A database built with INDRA combining content from numerous readers and databases. This page allows you to curate the loaded statements. For more information please see the manual.

IndraLab

Statements

databases
phosphosite cbn pc11 biopax bel_lc signor biogrid tas lincs_drug hprd trrust | geneways tees isi trips rlimsp medscan sparser reach
reading

EGFR affects EGF
18 2 12 6 | 11 1353 863
16 2 12 6 | 11 1053 845
sparser
"We present a model for how high-affinity EGF binding to the EGF receptor may remove the dimerization arm from its intramolecular binding site, thus promoting receptor dimerization and activation."
sparser
"The current data show that GCN4-ICM forms multimers, because the cross-correlation value for this construct,f c = 0.23, is significantly higher than for the dimer control,f c = 0.13, and slightly higher than for EGF-bound EGFR, = 0.19."
sparser
"The EGF binds to high and low affinity sites in EGFR-expressing cells."
reach
"EGF specifically binds to EGFR, promoting formation of either EGFR-EGFR homodimers or EGFR-ErbB-2 heterodimers and allowing the intracellular tyrosine kinase domains to approximate and undergo transautophosphorylation [XREF_BIBR - XREF_BIBR]."
sparser
"EGF binds to extracellular domain of EGFR, whereas gefitinib, a well-known EGFR inhibitor xref , binds to Tyr kinase domain."
reach
"We propose that this dynamic quenching takes place because EGF bound probes interact with tryptophan residues in the extracellular domain of the EGF and EGFR complex."
reach
"A requirement for negative cooperativity is that EGFR dimers bind one EGF."
sparser
"Next in Fig. xref we compared the MC and ODE concentration profiles of EGF bound EGFR monomer in the presence of ligand (160 nM), with a receptor number density of 125 receptors per μm 2 and a low diffusivity of 2 × 10 -15 m 2 s -1 ."
sparser
"Binding of EGF to its receptor, the epidermal growth factor receptor (EGFR), induces dimerization and activation of EGFR ( xref )."
reach
"EGF and EGFR complex translocates to the nucleus."
TGFA binds EGF and EGFR. 10 / 62
| 62
sparser
"Zalutumumab is a fully human IgG1 mAb that targets EGFR domain III and inhibits binding of EGF and TGF-α to EGFR. xref Zalutumumab also prevents conformational changes in EGFR that are necessary for its activation. xref An open-label, randomized, Phase III trial investigated zalutumumab plus best supportive care (BSC) vs BSC alone in 286 patients with R/M HNSCC after failure of platinum-based chemotherapy. xref Zalutumumab prolonged median PFS compared with BSC alone (9.9 vs 8.4 weeks; P =0.0012)."
sparser
"The natural ligands EGF and TGF-α bind to the extracellular domain of EGFR, and activate the receptor and its downstream signal proteins, ultimately causing activation or modulation of various cellular processes [ xref ]."
sparser
"r to demonstrate autocrine stimulation of choriocarcinoma proliferation via a TGF-a-EGFR regulatory loop, we attempted to disrupt EGFR ligand- receptor interactions in JAr cells by culturing them in [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"We also show that the binding of neuregulin-1beta to ErbB4 and ErbB3 and the binding of betacellulin to both ErbB4 and ErbB1 does not decrease at low pH, unlike the binding of epidermal growth factor and transforming growth factor-alpha to ErbB1."
sparser
"10 Lung cancer cells may produce EGFR ligands, such as epidermal growth factor (EGF) and transforming growth factor alpha, which then bind to the EGFR, leading to paracrine and[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"Binding of epidermal growth factor (EGF) or transforming growth factor alpha (TGF α ) to the extracellular domain of EGFR produces a number of downstream effects that affect phenotypic cell behavior including proliferation, invasion, metastasis, and inhibition of apoptosis [ xref ]."
sparser
"The EGF-R binds two structurally related ligands, epidermal growth factor (EGF) and transforming growth factor-a (TGF-ct)."
sparser
"Both EGF and TGFα binding to the EGFR lead to endocytosis of EGFR, but receptor-EGF complexes are predominantly trafficked for degradation in lysosomes, while TGFα-bound receptor is predominantly recycled back to the cell surface. xref Thus, changes in ligand-dependent activation can radically impact growth factor receptor signaling."
sparser
"Korc, M.; Chandrasekar, B.; Yamanaka, Y.; Friess, H.; Btichler, M.; Beger, H. G. Overexpression of the epidermal growth factor receptor in human pancreatic cancer is associated with concomitant increa[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"Consistent with this, phosphorylated ERK levels were also unaffected by incubation of cells with an antibody that blocks binding of EGF and TGFα to wild-type EGF receptor (data not shown)."
EGF binds EGFR and GRB2. 10 / 16
2 | 14
sparser
"Upon EGF stimulation, Grb2 binds phosphorylated EGFR and activates the signaling pathway xref , xref ."
sparser
"Together these data indicate that AG-1478 inhibits both constitutive as well as EGF-induced EGFR-Grb2 interaction."
sparser
"Preclinical studies revealed cetuximab could decrease cell proliferation and phosphorylation of EGFR, and blocked the binding of the adaptor protein Grb2 to EGFR upon activation by EGF [ xref ]."
sparser
"Interestingly, in spite of the free phosphonate group with its negative charges, CGP78850 inhibited association of Grb2 with EGFR in intact serum-starved MDA-MB-468 triple-negative breast cancer cells stimulated with EGF in vitro ."
sparser
"Our results support the concept that EGFR interacts with Grb2 in both constitutive and EGF-dependent manners and this interaction is independent of HER3 co-expression."
sparser
"1) Association of Grb2 to EGFR in BxPC-3 induced by EGF in the presence of Erbitux indicates an alternate pathway of Ras-MAPK activation, which may be related with the tumor resistance to treatment; 2) transactivation of EGFR downstream Ras-MAPK pathway by FGF contributes the resistance to treatment; and 3) downregulation of EGFR may increase the response to therapy."
sparser
"Compound inhibited epidermal growth factor (EGF)-induced protein–protein interactions between EGFR (EGF receptor) and Grb2 in MDA-MB-468 cells and inhibited Ras activation in the same cell line."
sparser
"We identified that As 2 O 3 activates EGFR and promotes phosphorylation of p66 ShcA and its interaction with the Grb2 adaptor protein with slower kinetics compared to EGF-mediated EGFR activation."
sparser
"Because Ras activation by EGF involves EGF-stimulated association of EGF-R with Grb2, the EGF-R was immunoprecipitated and a Western blot was probed for Grb2."
sparser
"Pre-clinical studies showed cetuximab decreased cell proliferation and phosphorylation of EGFR, and blocked the binding of the adaptor protein Grb2 to EGFR upon activation by EGF [ xref ]."
EGF binds EGFR and ERBB2. 10 / 15
| 15
sparser
"Additionally, AFM was also applied to investigate the effect of Trastuzumab, as well as Pertuzumab, on HER2-modulated EGF-EGFR interactions, which demonstrated that EGF bound to EGFR more stably in the cells co-expressing EGFR and HER2, and the binding enhancement in the presence of HER2 was inhibited by either Trastuzumab or Pertuzumab [ xref ]."
sparser
"For example, binding of two monomeric ErbB2 proteins to an EGF-induced homodimer of EGFR may cause homodimerization of the ErbB2 molecules followed by their trans-autophosphorylation and consequent ac[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"In this manuscript we demonstrate through combination of scattering spectroscopy, electron microscopy, and generalized multiple particle Mie theory (GMT) simulations that the density- and morphology-dependent spectral response of Au nanoparticle (NP) immunolabels bound to the epidermal growth factor receptors ErbB1 and ErbB2 encodes quantitative information of both the cell surface expression and spatial clustering of the two receptors in different unliganded in vitro cancer cell lines (SKBR3, MCF7, A431)."
sparser
"It is assumed that the mechanism of transactivation involves epidermal growth factor binding to EGFR, followed by transphosphorylation of ErbB2 by the active EGFR kinase."
sparser
"Living-cell single-molecule force spectroscopy (SMFS) combined by Atomic Force Microscopy (AFM) was used by this team of scientists to investigate the effect of two monoclonal antibodies used in cancer therapy, Trastuzumab and Pertuzumab, on HER2-modulated EGF-EGFR interaction, demonstrating the utility of this technique in characterizing the effects of protein-based therapeutics on membrane receptors."
sparser
"Thus, it is possible that two ErbB2 proteins could bind to an EGF-induced homodimer of EGFR resulting in their homodimerization, autophosphorylation and subsequent activation ."
sparser
"Results from co-immunoprecipitation experiments and western blot analyses indicate that tyrosine-phosphorylated ErbB2 and EGFR monomers and stable ErbB2/EGFR high molecular complexes (heterodimers) are formed following EGF stimulation, even if the receptors co-immunoprecipitates also in the absence of the ligand; these data suggest the existence of pre-dimerization inactive receptor clusters on the cell surface."
sparser
"EGF predominantly binds EGFR to induce both EGFR homodimers and EGFRHER2 heterodimers, whereas HRG predominantly binds HER3 and HER4, inducing HER2–HER3 and HER2–HER4 heterodimers."
sparser
"Col IV, LM and FN (but not Col I) adhesion reduce EGF binding to its receptors, EGFR and ErbB2 ( xref )."
sparser
"Exposure of 2 or 20 ug/mL 2C4, a HER2 monoclonal antibody which inhibits HER2-HER3 heterodimerization [ xref ], decreased EGF-dependent HER1-HER2 heterodimer formation with a small concomitant increase in HER1-HER1 homodimerization (Figure xref )."
