IndraLab

Statements


VCP affects ATXN3
17 2 | 113 108
VCP binds ATXN3.
17 2 | 89 108
17 2 | 85 105

sparser
"However, the mildly increased interaction between p97 and ATX3 after IR observed by the SILAC mass spectrometry was not very clear when analysed by Western blotting."

reach
"VCP and p97 also interacts with ATXN3 and SPA3 (see above), another deubiquitinating enzyme."

sparser
"Under physiological conditions, RNF8 catalyzes its own K48-linked ubiquitination, which is antagonized by the p97-ATX3 complex, contributing to preservation of RNF8 abundance."

sparser
"The p97ATX3 complex safeguards the soluble pool of RNF8 under physiological conditions."

sparser
"Fig. 1 E shows external eye photos of flies expressing ataxin-3 that do not bind VCP."

reach
"The 282 RKRR-HNHH substitution disrupts the interaction of VCP and p97 with wild-type and expanded ataxin-3, and blocks VCP and p97 activation of ataxin-3 DUB activity."

sparser
"Overall, this shows that the p97ATX3 complex extracts RNF8 from sites of DNA damage to facilitate the timely ubiquitination of H1 and recruitment of downstream factors RNF168/53BP1."

sparser
"For example, in SCA3, it was shown that changes in the interaction between ataxin-3 and valosin-containing protein (VCP/p97) leads to dysfunctions in ataxin-3 function as a regulator of endoplasmic reticulum-associated degradation xref ."

sparser
"In xref and xref , we used mild conditions that brought down some VCP non-specifically, which helped us to not exclude the possibility of residual VCP interaction with VCP-binding-mutated ataxin-3."

reach
"We conclude that interaction between UbD2 and p97 and Atx3 mediates retranslocation of UbD2 to the cytoplasm for terminal degradation in the proteasomes, a pathway that is accelerated by exposure to T4."
| 3

sparser
"Ataxin-3 interacts with ubiquitinated substrates, p97, Rad23, and the proteasome, and the expanded-polyglutamine-containing ataxin-3 is defective in the substrate degradation [74] ."

sparser
"Ataxin-3 binds directly to at least two PQC-related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; xref )."

sparser
"Ataxin-3 binds directly to at least two PQC-related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; Fig. 1 A)."
VCP binds ATXN3 and arginine. 2 / 2
| 2

reach
"VCP binds ataxin-3 at an arginine rich region that precedes its polyQ portion (XREF_FIG)."

reach
"VCP is bound directly by ataxin-3 through an arginine rich area preceding the polyQ repeat."
VCP binds mutated ATXN3. 2 / 2
| 2

reach
"Mutant ataxin-3 binds the ERAD component VCP and p97 more efficiently than wild-type ataxin-3, probably because of conformational changes [XREF_BIBR, XREF_BIBR, XREF_BIBR]."

reach
"Mutant ataxin-3 binds the ERAD component VCP and p97 more efficiently than wild-type ataxin-3, probably because of conformational changes [XREF_BIBR, XREF_BIBR, XREF_BIBR]."
VCP activates ATXN3.
| 22
VCP activates ATXN3. 10 / 23
| 22

reach
"An arginine and lysine rich motif is crucial for VCP and p97 mediated modulation of ataxin-3 fibrillogenesis."

reach
"The 282 RKRR-HNHH substitution disrupts the interaction of VCP and p97 with wild-type and expanded ataxin-3, and blocks VCP and p97 activation of ataxin-3 DUB activity."

reach
"The same study also showed that recombinant VCP stimulates fibrillogenesis and aggregation of recombinant, pathogenic ataxin-3 in a dose dependent manner in reconstituted systems, but only up to a point; molar excess VCP suppresses ataxin-3 fibrillogenesis."

reach
"Valosin Containing Protein (VCP and p97) Is an Activator of Wild-Type Ataxin-3."

reach
"This result validates the direct VCP stimulation of wild-type ataxin-3."

reach
"VCP and p97 does not enhance ubiquitin hydrolase activity of expanded ataxin-3."

reach
"In conclusion, we observe decreased toxicity from ataxin-3 when VCP is knocked down in fly eyes, indicating that VCP enhances ataxin-3 aggregation and toxicity."

reach
"VCP and p97 enhances wild-type ataxin-3 activity in vitro."

reach
"Here, we sought to investigate whether VCP and p97 or hHR23A, both of which are involved in protein degradation pathways, also enhanced ataxin-3 activity through direct interactions."

reach
"As shown in XREF_FIG, no reaction products were detected, demonstrating that VCP and p97 enhances ataxin-3 enzymatic activity rather than act directly on ubiquitin chains."
VCP inhibits ATXN3.
| 2
VCP inhibits ATXN3. 2 / 2
| 2

reach
"The same study also showed that recombinant VCP stimulates fibrillogenesis and aggregation of recombinant, pathogenic ataxin-3 in a dose dependent manner in reconstituted systems, but only up to a point; molar excess VCP suppresses ataxin-3 fibrillogenesis."

reach
"Nevertheless, interaction with hHR23A could change the affinity of ataxin-3 for VCP and p97 and thus impair VCP and p97 induced activation of ataxin-3."
ATXN3 affects VCP
17 2 1 | 96 108
ATXN3 binds VCP.
17 2 | 89 108
17 2 | 85 105

sparser
"However, the mildly increased interaction between p97 and ATX3 after IR observed by the SILAC mass spectrometry was not very clear when analysed by Western blotting."

reach
"VCP and p97 also interacts with ATXN3 and SPA3 (see above), another deubiquitinating enzyme."

sparser
"Under physiological conditions, RNF8 catalyzes its own K48-linked ubiquitination, which is antagonized by the p97-ATX3 complex, contributing to preservation of RNF8 abundance."

sparser
"The p97ATX3 complex safeguards the soluble pool of RNF8 under physiological conditions."

sparser
"Fig. 1 E shows external eye photos of flies expressing ataxin-3 that do not bind VCP."

reach
"The 282 RKRR-HNHH substitution disrupts the interaction of VCP and p97 with wild-type and expanded ataxin-3, and blocks VCP and p97 activation of ataxin-3 DUB activity."

sparser
"Overall, this shows that the p97ATX3 complex extracts RNF8 from sites of DNA damage to facilitate the timely ubiquitination of H1 and recruitment of downstream factors RNF168/53BP1."

sparser
"For example, in SCA3, it was shown that changes in the interaction between ataxin-3 and valosin-containing protein (VCP/p97) leads to dysfunctions in ataxin-3 function as a regulator of endoplasmic reticulum-associated degradation xref ."

sparser
"In xref and xref , we used mild conditions that brought down some VCP non-specifically, which helped us to not exclude the possibility of residual VCP interaction with VCP-binding-mutated ataxin-3."

reach
"We conclude that interaction between UbD2 and p97 and Atx3 mediates retranslocation of UbD2 to the cytoplasm for terminal degradation in the proteasomes, a pathway that is accelerated by exposure to T4."
| 3

sparser
"Ataxin-3 interacts with ubiquitinated substrates, p97, Rad23, and the proteasome, and the expanded-polyglutamine-containing ataxin-3 is defective in the substrate degradation [74] ."

sparser
"Ataxin-3 binds directly to at least two PQC-related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; xref )."

sparser
"Ataxin-3 binds directly to at least two PQC-related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; Fig. 1 A)."
VCP binds ATXN3 and arginine. 2 / 2
| 2

reach
"VCP binds ataxin-3 at an arginine rich region that precedes its polyQ portion (XREF_FIG)."

reach
"VCP is bound directly by ataxin-3 through an arginine rich area preceding the polyQ repeat."
VCP binds mutated ATXN3. 2 / 2
| 2

reach
"Mutant ataxin-3 binds the ERAD component VCP and p97 more efficiently than wild-type ataxin-3, probably because of conformational changes [XREF_BIBR, XREF_BIBR, XREF_BIBR]."

reach
"Mutant ataxin-3 binds the ERAD component VCP and p97 more efficiently than wild-type ataxin-3, probably because of conformational changes [XREF_BIBR, XREF_BIBR, XREF_BIBR]."
ATXN3 activates VCP.
| 5
ATXN3 activates VCP. 5 / 5
| 5

reach
"In contrast, evidence has also shown that ataxin-3 may promote p97 associated deubiquitination to facilitate the transfer of ERAD substrates to the proteasome XREF_BIBR."

reach
"Various ataxin-3 protein domains contribute to its toxicity, including the valosin-containing protein (VCP)-binding motif (VBM)."

reach
"In this scenario, ATX3 promotes sufficient p97‐dependent extraction of RNF8 in a classical ERAD‐like manner (Wang et al, 2006; Zhong & Pittman, 2006)."

reach
"We present evidence that atx3 may promote p97 associated deubiquitination to facilitate the transfer of polypeptides from p97 to the proteasome."

reach
"Atx3 promotes p97 associated deubiquitination."
ATXN3 deubiquitinates VCP.
1 | 2
ATXN3 leads to the deubiquitination of VCP. 3 / 3
1 | 2

reach
"Ataxin-3 and YOD1 promote the deubiquitination of p97 associated ERAD substrates, and facilitate delivery to the proteasome [21-24]."

reach
"We propose that atx3 may promote deubiquitination of p97 bound substrates to facilitate their transfer to the proteasome during retrotranslocation (XREF_FIG)."

"We present evidence that atx3 may promote p97-associated deubiquitination to facilitate the transfer of polypeptides from p97 to the proteasome."
ATXN3 is modified
| 1 118 10
ATXN3 is ubiquitinated.
| 1 65
ATXN3 is ubiquitinated. 10 / 66
| 1 65

sparser
"Interestingly, this ubiquitin ligase also mediates ubiquitination of spinocerebellar ataxia-associated ataxin-3 [ xref ]. gp78 overexpression promotes the ubiquitination and degradation of SOD1 and ataxin-3 in cultured cells, whereas knockdown of gp78 stabilizes them [ xref ]."

sparser
"We explored this notion in this study and present evidence that: (a) the C-terminus of ataxin-3 isoform 2 signals its degradation in a proteasome-dependent manner, (b) this effect from the C-terminus of isoform 2 does not require the ubiquitination of ataxin-3, and (c) the isolated C-terminus of isoform 2 can enhance the degradation of an unrelated protein."

sparser
"Based on these previously published findings and our present results, we propose that ubiquitination of ataxin-3 serves to regulate its DUB functions, rather than directly dictate its proteasomal turnover."

sparser
"One possibility through which this interaction could occur would be through ataxin-3 ubiquitination."

reach
"Furthermore, UFD2a, a mammalian ubiquitin-chain assembly factor (E4), associated with VCP and induced polyubiquitylation of MJD1."

sparser
"Ube4b showed no difference in the level of ubiquitination in normal or pathological ataxin3, which has an expanded polyglutamine tract; however, this process was shown to be mediated by VCP proteins [ xref , xref ]."

sparser
"In these latter studies ubiquitination, including on K117, did not increase the degradation of ataxin-3; instead, the proposed mechanism was one whereby ubiquitinated ataxin-3 is more active as an enzyme and able to better protect against polyQ toxicity by enhancing the production of heat shock proteins ( xref , xref ; xref )."

sparser
"On the other hand, HSJ1a can also impede the proteasomal degradation of the ubiquitinated Atx3 to maintain the protein level when needed."

sparser
"The “K-null” constructs are based on extensive earlier work in which we showed that lysine-to-arginine replacements lead to ataxin-3 protein that is not ubiquitinated in vitro , in mammalian cells, or in vivo in Drosophila ( xref ; xref ; xref )."

sparser
"Although this post-translational modification occurs in a UIM-dependent manner, it becomes independent of UIMs when the catalytic cysteine residue of ataxin-3 is mutated, suggesting that ataxin-3 ubiquitination is itself regulated in trans by its own de-ubiquitinating activity."
ATXN3 is phosphorylated.
| 42 10
ATXN3 is phosphorylated. 10 / 40
| 32 8

rlimsp
"Moreover, we found that ATXN3 shifted to the nucleus upon thermal stress in a CK2-dependent manner, indicating a key role of CK2-mediated phosphorylation of ATXN3 in SCA3 pathophysiology."

sparser
"We show NOD2 and TLR2 mediate phosphorylation of the deubiquitinase ataxin-3 via RIPK2 and TBK1."

sparser
"With respect to SCA3 it is known to phosphorylate ataxin-3 and thus regulate its aggregation propensity [ xref , xref ]."

sparser
"Nonetheless, further examination of drug effects on ataxin-3 phosphorylation status and aggregation potential is warranted."

sparser
"ATX3 phosphorylation may target pathological ATX3 to the nucleus, where it eludes cytoplasmic ubiquitination and proteasomal degradation and forms nuclear aggregates [ xref ]."

sparser
"For mapping the site(s) in ataxin-3 that is phosphorylated by GSK 3β, we analyzed the potential phosphorylation motifs in ataxin-3. (S/T)XXX(S/T) is a consensus phosphorylation motif preferred by GSK [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Recent studies implicate the third UIM in casein kinase 2-dependent ataxin-3 phosphorylation xref , a modification that influences nucleocytoplasmic shuttling and intranuclear aggregation of ataxin-3."

rlimsp
"Ataxin-3 is, for instance, able to repress matrix metalloproteinase-2 (MMP-2) transcription, and improved nuclear localisation of ataxin-3 through phosphorylation enhances this transcriptional repression [30]."

sparser
"Additionally, studies demonstrated that phosphorylation of Ataxin3 influences its aggregation and counters the neuromorphologic defects occurring due to it by decreasing its deubiquitinase activity ( xref ; xref )."

sparser
"However, the kinase that phosphorylates ataxin-3 remains unknown."
ATXN3 is phosphorylated on S256. 8 / 8
| 8

sparser
"Phosphorylation has also been implicated in influencing the toxicity of SCA3, DRPLA and SBMA, for example: phosphorylation of ataxin-3 at S256 by glycogen synthase kinase 3β reduces aggregation of polyglutamine-expanded atxain-3 xref ; phosphorylation of polyglutamine-expanded androgen receptor by MAPK at S516 is associated with increased cleavage and toxicity in cell models of SBMA xref ; and S734 of atrophin-1 is phosphorylated by Jun-N-terminal kinase, although the significance of this in the pathogenesis of DRPLA has not yet been examined."

sparser
"In our present study, we demonstrate that GSK 3β phosphorylates ataxin-3 at serine 256 ."

sparser
"For example, ATX3 phosphorylation at Ser256 by GSK3β regulates ATX3 aggregation [ xref ]."

sparser
"Inhibition of S256 phosphorylation in normal ATX3 does not change its aggregation ability, but greatly increases its self-aggregation in expanded ATX3."

sparser
"Further studies to examine the levels of phosphorylated species of normal and expanded ataxin-3, as well as the activities of GSK 3β, in MJD brain or transgenic animals, will be of benefit for underst[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"As the aggregate formation is a pathological hallmark in MJD, we wonder if phosphorylation of ataxin-3 at serine 256 by GSK 3β affects the aggregate formation."

sparser
"These data suggest that preventing phosphorylation of S256 in normal ataxin-3 does not change ataxin-3 aggregation ability, but in expanded ataxin-3, greatly increases itself aggregation."

sparser
"As shown in C, conversion of the S256 to alanine in ataxin-3 completely abolished its phosphorylation by GSK 3β, whereas other mutants were still phosphorylated by GSK 3β, suggesting that GSK 3β phosp[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
ATXN3 is phosphorylated on S12. 4 / 4
| 2 2

sparser
"Matos et al. found that ataxin-3 is phosphorylated on serine 12 in cultured rat neurons, and mutating this serine to a phosphomimetic aspartate residue inhibited the enzyme’s deubiquitinase activity."
| PMC

rlimsp
"We report that S12 of ataxin-3 is phosphorylated in neurons and that mutating this residue so as to mimic a constitutive phosphorylated state counters the neuromorphologic defects observed. In rats stereotaxically injected with expanded ataxin-3-encoding lentiviral vectors, mutation of serine 12 reduces aggregation, neuronal loss, and synapse loss. Our results suggest that S12 plays a role in the pathogenic pathways mediated by polyglutamine-expanded ataxin-3 and that phosphorylation of this residue protects against toxicity."

sparser
"The phosphorylation of Ataxin3 at the Ser12 residue adjacent to the catalytic domain can also counter the neuromorphological defects caused by the decrease in deubiquitinase activity."

rlimsp
"We report that S12 of ataxin-3 is phosphorylated in neurons and that mutating this residue so as to mimic a constitutive phosphorylated state counters the neuromorphologic defects observed."
ATXN3 is sumoylated.
| 11
ATXN3 is sumoylated. 10 / 11
| 11

sparser
"A plausible explanation for this discrepancy is that SUMOylated ataxin-3 is susceptible to proteolysis and thus difficult to detect unless proteolytic activities are compromised."

sparser
"However, a similar study demonstrated the presence of the SUMOylated ataxin-3 by employing affinity isolation and TCA precipitation (Zhou et al. xref )."

sparser
"Our findings revealed the role of ataxin-3 SUMOylation in SCA3/MJD pathogenesis."

sparser
"We tried again to detect SUMOylated ataxin-3 in the presence of 3-MA; however, the attempts were still unsuccessful (data not shown) suggesting that only a small fraction of ataxin-3 proteins may be SUMOylated."

sparser
"This process is antagonized by SUMOylation of ATXN3 [70] ."

sparser
"Ataxin-3 is SUMOylated and the SUMOylation of ataxin-3 regulates its function [ xref ]."

sparser
"SUMOylation Process of Ataxin-3."

sparser
"For example, SUMOylation reduces polyQ toxicity in HD, SCA1, and SCA7 ( xref ; xref ; xref ; xref ; xref ); however, SUMOylation of ataxin-3 increases its affinity to VCP, a protein that enhances ataxin-3 aggregation and exacerbates toxicity ( xref ; xref ; xref ; xref ; xref ; xref )."

sparser
"In addition, ATXN3 SUMOylation by SUMO-1 on site K166 also increases apoptosis in SCA3; therefore, SUMOylation by SUMO-1 might stimulate SCA3 pathogenesis through both effects described above."

sparser
"SUMOylation on Ataxin-3 has been reported to partially enhance its stability but produce no effects on its subcellular localization [ xref ]."
ATXN3 affects ATXN3
| 1 88
ATXN3 activates ATXN3.
| 1 78
ATXN3 activates ATXN3. 10 / 72
| 1 65

reach
"Non pathogenic ataxin-3 has a polyQ stretch less than 40 glutamine repeats, whereas pathogenic ataxin-3 causing SCA3 has that more than 60 glutamine repeats."

reach
"Machado-Joseph disease (MJD), an autosomal dominant type of spinocerebellar degeneration (SCD), is caused by CAG expansions in the MJD1 gene at chromosome 14q32.1 (Kawaguchi et al., 1994)."

reach
"SCA3, which is also known as Machado-Joseph disease (MJD), is caused by abnormal polyQ expansion in the deubiquitinase (DUB) ataxin-3."

reach
"SCA3 is caused by a CAG expansion in the ATXN3 gene for the protein ataxin-3 [XREF_BIBR] with a pathologic expansion number from 52 to 86 [XREF_BIBR]."

reach
"For instance, the ATXN3 gene usually contains 13-41 CAG repeats [XREF_BIBR]; more than 55 CAG repeats in the ATXN3 gene are pathogenic and can cause spinocerebellar ataxia type 3 (SCA3), which is a condition characterized by progressive problems with movement [XREF_BIBR]."

reach
"SCA3 is caused by a polyQ expansion in the carboxy-terminal portion of a cytosolic protein ataxin-3 (Atxn3) and primarily affects dentate and pontine nuclei and substantia nigra."

reach
"SCA3, considered to be the most common dominantly inherited ataxia in the world, is caused by an abnormal CAG expansion in the ATXN3 gene that is normally 12-42 repeats in length, but is expanded to ~ 52-84 repeats in diseased individuals 1."

reach
"SCA3, which is also known as Machado-Joseph disease (MJD), is caused by abnormal polyQ expansion in the deubiquitinase (DUB) ataxin-3 (Costa Mdo and Paulson, 2012; Matos et al., 2011)."

reach
"Taking into account the level of ataxin-3 overexpression in the homozygous SCA3 mouse that was used, the lead AON is an exciting candidate for further clinical advancement [213]."

eidos
"The ATXN3 gene , which causes SCA3 , also known as Machado-Joseph Disease ( MJD ) , contains a CAG repeat that is expanded in disease ."
Mutated ATXN3 activates ATXN3. 10 / 11
| 11

reach
"Mutant ATXN3 activates the pro apoptotic p53 pathway by activating ATM in SCA3."

reach
"Recent studies have reported that depletion of the mutant ATXN3 allele in a SCA3 transgenic mouse brains rescues the molecular phenotypes of SCA3 supporting the hypothesis that mutant ATXN3 elicits toxicity and neuronal dysfunction in SCA3 [XREF_BIBR]."

reach
"Mutant ATXN3 induces genomic DNA damage in SCA3."

reach
"Nonetheless, a significant understanding of the disease etiology of SCA3, the molecular mechanism by which the polyQ expansions in the mutant ATXN3 induce neurodegeneration in SCA3 has remained elusive."

reach
"As expected, the antibody combination 1H9 and MW1 shows a mutant ataxin-3 specific signal in SCA3 transgenic mice, whereas the antibodies 2B7 and MW1 specifically bind to mutant huntingtin in transgenic Huntington mice (XREF_FIG)."

reach
"Machado-Joseph disease (MJD), also called spinocerebellar ataxia type 3, is caused by mutant ataxin-3 with a polyglutamine expansion."

reach
"Mutant ATXN3 activates the DNA damage response pathway in SCA3."

reach
"While many studies indicate that SCA3 disease is predominantly caused by the mutant ATXN3 protein, SCA3 rCAG exp RNA has also been implicated in SCA3 pathogenesis."

reach
"Autophagy also has a role in clearance of other polyglutamine expanded proteins, including mutant ataxin-3 that is causing the spinocerebellar ataxia type 3 (SCA3) [XREF_BIBR]."

reach
"Spinocerebellar ataxia type 3 (SCA3) or Machado-Joseph disease is an autosomal dominant neurodegenerative disease and is caused by the mutation of ATXN3 gene that encodes ataxin-3 (Kawaguchi et al., 1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
ATXN3 activates mutated ATXN3. 2 / 2
| 2

reach
"We then proceeded to assess mutant ataxin-3 detection in crude brain homogenates of 6 months old wildtype and SCA3 transgenic mice overexpressing human mutant ataxin-3."

reach
"In SCA3, ATXN3 can be SUMOylated at site K166 by SUMO-1, which would enhance mutant ATXN3 stability without affecting aggregate formation."
ATXN3 inhibits ATXN3.
| 7
ATXN3 inhibits ATXN3. 4 / 9
| 4

reach
"In contrast to the findings of XREF_BIBR, polyQ expanded ataxin-3 was found to impair histone acetyltransferase activity in SCA3 mice, resulting in histone hypoacetylation."

reach
"However, whether ATXN3 loss-of-function contributes to SCA3 transcriptional dysfunction is still unknown."

reach
"The specific mechanism through which calpain inhibitor treatments increase autophagic activity in our transgenic zebrafish model of SCA3 is yet to be fully elucidated.These findings indicate that treatment with BLD-2736 may produce beneficial effects in SCA3 zebrafish via either decreasing cleavage of the ataxin-3 protein and/or increased autophagic clearance of any neurotoxic ataxin-3 protein aggregates."
| PMC

reach
"Phosphorylations of ATX3 by protein casein kinase 2 (CK2) stimulate SCA3 pathogenesis by altering its stability, nuclear localization, and inclusion formation [50,51], while GSK3β-mediated phosphorylation inhibits ATX3 aggregation which has a protective role in SCA3 pathophysiology [94]."
ATXN3 inhibits mutated ATXN3. 3 / 3
| 3

reach
"Oral administration of the calpain inhibitor BDA-410 decreased both fragments formation and full-length ataxin-3 levels, reduced aggregation of mutant ataxin-3 and prevented cell injury and striatal and cerebellar degeneration."

reach
"Additionally, in a doxycycline-regulatable transgenic mouse model of SCA3, reducing production of mutant ATXN3 transcripts via doxycycline treatment beginning at 9weeks eliminated disease features."

reach
"Recently, treatment of a C. elegans model of SCA3 (spinocerebellar ataxia type 3; also known as Machado-Joseph disease) with 17-(allylamino)-17-demethoxygeldanamycin (17-AAG), an HSP90 inhibitor, successfully decreased the mutant ATXN3 aggregation and improved locomotor activity [XREF_BIBR]."
ATXN3 decreases the amount of ATXN3.
| 3
ATXN3 decreases the amount of ATXN3. 3 / 3
| 3

reach
"Indeed, exogenous FBXL3 in SCA3 NPCs reduced the levels of both wild-type and pathogenic ATXN3 in a SCF dependent manner."

reach
"ATXN3 associated with CK2alpha and pharmacological inhibition of CK2 decreased nuclear ATXN3 levels and the formation of nuclear inclusions."

reach
"The simultaneous upregulation of closely related miRNAs targeting the 3 ' UTR of ATXN3 correlates with significantly reduced ATXN3 expression levels in SCA3-LCs suggesting that members of the miR-25 and miR-181 family cooperatively bind to the 3 ' UTR of ATXN3 to suppress the expression of ATXN3 in SCA3-LCs."
ATXN3 affects PNKP
2 | 1 38 34
ATXN3 binds PNKP.
2 | 22 32
2 | 10 32

sparser
"Our data in the accompanying manuscript by Chatterjee et al suggest that mutant ATXN3 binds PNKP and inhibits its 3’-phosphatase activity (Chatterjee et al, Figs. xref and xref )."

sparser
"We have made the unexpected observation that PNKP stably associates with Ataxin-3 (ATXN3), a polyglutamine repeat-containing protein mutated in spinocerebellar ataxia type 3 (SCA3), also known as Machado-Joseph Disease (MJD)."

sparser
"Together, these data support our previous interpretation that both wild-type and mutant ATXN3 interact with PNKP in the cell (Chatterjee et al)."

sparser
"The PLA analysis showed no significant interaction of ATXN3 with DNA LIG 3α in the brain sections from SCA3 patients, or in control brains under identical experimental conditions ( xref ; panels 1 and 4), suggesting specificity of the interactions between ATXN3 and PNKP in vivo ."

sparser
"Two studies in 2015 showed that ATXN3 binds to and enhances PNKP 3’-phosphatase activity, promoting DNA repair ( xref ; xref )."

reach
"To further confirm the interaction of PNKP and ATXN3 in cell, we performed bi-molecular fluorescence complementation (Bi-FC) assays, a versatile method to assess in cell protein protein interactions [XREF_BIBR]."

sparser
"To examine ATXN3’s association with PNKP, we immunoprecipitated (IP’d) PNKP and ATXN3 separately from the nuclear extract (NE, benzonase treated to remove DNA and RNA to avoid DNA-mediated co-immunoprecipitation) of human HEK-293 (human embryonic kidney cell line) ( xref ) and SH-SY5Y (a human neuroblastoma cell line) cells ( xref ) using the respective anti-protein (PNKP or ATXN3) antibody (Ab)."

sparser
"Although Chatterjee et al. found that polyQ-ataxin-3 does not interact with the phosphatase domain of PNKP in vitro, could mutant ataxin-3 nonetheless interact with PNKP in its native complex to inactivate its phosphatase activity?"

sparser
"Interestingly, the PNKPATXN3 interaction predominantly occurs outside of the nucleus in SCA3 patient brains, and the authors document colocalization of PNKP with polyQ-containing aggregates in SCA3 patient brains."

sparser
"We report that human polynucleotide kinase 3’-phosphatase (PNKP), a major DNA strand break repair enzyme, stably associates with Ataxin-3 (ATXN3)."
Mutated ATXN3 binds PNKP. 10 / 12
| 12

reach
"In the present study, we show that the essential DNA strand break repair enzyme PNKP (polynucleotide kinase 3 '-phosphatase) interacts with, and is inactivated by, the mutant ATXN3, resulting in inefficient DNA repair, persistent accumulation of DNA damage and strand breaks, and subsequent chronic activation of the DNA damage response ataxia telangiectasia mutated (ATM) signaling pathway in SCA3."

reach
"Here we have clearly shown that both WT and mutant ATXN3 interact directly with PNKP."

reach
"Studies of other polyQ diseases suggest that interactions in the soluble phase are an important aspect of the pathogenic cascade; hence, it is also possible that mutant ataxin-3 deleteriously interacts with PNKP independent of aggregate sequestration (XREF_FIG)."

reach
"These data substantiate our interpretation that both wild-type and mutant ATXN3 interact with PNKP in the cell."

reach
"Together, these data support our previous interpretation that both wild-type and mutant ATXN3 interact with PNKP in the cell (Chatterjee et al)."

reach
"Further, GST pull-down from the nuclear extract, followed by Western blot analysis, indicated that both wild-type and mutant ATXN3 directly interact with PNKP in vitro, (Chatterjee et al; XREF_FIG)."

reach
"In contrast, mutant ATXN3 interacts with PNKP and abrogates its 3 '-phosphatase activity, resulting in increased accumulation of DNA damage that chronically activates ATM --> p53 and ATM --> c-Abl --> PKCdelta pro apoptotic signaling to trigger neuronal dysfunction and apoptosis in SCA3 (illustrated in XREF_FIG)."

reach
"Although Chatterjee et al. found that polyQ-ataxin-3 does not interact with the phosphatase domain of PNKP in vitro, could mutant ataxin-3 nonetheless interact with PNKP in its native complex to inactivate its phosphatase activity?"

reach
"Our data in the accompanying manuscript by Chatterjee et al suggest that mutant ATXN3 binds PNKP and inhibits its 3 '-phosphatase activity (Chatterjee et al, Figs."

reach
"PNKP interacts with the mutant ATXN3 and is sequestered into the ATXN3-polyQ aggregates in SCA3 brain."
ATXN3 inhibits PNKP.
| 11 2
ATXN3 inhibits PNKP. 6 / 8
| 4 2

reach
"Diminished PNKP activity results in persistent accumulation of DNA strand breaks, leading to chronic activation of the DNA damage response ataxia telangiectasia mutated (ATM) protein kinase and the downstream pro apoptotic p53 dependent signaling pathways in SCA3."

sparser
"Hence, PNKP inactivation by expanded ataxin-3 may serve an important role in SCA3 pathogenesis, especially given the fact that the nervous system is particularly sensitive to DNA damage compared to other tissues xref ."

reach
"Hence, PNKP inactivation by expanded ataxin-3 may serve an important role in SCA3 pathogenesis, especially given the fact that the nervous system is particularly sensitive to DNA damage compared to other tissues XREF_BIBR."

reach
"Recently, two research teams reported the interaction between ATX3 and PNKP, and discovered that polyQ expanded ATX3 inactivates PNKP phosphatase activity, causing persistent DNA damage and chronic activation of pro apoptotic signaling, which leads to SCA3 pathogenesis."

sparser
"It has been suggested that the PNKP inactivation by mutant ataxin-3 might in part be explained by its recruitment in polyQ aggregates xref ."

reach
"We have shown previously that ATXN3 depleted or pathogenic ATXN3 expressing cells abrogate polynucleotide kinase 3 '-phosphatase (PNKP) activity."
Mutated ATXN3 inhibits PNKP. 7 / 7
| 7

reach
"Conversely, polyQ expanded mutant ATXN3 inhibited PNKP activity, possibly as a result of PNKP sequestration into ATXN3 aggregates."

reach
"In conclusion, our current study provides compelling evidence of how mutant ATXN3 impedes the enzymatic activity of PNKP, and induces DNA damage that manifests with activation of the pro apoptotic signaling pathways in SCA3."

reach
"It has been suggested that the PNKP inactivation by mutant ataxin-3 might in part be explained by its recruitment in polyQ aggregates XREF_BIBR."

reach
"Mutant ATXN3 has also been found to sequester PNKP outside the nucleus and thereby impair its ability to take part in DNA repair [XREF_BIBR]."

reach
"The wild-type ATXN3 protein stimulated, and in contrast, the mutant ATXN3 dramatically diminished, the 3 ' phosphatase activity of PNKP in vitro (Chatterjee et al; Figs."

reach
"Here we report that WT ATXN3 stimulates, and by contrast mutant ATXN3 blocks the DNA 3 '-end-processing activity of PNKP, and the resulting accumulation of DNA SBs may contribute significantly to SCA3 pathogenesis via modulating the DNA damage response pathway."

reach
"These data further support the idea that the mutant ATXN3 specifically blocks the activity of PNKP, but not that of DNA polymerase or ligase (XREF_SUPPLEMENTARY)."
ATXN3 activates PNKP.
| 1 5
ATXN3 activates PNKP. 6 / 10
| 1 5

eidos
"However , how ATXN3 enhances PNKP activity was not clear from these studies ."

reach
"However, how ATXN3 enhances PNKP activity was not clear from these studies."

reach
"Interestingly, it was shown that wild-type ataxin-3 led to stimulation of PNKP, whereas expanded ataxin-3 led to inhibition XREF_BIBR XREF_BIBR."

reach
"Two studies in 2015 showed that ATXN3 binds to and enhances PNKP 3 '-phosphatase activity, promoting DNA repair."

reach
"Our studies described in the accompanying manuscript by Chatterjee et al suggest that PNKP is a native ATXN3 interacting protein, and that ATXN3 modulates PNKP activity and DNA repair (Chatterjee et al, Figs."

reach
"XREF_FIG shows that WT ATXN3 stimulated PNKP 's 3 '-phosphatase activity (ln 2 vs. lns 3-7) ~ 4-fold; in contrast, ATXN3-Q72 reproducibly and significantly abrogated PNKP 's activity (~ 3.5-fold, XREF_FIG, ln 2 vs. ln 6; n = 3)."
| 83

sparser
"Mutations in the Aβ precursor protein gene on chromosome 21, all lying in or near the Aβ peptide region, cause early-onset, autosomal dominant familial forms of AD [ xref – xref ]."

sparser
"In the autosomal dominant forms of AD, the rate of atrophy of medial temporal structures separates affected from control persons even 3 years before the clinical onset of cognitive impairment."

sparser
"Regarding autosomal dominant forms of AD and FTLD, many new genes have been identified, and new diagnostic criteria have been proposed."

sparser
"Most mutations associated with autosomal dominant familial AD (FAD) increase the production or relative abundance of Aβ1-42 ( xref )."

sparser
"Since the discovery of rare early-onset autosomal dominant familial forms of AD caused by missense mutations of the APP gene within the Aβ region, synthetic peptides bearing familial and design mutations have been used to investigate the potential importance of primary sequence in determining Aβ aggregation, toxicity and synaptic disruption [ xref ]."

sparser
"Autosomal dominant forms of AD are due to mutations in the amyloid precursor protein (APP) [ xref , xref ], the protein from which the Aβ peptide present in amyloid plaques is derived, increased copy number for APP, and mutations in Presenilin 1 and 2, whose protein products regulate processing of APP [ xref , xref ]."

sparser
"Mutations in three genes; the amyloid precursor protein (APP), presenilin 1 (PSEN 1), presenilin 2 (PSEN2) have been implicated in the familial early onset (<65years) autosomal dominant form of AD (EOAD) ( xref )."

sparser
"The presence of multiple affected family members in each generation suggested that the disease was an autosomal dominant form of AD."

sparser
"In the autosomal dominant form of AD (ADAD), production of Aβ40 and Aβ42 was 24% higher in mutation carriers than noncarriers, and the fractional turnover rate of Aβ42 was 65% faster in mutation carriers [ xref ]."

sparser
"It is important to note that unlike the mutations in autosomal dominant forms of AD, APOE4 is not a sufficient determinant of AD even in old aged individuals."

sparser
"Familial early-onset AD (FAD) is associated with autosomal dominant mutations in the amyloid precursor protein (APP) and in the catalytic subunits (presenilin 1 and presenilin 2) of the intramembrane protease that processes it, γ-secretase ( xref )."

sparser
"Early-onset autosomal dominant AD genes are associated with excessive accumulation, however cognitive impairment best correlates with NFTs and disrupted microtubules."
STUB1 affects ATXN3
7 1 1 | 1 45 27
STUB1 binds ATXN3.
7 | 20 21
7 | 20 21

reach
"To test this we performed pull-down assays to assess ataxin-3 binding to Ub-CHIP in the presence of mono-, di-, tri-, or hexa-K63 ubiquitin chains, a preferred substrate for ataxin-3."

sparser
"To determine whether CHIP interacts with full length ataxin-3 we performed co-IPs with CHIP and ataxin-3 in lysates from transfected cells."

reach
"Intriguingly, monoubiquitination of CHIP appears to enhance the interaction between ataxin-3 and CHIP, and when bound, ataxin-3 can now deubiquitinate CHIP."

sparser
"Thus, CHIP and ataxin-3 interact and regulate the activity of each other."

sparser
"The mammalian protein CHIP is a U-box domain-containing cytosolic PQC E3 ligase that facilitates the degradation of misfolded proteins with the help of chaperones HSP70 or HSP90. xref In the heart, CHIP has a strong cardioprotective effect, as demonstrated by inhibition of apoptosis following I/R injury, xref angiotensin II-induced cardiac remodeling, xref and myocardial infarction. xref It was recently found that monoubiquitination of CHIP by an E2 enzyme Ube2w promotes the interaction of CHIP with Ataxin-3, a deubiquitinating enzyme (DUB)."

sparser
"Though ataxin-3 does interact with unmodified CHIP, it binds with ~18 fold increase in affinity to Ub-CHIP ( xref )."

sparser
"Monoubiquitination of CHIP by Ube2w stabilizes the interaction between CHIP and ataxin-3, which through its DUB activity limits the length of chains attached to CHIP substrates."

sparser
"Monoubiquitination of CHIP by Ube2w promotes the interaction of CHIP with ATXN3, which in turn through its DUB activity restricts the length of ubiquitin chains attached to CHIP substrates."

reach
"Important insights into the functional interaction between ATXN3 and CHIP have recently been elucidated."

reach
"Ataxin-3 presumably binds polyubiquitinated substrates through its ubiquitin interacting domain and deubiquitinates CHIP to terminate the reaction."
STUB1 ubiquitinates ATXN3.
1 | 9 6
STUB1 ubiquitinates ATXN3. 10 / 16
1 | 9 6

reach
"E3 ubiquitin ligase CHIP along with Hsp70 ubiquitinates and degrades polyglutamine aggregates of mutant huntingtin and ataxin-3, as depicted in Figure XREF_FIG."

reach
"Without HSP70, CHIP can ubiquitinate Atx3 in vitro, but with a rather low efficiency."

sparser
"However, recent work indicated that CHIP ubiquitinates ataxin-3 to modify its catalytic activity during the process of substrate ubiquitination, where ataxin-3 and CHIP cooperate to enhance CHIP substrate degradation xref , xref , xref ."

sparser
"Without HSP70, CHIP can ubiquitinate Atx3 in vitro , but with a rather low efficiency."

reach
"However, recent work indicated that CHIP ubiquitinates ataxin-3 to modify its catalytic activity during the process of substrate ubiquitination, where ataxin-3 and CHIP cooperate to enhance CHIP substrate degradation XREF_BIBR, XREF_BIBR, XREF_BIBR."

reach
"Whether CHIP is the primary mediator of ubiquitin dependent degradation of ataxin-3 is controversial : XREF_BIBR reported that CHIP ubiquitinates ataxin-3 and directs it to the proteasome for degradation, whereas XREF_BIBR reported that another ubiquitin ligase, E4B, mediates ataxin-3 degradation while CHIP has no effect."

sparser
"According to a previous report, the E3 ubiquitin ligase CHIP may ubiquitinate ataxin-3 as well as other polyQ proteins to target them for degradation xref ."

reach
"Surprisingly, CHIP over-expression readily increases the ubiquitination of both wild type and polyQ ATXN1 and ATXN3 [XREF_BIBR, XREF_BIBR, XREF_BIBR]."

reach
"Conversely, CHIP ubiquitinates wild-type ataxin-3, which in turn enhances the overall deubiquitinating activity of ataxin-3."
STUB1 activates ATXN3.
| 8
STUB1 activates ATXN3. 8 / 8
| 8

reach
"It was recently shown that CHIP promotes the degradation of polyQ expanded ataxin-3 and, together with Hsc70 protein, suppresses the aggregation and cytotoxicity mediated by huntingtin (Jana et al., 2[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Although in this case, the E3 ligases that are acting redundantly to CHIP have not been identified, overexpression of either CHIP or parkin promotes the degradation of nNOS and polyglutamine expanded ataxin-3."

reach
"However, further analysis revealed certain discrepancies in the CHIP 's effects on different polyQ proteins; for example, CHIP increases the degradation of polyQ expanded ataxin-3 while it does not af[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Transient overexpression of CHIP increases the ubiquitination and the rate of degradation of polyglutamine expanded huntingtin or ataxin-3."

reach
"It was previously reported that overexpression of CHIP increases the ubiquitination and degradation rates of polyQ expanded Atx3, which is also enhanced by HSP70 XREF_BIBR."

reach
"The possibility of redundancy in E3 ligase action is suggested by reports that overexpression of either CHIP or Parkin increases ubiquitination of polyglutamine expanded ataxin-3 and reduces its cellular toxicity."

reach
"XREF_FIG - XREF_FIG demonstrate that CHIP promotes degradation of two signaling proteins, GR and nNOS, and two poly Q expanded proteins, AR112Q and Q78 ataxin-3."

reach
"Our findings and recent reports lead us to favor a model in which mono-ubiquitination of CHIP promotes recruitment of ataxin-3 to the complex before chain formation on substrates."
STUB1 inhibits ATXN3.
| 1 6
STUB1 inhibits ATXN3. 7 / 7
| 1 6

reach
"Additionally, reduction in CHIP expression enhances expanded ataxin-3 toxicity in vivo."

reach
"In contrast to ataxin-1, CHIP indeed promoted the degradation of polyQ expanded ataxin-3 (73Q) without causing its accumulation in the insoluble fraction, especially at the highest concentration."

reach
"The dose dependent phenotype caused by CHIP reduction in Q71-B mice allowed us to determine whether CHIP reduction enhances ataxin-3 aggregation and to correlate biochemically detectable changes in aggregation to behavioral phenotype."

eidos
"CHIP has also been shown to degrade Ataxin-3 [ 59 ] ."

reach
"CHIP has also been shown to degrade Ataxin-3 [XREF_BIBR]."

reach
"Interestingly, when expanded ataxin-3 was present, CHIP levels were also reduced in the brains of MJD transgenic mice, raising the possibility that loss of one or both E3 partners may be a contributing factor in the pathogenesis of SCA3."

reach
"In parallel, CHIP reduction markedly increases the level of ataxin-3 microaggregates, which partition in the soluble fraction of brain lysates yet are resistant to dissociation with denaturing detergent, and which precede the appearance of inclusions."
STUB1 decreases the amount of ATXN3.
1 | 2
STUB1 decreases the amount of ATXN3. 3 / 3
1 | 2

"For example, overexpression of the human TPR domain-containing co- chaperone CHIP suppresses neurodegeneration in fly models expressing polyQ-containing versions of ataxin 1 and the N-terminal huntingtin fragment (Al-Ramahi et al., 2006)."

reach
"As a result, silencing of CHIP significantly increases the amount of Atx3 (XREF_FIG), suggesting that CHIP may down-regulate the Atx3 level."

reach
"Also, consistent with effects in non neuronal cells, both CHIP and Parkin decreased Q78 ataxin-3 levels in MN-1 neuronal cells but Mdm2 did not (XREF_FIG)."
ATXN3 affects TP53
5 1 | 36 19
ATXN3 binds TP53.
5 | 11 17
5 | 11 17

sparser
"Li-Fraumeni syndrome (LFS) is an autosomal dominant disease that is associated with germline TP53 mutations and it predisposes affected individuals to a high risk of developing multiple tumors."

reach
"We wonder whether the polyQ expansion affects the ATX-3 and p53 interaction."

reach
"We have found that ATX-3 interacts with p53 and functions as a novel p53 DUB."

sparser
"Therefore, both the Josephin and UIM domain coordinately regulate the interaction between ATX-3 and p53 ( xref )."

sparser
"The interaction between ATX-3 and p53 was confirmed under physiological condition ( xref ) and with in vitro purified forms ( xref ), indicating a direct association between these two proteins."

No evidence text available

sparser
"Consistently, as shown in xref , the cysteine 14 mutation did not affect the binding of ATX-3 to either native or ubiquitinated p53, whereas the ΔN mutant lost its binding to both forms of p53."

sparser
"Instructively, mutations effecting the expression of p53 are associated with Li Fraumini syndrome, an autosomal dominant hereditary cancer predisoposition syndrome."

sparser
"As ATX-3 interacts with p53 under physiological conditions and regulates the ubiquitination of p53 in cells, it is possible that ATX-3 may regulate the turnover of p53 via the Ub-proteasome pathway."

No evidence text available
ATXN3 activates TP53.
| 16 2
ATXN3 activates TP53. 10 / 18
| 10 2

reach
"When the FL ATX-3 mRNA was injected into WT but not the p53 mutant zebrafish embryos, significantly more apoptotic cells were observed in TUNEL staining assays, indicating that ATX-3 caused p53 dependent apoptosis in vivo."

reach
"Using the zebrafish model system, we further examined whether ATX-3 induces p53 dependent apoptosis in vivo."

sparser
"Furthermore, to test whether mutant ATXN3 activates p53 and Chk2 via activating ATM, we pre-treated the cells with ATM inhibitor Ku55933 and expressed ATXN3-Q84 and assessed the activation of DNA damage response pathway."

reach
"Flow cytometry analysis using Annexin V-FITC and propidium iodide (PI) staining in HCT116 cells showed that knockdown of ATX-3 led to a decrease of camptothecin (CPT)-induced apoptosis, while ectopic expression of ATX-3 but not the catalytic inactive mutant ATX-3-C14A resulted in a significant increase of apoptosis in HCT116 p53 +/+ but not HCT116 p53 -/- cells (XREF_FIG), indicating that ATX-3 promoted p53 mediated apoptosis, which required its deubiquitinating enzymatic activity."

reach
"As P53 has known functions in cycle arrest and apoptosis, this indicates that expression of atxn3 polyQ repeats induces selective transcription and expression of p53 target genes and promotes p53 dependent apoptosis in the CNS of zebrafish [XREF_BIBR]."
| PMC

reach
"ATX-3 Promotes p53 Dependent Apoptosis in Cells and in Zebrafish."

reach
"Deletion of ATXN3 resulted in destabilization of p53, whereas ectopic expression of ATXN3 induced expression of p53 target genes and promoted p53-dependent apoptosis."

reach
"The expression levels of p53 responsive genes (such as p21 and Puma) were also higher in ATX-3 exp (80Q) expressing RKO cells (XREF_SUPPLEMENTARY), suggesting that p53 was functionally enhanced by polyQ expansion in ATX-3."

sparser
"Activation of p53 by mutant ataxin-3 is also related to phosphorylation of p53 at ser15 residue in the SCA3 transgenic mice model."

reach
"Besides, we found that the degradation of p53 in the ATX-3 exp (80Q)-expressing cells was slower than that of normal ATX-3-expressing cells (XREF_SUPPLEMENTARY), and ectopic expression of polyQ expanded ATX-3 induced higher levels of p53 protein than the normal ATX-3 in RKO, 293T, and MEF cells (XREF_SUPPLEMENTARY), indicating that polyQ expanded ATX-3 possessed enhanced capability to stabilize p53."
Mutated ATXN3 activates TP53. 6 / 6
| 6

reach
"Mutant ATXN3 activates the pro apoptotic p53 pathway by activating ATM in SCA3."

reach
"Specific modulation of mutant ATXN3 mediated atypical activation of the DNA damage response p53 and PKCdelta pathways, or enhancing the efficacy of in vivo DNA damage repair may be effective strategies to combat the pathways leading to systemic neurodegeneration in SCA3."

reach
"Knockdown of normal ATXN3 or PNKP, or expression of mutant ATXN3 increased the accumulation of DNA damage and persistent activation of the ATM and p53 dependent DNA repair pathway, suggesting ATXN3 may be a key regulator of PNKP dependent DNA repair."

reach
"Notably, compared with normal ataxin-3 protein, the gain-of-function mutant ataxin-3 triggers higher p53 protein and p53 responsive gene expression and causes more severe p53 dependent neurodegeneration in brain neuron cells of transgenic zebrafish and SCA3 mice 13."

reach
"Furthermore, to test whether mutant ATXN3 activates p53 and Chk2 via activating ATM, we pre-treated the cells with ATM inhibitor Ku55933 and expressed ATXN3-Q84 and assessed the activation of DNA damage response pathway."

reach
"Consistent with our hypothesis, ATXN3-Q84 expression failed to stimulate phosphorylation of Chk2 and p53 in the presence of the ATM inhibitor Ku55933 (XREF_SUPPLEMENTARY), substantiating our interpretation that mutant ATXN3 stimulates the DNA damage response p53 pathway via activating ATM."
ATXN3 phosphorylates TP53.
| 4
Mutated ATXN3 phosphorylates TP53. 4 / 4
| 4

reach
"Not only in primary cultures of central nervous cells expressing mutant ataxin-3 but also in pontine nuclei of SCA3 transgenic mice, mutant ataxin-3 can increase the expression of total and phosphorylated p53 as well as the transcriptional activity of p53, which is associated with upregulation of Bax and activated caspase-3 and 9 expression and subsequent apoptotic cell death XREF_BIBR, XREF_BIBR."

reach
"Moreover, mutant ataxin-3 increases the phosphorylation of p53 and enhances the transcriptional activity of p53, which in turn amplifies pro apoptotic protein Bax expression in cultured cerebellar and pontine nuclei neurons XREF_BIBR, XREF_BIBR."

reach
"However, the mechanism by which mutant ATXN3 increases p53 phosphorylation and activates the p53 dependent pro apoptotic signaling pathways to facilitate neuronal death and dysfunction remains unknown."

reach
"For example, the HAT CBP also acts as a CREB binding protein and can take part in transcriptional dysfunction in HD, whereas post-translational modification affects transcriptional regulation, mutant ataxin-3 and mutant ataxin-7 could cause phosphorylation and ubiquitination of p53, resulting in p53 transcriptional regulation."
ATXN3 inhibits TP53.
| 1
ATXN3 inhibits TP53. 1 / 3
| 1

reach
"ATX-3 deletion destabilizes p53, resulting in deficiency of p53 activity and functions, whereas ectopic expression of ATX-3 induces selective transcription and expression of p53 target genes and promotes p53 dependent apoptosis in both mammalian cells and the central nervous system of zebrafish."
ATXN3 deubiquitinates TP53.
1 | 2
ATXN3 deubiquitinates TP53. 3 / 3
1 | 2

reach
"To determine whether ATX-3 can deubiquitinate p53 directly in vitro, we performed in vitro deubiquitination assays."

reach
"Thus, ATX-3 deubiquitinates and stabilizes p53 (XREF_FIG), which is an essential step for p53 function in cell cycle arrest and apoptosis (XREF_FIG)."
ATXN3 increases the amount of TP53.
| 2
ATXN3 increases the amount of TP53. 2 / 2
| 2

reach
"One can hypothesize that SCA3 and other neurodegenerative disorders involve a positive feedback between proteasomal degradation, aggregation, GSK3 activation, and increased p53 levels."

reach
"Deletion of ATXN3 resulted in destabilization of p53, whereas ectopic expression of ATXN3 induced expression of p53 target genes and promoted p53-dependent apoptosis."
ATXN3 affects STUB1
7 1 | 41 21
ATXN3 binds STUB1.
7 | 20 21
7 | 20 21

reach
"To test this we performed pull-down assays to assess ataxin-3 binding to Ub-CHIP in the presence of mono-, di-, tri-, or hexa-K63 ubiquitin chains, a preferred substrate for ataxin-3."

sparser
"To determine whether CHIP interacts with full length ataxin-3 we performed co-IPs with CHIP and ataxin-3 in lysates from transfected cells."

reach
"Intriguingly, monoubiquitination of CHIP appears to enhance the interaction between ataxin-3 and CHIP, and when bound, ataxin-3 can now deubiquitinate CHIP."

sparser
"Thus, CHIP and ataxin-3 interact and regulate the activity of each other."

sparser
"The mammalian protein CHIP is a U-box domain-containing cytosolic PQC E3 ligase that facilitates the degradation of misfolded proteins with the help of chaperones HSP70 or HSP90. xref In the heart, CHIP has a strong cardioprotective effect, as demonstrated by inhibition of apoptosis following I/R injury, xref angiotensin II-induced cardiac remodeling, xref and myocardial infarction. xref It was recently found that monoubiquitination of CHIP by an E2 enzyme Ube2w promotes the interaction of CHIP with Ataxin-3, a deubiquitinating enzyme (DUB)."

sparser
"Though ataxin-3 does interact with unmodified CHIP, it binds with ~18 fold increase in affinity to Ub-CHIP ( xref )."

sparser
"Monoubiquitination of CHIP by Ube2w stabilizes the interaction between CHIP and ataxin-3, which through its DUB activity limits the length of chains attached to CHIP substrates."

sparser
"Monoubiquitination of CHIP by Ube2w promotes the interaction of CHIP with ATXN3, which in turn through its DUB activity restricts the length of ubiquitin chains attached to CHIP substrates."

reach
"Important insights into the functional interaction between ATXN3 and CHIP have recently been elucidated."

reach
"Ataxin-3 presumably binds polyubiquitinated substrates through its ubiquitin interacting domain and deubiquitinates CHIP to terminate the reaction."
ATXN3 deubiquitinates STUB1.
1 | 19
ATXN3 deubiquitinates STUB1. 10 / 20
1 | 19

"Indeed, ataxin-3 not only deubiquitinated CHIP, but also trimmed Ub conjugates on CHIP substrates, thereby regulating the length of Ub chains."

reach
"Upon completion of substrate ubiquitination, as determined by chain length, ataxin-3 presumably binds ubiquitinated substrate through its UIMs and deubiquitinates CHIP, thus terminating the reaction (XREF_FIG) [XREF_BIBR]."

reach
"Importantly, ataxin-3 deubiquitinates CHIP, terminating CHIP-substrate interaction; polyQ expansion of ataxin-3 increases its affinity for CHIP and decreases CHIP levels in SCA3 mice, suggesting a surprising role for coordinated regulation of CHIP and ataxin-3 as well as dysregulation of this process in SCA3."

reach
"Evidence further supporting this notion that the ability of ataxin-3 to deubiquitinate CHIP is coupled to the ligase activity of CHIP, came from assays in which ataxin-3-mediated deubiquitination of CHIP did not occur until after poly-Ub conjugates on substrate proteins had attained a certain length."

reach
"The above results do not exclude the possibility that ubiquitin chains per se rather than polyubiquitinated substrates facilitate deubiquitination of CHIP by ataxin-3."

reach
"To determine whether deubiquitination of Ub-CHIP by ataxin-3 requires the presence of ubiquitinated substrate, we performed CHIP ubiquitination reactions with increasing concentrations of HSP90 as substrate, in the presence or absence of ataxin-3 and the chain elongating E2, UbcH5c (XREF_FIG), then assessed the ubiquitin state of CHIP at the end of the reaction."

reach
"Once this occurred, ataxin-3 could now deubiquitinate both CHIP and its substrates, trimming the Ub conjugates at the distal ends."

reach
"Ataxin-3 deubiquitinates CHIP upon completion of substrate polyubiquitination."

reach
"In response to substrate polyubiquitination, ataxin-3 deubiquitinates CHIP."

reach
"In reactions containing ataxin-3 and Ube2w but not UbcH5c (thus preventing chain extension), ataxin-3 did not deubiquitinate Ub-CHIP (XREF_FIG)."
ATXN3 decreases the amount of STUB1.
| 2
ATXN3 decreases the amount of STUB1. 2 / 2
| 2

reach
"Importantly, ataxin-3 deubiquitinates CHIP, terminating CHIP-substrate interaction; polyQ expansion of ataxin-3 increases its affinity for CHIP and decreases CHIP levels in SCA3 mice, suggesting a surprising role for coordinated regulation of CHIP and ataxin-3 as well as dysregulation of this process in SCA3."

reach
"Moreover, ataxin-3 also induces a reduction in CHIP levels."
ATXN3 affects Ubiquitin
| 20 24
ATXN3 binds Ubiquitin.
| 2 24
| 2 20

reach
"The second critical feature are its three Ub interacting motifs (UIMs), through which ataxin-3 binds Ub conjugates and ubiquitinated proteins, bringing it into proximity to trim or edit specific linkages within these Ub conjugates."

sparser
"The disease protein ataxin-3 has been associated with the ubiquitin-proteasome system and transcriptional regulation."

sparser
"The energy landscapes derived from the best fitting ensembles of free and ubiquitin bound ataxin-3 showed an increased conformational entropy upon binding ubiquitin, consistent with previous NMR relaxation data xref ."

sparser
"Ataxin-3 presumably binds polyubiquitinated substrates through its ubiquitin-interacting domain and deubiquitinates CHIP to terminate the reaction ( xref )."

sparser
"We show that wild-type Atx-3 is a Ub-binding protein and that the interaction of Atx-3 with Ub is mediated by motifs homologous to those found in a proteasome subunit."

sparser
"Two major full-length isoforms of ataxin-3 exist, both of which contain the same N-terminal portion and polyQ repeat, but differ in their C-termini; one (denoted here as isoform 1) contains a motif that binds ataxin-3’s substrate, ubiquitin, whereas the other (denoted here as isoform 2) has a hydrophobic tail."

sparser
"Hsp104 rescued forms of MJD unable to engage polyubiquitin or with a deletion in the deubiquitylase domain indicating that MJD-ubiquitin interactions hinder protective Hsp104 activities."

sparser
"Still, we wondered whether proteins other than ubiquitin also interact with ataxin-3 through UIMs."

sparser
"Hsp104 suppressed toxicity of MJD variants lacking a portion of the N-terminal deubiquitylase domain and full-length MJD variants unable to engage polyubiquitin, indicating that MJD-ubiquitin interactions hinder protective Hsp104 modalities."

sparser
"To determine catalytic residues that might facilitate the selective threonine esterase activity, we studied the experimental ATXN3-Ub structure and its superposition with our JOSD1 homology model ( xref )."
| 4

sparser
"Furthermore, the ATXN3 C14A mutant co-IPed more Ub-Rheb than wild-type ATXN3 ( xref ), suggesting that the binding of inactive ATXN3 to the Ub-Rheb may prevent its deubiquitination in the cells or during the purification of the ATXN3-Rheb complex."

sparser
"As expected, the four lysine residues of Rheb, which are responsible for the polyubiquitination of Rheb, are important for the binding of Ub-Rheb with ATXN3 ( xref )."

sparser
"In fact, higher levels of Ub-Rheb were co-IPed with ATXN3 when N-Ethylmaleimide, a deubiquitinase inhibitor, was included in the lysis and purification buffers ( xref ), supporting the idea that the Ub-Rheb that binds to ATXN3 was subjected to deubiquitination during the isolation processes."

sparser
"These observations support the idea that ATXN3 translocates to the lysosome and interacts with Ub-Rheb for its deubiquitination in a manner dependent on the inactive Rag heterodimer in response to amino acid starvation."
ATXN3 inhibits Ubiquitin.
| 11
| 11

reach
"Together, these results indicate that ataxin-3 functions with CHIP to prevent the incorporation of additional ubiquitin to chains on substrates once they reach a certain length."

reach
"Thus, in one possible mechanism of suppression, ataxin-3 could suppress toxicity by simply increasing the pool of free ubiquitin."

reach
"For instance, the deubiquitinase Ataxin-3 was shown to interact with C-terminus of Hsc70 Interacting Protein (CHIP), a major mammalian E3 ligase involved in cytosolic PQC, to limit the length of ubiquitin chains built on CHIP substrates XREF_BIBR."

reach
"By binding the minimum length poly-ubiquitin chain on substrates, ATXN3 may prevent complete removal of the ubiquitin chain by other DUBs, and thus facilitate its recognition by the 26S proteasome."

reach
"We have found that aggregation of polyQ expanded Atx3 can sequester P97 and Ub conjugates into the protein aggregates or inclusions through specific interactions both in vitro and in cells."

reach
"These results support a model in which ataxin-3 effectively limits ubiquitin chain extension once chains reach a critical length."

reach
"When substrates are soluble, ATX3 prevents their premature degradation by removing K48‐linked Ub chains to slow down the rate of p97‐dependent degradation."

reach
"PolyQ expanded huntingtin and ataxin-3 sequester ubiquitin adaptors hHR23B and UBQLN2 into aggregates via conjugated ubiquitin."

reach
"Absence of ataxin-3 UIMs 1 and 2 shows reduced binding of ubiquitin chains."

reach
"An unbiased, semi-automated quantitative analysis (XREF_SUPPLEMENTARY) revealed that knock-down of ataxin-3 indeed impaired the accumulation ofRNF8 (Fig XREF_FIG A), RNF168 (Fig XREF_FIG B), and ubiquitin conjugates atlaser induced DSBs (Fig XREF_FIG C)."
ATXN3 activates Ubiquitin.
| 7
| 7

reach
"Whether ataxin 3 produces unanchored ubiquitin chains and activates HDAC6 remains to be tested."

reach
"However, inactivation of ATX3 only increased the accumulation of K63‐Ub at sites of DNA damage."

reach
"As reported previously, ataxin-3 (Q22) deubiquitinates K63 linked ubiquitin chains and promotes the appearance of smaller ubiquitin chains over time."

reach
"Through this process, ataxin-3 can facilitate substrate entry into the proteasome, as well as mediate other ubiquitin dependent pathways."

reach
"CHIP associated Ataxin-3 promotes the efficacy of Ub labeling on CHIP substrates, probably by preventing the lengthy Ub chain extension on CHIP substrates."

reach
"Mono-ubiquitination of ataxin-3, which is enhanced by proteotoxic stress, increases its ubiquitin hydrolase activity XREF_BIBR."

reach
"Ataxin-3 containing the 71Q expansion associated with RNF8 mediated ubiquitin chains with similar efficiency as wild-type ataxin-3 (10Q), indicating that the expanded polyQ stretch does not affect the ubiquitin binding activity of ataxin-3."
PNKP affects ATXN3
2 | 22 32
2 | 10 32

sparser
"Our data in the accompanying manuscript by Chatterjee et al suggest that mutant ATXN3 binds PNKP and inhibits its 3’-phosphatase activity (Chatterjee et al, Figs. xref and xref )."

sparser
"We have made the unexpected observation that PNKP stably associates with Ataxin-3 (ATXN3), a polyglutamine repeat-containing protein mutated in spinocerebellar ataxia type 3 (SCA3), also known as Machado-Joseph Disease (MJD)."

sparser
"Together, these data support our previous interpretation that both wild-type and mutant ATXN3 interact with PNKP in the cell (Chatterjee et al)."

sparser
"The PLA analysis showed no significant interaction of ATXN3 with DNA LIG 3α in the brain sections from SCA3 patients, or in control brains under identical experimental conditions ( xref ; panels 1 and 4), suggesting specificity of the interactions between ATXN3 and PNKP in vivo ."

sparser
"Two studies in 2015 showed that ATXN3 binds to and enhances PNKP 3’-phosphatase activity, promoting DNA repair ( xref ; xref )."

reach
"To further confirm the interaction of PNKP and ATXN3 in cell, we performed bi-molecular fluorescence complementation (Bi-FC) assays, a versatile method to assess in cell protein protein interactions [XREF_BIBR]."

sparser
"To examine ATXN3’s association with PNKP, we immunoprecipitated (IP’d) PNKP and ATXN3 separately from the nuclear extract (NE, benzonase treated to remove DNA and RNA to avoid DNA-mediated co-immunoprecipitation) of human HEK-293 (human embryonic kidney cell line) ( xref ) and SH-SY5Y (a human neuroblastoma cell line) cells ( xref ) using the respective anti-protein (PNKP or ATXN3) antibody (Ab)."

sparser
"Although Chatterjee et al. found that polyQ-ataxin-3 does not interact with the phosphatase domain of PNKP in vitro, could mutant ataxin-3 nonetheless interact with PNKP in its native complex to inactivate its phosphatase activity?"

sparser
"Interestingly, the PNKPATXN3 interaction predominantly occurs outside of the nucleus in SCA3 patient brains, and the authors document colocalization of PNKP with polyQ-containing aggregates in SCA3 patient brains."

sparser
"We report that human polynucleotide kinase 3’-phosphatase (PNKP), a major DNA strand break repair enzyme, stably associates with Ataxin-3 (ATXN3)."
Mutated ATXN3 binds PNKP. 10 / 12
| 12

reach
"In the present study, we show that the essential DNA strand break repair enzyme PNKP (polynucleotide kinase 3 '-phosphatase) interacts with, and is inactivated by, the mutant ATXN3, resulting in inefficient DNA repair, persistent accumulation of DNA damage and strand breaks, and subsequent chronic activation of the DNA damage response ataxia telangiectasia mutated (ATM) signaling pathway in SCA3."

reach
"Here we have clearly shown that both WT and mutant ATXN3 interact directly with PNKP."

reach
"Studies of other polyQ diseases suggest that interactions in the soluble phase are an important aspect of the pathogenic cascade; hence, it is also possible that mutant ataxin-3 deleteriously interacts with PNKP independent of aggregate sequestration (XREF_FIG)."

reach
"These data substantiate our interpretation that both wild-type and mutant ATXN3 interact with PNKP in the cell."

reach
"Together, these data support our previous interpretation that both wild-type and mutant ATXN3 interact with PNKP in the cell (Chatterjee et al)."

reach
"Further, GST pull-down from the nuclear extract, followed by Western blot analysis, indicated that both wild-type and mutant ATXN3 directly interact with PNKP in vitro, (Chatterjee et al; XREF_FIG)."

reach
"In contrast, mutant ATXN3 interacts with PNKP and abrogates its 3 '-phosphatase activity, resulting in increased accumulation of DNA damage that chronically activates ATM --> p53 and ATM --> c-Abl --> PKCdelta pro apoptotic signaling to trigger neuronal dysfunction and apoptosis in SCA3 (illustrated in XREF_FIG)."

reach
"Although Chatterjee et al. found that polyQ-ataxin-3 does not interact with the phosphatase domain of PNKP in vitro, could mutant ataxin-3 nonetheless interact with PNKP in its native complex to inactivate its phosphatase activity?"

reach
"Our data in the accompanying manuscript by Chatterjee et al suggest that mutant ATXN3 binds PNKP and inhibits its 3 '-phosphatase activity (Chatterjee et al, Figs."

reach
"PNKP interacts with the mutant ATXN3 and is sequestered into the ATXN3-polyQ aggregates in SCA3 brain."
ATXN3 affects CHEK1
1 | 30 9
ATXN3 binds CHEK1.
| 14 9
| 14 9

reach
"In contrast, treatment with ETO, which has little effect on Chk1 expression, did not change the interaction between ATX3 and Chk1 significantly (XREF_SUPPLEMENTARY)."

reach
"In support of our idea that S345 phosphorylation is involved in regulating the interaction between ATX3 and Chk1 after persistent replication stress, the decreased association between ATX3 and Chk1 under replicative stress was restrained when Chk1 S345 phosphorylation was inhibited by CGK733, an inhibitor of ATM and ATR."

reach
"We have demonstrated the ATX3 and Chk1 interaction under unperturbed conditions."

reach
"Under unperturbed conditions and upon DNA damage, ataxin-3 (ATX3) interacts with Chk1 and protects it from DDB1 and CUL4A- and FBXO6 and CUL1 mediated polyubiquitination and subsequent degradation, thereby promoting DNA repair and checkpoint signaling."

reach
"We wondered whether polyQ expansion compromises the interaction between ATX3 and Chk1."

sparser
"In contrast, treatment with ETO, which has little effect on Chk1 expression, did not change the interaction between ATX3 and Chk1 significantly ( xref )."

reach
"Ser345 phosphorylation which targets Chk1 for polyubiquitination and degradation plays an essential role in regulating the interaction between Chk1 and ATX3."

sparser
"Under unperturbed conditions and upon DNA damage, ataxin-3 (ATX3) interacts with Chk1 and protects it from DDB1/CUL4A- and FBXO6/CUL1-mediated polyubiquitination and subsequent degradation, thereby promoting DNA repair and checkpoint signaling."

reach
"Given that S345 phosphorylation of Chk1, mediated by ATR, targets Chk1 for degradation via the ubiquitin-proteasome pathway, we next assessed the impact of mutation of this phosphorylation site on the ATX3 and Chk1 interaction."

sparser
"To further define the interaction between ATX3 and Chk1, we generated a series of truncations of ATX3 and Chk1, respectively."
ATXN3 activates CHEK1.
| 6
ATXN3 activates CHEK1. 6 / 14
| 6

reach
"Indeed, overexpression of FBXO6 or DDB1 resulted in significant decrease of Chk1 protein level, whereas co-expression of ATX3 effectively restored Chk1 from E3 ligase mediated degradation."

reach
"Moreover, ATX3 depleted cells re-entered the cell cycle more rapidly than WT cells did, implying that Chk1 reduction caused by ATX3 knockout can accelerate recovery from the G2/M damage checkpoint, which is consistent with previous results from Chk1-/- ES cells."

reach
"To test if ATX3 may promote Chk1 stabilization, we first evaluated whether ATX3 deficiency impairs Chk1 stability."

reach
"Ataxin-3 promotes genome integrity by stabilizing Chk1."

reach
"Taken together, our results demonstrated that ATX3 promoted Chk1 stability through the ubiquitin-proteasome pathway."

reach
"As mentioned above, ATX3 promotes Chk1 stability through inhibiting its proteasomal destruction."
ATXN3 inhibits CHEK1.
| 3
ATXN3 inhibits CHEK1. 3 / 7
| 3

reach
"Expression of FBXO6 induced higher level of polyubiquitination in Chk1, which can be remarkably decreased by co-expression of ATX3."

reach
"ATX3 deficiency impairs Chk1 mediated G2/M DNA damage checkpoint."

reach
"To test if ATX3 may promote Chk1 stabilization, we first evaluated whether ATX3 deficiency impairs Chk1 stability."
ATXN3 increases the amount of CHEK1.
| 4
Modified ATXN3 increases the amount of CHEK1. 4 / 4
| 4

reach
"In subsequent experiments, we investigated whether overexpression of ATX3 upregulates the steady-state Chk1 level."

reach
"Indeed, ectopic expression of Flag-ATX3 in A549 cells nicely upregulated the endogenous level of Chk1, and promoted the stability of Chk1 after CHX treatment."

reach
"If ATX3 stabilizes Chk1, then forced expression of ATX3 should upregulate the steady-state level and stability of Chk1."

reach
"Indeed, transient lentiviral expressions of ATX3 in WT and KO cells caused higher levels of Chk1 than control cells transfected with vector."
ATXN3 deubiquitinates CHEK1.
1 | 3
ATXN3 deubiquitinates CHEK1. 4 / 4
1 | 3

reach
"Our result indicated that GST-ATX3 effectively decreased the polyubiquitination of Chk1 in a dose dependent manner."

"Taken together, these findings reveal ATX3 to be a novel deubiquitinase of Chk1, providing a new mechanism of Chk1 stabilization in genome integrity maintenance."

reach
"We discover that, under unperturbed conditions and upon DNA damage, deubiquitination of Chk1 by ATX3 limits its polyubiquitination and subsequent degradation mediated by CUL1- and CUL4A- containing E3 ligase complexes, thus promoting Chk1 stabilization and further checkpoint signaling and DNA repair."

reach
"Therefore, it is plausible that ATX3, as a DUB, functions to deubiquitinate Chk1 to counteract the action of E3 ligases."
Ubiquitin affects ATXN3
| 8 25
Ubiquitin binds ATXN3.
| 2 24
| 2 20

reach
"The second critical feature are its three Ub interacting motifs (UIMs), through which ataxin-3 binds Ub conjugates and ubiquitinated proteins, bringing it into proximity to trim or edit specific linkages within these Ub conjugates."

sparser
"The disease protein ataxin-3 has been associated with the ubiquitin-proteasome system and transcriptional regulation."

sparser
"The energy landscapes derived from the best fitting ensembles of free and ubiquitin bound ataxin-3 showed an increased conformational entropy upon binding ubiquitin, consistent with previous NMR relaxation data xref ."

sparser
"Ataxin-3 presumably binds polyubiquitinated substrates through its ubiquitin-interacting domain and deubiquitinates CHIP to terminate the reaction ( xref )."

sparser
"We show that wild-type Atx-3 is a Ub-binding protein and that the interaction of Atx-3 with Ub is mediated by motifs homologous to those found in a proteasome subunit."

sparser
"Two major full-length isoforms of ataxin-3 exist, both of which contain the same N-terminal portion and polyQ repeat, but differ in their C-termini; one (denoted here as isoform 1) contains a motif that binds ataxin-3’s substrate, ubiquitin, whereas the other (denoted here as isoform 2) has a hydrophobic tail."

sparser
"Hsp104 rescued forms of MJD unable to engage polyubiquitin or with a deletion in the deubiquitylase domain indicating that MJD-ubiquitin interactions hinder protective Hsp104 activities."

sparser
"Still, we wondered whether proteins other than ubiquitin also interact with ataxin-3 through UIMs."

sparser
"Hsp104 suppressed toxicity of MJD variants lacking a portion of the N-terminal deubiquitylase domain and full-length MJD variants unable to engage polyubiquitin, indicating that MJD-ubiquitin interactions hinder protective Hsp104 modalities."

sparser
"To determine catalytic residues that might facilitate the selective threonine esterase activity, we studied the experimental ATXN3-Ub structure and its superposition with our JOSD1 homology model ( xref )."
| 4

sparser
"Furthermore, the ATXN3 C14A mutant co-IPed more Ub-Rheb than wild-type ATXN3 ( xref ), suggesting that the binding of inactive ATXN3 to the Ub-Rheb may prevent its deubiquitination in the cells or during the purification of the ATXN3-Rheb complex."

sparser
"As expected, the four lysine residues of Rheb, which are responsible for the polyubiquitination of Rheb, are important for the binding of Ub-Rheb with ATXN3 ( xref )."

sparser
"In fact, higher levels of Ub-Rheb were co-IPed with ATXN3 when N-Ethylmaleimide, a deubiquitinase inhibitor, was included in the lysis and purification buffers ( xref ), supporting the idea that the Ub-Rheb that binds to ATXN3 was subjected to deubiquitination during the isolation processes."

sparser
"These observations support the idea that ATXN3 translocates to the lysosome and interacts with Ub-Rheb for its deubiquitination in a manner dependent on the inactive Rag heterodimer in response to amino acid starvation."
Ubiquitin activates ATXN3.
| 4 1
| 4 1

sparser
"Further studies indicate that ubiquitin-dependent activation of ataxin-3 at Lys-117 is important for its ability to reduce high molecular weight ubiquitinated species in cells."

reach
"AT3 is composed of a structured globular N-terminal domain, the Josephin domain (JD), which displays ubiquitin hydrolase activity, followed by a disordered C-terminal tail containing two ubiquitin interacting motifs (UIMs) and the polyQ stretch of variable length, whose expansion beyond a certain threshold triggers SCA3 [XREF_BIBR, XREF_BIBR]."

reach
"Further studies indicate that ubiquitin dependent activation of ataxin-3 at Lys 117 is important for its ability to reduce high molecular weight ubiquitinated species in cells."

reach
"For SCA 3, it is known that both normal and polyQ expanded ataxin-3 localize to mitochondria [XREF_BIBR] and that degradation of polyQ expanded ataxin-3 via the UPS is promoted by an ubiquitin ligase in the outer mitochondrial membrane called MITOL [XREF_BIBR]."

reach
"Cytoplasmic Ubiquitin Specific Protease 19 (USP19) Modulates Aggregation of Polyglutamine Expanded Ataxin-3 and Huntingtin through the HSP90 Chaperone."
Ubiquitin inhibits ATXN3.
| 2
| 2

reach
"The research results from the Scaglione KM group demonstrate that the ubiquitin conjugating enzyme UBE2W attaches ubiquitin to the N-terminus of ataxin-3 and directs ubiquitin-degradation of ataxin-3 [24], suggesting that ubiquitin E2 enzyme may also directly bind to the substrate and promote its degradation."

reach
"Ataxin-3 limits ubiquitin chain length on CHIP substrates."
ATXN3 affects PRKN
7 1 1 | 30 11
ATXN3 binds PRKN.
7 1 | 8 11
7 1 | 8 9

reach
"Ataxin-3 interacts directly with Parkin and suppresses Parkin autoubiquitination via likely impeding the efficient charging of the E2 with Ub."

sparser
"The interaction between ataxin-3 and Parkin could, at least partially, explain the similarities between these diseases."

reach
"Parkin, an E3 implicated in Parkinson disease, also interacts functionally with ATXN3."

sparser
"Through its cysteine at residue 14, ataxin-3 alone can interact directly with the E2s used by parkin to self-ubiquitinate (Durcan et al., xref )."

No evidence text available

reach
"For example, understanding interactions between parkin and ataxin-3 might shed light on parkinsonian symptoms seen in MJD patients."

sparser
"The interaction between ataxin-3 and parkin is direct, involves multiple domains and is greatly enhanced by parkin self-ubiquitination."

sparser
"Biologically, phosphorylation at Ser-65 may inhibit the interaction between parkin and ataxin-3, allowing activated parkin to proceed with translocation to the mitochondria and substrate ubiquitination."

"The affinity of the polyglutamine fragment to Parkin was increased by Hsp70, in agreement with the hypothesis that molecular chaperones may play a role in the recruitment of misfolded proteins to the conjugation machinery (see, for example, Bercovich et al., 1997)."

