A database built with INDRA combining content from numerous readers and databases. This page allows you to curate the loaded statements. For more information please see the manual.

IndraLab

Statements

databases
phosphosite cbn pc11 biopax bel_lc signor biogrid tas lincs_drug hprd trrust | geneways tees isi trips rlimsp medscan sparser reach
reading

GRB2 affects EGFR
6 2 51 12 | 7 32 374 141
3 2 51 10 | 5 32 199 126
sparser
"Proline-rich motifs in the N-terminus of RhoU mediate its interaction with GRB2 and the EGFR."
sparser
"For the TIR-FRET screening of larger cell collectives, we performed three separate steps: (1) setting up of a membrane associated test system for probing the interaction between the epidermal growth factor receptor (EGFR) and the growth factor receptor-bound protein 2; (2) use of the Epac-SH188 sensor for quantitative evaluation under the microscope; and (3) application of a TIR fluorescence reader to probe the interaction of GFP with Nile Red."
reach
"Receptor clusters of 100 increase the efficiency of Grb2 rebinding to a second EGFR by 6 fold, compared to randomly distributed receptors at this normal expression level."
sparser
"For example, the GRB2 adaptor protein binds activated EGFR at phosphorylated Tyr1068 xref , and phosphorylated Tyr1173 provides a docking site for the Shc scaffold protein, which is involved in mitogen-activated protein kinase (MAPK) signaling activation xref ."
reach
"It has been shown that EGF activated EGFR interacts with Grb2 at the plasma membrane and in endosomes XREF_BIBR, XREF_BIBR."
biogrid
No evidence text available
reach
"For example, activated EGFR binds to Shc and Grb2, which recruits Sos to the plasma membrane to activate Ras."
sparser
"We also demonstrated the ability of the peptides to block the binding of the GRB2 SH2 domain to EGFR in a mammalian cell-based binding assay."
sparser
"Additionally, the PLA to detect EGFR-GRB2 interaction was evaluated as a prognostic marker in two cohorts of patients with lung adenocarcinoma."
reach
"The underlying mechanism seems to involve recruitment of a Grb2 c and Cbl complex to Grb2 specific docking sites of EGFR, and concurrent acceleration of receptor ubiquitylation and desensitization."
CBL binds EGFR and GRB2. 10 / 31
| 29 2
sparser
"Binding of Grb2 and c-Cbl by EGFR is essential for infection."
sparser
"Residual ubiquitination was further consistent with the reported findings that Cbl can also interact indirectly with the EGFR through Grb2 ."
sparser
"However, it has no effect on the indirect binding of c-Cbl to EGFR via Grb2."
sparser
"On the other hand, the binding of Cbl to EGFR via Grb2 is important for receptor internalization [ xref ]."
sparser
"Here we use a simple modelling approach together with experiments to show that the establishment of the threshold requires both the multiplicity of binding sites and cooperative binding of Cbl and Grb2 to the EGFR."
sparser
"Further, only indirect binding of Cbl to the EGFR through Grb2 is considered important for recruitment of the EGFR to clathrin-coated pits and thus essential for ligand-induced endocytosis of the EGF[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"In cell culture studies, the indirect binding of EGFR to Cbl through Grb2 is necessary for receptor internalization, whereas endosomal sorting requires direct binding to Cbl xref , xref ."
sparser
"In addition, Cbl can bind indirectly to the EGFR via Grb2 [ xref ]."
sparser
"Additionally, Cbl can also indirectly bind EGFR through the Grb2 adaptor protein at the Y1068 and Y1086 sites of the receptor xref ."
sparser
"Like c-Cbl, Grb2 bound EGFR more efficiently following EGF or BTC stimulation for 15 min compared with the other EGFR ligands ( xref )."
SHC binds EGFR and GRB2. 10 / 23
| 23
sparser
"An earlier study has shown that Grb2 can interact with EGFR indirectly through Shc ( xref ; xref ), enabling the SH2 domain of Grb2 to interact with other proteins."
sparser
"Notably, activated EGFR interacts with Shc and Grb2 not only at the plasma membrane but also in the endosomal compartment, where EGF induces augmented recruitment of Shc and Grb2."
sparser
"We first describe our strategy to inhibit Grb2/Sos interaction at the level of its SH3 domains and then report the results obtained in blocking Grb2 SH2 domain interaction with EGF-R or Shc."
sparser
"Sem-5 is homologous to the mammalian protein Grb2, which binds the autophosphorylated EGF receptor and other phosphotyrosine-containing proteins such as Shc through its SH2 domain."
sparser
"The phosphorylation of these receptors, such as EGFR, also induces the tyrosine phosphorylation of the adapter protein SHC and the association of SHC with Grb2."
sparser
"EGFR binding and phosphorylation of Shc and Grb2 initiate this pathway, either by association directly with Grb2, or with a Shc-Grb2 complex (Rozakis-Adcock et al., 1992; Batzer et al., 1994)."
sparser
"The Src kinase, Yes, is activated by CCK and mediates EGFR Shc-Grb2 interactions ( xref )."
sparser
"The concentrations of EGF for which PI 3-kinase inhibitors block Ras activation induce formation of Shc-Grb2 complexes but not detectable EGF receptor phosphorylation and do not activate PI 3-kinase."
sparser
"Surprisingly, EGF and TGF-alpha induced full activation of MAPK in duration and intensity and stimulated association of the EGFR with Shc and Grb2."
sparser
"By contrast, Shc and Grb2 were inducibly associated with the EGFR in response to EGF stimulation of glioma cells."
SOS binds EGFR and GRB2. 10 / 17
| 17
sparser
"Upon EGFstimulation of the cell, binding of Sos to the EGF receptor-Grb2 complex brings a significant portion of Sos to the membrane (Figure 5)."
sparser
"EGF treatment of cells promotes the formation of a complex of EGF receptor, Grb2, and Sos."
sparser
"The measurable catalytic exchange activity of Sos in immunocomplex is unaltered by the association of Sos with EGF receptor and Grb2, either when the association occurs in EGF- stimulated cells or by [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"In contrast to the activated epidermal growth factor receptor that binds the GRB2-Sos complex directly, activation of the insulin receptor results in the interaction of GRB2-Sos with IRS-1 and Shc, thus linking the insulin receptor to Ras signaling pathways."
sparser
"As expected, mutation of the Grb2 binding site significantly reduced the interaction of Grb2 and SOS with EGFR ( xref )."
sparser
"We only considered this effect for the assembly of the EGFR-Grb2-Sos complex, but other components of the EGRF signaling network may also decrease their mobility upon activation."
sparser
"Zhang used this system to demonstrate the Grb2-mediated interaction of the cytoplasmic domain of the epidermal growth factor receptor with the Sos protein."
sparser
"Following EGFR activation, Grb2 and Sos (adaptor proteins) bind EGFR directly at Y1092 and Y1110 or through Shc (which binds at EGFR Y1172 and Y1197) [ xref ]."
sparser
"If the stimulation of exchange activity measured in this permeabilized cell system was due to the formation of EGF receptor-Grb2-Sos complexes, then it may be possible to inhibit it by the use of t[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"In response to epidermal growth factor (EGF), the activated EGF receptor binds to a complex containing the signaling protein GRB2 and the Ras guanine nucleotide-releasing factor Sos, leading to activation of the Ras signaling pathway."
EGF binds EGFR and GRB2. 10 / 16
2 | 14
sparser
"Upon EGF stimulation, Grb2 binds phosphorylated EGFR and activates the signaling pathway xref , xref ."
sparser
"Together these data indicate that AG-1478 inhibits both constitutive as well as EGF-induced EGFR-Grb2 interaction."
sparser
"Preclinical studies revealed cetuximab could decrease cell proliferation and phosphorylation of EGFR, and blocked the binding of the adaptor protein Grb2 to EGFR upon activation by EGF [ xref ]."
sparser
"Interestingly, in spite of the free phosphonate group with its negative charges, CGP78850 inhibited association of Grb2 with EGFR in intact serum-starved MDA-MB-468 triple-negative breast cancer cells stimulated with EGF in vitro ."
sparser
"Our results support the concept that EGFR interacts with Grb2 in both constitutive and EGF-dependent manners and this interaction is independent of HER3 co-expression."
sparser
"1) Association of Grb2 to EGFR in BxPC-3 induced by EGF in the presence of Erbitux indicates an alternate pathway of Ras-MAPK activation, which may be related with the tumor resistance to treatment; 2) transactivation of EGFR downstream Ras-MAPK pathway by FGF contributes the resistance to treatment; and 3) downregulation of EGFR may increase the response to therapy."
sparser
"Compound inhibited epidermal growth factor (EGF)-induced protein–protein interactions between EGFR (EGF receptor) and Grb2 in MDA-MB-468 cells and inhibited Ras activation in the same cell line."
sparser
"We identified that As 2 O 3 activates EGFR and promotes phosphorylation of p66 ShcA and its interaction with the Grb2 adaptor protein with slower kinetics compared to EGF-mediated EGFR activation."
sparser
"Because Ras activation by EGF involves EGF-stimulated association of EGF-R with Grb2, the EGF-R was immunoprecipitated and a Western blot was probed for Grb2."
sparser
"Pre-clinical studies showed cetuximab decreased cell proliferation and phosphorylation of EGFR, and blocked the binding of the adaptor protein Grb2 to EGFR upon activation by EGF [ xref ]."