TGFA binds EGF, EGFR, and AREG. 10 / 12
| 12
sparser
"These include: EGF, TGFα and amphiregulin (AREG), which bind specifically to EGFR; heparin-binding EGF-like growth factor, betacellulin and epiregulin which bind to both EGFR and ErbB4 [ xref ]; and the neuregulins/heregulins (HRGs), which are specific for ErbB3 and ErbB4 [ xref ]."
sparser
"From those ligands, EGF, transforming growth factor alpha and amphiregulin bind specifically to EGFR [ xref ]."
sparser
"EGF, AR and TGFα bind EGFR; HRG, BTC and HB-EGF bind EGFR and erbB-4 ( xref )."
sparser
"EGF, transforming growth factor-alpha and amphiregulin bind exclusively to erbB1[ xref ]."
sparser
"However, only EGF, AREG and TGF-α bind EGFR exclusively; other ligands also bind other ERBB family receptors [ xref , xref , xref ]."
sparser
"The EGF-like ligands include EGF, amphiregulin, and transforming growth factor alpha (TGFα), which only bind to the EGF receptor (EGFR), and heparin binding EGF (HB-EGF), betacellulin, and epiregulin, which bind to both EGFR and ErbB4."
sparser
"TGF-α, amphiregulin, and EGF specifically bind EGFR."
sparser
"EGF, TGF-α and amphiregulin bind specifically to EGFR."
sparser
"Whereas EGF, TGF-α and amphiregulin bind to ErbB1, the heregulins (HRGs; also known as neuregulins) bind ErbB3 and ErbB4 [ xref , xref ]."
sparser
"Epidermal growth factor receptor binds primarily to EGF, transforming growth factor-a, and amphiregulin growth factors, while HER3 and HER4 bind HRG and its isoforms."
EGF binds EGFR and transforming growth factor. 10 / 10
| 10
sparser
"When the EGFR extracellular domain binds to its ligands, such as epidermal growth factor (EGF) and transforming growth factor-α (TGF-α), it forms dimers with other EGFR or other HER family members and undergoes autophosphorylation at the key tyrosine residues, thus activating several downstream signalling pathways such as protein kinase B (AKT/PKB) and mitogen-activated protein kinases (MAPK), which regulate multiple cellular processes, including proliferation, survival and apoptosis."
sparser
"Epidermal growth factor (EGF) and transforming growth factor-α bind directly only to EGFR, whereas neuregulins (also known as heregulins) are specific for ErbB3 and ErbB4 xref , xref ."
sparser
"At least two ligands bind to the EGFR, EGF, and transforming growth factor—α."
sparser
"Extracellular ligands, such as epidermal growth factor (EGF) and transforming growth factor-α (TGF-α), can interact with the EGFR [ xref ], resulting in the stimulation of Akt/PI3K and downstream molecules, including mTOR, eNOS, and the Bcl2-associated antagonist of cell death (BAD)."
sparser
"Nimotuzumab, a recombinant humanized monoclonal immunoglobulin G 1 antibody against human EGFR (HER-1), blocks the binding of epidermal growth factor (EGF) and transforming growth factor-α to EGFR."
sparser
"Ligands such as EGF or transforming growth factor bind to EGFR, which stimulates the tyrosine kinase activity of the receptor."
sparser
"Specific ligands, such as epidermal growth factor (EGF) and transforming growth factor-α (TGF-α), bind to EGFR resulting in receptor dimerization and activation of tyrosine kinase, with receptor auto-phosphorylation and downstream signal transduction. xref – xref Signaling through EGFR has been reported to induce proliferation, invasion, and angiogenesis of tumor cells. xref , xref EGFR is frequently overexpressed in both cell lines and tissues of NSCLC xref – xref and overexpression has been reported to be associated with higher incidence of lymph node metastasis, advanced stage, poor differentiation, and a worse prognosis. xref – xref However, several reports, including a meta-analysis failed to confirm a correlation between overexpression of EGFR and patient outcome. xref , xref In addition, no correlation was found between EGFR expression and tumor response to gefitinib, an EGFR tyrosine kinase inhibitor, in patients with NSCLC. xref Therefore, the clinical significance of EGFR expression in NSCLC is still controversial."
sparser
"The data presented by Doctor Schwab indicated that the anti-epidermal growth factor receptor antibody was no better than C225 for blocking epidermal growth factor/transforming growth factor-α binding [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"When a ligand, such as epidermal growth factor or transforming growth factor-α, binds to EGFR, EGFR dimerizes and a downstream signaling pathway is initiated via the phosphoinositide 3-kinase (PI3K)/Akt and mitogen-activated protein kinase (MAPK) pathways."
sparser
"It has been shown that the overexpression of EGFR is common in ESCC, with an incidence of 50–70%, xref , xref , xref and the overexpression is significantly related to prognosis. xref , xref , xref Nimotuzumab is a recombinant humanized monoclonal IgG1 antibody against human EGFR and blocks the binding of EGF and transforming growth factor‐a to EGFR."
CBL binds EGF and EGFR. 8 / 8
| 8
sparser
"EGF was unable to induce ubiquitination of the serine-mutated EGFR, yet EGF-induced phosphorylation of the c-Cbl-binding site at tyrosine 1045, and c-Cbl-EGFR association, was unaffected."
sparser
"Co-immunoprecipitation studies demonstrated that Cbl became associated with the EGF receptor in an EGF-dependent manner."
sparser
"Further, c-Cbl associates only with EGF-EGFR homodimers, but not with EGF-EGFR/HER2 heterodimers, HER2, HER3, or HER4 ( Levkowitz et al., 1996 ; Muthuswamy et al., 1999 )."
sparser
"A further immuno-coprecipitation assay was conducted to examine the binding of EGFR and c-Cbl and EGF, but not anisomycin, induced the EGFR binding to c-Cbl (D)."
sparser
"Upon EGF binding, EGFR undergoes rapid phosphorylation, internalization and ubiquitination by the E3 ubiquitin ligase Cbl, which targets it for lysosomal degradation [ xref ]."
sparser
"In order to determine the role of c-CBL in differential degradation of EGFR mutants, we assessed EGFR and c-CBL binding, the effect of c-CBL knockdown on steady state levels of EGFR and c-CBL's effect on EGF and erlotinib-induced EGFR degradation (Figure xref )."
sparser
"The EGF-dependent rapid association of Cbl with the autophosphorylated EGF receptor observed in some [46, 49–54, 56, 57] , but not all [55] , studies is apparently mediated by the N-terminal region [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"EGF-induced c-Cbl-EGFR binding was partially inhibited, and receptor ubiquitination was abrogated in cells expressing Y1045F-EGFR."
SRC binds EGF and EGFR. 8 / 8
| 8
sparser
"We previously demonstrated that c-Src directly associates with EGFR upon EGF stimulation and c-Src activation contributes to HNSCC cell invasion ( xref )."
sparser
"This mechanism involves the EGF-dependent association of c-Src with HER1 and phosphorylation of the receptor by c-Src on residues Tyr 845 and Tyr 1101."
sparser
"We have previously reported that c-Src directly associates with EGFR in HNSCC cells upon EGF stimulation ( xref )."
sparser
"EGFR and Src interaction with EGF stimulation were also analyzed by the same method ( xref )."
sparser
"Furthermore, it has been reported that Src directly associates with the epidermal growth factor receptor (EGFR) in OSCC cells upon stimulation with EGF and phosphorylates Crk-associated substrate (Cas), podoplanin acting downstream of Src and Cas to promote cell migration."
sparser
"In experimental systems this synergistic growth requires EGF-dependent association of c-Src with the EGFR and phosphorylation of Tyr-845 of the receptor by c-Src."
sparser
"Significantly, both herbimycin A and PP2 abrogated ouabain-induced but not epidermal growth factor-induced Src binding to the EGFR and the subsequent EGFR tyrosine phosphorylation."
sparser
"We have previously demonstrated that c-Src directly associates with EGFR upon EGF stimulation in HNSCC cells ( xref )."
EGF binds EGFR and AREG. 6 / 6
| 6
sparser
"Inasmuch as we normalized the concentrations of AR and EGF to similar levels of phosphorylation of EGFR, we did not further investigate the stability of AR-EGFR and EGF-EGFR binding in intracellular vesicles."
sparser
"Several ligands, such as EGF and amphiregulin, bind to EGFR, whereas there is no known high-affinity ligand binding to Her-2."
sparser
"For example, Areg and EGF specifically interact with the EGF receptor to promote the growth of normal epithelial cells [ xref ], which may partially explain the prolonged ductal tree extension."
sparser
"Positive regulators of emmprin expression include amphiregulin and EGF-EGFR interactions [ xref ], and negative regulators include Pinin, a nuclear and cell adhesion-related protein [ xref ], and Caveolin-1 which reduces emmprin glycosylation and associated MMP activity [ xref ]."
sparser
"Monoclonal antibodies directed against the EGF receptor show differential bindings of amphiregulin and EGF to the EGF receptor."
sparser
"For example, the concomitant presence of the epidermal growth factor receptor (EGFR) and its ligands EGF and amphiregulin is associated with enhanced tumor aggressiveness and shorter survival in patients. xref K-ras proto-oncogene and mutations of the p53 tumor-suppressor gene and the Smad4 gene are frequently dysregulated in the tumors."
EGF binds EGFR and MUC1. 5 / 5
| 5
sparser
"EGF-stimulated binding of MUC1 and EGFR was attenuated by lactose and not sucrose, indicating that galectin-3 may facilitate this interaction ( Figure 7 A)."
sparser
"In pancreatic cancer, silencing of galectin-3 has shown to enhance the cell surface interaction between MUC1 and EGFR in the absence of EGF stimulation and reduced the rate of endocytosis of MUC1-EGFR complex which leads to the noticeable cell surface localization of MUC1."
sparser
"The results demonstrate that silencing galectin-3 blocks the EGF-induced association of MUC1 and EGFR ( Figure 7 B)."
sparser
"Moreover, downregulation of galectin-3 by ectopic miR-322 attenuated the EGF-induced interaction between MUC1 and EGFR ( Figure 7 D)."
sparser
"The significance of the MUC1-C-galectin-3 interaction is supported by the demonstration that galectin-3 forms a bridge between MUC1 and the epidermal growth factor receptor (EGFR) and that galectin-3 is essential for EGF-mediated interactions between MUC1 and EGFR."
SHC binds EGF and EGFR. 5 / 5
| 5
sparser
"In co-stimulation experiments, carbachol and EGF activated MAPK in a non-additive fashion; moreover, EGF-induced association of Shc with the phosphorylated EGF receptor was inhibited by carbachol."
sparser
"As expected, in the absence of EGF, Shc is not associated with the EGFR."
sparser
"Pretreatment of serum-starved cells with GSE resulted in 70% to almost complete inhibition of EGF-induced EGFR activation and 50% to complete inhibition of Shc activation, which corroborated with a comparable decrease in EGF-induced Shc binding to EGFR."
sparser
"EGF but Not Thrombin Stimulates EGF Receptor Tyrosine Phosphorylation and Association of Shc with Phosphotyrosine-containing Proteins."
sparser
"However, in aged cells Shc was unable to form stable complexes with EGFR after EGF stimulation."