No evidence text available
ATXN3 binds PRKN, Dnmt1, and UBL. 2 / 2
| 2

sparser
"For example, the interaction of the parkin Ubl domain with the UIM regions of the deubiquitinating protein ataxin-3 is required for the unique ubiquitin-editing role of ataxin-3 ( xref , xref )."

sparser
"The observation that the three UIM sites in ataxin-3 bind equally to a single site of the parkin Ubl domain suggests a multi- or polyvalent ligand binding mode."
ATXN3 deubiquitinates PRKN.
1 | 8
ATXN3 deubiquitinates PRKN. 9 / 9
1 | 8

reach
"Interestingly, although ataxin-3 deubiquitinates parkin, parkin is unable to ubiquitinate ataxin-3."

reach
"With parkin, ataxin-3 was unable to remove individual Ub moieties or preformed Ub chains after they had formed, suggesting that ataxin-3 was deubiquitinating parkin through a more unconventional mechanism."

reach
"ATXN3 deubiquitinates PARKIN directly and reduces the extent of PARKIN ubiquitination in cells [81]."

reach
"Ataxin-3, a DUB associated with Machado-Joseph disease, was reported to reduce the self ubiquitination of parkin, a familiar form of Parkinson disease associated E3 ubiquitin-ligase [XREF_BIBR]."

reach
"Thus, it is likely that Ataxin-3 inhibits parkin autoubiquitination by intercepting the Ub from E2 ~ Ub with its own active site thiol and the resulting DUB thioester intermediate is protected from hydrolysis by the stable ternary complex."

reach
"Wild-type and polyQ expanded ataxin-3 deubiquitinate parkin directly and parkin ubiquitinates and facilitates the clearance of wild-type and mutant ataxin-2 and ataxin-3 by proteasomal degradation [XREF_BIBR - XREF_BIBR]."

reach
"Ataxin-3 is proposed to reduce this self ubiquitination of Parkin by removal of the isopeptide linkage [XREF_BIBR]."

reach
"Recently, it has been shown that ataxin-3 deubiquitinates C-terminus of Hsp70 interacting protein (CHIP) and parkin XREF_BIBR, XREF_BIBR."

"?Moreover, ataxin-3 deubiquitinates parkin directly in vitro and in cells"
ATXN3 inhibits PRKN.
| 7
ATXN3 inhibits PRKN. 4 / 5
| 4

reach
"For example, mutant ataxin-3, the causative protein abnormality in SCA3, has been shown to enhance the autophagic degradation of parkin, which may in turn explain some of the parkinsonian features seen in this condition [XREF_BIBR]."

reach
"63 Interestingly, PolyQ expanded Ataxin-3 promotes degradation of Parkin via autophagy, 64 which helps explain why Parkin protein levels are decreased in the brain of transgenic mice overexpressing polyQ expanded but not wild type Ataxin-3."

reach
"We reported that the mutant form of ataxin-3, the protein responsible for MJD, promotes the autophagic degradation of parkin."

reach
"However, the MJF associated form of ataxin-3 promotes Parkin degradation in a proteasome independent manner [XREF_BIBR, XREF_BIBR]."
Mutated ATXN3 inhibits PRKN. 3 / 3
| 3

reach
"Mutant Ataxin-3, a polyglutamine dilatation associated with the onset of Machado-Joseph neurodegenerative disease, promotes Parkin degradation by autophagy and leads to a decrease in Parkin levels in in vivo [40]."

reach
"Mutant ataxin-3 promotes the autophagic degradation of parkin."

reach
"For example, mutant ataxin-3, the causative protein abnormality in SCA3, has been shown to enhance the autophagic degradation of parkin, which may in turn explain some of the parkinsonian features seen in this condition [XREF_BIBR]."
ATXN3 activates PRKN.
| 7
ATXN3 activates PRKN. 7 / 7
| 7

reach
"Hence, it is likely that ataxin-3 edits ubiquitin chains to target Parkin to different cellular pathways such as DNA repair and autophagy (see Section 3)."

reach
"In MJD, these partnership are not only disrupted, but the presence of the expanded ataxin-3 now promotes clearance of both parkin and CHIP, which over time can have deleterious consequences on neurons in MJD and PD."

reach
"Moreover, mutant but not wild-type ataxin-3 promotes the clearance of parkin via the autophagy pathway, raising the intriguing possibility that increased turnover of parkin may contribute to the pathogenesis of MJD and help explain some of the Parkinsonian features in MJD."

reach
"Remarkably, mutant but not wild-type ataxin-3 promotes the clearance of parkin via the autophagy pathway."

reach
"Supporting the notion that polyglutamine expansions alter ataxin-3 function, it was recently reported that pathogenic ataxin-3 targets the E3 ligase parkin for autophagic degradation, unlike wild-type ataxin-3, which rescues it through deubiquitination [XREF_BIBR]."

reach
"These findings were further confirmed and extended in cells, with the presence of the expanded form of ataxin-3 promoting clearance of parkin through the autophagy pathway."

reach
"As mutant, but not wild-type ataxin-3 promotes clearance of parkin via the autophagy pathway, there seems to be a possibility that increased turnover of parkin contributes to pathogenesis in MJD."
PRKN affects ATXN3
7 1 1 | 18 16
PRKN binds ATXN3.
7 1 | 8 11
7 1 | 8 9

reach
"Ataxin-3 interacts directly with Parkin and suppresses Parkin autoubiquitination via likely impeding the efficient charging of the E2 with Ub."

sparser
"The interaction between ataxin-3 and Parkin could, at least partially, explain the similarities between these diseases."

reach
"Parkin, an E3 implicated in Parkinson disease, also interacts functionally with ATXN3."

sparser
"Through its cysteine at residue 14, ataxin-3 alone can interact directly with the E2s used by parkin to self-ubiquitinate (Durcan et al., xref )."

No evidence text available

reach
"For example, understanding interactions between parkin and ataxin-3 might shed light on parkinsonian symptoms seen in MJD patients."

sparser
"The interaction between ataxin-3 and parkin is direct, involves multiple domains and is greatly enhanced by parkin self-ubiquitination."

sparser
"Biologically, phosphorylation at Ser-65 may inhibit the interaction between parkin and ataxin-3, allowing activated parkin to proceed with translocation to the mitochondria and substrate ubiquitination."

"The affinity of the polyglutamine fragment to Parkin was increased by Hsp70, in agreement with the hypothesis that molecular chaperones may play a role in the recruitment of misfolded proteins to the conjugation machinery (see, for example, Bercovich et al., 1997)."

No evidence text available
ATXN3 binds PRKN, Dnmt1, and UBL. 2 / 2
| 2

sparser
"For example, the interaction of the parkin Ubl domain with the UIM regions of the deubiquitinating protein ataxin-3 is required for the unique ubiquitin-editing role of ataxin-3 ( xref , xref )."

sparser
"The observation that the three UIM sites in ataxin-3 bind equally to a single site of the parkin Ubl domain suggests a multi- or polyvalent ligand binding mode."
PRKN ubiquitinates ATXN3.
1 | 2 5
PRKN ubiquitinates ATXN3. 8 / 8
1 | 2 5

sparser
"The possibility of redundancy in E3 ligase action is suggested by reports that overexpression of either CHIP ( xref ) or Parkin ( xref ) increases ubiquitination of polyglutamine-expanded ataxin-3 and reduces its cellular toxicity."

sparser
"Interestingly, although ataxin-3 deubiquitinates parkin, parkin is unable to ubiquitinate ataxin-3 (Durcan et al., xref , xref )."

reach
"Several E3 ubiquitin ligases, including the C-terminus of 70kDa heat-shock protein (Hsp70)-interacting protein (CHIP), parkin, and E4B, promote ataxin-3 ubiquitination."

reach
"Interestingly, although ataxin-3 deubiquitinates parkin, parkin is unable to ubiquitinate ataxin-3."

sparser
"Several E3 ubiquitin ligases (Durcan and Fon, xref ), including the C-terminus of 70kDa heat-shock protein (Hsp70)-interacting protein (CHIP), parkin, and E4B, promote ataxin-3 ubiquitination (Matsumoto et al., xref ; Jana et al., xref ; Miller et al., xref )."

sparser
"Parkin, an E3 ubiquitin ligase, promotes the ubiquitination and degradation of ATXN3, which is enhanced by Hsp70."

sparser
"Parkin also promotes ubiquitination and degradation of a polyglutamine-expanded ataxin-3 and reduces its cellular toxicity [ xref , xref ]."
PRKN activates ATXN3.
| 4
PRKN activates ATXN3. 4 / 4
| 4

reach
"Our results raise the possibility that parkin mediated K63 linked polyubiquitination may function upstream of ataxin-3 during aggresome formation."

reach
"Parkin selectively associates with and promotes the ubiquitylation and degradation of polyQ expanded GFP-polyQ fusion protein or ataxin-3 in vivo and in vitro."

reach
"Parkin also promotes ubiquitination and degradation of a polyglutamine expanded ataxin-3 and reduces its cellular toxicity [XREF_BIBR, XREF_BIBR]."

reach
"Although in this case, the E3 ligases that are acting redundantly to CHIP have not been identified, overexpression of either CHIP or parkin promotes the degradation of nNOS and polyglutamine expanded ataxin-3."
PRKN inhibits ATXN3.
| 2
PRKN inhibits ATXN3. 2 / 3
| 2

reach
"Overexpression of parkin reduces aggregation and cytotoxicity of an expanded polyglutamine ataxin-3 fragment."

reach
"Similarly, overexpression of the E3 ubiquitin ligase parkin induces ataxin-3 degradation and reduces aggregation and toxicity."
PRKN decreases the amount of ATXN3.
| 2
PRKN decreases the amount of ATXN3. 2 / 2
| 2

reach
"Parkin lowered the levels of nNOS and Q78 ataxin-3 but did not affect GR or AR112Q, suggesting that it acts redundantly to CHIP on some substrates."

reach
"Also, consistent with effects in non neuronal cells, both CHIP and Parkin decreased Q78 ataxin-3 levels in MN-1 neuronal cells but Mdm2 did not (XREF_FIG)."
TP53 affects ATXN3
5 | 13 17
TP53 binds ATXN3.
5 | 11 17
5 | 11 17

sparser
"Li-Fraumeni syndrome (LFS) is an autosomal dominant disease that is associated with germline TP53 mutations and it predisposes affected individuals to a high risk of developing multiple tumors."

reach
"We wonder whether the polyQ expansion affects the ATX-3 and p53 interaction."

reach
"We have found that ATX-3 interacts with p53 and functions as a novel p53 DUB."

sparser
"Therefore, both the Josephin and UIM domain coordinately regulate the interaction between ATX-3 and p53 ( xref )."

sparser
"The interaction between ATX-3 and p53 was confirmed under physiological condition ( xref ) and with in vitro purified forms ( xref ), indicating a direct association between these two proteins."

No evidence text available

sparser
"Consistently, as shown in xref , the cysteine 14 mutation did not affect the binding of ATX-3 to either native or ubiquitinated p53, whereas the ΔN mutant lost its binding to both forms of p53."

sparser
"Instructively, mutations effecting the expression of p53 are associated with Li Fraumini syndrome, an autosomal dominant hereditary cancer predisoposition syndrome."

sparser
"As ATX-3 interacts with p53 under physiological conditions and regulates the ubiquitination of p53 in cells, it is possible that ATX-3 may regulate the turnover of p53 via the Ub-proteasome pathway."

No evidence text available
TP53 activates ATXN3.
| 2
TP53 activates ATXN3. 2 / 2
| 2

reach
"Our data described in the present manuscript establish a mechanistic link between mutant ATXN3 expression and aberrant p53 pathway activation in SCA3, as previously reported [XREF_BIBR, XREF_BIBR]."

reach
"We report that persistent accumulation of DNA damage and strand breaks and chronic activation of the serine/threonine kinase ATM and the downstream p53 and protein kinase C-delta pro apoptotic pathways trigger neuronal dysfunction and eventually neuronal death in SCA3."
ATXN3 affects cell death
| 33
ATXN3 activates cell death.
| 29
| 23

reach
"Here, we report on the potential use of lithium carbonate and coenzyme Q10 (CoQ10) to reduce the cell death caused by the expanded ATX3 in a cultured cell model."

reach
"The truncated form of mutated ataxin-3 causes aggregation and cell death in vitro and in vivo."

reach
"Our observation demonstrated that the degradation of Hsp27 in SK-N-SH cells is not mediated by the proteasome degradation pathway.We have previously shown that expanded ataxin-3 leads to an increased [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Here, we report on the potential use of lithium carbonate and coenzyme Q10 to reduce cell death caused by the expanded ATX3 in cell culture."

reach
"For example, an expansion of the polyglutamine tract of the ataxin-3 protein, the target protein of SCA3, can lead to decreased Bcl-2 expression and enhance cell death via mitochondria dependent apopt[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Previously, it was shown that ataxin-3 is localized in the cytoplasmic compartment of cells and that expression of ataxin-3 with polyglutamine expansions causes cell death XREF_BIBR XREF_BIBR XREF_BIBR XREF_BIBR."

reach
"In fact, some cellular models of MJD show decreased antioxidant enzyme activity and increased mitochondrial mediated cell death via apoptosis."

reach
"Nevertheless, much remains unknown about how the CAG expansion in the ATXN3 gene causes brain dysfunction and cell death, manifesting in a characteristic clinical syndrome."

reach
"Indeed, a LOF of brat mitigates neither debcl- (this paper) nor hid- or Sca3 induced cell death [XREF_BIBR]."

reach
"In a SCA 3 cell model, the expression of a fragment of ataxin-3 containing an elongated polyQ stretch induced apoptosis and cell death as well as a severe ataxia in a mouse model, showing a more rapid manifestation of a SCA 3-reminiscent phenotype when compared to mice expressing full length mutant ataxin-3 [XREF_BIBR]."
Mutated ATXN3 activates cell death. 6 / 6
| 6

reach
"A neuroendocrine dysfunction, not testicular mutant ataxin-3 cleavage fragment or aggregate, causes cell death in testes of transgenic mice."

reach
"The mutant ataxin-3 forms intranuclear inclusions in cultured cells as well as in diseased human brain and also causes cell death in transfected cells."

reach
"Moreover, overexpression of PNKP in these cells blocked ATXN3-Q84-mediated caspase-3 activation (XREF_FIG), suggesting that the loss of PNKP function plays an important role in mutant ATXN3 mediated cell death."

reach
"Either PNKP overexpression or pharmacological inhibition of ATM dramatically blocked mutant ATXN3 mediated cell death."

reach
"In SCA3, full length mutant ATXN3 has been shown to increase mitochondrial mediated cell death in different models."

reach
"Coexpression of p21 (waf1/cip1/sdi1), a cyclin-Cdk inhibitor that induced cell cycle arrest in the G (1) phase, also increased the cell death susceptibility produced by the mutant ataxin-3 fragment in BHK-21 cells."
ATXN3 inhibits cell death.
| 4
| 4

reach
"Downregulation of ATXN3 enhanced AKT inhibitors (perifosine or MK-2206) induced cell death by BIM, but decreased the cell death induced by chemotherapeutic drugs (etoposide or cisplatin) via Bcl-xl."

reach
"These results are in complete agreement with previous reports showing that both depletion of ATXN3 and overexpression of pathogenic polyQ ATXN3 alleles caused disorganization of cytoskeleton and increased cell death in cultured cells (Neves-Carvalho et al., 2015; Rodrigues et al., 2010)."

reach
"In our cellular model, full-length expanded ataxin-3 that leads to neurodegenerative disorders significantly impaired the expression of Bcl-2 protein, which may be, at least in part, responsible for the weak tolerance to polyglutamine toxicity at the early stage of disease and ultimately resulted in an increase of stress induced cell death upon apoptotic stress."

reach
"Overexpression of ATXN3 in polyQ neurodegeneration models in Drosophila suppresses toxicity and cell death, implying that ATXN3 is a neuroprotective protein; its neuroprotective action, moreover, depends both on its DUB activity and proper functioning of the proteasome."
4 | 8 21
ATXN3 translocates.
| 7 17
ATXN3 translocates to the nucleus. 10 / 15
| 1 14

sparser
"Recent work in our lab demonstrated that Atxn3 responded to select proteotoxic stresses by altering its interactions with two shuttle proteins that function in protein degradation, valosin-containing protein (VCP/p97) and human Rad23 (HR23B); furthermore, in response to heat shock and oxidative stress Atxn3 translocated to the nucleus (Reina et al. xref )."

reach
"Since it has been shown that human ataxin-3 is translocated to the nucleus in cell lines exposed to heat shock, we also assessed the subcellular distribution of ATX-3 protein in a baseline condition and after heat shock in C. elegans."

sparser
"Upon stimulation of oxidative stress, ATXN3 and FoxO4 translocate to the nucleus and coordinately induce the expression of SOD2 ."

sparser
"Recruitment of ataxin-3 into the nucleus and formation of nuclear inclusion under two different conditions suggest that ataxin-3 may be translocated into the nucleus under certain conditions stressful on neuronal cells such as aging and polyglutamine neurotoxicity."

sparser
"Currently, it is not known how mutant ataxin-3 translocates into the nucleus."

sparser
"Heat-shock or oxidative stress can stimulate the nuclear translocation of ATXN3, where it has roles in transcriptional regulation, possibly through its deubiquitinase activity, and DNA repair ( xref A) ( xref )."

sparser
"Upon oxidative stress, ATXN3 and FOXO4 translocate to the nucleus, concomitantly bind to the SOD2 gene promoter and increase the expression of the antioxidant enzyme SOD2."

sparser
"The previously described translocation of ataxin-3 into the nucleus of cell lines following heat shock has been shown to be independent of HSF-1 xref , which suggests that alternative pathways may be related to ataxin-3's potential involvement in the stress response."

sparser
"The consistent presence of ataxin-3 in NIs could reflect a biological feature of wild-type ataxin-3, which is translocated into the nucleus under pathological conditions and participates in the formation of aggregates."

sparser
"In the present study we show two different conditions in which ataxin-3 is recruited into the nucleus and suggest a process to form nuclear inclusions."
ATXN3 translocates from the nucleus. 5 / 5
| 4 1

reach
"Because our data show that Atx3 can also be exported from the nucleus and that this export is at least partially mediated by the CRM1 pathway (see above), the similar localization of wild-type Atx3 and the R282T mutant might be due to the presence of competitive nuclear export signals."

reach
"Furthermore, using an in vivo nuclear export assay we show that Atx3 is actively exported from the nucleus and mapped this export activity to its N-terminal domain."

sparser
"Furthermore, using an in vivo nuclear export assay we show that Atx3 is actively exported from the nucleus and mapped this export activity to its N-terminal domain."

reach
"Our data show that Atx3 is actively imported to and exported from the cell nucleus, and that its nuclear export activity is dependent on a motif located at its N-terminal region."

reach
"Ataxin-3 is actively imported to and exported from the cell nucleus [XREF_BIBR]."
ATXN3 translocates from the cytosol. 2 / 2
| 1 1

sparser
"Nuclear inclusions formed by the glutamine-expanded Ataxin1 protein were capable of recruiting the unrelated Ataxin3 protein from the cytosol into the same nuclear inclusions. xref More recent work has begun to link disease protein relocation with proteotoxicity."

reach
"Nuclear inclusions formed by the glutamine expanded Ataxin1 protein were capable of recruiting the unrelated Ataxin3 protein from the cytosol into the same nuclear inclusions."
ATXN3 translocates from the cytoplasm. 2 / 2
| 1 1

reach
"We demonstrate that full-length ataxin-3 is readily recruited from the cytoplasm into NI seeded either by a pathologic ataxin-3 fragment or by a second unrelated glutamine-repeat disease protein, ataxin-1."

sparser
"We demonstrate that full-length ataxin-3 is readily recruited from the cytoplasm into NI seeded either by a pathologic ataxin-3 fragment or by a second unrelated glutamine-repeat disease protein, ataxin-1."
ATXN3 binds.
4 | 1 4
4 | 1 4

sparser
"To establish if this decrease was specific for spinocerebellar ataxia type 3 and associated with the function of ataxin-3, we investigated the levels of both Usp15 mRNA and protein in a mouse model of another polyglutamine disorder, Huntington’s disease (Schilling et al. , xref )."

sparser
"Genetic linkage analysis of the autosomal dominant hereditary form of MJD in the family localized the gene to 14q32.1, named SCA-3[ xref xref ]."

reach
"Genes that were part of this signature on the brain side were the TCF4, ATN1, PPP2R2B, ATXN10 and ATXN3, which are associated with neurodegenerative and developmental disorders such as Pitt-Hopkins Syndrome [XREF_BIBR], Dentatorubral pallidoluysian atrophy [XREF_BIBR], SCA12 [XREF_BIBR], SCA10 [XREF_BIBR] and SCA3 [XREF_BIBR]."

No evidence text available

sparser
"Notably, the SCA3 pathogenic form of ataxin-3 (ataxin-3(exp)) impairs the misfolded protein clearance via mechanisms that are either dependent or independent of its deubiquitinase (DUB) activity, resulting in the accumulation of misfolded proteins and the progressive loss of neurons in SCA3."

No evidence text available

No evidence text available

No evidence text available

sparser
"We tackled the interaction of the SCA3 protein, ataxin-3, with its direct binding partner, VCP, and the effect of this interaction in Drosophila ."
NEDD8 affects ATXN3
3 | 15 18
3 | 15 18

reach
"We demonstrated that the interaction between NEDD8 and ATXN3 is specific and independent of the presence of the UIM domains and the polyglutamine tract, since it occurs through its JD."

reach
"Merged images show colocalization of ATXN3 and NEDD8, both in the nucleus and cytoplasm, which is compatible with the possibility that the two proteins are interacting partners.In order to investigate[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Altogether, these results suggest that endogenous ATXN3 specifically interacts with NEDD8."

No evidence text available

sparser
"We also mapped the minimum domain required for ATXN3 to interact with NEDD8 using the pull-down assays."

sparser
"The fact that ATXN3 interacts with the UBL domain of HHR23 proteins and the recent finding that NEDD8 is present in ubiquitylated inclusions in the brain of MJD patients led us to investigate whether[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Interestingly, the homologue of ATXN3, atx-3, also interacts with NEDD8, indicating that this protein interaction is evolutionarily conserved and, thus, of functional relevance."

sparser
"Molecular docking studies of the NEDD8 molecule to the Josephin domain of ataxin-3 suggest that NEDD8 interacts with ataxin-3 in a substrate-like mode."

reach
"As shown in Fig. 2 b, full-length ATXN3 interacts in vitro with NEDD8, further indicating that ATXN3 can bind NEDD8 directly."

sparser
"Our data show that ATXN3 interacts specifically with NEDD8, and that the interaction site involves its catalytic JD."
L-glutamine affects ATXN3
| 32 2
L-glutamine activates ATXN3.
| 32
| 32

reach
"Spinocerebellar ataxia type 3 (SCA3) is caused by an expanded polyglutamine stretch in ataxin-3."

reach
"For example, SCA3 is caused by abnormal expansion of the polyglutamine in the C-term region of the protein, and the cleavage of the C-term fragment by caspases has been suggested to be essential for pathology of the disease XREF_BIBR."

reach
"Machado-Joseph disease (MJD, also known as spinocerebellar ataxia type 3, SCA3), an autosomal dominant neurological disorder, is caused by an abnormal expanded polyglutamine (polyQ) repeat in the ataxin-3 protein."

reach
"Hsp104 Suppresses Polyglutamine Induced Degeneration Post Onset in a Drosophila MJD and SCA3 Model."

reach
"The deubiquitinating enzyme Ataxin-3 (ATXN3) contains a polyglutamine (PolyQ) region, the expansion of which causes spinocerebellar ataxia type-3 (SCA3)."

reach
"Spinocerebellar ataxia type 3 (SCA3), the most common spinocerebellar ataxia, is caused by a polyglutamine (polyQ) expansion in the protein ataxin-3 (ATXN3)."

reach
"A polyglutamine expansion mutation in ataxin-3 causes spinocerebellar ataxia type 3 (SCA3), thereby providing a further link between ubiquitin dependent protein quality control mechanisms and neurodegeneration XREF_BIBR, XREF_BIBR."

reach
"Spinocerebellar ataxia (also called Machado-Joseph disease) is a late-onset neurodegenerative disease caused by the expansion of polyglutamine (CAG) repeats in the MJD1 gene."

reach
"SCA1, SCA2, MJD and SCA3, SCA6, SCA, and SCA17 are caused by polyglutamine encoding CAG repeat expansions."

reach
"Spinocerebellar ataxia type 3 (SCA3, also known as Machado-Joseph disease), a hereditary neurodegenerative disease, is caused by an abnormal expansion of the polyglutamine tract in the causative ATXN3 protein."
| 2

sparser
"VCP also has many associations with neurodegenerative disease as a component of intranuclear and cytoplasmic aggregates ( xref ), as the mutated gene in the rare neurological disorder inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia ( xref ), and through binding to Atx-3 and other polyglutamine proteins ( xref ; xref )."

sparser
"The TERA/VCP/p97 protein also interacts with a normal length polyglutamine (polyQ) tract sequence derived from a transcription factor, Brn-2 xref , and the mutant form of a polyglutamine disease protein, ataxin-3 (ATXN3) xref , which causes spinocerebellar ataxia type 3 (SCA3)."
UBC affects ATXN3
26 | 4
26 | 4

No evidence text available

No evidence text available

No evidence text available

No evidence text available

sparser
"UIMs enable ataxin-3 to bind poly-ubiquitin ( xref ; xref ; xref )."

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
CHEK1 affects ATXN3
| 16 9
CHEK1 binds ATXN3.
| 14 9
| 14 9

reach
"In contrast, treatment with ETO, which has little effect on Chk1 expression, did not change the interaction between ATX3 and Chk1 significantly (XREF_SUPPLEMENTARY)."

reach
"In support of our idea that S345 phosphorylation is involved in regulating the interaction between ATX3 and Chk1 after persistent replication stress, the decreased association between ATX3 and Chk1 under replicative stress was restrained when Chk1 S345 phosphorylation was inhibited by CGK733, an inhibitor of ATM and ATR."

reach
"We have demonstrated the ATX3 and Chk1 interaction under unperturbed conditions."

reach
"Under unperturbed conditions and upon DNA damage, ataxin-3 (ATX3) interacts with Chk1 and protects it from DDB1 and CUL4A- and FBXO6 and CUL1 mediated polyubiquitination and subsequent degradation, thereby promoting DNA repair and checkpoint signaling."

reach
"We wondered whether polyQ expansion compromises the interaction between ATX3 and Chk1."

sparser
"In contrast, treatment with ETO, which has little effect on Chk1 expression, did not change the interaction between ATX3 and Chk1 significantly ( xref )."

reach
"Ser345 phosphorylation which targets Chk1 for polyubiquitination and degradation plays an essential role in regulating the interaction between Chk1 and ATX3."

sparser
"Under unperturbed conditions and upon DNA damage, ataxin-3 (ATX3) interacts with Chk1 and protects it from DDB1/CUL4A- and FBXO6/CUL1-mediated polyubiquitination and subsequent degradation, thereby promoting DNA repair and checkpoint signaling."

reach
"Given that S345 phosphorylation of Chk1, mediated by ATR, targets Chk1 for degradation via the ubiquitin-proteasome pathway, we next assessed the impact of mutation of this phosphorylation site on the ATX3 and Chk1 interaction."

sparser
"To further define the interaction between ATX3 and Chk1, we generated a series of truncations of ATX3 and Chk1, respectively."
CHEK1 activates ATXN3.
| 2
CHEK1 activates ATXN3. 2 / 2
| 2

reach
"To clarify whether Chk1 is a direct target of ATX3, we determined if the two proteins co-purify in immunoprecipitation experiments using ectopically expressed tagged proteins in human 293T cells."

reach
"Our data demonstrate ATX3 to be a novel regulator of Chk1 stability, supporting a critical role of ATX3 in genome integrity maintenance via Chk1 stabilization."
BECN1 affects ATXN3
| 11 19
BECN1 binds ATXN3.
| 7 19
| 5 19

sparser
"The normal length polyQ region mediates binding of ataxin-3 to beclin-1, preventing its degradation and allowing it to stimulate autophagy (Fig. 2, top line)."

sparser
"PolyQ-expanded mutant ATXN3 similarly binds to Beclin1, but appears to facilitate its delivery and degradation by the proteasome, resulting in reduced Beclin 1 levels ( xref )."

sparser
"Interestingly, expanded polyQ tracts in other polyglutamine disease proteins compete with the shorter ATXN3 polyQ stretch and interfere with the ATXN3-BECN1 interaction."

reach
"Short polyQ domain enables interaction between ataxin-3 and beclin-1 [105]."
| PMC

sparser
"Indeed, overexpression of GFP-polyQ constructs decreased ataxin-3-beclin 1 binding ( xref )."

sparser
"Furthermore, the polyglutamine expansion of ataxin-3 impairs interaction of wild-type ataxin-3 with beclin1, promoting its degradation and thus decreasing the formation of autophagosomes [ xref ]."

sparser
"Short polyQ domain enables interaction between ataxin-3 and beclin-1 [105]."
| PMC

reach
"The normal length polyQ region mediates binding of ataxin-3 to beclin-1, preventing its degradation and allowing it to stimulate autophagy (Fig. 2, top line)."

sparser
"The ATXN3-Beclin1 interaction stabilize Beclin1, preventing its degradation by the proteasome ( xref )."

sparser
"Long polyglutamine expansion in mutant ataxin-3 competes with wide type ataxin-3 interaction with beclin 1 and leads to impaired starvation-induced autophagy."
BECN1 binds mutated ATXN3. 2 / 2
| 2

reach
"Thus, the longer polyQ stretches in mutant ataxin-3 bind beclin 1 more strongly than wild-type ataxin-3 but the mutant protein also has decreased deubiquitinase activity."

reach
"PolyQ expanded mutant ATXN3 similarly binds to Beclin1, but appears to facilitate its delivery and degradation by the proteasome, resulting in reduced Beclin 1 levels."
BECN1 activates ATXN3.
| 4
BECN1 activates mutated ATXN3. 2 / 2
| 2

reach
"Additional evidence supporting autophagy induction as a viable therapeutic target is the fact that lentiviral mediated overexpression of the autophagy protein beclin-1 increases clearance of mutant ATXN3 and decreases aggregates in a MJD rat model."

reach
"As a corollary, overexpression of beclin 1 can stimulate the autophagic flux and promote clearance of the mutant ataxin-3 in brains of SCA3 mouse models."
BECN1 activates ATXN3. 2 / 2
| 2

reach
"Beclin-1 stabilization is also promoted by USP19 and ataxin 3, which specifically removes K11- and K48-ubiquitin chain from Belcin-1, respectively [38, 39]."

reach
"A case in this regard is a recent study which showed that the polyQ domain of wild-type ataxin 3 enables it to interact with beclin 1, a key initiator of autophagy, allowing the deubiquitinase activity of ataxin 3 to protect beclin 1 from proteasome mediated degradation and thereby enabling autophagy."
ATXN3 affects UBC
26 | 4
26 | 4

No evidence text available

No evidence text available

No evidence text available

No evidence text available

sparser
"UIMs enable ataxin-3 to bind poly-ubiquitin ( xref ; xref ; xref )."

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
ATXN2 affects ATXN3
| 18 12
ATXN2 binds ATXN3.
| 5 12
| 5 4

sparser
"Interestingly, ataxin-2 binds to SCA3, which is responsible for SCA3, another spinal cerebellar ataxia, and enhances the progression of neurodegeneration in flies."

reach
"We also analyzed the possibility of genetic interactions between ATXN1 and ATXN2, ATXN2 and ATXN3, and ATXN2 and CACNA1A."

sparser
"Protein interaction studies indicate that Atx2 and Atx3 do not interact directly; in a survey of the interaction network of ataxia-associated proteins, Atx2 and Atx3 were separated by four nodes [ xref ]."

reach
"This was of particular interest in the case of the Atx2 and Atx3 interaction, given that Atx2 had previously been identified as a modifier of Atx1."

sparser
"The Interaction between Atx2 and Pathogenic Atx3 Is Dependent on the PAM2 Motif of Atx2."

sparser
"It has been hypothesized that the polyglutamine-expanded forms of ataxin-2 (in SCA2) and ataxin-3 (in SCA3), but not the wild-type, bind to ITPR1 and potentiate mGluR1-mediated calcium release from the ER [ xref , xref ]."

reach
"In this immunohistochemical study, we showed recruitment of ataxin-2, ataxin-3 and TATA box binding protein (TBP) into NIIs of the pontine neurons of spinocerebellar ataxia type (SCA) 1, SCA2, SCA3 and dentatorubral-pallidoluysian atrophy brains."

reach
"These data are consistent with and expand upon clinical findings suggesting interactions between Atx2 and Atx3 in human disease [XREF_BIBR - XREF_BIBR]."

reach
"16 Interestingly, ataxin-2 binds to SCA3, which is responsible for SCA3, another spinal cerebellar ataxia, and enhances the progression of neurodegeneration in flies."
| 3

sparser
"Furthermore, we show that this binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA, similar to what we have shown previously for HTT (Krauss et al., xref ), induces translation in a CAG repeat length-dependent manner in vitro and in cell lines."

sparser
"To test if the flanking regions influence the binding of MID1 to CAG repeat mRNAs, we tested the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNAs."

sparser
"Furthermore, we show that functionally, in line with what we have previously observed for HTT, the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA induces protein synthesis in a repeat length-dependent manner."
ATXN2 binds HTT, ITPR1, and ATXN3. 3 / 3
| 3

sparser
"Importantly, mutant HTT, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of IP3R1 in rat neurons and increase its sensitivity to InsP3 [ xref , xref ]."

sparser
"My laboratory discovered that mutant Huntingtin, ataxin-2 and ataxin-3 proteins specifically binds to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP 3 R1), an intracellular Ca 2+ release channel."

sparser
"Our laboratory discovered that mutant huntingtin, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP(3)R1), an intracellular Ca(2+) release channel."
| 2

sparser
"In this study we show that MID1 can also bind to the CAG repeat region of ATXN2, ATXN3 , and ATXN7 in vitro , suggesting that binding of MID1 to CAG repeats is not dependent on the flanking regions."

sparser
"We show that ATXN2, ATXN3 , and ATXN7 bind to MID1 in a CAG repeat length-dependent manner."
ATXN2 activates ATXN3.
| 11
ATXN2 activates ATXN3. 10 / 11
| 11

reach
"At the 24-h time point, coexpression of normal Atx2 with pathogenic Atx3 caused the early appearance of SDS-insoluble Atx3 complexes that remain within the stacking gel (compare lanes 1 and 2), presumably reflecting protein accumulations that correlate with the early appearance of nuclear inclusions in cryosections."

reach
"Atx-2 was found to mediate the pathogenic effects of SCA-1 and SCA-3."

reach
"Up-regulation of atx2 synergistically enhanced SCA3 degeneration, and strikingly, we found that the endogenous activity of atx2 modulates progression of neurodegeneration induced by pathogenic Atx3."

reach
"Ataxin gene variants in ATXN1, ATXN2, ATXN3, and ATXN7 cause SCA1, SCA2, SCA3, and SCA7, respectively."

reach
"XREF_BIBR SCA1, SCA2, Machado-Joseph or SCA3, SCA6, SCA7, SCA12, SCA17, and dentatorubral-pallidoluysian atrophy (DRPLA) are caused by (CAG) n repeat expansions in the ATXN1, ATXN2, ATXN3, CACNA1A, ATXN7, PPP2R2B, TBP, and ATN1 genes, respectively, and all lead to the expansion of a polyglutamine tract in the corresponding proteins."

reach
"These observations indicated that PABP has the opposite activity as Atx2 with respect to Atx3 dependent neurodegeneration : whereas Atx2 enhances the toxicity of Atx3, PABP is protective."

reach
"In the fly, endogenous Atx2 colocalized with pathogenic Atx3 in inclusions, as seen in human patients [XREF_BIBR], with up-regulation of Atx2 enhancing Atx3 toxicity concomitant with a faster onset of inclusions and of SDS-insoluble complexes."

reach
"Up-Regulation of Atx2 Synergistically Enhances Atx3 Induced Neurodegeneration."

reach
"Moreover, up-regulation of atx2, the Drosophila ortholog of the human gene that causes SCA2 disease, has been shown to enhance the toxicity of human disease forms of SCA1 and SCA3 in flies [XREF_BIBR]."

reach
"SCA2 and SCA3 are caused by polyglutamine expansions in ataxin2 and ataxin3, respectively [XREF_BIBR, XREF_BIBR]."
ATXN2 inhibits ATXN3.
| 2
ATXN2 inhibits mutated ATXN3. 2 / 2
| 2

reach
"Re-establishment of ataxin-2 levels reduces mutant ataxin-3 and alleviates Machado-Joseph disease pathogenesis opening a new avenue for therapeutic intervention in this and potentially other polyQ disorders."

reach
"Re-establishing ataxin-2 downregulates translation of mutant ataxin-3 and alleviates Machado-Joseph disease."
ATXN3 affects BECN1
| 10 19
ATXN3 binds BECN1.
| 7 19
| 5 19

sparser
"The normal length polyQ region mediates binding of ataxin-3 to beclin-1, preventing its degradation and allowing it to stimulate autophagy (Fig. 2, top line)."

sparser
"PolyQ-expanded mutant ATXN3 similarly binds to Beclin1, but appears to facilitate its delivery and degradation by the proteasome, resulting in reduced Beclin 1 levels ( xref )."

sparser
"Interestingly, expanded polyQ tracts in other polyglutamine disease proteins compete with the shorter ATXN3 polyQ stretch and interfere with the ATXN3-BECN1 interaction."

reach
"Short polyQ domain enables interaction between ataxin-3 and beclin-1 [105]."
| PMC

sparser
"Indeed, overexpression of GFP-polyQ constructs decreased ataxin-3-beclin 1 binding ( xref )."

sparser
"Furthermore, the polyglutamine expansion of ataxin-3 impairs interaction of wild-type ataxin-3 with beclin1, promoting its degradation and thus decreasing the formation of autophagosomes [ xref ]."

sparser
"Short polyQ domain enables interaction between ataxin-3 and beclin-1 [105]."
| PMC

reach
"The normal length polyQ region mediates binding of ataxin-3 to beclin-1, preventing its degradation and allowing it to stimulate autophagy (Fig. 2, top line)."

sparser
"The ATXN3-Beclin1 interaction stabilize Beclin1, preventing its degradation by the proteasome ( xref )."

sparser
"Long polyglutamine expansion in mutant ataxin-3 competes with wide type ataxin-3 interaction with beclin 1 and leads to impaired starvation-induced autophagy."
BECN1 binds mutated ATXN3. 2 / 2
| 2

reach
"Thus, the longer polyQ stretches in mutant ataxin-3 bind beclin 1 more strongly than wild-type ataxin-3 but the mutant protein also has decreased deubiquitinase activity."

reach
"PolyQ expanded mutant ATXN3 similarly binds to Beclin1, but appears to facilitate its delivery and degradation by the proteasome, resulting in reduced Beclin 1 levels."
ATXN3 activates BECN1.
| 3
ATXN3 activates BECN1. 3 / 3
| 3

reach
"According with the literature, the polyQ stretch in wild-type ataxin-3 induces autophagy by protecting Beclin-1 from proteasome-mediated degradation (Ashkenazi et al., 2017)."

reach
"Beclin-1 stabilization is also promoted by USP19 and ataxin 3, which specifically removes K11- and K48-ubiquitin chain from Belcin-1, respectively [38, 39]."

reach
"On the other hand, the ratio of LC3-II and LC3-I and LC3 total levels (XREF_FIG, D) are enhanced in MJD fibroblasts overexpressing beclin-1, particularly in the presence of chloroquine, reaching similar levels as CTRL fibroblasts (XREF_FIG), which indicates that although the number of autophagosomes is decreased, the autophagic flux can be restored."
HSJ1a affects ATXN3
| 3 22 2
HSJ1a activates ATXN3.
| 10
HSJ1a activates ATXN3. 8 / 8
| 8

reach
"These results suggest that HSP70 is directly involved in the degradation of Atx3 modulated by HSJ1a."

reach
"Based on our previous finding that HSJ1a mainly suppresses the Atx3 degradation through its UIM domain 45, we over-expressed the UIM domain mutant of HSJ1a (HSJ1a-UIM mut) and revealed that the mutant failed to reverse the reduction of soluble Atx3."

reach
"To investigate whether HSP70 is a direct mediator for degradation of Atx3 modulated by HSJ1a, we treated the cells with ATPase inhibitors of HSP70 XREF_BIBR."

reach
"Together, these data reaffirm that HSJ1a suppresses the degradation of Atx3 through the UIM domain, and thus suggest that it is able to reverse the reduction of soluble Atx3 caused by the PQE proteins."

reach
"As known, ubiquitination of a protein substrate is required for its proteasomal degradation XREF_BIBR, XREF_BIBR, thus the degradation of Atx3 modulated by HSJ1a may also be associated with ubiquitination of Atx3."

reach
"Thus we propose that the degradation of Atx3 modulated by HSJ1a may undergo through a proteasome pathway."

reach
"HSJ1a suppresses the degradation of intracellular Atx3 through its UIM domain."

reach
"Taken together, these results demonstrate that HSJ1a down-regulates the degradation of Atx3 through accumulating the ubiquitinated Atx3 involved in the Ub-proteasome pathway."
HSJ1a activates ubiquitinated ATXN3. 2 / 2
| 2

reach
"As a result, both HSJ1a and HSJ1a-JD mut can promote accumulation of the ubiquitinated Atx3 in cells (XREF_FIG), as compared with the mock vector."

reach
"On the other hand, HSJ1a can also impede the proteasomal degradation of the ubiquitinated Atx3 to maintain the protein level when needed."
HSJ1a inhibits ATXN3.
| 3 6
HSJ1a inhibits ATXN3. 7 / 7
| 3 4

eidos
"Based on our previous finding that HSJ1a mainly suppresses the Atx3 degradation through its UIM domain45 , we over-expressed the UIM-domain mutant of HSJ1a ( HSJ1a-UIMmut ) and revealed that the mutant failed to reverse the reduction of soluble Atx3 ( Fig. 4D and Supplemental Fig. 3B ) ."

eidos
"HSJ1a suppresses the degradation of Atx3 through its UIM domain ."

reach
"It is possible that the J-domain of HSJ1a enhances the degradation of Atx3 through assisting release of the ubiquitinated Atx3 from the HSP70 and CHIP complex, instead of promoting its ubiquitination."

reach
"We previously revealed that HSJ1a promotes proteasomal degradation of Atx3 through interacting with HSP70 mainly by the function of the J domain, while its UIM domain binds to the Ub chains conjugated in Atx3 and maintains its protein level 45."

reach
"We examined whether the J-domain of HSJ1a promotes the degradation of Atx3 through a proteasome or lysosome pathway."

reach
"Our findings demonstrate that the N-terminal J-domain of HSJ1a promotes proteasomal degradation of Atx3, whereas the C-terminal UIM domain alleviates the degradation."

eidos
"Together , these data reaffirm that HSJ1a suppresses the degradation of Atx3 through the UIM domain , and thus suggest that it is able to reverse the reduction of soluble Atx3 caused by the PQE proteins ."
HSJ1a bound to HSPA inhibits ATXN3. 2 / 2
| 2

reach
"Our studies both in vitro and in cell model demonstrate that the N-terminal J-domain of HSJ1a binds HSP70 and enhances proteasomal degradation of Atx3 (XREF_FIG & XREF_FIG)."

reach
"HSJ1a binds HSP70 and promotes proteasomal degradation of Atx3."
HSJ1a increases the amount of ATXN3.
| 3
HSJ1a increases the amount of ATXN3. 3 / 3
| 3

reach
"Overexpression of HA tagged Atx3 71Q causes inclusion body formation in cells (XREF_FIG), which can be alleviated by overexpression of HSJ1a, the JD or DeltaJD fragment, although both HSJ1a and its DeltaJD fragment increase the Atx3 71Q levels (XREF_FIG)."

reach
"To get information whether the change of Atx3 amount caused by HSJ1a or its fragments in cells is due to the different protein expression efficiency, we examined the transcriptional levels of the constructs by measuring the mRNA amounts (XREF_SUPPLEMENTARY)."

reach
"As a result, HSJ1a can slightly increase the protein level of normal Atx3 (Atx3 22Q) as compared with the mock vector (XREF_FIG), suggesting that HSJ1a may have regulatory function on the fate of Atx3 via some yet unidentified pathways."
HSJ1a binds ATXN3.
| 1 2
ATXN3 binds HSJ1a. 3 / 3
| 1 2

reach
"Next, we tested whether HSJ1a, CHIP, HSP70 and Atx3 can form a complex in cells."

sparser
"HSJ1a binds ubiquitinated Atx3 and retards its degradation."

sparser
"As known, ubiquitination of a protein substrate is required for its proteasomal degradation xref , xref , thus the degradation of Atx3 modulated by HSJ1a may also be associated with ubiquitination of Atx3."
HSJ1a decreases the amount of ATXN3.
| 2
HSJ1a decreases the amount of ATXN3. 2 / 2
| 2

reach
"These results suggest that down-regulation of the Atx3 level by the J-domain of HSJ1a is mediated by HSP70 rather than HSP90."

reach
"The UIM mutation indeed abolishes the increasing effect of HSJ1a on the protein level of Atx3 (XREF_FIG, lane 4), exhibiting similar effect with the JD fragment that significantly decreases the level of Atx3 (XREF_FIG)."
RAD23A affects ATXN3
9 2 1 | 5 6
RAD23A binds ATXN3.
9 2 1 | 3 6
9 2 1 | 3 4

sparser
"As a control experiment, we tested the interaction between ATXN3 and HHR23A (b), and under the conditions used, we were able to replicate the interaction reported before ."

reach
"HHR23A can simultaneously bind ubiquitin chains and ataxin-3 XREF_BIBR, XREF_BIBR, XREF_BIBR."

No evidence text available

sparser
"In this study, we demonstrate that hHR23A and human VCP/p97 are able to directly interact with human ataxin-3; indeed, we confirm the association of endogenous ataxin-3 with endogenous hHR23A and VCP/p97 xref , xref , xref – xref ."

sparser
"VCP/p97 and hHR23A interact directly with ataxin-3."

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
| 2

sparser
"It has been reported that ATX3 interacts with HHR23A and HHR23B, homolog of DNA repair protein RAD23 ( xref ), and that ATX3 can be recruited to the DNA damage sites induced by laser microirradiation ( xref )."

sparser
"Initial studies found that ataxin-3 interacts with two proteins, HHR23A and HHR23B, that are both homologs of the DNA repair protein Rad23 [ xref ]."
RAD23A activates ATXN3.
| 2
RAD23A activates ATXN3. 2 / 2
| 2

reach
"HHR23A did not increase ataxin-3 DUB activity or change its substrate preference, in accordance with recent data by Nicastro and colleagues (2010)."

reach
"Here, we sought to investigate whether VCP and p97 or hHR23A, both of which are involved in protein degradation pathways, also enhanced ataxin-3 activity through direct interactions."
GSK3B affects ATXN3
1 4 1 | 11 5 3
GSK3B phosphorylates ATXN3.
1 1 | 7 5 3
GSK3B phosphorylates ATXN3 on S256. 10 / 11
1 1 | 2 4 3

sparser
"Our results imply that phosphorylation of serine 256 in ataxin-3 by GSK 3beta regulates ataxin-3 aggregation."

rlimsp
"Phosphorylation of ataxin-3 by glycogen synthase kinase 3beta at serine 256 regulates the aggregation of ataxin-3."

reach
"As shown in Fig. 2 C, conversion of the S256 to alanine in ataxin-3 completely abolished its phosphorylation by GSK 3beta, whereas other mutants were still phosphorylated by GSK 3beta, suggesting that[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

No evidence text available

rlimsp
"Here we show that S256 site in ataxin-3 is phosphorylated by GSK 3beta."

No evidence text available

reach
"In our present study, we demonstrate that GSK 3beta phosphorylates ataxin-3 at serine 256 in vitro."

sparser
"PolyQ-expanded ataxin-3 is phosphorylated by glycogen synthase kinase 3 beta (GSK3β) at S256, and this phosphorylation is decreased by polyQ expansion (Fei et al., xref )."

rlimsp
"Our results imply that phosphorylation of serine 256 in ataxin-3 by GSK 3beta regulates ataxin-3 aggregation."

sparser
"For example, ATX3 phosphorylation at Ser256 by GSK3β regulates ATX3 aggregation [ xref ]."
GSK3B phosphorylates ATXN3. 6 / 6
| 5 1

reach
"Importantly, phosphorylation of ataxin-3 by GSK3beta decreases aggregation, suggesting a protective effect in SCA3."

reach
"As ataxin-3 interacts with GSK 3beta, we further determine whether GSK 3beta phosphorylates ataxin-3."

reach
"Interestingly, GSK 3beta differentially phosphorylates normal ataxin-3 relative to expanded ataxin-3."

reach
"As phospho dead mutant of ataxin-3-Q80 enhances its aggregation ability and GSK 3beta differentially phosphorylates normal ataxin-3 relative to expanded ataxin-3, it is therefore possible that differe[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"For mapping the site (s) in ataxin-3 that is phosphorylated by GSK 3beta, we analyzed the potential phosphorylation motifs in ataxin-3."

sparser
"Importantly, phosphorylation of ataxin-3 by GSK3β decreases aggregation, suggesting a protective effect in SCA3."
GSK3B binds ATXN3.
4 | 4
4 | 4

No evidence text available

No evidence text available

No evidence text available

reach
"These results further support that ataxin-3 interacts with GSK 3beta."

reach
"To investigate if there is a physical interaction between ataxin-3 and GSK 3beta, we evaluated the binding of ataxin-3 with GSK 3beta using an in vitro GST pull-down assay."

No evidence text available

reach
"These data suggest that both normal and expanded ataxin-3 interact with GSK 3beta in vitro."

reach
"As ataxin-3 interacts with GSK 3beta, we further determine whether GSK 3beta phosphorylates ataxin-3."
ATXN3 affects RAD23A
9 2 1 | 3 6
9 2 1 | 3 4

sparser
"As a control experiment, we tested the interaction between ATXN3 and HHR23A (b), and under the conditions used, we were able to replicate the interaction reported before ."

reach
"HHR23A can simultaneously bind ubiquitin chains and ataxin-3 XREF_BIBR, XREF_BIBR, XREF_BIBR."

No evidence text available

sparser
"In this study, we demonstrate that hHR23A and human VCP/p97 are able to directly interact with human ataxin-3; indeed, we confirm the association of endogenous ataxin-3 with endogenous hHR23A and VCP/p97 xref , xref , xref – xref ."

sparser
"VCP/p97 and hHR23A interact directly with ataxin-3."

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
| 2

sparser
"It has been reported that ATX3 interacts with HHR23A and HHR23B, homolog of DNA repair protein RAD23 ( xref ), and that ATX3 can be recruited to the DNA damage sites induced by laser microirradiation ( xref )."

sparser
"Initial studies found that ataxin-3 interacts with two proteins, HHR23A and HHR23B, that are both homologs of the DNA repair protein Rad23 [ xref ]."
ATXN3 affects AP1S2
| 23
| 21

sparser
"Familial IGHD, however, is associated with at least four Mendelian disorders ( xref , xref , xref , xref , xref , xref , xref ), including two forms that have autosomal recessive inheritance (IGHD type IA, IB) as well as autosomal dominant (IGHD type II) and X−linked (IGHD III) forms. xref depicts the mutational spectrum of GHD, which is discussed in greater detail later in the review."

sparser
"Although the X-linked and autosomal dominant forms of FMD are very similar, there are distinctions to be made between the two conditions."

sparser
"Both the LIS1 gene (OMIM 601545) at 17p13.3 and the DCX gene (OMIM 300121) at Xq22.3, encoding for microtubule associated proteins, can cause classical LIS and/or SBH, inherited as autosomal dominant or X-linked dominant forms, respectively."

sparser
"Mutations causing both the X-linked and autosomal dominant forms of Kallmann’s syndrome (hypogonadotropic hypogonadism and anosmia), which is more common in males, were recently identified (KAL-1 and FGFR1, respectively)."
| PMC

sparser
"MPS is most frequently inherited in an autosomal recessive fashion but X-linked and autosomal dominant forms also occur."

sparser
"IGHD can be inherited as autosomal recessive (IGHD IA and IB), autosomal dominant (IGHD II) and X-linked (IGHD III) forms [4] ."

sparser
"Autosomal dominant and X-linked forms accounted for 21% and 8% for NON-RP and 15% and 8% for RP, respectively (Fig.  xref A-I)."

sparser
"Patterns of inheritance include autosomal recessive, autosomal dominant and X-linked forms."

sparser
"Many RP gene groups and forms exist: including autosomal recessive, autosomal dominant and X-linked forms."

sparser
"Of these, 3 genes were unlikely to be involved in tooth agenesis: PLAC4 is mainly involved in placenta tissue formation ( xref ); CHST8 has been associated with autosomal recessive peeling skin syndrome ( xref ); SYNGAP1 is responsible for non-syndromic mental retardation autosomal dominant form 5 (OMIM 612621) ( xref ), a phenotype not reported in this family."
| 2

sparser
"The list of the diseases associated with OPLL includes hypophosphatemic rickets/osteomalacia, including an autosomal dominant form (MIM 193100) caused by FGF23 mutations, an X-linked dominant form (MIM 307800) caused by PHEX mutations, an X-linked recessive form (MIM 300554) caused by CLCN5 mutations, and autosomal recessive forms caused by DMP1 (MIM 600980) and ENPP1 (MIM 173335) mutations."

sparser
"Several forms of hypophosphatemic rickets are known, including an X-linked form (MIM 307800) caused by phosphate regulating endopeptidase homolog, X-linked ( PHEX ) mutations (MIM 300550), an autosomal dominant form (MIM 193100) caused by fibroblast growth factor 23 ( FGF23 ) mutations (MIM 605380), an X-linked recessive form (MIM 300554) caused by chloride channel, voltage-sensitive 5 ( CLCN5 ) mutations (MIM 300008), and autosomal recessive forms caused by dentin matrix acidic phosphoprotein 1 ( DMP1 ) (MIM 600980), hypophosphatemic rickets, autosomal recessive 2 ( ARHR2 ) (MIM 613312) or ectonucleotide pyrophosphatase/phosphodiesterase 1 ( ENPP1 ) (MIM 173335) mutations. 'tiptoe walking' ( TTW ) mouse, which has a spontaneous nonsense mutation in ENPP1 is a good model for OPLL.[ xref ] Also, OPPL is a frequent complication in patients with endocrine disorders including hypoparathyroidism[ xref ] and acromegaly/gigantism.[ xref ] However, most cases of OPLL are idiopathic."
SQSTM1 affects ATXN3
2 | 6 14
SQSTM1 binds ATXN3.
2 | 4 14
2 | 4 14

sparser
"We also found that p62 can regulate aggresome formation of pathogenic ataxin-3, and p62 physically interacts with pathogenic ataxin-3, but not normal ataxin-3."

reach
"In this study we demonstrate that p62 directly interacts with pathogenic Machado Joseph Disease (MJD)-associated protein ataxin-3 with polyglutamine (polyQ) expansion."

sparser
"Thus, the role of mTOR signaling in p62-mediated aggresome formation of ataxin-3 needs to be further explored."

sparser
"In agreement with this hypothesis, our results showed a cytoprotective role of p62-mediated aggresome formation of pathogenic ataxin-3 ( xref )."

reach
"We also found that p62 can regulate aggresome formation of pathogenic ataxin-3, and p62 physically interacts with pathogenic ataxin-3, but not normal ataxin-3."

sparser
"In this study we demonstrate that p62 directly interacts with pathogenic Machado Joseph Disease (MJD)-associated protein ataxin-3 with polyglutamine (polyQ) expansion."

No evidence text available

sparser
"We observed multiple molecular phenotypes at the undifferentiated hESC stage, including the formation of p62-positive aggresomes containing the disease protein ATXN3 and altered expression of key regulators of protein homeostasis."

sparser
"This study includes the following findings: (1) p62 directly associates with ataxin-3-Q80, and co-localizes with ataxin-3-Q80 aggresomes ( xref and xref ); (2) p62 regulates ataxin-3-Q80 aggresome formation without affecting the protein level and self-aggregation of ataxin-3-Q80 ( xref , xref and xref ); (3) The regulation of ataxin-3-Q80 aggresome formation by p62 has a protective role against ataxin-3-Q80 triggered cell toxicity ( xref )."

sparser
"Also, we speculated that p62 could directly associate with ataxin-3-Q80 before targets ataxin-3-Q80 to the aggresomes."
SQSTM1 activates ATXN3.
| 2
SQSTM1 activates ATXN3. 2 / 2
| 2

reach
"In agreement with this hypothesis, our results showed a cytoprotective role of p62 mediated aggresome formation of pathogenic ataxin-3 (XREF_FIG)."

reach
"To further explore the biological function underlying p62 mediated ataxin-3 aggresome formation, we tested ataxin-3-induced cell death in control and p62 depleted cells."
| 22

sparser
"Collectively, the data in xref lead us to conclude that the interaction of ataxin-3 with Rad23 is necessary for the ability of this DUB to suppress polyQ-dependent degeneration in fly eyes, although it is not important for ataxin-3 to interact with polyQ78."

sparser
"The ataxin-3-Rad23 interaction also stabilizes the SCA3 protein by decelerating its proteasomal turnover; this process leads to higher levels of pathogenic ataxin-3 in Drosophila , but the higher levels are seemingly negated by the auto-protective role of ataxin-3 ( xref ; xref ). (Our ongoing, unpublished work indicates that Ubs1 also plays a protective role, potentially through mechanisms that involve pathogenic ataxin-3 binding certain ubiquitin species but not being able to process them)."

sparser
"Since reducing levels of polyQ proteins can decrease their toxicity, we tested whether genetically modulating the ataxin-3-Rad23 interaction regulates its toxicity in Drosophila."

sparser
"However, a function for the ataxin-3Rad23 interaction in regulating co-chaperone transcription has not been reported to date."

sparser
"Ubs2 facilitates not only ataxin-3’s interaction with ubiquitin to aid cleavage, but is also the site where the proteasome-associated protein, Rad23 binds ataxin-3 ( xref ; xref ; xref )."

sparser
"Collectively, the data in Fig. 2 lead us to conclude that the interaction of ataxin-3 with Rad23 is necessary for the ability of this DUB to suppress polyQ-dependent degeneration in fly eyes, althou[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Our analyses indicate that the ataxin-3Rad23 interaction is upstream of DnaJ-1."

sparser
"If ataxin-3 does not bind Rad23, it is then degraded."

sparser
"Therefore, for the current studies we wanted to ensure that any phenotypic effects observed from disrupting the ataxin-3Rad23 interaction were not due to simply lower ataxin-3 protein levels, but resulted from the interruption of the binding of these two proteins."

sparser
"Because atx3 binds the ubiquitin-like domain of rad23, which also mediates the interaction between rad23 and the proteasome ( xref ), it is likely that rad23 may not interact with atx3 and the proteasome simultaneously."
| 3

sparser
"Ataxin-3 interacts with ubiquitinated substrates, p97, Rad23, and the proteasome, and the expanded-polyglutamine-containing ataxin-3 is defective in the substrate degradation [74] ."

sparser
"Ataxin-3 binds directly to at least two PQC-related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; xref )."

sparser
"Ataxin-3 binds directly to at least two PQC-related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; Fig. 1 A)."
IGFALS affects ATXN3
| 22
| 22

sparser
"TARDBP-related ALS patients present as adult-onset, autosomal dominant form of ALS with predominant limb onset and a wide variation in the age of onset (30–77 yrs) and disease duration."

sparser
"Around 10% of the cases are inherited and ALS8 is an autosomal dominant form of familial ALS caused by mutations in the vamp-associated protein B/C ( VAPB ) gene."

sparser
"Amyotrophic lateral sclerosis type 4 (ALS4) is a rare, early-onset, autosomal dominant form of ALS, characterized by slow disease progression and sparing of respiratory musculature."

sparser
"An autosomal dominant form of ALS and FTD linked to chromosome 9p 13–22 ( xref ) related to GGGGCC hexanucleotide repeat expansion in intron 1 of the C9orf72 gene has recently been identified as the most common pathologic mutation in familial and sporadic ALS/FTD as well as in familial ALS and familial FTD ( xref , xref )."

sparser
"In conclusion, our study of an autosomal dominant form of ALS uncovered a function of R-loops."

sparser
"Mutations in the FUS gene have been identified as the primary cause of ALS 6, an autosomal dominant form of familial ALS (FALS) linked to chromosome 16."

sparser
"We have explored methods to maximize the amount of genetic information that can be derived from a small family with an autosomal dominant form of ALS and show that analysis of high density SNP haplotypes are able to precisely define nearly all regions of the genome that are shared among the affected individuals."

sparser
"Further supporting a role for ER morphogenesis in neurologic disorders, the vesicle-associated membrane-protein associated protein B (VAP-B) mutant P56S that underlies a autosomal dominant form of familial ALS is associated with the production of a novel form of organized SER ( xref )."

sparser
"ALS4 is a rare autosomal dominant form of juvenile-onset ALS due to mutations in SETX [ xref ]."

sparser
"Several missense mutations in the senataxin gene ( SETX ) (L389S, R2136H, and T3I) cause a rare autosomal dominant form of juvenile ALS (ALS4) characterized by distal muscle weakness and atrophy with pyramidal signs, in which sensation remains unaffected xref ."
HTT affects ATXN3
| 9 13
HTT binds ATXN3.
| 13
| 8

sparser
"In previous studies, we demonstrated that polyQ-expanded Huntingtin (Htt exp ) and ataxin-3 (Atx3 exp ) specifically bound to the InsP 3 R1 carboxy-terminal region ( xref ; xref )."

sparser
"The best studied family member, UBQLN1, also known as protein linking integrin-associated protein and cytoskeleton-1 (PLIC-1), is reported to interact with the polyQ disease proteins huntingtin (HTT) and ataxin-3 (ATXN3) ( xref ; xref )."

sparser
"Mutant HTT and ataxin-3 proteins form interactions that are difficult to disrupt using traditional small molecule drugs xref ."

sparser
"Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder that is associated with mutations in the huntingtin gene (htt) [ xref ]."

sparser
"Since DAPI shows modest selectivity and reasonable binding affinity for an RNA containing a single copy of the 5’C A G/3’G A C motif that is associated with HD and SCA3, we aimed to identify chemically similar compounds with improved affinities and selectivities."

sparser
"A recent report demonstrated that CHIP, a co-chaperone of Hsc70 and ubiquitin ligase for misfolded proteins, selectively binds to polyQ-expanded ataxin-3 or huntingtin but not to the normal proteins ([MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"The best studied family member, UBQLN1, also known as protein linking integrin-associated protein and cytoskeleton-1 (PLIC-1), is reported to interact with the polyQ disease proteins Huntingtin (HTT) [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"For instance, when mutant forms of either huntingtin (Huntington’s disease) or MJD1 (spinocerebellar ataxia type 3) were directed to the fly eye, each provoked late-onset degeneration preferential[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
ATXN2 binds HTT, ITPR1, and ATXN3. 3 / 3
| 3

sparser
"Importantly, mutant HTT, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of IP3R1 in rat neurons and increase its sensitivity to InsP3 [ xref , xref ]."

sparser
"My laboratory discovered that mutant Huntingtin, ataxin-2 and ataxin-3 proteins specifically binds to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP 3 R1), an intracellular Ca 2+ release channel."

sparser
"Our laboratory discovered that mutant huntingtin, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP(3)R1), an intracellular Ca(2+) release channel."
HTT binds ATXN3 and POLR2A. 2 / 2
| 2

sparser
"PNKP has been shown to promote transcription-coupled double-strand break repair ( xref ) and often performs transcription-coupled repair when in complex with huntingtin protein, RNA polymerase II subunit A, ataxin-3, and cyclic AMP-response element binding protein (CBP) ( xref )."

sparser
"The mechanisms for this were recently suggested to be through direct interactions between HTT and RNA polymerase II subunit A (POLR2A), ataxin-3, the DNA repair enzyme polynucleotide-kinase-3'-phosphatase (PNKP), and cyclic AMP-response element-binding (CREB) protein (CBP) [ xref ], as well as complexes with the DNA double-strand break repair complex Ku70/Ku80 [ xref ]."
HTT inhibits ATXN3.
| 3
HTT inhibits ATXN3. 3 / 3
| 3

reach
"Our data indicate that the N-terminal fragment of polyQ-expanded (PQE) Atx7 or Htt can coaggregate with and sequester AR and Atx3 into insoluble aggregates or inclusions through their respective polyQ tracts."

reach
"PQE Atx7 and Htt reduce the soluble fraction of endogenous Atx3 but increase its insoluble aggregates."

reach
"PQE Atx7 and Htt promote proteasomal degradation of intracellular Atx3."
HTT decreases the amount of ATXN3.
| 3
HTT decreases the amount of ATXN3. 3 / 3
| 3

reach
"Similarly, Htt 100Q -N90 could also reduce the total Atx3 level."

reach
"The results showed that, NLS-Atx7 93Q -N172 and Htt 100Q -N90 both caused reduction of the soluble Atx3 level, while MG132 treatment, to some extent, could reverse the decrease of Atx3 in the supernatant fraction."

reach
"PQE Atx7 and Htt reduce the overall level of intracellular Atx3."
HTT activates ATXN3.
| 3
HTT activates ATXN3. 3 / 3
| 3

reach
"However, blocking of the autophagic degradation exhibited no significant influence on the decline of Atx3 caused by Atx7 93Q -N172 or Htt 100Q -N90 (Supplemental Fig. 2B, D)."

reach
"The same group demonstrated the late-onset transgenic model with ataxin-3 driven by huntingtin promoter."

reach
"In this study we screened a cohort of 21 Greek patients with HD phenocopy syndromes formutations causing HDL2, SCA17, SCA1, SCA2, SCA3, SCA8, SCA12 and DRPLA."
| 22

sparser
"Collectively, the data in xref lead us to conclude that the interaction of ataxin-3 with Rad23 is necessary for the ability of this DUB to suppress polyQ-dependent degeneration in fly eyes, although it is not important for ataxin-3 to interact with polyQ78."

sparser
"The ataxin-3-Rad23 interaction also stabilizes the SCA3 protein by decelerating its proteasomal turnover; this process leads to higher levels of pathogenic ataxin-3 in Drosophila , but the higher levels are seemingly negated by the auto-protective role of ataxin-3 ( xref ; xref ). (Our ongoing, unpublished work indicates that Ubs1 also plays a protective role, potentially through mechanisms that involve pathogenic ataxin-3 binding certain ubiquitin species but not being able to process them)."

sparser
"Since reducing levels of polyQ proteins can decrease their toxicity, we tested whether genetically modulating the ataxin-3-Rad23 interaction regulates its toxicity in Drosophila."

sparser
"However, a function for the ataxin-3Rad23 interaction in regulating co-chaperone transcription has not been reported to date."

sparser
"Ubs2 facilitates not only ataxin-3’s interaction with ubiquitin to aid cleavage, but is also the site where the proteasome-associated protein, Rad23 binds ataxin-3 ( xref ; xref ; xref )."

sparser
"Collectively, the data in Fig. 2 lead us to conclude that the interaction of ataxin-3 with Rad23 is necessary for the ability of this DUB to suppress polyQ-dependent degeneration in fly eyes, althou[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Our analyses indicate that the ataxin-3Rad23 interaction is upstream of DnaJ-1."

sparser
"If ataxin-3 does not bind Rad23, it is then degraded."

sparser
"Therefore, for the current studies we wanted to ensure that any phenotypic effects observed from disrupting the ataxin-3Rad23 interaction were not due to simply lower ataxin-3 protein levels, but resulted from the interruption of the binding of these two proteins."

sparser
"Because atx3 binds the ubiquitin-like domain of rad23, which also mediates the interaction between rad23 and the proteasome ( xref ), it is likely that rad23 may not interact with atx3 and the proteasome simultaneously."
| 3

sparser
"Ataxin-3 interacts with ubiquitinated substrates, p97, Rad23, and the proteasome, and the expanded-polyglutamine-containing ataxin-3 is defective in the substrate degradation [74] ."

sparser
"Ataxin-3 binds directly to at least two PQC-related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; xref )."

sparser
"Ataxin-3 binds directly to at least two PQC-related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; Fig. 1 A)."
ATXN3 affects IGFALS
| 22
| 22

sparser
"TARDBP-related ALS patients present as adult-onset, autosomal dominant form of ALS with predominant limb onset and a wide variation in the age of onset (30–77 yrs) and disease duration."

sparser
"Around 10% of the cases are inherited and ALS8 is an autosomal dominant form of familial ALS caused by mutations in the vamp-associated protein B/C ( VAPB ) gene."

sparser
"Amyotrophic lateral sclerosis type 4 (ALS4) is a rare, early-onset, autosomal dominant form of ALS, characterized by slow disease progression and sparing of respiratory musculature."

sparser
"An autosomal dominant form of ALS and FTD linked to chromosome 9p 13–22 ( xref ) related to GGGGCC hexanucleotide repeat expansion in intron 1 of the C9orf72 gene has recently been identified as the most common pathologic mutation in familial and sporadic ALS/FTD as well as in familial ALS and familial FTD ( xref , xref )."

sparser
"In conclusion, our study of an autosomal dominant form of ALS uncovered a function of R-loops."

sparser
"Mutations in the FUS gene have been identified as the primary cause of ALS 6, an autosomal dominant form of familial ALS (FALS) linked to chromosome 16."

sparser
"We have explored methods to maximize the amount of genetic information that can be derived from a small family with an autosomal dominant form of ALS and show that analysis of high density SNP haplotypes are able to precisely define nearly all regions of the genome that are shared among the affected individuals."

sparser
"Further supporting a role for ER morphogenesis in neurologic disorders, the vesicle-associated membrane-protein associated protein B (VAP-B) mutant P56S that underlies a autosomal dominant form of familial ALS is associated with the production of a novel form of organized SER ( xref )."

sparser
"ALS4 is a rare autosomal dominant form of juvenile-onset ALS due to mutations in SETX [ xref ]."

sparser
"Several missense mutations in the senataxin gene ( SETX ) (L389S, R2136H, and T3I) cause a rare autosomal dominant form of juvenile ALS (ALS4) characterized by distal muscle weakness and atrophy with pyramidal signs, in which sensation remains unaffected xref ."
SUMO1 affects ATXN3
2 | 12 4
SUMO1 binds ATXN3.
2 | 3 1
2 | 3 1

sparser
"The interaction between ATX3 and SUMO1, mediated by N-terminal Josephin domain and further stimulated by DNA damage, is indispensable for the localization of ATX3 to DSBs (Pfeiffer et al., xref )."

No evidence text available

No evidence text available

reach
"We observed that endogenous ataxin-3 interacted with SUMO1, whereas hardly any interaction with SUMO2 could be detected under our assay conditions (Fig XREF_FIG A)."

reach
"In summary, our experiments show that ataxin-3 interacts with SUMO1 and that SUMOylation is required for the recruitment of ataxin-3 to DSBs."

reach
"Additionally, our findings reveal that ataxin-3 can directly interact with SUMO1 and that this interaction stimulates the deubiquitylating activity of the enzyme."
SUMO1 sumoylates ATXN3.
| 3 3
SUMO1 sumoylates ATXN3 on K166. 3 / 3
| 2 1

reach
"In SCA3, ATXN3 can be SUMOylated at site K166 by SUMO-1, which would enhance mutant ATXN3 stability without affecting aggregate formation."

sparser
"In addition, ATXN3 SUMOylation by SUMO-1 on site K166 also increases apoptosis in SCA3; therefore, SUMOylation by SUMO-1 might stimulate SCA3 pathogenesis through both effects described above."

reach
"In addition, ATXN3 SUMOylation by SUMO-1 on site K166 also increases apoptosis in SCA3; therefore, SUMOylation by SUMO-1 might stimulate SCA3 pathogenesis through both effects described above."
SUMO1 sumoylates ATXN3 on K356. 3 / 3
| 1 2

reach
"In addition to K166, ATXN3 can also be SUMOylated at site K356 by SUMO-1 and SUMO-2."

sparser
"In addition to K166, ATXN3 can also be SUMOylated at site K356 (Figure xref ) by SUMO-1 and SUMO-2."

sparser
"Ataxin-3 is SUMOylated by SUMO1 and SUMO2 at residue K356 ( xref ; xref )."
SUMO1 activates ATXN3.
| 4
SUMO1 activates ATXN3. 4 / 5
| 4

reach
"Interestingly, SUMO-1 overexpression enhanced the co-localization of ataxin-3 and autophagy marker LC3 without increasing LC3 puncta formation suggesting that SUMO-1 is involved in the substrate recruitment rather than the induction of autophagy."

reach
"In addition, we further confirmed SUMO-1 modification decreased the degradation and enhanced the stability of mutant-type ataxin-3 by chase assay."

reach
"By contrast, SUMO-1 coexpression significantly enhanced the colocalization of LC3 and ataxin-3 signals (highlighted by arrows) (XREF_FIG, upper panels)."

reach
"In addition to the SUMO dependent recruitment of ATXN3, free SUMO1 can also stimulate ATXN3 DUB activity against Ub-K63 chain in vitro."
SUMO1 inhibits ATXN3.
| 2
SUMO1 inhibits ATXN3. 2 / 2
| 2

reach
"In conclusion, we present evidence that SUMO-1 induces ataxin-3 degradation via autophagy although UPS also contribute to this process to a certain extent."

reach
"These results together indicate that SUMO-1 promotes the degradation of ataxin-3 via autophagy and the putative SIM of ataxin-3 plays a role in this process."
ATXN3 affects SQSTM1
2 | 4 14
2 | 4 14

sparser
"We also found that p62 can regulate aggresome formation of pathogenic ataxin-3, and p62 physically interacts with pathogenic ataxin-3, but not normal ataxin-3."

reach
"In this study we demonstrate that p62 directly interacts with pathogenic Machado Joseph Disease (MJD)-associated protein ataxin-3 with polyglutamine (polyQ) expansion."

sparser
"Thus, the role of mTOR signaling in p62-mediated aggresome formation of ataxin-3 needs to be further explored."

sparser
"In agreement with this hypothesis, our results showed a cytoprotective role of p62-mediated aggresome formation of pathogenic ataxin-3 ( xref )."

reach
"We also found that p62 can regulate aggresome formation of pathogenic ataxin-3, and p62 physically interacts with pathogenic ataxin-3, but not normal ataxin-3."

sparser
"In this study we demonstrate that p62 directly interacts with pathogenic Machado Joseph Disease (MJD)-associated protein ataxin-3 with polyglutamine (polyQ) expansion."

No evidence text available

sparser
"We observed multiple molecular phenotypes at the undifferentiated hESC stage, including the formation of p62-positive aggresomes containing the disease protein ATXN3 and altered expression of key regulators of protein homeostasis."

sparser
"This study includes the following findings: (1) p62 directly associates with ataxin-3-Q80, and co-localizes with ataxin-3-Q80 aggresomes ( xref and xref ); (2) p62 regulates ataxin-3-Q80 aggresome formation without affecting the protein level and self-aggregation of ataxin-3-Q80 ( xref , xref and xref ); (3) The regulation of ataxin-3-Q80 aggresome formation by p62 has a protective role against ataxin-3-Q80 triggered cell toxicity ( xref )."

sparser
"Also, we speculated that p62 could directly associate with ataxin-3-Q80 before targets ataxin-3-Q80 to the aggresomes."
RP affects ATXN3
| 19
ATXN3 binds RP. 10 / 19
| 19

sparser
"Here we use this model to investigate two pathogenic mutations in PRPF31, SP117 and AD5, causing the autosomal dominant form of RP."

sparser
"A major cause of the autosomal dominant form of RP (ADRP) is mutation of the rhodopsin gene [1, 2] ."

sparser
"In 1981, Heckenlively et al xref presented the initial clinical description of a family believed to have an autosomal dominant form of RP."

sparser
"For patient 9, the variation predicted as pathogenic allowed the molecular diagnosis conclusion to be probably positive for the autosomal dominant form of RP."

sparser
"In this RP cohort, symptoms were first reported at 5 years of age, thus confirming the earlier onset of RPGR -RP, particularly compared to autosomal dominant RP forms. xref , xref About 80% of patients presented variable degrees of myopia, and approximately one third of the cohort had high myopia, in line with previous observations in X-linked RP xref , xref and in RPGR -related RP. xref Our data confirm a faster BCVA decline in patients with high myopia as opposed to those without high myopia. xref Moreover, the patients with typical RP and high myopia had a significantly faster progression of the disease compared to those with sine pigmento RP in the absence of high myopia."

sparser
"These experiments suggest a promising approach to treatment of RP17 that might delay the onset or possibly prevent this autosomal dominant form of RP."

sparser
"Of note, the defects of precursor mRNA (pre-mRNA) splicing, a fundamental process required by most genes before transcription, have been implicated in the autosomal dominant form of RP (adRP)."

sparser
"Despite the genetic heterogeneity of RP, a large proportion—approximately 25%—of autosomal dominant forms of RP are linked to mutations in the rhodopsin (RHO) gene."

sparser
"The autosomal dominant form of RP (adRP) can be caused by mutations in five genes and a further six loci for which the genes remain to be identified."

sparser
"Autosomal dominant forms of RP (adRP) have been linked to 12 gene loci, six of which have been cloned and sequenced (, , , , and , see )."
Proteasome affects ATXN3
| 14 2
Proteasome binds ATXN3.
| 6 2

sparser
"ATXN3 binds the proteasome and preferentially cleaves ubiquitin chains that are four or more ubiquitins in length -- remarkably, the same chain length needed to target substrates efficiently to the proteasome ( xref )."

reach
"Since ataxin-3 also associates with both the proteasome and polyubiquitin chains, it may recruit ubiquitylated substrates to the proteasome [XREF_BIBR]."

reach
"Ataxin-3 also has ubiquitin protease activity and associates with the proteasome (Burnett et al., 2003; Doss-Pepe et al., 2003; Scheel et al., 2003)."

reach
"Since ataxin-3 interacts with several E3 Ub ligases (CHIP, E4B, Hrd1, Parkin) and with the proteasome associated proteins hHR23A, hHR23B, and VCP and p97, this DUB may regulate the fate of numerous UPP substrates."

sparser
"Since ataxin-3 could also interact with proteasomes, sacsin could affect the pathogenic mechanisms of ataxin-3 dysfunctions [ xref ]."

reach
"Since ataxin-3 interacts with the proteasome and proteasomal shuttle proteins, it probably also facilitates delivery of ubiquitinated substrates to the proteasome (Box 2)."

reach
"Ataxin-3 interacts with the proteasome associated proteins Rad23A/B through UbS2."

reach
"ATXN3 binds the proteasome and preferentially cleaves ubiquitin chains that are four or more ubiquitins in length -- remarkably, the same chain length needed to target substrates efficiently to the proteasome."
Proteasome inhibits ATXN3.
| 8
| 8

reach
"Wild-type ATXN3 can be degraded by the proteasome, but whether other protein quality control pathways are involved in its turnover remains unknown."

reach
"Ataxin-3 is degraded by the proteasome."

reach
"Finally, pulse-chase labeling reveals that ataxin-3 is degraded by the proteasome, with expanded ataxin-3 being as efficiently degraded as normal ataxin-3."

reach
"However, proteasome inhibition triggered polyQ expanded ATXN3 aggregation, a process that could be linked with the global proteostasis collapse induced by this treatment."

reach
"In vitro studies revealed that inactive ataxin-3 was more slowly degraded by the proteasome and that this degradation occurred independent of ubiquitination."

reach
"For ataxin-3 to be degraded by the proteasome, it needs to come into contact with this cellular machinery."

reach
"The neuroprotective role of HSJ1 has been demonstrated in different disease models : it suppresses the aggregation of polyglutamine expanded proteins, significantly enhancing mutant huntingtin solubility in Huntington disease in cells and in mice, and promoting misfolded protein targeting to the ubiquitin-proteasome system; HSJ1a cooperates with Hsp70 to promote proteasome degradation of ataxin-3, a protein responsible for spinocerebellar ataxia type 3 (SCA3); HSJ1a prevented the aggregation of the misfolded C289G Parkin, a Parkinson disease associated ubiquitin protein ligase mutant, and restored its function in mitophagy."

reach
"p45, an ATPase subunit of the 19S proteasome, interacts with ataxin-3 in vitro and stimulates the degradation of ataxin-3 in an in vitro reconstituted degradation assay system."
ATXN3 affects RP
| 19
ATXN3 binds RP. 10 / 19
| 19

sparser
"Here we use this model to investigate two pathogenic mutations in PRPF31, SP117 and AD5, causing the autosomal dominant form of RP."

sparser
"A major cause of the autosomal dominant form of RP (ADRP) is mutation of the rhodopsin gene [1, 2] ."

sparser
"In 1981, Heckenlively et al xref presented the initial clinical description of a family believed to have an autosomal dominant form of RP."

sparser
"For patient 9, the variation predicted as pathogenic allowed the molecular diagnosis conclusion to be probably positive for the autosomal dominant form of RP."

sparser
"In this RP cohort, symptoms were first reported at 5 years of age, thus confirming the earlier onset of RPGR -RP, particularly compared to autosomal dominant RP forms. xref , xref About 80% of patients presented variable degrees of myopia, and approximately one third of the cohort had high myopia, in line with previous observations in X-linked RP xref , xref and in RPGR -related RP. xref Our data confirm a faster BCVA decline in patients with high myopia as opposed to those without high myopia. xref Moreover, the patients with typical RP and high myopia had a significantly faster progression of the disease compared to those with sine pigmento RP in the absence of high myopia."

sparser
"These experiments suggest a promising approach to treatment of RP17 that might delay the onset or possibly prevent this autosomal dominant form of RP."

sparser
"Of note, the defects of precursor mRNA (pre-mRNA) splicing, a fundamental process required by most genes before transcription, have been implicated in the autosomal dominant form of RP (adRP)."

sparser
"Despite the genetic heterogeneity of RP, a large proportion—approximately 25%—of autosomal dominant forms of RP are linked to mutations in the rhodopsin (RHO) gene."

sparser
"The autosomal dominant form of RP (adRP) can be caused by mutations in five genes and a further six loci for which the genes remain to be identified."

sparser
"Autosomal dominant forms of RP (adRP) have been linked to 12 gene loci, six of which have been cloned and sequenced (, , , , and , see )."
ATXN3 affects HDAC3
4 1 | 8 6
ATXN3 binds HDAC3.
4 | 6 6
4 | 6 3

sparser
"The interaction between Ataxin-3 and HDAC3 is thought to result in the deacetylation of histones and reduce binding of the transcription factor GATA-2 to target regions of the MMP-2 promoter."

sparser
"Interestingly, the interaction between ATXN3 and HDAC3 increases during viral infection, which promotes IFN-I-induced signaling in murine primary lung cells."

