E3 ligase to mediate ubiquitination and degradation of cancer associated proteins 14 ."
COPS6 affects ubiquitin proteasome system-mediated Nkx2.2 protein
| 1
COPS6 inhibits ubiquitin proteasome system-mediated Nkx2.2 protein. 1 / 1
| 1

eidos
"Mechanistically , CSN6 blocked the ubiquitin proteasome system-mediated degradation of Nkx2.2 protein by interacting with it and inhibiting its ubiquitination directly in cardiomyocytes ."
COPS6 affects tumour-formation diluted
| 1
COPS6 activates tumour-formation diluted. 1 / 1
| 1

eidos
"Knockdown of CSN6 led to reduction of tumour-formation frequency in vivo at low diluted level ( Fig. 6a , b ) ."
COPS6 affects tumour growth
| 1
COPS6 activates tumour growth. 1 / 1
| 1

eidos
"We also found that knockdown of CSN6 reduced tumour growth ( Fig. 6c ) ."
COPS6 affects spheres enriched
| 1
COPS6 activates spheres enriched. 1 / 1
| 1

eidos
"CSN6 has the capability to promote the formation of spheres enriched in CSCs , suggesting that CSN6 overexpression may promote distant metastasis and confer resistance after chemotherapy ."
COPS6 affects soft agar colony
| 1
COPS6 activates soft agar colony. 1 / 1
| 1

eidos
"CSN6 knockdown hindered cell growth , soft agar colony formation , and tumorigenicity ( Figure S17A-D , Supporting Information ) compared with control cells ."

reach
"Furthermore, CSN6 prevents E3 ligase β-Trcp-mediated DDX5 proteasome degradation, which in turn stables PHGDH mRNA in a DDX5-dependent manner [111]."

reach
"In another study, quercetin inhibited the viability of HT29 cells, caused cell shrinkage, chromatin condensation, and nuclear collapse, lessened the protein expression levels of phosphorylated-Akt, and augmented the protein degradation of constitutive photomorphogenesis 6 signalosome (CSN6) (Yang et al., 2016)."
COPS6 affects paaD
| 1
COPS6 activates paaD. 1 / 1
| 1

reach
"Importantly, inhibition of CSN6 decreased the metastasis of PAAD to liver."
COPS6 affects metastasis potential breast cancer cells
| 1
COPS6 activates metastasis potential breast cancer cells. 1 / 1
| 1

eidos
"In summary , we draw noteworthy conclusions that CSN6 had a positive impact on Snail1 stability , and then contributing to the enhanced metastasis potential of breast cancer cells ."
COPS6 affects lactate fructose-1
| 1
COPS6 activates lactate fructose-1. 1 / 1
| 1

eidos
"Another metabolomic analysis by mass spectrometry demonstrated that CSN6 knockdown leads to reduction of lactate and fructose-1 ,6 - bisphosphate , and the significance of the changes in metabolite levels was documented ( Figure 5B , Figures S13 and S14 , Supporting Information ) ."
COPS6 affects hsdR
| 1
| 1

reach
"In vitro experiments revealed that hPACAP and hVIP also bind to hSR and activate downstream signaling, but only at high concentrations."
COPS6 affects growth migration invasion melanoma cells
| 1
COPS6 activates growth migration invasion melanoma cells. 1 / 1
| 1

eidos
"In this study , we show that CSN6 promotes the growth , migration and invasion of melanoma cells via CDK9-mediated signaling pathways ."
COPS6 affects glycine
| 1
COPS6 activates glycine. 1 / 1
| 1

eidos
"Metabolomic studies demonstrate that CSN6 expression leads to serine and glycine production ."
COPS6 affects decelerated turnover TRIM21
| 1
COPS6 inhibits decelerated turnover TRIM21. 1 / 1
| 1

eidos
"Indeed , CSN6 knockdown led to decelerated turnover of TRIM21 but accelerated turnover of OCT1 ( Fig. 4c ) ."
| 1

reach
"Then, we explored how CSN6 affects the stability of CDK9 and found that overexpression of CSN6 decreased the turnover rate of CDK9 in melanoma cells by using the de novo protein synthesis inhibitor cycloheximide (CHX, Sigma, USA)."
COPS6 affects cullin E3 ligases
| 1
COPS6 inhibits cullin E3 ligases. 1 / 1
| 1

eidos
"CSN6 inhibits activity of cullin E3 ligases by removing Nedd8 modifications to prevent desmosomal degradation / dissolution ."
COPS6 affects csn
| 1
Csn binds CSN2, COPS6, CUL, and RBX1. 1 / 1
| 1

sparser
"CSN physically interacts with the cullin and RBX1 subunits of CRLs through its subunits CSN2 and CSN6 and it is thought that these interactions provide CSN with the affinity for its CRL targets ( xref )."