EGF binds mutated EGFR. 4 / 4
| 4
reach
"A mutant EGF receptor devoid of intrinsic protein-tyrosine kinase activity bound EGF and dimerized normally yet failed to undergo ligand induced internalization."
reach
"EGF and transforming growth factor-a (TGF-a) bound to the mutant EGFR with high affinity and enhanced the intrinsic mutant EGFR kinase activity."
reach
"Mutant EGFR with the deletion of the dimerization loop weakly bind to EGF, but fails to be phosphorylated [XREF_BIBR]."
reach
"This can be justified if both the EGF and MOI bind to the bound mutant EGFR independently at separate locations, which explains the presence of excess biotin in the second interaction."
SHB binds EGF and EGFR. 4 / 4
| 4
sparser
"Because Y-142 and CRM197 bind to HB-EGF and cetuximab binds to EGFR, it is difficult to directly compare the amino acids used for binding by each reagent to inhibit the sHB-EGF-EGFR interaction."
sparser
"This data suggested that sHB-EGF interacts with EGFR during its translocation to the nuclear envelope."
sparser
"EGFR is one of the HB-EGF receptors, and the binding of sHB-EGF to EGFR leads to phosphorylation of EGFR and activation of its downstream signaling."
sparser
"Pro-HB-EGF is cleaved at the cell surface to yield the soluble form of HB-EGF (sHB-EGF). xref Subsequently, sHB-EGF directly binds to both EGF receptor (EGFR) and ErbB4, xref which triggers receptor dimerization and activates downstream kinases, including extracellular signal-regulated kinase (ERK), JNK, and phosphatidylinositol 3-kinase (PI3K). xref It has been reported that osteoblast differentiation and bone formation can be regulated by EGFR signaling."
EGF binds EGFR and ERBB4. 4 / 4
| 4
sparser
"This is the first report to identify the solution structure of the EGF-family ligand monomers that interact with both ErbB-1 and ErbB-4."
sparser
"It exerts its effects by binding to cell-surface EGF receptors (EGFR) ErbB1 and ErbB4 [ xref , xref ] and to the HB-EGF-specific receptor Nardilysin (Nrdc) [ xref , xref ]."
sparser
"EREG (epiregulin) belongs to the EGF growth factor family that binds both ERBB1 and ERBB4."
sparser
"The EGF-like ligands include EGF, amphiregulin, and transforming growth factor alpha (TGFα), which only bind to the EGF receptor (EGFR), and heparin binding EGF (HB-EGF), betacellulin, and epiregulin, which bind to both EGFR and ErbB4."
EGF binds EGFR and PTK2. 4 / 4
| 4
sparser
"Here we identified SRC-3Δ4 as a signaling adaptor to mediate the interaction between EGFR and FAK and EGF signal transduction in promoting cell migration ( xref )."
sparser
"These results substantiate that SRC-3Δ4 plays a key role in mediating the EGF-induced interaction between EGFR and FAK, and in EGF signal transduction from EGFR to FAK to promote cell migration."
sparser
"Importantly, knockdown of SRC-3Δ4 in MDA-MB231 cells hindered EGF-induced interaction between EGFR and FAK ( xref ), and greatly decreased EGF-stimulated FAK phosphorylation on Y925 ( xref )."
sparser
"In this study, we identified SRC-3Δ4 as the missing adaptor protein that bridges the interaction between EGFR and FAK upon EGF stimulation."
ERK binds EGF and EGFR. 4 / 4
| 4
sparser
"After EGF stimulation, activated ErbB1 and ERK associated with the cytoskeleton to a greater extent than did total ErbB1 and total ERK."
sparser
"EGF binds the EGF receptor, resulting in ERK activation."
sparser
"ChIP-Seq analysis of constitutive and EGF-induced genome-wide binding of EGFR and ERK kinase components reveals their colocalization with Pol2 complex."
sparser
"Subsequent EGFR binding by EGF and heparin binding (HB)-EGF was associated with enhanced EGFR tyrosine kinase activity, prolonged ERK activation, and a significant increase in cyclooxygenase-2 (COX-2) expression compared with 18Co cells treated with EGF and HB-EGF alone."
EGF binds EGFR and A431. 3 / 3
| 3
reach
"Using our HSM, we imaged 8 colors of QD labeled Epidermal Growth Factor (QD-EGF) bound to EGFR expressed on live A431 cells."
reach
"Using the [15N,2 H16] BSSDP modified [Lys3, Tyr22] mEGF, we now report the first measurement of the rotational dynamics of the EGF and EGF receptor complex in A431 membrane vesicles by ST-EPR."
reach
"We took advantage of the widely studied internalization and degradation of the EGF and EGFR complex in A431 cells expressing high levels of EGFR and showing normal autophagic activity (XREF_SUPPLEMENTARY)."
EGF binds EGFR and EGFR. 3 / 3
| 3
sparser
"It has been reported that, when EGF-EGF receptor complex is formed by EGF binding to the extracellular domain of EGF receptor on target cells, it is rapidly concentrated in clathrin- coated pits."
sparser
"Kraus, M. H.; Issing, W.; Miki, T.; Popescu, N. C.; Aaronson, S. A. Isolation and characterization of ERBB3, a third member of the BINDING AND ACTION OF AN EGF ANALOGUE 625 ERBB:epidermal growth f[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"EGF binds specifically to ErbB1 or EGFR, the prototypical member of the ErbB family of receptors that includes ErbB2 (HER2, neu), ErbB3 (HER3), and ErbB4 (HER4) [19–21]."
EGF binds EGFR and PDCD6IP. 3 / 3
| 3
sparser
"These results further demonstrate that ALIX V mediates the EGF-induced ALIX interaction with activated and ubiquitinated EGFR."
sparser
"Thus direct recognition of ubiquitinated EGFR by the ALIX V domain is likely to be one of the major causes for EGF-induced ALIX interaction with EGFR."
sparser
"Moreover, Alix is known to be indirectly associate with EGFR and suppress its endocytosis ( xref ) and we observed that galectin-3 regulates the association of Alix and EGFR after EGF stimulation, as this association is diminished in the absence of galectin-3 ( xref )."
EGF binds EGFR and TGF. 3 / 3
| 3
sparser
"Amphiregulin is one of the family of ligands, also including EGF and TGF-, that binds to the EGF receptor ."
sparser
"Nimotuzumab is a recombinant humanized IgG1 monoclonal antibody directed against EGFR. xref Nimotuzumab binds to EGFR, thereby blocking the binding of EGF and TGF‐α to EGFR, and eventually inhibiting the EGFR signaling pathway."
sparser
"Binding of EGF and TGF- to EGFR initiates a signal transduction process that results in the stimulation of cellular proliferation ."
EGF binds EGFR and ESR2. 3 / 3
| 3
sparser
"EGF blocked EGFR-ERβ interaction and E 2 did not affect the inhibition of EGFR-ERβ interaction seen with EGF treatment (Figure xref )."
sparser
"Immunoprecipitation of ERβ in WCE from H1944 cells showed a pattern similar to that seen in H1793 cell lines: EGFR interacted with ERβ in the EtOH- and E 2 - treated H1944 cells and EGF blocked EGFR-ERβ interaction (Figure xref )."
sparser
"EGF blocked ERβ-EGFR interaction and E 2 did not rescue this inhibition in H1793 cells (Figure xref )."
SRC binds STAT3, FAS, EGF, and EGFR. 3 / 3
| 3
sparser
"The pro-survival form of Fas is the signaling hub required for EGF-induced formation of protein complexes consisting of Fas, EGFR, Src, Yes-1, and STAT3."
sparser
"Fas death domain phosphorylation, which switches on the prosurvival signal of Fas, is essential for the EGF-induced formation of a complex consisting of Fas, EGFR, Yes-1, Src, and STAT3."
sparser
"The pY291-Fas is essential for the EGF-induced formation of the Fas-mediated nuclear EGFR/STAT3 signaling complex consisting of Fas, EGFR, Yes-1, Src, and STAT3."
Mutated EGF binds EGFR. 2 / 2
| 2
reach
"3.2 EGF mutants bind cell surface EGFR with higher affinity than wild-type EGF."
reach
"3.3 EGF mutants bind EGFR extracellular domain with faster kinetic on-rates than wild-type EGF."
EGF binds EGFR and NEDD4. 2 / 2
| 2
sparser
"Co-immunoprecipitation assay found that NEDD4 is associated with EGFR complex upon EGF stimulation, and IHC staining indicates that NEDD4 is co-expressed with EGFR in lung adenocarcinoma tumor tissues, suggesting that NEDD4 might mediate lung cancer cell migration by interaction with the EGFR signaling complex."
sparser
"In this research article, we demonstrated that NEDD4 interacts with EGFR upon EGF stimulation in lung cancer cells."
EGF binds EGFR and PTPN11. 2 / 2
| 2
sparser
"Exogenous Gab1 expression in transfected 10W2T cells restored the EGF-dependent association of SHP-2 with EGFR, although it only showed a marginal effect on EGF-dependent mitogenic activity."
sparser
"On the other hand, Gab1 was not detected in 10W2T cells, and the EGF-dependent association of SHP-2 with EGFR was also absent."
CRK binds EGF and EGFR. 2 / 2
| 2
sparser
"Whereas the wild-type CrkII bound to EGFR only after EGF stimulation, CrkII-23 bound to EGFR from before stimulation."
sparser
"In A431 cells, epidermoid carcinoma cells which overexpress EGFR, CrkII was tyrosine-phosphorylated and associated with the EGFR in an EGF-dependent manner."
sparser
"Our results not only suggested oridonin as a strong anticancer agent targeting EGF-EGFR interactions in ROS dependent mechanism, but also highlighted AFM-SMFS as a powerful technique for pharmacodynamic studies by detecting ligand-receptor interactions, which was also expected to be developed into a promising tool for the screening and mechanism studies of drugs."
sparser
"More precise mechanism studies based on AFM-SMFS demonstrated that oridonin treatment could decrease the energy barrier width, increase the dissociation off rate constant and decrease the activation energy of EGF-EGFR complexes in ROS dependent way, suggesting oridonin as a strong anticancer agent targeting EGF-EGFR interactions in cancer cells through ROS dependent mechanism."