LRRK1 binds EGFR and GRB2. 7 / 7
| 6 1
sparser
"We recently showed that LRRK1 forms a complex with activated EGFR through a mutual interaction with Grb2 ( xref )."
sparser
"Our results demonstrate that LRRK1 forms a complex with EGFR via Grb2 and associates with STAM and Hrs in response to ligand stimulation."
sparser
"LRRK1 forms a complex with activated EGFR via Grb2."
reach
"Interestingly, LRRK1 has been proposed to regulate endocytosis of EGFR via formation of a LRRK1, Grb2, and EGFR complex, while LRRK2 did not appear to form such a complex in this study suggesting that the two have functionally diverged."
sparser
"Interestingly, LRRK1 has been proposed to regulate endocytosis of EGFR via formation of a LRRK1-Grb2-EGFR complex ( xref ), while LRRK2 did not appear to form such a complex in this study suggesting that the two have functionally diverged."
sparser
"In this paper, we demonstrate that leucine-rich repeat kinase LRRK1, which contains a MAPKKK-like kinase domain, forms a complex with activated EGFR through an interaction with Grb2."
sparser
"We previously reported that LRRK1 forms a complex with activated EGFR through an interaction with Grb2 ( xref )."
SHC binds SOS, EGFR, and GRB2. 4 / 4
| 4
sparser
"HO also induces the association of the Shc-Grb2-Sos complex with the EGF receptor, resulting in activation of downstream signalling systems ."
sparser
"Once the SHC-Grb2-SOS complex is associated with the EGFR, the SOS promotes the exchange of GDP for GTP on Ras, a small G protein."
sparser
"After EGFR activation, a trimeric complex between tyrosine phosphorylated Shc, Grb2, and Sos is formed and this, in turn, triggers downstream mitogenic signaling xref ."
sparser
"The Grb2-SOS complexes that have bound to EGFR or the EGFR-Shc complex catalyse the conversion of RasGDP to RasGTP (reaction 28), whereas the reverse transition is catalysed by RasGAP bound to the RP 2 form of EGFR (reaction 29)."
Tyrosine-phosphorylated EGFR binds GRB2. 3 / 3
| 2 1
trips
"These analyses reveal a hierarchy of interactions between Grb2 and Shc with the EGFR and indicate that Grb2 can bind the tyrosine-phosphorylated EGFR directly, as well as indirectly via Shc."
trips
"Immunoblotting experiments indicate that GRB2 associates with tyrosine-phosphorylated epidermal growth factor receptors (EGFRs) and platelet-derived growth factor receptors (PDGFRs) via its SH2 domain."
reach
"In support of this model, Gab2 mutants defective in 14-3-3 binding exhibit increased recruitment of Grb2 and consequently sustained association with the tyrosine phosphorylated EGFR and Shc."
Phosphorylated EGFR binds GRB2. 3 / 3
| 3
reach
"Upon EGF stimulation, Grb2 binds phosphorylated EGFR and activates the signaling pathway XREF_BIBR, XREF_BIBR."
reach
"One model to emerge is that Grb2, when bound to phosphorylated EGFR, recruits c-Cbl for the purpose of degrading the receptor and terminating RTK signaling in a classic negative-feedback loop."
reach
"Although phosphorylated EGFR can bind to both Shc and Grb2, the predominant linkage was observed between EGFR and Shc."
SOS1 binds EGFR and GRB2. 3 / 3
1 | 2
bel
"In response to stimulation with epidermal growth factor (EGF), the guanine nucleotide exchange factor human SOS1 (hSOS1) promotes the activation of Ras by forming a complex with Grb2 and the human EGF receptor (hEGFR)"
sparser
"Upon EGF stimulation, SOS1 binds to phosphorylated EGFR through the adaptor protein GRB2, which then triggers RAS activation at the plasma membrane ."
sparser
"Although the low-copy-number vector pBT(L) was the best in the case of EGFR-Grb2-Sos1 Y3H interaction, it may also cause reduced bait expression and lowered sensitivity for the detection of weaker protein interactions."
SHC1 binds EGFR and GRB2. 3 / 3
1 | 2
hprd
No evidence text available
sparser
"3.1 As 2 O 3 Induces EGFR and ShcA Phosphorylation and Interaction with Grb2 Adaptor Proteins in HEY Ovarian Carcinoma Cells."
sparser
"It has been reported that after administration of a single receptor-saturating dose of EGF there is a rapid and extensive receptor internalisation with a concomitant tyrosine phosphorylation of 170- a[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
EGFR binds GRB2 and MET. 2 / 2
| 1 1
reach
"On activation of certain RTKs such as EGFR and MET, Gab1 is recruited to the plasma membrane via interaction with EGFR bound Grb2 or directly with MET, followed by the phosphorylation of a number of tyrosine residues on Gab1."
sparser
"Prior co-immunoprecipitation experiments provided indirect evidence that Grb2 is the primary target of 1 and that 1 was able to disrupt Grb2-EGFR and Grb2-c-Met interactions. xref , xref "
ERBB binds EGFR and GRB2. 2 / 2
| 2
sparser
"For example, the SH2 domain of Grb2 and others bind to the phosphorylated Tyr1068 residues of EGFR and ErbB family members at other residues xref ."
sparser
"For example, the SH2 domain of Grb2 and others bind to the phosphorylated Tyr1068 residues of EGFR and ErbB family members at other residues [50] ."
EGFR binds GRB2 and PTK2B. 2 / 2
| 2
sparser
"This translocation is accomplished by activation of protein tyrosine kinases, such as Src family kinases, Bruton’s tyrosine kinase, Pyk2, epidermal growth factor (EGF) or platelet-derived growth facto[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"As Grb2 association with scaffolds, such as EGFR or Pyk2, is vital for their capacity to activate ERK1/2 cascades, we investigated the relative effects of R1- versus R4-level peroxide exposures upon Grb2 interaction with EGFR or Pyk2."
EGFR binds GRB2 and pelA. 2 / 2
| 2
sparser
"In two series of lung adenocarcinoma patients, PLA EGFR:GRB2 was independently associated with longer survival (HR=0.46, CI 95% 0.2–0.78, p=0.0085 and HR=0.48, CI 95% 0.2–0.85, p=0.017)."
sparser
"Low EGFR:GRB2 PLA group (1 st quartile) was independently associated with worse survival in both Yale cohorts A and B. Gender and stage were shown to be independent prognostic markers only in Yale cohort A."
EGFR binds GRB2 and p66shc. 2 / 2
| 2
sparser
"Indeed, during severe oxidative stress, increased binding of p66shc to the activated EGFR and Grb2 occurs."
sparser
"However, during severe oxidative stress, increased binding of p66shc to the activated EGFR and Grb2 occurs."
EGFR binds GRB2 and yfiA. 2 / 2
| 2
sparser
"However, we observed increased Grb2 binding to pY-EGFR (for both EGF and As 2 O 3 ) ( xref )."
sparser
"Upon ligand binding to ERBB1, also known as EGFR, the E3-ubiquitin ligase Cbl is recruited directly to EGFR phosphotyrosine 1045 (pY1045) or indirectly through Grb2, which binds EGFR pY1068 and pY1086[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
EGFR binds GAB1 and GRB2. 2 / 2
| 2
sparser
"It is well established that Gab1 binds directly and specifically to Met , and strong evidence indicates that Gab1 binds EGFR indirectly via Grb2 , although direct binding has also been suggested ."
sparser
"Gab1 interacts with EGFR via constitutively bound Grb2, becomes phosphorylated and binds PI3-K, resulting in its activation, consequently, enhancement of PIP3 level in the cell membrane."
GST binds EGFR and GRB2. 2 / 2
| 2
sparser
"To determine whether complement-induced EGF-R tyrosine phosphorylation correlated with EGF-R activation, we examined if EGF-R interacted with a GST-Grb2 fusion protein."
sparser
"Support for this conclusion was provided by experiments showing that GST-Grb2 fusion protein bound the complement-transactivated EGF-R (), and that complement-dependent ERK2 and cPLA activation were b[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
EGFR binds GRB2 and PLCG1. 2 / 2
| 2
sparser
"Furthermore, PLC-γ1 Y783F as well as PLC-γ1 WT specifically interacts with EGFR, suggesting that EGFR may not involve in the interaction between PLC-γ1 and Grb2."
sparser
"To elucidate whether the tyrosine phosphorylated PLC-γ1 indirectly interacts with Grb2 through EGFR, we generated tyrosine phosphorylation-defective mutants in which tyrosine residues 771, 783 and 125[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
SHC binds EGFR, GRB2, and IRS1. 2 / 2
| 2
sparser
"Furthermore, we have identified a short sequence motif (YV/IN) present in IRS-1, EGFR and Shc, which specifically binds the SH2 domain of GRB2 with high affinity."
sparser
"24 GRB2 over-expression is particularly interesting in breast cancer because GRB2 can bind to tyrosine-phosphorylated EGFR, SHC, insulin receptor substrate-1, and HER2/neu,1'15'26'27 which have all be[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
SRC binds BLNK, EGFR, and GRB2. 2 / 2
| 2
sparser
"The Src homology 2 (SH2) domain of Grb2 binds to autophosphorylation sites of EGFR, including Y1068, and several other receptors."
sparser
"Phosphorylated EGFR binds the Src homology 2 (SH2) domain of the adaptor protein GRB2, which in turn recruits the guanine nucleotide exchange factor SOS."