No evidence text available

No evidence text available

reach
"The interaction between Ataxin-3 and HDAC3 is thought to result in the deacetylation of histones and reduce binding of the transcription factor GATA-2 to target regions of the MMP-2 promoter."

reach
"Further, it was shown that both normal and expanded ataxin 3 physiologically interact with HDAC3 and NCoR in a SCA3 cell model and in human pons tissue; however, normal ataxin 3 containing protein complexes showed increased histone deacetylase activity, whereas polyglutamine expanded ataxin 3 containing complexes had reduced deacetylase activity in target chromatin regions [XREF_BIBR]."

reach
"Furthermore, ATXN3 physically interacts with histone deacetylase 3 (HDAC3) and upregulates the level of HDAC3 protein."

reach
"Interestingly, the effects observed in cells lacking ATXN3 are epistatic to the inhibition or lack of the histone deacetylase 3 (HDAC3), an interaction partner of ATXN3."

No evidence text available

reach
"In addition to binding and altering activity of histone acetyltransferases Atxn3 can bind to DNA and recruit histone deacetylase 3 and nuclear receptor corepressor, NCoR, to repress transcription of the matrix metalloproteinase-2 promoter."
| 3

sparser
"Both normal (Q23) and expanded (Q70) human full-length Ataxin-3 physiologically interacted with HDAC3 and NCoR in a SCA3 rat cell line and human pons tissue."

sparser
"A direct interaction of ATXN3 with HDAC3 and NCoR has previously been established in SCA3 cell lines and human brain extracts yet with only normal (i.e. not expanded) ATXN3 being associated with increased deacetylase activity and repression of gene expression [ xref ]."

sparser
"Further, it was shown that both normal and expanded ataxin 3 physiologically interact with HDAC3 and NCoR in a SCA3 cell model and in human pons tissue; however, normal ataxin 3-containing protein complexes showed increased histone deacetylase activity, whereas polyglutamine-expanded ataxin 3-containing complexes had reduced deacetylase activity in target chromatin regions [ xref ]."
ATXN3 deubiquitinates HDAC3.
1 | 2
ATXN3 deubiquitinates HDAC3. 3 / 3
1 | 2

"ATXN3 Positively Regulates Type I IFN Antiviral Response by Deubiquitinating and Stabilizing HDAC3"

reach
"Likewise, ATXN3 has been shown to exacerbate type I antiviral response via the deubiquitination and stabilization of histone deacetylase 3 (HDAC3) (Feng et al., 2018)."

reach
"Moreover, ATXN3 deubiquitinates HDAC3, thereby enhancing HDAC3 protein stability."
SCA affects ATXN3
| 2 16
SCA binds ATXN3.
| 16
| 16

sparser
"A complete genetic ataxia panel, which included autosomal dominant ataxias caused by mutations in the genes associated with SCA 1, 2, 3, 5, 6, 7, 8, 10, 12, 13, 14, 17, 28 and DRPLA, and autosomal recessive ataxias caused by mutations in genes associated with SIL1, TTPA, FRDA, POLG, APTX, and SETX was negative."

sparser
"We have studied one large French-Canadian kindred with four generations of living affected individuals segregating an autosomal dominant form of SCA."

sparser
"Optical coherence tomography (OCT) has been used as a non-invasive and easily applicable tool to evaluate structural changes of the retina and optic nerve in a wide spectrum of neurodegenerative diseases including the most common trinucleotide expansion SCAs, particularly in SCA-ATXN1 [ xref ], SCA-ATXN3 [ xref ], SCA-ATXN7 [ xref ], or a mixed SCA cohort (SCA-ATXN1,2,3 or SCA-CACNA1A) [ xref ]."

sparser
"Machado–Joseph disease (SCA-ATXN3) is the most common autosomal dominant ataxia worldwide [ xref ] and is caused by a trinucleotide (CAG) expansion located in the 10th exon of the ATXN3 gene."

sparser
"Two large kindreds with an autosomal dominant form of SCA originated from the most Northwestern part of Galicia (Spain), an area with, for many years, difficult access both by sea and by road."

sparser
"Spinocerebellar ataxia type 3 (SCA-ATXN3) is a genetic neurodegenerative disease characterized by progressive cerebellar ataxia and other variable findings, including Parkinsonian syndrome."

sparser
"NEI-VFQ revealed a significant reduction in vision-related quality of life in patients suffering from SCA-ATXN1, SCA-ATXN3, or SCA-CACNA1A. Visual impairment was not only evident regarding NEI-VFQ composite score but also in most of the subscores [ xref ]."

sparser
"There is no disease-modifying treatment for SCA-ATXN3, so symptom-based management predominates."

sparser
"We should suspect an SCA-ATXN3 etiology in patients with syndromes resembling an early-onset Parkinson disease with an autosomal dominant pattern."

sparser
"It is important to note that Pula et al. and Alvarez et al. reported a correlation between disease severity and pRNFL thinning for SCA-ATXN2 and SCA-ATXN3 or SCA-ATXN3 alone [ xref , xref ], but data were limited to small sample sizes."
SCA activates ATXN3.
| 2
SCA activates ATXN3. 2 / 2
| 2

reach
"Spinocerebellar ataxia (SCA) type 2 and 3 are caused by cytosine-adenine-guanine (CAG) n repeat expansions in ATXN2 and ATXN3 genes (OMIM 601517, 607047), which are the most common types of SCAs in China (Wang et al., 2011)."

reach
"Clinically, it is difficult to distinguish it from other autosomal dominantly inherited ataxias, and it has been suggested that MJD may be caused by an allelic variant of SCA."
PCSK9 affects ATXN3
| 18
| 16

sparser
"Gain-of-function mutations in PCSK9 are associated with autosomal dominant hypercholesterolemia ( xref , xref ), with the missense D374Y variant causing a particularly severe form of the disease ( xref )."

sparser
"Gain of function mutations in PCSK9 are associated with autosomal dominant hypercholesterolemia, a disease that is characterized by increased LDL-C levels (>300 mg/dL) and a corresponding increased ri[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Gain-of-function mutations in PCSK9 are associated with autosomal dominant hypercholesterolemia in humans."

sparser
"Gain-of-function mutations of PCSK9 gene are associated with autosomal dominant hypercholesterolemia, whereas PCSK9 deficiency leads to low LDL-C concentrations and protects against cardiovascular dis[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"PCSK9 expression is regulated by dietary cholesterol in mice and cellular sterol levels in cell culture via the sterol regulatory element binding protein transcription factors, and mutations in PCSK9 are associated with a form of autosomal dominant hypercholesterolemia."

sparser
"Though the exact mechanism of this process is not entirely known, PCSK9 inhibitors can prevent PCSK9 from degrading the LDL-Rs and significantly increase the expression of LDL-Rs, which further reduces the levels of LDL-C in the plasma. xref Mutations of PCSK9 have been reported to significantly impact cardiovascular outcomes. xref The development of gain-of-function mutations in PCSK9 is associated with autosomal dominant hypercholesterolemia, while loss-of-function mutations are thought to correlate with lower LDL-C and reduced coronary heart disease without additional deleterious effects. xref "

sparser
"Gain-of-function (GOF) PCSK9 mutations are associated to autosomal dominant hypercholesterolemia and premature atherosclerosis [ xref ]."

sparser
"Gain-of-function mutations in PCSK9 are associated with autosomal dominant hypercholesterolemia and sharply increased risk of coronary artery disease (CAD) xref , while loss-of-function mutations are accompanied by remarkably large reductions in CHD risk xref ."

sparser
"Mutations in PCSK9 are associated with an autosomal dominant form of hypercholesterolemia."

sparser
"Gain-of-function mutations in PCSK9 are associated with autosomal dominant hypercholesterolemia ( xref , xref ); conversely, loss-of-function mutations in PCSK9 are associated with lowered levels of plasma LDL-C and decreased incidence of cardiovascular heart disease ( xref , xref )."

sparser
"Pcsk9 mutations have been associated with autosomal dominant hypercholesterolemia, which is characterized by high LDL levels ( xref )."

sparser
"Population studies have shown that PCSK9 gain of function variants associate with high LDL-C levels and autosomal dominant hypercholesterolemia [ xref , xref ], whereas loss of function variants associate with low LDL-C levels [ xref , xref ]."
ATXN3 affects HSPA
| 18
ATXN3 activates HSPA.
| 13
ATXN3 activates HSPA. 10 / 13
| 13

reach
"Atxn3 increased hsp70 basal promoter activity and this may be responsible for some of the enhanced stress induced promoter activity; however, Atxn3 may modulate stress induced activity through other mechanisms."

reach
"Although Atxn3 regulated hsp70 basal promoter activity and protein, it was unexpected that Atxn3 also modulated stress induced hsp70 promoter activity and protein following heat shock and AZE stresses."

reach
"This suggested that Atxn3 modulated stress induced hsp70 promoter activity in response to these stresses."

reach
"Therefore, Atxn3 may be a general modulator of basal hsp70 in many species and tissues as well as being a potential modulator of hsp70 in response to stresses associated with excess misfolded proteins."

reach
"Atxn3 appeared to modulate hsp70 promoter activity in response to heat shock and AZE stress but not to cadmium stress."

reach
"Atxn3 responds to stress, functions in cellular proteostasis, and modulates hsp70."

reach
"As expected, 2DG treated KO cells were very similar to control cells with Atxn3 increasing basal hsp70 promoter activity and a similar increase in hsp70 promoter activity in 2DG treated cells."

reach
"We note that, despite gross similarity, SCA3 degeneration is not identical to general misfolding : some suppressors of Ataxin-3 toxicity strikingly enhanced dominant negative Hsp70 (upregulation of DnaJ-1 and Tpr2), whereas the enhancer CG11033 E3093 suppressed it."

reach
"To determine if Atxn3 modulation of hsp70 promoter activity extended beyond basal activity, WT and KO cells were treated with stressors that induce hsp70 (heat shock, cadmium, and AZE), as well as a stressor that typically does not induce hsp70, 2-DG."

reach
"Hsp70 regulation and its functions are critical for cellular homeostasis; therefore, we examined the possibility that Atxn3 modulates hsp70."
ATXN3 binds HSPA.
| 3
| 3

reach
"A similar model of CHIP and Hsp70 interaction with HTT and ATXN3 was proposed, although no single modified residue was identified as a recognition site."

reach
"This possibly reflects a more transient interaction between the E3-ligase and ataxin-3, compared to the interaction between the chaperone Hsp70 and ataxin-3."

reach
"Next, we tested whether HSJ1a, CHIP, HSP70 and Atx3 can form a complex in cells."
ATXN3 increases the amount of HSPA.
| 2
ATXN3 increases the amount of HSPA. 2 / 2
| 2

reach
"The preliminary observation that hsp70 protein appeared to be lower in Atxn3 KO fibroblasts raised the question : does Atxn3 modulate hsp70 levels and if so, is it a sufficiently robust effect to detect in mouse tissue?"

reach
"Data in the current study are consistent with Atxn3 modulating levels of hsp70 and previous studies are consistent with Atx3 regulating protein degradation suggesting that Atxn3 helps regulate two key cellular processes that protect cells against aberrant proteins."
SNCA affects ATXN3
| 17
| 17

sparser
"Currently, six missense mutations in aSyn have been associated with autosomal dominant forms of parkinsonism (A30P, E46K, H50Q, G51D, A53E, A53T) [ xref , xref , xref , xref – xref , xref ]."

sparser
"Three missense mutations (A30P, A53T and E46K) in the alpha-syn gene are associated with rare autosomal dominant forms of familial PD."

sparser
"Mutations in and alterations in expression levels of alpha-synuclein cause autosomal dominant early onset heredity forms of Parkinson's disease, and sporadic Parkinson's disease is defined in part by the presence of Lewy bodies and Lewy neurites that are composed primarily of alpha-synuclein deposited in an aggregated amyloid fibril state."

sparser
"Leucine-rich repeat kinase 2 (LRRK2) and α-synuclein are associated with autosomal dominant forms of PD, whereas parkin, PINK1 (PTEN-induced kinase 1), DJ-1 and ATP13a2 are associated with autosomal recessive forms."

sparser
"E46K) in SNCA gene have been associated with autosomal dominant parkinsonism."

sparser
"Mutations in the α-synuclein gene ( SNCA , PARK1/4 loci, OMIM 163890 ) cause rare familial forms of autosomal dominant PD [1] ."

sparser
"Mutations in the genes encoding leucine-rich repeat kinase 2 (LRRK2) and α-synuclein are associated with both autosomal dominant and idiopathic forms of Parkinson’s disease (PD)."

sparser
"Here, we describe 2 novel families in which there is autosomal dominant PD associated with SNCA duplication, and we compare the clinical features of all known patients carrying 3 or 4 SNCA copies."

sparser
"This research includes the efforts of Martikainen et al.(1) to define further the phenotype of a previously reported SNCA mutation that is associated with autosomal dominant Parkinson disease."

sparser
"Multiplications or point mutations in the gene encoding aSyn, an abundant presynaptic protein, are associated with autosomal dominant familial PD."
EMD affects ATXN3
| 17
| 17

sparser
"The autosomal dominant form of EDMD maps to LMNA , and the X-linked recessive form of EDMD is caused by loss of emerin."

sparser
"We recently identified LMNA encoding two nuclear envelope proteins, lamins A and C, to be implicated in the autosomal dominant form of EDMD."

sparser
"Wild-type lamin permits promoter release following tissue-specific activation, while a disease-linked point mutation in lamin impairs muscle-specific reorganization of a heterochromatic array during tissue-specific promoter activation in a dominant manner, which phenocopies the autosomal dominant form of EDMD [ xref ]."

sparser
"Although X-linked EDMD is associated with a mutation in the EMD gene, the autosomal dominant form of EDMD is caused by missense mutations in the LMNA gene that encodes the nuclear A-type lamins ( xref )."

sparser
"A rare autosomal dominant form of EDMD (AD-EDMD) is caused by mutations in lamin A/C gene (LMNA) on chromosome 1q21."

sparser
"Mutation in , in fact, have been demonstrated to cause the following diseases: autosomal dominant form of EDMD (), autosomal recessive form of EDMD (), limb-girdle muscular dystrophy type 1B (LGMD1B) [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Emerin ( EMD ) is among the best described Lamin-interacting proteins; it was identified as a gene mutated in patients with X-linked EDMD prior to the identification of LMNA as the gene responsible for the autosomal dominant form of EDMD (Bione et al., xref )."

sparser
"Nuclear changes are also a hallmark of the autosomal dominant form of EDMD [4,9] ."

sparser
"Following are some of such diseases: autosomal dominant form of EDMD (EDMD2, AD-EDMD; OMIM 181350) [ xref ] autosomal recessive EDMD (EDMD3, AR-EDMD; OMIM 604929), cardiomyopathy dilated 1A (CMD1A; OMIM 115200) [ xref ], limb-girdle muscular dystrophy type 1B (LGMD1B; OMIM 159001) [ xref ] congenital-type muscular dystrophy (OMIM 613205) [ xref ] and “heart-hand” syndrome (HHS; OMIM 610140) [ xref ] All mentioned diseases are caused by the mutations in LMNA gene, and have different clinical phenotypes."

sparser
"Typical features of the cardiac involvement of EDMD are presented, caused by a novel missense mutation in the splice receptor sequence of intron 6 of the LMNA gene on chromosome 1, encoding for the lamin A/C gene, consistent with the autosomal dominant form of EDMD."
ATXN3 affects SNCA
| 17
| 17

sparser
"Currently, six missense mutations in aSyn have been associated with autosomal dominant forms of parkinsonism (A30P, E46K, H50Q, G51D, A53E, A53T) [ xref , xref , xref , xref – xref , xref ]."

sparser
"Three missense mutations (A30P, A53T and E46K) in the alpha-syn gene are associated with rare autosomal dominant forms of familial PD."

sparser
"Mutations in and alterations in expression levels of alpha-synuclein cause autosomal dominant early onset heredity forms of Parkinson's disease, and sporadic Parkinson's disease is defined in part by the presence of Lewy bodies and Lewy neurites that are composed primarily of alpha-synuclein deposited in an aggregated amyloid fibril state."

sparser
"Leucine-rich repeat kinase 2 (LRRK2) and α-synuclein are associated with autosomal dominant forms of PD, whereas parkin, PINK1 (PTEN-induced kinase 1), DJ-1 and ATP13a2 are associated with autosomal recessive forms."

sparser
"E46K) in SNCA gene have been associated with autosomal dominant parkinsonism."

sparser
"Mutations in the α-synuclein gene ( SNCA , PARK1/4 loci, OMIM 163890 ) cause rare familial forms of autosomal dominant PD [1] ."

sparser
"Mutations in the genes encoding leucine-rich repeat kinase 2 (LRRK2) and α-synuclein are associated with both autosomal dominant and idiopathic forms of Parkinson’s disease (PD)."

sparser
"Here, we describe 2 novel families in which there is autosomal dominant PD associated with SNCA duplication, and we compare the clinical features of all known patients carrying 3 or 4 SNCA copies."

sparser
"This research includes the efforts of Martikainen et al.(1) to define further the phenotype of a previously reported SNCA mutation that is associated with autosomal dominant Parkinson disease."

sparser
"Multiplications or point mutations in the gene encoding aSyn, an abundant presynaptic protein, are associated with autosomal dominant familial PD."
ATXN3 affects L-glutamine
| 15 2
ATXN3 activates L-glutamine.
| 8
| 8

reach
"Spinocerebellar ataxia type 3 (SCA3) is a human polyglutamine disease caused by mutations in the gene encoding Ataxin-3, resulting in progressive dysfunction of the cerebellum [XREF_BIBR]."

reach
"SCA3 is caused by an expansion mutation of cytosine-adenine-guanine (CAG) repeats encoding a polyglutamine stretch from 51 to 91 repeats in the ataxin-3 gene, which normally has fewer than 44 CAG repeats."

reach
"We have previously shown that rapamycin attenuates the phenotype in a mouse model of Huntington disease when administered pre-symptomatically and have recently extended this to demonstrate the effectiveness of rapamycin in a transgenic mouse model of spinocerebellar ataxia type 3, a polyglutamine disorder caused by mutations in the ataxin-3 gene."

reach
"The NIs in our cases are consistently labeled for ataxin-3, yet genetic screening failed to reveal expanded CAG repeats in ataxin-3 or other diseases causing polyglutamine containing proteins."

reach
"The pathology of spinocerebellar ataxia type 3, also known as Machado-Joseph disease, is triggered by aggregation of toxic ataxin-3 (ATXN3) variants containing expanded polyglutamine repeats."

reach
"Spinocerebellar ataxia type 3 (SCA3), also known as Machado-Joseph disease, is a polyglutamine (polyQ) neurodegenerative disorder caused by abnormal (more than 40 repeats) CAG nucleotide repeat expansions in the ataxin-3 (ATXN3) gene, which encodes a protein that is involved in ubiquitin-proteasome system degradation of proteins [XREF_BIBR, XREF_BIBR]."
| PMC

reach
"Machado-Joseph disease (or spinocerebellar ataxia type 3) is a late-onset polyglutamine neurodegenerative disorder caused by a mutation in the ATXN3 gene, which encodes for the ubiquitously expressed protein ataxin-3."

reach
"SCA3 is caused by a CAG repeat expansion in the ATXN3 gene, resulting in an abnormally long polyglutamine stretch in the encoded ATXN3 protein."
ATXN3 inhibits L-glutamine.
| 7
| 7

reach
"In contrast, full-length expanded ataxin-3 with an even longer repeat caused a much milder, selectively neurotoxic phenotype, and normal ataxin-3 actually suppressed the toxicity of other polyglutamine disease proteins."

reach
"Exogenous expression of human wild-type ataxin-3 in Drosophila suppressed polyglutamine related neurodegeneration in vivo, in a manner that was dependent on its Ub binding and chain cleaving properties [XREF_BIBR]."

reach
"In Drosophila, ataxin-3 suppresses the toxicity of expanded polyglutamine proteins in a manner that requires the catalytic activity of the Josephin domain XREF_BIBR."

reach
"Ataxin-3 suppresses polyglutamine neurodegeneration in Drosophila by a ubiquitin associated mechanism."

reach
"Strikingly, it was found that ATXN3 suppresses polyglutamine neurodegeneration in Drosophila, and this suppressing activity is dependent on its interaction with ubiquitin and on its protease activity [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Like CHIP, ataxin-3 suppresses polyglutamine toxicity and does so in a manner linked to its ubiquitin associated activities."

reach
"Parkin can also target polyglutamine proteins ataxin-2 and ataxin-3 for ubiquitination to negatively control their levels and reduce these polyglutamine proteins induced cytotoxicity [XREF_BIBR, XREF_BIBR]."
| 2

sparser
"VCP also has many associations with neurodegenerative disease as a component of intranuclear and cytoplasmic aggregates ( xref ), as the mutated gene in the rare neurological disorder inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia ( xref ), and through binding to Atx-3 and other polyglutamine proteins ( xref ; xref )."

sparser
"The TERA/VCP/p97 protein also interacts with a normal length polyglutamine (polyQ) tract sequence derived from a transcription factor, Brn-2 xref , and the mutant form of a polyglutamine disease protein, ataxin-3 (ATXN3) xref , which causes spinocerebellar ataxia type 3 (SCA3)."
ATXN3 affects EMD
| 17
| 17

sparser
"The autosomal dominant form of EDMD maps to LMNA , and the X-linked recessive form of EDMD is caused by loss of emerin."

sparser
"We recently identified LMNA encoding two nuclear envelope proteins, lamins A and C, to be implicated in the autosomal dominant form of EDMD."

sparser
"Wild-type lamin permits promoter release following tissue-specific activation, while a disease-linked point mutation in lamin impairs muscle-specific reorganization of a heterochromatic array during tissue-specific promoter activation in a dominant manner, which phenocopies the autosomal dominant form of EDMD [ xref ]."

sparser
"Although X-linked EDMD is associated with a mutation in the EMD gene, the autosomal dominant form of EDMD is caused by missense mutations in the LMNA gene that encodes the nuclear A-type lamins ( xref )."

sparser
"A rare autosomal dominant form of EDMD (AD-EDMD) is caused by mutations in lamin A/C gene (LMNA) on chromosome 1q21."

sparser
"Mutation in , in fact, have been demonstrated to cause the following diseases: autosomal dominant form of EDMD (), autosomal recessive form of EDMD (), limb-girdle muscular dystrophy type 1B (LGMD1B) [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Emerin ( EMD ) is among the best described Lamin-interacting proteins; it was identified as a gene mutated in patients with X-linked EDMD prior to the identification of LMNA as the gene responsible for the autosomal dominant form of EDMD (Bione et al., xref )."

sparser
"Nuclear changes are also a hallmark of the autosomal dominant form of EDMD [4,9] ."

sparser
"Following are some of such diseases: autosomal dominant form of EDMD (EDMD2, AD-EDMD; OMIM 181350) [ xref ] autosomal recessive EDMD (EDMD3, AR-EDMD; OMIM 604929), cardiomyopathy dilated 1A (CMD1A; OMIM 115200) [ xref ], limb-girdle muscular dystrophy type 1B (LGMD1B; OMIM 159001) [ xref ] congenital-type muscular dystrophy (OMIM 613205) [ xref ] and “heart-hand” syndrome (HHS; OMIM 610140) [ xref ] All mentioned diseases are caused by the mutations in LMNA gene, and have different clinical phenotypes."

sparser
"Typical features of the cardiac involvement of EDMD are presented, caused by a novel missense mutation in the splice receptor sequence of intron 6 of the LMNA gene on chromosome 1, encoding for the lamin A/C gene, consistent with the autosomal dominant form of EDMD."
ATXN3 affects ATXN2
| 5 12
| 5 4

sparser
"Interestingly, ataxin-2 binds to SCA3, which is responsible for SCA3, another spinal cerebellar ataxia, and enhances the progression of neurodegeneration in flies."

reach
"We also analyzed the possibility of genetic interactions between ATXN1 and ATXN2, ATXN2 and ATXN3, and ATXN2 and CACNA1A."

sparser
"Protein interaction studies indicate that Atx2 and Atx3 do not interact directly; in a survey of the interaction network of ataxia-associated proteins, Atx2 and Atx3 were separated by four nodes [ xref ]."

reach
"This was of particular interest in the case of the Atx2 and Atx3 interaction, given that Atx2 had previously been identified as a modifier of Atx1."

sparser
"The Interaction between Atx2 and Pathogenic Atx3 Is Dependent on the PAM2 Motif of Atx2."

sparser
"It has been hypothesized that the polyglutamine-expanded forms of ataxin-2 (in SCA2) and ataxin-3 (in SCA3), but not the wild-type, bind to ITPR1 and potentiate mGluR1-mediated calcium release from the ER [ xref , xref ]."

reach
"In this immunohistochemical study, we showed recruitment of ataxin-2, ataxin-3 and TATA box binding protein (TBP) into NIIs of the pontine neurons of spinocerebellar ataxia type (SCA) 1, SCA2, SCA3 and dentatorubral-pallidoluysian atrophy brains."

reach
"These data are consistent with and expand upon clinical findings suggesting interactions between Atx2 and Atx3 in human disease [XREF_BIBR - XREF_BIBR]."

reach
"16 Interestingly, ataxin-2 binds to SCA3, which is responsible for SCA3, another spinal cerebellar ataxia, and enhances the progression of neurodegeneration in flies."
| 3

sparser
"Furthermore, we show that this binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA, similar to what we have shown previously for HTT (Krauss et al., xref ), induces translation in a CAG repeat length-dependent manner in vitro and in cell lines."

sparser
"To test if the flanking regions influence the binding of MID1 to CAG repeat mRNAs, we tested the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNAs."

sparser
"Furthermore, we show that functionally, in line with what we have previously observed for HTT, the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA induces protein synthesis in a repeat length-dependent manner."
ATXN2 binds HTT, ITPR1, and ATXN3. 3 / 3
| 3

sparser
"Importantly, mutant HTT, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of IP3R1 in rat neurons and increase its sensitivity to InsP3 [ xref , xref ]."

sparser
"My laboratory discovered that mutant Huntingtin, ataxin-2 and ataxin-3 proteins specifically binds to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP 3 R1), an intracellular Ca 2+ release channel."

sparser
"Our laboratory discovered that mutant huntingtin, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP(3)R1), an intracellular Ca(2+) release channel."
| 2

sparser
"In this study we show that MID1 can also bind to the CAG repeat region of ATXN2, ATXN3 , and ATXN7 in vitro , suggesting that binding of MID1 to CAG repeats is not dependent on the flanking regions."

sparser
"We show that ATXN2, ATXN3 , and ATXN7 bind to MID1 in a CAG repeat length-dependent manner."
HDAC3 affects ATXN3
4 | 6 6
4 | 6 3

sparser
"The interaction between Ataxin-3 and HDAC3 is thought to result in the deacetylation of histones and reduce binding of the transcription factor GATA-2 to target regions of the MMP-2 promoter."

sparser
"Interestingly, the interaction between ATXN3 and HDAC3 increases during viral infection, which promotes IFN-I-induced signaling in murine primary lung cells."

No evidence text available

No evidence text available

reach
"The interaction between Ataxin-3 and HDAC3 is thought to result in the deacetylation of histones and reduce binding of the transcription factor GATA-2 to target regions of the MMP-2 promoter."

reach
"Further, it was shown that both normal and expanded ataxin 3 physiologically interact with HDAC3 and NCoR in a SCA3 cell model and in human pons tissue; however, normal ataxin 3 containing protein complexes showed increased histone deacetylase activity, whereas polyglutamine expanded ataxin 3 containing complexes had reduced deacetylase activity in target chromatin regions [XREF_BIBR]."

reach
"Furthermore, ATXN3 physically interacts with histone deacetylase 3 (HDAC3) and upregulates the level of HDAC3 protein."

reach
"Interestingly, the effects observed in cells lacking ATXN3 are epistatic to the inhibition or lack of the histone deacetylase 3 (HDAC3), an interaction partner of ATXN3."

No evidence text available

reach
"In addition to binding and altering activity of histone acetyltransferases Atxn3 can bind to DNA and recruit histone deacetylase 3 and nuclear receptor corepressor, NCoR, to repress transcription of the matrix metalloproteinase-2 promoter."
| 3

sparser
"Both normal (Q23) and expanded (Q70) human full-length Ataxin-3 physiologically interacted with HDAC3 and NCoR in a SCA3 rat cell line and human pons tissue."

sparser
"A direct interaction of ATXN3 with HDAC3 and NCoR has previously been established in SCA3 cell lines and human brain extracts yet with only normal (i.e. not expanded) ATXN3 being associated with increased deacetylase activity and repression of gene expression [ xref ]."

sparser
"Further, it was shown that both normal and expanded ataxin 3 physiologically interact with HDAC3 and NCoR in a SCA3 cell model and in human pons tissue; however, normal ataxin 3-containing protein complexes showed increased histone deacetylase activity, whereas polyglutamine-expanded ataxin 3-containing complexes had reduced deacetylase activity in target chromatin regions [ xref ]."
ATXN3 affects autophagy
| 2 14
| 2 14

reach
"Paradoxically, in a fly model of SCA3 (another polyQ disease), flies expressing pathogenic SCA3 (SCA3trQ78) display increased autophagy and neurodegenerative phenotypes, indicating that increased autophagy may promote the disease."

reach
"Ataxin-3, another deubiquitinating enzyme is involved in deubiquitinating K48-linked poly-ubiquitin chains from BECN1-K402, thus stabilizing BECN1 and promoting starvation-induced autophagy (Ashkenazi et al., 2017)."

reach
"First, we determined whether normal or pathogenic Ataxin-3 itself induced lysosomal accumulation reflective of autophagy, by examining the fat body tissue from larvae, a standard assay for autophagy [XREF_BIBR]."

eidos
"Together , we conclude that ATXN3 stimulates autophagy and thereby optimizes the cellular response to nutrient starvation and proteotoxic stress , two conditions that rely heavily on autophagy ."

reach
"While Ataxin-3 deficient cells are still able to induce autophagy, the induction process appeared to be exaggerated with an increased number of autophagosomes, which is accompanied by less efficient turnover of proteins by autophagy."

reach
"No change in the number of LC3-II vesicles was observed when the Q35 tract was expressed in beclin 1 depleted cells (XREF_FIG) and the inhibitory effect of Q35 on beclin 1 levels and autophagy in beclin 1 expressing cells was rescued by ataxin-3 overexpression (XREF_FIG), compatible with the model that the Q35 acts by impairing ataxin-3 control of beclin 1 levels."

reach
"Furthermore, pathogenic ATXN3 induces autophagy in a Drosophila model."

reach
"Treatment of the larval SCA3 zebrafish with various compounds with autophagy induction capacity was able to produce the improved swimming of the zebrafish, suggesting the potential benefit of autophagy-inducing compounds for the treatment of SCA3."

reach
"The normal function of Ataxin-3 is in ubiquitin modulated pathways [XREF_BIBR - XREF_BIBR]; our data suggest the possibility that Ataxin-3 may also modulate autophagy."

reach
"According with the literature, the polyQ stretch in wild-type ataxin-3 induces autophagy by protecting Beclin-1 from proteasome-mediated degradation (Ashkenazi et al., 2017)."
| 14
| 12

reach
"ATX-3 Promotes p53 Dependent Apoptosis in Cells and in Zebrafish."

reach
"SUMOylation did not influence the subcellular localization, ubiquitination or aggregates formation of mutant-type ataxin-3, but partially increased its stability and the apoptosis rate of the cells."

reach
"Deletion of ATXN3 resulted in destabilization of p53, whereas ectopic expression of ATXN3 induced expression of p53 target genes and promoted p53-dependent apoptosis."

reach
"In addition, ATXN3 SUMOylation by SUMO-1 on site K166 also increases apoptosis in SCA3; therefore, SUMOylation by SUMO-1 might stimulate SCA3 pathogenesis through both effects described above."

reach
"As P53 has known functions in cycle arrest and apoptosis, this indicates that expression of atxn3 polyQ repeats induces selective transcription and expression of p53 target genes and promotes p53 dependent apoptosis in the CNS of zebrafish [XREF_BIBR]."
| PMC

reach
"Using the zebrafish model system, we further examined whether ATX-3 induces p53 dependent apoptosis in vivo."

reach
"These workers have also shown that overexpression of the MJD protein with expanded polyglutamine repeats leads to apoptosis, both in vitro and in vivo."

reach
"In conclusion, we demonstrated that lithium carbonate and CoQ10 reduced apoptosis induced by expanded ATX3 (Q84)."

reach
"SUMOylation did not influence the subcellular localization, ubiquitination or aggregates formation of mutant-type ataxin-3, but partially increased its stability and the cell apoptosis."

reach
"When the FL ATX-3 mRNA was injected into WT but not the p53 mutant zebrafish embryos, significantly more apoptotic cells were observed in TUNEL staining assays, indicating that ATX-3 caused p53 dependent apoptosis in vivo."
| 2

reach
"ATX-3 deletion destabilizes p53, resulting in deficiency of p53 activity and functions, whereas ectopic expression of ATX-3 induces selective transcription and expression of p53 target genes and promotes p53 dependent apoptosis in both mammalian cells and the central nervous system of zebrafish."

reach
"MicroRNA-409-3p Targeting at ATXN3 Reduces the Apoptosis of Dopamine Neurons Based on the Profile of miRNAs in the Cerebrospinal Fluid of Early Parkinson's Disease."
ATXN3 affects SCA
| 16
| 16

sparser
"A complete genetic ataxia panel, which included autosomal dominant ataxias caused by mutations in the genes associated with SCA 1, 2, 3, 5, 6, 7, 8, 10, 12, 13, 14, 17, 28 and DRPLA, and autosomal recessive ataxias caused by mutations in genes associated with SIL1, TTPA, FRDA, POLG, APTX, and SETX was negative."

sparser
"We have studied one large French-Canadian kindred with four generations of living affected individuals segregating an autosomal dominant form of SCA."

sparser
"Optical coherence tomography (OCT) has been used as a non-invasive and easily applicable tool to evaluate structural changes of the retina and optic nerve in a wide spectrum of neurodegenerative diseases including the most common trinucleotide expansion SCAs, particularly in SCA-ATXN1 [ xref ], SCA-ATXN3 [ xref ], SCA-ATXN7 [ xref ], or a mixed SCA cohort (SCA-ATXN1,2,3 or SCA-CACNA1A) [ xref ]."

sparser
"Machado–Joseph disease (SCA-ATXN3) is the most common autosomal dominant ataxia worldwide [ xref ] and is caused by a trinucleotide (CAG) expansion located in the 10th exon of the ATXN3 gene."

sparser
"Two large kindreds with an autosomal dominant form of SCA originated from the most Northwestern part of Galicia (Spain), an area with, for many years, difficult access both by sea and by road."

sparser
"Spinocerebellar ataxia type 3 (SCA-ATXN3) is a genetic neurodegenerative disease characterized by progressive cerebellar ataxia and other variable findings, including Parkinsonian syndrome."

sparser
"NEI-VFQ revealed a significant reduction in vision-related quality of life in patients suffering from SCA-ATXN1, SCA-ATXN3, or SCA-CACNA1A. Visual impairment was not only evident regarding NEI-VFQ composite score but also in most of the subscores [ xref ]."

sparser
"There is no disease-modifying treatment for SCA-ATXN3, so symptom-based management predominates."

sparser
"We should suspect an SCA-ATXN3 etiology in patients with syndromes resembling an early-onset Parkinson disease with an autosomal dominant pattern."

sparser
"It is important to note that Pula et al. and Alvarez et al. reported a correlation between disease severity and pRNFL thinning for SCA-ATXN2 and SCA-ATXN3 or SCA-ATXN3 alone [ xref , xref ], but data were limited to small sample sizes."
ATXN3 affects MDC1
2 | 8 1
ATXN3 binds MDC1.
2 | 3 1
2 | 3 1

reach
"These revealed an interaction between endogenous MDC1 and endogenous ataxin-3, which was not enhanced by DNA damage (Fig XREF_FIG A)."

No evidence text available

reach
"These findings suggest that the interaction between ataxin-3 and MDC1 is constitutive and does not require the DNA damage induced SUMOylation of MDC1."

No evidence text available

sparser
"Furthermore, the authors failed to detect the interaction between MDC1 and ATX3 observed by Pfeiffer et al. ( xref )."

reach
"In contrast, we can detect a constitutive interaction between MDC1 and ataxin-3 that is neither enhanced by DNA damage, nor dependent on the primary SUMOylation site (K1840) in MDC1 (Luo etal, 2012)."
ATXN3 activates MDC1.
| 4
ATXN3 activates MDC1. 4 / 7
| 4

reach
"Thus, while RNF4 reduces the chromatin retention of MDC1 in wild-type cells (Galanty etal, 2012), ataxin-3 has the opposite effect and increases the retention time of MDC1 on chromatin."

reach
"We also show that ataxin-3 promotes efficient MDC1 dependent DSB repair by NHEJ and HR."

reach
"As a result, Ataxin-3 promotes prolonged retention of MDC1, resulting in reduced recruitment of 53BP1 and BRCA1."

reach
"Loss of ataxin-3 markedly decreases the chromatin dwell time of MDC1 at DSBs, which can be fully reversed by co-depletion of RNF4."
ATXN3 inhibits MDC1.
| 1
ATXN3 inhibits MDC1. 1 / 2
| 1

reach
"Thus, in accordance with Pfeiffer et al, in ATX3‐inactivated cells MDC1 is prematurely released from DNA damage sites."
ATXN3 affects APP
1 | 15
1 | 11

sparser
"In the autosomal dominant forms excessive impairs mitochondrial function, and it is predicted -induced mitochondrial dysfunction initiates other AD-characteristic histopathologies."

sparser
"The amyloid precursor protein (APP) I716F mutation is associated with autosomal dominant Alzheimer's disease with the youngest age-at-onset for the APP locus."

sparser
"In transgenic mice carrying the mutant form of APP associated with autosomal dominant forms of familial AD, increased production of the Aβ42 form leads to prominent deficits in synaptic transmission[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Familial cases of AD occur as an autosomal dominant form of a mutated amyloid precursor protein (APP) gene as well as mutations in the presenilin 1 or 2 genes."

sparser
"Promoter mutations that increase the expression of APP are associated with the development of Alzheimer disease and a duplication of the APP gene locus causes autosomal dominant early-onset Alzheimer disease [ xref , xref ]."

sparser
"In contrast, familial cases of AD represent less than 2 percent of all presentations ( xref ), usually occur prior to age 55 ( xref ), and represent an autosomal dominant form of a mutated amyloid precursor protein (APP) gene as well as mutations in the presenilin 1 or 2 genes ( xref )."

sparser
"Patients with familial AD have an autosomal dominant form of a mutated amyloid precursor protein (APP) gene as well as mutations in the presenilin 1 or 2 genes ( xref )."

sparser
"It is now recognized that while expression of autosomal dominant forms of APP in mice leads to the overproduction of Aβ, amyloidopathy and to behavioral defects, it does not lead to frank neurodegeneration xref ."

No evidence text available

sparser
"Rare early-onset familial forms of Alzheimer’s disease (AD) are associated with autosomal dominant mutations in the amyloid beta precursor protein (AβPP), presenilin 1 and presenilin 2 genes (Goate and Hardy, xref )."

sparser
"Familial early-onset AD (FAD) is associated with autosomal dominant mutations in the amyloid precursor protein (APP) and in the catalytic subunits (presenilin 1 and presenilin 2) of the intramembrane protease that processes it, γ-secretase ( xref )."

sparser
"Early-onset autosomal dominant AD genes are associated with excessive accumulation, however cognitive impairment best correlates with NFTs and disrupted microtubules."
APP binds ATXN3 and AD. 2 / 2
| 2

sparser
"There are only two dozen families carrying autosomal dominant APP mutations associated with early-onset AD."

sparser
"Among the early-onset AD cases, few carry a rare familial form of AD with an inheritable autosomal dominant mutation in either amyloid precursor protein (APP), presenilin-1 or presenilin-2 genes."
ATXN3 affects AD
| 16
ATXN3 binds AD. 10 / 14
| 14

sparser
"Indeed, some, but not all, investigators have considered a PSEN1 mutation in Auguste Deter, Alzheimer’s original patient. xref In actuality, the vast majority of patients with early-onset AD have a nonfamilial, or sporadic, form. xref Only approximately 11% of those with early-onset AD (about 0.6% of the total of all patients with AD, including the late-onset form) have familial AD associated with one of the three known autosomal dominant mutations: amyloid precursor protein ( APP ), presenilin 1 ( PSEN1 ), or presenilin 2 ( PSEN2 ). xref Studies screening for these genes in patients with early-onset AD report a prevalence of 0.8% for APP, 1.1% for PSEN1, and as high as 13% for potential pathogenic variants of PSEN2, which can present even after age 65. xref – xref These forms of familial AD are usually inherited from a parent with a similar age of onset of early-onset AD, generally in the forties or fifties (having a positive family history of late-onset AD has no effect). xref These three pathogenic mutations, which lead to aberrant cleavage or aggregation of the amyloid precursor protein, result in the more typical amnestic AD but can have diagnostic features such as spastic paraparesis, early myoclonus, seizures, dysarthria, pseudobulbar affect, more extensive amyloid angiopathy, and atypical amyloid plaque morphology and distribution. xref Some PSEN1 mutations (such as A79V) can be variable and sometimes mild, with ages of onset ranging from 53 to 84. xref "

sparser
"However, more than 200 different mutations have been identified in 3 genes associated with an autosomal dominant form of AD, referred to as early-onset familial Alzheimer disease (EOFAD)."

sparser
"Three genes with causal mutations for rare, early onset (before 65 years of age) autosomal dominant forms of AD have been identified: amyloid precursor protein (; MIM# 104760l , presenilin 1 (; MIM# 1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"The complex is composed of presenilin (PS1 or PS2), anterior pharynx defect-1(APH-1), nicastrin (NCT) and PEN-2 and early-onset, autosomal dominant forms of AD are caused by inheritance of mutations of PS."

sparser
"Linkage studies have uncovered mutations in certain genes causing autosomal dominant forms of AD (ADAD), including presenilin ( PSEN1, PSEN2 ), amyloid precursor protein ( APP ), and a moderately penetrant polymorphism, APOE-e4 (for AD genetics overview, see ( xref ))."

sparser
"Early-onset also called familial AD (FAD) is a rare autosomal dominant inheritance form of AD, affecting around 5% of all people who have AD [36] and may be linked to tau or to genetic variations [[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Early onset familial AD (FAD) is a rare, fully penetrant, autosomal dominant form of AD [ xref ] due to mutations in the PSEN1 , APP , or PSEN2 genes."

sparser
"People with the much less common autosomal dominant form of AD (ADAD xref ) typically develop symptoms at an early age, generally before age 60 and as early as the third decade of life (e.g., xref )."

sparser
"Patients with an autosomal dominant familial form of early-onset AD not only have an increased risk for the development of AD among relatives, but may also have atypical clinical features, including headaches, myoclonus, seizures, gait abnormalities, pseudobulbar palsy, or hyperreflexia. xref , xref Second, overall deterioration is faster in early-onset AD as compared to late-onset AD (not related to APOE ). xref Several studies indicate that patients with early-onset AD have a potentially more aggressive clinical course. xref – xref After controlling for the direct effects of aging on mortality, patients with early-onset AD are at a greater risk for mortality compared to those with late-onset AD, xref and early-onset AD accounts for a large number of premature deaths among those 40 to 64 years of age. xref Third, patients with early-onset AD have a longer duration of the disease before diagnosis (about 1.6 years), xref , xref probably reflecting missed or delayed diagnosis or a greater extent of evaluation for diagnosis. xref Fourth, patients with early-onset AD are more likely to have a history of traumatic brain injury as a risk factor for dementia. xref However, patients with early-onset AD have decreased cerebrovascular risk factors, circulatory problems, diabetes mellitus, and obesity than those with late-onset AD. xref , xref , xref "

sparser
"Autosomal dominant forms of AD are very rare (prevalence <1%) and mostly present as EOAD."
APP binds ATXN3 and AD. 2 / 2
| 2

sparser
"There are only two dozen families carrying autosomal dominant APP mutations associated with early-onset AD."

sparser
"Among the early-onset AD cases, few carry a rare familial form of AD with an inheritable autosomal dominant mutation in either amyloid precursor protein (APP), presenilin-1 or presenilin-2 genes."
APP affects ATXN3
1 | 15
1 | 11

sparser
"In the autosomal dominant forms excessive impairs mitochondrial function, and it is predicted -induced mitochondrial dysfunction initiates other AD-characteristic histopathologies."

sparser
"The amyloid precursor protein (APP) I716F mutation is associated with autosomal dominant Alzheimer's disease with the youngest age-at-onset for the APP locus."

sparser
"In transgenic mice carrying the mutant form of APP associated with autosomal dominant forms of familial AD, increased production of the Aβ42 form leads to prominent deficits in synaptic transmission[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Familial cases of AD occur as an autosomal dominant form of a mutated amyloid precursor protein (APP) gene as well as mutations in the presenilin 1 or 2 genes."

sparser
"Promoter mutations that increase the expression of APP are associated with the development of Alzheimer disease and a duplication of the APP gene locus causes autosomal dominant early-onset Alzheimer disease [ xref , xref ]."

sparser
"In contrast, familial cases of AD represent less than 2 percent of all presentations ( xref ), usually occur prior to age 55 ( xref ), and represent an autosomal dominant form of a mutated amyloid precursor protein (APP) gene as well as mutations in the presenilin 1 or 2 genes ( xref )."

sparser
"Patients with familial AD have an autosomal dominant form of a mutated amyloid precursor protein (APP) gene as well as mutations in the presenilin 1 or 2 genes ( xref )."

sparser
"It is now recognized that while expression of autosomal dominant forms of APP in mice leads to the overproduction of Aβ, amyloidopathy and to behavioral defects, it does not lead to frank neurodegeneration xref ."

No evidence text available

sparser
"Rare early-onset familial forms of Alzheimer’s disease (AD) are associated with autosomal dominant mutations in the amyloid beta precursor protein (AβPP), presenilin 1 and presenilin 2 genes (Goate and Hardy, xref )."

sparser
"Familial early-onset AD (FAD) is associated with autosomal dominant mutations in the amyloid precursor protein (APP) and in the catalytic subunits (presenilin 1 and presenilin 2) of the intramembrane protease that processes it, γ-secretase ( xref )."

sparser
"Early-onset autosomal dominant AD genes are associated with excessive accumulation, however cognitive impairment best correlates with NFTs and disrupted microtubules."
APP binds ATXN3 and AD. 2 / 2
| 2

sparser
"There are only two dozen families carrying autosomal dominant APP mutations associated with early-onset AD."

sparser
"Among the early-onset AD cases, few carry a rare familial form of AD with an inheritable autosomal dominant mutation in either amyloid precursor protein (APP), presenilin-1 or presenilin-2 genes."
AD affects ATXN3
| 16
ATXN3 binds AD. 10 / 14
| 14

sparser
"Indeed, some, but not all, investigators have considered a PSEN1 mutation in Auguste Deter, Alzheimer’s original patient. xref In actuality, the vast majority of patients with early-onset AD have a nonfamilial, or sporadic, form. xref Only approximately 11% of those with early-onset AD (about 0.6% of the total of all patients with AD, including the late-onset form) have familial AD associated with one of the three known autosomal dominant mutations: amyloid precursor protein ( APP ), presenilin 1 ( PSEN1 ), or presenilin 2 ( PSEN2 ). xref Studies screening for these genes in patients with early-onset AD report a prevalence of 0.8% for APP, 1.1% for PSEN1, and as high as 13% for potential pathogenic variants of PSEN2, which can present even after age 65. xref – xref These forms of familial AD are usually inherited from a parent with a similar age of onset of early-onset AD, generally in the forties or fifties (having a positive family history of late-onset AD has no effect). xref These three pathogenic mutations, which lead to aberrant cleavage or aggregation of the amyloid precursor protein, result in the more typical amnestic AD but can have diagnostic features such as spastic paraparesis, early myoclonus, seizures, dysarthria, pseudobulbar affect, more extensive amyloid angiopathy, and atypical amyloid plaque morphology and distribution. xref Some PSEN1 mutations (such as A79V) can be variable and sometimes mild, with ages of onset ranging from 53 to 84. xref "

sparser
"However, more than 200 different mutations have been identified in 3 genes associated with an autosomal dominant form of AD, referred to as early-onset familial Alzheimer disease (EOFAD)."

sparser
"Three genes with causal mutations for rare, early onset (before 65 years of age) autosomal dominant forms of AD have been identified: amyloid precursor protein (; MIM# 104760l , presenilin 1 (; MIM# 1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"The complex is composed of presenilin (PS1 or PS2), anterior pharynx defect-1(APH-1), nicastrin (NCT) and PEN-2 and early-onset, autosomal dominant forms of AD are caused by inheritance of mutations of PS."

sparser
"Linkage studies have uncovered mutations in certain genes causing autosomal dominant forms of AD (ADAD), including presenilin ( PSEN1, PSEN2 ), amyloid precursor protein ( APP ), and a moderately penetrant polymorphism, APOE-e4 (for AD genetics overview, see ( xref ))."

sparser
"Early-onset also called familial AD (FAD) is a rare autosomal dominant inheritance form of AD, affecting around 5% of all people who have AD [36] and may be linked to tau or to genetic variations [[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Early onset familial AD (FAD) is a rare, fully penetrant, autosomal dominant form of AD [ xref ] due to mutations in the PSEN1 , APP , or PSEN2 genes."

sparser
"People with the much less common autosomal dominant form of AD (ADAD xref ) typically develop symptoms at an early age, generally before age 60 and as early as the third decade of life (e.g., xref )."

sparser
"Patients with an autosomal dominant familial form of early-onset AD not only have an increased risk for the development of AD among relatives, but may also have atypical clinical features, including headaches, myoclonus, seizures, gait abnormalities, pseudobulbar palsy, or hyperreflexia. xref , xref Second, overall deterioration is faster in early-onset AD as compared to late-onset AD (not related to APOE ). xref Several studies indicate that patients with early-onset AD have a potentially more aggressive clinical course. xref – xref After controlling for the direct effects of aging on mortality, patients with early-onset AD are at a greater risk for mortality compared to those with late-onset AD, xref and early-onset AD accounts for a large number of premature deaths among those 40 to 64 years of age. xref Third, patients with early-onset AD have a longer duration of the disease before diagnosis (about 1.6 years), xref , xref probably reflecting missed or delayed diagnosis or a greater extent of evaluation for diagnosis. xref Fourth, patients with early-onset AD are more likely to have a history of traumatic brain injury as a risk factor for dementia. xref However, patients with early-onset AD have decreased cerebrovascular risk factors, circulatory problems, diabetes mellitus, and obesity than those with late-onset AD. xref , xref , xref "

sparser
"Autosomal dominant forms of AD are very rare (prevalence <1%) and mostly present as EOAD."
APP binds ATXN3 and AD. 2 / 2
| 2

sparser
"There are only two dozen families carrying autosomal dominant APP mutations associated with early-onset AD."

sparser
"Among the early-onset AD cases, few carry a rare familial form of AD with an inheritable autosomal dominant mutation in either amyloid precursor protein (APP), presenilin-1 or presenilin-2 genes."
| 15

sparser
"Atlastin ( SPG3A ) is a binding partner of spastin whose mutations lead to an autosomal dominant form of HSP similar to that found with SPAST mutations, although with childhood onset ( xref ; xref )."

sparser
"The original identification of atlastin1/SPG3A occurred through genetic analysis of three independent kindreds (autosomal dominant [AD] HSP-P, -T, and -S), each presenting with an early-onset autosomal dominant form of HSP ( xref )."

sparser
"In 15–40% of families with the 'pure' autosomal dominant form of HSP, DNA sequence analysis identified a mutation in the SPG4 gene (spastin) [ xref ] and in ~10% a mutation in the SPG3A gene (atlastin) [ xref ]."

sparser
"The most common autosomal dominant (AD) forms of HSP are SPG4 (SPAST gene) and SPG3 (ATL1 gene)."

sparser
"Mutations in the REEP1 gene appear as the third most frequent cause in families with the 'pure' autosomal dominant form of HSP."

sparser
"Besides the more common autosomal dominant forms of HSP, there is a long list of recessive mutations that can lead to complex forms of HSP."

sparser
"Mutations in the non-imprinted Prader-Willi/Angelman syndrome locus 1 (NIPA1) transmembrane protein cause an autosomal dominant form of HSP (SPG6)."

sparser
"Autosomal dominant (AD) forms of HSP are mainly pure forms with ages at onset that can range from infancy to late adulthood."

sparser
"Therefore mutation analysis in the REEP1 gene should be included in the comprehensive care for patients with the 'pure' autosomal dominant form of HSP."

sparser
"Spastic paraplegia type 10 (OMIM 604187 ) is an autosomal dominant form of HSP caused by mutations in the KIF5A gene that encodes a kinesin heavy chain protein (KHC) involved in the anterograde tra[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
ATXN3 affects RNF8
| 12 2
ATXN3 binds RNF8.
| 6 2
| 6 2

sparser
"Since we confirmed an interaction between ATX3 and RNF8 in vivo (Fig  xref ), we analysed whether ATX3 regulates RNF8 homeostasis."

reach
"Since we confirmed an interaction between ATX3 and RNF8 in vivo (Fig 3), we analysed whether ATX3 regulates RNF8 homeostasis."

sparser
"However, under our experimental conditions, we found that ATX3 forms a complex with RNF8 but not MDC1."

reach
"However, under our experimental conditions, we found that ATX3 forms a complex with RNF8 but not MDC1."

reach
"Our results reveal a mechanism to explain how the p97–ATX3RNF8 complex fine‐tunes DSB repair by preventing excessive RNF8‐dependent K63‐Ub modifications and 5′‐DNA end resection and consequently promoting the NHEJ pathway, an essential pathway for cell survival after IR and thus cancer cell resistance to radiotherapy (Jeggo et al, 2011; Jeggo & Lobrich, 2015)."

reach
"However, further studies are required to address this important question in the context of the p97–ATX3RNF8 complex and chromatin dynamics after DSB formation.While our manuscript was in preparation, Pfeiffer et al (2017) reported that ATX3 is a DUB acting at DSB sites and removes ubiquitinated chains from MDC1 to prevent a premature removal of MDC1 from the breaks."

reach
"To elucidate mechanistic insight of the p97–ATX3RNF8 complex in DSB repair, we focused on sites of DNA lesions induced by either IR or UV‐laser micro‐irradiation."

reach
"Overall, these results suggest that RNF8 forms a complex with the p97 system composed of p97, Npl4–Ufd1 and ATX3 and that p97 and ATX3 bind RNF8 independently (Fig 3H)."
ATXN3 inhibits RNF8.
| 2
ATXN3 inhibits RNF8. 2 / 2
| 2

reach
"However, inactivation of p97 by chemical inhibition or siRNA‐mediated ATX3 depletion again strongly induced RNF8 accumulation at the sites of IR or two‐photon micro‐irradiation DNA lesions in fixed or living cells, respectively (Fig EV2K and L)."

reach
"Surprisingly, depletion of ATX3 with three different siRNAs also caused RNF8 and K63‐Ub hyper‐accumulation although to a lesser extent than in p97‐depleted cells."
ATXN3 deubiquitinates RNF8.
| 2
ATXN3 deubiquitinates RNF8. 2 / 2
| 2

reach
"ATX3 deubiquitinates RNF8 to protect it from premature degradation."

reach
"To directly prove that ATX3 deubiquitinates RNF8, we co‐expressed Flag‐RNF8 in either HeLa‐WT or ATX3‐knockout cells together with GFP‐ATX3‐WT, catalytically inactive GFP‐ATX3‐C14A or ubiquitin‐binding‐defective interacting motifs (UIMs*) GFP‐ATX3‐UIM* variants."
ATXN3 activates RNF8.
| 2
ATXN3 activates RNF8. 2 / 2
| 2

reach
"In this scenario, ATX3 promotes sufficient p97‐dependent extraction of RNF8 in a classical ERAD‐like manner (Wang et al, 2006; Zhong & Pittman, 2006)."

reach
"Moreover, ATX3‐knockout cells displayed delayed clearance of both MDC1 and γ‐H2AX from DSB sites, suggesting defective DSB repair.Overall, we concluded that ATX3 inactivation causes retention and consequently increased accumulation of RNF8 at DSB sites."
ATXN3 affects Proteasome
| 7 2
ATXN3 binds Proteasome.
| 6 2

sparser
"ATXN3 binds the proteasome and preferentially cleaves ubiquitin chains that are four or more ubiquitins in length -- remarkably, the same chain length needed to target substrates efficiently to the proteasome ( xref )."

reach
"Since ataxin-3 also associates with both the proteasome and polyubiquitin chains, it may recruit ubiquitylated substrates to the proteasome [XREF_BIBR]."

reach
"Ataxin-3 also has ubiquitin protease activity and associates with the proteasome (Burnett et al., 2003; Doss-Pepe et al., 2003; Scheel et al., 2003)."

reach
"Since ataxin-3 interacts with several E3 Ub ligases (CHIP, E4B, Hrd1, Parkin) and with the proteasome associated proteins hHR23A, hHR23B, and VCP and p97, this DUB may regulate the fate of numerous UPP substrates."

sparser
"Since ataxin-3 could also interact with proteasomes, sacsin could affect the pathogenic mechanisms of ataxin-3 dysfunctions [ xref ]."

reach
"Since ataxin-3 interacts with the proteasome and proteasomal shuttle proteins, it probably also facilitates delivery of ubiquitinated substrates to the proteasome (Box 2)."

reach
"Ataxin-3 interacts with the proteasome associated proteins Rad23A/B through UbS2."

reach
"ATXN3 binds the proteasome and preferentially cleaves ubiquitin chains that are four or more ubiquitins in length -- remarkably, the same chain length needed to target substrates efficiently to the proteasome."
ATXN3 activates Proteasome.
| 1
| 1

reach
"Whether CHIP is the primary mediator of ubiquitin dependent degradation of ataxin-3 is controversial : XREF_BIBR reported that CHIP ubiquitinates ataxin-3 and directs it to the proteasome for degradation, whereas XREF_BIBR reported that another ubiquitin ligase, E4B, mediates ataxin-3 degradation while CHIP has no effect."
RAD23B affects ATXN3
5 2 | 3 3
5 2 | 3 1

No evidence text available

No evidence text available

No evidence text available

No evidence text available

reach
"HR23B can directly bind the proteasome and ataxin-3, a deubiquitinase enzyme that binds ubiquitinated proteins and can also bind to the proteasome [XREF_BIBR, XREF_BIBR]."

reach
"It has been reported that ATX3 interacts with HHR23A and HHR23B, homolog of DNA repair protein RAD23, and that ATX3 can be recruited to the DNA damage sites induced by laser microirradiation."

No evidence text available

sparser
"Essential information on Fig. 14 Exemplary interaction between the two human proteins HHR23B and ataxin-3."
| PMC

No evidence text available

No evidence text available
| 2

sparser
"It has been reported that ATX3 interacts with HHR23A and HHR23B, homolog of DNA repair protein RAD23 ( xref ), and that ATX3 can be recruited to the DNA damage sites induced by laser microirradiation ( xref )."

sparser
"Initial studies found that ataxin-3 interacts with two proteins, HHR23A and HHR23B, that are both homologs of the DNA repair protein Rad23 [ xref ]."

reach
"50 Interestingly, ATXN3 has been reported to repress transcription through recruitment of HDAC3 to target promoters 38 or by inhibiting a histone acetyltransferase."

reach
"Notably, ATXN3 depletion significantly decreased global transcription, repair of transcribed genes, and error-free double-strand break repair of a 3 '-phosphate-containing terminally gapped, linearized reporter plasmid."

eidos
"The lack of ATXN3 leads to abnormalities in nuclear and nucleolar morphology , alters DNA replication timing and increases transcription ."

reach
"Similar to the polyQ-expansions of sizes> = 34 in the polysome associated protein ATXN2 causing Spinocerebellar Ataxia Type 2 (SCA2), polyQ expansions of sizes> = 52 in the deubiquitinating transcript[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Ataxin-3 is thought to repress transcription via histone dependent chromatin remodeling [XREF_BIBR, XREF_BIBR], and huntingtin modulates the expression of NRSE controlled genes [XREF_BIBR]."

reach
"Since expanded ATX3 may disrupt normal gene transcription patterns, lithium may act as a protective factor by modulating gene expression."

reach
"ATX-3 deletion destabilizes p53, resulting in deficiency of p53 activity and functions, whereas ectopic expression of ATX-3 induces selective transcription and expression of p53 target genes and promotes p53 dependent apoptosis in both mammalian cells and the central nervous system of zebrafish."

reach
"In the present study we use cell transfections, in vitro binding, co-immunoprecipitations, and reporter assays to show that the polyglutamine disease protein, ataxin-3, interacts with the major histone acetyltransferases cAMP-response-element binding protein (CREB)-binding protein, p300, and p300 and CREB binding protein associated factor and inhibits transcription by these coactivators."

reach
"Additionally, ataxin-3 also binds coactivators like CBP, p300, and CBP and p300 associated factor (PCAF) and represses the respective coactivator mediated transcription."

reach
"Furthermore, microarray gene expression profiling in MJD and SCA3 transgenic mice revealed that expanded ATX3 may cause cerebellar dysfunction and ataxia by disrupting the normal pattern of gene transcription."

reach
"For example, ATXN3 has been shown to modulate transcription factor and repressor degradation by directly altering ubiquitin chains attached to such proteins [XREF_BIBR, XREF_BIBR]."

reach
"The lack of ATXN3 leads to abnormalities in nuclear and nucleolar morphology, alters DNA replication timing and increases transcription."

reach
"As P53 has known functions in cycle arrest and apoptosis, this indicates that expression of atxn3 polyQ repeats induces selective transcription and expression of p53 target genes and promotes p53 dependent apoptosis in the CNS of zebrafish [XREF_BIBR]."
| PMC
ATXN3 affects RAD23B
5 2 | 3 3
5 2 | 3 1

No evidence text available

No evidence text available

No evidence text available

No evidence text available

reach
"HR23B can directly bind the proteasome and ataxin-3, a deubiquitinase enzyme that binds ubiquitinated proteins and can also bind to the proteasome [XREF_BIBR, XREF_BIBR]."

reach
"It has been reported that ATX3 interacts with HHR23A and HHR23B, homolog of DNA repair protein RAD23, and that ATX3 can be recruited to the DNA damage sites induced by laser microirradiation."

No evidence text available

sparser
"Essential information on Fig. 14 Exemplary interaction between the two human proteins HHR23B and ataxin-3."
| PMC

No evidence text available

No evidence text available
| 2

sparser
"It has been reported that ATX3 interacts with HHR23A and HHR23B, homolog of DNA repair protein RAD23 ( xref ), and that ATX3 can be recruited to the DNA damage sites induced by laser microirradiation ( xref )."

sparser
"Initial studies found that ataxin-3 interacts with two proteins, HHR23A and HHR23B, that are both homologs of the DNA repair protein Rad23 [ xref ]."
ATXN3 affects PTEN
| 6 4
ATXN3 binds PTEN.
| 4
| 4

sparser
"PTEN hamartoma tumour syndrome (PHTS) is a heterogeneous group of rare, autosomal dominant disorders associated with PTEN germline mutations."

sparser
"Human germline mutations in PTEN are associated with the rare autosomal dominant hamartomatous syndromes, Cowden Disease and Bannayan–Riley–Ruvalcaba syndrome, the former including an increased risk[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Germline mutation of PTEN is associated with rare autosomal dominant cancer syndromes such as Cowden disease and Lhermitte-Duclos disease, and leads to an increased susceptibility to cancer ( xref ; xref ; xref ; xref ; xref )."

sparser
"Considering the significance of PTEN as a tumor suppressor gene and that germline mutations in PTEN are associated with several autosomal dominant hamartoma syndromes [20] including Cowden's disease[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
ATXN3 inhibits PTEN.
| 1
ATXN3 inhibits PTEN. 1 / 3
| 1

reach
"Ataxin-3 promotes testicular cancer cell proliferation by inhibiting anti-oncogene PTEN."
ATXN3 decreases the amount of PTEN.
| 3
ATXN3 decreases the amount of PTEN. 3 / 3
| 3

reach
"In addition, up-regulation of Ataxin-3 inhibited the expression of PTEN and activated the AKT and mTOR pathway."

reach
"The deubiquitylase Ataxin-3 restricts PTEN transcription in lung cancer cells."

reach
"Moreover, a recent study by Coulson and colleagues 46 provided convincing evidence that ataxin-3 restricts the transcription of the tumor suppressor PTEN."
ATXN3 activates PTEN.
| 2
ATXN3 activates PTEN. 2 / 3
| 2

reach
"In contrast, the two individual ATXN3 siRNAs (siATXN3_3 and _ 5) that caused the most profound PTEN induction (XREF_FIG and XREF_FIG) blunted Akt T308 phosphorylation in response to EGF (XREF_FIG)."

reach
"Therefore, ATXN3 may directly regulate the PTEN transcript, or indirectly affect it through regulation of a ceRNA such as PTENP1."
ATXN3 affects HTT
| 13
| 8

sparser
"In previous studies, we demonstrated that polyQ-expanded Huntingtin (Htt exp ) and ataxin-3 (Atx3 exp ) specifically bound to the InsP 3 R1 carboxy-terminal region ( xref ; xref )."

sparser
"The best studied family member, UBQLN1, also known as protein linking integrin-associated protein and cytoskeleton-1 (PLIC-1), is reported to interact with the polyQ disease proteins huntingtin (HTT) and ataxin-3 (ATXN3) ( xref ; xref )."

sparser
"Mutant HTT and ataxin-3 proteins form interactions that are difficult to disrupt using traditional small molecule drugs xref ."

sparser
"Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder that is associated with mutations in the huntingtin gene (htt) [ xref ]."

sparser
"Since DAPI shows modest selectivity and reasonable binding affinity for an RNA containing a single copy of the 5’C A G/3’G A C motif that is associated with HD and SCA3, we aimed to identify chemically similar compounds with improved affinities and selectivities."

sparser
"A recent report demonstrated that CHIP, a co-chaperone of Hsc70 and ubiquitin ligase for misfolded proteins, selectively binds to polyQ-expanded ataxin-3 or huntingtin but not to the normal proteins ([MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"The best studied family member, UBQLN1, also known as protein linking integrin-associated protein and cytoskeleton-1 (PLIC-1), is reported to interact with the polyQ disease proteins Huntingtin (HTT) [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"For instance, when mutant forms of either huntingtin (Huntington’s disease) or MJD1 (spinocerebellar ataxia type 3) were directed to the fly eye, each provoked late-onset degeneration preferential[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
ATXN2 binds HTT, ITPR1, and ATXN3. 3 / 3
| 3

sparser
"Importantly, mutant HTT, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of IP3R1 in rat neurons and increase its sensitivity to InsP3 [ xref , xref ]."

sparser
"My laboratory discovered that mutant Huntingtin, ataxin-2 and ataxin-3 proteins specifically binds to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP 3 R1), an intracellular Ca 2+ release channel."

sparser
"Our laboratory discovered that mutant huntingtin, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP(3)R1), an intracellular Ca(2+) release channel."
HTT binds ATXN3 and POLR2A. 2 / 2
| 2

sparser
"PNKP has been shown to promote transcription-coupled double-strand break repair ( xref ) and often performs transcription-coupled repair when in complex with huntingtin protein, RNA polymerase II subunit A, ataxin-3, and cyclic AMP-response element binding protein (CBP) ( xref )."

sparser
"The mechanisms for this were recently suggested to be through direct interactions between HTT and RNA polymerase II subunit A (POLR2A), ataxin-3, the DNA repair enzyme polynucleotide-kinase-3'-phosphatase (PNKP), and cyclic AMP-response element-binding (CREB) protein (CBP) [ xref ], as well as complexes with the DNA double-strand break repair complex Ku70/Ku80 [ xref ]."
Sch1 affects ATXN3
| 12
| 12

sparser
"Mutations in FGFR1 cause autosomal dominant forms of KS [ xref ]."

sparser
"KAL2, identified as the fibroblast growth factor receptor 1 (FGFR1) gene, has now been recognised to be the underlying genetic defect for an autosomal dominant form of KS."

sparser
"Rare variants in FGFR1 were first thought to cause autosomal dominant form of KS ( xref )."

sparser
"In 2003, Fibroblast growth factor receptor 1 ( FGFR1) was identified as the first gene causing the autosomal dominant form of KS by mapping overlapping deletions on chromosome 8p11-p12 in two KS patients with contiguous gene syndromes ( xref )."

sparser
"Loss-of-function mutations in FGFR1 have also been associated with an autosomal dominant form of KS (KAL2); however this form of the disease is incompletely penetrant, indicating a more complex and probably oligogenic basis [ xref ; xref ]."

sparser
"Among them, FGFR1 is undoubtedly the most studied because mutations in FGFR1 gene ( KAL2 ) are responsible for the autosomal dominant form of KS [ xref ]."

sparser
"Various loss-of-function mutations in KAL1 , encoding the extracellular matrix glycoprotein anosmin-1, and in FGFR1 or FGF8 , encoding fibroblast growth factor receptor-1 and fibroblast growth factor-8, underlie the X-chromosome linked form and an autosomal dominant form of KS, respectively."

sparser
"Both FGFR1 and FGF8 , mapping on 8p12 and 10q24, respectively, can cause an autosomal dominant form of KS."

sparser
"KAL-2 is the gene responsible for an autosomal dominant form of KS and encodes the fibroblast growth factor type one receptor (FGFR1) [ xref ]."

sparser
"After the discovery that FGFR1 is responsible for an autosomal dominant form of KS, it was hypothesized that anosmin-1 could interact with FGFR to promote the binding of the FGF ligand [ xref ]."
HDAC6 affects ATXN3
1 | 5 4
1 | 5 4

reach
"Interestingly, ataxin 3 also binds HDAC6."

sparser
"Interestingly, ataxin 3 also binds HDAC6 ( xref )."

sparser
"Formation of aggresomes requires the activity of the deubiquitinating enzyme ataxin-3 which can bind to HDAC6 and trims both K48- and K63-linked ubiquitin chains [ xref , xref ]."