LRRK1 binds EGF and EGFR. 2 / 2
| 2
sparser
"We also found that endogenous LRRK1 was able to form a complex with EGFR following EGF stimulation ( xref )."
sparser
"Reyniers et al. (2014) confirmed protein–protein interactions between LRRK1 and EGF-R upon stimulation with EGF."
RTK binds EGF and EGFR. 2 / 2
| 2
sparser
"The binding of EGF to the EGF receptor (EGFR), a receptor tyrosine kinase, leads to EGFR dimerization and autophosphorylation, and induces the activation of downstream signaling pathways, including th[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"Unlike other growth factors such as HB-EGF and the family of neuregulins, xref EGF preferentially binds to EGFR as opposed to other receptor tyrosine kinases of the HER family."
EGF binds EGFR and PFKP. 2 / 2
| 2
sparser
"In addition, KAT5 depletion largely reduced EGF-induced interaction between EGFR and PFKP, which was rescued by expression of rKAT5 ( xref )."
sparser
"In addition, KAT5 depletion largely reduced EGF-induced interaction between EGFR and PFKP, which was rescued by expression of rKAT5 ( Figure 1 H)."
SYK binds EGF and EGFR. 2 / 2
| 2
sparser
"Immunoprecipitation and confocal microscopy further revealed the increased molecular interaction between EGFR and Syk after EGF stimulation."
sparser
"Immunoprecipitation and confocal microscopy further revealed the increased molecular interaction between EGFR and Syk after EGF stimulation."
| 2
sparser
"Since it is the pEGFR and not the Au NPs per se that is responsible for the hypothesized endosomal apoptotic signaling, we evaluated the pEGFR levels associated with NP-EGF at t = 5, 20 and 80 min."
sparser
"To provef this hypothesis, direct evidence for the interaction between PS NPs and EGF-EGFR complex could be needed."
TGFB1 binds EGF and EGFR. 2 / 2
| 2
sparser
"These data demonstrate that in addition to the loss of expression of EGF-R, in vitro aging is associated with a loss of TGF-β1-dependent induction of EGF."
sparser
"Although the mechanism underlying hepatic fibrosis is complex, the multi-functional transmembrane glycoprotein EGFR specifically interacts with EGF and TGF-β1, causing its dimerization and regulating cell growth, proliferation, and differentiation (Voon et al., xref )."
EGF binds EGFR and PTPD1. 2 / 2
| 2
sparser
"Dynamic PTPD1/EGFR-containing signalling complexes are formed even before EGF stimulation as demonstrated by fluorescence cross-correlation spectroscopy (FCCS)."
sparser
"Although, steady state distribution of PTPD1 to actin filaments and adhesion plaques has been previously observed in primary fibroblasts xref , our data show that in cancer cells that over expressed PTPD1, EGFR/PTPD1-containing signalling complexes could be formed at membrane ruffles before EGF stimulation ( xref )."
EGF binds EGFR and NUP214. 2 / 2
| 2
sparser
"Furthermore, EGF Receptor competitive test in human epithelial cancer A431 cell line showed that EGF- IL18 fusion protein can specifically bind with EGFR by competing with native EGF protein."
sparser
"Furthermore, EGF receptor competitive test in human epithelial cancer A431 cell line showed that EGF-IL18 fusion protein can specifically bind with EGFR by competing with native EGF protein."
EGF binds EGFR and egfp. 2 / 2
| 2
sparser
"These results provide quantitative evidence for an EGF-dependent interaction between EGFReGFP and Grb2–mRFP."
sparser
"The results indicate that Grb2–mRFP associated with EGF-EGFReGFP complexes that are more than 4-fold brighter than EGF-EGFReGFP complexes not associated with Grb2–mRFP."
EGF binds EGFR and RET. 2 / 2
| 2
sparser
"RET and EGFR interact only in the presence of EGF and predominantly through the long RET isoform."
sparser
"On further investigation, we demonstrated an EGF induced interaction between EGFR and RET that was abrogated by both EGFR and RET inhibitors, but to differing degrees in the three cell lines, potentially stemming from differences in the origins of the cell line models and their individualized neoplastic properties from the mutation profiles in each tumor model."
SHB binds CCND1, EGF, and EGFR. 2 / 2
| 2
sparser
"The chromatin immunoprecipitation assay showed the association of sHB-EGFEGFR complex with promoter region of cyclin D1 in the cell nucleus and this association was prevented by application of EGFR kinase inhibitor AG-1478."
sparser
"The EGFR kinase inhibitor AG1478 prevented EGFR interaction with cyclin D1 promoter upon sHB-EGF treatment ( xref ), but did not inhibit it completely."
STAT3 binds EGF, EGFR, and NOS2. 2 / 2
| 2
sparser
"As summarized in A, we observed significant and specific EGF-induced binding of both nuclear EGFR and STAT3 to the iNOS promoter."
sparser
"Consistently, quantitative real-time PCR confirmed in vivo EGF-activated binding of nuclear EGFR and STAT3 to the iNOS promoter by 3.3 ± 1.8-fold and 3.6 ± 0.5-fold, respectively."
EGF binds EGFR, INS, and INSR. 2 / 2
| 2
sparser
"Negative cooperativity was also observed both in EGFR-EGF and IR-insulin interactions, suggesting that the underlying mechanisms of activation of these receptors by ligand binding may be similar."
sparser
"Scatchard analysis of the binding of EGFR and IR with EGF and insulin, respectively, yields concave-up or curvilinear plots that indicate the presence of two classes of binding sites."
SHC binds EGF, EGFR, and GRB2. 2 / 2
| 2
sparser
"Dominant-negative forms of SHP-2 had no effect on EGF-stimulated interaction of GRB2 with EGFR or SHC, nor did they influence phosphorylation of SHC and SHC/EGFR association."
sparser
"Experiments with rat liver have demonstrated that following the administration of a bolus of EGF, intracellular EGFR are associated with Shc, Grb2, and mSOS [73] ."
EGF binds EGFR, EREG, and AREG. 2 / 2
| 2
sparser
"Several ligands, such as EGF, amphiregulin, or epiregulin, bind specific extracellular domains of the EGFR, which activates an intracellular signalling cascade via different signalling pathways."
sparser
"Our results imply that SPHK1 may be the central controller of amplification loops of EGF, AREG and EREG-EGFR interactions that can contribute to cancer progression."
GOLM1 binds EGF, EGFR, and tmd. 2 / 2
| 2
sparser
"In addition, Co-IP analysis demonstrated a decreased capability of GOLM1 TMD interaction with EGFR over time after EGF stimulation, which further suppressed downstream AKT phosphorylation ( xref and xref )."
sparser
"In addition, co-IP analysis demonstrated a decreased capability of GOLM1 TMD interaction with EGFR over time after EGF stimulation, which further suppressed downstream AKT phosphorylation ( Figures 7[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
Ubiquitinated EGF binds EGFR. 1 / 1
| 1
reach
"This is the first demonstration that ALIX is required for sorting of stress internalized EGFR onto ILVs, but, in agreement with previous studies, ALIX is dispensable for sorting of ubiquitinated EGF bound EGFR onto ILVs XREF_BIBR."
EGF binds EGFR-G428D. 1 / 1
| 1
reach
"EGFR Gly428Asp binds to EGF with reduced affinity invitro."
EGF binds EGFR and de4. 1 / 1
| 1
sparser
"With minimal EGF­binding activity, de4 EGFR underwent ligand-independent autophosphorylation and self- dimerization."
EGF binds EGFR and mCherry. 1 / 1
| 1
sparser
"Biolayer interferometry data on protein-protein interaction of EGF-mCherry with the soluble EGFR are comparable to that of unmodified EGF."
EGF binds EGFR and NH. 1 / 1
| 1
sparser
"Furthermore, Texas red-labeled EGF, which binds to the NH 2 -terminal ligand-binding domain of EGFR, co-localized with the ER lumen protein calregulin ( xref )."
EGF binds EGFR and luc. 1 / 1
| 1
sparser
"However, they probably reflected the fact that the activity of EGFR/Shc-Luc reporter is linear with interaction between EGF and the EGFR-luc containing EGFR dimers while the activity of the Grb2/Shc-luc reporter is the catalytic results of activated endogenous and exogenous EGFR."
CCND1 binds EGF and EGFR. 1 / 1
| 1
sparser
"As shown in xref , disruption of microtubule and dynein by nocodazole and EHNA, respectively, blocked EGF-induced binding of EGFR to the CCND1 promoter, a known target of nuclear EGFR xref by chromatin-IP (ChIP) analysis ( xref )."
EGF binds EGFR and GHR. 1 / 1
| 1
sparser
"While GHR knockout is associated with diminished expression of the EGFR and a concomitant decrease in EGF signaling, GH overexpression results in EGFR overexpression with different effects depending on the signaling pathway analyzed: AKT and ERK1/2 pathways are induced by EGF, while STAT3 and STAT5 activation is heterologously desensitized."
ZPR1 binds EGF and EGFR. 1 / 1
| 1
sparser
"Treatment of mammalian cells with EGF caused decreased binding of ZPR1 to the EGFR and the accumulation of ZPR1 in the nucleus."
CSF3R binds EGF and EGFR. 1 / 1
| 1
sparser
"In addition, whether EGF preferentially binds to either EGFR or G-CSFR following 5-FU is still unknown."
EGF binds EGFR and SEA. 1 / 1
| 1
sparser
"Although there are cross-interactions between EGF family and their receptors from human and mouse based on the structural similarity xref , xref , such as human EGF binding the mouse EGF receptor xref and mouse EGF binding the human EGF receptor xref , we confirmed that the human EGF peptide of the EGF-SEA fusion protein bound the EGFR of S180 cells by showing the interaction of anti-SEA antibody with EGFR-interacting EGF-SEA molecules ( xref ) and performing FACS analysis ( xref )."
