IndraLab

Statements

PEBP1 binds RAF1. 10 / 210
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"In addition to confirming that locostatin prevents association of RKIP with Raf-1 under conditions where RKIP is preincubated with locostatin, we demonstrate in this report that locostatin added at the same time as RKIP to a Raf-1 sample results in disruption of binding of RKIP to Raf-1 when the sample is incubated at 37degreesC for 6 h, but not at 4degreesC for 30 min (XREF_FIG)."
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"RKIP binds to Raf-1 making an RKIP and Raf complex."
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"Normally, PEBP1 binds to and inactivates Raf-1 (an activator of extracellular-signal-regulated kinase (ERK) pathways), thereby serving as an endogenous inhibitor of those pathways."
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"As shown inFigures xref and xref , I/R-induced phosphorylation of PEBP1 at Ser153 inhibited the interaction between PEBP1 and Raf-1 neurons."
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"I/R Increased the Protein Level and the Phosphorylation of PEBP1 at ser153 and Inhibited the Interaction between PEBP1 and Raf-1 in Neurons."
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"RKIP can associate with Raf-1, thus suppressing the downstream mitogen-activated protein kinase (MAPK) cascade."
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"The unfolding inhibited binding of RKIP to Raf-1 but promoted its binding to GPCR-kinase 2."
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"RKIP negatively regulates Raf-MEK-ERK by binding to Raf1, and also regulates the NFkappaB and G protein coupled receptor signalling pathways depending on its phosphorylation state."
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"The disruption interactions between PEBP1 and Raf1 kinase liberates the protein and makes it available for binding 15LO to promote ferroptosis, which, in turn, can be blocked by GPX4 overexpression and ferrostatin-1 treatment ( xref ) [ xref ]."
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"Additionally, ligand binding blocks RKIP association with Raf-1."
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MEK binds PEBP1 and RAF1. 10 / 12
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"As stated earlier, RKIP can bind to Raf-1 or MEK, yet not at the same time, and binding to either one is enough to cause downstream inhibition [28] ."
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"The binding of RKIP to both Raf-1 and MEK inhibits their phosphorylation and activation, thus leading to downstream suppression of the Raf-1-induced signaling and activity of AP-1-dependent transcription [ xref ]."
| PMC
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"RKIP directly interacts with both RAF1 and MEK, and disrupts the RAF1/MEK interaction, thereby preventing the activation of MEK and downstream components of the signaling cascade."
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"Furthermore, it was shown that Raf-1 and RKIP bind to different sites on MEK."
| PMC
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"Interestingly, it was further shown that MEK and Raf-1 bind to the same sites on RKIP, even though MEK and RKIP are associated with different domains on Raf-1."
| PMC
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"RKIP binds Raf-1 and MEK [81] ."
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"As stated earlier, RKIP can bind to Raf-1 or MEK, yet not at the same time, and binding to either one is enough to cause downstream inhibition. xref In addition, it was postulated that RKIP may be involved in growth, transformation, and differentiation, xref which are often deregulated in many forms of cancer."
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"Mechanistically, RKIP binds both CRAF and MEK."
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"RKIP binds to Raf-1 and MEK, and more weakly to ERK."
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"More specifically, Raf-1 is bound by RKIP at the phosphorylated N-terminal, which blocks the binding of MEK [ xref ]."
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MAP2K1 binds PEBP1 and RAF1. 4 / 4
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"It has been shown that RKIP binds to Raf-1 and MEK1 at overlapping sites [14] ."
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"A yeast two-hybrid assay screen of a human T cell library was used to identify proteins that bound to Raf-1 kinase binding domains. xref The protein identified was designated RKIP, whose sequence was analogous to the human and monkey 23 kDa protein PEBP. xref Using anti-RKIP antibody, antisense RKIP and sense RKIP expression vectors, Yeung et al. discovered that RKIP could bind Raf-1 and MEK-1, and weakly bind to ERK-2, interfering with MEK phosphorylation and activation by Raf-1."