No evidence text available

sparser
"Collectively, our results suggest a novel mechanism of ataxin-3-mediated HDAC6-dependent aggresome formation, where the aggregate-specific deubiquitinase, ataxin-3, cleaves (poly)ubiquitin moieties in polyubiquitinated proteins to expose their C-terminal diglycine motifs, providing a handle for HDAC6 binding and subsequent transport to the aggresome."

reach
"Formation of aggresomes requires the activity of the deubiquitinating enzyme ataxin-3 which can bind to HDAC6 and trims both K48- and K63 linked ubiquitin chains [XREF_BIBR, XREF_BIBR]."

reach
"These results indicated the functional relationship of VCP with both ERAD (Hsp40 and Hsp70 complex) and aggresome formation (Ataxin3 and HDAC6 complex) pathways [XREF_BIBR]."

sparser
"Indeed, in ataxin 3-deficient cells, HDAC6 no longer associates with ubiquitinated protein aggregates ( xref )."

reach
"These results indicate the functional relationship of VCP with protein folding (Hsp40-Hsp70 and Hsp90 complex), ERAD (gp78 complex) and aggresome formation (Ataxin3 and HDAC6 complex, our unpublished data) pathways [XREF_BIBR]."

reach
"In addition, ataxin-3 interacts with dynein and the cytosolic histone deacetylase 6 during transport of misfolded proteins in cultured cells, thus linking ataxin-3 to cytoskeletal transport processes."
| 4 8
| 7

sparser
"The interaction between ataxin-3 and these E3s may serve several purposes."

sparser
"In addition to CHIP, Ataxin-3 also interacts with another E3 ligase, which is parkin ( xref ; xref )."

sparser
"Ataxin-3 interacts with two E3 ligases that are essential for maintaining normal cellular hemostasis, namely C terminus of Hsc70-interacting protein (CHIP) and parkin."

sparser
"Interestingly, ataxin-3 associates with a putative ubiquitin ligase, the potassium channel tetramerization domain-10 (KCTD10) protein ( xref )."

sparser
"Early studies hinted that the U -box E3-Ub ligase CHIP might be one such E3 that interacts with ataxin-3."

sparser
"The regulation of ataxin-3 activity through ubiquitination might depend on the interaction of ataxin-3 with several E3 ubiquitin ligases (Durcan and Fon, xref ), such as the C-terminus of 70 kDa heat-shock protein (Hsp70)-interacting protein (CHIP), parkin, and E4B (Figure xref ), since all were shown to promote ataxin-3 ubiquitination and regulate its degradation by the proteasome (Matsumoto et al., xref ; Jana et al., xref ; Miller et al., xref )."

sparser
"After all, there are reports of ubiquitin ligases that interact with ataxin-3, increasing its ubiquitination and its proteasomal turnover ( xref ; xref ; xref ; xref ; xref )."
E3_Ub_ligase ubiquitinates ATXN3.
| 4 1
E3_Ub_ligase ubiquitinates ATXN3. 5 / 5
| 4 1

reach
"Several E3 ubiquitin ligases, including the C-terminus of 70kDa heat-shock protein (Hsp70)-interacting protein (CHIP), parkin, and E4B, promote ataxin-3 ubiquitination."

sparser
"According to further studies, the C terminus of Hsc70-interacting protein (CHIP), an E3 ubiquitin ligase that ubiquitinates ataxin-3 in vitro, is dispensable for its ubiquitination in vivo and is not required for the neuroprotective function of this DUB in Drosophila."

reach
"According to a previous report, the E3 ubiquitin ligase CHIP may ubiquitinate ataxin-3 as well as other polyQ proteins to target them for degradation 39."

reach
"According to further studies, the C terminus of Hsc70 interacting protein (CHIP), an E3 ubiquitin ligase that ubiquitinates ataxin-3 in vitro, is dispensable for its ubiquitination in vivo and is not required for the neuroprotective function of this DUB in Drosophila."

reach
"E3 ubiquitin ligase CHIP along with Hsp70 ubiquitinates and degrades polyglutamine aggregates of mutant huntingtin and ataxin-3, as depicted in Figure XREF_FIG."
CK2 affects ATXN3
| 7 4
CK2 phosphorylates ATXN3.
| 6 3
CK2 phosphorylates ATXN3. 9 / 10
| 6 3

reach
"More recently, it was found that CK2 phosphorylates and promotes stabilization and aggregation of ataxin3 (ATXN3), the pathogenic protein of spinocerebellar ataxia type 3 (SCA3)."

reach
"The product of this gene, ataxin-3, associates to and is phosphorylated by CK2 175 (Fig. 3d ), which induces its nuclear localization and stabilization, and enhances the formation of inclusions."

reach
"Phosphorylations of ATX3 by protein casein kinase 2 (CK2) stimulate SCA3 pathogenesis by altering its stability, nuclear localization, and inclusion formation [50,51], while GSK3β-mediated phosphorylation inhibits ATX3 aggregation which has a protective role in SCA3 pathophysiology [94]."

reach
"The phosphorylation of ataxin-3 by CK2 was measured by in vitro phosphorylation assays."

sparser
"The phosphorylation of ataxin-3 by CK2 was measured by in vitro phosphorylation assays."

reach
"d Spinocerebellar ataxia type 3 (SCA3): CK2 associates to and phosphorylates ataxin-3, thus promoting its nuclear localization and stabilization, and enhancing the formation of inclusions."

sparser
"Results (1) Both wild type and expanded ataxin-3 interacted with CK2alpha and CK2beta in vitro. (2) In 293 cells, both wild type and expanded ataxin-3 interacted with CK2beta, but not CK2alpha. (3) CK2 phosphorylated wild type and expanded ataxin-3."

reach
"(3) CK2 phosphorylated wild type and expanded ataxin-3."

sparser
"Phosphorylations of ATX3 by protein casein kinase 2 (CK2) stimulate SCA3 pathogenesis by altering its stability, nuclear localization, and inclusion formation [ xref , xref ], while GSK3β-mediated phosphorylation inhibits ATX3 aggregation which has a protective role in SCA3 pathophysiology [ xref ]."
CK2 binds ATXN3.
| 1 1
| 1 1

sparser
"The interaction between ataxin-3 and CK2 was identified by glutathione S-transferase (GST) pull-down assay and co-immunoprecipition assay."

reach
"The interaction between ataxin-3 and CK2 was identified by glutathione S-transferase (GST) pull-down assay and co-immunoprecipition assay."
CAPN affects ATXN3
| 11
CAPN activates ATXN3. 10 / 12
| 11

reach
"This phenotype could be abolished by calpain inhibition, indicating a key role of calpain in ATXN3 aggregation."

reach
"Calpain cleavage sites have been identified along the ataxin-3 protein, supporting the hypothesis of calpain mediated ataxin-3 proteolysis."

reach
"Calpain inhibition reduces ataxin-3 cleavage alleviating neuropathology and motor impairments in mouse models of Machado-Joseph disease."

reach
"It was discovered that inhibition of Ca 2+ -dependent protease calpain suppressed aggregation of polyglutamine expanded Atxn3 in transfected cells [XREF_BIBR]."

reach
"It was discovered that inhibition of Ca 2+ -dependent protease calpain suppressed aggregation of polyglutamine expanded Atxn3 in transfected cells [XREF_BIBR]."

reach
"Based on observed fragment sizes and antibody binding, calpain mediated cleavage of ataxin-3 has been shown to occur most convincingly at amino acid 60, 221, and 260 [XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR]."

reach
"Calpain mediated ataxin-3 cleavage in the molecular pathogenesis of spinocerebellar ataxia type 3 (SCA3)."

reach
"Calpain inhibition is sufficient to suppress aggregation of polyglutamine expanded ataxin-3."

reach
"Calpain mediated cleavage of ataxin-3 has an important role in SCA3 pathogenesis [XREF_BIBR]."

reach
"It was discovered that inhibition of Ca 2+ -dependent protease calpain suppressed aggregation of polyglutamine expanded Atxn3 in transfected cells."
BP affects ATXN3
| 12
ATXN3 binds BP. 10 / 12
| 12

sparser
"Furthermore, Western blotting results of cerebellum from SCA3-BP animals obviously displayed levels of decreased p -AKT, p -ERK1/2, and p -mTOR and increased p -AMPK compared to SCA3-V and SCA3 groups ( xref F)."

sparser
"Cerebellum sections from SCA3-BP or WT were found to have less colocalized ATXN3 or polyQ with calbindin in Purkinje cells than the groups of SCA3 and SCA3-V mice."

sparser
"Next, three different scheduled euthanization samples of SCA3-BP (24-, 26-, and 28-weeks-old mice) were subjected to protein expression analysis and compared with samples acquired from WT, SCA3, and SCA3-V animals at 28 weeks of age ( xref C)."

sparser
"After five weeks of treatment ( xref B), SCA3 mice with n -BP exposure (SCA3-BP) presented a significantly improved performance in motor function and balance, determined by the accelerating rotarod tests."

sparser
"Much more abundant autophagic players were detected in SCA3-BP and WT groups compared to animals in SCA3 and SCA3-V groups (28 weeks old)."

sparser
"Thus, the number of ATXN3 /polyQ aggregates within Purkinje cells was analyzed and revealed that the cells containing aggregates more than four, either ATXN3 or polyQ, in WT and SCA3-BP groups were less than vehicle or non-treated SCA3 mice."

sparser
"Analyses of the traveled distance (middle panel) and the speed of rotarod (bottom panel) of SCA3 and SCA3-V mice falling off also presented an impressive improvement of the motor function in the SCA3-BP group."

sparser
"Results exhibited lower glucose metabolism, calculated as the standardized uptake value (SUV) ( xref A: yellow circle as ROI and quantified in xref B), in the selected regions of SCA3 than WT and SCA3-BP groups (35-week-old mice)."

sparser
"There were more observed Purkinje cells with non-fragmented neurites in WT and SCA3-BP animals (arrows indicated) compared to SCA3-V or SCA3 groups."

sparser
"However, observation of the same number of Purkinje cells in WT as compared to SCA3-BP (28-week-old) raised a question that Purkinje cells were maintained and increased."
ATXN3 affects sch1
| 12
| 12

sparser
"Mutations in FGFR1 cause autosomal dominant forms of KS [ xref ]."

sparser
"KAL2, identified as the fibroblast growth factor receptor 1 (FGFR1) gene, has now been recognised to be the underlying genetic defect for an autosomal dominant form of KS."

sparser
"Rare variants in FGFR1 were first thought to cause autosomal dominant form of KS ( xref )."

sparser
"In 2003, Fibroblast growth factor receptor 1 ( FGFR1) was identified as the first gene causing the autosomal dominant form of KS by mapping overlapping deletions on chromosome 8p11-p12 in two KS patients with contiguous gene syndromes ( xref )."

sparser
"Loss-of-function mutations in FGFR1 have also been associated with an autosomal dominant form of KS (KAL2); however this form of the disease is incompletely penetrant, indicating a more complex and probably oligogenic basis [ xref ; xref ]."

sparser
"Among them, FGFR1 is undoubtedly the most studied because mutations in FGFR1 gene ( KAL2 ) are responsible for the autosomal dominant form of KS [ xref ]."

sparser
"Various loss-of-function mutations in KAL1 , encoding the extracellular matrix glycoprotein anosmin-1, and in FGFR1 or FGF8 , encoding fibroblast growth factor receptor-1 and fibroblast growth factor-8, underlie the X-chromosome linked form and an autosomal dominant form of KS, respectively."

sparser
"Both FGFR1 and FGF8 , mapping on 8p12 and 10q24, respectively, can cause an autosomal dominant form of KS."

sparser
"KAL-2 is the gene responsible for an autosomal dominant form of KS and encodes the fibroblast growth factor type one receptor (FGFR1) [ xref ]."

sparser
"After the discovery that FGFR1 is responsible for an autosomal dominant form of KS, it was hypothesized that anosmin-1 could interact with FGFR to promote the binding of the FGF ligand [ xref ]."
ATXN3 affects HDAC6
1 | 5 4
1 | 5 4

reach
"Interestingly, ataxin 3 also binds HDAC6."

sparser
"Interestingly, ataxin 3 also binds HDAC6 ( xref )."

sparser
"Formation of aggresomes requires the activity of the deubiquitinating enzyme ataxin-3 which can bind to HDAC6 and trims both K48- and K63-linked ubiquitin chains [ xref , xref ]."

No evidence text available

sparser
"Collectively, our results suggest a novel mechanism of ataxin-3-mediated HDAC6-dependent aggresome formation, where the aggregate-specific deubiquitinase, ataxin-3, cleaves (poly)ubiquitin moieties in polyubiquitinated proteins to expose their C-terminal diglycine motifs, providing a handle for HDAC6 binding and subsequent transport to the aggresome."

reach
"Formation of aggresomes requires the activity of the deubiquitinating enzyme ataxin-3 which can bind to HDAC6 and trims both K48- and K63 linked ubiquitin chains [XREF_BIBR, XREF_BIBR]."

reach
"These results indicated the functional relationship of VCP with both ERAD (Hsp40 and Hsp70 complex) and aggresome formation (Ataxin3 and HDAC6 complex) pathways [XREF_BIBR]."

sparser
"Indeed, in ataxin 3-deficient cells, HDAC6 no longer associates with ubiquitinated protein aggregates ( xref )."

reach
"These results indicate the functional relationship of VCP with protein folding (Hsp40-Hsp70 and Hsp90 complex), ERAD (gp78 complex) and aggresome formation (Ataxin3 and HDAC6 complex, our unpublished data) pathways [XREF_BIBR]."

reach
"In addition, ataxin-3 interacts with dynein and the cytosolic histone deacetylase 6 during transport of misfolded proteins in cultured cells, thus linking ataxin-3 to cytoskeletal transport processes."
ATXN3 affects Death
| 12
ATXN3 activates Death. 8 / 8
| 8

reach
"Spinocerebellar ataxia type 3 (SCA3) is the most frequent inherited cerebellar ataxia in Europe, the US and Japan, leading to disability and death through motor complications."

reach
"These data supported an idea that, due to enhanced interaction to p53 and up-regulation of p53, polyQ expanded ATX-3 led to an increased p53 dependent neuronal cell death (including both early apoptotic and late apoptotic and necrotic manner)."

reach
"The above results indicate that the expanded polyQ tract in ataxin3 could change the DNA methylation status of a variety of genes involved in various biological processes and, thus, cause neuronal death in the cerebellum and spinal cord due to alterations in the expression of these genes."

reach
"MJD leads to premature death and there is no therapy available."
| PMC

reach
"Taken together, this study provides evidence showing that the truncated ATXN3 accelerates the formation of aggregates, disrupts the dynamics of mitochondria, and leads to neuronal death in vitro and in vivo."

reach
"It is caused by a CAG over repetition in ATXN3 gene, which translates into a mutated ataxin- 3 protein that accumulates in neurons, causing neuronal dysfunction and death."
| PMC

reach
"In addition, they found that expanded ataxin3 could activate the mitochondrial apoptotic pathway and induce neuronal death by regulating gene expression [XREF_BIBR]."

reach
"Spinocerebellar ataxia type 3 (SCA3) is caused by the expansion of a polyglutamine (polyQ) repeat in the protein ataxin-3 which is involved in susceptibility to mild oxidative stress induced neuronal death."
Mutated ATXN3 activates Death. 4 / 4
| 4

reach
"Since the mechanism by which mutant ataxin-3 eventually leads to neuronal death is poorly understood, additional investigations to clarify the biological alterations related to Machado-Joseph disease are necessary."

reach
"Moreover, amelioration of ATXN3-Q84-mediated phosphorylation of p53, c-Abl and PKCdelta by pharmacological inhibition of ATM suggests that mutant ATXN3 activates the pro death signaling cascades via activating ATM in SCA3."

reach
"These data substantiate our previous interpretation that mutant ATXN3 activates the p53 dependent pro death pathway by activating ATM, and that chronic activation of the ATM --> p53 pathway plays a pivotal role in mediating neuronal death in SCA3."

reach
"The susceptibility to death induced by the expression of mutant ataxin-3 varies among individual cell types, even among neurons [14]."
| 10
| 10

reach
"XREF_FIG), suggesting that mutant ATXN3 strongly activates the DNA damage response pathway and the polyQ sequence length is important for ATM pathway activation."

reach
", suggesting that the mutant ATXN3 induced DNA damage response ATM pathway activation is oxidation independent."

reach
"Either overexpression of PNKP or pharmacological inhibition of ATM in mutant ATXN3 expressing cells blocked aberrant activation of the pro death pathways and reduced cell death, suggesting that mutant ATXN3 mediated chronic activation of the DNA damage response ATM signaling pathway plays a pivotal role in neuronal dysfunction and neurodegeneration in SCA3."

reach
"XREF_FIG), suggesting that mutant ATXN3 strongly activates the DNA damage response pathway in vivo."

reach
"However, pre-treating cells with NAC did not block mutant ATXN3 mediated activation of the DNA damage response pathway (XREF_SUPPLEMENTARY Figs.)"

reach
"The amelioration of apoptosis by pharmacological inhibition of ATM and p53, suggest that mutant ATXN3 mediated aberrant activation of the DNA damage response pathway facilitates the apoptotic demise of neuronal cells in SCA3."

reach
"Likewise, expression of the mutant ATXN3 carrying 72 and 80 poly-glutamines (ATXN3-Q72 and ATXN3-Q80) in SH-SY5Y cells also strongly activated the DNA damage response ATM pathway (XREF_SUPPLEMENTARY)."

reach
"Mutant ATXN3 activates the DNA damage response pathway in SCA3."

reach
"Specific modulation of mutant ATXN3 mediated atypical activation of the DNA damage response p53 and PKCdelta pathways, or enhancing the efficacy of in vivo DNA damage repair may be effective strategies to combat the pathways leading to systemic neurodegeneration in SCA3."

reach
"Consistent with our hypothesis, ATXN3-Q84 expression failed to stimulate phosphorylation of Chk2 and p53 in the presence of the ATM inhibitor Ku55933 (XREF_SUPPLEMENTARY), substantiating our interpretation that mutant ATXN3 stimulates the DNA damage response p53 pathway via activating ATM."

reach
"Therefore, we conclude from these experiments that ATX3 deficiency impairs DNA damage response through regulating Chk1 specifically."

reach
"ATX3 deficiency impairs Chk1 mediated G2/M DNA damage checkpoint."
ATXN3 affects BP
| 12
ATXN3 binds BP. 10 / 12
| 12

sparser
"Furthermore, Western blotting results of cerebellum from SCA3-BP animals obviously displayed levels of decreased p -AKT, p -ERK1/2, and p -mTOR and increased p -AMPK compared to SCA3-V and SCA3 groups ( xref F)."

sparser
"Cerebellum sections from SCA3-BP or WT were found to have less colocalized ATXN3 or polyQ with calbindin in Purkinje cells than the groups of SCA3 and SCA3-V mice."

sparser
"Next, three different scheduled euthanization samples of SCA3-BP (24-, 26-, and 28-weeks-old mice) were subjected to protein expression analysis and compared with samples acquired from WT, SCA3, and SCA3-V animals at 28 weeks of age ( xref C)."

sparser
"After five weeks of treatment ( xref B), SCA3 mice with n -BP exposure (SCA3-BP) presented a significantly improved performance in motor function and balance, determined by the accelerating rotarod tests."

sparser
"Much more abundant autophagic players were detected in SCA3-BP and WT groups compared to animals in SCA3 and SCA3-V groups (28 weeks old)."

sparser
"Thus, the number of ATXN3 /polyQ aggregates within Purkinje cells was analyzed and revealed that the cells containing aggregates more than four, either ATXN3 or polyQ, in WT and SCA3-BP groups were less than vehicle or non-treated SCA3 mice."

sparser
"Analyses of the traveled distance (middle panel) and the speed of rotarod (bottom panel) of SCA3 and SCA3-V mice falling off also presented an impressive improvement of the motor function in the SCA3-BP group."

sparser
"Results exhibited lower glucose metabolism, calculated as the standardized uptake value (SUV) ( xref A: yellow circle as ROI and quantified in xref B), in the selected regions of SCA3 than WT and SCA3-BP groups (35-week-old mice)."

sparser
"There were more observed Purkinje cells with non-fragmented neurites in WT and SCA3-BP animals (arrows indicated) compared to SCA3-V or SCA3 groups."

sparser
"However, observation of the same number of Purkinje cells in WT as compared to SCA3-BP (28-week-old) raised a question that Purkinje cells were maintained and increased."
ATXN3 affects ATM
| 11 1
Mutated ATXN3 activates ATM. 9 / 9
| 9

reach
"Knockdown of normal ATXN3 or PNKP, or expression of mutant ATXN3 increased the accumulation of DNA damage and persistent activation of the ATM and p53 dependent DNA repair pathway, suggesting ATXN3 may be a key regulator of PNKP dependent DNA repair."

reach
"Our data suggest that in addition to activating the DNA damage induced p53 pathway as described earlier in SCA3 [XREF_BIBR, XREF_BIBR], mutant ATXN3 also activates the ATM dependent cAbl --> PKCdelta pro apoptotic pathway in parallel to cause neuronal dysfunction and eventually facilitating systemic neuronal degeneration in SCA3 brain (XREF_FIG)."

reach
"Our accompanying manuscript clearly shows that either PNKP depletion or the expression of mutant ATXN3 leads to ATM mediated activation of two parallel proapoptotic pathways; one is p53- and the other c-Abl --> PKCdelta mediated apoptotic cell death, a hallmark of neuropathogenesis (Gao et al.."

reach
"To investigate whether mutant ATXN3 activates ATM signaling in SCA3, we expressed ATXN3-Q84 in differentiated SH-SY5Y cells and assessed activation of the ATM pathway."

reach
"Since oxidative stress alone can activate the ATM pathway [XREF_BIBR], we sought to determine whether mutant ATXN3 activates ATM via an oxidation dependent mechanism."

reach
"These data substantiate our previous interpretation that mutant ATXN3 activates the p53 dependent pro death pathway by activating ATM, and that chronic activation of the ATM --> p53 pathway plays a pivotal role in mediating neuronal death in SCA3."

reach
"Consistent with these reports, our data show that mutant ATXN3 mediated activation of ATM --> c-Abl pathway enhanced the phosphorylation and facilitated the nuclear translocation of PKCdelta in cells, and in SCA3 transgenic mouse brains."

reach
"Mutant ATXN3 activates the pro apoptotic p53 pathway by activating ATM in SCA3."

reach
"Likewise, expression of the mutant ATXN3 carrying 72 and 80 poly-glutamines (ATXN3-Q72 and ATXN3-Q80) in SH-SY5Y cells also strongly activated the DNA damage response ATM pathway (XREF_SUPPLEMENTARY)."
ATXN3 activates ATM. 3 / 3
| 2 1

reach
"We also found that ATXN3 and eukaryotic translation initiation factor 5A2 (EIF5A2) protein levels in ATC tissues are positively correlated, and ATXN3 promotes the proliferation and metastasis of ATC cells through EIF5A2."

sparser
"Since oxidative stress alone can activate the ATM pathway [ xref ], we sought to determine whether mutant ATXN3 activates ATM via an oxidation-dependent mechanism."

reach
"Moreover, various gain/loss functional assays were performed to indicate that ATXN3 overexpression enhanced ATC cell proliferation and metastasis."
PKD affects ATXN3
| 11
ATXN3 binds PKD. 10 / 11
| 11

sparser
"The autosomal dominant form of PKD (ADPKD) primarily affects adults and is caused by mutations in the PKD1 or PKD2 genes, which encode the proteins polycystin-1 and polycystin-2, respectively."

sparser
"The autosomal dominant form of PKD (ADPKD) is twenty times more frequent than the autosomal recessive form (ARPKD) [ xref ]."

sparser
"The most prevalent form of PKD, autosomal dominant polycystic kidney disease (ADPKD), is in most cases caused by mutations in the PKD1 gene or, less commonly, in PKD2."

sparser
"The autosomal dominant form of PKD (ADPKD) results in distinct cysts, or fluid-filled spheres lined by epithelial cells."

sparser
"Until now, these abnormalities have been demonstrated mainly in the autosomal dominant form of PKD."

sparser
"At least three different genes are thought to give rise to the autosomal dominant (ADPKD) form of PKD."

sparser
"The autosomal dominant form of PKD led to the discovery of a unique family of highly complex proteins long before they would have been selected from a gene or proteomic micro-array by some desperate graduate student or fellow."

sparser
"However, gene testing has limitations as it will only identify the autosomal dominant form of PKD and not other forms of cystic kidney disease."

sparser
"PKD1 and PKD2 are mutated in the autosomal dominant form of PKD [ xref , xref ]."

sparser
"Fedeles et al. used mouse mutants to show that the main proteins implicated in ADPKD, autosomal recessive PKD and autosomal dominant polycystic liver disease functionally interact. xref Loss of glucosidase 2 subunit β or SEC63 reduces polycystin-1 and, to a lesser extent, polycystin-2 expression, blocks polycystin-1 trafficking to primary cilia, causes renal and hepatic cysts, worsens cystic disease in heterozygous Pkd1 +/− and Pkd2 +/− mice and induces renal cystogenesis inPkhd1 del4/del4 mice."
FOXO4 affects ATXN3
3 | 3 5
3 | 3 5

reach
"The interaction of ataxin-3 and FOXO4 highlighted a possible connection to the oxidative stress response."

No evidence text available

No evidence text available

sparser
"Here, we show that ATXN3 interacts with the forkhead box O (FOXO) transcription factor FOXO4 and activates the FOXO4-dependent transcription of the manganese superoxide dismutase (SOD2) gene."

sparser
"The interaction of ataxin-3 and FOXO4 highlighted a possible connection to the oxidative stress response."

sparser
"It is notable that Atxn3 interacts with mammalian FOXO4 and activates basal and stress-induced expression of SOD2 (Araujo et al. xref ); this is opposite in C. elegans where Atxn3 KO exhibits enhanced transcription of sod-3 through DAF-16 (Rodrigues et al. xref )."

reach
"It is notable that Atxn3 interacts with mammalian FOXO4 and activates basal and stress induced expression of SOD2; this is opposite in C. elegans where Atxn3 KO exhibits enhanced transcription of sod-3 through DAF-16."

sparser
"Under physiological conditions, ataxin 3 binding to FOXO4 is a necessary pre-requisite for SOD2 transcription."

reach
"In human cell lines, Ataxin-3 and ATXN3 has been shown to interact with FoxO4 and to increase FoxO dependent transcription of the gene coding for SOD-2."

sparser
"A recent study showed that in response to oxidative stress Atxn3 interacted with the transcription factor, FOXO4, and enhanced activation of manganese superoxide dismutase (SOD2) (Araujo et al. xref )."
ATXN3 affects PKD
| 11
ATXN3 binds PKD. 10 / 11
| 11

sparser
"The autosomal dominant form of PKD (ADPKD) primarily affects adults and is caused by mutations in the PKD1 or PKD2 genes, which encode the proteins polycystin-1 and polycystin-2, respectively."

sparser
"The autosomal dominant form of PKD (ADPKD) is twenty times more frequent than the autosomal recessive form (ARPKD) [ xref ]."

sparser
"The most prevalent form of PKD, autosomal dominant polycystic kidney disease (ADPKD), is in most cases caused by mutations in the PKD1 gene or, less commonly, in PKD2."

sparser
"The autosomal dominant form of PKD (ADPKD) results in distinct cysts, or fluid-filled spheres lined by epithelial cells."

sparser
"Until now, these abnormalities have been demonstrated mainly in the autosomal dominant form of PKD."

sparser
"At least three different genes are thought to give rise to the autosomal dominant (ADPKD) form of PKD."

sparser
"The autosomal dominant form of PKD led to the discovery of a unique family of highly complex proteins long before they would have been selected from a gene or proteomic micro-array by some desperate graduate student or fellow."

sparser
"However, gene testing has limitations as it will only identify the autosomal dominant form of PKD and not other forms of cystic kidney disease."

sparser
"PKD1 and PKD2 are mutated in the autosomal dominant form of PKD [ xref , xref ]."

sparser
"Fedeles et al. used mouse mutants to show that the main proteins implicated in ADPKD, autosomal recessive PKD and autosomal dominant polycystic liver disease functionally interact. xref Loss of glucosidase 2 subunit β or SEC63 reduces polycystin-1 and, to a lesser extent, polycystin-2 expression, blocks polycystin-1 trafficking to primary cilia, causes renal and hepatic cysts, worsens cystic disease in heterozygous Pkd1 +/− and Pkd2 +/− mice and induces renal cystogenesis inPkhd1 del4/del4 mice."
ATXN3 affects FOXO4
3 | 3 5
3 | 3 5

reach
"The interaction of ataxin-3 and FOXO4 highlighted a possible connection to the oxidative stress response."

No evidence text available

No evidence text available

sparser
"Here, we show that ATXN3 interacts with the forkhead box O (FOXO) transcription factor FOXO4 and activates the FOXO4-dependent transcription of the manganese superoxide dismutase (SOD2) gene."

sparser
"The interaction of ataxin-3 and FOXO4 highlighted a possible connection to the oxidative stress response."

sparser
"It is notable that Atxn3 interacts with mammalian FOXO4 and activates basal and stress-induced expression of SOD2 (Araujo et al. xref ); this is opposite in C. elegans where Atxn3 KO exhibits enhanced transcription of sod-3 through DAF-16 (Rodrigues et al. xref )."

reach
"It is notable that Atxn3 interacts with mammalian FOXO4 and activates basal and stress induced expression of SOD2; this is opposite in C. elegans where Atxn3 KO exhibits enhanced transcription of sod-3 through DAF-16."

sparser
"Under physiological conditions, ataxin 3 binding to FOXO4 is a necessary pre-requisite for SOD2 transcription."

reach
"In human cell lines, Ataxin-3 and ATXN3 has been shown to interact with FoxO4 and to increase FoxO dependent transcription of the gene coding for SOD-2."

sparser
"A recent study showed that in response to oxidative stress Atxn3 interacted with the transcription factor, FOXO4, and enhanced activation of manganese superoxide dismutase (SOD2) (Araujo et al. xref )."
AMFR affects ATXN3
2 | 7
AMFR binds ATXN3.
2 | 1
2 | 1

No evidence text available

No evidence text available

reach
"Here, we show that gp78 interacts with both SOD1 and ataxin-3."
AMFR activates ATXN3.
| 2
AMFR activates ATXN3. 2 / 3
| 2

reach
"ER associated E3 ubiquitin ligase Autocrine Motility Factor Receptor (AMFR) or Gp78 induces the degradation of Superoxide Dismutase 1 (SOD1) and ataxin-3 and provides cytoprotection against mutant SOD1 induced toxicity XREF_BIBR."

reach
"The involvement of Gp78 in neuroprotection came into existence with studies based on disease associated aggregatory proteins superoxide dismutase-1 (SOD1) and ataxin-3, which are targeted by Gp78 for ubiquitination and proteasomal degradation."
AMFR ubiquitinates ATXN3.
| 2
AMFR leads to the ubiquitination of ATXN3. 2 / 2
| 2

reach
"Gp78, an ER associated E3, also promotes ATXN3 polyubiquitination and proteasomal degradation."

reach
"The overexpression of Gp78 promotes SOD1 and ataxin-3 ubiquitination in cultured cells, whereas eliminating gp78 stabilizes them [57]."
AMFR inhibits ATXN3.
| 2
AMFR inhibits ATXN3. 2 / 2
| 2

reach
"Gp78, an ER associated E3, promotes SOD1 and ataxin-3 degradation."

reach
"Similarly, recent studies have shown that gp78 is able to enhance degradation of mutant SOD1 and ataxin-3 proteins XREF_BIBR."
SIRT1 affects ATXN3
| 10
SIRT1 inhibits ATXN3.
| 5
SIRT1 inhibits ATXN3. 3 / 3
| 3

reach
"SIRT1 overexpression decreases MJD neuroinflammation."

reach
"Furthermore, the pharmacological activation of SIRT1 with resveratrol significantly reduces motor incoordination of MJD mice."

reach
"The results show that SIRT1 overexpression significantly reduced the characteristic MJD neuropathology highlighting the role and importance of SIRT1 in the disease."
SIRT1 inhibits mutated ATXN3. 2 / 2
| 2

reach
"In addition, SIRT1 overexpression induced a decrease of mutant ataxin-3, which was prevented by chloroquine (XREF_FIG)."

reach
"Altogether, these results suggest that SIRT1 overexpression decreases accumulation and aggregation of mutant ataxin-3 in intranuclear inclusions and decreases the levels of toxic fragments, contributing to the decrease of mutant ataxin-3 toxicity."
SIRT1 activates ATXN3.
| 5
SIRT1 activates ATXN3. 3 / 3
| 3

reach
"Altogether, these results demonstrate that SIRT1 mediates the robust neuroprotective effects of CR in MJD."

reach
"We then investigated whether lentiviral overexpression of SIRT1 would be sufficient to mimic the effects of CR and mediate similar alleviation of MJD pathology."

reach
"These results suggest that in fact SIRT1 activates autophagy in MJD mice (XREF_FIG)."
SIRT1 activates mutated ATXN3. 2 / 2
| 2

reach
"Therefore, we hypothesized that SIRT1 increases mutant ataxin-3 clearance by activating autophagy."

reach
"This study indicates that in fact SIRT1 plays an important role in ataxin-3 clearance through the participation on autophagy, because the reduction of mutant ataxin-3 promoted by SIRT1 is reverted when ATG5 is genetically silenced."
PQE proteins affects ATXN3
| 10
PQE proteins inhibits ATXN3.
| 7
PQE proteins inhibits ATXN3. 7 / 7
| 7

reach
"We found that these PQE proteins promote proteasomal degradation of endogenous Atx3 and enhance its formation of aggregates."

reach
"Considering the function of HSJ1 in maintaining the balance of cellular Atx3, the PQE proteins impair the cellular proteostasis of Atx3 by sequestering HSJ1 into aggregates and causing the functional depletion or dysfunction of HSJ1 through UIM-Ub interactions."

reach
"Specially, the PQE proteins promote the proteasomal degradation of endogenous Atx3 massively, leading to a remarkable reduction of the soluble Atx3."

reach
"We have revealed for the first time that the PQE proteins disrupt the protein balance of intracellular Atx3 through sequestering the co-chaperone HSJ1 into aggregates or inclusions."

reach
"So, we conclude that the PQE proteins reduce the soluble form of intracellular Atx3 mainly through promoting proteasomal degradation."

reach
"Considering the importance of HSJ1 in maintaining the balance of cellular Atx3, we conclude that the PQE proteins impair cellular proteostasis of Atx3 through sequestering HSJ1 into aggregates and consequently causing functional loss of HSJ1."

reach
"So, we proposed that the PQE proteins reduce the soluble protein of endogenous Atx3 and thus cause decrease of the overall protein level, albeit the insoluble Atx3 aggregates increase substantially."
PQE proteins activates ATXN3.
| 3
PQE proteins activates ATXN3. 3 / 3
| 3

reach
"Together, these data reaffirm that HSJ1a suppresses the degradation of Atx3 through the UIM domain, and thus suggest that it is able to reverse the reduction of soluble Atx3 caused by the PQE proteins."

reach
"As expected, MG132 treatment aggravated the increase of the aggregated form of Atx3 caused by the PQE proteins, which may be attributed to the inefficient degradation of Atx3 upon inhibition of proteasome."

reach
"Thus, we examined the effect of HSJ1a on the degradation of endogenous Atx3 triggered by the PQE proteins."
ITPR1 affects ATXN3
| 3 6
| 1 3

sparser
"That is the isoform where the other two SD mutations were identified – the A95T mutation, a potential candidate mutation for Sézary syndrome (SS; MIM #254400), a leukemic variant cutaneous T-cell lymphoma, and the R36C mutation, identified as the cause of an autosomal dominant form of SCA29 in a mother ( de novo ) and her children (inherited) ( xref , xref )."

reach
"Liu et al. [XREF_BIBR] and Chen et al. [XREF_BIBR] found that mutant Ataxin-2 and Ataxin-3, respectively, bind the C-terminal of IP3R1 and increase the receptor 's sensitivity to activation by IP3."

sparser
"Collectively, these findings suggest that mutant ataxin-3 interacts with IP3R1, resulting in elevated calcium signaling."

sparser
"So far, there have been 16 mutations in ITPR1 associated with autosomal dominant cerebellar ataxia and 5 with dominantly inherited Gillespie syndrome ( xref and xref )."
ATXN2 binds HTT, ITPR1, and ATXN3. 3 / 3
| 3

sparser
"Importantly, mutant HTT, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of IP3R1 in rat neurons and increase its sensitivity to InsP3 [ xref , xref ]."

sparser
"My laboratory discovered that mutant Huntingtin, ataxin-2 and ataxin-3 proteins specifically binds to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP 3 R1), an intracellular Ca 2+ release channel."

sparser
"Our laboratory discovered that mutant huntingtin, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP(3)R1), an intracellular Ca(2+) release channel."
ITPR1 binds mutated ATXN3. 2 / 2
| 2

reach
"Collectively, these findings suggest that mutant ataxin-3 interacts with IP3R1, resulting in elevated calcium signaling."

reach
"In biochemical experiments, we demonstrate that mutant ATX3 (exp) specifically associated with the type 1 inositol 1,4,5-trisphosphate receptor (InsP (3) R1), an intracellular calcium (Ca (2+)) release channel."
INS affects ATXN3
| 10
| 10

sparser
"Maturity onset diabetes of the young (MODY) is an autosomal dominant form of non–insulin-dependent diabetes mellitus caused by mutations in at least 13 different genes."

sparser
"Researchers have shown that maturity onset diabetes of the young (MODY), an autosomal dominant form of non-insulin-dependent diabetes mellitus, occurs by mutation in hepatocyte nuclear factor (HNF)-1α gene. [ xref , xref ]."

sparser
"In the case of HNF1α, the latter has added importance since, in humans, mutations in the HNF1α gene are the cause of maturity onset diabetes of the young (MODY3), an autosomal dominant form of non-ins[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In addition to mutations causing MIDY, a few autosomal dominant insulin gene mutations (9 out of 39) are associated with late-onset diabetes."

sparser
"MODY, an autosomal dominant form of early-onset (before age 25) non-insulin-dependent diabetes mellitus, is a genetically heterogeneous condition."

sparser
"Recent studies have shown that mutations in this enzyme can lead to the development of a form of non-insulin-dependent diabetes mellitus that is characterized by an autosomal dominant mode of inheritance and onset during childhood."

sparser
"In humans, mutations in HNF1alpha are linked to the occurrence of maturity onset diabetes of the young type 3, an autosomal dominant form of non-insulin-dependent diabetes mellitus in which afflicted subjects generally develop hyperglycemia before 25 years of age."

sparser
"Recent studies have shown that mutations in the gene encoding this key regulatory enzyme of glycolysis are a common cause of an autosomal dominant form of non-insulin-dependent (type 2) diabetes mellitus that has an onset often during childhood."

sparser
"Last, a mutation of the glucokinase gene may lead to the development of an autosomal dominant form of non-insulin-dependent diabetes mellitus that has an onset in childhood [10-12]."

sparser
"HNF1A is a critical transcription factor whose mutations have previously been shown to be responsible for an autosomal dominant form of non-insulin-dependent diabetes mellitus, the maturity-onset diabetes of the young 3 (MODY3) xref ."

eidos
"Similarly , ATXN3 , causing spinocerebellar ataxia 3 ( SCA3 ) , interacts with RAD23A / B , which are important players in NER [ 51 ] ."

reach
"The CAG repeat expansion in the coding region of ATXN3 causes spinocerebellar ataxia type 3 (SCA3) ."

reach
"A similar study investigated the expression of ataxin3, the ortholog of ATXN3 whose polyQ pathological expansion causes Machado-Joseph disease (MJD), also known as spinocerebellar ataxia type 3 (MJD/SCA3) (Matos et al., 2019)."

reach
"Spinocerebellar ataxia type 3 (SCA3) is caused by an expanded polyglutamine stretch in ataxin-3."

reach
"Spinocerebellar Ataxia 3 (SCA3) is caused by CAG repeat expansion (polyQ) in SCA3 (a.k.a ATXN3) gene."
ATXN3 affects ITPR1
| 3 6
| 1 3

sparser
"That is the isoform where the other two SD mutations were identified – the A95T mutation, a potential candidate mutation for Sézary syndrome (SS; MIM #254400), a leukemic variant cutaneous T-cell lymphoma, and the R36C mutation, identified as the cause of an autosomal dominant form of SCA29 in a mother ( de novo ) and her children (inherited) ( xref , xref )."

reach
"Liu et al. [XREF_BIBR] and Chen et al. [XREF_BIBR] found that mutant Ataxin-2 and Ataxin-3, respectively, bind the C-terminal of IP3R1 and increase the receptor 's sensitivity to activation by IP3."

sparser
"Collectively, these findings suggest that mutant ataxin-3 interacts with IP3R1, resulting in elevated calcium signaling."

sparser
"So far, there have been 16 mutations in ITPR1 associated with autosomal dominant cerebellar ataxia and 5 with dominantly inherited Gillespie syndrome ( xref and xref )."
ATXN2 binds HTT, ITPR1, and ATXN3. 3 / 3
| 3

sparser
"Importantly, mutant HTT, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of IP3R1 in rat neurons and increase its sensitivity to InsP3 [ xref , xref ]."

sparser
"My laboratory discovered that mutant Huntingtin, ataxin-2 and ataxin-3 proteins specifically binds to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP 3 R1), an intracellular Ca 2+ release channel."

sparser
"Our laboratory discovered that mutant huntingtin, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP(3)R1), an intracellular Ca(2+) release channel."
ITPR1 binds mutated ATXN3. 2 / 2
| 2

reach
"Collectively, these findings suggest that mutant ataxin-3 interacts with IP3R1, resulting in elevated calcium signaling."

reach
"In biochemical experiments, we demonstrate that mutant ATX3 (exp) specifically associated with the type 1 inositol 1,4,5-trisphosphate receptor (InsP (3) R1), an intracellular calcium (Ca (2+)) release channel."
ATXN3 affects INS
| 10
| 10

sparser
"Maturity onset diabetes of the young (MODY) is an autosomal dominant form of non–insulin-dependent diabetes mellitus caused by mutations in at least 13 different genes."

sparser
"Researchers have shown that maturity onset diabetes of the young (MODY), an autosomal dominant form of non-insulin-dependent diabetes mellitus, occurs by mutation in hepatocyte nuclear factor (HNF)-1α gene. [ xref , xref ]."

sparser
"In the case of HNF1α, the latter has added importance since, in humans, mutations in the HNF1α gene are the cause of maturity onset diabetes of the young (MODY3), an autosomal dominant form of non-ins[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In addition to mutations causing MIDY, a few autosomal dominant insulin gene mutations (9 out of 39) are associated with late-onset diabetes."

sparser
"MODY, an autosomal dominant form of early-onset (before age 25) non-insulin-dependent diabetes mellitus, is a genetically heterogeneous condition."

sparser
"Recent studies have shown that mutations in this enzyme can lead to the development of a form of non-insulin-dependent diabetes mellitus that is characterized by an autosomal dominant mode of inheritance and onset during childhood."

sparser
"In humans, mutations in HNF1alpha are linked to the occurrence of maturity onset diabetes of the young type 3, an autosomal dominant form of non-insulin-dependent diabetes mellitus in which afflicted subjects generally develop hyperglycemia before 25 years of age."

sparser
"Recent studies have shown that mutations in the gene encoding this key regulatory enzyme of glycolysis are a common cause of an autosomal dominant form of non-insulin-dependent (type 2) diabetes mellitus that has an onset often during childhood."

sparser
"Last, a mutation of the glucokinase gene may lead to the development of an autosomal dominant form of non-insulin-dependent diabetes mellitus that has an onset in childhood [10-12]."

sparser
"HNF1A is a critical transcription factor whose mutations have previously been shown to be responsible for an autosomal dominant form of non-insulin-dependent diabetes mellitus, the maturity-onset diabetes of the young 3 (MODY3) xref ."
ATXN3 affects GEMIN4
| 10
ATXN3 inhibits GEMIN4.
| 7
ATXN3 inhibits GEMIN4. 7 / 7
| 7

reach
"It implies that polyQ expanded Atx3 impairs the function of P97 complex that is capable of down-regulating the neddylation level in cells."

reach
"However, in the pellet fractions, Atx3 100Q sequestered more P97 molecules into aggregates than Atx3 22Q, due to an increasing amount of Atx3 100Q in the pellet (XREF_FIG)."

reach
"However, aggregation of Atx3 100Q could sequester more endogenous P97 than that of Atx3 22Q (XREF_FIG)."

reach
"We have found that aggregation of polyQ expanded Atx3 can sequester P97 and Ub conjugates into the protein aggregates or inclusions through specific interactions both in vitro and in cells."

reach
"These results demonstrate that polyQ expanded Atx3 sequesters P97 molecules into insoluble aggregates or inclusions, which is dependent on the specific interaction via the VBM motif of Atx3."

reach
"PolyQ expanded Atx3 sequesters P97 to aggregates through specific interaction."

reach
"Because polyQ expanded Atx3 can sequester endogenous P97 into insoluble aggregates, we resorted to the previously established experiment to explore whether aggregation of Atx3 interferes with the function of P97."
ATXN3 binds GEMIN4.
| 3
| 3

reach
"Interestingly, mutation in the VBM motif of Atx3 100Q (Atx3 100Q / VBM *, 282 RKRR to HNHH), which disrupts the specific interaction between Atx3 and P97, significantly abolished the sequestration of P97 into aggregates; even the amount of P97 sequestered by the mutant was less than that by Atx3 22Q under the condition of similar amounts of the Atx3 aggregates (XREF_FIG)."

reach
"Because Atx3 binds P97 through a VBM motif XREF_BIBR, we applied isoform I of Atx3 (Atx3-I) as a molecular model to explore the relationship between sequestration and specific interaction (XREF_FIG)."

reach
"Moreover, Atx3 interacts with P97 and VCP through a VCP binding motif (VBM) XREF_BIBR XREF_BIBR."
6 | 3
Valproic acid decreases the amount of ATXN3.
6 |
Valproic acid decreases the amount of ATXN3. 6 / 6
6 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
Valproic acid inhibits ATXN3.
| 3

reach
"Another HDAC inhibitor, valproic acid (VPA), produces similar results in Drosophila and SCA3 cell models."

reach
"VPA suppresses apoptosis in SCA3 cell culture model."

reach
"Another HDAC inhibitor, valproic acid (VPA), produces similar results in Drosophila and SCA3 cell models."
Cmt affects ATXN3
| 9
| 9

sparser
"This mutation was considered pathogenic for an autosomal dominant form of CMT 1B. The present case is only the second case of CMT syndrome in which peripheral otopathology is available."

sparser
"The autosomal dominant form of HMSN VI is caused by a mutation in the nuclear mitofusin-2 gene, xref and constitutes a subclass of CMT2A, the most common autosomal dominant form of axonal CMT."

sparser
"This new autosomal dominant CMT neuropathy is associated with an unknown gene defect."

sparser
"The families presented here displayed a severe, predominantly motor, axonal autosomal dominant form of CMT with proximal motor involvement in the lower limb associated with two previously unreported mutations in the NEFH gene, including a de novo mutation."

sparser
"As a general rule, mutations in genes encoding peripheral myelin proteins determine primary demyelinating forms of autosomal dominant CMT (AD-CMT) diseases, whereas mutations in genes encoding neuron/[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Seven of these regions have been previously associated with autosomal dominant CMT disease, but only five of these seven regions comprised known genes associated with CMT neuropathy."

sparser
"Autosomal recessive peripheral neuropathies are relatively rare but are clinically more severe than autosomal dominant forms of CMT, and understanding their molecular basis may provide a new perspective on these mechanisms."

sparser
"The autosomal dominant forms of CMT are well characterized, but the nosology of autosomal recessive CMT is still controversial."

sparser
"Conclusions: ERG2 gene has been described as a cause of various phenotypes, including a rare autosomal dominant form of CMT (CMT type 1D) representing approximately 1% of all CMT subgroups."
USP19_b affects ATXN3
| 9
USP19_b increases the amount of ATXN3.
| 5
USP19_b increases the amount of ATXN3. 5 / 5
| 5

reach
"USP19_b up-regulates the protein levels of Atx3 and Htt-N552."

reach
"It suggests that USP19_b up-regulates the Atx3 100Q level, while increase of the protein amount leads to aggregation."

reach
"Although USP19_b also up-regulates the protein levels of endogenous Atx3, and overexpressed Atx3 22Q and Htt-N552 18Q, we have not observed the aggregates or inclusions formed by overexpressed proteins with normal polyQ lengths (Atx3 22Q, Htt-N552 18Q) in the cultured cells, even in the presence of overexpressed USP19_b (XREF_FIG)."

reach
"Furthermore, USP19_b could also up-regulate the protein level of endogenous Atx3 (XREF_FIG), albeit this increasing effect was not obvious as that on the overexpressed Atx3 22Q (XREF_SUPPLEMENTARY)."

reach
"Again, USP19_b could up-regulate the protein levels of overexpressed Atx3 and Htt-N552 with normal polyQ lengths, whereas the CS-domain mutant significantly reduced this effect (XREF_SUPPLEMENTARY)."
USP19_b activates ATXN3.
| 4
USP19_b activates ATXN3. 4 / 4
| 4

reach
"Moreover, the increase of Atx3 100Q led by USP19_b was dose dependent (XREF_SUPPLEMENTARY)."

reach
"USP19_b promotes aggregation of polyQ expanded Atx3 and Htt-N552."

reach
"We have revealed that USP19_b up-regulates the protein levels of Atx3 100Q and Htt-N552 100Q and consequently promotes their aggregation."

reach
"The result showed that USP19_b significantly increased the protein level of Atx3 100Q in a manner dependent on the ubiquitin specific protease activity (XREF_FIG)."
STAT3 affects ATXN3
| 9
| 9

sparser
"Most patients have disease due to autosomal dominant STAT3 mutations, which are associated with increased susceptibility to certain fungal infections and non-immune abnormalities including pneumatoceles [ xref , xref ]."

sparser
"Like the autosomal dominant form of HIES caused by STAT3 mutations, which is also known as Job’s syndrome, DOCK8 deficiency exhibits eczema, recurrent pneumonias, elevated serum IgE, and sometimes boils and mucocutaneous candidiasis."

sparser
"Two disease entities have been identified: an autosomal dominant form caused by a STAT3 gene mutation (OMIM#102582) [ xref ] and an autosomal recessive form primarily caused by a loss-of-function mutation in the dedicator of cytokinesis 8 (DOCK8) gene (OMIM#611432) [ xref , xref ]."

sparser
"First reported in 2009, DOCK8 deficiency was identified as the underlying abnormality in the majority of patients with autosomal recessive hyper IgE syndrome (AR-HIES) [8,9] ; the autosomal dominant [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Interestingly, mutations in STAT3 are also associated with the autosomal dominant form of HIES (hyper IgE syndrome) [ xref ], a condition in which patients have extremely elevated serum IgE levels, recurrent eczema, and an increased risk for skin and lung infections."

sparser
"Indeed, an autosomal dominant form of hyper‐IgE syndrome that is caused by STAT3 mutations is characterized by susceptibility to staphylococcal abscesses and CMC xref ."

sparser
"First reported in 2009, DOCK8 deficiency was identified as the underlying abnormality in the majority of patients with autosomal recessive Hyper IgE Syndrome (AR-HIES) [ xref , xref ]; the autosomal dominant form of this syndrome (AD-HIES) caused by signal transducer and activator of transcription 3 ( STAT3 ) mutations [ xref , xref ]."

sparser
"The impaired Th17 differentiation is notable because it replicates a finding in the autosomal dominant form of HIES (AD-HIES, also known as Job’s syndrome) associated with mutations in STAT3 [ xref ]."

sparser
"The association of heterozygous signal transducer and activator of transcription 3 (STAT3) mutations with autosomal dominant (AD)-HIES allows the differentiation of AD-HIES from disorders associated with eczema and increased serum IgE levels, such as other primary immunodeficiencies and atopic dermatitis."
RHEB affects ATXN3
| 9
| 5

sparser
"Furthermore, the ATXN3 C14A mutant co-IPed more Ub-Rheb than wild-type ATXN3 ( xref ), suggesting that the binding of inactive ATXN3 to the Ub-Rheb may prevent its deubiquitination in the cells or during the purification of the ATXN3-Rheb complex."

sparser
"ATXN3, a deubiquitinase, interacts with Rheb and decreases levels of Ub-Rheb."

sparser
"As a consequence, the interaction between Rheb and ATXN3 was reduced by amino acid stimulation ( xref and xref )."

sparser
"In fact, while the interaction between Rheb and ATXN3 was increased in cells expressing the inactive Rag heterodimer, it was diminished by the active Rag heterodimer ( xref )."

sparser
"n -BP eliminated polyQ expanded ATXN3 and did not inhibit normal ATXN3 expression, and this effect on binding of wild-type ATXN3 and Rheb to remove ubiquitin of Rheb might further affect mTORC1 activity."
| 4

sparser
"Furthermore, the ATXN3 C14A mutant co-IPed more Ub-Rheb than wild-type ATXN3 ( xref ), suggesting that the binding of inactive ATXN3 to the Ub-Rheb may prevent its deubiquitination in the cells or during the purification of the ATXN3-Rheb complex."

sparser
"As expected, the four lysine residues of Rheb, which are responsible for the polyubiquitination of Rheb, are important for the binding of Ub-Rheb with ATXN3 ( xref )."

sparser
"In fact, higher levels of Ub-Rheb were co-IPed with ATXN3 when N-Ethylmaleimide, a deubiquitinase inhibitor, was included in the lysis and purification buffers ( xref ), supporting the idea that the Ub-Rheb that binds to ATXN3 was subjected to deubiquitination during the isolation processes."

sparser
"These observations support the idea that ATXN3 translocates to the lysosome and interacts with Ub-Rheb for its deubiquitination in a manner dependent on the inactive Rag heterodimer in response to amino acid starvation."
MID1 affects ATXN3
| 3 6
| 3 1

reach
"Furthermore, we show that this binding of MID1 to ATXN2, ATXN3, and ATXN7 mRNA, similar to what we have shown previously for HTT, induces translation in a CAG repeat length dependent manner in vitro and in cell lines."

reach
"We show that ATXN2, ATXN3, and ATXN7 bind to MID1 in a CAG repeat length dependent manner."

reach
"Furthermore, we show that functionally, in line with what we have previously observed for HTT, the binding of MID1 to ATXN2, ATXN3, and ATXN7 mRNA induces protein synthesis in a repeat length dependent manner."

sparser
"Similarly, to test if MID1 binds to endogenously transcribed full-length mutant ATXN3 mRNA, we expressed FLAG-MID1 in primary neurons of a transgenic mouse line that expresses full-length mutant human ATXN3 ."
| 3

sparser
"Furthermore, we show that this binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA, similar to what we have shown previously for HTT (Krauss et al., xref ), induces translation in a CAG repeat length-dependent manner in vitro and in cell lines."

sparser
"To test if the flanking regions influence the binding of MID1 to CAG repeat mRNAs, we tested the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNAs."

sparser
"Furthermore, we show that functionally, in line with what we have previously observed for HTT, the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA induces protein synthesis in a repeat length-dependent manner."
| 2

sparser
"In this study we show that MID1 can also bind to the CAG repeat region of ATXN2, ATXN3 , and ATXN7 in vitro , suggesting that binding of MID1 to CAG repeats is not dependent on the flanking regions."

sparser
"We show that ATXN2, ATXN3 , and ATXN7 bind to MID1 in a CAG repeat length-dependent manner."
GABARAP affects ATXN3
| 2 7
| 2 4

sparser
"Similar to recombinant ATXN3, we found that ectopically expressed ATXN3 with N‐terminal histidine (10xHis) and C‐terminal hemagglutinin (HA) epitope tags ( 10xHis ATXN3 HA ) as well as endogenous ATXN3 binds GABARAP."

reach
"The Machado-Joseph disease deubiquitylase ataxin-3 interacts with LC3C and GABARAP and promotes autophagy."

sparser
"These results suggest that ATXN3 interacts with GABARAP, and likely LC3C, through multiple binding sites in its Josephin domain."

reach
"Moreover, Ataxin-3 interacts with the autophagosomal membrane proteins LC3C and GABARAP and transiently localized to early autophagosomes."

sparser
"To this end, we incubated recombinant ATXN3 with LC3A and GABARAP agarose beads, which revealed a robust interaction between ATXN3 and GABARAP, while binding of ATXN3 to LC3A was not observed (Figure xref a)."

sparser
"The screening allowed to identify six UIM-containing interactors, of which epsins (i.e., EPN1, EPN2, and EPN3) and rabenosyn interact with both LC3A and GABARAP, whereas Ataxin-3 and Ataxin-3L interact specifically with GABARAP."
| 3

sparser
"Ataxin-3 preferentially binds GABARAP and LC3C with either of its two recently identified LIR motifs."

sparser
"Moreover, Ataxin-3 interacts with the autophagosomal membrane proteins LC3C and GABARAP and transiently localized to early autophagosomes."

sparser
"ATXN3 interacts directly with GABARAP and LC3C."
CREBBP affects ATXN3
3 | 4 2
3 | 4 2

reach
"Ataxin-3 interacts with the major histone acetyltransferases cAMP-response-element binding protein (CREB)-binding protein (CBP), p300, and p300 and CREB binding protein associated factor (KAT2B and PCAF, Figures XREF_FIG and XREF_FIG), and is proposed to inhibit transcription in specific promoters (e.g., MMP-2 promoter) either by blocking access to histone acetylation sites or through recruitment of histone deacetylase 3 (HDAC3) and nuclear receptor co-repressor (NCOR1; Figures XREF_FIG and XREF_FIG)."

reach
"ATXN3 binds to CREBBP and inhibits CREBBP dependent transcriptional coactivation (Li et al., 2002)."

reach
"showed that Atxn3 interacted with three major histone acetyltransferases, CBP, p300, and p300 and CBP associated factor and inhibited their acetyltransferase activity resulting in repression of model gene promoters."

sparser
"ATXN3 binds to CREBBP and inhibits CREBBP dependent transcriptional coactivation ()."

reach
"In fact, ATXN3 interacts with and inhibits CREB binding protein, 39 a histone acetyl transferase implicated in PTEN promoter activation."

No evidence text available

No evidence text available

No evidence text available

sparser
"Moreover, normal ataxin-3 represses cAMP response element-binding protein-mediated transcription, indicating a functional consequence of ataxin-3 interactions with CBP."
COL9A2 affects ATXN3
| 9
| 9

sparser
"Exome sequencing was performed among three direct relatives (proband III:3, his wife III:4 and one of his daughters IV:4, xref ) within the reported Caucasian family with the autosomal dominant form of MED ( xref )."

sparser
"Val194Asp) has previously been shown to cause an autosomal dominant form of MED in which patients are normal at birth, but develop mild short-limbed dwarfism during childhood ( xref , xref )."

sparser
"Doubled-layered patella, a type of bipartite patella with a coronal septum that divides the patella into anterior and posterior segments, is recognized as a specific radiological feature of the recessive form of MED [Scheffield EG; 1998]; however, it was also seen in a patient with an autosomal dominant form caused by COL9A2 mutation [ xref ]."

sparser
"Autosomal dominant forms of MED are caused by mutations in the genes encoding matrilin-3 (MATN3), collagen IX chains (COL9A1, COL9A2 and COL9A3) and cartilage oligomeric matrix protein (COMP) [ xref ]."

sparser
"We report a three-generation family with an autosomal dominant form of MED, characterised by normal stature, joint pain in childhood and early-onset osteoarthrosis, affecting mainly the hips and knees."

sparser
"Missense mutations in the gene encoding matrilin-3 cause an autosomal dominant form of MED and in total eleven different mutations have now been identified in 26 unrelated families with various forms of MED (see xref )."

sparser
"For the autosomal dominant forms of MED, the mutations exert their phenotypic effect through a dominant negative mechanism, leading to reduced secretion of structurally abnormal proteins into the extracellular matrix as well as intracellular retention of the abnormal proteins within the rough endoplasmic reticulum (RER) [ xref ; xref ; xref ; xref ; xref ]."

sparser
"Autosomal dominant forms of MED can result from mutations in genes encoding type IX collagen, oligomeric cartilage protein (COMP), and matrilin-3 [ xref ]."

sparser
"Our goal was to identify a mutation causing an autosomal dominant form of MED in a large multigenerational family."
CHRN affects ATXN3
| 9
| 9

sparser
"NAChR mutations found in familial epilepsy are not always associated with an autosomal dominant mode of inheritance. alpha4-T265I is the first nAChR allele showing a markedly reduced penetrance consistent with a major gene effect."

sparser
"Missense mutations of the nAChR α4 subunit associated with autosomal dominant nocturnal frontal lobe epilepsy affected highly conserved residues in M2, specifically those residues predicted to be part of the M2-axis that rotates when the agonist binds and opens the pore."

sparser
"Even if nicotinic acetylcholine receptor genes are traditionally associated with autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE), this single-family description can open new possibilities in the genetic diagnosis, molecular characterization, and management of CHRNA2-related epilepsy."

sparser
"Examples of hyperactive disorders include gain-of-function mutations in P/Q-type calcium channels, linked to familial hemiplegic migraine type 1 (Ophoff et al., xref ; Tottene et al., xref ), or mutations in neuronal nAChRs associated to autosomal dominant nocturnal frontal lobe epilepsy (Steinlein et al., xref ; Klaassen et al., xref )."

sparser
"Steinlein O, Mulley J, Propping P, Wallace R, Phillips H, Sutherland G, Scheffer I, Berkovic S: A missense mutation in the neuronal nicotinic acetylcholine receptor a4 subunit is associated with [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Amino acid mutations in the human α4β2 nAChR associated with autosomal dominant nocturnal frontal lobe epilepsy, which when are engineered in the corresponding α4β2 nAChR in mice, lead to disturbances of inhibitory synchronization of cortical networks by GABAergic interneurons ( xref )."

sparser
"Mutations of the nicotinic acetylcholine receptor subunits are associated with autosomal dominant nocturnal frontal lobe epilepsy (De Fusco et al., xref )."

sparser
"Autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE) is associated with genetically heterogeneous mutations in the neuronal nicotinic acetylcholine receptor in several international kindreds. xref Video-EEG polysomnography is necessary for definitive diagnosis, confirming abrupt awakening, and stereotypical motor behavior with vocalization and violent or dystonic-dyskinetic movements."

sparser
"Recently, two mouse strains carrying mutant alleles of the alpha4 subunit of the nicotinic acetylcholine receptor that are associated with autosomal dominant nocturnal frontal lobe epilepsy have been found to show spontaneous seizures."
BCL2L1 affects ATXN3
2 | 1 2 3
2 | 1 2 3

No evidence text available

sparser
"Ataxin-3 directly interacts with Bcl-XL."

trips
"Ataxin-3 directly interacts with Bcl-XL."

sparser
"Recently, it was shown that a direct interaction between ataxin-3 and Bcl-xL exists and suggested that ataxin-3 promotes the interaction between Bcl-xL and Bax [ xref ]."

reach
"Ataxin-3 directly interacts with Bcl-XL."

reach
"Recently, it was shown that a direct interaction between ataxin-3 and Bcl-xL exists and suggested that ataxin-3 promotes the interaction between Bcl-xL and Bax [XREF_BIBR]."

No evidence text available

sparser
"ATX3 directly binds to Bcl-xL and promotes the interaction between Bcl-xL and Bax, which cooperate in modulating mitochondrial oxidative stress-induced apoptosis by preventing the activation of Bax (Cheng et al., xref ; Youle and Strasser, xref ; Zhou et al., xref )."
ATXN3 affects cmt
| 9
| 9

sparser
"This mutation was considered pathogenic for an autosomal dominant form of CMT 1B. The present case is only the second case of CMT syndrome in which peripheral otopathology is available."

sparser
"The autosomal dominant form of HMSN VI is caused by a mutation in the nuclear mitofusin-2 gene, xref and constitutes a subclass of CMT2A, the most common autosomal dominant form of axonal CMT."

sparser
"This new autosomal dominant CMT neuropathy is associated with an unknown gene defect."

sparser
"The families presented here displayed a severe, predominantly motor, axonal autosomal dominant form of CMT with proximal motor involvement in the lower limb associated with two previously unreported mutations in the NEFH gene, including a de novo mutation."

sparser
"As a general rule, mutations in genes encoding peripheral myelin proteins determine primary demyelinating forms of autosomal dominant CMT (AD-CMT) diseases, whereas mutations in genes encoding neuron/[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Seven of these regions have been previously associated with autosomal dominant CMT disease, but only five of these seven regions comprised known genes associated with CMT neuropathy."

sparser
"Autosomal recessive peripheral neuropathies are relatively rare but are clinically more severe than autosomal dominant forms of CMT, and understanding their molecular basis may provide a new perspective on these mechanisms."

sparser
"The autosomal dominant forms of CMT are well characterized, but the nosology of autosomal recessive CMT is still controversial."

sparser
"Conclusions: ERG2 gene has been described as a cause of various phenotypes, including a rare autosomal dominant form of CMT (CMT type 1D) representing approximately 1% of all CMT subgroups."
ATXN3 affects STAT3
| 9
| 9

sparser
"Most patients have disease due to autosomal dominant STAT3 mutations, which are associated with increased susceptibility to certain fungal infections and non-immune abnormalities including pneumatoceles [ xref , xref ]."

sparser
"Like the autosomal dominant form of HIES caused by STAT3 mutations, which is also known as Job’s syndrome, DOCK8 deficiency exhibits eczema, recurrent pneumonias, elevated serum IgE, and sometimes boils and mucocutaneous candidiasis."

sparser
"Two disease entities have been identified: an autosomal dominant form caused by a STAT3 gene mutation (OMIM#102582) [ xref ] and an autosomal recessive form primarily caused by a loss-of-function mutation in the dedicator of cytokinesis 8 (DOCK8) gene (OMIM#611432) [ xref , xref ]."

sparser
"First reported in 2009, DOCK8 deficiency was identified as the underlying abnormality in the majority of patients with autosomal recessive hyper IgE syndrome (AR-HIES) [8,9] ; the autosomal dominant [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Interestingly, mutations in STAT3 are also associated with the autosomal dominant form of HIES (hyper IgE syndrome) [ xref ], a condition in which patients have extremely elevated serum IgE levels, recurrent eczema, and an increased risk for skin and lung infections."

sparser
"Indeed, an autosomal dominant form of hyper‐IgE syndrome that is caused by STAT3 mutations is characterized by susceptibility to staphylococcal abscesses and CMC xref ."

sparser
"First reported in 2009, DOCK8 deficiency was identified as the underlying abnormality in the majority of patients with autosomal recessive Hyper IgE Syndrome (AR-HIES) [ xref , xref ]; the autosomal dominant form of this syndrome (AD-HIES) caused by signal transducer and activator of transcription 3 ( STAT3 ) mutations [ xref , xref ]."

sparser
"The impaired Th17 differentiation is notable because it replicates a finding in the autosomal dominant form of HIES (AD-HIES, also known as Job’s syndrome) associated with mutations in STAT3 [ xref ]."

sparser
"The association of heterozygous signal transducer and activator of transcription 3 (STAT3) mutations with autosomal dominant (AD)-HIES allows the differentiation of AD-HIES from disorders associated with eczema and increased serum IgE levels, such as other primary immunodeficiencies and atopic dermatitis."
ATXN3 affects RHEB
| 9
| 5

sparser
"Furthermore, the ATXN3 C14A mutant co-IPed more Ub-Rheb than wild-type ATXN3 ( xref ), suggesting that the binding of inactive ATXN3 to the Ub-Rheb may prevent its deubiquitination in the cells or during the purification of the ATXN3-Rheb complex."

sparser
"ATXN3, a deubiquitinase, interacts with Rheb and decreases levels of Ub-Rheb."

sparser
"As a consequence, the interaction between Rheb and ATXN3 was reduced by amino acid stimulation ( xref and xref )."

sparser
"In fact, while the interaction between Rheb and ATXN3 was increased in cells expressing the inactive Rag heterodimer, it was diminished by the active Rag heterodimer ( xref )."

sparser
"n -BP eliminated polyQ expanded ATXN3 and did not inhibit normal ATXN3 expression, and this effect on binding of wild-type ATXN3 and Rheb to remove ubiquitin of Rheb might further affect mTORC1 activity."
| 4

sparser
"Furthermore, the ATXN3 C14A mutant co-IPed more Ub-Rheb than wild-type ATXN3 ( xref ), suggesting that the binding of inactive ATXN3 to the Ub-Rheb may prevent its deubiquitination in the cells or during the purification of the ATXN3-Rheb complex."

sparser
"As expected, the four lysine residues of Rheb, which are responsible for the polyubiquitination of Rheb, are important for the binding of Ub-Rheb with ATXN3 ( xref )."

sparser
"In fact, higher levels of Ub-Rheb were co-IPed with ATXN3 when N-Ethylmaleimide, a deubiquitinase inhibitor, was included in the lysis and purification buffers ( xref ), supporting the idea that the Ub-Rheb that binds to ATXN3 was subjected to deubiquitination during the isolation processes."

sparser
"These observations support the idea that ATXN3 translocates to the lysosome and interacts with Ub-Rheb for its deubiquitination in a manner dependent on the inactive Rag heterodimer in response to amino acid starvation."
ATXN3 affects MID1
| 3 6
| 3 1

reach
"Furthermore, we show that this binding of MID1 to ATXN2, ATXN3, and ATXN7 mRNA, similar to what we have shown previously for HTT, induces translation in a CAG repeat length dependent manner in vitro and in cell lines."

reach
"We show that ATXN2, ATXN3, and ATXN7 bind to MID1 in a CAG repeat length dependent manner."

reach
"Furthermore, we show that functionally, in line with what we have previously observed for HTT, the binding of MID1 to ATXN2, ATXN3, and ATXN7 mRNA induces protein synthesis in a repeat length dependent manner."

sparser
"Similarly, to test if MID1 binds to endogenously transcribed full-length mutant ATXN3 mRNA, we expressed FLAG-MID1 in primary neurons of a transgenic mouse line that expresses full-length mutant human ATXN3 ."
| 3

sparser
"Furthermore, we show that this binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA, similar to what we have shown previously for HTT (Krauss et al., xref ), induces translation in a CAG repeat length-dependent manner in vitro and in cell lines."

sparser
"To test if the flanking regions influence the binding of MID1 to CAG repeat mRNAs, we tested the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNAs."

sparser
"Furthermore, we show that functionally, in line with what we have previously observed for HTT, the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA induces protein synthesis in a repeat length-dependent manner."
| 2

sparser
"In this study we show that MID1 can also bind to the CAG repeat region of ATXN2, ATXN3 , and ATXN7 in vitro , suggesting that binding of MID1 to CAG repeats is not dependent on the flanking regions."

sparser
"We show that ATXN2, ATXN3 , and ATXN7 bind to MID1 in a CAG repeat length-dependent manner."
ATXN3 affects GABARAP
| 2 7
| 2 4

sparser
"Similar to recombinant ATXN3, we found that ectopically expressed ATXN3 with N‐terminal histidine (10xHis) and C‐terminal hemagglutinin (HA) epitope tags ( 10xHis ATXN3 HA ) as well as endogenous ATXN3 binds GABARAP."

reach
"The Machado-Joseph disease deubiquitylase ataxin-3 interacts with LC3C and GABARAP and promotes autophagy."

sparser
"These results suggest that ATXN3 interacts with GABARAP, and likely LC3C, through multiple binding sites in its Josephin domain."

reach
"Moreover, Ataxin-3 interacts with the autophagosomal membrane proteins LC3C and GABARAP and transiently localized to early autophagosomes."

sparser
"To this end, we incubated recombinant ATXN3 with LC3A and GABARAP agarose beads, which revealed a robust interaction between ATXN3 and GABARAP, while binding of ATXN3 to LC3A was not observed (Figure xref a)."

sparser
"The screening allowed to identify six UIM-containing interactors, of which epsins (i.e., EPN1, EPN2, and EPN3) and rabenosyn interact with both LC3A and GABARAP, whereas Ataxin-3 and Ataxin-3L interact specifically with GABARAP."
| 3

sparser
"Ataxin-3 preferentially binds GABARAP and LC3C with either of its two recently identified LIR motifs."

sparser
"Moreover, Ataxin-3 interacts with the autophagosomal membrane proteins LC3C and GABARAP and transiently localized to early autophagosomes."

sparser
"ATXN3 interacts directly with GABARAP and LC3C."
ATXN3 affects DNA Damage
| 9
Mutated ATXN3 activates DNA Damage. 6 / 6
| 6

reach
"Discovery of the mechanism by which mutant ATXN3 induces DNA damage and amplifies the pro death signaling pathways provides a molecular basis for neurodegeneration due to PNKP inactivation in SCA3, and for the first time offers a possible approach to treatment."

reach
"Mutant ATXN3 induces genomic DNA damage in SCA3."

reach
"Knockdown of normal ATXN3 or PNKP, or expression of mutant ATXN3 increased the accumulation of DNA damage and persistent activation of the ATM and p53 dependent DNA repair pathway, suggesting ATXN3 may be a key regulator of PNKP dependent DNA repair."

reach
"In conclusion, our current study provides compelling evidence of how mutant ATXN3 impedes the enzymatic activity of PNKP, and induces DNA damage that manifests with activation of the pro apoptotic signaling pathways in SCA3."

reach
"Recent studies have shown elevated levels of DNA damage and strand breaks in peripheral blood lymphocytes in SCA3 patients [XREF_BIBR], indicating that mutant ATXN3 can induce DNA damage."

reach
"Since mutant ATXN3 interacts with and inactivates PNKP, we next investigated whether ectopic expression of mutant ATXN3 induces DNA damage."
| 3

reach
"Enhanced accumulation of DNA damage has been observed in SCA3 patient post-mortem tissue and transgenic animal models of SCA3."

reach
"Recently it was shown that the expression of polyQ expanded ataxin-3 can lead to genomic DNA damage in neuroblasts through inactivation of polynucleotide kinase 3 '-phosphatase (PNKP) XREF_BIBR."

reach
"Additionally, a MJD and SCA3 in vitro model demonstrated that expanded ATX3 impairs the cell 's ability to respond to stress, alters antioxidant enzyme activities, and promotes mitochondrial DNA damage, which may lead to mitochondrial dysfunction."
ATXN3 affects CREBBP
3 | 4 2
3 | 4 2

reach
"Ataxin-3 interacts with the major histone acetyltransferases cAMP-response-element binding protein (CREB)-binding protein (CBP), p300, and p300 and CREB binding protein associated factor (KAT2B and PCAF, Figures XREF_FIG and XREF_FIG), and is proposed to inhibit transcription in specific promoters (e.g., MMP-2 promoter) either by blocking access to histone acetylation sites or through recruitment of histone deacetylase 3 (HDAC3) and nuclear receptor co-repressor (NCOR1; Figures XREF_FIG and XREF_FIG)."

reach
"ATXN3 binds to CREBBP and inhibits CREBBP dependent transcriptional coactivation (Li et al., 2002)."

reach
"showed that Atxn3 interacted with three major histone acetyltransferases, CBP, p300, and p300 and CBP associated factor and inhibited their acetyltransferase activity resulting in repression of model gene promoters."

sparser
"ATXN3 binds to CREBBP and inhibits CREBBP dependent transcriptional coactivation ()."

reach
"In fact, ATXN3 interacts with and inhibits CREB binding protein, 39 a histone acetyl transferase implicated in PTEN promoter activation."

No evidence text available

No evidence text available

No evidence text available

sparser
"Moreover, normal ataxin-3 represses cAMP response element-binding protein-mediated transcription, indicating a functional consequence of ataxin-3 interactions with CBP."
ATXN3 affects COL9A2
| 9
| 9

sparser
"Exome sequencing was performed among three direct relatives (proband III:3, his wife III:4 and one of his daughters IV:4, xref ) within the reported Caucasian family with the autosomal dominant form of MED ( xref )."

sparser
"Val194Asp) has previously been shown to cause an autosomal dominant form of MED in which patients are normal at birth, but develop mild short-limbed dwarfism during childhood ( xref , xref )."

sparser
"Doubled-layered patella, a type of bipartite patella with a coronal septum that divides the patella into anterior and posterior segments, is recognized as a specific radiological feature of the recessive form of MED [Scheffield EG; 1998]; however, it was also seen in a patient with an autosomal dominant form caused by COL9A2 mutation [ xref ]."

sparser
"Autosomal dominant forms of MED are caused by mutations in the genes encoding matrilin-3 (MATN3), collagen IX chains (COL9A1, COL9A2 and COL9A3) and cartilage oligomeric matrix protein (COMP) [ xref ]."

sparser
"We report a three-generation family with an autosomal dominant form of MED, characterised by normal stature, joint pain in childhood and early-onset osteoarthrosis, affecting mainly the hips and knees."

sparser
"Missense mutations in the gene encoding matrilin-3 cause an autosomal dominant form of MED and in total eleven different mutations have now been identified in 26 unrelated families with various forms of MED (see xref )."

sparser
"For the autosomal dominant forms of MED, the mutations exert their phenotypic effect through a dominant negative mechanism, leading to reduced secretion of structurally abnormal proteins into the extracellular matrix as well as intracellular retention of the abnormal proteins within the rough endoplasmic reticulum (RER) [ xref ; xref ; xref ; xref ; xref ]."

sparser
"Autosomal dominant forms of MED can result from mutations in genes encoding type IX collagen, oligomeric cartilage protein (COMP), and matrilin-3 [ xref ]."

sparser
"Our goal was to identify a mutation causing an autosomal dominant form of MED in a large multigenerational family."
ATXN3 affects CHRN
| 9
| 9

sparser
"NAChR mutations found in familial epilepsy are not always associated with an autosomal dominant mode of inheritance. alpha4-T265I is the first nAChR allele showing a markedly reduced penetrance consistent with a major gene effect."

sparser
"Missense mutations of the nAChR α4 subunit associated with autosomal dominant nocturnal frontal lobe epilepsy affected highly conserved residues in M2, specifically those residues predicted to be part of the M2-axis that rotates when the agonist binds and opens the pore."

sparser
"Even if nicotinic acetylcholine receptor genes are traditionally associated with autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE), this single-family description can open new possibilities in the genetic diagnosis, molecular characterization, and management of CHRNA2-related epilepsy."

sparser
"Examples of hyperactive disorders include gain-of-function mutations in P/Q-type calcium channels, linked to familial hemiplegic migraine type 1 (Ophoff et al., xref ; Tottene et al., xref ), or mutations in neuronal nAChRs associated to autosomal dominant nocturnal frontal lobe epilepsy (Steinlein et al., xref ; Klaassen et al., xref )."

sparser
"Steinlein O, Mulley J, Propping P, Wallace R, Phillips H, Sutherland G, Scheffer I, Berkovic S: A missense mutation in the neuronal nicotinic acetylcholine receptor a4 subunit is associated with [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Amino acid mutations in the human α4β2 nAChR associated with autosomal dominant nocturnal frontal lobe epilepsy, which when are engineered in the corresponding α4β2 nAChR in mice, lead to disturbances of inhibitory synchronization of cortical networks by GABAergic interneurons ( xref )."

sparser
"Mutations of the nicotinic acetylcholine receptor subunits are associated with autosomal dominant nocturnal frontal lobe epilepsy (De Fusco et al., xref )."

sparser
"Autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE) is associated with genetically heterogeneous mutations in the neuronal nicotinic acetylcholine receptor in several international kindreds. xref Video-EEG polysomnography is necessary for definitive diagnosis, confirming abrupt awakening, and stereotypical motor behavior with vocalization and violent or dystonic-dyskinetic movements."

sparser
"Recently, two mouse strains carrying mutant alleles of the alpha4 subunit of the nicotinic acetylcholine receptor that are associated with autosomal dominant nocturnal frontal lobe epilepsy have been found to show spontaneous seizures."
ATXN3 affects BCL2L1
2 | 1 2 3
2 | 1 2 3

No evidence text available

sparser
"Ataxin-3 directly interacts with Bcl-XL."

trips
"Ataxin-3 directly interacts with Bcl-XL."

sparser
"Recently, it was shown that a direct interaction between ataxin-3 and Bcl-xL exists and suggested that ataxin-3 promotes the interaction between Bcl-xL and Bax [ xref ]."

reach
"Ataxin-3 directly interacts with Bcl-XL."

reach
"Recently, it was shown that a direct interaction between ataxin-3 and Bcl-xL exists and suggested that ataxin-3 promotes the interaction between Bcl-xL and Bax [XREF_BIBR]."