CCDC88A binds EGF and EGFR. 1 / 1
| 1
sparser
"Our working model ( xref ) is that after EGF stimulation, GIV initially binds activated EGFR and activates Gαi3 and Akt to regulate EGFR signaling and cell migration at the PM ( xref ), and later GIV binds Gαs at EEA1 endosomes to facilitate EEA1 endosome maturation and down-regulation of proliferative signaling from EEA1 endosomes as shown in the present work."
C7 binds EGF and EGFR. 1 / 1
| 1
sparser
"Disulphide-bridge formation by C3 and C7-EGFRs was, therefore, most dramatically enhanced by EGF, and the disulphide-bonding rate of EGF-bound C7-EGFR is faster than that of the AG1478-bound receptor [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
EGF binds EGFR and PDCD1. 1 / 1
| 1
sparser
"Knockout of B3GNT3 in BT549 cells reduced EGF/EGFR-mediated PD-1 interaction ( xref ) and sensitized cancer cells to T cell killing ( xref )."
EGF binds EGFR and PAEP. 1 / 1
| 1
sparser
"When an excess amount of biotin–PEGEGF conjugate (24 nmol) was added, the extent of GFP gene expression tended to decrease probably due to competitive binding of free biotin–PEGEGF conjugate to EGFR[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
EGF binds EGFR and S180. 1 / 1
| 1
reach
"Although there are cross-interactions between EGF family and their receptors from human and mouse based on the structural similarity XREF_BIBR, XREF_BIBR, such as human EGF binding the mouse EGF receptor XREF_BIBR and mouse EGF binding the human EGF receptor XREF_BIBR, we confirmed that the human EGF peptide of the EGF-SEA fusion protein bound the EGFR of S180 cells by showing the interaction of anti-SEA antibody with EGFR interacting EGF-SEA molecules (XREF_FIG) and performing FACS analysis (XREF_FIG)."
DUSP6 binds EGF and EGFR. 1 / 1
| 1
sparser
"Other studies ( xref ) have noted functional interactions between DUSP6 and EGFR, with EGF treatment inducing DUSP6 mRNA expression, erlotinib treatment depressing DUSP6 mRNA expression in some cell types, and DUSP6 depletion increasing the phosphorylation of ERK1/2 ( xref )."
GRK2 binds EGF and EGFR. 1 / 1
| 1
sparser
"However, overexpression of Gbetagamma scavengers blocked EGF-induced formation of GRK2-EGFR complex."
EGF binds EGFR and AZU1. 1 / 1
| 1
sparser
"MMP can cleave heparin-binding EGF leading to EGFR transactivation ()."
EGF binds EGFR and sef. 1 / 1
| 1
sparser
"Based on these observations, we predicted that Sef might interact with EGFR to affect EGF signaling."
EGF binds EGFR and tyrosine residue. 1 / 1
| 1
reach
"EGF binds to EGFR and causes EGFR specific tyrosine residue phosphorylation, thereby leading to activation of signal transduction pathways, including MAPK and ERK and PI3K and Akt pathways [XREF_BIBR - XREF_BIBR]."
EGF binds EGFR and pdaC. 1 / 1
| 1
sparser
"Studies have shown that EGF and EGFR expression is associated with a loss of PDAC cell differentiation, whereas NGAL expression correlates with a well-differentiated phenotype xref , xref ."
EGF binds EGFR and CSA. 1 / 1
| 1
sparser
"The study demonstrates an association of high levels of EGF and positive EGFR expression in CAG rats."
FERMT2 binds EGF and EGFR. 1 / 1
| 1
sparser
"Interestingly, we found that EGFR binds to Kindlin-2 under conditions with or without EGF ( Supplementary Fig. S3 B, left)."
NINL binds EGF and EGFR. 1 / 1
| 1
sparser
"EGFR-NLPs in the absence of inhibitor were able to bind to EGF, but the addition of 1 μM of unlabeled EGF or the EGFR monoclonal antibody, cetuximab, to the mixture completely inhibited EGFR-NLP binding to EGF ( xref )."
EGF binds EGFR and PTEN. 1 / 1
| 1
sparser
"Using total internal reflection fluorescence microscopy and automated tracking and detection of CCPs, we find EGF bound EGFR and PTEN are enriched in a distinct subset of short-lived CCPs that corresponded with clathrin-dependent EGF-induced signaling."
EGF binds EGFR and MYBL2. 1 / 1
| 1
sparser
"In contrast, we observed EGF-induced in vivo association of nuclear EGFR to the B-Myb promoter and the association is only detected at the G1/S phase and is abolished by EGFR kinase inhibitor."
SFTPD binds EGF and EGFR. 1 / 1
| 1
sparser
"These data suggest that SP-D reduces EGF binding to EGFR through the interaction between the carbohydrate recognition domain of SP-D and N-glycans of EGFR, and downregulates EGF signaling."
EGF binds EGFR and ERBB3. 1 / 1
| 1
sparser
"Similarly, whereas an EGF-induced interaction between EGFR and ERBB3 was markedly enhanced by the presence of ROR1, both kinase activity and a proline-rich domain of ROR1 were shown to be dispensable [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
DUOX1 binds EGF and EGFR. 1 / 1
| 1
sparser
"EGF-induced nuclear EGFR localization in DUOX1-deficient lung cancer cells was associated with altered dynamics of cysteine oxidation of EGFR, and an overall reduction of EGFR cysteines."
GLS2 binds EGF and EGFR. 1 / 1
| 1
sparser
"The aim of this study is to investigate the potential association between single-nucleotide polymorphisms of EGF +61 G/A and EGFR +2073 A/T and NPC."
EGF binds EGFR and IGF1R. 1 / 1
| 1
sparser
"The Di-diabody binds to both EGFR and IGFR and effectively blocked both EGF- and IGF-stimulated receptor activation and tumor cell proliferation."
EGF binds EGFR and Tyr1173. 1 / 1
| 1
reach
"When EGF binds with EGFR, Tyr1173 is one of the autophosphorylation sites that is located on the receptor."
EFEMP1 binds EGF and EGFR. 1 / 1
| 1
sparser
"EFEMP1 interacts with EGFR, blocking EGF from binding and stimulating EGFR signaling."
SHC4 binds EGF and EGFR. 1 / 1
| 1
sparser
"The ShcD PTB domain binds inducibly to EGFR Y1148 after EGF stimulation."
IKBKE binds EGF and EGFR. 1 / 1
| 1
sparser
"In agreement with these findings, our data also show the interaction between wild-type EGFR and IKBKE which is reduced by EGF stimulation ( xref )."
| 1
sparser
"In all cases, the maximum amount of IFN complexes formed is higher in Daudi-EGFR cells ( xref ) than in Daudi cells, with most of the IFN complexes also bound via the EGF subunit to EGFR (blue line)."
EGF binds EGFR and ESR1. 1 / 1
| 1
sparser
"After blocking low-affinity EGFR sites with the mAb 2E9, whose binding did not affect the fluorescence lifetime of Al-EGF, the fluorescence lifetime of donor Al-EGF bound to high-affinity EGFR with ac[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
EGF binds EGFR and ANXA1. 1 / 1
| 1
sparser
"In addition, we found an interaction between EGFR and annexin A1 after 10 min of EGF stimulation that was undetectable after 15 min stimulation ( xref E), consistent with annexin A1 tyrosine phosphorylation occurring prior to MCS formation."
STX6 binds EGF and EGFR. 1 / 1
| 1
sparser
"Reciprocal immunoprecipitation (IP)–western assay further supported this result as indicated by the association of EGFR with syntaxin 6 upon EGF stimulation ( xref )."
EGF binds EGFR and cysteine. 1 / 1
| 1
reach
"Hydrogen peroxide generated during EGF binding to EGFR also oxidizes a catalytic cysteine (Cys797) to sulfenic acid in the ATP binding pocket, which enhances auto-phosphorylation of the receptor XREF_BIBR."
AKT binds EGF and EGFR. 1 / 1
| 1
sparser
"Moreover, MCF-7/CAV1 cells stimulated with EGF display higher EGFR and Akt phosphorylation associated with enhanced proliferative and motility rates, compared to MCF-7 cells."
EGF binds EGFR and KRT19. 1 / 1
| 1
sparser
"These results indicate that the activation of the EGF-EGFR signaling pathway is associated with the development of CK19-positive HCC, and the EGF-induced increase in growth abilities of HCC may account for the poor prognosis of the patients."
EGF binds EGFR and SRC-3Δ4. 1 / 1
| 1
sparser
"As shown in xref , SRC-3Δ4 interacted with EGFR and EGF stimulated SRC-3Δ4's interaction with EGFR, FAK, and PAK1."
SLC35G1 binds EGF and EGFR. 1 / 1
| 1
sparser
"In contrast, post-PNX lung growth is not associated with EGF or EGFR upregulation but with markedly increased SP-A level and moderately increased SP-D level; proSP-B and proSP-C levels did not change."
EGF binds EGFR and drk. 1 / 1
| 1
sparser
"We observed several expected interaction dynamics, including the association of subunits of phosphatidylinositol 3-kinase (PI3K) with InR after insulin stimulus, which likely occurs through the adaptor Chico (IRS) and association of the adaptor Drk (Grb2) with EGFR after EGF stimulus ( xref )."
TOB1 binds EGF and EGFR. 1 / 1
| 1
sparser
"Higher TOB1 mRNA expression was recently shown to correlate with shorter distant metastasis-free survival in breast carcinoma, and TOB1 protein phosphorylation was associated with EGF and EGFR expression and with higher proliferation [ xref ]."
DDX6 binds EGF and EGFR. 1 / 1
| 1
sparser
"Finally, our study strongly suggests a noncompetitive binding of Rck and EGF to EGFR."
SOS1 binds EGF and EGFR. 1 / 1
| 1
sparser
"We find that following EGF stimulation, the Sos1 protein associated with the EGFR is markedly less phosphorylated than the majority of the Sos1 within the cell and there was reduced binding of Grb2 with phosphorylated Sos1 protein in a direct binding assay."
EGF binds EGFR and KLK3. 1 / 1
| 1
sparser
"In addition, the EGF and EGFR genes may be associated with PSA levels in patients with BPH."
SORT1 binds EGF and EGFR. 1 / 1
| 1
sparser
"Interestingly, IP confirmed the EGFR/Δc-sortilin interaction, with an increase in the EGFR/FL-sortilin interaction occurring exclusively after EGF stimulation (Fig.  xref , IB panel), as previously observed in A549 cells."