EGFR binds GRB2, CFP, and luxY. 2 / 2
| 2
sparser
"In particular, FRET measurements indicated that activated EGFR-CFP interacted with Grb2-YFP in membrane ruffles and endosomes."
sparser
"Previously, Sorkin et al. xref used FRET microscopy to image the association of EGFRCFP with Grb2YFP in porcine aortic cells using filter-based fluorescence imaging."
SHC binds EGF, EGFR, and GRB2. 2 / 2
| 2
sparser
"Dominant-negative forms of SHP-2 had no effect on EGF-stimulated interaction of GRB2 with EGFR or SHC, nor did they influence phosphorylation of SHC and SHC/EGFR association."
sparser
"Experiments with rat liver have demonstrated that following the administration of a bolus of EGF, intracellular EGFR are associated with Shc, Grb2, and mSOS [73] ."
sparser
"Stimulation with ufCB also induced processing of membrane-anchored proheparin-binding (HB)-EGF, release of soluble HB-EGF into the medium, association of phosphorylated EGF-R and Shc with glutathione-S-transferase-Grb2 fusion protein, and phosphorylation of extracellular signal-regulated kinase (ERK)."
sparser
"ET-1 rapidly increased association of EGFR and Shc with glutathione-S-transferase-Grb2 fusion protein."
| 2
sparser
"The role of EGFR interaction with the Grb2 adaptor protein and Cbl ubiquitin ligase, and receptor ubiquitination in the clathrin-dependent internalization and sorting of EGFR in multivesicular endosomes is discussed."
sparser
"The role of EGFR interaction with the Grb2 adaptor protein and Cbl ubiquitin ligase, and receptor ubiquitination in the clathrin-dependent internalization and sorting of EGFR in multivesicular endosomes is discussed."
RAF binds RAS, SOS, EGFR, and GRB2. 2 / 2
| 2
sparser
"The EGFR interacts with adaptor proteins Grb2 and SOS, activating Ras and Raf, followed by phosphorylation of Mitogen-activated protein kinase/ERK and extracellular signal–regulated kinase (ERK)."
sparser
"Overexpression and/or mutant forms of EGFR, GRB-2, SOS, RAS and RAF are reported to cause oncogenic transformation (Bi- shop, 1991; McCormick, 1993; Cuadrado et al., 1993; Egan and Weinberg, 199[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
CISH binds EGFR and GRB2. 1 / 1
| 1
sparser
"In mammalian cells, Grb2 can interact with negative regulators of EGFR signaling such as the suppressor of cytokine signaling (SOCS) and the SH2 domain inositol 5-phosphatases (SHIPs) [23,42] ."
TOM1L1 binds EGFR and GRB2. 1 / 1
| 1
sparser
"Shc may thus mediate interaction of Tom1L1Grb2 complex with EGFR."
Phosphorylated EGFR binds GRB2 and SH2 domains. 1 / 1
| 1
reach
"Cbl proteins are recruited to the activated EGFR either by the direct binding of their TKB domain to the phospho-tyrosine residue at position 1045 in the EGFR, or by an indirect mechanism mediated by Grb2, as the SH3 domains of Grb2 bind the proline rich region of Cbl proteins and the SH2 domains of Grb2 binds the phosphorylated EGFR."
PDGF binds EGFR and GRB2. 1 / 1
| 1
sparser
"Although GRB2 binds to the EGF receptor and the PDGF #-receptor, it does not become tyrosine phosphorylated in response to 48 L. Claesson-Welsh ligand stimulation."
RHOU binds EGFR and GRB2. 1 / 1
| 1
sparser
"Moreover, RhoU physically associates with activated EGFR in a GRB2-dependent manner through specific proline-rich motifs within its N-terminus."
EGFR binds GRB2 and SH. 1 / 1
| 1
sparser
"After its autophosphorylation of EGFR, it binds the adaptor protein Grb2 through Grb2 SH-2 domain, and activates downstream effectors including Ras, Raf-1, MAPK, etc, in turn."
EGFR binds GRB2 and PTPN1. 1 / 1
| 1
sparser
"Protein phosphatase PTP1B binds to EGFR through Grb2 adaptor protein to terminate EGFR signaling and protein phosphatase MKP2 terminates the Erk1/2 signaling."
EGFR binds GRB2 and glaH. 1 / 1
| 1
reach
"The PH domain mediated recruitment of Gab proteins provides the opportunity to modulate their membrane residency by PI3K and lipid phosphatases in a very dynamic manner without the need to disrupt large signalosomes such as the EGFR, Grb2, and Gab complex that are potentially stabilised by various direct and indirect protein protein interactions."
WAS binds EGFR and GRB2. 1 / 1
| 1
sparser
"She H-Y, Rockow S, Tang J, Nishimura R, Skolink EY, Chen M, Margolis B, Li W: Wiskott-Aldrich syndrome protein is associated with the adapter protein Grb2 and the epidermal growth factor recepto[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
Phosphorylated EGFR binds GRB2 and (SH2) domains. 1 / 1
| 1
reach
"In this study, we generated fusion proteins containing one or two Src-homology 2 (SH2) domains of growth factor receptor bound protein 2 (Grb2), which bind to phosphorylated EGFR, added with HIV-1 transactivating transcription for cell membrane penetration (termed TSF and TSSF, respectively)."
| 1
sparser
"Also, severe oxidative stress (unlike ligand stimulation and moderate oxidative stress, both of which support survival) increased binding of p66(shc) to the activated EGFR and Grb2."
EGFR binds GRB2 and Y1110. 1 / 1
| 1
reach
"Following EGFR activation, Grb2 and Sos (adaptor proteins) bind EGFR directly at Y1092 and Y1110 or through Shc (which binds at EGFR Y1172 and Y1197) [XREF_BIBR]."
SRC binds EGFR and GRB2. 1 / 1
| 1
sparser
"Combined cell lysates were affinity-purified over the SH2 domain of the adapter protein Grb2 (GST-SH2 fusion protein) that specifically binds phosphorylated EGFR and Src homologous and collagen (Shc) protein."
RTK binds EGFR and GRB2. 1 / 1
| 1
sparser
"The internalisation step requires association of EGFR with signalling molecules such as Grb-2 and activity of the receptor tyrosine kinase ; and the tyrosine kinase activity is also required in targe[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
SHC2 binds EGFR and GRB2. 1 / 1
| 1
sparser
"Single molecule pulldowns reveal that an EGFR ligand-stimulated interaction between Shc2 and Grb2 reduces Grb2 availability to bind to the EGFR phospho-sites, leaving these sites available for phospha[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
EGFR binds GRB2 and SH2 domain. 1 / 1
| 1
reach
"These discoveries were extended to mammalian systems, where it was shown that the Grb2 SH2 domain could inducibly bind the tyrosine phosphorylated epidermal growth factor receptor (EGFR) while bound constitutively to son of sevenless (SOS), a guanine nucleotide exchange factor for Ras."
EGFR binds GRB2 and p66Shc. 1 / 1
| 1
sparser
"Increased binding of p66Shc to activated EGFR and Grb2 occurs during severe oxidative stress."
Phosphorylated EGFR binds GRB2 and SH2 domain. 1 / 1
| 1
reach
"This begins with the recruitment of Grb2, which contains an SH2 domain and can bind either directly to the phosphorylated EGFR, or to Shc, an adapter protein that becomes associated with and tyrosine phosphorylated by the EGFR."
SYK binds EGFR and GRB2. 1 / 1
| 1
sparser
"Immunoprecipitation suggested co-association of Syk with epidermal growth factor receptor and GRB-2."
HBEGF binds EGFR and GRB2. 1 / 1
| 1
sparser
"The HB-EGF induced association of Grb2 with EGFR that correlated with pEGFR ( Figure 5 A)."
MAPK binds EGFR and GRB2. 1 / 1
| 1
sparser
"Phosphorylation site 1068 of EGFR forms a complex with Grb2 and increases mitogen-activated protein kinase activation [ xref ]."
PLCG binds EGFR and GRB2. 1 / 1
| 1
sparser
"The association of Grb2 and PLCγ with a 5YF mutant of the EGFR cytoplasmic domain has not previously been reported."
RPS6 binds EGFR and GRB2. 1 / 1
| 1
sparser
"Image 5 Fig. S6 [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
EGFR binds GRB2 and GSTP1. 1 / 1
| 1
sparser
"As shown in Figure xref , GSTP1 and GRB2 could be detected in the immunoprecipitation complex of phospho-EGFR antibody in 30 ng/mL EGF-stimulated CNE2 cells, and could not be detected by the pretreatment of the cells with 1 μm EGFR inhibitor PD153035, which indicates that GSTP1 and GRB2 can interact with phospho-EGFR, are downstream targets of EGFR signaling pathway."
EGFR binds ERBB3 and GRB2. 1 / 1
| 1
sparser
"Importance of the Grb2 binding sites in HER3 for Grb2 interaction with EGFR-HER3 heteromers."
EGFR binds GRB2 and PTPN11. 1 / 1
| 1
sparser
"In Tibetans, three main signaling pathways associated with GRB2, EGFR, and PTPN11 were shown on a network to be significantly downregulated when compared with Han Chinese ( xref )."