reach
"Through combinatorial effects on the MDM2-p53 signaling axis and now SKP2 mediated p57 Kip2 degradation, it is hypothesized that CSN6 overexpression can effectively promote cell proliferation by simultaneously relieving cell cycle restraints, apoptosis, senescence, and presumably p53 functions in DNA damage repair and cell metabolism, among other important tumor suppressive mechanisms."
COPS6 affects cancer stemness
| 1
COPS6 activates cancer stemness. 1 / 1
| 1

eidos
"Our previous observation that CSN6 overexpression reduces the recurrence-free survival of CRC patients23 is probably , at least in part , due to the promotion of cancer stemness by CSN6 ."
COPS6 affects breast cancer cells
| 1
COPS6 activates breast cancer cells. 1 / 1
| 1

eidos
"Furthermore , we identified that CSN6 enhancement leads to Snail1 stabilization and promotes metastasis of breast cancer cells through inhibiting Snail1 ubiquitination ."
COPS6 affects activity
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COPS6 activates activity. 1 / 1
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sparser
"Because KRAS is an upstream regulator of these two pathways, KRAS mutations will continue to activate both RAS-MAPK and AKT pathways to stabilize CSN6, which in turn activates β-catenin activity to cause pathogenesis in colon cancer."
COPS6 affects ZYG11B
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No evidence text available
COPS6 affects ZMAT2
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No evidence text available
COPS6 affects Wnt
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COPS6 activates Wnt. 1 / 1
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"Downregulation of CSN6 attenuates papillary thyroid carcinoma progression by reducing Wnt and beta-catenin signaling and sensitizes cancer cells to FH535 therapy."
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eidos
"Consistent with this finding , knockdown of CSN6 reduces the ubiquitination level of TRIM21 and decelerated turnover of TRIM21 ( Fig. 4b , c ) ."
COPS6 affects USP9X
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No evidence text available
COPS6 affects UBE2H
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No evidence text available
COPS6 affects Trp-Gly
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COPS6 activates Trp-Gly. 1 / 1
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"Of note, CSN6 overexpression suppressed the Wg signaling defects caused by Axin overexpression (XREF_SUPPLEMENTARY B), but did not suppress Wg signaling defects caused by Lgs 17E overexpression (XREF_SUPPLEMENTARY E)."
COPS6 affects TXN
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No evidence text available
COPS6 affects TUBB
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No evidence text available
COPS6 affects TP63
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No evidence text available
COPS6 affects TEAD4
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No evidence text available
COPS6 affects TEAD
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COPS6 inhibits TEAD. 1 / 1
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"With transcriptional activity assay, we found that CSN6 ablation repressed YAP1‐TEAD transcriptional activity (Figure 2E)."
COPS6 affects SYNCRIP
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No evidence text available
COPS6 affects SNRNP70
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1 |