No evidence text available

sparser
"ATX3 directly binds to Bcl-xL and promotes the interaction between Bcl-xL and Bax, which cooperate in modulating mitochondrial oxidative stress-induced apoptosis by preventing the activation of Bax (Cheng et al., xref ; Youle and Strasser, xref ; Zhou et al., xref )."
ATXN3 affects AI
| 9
ATXN3 binds AI. 9 / 9
| 9

sparser
"Autosomal recessive forms of AI are the most prevalent in the Middle East and some parts of Asia, while the autosomal dominant forms of AI are the most prevalent in the United States and Europe [ xref ; xref ]."

sparser
"While autosomal dominant forms of AI are present in Turkey, they are not as frequent as ARAI forms."

sparser
"Recently mutations in the FAM83H gene have been identified with autosomal dominant hypoplastic forms of AI in Korea and in Turkey [ xref ; xref ; xref ]."

sparser
"In contrast to Europe and North America, where autosomal dominant forms of AI are the most common, ARAI are the most prevalent forms in Turkey, probably due to the higher rates of parental consanguinity [ xref ; xref ; xref ]."

sparser
"Human ENAM gene mutations cause autosomal dominant forms of AI ( xref ; xref )."

sparser
"33 The autosomal dominant forms of hypocalcified AI are associated with 20 defects in exon 5 of the FAM83H gene."

sparser
"The most prevalent autosomal dominant (AD) form of AI is commonly caused by the ENAM gene mutations, while the X-linked form of AI is caused by alterations in the AMELX gene xref ."

sparser
"Studies related to the autosomal dominant forms of AI, representing approximately 85% of all cases, have established linkage to 4q21 for two forms: local hypoplastic and smooth hypoplastic AI."

sparser
"We have performed a genome-wide scan in a large Brazilian family segregating an autosomal dominant form of AI and mapped a novel locus to 8q24.3."
Smo-1 affects ATXN3
| 6 2
Smo-1 inhibits ATXN3.
| 5
Smo-1 inhibits ATXN3. 5 / 5
| 5

reach
"These findings, together with the effects of the autophagy inhibitor 3-MA, led us to speculate as to whether autophagy-lysosome system plays more important role in SUMO induced ataxin-3 degradation."

reach
"On the other hand, the observation that MG132 blocked SUMO induced degradation of ataxin-3, although the effect was not strong, suggested a role of UPS for this process."

reach
"In the future studies, different substitutions should be designed and tested to confirm the requirement of SIM for SUMO induced degradation of ataxin-3."

reach
"Proteolytic pathways mediating SUMO induced ataxin-3 degradation."

reach
"Interestingly, the autophagy inhibitor 3-MA reduced SUMO induced degradation of ataxin-3 much more effectively."
Smo-1 binds ATXN3.
| 1 2
| 1 2

sparser
"Using a conventional immunoprecipitation method, we could not detect SUMO-modified form of ataxin-3 (Jung & Lee xref )."

reach
"To address whether ataxin-3 can associate with SUMO, we used recombinant SUMO1 or SUMO2 conjugated to beads to perform pull-down assays from cell lysates."

sparser
"While ATXN3 can bind SUMO and be SUMOylated ( xref ; xref ), it is not yet clear how SUMO binding or SUMOylation alters ATXN3 function or clearance."
| 4

reach
"Trehalose mediated improvements in motor behaviour were associated with a reduction of the MJD associated neuropathology, as MJD transgenic mice treated with trehalose presented preservation of cerebellar layers thickness and a decrease in the size of ataxin-3 aggregates in Purkinje cells."

reach
"Trehalose mediated improvements in motor behaviour were associated with a reduction of the MJD associated neuropathology, as MJD transgenic mice treated with trehalose presented preservation of cerebellar layers thickness and a decrease in the size of ataxin-3 aggregates in Purkinje cells."

reach
"Therefore, at 28weeks of treatment, a footprint analysis was additionally performed to investigate whether trehalose could rescue limb and gait ataxia of MJD transgenic mice."

reach
"XREF_FIG c a close to significant increase in the stride length (Control = 6.02 +/-0.12 cm, 2% trehalose = 6.51 +/-0.18 cm, p = 0.052, Student 's t test) and a significant decrease in the front base width (Control = 2.01 +/-0.08 cm, 2% trehalose = 1.77 +/-0.07 cm, p = 0.047; Student 's t test) was observed in MJD transgenic females treated with 2% trehalose, revealing that trehalose reduced the gait deficits of MJD transgenic females."
| 2

reach
"However, we observed that trehalose significantly decreased the diameter of mutant ataxin-3 aggregates in a representative lobule (lobule IX) of the cerebellum (Control = 1.91 +/-0.03 microm; 2% trehalose = 1.80 +/-0.03 microm; p = 0.0197; Fig."

reach
"Trehalose reduces cerebellar atrophy and mutant ataxin-3 aggregate size in Purkinje cells of Machado-Joseph disease transgenic mice."
Alpha,alpha-trehalose decreases the amount of ATXN3.
| 2
Alpha,alpha-trehalose decreases the amount of mutated ATXN3. 2 / 2
| 2

reach
"Trehalose activates autophagy and reduces mutant ataxin-3 protein levels in neuro-2a cells expressing mutant ataxin-3."

reach
"Altogether, these data shows that trehalose activates autophagy and reduces mutant ataxin-3 levels in this in vitro model of MJD."
RNF8 affects ATXN3
| 6 2
| 6 2

sparser
"Since we confirmed an interaction between ATX3 and RNF8 in vivo (Fig  xref ), we analysed whether ATX3 regulates RNF8 homeostasis."

reach
"Since we confirmed an interaction between ATX3 and RNF8 in vivo (Fig 3), we analysed whether ATX3 regulates RNF8 homeostasis."

sparser
"However, under our experimental conditions, we found that ATX3 forms a complex with RNF8 but not MDC1."

reach
"However, under our experimental conditions, we found that ATX3 forms a complex with RNF8 but not MDC1."

reach
"Our results reveal a mechanism to explain how the p97–ATX3RNF8 complex fine‐tunes DSB repair by preventing excessive RNF8‐dependent K63‐Ub modifications and 5′‐DNA end resection and consequently promoting the NHEJ pathway, an essential pathway for cell survival after IR and thus cancer cell resistance to radiotherapy (Jeggo et al, 2011; Jeggo & Lobrich, 2015)."

reach
"However, further studies are required to address this important question in the context of the p97–ATX3RNF8 complex and chromatin dynamics after DSB formation.While our manuscript was in preparation, Pfeiffer et al (2017) reported that ATX3 is a DUB acting at DSB sites and removes ubiquitinated chains from MDC1 to prevent a premature removal of MDC1 from the breaks."

reach
"To elucidate mechanistic insight of the p97–ATX3RNF8 complex in DSB repair, we focused on sites of DNA lesions induced by either IR or UV‐laser micro‐irradiation."

reach
"Overall, these results suggest that RNF8 forms a complex with the p97 system composed of p97, Npl4–Ufd1 and ATX3 and that p97 and ATX3 bind RNF8 independently (Fig 3H)."
IAPP affects ATXN3
| 8
| 8

sparser
"HCHWA-I ( xref , xref ), also called hereditary CysC amyloid angiopathy (HCCAA) ( xref ), is an autosomal dominant form of cerebral amyloid angiopathy (CAA)."

sparser
"The hereditary APP E693Q mutation has been described as an autosomal dominant form of cerebral amyloid angiopathy (CAA) with cerebral hemorrage xref ."

sparser
"16 Previous studies showed the formation of insoluble amyloid fibrils by non-expanded ataxin-3, 27–29 and specifically by the isolated Josephin domain."

sparser
"Autosomal dominant amyloid angiopathy is associated with bleeds.[ xref xref xref xref xref ]"

sparser
"Using human ataxin-3 containing a non-pathological number of glutamine residues (14Q), the C. elegans (1Q) orthologue we were able to (a) prove that the glutamine expansion is not essential for atax[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Temperature-dependent, irreversible formation of amyloid fibrils by a soluble human ataxin-3 carrying a moderately expanded polyglutamine stretch (Q36)."

sparser
"Familial amyloidosis, Finnish type (FAF), is an autosomal dominant form of familial amyloid polyneuropathy."

sparser
"Familial British dementia (FBD) is an autosomal dominant form of dementia neuropathologically characterized by parenchymal amyloid and preamyloid deposits, extensive cerebral amyloid angiopathy, and neurofibrillary tangles."
ATXN3 affects SUMO1
2 | 3 1
2 | 3 1

sparser
"The interaction between ATX3 and SUMO1, mediated by N-terminal Josephin domain and further stimulated by DNA damage, is indispensable for the localization of ATX3 to DSBs (Pfeiffer et al., xref )."

No evidence text available

No evidence text available

reach
"We observed that endogenous ataxin-3 interacted with SUMO1, whereas hardly any interaction with SUMO2 could be detected under our assay conditions (Fig XREF_FIG A)."

reach
"In summary, our experiments show that ataxin-3 interacts with SUMO1 and that SUMOylation is required for the recruitment of ataxin-3 to DSBs."

reach
"Additionally, our findings reveal that ataxin-3 can directly interact with SUMO1 and that this interaction stimulates the deubiquitylating activity of the enzyme."
ATXN3 affects IAPP
| 8
| 8

sparser
"HCHWA-I ( xref , xref ), also called hereditary CysC amyloid angiopathy (HCCAA) ( xref ), is an autosomal dominant form of cerebral amyloid angiopathy (CAA)."

sparser
"The hereditary APP E693Q mutation has been described as an autosomal dominant form of cerebral amyloid angiopathy (CAA) with cerebral hemorrage xref ."

sparser
"16 Previous studies showed the formation of insoluble amyloid fibrils by non-expanded ataxin-3, 27–29 and specifically by the isolated Josephin domain."

sparser
"Autosomal dominant amyloid angiopathy is associated with bleeds.[ xref xref xref xref xref ]"

sparser
"Using human ataxin-3 containing a non-pathological number of glutamine residues (14Q), the C. elegans (1Q) orthologue we were able to (a) prove that the glutamine expansion is not essential for atax[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Temperature-dependent, irreversible formation of amyloid fibrils by a soluble human ataxin-3 carrying a moderately expanded polyglutamine stretch (Q36)."

sparser
"Familial amyloidosis, Finnish type (FAF), is an autosomal dominant form of familial amyloid polyneuropathy."

sparser
"Familial British dementia (FBD) is an autosomal dominant form of dementia neuropathologically characterized by parenchymal amyloid and preamyloid deposits, extensive cerebral amyloid angiopathy, and neurofibrillary tangles."
ATXN3 affects GSK3B
4 | 4
4 | 4

No evidence text available

No evidence text available

No evidence text available

reach
"These results further support that ataxin-3 interacts with GSK 3beta."

reach
"To investigate if there is a physical interaction between ataxin-3 and GSK 3beta, we evaluated the binding of ataxin-3 with GSK 3beta using an in vitro GST pull-down assay."

No evidence text available

reach
"These data suggest that both normal and expanded ataxin-3 interact with GSK 3beta in vitro."

reach
"As ataxin-3 interacts with GSK 3beta, we further determine whether GSK 3beta phosphorylates ataxin-3."
| 4
ATXN3 inhibits Cell Survival.
| 2

reach
"ATX3 deficiency results in pronounced reduction of Chk1 abundance, compromised DNA damage response, G2/M checkpoint defect and decreased cell survival after replication stress, which can all be rescued by ectopic expression of ATX3."

reach
"Aggregation of expanded ataxin-3 in PC6-3 ataxin-3 (Q108) cells decreased cell survival only in the presence of high 3-NP concentrations, compared to HEK EGFP-ataxin-3 (Q84) cells or MJD cerebellar granule cells, which may account for a lower expression of the transgene in these cells."
ATXN3 activates Cell Survival.
| 2
| 2

reach
"Altogether, these results further support our findings that ATX3 selectively fine‐tunes DSB repair by preventing extensive RNF8/K63‐ubiquitin signalling, which balances DSB repair pathway choice.Finally, if this model is correct, then removal of RNF8 in ATX3‐depleted cells should prevent excessive 5′‐DNA end resection and restore cell survival after IR."

reach
"Based on recent studies, we suggest that wildtype ATXN3 stabilizes, or regulates, proteins at various step of the DDR process to promote efficient repair and cell survival."
UBQLN2 affects ATXN3
| 5 2
UBQLN2 binds ATXN3.
| 3 2
| 3 2

reach
"However, our assessment of coprecipitated ubiquitin in these studies did not reveal ubiquitinated HTT species and neither ruled in or out a ubiquitin dependent interaction.To examine whether ATXN3 sim[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"To examine whether ATXN3 similarly interacts with UBQLN2, we coexpressed flag-UBQLN2 or truncated UBQLN2 mutants with normal (28Q) or expanded (84Q) GFPATXN3 in HEK293 cells."

reach
"Given the difference in recruitment of UBQLN2 to disease protein inclusions in HD and SCA3, we investigated whether UBQLN2 interacts differentially with HTT and ATXN3."

sparser
"Consistent with this result, in a cell-based system mutant HTT interacts with UBQLN2 through the UBA domain while the SCA3 disease protein ATXN3, a deubiquitinating enzyme, does not interact with UBQLN2."

sparser
"To examine whether ATXN3 similarly interacts with UBQLN2, we coexpressed flag-UBQLN2 or truncated UBQLN2 mutants with normal (28Q) or expanded (84Q) GFPATXN3 in HEK293 cells."
UBQLN2 activates ATXN3.
| 2
UBQLN2 activates ATXN3. 2 / 2
| 2

reach
"Remarkably, instead of reducing the accumulation of nuclear mutant ATXN3, UBQLN2 induced an accumulation of cytoplasmic ATXN3 aggregates in neurons of SCA3 mice."

reach
"These findings suggest that ATXN3 may not be a substrate for UBQLN2 mediated clearance.In HD, SCA3, and other polyQ disorders, impairment of protein homeostasis and sequestration of clearance related [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
NRL affects ATXN3
| 7
| 7

sparser
"Missense mutations in human NRL have been associated with autosomal dominant retinitis pigmentosa."

sparser
"Missense mutations in NRL are associated with autosomal dominant retinitis pigmentosa; however, the phenotype associated with the loss of NRL function in humans has not been reported."

sparser
"Mutations in the human NRL gene are associated with autosomal dominant retinitis pigmentosa (adRP) and other retinopathies [ xref - xref ]."

sparser
"Missense mutations in the human NRL gene are associated with autosomal dominant retinitis pigmentosa, whereas the loss of its function leads to rodless retina in Nrl-knockout mice that exhibit enhanced S-cone function."

sparser
"In the human, missense mutations in NRL are associated with autosomal dominant retinitis pigmentosa [ xref , xref ]."

sparser
"Missense mutations in NRL are associated with autosomal dominant retinitis pigmentosa ( xref ; xref ; xref )."

sparser
"In humans, missense mutations in NRL are associated with autosomal dominant retinitis pigmentosa [ xref ]."
NCOR1 affects ATXN3
4 | 3
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
| 3

sparser
"Both normal (Q23) and expanded (Q70) human full-length Ataxin-3 physiologically interacted with HDAC3 and NCoR in a SCA3 rat cell line and human pons tissue."

sparser
"A direct interaction of ATXN3 with HDAC3 and NCoR has previously been established in SCA3 cell lines and human brain extracts yet with only normal (i.e. not expanded) ATXN3 being associated with increased deacetylase activity and repression of gene expression [ xref ]."

sparser
"Further, it was shown that both normal and expanded ataxin 3 physiologically interact with HDAC3 and NCoR in a SCA3 cell model and in human pons tissue; however, normal ataxin 3-containing protein complexes showed increased histone deacetylase activity, whereas polyglutamine-expanded ataxin 3-containing complexes had reduced deacetylase activity in target chromatin regions [ xref ]."

sparser
"Familial hemiplegic migraine type 2, an autosomal dominant form of migraine with aura, has been associated with four distinct mutations in the alpha2-subunit of the Na + ,K + -ATPase that is expressed primarily in astrocytes."

sparser
"In humans, mutations in the ATP1A2 gene result in familial hemiplegic migraines type II (FHM2), which are a rare autosomal dominant form of migraine with aura ( xref )."

sparser
"Familial hemiplegic migraine type 2 (FHM2) is an autosomal dominant form of migraine with aura that is caused by mutations of the α2-subunit of the Na,K-ATPase, an isoform almost exclusively expressed in astrocytes in the adult brain."

sparser
"Mutations in three genes encoding neural ion channels, CACNA1A, ATP1A2, and SCN1A, have been described in patients with familial hemiplegic migraine (FHM), a rare monogenic, autosomal dominant form of migraine with aura [ xref ]."

sparser
"FHM1 is a rare autosomal dominant form of migraine with aura, including transitory motor weakness."

sparser
"Familial hemiplegic migraine (FHM) is a rare autosomal dominant form of migraine with aura."

sparser
"As previously described for FHM3, FHM1 is a rare, autosomal dominant form of migraine with aura, characterized by recurrent attacks of disabling headache and, in some cases, progressive cerebellar atrophy."
MDC1 affects ATXN3
2 | 3 1
2 | 3 1

reach
"These revealed an interaction between endogenous MDC1 and endogenous ataxin-3, which was not enhanced by DNA damage (Fig XREF_FIG A)."

No evidence text available

reach
"These findings suggest that the interaction between ataxin-3 and MDC1 is constitutive and does not require the DNA damage induced SUMOylation of MDC1."

No evidence text available

sparser
"Furthermore, the authors failed to detect the interaction between MDC1 and ATX3 observed by Pfeiffer et al. ( xref )."

reach
"In contrast, we can detect a constitutive interaction between MDC1 and ataxin-3 that is neither enhanced by DNA damage, nor dependent on the primary SUMOylation site (K1840) in MDC1 (Luo etal, 2012)."
LRRK2 affects ATXN3
| 7
| 7

sparser
"This observation is consistent with finding of association of human LRRK2 mutations with autosomal dominant form of PD."

sparser
"Mutations in the protein kinase LRRK2 are associated with rare forms of autosomal dominant Parkinson's disease and patients carrying LRRK2 mutations present with symptoms resembling idiopathic Parkinson's disease xref , xref ."

sparser
"Mutations in LRRK2 are associated with both autosomal dominant familial and sporadic forms of PD."

sparser
"Mutations in leucine rich repeat kinase 2 (LRRK2), which are associated with autosomal dominant Parkinson’s disease, elicit progressive dendrite degeneration in neurons."

sparser
"LRRK2 mutations are also associated with autosomal dominant forms of PD ( xref )."

sparser
"Mutations in LRRK2 are associated with autosomal dominant PD, and common genetic variants are associated with an increased risk of developing sporadic PD [ xref ]."

sparser
"Autosomal dominant mutations in LRRK2 are associated with both familial and late-onset PD and patients have clinical symptoms and pathology typical of sporadic PD ( xref )."
LGI1 affects ATXN3
| 7
| 7

sparser
"Mutations in the LGI1 gene are associated with the autosomal dominant lateral temporal epilepsy (ADLTE) syndrome ( xref ), also known as autosomal dominant partial epilepsy with auditory features ( xref )."

sparser
"Mutations in the LGI1 gene are associated with a form of human autosomal dominant epilepsy ( xref ; xref )."

sparser
"LGI1 cysteine mutations have been associated with autosomal dominant lateral temporal lobe epilepsy [ xref ]."

sparser
"Prevailing evidence demonstrates that Lgi1 is tightly associated with autosomal dominant lateral temporal lobe epilepsy (ADLTE; Senechal et al., xref ; Sirerol-Piquer et al., xref ; Head et al., xref )."

sparser
"LGI1 is a secreted neuronal protein that interacts with presynaptic and postsynaptic receptors, and mutations of LGI1 have been associated with the syndrome of autosomal dominant lateral temporal lobe epilepsy [ xref , xref ]."

sparser
"LGI2 mutations have not been associated with human epilepsy, but autosomal dominant lateral temporal lobe epilepsy (ADLTE) is associated with mutations in LGI1, which interacts with the same ADAM-family receptors."
| PMC

sparser
"Interestingly, LGI1 mutations have been associated with an autosomal dominant lateral temporal lobe epilepsy manifesting often with auditory features ( xref )."
FBXL3 affects ATXN3
| 7
FBXL3 inhibits ATXN3.
| 5
FBXL3 inhibits ATXN3. 5 / 5
| 5

reach
"While the specific mechanism by which FBXL3 mediates reduction of ATXN3 abundance remains to be defined, the development of small molecules that activate this mechanism could be of therapeutic value for SCA3."

reach
"To evaluate if FBXL3 mediated reduction of ATXN3 abundance occurs via the SCF and CUL1 ubiquitination complex, we co-electroporated plasmid overexpressing FBXL3 while also decreasing CUL1 with siRNAs against CUL1 (XREF_FIG)."

reach
"If FBXL3 knockdown increases ATXN3 abundance, then its overexpression would be expected to decrease ATXN3 protein levels."

reach
"Knockdown of CUL1 abolished FBXL3 mediated reduction of normal ATXN3, but only accounted for about half of the observed FBXL3 facilitated decrease of pathogenic ATXN3 (XREF_FIG), suggesting that FBXL3 handles or recognizes normal and mutant ATXN3 differently."

reach
"Overexpression of FBXL3 suppresses ATXN3 abundance in a CUL1 dependent manner in SCA3 neuronal progenitor cells (NPCs)."
FBXL3 decreases the amount of ATXN3.
| 2
FBXL3 decreases the amount of ATXN3. 2 / 2
| 2

reach
"ATXN3 transcript levels, although variable across experiments, were actually higher when FBXL3 was overexpressed, with or without knockdown of CUL1 (XREF_SUPPLEMENTARY), indicating that the observed FBXL3 mediated reduction of ATXN3 levels occurs at the protein rather than transcriptional level, as expected."

reach
"Indeed, exogenous FBXL3 in SCA3 NPCs reduced the levels of both wild-type and pathogenic ATXN3 in a SCF dependent manner."
DNAJB1 affects ATXN3
| 6
DNAJB1 inhibits ATXN3. 6 / 7
| 6

reach
"Over-expression of the Drosophila DNAJB1 homolog, dHDJ1, suppressed polyQ Htt- and ATXN3 dependent toxicity [XREF_BIBR, XREF_BIBR], and the effect of dHDJ1 on ATXN3 mediated toxicity was enhanced by over-expression with wildtype Hsp70 but inhibited by co-expression with a dominant negative mutant of Hsp70."

reach
"An overexpression of HSP40 and HDJ -2 suppressed ataxin-3 and ataxin-1 aggregation in vitro [XREF_BIBR, XREF_BIBR], but not in huntingtin exon 1 overexpressing cell lines [XREF_BIBR]."

reach
"DNAJA1 effectively degrades huntington aggregates; DNAJB1 can degrade protein aggregates ataxin-3; DNAJB2 can inhibit the formation of huntington aggregates; DNAJB6 can inhibit the aggregation of Aβ 42 and α-synuclein; DNAJC5 can promote the release of TDP-43, τ protein, and α-synuclein into the extracellular space."

reach
"In support of this, it has been shown that overexpression of DNAJB1 restores the cellular degradation capacity [23] and suppresses aggregation of polyQ expanded ATXN3 [24]."

reach
"DNAJB1 was identified to suppress aggregate formation of ATXN3, but aggregation of the S256A mutant of ATXN3 could not be prevented by DNAJB1, it is still unclear whether DNAJB1 has preferential affinity for phosphorylated ATXN3."

reach
"For example, HSP40 and HSP70 were reported to localize in the intranuclear aggregates formed by mutant ataxin-3 and that overexpression of HSP40 reduces aggregation of truncated and full-length Atx3 XREF_BIBR."
CARMIL2 affects ATXN3
| 7
| 7

sparser
"Mutations in leucine-rich repeat kinase 2 (LRRK2) are strongly associated with late-onset autosomal dominant Parkinson’s disease."

sparser
"Autosomal dominant mutations in leucine-rich repeat kinase 2 ( LRRK2 ) are associated with Parkinson’s disease (PD)."

sparser
"For example, mutations in leucine-rich repeat kinase 2 (LRRK2) are associated with autosomal dominant PD [ xref ] and have been implicated in mitochondrial fragmentation and increased apoptotic rates relative to wild-type LRRK2 [ xref – xref ]."

sparser
"Leucine-rich repeat kinase 2 (LRRK2) has been associated with an autosomal dominant, late-onset form of familial Parkinson's disease (PD)."

sparser
"Mutations in leucine-rich repeat kinase 2 ( LRRK2 ) are associated with autosomal dominant PD, and several of these mutations have also been shown to increase the levels of reactive oxygen species in cells."

sparser
"Mutations in leucine-rich repeat kinase 2 (LRRK2) are strongly associated with late-onset autosomal dominant Parkinson's disease."

sparser
"Several different genes have been identified as susceptibility genes of familial PD(FDP)including alpha-synuclein gene (SNCA) and the leucine-rich repeat kinase 2 (LRRK2) mutations, which underlie autosomal dominant forms of PD, and mutations or multiplications of PINK1(PTEN-induced putative kinase 1), PARK7(Protein DJ1), and PARK2(Parkinson juvenile disease protein 2), with autosomal recessive forms of PD [ xref – xref ], especially in Chinese [ xref , xref ]."
ATXN7 affects ATXN3
| 2 5
ATXN7 binds ATXN3.
| 5
| 3

sparser
"Furthermore, we show that this binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA, similar to what we have shown previously for HTT (Krauss et al., xref ), induces translation in a CAG repeat length-dependent manner in vitro and in cell lines."

sparser
"To test if the flanking regions influence the binding of MID1 to CAG repeat mRNAs, we tested the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNAs."

sparser
"Furthermore, we show that functionally, in line with what we have previously observed for HTT, the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA induces protein synthesis in a repeat length-dependent manner."
| 2

sparser
"In this study we show that MID1 can also bind to the CAG repeat region of ATXN2, ATXN3 , and ATXN7 in vitro , suggesting that binding of MID1 to CAG repeats is not dependent on the flanking regions."

sparser
"We show that ATXN2, ATXN3 , and ATXN7 bind to MID1 in a CAG repeat length-dependent manner."
ATXN7 activates ATXN3.
| 2
ATXN7 activates ATXN3. 2 / 2
| 2

reach
"Ataxin gene variants in ATXN1, ATXN2, ATXN3, and ATXN7 cause SCA1, SCA2, SCA3, and SCA7, respectively."

reach
"XREF_BIBR SCA1, SCA2, Machado-Joseph or SCA3, SCA6, SCA7, SCA12, SCA17, and dentatorubral-pallidoluysian atrophy (DRPLA) are caused by (CAG) n repeat expansions in the ATXN1, ATXN2, ATXN3, CACNA1A, ATXN7, PPP2R2B, TBP, and ATN1 genes, respectively, and all lead to the expansion of a polyglutamine tract in the corresponding proteins."
ATXN3 affects RNF4
| 2
ATXN3 inhibits RNF4. 2 / 7
| 2

reach
"In particular, we suggest that ataxin-3 is a functional antagonist of RNF4 during the signaling and repair of DSBs where it acts as a SUMO activated DUB on at least one of the identified RNF4 substrates : MDC1."

reach
"The RNF4 dependent clearance of MDC1 is antagonised by ATXN3, a DUB that is rapidly recruited to DSBs by SUMO [25]."
ATXN3 affects NRL
| 7
| 7

sparser
"Missense mutations in human NRL have been associated with autosomal dominant retinitis pigmentosa."

sparser
"Missense mutations in NRL are associated with autosomal dominant retinitis pigmentosa; however, the phenotype associated with the loss of NRL function in humans has not been reported."

sparser
"Mutations in the human NRL gene are associated with autosomal dominant retinitis pigmentosa (adRP) and other retinopathies [ xref - xref ]."

sparser
"Missense mutations in the human NRL gene are associated with autosomal dominant retinitis pigmentosa, whereas the loss of its function leads to rodless retina in Nrl-knockout mice that exhibit enhanced S-cone function."

sparser
"In the human, missense mutations in NRL are associated with autosomal dominant retinitis pigmentosa [ xref , xref ]."

sparser
"Missense mutations in NRL are associated with autosomal dominant retinitis pigmentosa ( xref ; xref ; xref )."

sparser
"In humans, missense mutations in NRL are associated with autosomal dominant retinitis pigmentosa [ xref ]."
ATXN3 affects NCOR1
4 | 3
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
| 3

sparser
"Both normal (Q23) and expanded (Q70) human full-length Ataxin-3 physiologically interacted with HDAC3 and NCoR in a SCA3 rat cell line and human pons tissue."

sparser
"A direct interaction of ATXN3 with HDAC3 and NCoR has previously been established in SCA3 cell lines and human brain extracts yet with only normal (i.e. not expanded) ATXN3 being associated with increased deacetylase activity and repression of gene expression [ xref ]."

sparser
"Further, it was shown that both normal and expanded ataxin 3 physiologically interact with HDAC3 and NCoR in a SCA3 cell model and in human pons tissue; however, normal ataxin 3-containing protein complexes showed increased histone deacetylase activity, whereas polyglutamine-expanded ataxin 3-containing complexes had reduced deacetylase activity in target chromatin regions [ xref ]."

sparser
"Familial hemiplegic migraine type 2, an autosomal dominant form of migraine with aura, has been associated with four distinct mutations in the alpha2-subunit of the Na + ,K + -ATPase that is expressed primarily in astrocytes."

sparser
"In humans, mutations in the ATP1A2 gene result in familial hemiplegic migraines type II (FHM2), which are a rare autosomal dominant form of migraine with aura ( xref )."

sparser
"Familial hemiplegic migraine type 2 (FHM2) is an autosomal dominant form of migraine with aura that is caused by mutations of the α2-subunit of the Na,K-ATPase, an isoform almost exclusively expressed in astrocytes in the adult brain."

sparser
"Mutations in three genes encoding neural ion channels, CACNA1A, ATP1A2, and SCN1A, have been described in patients with familial hemiplegic migraine (FHM), a rare monogenic, autosomal dominant form of migraine with aura [ xref ]."

sparser
"FHM1 is a rare autosomal dominant form of migraine with aura, including transitory motor weakness."

sparser
"Familial hemiplegic migraine (FHM) is a rare autosomal dominant form of migraine with aura."

sparser
"As previously described for FHM3, FHM1 is a rare, autosomal dominant form of migraine with aura, characterized by recurrent attacks of disabling headache and, in some cases, progressive cerebellar atrophy."
ATXN3 affects LRRK2
| 7
| 7

sparser
"This observation is consistent with finding of association of human LRRK2 mutations with autosomal dominant form of PD."

sparser
"Mutations in the protein kinase LRRK2 are associated with rare forms of autosomal dominant Parkinson's disease and patients carrying LRRK2 mutations present with symptoms resembling idiopathic Parkinson's disease xref , xref ."

sparser
"Mutations in LRRK2 are associated with both autosomal dominant familial and sporadic forms of PD."

sparser
"Mutations in leucine rich repeat kinase 2 (LRRK2), which are associated with autosomal dominant Parkinson’s disease, elicit progressive dendrite degeneration in neurons."

sparser
"LRRK2 mutations are also associated with autosomal dominant forms of PD ( xref )."

sparser
"Mutations in LRRK2 are associated with autosomal dominant PD, and common genetic variants are associated with an increased risk of developing sporadic PD [ xref ]."

sparser
"Autosomal dominant mutations in LRRK2 are associated with both familial and late-onset PD and patients have clinical symptoms and pathology typical of sporadic PD ( xref )."
ATXN3 affects LGI1
| 7
| 7

sparser
"Mutations in the LGI1 gene are associated with the autosomal dominant lateral temporal epilepsy (ADLTE) syndrome ( xref ), also known as autosomal dominant partial epilepsy with auditory features ( xref )."

sparser
"Mutations in the LGI1 gene are associated with a form of human autosomal dominant epilepsy ( xref ; xref )."

sparser
"LGI1 cysteine mutations have been associated with autosomal dominant lateral temporal lobe epilepsy [ xref ]."

sparser
"Prevailing evidence demonstrates that Lgi1 is tightly associated with autosomal dominant lateral temporal lobe epilepsy (ADLTE; Senechal et al., xref ; Sirerol-Piquer et al., xref ; Head et al., xref )."

sparser
"LGI1 is a secreted neuronal protein that interacts with presynaptic and postsynaptic receptors, and mutations of LGI1 have been associated with the syndrome of autosomal dominant lateral temporal lobe epilepsy [ xref , xref ]."

sparser
"LGI2 mutations have not been associated with human epilepsy, but autosomal dominant lateral temporal lobe epilepsy (ADLTE) is associated with mutations in LGI1, which interacts with the same ADAM-family receptors."
| PMC

sparser
"Interestingly, LGI1 mutations have been associated with an autosomal dominant lateral temporal lobe epilepsy manifesting often with auditory features ( xref )."
| 7

sparser
"The interaction between ataxin-3 and these E3s may serve several purposes."

sparser
"In addition to CHIP, Ataxin-3 also interacts with another E3 ligase, which is parkin ( xref ; xref )."

sparser
"Ataxin-3 interacts with two E3 ligases that are essential for maintaining normal cellular hemostasis, namely C terminus of Hsc70-interacting protein (CHIP) and parkin."

sparser
"Interestingly, ataxin-3 associates with a putative ubiquitin ligase, the potassium channel tetramerization domain-10 (KCTD10) protein ( xref )."

sparser
"Early studies hinted that the U -box E3-Ub ligase CHIP might be one such E3 that interacts with ataxin-3."

sparser
"The regulation of ataxin-3 activity through ubiquitination might depend on the interaction of ataxin-3 with several E3 ubiquitin ligases (Durcan and Fon, xref ), such as the C-terminus of 70 kDa heat-shock protein (Hsp70)-interacting protein (CHIP), parkin, and E4B (Figure xref ), since all were shown to promote ataxin-3 ubiquitination and regulate its degradation by the proteasome (Matsumoto et al., xref ; Jana et al., xref ; Miller et al., xref )."

sparser
"After all, there are reports of ubiquitin ligases that interact with ataxin-3, increasing its ubiquitination and its proteasomal turnover ( xref ; xref ; xref ; xref ; xref )."
ATXN3 affects CARMIL2
| 7
| 7

sparser
"Mutations in leucine-rich repeat kinase 2 (LRRK2) are strongly associated with late-onset autosomal dominant Parkinson’s disease."

sparser
"Autosomal dominant mutations in leucine-rich repeat kinase 2 ( LRRK2 ) are associated with Parkinson’s disease (PD)."

sparser
"For example, mutations in leucine-rich repeat kinase 2 (LRRK2) are associated with autosomal dominant PD [ xref ] and have been implicated in mitochondrial fragmentation and increased apoptotic rates relative to wild-type LRRK2 [ xref – xref ]."

sparser
"Leucine-rich repeat kinase 2 (LRRK2) has been associated with an autosomal dominant, late-onset form of familial Parkinson's disease (PD)."

sparser
"Mutations in leucine-rich repeat kinase 2 ( LRRK2 ) are associated with autosomal dominant PD, and several of these mutations have also been shown to increase the levels of reactive oxygen species in cells."

sparser
"Mutations in leucine-rich repeat kinase 2 (LRRK2) are strongly associated with late-onset autosomal dominant Parkinson's disease."

sparser
"Several different genes have been identified as susceptibility genes of familial PD(FDP)including alpha-synuclein gene (SNCA) and the leucine-rich repeat kinase 2 (LRRK2) mutations, which underlie autosomal dominant forms of PD, and mutations or multiplications of PINK1(PTEN-induced putative kinase 1), PARK7(Protein DJ1), and PARK2(Parkinson juvenile disease protein 2), with autosomal recessive forms of PD [ xref – xref ], especially in Chinese [ xref , xref ]."
ATXN3 affects ATXN7
| 2 5
ATXN3 binds ATXN7.
| 5
| 3

sparser
"Furthermore, we show that this binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA, similar to what we have shown previously for HTT (Krauss et al., xref ), induces translation in a CAG repeat length-dependent manner in vitro and in cell lines."

sparser
"To test if the flanking regions influence the binding of MID1 to CAG repeat mRNAs, we tested the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNAs."

sparser
"Furthermore, we show that functionally, in line with what we have previously observed for HTT, the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA induces protein synthesis in a repeat length-dependent manner."
| 2

sparser
"In this study we show that MID1 can also bind to the CAG repeat region of ATXN2, ATXN3 , and ATXN7 in vitro , suggesting that binding of MID1 to CAG repeats is not dependent on the flanking regions."

sparser
"We show that ATXN2, ATXN3 , and ATXN7 bind to MID1 in a CAG repeat length-dependent manner."
ATXN3 activates ATXN7.
| 2
ATXN3 activates ATXN7. 2 / 2
| 2

reach
"XREF_BIBR SCA1, SCA2, Machado-Joseph or SCA3, SCA6, SCA7, SCA12, SCA17, and dentatorubral-pallidoluysian atrophy (DRPLA) are caused by (CAG) n repeat expansions in the ATXN1, ATXN2, ATXN3, CACNA1A, ATXN7, PPP2R2B, TBP, and ATN1 genes, respectively, and all lead to the expansion of a polyglutamine tract in the corresponding proteins."

reach
"Ataxin gene variants in ATXN1, ATXN2, ATXN3, and ATXN7 cause SCA1, SCA2, SCA3, and SCA7, respectively."

sparser
"Regulated association of atx3 with the ER membrane."

sparser
"The simplest explanation of these observations is that a small fraction of atx3 is transiently associated with the ER membrane via interactions with p97 and VIMP. atx3 may shuttle on and off p97 to promote PAD, a process that is linked to the ATPase cycle of p97 and to the subsequent delivery of substrates to the proteasome."

sparser
"We demonstrate that atx3 transiently associates with the ER membrane via p97 and the recently identified Derlin–VIMP complex, and its release from the membrane appears to be governed by both the p97 ATPase cycle and its own deubiquitinating activity."

sparser
"Together, these data suggest that a fraction of atx3 is associated with the ER membranes via interactions with components of the retrotranslocation machinery, including Derlin-1, VIMP, p97, and an ER-specific ubiquitin ligase."

reach
"Using these models, we discovered that chemical modulation of the UPR ER reduced neurodegeneration and warrants investigation in mammalian models of MJD."

reach
"Gp78, an ER associated E3, promotes SOD1 and ataxin-3 degradation."
Calcium(2+) affects ATXN3
| 6
| 6

reach
"It was discovered that inhibition of Ca 2+ -dependent protease calpain suppressed aggregation of polyglutamine expanded Atxn3 in transfected cells [XREF_BIBR]."

reach
"It was discovered that inhibition of Ca 2+ -dependent protease calpain suppressed aggregation of polyglutamine expanded Atxn3 in transfected cells [XREF_BIBR]."

reach
"The authors hypothesized that calcium dependent activation of proteases causes the cleavage of ATXN3 leading to protein aggregation as a result."

reach
"Most of these findings parallel our previous studies of Ca 2+ signaling in HD and SCA3 mouse models and indicate that deranged Ca 2+ signaling contributes to pathogenesis of at least these three polyglutamine-expansion disorders."

reach
"It was discovered that inhibition of Ca 2+ -dependent protease calpain suppressed aggregation of polyglutamine expanded Atxn3 in transfected cells."

reach
"Administration of an activator of calcium activated potassium channels, SKA-31, partially corrects abnormal Purkinje cell firing and improves motor function in SCA3 mice."
WFS1 affects ATXN3
| 6
| 6

sparser
"Lys836Asn is a novel mutation in WFS1 that is associated with autosomal dominant optic neuropathy and finally a severe to profound hearing loss with relatively flat audiograms."

sparser
"For instance, SLC26A4, associated with autosomal recessive NSHL xref and Pendred syndrome, is a gene coding for pendrin, a chloride/iodide transporter; COCH, responsible for autosomal dominant non syndromic post-lingual with a progressive onset in adulthood xref , encodes for cochlin, a component of the extracellular matrix of the inner ear; POU3F4, responsible for an X-linked non syndromic progressive and profound sensorineural hearing loss xref , encodes for a transcription factor; while WFS1 associated with autosomal recessive Wolfram syndrome and autosomal dominant low frequency NSHL xref , xref , is a gene coding for the glycoprotein wolframin."

sparser
"The present report describes the phenotype of a third family with autosomal dominant optic neuropathy and deafness that is associated with a novel missense mutation in WFS1 ."

sparser
"The hearing loss associated with autosomal dominant mutations in WFS1 is primarily bilateral and symmetric."

sparser
"A rare autosomal dominant form of WFS1 also exists xref ."

sparser
"Lys836Asn) in exon 8 of WFS1 that is associated with autosomal dominant optic neuropathy and deafness."
UBL affects ATXN3
| 6
ATXN3 binds UBL. 4 / 4
| 4

sparser
"Ataxin-3 interacts with the UBL domain of HHR23 proteins."

sparser
"The fact that ATXN3 interacts with the UBL domain of HHR23 proteins and the recent finding that NEDD8 is present in ubiquitylated inclusions in the brain of MJD patients led us to investigate whether[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Overall, these data indicate that phosphorylation dampens the Ubl domain interaction with the UIM-containing protein ataxin-3 through loss of interactions with residues near the Ser-65 site."

sparser
"The interactions of ataxin-3 with the Ubl P37L and pUbl P37L domains were also examined to identify how a Parkinson's disease–substituted parkin combined with phosphorylation might further alter this interaction ( xref B )."
ATXN3 binds PRKN, Dnmt1, and UBL. 2 / 2
| 2

sparser
"For example, the interaction of the parkin Ubl domain with the UIM regions of the deubiquitinating protein ataxin-3 is required for the unique ubiquitin-editing role of ataxin-3 ( xref , xref )."

sparser
"The observation that the three UIM sites in ataxin-3 bind equally to a single site of the parkin Ubl domain suggests a multi- or polyvalent ligand binding mode."
UBE2W affects ATXN3
| 4 2
UBE2W ubiquitinates ATXN3. 6 / 6
| 4 2

reach
"The presence of CHIP markedly enhanced Ube2w mediated ubiquitination of ataxin-3 in a manner requiring full length CHIP (XREF_SUPPLEMENTARY)."

sparser
"In some cases E2-mediated ubiquitination of UIM proteins can occur independent of E3s ( xref ), thus we tested whether CHIP was necessary for Ube2w-dependent ubiquitination of ataxin-3."

sparser
"Consistent with UbcH5 and Ube2w dictating substrate residue specificity, UbcH5 ubiquitinated GST ATXN3, but not KO ATXN3 ( xref ) and Ube2w ubiquitinated KO ATXN3, but not GST ATXN3 in the absence of CHIP ( xref )."

reach
"In a CHIP ubiquitination reaction, Ube2w monoubiquitinated ataxin-3 more rapidly and robustly than did a second E2, UbcH5c (XREF_SUPPLEMENTARY)."

reach
"Ube2w, on the other hand, is able to successfully ubiquitylate the N-terminus of a lysine less version of Ataxin-3 and Tau [XREF_BIBR]."

reach
"In some cases E2 mediated ubiquitination of UIM proteins can occur independent of E3s, thus we tested whether CHIP was necessary for Ube2w dependent ubiquitination of ataxin-3."
TGM6 affects ATXN3
| 4 2
| 4 2

sparser
"To further examine the possible interaction between ataxin-3 and TG6, we co-transfected HEK293 cells with Myc-tagged TG6 and EGFP or EGFP-tagged ataxin-3 (20Q/70Q) expression plasmids, respectively."

reach
"Equal amounts of proteins in each fraction were analyzed by immunoblotting.We first analyzed the interaction between TG6 and SCA3 associated polyQ protein ataxin-3 (20Q/70Q) by GST pull-down assay and[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"We thus demonstrated specific binding between TG6 and ataxin-3 in vivo."

reach
"These data suggest that both normal and expanded ataxin-3 interact with WT TG6 in vitro."

reach
"To further examine the possible interaction between ataxin-3 and TG6, we co-transfected HEK293 cells with Myc tagged TG6 and EGFP or EGFP tagged ataxin-3 (20Q/70Q) expression plasmids, respectively."

sparser
"These data suggest that both normal and expanded ataxin-3 interact with WT TG6 in vitro."
N-VCP affects ATXN3
| 6
N-VCP inhibits ATXN3.
| 2
N-VCP inhibits ATXN3. 2 / 2
| 2

reach
"Collectively, these findings indicate that N-VCP suppresses lethality in the ‘weaker’ SCA3 line."

reach
"Since these blots suggested that the presence of N-VCP reduces aggregated species of pathogenic ataxin-3, we sought to confirm these results through the utilization of filter-trap assays."
N-VCP increases the amount of ATXN3.
| 2
N-VCP increases the amount of ATXN3. 2 / 2
| 2

reach
"We conclude that N-VCP reduces the interaction of ataxin-3 with endogenous VCP and leads to lower levels of aggregated, pathogenic ataxin-3."

reach
"The presence of N-VCP seemed to increase the amount of SDS-soluble ataxin-3 (Fig. 6A, green outlines)."
N-VCP decreases the amount of ATXN3.
| 2
N-VCP decreases the amount of ATXN3. 2 / 2
| 2

reach
"Lysates from flies expressing pathogenic ataxin-3 alone or with N-VCP showed that the presence of the truncated protein significantly reduced the amount of aggregated ataxin-3 trapped on the membrane (Fig. 6B)."

reach
"From these data we conclude that N-VCP leads to reduced levels of aggregated, pathogenic ataxin-3."
IMPDH1 affects ATXN3
| 6
| 6

sparser
"Mutations in IMPDH1 cause the RP10 form of autosomal dominant retinitis pigmentosa, and are a rare cause of Leber congenital amaurosis."

sparser
"Inosine monophosphate dehydrogenase I (IMPDH1) has been identified as the gene responsible for the RP10 form of autosomal dominant retinitis pigmentosa (adRP)."
| PMC

sparser
"The gene that is responsible for the RP10 form of autosomal dominant retinitis pigmentosa (adRP) has been mapped to human chromosome 7q31.1, and accounts for 5–10% of all RP cases in the US and Europe."
| PMC

sparser
"A novel IMPDH1 gene mutation (Arg231Pro) was associated with a severe form of autosomal dominant retinitis pigmentosa."

sparser
"Mutations in IMPDH1 cause the RP10 form of autosomal dominant retinitis pigmentosa (adRP) and are a rare cause of dominant Leber congenital amaurosis (LCA)."

sparser
"Two substitution mutations in the cystathionine-β-synthase (CBS) subdomain of IMPDH1, which are associated with human autosomal dominant retinitis pigmentosa, impair nucleic acid binding but not enzymatic activity xref , suggesting that the inability of mutant IMPDH to bind to DNA contributes to human disease."
HSPA affects ATXN3
| 6
HSPA binds ATXN3.
| 3
| 3

reach
"A similar model of CHIP and Hsp70 interaction with HTT and ATXN3 was proposed, although no single modified residue was identified as a recognition site."

reach
"This possibly reflects a more transient interaction between the E3-ligase and ataxin-3, compared to the interaction between the chaperone Hsp70 and ataxin-3."

reach
"Next, we tested whether HSJ1a, CHIP, HSP70 and Atx3 can form a complex in cells."
HSPA activates ATXN3.
| 3
HSPA activates ATXN3. 3 / 3
| 3

reach
"It was previously reported that overexpression of CHIP increases the ubiquitination and degradation rates of polyQ expanded Atx3, which is also enhanced by HSP70 XREF_BIBR."

reach
"Another example of the gene-to-drug transition is the rescue of Atx3 and alpha-Syn toxicity by Hsp70."

reach
"The increased turnover of both ataxin-3 variants induced by Hsp70 and the large number of Hsp70 family chaperones in the MS analysis suggests that a subpopulation of ataxin-3 adopts a non native confo[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
EGFP affects ATXN3
| 6
| 6

sparser
"However, there was some nuclear GFP fluorescence not co-labeled with the polyglutamine antibody, suggesting partial degradation of the EGFP-ataxin-3 fusion protein, resulting in some GFP separated from the polyQ tract."

sparser
"Whilst it may be questioned whether the treatment with BLD-2736 may have instead decreased full-length ataxin-3 through effects on the expression of EGFP-ataxin-3 via an effect on transcription, we previously demonstrated that treatment with calpeptin decreased the presence of full length human ataxin-3 protein, without altering the levels of human ataxin-3 mRNA, and that this effect was prevented by cotreatment with the autophagy inhibitor, chloroquine [ xref ]."
| PMC

sparser
"EGFP ataxin-3 84Q larvae treated with chloroquine did have statistically higher LC3II/GAPDH compared to those treated with vehicle alone, suggesting that autophagic flux occurs at baseline in EGFP-ataxin-3 84Q larvae ( p = 0.011)."
| PMC

sparser
"In contrast, we did not see any nuclear mislocalization or aggregation of expanded polyQ human ataxin-3 in the neurons cultured from our EGFP-ataxin-3 zebrafish larva ( xref )."

sparser
"However, we did find aggregates of mCherry protein that did not localize with EGFP-ataxin-3."

sparser
"Nevertheless, these cell culture findings were consistent with what was seen in whole mount samples from the transgenic EGFP-ataxin-3 zebrafish larvae."
DNM2 affects ATXN3
1 | 5
1 | 5

sparser
"Heterozygous mutations in DNM2 are associated with two distinct neuromuscular disorders, Charcot-Marie-Tooth Disease (CMT) and autosomal dominant centronuclear myopathy (CNM)."

sparser
"Dominant mutations in DNM2 (dynamin-2) are associated with a common congenital myopathy subtype called autosomal dominant centronuclear myopathy."

sparser
"Mutations to the dynamin 2 gene ( DNM2 ) were recently associated with the autosomal dominant form of CNM while X-linked CNMs were associated with mutations in DNM2 , AMPH (amphiphysin), MTM1 (myotubularin) and most recently MTMR14 (myotubularin-related protein-14) genes (Laporte et al., xref , xref ; Jungbluth et al., xref ; Romero, xref ; Romero and Bitoun, xref )."

sparser
"Mutations in DNM2 are associated with autosomal dominant centronuclear myopathy (ADCNM) and intermediate CMT [ xref ]."

No evidence text available

sparser
"Autosomal dominant centronuclear myopathy is classically associated with mutations in DNM2 which codes for dynamin 2 [ xref ], a GTPase that is involved in the mechanisms of endocytosis and transport of organelles along the network of microtubules, and also plays a key role in centrosome formation [ xref ]."
ATXN3 affects polyQ
| 6
ATXN3 inhibits polyQ. 6 / 6
| 6

reach
"Based on our collective findings, ataxin-3 suppresses polyQ dependent toxicity not by helping discard toxic protein species, but by ultimately decreasing aggregates."

reach
"Additionally, ataxin-3 suppresses polyQ dependent degeneration without eliminating the polyQ species, based on western blots, suggesting that the elimination of the disease causing agent by the proteasome or through autophagy is not part of the protective aspect of ataxin-3 in Drosophila; this conclusion is also supported by genetic experiments."

reach
"Altogether, our results propose a model whereby ataxin-3 increases DnaJ-1 levels in a manner that depends on the catalytic activity of this DUB and on its interaction with Rad23, and that DnaJ-1, acti[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Here, we investigate ataxin-3 partners and PQC pathways in an effort to understand how ataxin-3 suppresses polyQ dependent toxicity in flies."

reach
"Normal ataxin-3 suppresses polyQ neurodegeneration in Drosophila by an ubiquitin associated mechanism [19]."

reach
"Together, our findings are indicative of a model in which ataxin-3 suppresses polyQ dependent degeneration by increasing the levels of DnaJ-1, which in turn functions with stress inducible chaperones to protect neurons by decreasing polyQ aggregates."
ATXN3 affects mutations
| 6
ATXN3 activates mutations. 6 / 6
| 6

sparser
"In humans, enhanced signalling results from autosomal dominant activating NPR2 mutations causing bony overgrowth and tall stature [ 41 ] (MIM# 615923 )."

sparser
"In humans, enhanced signalling results from autosomal dominant activating NPR2 mutations causing bony overgrowth and tall stature [ xref ] (MIM#)."

sparser
"Autosomal dominant activating mutations in PTPN11 fuel excess RAS/MAPK signaling that drives certain human RASopathies and cancers xref , xref ."

sparser
"Autosomal dominant activating mutations in PCSK-9 cause familial hypercholesterolaemia whereas inactivating mutations in man reduce LDL cholesterol (LDL-C) and are associated with a decreased lifetime risk of cardiovascular events."

sparser
"Notably, increased risk of IPMN has been described in McCune-Albright syndrome, which is caused by post-zygotic mosaic autosomal dominant activating mutations of GNAS . xref , xref Oncogenic GNAS has also been identified in other tumor types including those from the pituitary, liver, and colon. xref , xref , xref "

sparser
"However, aberrant or prolonged inflammasome activation can be pathogenic, as is seen in sterile inflammatory disorders such as gout and pseudogout, and in autoinflammatory diseases caused by autosomal dominant activating mutations in inflammasome components, such as cryopyrin-associated periodic syndromes (CAPS) with mutations in NLRP3 and Familial Mediterranean Fever with mutations in MEFV ( xref , xref )."

reach
"Ataxin-3 promotes testicular cancer cell proliferation by inhibiting anti-oncogene PTEN."

reach
"We conjectured that upregulated HRH1 and ATXN3 were targeted by both piRNAs and miRNAs, and along with BRAF and CCND1 might induce cell proliferation in GBM through G-protein-coupled receptor or Akt signalling pathways due to downregulation of the respective targeting small RNAs."

reach
"Moreover, various gain/loss functional assays were performed to indicate that ATXN3 overexpression enhanced ATC cell proliferation and metastasis."

reach
"We also found that ATXN3 and eukaryotic translation initiation factor 5A2 (EIF5A2) protein levels in ATC tissues are positively correlated, and ATXN3 promotes the proliferation and metastasis of ATC cells through EIF5A2."

reach
"Functionally, Ataxin-3 overexpression promoted cell proliferation, and Ataxin-3 knockdown inhibited cell proliferation."

reach
"In addition, ATXN3 depletion significantly decreased PC cell proliferation, invasion and stem-like properties."
ATXN3 affects WFS1
| 6
| 6

sparser
"Lys836Asn is a novel mutation in WFS1 that is associated with autosomal dominant optic neuropathy and finally a severe to profound hearing loss with relatively flat audiograms."

sparser
"For instance, SLC26A4, associated with autosomal recessive NSHL xref and Pendred syndrome, is a gene coding for pendrin, a chloride/iodide transporter; COCH, responsible for autosomal dominant non syndromic post-lingual with a progressive onset in adulthood xref , encodes for cochlin, a component of the extracellular matrix of the inner ear; POU3F4, responsible for an X-linked non syndromic progressive and profound sensorineural hearing loss xref , encodes for a transcription factor; while WFS1 associated with autosomal recessive Wolfram syndrome and autosomal dominant low frequency NSHL xref , xref , is a gene coding for the glycoprotein wolframin."

sparser
"The present report describes the phenotype of a third family with autosomal dominant optic neuropathy and deafness that is associated with a novel missense mutation in WFS1 ."

sparser
"The hearing loss associated with autosomal dominant mutations in WFS1 is primarily bilateral and symmetric."

sparser
"A rare autosomal dominant form of WFS1 also exists xref ."

sparser
"Lys836Asn) in exon 8 of WFS1 that is associated with autosomal dominant optic neuropathy and deafness."
ATXN3 affects UBL
| 6
ATXN3 binds UBL. 4 / 4
| 4

sparser
"Ataxin-3 interacts with the UBL domain of HHR23 proteins."

sparser
"The fact that ATXN3 interacts with the UBL domain of HHR23 proteins and the recent finding that NEDD8 is present in ubiquitylated inclusions in the brain of MJD patients led us to investigate whether[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Overall, these data indicate that phosphorylation dampens the Ubl domain interaction with the UIM-containing protein ataxin-3 through loss of interactions with residues near the Ser-65 site."

sparser
"The interactions of ataxin-3 with the Ubl P37L and pUbl P37L domains were also examined to identify how a Parkinson's disease–substituted parkin combined with phosphorylation might further alter this interaction ( xref B )."
ATXN3 binds PRKN, Dnmt1, and UBL. 2 / 2
| 2

sparser
"For example, the interaction of the parkin Ubl domain with the UIM regions of the deubiquitinating protein ataxin-3 is required for the unique ubiquitin-editing role of ataxin-3 ( xref , xref )."

sparser
"The observation that the three UIM sites in ataxin-3 bind equally to a single site of the parkin Ubl domain suggests a multi- or polyvalent ligand binding mode."
ATXN3 affects TGM6
| 4 2
| 4 2

sparser
"To further examine the possible interaction between ataxin-3 and TG6, we co-transfected HEK293 cells with Myc-tagged TG6 and EGFP or EGFP-tagged ataxin-3 (20Q/70Q) expression plasmids, respectively."

reach
"Equal amounts of proteins in each fraction were analyzed by immunoblotting.We first analyzed the interaction between TG6 and SCA3 associated polyQ protein ataxin-3 (20Q/70Q) by GST pull-down assay and[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"We thus demonstrated specific binding between TG6 and ataxin-3 in vivo."

reach
"These data suggest that both normal and expanded ataxin-3 interact with WT TG6 in vitro."

reach
"To further examine the possible interaction between ataxin-3 and TG6, we co-transfected HEK293 cells with Myc tagged TG6 and EGFP or EGFP tagged ataxin-3 (20Q/70Q) expression plasmids, respectively."

sparser
"These data suggest that both normal and expanded ataxin-3 interact with WT TG6 in vitro."
ATXN3 affects IMPDH1
| 6
| 6

sparser
"Mutations in IMPDH1 cause the RP10 form of autosomal dominant retinitis pigmentosa, and are a rare cause of Leber congenital amaurosis."

sparser
"Inosine monophosphate dehydrogenase I (IMPDH1) has been identified as the gene responsible for the RP10 form of autosomal dominant retinitis pigmentosa (adRP)."
| PMC

sparser
"The gene that is responsible for the RP10 form of autosomal dominant retinitis pigmentosa (adRP) has been mapped to human chromosome 7q31.1, and accounts for 5–10% of all RP cases in the US and Europe."
| PMC

sparser
"A novel IMPDH1 gene mutation (Arg231Pro) was associated with a severe form of autosomal dominant retinitis pigmentosa."

sparser
"Mutations in IMPDH1 cause the RP10 form of autosomal dominant retinitis pigmentosa (adRP) and are a rare cause of dominant Leber congenital amaurosis (LCA)."

sparser
"Two substitution mutations in the cystathionine-β-synthase (CBS) subdomain of IMPDH1, which are associated with human autosomal dominant retinitis pigmentosa, impair nucleic acid binding but not enzymatic activity xref , suggesting that the inability of mutant IMPDH to bind to DNA contributes to human disease."
ATXN3 affects EGFP
| 6
| 6

sparser
"However, there was some nuclear GFP fluorescence not co-labeled with the polyglutamine antibody, suggesting partial degradation of the EGFP-ataxin-3 fusion protein, resulting in some GFP separated from the polyQ tract."

sparser
"Whilst it may be questioned whether the treatment with BLD-2736 may have instead decreased full-length ataxin-3 through effects on the expression of EGFP-ataxin-3 via an effect on transcription, we previously demonstrated that treatment with calpeptin decreased the presence of full length human ataxin-3 protein, without altering the levels of human ataxin-3 mRNA, and that this effect was prevented by cotreatment with the autophagy inhibitor, chloroquine [ xref ]."
| PMC

sparser
"EGFP ataxin-3 84Q larvae treated with chloroquine did have statistically higher LC3II/GAPDH compared to those treated with vehicle alone, suggesting that autophagic flux occurs at baseline in EGFP-ataxin-3 84Q larvae ( p = 0.011)."
| PMC

sparser
"In contrast, we did not see any nuclear mislocalization or aggregation of expanded polyQ human ataxin-3 in the neurons cultured from our EGFP-ataxin-3 zebrafish larva ( xref )."

sparser
"However, we did find aggregates of mCherry protein that did not localize with EGFP-ataxin-3."

sparser
"Nevertheless, these cell culture findings were consistent with what was seen in whole mount samples from the transgenic EGFP-ataxin-3 zebrafish larvae."
ATXN3 affects DNM2
1 | 5
1 | 5

sparser
"Heterozygous mutations in DNM2 are associated with two distinct neuromuscular disorders, Charcot-Marie-Tooth Disease (CMT) and autosomal dominant centronuclear myopathy (CNM)."

sparser
"Dominant mutations in DNM2 (dynamin-2) are associated with a common congenital myopathy subtype called autosomal dominant centronuclear myopathy."

sparser
"Mutations to the dynamin 2 gene ( DNM2 ) were recently associated with the autosomal dominant form of CNM while X-linked CNMs were associated with mutations in DNM2 , AMPH (amphiphysin), MTM1 (myotubularin) and most recently MTMR14 (myotubularin-related protein-14) genes (Laporte et al., xref , xref ; Jungbluth et al., xref ; Romero, xref ; Romero and Bitoun, xref )."

sparser
"Mutations in DNM2 are associated with autosomal dominant centronuclear myopathy (ADCNM) and intermediate CMT [ xref ]."

No evidence text available

sparser
"Autosomal dominant centronuclear myopathy is classically associated with mutations in DNM2 which codes for dynamin 2 [ xref ], a GTPase that is involved in the mechanisms of endocytosis and transport of organelles along the network of microtubules, and also plays a key role in centrosome formation [ xref ]."
ATXN3 affects CASP3
| 3 3
ATXN3 binds CASP3.
| 3
| 3

sparser
"The most common configuration for the normal allele is (CAG) 8 CAA(-CAG) 4 CAA(CAG) 8 , xref whereas the most common SCA2 pathogenic allele contains 37 uninterrupted CAG trip-lets. xref The prevalence of SCA2 is 1–2/100,000, similar to that of another form of autosomal dominant ataxia, SCA1. xref However, significant geographic and ethnic variations exist, with the prevalence reaching 41/100,000 in the Holguin region of Cuba due to a possible founder effect. xref Recently, intermediate-length ATXN2 repeat expansions (27–33 triplets), mostly interrupted by 1 to 3 CAA triplets, have been associated with an increased risk for amyotrophic lateral sclerosis (ALS). xref "

sparser
"Moreover, up-regulation of atx2 , the Drosophila ortholog of the human gene that causes SCA2 disease, has been shown to enhance the toxicity of human disease forms of SCA1 and SCA3 in flies [ xref ]."

sparser
"These SCAs (SCA1SCA3, SCA6, SCA7, and SCA17) are clinically characterized by a progressive ataxia with cerebellar degeneration that in most of these SCAs consists of severe degeneration and loss of Purkinje cells, the major integrative neuron of the cerebellar cortex ( xref ; xref )."
ATXN3 activates CASP3.
| 3
ATXN3 activates CASP3. 3 / 3
| 3

reach
"Ataxin gene variants in ATXN1, ATXN2, ATXN3, and ATXN7 cause SCA1, SCA2, SCA3, and SCA7, respectively."

reach
"XREF_BIBR SCA1, SCA2, Machado-Joseph or SCA3, SCA6, SCA7, SCA12, SCA17, and dentatorubral-pallidoluysian atrophy (DRPLA) are caused by (CAG) n repeat expansions in the ATXN1, ATXN2, ATXN3, CACNA1A, ATXN7, PPP2R2B, TBP, and ATN1 genes, respectively, and all lead to the expansion of a polyglutamine tract in the corresponding proteins."

reach
"At least 6 autosomal dominant forms of spinocerebellar ataxia (SCA1, 2, 3, 6, 7, and 17) are caused by CAG repeat expansions, the most common of these being SCA3, or Machado-Joseph disease (MJD)."
ATXN3 affects ANKH
| 6
| 6

sparser
"Mutations for the autosomal dominant form of CMD have been identified in of progressive ankylosis ( ANKH ) gene and for a recessive form in Connexin 43 ( Cx43 ) [ xref – xref ]."

sparser
"It will be interesting to study any discernible clinical differences of the recessive CX43 form of CMD to the autosomal dominant form caused by ANKH mutations once more patients with CX43 mutations have been identified for this very rare craniotubular disorder."

sparser
"Mutations for the autosomal dominant form of CMD have been identified in the ANKH gene and are mostly one amino acid deletions or insertions that cluster in the C terminus ( xref , xref )."

sparser
"The commonest of these is the autosomal dominant form of craniometaphyseal dysplasia, while the others which are well known include Pyle disease, and van Buchem disease."

sparser
"In particular, Pyle disease has been confused mainly with the autosomal dominant form of craniometaphyseal dysplasia, which is milder than the recessive type [ xref ]."

sparser
"Mutations located in cytoplasmic domains close to the C terminus of the human homolog of the Ank gene ( ANKH ) were identified for the autosomal dominant form of craniometaphyseal dysplasia (CMD) ( xref ; xref )."
UbS2 affects ATXN3
| 5
UbS2 activates ATXN3.
| 3
UbS2 activates ATXN3. 3 / 3
| 3

reach
"UbS2 mediates the interaction of ataxin-3 with the proteasome associated proteins Rad23A and Rad23B XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR."

reach
"CHX based analyses are consistent with steady-state examinations, indicating that UbS2 inhibits ataxin-3 degradation in cells, while VCP binding does not appear to have a significant effect."

reach
"Just as we observed with normal ataxin-3, mutating UbS2 enhances the turnover rate of pathogenic ataxin-3 in cells (XREF_FIG)."
UbS2 inhibits ATXN3.
| 2
UbS2 inhibits ATXN3. 2 / 2
| 2

reach
"Mutating UbS2 does not abrogate the catalytic activity of ataxin-3 23 or affect its subcellular localization (XREF_SUPPLEMENTARY)."

reach
"Collectively, these results from mammalian cell culture indicate that UbS2 inhibits the proteasomal turnover of non pathogenic ataxin-3."
USP19 affects ATXN3
| 5
USP19 activates ATXN3. 5 / 5
| 5

reach
"We next examined the effect of usp19 silencing on the protein levels of Atx3 100Q and Htt-N552 100Q, and observed that knockdown of USP19 significantly reduced the protein levels of Atx3 100Q (XREF_FIG) and Htt-N552 100Q (XREF_FIG) both in HEK 293T cells and in human retinal pigment epithelial (RPE1) cells (XREF_SUPPLEMENTARY)."

reach
"Beclin-1 stabilization is also promoted by USP19 and ataxin 3, which specifically removes K11- and K48-ubiquitin chain from Belcin-1, respectively [38, 39]."

reach
"The major finding of this work is that cytoplasmic USP19 can promote aggregation of the polyQ expanded Atx3 and Htt proteins by up-regulating their protein levels."

reach
"The regulatory function of USP19 was recently confirmed in a study demonstrating that USP19 interacts directly with chaperone Hsp90 and upregulates the aggregation of poly-Q containing the proteins Ataxin-3 and Huntingtin, which causes spinocerebellar ataxia type-3 and Huntington’s disease, respectively [90]."

reach
"Cytoplasmic Ubiquitin Specific Protease 19 (USP19) Modulates Aggregation of Polyglutamine Expanded Ataxin-3 and Huntingtin through the HSP90 Chaperone."
Tubulin affects ATXN3
| 2
| 2

sparser
"In fact, ataxin-3 is able to interact with tubulin through its JD domain (Figure xref ), with nM affinity (Mazzucchelli et al., xref ), which supports a role in cell structure."

sparser
"Previous reports showed that ATXN3 interacts with tubulin and microtubules ( xref ) and also with HDAC6 and dynein to promote aggresome transport to the microtubule organization center (MTOC) ( xref )."
TNF affects ATXN3
| 5
| 5

sparser
"It is a rare autosomal dominant disease and strongly associated with heterozygous mutations in the tumor necrosis factor (TNF) receptor super family 1A (TNFRSF1A) gene."

sparser
"The history of “autoinflammatory diseases” dates back to the identification of the genetic causes of the most prevalent monogenic autoinflammatory disease worldwide, the autosomal recessive disease, familial Mediterranean fever (FMF), in 1997, and the discovery of TNF receptor mutations in the autosomal dominant disorder, TNF receptor associated periodic syndrome (TRAPS) in 1999 ( xref - xref )."

sparser
"It is a rare autosomal dominant disease and strongly associated with heterozygous mutations in the tumor necrosis factor (TNF) receptor super family 1A (TNFRSF1A) gene."
| PMC

sparser
"This condition is a rare autosomal dominant disease that is strongly associated with heterozygous mutations in the tumor necrosis factor (TNF) receptor super family 1A (TNFRSF1A) gene."

sparser
"It is associated with autosomal dominant mutations in TNF receptor superfamily 1A gene localised to exons encoding the ectodomain of the p55 TNF receptor, TNF receptor-1 (TNFR1)."
RHO affects ATXN3
| 5
| 5

sparser
"This conclusion supports our recent report in which we showed that certain rhodopsin mutants associated with autosomal dominant retinitis pigmentosa also reconstitute into vesicles as monomers while retaining WT-like scramblase activity xref ."

sparser
"It is interesting to note that some of the rhodopsin mutations associated with autosomal dominant retinitis pigmentosa also result in defective protein trafficking."

sparser
"The most common Rhodopsin (Rh) mutation associated with autosomal dominant retinitis pigmentosa (ADRP) in North America is the substitution of proline 23 by histidine (Rh P23H )."

sparser
"Rhodopsin mutations are associated with the autosomal dominant form of retinitis pigmentosa."

sparser
"Mutant protein dimers have been observed also with mutant Rhodopsin (P23H) associated with autosomal dominant retinitis pigmentosa ( xref )."
MID1 affects ATXN7
| 5
| 3

sparser
"Furthermore, we show that this binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA, similar to what we have shown previously for HTT (Krauss et al., xref ), induces translation in a CAG repeat length-dependent manner in vitro and in cell lines."

sparser
"To test if the flanking regions influence the binding of MID1 to CAG repeat mRNAs, we tested the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNAs."

sparser
"Furthermore, we show that functionally, in line with what we have previously observed for HTT, the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA induces protein synthesis in a repeat length-dependent manner."
| 2

sparser
"In this study we show that MID1 can also bind to the CAG repeat region of ATXN2, ATXN3 , and ATXN7 in vitro , suggesting that binding of MID1 to CAG repeats is not dependent on the flanking regions."

sparser
"We show that ATXN2, ATXN3 , and ATXN7 bind to MID1 in a CAG repeat length-dependent manner."
MBNL1 affects ATXN3
| 2 3
MBNL1 binds ATXN3.
| 3
| 3

sparser
"Pentameric 5H-4 Potently Inhibits Formation of the SCA3 RNA-MBNL1 Interaction."

sparser
"As mentioned above, both MBNL1 and 5H-4 bind to RNA12 , which contains 12 copies of the 5′C A G/3′G A C motif that is present in toxic SCA3 repeats. xref Recently, the interaction of SCA3 RNAs with Muscleblind proteins has been implicated in the disease pathology of spinocerebellar ataxia type 3. xref Therefore, we sought to study the ability of 5H-4 to inhibit SCA3 RNA-MBNL1 interactions."

sparser
"Herein, we describe the design of cell permeable, modularly assembled ligands that inhibit the formation of the DM1 RNA- and SCA3 RNA-MBNL1 interactions with low nanomolar IC 50 's."
MBNL1 activates ATXN3.
| 2
MBNL1 activates ATXN3. 2 / 2
| 2

reach
"In experiments on polyQ-expanded ataxin-3 induced neurodegeneration in Drosophila, the authors found that the RNA binding protein MBNL1, a known player in neurodegeneration discussed below, enhances toxicity of a CAG-expanded SCA3 transgene."

reach
"Rather, our findings in FUS-linked ALS are consistent with previous work performed in a Drosophila model for spinocerebellar ataxia type 3, in which overexpression of MBNL1-enhanced ataxin-3 induced neurodegeneration ."
K63 affects ATXN3
| 3 2
ATXN3 binds K48- and K63. 3 / 3
| 3

reach
"In vitro, ataxin-3 binds K48- and K63 linked Ub chains at least four Ub long through its UIMs, preferentially cleaves chains longer than four Ub, and cleaves K63-linkages better than K48-ones [XREF_BIBR, XREF_BIBR]."

reach
"Interestingly, ataxin-3 binds to both K48- and K63 linked chains, but preferentially cleaves the latter chain type (Winborn et al., 2008)."

reach
"Mutant (expanded) ATXN3 still binds K48- and K63 linked polyUb chains, gets activated by ubiquitination, and retains DUB catalytic activity in vitro against K48 and K63 chains similarly to normal ATXN3, but expanded ATXN3 does appear to have an enhanced capacity to deubiquitinate K27- and K29 linked Ub chains."
| 2

sparser
"Ataxin-3 can also bind K48 and K63 linked polyubiquitin chains disregarding the length of the polyQ ( xref ; xref ; xref )."

sparser
"Ataxin-3 binds both K48- and K63-linked chains, however, it selectively hydrolyzes long K63-linked chains (albeit, extremely slowly) xref ."
IU1 affects ATXN3
| 5
IU1 activates ATXN3.
| 3
IU1 activates ATXN3. 3 / 3
| 3

reach
"IU1 (1-[1-(4-fluorophenyl) -2,5-dimethylpyrrol-3-yl]-2-pyrrolidin-1-ylethanone), an active-site-directed thiol protease inhibitor of USP14, was identified by a high-throughput screen and was shown to enhance degradation of tau, ataxin 3, and glial fibrillary acidic protein, independent of autophagy."

reach
"Conversely, a selective inhibitor of USP14, IU1, promoted degradation of overexpressed tau, TDP-43 and ataxin-3 [XREF_BIBR]."

reach
"In murine embryonic fibroblasts, IU1 treatment led to an increase in the degradation of the proteasome substrates Tau, TDP-43, and Atx3 that are critical in neurodegenerative diseases [XREF_BIBR]."
IU1 inhibits ATXN3.
| 2
IU1 inhibits ATXN3. 2 / 2
| 2

reach
"It was previously shown that IU1 was able to accelerate the degradation of tau, TDP43, ataxin 3, and glial fibrillary acidic protein7."

reach
"It was previously shown that IU1 was able to accelerate the degradation of tau, TDP43, ataxin 3, and glial fibrillary acidic protein XREF_BIBR."
EP300 affects ATXN3
2 | 1 2
2 | 1 2

No evidence text available

reach
"Atxn3 binds to p300 and inhibits its acetyltransferase activity."

sparser
"Therefore, Atxn3 may interact with p300 bound to the HSE transcription complex and inhibit p300 acetyltransferase activity, resulting in increased Hsf1 stress-induced activity."

sparser
"Atxn3 binds to p300 and inhibits its acetyltransferase activity (Li et al. xref )."

No evidence text available
E4B affects ATXN3
| 5
E4B inhibits ATXN3. 5 / 5
| 5

reach
"We suggest that loss of E4B, like loss of CHIP, would cause a buildup of expanded ataxin-3."

reach
"For example, E4B, a mammalian chain assembly factor (E3), is required to degrade the expanded form of ataxin 3 and is able to prevent aggregate formation of polyglutamines and even neurodegeneration i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Expression of E4B promoted degradation of a pathological form of ataxin-3."

reach
"E4B may have slightly enhanced the degradation of expanded ataxin-3 but this did not reach statistical significance; E4B overexpression had no effect on wild-type ataxin-3 (XREF_FIG, XREF_SUPPLEMENTARY)."

reach
"E4B has been verified to suppress the neurotoxicity of polyQ expanded ATXN3 in Drosophila models of SCA3 by promoting the ubiquitination and degradation of mutant ATXN3."
Caspase affects ATXN3
| 5
Caspase activates ATXN3. 5 / 5
| 5

reach
"CDK5 protects from caspase induced Ataxin-3 cleavage and neurodegeneration."

reach
"In line with previous research, there was no increased caspase mediated cleavage of expanded ataxin-3 compared to non expanded ataxin-3."

reach
"Whether caspase mediated ataxin-3 cleavage is indeed a major contributor to SCA3 pathogenesis remains to be determined through future in vivo experiments."

reach
"Finally, caspase mediated cleavage of expanded ataxin-3 resulted in increased ataxin-3 aggregation, suggesting a potential role for caspase mediated proteolysis in spinocerebellar ataxia type-3 pathogenesis."

reach
"A recent publication reported involvement of CDK5 in caspase mediated ataxin-3 cleavage, showing that RNAi of CDK5 in a Drosophila model for SCA 3 resulted in an enhanced SCA 3 toxicity [XREF_BIBR]."
CHDH affects ATXN3
| 5
| 5

sparser
"It is important to note that several of the autosomal dominant forms of CDH have been associated with reduced penetrance [ xref , xref , xref ]."

sparser
"De-novo and inherited autosomal dominant mutations in ZFPM2/FOG2 have been associated with isolated CDH, and CDH with CHD, and may account for up to 5% of the genetic causes of CDH [ xref – xref ]."

sparser
"If only multiplex pedigrees are enrolled, the proportion of probands with sporadic or autosomal dominant forms of CHD will be minimized."

sparser
"Heterozygous familial hypercholesterolemia (FH) is the most common genetic disorder associated with the development of premature CHD with an autosomal dominant mode of inheritance, and a prevalence of one case in 400 to 500 in the general population [ xref ]."

sparser
"Other rare autosomal dominant conditions associated with CDH."
ATXN7 affects MID1
| 5
| 3

sparser
"Furthermore, we show that this binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA, similar to what we have shown previously for HTT (Krauss et al., xref ), induces translation in a CAG repeat length-dependent manner in vitro and in cell lines."

sparser
"To test if the flanking regions influence the binding of MID1 to CAG repeat mRNAs, we tested the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNAs."

sparser
"Furthermore, we show that functionally, in line with what we have previously observed for HTT, the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA induces protein synthesis in a repeat length-dependent manner."
| 2

sparser
"In this study we show that MID1 can also bind to the CAG repeat region of ATXN2, ATXN3 , and ATXN7 in vitro , suggesting that binding of MID1 to CAG repeats is not dependent on the flanking regions."

sparser
"We show that ATXN2, ATXN3 , and ATXN7 bind to MID1 in a CAG repeat length-dependent manner."
ATXN3 affects spinocerebellar ataxia type 3
| 5
ATXN3 activates spinocerebellar ataxia type 3. 5 / 5
| 5

reach
"An obvious candidate gene in this region was Ataxin-3 (ATXN3), because expansion of a coding CAG repeat in ATXN3 causes spinocerebellar ataxia type 3 (SCA3 or Machado-Joseph disease [MJD, MIM 109150])[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"For instance, the ATXN3 gene usually contains 13-41 CAG repeats [XREF_BIBR]; more than 55 CAG repeats in the ATXN3 gene are pathogenic and can cause spinocerebellar ataxia type 3 (SCA3), which is a condition characterized by progressive problems with movement [XREF_BIBR]."

reach
"For example, spinocerebellar ataxia type 3 (SCA3), which is characterized by progressive dysfunction of the cerebellum and brain stem, is caused by polyglutamine (polyQ) expansion in the ataxin-3 gene."

reach
"A polyglutamine expansion mutation in ataxin-3 causes spinocerebellar ataxia type 3 (SCA3), thereby providing a further link between ubiquitin dependent protein quality control mechanisms and neurodegeneration XREF_BIBR, XREF_BIBR."

reach
"In addition, enhanced calpain activity aggravated pathogenesis of spinocerebellar ataxia type 3 (SCA3)."
| 5
| 5

reach
"d Spinocerebellar ataxia type 3 (SCA3): CK2 associates to and phosphorylates ataxin-3, thus promoting its nuclear localization and stabilization, and enhancing the formation of inclusions."

reach
"Coiled-Coil Structures of SCA3 polyQ Proteins Promote Their Nuclear Localization."

reach
"The product of this gene, ataxin-3, associates to and is phosphorylated by CK2 175 (Fig. 3d ), which induces its nuclear localization and stabilization, and enhances the formation of inclusions."

reach
"Mapping studies showed that two regions of Atx3, the Josephin domain and the C-terminus, regulated heat shock induced nuclear localization."

reach
"More evidence is needed to establish the physiological role of USP10 phosphorylation in tumorigenesis.In the case of ATX3, phosphorylation at Ser340 and Ser352 by CK2 enhances its nuclear localization, aggregation, and stability, processes that play a major role in the development of spinocerebellar ataxia-3."
ATXN3 affects UBQLN2
| 3 2
| 3 2

reach
"However, our assessment of coprecipitated ubiquitin in these studies did not reveal ubiquitinated HTT species and neither ruled in or out a ubiquitin dependent interaction.To examine whether ATXN3 sim[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"To examine whether ATXN3 similarly interacts with UBQLN2, we coexpressed flag-UBQLN2 or truncated UBQLN2 mutants with normal (28Q) or expanded (84Q) GFPATXN3 in HEK293 cells."

reach
"Given the difference in recruitment of UBQLN2 to disease protein inclusions in HD and SCA3, we investigated whether UBQLN2 interacts differentially with HTT and ATXN3."

sparser
"Consistent with this result, in a cell-based system mutant HTT interacts with UBQLN2 through the UBA domain while the SCA3 disease protein ATXN3, a deubiquitinating enzyme, does not interact with UBQLN2."

sparser
"To examine whether ATXN3 similarly interacts with UBQLN2, we coexpressed flag-UBQLN2 or truncated UBQLN2 mutants with normal (28Q) or expanded (84Q) GFPATXN3 in HEK293 cells."
ATXN3 affects Tubulin
| 2
| 2

sparser
"In fact, ataxin-3 is able to interact with tubulin through its JD domain (Figure xref ), with nM affinity (Mazzucchelli et al., xref ), which supports a role in cell structure."

sparser
"Previous reports showed that ATXN3 interacts with tubulin and microtubules ( xref ) and also with HDAC6 and dynein to promote aggresome transport to the microtubule organization center (MTOC) ( xref )."
ATXN3 affects TNF
| 5
| 5

sparser
"It is a rare autosomal dominant disease and strongly associated with heterozygous mutations in the tumor necrosis factor (TNF) receptor super family 1A (TNFRSF1A) gene."

sparser
"The history of “autoinflammatory diseases” dates back to the identification of the genetic causes of the most prevalent monogenic autoinflammatory disease worldwide, the autosomal recessive disease, familial Mediterranean fever (FMF), in 1997, and the discovery of TNF receptor mutations in the autosomal dominant disorder, TNF receptor associated periodic syndrome (TRAPS) in 1999 ( xref - xref )."

sparser
"It is a rare autosomal dominant disease and strongly associated with heterozygous mutations in the tumor necrosis factor (TNF) receptor super family 1A (TNFRSF1A) gene."
| PMC

sparser
"This condition is a rare autosomal dominant disease that is strongly associated with heterozygous mutations in the tumor necrosis factor (TNF) receptor super family 1A (TNFRSF1A) gene."

sparser
"It is associated with autosomal dominant mutations in TNF receptor superfamily 1A gene localised to exons encoding the ectodomain of the p55 TNF receptor, TNF receptor-1 (TNFR1)."
ATXN3 affects SOD2
| 3
ATXN3 increases the amount of SOD2. 3 / 5
| 3

reach
"Ataxin-3 has been shown to activate transcription factor forkhead box protein O4 (FOXO4) and induce the expression of manganese superoxide dismutase (SOD2), an antioxidant enzyme, under oxidative stress conditions; however, when ataxin-3 is expanded, this transcriptional activation effect is reduced and, in fact, SOD2 levels are decreased in SCA3 patients brain samples ."

reach
"Finally and consistent with a regulatory role of ATXN3 in SOD2 expression, knockdown of endogenous ATXN3 by RNA interference represses the expression of SOD2."

reach
"ATXN3 interacts with and stabilizes the forkhead box O (FOXO) transcription factor FOXO4, and upon oxidative stress they both translocate to the nucleus and activate manganese superoxide dismutase (SOD2) transcription which in turn protects cells from oxidative damage."
ATXN3 affects RHO
| 5
| 5

sparser
"This conclusion supports our recent report in which we showed that certain rhodopsin mutants associated with autosomal dominant retinitis pigmentosa also reconstitute into vesicles as monomers while retaining WT-like scramblase activity xref ."

sparser
"It is interesting to note that some of the rhodopsin mutations associated with autosomal dominant retinitis pigmentosa also result in defective protein trafficking."

sparser
"The most common Rhodopsin (Rh) mutation associated with autosomal dominant retinitis pigmentosa (ADRP) in North America is the substitution of proline 23 by histidine (Rh P23H )."

sparser
"Rhodopsin mutations are associated with the autosomal dominant form of retinitis pigmentosa."

sparser
"Mutant protein dimers have been observed also with mutant Rhodopsin (P23H) associated with autosomal dominant retinitis pigmentosa ( xref )."
ATXN3 affects KLF4
1 | 3 1
ATXN3 deubiquitinates KLF4.
1 | 2
ATXN3 deubiquitinates KLF4. 3 / 3
1 | 2

reach
"Additionally, ATXN3 was highly expressed in breast cancer and it promotes tumor tissue metastasis by deubiquitinating and stabilizing KLF4 [46]."

