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phosphosite cbn pc11 biopax bel_lc signor biogrid tas lincs_drug hprd trrust | geneways tees isi trips rlimsp medscan sparser reach
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RAF1 affects MAP2K1
1 16 7 | 2 78 14
1 16 6 | 2 34 13
reach
"PAK1 phosphorylates MEK1 on Ser298, a site important for binding of Raf-1 to MEK1 in vivo."
biogrid
No evidence text available
reach
"An alternative possibility is that adhesion dependent PAK1 activation and subsequent phosphorylation of MEK1 on S298 is sufficient to stimulate MEK1 phosphorylation and activation by low levels of c-Raf activity merely by enhancing the interaction between c-Raf and MEK1."
sparser
"Overexpression of activated forms of c-Raf and MEK1 (a component of the mitogen-activated protein kinase pathway, MAPK) led to cell dissociation, as inferred by the loss of desmosomes from the cell periphery."
sparser
"These results indicated that delphinidin interacts with Raf1 or MEK1, and results in downregulation of their respective kinase activities."
sparser
"Disruption of the interaction between Raf-1 and MEK1, leads to an inhibition of MEK phosphorylation and activation by Raf-1."
sparser
"Of relevance to such possibilities are earlier studies that have shown that Raf1 forms a signaling complex with MEK1, but not MEK2 ( xref ), and that MEK and ERK form a complex together with a scaffold protein that apparently has a preference for MEK1 ( xref ; xref )."
sparser
"We note that NIMA gene-family Neks have been previously implicated in the regulation of various aspects of the cell cycle and that Nek-10 is physically associated with Raf-1 and MEK1, formation of the three-protein complex being necessary for Nek-10-mediated MEK1 autoactivation [ xref , xref ]."
sparser
"Phosphorylation of MEK1 on serine 298 does not appear to regulate the interaction between Raf-1 and MEK1, but rather the ability of Raf-1 to phosphorylate MEK1 with which it is complexed in vivo."
biogrid
No evidence text available
RAS binds MAP2K1 and RAF1. 6 / 6
| 6
sparser
"Within the SGZ of the adult brain, we now report elevations in genes associated with both IL-6Rα and gp130, as well as genes associated with downstream signaling through Ras to activate Raf-1 or MAP2K1."
sparser
"Overexpression of dominant-negative forms of Ras , Raf-1 , and MEK1 in transgenic mice results in defective positive but normal negative selection."
sparser
"Through interaction with Raf-1, RKTG changes the localization of Raf-1 from cytoplasm to the Golgi apparatus, blocks EGF-stimulated Raf-1 membrane translocation, and reduces the interaction of Raf-1 with Ras and MEK1."
sparser
"Transient transfection of Pa-4 cells with active forms of Ras, Raf-1, or MEK1 resulted in decreases of occludin mRNA levels, which can be reversed by the MEK inhibitor PD98059."
sparser
"The binding of PEA-15 was specific to ERK among the components of the ERK MAP kinase pathway, since PEA-15 failed to interact with Ras, Raf1, or MEK1 ."
sparser
"Consistent with the preferential binding of MEK-1 to RAS:RAF-1, the basal activity of MEK-1 in v-ras-transformed cells was found to be elevated sixfold, whereas MEK-2 was elevated only twofold, suggesting that the RAS signalling pathway favors MEK-1 activation."
| 5
sparser
"To identify arrestin-2 elements involved in the binding of c-Raf1, MEK1, and ERK2 we generated 22 mutants in which residues conserved between non-visual arrestins on the non-receptor-binding surface were replaced with alanines individually or in groups ( xref , xref )."
sparser
"Expression of PEBP1 results in production of phosphatidylethanolamine-binding protein 1, a protein that in humans has been shown to interact with C-Raf, MAP2K1 and MAPK1 [ xref ]."
sparser
"MEKK1, an important regulator in stress- and cytokine-induced JNK/SAPK pathways, has also been implicated in the activation of ERK1/2 (Minden et al., 1994; Xu et al., 1996; Yujiri et al., 1998) and [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"The effect of arrestin-2 and arrestin-3 binding to the β2-adrenergic receptor on its interactions with c-Raf1, MEK1, and ERK2 in cellular environment."