SHC binds SOS1, EGFR, and GRB2. 1 / 1
| 1
sparser
"In response to calcium influx, Shc, Grb2, and Sos1 inducibly associate with a 180-kDa tyrosine-phosphorylated protein, which was determined to be the epidermal growth factor receptor (EGFR)."
SHC binds EGFR, GRB2, and MAP3K1. 1 / 1
| 1
sparser
"Thus, it appears that activation of JNK by EGF may involve a transient association of the Grb2MEKK1 complex with Shc and the EGF receptor, causing a transient plasma membrane localisation of MEKK1 an[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
SHC binds EGFR, GRB2, and COS7. 1 / 1
| 1
sparser
"However, Grb2 and Shc in the mitotic COS7 cells did not bind to activated EGFR."
EGFR binds GRB2, PI3-kinase, and homolog 2. 1 / 1
| 1
sparser
"Using the BioNet tool, a sub-network with EGFR at the core was screened from the PPI network, in which EGFR was associated with v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 ( ERBB2 ), growth factor receptor-bound protein 2 ( GRB2 ) and phosphoinositide-3-kinase, regulatory subunit 1 ( PIK3R1 ) ( xref )."
SHC binds TAC1, EGFR, and GRB2. 1 / 1
| 1
sparser
"SP induced formation of an activated EGFR complex containing the adapter proteins SHC and Grb2, but not c-Src."
ERK binds SHC, EGFR, and GRB2. 1 / 1
| 1
sparser
"In line with our observation of persistence ERK activity, ligand-independent EGFR activation associated with the signaling adaptor proteins, Grb2 and Shc."
PKA binds EGFR, GRB2, and rI. 1 / 1
| 1
sparser
"A prime example is the demonstration that the RI and C subunits of PKA physically interact with the activated EGFR in a Grb2-dependent manner [ xref ]."
RAS binds SHC, EGFR, and GRB2. 1 / 1
| 1
sparser
"We also show that the truncated EGF receptor constitutively associates with the adapter proteins Shc and Grb2 which are involved in the recruitment of Ras to activated receptors."
SHC1 binds EGFR, GRB2, and PLEC. 1 / 1
1 |
hprd
No evidence text available
SHC1 binds EGFR, GRB2, and Sos. 1 / 1
| 1
sparser
"Stimulation of EDNRB in Müller cells resulted in phosphorylation of tyrosine residue 1173 of the EGFR, which is one of the major autophosphorylation site that allows binding of GRB2, SOS and SHC adapter proteins to EGFR and that mediates Ras-Raf activated MAPK/ERK signaling [ xref ]."
PPP2 binds SHC, EGFR, and GRB2. 1 / 1
| 1
sparser
"Consistent with this observation, H(2)O(2) stimulated EGFR tyrosine phosphorylation and complex formation with Shc-Grb2 that was abolished by PP2, implicating Src in H(2)O(2)-induced EGFR activation."
RAS binds EGF, EGFR, and GRB2. 1 / 1
| 1
sparser
"This effect has been associated with reduced EGF-induced EGF receptor (EGFR) tyrosine phosphorylation, Grb-2 association, and Ras activation."
CBL binds EGF, EGFR, and GRB2. 1 / 1
| 1
sparser
"Consistent with the autophosphorylation data, the EGF-induced interaction of c-Cbl and Grb2 proteins with EGFR was elevated in the controls and lowered in the HER2 cells ( xref ), while the reverse was true for Gab1, p85 (subunit of PI3K) and SHP2."
SHC binds EGFR, ERBB2, and GRB2. 1 / 1
| 1
sparser
"The SH2 domains of Shc and/or GRB2 bind to tyrosine phosphorylated EGFR or erbB2, bringing the Ras guanine nucleotide exchange factor SOS into the complex, and initiating the ras/MAP Kinase pathway ( [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
CBL binds EGFR, GRB2, and JAK1. 1 / 1
| 1
sparser
"Other mechanisms that could account for the effect of JAK1 on EGFR expression may involve the adaptor protein GRB2 and the ubiquitin ligase CBL, both of which interact with JAK1 and EGFR and modulate EGFR expression ( xref ; xref )."
EGFR binds EGFR, GRB2, and PAK1. 1 / 1
| 1
sparser
"This interaction is independent of EGF stimulation, but it is increased after stimulation of the EGF receptor and EGFR-Grb2-PAK1 interaction is required for EGF induced lamellipodia formation."
RTK binds SOS1, EGFR, and GRB2. 1 / 1
| 1
sparser
"In xref , experimental results show that GRB2 recruits SOS1 to the membrane to form GRB2-SOS1 complex upon the activation of EGFR or other RTKs."
SHC binds TOM1L1, EGFR, and GRB2. 1 / 1
| 1
sparser
"These results suggest that EGF triggers a transient Grb2/Shc-mediated association of EGFR with Tyr-phosphorylated Tom1L1 to engage the endocytic machinery for endocytosis of the ligand–receptor complex."
| 1
sparser
"We also noted reduced Grb2-EGFR interaction with p66 ShcA knockdown in the presence of As 2 O 3 implicating p66 ShcA isoform in mediating this interaction."
MAPK binds RAS, SRC, EGFR, and GRB2. 1 / 1
| 1
sparser
"This resulted in increased binding of another adaptor protein Grb2 to the Src-EGFR complex and the subsequent activation of Ras and MAPKs."
SOS binds SRC, EGFR, GRB2, and JAK3. 1 / 1
| 1
sparser
"This pathway involves interactions with Grb2, Sos and the kinases EGF-R, JAK3 and Src."
SHC binds SRC, BLNK, EGFR, and GRB2. 1 / 1
| 1
sparser
"Also, our data show that EGF receptors are able to phosphorylate SHC, activate the exchange of guanine nucleotide on ras and phosphorylate MAPK by a mechanism that does not require receptor autophosphorylation sites and, therefore, the src homology 2 (SH2):phosphotyrosine-dependent interaction of SHC or GRB2 with the EGF receptor."
SHC binds CPD, EGF, EGFR, and GRB2. 1 / 1
| 1
sparser
"Coimmunoprecipitation studies demonstrated that both EGF and Cpd 5 induced tyrosine phosphorylation of EGFR was associated with increased amounts of adapter proteins Shc and Grb2, and the Ras GTP-GDP exchange protein Sos, indicating the formation of functional EGFR complexes."
SHC binds EGF, EGFR, GRB2, and RasGEF. 1 / 1
| 1
sparser
"As shown in xref , EGF binds the EGF receptor (EGFR) to recruit RasGEFs such as SOS via its SHC domain and Grb2 adapter protein xref , xref , xref , xref ."
PI3K binds SHC, SRC, EGFR, and GRB2. 1 / 1
| 1
sparser
"Using the chimera system, Wang et al [ xref ] identified 4 tyrosine moieties on the MUC1 CT that are phosphorylated upon activation with anti-CD8 antibody as Y20, Y35, Y46, and Y60 which constitute consensus sequences for binding of PI3K, Shc, EGFR or Src, and Grb2, respectively [ xref ]."
EGF binds EGFR, GRB2, eGFP, and mRFP. 1 / 1
| 1
reach
"The amplitude of the autocorrelation function is inversely related to the cluster density of an EGF, EGFR, eGFP, Grb2, and mRFP complex (eq 4)."
| 1
sparser
"As further evidence of BCAR1 coupling to the EGFR pathway, BCAR1 associates with GRB2 and SHC to mediate integrin-mediated adhesion [ xref ] and serum responses [ xref ]."
SHC binds EGFR, GRB2, CFP, and luxY. 1 / 1
| 1
sparser
"Stimulation of cells by EGF resulted in the accumulation of large pools of Grb2-CFP and YFP-Shc in endosomes, where these two adaptor proteins formed a complex with EGFR."
SHC binds SRC, EGFR, AGT, and GRB2. 1 / 1
| 1
sparser
"Furthermore, the association of Src with Shc, Grb2 and the EGFR after angiotensin II stimulation [ xref ], together with previously described Src-mediated phosphorylation of tyrosine residues Y845 and Y1101 of the EGFR [ xref , xref ], suggest an additional means of receptor activation utilized by GPCRs that is distinct from EGFR autophosphorylation."
EGFR affects GRB2
6 2 51 12 | 7 32 374 141
3 2 51 10 | 5 32 199 126
sparser
"Proline-rich motifs in the N-terminus of RhoU mediate its interaction with GRB2 and the EGFR."
sparser
"For the TIR-FRET screening of larger cell collectives, we performed three separate steps: (1) setting up of a membrane associated test system for probing the interaction between the epidermal growth factor receptor (EGFR) and the growth factor receptor-bound protein 2; (2) use of the Epac-SH188 sensor for quantitative evaluation under the microscope; and (3) application of a TIR fluorescence reader to probe the interaction of GFP with Nile Red."
reach
"Receptor clusters of 100 increase the efficiency of Grb2 rebinding to a second EGFR by 6 fold, compared to randomly distributed receptors at this normal expression level."
sparser
"For example, the GRB2 adaptor protein binds activated EGFR at phosphorylated Tyr1068 xref , and phosphorylated Tyr1173 provides a docking site for the Shc scaffold protein, which is involved in mitogen-activated protein kinase (MAPK) signaling activation xref ."
reach
"It has been shown that EGF activated EGFR interacts with Grb2 at the plasma membrane and in endosomes XREF_BIBR, XREF_BIBR."
biogrid
No evidence text available
reach
"For example, activated EGFR binds to Shc and Grb2, which recruits Sos to the plasma membrane to activate Ras."
sparser
"We also demonstrated the ability of the peptides to block the binding of the GRB2 SH2 domain to EGFR in a mammalian cell-based binding assay."
sparser
"Additionally, the PLA to detect EGFR-GRB2 interaction was evaluated as a prognostic marker in two cohorts of patients with lung adenocarcinoma."
reach
"The underlying mechanism seems to involve recruitment of a Grb2 c and Cbl complex to Grb2 specific docking sites of EGFR, and concurrent acceleration of receptor ubiquitylation and desensitization."