"We screened a library of 65 deubiquitinating enzymes and identified ATXN3 as a deubiquitinating enzyme of KLF4."

reach
"ATXN3 promotes breast cancer metastasis by deubiquitinating KLF4."
ATXN3 binds KLF4.
| 1 1
| 1 1

reach
"Subsequent immunoprecipitation assays confirmed that ATXN3 bound to KLF4, mediating the deubiquitination and stabilization of KLF4 protein levels."

sparser
"Subsequent immunoprecipitation assays confirmed that ATXN3 bound to KLF4, mediating the deubiquitination and stabilization of KLF4 protein levels."
ATXN3 affects K63
| 3 2
ATXN3 binds K48- and K63. 3 / 3
| 3

reach
"In vitro, ataxin-3 binds K48- and K63 linked Ub chains at least four Ub long through its UIMs, preferentially cleaves chains longer than four Ub, and cleaves K63-linkages better than K48-ones [XREF_BIBR, XREF_BIBR]."

reach
"Interestingly, ataxin-3 binds to both K48- and K63 linked chains, but preferentially cleaves the latter chain type (Winborn et al., 2008)."

reach
"Mutant (expanded) ATXN3 still binds K48- and K63 linked polyUb chains, gets activated by ubiquitination, and retains DUB catalytic activity in vitro against K48 and K63 chains similarly to normal ATXN3, but expanded ATXN3 does appear to have an enhanced capacity to deubiquitinate K27- and K29 linked Ub chains."
| 2

sparser
"Ataxin-3 can also bind K48 and K63 linked polyubiquitin chains disregarding the length of the polyQ ( xref ; xref ; xref )."

sparser
"Ataxin-3 binds both K48- and K63-linked chains, however, it selectively hydrolyzes long K63-linked chains (albeit, extremely slowly) xref ."
ATXN3 affects EP300
2 | 1 2
2 | 1 2

No evidence text available

reach
"Atxn3 binds to p300 and inhibits its acetyltransferase activity."

sparser
"Therefore, Atxn3 may interact with p300 bound to the HSE transcription complex and inhibit p300 acetyltransferase activity, resulting in increased Hsf1 stress-induced activity."

sparser
"Atxn3 binds to p300 and inhibits its acetyltransferase activity (Li et al. xref )."

No evidence text available
ATXN3 affects DNAJB1
| 5
ATXN3 increases the amount of DNAJB1. 5 / 5
| 5

reach
"When we conducted quantitative RT-PCR from fly heads that express polyQ78 in their eyes, we found that wild-type ataxin-3 increases the transcription levels of DnaJ-1."

reach
"When we conducted quantitative RT-PCR from fly heads that express polyQ78 in their eyes, we found that wild-type ataxin-3 increases the transcription levels of DnaJ-1 (XREF_FIG)."

reach
"These findings lead us to propose that ataxin-3, through its interaction with Rad23 and in a manner that depends on its deubiquitinase activity, leads to higher DnaJ-1 protein levels by increasing its transcription."

reach
"Altogether, our results propose a model whereby ataxin-3 increases DnaJ-1 levels in a manner that depends on the catalytic activity of this DUB and on its interaction with Rad23, and that DnaJ-1, acti[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Altogether, our results propose a model whereby ataxin-3 increases DnaJ-1 levels in a manner that depends on the catalytic activity of this DUB and on its interaction with Rad23, and that DnaJ-1, acting downstream of the ataxin-3-Rad23 interaction, suppresses degeneration by decreasing polyQ aggregates."
ATXN3 affects DNA repair
| 5
ATXN3 activates DNA repair.
| 3
| 3

reach
"As a DUB, ATXN3 stabilizes the ATR downstream targets Chk1 (Checkpoint Kinase 1) and p53 (tumor protein 53), both of which function to delay cell cycle progression and promote DNA repair."

reach
"Our studies described in the accompanying manuscript by Chatterjee et al suggest that PNKP is a native ATXN3 interacting protein, and that ATXN3 modulates PNKP activity and DNA repair (Chatterjee et al, Figs."

reach
"Clearly, loss of ATXN3 can impair multiple DNA repair processes."
ATXN3 inhibits DNA repair.
| 2
| 2

reach
"We propose that this mechanism ensures that ataxin-3 prevents the premature removal of DNA repair proteins only during the early phase of the DSB response and does not interfere with the subsequent timely displacement of DNA repair proteins by RNF4."

reach
"We propose that the immediate presence of ataxin-3 at DNA lesions prevents premature removal of MDC1 from DSBs and by this means reinforces initiation of DNA damage signaling and DNA repair (Fig XREF_FIG)."
ATXN3 affects CHDH
| 5
| 5

sparser
"It is important to note that several of the autosomal dominant forms of CDH have been associated with reduced penetrance [ xref , xref , xref ]."

sparser
"De-novo and inherited autosomal dominant mutations in ZFPM2/FOG2 have been associated with isolated CDH, and CDH with CHD, and may account for up to 5% of the genetic causes of CDH [ xref – xref ]."

sparser
"If only multiplex pedigrees are enrolled, the proportion of probands with sporadic or autosomal dominant forms of CHD will be minimized."

sparser
"Heterozygous familial hypercholesterolemia (FH) is the most common genetic disorder associated with the development of premature CHD with an autosomal dominant mode of inheritance, and a prevalence of one case in 400 to 500 in the general population [ xref ]."

sparser
"Other rare autosomal dominant conditions associated with CDH."
ATXN3 affects APC
| 5
| 5

sparser
"Pilomatrixomas are rare skin tumors that occur either sporadically or as a typical symptom of familial adenomatous polyposis (FAP, previously named Gardner syndrome), an autosomal dominant form of col[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Germline mutations of the APC gene are associated with an autosomal dominant precancerous condition, termed familial adenomatous polyposis (FAP)."

sparser
"Germline mutations (nonsense, frameshift) of APC are associated with familial adenomatous polyposis, an autosomal dominant syndrome, clinically characterized by young onset, hundreds of adenomatous polyps in the colon, and increased risk for extracolonic tumors."

sparser
"NSAIDs have been demonstrated to reduce polyp formation in patients with familial adenomatous polyposis, which is associated with autosomal dominant mutations in the APC gene and the activation of WNT–β-catenin signalling xref , xref ."

sparser
"Familial adenomatous polyposis (FAP) is an autosomal dominant precancerous condition which is associated with germline mutations of the APC gene."
ATXN2 affects MID1
| 5
| 3

sparser
"Furthermore, we show that this binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA, similar to what we have shown previously for HTT (Krauss et al., xref ), induces translation in a CAG repeat length-dependent manner in vitro and in cell lines."

sparser
"To test if the flanking regions influence the binding of MID1 to CAG repeat mRNAs, we tested the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNAs."

sparser
"Furthermore, we show that functionally, in line with what we have previously observed for HTT, the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA induces protein synthesis in a repeat length-dependent manner."
| 2

sparser
"In this study we show that MID1 can also bind to the CAG repeat region of ATXN2, ATXN3 , and ATXN7 in vitro , suggesting that binding of MID1 to CAG repeats is not dependent on the flanking regions."

sparser
"We show that ATXN2, ATXN3 , and ATXN7 bind to MID1 in a CAG repeat length-dependent manner."
APC affects ATXN3
| 5
| 5

sparser
"Pilomatrixomas are rare skin tumors that occur either sporadically or as a typical symptom of familial adenomatous polyposis (FAP, previously named Gardner syndrome), an autosomal dominant form of col[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Germline mutations of the APC gene are associated with an autosomal dominant precancerous condition, termed familial adenomatous polyposis (FAP)."

sparser
"Germline mutations (nonsense, frameshift) of APC are associated with familial adenomatous polyposis, an autosomal dominant syndrome, clinically characterized by young onset, hundreds of adenomatous polyps in the colon, and increased risk for extracolonic tumors."

sparser
"NSAIDs have been demonstrated to reduce polyp formation in patients with familial adenomatous polyposis, which is associated with autosomal dominant mutations in the APC gene and the activation of WNT–β-catenin signalling xref , xref ."

sparser
"Familial adenomatous polyposis (FAP) is an autosomal dominant precancerous condition which is associated with germline mutations of the APC gene."
Sirolimus affects ATXN3
| 4
Sirolimus decreases the amount of ATXN3. 4 / 4
| 4

reach
"Administration of a rapamycin analogue, CCI-779, to a SCA3 mouse model with an expanded ataxin-3 containing 70 glutamines, reduced soluble levels of expanded ataxin-3, decreased the number of aggregates in brains, and ameloriated motor dysfunction."

reach
"Consistent with the Drosophila data, the rapamycin analogue CCI-779 reduces both mutant huntingtin and ataxin-3 levels and ameliorates toxicity in mouse models of HD and spinocerebellar ataxia type 3, respectively XREF_BIBR, XREF_BIBR."

reach
"Consistent with the Drosophila data, the rapamycin analogue CCI-779 reduces both mutant huntingtin and ataxin-3 levels, thereby attenuating toxicity in mouse models of HD and spinocerebellar ataxia type 3, respectively."

reach
"CCI-779, a rapamycin ester (i.e., an analogue of rapamycin) reduces both mutant huntingtin and ataxin-3 levels, thereby attenuating toxicity in mouse models of HD and SCA3, respectively XREF_BIBR."
Polyubiquitin chains affects ATXN3
| 4
ATXN3 binds polyubiquitin chains. 4 / 4
| 4

reach
"Although its full biological role remains elusive, several studies have demonstrated that ataxin-3 binds and cleaves polyubiquitin chains, exhibiting a deubiquitinating like activity [XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR]."

reach
"Since ataxin-3 also associates with both the proteasome and polyubiquitin chains, it may recruit ubiquitylated substrates to the proteasome [XREF_BIBR]."

reach
"As a DUB, ATX3 is capable of binding polyubiquitin chains through its UIMs, preferring shortening polyubiquitin chains rather than complete disassembly."

reach
"Ataxin-3 preferentially binds polyubiquitin chains of four or more, the minimal ubiquitin chain length required for proteasomal targeting (Thrower et al., 2000)."
4 |
Hsa-miR-6851-5p decreases the amount of ATXN3. 4 / 4
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
4 |
Hsa-miR-6799-5p decreases the amount of ATXN3. 4 / 4
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
4 |
Hsa-miR-3689d decreases the amount of ATXN3. 4 / 4
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
Caa affects ATXN3
| 4
| 4

sparser
"HCHWA-D is an autosomal dominant form of CAA characterized by recurrent haemorrhages due to extensive Aβ deposition in cerebral blood vessel walls and diffuse amyloid plaques but absence of dense-core senile plaques or neurofibrillary tau pathology [ xref , xref , xref ] ( xref )."

sparser
"Hereditary autosomal dominant forms of CAA, in particular the most common form of Dutch-type hereditary CAA (D-CAA; also referred to in publications as hereditary cerebral hemorrhage with amyloidosis-Dutch type, HCHWA-D), xref offer the possibility of similar investigations of the early steps in the pathogenesis of CAA."

sparser
"Hereditary CysC amyloid angiopathy (HCCAA), also called hereditary cerebral hemorrhage with amyloidosis of Icelandic type, is an autosomal dominant form of CAA."

sparser
"Hereditary cerebral hemorrhage with amyloidosis, Icelandic type (HCHWA-I) (Arnason, xref ; Gudmundsson et al., xref ), also called hereditary cystatin C amyloid angiopathy (HCCAA; Olafsson et al., xref ), is an autosomal dominant form of CAA."
Ubiquitin affects RHEB
| 4
| 4

sparser
"Furthermore, the ATXN3 C14A mutant co-IPed more Ub-Rheb than wild-type ATXN3 ( xref ), suggesting that the binding of inactive ATXN3 to the Ub-Rheb may prevent its deubiquitination in the cells or during the purification of the ATXN3-Rheb complex."

sparser
"As expected, the four lysine residues of Rheb, which are responsible for the polyubiquitination of Rheb, are important for the binding of Ub-Rheb with ATXN3 ( xref )."

sparser
"In fact, higher levels of Ub-Rheb were co-IPed with ATXN3 when N-Ethylmaleimide, a deubiquitinase inhibitor, was included in the lysis and purification buffers ( xref ), supporting the idea that the Ub-Rheb that binds to ATXN3 was subjected to deubiquitination during the isolation processes."

sparser
"These observations support the idea that ATXN3 translocates to the lysosome and interacts with Ub-Rheb for its deubiquitination in a manner dependent on the inactive Rag heterodimer in response to amino acid starvation."
TRE affects ATXN3
| 4
ATXN3 binds TRE. 4 / 4
| 4

sparser
"The original stocking source (CHQ) clustered independently (mean q ‐value = 0.95) of derived individuals of JOSTRE stocking sources that grouped together ( q ‐value = 0.96)."

sparser
"While FRO lake still clustered with JOS, fish from MAU clustered independently ( q ‐value = 0.87) but with one individual showing a F 0 immigrant profile ( q ‐value > 0.95) from the JOSTRE stocking group and seven other individuals showing signs of introgression (0.10 <  q ‐value < 0.53) (Figure xref a,b)."

sparser
"Five Muskellunge (20%) from MSK displayed a q ‐value > 0.90, while 95% of remaining individuals showed introgression (0.13 <  q ‐value < 0.730) with the JOSTRE stocking source (Figure xref a, Figure xref )."

sparser
"The CHP site tended to form a separate cluster (mean q ‐value = 0.81) but with one F 0 immigrant from CHQ stocking source and five individuals (21%) showing admixture (F1 like profile) from the JOSTRE group (Figure xref a)."
SDS affects ATXN3
| 4
| 4

sparser
"Although nonexpanded ataxin-3 forms SDS-soluble fibrils, polyQ expansion induces a subsequent aggregation step in which these fibrils further assemble into large, highly stable, SDS-insoluble fibers ([MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"An interesting result in this study was the differing effects of SDS upon formation of SDS-insoluble ataxin-3 fibrils as opposed to the more commonly observed effects of SDS on SDS-soluble fibril formation."

sparser
"In the second stage of aggregation, only pathogenic length ataxin-3 forms SDS-insoluble fibrils which have a straighter morphology xref ."

sparser
"Addition of the polyQ-binding peptide QBP1 eliminated the formation of SDS-insoluble aggregates formed by ataxin-3(Q64) with 5 mM SDS."
SCA2 affects ATXN3
| 4
ATXN3 binds SCA2. 4 / 4
| 4

sparser
"SCA6 was the predominant genetic form, but also mutations associated with SCA2 and SCA3 were found in few patients [ xref ]."

sparser
"More specifically, we found that SCA3 associates with SCA2, while EA2 associates with SCA6 in the PCA space."

sparser
"Using Drosophila as a model for human disease, we now detail an interaction between the genes associated with SCA3 and SCA2."

sparser
"In this case, the fly has highlighted the significance of intriguing interactions between the genes that cause SCA2 and SCA3 diseases that can be supported by molecular and genetic findings."
Rad23 affects ATXN3
| 4
| 4

reach
"Ataxin-3 binds directly to at least two PQC related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; Fig. 1 A)."

reach
"Alternatively, the ataxin-3 and Rad23 complex could regulate DnaJ-1 levels by deubiquitinating transcription factors that control the levels of this gene."

reach
"Alternatively, the ataxin-3 and Rad23 complex could regulate DnaJ-1 levels by deubiquitinating transcription factors that control the levels of this gene.The transcription dependent model that we prop[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Ataxin-3 binds directly to at least two PQC related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; XREF_FIG)."
RHEB affects Ubiquitin
| 4
| 4

sparser
"Furthermore, the ATXN3 C14A mutant co-IPed more Ub-Rheb than wild-type ATXN3 ( xref ), suggesting that the binding of inactive ATXN3 to the Ub-Rheb may prevent its deubiquitination in the cells or during the purification of the ATXN3-Rheb complex."

sparser
"As expected, the four lysine residues of Rheb, which are responsible for the polyubiquitination of Rheb, are important for the binding of Ub-Rheb with ATXN3 ( xref )."

sparser
"In fact, higher levels of Ub-Rheb were co-IPed with ATXN3 when N-Ethylmaleimide, a deubiquitinase inhibitor, was included in the lysis and purification buffers ( xref ), supporting the idea that the Ub-Rheb that binds to ATXN3 was subjected to deubiquitination during the isolation processes."

sparser
"These observations support the idea that ATXN3 translocates to the lysosome and interacts with Ub-Rheb for its deubiquitination in a manner dependent on the inactive Rag heterodimer in response to amino acid starvation."
RET affects ATXN3
| 4
| 4

sparser
"Finally, germline RET mutations have been repeatedly associated with an autosomal dominant form of the Hirschsprung disease (HSCR) [10–13] , a congenital disorder of the enteric nervous system char[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Single-base pair missense mutations in exons 10, 11, 13, 14, 15, and 16 of the RET protooncogene are associated with the autosomal dominant multiple endocrine neoplasia type 2 (MEN2) syndromes: MEN2A, MEN2B, and familial medullary thyroid carcinoma."

sparser
"Mutations in RET are associated with autosomal dominant syndromes including MEN2A, MEN2B, familial MTC and are found in approximately 50% of sporadic cases [ xref ]."

sparser
"Most syndrome-related PCCs are associated with RET mutations, which cause the autosomal dominant MEN2 syndrome."
QBP1 affects ATXN3
| 4
QBP1 inhibits ATXN3. 4 / 4
| 4

reach
"It is conceivable that QBP1 might prevent ataxin-3 from transitioning into these states at later time points through the interaction described here.The importance of residues outside the polyQ domain in mediating the inhibition of aggregation reiterates the growing body of evidence for the importance of flanking domains in modulating and controlling amyloid aggregation."

reach
"To determine how QBP1 prevents amyloid fibril formation of ataxin-3 78Q, the ability of different variants, lacking one or more domains, to bind QBP1 was analysed using ESI-MS."

reach
"Ataxin-3 aggregation inhibition by QBP1 is maintained in volatile solvents."

reach
"Previous work has demonstrated that QBP1 inhibits the polyQ-dependent stage of ataxin-3 aggregation, while having no effect on polyQ-independent aggregation (Figure 2), but how the two species interact remained unknown."
PTEN affects ATXN3
| 4
| 4

sparser
"PTEN hamartoma tumour syndrome (PHTS) is a heterogeneous group of rare, autosomal dominant disorders associated with PTEN germline mutations."

sparser
"Human germline mutations in PTEN are associated with the rare autosomal dominant hamartomatous syndromes, Cowden Disease and Bannayan–Riley–Ruvalcaba syndrome, the former including an increased risk[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Germline mutation of PTEN is associated with rare autosomal dominant cancer syndromes such as Cowden disease and Lhermitte-Duclos disease, and leads to an increased susceptibility to cancer ( xref ; xref ; xref ; xref ; xref )."

sparser
"Considering the significance of PTEN as a tumor suppressor gene and that germline mutations in PTEN are associated with several autosomal dominant hamartoma syndromes [20] including Cowden's disease[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
NCL affects ATXN3
| 1 3
| 1 3

sparser
"Similar transcriptional alterations have been described in lymphocytes from patients with an autosomal dominant form of NCL caused by mutations in DNAJC5 , which encodes the synaptic protein cysteine-string protein alpha (CSPα) [ xref ]."

sparser
"In SCA3, this aberrant nucleolin- ATXN3 interaction decreases 45S pre-rRNA levels in cell and Drosophila models of SCA3 xref ."

reach
"Two peptides, P3 and P5, were found to be capable of interfering with NCL binding to MJD CAG78 RNA (XREF_FIG B)."

sparser
"While disruption of normal protein function by nonsense or missense mutations have been demonstrated to underlie the pathogenesis of autosomal recessive NCL, little has been known about the unique autosomal dominant form of NCL ( CLN4 ) [ xref , xref ]."
MYH9 affects ATXN3
| 4
| 4

sparser
"Furthermore, heterozygous mutations in the MYH9 have been associated with four autosomal dominant clinical syndromes characterized by neutrophil inclusion bodies, implying increased susceptibility for systemic inflammation in these individuals xref ."

sparser
"Mutations in MYH9 have been associated with four autosomal dominant clinical syndromes: May-Hegglin, Sebastian, Fechtner and Epstein xref ."

sparser
"MYH9 was an initially attractive candidate gene for kidney disease, because MYH9 mutations cause several rare diseases associated with autosomal dominant FSGS, including Fechtner syndrome and Epstein syndrome. xref , xref In further support of this association, GWAS conducted in African American and European American patients with FSGS showed that genetic variants in the region of chromosome 22 containing both MYH9 and APOL1 were strongly associated with increased risk of FSGS in African American individuals but not in European American individuals. xref However, when the DNA sequence of this region was examined in a larger, worldwide cohort, two APOL1 variants (named G1 and G2), rather than any variants in the MYH9 gene, accounted for the association with FSGS and hypertension-related kidney disease. xref These two APOL1 variants are common in African patients, but absent from European patients with FSGS."

sparser
"We further hypothesize that the MYH9 association with H-ESRD could explain the failure of intensive blood pressure reduction, including with the use of ACE inhibition, to slow nephropathy progression in hypertensive African Americans with chronic kidney disease. xref , xref , xref Prior to these reports, MYH9 was associated with rare autosomal dominant forms of macrothrombocytopenia, often with variable degrees of sensorineural deafness, cataracts, neutrophil inclusions, and glomerular disease. xref "
MFSD11 affects ATXN3
| 4
| 4

sparser
"Recently, exome sequencing in a large pedigree with an autosomal dominant form of familial ET proposed a rare mutation in the nuclear exporting signal region (NES; p."

sparser
"Because tremor is a common feature of mice with mutations in Scn8a , we explored the role of the human gene as a candidate gene for autosomal dominant (AD) form of ET."

sparser
"Therefore, in an attempt to identify a causative gene for ET, we performed WES and subsequent functional analyses in a family featuring an autosomal dominant form of early-onset ET."

sparser
"In this study, whole exome sequencing and subsequent approaches were performed in a family with an autosomal dominant form of early-onset ET."
Josephin domain affects ATXN3
| 3
Josephin domain activates ATXN3. 3 / 4
| 3

reach
"However, specific ubiquitination at lysine 117 in the Josephin domain activates ataxin-3 through an unknown mechanism."

reach
"When this is actually the case, as for example in the functional interaction between EGCG or related lower-weight compounds (EG, EGC) and the Josephin domain—which triggers the amyloid aggregation in expanded ATX3 variants [165,166,167]—well-established docking approaches are able to provide detailed information on the structural basis of the action of inhibitors [113]."

reach
"We show that changing the thermodynamic stability of the Josephin domain modulates ataxin-3 fibrillogenesis."
HFE affects ATXN3
| 4
| 4

sparser
"Autosomal dominant forms of hemochromatosis have also been described."

sparser
"Mutations in FPN1 (SLC40A1) lead to an autosomal dominant form of hemochromatosis (type 4), also known as ferroportin disease. xref Also, rare mutations in DMT1 (SLC11A2) are responsible for an absorptive defect leading to hypochromic microcytic anemia."

sparser
"More recently, mutations in the BMP6 gene affecting the processing of the precursor BMP6 pro-peptide to mature BMP6, were linked to an autosomal dominant form of mild hemochromatosis due to hepcidin deficiency ( xref – xref )."

sparser
"Mutations in FPN1 ( SLC40A1 ) lead to an autosomal dominant form of hemochromatosis (type 4), also known as ferroportin disease."
GJB2 affects ATXN3
| 4
| 4

sparser
"The autosomal dominant form, DFNA3, typically presents during childhood as a progressive, moderate to severe hearing impairment affecting mostly high frequencies."

sparser
"Specific autosomal dominant Cx26 mutations have been associated with syndromes of skin disease and hearing loss."

sparser
"There are a few specific mutations in GJB2 gene that are associated with the autosomal dominant syndromic forms of deafness, and typically present with skin abnormalities including keratitis-ichthyosis [ xref - xref ]."

sparser
"The majority of the over 100 deafness-causing mutations described in GJB2 causes ARNSHL, however 29 GJB2 mutations have been associated with autosomal dominant hearing loss (ADHL) ( xref )."
FGF23 affects ATXN3
| 4
| 2

sparser
"Specifically, we evaluated Cyp24 deficiency in Hyp mice, the murine homolog of X-linked dominant hypophosphatemic rickets, and transgenic mice that overexpress a mutant FGF23 (FGF23R176Q) that is associated with the autosomal dominant form of hypophosphatemic rickets."

sparser
"Several forms of hypophosphatemic rickets are known, including an X-linked form (MIM 307800) caused by phosphate regulating endopeptidase homolog, X-linked ( PHEX ) mutations (MIM 300550), an autosomal dominant form (MIM 193100) caused by fibroblast growth factor 23 ( FGF23 ) mutations (MIM 605380), an X-linked recessive form (MIM 300554) caused by chloride channel, voltage-sensitive 5 ( CLCN5 ) mutations (MIM 300008), and autosomal recessive forms caused by dentin matrix acidic phosphoprotein 1 ( DMP1 ) (MIM 600980), hypophosphatemic rickets, autosomal recessive 2 ( ARHR2 ) (MIM 613312) or ectonucleotide pyrophosphatase/phosphodiesterase 1 ( ENPP1 ) (MIM 173335) mutations. 'tiptoe walking' ( TTW ) mouse, which has a spontaneous nonsense mutation in ENPP1 is a good model for OPLL.[ xref ] Also, OPPL is a frequent complication in patients with endocrine disorders including hypoparathyroidism[ xref ] and acromegaly/gigantism.[ xref ] However, most cases of OPLL are idiopathic."
| 2

sparser
"The list of the diseases associated with OPLL includes hypophosphatemic rickets/osteomalacia, including an autosomal dominant form (MIM 193100) caused by FGF23 mutations, an X-linked dominant form (MIM 307800) caused by PHEX mutations, an X-linked recessive form (MIM 300554) caused by CLCN5 mutations, and autosomal recessive forms caused by DMP1 (MIM 600980) and ENPP1 (MIM 173335) mutations."

sparser
"Several forms of hypophosphatemic rickets are known, including an X-linked form (MIM 307800) caused by phosphate regulating endopeptidase homolog, X-linked ( PHEX ) mutations (MIM 300550), an autosomal dominant form (MIM 193100) caused by fibroblast growth factor 23 ( FGF23 ) mutations (MIM 605380), an X-linked recessive form (MIM 300554) caused by chloride channel, voltage-sensitive 5 ( CLCN5 ) mutations (MIM 300008), and autosomal recessive forms caused by dentin matrix acidic phosphoprotein 1 ( DMP1 ) (MIM 600980), hypophosphatemic rickets, autosomal recessive 2 ( ARHR2 ) (MIM 613312) or ectonucleotide pyrophosphatase/phosphodiesterase 1 ( ENPP1 ) (MIM 173335) mutations. 'tiptoe walking' ( TTW ) mouse, which has a spontaneous nonsense mutation in ENPP1 is a good model for OPLL.[ xref ] Also, OPPL is a frequent complication in patients with endocrine disorders including hypoparathyroidism[ xref ] and acromegaly/gigantism.[ xref ] However, most cases of OPLL are idiopathic."
EDA affects ATXN3
| 4
| 4

sparser
"The protein is a member of the tumor necrosis factor (TNF) receptor family, and is activated by its ligand EDA and uses EDARADD as an adaptor to build an intracellular NF-kB signal-transducing complex which is necessary for normal development of ectodermal organs both in humans and in mice [ xref , xref ], Autosomal dominant (AD) forms of HED have previously been linked to mutations in the ectodysplasin 1 anhidrotic receptor (EDAR) protein but mutations in the EDAR gene are also associated with recessive forms of ED [ xref , xref ]."

sparser
"Mutations in at least four genes (EDAR, EDARADD, WNT10A, TRAF6) have been reported to cause both autosomal recessive and autosomal dominant forms of HED."

sparser
"Germline heterozygous gain-of-function (GOF) mutations of NFKBIA , encoding IκBα, cause an autosomal dominant (AD) form of anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID)."

sparser
"I-kappaB alpha has specifically been associated with an autosomal dominant form of anhidrotic ectodermal dysplasia and with T cell immunodeficiency xref ."
CSA affects ATXN3
| 4
| 2

sparser
"In this study we show that MID1 can also bind to the CAG repeat region of ATXN2, ATXN3 , and ATXN7 in vitro , suggesting that binding of MID1 to CAG repeats is not dependent on the flanking regions."

sparser
"We show that ATXN2, ATXN3 , and ATXN7 bind to MID1 in a CAG repeat length-dependent manner."
| 2

sparser
"SCA3 is associated with CAG repeats that expand from the normal (12–37) to a pathogenic (61–84) range resulting in an extended polyGln tract in the C-terminus of the ataxin-3 (ATXN3) protein."

sparser
"A SNP 3′ to the ataxin-3 CAG repeat region is associated with the expansion and approximately seventy per cent of expanded CAG chromosomes carry the C variant ( xref )."
CRYGC affects ATXN3
| 4
| 4

sparser
"It has been shown that a mutation in the C-crystallin gene is associated with autosomal dominant congenital cataract in guinea-pigs and thus may be a candidate for human congenital cataract (Rao et a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"For example, mutation in the ζ-crystallin gene is associated with autosomal dominant hereditary cataract in a strain of guinea pigs ; normal mRNA for ζ-crystallin is absent in the lens of afflicted an[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Thus, to investigate the underlying etiology of hereditary cataracts, we used embryonic stem cell-based technologies to generate knock-in mice expressing proteins harboring either the αA-R49C or αB-R120G α-crystallin mutations, which are associated with human autosomal dominant hereditary cataracts [ xref , xref ]."

sparser
"Mutation in the ζ-crystallin gene is associated with an autosomal dominant hereditary cataract in strain 13/N guinea pigs [ 5 ]."
COL4A5 affects ATXN3
| 4
| 4

sparser
"We describe the pregnancy course of 8 pregnancies of three family members with the autosomal dominant (the rarest) form of Alport syndrome."

sparser
"The ocular and other clinical features of autosomal recessive Alport syndrome are identical to those seen in X-linked disease, while retinopathy and cataracts are the only ocular abnormalities described in the rare autosomal dominant form of Alport syndrome."

sparser
"The proportion of mutations in COL4A3 and COL4A4 was notably high, and the autosomal dominant forms of Alport syndrome appear more frequently than reported previously."

sparser
"Retinopathy and cataracts are the only ocular abnormalities described in the rare autosomal dominant form of Alport syndrome. xref Previous literature has reported variability in the incidence of ocular manifestations of Alport syndrome."
ATXN3 affects polyubiquitin chains
| 4
ATXN3 binds polyubiquitin chains. 4 / 4
| 4

reach
"Although its full biological role remains elusive, several studies have demonstrated that ataxin-3 binds and cleaves polyubiquitin chains, exhibiting a deubiquitinating like activity [XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR]."

reach
"Since ataxin-3 also associates with both the proteasome and polyubiquitin chains, it may recruit ubiquitylated substrates to the proteasome [XREF_BIBR]."

reach
"As a DUB, ATX3 is capable of binding polyubiquitin chains through its UIMs, preferring shortening polyubiquitin chains rather than complete disassembly."

reach
"Ataxin-3 preferentially binds polyubiquitin chains of four or more, the minimal ubiquitin chain length required for proteasomal targeting (Thrower et al., 2000)."

sparser
"Regulated association of atx3 with the ER membrane."

sparser
"The simplest explanation of these observations is that a small fraction of atx3 is transiently associated with the ER membrane via interactions with p97 and VIMP. atx3 may shuttle on and off p97 to promote PAD, a process that is linked to the ATPase cycle of p97 and to the subsequent delivery of substrates to the proteasome."

sparser
"We demonstrate that atx3 transiently associates with the ER membrane via p97 and the recently identified Derlin–VIMP complex, and its release from the membrane appears to be governed by both the p97 ATPase cycle and its own deubiquitinating activity."

sparser
"Together, these data suggest that a fraction of atx3 is associated with the ER membranes via interactions with components of the retrotranslocation machinery, including Derlin-1, VIMP, p97, and an ER-specific ubiquitin ligase."
ATXN3 affects caa
| 4
| 4

sparser
"HCHWA-D is an autosomal dominant form of CAA characterized by recurrent haemorrhages due to extensive Aβ deposition in cerebral blood vessel walls and diffuse amyloid plaques but absence of dense-core senile plaques or neurofibrillary tau pathology [ xref , xref , xref ] ( xref )."

sparser
"Hereditary autosomal dominant forms of CAA, in particular the most common form of Dutch-type hereditary CAA (D-CAA; also referred to in publications as hereditary cerebral hemorrhage with amyloidosis-Dutch type, HCHWA-D), xref offer the possibility of similar investigations of the early steps in the pathogenesis of CAA."

sparser
"Hereditary CysC amyloid angiopathy (HCCAA), also called hereditary cerebral hemorrhage with amyloidosis of Icelandic type, is an autosomal dominant form of CAA."

sparser
"Hereditary cerebral hemorrhage with amyloidosis, Icelandic type (HCHWA-I) (Arnason, xref ; Gudmundsson et al., xref ), also called hereditary cystatin C amyloid angiopathy (HCCAA; Olafsson et al., xref ), is an autosomal dominant form of CAA."
ATXN3 affects TRE
| 4
ATXN3 binds TRE. 4 / 4
| 4

sparser
"The original stocking source (CHQ) clustered independently (mean q ‐value = 0.95) of derived individuals of JOSTRE stocking sources that grouped together ( q ‐value = 0.96)."

sparser
"While FRO lake still clustered with JOS, fish from MAU clustered independently ( q ‐value = 0.87) but with one individual showing a F 0 immigrant profile ( q ‐value > 0.95) from the JOSTRE stocking group and seven other individuals showing signs of introgression (0.10 <  q ‐value < 0.53) (Figure xref a,b)."

sparser
"Five Muskellunge (20%) from MSK displayed a q ‐value > 0.90, while 95% of remaining individuals showed introgression (0.13 <  q ‐value < 0.730) with the JOSTRE stocking source (Figure xref a, Figure xref )."

sparser
"The CHP site tended to form a separate cluster (mean q ‐value = 0.81) but with one F 0 immigrant from CHQ stocking source and five individuals (21%) showing admixture (F1 like profile) from the JOSTRE group (Figure xref a)."
ATXN3 affects SDS
| 4
| 4

sparser
"Although nonexpanded ataxin-3 forms SDS-soluble fibrils, polyQ expansion induces a subsequent aggregation step in which these fibrils further assemble into large, highly stable, SDS-insoluble fibers ([MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"An interesting result in this study was the differing effects of SDS upon formation of SDS-insoluble ataxin-3 fibrils as opposed to the more commonly observed effects of SDS on SDS-soluble fibril formation."

sparser
"In the second stage of aggregation, only pathogenic length ataxin-3 forms SDS-insoluble fibrils which have a straighter morphology xref ."

sparser
"Addition of the polyQ-binding peptide QBP1 eliminated the formation of SDS-insoluble aggregates formed by ataxin-3(Q64) with 5 mM SDS."
ATXN3 affects SCA2
| 4
ATXN3 binds SCA2. 4 / 4
| 4

sparser
"SCA6 was the predominant genetic form, but also mutations associated with SCA2 and SCA3 were found in few patients [ xref ]."

sparser
"More specifically, we found that SCA3 associates with SCA2, while EA2 associates with SCA6 in the PCA space."

sparser
"Using Drosophila as a model for human disease, we now detail an interaction between the genes associated with SCA3 and SCA2."

sparser
"In this case, the fly has highlighted the significance of intriguing interactions between the genes that cause SCA2 and SCA3 diseases that can be supported by molecular and genetic findings."
ATXN3 affects Rad23
| 4
| 4

reach
"Ataxin-3 binds directly to at least two PQC related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; Fig. 1 A)."

reach
"Alternatively, the ataxin-3 and Rad23 complex could regulate DnaJ-1 levels by deubiquitinating transcription factors that control the levels of this gene."

reach
"Alternatively, the ataxin-3 and Rad23 complex could regulate DnaJ-1 levels by deubiquitinating transcription factors that control the levels of this gene.The transcription dependent model that we prop[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Ataxin-3 binds directly to at least two PQC related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; XREF_FIG)."
ATXN3 affects RHEB, and Ubiquitin
| 4
| 4

sparser
"Furthermore, the ATXN3 C14A mutant co-IPed more Ub-Rheb than wild-type ATXN3 ( xref ), suggesting that the binding of inactive ATXN3 to the Ub-Rheb may prevent its deubiquitination in the cells or during the purification of the ATXN3-Rheb complex."

sparser
"As expected, the four lysine residues of Rheb, which are responsible for the polyubiquitination of Rheb, are important for the binding of Ub-Rheb with ATXN3 ( xref )."

sparser
"In fact, higher levels of Ub-Rheb were co-IPed with ATXN3 when N-Ethylmaleimide, a deubiquitinase inhibitor, was included in the lysis and purification buffers ( xref ), supporting the idea that the Ub-Rheb that binds to ATXN3 was subjected to deubiquitination during the isolation processes."

sparser
"These observations support the idea that ATXN3 translocates to the lysosome and interacts with Ub-Rheb for its deubiquitination in a manner dependent on the inactive Rag heterodimer in response to amino acid starvation."
ATXN3 affects RET
| 4
| 4

sparser
"Finally, germline RET mutations have been repeatedly associated with an autosomal dominant form of the Hirschsprung disease (HSCR) [10–13] , a congenital disorder of the enteric nervous system char[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Single-base pair missense mutations in exons 10, 11, 13, 14, 15, and 16 of the RET protooncogene are associated with the autosomal dominant multiple endocrine neoplasia type 2 (MEN2) syndromes: MEN2A, MEN2B, and familial medullary thyroid carcinoma."

sparser
"Mutations in RET are associated with autosomal dominant syndromes including MEN2A, MEN2B, familial MTC and are found in approximately 50% of sporadic cases [ xref ]."

sparser
"Most syndrome-related PCCs are associated with RET mutations, which cause the autosomal dominant MEN2 syndrome."

reach
"The protein ataxin-3 (ATX3) triggers an amyloid-related neurodegenerative disease when its polyglutamine stretch is expanded beyond a critical threshold."

reach
"Spinocerebellar ataxia type 3/Machado-Joseph disease (SCA3/MJD) is a progressive autosomal dominant neurodegenerative disease caused by abnormal CAG repeats in the exon 10 of ATXN3."

reach
"The most common autosomal dominant ataxia worldwide, spinocerebellar ataxia type 3 (SCA3) is a fatal, progressive neurodegenerative disorder caused by a CAG trinucleotide repeat expansion in the ATXN3 gene."

reach
"Spinocerebellar ataxia type 3 (SCA3) is a neurodegenerative disease caused by a CAG repeat expansion in the ATXN3 gene."
ATXN3 affects NCL
| 1 3
| 1 3

sparser
"Similar transcriptional alterations have been described in lymphocytes from patients with an autosomal dominant form of NCL caused by mutations in DNAJC5 , which encodes the synaptic protein cysteine-string protein alpha (CSPα) [ xref ]."

sparser
"In SCA3, this aberrant nucleolin- ATXN3 interaction decreases 45S pre-rRNA levels in cell and Drosophila models of SCA3 xref ."

reach
"Two peptides, P3 and P5, were found to be capable of interfering with NCL binding to MJD CAG78 RNA (XREF_FIG B)."

sparser
"While disruption of normal protein function by nonsense or missense mutations have been demonstrated to underlie the pathogenesis of autosomal recessive NCL, little has been known about the unique autosomal dominant form of NCL ( CLN4 ) [ xref , xref ]."
ATXN3 affects MYH9
| 4
| 4

sparser
"Furthermore, heterozygous mutations in the MYH9 have been associated with four autosomal dominant clinical syndromes characterized by neutrophil inclusion bodies, implying increased susceptibility for systemic inflammation in these individuals xref ."

sparser
"Mutations in MYH9 have been associated with four autosomal dominant clinical syndromes: May-Hegglin, Sebastian, Fechtner and Epstein xref ."

sparser
"MYH9 was an initially attractive candidate gene for kidney disease, because MYH9 mutations cause several rare diseases associated with autosomal dominant FSGS, including Fechtner syndrome and Epstein syndrome. xref , xref In further support of this association, GWAS conducted in African American and European American patients with FSGS showed that genetic variants in the region of chromosome 22 containing both MYH9 and APOL1 were strongly associated with increased risk of FSGS in African American individuals but not in European American individuals. xref However, when the DNA sequence of this region was examined in a larger, worldwide cohort, two APOL1 variants (named G1 and G2), rather than any variants in the MYH9 gene, accounted for the association with FSGS and hypertension-related kidney disease. xref These two APOL1 variants are common in African patients, but absent from European patients with FSGS."

sparser
"We further hypothesize that the MYH9 association with H-ESRD could explain the failure of intensive blood pressure reduction, including with the use of ACE inhibition, to slow nephropathy progression in hypertensive African Americans with chronic kidney disease. xref , xref , xref Prior to these reports, MYH9 was associated with rare autosomal dominant forms of macrothrombocytopenia, often with variable degrees of sensorineural deafness, cataracts, neutrophil inclusions, and glomerular disease. xref "
ATXN3 affects MMP2
| 3
ATXN3 decreases the amount of MMP2. 3 / 4
| 3

reach
"On the one hand, ATXN3, HDAC3, and NCoR form a deacetylation complex to repress transcription of the MMP2 gene (Evert et al., 2006)."

reach
"Ataxin-3 is, for instance, able to repress matrix metalloproteinase-2 (MMP-2) transcription, and improved nuclear localisation of ataxin-3 through phosphorylation enhances this transcriptional repression [XREF_BIBR]."

reach
"Atxn3 UIMs were required to repress MMP2 transcription but Atxn3 catalytic DUB activity was not required."
ATXN3 affects MFSD11
| 4
| 4

sparser
"Recently, exome sequencing in a large pedigree with an autosomal dominant form of familial ET proposed a rare mutation in the nuclear exporting signal region (NES; p."

sparser
"Because tremor is a common feature of mice with mutations in Scn8a , we explored the role of the human gene as a candidate gene for autosomal dominant (AD) form of ET."

sparser
"Therefore, in an attempt to identify a causative gene for ET, we performed WES and subsequent functional analyses in a family featuring an autosomal dominant form of early-onset ET."

sparser
"In this study, whole exome sequencing and subsequent approaches were performed in a family with an autosomal dominant form of early-onset ET."
ATXN3 affects Histone
| 3 1
ATXN3 deacetylates Histone.
| 2
Modified ATXN3 leads to the deacetylation of Histone. 2 / 2
| 2

reach
"We next sought to identify the likely mechanism of increased histone acetylation induced by the loss of ATXN3."

reach
"Loss of ATXN3 induces acetylation of histones at Efna3 promoter region."
ATXN3 binds Histone.
| 1 1
| 1 1

sparser
"Interactions of ataxin-3 and other regulators of histone acetylation and transcription (p300, CREB-binding protein) have been detected ( xref )."

reach
"Although the exact cellular roles of ataxin 1 and 3 are not well understood yet, both seem to have nuclear functions: Ataxin1 is a chromatin binding factor and ataxin 3 binds to histones and regulates transcription."
ATXN3 affects HFE
| 4
| 4

sparser
"Autosomal dominant forms of hemochromatosis have also been described."

sparser
"Mutations in FPN1 (SLC40A1) lead to an autosomal dominant form of hemochromatosis (type 4), also known as ferroportin disease. xref Also, rare mutations in DMT1 (SLC11A2) are responsible for an absorptive defect leading to hypochromic microcytic anemia."

sparser
"More recently, mutations in the BMP6 gene affecting the processing of the precursor BMP6 pro-peptide to mature BMP6, were linked to an autosomal dominant form of mild hemochromatosis due to hepcidin deficiency ( xref – xref )."

sparser
"Mutations in FPN1 ( SLC40A1 ) lead to an autosomal dominant form of hemochromatosis (type 4), also known as ferroportin disease."
ATXN3 affects GJB2
| 4
| 4

sparser
"The autosomal dominant form, DFNA3, typically presents during childhood as a progressive, moderate to severe hearing impairment affecting mostly high frequencies."

sparser
"Specific autosomal dominant Cx26 mutations have been associated with syndromes of skin disease and hearing loss."

sparser
"There are a few specific mutations in GJB2 gene that are associated with the autosomal dominant syndromic forms of deafness, and typically present with skin abnormalities including keratitis-ichthyosis [ xref - xref ]."

sparser
"The majority of the over 100 deafness-causing mutations described in GJB2 causes ARNSHL, however 29 GJB2 mutations have been associated with autosomal dominant hearing loss (ADHL) ( xref )."
ATXN3 affects FGF23
| 4
| 2

sparser
"Specifically, we evaluated Cyp24 deficiency in Hyp mice, the murine homolog of X-linked dominant hypophosphatemic rickets, and transgenic mice that overexpress a mutant FGF23 (FGF23R176Q) that is associated with the autosomal dominant form of hypophosphatemic rickets."

sparser
"Several forms of hypophosphatemic rickets are known, including an X-linked form (MIM 307800) caused by phosphate regulating endopeptidase homolog, X-linked ( PHEX ) mutations (MIM 300550), an autosomal dominant form (MIM 193100) caused by fibroblast growth factor 23 ( FGF23 ) mutations (MIM 605380), an X-linked recessive form (MIM 300554) caused by chloride channel, voltage-sensitive 5 ( CLCN5 ) mutations (MIM 300008), and autosomal recessive forms caused by dentin matrix acidic phosphoprotein 1 ( DMP1 ) (MIM 600980), hypophosphatemic rickets, autosomal recessive 2 ( ARHR2 ) (MIM 613312) or ectonucleotide pyrophosphatase/phosphodiesterase 1 ( ENPP1 ) (MIM 173335) mutations. 'tiptoe walking' ( TTW ) mouse, which has a spontaneous nonsense mutation in ENPP1 is a good model for OPLL.[ xref ] Also, OPPL is a frequent complication in patients with endocrine disorders including hypoparathyroidism[ xref ] and acromegaly/gigantism.[ xref ] However, most cases of OPLL are idiopathic."
| 2

sparser
"The list of the diseases associated with OPLL includes hypophosphatemic rickets/osteomalacia, including an autosomal dominant form (MIM 193100) caused by FGF23 mutations, an X-linked dominant form (MIM 307800) caused by PHEX mutations, an X-linked recessive form (MIM 300554) caused by CLCN5 mutations, and autosomal recessive forms caused by DMP1 (MIM 600980) and ENPP1 (MIM 173335) mutations."

sparser
"Several forms of hypophosphatemic rickets are known, including an X-linked form (MIM 307800) caused by phosphate regulating endopeptidase homolog, X-linked ( PHEX ) mutations (MIM 300550), an autosomal dominant form (MIM 193100) caused by fibroblast growth factor 23 ( FGF23 ) mutations (MIM 605380), an X-linked recessive form (MIM 300554) caused by chloride channel, voltage-sensitive 5 ( CLCN5 ) mutations (MIM 300008), and autosomal recessive forms caused by dentin matrix acidic phosphoprotein 1 ( DMP1 ) (MIM 600980), hypophosphatemic rickets, autosomal recessive 2 ( ARHR2 ) (MIM 613312) or ectonucleotide pyrophosphatase/phosphodiesterase 1 ( ENPP1 ) (MIM 173335) mutations. 'tiptoe walking' ( TTW ) mouse, which has a spontaneous nonsense mutation in ENPP1 is a good model for OPLL.[ xref ] Also, OPPL is a frequent complication in patients with endocrine disorders including hypoparathyroidism[ xref ] and acromegaly/gigantism.[ xref ] However, most cases of OPLL are idiopathic."
ATXN3 affects ERAD
| 4
ATXN3 inhibits ERAD. 2 / 2
| 2

reach
"The observation that overexpression of wt atx3 moderately inhibits ERAD is unexpected, but is not without precedence."

reach
"Accordingly, inhibition of ERAD by ataxin-3 may allow more proteins to fold in the ER, and thus boosts the secretory capacity."
ATXN3-C14A inhibits ERAD. 2 / 2
| 2

reach
"The inhibition of ERAD by atx3 C14A can not be attributed to general perturbation of the ubiquitin proteasome system by the overexpressed defective DUB, because the proteasome dependent degradation of an N-end rule substrate Ub-R-GFP was not affected (XREF_FIG)."

reach
"To test whether atx3 C14A inhibits ERAD by interfering with the transfer of substrates from p97 to the proteasome, we determined the relative amount of ubiquitinated substrates bound to p97 versus those bound by the proteasome."
ATXN3 affects EDA
| 4
| 4

sparser
"The protein is a member of the tumor necrosis factor (TNF) receptor family, and is activated by its ligand EDA and uses EDARADD as an adaptor to build an intracellular NF-kB signal-transducing complex which is necessary for normal development of ectodermal organs both in humans and in mice [ xref , xref ], Autosomal dominant (AD) forms of HED have previously been linked to mutations in the ectodysplasin 1 anhidrotic receptor (EDAR) protein but mutations in the EDAR gene are also associated with recessive forms of ED [ xref , xref ]."

sparser
"Mutations in at least four genes (EDAR, EDARADD, WNT10A, TRAF6) have been reported to cause both autosomal recessive and autosomal dominant forms of HED."

sparser
"Germline heterozygous gain-of-function (GOF) mutations of NFKBIA , encoding IκBα, cause an autosomal dominant (AD) form of anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID)."

sparser
"I-kappaB alpha has specifically been associated with an autosomal dominant form of anhidrotic ectodermal dysplasia and with T cell immunodeficiency xref ."
ATXN3 affects CSA
| 4
| 2

sparser
"In this study we show that MID1 can also bind to the CAG repeat region of ATXN2, ATXN3 , and ATXN7 in vitro , suggesting that binding of MID1 to CAG repeats is not dependent on the flanking regions."

sparser
"We show that ATXN2, ATXN3 , and ATXN7 bind to MID1 in a CAG repeat length-dependent manner."
| 2

sparser
"SCA3 is associated with CAG repeats that expand from the normal (12–37) to a pathogenic (61–84) range resulting in an extended polyGln tract in the C-terminus of the ataxin-3 (ATXN3) protein."

sparser
"A SNP 3′ to the ataxin-3 CAG repeat region is associated with the expansion and approximately seventy per cent of expanded CAG chromosomes carry the C variant ( xref )."
ATXN3 affects CRYGC
| 4
| 4

sparser
"It has been shown that a mutation in the C-crystallin gene is associated with autosomal dominant congenital cataract in guinea-pigs and thus may be a candidate for human congenital cataract (Rao et a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"For example, mutation in the ζ-crystallin gene is associated with autosomal dominant hereditary cataract in a strain of guinea pigs ; normal mRNA for ζ-crystallin is absent in the lens of afflicted an[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Thus, to investigate the underlying etiology of hereditary cataracts, we used embryonic stem cell-based technologies to generate knock-in mice expressing proteins harboring either the αA-R49C or αB-R120G α-crystallin mutations, which are associated with human autosomal dominant hereditary cataracts [ xref , xref ]."

sparser
"Mutation in the ζ-crystallin gene is associated with an autosomal dominant hereditary cataract in strain 13/N guinea pigs [ 5 ]."
ATXN3 affects COL4A5
| 4
| 4

sparser
"We describe the pregnancy course of 8 pregnancies of three family members with the autosomal dominant (the rarest) form of Alport syndrome."

sparser
"The ocular and other clinical features of autosomal recessive Alport syndrome are identical to those seen in X-linked disease, while retinopathy and cataracts are the only ocular abnormalities described in the rare autosomal dominant form of Alport syndrome."

sparser
"The proportion of mutations in COL4A3 and COL4A4 was notably high, and the autosomal dominant forms of Alport syndrome appear more frequently than reported previously."

sparser
"Retinopathy and cataracts are the only ocular abnormalities described in the rare autosomal dominant form of Alport syndrome. xref Previous literature has reported variability in the incidence of ocular manifestations of Alport syndrome."
ATXN3 affects ATP2B3
| 4
| 4

sparser
"Asking whether aggregated proteins are asymmetrically inherited in polarized living tissue, the investigators studied human intestinal crypts of patient with spinocerebellar ataxia 3, a neurodegenerative disease associated with the accumulation of ataxin-3 polyglutamine-containing protein."

sparser
"These data suggest that a single locus at 14q32.1 is responsible for two forms of spinocerebellar ataxia, spinocerebellar ataxia type 3 and Machado-Joseph disease, and that this locus may account for approximately 11% of this group of dominantly inherited ataxias."

sparser
"Mutations in POLG1 gene have also been reported to be responsible for autosomal recessive and autosomal dominant forms of progressive external ophthalmoplegia, juvenile spinocerebellar ataxia-epilep[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"We focus on the nine neurodegenerative disorders caused by expansions of polyglutamine (polyQ) tracts, including spinal and bulbar muscular atrophy (SBMA), Huntington’s disease (HD), dentatorubropallidoluysian atrophy (DRPLA), and six autosomal dominant forms of spinocerebellar ataxia (SCA1, 2, 3, 6, 7, and 17)."
ATXN3 affects AS
| 4
| 4

sparser
"Various alterations in WFS1 gene including mutations and AS are associated with autosomal recessive Wolfram syndrome, autosomal dominant low frequency sensorineural hearing impairment (LFSNHI) DFNA6/14, some psychiatric diseases, and diabetes mellitus (Cryns et al., xref )."

sparser
"Some authors also recognize an autosomal dominant form of AS, caused by heterozygous COL4A3/A4 mutations and Alport-like ultrastructural histology xref , xref ."

sparser
"In scientific literature an autosomal dominant (AD) form of AS is also mentioned."

sparser
"There are very few reports in the literature, the vast majority of which from the pre-next generation sequencing (NGS) era where autosomal dominant form of AS reported, and only one mutation was found in either the COL4A3 or COL4A4 gene."
ATXN3 affects ANT1
| 4
ATXN3 binds ANT1. 4 / 4
| 4

sparser
"In humans, ANT1 mutations have been associated with autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions ( xref ) as well as autosomal recessive hypertrophic cardiomyopathy, myopathy, and lactic acidosis ( xref ). kdn encodes the Kreb cycle enzyme, citrate synthase."

sparser
"Autosomal dominant progressive external ophthalmoplegia (adPEO, MIM# 157640) is usually associated with mutations in the genes ANT1 , encoding the heart-muscle isoform of the adenine nucleotide trans[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"A familial mutation of ANT1 is associated with autosomal dominant progressive external ophthalmoplegia, characterized by large-scale mitochondrial DNA deletions [ xref ]."

sparser
"ANT1 has also been associated with a third condition, autosomal dominant facioscapulohumeral muscular dystrophy (FSHD), an adult onset disorder characterized by variable muscle weakness in the face, feet, shoulders, and hips."
ATXN3 affects AMFR
2 | 1
2 | 1

No evidence text available

No evidence text available

reach
"Here, we show that gp78 interacts with both SOD1 and ataxin-3."
ATXN1 affects ATXN3
| 4
ATXN1 binds ATXN3.
| 2
| 2

reach
"Interestingly, interaction of Atx1 and Atx3 with other proteins bearing polyQ revealed that the activity of Atx2 (SCA2) is critical for SCA3 and SCA1 pathogenesis [XREF_BIBR, XREF_BIBR]."

reach
"We also analyzed the possibility of genetic interactions between ATXN1 and ATXN2, ATXN2 and ATXN3, and ATXN2 and CACNA1A."
ATXN1 activates ATXN3.
| 2
ATXN1 activates ATXN3. 2 / 2
| 2

reach
"XREF_BIBR SCA1, SCA2, Machado-Joseph or SCA3, SCA6, SCA7, SCA12, SCA17, and dentatorubral-pallidoluysian atrophy (DRPLA) are caused by (CAG) n repeat expansions in the ATXN1, ATXN2, ATXN3, CACNA1A, ATXN7, PPP2R2B, TBP, and ATN1 genes, respectively, and all lead to the expansion of a polyglutamine tract in the corresponding proteins."

reach
"Ataxin gene variants in ATXN1, ATXN2, ATXN3, and ATXN7 cause SCA1, SCA2, SCA3, and SCA7, respectively."
ATP2B3 affects ATXN3
| 4
| 4

sparser
"Asking whether aggregated proteins are asymmetrically inherited in polarized living tissue, the investigators studied human intestinal crypts of patient with spinocerebellar ataxia 3, a neurodegenerative disease associated with the accumulation of ataxin-3 polyglutamine-containing protein."

sparser
"These data suggest that a single locus at 14q32.1 is responsible for two forms of spinocerebellar ataxia, spinocerebellar ataxia type 3 and Machado-Joseph disease, and that this locus may account for approximately 11% of this group of dominantly inherited ataxias."

sparser
"Mutations in POLG1 gene have also been reported to be responsible for autosomal recessive and autosomal dominant forms of progressive external ophthalmoplegia, juvenile spinocerebellar ataxia-epilep[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"We focus on the nine neurodegenerative disorders caused by expansions of polyglutamine (polyQ) tracts, including spinal and bulbar muscular atrophy (SBMA), Huntington’s disease (HD), dentatorubropallidoluysian atrophy (DRPLA), and six autosomal dominant forms of spinocerebellar ataxia (SCA1, 2, 3, 6, 7, and 17)."
AS affects ATXN3
| 4
| 4

sparser
"Various alterations in WFS1 gene including mutations and AS are associated with autosomal recessive Wolfram syndrome, autosomal dominant low frequency sensorineural hearing impairment (LFSNHI) DFNA6/14, some psychiatric diseases, and diabetes mellitus (Cryns et al., xref )."

sparser
"Some authors also recognize an autosomal dominant form of AS, caused by heterozygous COL4A3/A4 mutations and Alport-like ultrastructural histology xref , xref ."

sparser
"In scientific literature an autosomal dominant (AD) form of AS is also mentioned."

sparser
"There are very few reports in the literature, the vast majority of which from the pre-next generation sequencing (NGS) era where autosomal dominant form of AS reported, and only one mutation was found in either the COL4A3 or COL4A4 gene."
ANT1 affects ATXN3
| 4
ATXN3 binds ANT1. 4 / 4
| 4

sparser
"In humans, ANT1 mutations have been associated with autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions ( xref ) as well as autosomal recessive hypertrophic cardiomyopathy, myopathy, and lactic acidosis ( xref ). kdn encodes the Kreb cycle enzyme, citrate synthase."

sparser
"Autosomal dominant progressive external ophthalmoplegia (adPEO, MIM# 157640) is usually associated with mutations in the genes ANT1 , encoding the heart-muscle isoform of the adenine nucleotide trans[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"A familial mutation of ANT1 is associated with autosomal dominant progressive external ophthalmoplegia, characterized by large-scale mitochondrial DNA deletions [ xref ]."

sparser
"ANT1 has also been associated with a third condition, autosomal dominant facioscapulohumeral muscular dystrophy (FSHD), an adult onset disorder characterized by variable muscle weakness in the face, feet, shoulders, and hips."
TerC affects ATXN3
| 3
| 3

sparser
"Interestingly, the existence of oxidative stress in lymphocytes from patients with an autosomal dominant form of DC with mutations in TERC has been recently reported xref ."

sparser
"This theory was supported by the description of an autosomal dominant form of DC, caused by mutations in TERC [ xref ]."

sparser
"Further analysis indicated that transcriptional silencing owing to a 3′ deletion in the TERC locus leads to the autosomal dominant form of dyskeratosis congenita by diminishing TERC transcription."
| 3
| 3

reach
"This analysis identified only 16 genes (XREF_TABLE) whose expression was decreased in transgenic ataxin-3 mice and increased in transgenic mice treated with temsirolimus."

reach
"Of the first three genes we looked at, we confirmed a decrease in expression of Usp15 and Grik2 between wild-type and ataxin-3 transgenic mice, which were reversed in mice treated with temsirolimus (XREF_FIG B)."

reach
"A drug that increases autophagy, temsirolimus, decreased ataxin-3 both in soluble and aggregate forms and reduced pathology in a SCA3-transgenic line."
P97 affects ATXN3
| 3
ATXN3 binds p97. 3 / 3
| 3

sparser
"Because Atx3 binds P97 through a VBM motif xref , we applied isoform I of Atx3 (Atx3-I) as a molecular model to explore the relationship between sequestration and specific interaction ( xref )."

sparser
"Interestingly, mutation in the VBM motif of Atx3 100Q (Atx3 100Q /VBM*, 282 RKRR to HNHH), which disrupts the specific interaction between Atx3 and P97, significantly abolished the sequestration of P97 into aggregates; even the amount of P97 sequestered by the mutant was less than that by Atx3 22Q under the condition of similar amounts of the Atx3 aggregates ( xref )."

sparser
"Although it cannot be excluded that the lower amount of the aggregates may have some correlation to the decreased sequestration, we think that the major contribution to the decreased ability of the VBM mutant to sequester P97 is from disruption of the specific interaction between Atx3 100Q and P97."
Hsa-miR-921 affects ATXN3
3 |
Hsa-miR-921 decreases the amount of ATXN3. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
3 |
Hsa-miR-6134 decreases the amount of ATXN3. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
3 |
Hsa-miR-6073 decreases the amount of ATXN3. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
3 |
Hsa-miR-4537 decreases the amount of ATXN3. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
3 |
Hsa-miR-382-5p decreases the amount of ATXN3. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
Hsa-miR-3190-3p affects ATXN3
3 |
Hsa-miR-3190-3p decreases the amount of ATXN3. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
3 |
Hsa-miR-3174 decreases the amount of ATXN3. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
3 |
Hsa-miR-142-3p decreases the amount of ATXN3. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
3 |
Hsa-miR-1247-3p decreases the amount of ATXN3. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
VHL affects ATXN3
| 3
| 3

sparser
"VHL disease, an autosomal dominant disorder, is associated with structural abnormalities on chromosome 3p and is characterized by a predisposition to a variety of neoplasms, including RCC."

sparser
"The most common subtype, clear cell RCC, is associated with autosomal dominant mutation of the Von Hippel-Lindau gene (VHL).[ xref ] Smoking and obesity are other known risk factors.[ xref ] Papillary RCC is the second most common subtype."

sparser
"Mutations in VHL are associated with lung cancer, sporadic clear cell renal carcinomas and an autosomal dominant familial cancer known as von Hippel-Lindau disease ([ xref - xref ] and see [ xref ])."
VCP affects RAD23 family
| 3
| 3

sparser
"Ataxin-3 interacts with ubiquitinated substrates, p97, Rad23, and the proteasome, and the expanded-polyglutamine-containing ataxin-3 is defective in the substrate degradation [74] ."

sparser
"Ataxin-3 binds directly to at least two PQC-related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; xref )."

sparser
"Ataxin-3 binds directly to at least two PQC-related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; Fig. 1 A)."
UMOD affects ATXN3
| 3
| 3

sparser
"Mutations in the UMOD gene are associated with medullary cystic kidney disease and autosomal dominant tubulointerstitial kidney disease [ xref , xref ]."

sparser
"The role of UMOD in normal kidney function is incompletely understood, however, mutations of the UMOD gene are associated with autosomal dominant tubulointerstitial kidney disease (ADTKD- UMOD ) which as previously been known as familial juvenile hyperuricaemic nephropathy (FJHN) and medullary cystic kidney disease (MCKD2), also known as UMOD-associated kidney diseases [ xref , xref , xref , xref , xref ]."

sparser
"It should be noted, however, that irrespective of genetic phenotypes, in both CHD populations and cohorts with established Autosomal Dominant Tubulointerstitial Kidney Disease (ADTKD-UMOD), serum uromodulin concentrations were independently associated with hard outcomes in the former [ xref ], or were lower in all affected patients with a novel missense mutation in the UMOD gene (457T>G; Cys153Gly), relative to all patients of the reference eGFR-matched CKD groups, and healthy, unaffected family members [ xref ]."
UBQLN1 affects ATXN3
1 1 | 1
1 1 | 1

No evidence text available

No evidence text available

reach
"Here, we show that PLIC-1 also binds to the UIM proteins ataxin 3 -- a deubiquitinating enzyme -- HSJ1a -- a co-chaperone -- and EPS15 (epidermal growth factor substrate 15) -- an endocytic protein."
TRIP11 affects ATXN3
| 3
| 3

sparser
"B4GALNT1 , G2E3-SCFD1 , and TRIP11-ATXN3 SNPs did not reach genome-wide significance, but the genes achieved significance in a gene-based analysis that combines all SNP association signals within a gene."

sparser
"We have identified one additional ALS locus, ACSL5-ZDHHC6 , and three additional putative loci, B4GALNT1 , G2E3-SCFD1 , and TRIP11-ATXN3 , with potential functional relevance for ALS."

sparser
"Moreover, B4GALNT1, G2E3-SCFD1, and TRIP11-ATXN3 are identified using a gene-based analysis."
STK11 affects ATXN3
| 3
| 3

sparser
"However, oligonucleotide aCGH demonstrated a de novo 1.1-Mb deletion of 19p13.3 including STK11 , which is associated with autosomal dominant Peutz-Jeghers syndrome (PJS)( xref )."

sparser
"Liver kinase B1 (LKB1, STK11) is key regulatory protein of cellular metabolism that was originally identified in patients with Peutz-Jeghers syndrome (PJS), an autosomal dominant disease associated with LKB1 germline alterations xref ."

sparser
"Loss-of-function germline mutations of LKB1 are associated with Peutz–Jeghers syndrome (PJS), an autosomal dominant disorder."
SIGE affects ATXN3
| 3
| 3

sparser
"The autosomal dominant hyper IgE syndrome is associated with a missense or in-frame deletions in the SH2 and DNA-binding domains in the STAT3 gene (Signal Transducer and Activator of Transcription 3) [ xref ]."

sparser
"Indeed, patients with the autosomal dominant form of hyper IgE syndrome (HIES) have severely reduced numbers of IL-17 producing circulating T cells due to dominant-negative mutations of the signal transducer and activator of transcription 3 ( STAT3 ), and often suffer from CMC [ xref – xref ]."

sparser
"DOCK8 deficiency gives rise to an autosomal recessive form of the hyper IgE (AR-HIES) syndrome that overlaps in its features with autosomal dominant hyper IgE (AD-HIES) syndrome caused by loss of function mutations in STAT3 ( xref – xref )."
SCN5A affects ATXN3
| 3
| 3

sparser
"Brugada syndrome is a life-threatening, inherited arrhythmia disorder associated with autosomal dominant mutations in SCN5A [ xref - xref ], the gene encoding the human cardiac Na + channel α subunit (Nav1.5) [ xref ], which contains four homologous domains, each composed of six membrane-spanning segments, linked by cytoplasmic linkers."

sparser
"Brugada syndrome (BrS) is a life-threatening, inherited arrhythmogenic syndrome associated with autosomal dominant mutations in SCN5A , the gene encoding the cardiac Na + channel alpha subunit (Na v 1.5)."

sparser
"Brugada syndrome is a life-threatening, inherited arrhythmia disorder associated with autosomal dominant mutations in SCN5A, the gene encoding the human cardiac Na + channel α subunit (Nav1.5)."
SCD affects ATXN3
| 3
| 3

sparser
"Autosomal dominant forms of SCD have also been reported, but to date no genetic etiology has been described for these."

sparser
"This is the first identification of the genetic cause of an autosomal dominant form of SCD, and also demonstrates the potential of exome sequencing to identify genetic causes of dominant diseases even in small families with few affected individuals."

sparser
"Recently, an autosomal dominant form of SCD has been attributed to TBX6 mutation [ xref ]."
RAD23 family affects VCP
| 3
| 3

sparser
"Ataxin-3 interacts with ubiquitinated substrates, p97, Rad23, and the proteasome, and the expanded-polyglutamine-containing ataxin-3 is defective in the substrate degradation [74] ."

sparser
"Ataxin-3 binds directly to at least two PQC-related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; xref )."

sparser
"Ataxin-3 binds directly to at least two PQC-related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; Fig. 1 A)."
Protease affects ATXN3
| 3
| 3

reach
"It was discovered that inhibition of Ca 2+ -dependent protease calpain suppressed aggregation of polyglutamine expanded Atxn3 in transfected cells."

reach
"It was discovered that inhibition of Ca 2+ -dependent protease calpain suppressed aggregation of polyglutamine expanded Atxn3 in transfected cells [XREF_BIBR]."

reach
"It was discovered that inhibition of Ca 2+ -dependent protease calpain suppressed aggregation of polyglutamine expanded Atxn3 in transfected cells [XREF_BIBR]."
PTCH1 affects ATXN3
| 3
| 3

sparser
"Importantly, TSPAN8 overexpression enhanced the binding of ATXN3 to PTCH1 in MCF-7 cells, whereas TSPAN8 depletion reduced their interaction (Fig.  xref )."

sparser
"These results suggest that TSPAN8 facilitates the binding of ATXN3 to PTCH1 to reduce the degradation of PTCH1."

sparser
"These results indicate that TSPAN8 stabilizes PTCH1 expression by promoting the binding of ATXN3 to PTCH1, leading to deubiquitylation of PTCH1."
PSMD4 affects ATXN3
| 3
| 3

sparser
"This study describes a previously unreported autosomal dominant form of heritable AF, with early age of onset, associated conduction system disease and atrial myopathy."

sparser
"In pull-down assays, ubiquitination of CHIP enhanced its interaction with both S5a and ataxin-3 ( xref )."

sparser
"The identification of a single genetic variant that associates with the autosomal dominant AF subtype, in more than one unrelated family, would represent a significant breakthrough in understanding the genetics and molecular mechanisms for the manifestation of AF."
PRSS1 affects ATXN3
| 3
| 3

sparser
"Germline mutations in the PRSS1 gene, which encodes cationic trypsinogen, has been associated with an autosomal dominant form of hereditary pancreatitis."

sparser
"The most common cause of hereditary pancreatitis is an autosomal dominant form caused by mutations in the PRSS1 gene which account for up to 80% of cases [ xref ]."

sparser
"Heterozygous PRSS1 mutations are often associated with autosomal dominant hereditary disease, indicating that these are strong, essentially causative genetic changes [ xref , xref and references therein]."
PPK affects ATXN3
| 3
ATXN3 binds PPK. 3 / 3
| 3

sparser
"Taken together, these results suggest that CDH12 gene is a potential candidate gene for an atypical presentation of an autosomal dominant form of transgrediens and progrediens PPK."

sparser
"In this study, we identified five families of Pakistani origin with an autosomal dominant form of PPK."

sparser
"We investigated at the clinical and genetic level a consanguineous Tunisian family presenting an autosomal dominant atypical form of transgrediens and progrediens PPK to better characterize this ultrarare disease and to identify its molecular etiology."
PABPN1 affects ATXN3
| 3
| 3

sparser
"Initial symptoms in terms of dysphagia appeared at the age of 70, much later than the usual disease onset of the autosomal dominant form of OPMD, which usually becomes symptomatic at the age between 40 and 60 years."

sparser
"A hallmark of the autosomal dominant form of OPMD is the presence of nuclear aggregates in muscle tissue [ xref ] similar to other polyalanine and polyglutamine diseases [ xref – xref ]."

sparser
"Patient 2 (G.F.) was a 63 year old man with a clinically diagnosed autosomal dominant form of OPMD."
NOTCH3 affects ATXN3
| 3
| 3

sparser
"Moreover, Notch3 mutation is associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) [ xref , xref ]."

sparser
"NOTCH3 has been associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), a dementia disorder which clinical phenotype overlaps with EOAD."

sparser
"Mutations in human NOTCH3 are associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), a disorder that causes stroke and dementia and is accompanied by the degeneration of vascular smooth muscle cells [ xref ]; adult Notch3 mutant mice display a defect in the maturation of arterial smooth muscle cells [ xref ]."
NCOR1 affects HDAC3
| 3
| 3

sparser
"Both normal (Q23) and expanded (Q70) human full-length Ataxin-3 physiologically interacted with HDAC3 and NCoR in a SCA3 rat cell line and human pons tissue."

sparser
"A direct interaction of ATXN3 with HDAC3 and NCoR has previously been established in SCA3 cell lines and human brain extracts yet with only normal (i.e. not expanded) ATXN3 being associated with increased deacetylase activity and repression of gene expression [ xref ]."

sparser
"Further, it was shown that both normal and expanded ataxin 3 physiologically interact with HDAC3 and NCoR in a SCA3 cell model and in human pons tissue; however, normal ataxin 3-containing protein complexes showed increased histone deacetylase activity, whereas polyglutamine-expanded ataxin 3-containing complexes had reduced deacetylase activity in target chromatin regions [ xref ]."
MYC affects ATXN3
| 3
| 3

sparser
"Reciprocally, Myc-ATXN3 co-IPed both ubiquitin-free Flag-Rheb and Ub-Flag-Rheb ( xref )."

sparser
"Notably, the expression of Myc-ATXN3 but not its enzyme-inactive mutant (C14A) reduced levels of Ub-Rheb in the lysate ( xref and xref )."

sparser
"Flag-Rheb but not Flag-Rap2a, which is also expressed on the lysosome ( xref ), specifically co-IPed Myc-ATXN3 ( xref )."
MSC affects ATXN3
| 2 1
MSC inhibits ATXN3. 3 / 3
| 2 1

reach
"In the present study, we aim to investigate whether MSC derived exosomes can slow down the disease progression in a transgenic mouse model of MJD."

reach
"This observation is consistent with a previous report that MSC did not directly inhibit ATXN3 in a mouse model of MJD [XREF_BIBR]."

sparser
"This observation is consistent with a previous report that MSC did not directly inhibit ATXN3 in a mouse model of MJD [ xref ]."
MAP1LC3C affects GABARAP
| 3
| 3

sparser
"Ataxin-3 preferentially binds GABARAP and LC3C with either of its two recently identified LIR motifs."

sparser
"Moreover, Ataxin-3 interacts with the autophagosomal membrane proteins LC3C and GABARAP and transiently localized to early autophagosomes."

sparser
"ATXN3 interacts directly with GABARAP and LC3C."
KAT2B affects ATXN3
2 | 1
2 | 1

No evidence text available

No evidence text available

reach
"showed that Atxn3 interacted with three major histone acetyltransferases, CBP, p300, and p300 and CBP associated factor and inhibited their acetyltransferase activity resulting in repression of model gene promoters."
K63 affects K48-
| 3
ATXN3 binds K48- and K63. 3 / 3
| 3

reach
"In vitro, ataxin-3 binds K48- and K63 linked Ub chains at least four Ub long through its UIMs, preferentially cleaves chains longer than four Ub, and cleaves K63-linkages better than K48-ones [XREF_BIBR, XREF_BIBR]."

reach
"Interestingly, ataxin-3 binds to both K48- and K63 linked chains, but preferentially cleaves the latter chain type (Winborn et al., 2008)."

reach
"Mutant (expanded) ATXN3 still binds K48- and K63 linked polyUb chains, gets activated by ubiquitination, and retains DUB catalytic activity in vitro against K48 and K63 chains similarly to normal ATXN3, but expanded ATXN3 does appear to have an enhanced capacity to deubiquitinate K27- and K29 linked Ub chains."
ITPR1 affects HTT
| 3
ATXN2 binds HTT, ITPR1, and ATXN3. 3 / 3
| 3

sparser
"Importantly, mutant HTT, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of IP3R1 in rat neurons and increase its sensitivity to InsP3 [ xref , xref ]."

sparser
"My laboratory discovered that mutant Huntingtin, ataxin-2 and ataxin-3 proteins specifically binds to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP 3 R1), an intracellular Ca 2+ release channel."

sparser
"Our laboratory discovered that mutant huntingtin, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP(3)R1), an intracellular Ca(2+) release channel."
ITCH affects ATXN3
2 | 1
2 | 1

No evidence text available

No evidence text available

sparser
"We observed only the interaction of ITCH with expanded-polyglutamine ataxin-3(84Q) proteins ( xref )."
HTT affects ITPR1
| 3
ATXN2 binds HTT, ITPR1, and ATXN3. 3 / 3
| 3

sparser
"Importantly, mutant HTT, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of IP3R1 in rat neurons and increase its sensitivity to InsP3 [ xref , xref ]."