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"RKIP binds to Raf1 and MEK1, and prevents Raf1 in activating MEK [ xref ]."
| PMC
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"In the presence of RKIP, RAF1 cannot bind MEK1 ( xref )."
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ERK binds MEK, PEBP1, and RAF1. 3 / 3
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"On the other hand, unphosphorylated RKIP may bind MEK and ERK, causing Raf-1-MEK dissociation and ERK signaling mitigation."
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"RKIP can form ternary complexes with Raf-1, MEK, and ERK."
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"In vitro, RKIP binds to Raf-1, MEK and ERK, but not to Ras."
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GRK2 binds PEBP1 and RAF1. 3 / 3
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"However, we reported that in addition to GRK2 and Raf-1 binding to PEBP1, the eicosanoid generating enzyme, 15LO1, also binds PEBP1. xref 15LO1, a lipoxygenase that generates 15 hydroxyeicosatetraenoic acid (15s-HETE), is upregulated in response to IL-13 stimulation of HAECs and is increased in asthma in relation to Type-2/eosinophilic processes. xref xref – xref , xref In addition to upregulation of 15LO1 in response to IL-13, stimulation of HAECs with IL-13 promotes binding of 15LO1 to PEBP1, with subsequent release of Raf-1, activation of ERK and increased expression of the airway mucin, MUC5AC. xref Thus, we hypothesized that in the presence of IL-13, 15LO1 would compete with GRK2 for binding to PEBP1, leading to more free GRK2 and greater likelihood for β2AR desensitization (even in the absence of β2 stimulation)."
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"The phosphorylated RKIP interacts with GRK2 instead of RAF1, disrupting the function of GRK2, so it cannot inhibit the GPCR signaling pathway, allowing the signaling to continue and blocking the internalization of the receptor [ xref , xref ]."
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"Under conditions that reflect the kinetics of locostatin’s binding to RKIP and are not unduly stringent, locostatin abrogates the interaction of RKIP with Raf-1 and GRK2 ( xref and xref , and Ref. xref )."
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IKK_complex binds PEBP1 and RAF1. 2 / 2
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"A high level of PEBP1 protein interacts with both Raf1 and IKK to inhibit the NF‐κB signaling cascade, resulting in the sequestration of p65/p50 heterodimer in the cytoplasm, thereby silencing HIV‐1 transcription."
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"Our data also supported that PEBP1 formed a protein complex with Raf1 and/or IKK to suppress NF‐κB signaling and induce HIV latency in CD4 + T cells."
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MAPK binds MAP2K1, PEBP1, and RAF1. 2 / 2
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"This mode of action bears striking similarities to the interaction of RKIP with Raf-1 and MEK1 in the MAPK pathway."
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"This mode of action bears striking similarities to the interactions of RKIP with Raf-1 and MEK1 in the MAPK pathway."
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SNAI1 binds PEBP1, RAF1, and frvX. 1 / 1
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"RKIP associates with Raf-1 and inhibits the Raf/MEK/ERK/Myc signaling, leading to the inhibition of Snail and TET activity and decreased pro-metastatic gene expression[ xref ]."
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PEBP1 binds RAF1 and Nodal. 1 / 1
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"Therefore, it is not that surprising that increased Raf-1 kinase activity by loss of RKIP expression was associated with increased nodal and distant metastases in pancreatic ductal adenocarcinoma specimens and was independent predictor of worsening disease-free survival of pancreatic ductal adenocarcinoma patients ( xref )."
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"Moreover, we found that RKIP interacted with TAK1, NIK, IKK, and Raf-1 and negatively regulated the NF-κB and ERK pathways."
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MAPK1 binds PEBP1 and RAF1. 1 / 1
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IKBKB binds PEBP1 and RAF1. 1 / 1
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"It has been reported that PEBP1 forms a protein complex with Raf1 or IKKβ (Yeung et al , xref ; Granovsky et al , xref )."
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PEBP1 binds PEBP1 and RAF1. 1 / 1
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"These results are consistent with an interaction between the inhibitor RKIP and Raf-1 during early mitosis that is disrupted upon phosphorylation of RKIP, dissociation of pRKIP, and subsequent activat[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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