sparser
"We examined the importance of this binding site in the feedback phosphorylation of MEK1 on Thr(292) and Thr(386) by ERK2, the phosphorylation and activation of ERK2 by MEK1, and the interaction of MEK1 with ERK2 and Raf-1."
| 3
sparser
"It has been shown that RKIP binds to Raf-1 and MEK1 at overlapping sites [14] ."
sparser
"In the presence of RKIP, RAF1 cannot bind MEK1 ( xref )."
sparser
"A yeast two-hybrid assay screen of a human T cell library was used to identify proteins that bound to Raf-1 kinase binding domains. xref The protein identified was designated RKIP, whose sequence was analogous to the human and monkey 23 kDa protein PEBP. xref Using anti-RKIP antibody, antisense RKIP and sense RKIP expression vectors, Yeung et al. discovered that RKIP could bind Raf-1 and MEK-1, and weakly bind to ERK-2, interfering with MEK phosphorylation and activation by Raf-1."
| 2
sparser
"Wang et al [5] have shown that PEBP4 interacts with Raf-1 and MEK1 and attenuates activation of MEK1."
sparser
"Co-precipitation and in vitro protein binding assay demonstrated that hPEBP4 interacts with Raf-1 and MEK1."
BRAF binds MAP2K1 and RAF1. 2 / 2
| 2
sparser
"When bound to trametinib, the dissociation constants for the interaction of MEK1 with either BRAF or CRAF kinase were 0.101 μM and 0.107 μM, respectively."
sparser
"Moreover, the overexpression of MEK1(3A) failed to block endogenous MEK1 activation, although MEK1(3A) formed complexes with both c-Raf and B-Raf as well as p42/p44 MAPK."
ERK binds MAP2K1 and RAF1. 2 / 2
| 2
sparser
"β-arrestin2 scaffolds appear to act in ERK activation by mediating binding of ERK1/2 and Raf1 and MEK-1."
sparser
"MEK1 but not MEK2 forms a signaling complex with Raf-1, suggesting that in some cells the Raf-1 signaling pathway preferentially activates ERKs via MEK1 ( xref )."
ERK binds MAP2K1, PEBP1, and RAF1. 2 / 2
| 2
sparser
"This mode of action bears striking similarities to the interactions of RKIP with Raf-1 and MEK1 in the MAPK pathway."
sparser
"This mode of action bears striking similarities to the interaction of RKIP with Raf-1 and MEK1 in the MAPK pathway."
RHO binds MAP2K1 and RAF1. 1 / 1
| 1
sparser
"Intriguingly, it is the activation of the core pathway (RAF/MEK/ERK) that generates the phosphorylated forms of RAF1 and MEK1 required for the cross-talk with the RHO and with PI3K pathway, respective[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
| 1
sparser
"Nek10 physically associated with Raf-1 and MEK1 in a Raf-1-dependent manner, and the formation of this complex was necessary for Nek10-mediated MEK1 activation."
| 1
sparser
"The SH2 domain of GRB10 binds to receptor tyrosine kinases and, in a phosphotyrosine-independent manner, GRB10 binds to RAF-1 and MEK1 (Ref. [31] )."
HRAS binds MAP2K1 and RAF1. 1 / 1
| 1
sparser
"In contrast, expression of constitutively active forms of H-Ras, Raf1, or Mek1, in mouse as well as human skin, promotes proliferation and hyperplasia ( xref , xref )."
MAP2K1 binds RAF1 and T292. 1 / 1
| 1
reach
"Frost and co-workers showed that MEK1 with mutations of T292 and S298 to alanine bound c-Raf less efficiently than did wild-type MEK1."
