IndraLab
Statements
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sparser
"Both AMSH and Usp8 bind components of the endosomal sorting complex for transport (ESCRT) which are involved in targeting ubiquitinated cargo receptors for incorporation into intraluminal vesicles (IL[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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sparser
"The interaction of the two endosomal DUBs, AMSH, and USP8 (UBPY in yeast), with a UIM-containing signal transducing adaptor molecule 2 (STAM2) enhances their deubiquitinating efficacy via substrate arrest [ xref , xref ]."
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reach
"The DUBs AMSH (associated molecule with the SH3 domain of STAM) XREF_BIBR and UBPY (ubiquitin isopeptidase Y/ubiquitin specific protease 8) XREF_BIBR, bind both the ESCRT-0 component STAM (signal transducing adaptor molecule) and ESCRT and III complex members XREF_BIBR, and are implicated in regulating EGFR sorting onto the degradative pathway."
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sparser
"AMSH and USP8 can also interact with CHMP proteins that are components of the late ESCRT-III machinery xref , xref ."
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reach
"The competitive binding of AMSH and USP8 to ESCRT subunits, and the fact that they associate with both ESCRT-0 and -III, suggests that these DUBs may regulate cargo deubiquitination in complex ways."
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sparser
"AMSH and Usp8 bind with their PxxP motif to the non-canonical SH3-binding motif of ESCRT-0 protein STAM, and with their MIT (Microtubule Interacting and Transport)-domain to several CHMP proteins of t[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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sparser
"The competitive binding of AMSH and USP8 to ESCRT subunits, and the fact that they associate with both ESCRT-0 and –III, suggests that these DUBs may regulate cargo deubiquitination in complex ways."
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sparser
"The molecular mechanism underlying these opposing effects is hinted at by the observation that USP8 and AMSH bind a central SH3 domain of STAM proteins in a mutually exclusive manner ( xref )."
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sparser
"The functional relevance of the interaction between USP8, AMSH, and STAM in the endocytic trafficking of activated growth factors is supported by other observations, including that catalytically inactive AMSH, a potential “substrate trap” mutant, resulted in the accumulation of ubiquitin on endosomes, an increased association with STAM, and the generation of a minor product consistent with ubiquitylated STAM ( xref )."
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sparser
"This also contains the sequence PPPRPTAPKP, which binds the Mona SH3 domain [ 39 ] and closely resembles the PXXXRXXKP motifs in UBPY [ 21, 28 ] and AMSH [ 20 ] that bind STAM SH3."
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sparser
"AMSH (a JAMM family member with specificity for K63 chains) and USP8 associate with STAM proteins, a component of the ESCRT-0 complex xref , xref ."
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sparser
"Both AMSH and Usp8 bind to ESCRT-0 protein STAM through a non-canonical SH3-binding motif, and with their MIT-domain to several CHMP proteins of the ESCRT-III machinery [30,37,39,40] ."
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sparser
"Ubiquitylation is a highly dynamic process, and Ub is ultimately removed from cargoes prior to MVB vesicle incorporation by the deubiquitylating ESCRT enzymes, UBPY or AMSH, which interact with both the HRS:STAM adaptor and ESCRT-III subunits ( xref , xref )."
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sparser
"UBPY and AMSH interact with the Hrs-STAM complex and counteract ubiquitination of RTKs."
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sparser
"Like Doa4 in yeast, mammalian Usp8 and AMSH associate with class E Vps proteins on endosomes."
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sparser
"CHMP1 (Did2), CHMP2 (Vps2) and CHMP3 (Vps24) can interact with two mammalian deubiquitinating enzymes, AMSH and UBPY, to facilitate their recruitment directly [ xref , xref , xref ]."
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sparser
"To test this idea, we assayed whether the CHMP4C peptide could competitively inhibit AMSH, MITD1 and USP8 MIT binding to labeled CHMP4B ( xref )."