RHOU binds EGF and EGFR. 1 / 1
| 1
sparser
"Of significance, the RhoU–GRB2 interaction is markedly enhanced following EGF stimulation, which strongly correlates with EGF-triggered RhoUEGFR interactions ( xref )."
EGF binds EGFR and MIF. 1 / 1
| 1
sparser
"CIP treatment, which did not affect the binding of MIF to immunoprecipitated endogenous EGFR and purified His–EGFR extracellular domain ( xref ), had no effect on MIF-inhibited EGF binding to EGFR ( xref ) and EGF-induced EGFR phosphorylation ( xref )."
SPINK1 binds EGF and EGFR. 1 / 1
| 1
sparser
"These data indicate that SPINK1 binds to EGFR at the same position of EGFEGFR binding site, but binding affinity is about half compared to EGFEGFR affinity."
EGF binds EGFR and H1299. 1 / 1
| 1
reach
"This result indicates that the binding of EGFR and EGF in MIIP-HA-overexpressing H1299 cells was not impaired but increased, partially because of the cells ' feedback regulation to maintain growth."
EGF binds EGFR and L1. 1 / 1
| 1
reach
"EGFR binds EGF family members via its L1 (between residues 1-151) and L2 (between residues 312-481) domains [XREF_BIBR]."
USP8 binds EGF and EGFR. 1 / 1
| 1
sparser
"It is not clear why the data of xref showed an opposite effect on EGFR by USP8, but it is supported by their observations that upon EGF stimulation, USP8 directly binds EGFR."
CSF1 binds CXCR4, EGF, and EGFR. 1 / 1
| 1
sparser
"There is direct communication between macrophages and tumor cells importantly mediated by interactions between EGFR-CSF-1 and CXCR4-EGF [ xref ]."
EGF binds EGFR, HGF, and MET. 1 / 1
| 1
sparser
"EGF and HGF bind to their cognate receptor EGFR and c-MET, respectively xref xref ."
EGF binds EGFR, MUC1, and cho. 1 / 1
| 1
sparser
"These observations confirmed a direct interaction between EGFR and MUC1-CT in CHO cells after EGF stimulation."
VEGF binds TGFA, EGF, and EGFR. 1 / 1
| 1
sparser
"The most promising of these agents are cetuximab, which blocks the binding of EGF and transforming growth factor alpha (TGF-alpha) to EGFR, and bevacizumab, which binds free VEGF."
VAV2 binds CBL, EGF, and EGFR. 1 / 1
| 1
sparser
"We therefore hypothesized that Cbl may interact with the EGFR-Vav2 complex in EGF-stimulated cells."
ERK binds EGF, EGFR, and EGFR. 1 / 1
| 1
sparser
"Chromatin immunoprecipitations (ChIP) followed by deep sequencing, ChIP-Seq, revealed that genome-wide binding of epidermal growth factor receptor, EGFR and ERK pathway components at EGF-responsive genes was highly correlated with characteristic mitogen-induced Pol2-profile."
HBEGF binds EGF, EGFR, and AREG. 1 / 1
| 1
sparser
"Upon binding of EGFR ligands such as EGF, HBEGF, or amphiregulin (AREG), EGFR undergoes dimerization and autophosphorylation through its intrinsic tyrosine kinase activity and activates numerous signaling pathways including the protein kinase C (PKC) pathway and the p42/44 mitogen-activated protein kinase (MAPK) pathway [ xref , xref , xref , xref ]."
SRC binds EGF, EGFR, and BCAR1. 1 / 1
| 1
sparser
"As previously mentioned activated beta1 associates with p130CAS, c-Src and EGFR leading to EGFR transactivation at EGF-distinct residues such as Y 1086 and Y 1173 [ xref – xref ]."
SH3KBP1 binds CBL, EGF, and EGFR. 1 / 1
| 1
sparser
"Interestingly, CIN85 is associated with CblEGFR in the endosome following prolonged EGF stimulation, and is delivered together with EGFR for destruction in the lysosome [19 • ] ."
| 1
sparser
"These findings, combined with our recent observation that TIP30 formed a protein complex with ACSL4 and EndoB1 to control EGF-EGFR endocytic trafficking in hepatocytes xref , strongly suggest a novel mechanism by which loss of Tip30 contributes to the development of ER+/PR− tumors, at least in part, through enhancing EGFR signaling in ER+ MECs."
EGF binds EGFR, GAB1, and PTPN11. 1 / 1
| 1
sparser
"Gab1 was detected, and the EGF-dependent formation of the EGFR-Gab1-SHP-2 complex was observed in 10W+8 cells."
EGF binds EGFR, LGI1, and MUC1. 1 / 1
| 1
sparser
"In addition, the increases in EGFR-MUC1-C complexes were associated with partial downregulation of EGFR levels observed in the response to EGF stimulation ( xref ) ( xref ; xref )."
LITAF binds EGF, EGFR, and NOF. 1 / 1
| 1
sparser
"Moreover, TFV ORF080L interacted with human/zebrafish LITAF and impaired EGF-induced EGFR degradation, thereby indicating that TFV ORF080L played a role in endosome-to-lysosome trafficking."
STAT3 binds EGF, EGFR, and PTGS2. 1 / 1
| 1
sparser
"Through ChIP studies, they demonstrated that EGFR and STAT3 could associate with the COX-2 promoter in EGFR wild type cells upon EGF stimulation."
ZFYVE16 binds EGF, EGFR, and MAP. 1 / 1
| 1
sparser
"Here, we attempted to map the signaling events associated with Endofin following activation of EGFR with EGF."
TGFA binds HBEGF, EGF, and EGFR. 1 / 1
| 1
sparser
"Baker's study confirmed that unlike the AREG / EREG genes, the genes that encode other EGFR ligands such as TGFα , HB-EGF , and EGF were not consistently associated with patient outcome, and AREG / EREG failed to co-express with other significant genes."
EGF binds EGFR, ERBB4, and EREG. 1 / 1
| 1
sparser
"Each member has distinct binding affinities to different ligands that allows the classification of ligands into three groups: in the first group, EGF, transforming growth factor- α (TGF- α ) and amphiregulin (AREG) specifically bind to EGFR; in the second group, betacellulin, heparin-binding EGF (HB-EGF) and epiregulin bind to both EGFR and ERBB4; the third class include neuregulins (NRGs) that bind to either only ERBB4 (NRG3 and NRG4) or both ERBB3 and ERBB4 (NRG1 and NRG2)."
CBL binds EGF, EGFR, and GRB2. 1 / 1
| 1
sparser
"Consistent with the autophosphorylation data, the EGF-induced interaction of c-Cbl and Grb2 proteins with EGFR was elevated in the controls and lowered in the HER2 cells ( xref ), while the reverse was true for Gab1, p85 (subunit of PI3K) and SHP2."
SNX6 binds EGF, EGFR, and GIT1. 1 / 1
| 1
sparser
"These data demonstrate an endosomal, EGF-regulated interaction between SNX6 and GIT1 that enhances degradation of the EGFR, and thereby alters EGFR signaling."
TYMS binds EGF, EGFR, and ERBB2. 1 / 1
| 1
sparser
"CHIP demonstrated that EGF-induced nuclear EGFR and HER2 bind to the TS promoter in SNU216 and SKBr3 cells, and that their association was abolished by lapatinib ( xref )."
ERK binds PI3K, EGF, and EGFR. 1 / 1
| 1
sparser
"EGF could increase the movement of HCC cells, especially transforming HCC cells from low to high metastatic capacity, through EGF-EGFR binding, PI3K and ERK signal pathway, or EGFR transactivation of CXCR2."
STAM binds EGF, EGFR, and Flag. 1 / 1
| 1
sparser
"When cells were treated with LRRK1 siRNA, we observed a reduction in the EGF-dependent interaction of activated EGFR with Flag-STAM1 ( xref )."
BTC binds HBEGF, EGF, EGFR, and ERBB4. 1 / 1
| 1
sparser
"The binding of epiregulin to EGFR and ErbB4 makes epiregulin resemble the EGF family members betacellulin and heparin-binding EGF-like growth factor (HB-EGF), both of which bind EGFR and ErbB4 [ xref , xref ], and the EGF family member neuregulin-2beta, which binds EGFR, ErbB3, and ErbB4 ( xref ) [ xref , xref ]."
VEGFR binds DUSP1, EGF, EGFR, and F2R. 1 / 1
| 1
sparser
"In a study on endothelial cells (ECs) Chandrasekharan and colleagues provided an interesting example for a complex metalloproteinase-independent, but EGF-dependent signalling interaction between PAR 1 , the EGFR and the VEGFR, resulting in transcriptional activation by mitogen-activated protein kinase phosphatase 1 (MKP-1), a key signalling mediator in thrombin and VEGF-mediated activation of endothelial cells."
TGFA binds EPGN, EGF, EGFR, and AREG. 1 / 1
| 1
sparser
"Among these, only EGF, TGFα, amphiregulin, and epigen associate specifically with the EGFR homodimer ( xref )."
BTC binds TGFA, EGF, EGFR, and AREG. 1 / 1
| 1
sparser
"Recently, a comparative study xref of EGF, TGFA, AREG, and BTC binding to the EGFR and subsequent dimer formation has made clear that EGF and TGFA have a distinct preference to produce EGFR:ERBB2 heterodimers compared with EGFR:EGFR homodimers, but that BTC and AREG produced dimers of both types equally."
SHC binds CPD, EGF, EGFR, and GRB2. 1 / 1
| 1
sparser
"Coimmunoprecipitation studies demonstrated that both EGF and Cpd 5 induced tyrosine phosphorylation of EGFR was associated with increased amounts of adapter proteins Shc and Grb2, and the Ras GTP-GDP exchange protein Sos, indicating the formation of functional EGFR complexes."
| 1
sparser
"However, similar to endogenous ARAP1, Flag-ARAP1 associated with EGFR and rabaptin 5 after treatment of cells with EGF."
| 1
sparser
"Haglund et al. showed that Spry2 associates with c-Cbl and CIN85 upon EGF stimulation to inhibit EGFR endocytosis and degradation, whereas Spry4 fails to inhibit downregulation of EGFR [ xref ]."