MAP2K1 binds RAF1 and arrestin. 1 / 1
| 1
sparser
"Arrestins that do not bind MEK1 or c-Raf1."
| 1
sparser
"Sustained activation of MAPK3/1 beyond 3 h was independent of EGFR kinase activity but dependent on PKC activity, which appeared to form a self-sustaining MAPK3/1 activation loop by activating RAF1, MAP2K1, and PLA2G4 (phospholipase A2 [all cytosolic isozymes])."
| 1
sparser
"The substrates Raf-1, HSF1, Cdc37, and MEK1 interacted with both Hsp90alpha and Hsp90beta, and the relative patterns of these interactions were not affected by heat shock."
ERK binds MAP2K1, MAPK1, and RAF1. 1 / 1
| 1
sparser
"It also constitutively interacts with Raf-1, MEK1, and ERK2 to facilitate stimulus-induced activation of ERK1/2 ( xref , xref , xref )."
BRAF binds BRAF, MAP2K1, and RAF1. 1 / 1
| 1
sparser
"For example, high expression levels of p-B-Raf p-RAF1 and p-MEK1 were associated with BRAF mutations; high expression levels of p-EGFR, p-SHP-2 and p-Stat3 were linked to EGFR mutations; and high levels of p-Akt or p-HER2 were associated with PIK3CA mutations and ERBB2 mutations, respectively."
| 1
sparser
"Additionally, increased levels of Raf-1 are associated with Ras and MEK1 in Alzheimer's disease as evidenced by its coimmunoprecipitation with Ras and Mek1, respectively."
| 1
sparser
"We found that endogenous hPEBP4 interacts with Raf-1 and MEK1 in TRAIL-treated CaoV-3 cells by co-immunoprecipitation analysis."
| 1
sparser
"It has been shown that Raf-1 and B-Raf interact with both MEK1 and MEK2."
ERK binds MAP2K1, ARRB2, and RAF1. 1 / 1
| 1
sparser
"Therefore, for the analysis of arrestin-2 interactions with c-Raf1, MEK1, and ERK1/2 we only used ten mutants that demonstrated normal receptor binding ( xref ), indicative of proper folding."
MEK binds HRAS, MAP2K1, and RAF1. 1 / 1
| 1
sparser
"Overexpression of transgenes encoding do forms of Raf-1 [68], p21ras [69], Mek-I [70] or both p21ras and Mek-1 [71'] inhibited thymocyre development past the DP stage."
| 1
sparser
"MP-1 selectively interacts with ERK-1 and MEK-1 but not Raf-1, on the surface of cytoplasmic late endosomes and lysosomes [80] ."
| 1
sparser
"Xu et al ( xref ) and Karandikar et al ( xref ) both showed that MEKK1 binds to C-Raf, MEK1 and ERK2 of the ERK1/2 MAPK signaling module."
| 1
sparser
"Downstream of Pak1, both Raf-1 and Mek-1 were associated with Bam32 independently of SLP-76 expression."
AKT binds GRB10, MAP2K1, and RAF1. 1 / 1
| 1
sparser
"For example, Grb10 interacts with Raf1, MEK1 and Akt ( Jahn et al., 2002 ; Nantel et al., 1998 )."
1 |
hprd
No evidence text available
ERK binds KSR1, MAP2K1, RAF1, and flotillin-1. 1 / 1
| 1
sparser
"We here show that flotillin-1 forms a complex with CRAF, MEK1, ERK, and KSR1 (kinase suppressor of RAS) and that flotillin-1 knockdown leads to a direct inactivation of ERK1/2."
ERK binds PHB2, MAP2K1, PHB, and RAF1. 1 / 1
| 1
sparser
"PHB and PHB2 interact with c-Raf to induce the phosphorylation of ERK1/2 via MEK1 [ xref ]."
| 1
sparser
"Moreover, KSR1 is able to translocate from the cytoplasm to the plasma membrane where it forms a complex with Raf-1, MEK1 and 14-3-3 in the presence of activated Ras, supporting its scaffolding function in the MAPKs pathway [ xref , xref ]."
ERK binds JNK, ERBB2, MAP2K1, and RAF1. 1 / 1
| 1
sparser
"HER2 is a well-known upstream kinase of RAF/MAPK, and, therefore, we examined whether BRACs affected the interaction of HER2 with RAF1, MEK1, ERK1/2, and JNK."