No evidence text available
COPS6 affects SMC3
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No evidence text available
COPS6 affects SCG2
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No evidence text available
COPS6 affects RPS4X
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No evidence text available
COPS6 affects RPS20
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No evidence text available
COPS6 affects RPLP2
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No evidence text available
COPS6 affects RPL11
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No evidence text available
COPS6 affects RPA1
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No evidence text available
COPS6 affects RING-box protein 1
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CSN2 binds RING-box protein 1, CUL, and COPS6. 1 / 1
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sparser
"The CSN becomes assembled in supercomplexes with CRLs in which CSN2 interacts with cullins and CSN6 with Rbx1 [16,17] ."
COPS6 affects RBM14
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No evidence text available
COPS6 affects PWP1
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No evidence text available
COPS6 affects PSMD14
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No evidence text available
COPS6 affects POP7
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No evidence text available
COPS6 affects PBX2
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No evidence text available
COPS6 affects PABPC1
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No evidence text available
COPS6 affects NHERF2
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No evidence text available
COPS6 affects Mice
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COPS6 activates Mice. 1 / 1
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"9e Besides, we also found that CSN6 promotes tumorigenesis in mice through directly stabilization of MDM2."
COPS6 affects MKI67
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COPS6 activates MKI67. 1 / 1
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"Further, CSN6 KD tumors demonstrate decreased FASN protein levels based on immunohistochemistry (IHC) staining (Fig. 6i) and immunoblotting (Supplementary Fig. 4h), low BODIPY staining (Fig. 6j) and relatively low levels of tumor cell growth (as measured by Ki67 staining) (Fig. 6i), while CSN6-reexpressing tumors can restore the levels of FASN (Fig. 6i, Supplementary Fig. 4h), increase BODIPY staining (Fig. 6j), and elevate Ki67 staining concurrently (Fig. 6i)."
COPS6 affects MFSD14A
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No evidence text available
COPS6 affects MBTD1
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No evidence text available
COPS6 affects M
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COPS6 binds M. 1 / 1
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No evidence text available
COPS6 affects LYZ
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No evidence text available
COPS6 affects LOC100128400
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COPS6 binds LOC100128400. 1 / 1
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No evidence text available
COPS6 affects LARP4
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No evidence text available
COPS6 affects KRT75
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No evidence text available
COPS6 affects KRT24
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No evidence text available
COPS6 affects KCTD9
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No evidence text available
COPS6 affects JUN
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COPS6 activates JUN. 1 / 1
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"CSN6 positively regulates c-Jun in a MEKK1 dependent manner."
COPS6 affects IRF8
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No evidence text available
COPS6 affects IGHV3OR15-7
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No evidence text available
COPS6 affects IDEF1
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COPS6 inhibits IDEF1. 1 / 1
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"Collectively, the cellular CSN6 level is decreased during early stages of -Fe to ensure the rapid accumulation of IDEF1, which in turn up-regulates several iron uptake and utilisation related genes to help overcome -Fe stress in rice."
COPS6 affects HSPA12A
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No evidence text available
COPS6 affects HSP90B1
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No evidence text available
COPS6 affects HSP90AB1
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No evidence text available
COPS6 affects HAPSTR1
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No evidence text available
COPS6 affects GRB2
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No evidence text available
COPS6 affects GOT
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COPS6 decreases the amount of GOT. 1 / 1
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"Also, Csn6 (HDTI) mice have decreased level of ALT, AST, LDH, cholesterol and TG in both CD and HFD conditions (Figure 7D)."
COPS6 affects FOXI1
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No evidence text available
COPS6 affects FBXW7beta
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COPS6 leads to the ubiquitination of FBXW7beta. 1 / 1
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"CSN6 enhances FBXW7beta autoubiquitination and degradation."
COPS6 affects EGFR-interacting E3 ligase CHIP
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COPS6 inhibits EGFR-interacting E3 ligase CHIP. 1 / 1
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eidos
"CSN6 may also destabilize EGFR-interacting E3 ligase CHIP by promoting its autoubiquitination ( 278 ) ."
COPS6 affects DYNLL1
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No evidence text available
COPS6 affects DOCK7
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No evidence text available
COPS6 affects DDX5
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COPS6 activates DDX5. 1 / 1
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"Furthermore, CSN6 prevents E3 ligase β-Trcp-mediated DDX5 proteasome degradation, which in turn stables PHGDH mRNA in a DDX5-dependent manner [111]."
COPS6 affects DCAF4L1
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No evidence text available
COPS6 affects DCAF17
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No evidence text available
COPS6 affects DCAF15
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No evidence text available
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sparser
"The core of the subcomplex is based on a stable heterotrimeric association of CSN7, CSN4, and CSN6, requiring coexpression in a bacterial reconstitution system."
COPS6 affects CSN6-CSN5 hetreodimer
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COPS6 inhibits CSN6-CSN5 hetreodimer. 1 / 1
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"In competition experiment, more CSN6 may leads to reduced CSN6-CSN5 hetreodimer, thereby affecting Culllin deneddylation 55."
COPS6 affects CSN2, CUL, and RING-box protein 1
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CSN2 binds RING-box protein 1, CUL, and COPS6. 1 / 1
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sparser
"The CSN becomes assembled in supercomplexes with CRLs in which CSN2 interacts with cullins and CSN6 with Rbx1 [16,17] ."
COPS6 affects CSN2, CUL, RBX1, and csn
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Csn binds CSN2, COPS6, CUL, and RBX1. 1 / 1
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sparser
"CSN physically interacts with the cullin and RBX1 subunits of CRLs through its subunits CSN2 and CSN6 and it is thought that these interactions provide CSN with the affinity for its CRL targets ( xref )."
COPS6 affects COA1
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No evidence text available
COPS6 affects CIB2
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COPS6 inhibits CIB2. 1 / 1
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"By contrast, CSN6 knockdown impaired SKP2 mediated polybiquitylation of p57 Kip2."
COPS6 affects CDKN1A
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COPS6 increases the amount of CDKN1A. 1 / 1
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"Further, COPS6 or COPS9 overexpression enhanced the expression levels of cell cycle regulators CyclinB1 and CDK4, but downregulated the expression of cyclin-dependent kinase inhibitors p18 and p21 according to western blot analysis (Figure 11D)."
COPS6 affects CDK4
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No evidence text available