CBL binds EGFR and GRB2. 10 / 31
| 29 2
sparser
"Binding of Grb2 and c-Cbl by EGFR is essential for infection."
sparser
"Residual ubiquitination was further consistent with the reported findings that Cbl can also interact indirectly with the EGFR through Grb2 ."
sparser
"However, it has no effect on the indirect binding of c-Cbl to EGFR via Grb2."
sparser
"On the other hand, the binding of Cbl to EGFR via Grb2 is important for receptor internalization [ xref ]."
sparser
"Here we use a simple modelling approach together with experiments to show that the establishment of the threshold requires both the multiplicity of binding sites and cooperative binding of Cbl and Grb2 to the EGFR."
sparser
"Further, only indirect binding of Cbl to the EGFR through Grb2 is considered important for recruitment of the EGFR to clathrin-coated pits and thus essential for ligand-induced endocytosis of the EGF[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"In cell culture studies, the indirect binding of EGFR to Cbl through Grb2 is necessary for receptor internalization, whereas endosomal sorting requires direct binding to Cbl xref , xref ."
sparser
"In addition, Cbl can bind indirectly to the EGFR via Grb2 [ xref ]."
sparser
"Additionally, Cbl can also indirectly bind EGFR through the Grb2 adaptor protein at the Y1068 and Y1086 sites of the receptor xref ."
sparser
"Like c-Cbl, Grb2 bound EGFR more efficiently following EGF or BTC stimulation for 15 min compared with the other EGFR ligands ( xref )."
SHC binds EGFR and GRB2. 10 / 23
| 23
sparser
"An earlier study has shown that Grb2 can interact with EGFR indirectly through Shc ( xref ; xref ), enabling the SH2 domain of Grb2 to interact with other proteins."
sparser
"Notably, activated EGFR interacts with Shc and Grb2 not only at the plasma membrane but also in the endosomal compartment, where EGF induces augmented recruitment of Shc and Grb2."
sparser
"We first describe our strategy to inhibit Grb2/Sos interaction at the level of its SH3 domains and then report the results obtained in blocking Grb2 SH2 domain interaction with EGF-R or Shc."
sparser
"Sem-5 is homologous to the mammalian protein Grb2, which binds the autophosphorylated EGF receptor and other phosphotyrosine-containing proteins such as Shc through its SH2 domain."
sparser
"The phosphorylation of these receptors, such as EGFR, also induces the tyrosine phosphorylation of the adapter protein SHC and the association of SHC with Grb2."
sparser
"EGFR binding and phosphorylation of Shc and Grb2 initiate this pathway, either by association directly with Grb2, or with a Shc-Grb2 complex (Rozakis-Adcock et al., 1992; Batzer et al., 1994)."
sparser
"The Src kinase, Yes, is activated by CCK and mediates EGFR Shc-Grb2 interactions ( xref )."
sparser
"The concentrations of EGF for which PI 3-kinase inhibitors block Ras activation induce formation of Shc-Grb2 complexes but not detectable EGF receptor phosphorylation and do not activate PI 3-kinase."
sparser
"Surprisingly, EGF and TGF-alpha induced full activation of MAPK in duration and intensity and stimulated association of the EGFR with Shc and Grb2."
sparser
"By contrast, Shc and Grb2 were inducibly associated with the EGFR in response to EGF stimulation of glioma cells."
SOS binds EGFR and GRB2. 10 / 17
| 17
sparser
"Upon EGFstimulation of the cell, binding of Sos to the EGF receptor-Grb2 complex brings a significant portion of Sos to the membrane (Figure 5)."
sparser
"EGF treatment of cells promotes the formation of a complex of EGF receptor, Grb2, and Sos."
sparser
"The measurable catalytic exchange activity of Sos in immunocomplex is unaltered by the association of Sos with EGF receptor and Grb2, either when the association occurs in EGF- stimulated cells or by [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"In contrast to the activated epidermal growth factor receptor that binds the GRB2-Sos complex directly, activation of the insulin receptor results in the interaction of GRB2-Sos with IRS-1 and Shc, thus linking the insulin receptor to Ras signaling pathways."
sparser
"As expected, mutation of the Grb2 binding site significantly reduced the interaction of Grb2 and SOS with EGFR ( xref )."
sparser
"We only considered this effect for the assembly of the EGFR-Grb2-Sos complex, but other components of the EGRF signaling network may also decrease their mobility upon activation."
sparser
"Zhang used this system to demonstrate the Grb2-mediated interaction of the cytoplasmic domain of the epidermal growth factor receptor with the Sos protein."
sparser
"Following EGFR activation, Grb2 and Sos (adaptor proteins) bind EGFR directly at Y1092 and Y1110 or through Shc (which binds at EGFR Y1172 and Y1197) [ xref ]."
sparser
"If the stimulation of exchange activity measured in this permeabilized cell system was due to the formation of EGF receptor-Grb2-Sos complexes, then it may be possible to inhibit it by the use of t[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"In response to epidermal growth factor (EGF), the activated EGF receptor binds to a complex containing the signaling protein GRB2 and the Ras guanine nucleotide-releasing factor Sos, leading to activation of the Ras signaling pathway."
EGF binds EGFR and GRB2. 10 / 16
2 | 14
sparser
"Upon EGF stimulation, Grb2 binds phosphorylated EGFR and activates the signaling pathway xref , xref ."
sparser
"Together these data indicate that AG-1478 inhibits both constitutive as well as EGF-induced EGFR-Grb2 interaction."
sparser
"Preclinical studies revealed cetuximab could decrease cell proliferation and phosphorylation of EGFR, and blocked the binding of the adaptor protein Grb2 to EGFR upon activation by EGF [ xref ]."
sparser
"Interestingly, in spite of the free phosphonate group with its negative charges, CGP78850 inhibited association of Grb2 with EGFR in intact serum-starved MDA-MB-468 triple-negative breast cancer cells stimulated with EGF in vitro ."
sparser
"Our results support the concept that EGFR interacts with Grb2 in both constitutive and EGF-dependent manners and this interaction is independent of HER3 co-expression."
sparser
"1) Association of Grb2 to EGFR in BxPC-3 induced by EGF in the presence of Erbitux indicates an alternate pathway of Ras-MAPK activation, which may be related with the tumor resistance to treatment; 2) transactivation of EGFR downstream Ras-MAPK pathway by FGF contributes the resistance to treatment; and 3) downregulation of EGFR may increase the response to therapy."
sparser
"Compound inhibited epidermal growth factor (EGF)-induced protein–protein interactions between EGFR (EGF receptor) and Grb2 in MDA-MB-468 cells and inhibited Ras activation in the same cell line."
sparser
"We identified that As 2 O 3 activates EGFR and promotes phosphorylation of p66 ShcA and its interaction with the Grb2 adaptor protein with slower kinetics compared to EGF-mediated EGFR activation."
sparser
"Because Ras activation by EGF involves EGF-stimulated association of EGF-R with Grb2, the EGF-R was immunoprecipitated and a Western blot was probed for Grb2."
sparser
"Pre-clinical studies showed cetuximab decreased cell proliferation and phosphorylation of EGFR, and blocked the binding of the adaptor protein Grb2 to EGFR upon activation by EGF [ xref ]."
LRRK1 binds EGFR and GRB2. 7 / 7
| 6 1
sparser
"We recently showed that LRRK1 forms a complex with activated EGFR through a mutual interaction with Grb2 ( xref )."
sparser
"Our results demonstrate that LRRK1 forms a complex with EGFR via Grb2 and associates with STAM and Hrs in response to ligand stimulation."
sparser
"LRRK1 forms a complex with activated EGFR via Grb2."
reach
"Interestingly, LRRK1 has been proposed to regulate endocytosis of EGFR via formation of a LRRK1, Grb2, and EGFR complex, while LRRK2 did not appear to form such a complex in this study suggesting that the two have functionally diverged."
sparser
"Interestingly, LRRK1 has been proposed to regulate endocytosis of EGFR via formation of a LRRK1-Grb2-EGFR complex ( xref ), while LRRK2 did not appear to form such a complex in this study suggesting that the two have functionally diverged."
sparser
"In this paper, we demonstrate that leucine-rich repeat kinase LRRK1, which contains a MAPKKK-like kinase domain, forms a complex with activated EGFR through an interaction with Grb2."
sparser
"We previously reported that LRRK1 forms a complex with activated EGFR through an interaction with Grb2 ( xref )."