MAP2K1 affects RAF1
1 16 7 | 2 78 14
1 16 6 | 2 34 13
reach
"PAK1 phosphorylates MEK1 on Ser298, a site important for binding of Raf-1 to MEK1 in vivo."
biogrid
No evidence text available
reach
"An alternative possibility is that adhesion dependent PAK1 activation and subsequent phosphorylation of MEK1 on S298 is sufficient to stimulate MEK1 phosphorylation and activation by low levels of c-Raf activity merely by enhancing the interaction between c-Raf and MEK1."
sparser
"Overexpression of activated forms of c-Raf and MEK1 (a component of the mitogen-activated protein kinase pathway, MAPK) led to cell dissociation, as inferred by the loss of desmosomes from the cell periphery."
sparser
"These results indicated that delphinidin interacts with Raf1 or MEK1, and results in downregulation of their respective kinase activities."
sparser
"Disruption of the interaction between Raf-1 and MEK1, leads to an inhibition of MEK phosphorylation and activation by Raf-1."
sparser
"Of relevance to such possibilities are earlier studies that have shown that Raf1 forms a signaling complex with MEK1, but not MEK2 ( xref ), and that MEK and ERK form a complex together with a scaffold protein that apparently has a preference for MEK1 ( xref ; xref )."
sparser
"We note that NIMA gene-family Neks have been previously implicated in the regulation of various aspects of the cell cycle and that Nek-10 is physically associated with Raf-1 and MEK1, formation of the three-protein complex being necessary for Nek-10-mediated MEK1 autoactivation [ xref , xref ]."
sparser
"Phosphorylation of MEK1 on serine 298 does not appear to regulate the interaction between Raf-1 and MEK1, but rather the ability of Raf-1 to phosphorylate MEK1 with which it is complexed in vivo."
biogrid
No evidence text available
RAS binds MAP2K1 and RAF1. 6 / 6
| 6
sparser
"Within the SGZ of the adult brain, we now report elevations in genes associated with both IL-6Rα and gp130, as well as genes associated with downstream signaling through Ras to activate Raf-1 or MAP2K1."
sparser
"Overexpression of dominant-negative forms of Ras , Raf-1 , and MEK1 in transgenic mice results in defective positive but normal negative selection."
sparser
"Through interaction with Raf-1, RKTG changes the localization of Raf-1 from cytoplasm to the Golgi apparatus, blocks EGF-stimulated Raf-1 membrane translocation, and reduces the interaction of Raf-1 with Ras and MEK1."
sparser
"Transient transfection of Pa-4 cells with active forms of Ras, Raf-1, or MEK1 resulted in decreases of occludin mRNA levels, which can be reversed by the MEK inhibitor PD98059."
sparser
"The binding of PEA-15 was specific to ERK among the components of the ERK MAP kinase pathway, since PEA-15 failed to interact with Ras, Raf1, or MEK1 ."
sparser
"Consistent with the preferential binding of MEK-1 to RAS:RAF-1, the basal activity of MEK-1 in v-ras-transformed cells was found to be elevated sixfold, whereas MEK-2 was elevated only twofold, suggesting that the RAS signalling pathway favors MEK-1 activation."
| 5
sparser
"To identify arrestin-2 elements involved in the binding of c-Raf1, MEK1, and ERK2 we generated 22 mutants in which residues conserved between non-visual arrestins on the non-receptor-binding surface were replaced with alanines individually or in groups ( xref , xref )."
sparser
"Expression of PEBP1 results in production of phosphatidylethanolamine-binding protein 1, a protein that in humans has been shown to interact with C-Raf, MAP2K1 and MAPK1 [ xref ]."
sparser
"MEKK1, an important regulator in stress- and cytokine-induced JNK/SAPK pathways, has also been implicated in the activation of ERK1/2 (Minden et al., 1994; Xu et al., 1996; Yujiri et al., 1998) and [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"The effect of arrestin-2 and arrestin-3 binding to the β2-adrenergic receptor on its interactions with c-Raf1, MEK1, and ERK2 in cellular environment."