Integrins binds EGF, EGFR, PTK2, and β3. 1 / 1
| 1
sparser
"Both FAK and integrin β3 binding with the EGFR increased in response to EGF."
EGF binds EGFR, GRB2, eGFP, and mRFP. 1 / 1
| 1
reach
"The amplitude of the autocorrelation function is inversely related to the cluster density of an EGF, EGFR, eGFP, Grb2, and mRFP complex (eq 4)."
EGF binds EGFR, HGF, IGF1, and HRG. 1 / 1
| 1
sparser
"To investigate growth factor-induced proliferation we screened a panel of 58 cancer cell lines (10 ovarian cancer, 11 breast cancer, 13 lung cancer, 11 gastric cancer, and 23 colorectal cancer cell lines) for response to the exogenously added ligands EGF, HRG, HGF, and IGF-1 (Supplementary Fig. xref ) that bind to EGFR, ErbB3, Met, and IGF-1R, respectively."
SHB binds EGF, EGFR, ERBB4, and ABL2. 1 / 1
| 1
sparser
"Our results show that Y-142 binds to HB-EGF and ARG and blocks the binding of sHB-EGF to EGFR and ERBB4 and ARG to EGFR."
EGF binds EGFR, ERBB4, EREG, and AZU1. 1 / 1
| 1
sparser
"Betacellulin, heparin-binding EGF and epiregulin bind to both EGFR and HER4."
| 1
sparser
"We found that upon EGF treatment of cells, ARAP1 rapidly and transiently associated with a pre-early endosome that has EGFR, Rab5 and Rabaptin5, but not EEA1."
BTC binds EGF, EGFR, ERBB4, and EREG. 1 / 1
| 1
sparser
"The second group includes β-cellulin (BTC), heparin-binding EGF (HB-EGF) and epiregulin (EPR), which bind to both the EGFR and HER4."
EGF affects EGFR
18 2 12 6 | 11 1353 863
16 2 12 6 | 11 1053 845
sparser
"We present a model for how high-affinity EGF binding to the EGF receptor may remove the dimerization arm from its intramolecular binding site, thus promoting receptor dimerization and activation."
sparser
"The current data show that GCN4-ICM forms multimers, because the cross-correlation value for this construct,f c = 0.23, is significantly higher than for the dimer control,f c = 0.13, and slightly higher than for EGF-bound EGFR, = 0.19."
sparser
"The EGF binds to high and low affinity sites in EGFR-expressing cells."
reach
"EGF specifically binds to EGFR, promoting formation of either EGFR-EGFR homodimers or EGFR-ErbB-2 heterodimers and allowing the intracellular tyrosine kinase domains to approximate and undergo transautophosphorylation [XREF_BIBR - XREF_BIBR]."
sparser
"EGF binds to extracellular domain of EGFR, whereas gefitinib, a well-known EGFR inhibitor xref , binds to Tyr kinase domain."
reach
"We propose that this dynamic quenching takes place because EGF bound probes interact with tryptophan residues in the extracellular domain of the EGF and EGFR complex."
reach
"A requirement for negative cooperativity is that EGFR dimers bind one EGF."
sparser
"Next in Fig. xref we compared the MC and ODE concentration profiles of EGF bound EGFR monomer in the presence of ligand (160 nM), with a receptor number density of 125 receptors per μm 2 and a low diffusivity of 2 × 10 -15 m 2 s -1 ."
sparser
"Binding of EGF to its receptor, the epidermal growth factor receptor (EGFR), induces dimerization and activation of EGFR ( xref )."
reach
"EGF and EGFR complex translocates to the nucleus."
TGFA binds EGF and EGFR. 10 / 62
| 62
sparser
"Zalutumumab is a fully human IgG1 mAb that targets EGFR domain III and inhibits binding of EGF and TGF-α to EGFR. xref Zalutumumab also prevents conformational changes in EGFR that are necessary for its activation. xref An open-label, randomized, Phase III trial investigated zalutumumab plus best supportive care (BSC) vs BSC alone in 286 patients with R/M HNSCC after failure of platinum-based chemotherapy. xref Zalutumumab prolonged median PFS compared with BSC alone (9.9 vs 8.4 weeks; P =0.0012)."
sparser
"The natural ligands EGF and TGF-α bind to the extracellular domain of EGFR, and activate the receptor and its downstream signal proteins, ultimately causing activation or modulation of various cellular processes [ xref ]."
sparser
"r to demonstrate autocrine stimulation of choriocarcinoma proliferation via a TGF-a-EGFR regulatory loop, we attempted to disrupt EGFR ligand- receptor interactions in JAr cells by culturing them in [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"We also show that the binding of neuregulin-1beta to ErbB4 and ErbB3 and the binding of betacellulin to both ErbB4 and ErbB1 does not decrease at low pH, unlike the binding of epidermal growth factor and transforming growth factor-alpha to ErbB1."
sparser
"10 Lung cancer cells may produce EGFR ligands, such as epidermal growth factor (EGF) and transforming growth factor alpha, which then bind to the EGFR, leading to paracrine and[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"Binding of epidermal growth factor (EGF) or transforming growth factor alpha (TGF α ) to the extracellular domain of EGFR produces a number of downstream effects that affect phenotypic cell behavior including proliferation, invasion, metastasis, and inhibition of apoptosis [ xref ]."
sparser
"The EGF-R binds two structurally related ligands, epidermal growth factor (EGF) and transforming growth factor-a (TGF-ct)."
sparser
"Both EGF and TGFα binding to the EGFR lead to endocytosis of EGFR, but receptor-EGF complexes are predominantly trafficked for degradation in lysosomes, while TGFα-bound receptor is predominantly recycled back to the cell surface. xref Thus, changes in ligand-dependent activation can radically impact growth factor receptor signaling."
sparser
"Korc, M.; Chandrasekar, B.; Yamanaka, Y.; Friess, H.; Btichler, M.; Beger, H. G. Overexpression of the epidermal growth factor receptor in human pancreatic cancer is associated with concomitant increa[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"Consistent with this, phosphorylated ERK levels were also unaffected by incubation of cells with an antibody that blocks binding of EGF and TGFα to wild-type EGF receptor (data not shown)."
EGF binds EGFR and GRB2. 10 / 16
2 | 14
sparser
"Upon EGF stimulation, Grb2 binds phosphorylated EGFR and activates the signaling pathway xref , xref ."
sparser
"Together these data indicate that AG-1478 inhibits both constitutive as well as EGF-induced EGFR-Grb2 interaction."
sparser
"Preclinical studies revealed cetuximab could decrease cell proliferation and phosphorylation of EGFR, and blocked the binding of the adaptor protein Grb2 to EGFR upon activation by EGF [ xref ]."
sparser
"Interestingly, in spite of the free phosphonate group with its negative charges, CGP78850 inhibited association of Grb2 with EGFR in intact serum-starved MDA-MB-468 triple-negative breast cancer cells stimulated with EGF in vitro ."
sparser
"Our results support the concept that EGFR interacts with Grb2 in both constitutive and EGF-dependent manners and this interaction is independent of HER3 co-expression."
sparser
"1) Association of Grb2 to EGFR in BxPC-3 induced by EGF in the presence of Erbitux indicates an alternate pathway of Ras-MAPK activation, which may be related with the tumor resistance to treatment; 2) transactivation of EGFR downstream Ras-MAPK pathway by FGF contributes the resistance to treatment; and 3) downregulation of EGFR may increase the response to therapy."
sparser
"Compound inhibited epidermal growth factor (EGF)-induced protein–protein interactions between EGFR (EGF receptor) and Grb2 in MDA-MB-468 cells and inhibited Ras activation in the same cell line."
sparser
"We identified that As 2 O 3 activates EGFR and promotes phosphorylation of p66 ShcA and its interaction with the Grb2 adaptor protein with slower kinetics compared to EGF-mediated EGFR activation."
sparser
"Because Ras activation by EGF involves EGF-stimulated association of EGF-R with Grb2, the EGF-R was immunoprecipitated and a Western blot was probed for Grb2."
sparser
"Pre-clinical studies showed cetuximab decreased cell proliferation and phosphorylation of EGFR, and blocked the binding of the adaptor protein Grb2 to EGFR upon activation by EGF [ xref ]."
EGF binds EGFR and ERBB2. 10 / 15
| 15
sparser
"Additionally, AFM was also applied to investigate the effect of Trastuzumab, as well as Pertuzumab, on HER2-modulated EGF-EGFR interactions, which demonstrated that EGF bound to EGFR more stably in the cells co-expressing EGFR and HER2, and the binding enhancement in the presence of HER2 was inhibited by either Trastuzumab or Pertuzumab [ xref ]."
sparser
"For example, binding of two monomeric ErbB2 proteins to an EGF-induced homodimer of EGFR may cause homodimerization of the ErbB2 molecules followed by their trans-autophosphorylation and consequent ac[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"In this manuscript we demonstrate through combination of scattering spectroscopy, electron microscopy, and generalized multiple particle Mie theory (GMT) simulations that the density- and morphology-dependent spectral response of Au nanoparticle (NP) immunolabels bound to the epidermal growth factor receptors ErbB1 and ErbB2 encodes quantitative information of both the cell surface expression and spatial clustering of the two receptors in different unliganded in vitro cancer cell lines (SKBR3, MCF7, A431)."
sparser
"It is assumed that the mechanism of transactivation involves epidermal growth factor binding to EGFR, followed by transphosphorylation of ErbB2 by the active EGFR kinase."
sparser
"Living-cell single-molecule force spectroscopy (SMFS) combined by Atomic Force Microscopy (AFM) was used by this team of scientists to investigate the effect of two monoclonal antibodies used in cancer therapy, Trastuzumab and Pertuzumab, on HER2-modulated EGF-EGFR interaction, demonstrating the utility of this technique in characterizing the effects of protein-based therapeutics on membrane receptors."
sparser
"Thus, it is possible that two ErbB2 proteins could bind to an EGF-induced homodimer of EGFR resulting in their homodimerization, autophosphorylation and subsequent activation ."
sparser
"Results from co-immunoprecipitation experiments and western blot analyses indicate that tyrosine-phosphorylated ErbB2 and EGFR monomers and stable ErbB2/EGFR high molecular complexes (heterodimers) are formed following EGF stimulation, even if the receptors co-immunoprecipitates also in the absence of the ligand; these data suggest the existence of pre-dimerization inactive receptor clusters on the cell surface."
sparser
"EGF predominantly binds EGFR to induce both EGFR homodimers and EGFRHER2 heterodimers, whereas HRG predominantly binds HER3 and HER4, inducing HER2–HER3 and HER2–HER4 heterodimers."
sparser
"Col IV, LM and FN (but not Col I) adhesion reduce EGF binding to its receptors, EGFR and ErbB2 ( xref )."
sparser
"Exposure of 2 or 20 ug/mL 2C4, a HER2 monoclonal antibody which inhibits HER2-HER3 heterodimerization [ xref ], decreased EGF-dependent HER1-HER2 heterodimer formation with a small concomitant increase in HER1-HER1 homodimerization (Figure xref )."