SHC binds SOS, EGFR, and GRB2. 4 / 4
| 4
sparser
"HO also induces the association of the Shc-Grb2-Sos complex with the EGF receptor, resulting in activation of downstream signalling systems ."
sparser
"Once the SHC-Grb2-SOS complex is associated with the EGFR, the SOS promotes the exchange of GDP for GTP on Ras, a small G protein."
sparser
"After EGFR activation, a trimeric complex between tyrosine phosphorylated Shc, Grb2, and Sos is formed and this, in turn, triggers downstream mitogenic signaling xref ."
sparser
"The Grb2-SOS complexes that have bound to EGFR or the EGFR-Shc complex catalyse the conversion of RasGDP to RasGTP (reaction 28), whereas the reverse transition is catalysed by RasGAP bound to the RP 2 form of EGFR (reaction 29)."
Tyrosine-phosphorylated EGFR binds GRB2. 3 / 3
| 2 1
trips
"These analyses reveal a hierarchy of interactions between Grb2 and Shc with the EGFR and indicate that Grb2 can bind the tyrosine-phosphorylated EGFR directly, as well as indirectly via Shc."
trips
"Immunoblotting experiments indicate that GRB2 associates with tyrosine-phosphorylated epidermal growth factor receptors (EGFRs) and platelet-derived growth factor receptors (PDGFRs) via its SH2 domain."
reach
"In support of this model, Gab2 mutants defective in 14-3-3 binding exhibit increased recruitment of Grb2 and consequently sustained association with the tyrosine phosphorylated EGFR and Shc."
Phosphorylated EGFR binds GRB2. 3 / 3
| 3
reach
"Upon EGF stimulation, Grb2 binds phosphorylated EGFR and activates the signaling pathway XREF_BIBR, XREF_BIBR."
reach
"One model to emerge is that Grb2, when bound to phosphorylated EGFR, recruits c-Cbl for the purpose of degrading the receptor and terminating RTK signaling in a classic negative-feedback loop."
reach
"Although phosphorylated EGFR can bind to both Shc and Grb2, the predominant linkage was observed between EGFR and Shc."
SOS1 binds EGFR and GRB2. 3 / 3
1 | 2
bel
"In response to stimulation with epidermal growth factor (EGF), the guanine nucleotide exchange factor human SOS1 (hSOS1) promotes the activation of Ras by forming a complex with Grb2 and the human EGF receptor (hEGFR)"
sparser
"Upon EGF stimulation, SOS1 binds to phosphorylated EGFR through the adaptor protein GRB2, which then triggers RAS activation at the plasma membrane ."
sparser
"Although the low-copy-number vector pBT(L) was the best in the case of EGFR-Grb2-Sos1 Y3H interaction, it may also cause reduced bait expression and lowered sensitivity for the detection of weaker protein interactions."
SHC1 binds EGFR and GRB2. 3 / 3
1 | 2
hprd
No evidence text available
sparser
"3.1 As 2 O 3 Induces EGFR and ShcA Phosphorylation and Interaction with Grb2 Adaptor Proteins in HEY Ovarian Carcinoma Cells."
sparser
"It has been reported that after administration of a single receptor-saturating dose of EGF there is a rapid and extensive receptor internalisation with a concomitant tyrosine phosphorylation of 170- a[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
EGFR binds GRB2 and MET. 2 / 2
| 1 1
reach
"On activation of certain RTKs such as EGFR and MET, Gab1 is recruited to the plasma membrane via interaction with EGFR bound Grb2 or directly with MET, followed by the phosphorylation of a number of tyrosine residues on Gab1."
sparser
"Prior co-immunoprecipitation experiments provided indirect evidence that Grb2 is the primary target of 1 and that 1 was able to disrupt Grb2-EGFR and Grb2-c-Met interactions. xref , xref "
ERBB binds EGFR and GRB2. 2 / 2
| 2
sparser
"For example, the SH2 domain of Grb2 and others bind to the phosphorylated Tyr1068 residues of EGFR and ErbB family members at other residues xref ."
sparser
"For example, the SH2 domain of Grb2 and others bind to the phosphorylated Tyr1068 residues of EGFR and ErbB family members at other residues [50] ."
EGFR binds GRB2 and PTK2B. 2 / 2
| 2
sparser
"This translocation is accomplished by activation of protein tyrosine kinases, such as Src family kinases, Bruton’s tyrosine kinase, Pyk2, epidermal growth factor (EGF) or platelet-derived growth facto[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"As Grb2 association with scaffolds, such as EGFR or Pyk2, is vital for their capacity to activate ERK1/2 cascades, we investigated the relative effects of R1- versus R4-level peroxide exposures upon Grb2 interaction with EGFR or Pyk2."
EGFR binds GRB2 and pelA. 2 / 2
| 2
sparser
"In two series of lung adenocarcinoma patients, PLA EGFR:GRB2 was independently associated with longer survival (HR=0.46, CI 95% 0.2–0.78, p=0.0085 and HR=0.48, CI 95% 0.2–0.85, p=0.017)."
sparser
"Low EGFR:GRB2 PLA group (1 st quartile) was independently associated with worse survival in both Yale cohorts A and B. Gender and stage were shown to be independent prognostic markers only in Yale cohort A."
EGFR binds GRB2 and p66shc. 2 / 2
| 2
sparser
"Indeed, during severe oxidative stress, increased binding of p66shc to the activated EGFR and Grb2 occurs."
sparser
"However, during severe oxidative stress, increased binding of p66shc to the activated EGFR and Grb2 occurs."
EGFR binds GRB2 and yfiA. 2 / 2
| 2
sparser
"However, we observed increased Grb2 binding to pY-EGFR (for both EGF and As 2 O 3 ) ( xref )."
sparser
"Upon ligand binding to ERBB1, also known as EGFR, the E3-ubiquitin ligase Cbl is recruited directly to EGFR phosphotyrosine 1045 (pY1045) or indirectly through Grb2, which binds EGFR pY1068 and pY1086[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
EGFR binds GAB1 and GRB2. 2 / 2
| 2
sparser
"It is well established that Gab1 binds directly and specifically to Met , and strong evidence indicates that Gab1 binds EGFR indirectly via Grb2 , although direct binding has also been suggested ."
sparser
"Gab1 interacts with EGFR via constitutively bound Grb2, becomes phosphorylated and binds PI3-K, resulting in its activation, consequently, enhancement of PIP3 level in the cell membrane."
GST binds EGFR and GRB2. 2 / 2
| 2
sparser
"To determine whether complement-induced EGF-R tyrosine phosphorylation correlated with EGF-R activation, we examined if EGF-R interacted with a GST-Grb2 fusion protein."
sparser
"Support for this conclusion was provided by experiments showing that GST-Grb2 fusion protein bound the complement-transactivated EGF-R (), and that complement-dependent ERK2 and cPLA activation were b[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
EGFR binds GRB2 and PLCG1. 2 / 2
| 2
sparser
"Furthermore, PLC-γ1 Y783F as well as PLC-γ1 WT specifically interacts with EGFR, suggesting that EGFR may not involve in the interaction between PLC-γ1 and Grb2."
sparser
"To elucidate whether the tyrosine phosphorylated PLC-γ1 indirectly interacts with Grb2 through EGFR, we generated tyrosine phosphorylation-defective mutants in which tyrosine residues 771, 783 and 125[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
SHC binds EGFR, GRB2, and IRS1. 2 / 2
| 2
sparser
"Furthermore, we have identified a short sequence motif (YV/IN) present in IRS-1, EGFR and Shc, which specifically binds the SH2 domain of GRB2 with high affinity."
sparser
"24 GRB2 over-expression is particularly interesting in breast cancer because GRB2 can bind to tyrosine-phosphorylated EGFR, SHC, insulin receptor substrate-1, and HER2/neu,1'15'26'27 which have all be[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
SRC binds BLNK, EGFR, and GRB2. 2 / 2
| 2
sparser
"The Src homology 2 (SH2) domain of Grb2 binds to autophosphorylation sites of EGFR, including Y1068, and several other receptors."
sparser
"Phosphorylated EGFR binds the Src homology 2 (SH2) domain of the adaptor protein GRB2, which in turn recruits the guanine nucleotide exchange factor SOS."
EGFR binds GRB2, CFP, and luxY. 2 / 2
| 2
sparser
"In particular, FRET measurements indicated that activated EGFR-CFP interacted with Grb2-YFP in membrane ruffles and endosomes."
sparser
"Previously, Sorkin et al. xref used FRET microscopy to image the association of EGFRCFP with Grb2YFP in porcine aortic cells using filter-based fluorescence imaging."
SHC binds EGF, EGFR, and GRB2. 2 / 2
| 2
sparser
"Dominant-negative forms of SHP-2 had no effect on EGF-stimulated interaction of GRB2 with EGFR or SHC, nor did they influence phosphorylation of SHC and SHC/EGFR association."
sparser
"Experiments with rat liver have demonstrated that following the administration of a bolus of EGF, intracellular EGFR are associated with Shc, Grb2, and mSOS [73] ."
sparser
"Stimulation with ufCB also induced processing of membrane-anchored proheparin-binding (HB)-EGF, release of soluble HB-EGF into the medium, association of phosphorylated EGF-R and Shc with glutathione-S-transferase-Grb2 fusion protein, and phosphorylation of extracellular signal-regulated kinase (ERK)."
sparser
"ET-1 rapidly increased association of EGFR and Shc with glutathione-S-transferase-Grb2 fusion protein."
| 2
sparser
"The role of EGFR interaction with the Grb2 adaptor protein and Cbl ubiquitin ligase, and receptor ubiquitination in the clathrin-dependent internalization and sorting of EGFR in multivesicular endosomes is discussed."
sparser
"The role of EGFR interaction with the Grb2 adaptor protein and Cbl ubiquitin ligase, and receptor ubiquitination in the clathrin-dependent internalization and sorting of EGFR in multivesicular endosomes is discussed."