sparser
"We examined the importance of this binding site in the feedback phosphorylation of MEK1 on Thr(292) and Thr(386) by ERK2, the phosphorylation and activation of ERK2 by MEK1, and the interaction of MEK1 with ERK2 and Raf-1."
| 3
sparser
"It has been shown that RKIP binds to Raf-1 and MEK1 at overlapping sites [14] ."
sparser
"In the presence of RKIP, RAF1 cannot bind MEK1 ( xref )."
sparser
"A yeast two-hybrid assay screen of a human T cell library was used to identify proteins that bound to Raf-1 kinase binding domains. xref The protein identified was designated RKIP, whose sequence was analogous to the human and monkey 23 kDa protein PEBP. xref Using anti-RKIP antibody, antisense RKIP and sense RKIP expression vectors, Yeung et al. discovered that RKIP could bind Raf-1 and MEK-1, and weakly bind to ERK-2, interfering with MEK phosphorylation and activation by Raf-1."
| 2
sparser
"Wang et al [5] have shown that PEBP4 interacts with Raf-1 and MEK1 and attenuates activation of MEK1."
sparser
"Co-precipitation and in vitro protein binding assay demonstrated that hPEBP4 interacts with Raf-1 and MEK1."
BRAF binds MAP2K1 and RAF1. 2 / 2
| 2
sparser
"When bound to trametinib, the dissociation constants for the interaction of MEK1 with either BRAF or CRAF kinase were 0.101 μM and 0.107 μM, respectively."
sparser
"Moreover, the overexpression of MEK1(3A) failed to block endogenous MEK1 activation, although MEK1(3A) formed complexes with both c-Raf and B-Raf as well as p42/p44 MAPK."
ERK binds MAP2K1 and RAF1. 2 / 2
| 2
sparser
"β-arrestin2 scaffolds appear to act in ERK activation by mediating binding of ERK1/2 and Raf1 and MEK-1."
sparser
"MEK1 but not MEK2 forms a signaling complex with Raf-1, suggesting that in some cells the Raf-1 signaling pathway preferentially activates ERKs via MEK1 ( xref )."
ERK binds MAP2K1, PEBP1, and RAF1. 2 / 2
| 2
sparser
"This mode of action bears striking similarities to the interactions of RKIP with Raf-1 and MEK1 in the MAPK pathway."
sparser
"This mode of action bears striking similarities to the interaction of RKIP with Raf-1 and MEK1 in the MAPK pathway."
RHO binds MAP2K1 and RAF1. 1 / 1
| 1
sparser
"Intriguingly, it is the activation of the core pathway (RAF/MEK/ERK) that generates the phosphorylated forms of RAF1 and MEK1 required for the cross-talk with the RHO and with PI3K pathway, respective[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
| 1
sparser
"Nek10 physically associated with Raf-1 and MEK1 in a Raf-1-dependent manner, and the formation of this complex was necessary for Nek10-mediated MEK1 activation."
| 1
sparser
"The SH2 domain of GRB10 binds to receptor tyrosine kinases and, in a phosphotyrosine-independent manner, GRB10 binds to RAF-1 and MEK1 (Ref. [31] )."
HRAS binds MAP2K1 and RAF1. 1 / 1
| 1
sparser
"In contrast, expression of constitutively active forms of H-Ras, Raf1, or Mek1, in mouse as well as human skin, promotes proliferation and hyperplasia ( xref , xref )."
MAP2K1 binds RAF1 and T292. 1 / 1
| 1
reach
"Frost and co-workers showed that MEK1 with mutations of T292 and S298 to alanine bound c-Raf less efficiently than did wild-type MEK1."
MAP2K1 binds RAF1 and arrestin. 1 / 1
| 1
sparser
"Arrestins that do not bind MEK1 or c-Raf1."
| 1
sparser
"Sustained activation of MAPK3/1 beyond 3 h was independent of EGFR kinase activity but dependent on PKC activity, which appeared to form a self-sustaining MAPK3/1 activation loop by activating RAF1, MAP2K1, and PLA2G4 (phospholipase A2 [all cytosolic isozymes])."
| 1
sparser
"The substrates Raf-1, HSF1, Cdc37, and MEK1 interacted with both Hsp90alpha and Hsp90beta, and the relative patterns of these interactions were not affected by heat shock."