reach
"Furthermore, COPS6 was found to inhibit CD8+ T-cell infiltration in the tumor microenvironment, which facilitates tumor immune escape."
COPS6 affects BFSP2
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No evidence text available
COPS6 affects Ad
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sparser
"On the contrary, up-regulation of CSN6 by transfecting CSN6 overexpressed adenovirus (Ad-CSN6) aggravated an Ang II-induced increase of cardiomyocyte size and hypertrophic marker genes’ expression ( F[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
COPS6 affects ANXA1
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No evidence text available
COPS6 affects AMOTL2
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No evidence text available
COPS6 affects ABCC6
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No evidence text available
COPS6 affects A375
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COPS6 binds UBR5 and A375. 1 / 1
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"The E3 ubiquitin ligase UBR5 has shown the ability to ubiquitinate CDK9 38, so we hypothesized that CSN6 stabilizes the expression of CDK9 by regulating the E3 ligase UBR5.A co-IP assay was performed and demonstrated that CSN6 interacted with UBR5 in A375 and MV3 melanoma cells."

reach
"Consistent with this, flow cytometric studies also showed that CSN6 KD decreased the BODIPY staining when compared with related controls (Fig. 1f)."
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sparser
"The core of the subcomplex is based on a stable heterotrimeric association of CSN7, CSN4, and CSN6, requiring coexpression in a bacterial reconstitution system."
COA1 affects COPS6
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No evidence text available
CHX affects COPS6
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CHX activates COPS6. 1 / 1
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"When the cells were treated with EGF and cycloheximide (CHX), a protein synthesis inhibitor, EGF reduced CHX induced CSN6 and PD-L1 turnover in GBM cells."
CDK4 affects COPS6
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No evidence text available
BFSP2 affects COPS6
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No evidence text available
Akt inhibitor affects COPS6
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Akt inhibitor increases the amount of COPS6. 1 / 1
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"PI3K and Akt inhibitor L294002 reduced the S60 phosphorylation level of CSN6 and caused subsequent E6AP downregulation."
Ad affects COPS6
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sparser
"On the contrary, up-regulation of CSN6 by transfecting CSN6 overexpressed adenovirus (Ad-CSN6) aggravated an Ang II-induced increase of cardiomyocyte size and hypertrophic marker genes’ expression ( F[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
ANXA1 affects COPS6
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No evidence text available
AMOTL2 affects COPS6
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No evidence text available
ALDOA affects COPS6
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ALDOA activates COPS6. 1 / 1
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"To summarize, aberrant ALDOA facilitated the EMT process, activated the MAPK signaling cascade of CRC cells by targeting COPS6, and ultimately accelerated CRC proliferation and metastasis."
ABCC6 affects COPS6
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No evidence text available
A375 affects UBR5
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COPS6 binds UBR5 and A375. 1 / 1
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"The E3 ubiquitin ligase UBR5 has shown the ability to ubiquitinate CDK9 38, so we hypothesized that CSN6 stabilizes the expression of CDK9 by regulating the E3 ligase UBR5.A co-IP assay was performed and demonstrated that CSN6 interacted with UBR5 in A375 and MV3 melanoma cells."

No evidence text available