sparser
"My laboratory discovered that mutant Huntingtin, ataxin-2 and ataxin-3 proteins specifically binds to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP 3 R1), an intracellular Ca 2+ release channel."

sparser
"Our laboratory discovered that mutant huntingtin, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP(3)R1), an intracellular Ca(2+) release channel."
HTRA1 affects ATXN3
| 3
| 3

sparser
"Recently, other heterogeneous autosomal dominant disorders have been associated with heterozygous HTRA1 gene mutations [ xref ]."

sparser
"For instance, an état criblée of the basal ganglia may be more prevalent in HTRA1 autosomal dominant related forms [ xref ]."

sparser
"The few studies on the relative frequency of these disorders indicate that the most frequent (or rather the least rare), accounting for more than half of patients, is CADASIL, due to mutations of the NOTCH3 gene, followed by COL4A1/A2-related disease, autosomal dominant forms of HTRA1-related disease and leucoencephalopathies with calcifications and cysts."
HSPA8 affects ATXN3
| 3
| 3

sparser
"The model above focuses on the role of the UIMs facilitating an interaction between ataxin-3 and Hsc70-4, because pathogenic ataxin-3 with mutated UIMs does not co-IP Hsc70-4."

sparser
"UIMs mediate ataxin-3 interaction with Hsc70-4."

sparser
"It may also be that under normal circumstances ataxin-3 forms a functional complex with Hsc70-4, collaborating in protein quality control."
HSP104 affects ATXN3
| 3
HSP104 inhibits ATXN3. 3 / 3
| 3

reach
"Importantly, in staging experiments, Hsp104 suppressed toxicity of a C-terminal MJD fragment when expressed after the onset of PolyQ induced degeneration, whereas Hsp70 was ineffective."

reach
"XREF_BIBR Notably, Hsp104 suppressed toxicity of a C-terminal ataxin-3 fragment when expressed even after the onset of polyglutamine induced degeneration."

reach
"Hsp104 suppressed toxicity of MJD variants lacking a portion of the N-terminal deubiquitylase domain and full-length MJD variants unable to engage polyubiquitin, indicating that MJD-ubiquitin interactions hinder protective Hsp104 modalities."
HDAC3 affects NCOR1
| 3
| 3

sparser
"Both normal (Q23) and expanded (Q70) human full-length Ataxin-3 physiologically interacted with HDAC3 and NCoR in a SCA3 rat cell line and human pons tissue."

sparser
"A direct interaction of ATXN3 with HDAC3 and NCoR has previously been established in SCA3 cell lines and human brain extracts yet with only normal (i.e. not expanded) ATXN3 being associated with increased deacetylase activity and repression of gene expression [ xref ]."

sparser
"Further, it was shown that both normal and expanded ataxin 3 physiologically interact with HDAC3 and NCoR in a SCA3 cell model and in human pons tissue; however, normal ataxin 3-containing protein complexes showed increased histone deacetylase activity, whereas polyglutamine-expanded ataxin 3-containing complexes had reduced deacetylase activity in target chromatin regions [ xref ]."
HCRT affects ATXN3
| 3
| 3

sparser
"A robust increase (64–94%) in the number of histamine neurons in the TMN has been reported by two independent groups ( xref ). xref , xref However, we did not observe this increase of histamine neurons in a series of narcoleptic animal models we tested (hypocretin 2R mutant dogs, Hcrt knock-out mice, ataxin-3-hypocretin mice, and doxycycline-controlled diphtheria toxin A–hypocretin mice). xref Of these animal models, the postnatal hypocretin cell loss and adult-onset symptoms seen in the doxycycline-controlled diphtheria toxin A–hypocretin mouse resembles the clinical characteristics human narcolepsy most closely. xref Valko et al . reported only a small increase in the number of histamine cells in the Hcrt knockout mice, but not in the ataxin-3-hypocretin mouse. xref These data lead us to conclude that the large increase in histamine neurons in human patients with narcolepsy is not a compensation for the loss of hypocretin neurons."

sparser
"Interestingly, this increase in REM sleep during the subjective dark period was originally shown in orexin-ataxin-3 mice but not orexin knockout mice, which was thought to indicate an effect of the loss of orexin neurons as opposed to the loss of the orexin peptides per se (Kantor et al., 2009)."

sparser
"This manipulation also caused a marked decrease in time spent in cataplexy-like episodes when applied to narcoleptic orexin-ataxin-3 mice."
GJA8 affects ATXN3
| 3
| 3

sparser
"An autosomal dominant form of CAE was also present in the family pedigree, and it was demonstrated that an interaction of GABRG2 with another gene or genes is required for the CAE phenotype in this family ( xref )."

sparser
"Our findings expand the spectrum of Cx50 mutations that are associated with autosomal dominant congenital pulverulent nuclear cataract."

sparser
"139G>A) in GJA8 gene associated with autosomal dominant congenital cataract in a Chinese family."
GJA3 affects ATXN3
| 3
| 3

sparser
"559C>T) in the GJA3 gene associated with autosomal dominant pulverulent cataracts in a Chinese family."

sparser
"176C>T) in GJA3 gene associated with autosomal dominant congenital pulverulent cataract in a Chinese family."

sparser
"P59L) in GJA3 ( Cx46 ) associated with autosomal dominant pulverulent cataract in a Chinese family."
GEMIN4 affects ATXN3
| 3
| 3

reach
"Interestingly, mutation in the VBM motif of Atx3 100Q (Atx3 100Q / VBM *, 282 RKRR to HNHH), which disrupts the specific interaction between Atx3 and P97, significantly abolished the sequestration of P97 into aggregates; even the amount of P97 sequestered by the mutant was less than that by Atx3 22Q under the condition of similar amounts of the Atx3 aggregates (XREF_FIG)."

reach
"Because Atx3 binds P97 through a VBM motif XREF_BIBR, we applied isoform I of Atx3 (Atx3-I) as a molecular model to explore the relationship between sequestration and specific interaction (XREF_FIG)."

reach
"Moreover, Atx3 interacts with P97 and VCP through a VCP binding motif (VBM) XREF_BIBR XREF_BIBR."
GABARAP affects MAP1LC3C
| 3
| 3

sparser
"Ataxin-3 preferentially binds GABARAP and LC3C with either of its two recently identified LIR motifs."

sparser
"Moreover, Ataxin-3 interacts with the autophagosomal membrane proteins LC3C and GABARAP and transiently localized to early autophagosomes."

sparser
"ATXN3 interacts directly with GABARAP and LC3C."
FGFR3 affects ATXN3
| 3
| 3

sparser
"Specific germline point mutations affecting differing domains of the FGFR3 gene are associated with several autosomal dominant skeletal dysplasias: dwarfism and several craniosynostosis syndromes including hypochondroplasia, severe achondroplasia with developmental delay and acanthosis nigricans (SADDAN), thantophoric dysplasia, Crouzon syndrome with acanthosis nigricans, and coronal craniosynostosis. xref – xref The same mutations that are seen in these skeletal dysplasias have also been identified in several human cancers and thus it has also been suggested that FGFR3 mutations may be oncogenic xref ."

sparser
"Recently, a third autosomal dominant disorder, hypochondroplasia, has been associated with mutations in FGFR3 [21-23]."

sparser
"Specific mutations in the FGFR3 gene are associated with autosomal dominant human skeletal disorders such as hypochondroplasia, achondroplasia, and thanatophoric dysplasia."
FBN1 affects ATXN3
| 3
| 3

sparser
"Previous studies reported that the autosomal dominant form of WMS is caused by mutations in FBN1 xref , xref , while mutations in ADAMTS10 were shown to cause recessive WMS xref , xref ."

sparser
"This finding suggests that autosomal dominant non-syndromic TAAD may be associated with FBN1 gene mutation and implicates the necessity of echocardiographic screening of the relatives of patients with familial TAAD."

sparser
"VBD has been associated with aortic dilations, ectatic coronary arteries, Marfan syndrome, late-onset Pompe disease, autosomal dominant polycystic kidney disease, and Fabry disease ( xref )."
EPHA2 affects ATXN3
| 3
| 3

sparser
"EPHA2 belongs to the tyrosine kinase family of proteins and is an epithelial cell kinase that has been associated with autosomal dominant cataracts and recently it was implicated in age-related cortical cataracts in humans and mice [ xref – xref ]."

sparser
"EPHA2 belongs to the tyrosine kinase family of proteins and is an epithelial cell kinase that has been associated with autosomal dominant cataracts and recently it was implicated in age-related cortical cataracts in humans and mice [ xref , xref ]."

sparser
"Mutations in EPHA2 have been associated with autosomal dominant congenital/juvenile cataract and autosomal recessive congenital cataract."
DCM affects ATXN3
| 3
ATXN3 binds DCM. 3 / 3
| 3

sparser
"Here, we report that this mutation can cause autosomal dominant form of DCM."

sparser
"In an autosomal dominant form of DCM caused by mutations in the RNA‐binding motif protein 20 gene ( RBM20 ), for example, stage‐specific transcriptome profiling demonstrated early molecular perturbations during cardiogenesis in patient‐specific hiPSCs xref ; these results suggested that this clinically aggressive form of DCM is a developmental disorder."

sparser
"Several genes have been reported to cause the autosomal dominant form of DCM."
CSNK2B affects ATXN3
2 |
2 |

No evidence text available

No evidence text available
CSNK2A1 affects ATXN3
| 1
| 1

sparser
"ATX3 interacts with CK2α, while the pharmacological inhibition of CK2 reduces the amount of nuclear ATX3 levels as well as inclusions formation [ xref ]."
CEP290 affects ATXN3
| 3
| 3

sparser
"These include an autosomal dominant form of spastic paraplegia type 4 (SPG4=familial spastic paraplegia 2), hereditary essential tremor 2 (ETM2) and holoprosencephaly 2 (HPE2)."

sparser
"Mutations in these genes are found to be associated either with different forms of autosomal dominant Wolfram syndrome, autosomal recessive spinocerebellar ataxia-1/autosomal dominant juvenile amyotrophic lateral sclerosis type 4, or manifesting clinically as types of hemolytic anemia, features not observed in these isolated CE patients."

sparser
"The autosomal dominant form of the disease, type 4, is due to mutations in the SLC40A1 gene, which encodes for ferroportin (FPN)."
CDC48A affects ATXN3
| 2 1
| 2 1

reach
"Activation has also been reported for other deubiquitinating enzymes XREF_BIBR - XREF_BIBR and most recently, a synergistic cooperation between ataxin-3 and CDC-48 (C. elegans orthologue of VCP and p97) was found to have a role in ageing regulation and ubiquitin mediated proteolysis in C. elegans XREF_BIBR."

sparser
"CDC-48 binds to both ATX-3 and UBXN-5 in a non-competitive manner, suggesting the formation of a trimolecular complex."

reach
"CDC-48 binds to both ATX-3 and UBXN-5 in a non competitive manner, suggesting the formation of a trimolecular complex."
CASP3 affects ATXN3
| 3
| 3

sparser
"The most common configuration for the normal allele is (CAG) 8 CAA(-CAG) 4 CAA(CAG) 8 , xref whereas the most common SCA2 pathogenic allele contains 37 uninterrupted CAG trip-lets. xref The prevalence of SCA2 is 1–2/100,000, similar to that of another form of autosomal dominant ataxia, SCA1. xref However, significant geographic and ethnic variations exist, with the prevalence reaching 41/100,000 in the Holguin region of Cuba due to a possible founder effect. xref Recently, intermediate-length ATXN2 repeat expansions (27–33 triplets), mostly interrupted by 1 to 3 CAA triplets, have been associated with an increased risk for amyotrophic lateral sclerosis (ALS). xref "

sparser
"Moreover, up-regulation of atx2 , the Drosophila ortholog of the human gene that causes SCA2 disease, has been shown to enhance the toxicity of human disease forms of SCA1 and SCA3 in flies [ xref ]."

sparser
"These SCAs (SCA1SCA3, SCA6, SCA7, and SCA17) are clinically characterized by a progressive ataxia with cerebellar degeneration that in most of these SCAs consists of severe degeneration and loss of Purkinje cells, the major integrative neuron of the cerebellar cortex ( xref ; xref )."
CACNA1A affects ATXN3
| 1 2
| 1 2

sparser
"Mutations in CACNA1A are also associated with other autosomal dominant neurological disorders characterized by cerebellar dysfunction, such as EA2 (Ophoff et al. xref )."

reach
"We also analyzed the possibility of genetic interactions between ATXN1 and ATXN2, ATXN2 and ATXN3, and ATXN2 and CACNA1A."

sparser
"Several autosomal dominant human neurological disorders are associated with mutations in the CACNA1A gene including; familial hemiplegic migraine, generalized epilepsy with ataxia, episodic ataxia type 2 and spinocerebellar ataxia type-6."
C16orf70 affects ATXN3
2 1 |
2 1 |

No evidence text available

No evidence text available

No evidence text available
BLD-2736 affects ATXN3
| 3
BLD-2736 inhibits ATXN3. 3 / 3
| 3

reach
"We report that BLD-2736 improves the swimming of the SCA3 zebrafish and decreases the presence of ataxin-3 protein aggregates and the presence of full-length polyQ expanded human ataxin-3 protein in transgenic zebrafish."
| PMC

reach
"Treatment with BLD-2736 Decreases the Presence of Protein Aggregates and PolyQ Expanded Ataxin-3 in SCA3 Zebrafish."
| PMC

reach
"Whilst it may be questioned whether the treatment with BLD-2736 may have instead decreased full-length ataxin-3 through effects on the expression of EGFP-ataxin-3 via an effect on transcription, we previously demonstrated that treatment with calpeptin decreased the presence of full length human ataxin-3 protein, without altering the levels of human ataxin-3 mRNA, and that this effect was prevented by cotreatment with the autophagy inhibitor, chloroquine [43]."
| PMC
ATXN3 affects terC
| 3
| 3

sparser
"Interestingly, the existence of oxidative stress in lymphocytes from patients with an autosomal dominant form of DC with mutations in TERC has been recently reported xref ."

sparser
"This theory was supported by the description of an autosomal dominant form of DC, caused by mutations in TERC [ xref ]."

sparser
"Further analysis indicated that transcriptional silencing owing to a 3′ deletion in the TERC locus leads to the autosomal dominant form of dyskeratosis congenita by diminishing TERC transcription."
ATXN3 affects smo-1
| 1 2
| 1 2

sparser
"Using a conventional immunoprecipitation method, we could not detect SUMO-modified form of ataxin-3 (Jung & Lee xref )."

reach
"To address whether ataxin-3 can associate with SUMO, we used recombinant SUMO1 or SUMO2 conjugated to beads to perform pull-down assays from cell lysates."

sparser
"While ATXN3 can bind SUMO and be SUMOylated ( xref ; xref ), it is not yet clear how SUMO binding or SUMOylation alters ATXN3 function or clearance."
ATXN3 affects pathway
| 3
ATXN3 activates pathway. 3 / 3
| 3

sparser
"In addition, they found that expanded ataxin3 could activate the mitochondrial apoptotic pathway and induce neuronal death by regulating gene expression [ xref ]."

sparser
"Moreover, mutant ATX3 activates pro-apoptotic pathway by activating ATM, which can be restored by ATM inhibition (Gao et al., xref )."

sparser
"Besides, mutant ATX3 activates apoptosis pathway mediated by p53 and PKC after prolonged accumulation of DNA damage (Gao et al., xref )."
ATXN3 affects p97
| 3
ATXN3 binds p97. 3 / 3
| 3

sparser
"Because Atx3 binds P97 through a VBM motif xref , we applied isoform I of Atx3 (Atx3-I) as a molecular model to explore the relationship between sequestration and specific interaction ( xref )."

sparser
"Interestingly, mutation in the VBM motif of Atx3 100Q (Atx3 100Q /VBM*, 282 RKRR to HNHH), which disrupts the specific interaction between Atx3 and P97, significantly abolished the sequestration of P97 into aggregates; even the amount of P97 sequestered by the mutant was less than that by Atx3 22Q under the condition of similar amounts of the Atx3 aggregates ( xref )."

sparser
"Although it cannot be excluded that the lower amount of the aggregates may have some correlation to the decreased sequestration, we think that the major contribution to the decreased ability of the VBM mutant to sequester P97 is from disruption of the specific interaction between Atx3 100Q and P97."
ATXN3 affects VHL
| 3
| 3

sparser
"VHL disease, an autosomal dominant disorder, is associated with structural abnormalities on chromosome 3p and is characterized by a predisposition to a variety of neoplasms, including RCC."

sparser
"The most common subtype, clear cell RCC, is associated with autosomal dominant mutation of the Von Hippel-Lindau gene (VHL).[ xref ] Smoking and obesity are other known risk factors.[ xref ] Papillary RCC is the second most common subtype."

sparser
"Mutations in VHL are associated with lung cancer, sporadic clear cell renal carcinomas and an autosomal dominant familial cancer known as von Hippel-Lindau disease ([ xref - xref ] and see [ xref ])."
ATXN3 affects UMOD
| 3
| 3

sparser
"Mutations in the UMOD gene are associated with medullary cystic kidney disease and autosomal dominant tubulointerstitial kidney disease [ xref , xref ]."

sparser
"The role of UMOD in normal kidney function is incompletely understood, however, mutations of the UMOD gene are associated with autosomal dominant tubulointerstitial kidney disease (ADTKD- UMOD ) which as previously been known as familial juvenile hyperuricaemic nephropathy (FJHN) and medullary cystic kidney disease (MCKD2), also known as UMOD-associated kidney diseases [ xref , xref , xref , xref , xref ]."

sparser
"It should be noted, however, that irrespective of genetic phenotypes, in both CHD populations and cohorts with established Autosomal Dominant Tubulointerstitial Kidney Disease (ADTKD-UMOD), serum uromodulin concentrations were independently associated with hard outcomes in the former [ xref ], or were lower in all affected patients with a novel missense mutation in the UMOD gene (457T>G; Cys153Gly), relative to all patients of the reference eGFR-matched CKD groups, and healthy, unaffected family members [ xref ]."
ATXN3 affects UBQLN1
1 1 | 1
1 1 | 1

No evidence text available

No evidence text available

reach
"Here, we show that PLIC-1 also binds to the UIM proteins ataxin 3 -- a deubiquitinating enzyme -- HSJ1a -- a co-chaperone -- and EPS15 (epidermal growth factor substrate 15) -- an endocytic protein."
ATXN3 affects TRIP11
| 3
| 3

sparser
"B4GALNT1 , G2E3-SCFD1 , and TRIP11-ATXN3 SNPs did not reach genome-wide significance, but the genes achieved significance in a gene-based analysis that combines all SNP association signals within a gene."

sparser
"We have identified one additional ALS locus, ACSL5-ZDHHC6 , and three additional putative loci, B4GALNT1 , G2E3-SCFD1 , and TRIP11-ATXN3 , with potential functional relevance for ALS."

sparser
"Moreover, B4GALNT1, G2E3-SCFD1, and TRIP11-ATXN3 are identified using a gene-based analysis."
ATXN3 affects STK11
| 3
| 3

sparser
"However, oligonucleotide aCGH demonstrated a de novo 1.1-Mb deletion of 19p13.3 including STK11 , which is associated with autosomal dominant Peutz-Jeghers syndrome (PJS)( xref )."

sparser
"Liver kinase B1 (LKB1, STK11) is key regulatory protein of cellular metabolism that was originally identified in patients with Peutz-Jeghers syndrome (PJS), an autosomal dominant disease associated with LKB1 germline alterations xref ."

sparser
"Loss-of-function germline mutations of LKB1 are associated with Peutz–Jeghers syndrome (PJS), an autosomal dominant disorder."
ATXN3 affects SIGE
| 3
| 3

sparser
"The autosomal dominant hyper IgE syndrome is associated with a missense or in-frame deletions in the SH2 and DNA-binding domains in the STAT3 gene (Signal Transducer and Activator of Transcription 3) [ xref ]."

sparser
"Indeed, patients with the autosomal dominant form of hyper IgE syndrome (HIES) have severely reduced numbers of IL-17 producing circulating T cells due to dominant-negative mutations of the signal transducer and activator of transcription 3 ( STAT3 ), and often suffer from CMC [ xref – xref ]."

sparser
"DOCK8 deficiency gives rise to an autosomal recessive form of the hyper IgE (AR-HIES) syndrome that overlaps in its features with autosomal dominant hyper IgE (AD-HIES) syndrome caused by loss of function mutations in STAT3 ( xref – xref )."
ATXN3 affects SCN5A
| 3
| 3

sparser
"Brugada syndrome is a life-threatening, inherited arrhythmia disorder associated with autosomal dominant mutations in SCN5A [ xref - xref ], the gene encoding the human cardiac Na + channel α subunit (Nav1.5) [ xref ], which contains four homologous domains, each composed of six membrane-spanning segments, linked by cytoplasmic linkers."

sparser
"Brugada syndrome (BrS) is a life-threatening, inherited arrhythmogenic syndrome associated with autosomal dominant mutations in SCN5A , the gene encoding the cardiac Na + channel alpha subunit (Na v 1.5)."

sparser
"Brugada syndrome is a life-threatening, inherited arrhythmia disorder associated with autosomal dominant mutations in SCN5A, the gene encoding the human cardiac Na + channel α subunit (Nav1.5)."
ATXN3 affects SCD
| 3
| 3

sparser
"Autosomal dominant forms of SCD have also been reported, but to date no genetic etiology has been described for these."

sparser
"This is the first identification of the genetic cause of an autosomal dominant form of SCD, and also demonstrates the potential of exome sequencing to identify genetic causes of dominant diseases even in small families with few affected individuals."

sparser
"Recently, an autosomal dominant form of SCD has been attributed to TBX6 mutation [ xref ]."
ATXN3 affects RAD23 family, and VCP
| 3
| 3

sparser
"Ataxin-3 interacts with ubiquitinated substrates, p97, Rad23, and the proteasome, and the expanded-polyglutamine-containing ataxin-3 is defective in the substrate degradation [74] ."

sparser
"Ataxin-3 binds directly to at least two PQC-related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; xref )."

sparser
"Ataxin-3 binds directly to at least two PQC-related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; Fig. 1 A)."
ATXN3 affects PTCH1
| 3
| 3

sparser
"Importantly, TSPAN8 overexpression enhanced the binding of ATXN3 to PTCH1 in MCF-7 cells, whereas TSPAN8 depletion reduced their interaction (Fig.  xref )."

sparser
"These results suggest that TSPAN8 facilitates the binding of ATXN3 to PTCH1 to reduce the degradation of PTCH1."

sparser
"These results indicate that TSPAN8 stabilizes PTCH1 expression by promoting the binding of ATXN3 to PTCH1, leading to deubiquitylation of PTCH1."
ATXN3 affects PSMD4
| 3
| 3

sparser
"This study describes a previously unreported autosomal dominant form of heritable AF, with early age of onset, associated conduction system disease and atrial myopathy."

sparser
"In pull-down assays, ubiquitination of CHIP enhanced its interaction with both S5a and ataxin-3 ( xref )."

sparser
"The identification of a single genetic variant that associates with the autosomal dominant AF subtype, in more than one unrelated family, would represent a significant breakthrough in understanding the genetics and molecular mechanisms for the manifestation of AF."
ATXN3 affects PRSS1
| 3
| 3

sparser
"Germline mutations in the PRSS1 gene, which encodes cationic trypsinogen, has been associated with an autosomal dominant form of hereditary pancreatitis."

sparser
"The most common cause of hereditary pancreatitis is an autosomal dominant form caused by mutations in the PRSS1 gene which account for up to 80% of cases [ xref ]."

sparser
"Heterozygous PRSS1 mutations are often associated with autosomal dominant hereditary disease, indicating that these are strong, essentially causative genetic changes [ xref , xref and references therein]."
ATXN3 affects PPK
| 3
ATXN3 binds PPK. 3 / 3
| 3

sparser
"Taken together, these results suggest that CDH12 gene is a potential candidate gene for an atypical presentation of an autosomal dominant form of transgrediens and progrediens PPK."

sparser
"In this study, we identified five families of Pakistani origin with an autosomal dominant form of PPK."

sparser
"We investigated at the clinical and genetic level a consanguineous Tunisian family presenting an autosomal dominant atypical form of transgrediens and progrediens PPK to better characterize this ultrarare disease and to identify its molecular etiology."
ATXN3 affects PABPN1
| 3
| 3

sparser
"Initial symptoms in terms of dysphagia appeared at the age of 70, much later than the usual disease onset of the autosomal dominant form of OPMD, which usually becomes symptomatic at the age between 40 and 60 years."

sparser
"A hallmark of the autosomal dominant form of OPMD is the presence of nuclear aggregates in muscle tissue [ xref ] similar to other polyalanine and polyglutamine diseases [ xref – xref ]."

sparser
"Patient 2 (G.F.) was a 63 year old man with a clinically diagnosed autosomal dominant form of OPMD."

reach
"Moreover, various gain/loss functional assays were performed to indicate that ATXN3 overexpression enhanced ATC cell proliferation and metastasis."

reach
"Furthermore, we demonstrated that ATXN3 promoted breast cancer cell metastasis via KLF4 in vitro and in vivo."

reach
"We also found that ATXN3 and eukaryotic translation initiation factor 5A2 (EIF5A2) protein levels in ATC tissues are positively correlated, and ATXN3 promotes the proliferation and metastasis of ATC cells through EIF5A2."
ATXN3 affects NOTCH3
| 3
| 3

sparser
"Moreover, Notch3 mutation is associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) [ xref , xref ]."

sparser
"NOTCH3 has been associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), a dementia disorder which clinical phenotype overlaps with EOAD."

sparser
"Mutations in human NOTCH3 are associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), a disorder that causes stroke and dementia and is accompanied by the degeneration of vascular smooth muscle cells [ xref ]; adult Notch3 mutant mice display a defect in the maturation of arterial smooth muscle cells [ xref ]."
ATXN3 affects Machado-Joseph disease
| 3
ATXN3 activates Machado-Joseph disease. 3 / 3
| 3

reach
"Expansion of a polyglutamine tract in ATXN3 causes Machado-Joseph disease, a late-onset neurodegenerative disorder characterized by ubiquitin positive aggregate formation and specific neuronal death."

reach
"Mutant HTT causes Huntington 's disease (HD) XREF_BIBR, XREF_BIBR and mutant ATX-3 causes Machado-Joseph disease (MJD)."

reach
"A skin biopsy of a patient with spinocerebellar ataxia type 3 (SCA3, also known as Machado-Joseph disease (MJD)) caused by a CAG trinucleotide repeat expansion in the ATXN3 gene, was used to generate an induced pluripotent stem cell line, HIHCNi002-A (iPSC-SCA3)."
ATXN3 affects MYC
| 3
| 3

sparser
"Reciprocally, Myc-ATXN3 co-IPed both ubiquitin-free Flag-Rheb and Ub-Flag-Rheb ( xref )."

sparser
"Notably, the expression of Myc-ATXN3 but not its enzyme-inactive mutant (C14A) reduced levels of Ub-Rheb in the lysate ( xref and xref )."

sparser
"Flag-Rheb but not Flag-Rap2a, which is also expressed on the lysosome ( xref ), specifically co-IPed Myc-ATXN3 ( xref )."
ATXN3 affects MBNL1
| 3
| 3

sparser
"Pentameric 5H-4 Potently Inhibits Formation of the SCA3 RNA-MBNL1 Interaction."

sparser
"As mentioned above, both MBNL1 and 5H-4 bind to RNA12 , which contains 12 copies of the 5′C A G/3′G A C motif that is present in toxic SCA3 repeats. xref Recently, the interaction of SCA3 RNAs with Muscleblind proteins has been implicated in the disease pathology of spinocerebellar ataxia type 3. xref Therefore, we sought to study the ability of 5H-4 to inhibit SCA3 RNA-MBNL1 interactions."

sparser
"Herein, we describe the design of cell permeable, modularly assembled ligands that inhibit the formation of the DM1 RNA- and SCA3 RNA-MBNL1 interactions with low nanomolar IC 50 's."
ATXN3 affects MAP1LC3C
| 3
| 3

sparser
"Ataxin-3 preferentially binds GABARAP and LC3C with either of its two recently identified LIR motifs."

sparser
"Moreover, Ataxin-3 interacts with the autophagosomal membrane proteins LC3C and GABARAP and transiently localized to early autophagosomes."

sparser
"ATXN3 interacts directly with GABARAP and LC3C."
ATXN3 affects LY6E
| 3
ATXN3 activates LY6E. 3 / 3
| 3

reach
"SCA2 and SCA3 are caused by polyglutamine expansions in ataxin2 and ataxin3, respectively [XREF_BIBR, XREF_BIBR]."

reach
"XREF_BIBR SCA1, SCA2, Machado-Joseph or SCA3, SCA6, SCA7, SCA12, SCA17, and dentatorubral-pallidoluysian atrophy (DRPLA) are caused by (CAG) n repeat expansions in the ATXN1, ATXN2, ATXN3, CACNA1A, ATXN7, PPP2R2B, TBP, and ATN1 genes, respectively, and all lead to the expansion of a polyglutamine tract in the corresponding proteins."

reach
"Ataxin gene variants in ATXN1, ATXN2, ATXN3, and ATXN7 cause SCA1, SCA2, SCA3, and SCA7, respectively."
ATXN3 affects KAT2B
2 | 1
2 | 1

No evidence text available

No evidence text available

reach
"showed that Atxn3 interacted with three major histone acetyltransferases, CBP, p300, and p300 and CBP associated factor and inhibited their acetyltransferase activity resulting in repression of model gene promoters."
ATXN3 affects K48-, and K63
| 3
ATXN3 binds K48- and K63. 3 / 3
| 3

reach
"In vitro, ataxin-3 binds K48- and K63 linked Ub chains at least four Ub long through its UIMs, preferentially cleaves chains longer than four Ub, and cleaves K63-linkages better than K48-ones [XREF_BIBR, XREF_BIBR]."

reach
"Interestingly, ataxin-3 binds to both K48- and K63 linked chains, but preferentially cleaves the latter chain type (Winborn et al., 2008)."

reach
"Mutant (expanded) ATXN3 still binds K48- and K63 linked polyUb chains, gets activated by ubiquitination, and retains DUB catalytic activity in vitro against K48 and K63 chains similarly to normal ATXN3, but expanded ATXN3 does appear to have an enhanced capacity to deubiquitinate K27- and K29 linked Ub chains."
ATXN3 affects Interferon
| 3
| 3

reach
"Our study identified both STUB1 and ATXN3 also function as negative regulators of both RIG-I signaling and IFN signaling further supporting a critical functional coupling of RIG-I and IFN signaling and the UPR."

reach
"ATXN3 enhances type 1 IFN signaling during viral infection through deubiquitinating and stabilizing HDAC3 [90]."

reach
"Unlike USP2A, the deubiquitinating enzymes BRCC36, USP13, and USP39 positively regulate IFN activities by attenuating the polyubiquitination level of STAT1, and this process is independent of IFN treatment, which suggests divergent functional roles of these DUBs under differential contexts.Additionally, ATXN3 does not affect IFN-I production during viral infection but positively regulates IFNAR1-mediated downstream signaling by targeting HDAC3 (108)."
ATXN3 affects ITCH
2 | 1
2 | 1

No evidence text available

No evidence text available

sparser
"We observed only the interaction of ITCH with expanded-polyglutamine ataxin-3(84Q) proteins ( xref )."
ATXN3 affects HTRA1
| 3
| 3

sparser
"Recently, other heterogeneous autosomal dominant disorders have been associated with heterozygous HTRA1 gene mutations [ xref ]."

sparser
"For instance, an état criblée of the basal ganglia may be more prevalent in HTRA1 autosomal dominant related forms [ xref ]."

sparser
"The few studies on the relative frequency of these disorders indicate that the most frequent (or rather the least rare), accounting for more than half of patients, is CADASIL, due to mutations of the NOTCH3 gene, followed by COL4A1/A2-related disease, autosomal dominant forms of HTRA1-related disease and leucoencephalopathies with calcifications and cysts."
ATXN3 affects HSPA8
| 3
| 3

sparser
"The model above focuses on the role of the UIMs facilitating an interaction between ataxin-3 and Hsc70-4, because pathogenic ataxin-3 with mutated UIMs does not co-IP Hsc70-4."

sparser
"UIMs mediate ataxin-3 interaction with Hsc70-4."

sparser
"It may also be that under normal circumstances ataxin-3 forms a functional complex with Hsc70-4, collaborating in protein quality control."
ATXN3 affects HSJ1a
| 1 2
ATXN3 binds HSJ1a. 3 / 3
| 1 2

reach
"Next, we tested whether HSJ1a, CHIP, HSP70 and Atx3 can form a complex in cells."

sparser
"HSJ1a binds ubiquitinated Atx3 and retards its degradation."

sparser
"As known, ubiquitination of a protein substrate is required for its proteasomal degradation xref , xref , thus the degradation of Atx3 modulated by HSJ1a may also be associated with ubiquitination of Atx3."
ATXN3 affects HDAC3, and NCOR1
| 3
| 3

sparser
"Both normal (Q23) and expanded (Q70) human full-length Ataxin-3 physiologically interacted with HDAC3 and NCoR in a SCA3 rat cell line and human pons tissue."

sparser
"A direct interaction of ATXN3 with HDAC3 and NCoR has previously been established in SCA3 cell lines and human brain extracts yet with only normal (i.e. not expanded) ATXN3 being associated with increased deacetylase activity and repression of gene expression [ xref ]."

sparser
"Further, it was shown that both normal and expanded ataxin 3 physiologically interact with HDAC3 and NCoR in a SCA3 cell model and in human pons tissue; however, normal ataxin 3-containing protein complexes showed increased histone deacetylase activity, whereas polyglutamine-expanded ataxin 3-containing complexes had reduced deacetylase activity in target chromatin regions [ xref ]."
ATXN3 affects HCRT
| 3
| 3

sparser
"A robust increase (64–94%) in the number of histamine neurons in the TMN has been reported by two independent groups ( xref ). xref , xref However, we did not observe this increase of histamine neurons in a series of narcoleptic animal models we tested (hypocretin 2R mutant dogs, Hcrt knock-out mice, ataxin-3-hypocretin mice, and doxycycline-controlled diphtheria toxin A–hypocretin mice). xref Of these animal models, the postnatal hypocretin cell loss and adult-onset symptoms seen in the doxycycline-controlled diphtheria toxin A–hypocretin mouse resembles the clinical characteristics human narcolepsy most closely. xref Valko et al . reported only a small increase in the number of histamine cells in the Hcrt knockout mice, but not in the ataxin-3-hypocretin mouse. xref These data lead us to conclude that the large increase in histamine neurons in human patients with narcolepsy is not a compensation for the loss of hypocretin neurons."

sparser
"Interestingly, this increase in REM sleep during the subjective dark period was originally shown in orexin-ataxin-3 mice but not orexin knockout mice, which was thought to indicate an effect of the loss of orexin neurons as opposed to the loss of the orexin peptides per se (Kantor et al., 2009)."

sparser
"This manipulation also caused a marked decrease in time spent in cataplexy-like episodes when applied to narcoleptic orexin-ataxin-3 mice."
ATXN3 affects GJA8
| 3
| 3

sparser
"An autosomal dominant form of CAE was also present in the family pedigree, and it was demonstrated that an interaction of GABRG2 with another gene or genes is required for the CAE phenotype in this family ( xref )."

sparser
"Our findings expand the spectrum of Cx50 mutations that are associated with autosomal dominant congenital pulverulent nuclear cataract."

sparser
"139G>A) in GJA8 gene associated with autosomal dominant congenital cataract in a Chinese family."
ATXN3 affects GJA3
| 3
| 3

sparser
"559C>T) in the GJA3 gene associated with autosomal dominant pulverulent cataracts in a Chinese family."

sparser
"176C>T) in GJA3 gene associated with autosomal dominant congenital pulverulent cataract in a Chinese family."

sparser
"P59L) in GJA3 ( Cx46 ) associated with autosomal dominant pulverulent cataract in a Chinese family."
ATXN3 affects GABARAP, and MAP1LC3C
| 3
| 3

sparser
"Ataxin-3 preferentially binds GABARAP and LC3C with either of its two recently identified LIR motifs."

sparser
"Moreover, Ataxin-3 interacts with the autophagosomal membrane proteins LC3C and GABARAP and transiently localized to early autophagosomes."

sparser
"ATXN3 interacts directly with GABARAP and LC3C."
ATXN3 affects FGFR3
| 3
| 3

sparser
"Specific germline point mutations affecting differing domains of the FGFR3 gene are associated with several autosomal dominant skeletal dysplasias: dwarfism and several craniosynostosis syndromes including hypochondroplasia, severe achondroplasia with developmental delay and acanthosis nigricans (SADDAN), thantophoric dysplasia, Crouzon syndrome with acanthosis nigricans, and coronal craniosynostosis. xref – xref The same mutations that are seen in these skeletal dysplasias have also been identified in several human cancers and thus it has also been suggested that FGFR3 mutations may be oncogenic xref ."

sparser
"Recently, a third autosomal dominant disorder, hypochondroplasia, has been associated with mutations in FGFR3 [21-23]."

sparser
"Specific mutations in the FGFR3 gene are associated with autosomal dominant human skeletal disorders such as hypochondroplasia, achondroplasia, and thanatophoric dysplasia."
ATXN3 affects FBN1
| 3
| 3

sparser
"Previous studies reported that the autosomal dominant form of WMS is caused by mutations in FBN1 xref , xref , while mutations in ADAMTS10 were shown to cause recessive WMS xref , xref ."

sparser
"This finding suggests that autosomal dominant non-syndromic TAAD may be associated with FBN1 gene mutation and implicates the necessity of echocardiographic screening of the relatives of patients with familial TAAD."

sparser
"VBD has been associated with aortic dilations, ectatic coronary arteries, Marfan syndrome, late-onset Pompe disease, autosomal dominant polycystic kidney disease, and Fabry disease ( xref )."
ATXN3 affects EPHA2
| 3
| 3

sparser
"EPHA2 belongs to the tyrosine kinase family of proteins and is an epithelial cell kinase that has been associated with autosomal dominant cataracts and recently it was implicated in age-related cortical cataracts in humans and mice [ xref – xref ]."

sparser
"EPHA2 belongs to the tyrosine kinase family of proteins and is an epithelial cell kinase that has been associated with autosomal dominant cataracts and recently it was implicated in age-related cortical cataracts in humans and mice [ xref , xref ]."

sparser
"Mutations in EPHA2 have been associated with autosomal dominant congenital/juvenile cataract and autosomal recessive congenital cataract."
ATXN3 affects EFNA3
| 3
ATXN3 decreases the amount of EFNA3. 3 / 3
| 3

reach
"These results indicate that ATXN3 represses Efna3 transcription by acting, either directly or indirectly, on the promoter region."

reach
"Both normal and expanded ATXN3 significantly repressed Efna3 transcription by approximately 20% but had no effect on expression of the control pGL3 luciferase vector (XREF_FIG)."

reach
"To identify which histone acetylases and regulators are likely involved in establishing the histone acetylation and enhanced gene expression of Efna3 induced by the loss of ATXN3, we measured their nuclear protein levels in both Atxn3-KO and WT cells."
ATXN3 affects DCM
| 3
ATXN3 binds DCM. 3 / 3
| 3

sparser
"Here, we report that this mutation can cause autosomal dominant form of DCM."

sparser
"In an autosomal dominant form of DCM caused by mutations in the RNA‐binding motif protein 20 gene ( RBM20 ), for example, stage‐specific transcriptome profiling demonstrated early molecular perturbations during cardiogenesis in patient‐specific hiPSCs xref ; these results suggested that this clinically aggressive form of DCM is a developmental disorder."

sparser
"Several genes have been reported to cause the autosomal dominant form of DCM."
ATXN3 affects Caspase
| 2 1
ATXN3 activates Caspase. 3 / 3
| 2 1

reach
"The data with zVAD-fmk indicated that Ataxin-3 is a target of caspases also in Drosophila cells."

reach
"While it is reasonable to hypothesize that expanded Ataxin-3 protein may activate caspases that proteolyze Ataxin-3, the observation that normal Ataxin-3, which does not cause neurodegeneration, is also cleaved, suggests that cleavage is not necessarily a consequence of apoptosis."

sparser
"While it is reasonable to hypothesize that expanded Ataxin-3 protein may activate caspases that proteolyze Ataxin-3, the observation that normal Ataxin-3, which does not cause neurodegeneration, is also cleaved, suggests that cleavage is not necessarily a consequence of apoptosis."
ATXN3 affects CSNK2B
2 |
2 |

No evidence text available

No evidence text available
ATXN3 affects CSNK2A1
| 1
| 1

sparser
"ATX3 interacts with CK2α, while the pharmacological inhibition of CK2 reduces the amount of nuclear ATX3 levels as well as inclusions formation [ xref ]."
ATXN3 affects CEP290
| 3
| 3

sparser
"These include an autosomal dominant form of spastic paraplegia type 4 (SPG4=familial spastic paraplegia 2), hereditary essential tremor 2 (ETM2) and holoprosencephaly 2 (HPE2)."

sparser
"Mutations in these genes are found to be associated either with different forms of autosomal dominant Wolfram syndrome, autosomal recessive spinocerebellar ataxia-1/autosomal dominant juvenile amyotrophic lateral sclerosis type 4, or manifesting clinically as types of hemolytic anemia, features not observed in these isolated CE patients."

sparser
"The autosomal dominant form of the disease, type 4, is due to mutations in the SLC40A1 gene, which encodes for ferroportin (FPN)."
ATXN3 affects CDC48A
| 2 1
| 2 1

reach
"Activation has also been reported for other deubiquitinating enzymes XREF_BIBR - XREF_BIBR and most recently, a synergistic cooperation between ataxin-3 and CDC-48 (C. elegans orthologue of VCP and p97) was found to have a role in ageing regulation and ubiquitin mediated proteolysis in C. elegans XREF_BIBR."

sparser
"CDC-48 binds to both ATX-3 and UBXN-5 in a non-competitive manner, suggesting the formation of a trimolecular complex."

reach
"CDC-48 binds to both ATX-3 and UBXN-5 in a non competitive manner, suggesting the formation of a trimolecular complex."
ATXN3 affects CACNA1A
| 1 2
| 1 2

sparser
"Mutations in CACNA1A are also associated with other autosomal dominant neurological disorders characterized by cerebellar dysfunction, such as EA2 (Ophoff et al. xref )."

reach
"We also analyzed the possibility of genetic interactions between ATXN1 and ATXN2, ATXN2 and ATXN3, and ATXN2 and CACNA1A."

sparser
"Several autosomal dominant human neurological disorders are associated with mutations in the CACNA1A gene including; familial hemiplegic migraine, generalized epilepsy with ataxia, episodic ataxia type 2 and spinocerebellar ataxia type-6."
ATXN3 affects C16orf70
2 1 |
2 1 |

No evidence text available

No evidence text available

No evidence text available
ATXN3 affects BAX
| 2
ATXN3 increases the amount of BAX. 2 / 3
| 2

reach
"p53 activation mediates polyglutamine expanded ataxin-3 upregulation of Bax expression in cerebellar and pontine nuclei neurons."

reach
"In the present study, cellular and animal models of SCA3 were used to test the hypothesis that mutant polyglutamine ataxin-3 upregulates Bax expression of cerebellar and pontine nuclei neurons by augmenting transcriptional activity of p53."
ATXN3 affects ACTN4
| 3
| 3

sparser
"Mutations in ACTN4 are associated with an autosomal dominant form of familial FSGS (OMIM 603278)."

sparser
"Missense mutations in ACTN4 are associated with incompletely penetrant and late-onset autosomal dominant (AD) FSGS [ xref ]."

sparser
"In this paper, we focus on mutations in α -actinin-4, which are associated with an autosomal dominant late-onset FSGS in humans [ xref ]."
ATXN3 affects AAA ATPase
| 3
ATXN3 binds AAA ATPase. 3 / 3
| 3

reach
"Ataxin-3 binds directly to at least two PQC related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; Fig. 1 A)."

reach
"Ataxin-3 binds directly to at least two PQC related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; XREF_FIG)."

reach
"VCP is a ubiquitous, homo-hexameric AAA ATPase that is bound directly at its N-terminus by ataxin-3 through the VBM (Boeddrich et al., 2006; Buchberger et al., 2010) (Fig. 1A)."
ATXN2 affects ITPR1
| 3
ATXN2 binds HTT, ITPR1, and ATXN3. 3 / 3
| 3

sparser
"Importantly, mutant HTT, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of IP3R1 in rat neurons and increase its sensitivity to InsP3 [ xref , xref ]."

sparser
"My laboratory discovered that mutant Huntingtin, ataxin-2 and ataxin-3 proteins specifically binds to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP 3 R1), an intracellular Ca 2+ release channel."

sparser
"Our laboratory discovered that mutant huntingtin, ataxin-2 and ataxin-3 proteins specifically bind to the carboxy-terminal region of the type 1 inositol 1,4,5-trisphosphate receptor (IP(3)R1), an intracellular Ca(2+) release channel."
ATXN2 affects ATXN3, ATXN7, and MID1
| 3
| 3

sparser
"Furthermore, we show that this binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA, similar to what we have shown previously for HTT (Krauss et al., xref ), induces translation in a CAG repeat length-dependent manner in vitro and in cell lines."

sparser
"To test if the flanking regions influence the binding of MID1 to CAG repeat mRNAs, we tested the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNAs."

sparser
"Furthermore, we show that functionally, in line with what we have previously observed for HTT, the binding of MID1 to ATXN2, ATXN3 , and ATXN7 mRNA induces protein synthesis in a repeat length-dependent manner."
ACTN4 affects ATXN3
| 3
| 3

sparser
"Mutations in ACTN4 are associated with an autosomal dominant form of familial FSGS (OMIM 603278)."

sparser
"Missense mutations in ACTN4 are associated with incompletely penetrant and late-onset autosomal dominant (AD) FSGS [ xref ]."

sparser
"In this paper, we focus on mutations in α -actinin-4, which are associated with an autosomal dominant late-onset FSGS in humans [ xref ]."
AAA ATPase affects ATXN3
| 3
ATXN3 binds AAA ATPase. 3 / 3
| 3

reach
"Ataxin-3 binds directly to at least two PQC related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; Fig. 1 A)."

reach
"Ataxin-3 binds directly to at least two PQC related proteins, the AAA ATPase VCP (also known as p97) and Rad23 (orthologous to hHR23A and B in mammals; XREF_FIG)."

reach
"VCP is a ubiquitous, homo-hexameric AAA ATPase that is bound directly at its N-terminus by ataxin-3 through the VBM (Boeddrich et al., 2006; Buchberger et al., 2010) (Fig. 1A)."
Valproate affects ATXN3
| 2
Valproate increases the amount of ATXN3. 2 / 2
| 2

reach
"We also identified that valproate treatment produced a dose dependent increase in the amount of full-length human ataxin-3 present compared to in vehicle treated EGFP-Ataxin-3 84Q zebrafish (up to nearly twofold for high dose valproate; Fig. 1H)."

reach
"Valproate treatment (3 mM) produced significant increases in both ac-H3K9 and ac-H4K5 levels (Fig. 2B, C) and full-length human ataxin-3 levels, compared to vehicle controls (Fig. 2D), in a similar manner to the zebrafish findings."

reach
"Because atx3 binds the ubiquitin like domain of rad23, which also mediates the interaction between rad23 and the proteasome, it is likely that rad23 may not interact with atx3 and the proteasome simultaneously."

reach
"Moreover, ataxin-3 contains two ubiquitin interacting motifs [16] and binds to the ubiquitin like domain in the DNA repair proteins HHR23A and HHR23B [17]."

sparser
"Ataxin-3 normally interacts with numerous transcriptional regulators including the forkhead box O (FOXO)-4 transcription factor, TATA-binding protein-associated factor TAFII130 [ xref ], CBP [ xref ], nuclear co-repressor receptor NCoR [ xref ], histone deacetylases [ xref ], and DNA repair protein RAD23 [ xref ]."

sparser
"These observations suggest that next to ubiquitin-proteasomal regulation of transcription, endogenous ataxin-3 might also directly interact with important transcriptional regulators."
RpoB affects ATXN3
| 2
| 2

sparser
"The autosomal dominant form of STL is caused by mutations in COL2A1 [ xref ], COL11A1 [ xref ], or COL11A2 (Gene ID 1302, OMIM) [ xref ], while the autosomal recessive form of STL is caused by mutations in COL9A1 (Gene ID 12839, OMIM) [ xref ], COL9A2 (Gene ID 1298, OMIM) [ xref ], COL9A3 (Gene ID 1299, OMIM) [ xref ], or LOXL3 (Gene ID 84695, OMIM) [ xref , xref ]."

sparser
"Autosomal dominant forms of STL include type I (membranous vitreous) [STL1] caused by mutation in the COL2A1 gene, type II (beaded vitreous form by COL11A1 mutation), and type III (nonocular by COL11A2 mutation)."
RibE affects ATXN3
| 2
| 2

sparser
"Mutations in the ROR2 gene cause autosomal recessive RS (RRS) whereas mutations in WNT5A are responsible for the autosomal dominant (AD) form of RS."

sparser
"Mutations in WNT5A, locus 3p14.3 (OMIM 164975) are responsible for the autosomal dominant (AD) form of RS In these patients, oral manifestations are more prominent, hemivertebrae and scoliosis rarely occur and facial abnormalities tend to be milder ( xref )."
Resveratrol affects ATXN3
1 | 1
1 | 1

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reach
"The results show that resveratrol decreases the motor deficits and imbalance of MJD mice observed in stationary and accelerated rotarod tests (XREF_FIG), and also in beam walking test (XREF_FIG)."
Poly-ubiquitin chains affects ATXN3
| 2
ATXN3 binds poly-ubiquitin chains. 2 / 2
| 2

reach
"Ataxin-3 binds and cleaves poly-ubiquitin chains XREF_BIBR - XREF_BIBR."

reach
"The presence of two ubiquitin binding sites explains how ataxin-3 binds poly-ubiquitin chains and provides new insights into the molecular mechanism of ubiquitin recognition."
Phenylmercury acetate decreases the amount of ATXN3. 2 / 2
2 |

No evidence text available

No evidence text available
Monoubiquitin affects ATXN3
| 1 1
ATXN3 binds monoubiquitin. 2 / 2
| 1 1

sparser
"Atx3 can also bind monoubiquitin, and it does so through the multiple binding sites distributed along its length, albeit with low (20–400 μM) affinity."

reach
"Atx3 can also bind monoubiquitin, and it does so through the multiple binding sites distributed along its length, albeit with low (20-400 muM) affinity."
Magnetite nanoparticle decreases the amount of ATXN3. 2 / 2
2 |

No evidence text available

No evidence text available

sparser
"Pcsk9 mutations have been associated with autosomal dominant hypercholesterolemia, which is characterized by high LDL levels ( xref )."

sparser
"Population studies have shown that PCSK9 gain of function variants associate with high LDL-C levels and autosomal dominant hypercholesterolemia [ xref , xref ], whereas loss of function variants associate with low LDL-C levels [ xref , xref ]."
LimB affects ATXN3
| 2
| 2

sparser
"The 7q21.3-q22.1 region has been extensively studied because a locus determining an autosomal dominant form of a developmental limb abnormality, the split hand/split foot malformation ( SHFD1 ), was m[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Similarly, Adams-Oliver syndrome, a rare autosomal dominant disorder associated with terminal limb defects, may be caused by loss-of-function mutations in Notch1 and Dll4 as well as RBPJ ."
2 |
Hsa-miR-8485 decreases the amount of ATXN3. 2 / 2
2 |

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No evidence text available
Hsa-miR-603 affects ATXN3
2 |
Hsa-miR-603 decreases the amount of ATXN3. 2 / 2
2 |

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No evidence text available
2 |
Hsa-miR-5011-5p decreases the amount of ATXN3. 2 / 2
2 |

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No evidence text available
2 |
Hsa-miR-4789-5p decreases the amount of ATXN3. 2 / 2
2 |

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No evidence text available
2 |
Hsa-miR-4789-3p decreases the amount of ATXN3. 2 / 2
2 |

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No evidence text available
2 |
Hsa-miR-3941 decreases the amount of ATXN3. 2 / 2
2 |

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2 |
Hsa-miR-362-3p decreases the amount of ATXN3. 2 / 2
2 |

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2 |
Hsa-miR-329-3p decreases the amount of ATXN3. 2 / 2
2 |

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2 |
Hsa-miR-190a-3p decreases the amount of ATXN3. 2 / 2
2 |

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No evidence text available
2 |
Hsa-miR-1277-5p decreases the amount of ATXN3. 2 / 2
2 |

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Endocrine affects ATXN3
| 2
ATXN3 binds endocrine. 2 / 2
| 2

sparser
"Although non-endocrine tumors predominate in the DICER1 syndrome ( Figure 1 ), a multiple endocrine neoplasia phenotype is associated with this autosomal dominant familial cancer syndrome."

sparser
"Another autosomal dominant hamartomatous disorder associated with endocrine tumour development is Peutz-Jeghers syndrome (PJS)."
Dynein affects ATXN3
| 1
ATXN3 binds dynein. 1 / 2
| 1

reach
"In addition, ataxin-3 interacts with dynein and the cytosolic histone deacetylase 6 during transport of misfolded proteins in cultured cells, thus linking ataxin-3 to cytoskeletal transport processes."
CsnB affects ATXN3
| 2
| 2

sparser
"We report the results of molecular genetic analysis of an Irish family segregating an autosomal dominant form of CSNB in which a previously unreported threonine-to-isoleucine substitution at codon 94 in the rhodopsin gene was found to segregate with the disease."

sparser
"The clinical description, pedigree, dark adaptation and elctroretinogram (ERG) studies indicate that the patients have an autosomal dominant form (ad) of CSNB."
Crystallin affects ATXN3
| 2

sparser
"To date, many mutations in 12 human crystallin genes have been associated with inherited autosomal dominant (AD) and/or autosomal recessive (AR) cataract."

sparser
"Of these, mutations in 9 crystallin genes have been associated with autosomal dominant cataracts ( CRYAA , CRYAB , CRYBB1 , CRYBB2 , CRYBA1/A3 , CRYBA4 , CRYGC , CRYGD , and CRYGS ) but only 3 with autosomal recessive cataracts ( CRYAA , CRYBB1 , and CRYBB3 )."
Arginine affects VCP
| 2
VCP binds ATXN3 and arginine. 2 / 2
| 2

reach
"VCP binds ataxin-3 at an arginine rich region that precedes its polyQ portion (XREF_FIG)."

reach
"VCP is bound directly by ataxin-3 through an arginine rich area preceding the polyQ repeat."
2 |
Aflatoxin B1 increases the amount of ATXN3. 2 / 2
2 |

No evidence text available

No evidence text available
YUH1 affects ATXN3
| 2
| 2

sparser
"Although UCH is associated with MINDY and MJD based on the functional categories, the high number of catabolic functions makes it strictly different from the others."

sparser
"However, the papain fold is also seen in many non-DUB proteases and our HMM-to-HMM comparisons shown in xref and xref reveal significant sequence relationships only between some DUB and UbL-protease families such as ATX3-UCH or ZUFSP-UFSP-ATG4, leaving other families unconnected."
XPO1 affects ATXN3
| 1 1
| 1 1

reach
"In order to further exclude a possible direct interaction between CRM1 and ataxin-3, we took advantage of the observation that Ran in a complex with a transport receptor is resistant to the activity o[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In order to further exclude a possible direct interaction between CRM1 and ataxin-3, we took advantage of the observation that Ran in a complex with a transport receptor is resistant to the activity o[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
VCP affects arginine
| 2
VCP binds ATXN3 and arginine. 2 / 2
| 2

reach
"VCP binds ataxin-3 at an arginine rich region that precedes its polyQ portion (XREF_FIG)."

reach
"VCP is bound directly by ataxin-3 through an arginine rich area preceding the polyQ repeat."
USP13 affects ATXN3
1 |
1 |

No evidence text available
UBL affects PRKN
| 2
ATXN3 binds PRKN, Dnmt1, and UBL. 2 / 2
| 2

sparser
"For example, the interaction of the parkin Ubl domain with the UIM regions of the deubiquitinating protein ataxin-3 is required for the unique ubiquitin-editing role of ataxin-3 ( xref , xref )."

sparser
"The observation that the three UIM sites in ataxin-3 bind equally to a single site of the parkin Ubl domain suggests a multi- or polyvalent ligand binding mode."
UBE4B affects ATXN3
2 |
UBE4B ubiquitinates ATXN3. 2 / 2
2 |

"E4B interacted with, and thereby mediated polyubiquitylation of, ataxin-3"

"Furthermore, UFD2a, a mammalian ubiquitin-chain assembly factor (E4), associated with VCP and induced polyubiquitylation of MJD1"
UBE2L3 affects ATXN3
1 | 1
1 | 1

No evidence text available

sparser
"Parkin, its cognate E2 UbcH7, and Ataxin-3 form a tight complex preventing the autoubiquitination of Parkin and the release of UbcH7 [ xref ]."
TWSG1 affects ATXN3
| 2
| 2

sparser
"Cowden syndrome (CS), an autosomal dominant disease associated with mutations in the PTEN tumor suppressor gene on chromosome 10q10.22-23, is associated with elevated risks of breast and thyroid cancer, as well as diverse benign neoplasms including gastrointestinal hamartomatous polyps, lipomas, giant fibroadenomas of the breast, hemangiomas, and multiple early-onset uterine leiomyomas [ xref ]."

sparser
"Germline truncating mutation of the tumor suppressor gene is associated with an autosomal dominant colon cancer predisposition syndrome known as familial adenomatous polyposis coli, or FAP."
TWNK affects ATXN3
| 2
| 2

sparser
"The PEO1 ( C10ORF2 ) mutations are associated with autosomal dominant progressive external ophthalmoplegia (PEO) (PEOA3), parkinsonism, as well as infantileonset spinocerebellar ataxia. xref , xref , xref Biochemical analyses of purified mutant PEO1 proteins have been performed, but the results have been mixed. xref , xref , xref The functional defects of mutant Twinkle proteins have also been studied in the ortholog in Drosophila . xref Transgenic mice carrying a point mutation at amino acid residue 360 (A360T), or a short repeat of amino acids 353 to 365 reproduced histopathology and histochemical changes resulting from mitochondrial respiratory chain dysfunction."

sparser
"TWNK was also associated with autosomal dominant progressive external ophthalmoplegia (adPEO, OMIM #609286) which can result in ophthalmoparesis with exercise intolerance, ataxia, peripheral neuropathy and multiple mtDNA deletions and at least 40 causative mutations for adPEO have been identified up to now [ xref ]."
TTR affects ATXN3
| 2
| 2

sparser
"More than 80 TTR mutations have been associated with autosomal dominant amyloidosis, usually presenting with peripheral and autonomic neuropathy and/or cardiomyopathy."

sparser
"Subsequent sequencing of the patient's TTR gene identified a pathogenic variant that is associated with autosomal dominant hereditary transthyretin-mediated amyloidosis."
TPM3 affects ATXN3
| 2
| 2

sparser
"Using this approach we found that certain rhodopsin point mutants in TM1 and TM5 that are associated with autosomal dominant retinitis pigmentosa, reconstitute into vesicles as monomers but retain wild-type (WT)-like scramblase activity xref ."

sparser
"Autosomal dominant nemaline myopathy associated with a missense mutation in the TPM3 gene has been identified in one large family."
TOMM20L affects ATXN3
2 |
2 |

No evidence text available

No evidence text available
TLR2 affects ATXN3
| 1 1
TLR2 leads to the phosphorylation of ATXN3. 2 / 2
| 1 1

reach
"We show NOD2 and TLR2 mediate phosphorylation of the deubiquitinase ataxin-3 via RIPK2 and TBK1."

sparser
"Interestingly phosphorylation of ATAXIN3 by NOD2 and TLR2 in myeloid cells has been recently shown to mediate mitochondrial reactive oxygen species production and bacterial clearance xref ."
TFAP2B affects ATXN3
| 2
| 2

sparser
"Mutations in the gene encoding AP-2β are associated with Char syndrome, a human autosomal dominant disorder."

sparser
"Char syndrome is a rare autosomal dominant form of PDA with additional hand abnormalities and facial dysmorphisms caused by mutations in the TFAP2B gene ( xref , xref )."
TEX11 affects ATXN3
1 1 |
1 1 |

No evidence text available

No evidence text available
TCF4 affects ATXN3
| 1 1
| 1 1

reach
"Genes that were part of this signature on the brain side were the TCF4, ATN1, PPP2R2B, ATXN10 and ATXN3, which are associated with neurodegenerative and developmental disorders such as Pitt-Hopkins Syndrome [XREF_BIBR], Dentatorubral pallidoluysian atrophy [XREF_BIBR], SCA12 [XREF_BIBR], SCA10 [XREF_BIBR] and SCA3 [XREF_BIBR]."

sparser
"Heat-shock transcription factor 4 (HSF4) mutations are associated with autosomal dominant lamellar cataract and Marner cataract."
TARDBP affects ATXN3
| 2
| 2

sparser
"Autosomal dominant mutations in TDP-43 are associated with sporadic and familial ALS xref , xref , and the redistribution of TDP-43 from the nucleus to cytoplasm has been recognized as a pathological hallmark for most forms of ALS and most frequent subtypes of FTD xref , xref ."

sparser
"VCP appears to interact with Ataxin-3 and TDP-43 neuronal inclusions [31,32] , interacts with and promotes clearance of folding-deficient V2 vasopressin receptor mutants that cause X-linked nephrogen[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
STB affects ATXN3
| 2
| 2

reach
"STB/HAP1 can also interact with the causal agents of some other polyQ diseases, such as with Abelson helper integration site 1 in Joubert syndrome (Sheng et al., 2008), ataxin 3 in Machado-Joseph disease (Takeshita et al., 2011) and TATA binding protein in spinocerebellar ataxia type 17 (Prigge and Schmidt, 2007)."

reach
"The results clearly showed that HAP1 and STB interacts with the normal ataxin-3 through Josephin domain and polyglutamine expanded mutants derived from SCA3 as well."
SERPINH1 affects ATXN3
| 1 1
| 1 1

sparser
"Hence it seems unlikely that serpinH1 and ataxin-3 interaction plays an important functional role in SCA3, but may rather connect ataxin-3 localization to the ER."

reach
"Hence it seems unlikely that serpinH1 and ataxin-3 interaction plays an important functional role in SCA3, but may rather connect ataxin-3 localization to the ER."
S100A6 affects ATXN3
| 2
| 2

sparser
"There are several DNA tests currently available that are based on dominant or codominant mutations, including the RHO mutation for an autosomal dominant form of PRA in the English Mastiff offered by OptiGen () (Kijas et al. xref ), the mutation in HSF4 that is associated with hereditary cataract in the Australian Shepherd that is offered by the Animal Health Trust () (Mellersh et al. xref , xref ), and the polymorphisms associated with renal dysplasia in multiple breeds that is offered by DoGenes () (Whiteley et al. xref )."

sparser
"The only autosomal dominant form of PRA described so far has been reported in the Bull Mastiff and English Mastiff breeds."
RUNX1 affects ATXN3
| 2
| 2

sparser
"Deleterious mutations in RUNX1 (Runt-related transcription factor 1; MIM ID *151385) are associated with a rare Autosomal Dominant hereditary cancer syndrome (linked to 21q22.12) that is characterized by qualitative and quantitative platelet defects with a high-propensity for myelodysplastic syndrome (MDS), acute myeloid leukemia (AML), and lymphoid malignancies( xref )."

sparser
"In humans, heterozygous RUNX1 mutation is associated with an autosomal dominant disorder, the familial platelet disorder with predisposition to acute myeloid leukemia (FPD/AML) (Mendelian Inheritance in Man [MIM] 601399), characterized by impaired megakaryopoiesis, quantitative and qualitative defects in platelet function, and over 40% risk of development of myelodysplastic syndrome (MDS) or AML at a median age of 33 years [ xref – xref ]."
RRAGA affects ATXN3
| 2
| 2

sparser
"Herein, we report that mutations in the RagA GTPase ( RRAGA ), a key regulator of the mechanistic rapamycin complex 1 (mTORC1), are associated with autosomal dominant cataracts."

sparser
"Our findings thus indicate that the RRAGA mutations are associated with autosomal dominant cataracts through disruption of the mTORC1 pathway, a substantial modulator of ageing and age-related human diseases [ xref ]."
RNA-binding affects ATXN3
| 2
| 2

reach
"A set of studies with drosophila have shown that muscleblind (mbl), an RNA binding protein (implicated as a modifier in DM1), can enhance the toxicity of both Ataxin-3 protein and non coding CAG-repeat RNA [XREF_BIBR]."

reach
"Interestingly, muscleblind (mbl), an RNA binding protein implicated as a modifier in DM1, can dramatically enhance the toxicity of both the Ataxin-3 protein and non coding CAG-repeat RNA in Drosophila."
RAG2 affects ATXN3
| 2
RAG2 inhibits ATXN3. 2 / 2
| 2

reach
"UCHL1, UCHL3, USP2 and USP8 were found to be inhibited by AM146, RA-9, and RA-14, which did not inhibit Ataxin-3, A20, BAP1, Otubain 1 or USP7."

reach
"UCHL1, UCHL3, USP2 and USP8 were found to be inhibited by AM146, RA-9, and RA-14 which did not inhibit Ataxin-3, A20, BAP1, Otubain 1 or USP7 [XREF_BIBR]."
RAD23B affects RAD23A
| 2
| 2

sparser
"It has been reported that ATX3 interacts with HHR23A and HHR23B, homolog of DNA repair protein RAD23 ( xref ), and that ATX3 can be recruited to the DNA damage sites induced by laser microirradiation ( xref )."

sparser
"Initial studies found that ataxin-3 interacts with two proteins, HHR23A and HHR23B, that are both homologs of the DNA repair protein Rad23 [ xref ]."
PSMC5 affects ATXN3
| 2
PSMC5 inhibits ATXN3. 2 / 2
| 2

reach
"In addition, several groups demonstrated that inhibition of the proteasome in cell culture and mammalian cells results in increased aggregation and cytotoxicity in SCA 3 and HD [XREF_BIBR, XREF_BIBR], whereas an overexpression of p45 (ATPase of 19S subunit of proteasome) stimulates degradation of ataxin-3 [XREF_BIBR]."

reach
"P45, an ATPase subunit of the 19S proteasome, interacts with ataxin-3 in vitro and stimulates the degradation of ataxin-3 in an in vitro reconstituted degradation assay system."
PSEN1 affects ATXN3
| 2
| 2

sparser
"AD is sporadic (sAD) in the great majority of cases, but 1–5% of Alzheimer patients suffer from the autosomal dominant form of the disease (ADAD), which is caused by mutations in the presenilin 1 ( PSEN1 ), presenilin 2 ( PSEN2 ), or amyloid precursor protein ( APP ) genes xref ."

sparser
"Seizures and myoclonus is common feature of autosomal dominant EOAD, which was associated with PSEN1 mutation [ xref , xref ]."
PROM1 affects ATXN3
| 2
| 2

sparser
" PROM1 (OMIM 612657) codes for a transmembrane glycoprotein (Prominin-1) that localizes to the base of the rod and cone outer segments and is involved in disc assembly and maintenance of outer segment structure. xref PROM1 is associated with both autosomal dominant (AD) xref , xref and AR xref retinal dystrophies, with the AD forms tending to be later onset and milder. xref PROM1 is responsible for between 1.0% and 9.5% of AR CRD (arCRD) cases. xref , xref , xref "

sparser
"Missense mutations in the PROM1 gene have mostly been associated with autosomal dominant forms of Stargardt-like macular dystrophy, bull’s eye macular dystrophy, and cone-rod dystrophy."
| PMC
PRKN affects UBL
| 2
ATXN3 binds PRKN, Dnmt1, and UBL. 2 / 2
| 2

sparser
"For example, the interaction of the parkin Ubl domain with the UIM regions of the deubiquitinating protein ataxin-3 is required for the unique ubiquitin-editing role of ataxin-3 ( xref , xref )."

sparser
"The observation that the three UIM sites in ataxin-3 bind equally to a single site of the parkin Ubl domain suggests a multi- or polyvalent ligand binding mode."
POLR2A affects HTT
| 2
HTT binds ATXN3 and POLR2A. 2 / 2
| 2

sparser
"PNKP has been shown to promote transcription-coupled double-strand break repair ( xref ) and often performs transcription-coupled repair when in complex with huntingtin protein, RNA polymerase II subunit A, ataxin-3, and cyclic AMP-response element binding protein (CBP) ( xref )."

sparser
"The mechanisms for this were recently suggested to be through direct interactions between HTT and RNA polymerase II subunit A (POLR2A), ataxin-3, the DNA repair enzyme polynucleotide-kinase-3'-phosphatase (PNKP), and cyclic AMP-response element-binding (CREB) protein (CBP) [ xref ], as well as complexes with the DNA double-strand break repair complex Ku70/Ku80 [ xref ]."
PML affects ATXN3
2 |
2 |

No evidence text available

No evidence text available
PLD affects ATXN3
| 2
ATXN3 binds PLD. 2 / 2
| 2

sparser
"The most common form of PLD is associated to Autosomal Dominant Polycystic Kidney Disease (ADPKD), a genetic disease affecting more than six million people worldwide xref , xref ."

sparser
"Interestingly, PLD is usually associated with a renal anomaly called autosomal dominant polycystic kidney disease (ADPKD), suggesting a certain universal mechanism underlying tubular formation and/or function among different organs."
PKD1 affects ATXN3
| 2
| 2

sparser
"Hypertension and aneurysm are frequently associated with autosomal dominant polycystic kidney disease (ADPKD) caused by polycystin-1 (PC1) mutations, which is closely related to endothelial dysfunction."

sparser
"Mutation of a ciliary protein, polycystin-1 (PC1) is associated with autosomal dominant polycystic kidney disease."
PITX2 affects ATXN3
| 2
| 2

sparser
"Furthermore, the altered pitx2 expression pattern, together with the described morphological features of the lens-ablated fish suggests that Astyanax mexicanus could be considered as an alternative teleost model organism in which to study Axenfeld-Rieger syndrome (ARS), a rare autosomal dominant developmental disorder that is associated with PITX2 and which has both ocular and non-ocular abnormalities."

sparser
"Several studies have reported that mutation of PITX2 is associated with the pathogenesis of Axenfeld-Rieger syndrome (ARS) which is an autosomal dominant human disease characterized by developmental defects of eye, teeth and heart xref ."
PICK1 affects ATXN3
1 1 |
1 1 |

No evidence text available

No evidence text available
PHEX affects FGF23
| 2
| 2

sparser
"The list of the diseases associated with OPLL includes hypophosphatemic rickets/osteomalacia, including an autosomal dominant form (MIM 193100) caused by FGF23 mutations, an X-linked dominant form (MIM 307800) caused by PHEX mutations, an X-linked recessive form (MIM 300554) caused by CLCN5 mutations, and autosomal recessive forms caused by DMP1 (MIM 600980) and ENPP1 (MIM 173335) mutations."

sparser
"Several forms of hypophosphatemic rickets are known, including an X-linked form (MIM 307800) caused by phosphate regulating endopeptidase homolog, X-linked ( PHEX ) mutations (MIM 300550), an autosomal dominant form (MIM 193100) caused by fibroblast growth factor 23 ( FGF23 ) mutations (MIM 605380), an X-linked recessive form (MIM 300554) caused by chloride channel, voltage-sensitive 5 ( CLCN5 ) mutations (MIM 300008), and autosomal recessive forms caused by dentin matrix acidic phosphoprotein 1 ( DMP1 ) (MIM 600980), hypophosphatemic rickets, autosomal recessive 2 ( ARHR2 ) (MIM 613312) or ectonucleotide pyrophosphatase/phosphodiesterase 1 ( ENPP1 ) (MIM 173335) mutations. 'tiptoe walking' ( TTW ) mouse, which has a spontaneous nonsense mutation in ENPP1 is a good model for OPLL.[ xref ] Also, OPPL is a frequent complication in patients with endocrine disorders including hypoparathyroidism[ xref ] and acromegaly/gigantism.[ xref ] However, most cases of OPLL are idiopathic."
PHA affects ATXN3
| 2
| 2

sparser
"TM The autosomal dominant form of PHA presents with an asymptomatic to moderate clinical picture and mineralocorticoid resistance restricted only to the kid- ney."

sparser
"This is an autosomal dominant form of PHA1."

sparser
"Pcsk9 mutations have been associated with autosomal dominant hypercholesterolemia, which is characterized by high LDL levels ( xref )."

sparser
"Population studies have shown that PCSK9 gain of function variants associate with high LDL-C levels and autosomal dominant hypercholesterolemia [ xref , xref ], whereas loss of function variants associate with low LDL-C levels [ xref , xref ]."
NOS2 affects ATXN3
| 2
| 2

reach
"We thus sought to determine whether ataxin-3 interacted with the CHIP substrate GFP INOS."

reach
"In lysates from transfected cells, immunoprecipitation of ataxin-3 confirmed that ataxin-3 interacts with GFP INOS in cells (XREF_FIG)."
NOD2 affects ATXN3
| 1 1
NOD2 leads to the phosphorylation of ATXN3. 2 / 2
| 1 1

sparser
"Interestingly phosphorylation of ATAXIN3 by NOD2 and TLR2 in myeloid cells has been recently shown to mediate mitochondrial reactive oxygen species production and bacterial clearance xref ."

reach
"We show NOD2 and TLR2 mediate phosphorylation of the deubiquitinase ataxin-3 via RIPK2 and TBK1."

reach
"On the other hand, the observation that MG132 blocked SUMO induced degradation of ataxin-3, although the effect was not strong, suggested a role of UPS for this process."

reach
"Azure C or MG132 that inhibit HSP70 function or proteasome activity can also significantly increase the protein level of Atx3 71Q (XREF_FIG), as in the case of Atx3 22Q (XREF_FIG & XREF_FIG)."

reach
"Defects in ataxin-3 proteins and CAG repeat expansions in its coding gene ATXN3 cause Spinocerebellar Ataxia Type 3 (SCA3) or Machado-Joseph disease (MJD) polyglutamine neurodegenerative disease."

reach
"Mutations in ataxin-3 cause spinocerebellar ataxia type 3 (SCA3), a neurodegenerative disorder that is a member of the polyglutamine family of diseases."
MUC1 affects ATXN3
| 2
| 2

sparser
"Certain hereditary kidney diseases cannot be diagnosed by using exome sequencing (eg, MUC1-autosomal dominant tubulointerstitial kidney disease)."

sparser
"For example, causal variants in the MUC1 gene, which is associated with autosomal dominant tubulointerstitial kidney disease (ADTKD- MUC1 ; OMIM 174000) and contains a highly repetitive, GC-rich sequence, were missed by NGS-based regional capture, WES, and WGS and were identified only by long-range PCR and molecular cloning xref ."
MID1 affects CSA
| 2
| 2

sparser
"In this study we show that MID1 can also bind to the CAG repeat region of ATXN2, ATXN3 , and ATXN7 in vitro , suggesting that binding of MID1 to CAG repeats is not dependent on the flanking regions."

sparser
"We show that ATXN2, ATXN3 , and ATXN7 bind to MID1 in a CAG repeat length-dependent manner."
MGRN1 affects ATXN3
2 |
2 |

No evidence text available

No evidence text available
MED12 affects ATXN3
| 2
| 2

sparser
"In two large Italian families with autosomal dominant OPA1 mutations, adCPEO was associated with parkinsonism in several patients, whereas symptomatic optic atrophy was absent in most of the affected individuals (Carelli et al xref )."

sparser
"For example in the case of OPA1, the autosomal dominant mutations are associated with reduced OPA1 expression (Bonifert et al , xref ), while for the recessive form almost absent protein expression was found (Spiegel et al , xref )."
MARCHF5 affects ATXN3
1 | 1
MARCHF5 ubiquitinates ATXN3. 2 / 2
1 | 1

reach
"For instance, both mutant SOD1, a misfolded mitochondrial protein associated with the onset of amyotrophic lateral sclerosis, and polyglutamine expanded ataxin-3, a pathogenic protein causing Machado-Joseph disease, are ubiquitinated by MITOL and then degraded by the proteasome [XREF_BIBR - XREF_BIBR]."
MAPT affects ATXN3
| 2
| 2

sparser
"Initial translational efforts might, therefore, be more successful in disorders with more homogeneous underlying pathology, such as the primary tauopathies, progressive supranuclear palsy (PSP) and autosomal dominant frontotemporal lobar degeneration (FTLD) associated with MAPT mutations, in all of which tau is the major abnormal protein."

sparser
"The potential role of tau in various frontotemporal dementias has been further strengthened with recent reports of several autosomal dominant hereditary forms of frontotemporal dementia (sometimes ass[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
LMNA affects ATXN3
| 2
| 2

sparser
"Asp300Gly) in the LMNA protein has been associated with an autosomal dominant late-onset cardiocutaneous progeria [ xref ]."

sparser
"FPLD (MIM 151660) is a rare autosomal dominant form of insulin resistance caused by mutant LMNA, which encodes nuclear lamin A/C, a structural component of the nuclear envelope.[ xref ] FPLD is characterized by loss of fat affecting the limbs and trunk, accumulation of fat in the neck and face, and predisposition to insulin resistance, leading to complications such as glucose intolerance, dyslipidemia, high blood pressure, liver steatosis, and increased risk for coronary heart disease."
LIPC affects ATXN3
| 2
| 2

sparser
"In autosomal dominant forms of syndromic HL which tend to have a variable expression, a key feature for the diagnosis may be found in a relative rather than in the proband."

sparser
"DFNA5 was originally identified as a gene responsible for an autosomal dominant form of HL in a Dutch family (Van Laer et al., xref )."
LC3C affects ATXN3
| 2
ATXN3 binds LC3C. 2 / 2
| 2

reach
"Moreover, Ataxin-3 interacts with the autophagosomal membrane proteins LC3C and GABARAP and transiently localized to early autophagosomes."

reach
"The Machado-Joseph disease deubiquitylase ataxin-3 interacts with LC3C and GABARAP and promotes autophagy."
KLF4 affects ATXN3
| 1 1
| 1 1

reach
"Subsequent immunoprecipitation assays confirmed that ATXN3 bound to KLF4, mediating the deubiquitination and stabilization of KLF4 protein levels."

sparser
"Subsequent immunoprecipitation assays confirmed that ATXN3 bound to KLF4, mediating the deubiquitination and stabilization of KLF4 protein levels."
KIT affects ATXN3
| 2
| 2

sparser
"In humans, c-kit mutation is associated with an autosomal dominant disorder of melanocyte development, piebaldism, which is characterized by leukoderma and poliosis."