TGFA binds EGF, EGFR, and AREG. 10 / 12
| 12
sparser
"These include: EGF, TGFα and amphiregulin (AREG), which bind specifically to EGFR; heparin-binding EGF-like growth factor, betacellulin and epiregulin which bind to both EGFR and ErbB4 [ xref ]; and the neuregulins/heregulins (HRGs), which are specific for ErbB3 and ErbB4 [ xref ]."
sparser
"From those ligands, EGF, transforming growth factor alpha and amphiregulin bind specifically to EGFR [ xref ]."
sparser
"EGF, AR and TGFα bind EGFR; HRG, BTC and HB-EGF bind EGFR and erbB-4 ( xref )."
sparser
"EGF, transforming growth factor-alpha and amphiregulin bind exclusively to erbB1[ xref ]."
sparser
"However, only EGF, AREG and TGF-α bind EGFR exclusively; other ligands also bind other ERBB family receptors [ xref , xref , xref ]."
sparser
"The EGF-like ligands include EGF, amphiregulin, and transforming growth factor alpha (TGFα), which only bind to the EGF receptor (EGFR), and heparin binding EGF (HB-EGF), betacellulin, and epiregulin, which bind to both EGFR and ErbB4."
sparser
"TGF-α, amphiregulin, and EGF specifically bind EGFR."
sparser
"EGF, TGF-α and amphiregulin bind specifically to EGFR."
sparser
"Whereas EGF, TGF-α and amphiregulin bind to ErbB1, the heregulins (HRGs; also known as neuregulins) bind ErbB3 and ErbB4 [ xref , xref ]."
sparser
"Epidermal growth factor receptor binds primarily to EGF, transforming growth factor-a, and amphiregulin growth factors, while HER3 and HER4 bind HRG and its isoforms."
EGF binds EGFR and transforming growth factor. 10 / 10
| 10
sparser
"When the EGFR extracellular domain binds to its ligands, such as epidermal growth factor (EGF) and transforming growth factor-α (TGF-α), it forms dimers with other EGFR or other HER family members and undergoes autophosphorylation at the key tyrosine residues, thus activating several downstream signalling pathways such as protein kinase B (AKT/PKB) and mitogen-activated protein kinases (MAPK), which regulate multiple cellular processes, including proliferation, survival and apoptosis."
sparser
"Epidermal growth factor (EGF) and transforming growth factor-α bind directly only to EGFR, whereas neuregulins (also known as heregulins) are specific for ErbB3 and ErbB4 xref , xref ."
sparser
"At least two ligands bind to the EGFR, EGF, and transforming growth factor—α."
sparser
"Extracellular ligands, such as epidermal growth factor (EGF) and transforming growth factor-α (TGF-α), can interact with the EGFR [ xref ], resulting in the stimulation of Akt/PI3K and downstream molecules, including mTOR, eNOS, and the Bcl2-associated antagonist of cell death (BAD)."
sparser
"Nimotuzumab, a recombinant humanized monoclonal immunoglobulin G 1 antibody against human EGFR (HER-1), blocks the binding of epidermal growth factor (EGF) and transforming growth factor-α to EGFR."
sparser
"Ligands such as EGF or transforming growth factor bind to EGFR, which stimulates the tyrosine kinase activity of the receptor."
sparser
"Specific ligands, such as epidermal growth factor (EGF) and transforming growth factor-α (TGF-α), bind to EGFR resulting in receptor dimerization and activation of tyrosine kinase, with receptor auto-phosphorylation and downstream signal transduction. xref – xref Signaling through EGFR has been reported to induce proliferation, invasion, and angiogenesis of tumor cells. xref , xref EGFR is frequently overexpressed in both cell lines and tissues of NSCLC xref – xref and overexpression has been reported to be associated with higher incidence of lymph node metastasis, advanced stage, poor differentiation, and a worse prognosis. xref – xref However, several reports, including a meta-analysis failed to confirm a correlation between overexpression of EGFR and patient outcome. xref , xref In addition, no correlation was found between EGFR expression and tumor response to gefitinib, an EGFR tyrosine kinase inhibitor, in patients with NSCLC. xref Therefore, the clinical significance of EGFR expression in NSCLC is still controversial."
sparser
"The data presented by Doctor Schwab indicated that the anti-epidermal growth factor receptor antibody was no better than C225 for blocking epidermal growth factor/transforming growth factor-α binding [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"When a ligand, such as epidermal growth factor or transforming growth factor-α, binds to EGFR, EGFR dimerizes and a downstream signaling pathway is initiated via the phosphoinositide 3-kinase (PI3K)/Akt and mitogen-activated protein kinase (MAPK) pathways."
sparser
"It has been shown that the overexpression of EGFR is common in ESCC, with an incidence of 50–70%, xref , xref , xref and the overexpression is significantly related to prognosis. xref , xref , xref Nimotuzumab is a recombinant humanized monoclonal IgG1 antibody against human EGFR and blocks the binding of EGF and transforming growth factor‐a to EGFR."
CBL binds EGF and EGFR. 8 / 8
| 8
sparser
"EGF was unable to induce ubiquitination of the serine-mutated EGFR, yet EGF-induced phosphorylation of the c-Cbl-binding site at tyrosine 1045, and c-Cbl-EGFR association, was unaffected."
sparser
"Co-immunoprecipitation studies demonstrated that Cbl became associated with the EGF receptor in an EGF-dependent manner."
sparser
"Further, c-Cbl associates only with EGF-EGFR homodimers, but not with EGF-EGFR/HER2 heterodimers, HER2, HER3, or HER4 ( Levkowitz et al., 1996 ; Muthuswamy et al., 1999 )."
sparser
"A further immuno-coprecipitation assay was conducted to examine the binding of EGFR and c-Cbl and EGF, but not anisomycin, induced the EGFR binding to c-Cbl (D)."
sparser
"Upon EGF binding, EGFR undergoes rapid phosphorylation, internalization and ubiquitination by the E3 ubiquitin ligase Cbl, which targets it for lysosomal degradation [ xref ]."
sparser
"In order to determine the role of c-CBL in differential degradation of EGFR mutants, we assessed EGFR and c-CBL binding, the effect of c-CBL knockdown on steady state levels of EGFR and c-CBL's effect on EGF and erlotinib-induced EGFR degradation (Figure xref )."
sparser
"The EGF-dependent rapid association of Cbl with the autophosphorylated EGF receptor observed in some [46, 49–54, 56, 57] , but not all [55] , studies is apparently mediated by the N-terminal region [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"EGF-induced c-Cbl-EGFR binding was partially inhibited, and receptor ubiquitination was abrogated in cells expressing Y1045F-EGFR."
SRC binds EGF and EGFR. 8 / 8
| 8
sparser
"We previously demonstrated that c-Src directly associates with EGFR upon EGF stimulation and c-Src activation contributes to HNSCC cell invasion ( xref )."
sparser
"This mechanism involves the EGF-dependent association of c-Src with HER1 and phosphorylation of the receptor by c-Src on residues Tyr 845 and Tyr 1101."
sparser
"We have previously reported that c-Src directly associates with EGFR in HNSCC cells upon EGF stimulation ( xref )."
sparser
"EGFR and Src interaction with EGF stimulation were also analyzed by the same method ( xref )."
sparser
"Furthermore, it has been reported that Src directly associates with the epidermal growth factor receptor (EGFR) in OSCC cells upon stimulation with EGF and phosphorylates Crk-associated substrate (Cas), podoplanin acting downstream of Src and Cas to promote cell migration."
sparser
"In experimental systems this synergistic growth requires EGF-dependent association of c-Src with the EGFR and phosphorylation of Tyr-845 of the receptor by c-Src."
sparser
"Significantly, both herbimycin A and PP2 abrogated ouabain-induced but not epidermal growth factor-induced Src binding to the EGFR and the subsequent EGFR tyrosine phosphorylation."
sparser
"We have previously demonstrated that c-Src directly associates with EGFR upon EGF stimulation in HNSCC cells ( xref )."
EGF binds EGFR and AREG. 6 / 6
| 6
sparser
"Inasmuch as we normalized the concentrations of AR and EGF to similar levels of phosphorylation of EGFR, we did not further investigate the stability of AR-EGFR and EGF-EGFR binding in intracellular vesicles."
sparser
"Several ligands, such as EGF and amphiregulin, bind to EGFR, whereas there is no known high-affinity ligand binding to Her-2."
sparser
"For example, Areg and EGF specifically interact with the EGF receptor to promote the growth of normal epithelial cells [ xref ], which may partially explain the prolonged ductal tree extension."
sparser
"Positive regulators of emmprin expression include amphiregulin and EGF-EGFR interactions [ xref ], and negative regulators include Pinin, a nuclear and cell adhesion-related protein [ xref ], and Caveolin-1 which reduces emmprin glycosylation and associated MMP activity [ xref ]."
sparser
"Monoclonal antibodies directed against the EGF receptor show differential bindings of amphiregulin and EGF to the EGF receptor."
sparser
"For example, the concomitant presence of the epidermal growth factor receptor (EGFR) and its ligands EGF and amphiregulin is associated with enhanced tumor aggressiveness and shorter survival in patients. xref K-ras proto-oncogene and mutations of the p53 tumor-suppressor gene and the Smad4 gene are frequently dysregulated in the tumors."
EGF binds EGFR and MUC1. 5 / 5