RAF binds RAS, SOS, EGFR, and GRB2. 2 / 2
| 2
sparser
"The EGFR interacts with adaptor proteins Grb2 and SOS, activating Ras and Raf, followed by phosphorylation of Mitogen-activated protein kinase/ERK and extracellular signal–regulated kinase (ERK)."
sparser
"Overexpression and/or mutant forms of EGFR, GRB-2, SOS, RAS and RAF are reported to cause oncogenic transformation (Bi- shop, 1991; McCormick, 1993; Cuadrado et al., 1993; Egan and Weinberg, 199[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
CISH binds EGFR and GRB2. 1 / 1
| 1
sparser
"In mammalian cells, Grb2 can interact with negative regulators of EGFR signaling such as the suppressor of cytokine signaling (SOCS) and the SH2 domain inositol 5-phosphatases (SHIPs) [23,42] ."
TOM1L1 binds EGFR and GRB2. 1 / 1
| 1
sparser
"Shc may thus mediate interaction of Tom1L1Grb2 complex with EGFR."
Phosphorylated EGFR binds GRB2 and SH2 domains. 1 / 1
| 1
reach
"Cbl proteins are recruited to the activated EGFR either by the direct binding of their TKB domain to the phospho-tyrosine residue at position 1045 in the EGFR, or by an indirect mechanism mediated by Grb2, as the SH3 domains of Grb2 bind the proline rich region of Cbl proteins and the SH2 domains of Grb2 binds the phosphorylated EGFR."
PDGF binds EGFR and GRB2. 1 / 1
| 1
sparser
"Although GRB2 binds to the EGF receptor and the PDGF #-receptor, it does not become tyrosine phosphorylated in response to 48 L. Claesson-Welsh ligand stimulation."
RHOU binds EGFR and GRB2. 1 / 1
| 1
sparser
"Moreover, RhoU physically associates with activated EGFR in a GRB2-dependent manner through specific proline-rich motifs within its N-terminus."
EGFR binds GRB2 and SH. 1 / 1
| 1
sparser
"After its autophosphorylation of EGFR, it binds the adaptor protein Grb2 through Grb2 SH-2 domain, and activates downstream effectors including Ras, Raf-1, MAPK, etc, in turn."
EGFR binds GRB2 and PTPN1. 1 / 1
| 1
sparser
"Protein phosphatase PTP1B binds to EGFR through Grb2 adaptor protein to terminate EGFR signaling and protein phosphatase MKP2 terminates the Erk1/2 signaling."
EGFR binds GRB2 and glaH. 1 / 1
| 1
reach
"The PH domain mediated recruitment of Gab proteins provides the opportunity to modulate their membrane residency by PI3K and lipid phosphatases in a very dynamic manner without the need to disrupt large signalosomes such as the EGFR, Grb2, and Gab complex that are potentially stabilised by various direct and indirect protein protein interactions."
WAS binds EGFR and GRB2. 1 / 1
| 1
sparser
"She H-Y, Rockow S, Tang J, Nishimura R, Skolink EY, Chen M, Margolis B, Li W: Wiskott-Aldrich syndrome protein is associated with the adapter protein Grb2 and the epidermal growth factor recepto[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
Phosphorylated EGFR binds GRB2 and (SH2) domains. 1 / 1
| 1
reach
"In this study, we generated fusion proteins containing one or two Src-homology 2 (SH2) domains of growth factor receptor bound protein 2 (Grb2), which bind to phosphorylated EGFR, added with HIV-1 transactivating transcription for cell membrane penetration (termed TSF and TSSF, respectively)."
| 1
sparser
"Also, severe oxidative stress (unlike ligand stimulation and moderate oxidative stress, both of which support survival) increased binding of p66(shc) to the activated EGFR and Grb2."
EGFR binds GRB2 and Y1110. 1 / 1
| 1
reach
"Following EGFR activation, Grb2 and Sos (adaptor proteins) bind EGFR directly at Y1092 and Y1110 or through Shc (which binds at EGFR Y1172 and Y1197) [XREF_BIBR]."
SRC binds EGFR and GRB2. 1 / 1
| 1
sparser
"Combined cell lysates were affinity-purified over the SH2 domain of the adapter protein Grb2 (GST-SH2 fusion protein) that specifically binds phosphorylated EGFR and Src homologous and collagen (Shc) protein."
RTK binds EGFR and GRB2. 1 / 1
| 1
sparser
"The internalisation step requires association of EGFR with signalling molecules such as Grb-2 and activity of the receptor tyrosine kinase ; and the tyrosine kinase activity is also required in targe[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
SHC2 binds EGFR and GRB2. 1 / 1
| 1
sparser
"Single molecule pulldowns reveal that an EGFR ligand-stimulated interaction between Shc2 and Grb2 reduces Grb2 availability to bind to the EGFR phospho-sites, leaving these sites available for phospha[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
EGFR binds GRB2 and SH2 domain. 1 / 1
| 1
reach
"These discoveries were extended to mammalian systems, where it was shown that the Grb2 SH2 domain could inducibly bind the tyrosine phosphorylated epidermal growth factor receptor (EGFR) while bound constitutively to son of sevenless (SOS), a guanine nucleotide exchange factor for Ras."
EGFR binds GRB2 and p66Shc. 1 / 1
| 1
sparser
"Increased binding of p66Shc to activated EGFR and Grb2 occurs during severe oxidative stress."
Phosphorylated EGFR binds GRB2 and SH2 domain. 1 / 1
| 1
reach
"This begins with the recruitment of Grb2, which contains an SH2 domain and can bind either directly to the phosphorylated EGFR, or to Shc, an adapter protein that becomes associated with and tyrosine phosphorylated by the EGFR."
SYK binds EGFR and GRB2. 1 / 1
| 1
sparser
"Immunoprecipitation suggested co-association of Syk with epidermal growth factor receptor and GRB-2."
HBEGF binds EGFR and GRB2. 1 / 1
| 1
sparser
"The HB-EGF induced association of Grb2 with EGFR that correlated with pEGFR ( Figure 5 A)."
MAPK binds EGFR and GRB2. 1 / 1
| 1
sparser
"Phosphorylation site 1068 of EGFR forms a complex with Grb2 and increases mitogen-activated protein kinase activation [ xref ]."
PLCG binds EGFR and GRB2. 1 / 1
| 1
sparser
"The association of Grb2 and PLCγ with a 5YF mutant of the EGFR cytoplasmic domain has not previously been reported."
RPS6 binds EGFR and GRB2. 1 / 1
| 1
sparser
"Image 5 Fig. S6 [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
EGFR binds GRB2 and GSTP1. 1 / 1
| 1
sparser
"As shown in Figure xref , GSTP1 and GRB2 could be detected in the immunoprecipitation complex of phospho-EGFR antibody in 30 ng/mL EGF-stimulated CNE2 cells, and could not be detected by the pretreatment of the cells with 1 μm EGFR inhibitor PD153035, which indicates that GSTP1 and GRB2 can interact with phospho-EGFR, are downstream targets of EGFR signaling pathway."
EGFR binds ERBB3 and GRB2. 1 / 1
| 1
sparser
"Importance of the Grb2 binding sites in HER3 for Grb2 interaction with EGFR-HER3 heteromers."
EGFR binds GRB2 and PTPN11. 1 / 1
| 1
sparser
"In Tibetans, three main signaling pathways associated with GRB2, EGFR, and PTPN11 were shown on a network to be significantly downregulated when compared with Han Chinese ( xref )."
SHC binds SOS1, EGFR, and GRB2. 1 / 1
| 1
sparser
"In response to calcium influx, Shc, Grb2, and Sos1 inducibly associate with a 180-kDa tyrosine-phosphorylated protein, which was determined to be the epidermal growth factor receptor (EGFR)."
SHC binds EGFR, GRB2, and MAP3K1. 1 / 1
| 1
sparser
"Thus, it appears that activation of JNK by EGF may involve a transient association of the Grb2MEKK1 complex with Shc and the EGF receptor, causing a transient plasma membrane localisation of MEKK1 an[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
SHC binds EGFR, GRB2, and COS7. 1 / 1
| 1
sparser
"However, Grb2 and Shc in the mitotic COS7 cells did not bind to activated EGFR."
EGFR binds GRB2, PI3-kinase, and homolog 2. 1 / 1
| 1
sparser
"Using the BioNet tool, a sub-network with EGFR at the core was screened from the PPI network, in which EGFR was associated with v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 ( ERBB2 ), growth factor receptor-bound protein 2 ( GRB2 ) and phosphoinositide-3-kinase, regulatory subunit 1 ( PIK3R1 ) ( xref )."
SHC binds TAC1, EGFR, and GRB2. 1 / 1
| 1
sparser
"SP induced formation of an activated EGFR complex containing the adapter proteins SHC and Grb2, but not c-Src."