sparser
"However, germline autosomal dominant mutations of KIT have been associated with familial multiple GISTs in paediatric patients [ xref ]."
JAG1 affects ATXN3
| 2
| 2

sparser
"Mutations in the human homologue of Jag1 cause Alagille syndrome (AGS), an autosomal dominant disorder associated with liver, heart, eye and skeletal abnormalities, accompanied by a characteristic facies."

sparser
"Also, various mutations of the Notch ligand JAGGED1 have been associated with the autosomal dominant Alagille syndrome in which misshaped vertebrae (butterfly vertebrae) are observed (AGS [MIM 118450 ]) ( xref ; xref )."
Integrins affects ATXN3
| 1 1
| 1 1

sparser
"Ataxin-3 interacts with α5 integrin subunit and represses its degradation."

reach
"Because ATXN3 interacts with and stabilizes alpha5 integrin in a DUB activity dependent manner, it most likely regulates the degradation of this protein through its role in ubiquitin dependent proteostasis."
IMMT affects ATXN3
| 1 1
| 1 1

sparser
"In myeloid cells ataxin-3 associates with the mitochondrial cristae protein MIC60, and is required for oxidative phosphorylation."

reach
"In myeloid cells ataxin-3 associates with the mitochondrial cristae protein MIC60, and is required for oxidative phosphorylation."
IGHD affects ATXN3
| 2
| 2

sparser
"These data indicate that the R183H mutant GH, although causing an autosomal dominant form of IGHD has an identical effect on GHR/GHBP transcription as its wild-type, the 22-kDa GH."

sparser
"In total, we identified pathogenic GH1 defects in 9 of 38 patients with IGHD (23%), eight with the autosomal recessive and one with the autosomal dominant inheritance form of IGHD."
Histone affects ATXN3
| 1 1
| 1 1

sparser
"Interactions of ataxin-3 and other regulators of histone acetylation and transcription (p300, CREB-binding protein) have been detected ( xref )."

reach
"Although the exact cellular roles of ataxin 1 and 3 are not well understood yet, both seem to have nuclear functions: Ataxin1 is a chromatin binding factor and ataxin 3 binds to histones and regulates transcription."
Heat affects ATXN3
| 2
Heat activates ATXN3. 2 / 2
| 2

reach
"Heat shock, a general proteotoxic stress, also induced wild-type and pathogenic Atx3 to accumulate in the nucleus."

reach
"Heat shock or oxidative stress leads ATXN3 to accumulate in the nucleus."
HTT affects POLR2A
| 2
HTT binds ATXN3 and POLR2A. 2 / 2
| 2

sparser
"PNKP has been shown to promote transcription-coupled double-strand break repair ( xref ) and often performs transcription-coupled repair when in complex with huntingtin protein, RNA polymerase II subunit A, ataxin-3, and cyclic AMP-response element binding protein (CBP) ( xref )."

sparser
"The mechanisms for this were recently suggested to be through direct interactions between HTT and RNA polymerase II subunit A (POLR2A), ataxin-3, the DNA repair enzyme polynucleotide-kinase-3'-phosphatase (PNKP), and cyclic AMP-response element-binding (CREB) protein (CBP) [ xref ], as well as complexes with the DNA double-strand break repair complex Ku70/Ku80 [ xref ]."
HSF4 affects ATXN3
| 2
| 2

sparser
"Hsf4 mutations are associated with autosomal dominant lamellar and Marner cataract [ xref ]."

sparser
"In humans, mutations in HSF4 have been associated with both autosomal dominant and recessive cataracts."
HNF1A affects ATXN3
| 2
| 2

sparser
"Heterozygous germline mutations in HNF1A are responsible for an autosomal dominant form of diabetes MODY3 (maturity onset diabetes of the young type 3)."

sparser
"Mutations in the HNF1alpha gene are associated with an autosomal dominant form of non-insulin-dependent diabetes mellitus called maturity-onset diabetes of the young (MODY3)."
HHR23 affects ATXN3
| 2
ATXN3 binds HHR23. 2 / 2
| 2

reach
"Ataxin-3 also associates with HHR23, the UPP-escort protein VCP and p97, UBXN-5 protein and with the proteasome, suggesting a role in the modulation of protein degradation XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR."

reach
"In addition to the 26S proteasome and ubiquitylated substrate, HHR23 also interacts with ataxin-3 in vitro, a protein involved in the development of the neurodegenerative Machado-Joseph disease [XREF_BIBR]."
HGF affects ATXN3
| 2
| 2

sparser
"Linkage analysis of HGF patients revealed several chromosomal regions that may contain mutations responsible for isolated, non-syndromic autosomal dominant forms of HGF."

sparser
"Loci for isolated, non-syndromic autosomal dominant forms of HGF have been localized to chromosome 2p21-p22 in a Brazilian family [ xref ], and to chromosome 5q13-q22 [ xref ] and 11p15 in a number of Chinese families [ xref ]."
Gln-Gln affects ATXN3
| 2
| 2

reach
"We noticed that more atx3 was bound to the p97 QQ than to wt p97 (XREF_FIG, lane 4 vs. 2; XREF_FIG)."

reach
"The expression of atx3 reduced the amount of ubiquitinated proteins associated with wt p97 (XREF_FIG, lane 2 vs. lane 1), but not of those bound by p97 QQ (lane 4 vs. lane 3), although more atx3 was bound by p97 QQ (lane 4 vs. lane 2)."
GYS affects ATXN3
| 1 1
GYS phosphorylates ATXN3 on S256. 2 / 2
| 1 1

reach
"Phosphorylation of ataxin-3 by glycogen synthase kinase 3beta at serine 256 regulates the aggregation of ataxin-3."

sparser
"A study demonstrated that glycogen synthase kinase 3β (GSK 3β)-induced phosphorylation of Ataxin3 at Ser256 can regulate the aggregation of Ataxin3 ( xref )."
GST affects ATXN3
| 2
| 2

sparser
"In contrast, a p97 mutant lacking its N-terminal domain (N-domain) failed to bind GST-atx3 ( xref )."

sparser
"Intriguingly, the results of a GST pulldown assay with purified GST-ATXN3 revealed that ATXN3 interacted with both His-TSPAN8 and His-PTCH1 (Supplementary Fig.  xref )."
GJB3 affects ATXN3
| 2
| 2

sparser
"In addition, heterozygous mutations in the GJB3 and KCNQ have been associated with autosomal dominant hearing loss [ xref , xref ]."

sparser
"Mutations in (OMIM: ) were originally shown to underlie an autosomal dominant form of non-syndromic deafness DFNA2 (OMIM:) in Chinese patients and the c."
GFI1B affects ATXN3
| 2
| 2

sparser
"We now demonstrate that both autosomal dominant and autosomal recessive αδ-SPD are associated with mutations in GFI1B ."

sparser
"We now demonstrate that both autosomal dominant and autosomal recessive αδ-SPD are associated with mutations in GFI1B . Patients were enrolled in clinical protocol 76-HG-0238, “Diagnosis and treatmen[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
GCH1 affects ATXN3
| 2
| 2

sparser
"Mutations in the GCH1 gene are associated with levodopa responsive dystonia (DRD, also known as DYT5 dystonia), an autosomal dominant neurological movement disorder ( xref ; xref ; xref ; xref )."

sparser
"Finally, mutations causing the autosomal dominant form of dopa-responsive dystonia have been identified in the gene coding for GTP cyclohydrolase I. Mutations in tyrosine hydroxylase have been identified in two brothers and put forward as evidence of an autosomal recessive form of the disease."
FOXO affects ATXN3
| 1 1
| 1 1

sparser
"Moreover, interactions of SCA3 with Foxo mediated by coiled-coil domains of these two proteins resulted in functional impairment of this transcription factor, whereas its overexpression significantly rescued the SCA3-induced defects."

reach
"ATXN3 interacts with and stabilizes the FOXO transcription factor FOXO4, and upon oxidative stress, they both translocate to the nucleus and activate manganese superoxide dismutase (SOD2) transcription, which in turn protects cells from oxidative damage."
FH affects ATXN3
| 2
| 2

sparser
"Autosomal dominant mutations in FH are associated with hereditary leiomyomatosis and renal cell cancer, indicating that FH functions as a tumor suppressor ( xref ; xref )."

sparser
"HLRCC-associated RCC occurs in patients with HLRCC syndrome, which is an autosomal dominant hereditary disease that is associated with germline mutations in FH at chromosome 1q42.3-q43."
FGFR1 affects ATXN3
| 2
| 2

sparser
"Recently, loss-of-function mutations of fibroblast growth factor receptor-1 (FGFR1) were associated with an autosomal dominant form of Kallmann syndrome."

sparser
"Other different modes of KS transmission (autosomal dominant and recessive) have since been described [ xref ], among them the autosomal dominant form caused by mutation of the gene FGFR1 , initially defined as KAL2 gene [ xref ]."
FGF23 affects PHEX
| 2
| 2

sparser
"The list of the diseases associated with OPLL includes hypophosphatemic rickets/osteomalacia, including an autosomal dominant form (MIM 193100) caused by FGF23 mutations, an X-linked dominant form (MIM 307800) caused by PHEX mutations, an X-linked recessive form (MIM 300554) caused by CLCN5 mutations, and autosomal recessive forms caused by DMP1 (MIM 600980) and ENPP1 (MIM 173335) mutations."

sparser
"Several forms of hypophosphatemic rickets are known, including an X-linked form (MIM 307800) caused by phosphate regulating endopeptidase homolog, X-linked ( PHEX ) mutations (MIM 300550), an autosomal dominant form (MIM 193100) caused by fibroblast growth factor 23 ( FGF23 ) mutations (MIM 605380), an X-linked recessive form (MIM 300554) caused by chloride channel, voltage-sensitive 5 ( CLCN5 ) mutations (MIM 300008), and autosomal recessive forms caused by dentin matrix acidic phosphoprotein 1 ( DMP1 ) (MIM 600980), hypophosphatemic rickets, autosomal recessive 2 ( ARHR2 ) (MIM 613312) or ectonucleotide pyrophosphatase/phosphodiesterase 1 ( ENPP1 ) (MIM 173335) mutations. 'tiptoe walking' ( TTW ) mouse, which has a spontaneous nonsense mutation in ENPP1 is a good model for OPLL.[ xref ] Also, OPPL is a frequent complication in patients with endocrine disorders including hypoparathyroidism[ xref ] and acromegaly/gigantism.[ xref ] However, most cases of OPLL are idiopathic."
EWSR1 affects ATXN3
1 1 |
1 1 |

No evidence text available

No evidence text available
EFNA3 affects ATXN3
| 2
EFNA3 increases the amount of ATXN3. 2 / 2
| 2

reach
"These results indicate that ATXN3 represses Efna3 transcription by acting, either directly or indirectly, on the promoter region."

reach
"Both normal and expanded ATXN3 significantly repressed Efna3 transcription by approximately 20% but had no effect on expression of the control pGL3 luciferase vector (XREF_FIG)."
Dnmt1 affects UBL
| 2
ATXN3 binds PRKN, Dnmt1, and UBL. 2 / 2
| 2

sparser
"For example, the interaction of the parkin Ubl domain with the UIM regions of the deubiquitinating protein ataxin-3 is required for the unique ubiquitin-editing role of ataxin-3 ( xref , xref )."

sparser
"The observation that the three UIM sites in ataxin-3 bind equally to a single site of the parkin Ubl domain suggests a multi- or polyvalent ligand binding mode."
DSBs affects ATXN3
| 2
ATXN3 binds DSBs. 2 / 2
| 2

reach
"An alternative explanation for the recruitment of ataxin-3 to DSBs is the presence of ubiquitylated chromatin, which could be recognized by the three UIMs present in ataxin-3."

reach
"The recruitment of ataxin-3 to DSBs is consistent with a recently reported systematic characterization of DUBs, which showed that ataxin-3 accumulates at laser inflicted DNA damage (Nishi etal, 2014)."
DND1 affects ATXN3
2 |
2 |

No evidence text available

No evidence text available
DICER1 affects ATXN3
| 2
| 2

sparser
"Germline mutations in DICER1 are associated with a distinct autosomal dominant tumor predisposition syndrome (OMIM #601200), whereby patients have an increased risk of developing a variety of extremely rare tumors, such as pleuropulmonary blastoma and PBs."

sparser
"Germline mutations in DICER1 are associated with an autosomal dominant hereditary cancer predisposition syndrome that confers an increased risk for the development of several rare childhood and adult-onset tumors, the most frequent of which include pleuropulmonary blastoma, ovarian sex cord-stromal tumors, cystic nephroma, and thyroid gland neoplasia."
Calpeptin affects ATXN3
| 2
| 2

reach
"Whilst it may be questioned whether the treatment with BLD-2736 may have instead decreased full-length ataxin-3 through effects on the expression of EGFP-ataxin-3 via an effect on transcription, we previously demonstrated that treatment with calpeptin decreased the presence of full length human ataxin-3 protein, without altering the levels of human ataxin-3 mRNA, and that this effect was prevented by cotreatment with the autophagy inhibitor, chloroquine [43]."
| PMC

reach
"In vitro experiments revealed that calpeptin treatment was able to prevent generation of ataxin-3 cleavage fragments and aggregates in iPSC-derived SCA3 neurons, proving the validity of this approach [49]."
CYP74A affects ATXN3
| 2
| 2

sparser
"Recently, mutations in ARHGAP31 and RBPJ have been found causing autosomal dominant forms of AOS."

sparser
"Autosomal dominant forms of AOS are linked to mutations in ARHGAP31, DLL4, NOTCH1 or RBPJ, while DOCK6 and EOGT underlie autosomal recessive inheritance."
CSA affects MID1
| 2
| 2

sparser
"In this study we show that MID1 can also bind to the CAG repeat region of ATXN2, ATXN3 , and ATXN7 in vitro , suggesting that binding of MID1 to CAG repeats is not dependent on the flanking regions."

sparser
"We show that ATXN2, ATXN3 , and ATXN7 bind to MID1 in a CAG repeat length-dependent manner."
CRX affects ATXN3
| 2
| 2

sparser
"This large deletion of the CRX gene is associated with a severe form of autosomal dominant retinal degeneration."

sparser
"Mutations in human CRX (NCBI Reference Sequence: NG_008605.1) have been associated with autosomal dominant forms of the retinal degenerative diseases Retinitis Pigmentosa (adRP), Cone-Rod Dystrophy (adCoRD) and Leber Congenital Amaurosis (adLCA), with different ages of onset and severity xref xref – xref ."
CREB1 affects ATXN3
2 |
2 |

No evidence text available

No evidence text available
CRC affects ATXN3
| 2
| 2

sparser
"Two major autosomal dominant forms of heritable CRC are familial adenomatous polyposis (FAP) and Lynch syndrome (also known as hereditary nonpolyposis colorectal cancer)."

sparser
"Lynch syndrome (LS) or hereditary non-polyposis colorectal cancer, is an autosomal dominant form of CRC that may account for up to 5% of all CRC cases. xref The penetrance for development of malignancy is up to 68.7% in males and 52% in females. xref Founder effects for LS have been discovered in several populations, including Newfoundland, Canada. xref It is believed that the increased rates of CRC in this province, which are among the highest in the world, may be attributable to a high rate of familial cancer in the population and possibly to the presence of novel susceptibility genes. xref , xref Familial adenomatous polyposis is another autosomal dominant inherited syndrome that greatly increases the risk of CRC and founder effects have been established. xref , xref , xref "
CK2beta affects ATXN3
| 2
ATXN3 binds CK2beta. 2 / 2
| 2

reach
"(2) In 293 cells, both wild type and expanded ataxin-3 interacted with CK2beta, but not CK2alpha."

reach
"Results (1) Both wild type and expanded ataxin-3 interacted with CK2alpha and CK2beta in vitro."
CK2alpha affects ATXN3
| 2
ATXN3 binds CK2alpha. 2 / 2
| 2

sparser
"Results (1) Both wild type and expanded ataxin-3 interacted with CK2alpha and CK2beta in vitro. (2) In 293 cells, both wild type and expanded ataxin-3 interacted with CK2beta, but not CK2alpha. (3) CK2 phosphorylated wild type and expanded ataxin-3."

sparser
"ATXN3 associated with CK2alpha and pharmacological inhibition of CK2 decreased nuclear ATXN3 levels and the formation of nuclear inclusions."
CHMP4B affects ATXN3
| 2
| 2

sparser
"Interestingly, a CHMP4B mutation associated with autosomal dominant posterior polar cataract abolishes the ability of CHMP4B to localize to micronuclei."

sparser
"As a CHMP4B mutation associated with an autosomal dominant form of cataract abolishes the ability of CHMP4B to localize to micronuclei, the autophagic degradation of DNA is implicated in the protection of lens cells from cataract development."
CHI3L1 affects ATXN3
| 2
| 2

sparser
"Using EM and AFM, we show that at 37°C ataxin-3 forms oligomers with diameters of ∼7–11 nm, which seem to further assemble into filaments."

sparser
"55 Non-pathological recombinant ataxin-3 forms oligomers in vitro , displaying a pathogenic conformation, under physiological conditions."
CDK5RAP2 affects ATXN3
| 2

sparser
"Three of the 12 were compatible with the autosomal dominant form of microcephaly with chorioretinopathy (MIM 156590), possibly as a new mutation."

sparser
"This appears to be a first report of a autosomal dominant form of microcephaly associated with mild to moderate mental retardation in contrast to absent or mild mental retardation described in earlier reports."
CDK5 affects ATXN3
| 2
CDK5 inhibits ATXN3. 2 / 2
| 2

reach
"CDK5 inhibition also increased aggregation of ataxin-3 (Liman et al., 2014)."

reach
"Moreover, reduction of CDK5 expression levels by RNAi in vivo enhances SCA3 toxicity as assayed in a Drosophila model for SCA3."
CAST affects ATXN3
| 2
CAST increases the amount of ATXN3. 2 / 2
| 2

reach
"Calpeptin (a calpain inhibitor) decreased levels of atxn3 cleaved fragments in atxn3-84Q zebrafish and rescued the motor phenotype, but it also removed all ATXN3 expanded protein due to an increase in autophagic flux (indicated by reduced p62 levels and increased LC3II levels) that cleared autolysosomes."
| PMC

reach
"Oral administration of the calpain inhibitor BDA-410 decreased both fragments formation and full-length ataxin-3 levels, reduced aggregation of mutant ataxin-3 and prevented cell injury and striatal and cerebellar degeneration."
CASR affects ATXN3
| 2
| 2

sparser
"CaSR abnormalities are associated with three hypocalcemic disorders, which are autosomal dominant hypocalcemia (ADH), Bartter syndrome type V and autoimmune hypoparathyroidism ()."

sparser
"However, it may be that gain-of-function CaSR mutations, which cause autosomal dominant hypocalcemia (ADH) ( xref ), are associated with abnormalities of glucose homeostasis and not FHH-associated loss-of-function CaSR mutations."
CAPN5 affects ATXN3
| 2
| 2

sparser
"CAPN5 has been associated with autosomal dominant neovascular inflammatory vitreoretinopathy (ADNIV) [ xref – xref ], obesity [ xref ], Huntington’s disease [ xref , xref ], and polycystic ovary syndrome [ xref ]."

sparser
"Mutations in CAPN5 are associated with the devastating retinal degenerative disease autosomal dominant neovascular inflammatory vitreoretinopathy (ADNIV). xref xref – xref ADNIV is a hereditary autoimmune disease of the eye that is characterized by abnormal retinal pigmentation, retinal neovascularization, photoreceptor degeneration, vitreous hemorrhage, intraocular fibrosis, and tractional retinal detachment."
C9orf72 affects ATXN3
| 1 1
| 1 1

reach
"Further studies, particularly on the interaction between C9orf72 and ataxin-3, are needed to verify our results."

sparser
"Further studies, particularly on the interaction between C9orf72 and ataxin-3, are needed to verify our results."
BSCL2 affects ATXN3
| 2
| 2

sparser
"ERPO may represent an ER quality control pathway for multiple ER substrates, since aggregation-prone mutant forms of seipin that are associated with an autosomal dominant motor neuron disease, accumulate in the same compartment [ xref ]."

sparser
"Mutant VAPB did not codistribute with mutant forms of seipin that are associated with an autosomal dominant motor neuron disease, and accumulate in a protective ER derived compartment termed ERPO (ER protective organelle) in neurons."
BEST1 affects ATXN3
| 2
| 2

sparser
"Best vitelliform macular dystrophy (BVMD) is autosomal dominant juvenile onset maculopathy, which is associated with a mutation in human BEST1 gene.[ xref ] hBEST1 gene encodes bestrophin-1 (Best1) protein, which is expressed basolaterally in retinal pigment epithelium (RPE) [ xref ] and in astrocytes.[ xref ] BVMD involves several stages and leads to loss of central vision."

sparser
"BEST1 is associated with autosomal dominant vitreoretinochoroidopathy, vitelliform macular dystrophy, and autosomal-recessive bestrophinopathy xref ."
| PMC
BAG3 affects ATXN3
1 | 1
1 | 1

No evidence text available

sparser
"Bag3 mutations in humans are associated with autosomal dominant forms of myofibrillar myopathy and dilated cardiomyopathy (Selcen et al., xref ; Norton et al., xref )."
Atx7 affects ATXN3
| 2
Atx7 activates ATXN3. 2 / 2
| 2

reach
"However, blocking of the autophagic degradation exhibited no significant influence on the decline of Atx3 caused by Atx7 93Q -N172 or Htt 100Q -N90 (Supplemental Fig. 2B, D)."

reach
"As shown in the result, expression of Atx7 93Q -N172 caused a remarkable decline of soluble Atx3 in the supernatant fraction; whereas it increased the insoluble aggregates appeared in the pellet."
| 2

sparser
"Familial early-onset AD (FAD) is associated with autosomal dominant mutations in the amyloid precursor protein (APP) and in the catalytic subunits (presenilin 1 and presenilin 2) of the intramembrane protease that processes it, γ-secretase ( xref )."

sparser
"Early-onset autosomal dominant AD genes are associated with excessive accumulation, however cognitive impairment best correlates with NFTs and disrupted microtubules."
ATXN7 affects CSA
| 2
| 2

sparser
"In this study we show that MID1 can also bind to the CAG repeat region of ATXN2, ATXN3 , and ATXN7 in vitro , suggesting that binding of MID1 to CAG repeats is not dependent on the flanking regions."

sparser
"We show that ATXN2, ATXN3 , and ATXN7 bind to MID1 in a CAG repeat length-dependent manner."

reach
"Because atx3 binds the ubiquitin like domain of rad23, which also mediates the interaction between rad23 and the proteasome, it is likely that rad23 may not interact with atx3 and the proteasome simultaneously."

reach
"Moreover, ataxin-3 contains two ubiquitin interacting motifs [16] and binds to the ubiquitin like domain in the DNA repair proteins HHR23A and HHR23B [17]."

sparser
"Ataxin-3 normally interacts with numerous transcriptional regulators including the forkhead box O (FOXO)-4 transcription factor, TATA-binding protein-associated factor TAFII130 [ xref ], CBP [ xref ], nuclear co-repressor receptor NCoR [ xref ], histone deacetylases [ xref ], and DNA repair protein RAD23 [ xref ]."

sparser
"These observations suggest that next to ubiquitin-proteasomal regulation of transcription, endogenous ataxin-3 might also directly interact with important transcriptional regulators."
ATXN3 affects superoxide
| 2
ATXN3 increases the amount of superoxide. 2 / 2
| 2

reach
"Ataxin-3 has been shown to activate transcription factor forkhead box protein O4 (FOXO4) and induce the expression of manganese superoxide dismutase (SOD2), an antioxidant enzyme, under oxidative stress conditions; however, when ataxin-3 is expanded, this transcriptional activation effect is reduced and, in fact, SOD2 levels are decreased in SCA3 patients brain samples ."

reach
"ATXN3 interacts with and stabilizes the forkhead box O (FOXO) transcription factor FOXO4, and upon oxidative stress they both translocate to the nucleus and activate manganese superoxide dismutase (SOD2) transcription which in turn protects cells from oxidative damage."
ATXN3 affects sub
| 2
ATXN3 activates sub. 2 / 2
| 2

reach
"The ubiquitination of ATXN3, primarily at site K117, activates the DUB function of ATXN3 through a conformational switch, which improves the editing of Ub chains on substrates."

reach
"Though normal and expanded ATXN3 bind similarly to polyubiquitinated parkin, pathogenic ATXN3 shows increased DUB activity towards polyUb-parkin, promoting its degradation via autophagy."
ATXN3 affects rpoB
| 2
| 2

sparser
"The autosomal dominant form of STL is caused by mutations in COL2A1 [ xref ], COL11A1 [ xref ], or COL11A2 (Gene ID 1302, OMIM) [ xref ], while the autosomal recessive form of STL is caused by mutations in COL9A1 (Gene ID 12839, OMIM) [ xref ], COL9A2 (Gene ID 1298, OMIM) [ xref ], COL9A3 (Gene ID 1299, OMIM) [ xref ], or LOXL3 (Gene ID 84695, OMIM) [ xref , xref ]."

sparser
"Autosomal dominant forms of STL include type I (membranous vitreous) [STL1] caused by mutation in the COL2A1 gene, type II (beaded vitreous form by COL11A1 mutation), and type III (nonocular by COL11A2 mutation)."
ATXN3 affects ribE
| 2
| 2

sparser
"Mutations in the ROR2 gene cause autosomal recessive RS (RRS) whereas mutations in WNT5A are responsible for the autosomal dominant (AD) form of RS."

sparser
"Mutations in WNT5A, locus 3p14.3 (OMIM 164975) are responsible for the autosomal dominant (AD) form of RS In these patients, oral manifestations are more prominent, hemivertebrae and scoliosis rarely occur and facial abnormalities tend to be milder ( xref )."
ATXN3 affects protein degradation
| 2
ATXN3 inhibits protein degradation. 2 / 2
| 2

sparser
"Moreover, since both wild type ataxin-3 and the catalytic inactive ataxin-3 mutant can inhibit ERAD, the ERAD inhibition phenotype associated with overexpression of wild type ataxin-3 likely results from a dominant negative effect rather than a gain in ataxin-3 function."

sparser
"Accordingly, inhibition of ERAD by ataxin-3 may allow more proteins to fold in the ER, and thus boosts the secretory capacity."
ATXN3 affects polyQ78
| 2
ATXN3 inhibits polyQ78. 2 / 2
| 2

reach
"Together, the data in XREF_FIG indicate that ataxin-3 functions through DnaJ-1 and stress inducible chaperones to reduce the toxicity of polyQ78 in Drosophila."

reach
"Together, the data in Fig. 5 indicate that ataxin-3 functions through DnaJ-1 and stress inducible chaperones to reduce the toxicity of polyQ78 in Drosophila."
ATXN3 affects poly-ubiquitin chains
| 2
ATXN3 binds poly-ubiquitin chains. 2 / 2
| 2

reach
"Ataxin-3 binds and cleaves poly-ubiquitin chains XREF_BIBR - XREF_BIBR."

reach
"The presence of two ubiquitin binding sites explains how ataxin-3 binds poly-ubiquitin chains and provides new insights into the molecular mechanism of ubiquitin recognition."
ATXN3 affects mutation
| 2
ATXN3 inhibits mutation. 2 / 2
| 2

sparser
"Here we identify an autosomal dominant inactivating mutation in the SLCO2A1 gene (p."

sparser
"Up to 30% of patients with hyperparathyroidism-jaw tumor syndrome, a rare form of multiple endocrine neoplasia resulting from autosomal dominant inactivating mutation of the Hrpt2 tumor suppressor gene, initially present with ossifying fibromas."
ATXN3 affects monoubiquitin
| 1 1
ATXN3 binds monoubiquitin. 2 / 2
| 1 1

sparser
"Atx3 can also bind monoubiquitin, and it does so through the multiple binding sites distributed along its length, albeit with low (20–400 μM) affinity."

reach
"Atx3 can also bind monoubiquitin, and it does so through the multiple binding sites distributed along its length, albeit with low (20-400 muM) affinity."
| 2
ATXN3 increases the amount of manganese atom. 2 / 2
| 2

reach
"ATXN3 interacts with and stabilizes the forkhead box O (FOXO) transcription factor FOXO4, and upon oxidative stress they both translocate to the nucleus and activate manganese superoxide dismutase (SOD2) transcription which in turn protects cells from oxidative damage."

reach
"Ataxin-3 has been shown to activate transcription factor forkhead box protein O4 (FOXO4) and induce the expression of manganese superoxide dismutase (SOD2), an antioxidant enzyme, under oxidative stress conditions; however, when ataxin-3 is expanded, this transcriptional activation effect is reduced and, in fact, SOD2 levels are decreased in SCA3 patients brain samples ."

sparser
"Pcsk9 mutations have been associated with autosomal dominant hypercholesterolemia, which is characterized by high LDL levels ( xref )."

sparser
"Population studies have shown that PCSK9 gain of function variants associate with high LDL-C levels and autosomal dominant hypercholesterolemia [ xref , xref ], whereas loss of function variants associate with low LDL-C levels [ xref , xref ]."
ATXN3 affects limB
| 2
| 2

sparser
"The 7q21.3-q22.1 region has been extensively studied because a locus determining an autosomal dominant form of a developmental limb abnormality, the split hand/split foot malformation ( SHFD1 ), was m[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Similarly, Adams-Oliver syndrome, a rare autosomal dominant disorder associated with terminal limb defects, may be caused by loss-of-function mutations in Notch1 and Dll4 as well as RBPJ ."
ATXN3 affects glucose
| 2
| 2

reach
"Crossing of the MCH and ataxin -3 mouse with the ob/ob mouse resulted in decreased body weight and significantly reduced blood glucose."

reach
"Decreased regional cerebral glucose metabolism was found in the cerebellum of all patients with SCA, the brainstem of SCA1, SCA2, SCA3, the thalamus and putamen of SCA3, and the parietal cortex of patients with SCA2."
ATXN3 affects endogenous DnaJ-1 protein
| 2
ATXN3 increases the amount of endogenous DnaJ-1 protein. 2 / 2
| 2

reach
"qRT-PCR results are mirrored by western blots from dissected fly heads that express polyQ78, where we observed that wild-type ataxin-3 leads to higher levels of endogenous DnaJ-1 protein."

reach
"qRT-PCR results are mirrored by western blots from dissected fly heads that express polyQ78, where we observed that wild-type ataxin-3 leads to higher levels of endogenous DnaJ-1 protein (XREF_FIG)."
ATXN3 affects endocrine
| 2
ATXN3 binds endocrine. 2 / 2
| 2

sparser
"Although non-endocrine tumors predominate in the DICER1 syndrome ( Figure 1 ), a multiple endocrine neoplasia phenotype is associated with this autosomal dominant familial cancer syndrome."

sparser
"Another autosomal dominant hamartomatous disorder associated with endocrine tumour development is Peutz-Jeghers syndrome (PJS)."
ATXN3 affects dynein
| 1
ATXN3 binds dynein. 1 / 2
| 1

reach
"In addition, ataxin-3 interacts with dynein and the cytosolic histone deacetylase 6 during transport of misfolded proteins in cultured cells, thus linking ataxin-3 to cytoskeletal transport processes."
ATXN3 affects csnB
| 2
| 2

sparser
"We report the results of molecular genetic analysis of an Irish family segregating an autosomal dominant form of CSNB in which a previously unreported threonine-to-isoleucine substitution at codon 94 in the rhodopsin gene was found to segregate with the disease."

sparser
"The clinical description, pedigree, dark adaptation and elctroretinogram (ERG) studies indicate that the patients have an autosomal dominant form (ad) of CSNB."
ATXN3 affects crystallin
| 2

sparser
"To date, many mutations in 12 human crystallin genes have been associated with inherited autosomal dominant (AD) and/or autosomal recessive (AR) cataract."

sparser
"Of these, mutations in 9 crystallin genes have been associated with autosomal dominant cataracts ( CRYAA , CRYAB , CRYBB1 , CRYBB2 , CRYBA1/A3 , CRYBA4 , CRYGC , CRYGD , and CRYGS ) but only 3 with autosomal recessive cataracts ( CRYAA , CRYBB1 , and CRYBB3 )."
ATXN3 affects calcium(2+)
| 2
| 2

reach
"Different ataxin-3 amyloid aggregates induce intracellular Ca (2+) deregulation by different mechanisms in cerebellar granule cells."

reach
"However, only the pre-fibrillar aggregates of expanded ATX3 (the only variant which forms bundles of mature fibrils) triggered a characteristic Ca (2+) response at a later stage that correlated with a larger hydrophobic exposure relative to the two other variants."
| 2

eidos
"ATXN3 depletion impairs autophagic degradation of long-lived proteins and increases the number of autophagosomes To explore whether human ATXN3 is involved in autophagy , we analyzed the autophagic flux directly by determining the fraction of long-lived intracellular proteins that were either degraded by basal or starvation-induced autophagy in control or ATXN3-depleted cells ."

eidos
"ATXN3 depletion increases the number of autophagosomes and impairs degradation of long-lived proteins ."
ATXN3 affects arginine
| 2
VCP binds ATXN3 and arginine. 2 / 2
| 2

reach
"VCP binds ataxin-3 at an arginine rich region that precedes its polyQ portion (XREF_FIG)."

reach
"VCP is bound directly by ataxin-3 through an arginine rich area preceding the polyQ repeat."
ATXN3 affects activity
| 2
ATXN3 inhibits activity. 2 / 2
| 2

sparser
"Atxn3 binds to p300 and inhibits its acetyltransferase activity (Li et al. xref )."

sparser
"Similarly, inhibition of proteasome activity by ataxin-3 with an expanded CAG tract has been demonstrated in C. elegans using an EGFP–degron reporter [ xref ]."
ATXN3 affects YUH1
| 2
| 2

sparser
"Although UCH is associated with MINDY and MJD based on the functional categories, the high number of catabolic functions makes it strictly different from the others."

sparser
"However, the papain fold is also seen in many non-DUB proteases and our HMM-to-HMM comparisons shown in xref and xref reveal significant sequence relationships only between some DUB and UbL-protease families such as ATX3-UCH or ZUFSP-UFSP-ATG4, leaving other families unconnected."
ATXN3 affects XPO1
| 1 1
| 1 1

reach
"In order to further exclude a possible direct interaction between CRM1 and ataxin-3, we took advantage of the observation that Ran in a complex with a transport receptor is resistant to the activity o[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In order to further exclude a possible direct interaction between CRM1 and ataxin-3, we took advantage of the observation that Ran in a complex with a transport receptor is resistant to the activity o[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
ATXN3 affects VCP, and arginine
| 2
VCP binds ATXN3 and arginine. 2 / 2
| 2

reach
"VCP binds ataxin-3 at an arginine rich region that precedes its polyQ portion (XREF_FIG)."

reach
"VCP is bound directly by ataxin-3 through an arginine rich area preceding the polyQ repeat."
ATXN3 affects USP13
1 |
1 |

No evidence text available
ATXN3 affects UBE2L3
1 | 1
1 | 1

No evidence text available

sparser
"Parkin, its cognate E2 UbcH7, and Ataxin-3 form a tight complex preventing the autoubiquitination of Parkin and the release of UbcH7 [ xref ]."
ATXN3 affects TWSG1
| 2
| 2

sparser
"Cowden syndrome (CS), an autosomal dominant disease associated with mutations in the PTEN tumor suppressor gene on chromosome 10q10.22-23, is associated with elevated risks of breast and thyroid cancer, as well as diverse benign neoplasms including gastrointestinal hamartomatous polyps, lipomas, giant fibroadenomas of the breast, hemangiomas, and multiple early-onset uterine leiomyomas [ xref ]."

sparser
"Germline truncating mutation of the tumor suppressor gene is associated with an autosomal dominant colon cancer predisposition syndrome known as familial adenomatous polyposis coli, or FAP."
ATXN3 affects TWNK
| 2
| 2

sparser
"The PEO1 ( C10ORF2 ) mutations are associated with autosomal dominant progressive external ophthalmoplegia (PEO) (PEOA3), parkinsonism, as well as infantileonset spinocerebellar ataxia. xref , xref , xref Biochemical analyses of purified mutant PEO1 proteins have been performed, but the results have been mixed. xref , xref , xref The functional defects of mutant Twinkle proteins have also been studied in the ortholog in Drosophila . xref Transgenic mice carrying a point mutation at amino acid residue 360 (A360T), or a short repeat of amino acids 353 to 365 reproduced histopathology and histochemical changes resulting from mitochondrial respiratory chain dysfunction."

sparser
"TWNK was also associated with autosomal dominant progressive external ophthalmoplegia (adPEO, OMIM #609286) which can result in ophthalmoparesis with exercise intolerance, ataxia, peripheral neuropathy and multiple mtDNA deletions and at least 40 causative mutations for adPEO have been identified up to now [ xref ]."
ATXN3 affects TTR
| 2
| 2

sparser
"More than 80 TTR mutations have been associated with autosomal dominant amyloidosis, usually presenting with peripheral and autonomic neuropathy and/or cardiomyopathy."

sparser
"Subsequent sequencing of the patient's TTR gene identified a pathogenic variant that is associated with autosomal dominant hereditary transthyretin-mediated amyloidosis."
ATXN3 affects TPM3
| 2
| 2

sparser
"Using this approach we found that certain rhodopsin point mutants in TM1 and TM5 that are associated with autosomal dominant retinitis pigmentosa, reconstitute into vesicles as monomers but retain wild-type (WT)-like scramblase activity xref ."

sparser
"Autosomal dominant nemaline myopathy associated with a missense mutation in the TPM3 gene has been identified in one large family."
ATXN3 affects TP53BP1
| 2
| 2

reach
"Consistent with defective DSB induced ubiquitylation, we found that depletion of ataxin-3 also significantly reduced both BRCA1 (Fig XREF_FIG D) and 53BP1 accumulation (Fig XREF_FIG E) to laser inflicted DNA damage, as well as to ionizing radiation induced foci (XREF_SUPPLEMENTARY and XREF_SUPPLEMENTARY)."

reach
"Indeed, we found that over-expression of ataxin-3 in control cells already significantly reduced accrual of 53BP1 (XREF_SUPPLEMENTARY)."
ATXN3 affects TOMM20L
2 |
2 |

No evidence text available

No evidence text available
ATXN3 affects TFAP2B
| 2
| 2

sparser
"Mutations in the gene encoding AP-2β are associated with Char syndrome, a human autosomal dominant disorder."

sparser
"Char syndrome is a rare autosomal dominant form of PDA with additional hand abnormalities and facial dysmorphisms caused by mutations in the TFAP2B gene ( xref , xref )."
ATXN3 affects TEX11
1 1 |
1 1 |

No evidence text available

No evidence text available
ATXN3 affects TCF4
| 1 1
| 1 1

reach
"Genes that were part of this signature on the brain side were the TCF4, ATN1, PPP2R2B, ATXN10 and ATXN3, which are associated with neurodegenerative and developmental disorders such as Pitt-Hopkins Syndrome [XREF_BIBR], Dentatorubral pallidoluysian atrophy [XREF_BIBR], SCA12 [XREF_BIBR], SCA10 [XREF_BIBR] and SCA3 [XREF_BIBR]."

sparser
"Heat-shock transcription factor 4 (HSF4) mutations are associated with autosomal dominant lamellar cataract and Marner cataract."
ATXN3 affects TARDBP
| 2
| 2

sparser
"Autosomal dominant mutations in TDP-43 are associated with sporadic and familial ALS xref , xref , and the redistribution of TDP-43 from the nucleus to cytoplasm has been recognized as a pathological hallmark for most forms of ALS and most frequent subtypes of FTD xref , xref ."

sparser
"VCP appears to interact with Ataxin-3 and TDP-43 neuronal inclusions [31,32] , interacts with and promotes clearance of folding-deficient V2 vasopressin receptor mutants that cause X-linked nephrogen[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
ATXN3 affects STB
| 2
| 2

reach
"STB/HAP1 can also interact with the causal agents of some other polyQ diseases, such as with Abelson helper integration site 1 in Joubert syndrome (Sheng et al., 2008), ataxin 3 in Machado-Joseph disease (Takeshita et al., 2011) and TATA binding protein in spinocerebellar ataxia type 17 (Prigge and Schmidt, 2007)."

reach
"The results clearly showed that HAP1 and STB interacts with the normal ataxin-3 through Josephin domain and polyglutamine expanded mutants derived from SCA3 as well."
ATXN3 affects SERPINH1
| 1 1
| 1 1

sparser
"Hence it seems unlikely that serpinH1 and ataxin-3 interaction plays an important functional role in SCA3, but may rather connect ataxin-3 localization to the ER."

reach
"Hence it seems unlikely that serpinH1 and ataxin-3 interaction plays an important functional role in SCA3, but may rather connect ataxin-3 localization to the ER."
ATXN3 affects S100A6
| 2
| 2

sparser
"There are several DNA tests currently available that are based on dominant or codominant mutations, including the RHO mutation for an autosomal dominant form of PRA in the English Mastiff offered by OptiGen () (Kijas et al. xref ), the mutation in HSF4 that is associated with hereditary cataract in the Australian Shepherd that is offered by the Animal Health Trust () (Mellersh et al. xref , xref ), and the polymorphisms associated with renal dysplasia in multiple breeds that is offered by DoGenes () (Whiteley et al. xref )."

sparser
"The only autosomal dominant form of PRA described so far has been reported in the Bull Mastiff and English Mastiff breeds."
ATXN3 affects RUNX1
| 2
| 2

sparser
"Deleterious mutations in RUNX1 (Runt-related transcription factor 1; MIM ID *151385) are associated with a rare Autosomal Dominant hereditary cancer syndrome (linked to 21q22.12) that is characterized by qualitative and quantitative platelet defects with a high-propensity for myelodysplastic syndrome (MDS), acute myeloid leukemia (AML), and lymphoid malignancies( xref )."

sparser
"In humans, heterozygous RUNX1 mutation is associated with an autosomal dominant disorder, the familial platelet disorder with predisposition to acute myeloid leukemia (FPD/AML) (Mendelian Inheritance in Man [MIM] 601399), characterized by impaired megakaryopoiesis, quantitative and qualitative defects in platelet function, and over 40% risk of development of myelodysplastic syndrome (MDS) or AML at a median age of 33 years [ xref – xref ]."
ATXN3 affects RRAGA
| 2
| 2

sparser
"Herein, we report that mutations in the RagA GTPase ( RRAGA ), a key regulator of the mechanistic rapamycin complex 1 (mTORC1), are associated with autosomal dominant cataracts."

sparser
"Our findings thus indicate that the RRAGA mutations are associated with autosomal dominant cataracts through disruption of the mTORC1 pathway, a substantial modulator of ageing and age-related human diseases [ xref ]."
ATXN3 affects RAD23A, and RAD23B
| 2
| 2

sparser
"It has been reported that ATX3 interacts with HHR23A and HHR23B, homolog of DNA repair protein RAD23 ( xref ), and that ATX3 can be recruited to the DNA damage sites induced by laser microirradiation ( xref )."

sparser
"Initial studies found that ataxin-3 interacts with two proteins, HHR23A and HHR23B, that are both homologs of the DNA repair protein Rad23 [ xref ]."
| 1 1

eidos
"ATXN3 promotes prostate cancer progression by stabilizing YAP ."

reach
"In addition, ATXN3 depletion significantly decreased PC cell proliferation, invasion and stem-like properties."
ATXN3 affects PSEN1
| 2
| 2

sparser
"AD is sporadic (sAD) in the great majority of cases, but 1–5% of Alzheimer patients suffer from the autosomal dominant form of the disease (ADAD), which is caused by mutations in the presenilin 1 ( PSEN1 ), presenilin 2 ( PSEN2 ), or amyloid precursor protein ( APP ) genes xref ."

sparser
"Seizures and myoclonus is common feature of autosomal dominant EOAD, which was associated with PSEN1 mutation [ xref , xref ]."
ATXN3 affects PROM1
| 2
| 2

sparser
" PROM1 (OMIM 612657) codes for a transmembrane glycoprotein (Prominin-1) that localizes to the base of the rod and cone outer segments and is involved in disc assembly and maintenance of outer segment structure. xref PROM1 is associated with both autosomal dominant (AD) xref , xref and AR xref retinal dystrophies, with the AD forms tending to be later onset and milder. xref PROM1 is responsible for between 1.0% and 9.5% of AR CRD (arCRD) cases. xref , xref , xref "

sparser
"Missense mutations in the PROM1 gene have mostly been associated with autosomal dominant forms of Stargardt-like macular dystrophy, bull’s eye macular dystrophy, and cone-rod dystrophy."
| PMC
ATXN3 affects POLR2A
| 2
HTT binds ATXN3 and POLR2A. 2 / 2
| 2

sparser
"PNKP has been shown to promote transcription-coupled double-strand break repair ( xref ) and often performs transcription-coupled repair when in complex with huntingtin protein, RNA polymerase II subunit A, ataxin-3, and cyclic AMP-response element binding protein (CBP) ( xref )."

sparser
"The mechanisms for this were recently suggested to be through direct interactions between HTT and RNA polymerase II subunit A (POLR2A), ataxin-3, the DNA repair enzyme polynucleotide-kinase-3'-phosphatase (PNKP), and cyclic AMP-response element-binding (CREB) protein (CBP) [ xref ], as well as complexes with the DNA double-strand break repair complex Ku70/Ku80 [ xref ]."
ATXN3 affects PML
2 |
2 |

No evidence text available

No evidence text available
ATXN3 affects PLD
| 2
ATXN3 binds PLD. 2 / 2
| 2

sparser
"The most common form of PLD is associated to Autosomal Dominant Polycystic Kidney Disease (ADPKD), a genetic disease affecting more than six million people worldwide xref , xref ."

sparser
"Interestingly, PLD is usually associated with a renal anomaly called autosomal dominant polycystic kidney disease (ADPKD), suggesting a certain universal mechanism underlying tubular formation and/or function among different organs."
ATXN3 affects PKD1
| 2
| 2

sparser
"Hypertension and aneurysm are frequently associated with autosomal dominant polycystic kidney disease (ADPKD) caused by polycystin-1 (PC1) mutations, which is closely related to endothelial dysfunction."

sparser
"Mutation of a ciliary protein, polycystin-1 (PC1) is associated with autosomal dominant polycystic kidney disease."
ATXN3 affects PITX2
| 2
| 2

sparser
"Furthermore, the altered pitx2 expression pattern, together with the described morphological features of the lens-ablated fish suggests that Astyanax mexicanus could be considered as an alternative teleost model organism in which to study Axenfeld-Rieger syndrome (ARS), a rare autosomal dominant developmental disorder that is associated with PITX2 and which has both ocular and non-ocular abnormalities."

sparser
"Several studies have reported that mutation of PITX2 is associated with the pathogenesis of Axenfeld-Rieger syndrome (ARS) which is an autosomal dominant human disease characterized by developmental defects of eye, teeth and heart xref ."
ATXN3 affects PICK1
1 1 |
1 1 |

No evidence text available

No evidence text available
ATXN3 affects PHEX
| 2
| 2

sparser
"The list of the diseases associated with OPLL includes hypophosphatemic rickets/osteomalacia, including an autosomal dominant form (MIM 193100) caused by FGF23 mutations, an X-linked dominant form (MIM 307800) caused by PHEX mutations, an X-linked recessive form (MIM 300554) caused by CLCN5 mutations, and autosomal recessive forms caused by DMP1 (MIM 600980) and ENPP1 (MIM 173335) mutations."

sparser
"Several forms of hypophosphatemic rickets are known, including an X-linked form (MIM 307800) caused by phosphate regulating endopeptidase homolog, X-linked ( PHEX ) mutations (MIM 300550), an autosomal dominant form (MIM 193100) caused by fibroblast growth factor 23 ( FGF23 ) mutations (MIM 605380), an X-linked recessive form (MIM 300554) caused by chloride channel, voltage-sensitive 5 ( CLCN5 ) mutations (MIM 300008), and autosomal recessive forms caused by dentin matrix acidic phosphoprotein 1 ( DMP1 ) (MIM 600980), hypophosphatemic rickets, autosomal recessive 2 ( ARHR2 ) (MIM 613312) or ectonucleotide pyrophosphatase/phosphodiesterase 1 ( ENPP1 ) (MIM 173335) mutations. 'tiptoe walking' ( TTW ) mouse, which has a spontaneous nonsense mutation in ENPP1 is a good model for OPLL.[ xref ] Also, OPPL is a frequent complication in patients with endocrine disorders including hypoparathyroidism[ xref ] and acromegaly/gigantism.[ xref ] However, most cases of OPLL are idiopathic."
ATXN3 affects PHA
| 2
| 2

sparser
"TM The autosomal dominant form of PHA presents with an asymptomatic to moderate clinical picture and mineralocorticoid resistance restricted only to the kid- ney."

sparser
"This is an autosomal dominant form of PHA1."

sparser
"Pcsk9 mutations have been associated with autosomal dominant hypercholesterolemia, which is characterized by high LDL levels ( xref )."

sparser
"Population studies have shown that PCSK9 gain of function variants associate with high LDL-C levels and autosomal dominant hypercholesterolemia [ xref , xref ], whereas loss of function variants associate with low LDL-C levels [ xref , xref ]."

reach
"In addition, ATXN3 depletion significantly decreased PC cell proliferation, invasion and stem-like properties."

eidos
"In addition , ATXN3 depletion significantly decreased PC cell proliferation , invasion and stem-like properties ."
ATXN3 affects NOS2
| 2
| 2

reach
"We thus sought to determine whether ataxin-3 interacted with the CHIP substrate GFP INOS."

reach
"In lysates from transfected cells, immunoprecipitation of ataxin-3 confirmed that ataxin-3 interacts with GFP INOS in cells (XREF_FIG)."
ATXN3 affects MUC1
| 2
| 2

sparser
"Certain hereditary kidney diseases cannot be diagnosed by using exome sequencing (eg, MUC1-autosomal dominant tubulointerstitial kidney disease)."

sparser
"For example, causal variants in the MUC1 gene, which is associated with autosomal dominant tubulointerstitial kidney disease (ADTKD- MUC1 ; OMIM 174000) and contains a highly repetitive, GC-rich sequence, were missed by NGS-based regional capture, WES, and WGS and were identified only by long-range PCR and molecular cloning xref ."
ATXN3 affects MGRN1
2 |
2 |

No evidence text available

No evidence text available
ATXN3 affects MED12
| 2
| 2

sparser
"In two large Italian families with autosomal dominant OPA1 mutations, adCPEO was associated with parkinsonism in several patients, whereas symptomatic optic atrophy was absent in most of the affected individuals (Carelli et al xref )."

sparser
"For example in the case of OPA1, the autosomal dominant mutations are associated with reduced OPA1 expression (Bonifert et al , xref ), while for the recessive form almost absent protein expression was found (Spiegel et al , xref )."
ATXN3 affects MAPT
| 2
| 2

sparser
"Initial translational efforts might, therefore, be more successful in disorders with more homogeneous underlying pathology, such as the primary tauopathies, progressive supranuclear palsy (PSP) and autosomal dominant frontotemporal lobar degeneration (FTLD) associated with MAPT mutations, in all of which tau is the major abnormal protein."

sparser
"The potential role of tau in various frontotemporal dementias has been further strengthened with recent reports of several autosomal dominant hereditary forms of frontotemporal dementia (sometimes ass[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
ATXN3 affects LMNA
| 2
| 2

sparser
"Asp300Gly) in the LMNA protein has been associated with an autosomal dominant late-onset cardiocutaneous progeria [ xref ]."

sparser
"FPLD (MIM 151660) is a rare autosomal dominant form of insulin resistance caused by mutant LMNA, which encodes nuclear lamin A/C, a structural component of the nuclear envelope.[ xref ] FPLD is characterized by loss of fat affecting the limbs and trunk, accumulation of fat in the neck and face, and predisposition to insulin resistance, leading to complications such as glucose intolerance, dyslipidemia, high blood pressure, liver steatosis, and increased risk for coronary heart disease."
ATXN3 affects LIPC
| 2
| 2

sparser
"In autosomal dominant forms of syndromic HL which tend to have a variable expression, a key feature for the diagnosis may be found in a relative rather than in the proband."

sparser
"DFNA5 was originally identified as a gene responsible for an autosomal dominant form of HL in a Dutch family (Van Laer et al., xref )."
ATXN3 affects LC3C
| 2
ATXN3 binds LC3C. 2 / 2
| 2

reach
"Moreover, Ataxin-3 interacts with the autophagosomal membrane proteins LC3C and GABARAP and transiently localized to early autophagosomes."

reach
"The Machado-Joseph disease deubiquitylase ataxin-3 interacts with LC3C and GABARAP and promotes autophagy."
ATXN3 affects LC3-II
| 2
ATXN3 increases the amount of LC3-II. 2 / 2
| 2

reach
"Moreover, ataxin-3 knockdown in HeLa cells lowered LC3-II levels and increased the levels of the autophagy substrate, p62 (XREF_FIG)."

reach
"Ataxin-3 knockdown in primary neurons (XREF_FIG) lowered LC3-II levels in the presence of bafilomycin A1 (BafA1)."
ATXN3 affects KIT
| 2
| 2

sparser
"In humans, c-kit mutation is associated with an autosomal dominant disorder of melanocyte development, piebaldism, which is characterized by leukoderma and poliosis."

sparser
"However, germline autosomal dominant mutations of KIT have been associated with familial multiple GISTs in paediatric patients [ xref ]."
ATXN3 affects JAG1
| 2
| 2

sparser
"Mutations in the human homologue of Jag1 cause Alagille syndrome (AGS), an autosomal dominant disorder associated with liver, heart, eye and skeletal abnormalities, accompanied by a characteristic facies."

sparser
"Also, various mutations of the Notch ligand JAGGED1 have been associated with the autosomal dominant Alagille syndrome in which misshaped vertebrae (butterfly vertebrae) are observed (AGS [MIM 118450 ]) ( xref ; xref )."
ATXN3 affects Integrins
| 1 1
| 1 1

sparser
"Ataxin-3 interacts with α5 integrin subunit and represses its degradation."

reach
"Because ATXN3 interacts with and stabilizes alpha5 integrin in a DUB activity dependent manner, it most likely regulates the degradation of this protein through its role in ubiquitin dependent proteostasis."
ATXN3 affects IMMT
| 1 1
| 1 1

sparser
"In myeloid cells ataxin-3 associates with the mitochondrial cristae protein MIC60, and is required for oxidative phosphorylation."

reach
"In myeloid cells ataxin-3 associates with the mitochondrial cristae protein MIC60, and is required for oxidative phosphorylation."
ATXN3 affects IGHD
| 2
| 2

sparser
"These data indicate that the R183H mutant GH, although causing an autosomal dominant form of IGHD has an identical effect on GHR/GHBP transcription as its wild-type, the 22-kDa GH."

sparser
"In total, we identified pathogenic GH1 defects in 9 of 38 patients with IGHD (23%), eight with the autosomal recessive and one with the autosomal dominant inheritance form of IGHD."
ATXN3 affects IFN-I
| 2
ATXN3 activates IFN-I. 2 / 2
| 2

reach
"We clarify that ATXN3 does not promote IFN-I production, but enhances the IFN-I-mediated signaling pathway."

reach
"These findings reveal a novel biological function of ATXN3 and an important antiviral mechanism by which the deubiquitinase ATXN3 positively regulates IFN-I antiviral response, and they may provide a novel strategy for enhancing IFN based antiviral therapy."
ATXN3 affects HTT, and POLR2A
| 2
HTT binds ATXN3 and POLR2A. 2 / 2
| 2

sparser
"PNKP has been shown to promote transcription-coupled double-strand break repair ( xref ) and often performs transcription-coupled repair when in complex with huntingtin protein, RNA polymerase II subunit A, ataxin-3, and cyclic AMP-response element binding protein (CBP) ( xref )."

sparser
"The mechanisms for this were recently suggested to be through direct interactions between HTT and RNA polymerase II subunit A (POLR2A), ataxin-3, the DNA repair enzyme polynucleotide-kinase-3'-phosphatase (PNKP), and cyclic AMP-response element-binding (CREB) protein (CBP) [ xref ], as well as complexes with the DNA double-strand break repair complex Ku70/Ku80 [ xref ]."
ATXN3 affects HSF4
| 2
| 2

sparser
"Hsf4 mutations are associated with autosomal dominant lamellar and Marner cataract [ xref ]."

sparser
"In humans, mutations in HSF4 have been associated with both autosomal dominant and recessive cataracts."
ATXN3 affects HNF1A
| 2
| 2

sparser
"Heterozygous germline mutations in HNF1A are responsible for an autosomal dominant form of diabetes MODY3 (maturity onset diabetes of the young type 3)."

sparser
"Mutations in the HNF1alpha gene are associated with an autosomal dominant form of non-insulin-dependent diabetes mellitus called maturity-onset diabetes of the young (MODY3)."
ATXN3 affects HHR23
| 2
ATXN3 binds HHR23. 2 / 2
| 2

reach
"Ataxin-3 also associates with HHR23, the UPP-escort protein VCP and p97, UBXN-5 protein and with the proteasome, suggesting a role in the modulation of protein degradation XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR."

reach
"In addition to the 26S proteasome and ubiquitylated substrate, HHR23 also interacts with ataxin-3 in vitro, a protein involved in the development of the neurodegenerative Machado-Joseph disease [XREF_BIBR]."
ATXN3 affects HGF
| 2
| 2

sparser
"Linkage analysis of HGF patients revealed several chromosomal regions that may contain mutations responsible for isolated, non-syndromic autosomal dominant forms of HGF."

sparser
"Loci for isolated, non-syndromic autosomal dominant forms of HGF have been localized to chromosome 2p21-p22 in a Brazilian family [ xref ], and to chromosome 5q13-q22 [ xref ] and 11p15 in a number of Chinese families [ xref ]."
ATXN3 affects Gln-Gln
| 2
| 2

reach
"We noticed that more atx3 was bound to the p97 QQ than to wt p97 (XREF_FIG, lane 4 vs. 2; XREF_FIG)."

reach
"The expression of atx3 reduced the amount of ubiquitinated proteins associated with wt p97 (XREF_FIG, lane 2 vs. lane 1), but not of those bound by p97 QQ (lane 4 vs. lane 3), although more atx3 was bound by p97 QQ (lane 4 vs. lane 2)."
ATXN3 affects GST
| 2
| 2

sparser
"In contrast, a p97 mutant lacking its N-terminal domain (N-domain) failed to bind GST-atx3 ( xref )."

sparser
"Intriguingly, the results of a GST pulldown assay with purified GST-ATXN3 revealed that ATXN3 interacted with both His-TSPAN8 and His-PTCH1 (Supplementary Fig.  xref )."
ATXN3 affects GJB3
| 2
| 2

sparser
"In addition, heterozygous mutations in the GJB3 and KCNQ have been associated with autosomal dominant hearing loss [ xref , xref ]."

sparser
"Mutations in (OMIM: ) were originally shown to underlie an autosomal dominant form of non-syndromic deafness DFNA2 (OMIM:) in Chinese patients and the c."
ATXN3 affects GFI1B
| 2
| 2

sparser
"We now demonstrate that both autosomal dominant and autosomal recessive αδ-SPD are associated with mutations in GFI1B ."

sparser
"We now demonstrate that both autosomal dominant and autosomal recessive αδ-SPD are associated with mutations in GFI1B . Patients were enrolled in clinical protocol 76-HG-0238, “Diagnosis and treatmen[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
ATXN3 affects GCH1
| 2
| 2

sparser
"Mutations in the GCH1 gene are associated with levodopa responsive dystonia (DRD, also known as DYT5 dystonia), an autosomal dominant neurological movement disorder ( xref ; xref ; xref ; xref )."

sparser
"Finally, mutations causing the autosomal dominant form of dopa-responsive dystonia have been identified in the gene coding for GTP cyclohydrolase I. Mutations in tyrosine hydroxylase have been identified in two brothers and put forward as evidence of an autosomal recessive form of the disease."
ATXN3 affects FOXO
| 1 1
| 1 1

sparser
"Moreover, interactions of SCA3 with Foxo mediated by coiled-coil domains of these two proteins resulted in functional impairment of this transcription factor, whereas its overexpression significantly rescued the SCA3-induced defects."

reach
"ATXN3 interacts with and stabilizes the FOXO transcription factor FOXO4, and upon oxidative stress, they both translocate to the nucleus and activate manganese superoxide dismutase (SOD2) transcription, which in turn protects cells from oxidative damage."
ATXN3 affects FH
| 2
| 2

sparser
"Autosomal dominant mutations in FH are associated with hereditary leiomyomatosis and renal cell cancer, indicating that FH functions as a tumor suppressor ( xref ; xref )."

sparser
"HLRCC-associated RCC occurs in patients with HLRCC syndrome, which is an autosomal dominant hereditary disease that is associated with germline mutations in FH at chromosome 1q42.3-q43."
ATXN3 affects FGFR1
| 2
| 2

sparser
"Recently, loss-of-function mutations of fibroblast growth factor receptor-1 (FGFR1) were associated with an autosomal dominant form of Kallmann syndrome."

sparser
"Other different modes of KS transmission (autosomal dominant and recessive) have since been described [ xref ], among them the autosomal dominant form caused by mutation of the gene FGFR1 , initially defined as KAL2 gene [ xref ]."
ATXN3 affects EWSR1
1 1 |
1 1 |

No evidence text available

No evidence text available
ATXN3 affects Dnmt1, PRKN, and UBL
| 2
ATXN3 binds PRKN, Dnmt1, and UBL. 2 / 2
| 2

sparser
"For example, the interaction of the parkin Ubl domain with the UIM regions of the deubiquitinating protein ataxin-3 is required for the unique ubiquitin-editing role of ataxin-3 ( xref , xref )."

sparser
"The observation that the three UIM sites in ataxin-3 bind equally to a single site of the parkin Ubl domain suggests a multi- or polyvalent ligand binding mode."
ATXN3 affects Disease
| 2
ATXN3 activates Disease. 2 / 2
| 2

reach
"These findings suggest that anti-NP response at an early stage effectively controls infection and contributes to cross-protective immunity.A single immunization with ML29 fully protected guinea pigs and marmosets against fatal disease caused by LASV/JOS (lineage IV) and Nigerian strain 803213 (lineage II)46,88."

reach
"Expansion of polyQ domains in huntingtin and the deubiquitinase ataxin-3 causes Huntington’s disease characterized by loss of striatal neurons and hence changes in mood and personality, defective motor coordination, and involuntary movements and type-3 spinocerebellar ataxia (SCA3), a form of neurodegeneration in the striatum and cerebellum, respectively [104]."
| PMC
ATXN3 affects DSBs
| 2
ATXN3 binds DSBs. 2 / 2
| 2

reach
"An alternative explanation for the recruitment of ataxin-3 to DSBs is the presence of ubiquitylated chromatin, which could be recognized by the three UIMs present in ataxin-3."

reach
"The recruitment of ataxin-3 to DSBs is consistent with a recently reported systematic characterization of DUBs, which showed that ataxin-3 accumulates at laser inflicted DNA damage (Nishi etal, 2014)."
ATXN3 affects DND1
2 |
2 |

No evidence text available

No evidence text available
ATXN3 affects DICER1
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sparser
"Germline mutations in DICER1 are associated with a distinct autosomal dominant tumor predisposition syndrome (OMIM #601200), whereby patients have an increased risk of developing a variety of extremely rare tumors, such as pleuropulmonary blastoma and PBs."

sparser
"Germline mutations in DICER1 are associated with an autosomal dominant hereditary cancer predisposition syndrome that confers an increased risk for the development of several rare childhood and adult-onset tumors, the most frequent of which include pleuropulmonary blastoma, ovarian sex cord-stromal tumors, cystic nephroma, and thyroid gland neoplasia."
ATXN3 affects DHX40
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ATXN3 activates DHX40. 2 / 2
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"Notably, a smaller amount of ubiquitinated proteins was coprecipitated with p97 from cells expressing wt atx3 (XREF_FIG, lane 5 vs. lane 1; XREF_FIG, lane 3 vs. lane 1), suggesting that atx3 mediated PAD may also occur in intact cells."

reach
"Together, these data suggest that atx3 can promote PAD both in intact cells and in vitro."
ATXN3 affects CYP74A
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sparser
"Recently, mutations in ARHGAP31 and RBPJ have been found causing autosomal dominant forms of AOS."

sparser
"Autosomal dominant forms of AOS are linked to mutations in ARHGAP31, DLL4, NOTCH1 or RBPJ, while DOCK6 and EOGT underlie autosomal recessive inheritance."
ATXN3 affects CRX
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sparser
"This large deletion of the CRX gene is associated with a severe form of autosomal dominant retinal degeneration."

sparser
"Mutations in human CRX (NCBI Reference Sequence: NG_008605.1) have been associated with autosomal dominant forms of the retinal degenerative diseases Retinitis Pigmentosa (adRP), Cone-Rod Dystrophy (adCoRD) and Leber Congenital Amaurosis (adLCA), with different ages of onset and severity xref xref – xref ."
ATXN3 affects CREB1
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No evidence text available

No evidence text available
ATXN3 affects CRC
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sparser
"Two major autosomal dominant forms of heritable CRC are familial adenomatous polyposis (FAP) and Lynch syndrome (also known as hereditary nonpolyposis colorectal cancer)."

sparser
"Lynch syndrome (LS) or hereditary non-polyposis colorectal cancer, is an autosomal dominant form of CRC that may account for up to 5% of all CRC cases. xref The penetrance for development of malignancy is up to 68.7% in males and 52% in females. xref Founder effects for LS have been discovered in several populations, including Newfoundland, Canada. xref It is believed that the increased rates of CRC in this province, which are among the highest in the world, may be attributable to a high rate of familial cancer in the population and possibly to the presence of novel susceptibility genes. xref , xref Familial adenomatous polyposis is another autosomal dominant inherited syndrome that greatly increases the risk of CRC and founder effects have been established. xref , xref , xref "
ATXN3 affects CK2beta
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ATXN3 binds CK2beta. 2 / 2
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"(2) In 293 cells, both wild type and expanded ataxin-3 interacted with CK2beta, but not CK2alpha."

reach
"Results (1) Both wild type and expanded ataxin-3 interacted with CK2alpha and CK2beta in vitro."
ATXN3 affects CK2alpha
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ATXN3 binds CK2alpha. 2 / 2
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sparser
"Results (1) Both wild type and expanded ataxin-3 interacted with CK2alpha and CK2beta in vitro. (2) In 293 cells, both wild type and expanded ataxin-3 interacted with CK2beta, but not CK2alpha. (3) CK2 phosphorylated wild type and expanded ataxin-3."

sparser
"ATXN3 associated with CK2alpha and pharmacological inhibition of CK2 decreased nuclear ATXN3 levels and the formation of nuclear inclusions."
ATXN3 affects CK2
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| 1 1

sparser
"The interaction between ataxin-3 and CK2 was identified by glutathione S-transferase (GST) pull-down assay and co-immunoprecipition assay."

reach
"The interaction between ataxin-3 and CK2 was identified by glutathione S-transferase (GST) pull-down assay and co-immunoprecipition assay."
ATXN3 affects CHMP4B
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sparser
"Interestingly, a CHMP4B mutation associated with autosomal dominant posterior polar cataract abolishes the ability of CHMP4B to localize to micronuclei."

sparser
"As a CHMP4B mutation associated with an autosomal dominant form of cataract abolishes the ability of CHMP4B to localize to micronuclei, the autophagic degradation of DNA is implicated in the protection of lens cells from cataract development."
ATXN3 affects CHI3L1
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sparser
"Using EM and AFM, we show that at 37°C ataxin-3 forms oligomers with diameters of ∼7–11 nm, which seem to further assemble into filaments."

sparser
"55 Non-pathological recombinant ataxin-3 forms oligomers in vitro , displaying a pathogenic conformation, under physiological conditions."
ATXN3 affects CDK5RAP2
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sparser
"Three of the 12 were compatible with the autosomal dominant form of microcephaly with chorioretinopathy (MIM 156590), possibly as a new mutation."

sparser
"This appears to be a first report of a autosomal dominant form of microcephaly associated with mild to moderate mental retardation in contrast to absent or mild mental retardation described in earlier reports."
ATXN3 affects CASR
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sparser
"CaSR abnormalities are associated with three hypocalcemic disorders, which are autosomal dominant hypocalcemia (ADH), Bartter syndrome type V and autoimmune hypoparathyroidism ()."

sparser
"However, it may be that gain-of-function CaSR mutations, which cause autosomal dominant hypocalcemia (ADH) ( xref ), are associated with abnormalities of glucose homeostasis and not FHH-associated loss-of-function CaSR mutations."
ATXN3 affects CAPN5
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sparser
"CAPN5 has been associated with autosomal dominant neovascular inflammatory vitreoretinopathy (ADNIV) [ xref – xref ], obesity [ xref ], Huntington’s disease [ xref , xref ], and polycystic ovary syndrome [ xref ]."

sparser
"Mutations in CAPN5 are associated with the devastating retinal degenerative disease autosomal dominant neovascular inflammatory vitreoretinopathy (ADNIV). xref xref – xref ADNIV is a hereditary autoimmune disease of the eye that is characterized by abnormal retinal pigmentation, retinal neovascularization, photoreceptor degeneration, vitreous hemorrhage, intraocular fibrosis, and tractional retinal detachment."
ATXN3 affects C9orf72
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| 1 1

reach
"Further studies, particularly on the interaction between C9orf72 and ataxin-3, are needed to verify our results."

sparser
"Further studies, particularly on the interaction between C9orf72 and ataxin-3, are needed to verify our results."
ATXN3 affects BSCL2
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sparser
"ERPO may represent an ER quality control pathway for multiple ER substrates, since aggregation-prone mutant forms of seipin that are associated with an autosomal dominant motor neuron disease, accumulate in the same compartment [ xref ]."

sparser
"Mutant VAPB did not codistribute with mutant forms of seipin that are associated with an autosomal dominant motor neuron disease, and accumulate in a protective ER derived compartment termed ERPO (ER protective organelle) in neurons."
ATXN3 affects BEST1
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sparser
"Best vitelliform macular dystrophy (BVMD) is autosomal dominant juvenile onset maculopathy, which is associated with a mutation in human BEST1 gene.[ xref ] hBEST1 gene encodes bestrophin-1 (Best1) protein, which is expressed basolaterally in retinal pigment epithelium (RPE) [ xref ] and in astrocytes.[ xref ] BVMD involves several stages and leads to loss of central vision."

sparser
"BEST1 is associated with autosomal dominant vitreoretinochoroidopathy, vitelliform macular dystrophy, and autosomal-recessive bestrophinopathy xref ."
| PMC
ATXN3 affects BCL2
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ATXN3 decreases the amount of BCL2. 2 / 2
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reach
"Moreover, polyQ expanded ataxin-3 was found to decrease mRNA and protein levels of the prosurvival Bcl-2 by affecting Bcl-2 mRNA stability [XREF_BIBR, XREF_BIBR]."

reach
"Full-length expanded ataxin-3 enhances mitochondrial mediated cell death and decreases Bcl-2 expression in human neuroblastoma cells."
ATXN3 affects BAG3
1 | 1
1 | 1

No evidence text available

sparser
"Bag3 mutations in humans are associated with autosomal dominant forms of myofibrillar myopathy and dilated cardiomyopathy (Selcen et al., xref ; Norton et al., xref )."
ATXN3 affects Ataxia
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ATXN3 activates Ataxia. 2 / 2
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"Transcriptional dysregulation has been described in spinocerebellar ataxia type 3/Machado-Joseph disease (SCA3/MJD), an autosomal dominant ataxia caused by a polyglutamine expansion in the ataxin-3 protein."

reach
"Mutation of USP14 in mice or ataxin-3 in humans causes ataxia (Crimmins et al., 2006; Duenas et al., 2006) , whereas the S18Y allele of human UCH-L1 confers protection against sporadic Parkinson's disease."
ATXN3 affects ATXN1
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reach
"Interestingly, interaction of Atx1 and Atx3 with other proteins bearing polyQ revealed that the activity of Atx2 (SCA2) is critical for SCA3 and SCA1 pathogenesis [XREF_BIBR, XREF_BIBR]."

reach
"We also analyzed the possibility of genetic interactions between ATXN1 and ATXN2, ATXN2 and ATXN3, and ATXN2 and CACNA1A."
ATXN3 affects ARHGAP19
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1 1 |

No evidence text available

No evidence text available
ATXN3 affects APEX1
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reach
"The results clearly showed that HAP1 and STB interacts with the normal ataxin-3 through Josephin domain and polyglutamine expanded mutants derived from SCA3 as well."

reach
"STB/HAP1 can also interact with the causal agents of some other polyQ diseases, such as with Abelson helper integration site 1 in Joubert syndrome (Sheng et al., 2008), ataxin 3 in Machado-Joseph disease (Takeshita et al., 2011) and TATA binding protein in spinocerebellar ataxia type 17 (Prigge and Schmidt, 2007)."
ATXN3 affects AEBP2
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No evidence text available

No evidence text available
ATXN3 affects ACOD1
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sparser
"A mutation in the human myocyte enhancer factor 2A ( MEF2A ) gene, which is a member of the myocyte enhancer family of transcription factors, has previously been detected in an autosomal dominant form of CAD ( xref )."

sparser
"Recently, a missense mutation in the low density lipoprotein receptor related protein 6 (LRP6) gene, encoding low density lipoprotein receptor related protein 6, has been implicated in an autosomal dominant form of early-onset CAD."
ATXN2 affects CSA
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sparser
"In this study we show that MID1 can also bind to the CAG repeat region of ATXN2, ATXN3 , and ATXN7 in vitro , suggesting that binding of MID1 to CAG repeats is not dependent on the flanking regions."

sparser
"We show that ATXN2, ATXN3 , and ATXN7 bind to MID1 in a CAG repeat length-dependent manner."
ARHGAP19 affects ATXN3
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1 1 |

No evidence text available

No evidence text available
| 2

sparser
"Familial early-onset AD (FAD) is associated with autosomal dominant mutations in the amyloid precursor protein (APP) and in the catalytic subunits (presenilin 1 and presenilin 2) of the intramembrane protease that processes it, γ-secretase ( xref )."

sparser
"Early-onset autosomal dominant AD genes are associated with excessive accumulation, however cognitive impairment best correlates with NFTs and disrupted microtubules."
APP affects AD
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APP binds ATXN3 and AD. 2 / 2
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sparser
"There are only two dozen families carrying autosomal dominant APP mutations associated with early-onset AD."

sparser
"Among the early-onset AD cases, few carry a rare familial form of AD with an inheritable autosomal dominant mutation in either amyloid precursor protein (APP), presenilin-1 or presenilin-2 genes."
APEX1 affects ATXN3
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| 2

reach
"The results clearly showed that HAP1 and STB interacts with the normal ataxin-3 through Josephin domain and polyglutamine expanded mutants derived from SCA3 as well."

reach
"STB/HAP1 can also interact with the causal agents of some other polyQ diseases, such as with Abelson helper integration site 1 in Joubert syndrome (Sheng et al., 2008), ataxin 3 in Machado-Joseph disease (Takeshita et al., 2011) and TATA binding protein in spinocerebellar ataxia type 17 (Prigge and Schmidt, 2007)."
AP1S2 affects PHEX
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sparser
"The list of the diseases associated with OPLL includes hypophosphatemic rickets/osteomalacia, including an autosomal dominant form (MIM 193100) caused by FGF23 mutations, an X-linked dominant form (MIM 307800) caused by PHEX mutations, an X-linked recessive form (MIM 300554) caused by CLCN5 mutations, and autosomal recessive forms caused by DMP1 (MIM 600980) and ENPP1 (MIM 173335) mutations."

sparser
"Several forms of hypophosphatemic rickets are known, including an X-linked form (MIM 307800) caused by phosphate regulating endopeptidase homolog, X-linked ( PHEX ) mutations (MIM 300550), an autosomal dominant form (MIM 193100) caused by fibroblast growth factor 23 ( FGF23 ) mutations (MIM 605380), an X-linked recessive form (MIM 300554) caused by chloride channel, voltage-sensitive 5 ( CLCN5 ) mutations (MIM 300008), and autosomal recessive forms caused by dentin matrix acidic phosphoprotein 1 ( DMP1 ) (MIM 600980), hypophosphatemic rickets, autosomal recessive 2 ( ARHR2 ) (MIM 613312) or ectonucleotide pyrophosphatase/phosphodiesterase 1 ( ENPP1 ) (MIM 173335) mutations. 'tiptoe walking' ( TTW ) mouse, which has a spontaneous nonsense mutation in ENPP1 is a good model for OPLL.[ xref ] Also, OPPL is a frequent complication in patients with endocrine disorders including hypoparathyroidism[ xref ] and acromegaly/gigantism.[ xref ] However, most cases of OPLL are idiopathic."
AM146 affects ATXN3
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AM146 inhibits ATXN3. 2 / 2
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"UCHL1, UCHL3, USP2 and USP8 were found to be inhibited by AM146, RA-9, and RA-14, which did not inhibit Ataxin-3, A20, BAP1, Otubain 1 or USP7."

reach
"UCHL1, UCHL3, USP2 and USP8 were found to be inhibited by AM146, RA-9, and RA-14 which did not inhibit Ataxin-3, A20, BAP1, Otubain 1 or USP7 [XREF_BIBR]."
AEBP2 affects ATXN3
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No evidence text available

No evidence text available
AD affects APP, and ATXN3
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APP binds ATXN3 and AD. 2 / 2
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sparser
"There are only two dozen families carrying autosomal dominant APP mutations associated with early-onset AD."

sparser
"Among the early-onset AD cases, few carry a rare familial form of AD with an inheritable autosomal dominant mutation in either amyloid precursor protein (APP), presenilin-1 or presenilin-2 genes."
ACOD1 affects ATXN3
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| 2

sparser
"A mutation in the human myocyte enhancer factor 2A ( MEF2A ) gene, which is a member of the myocyte enhancer family of transcription factors, has previously been detected in an autosomal dominant form of CAD ( xref )."

sparser
"Recently, a missense mutation in the low density lipoprotein receptor related protein 6 (LRP6) gene, encoding low density lipoprotein receptor related protein 6, has been implicated in an autosomal dominant form of early-onset CAD."

reach
"We found that the anti-aggregation effect of ROCK inhibitors was not limited to the mutant htt and AR and that Y-27632 was also able to reduce the aggregation of ataxin-3 and atrophin-1 with expanded polyQ."

reach
"Y-27632, an inhibitor of Rho kinases, also decreased soluble and insoluble expanded ATXN3 in cells."