ERK binds SHC, EGFR, and GRB2. 1 / 1
| 1
sparser
"In line with our observation of persistence ERK activity, ligand-independent EGFR activation associated with the signaling adaptor proteins, Grb2 and Shc."
PKA binds EGFR, GRB2, and rI. 1 / 1
| 1
sparser
"A prime example is the demonstration that the RI and C subunits of PKA physically interact with the activated EGFR in a Grb2-dependent manner [ xref ]."
RAS binds SHC, EGFR, and GRB2. 1 / 1
| 1
sparser
"We also show that the truncated EGF receptor constitutively associates with the adapter proteins Shc and Grb2 which are involved in the recruitment of Ras to activated receptors."
SHC1 binds EGFR, GRB2, and PLEC. 1 / 1
1 |
hprd
No evidence text available
SHC1 binds EGFR, GRB2, and Sos. 1 / 1
| 1
sparser
"Stimulation of EDNRB in Müller cells resulted in phosphorylation of tyrosine residue 1173 of the EGFR, which is one of the major autophosphorylation site that allows binding of GRB2, SOS and SHC adapter proteins to EGFR and that mediates Ras-Raf activated MAPK/ERK signaling [ xref ]."
PPP2 binds SHC, EGFR, and GRB2. 1 / 1
| 1
sparser
"Consistent with this observation, H(2)O(2) stimulated EGFR tyrosine phosphorylation and complex formation with Shc-Grb2 that was abolished by PP2, implicating Src in H(2)O(2)-induced EGFR activation."
RAS binds EGF, EGFR, and GRB2. 1 / 1
| 1
sparser
"This effect has been associated with reduced EGF-induced EGF receptor (EGFR) tyrosine phosphorylation, Grb-2 association, and Ras activation."
CBL binds EGF, EGFR, and GRB2. 1 / 1
| 1
sparser
"Consistent with the autophosphorylation data, the EGF-induced interaction of c-Cbl and Grb2 proteins with EGFR was elevated in the controls and lowered in the HER2 cells ( xref ), while the reverse was true for Gab1, p85 (subunit of PI3K) and SHP2."
SHC binds EGFR, ERBB2, and GRB2. 1 / 1
| 1
sparser
"The SH2 domains of Shc and/or GRB2 bind to tyrosine phosphorylated EGFR or erbB2, bringing the Ras guanine nucleotide exchange factor SOS into the complex, and initiating the ras/MAP Kinase pathway ( [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
CBL binds EGFR, GRB2, and JAK1. 1 / 1
| 1
sparser
"Other mechanisms that could account for the effect of JAK1 on EGFR expression may involve the adaptor protein GRB2 and the ubiquitin ligase CBL, both of which interact with JAK1 and EGFR and modulate EGFR expression ( xref ; xref )."
EGFR binds EGFR, GRB2, and PAK1. 1 / 1
| 1
sparser
"This interaction is independent of EGF stimulation, but it is increased after stimulation of the EGF receptor and EGFR-Grb2-PAK1 interaction is required for EGF induced lamellipodia formation."
RTK binds SOS1, EGFR, and GRB2. 1 / 1
| 1
sparser
"In xref , experimental results show that GRB2 recruits SOS1 to the membrane to form GRB2-SOS1 complex upon the activation of EGFR or other RTKs."
SHC binds TOM1L1, EGFR, and GRB2. 1 / 1
| 1
sparser
"These results suggest that EGF triggers a transient Grb2/Shc-mediated association of EGFR with Tyr-phosphorylated Tom1L1 to engage the endocytic machinery for endocytosis of the ligand–receptor complex."
| 1
sparser
"We also noted reduced Grb2-EGFR interaction with p66 ShcA knockdown in the presence of As 2 O 3 implicating p66 ShcA isoform in mediating this interaction."
MAPK binds RAS, SRC, EGFR, and GRB2. 1 / 1
| 1
sparser
"This resulted in increased binding of another adaptor protein Grb2 to the Src-EGFR complex and the subsequent activation of Ras and MAPKs."
SOS binds SRC, EGFR, GRB2, and JAK3. 1 / 1
| 1
sparser
"This pathway involves interactions with Grb2, Sos and the kinases EGF-R, JAK3 and Src."
SHC binds SRC, BLNK, EGFR, and GRB2. 1 / 1
| 1
sparser
"Also, our data show that EGF receptors are able to phosphorylate SHC, activate the exchange of guanine nucleotide on ras and phosphorylate MAPK by a mechanism that does not require receptor autophosphorylation sites and, therefore, the src homology 2 (SH2):phosphotyrosine-dependent interaction of SHC or GRB2 with the EGF receptor."
SHC binds CPD, EGF, EGFR, and GRB2. 1 / 1
| 1
sparser
"Coimmunoprecipitation studies demonstrated that both EGF and Cpd 5 induced tyrosine phosphorylation of EGFR was associated with increased amounts of adapter proteins Shc and Grb2, and the Ras GTP-GDP exchange protein Sos, indicating the formation of functional EGFR complexes."
SHC binds EGF, EGFR, GRB2, and RasGEF. 1 / 1
| 1
sparser
"As shown in xref , EGF binds the EGF receptor (EGFR) to recruit RasGEFs such as SOS via its SHC domain and Grb2 adapter protein xref , xref , xref , xref ."
PI3K binds SHC, SRC, EGFR, and GRB2. 1 / 1
| 1
sparser
"Using the chimera system, Wang et al [ xref ] identified 4 tyrosine moieties on the MUC1 CT that are phosphorylated upon activation with anti-CD8 antibody as Y20, Y35, Y46, and Y60 which constitute consensus sequences for binding of PI3K, Shc, EGFR or Src, and Grb2, respectively [ xref ]."
EGF binds EGFR, GRB2, eGFP, and mRFP. 1 / 1
| 1
reach
"The amplitude of the autocorrelation function is inversely related to the cluster density of an EGF, EGFR, eGFP, Grb2, and mRFP complex (eq 4)."
| 1
sparser
"As further evidence of BCAR1 coupling to the EGFR pathway, BCAR1 associates with GRB2 and SHC to mediate integrin-mediated adhesion [ xref ] and serum responses [ xref ]."
SHC binds EGFR, GRB2, CFP, and luxY. 1 / 1
| 1
sparser
"Stimulation of cells by EGF resulted in the accumulation of large pools of Grb2-CFP and YFP-Shc in endosomes, where these two adaptor proteins formed a complex with EGFR."
SHC binds SRC, EGFR, AGT, and GRB2. 1 / 1
| 1
sparser
"Furthermore, the association of Src with Shc, Grb2 and the EGFR after angiotensin II stimulation [ xref ], together with previously described Src-mediated phosphorylation of tyrosine residues Y845 and Y1101 of the EGFR [ xref , xref ], suggest an additional means of receptor activation utilized by GPCRs that is distinct from EGFR autophosphorylation."
CBL affects EGFR, and GRB2
| 29 2
CBL binds EGFR and GRB2. 10 / 31
| 29 2
sparser
"Binding of Grb2 and c-Cbl by EGFR is essential for infection."
sparser
"Residual ubiquitination was further consistent with the reported findings that Cbl can also interact indirectly with the EGFR through Grb2 ."
sparser
"However, it has no effect on the indirect binding of c-Cbl to EGFR via Grb2."
sparser
"On the other hand, the binding of Cbl to EGFR via Grb2 is important for receptor internalization [ xref ]."
sparser
"Here we use a simple modelling approach together with experiments to show that the establishment of the threshold requires both the multiplicity of binding sites and cooperative binding of Cbl and Grb2 to the EGFR."
sparser
"Further, only indirect binding of Cbl to the EGFR through Grb2 is considered important for recruitment of the EGFR to clathrin-coated pits and thus essential for ligand-induced endocytosis of the EGF[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"In cell culture studies, the indirect binding of EGFR to Cbl through Grb2 is necessary for receptor internalization, whereas endosomal sorting requires direct binding to Cbl xref , xref ."
sparser
"In addition, Cbl can bind indirectly to the EGFR via Grb2 [ xref ]."
sparser
"Additionally, Cbl can also indirectly bind EGFR through the Grb2 adaptor protein at the Y1068 and Y1086 sites of the receptor xref ."
sparser
"Like c-Cbl, Grb2 bound EGFR more efficiently following EGF or BTC stimulation for 15 min compared with the other EGFR ligands ( xref )."
EGFR affects GRB2, and SHC
| 23
SHC binds EGFR and GRB2. 10 / 23
| 23
sparser
"An earlier study has shown that Grb2 can interact with EGFR indirectly through Shc ( xref ; xref ), enabling the SH2 domain of Grb2 to interact with other proteins."
sparser
"Notably, activated EGFR interacts with Shc and Grb2 not only at the plasma membrane but also in the endosomal compartment, where EGF induces augmented recruitment of Shc and Grb2."
sparser
"We first describe our strategy to inhibit Grb2/Sos interaction at the level of its SH3 domains and then report the results obtained in blocking Grb2 SH2 domain interaction with EGF-R or Shc."
sparser
"Sem-5 is homologous to the mammalian protein Grb2, which binds the autophosphorylated EGF receptor and other phosphotyrosine-containing proteins such as Shc through its SH2 domain."
sparser
"The phosphorylation of these receptors, such as EGFR, also induces the tyrosine phosphorylation of the adapter protein SHC and the association of SHC with Grb2."