ERK binds MAP2K1, MAPK1, and RAF1. 1 / 1
| 1
sparser
"It also constitutively interacts with Raf-1, MEK1, and ERK2 to facilitate stimulus-induced activation of ERK1/2 ( xref , xref , xref )."
BRAF binds BRAF, MAP2K1, and RAF1. 1 / 1
| 1
sparser
"For example, high expression levels of p-B-Raf p-RAF1 and p-MEK1 were associated with BRAF mutations; high expression levels of p-EGFR, p-SHP-2 and p-Stat3 were linked to EGFR mutations; and high levels of p-Akt or p-HER2 were associated with PIK3CA mutations and ERBB2 mutations, respectively."
| 1
sparser
"Additionally, increased levels of Raf-1 are associated with Ras and MEK1 in Alzheimer's disease as evidenced by its coimmunoprecipitation with Ras and Mek1, respectively."
| 1
sparser
"We found that endogenous hPEBP4 interacts with Raf-1 and MEK1 in TRAIL-treated CaoV-3 cells by co-immunoprecipitation analysis."
| 1
sparser
"It has been shown that Raf-1 and B-Raf interact with both MEK1 and MEK2."
ERK binds MAP2K1, ARRB2, and RAF1. 1 / 1
| 1
sparser
"Therefore, for the analysis of arrestin-2 interactions with c-Raf1, MEK1, and ERK1/2 we only used ten mutants that demonstrated normal receptor binding ( xref ), indicative of proper folding."
MEK binds HRAS, MAP2K1, and RAF1. 1 / 1
| 1
sparser
"Overexpression of transgenes encoding do forms of Raf-1 [68], p21ras [69], Mek-I [70] or both p21ras and Mek-1 [71'] inhibited thymocyre development past the DP stage."
| 1
sparser
"MP-1 selectively interacts with ERK-1 and MEK-1 but not Raf-1, on the surface of cytoplasmic late endosomes and lysosomes [80] ."
| 1
sparser
"Xu et al ( xref ) and Karandikar et al ( xref ) both showed that MEKK1 binds to C-Raf, MEK1 and ERK2 of the ERK1/2 MAPK signaling module."
| 1
sparser
"Downstream of Pak1, both Raf-1 and Mek-1 were associated with Bam32 independently of SLP-76 expression."
AKT binds GRB10, MAP2K1, and RAF1. 1 / 1
| 1
sparser
"For example, Grb10 interacts with Raf1, MEK1 and Akt ( Jahn et al., 2002 ; Nantel et al., 1998 )."
1 |
hprd
No evidence text available
ERK binds KSR1, MAP2K1, RAF1, and flotillin-1. 1 / 1
| 1
sparser
"We here show that flotillin-1 forms a complex with CRAF, MEK1, ERK, and KSR1 (kinase suppressor of RAS) and that flotillin-1 knockdown leads to a direct inactivation of ERK1/2."
ERK binds PHB2, MAP2K1, PHB, and RAF1. 1 / 1
| 1
sparser
"PHB and PHB2 interact with c-Raf to induce the phosphorylation of ERK1/2 via MEK1 [ xref ]."
| 1
sparser
"Moreover, KSR1 is able to translocate from the cytoplasm to the plasma membrane where it forms a complex with Raf-1, MEK1 and 14-3-3 in the presence of activated Ras, supporting its scaffolding function in the MAPKs pathway [ xref , xref ]."
ERK binds JNK, ERBB2, MAP2K1, and RAF1. 1 / 1
| 1
sparser
"HER2 is a well-known upstream kinase of RAF/MAPK, and, therefore, we examined whether BRACs affected the interaction of HER2 with RAF1, MEK1, ERK1/2, and JNK."
MAP2K1 affects RAF1, and RAS
| 7
RAS binds MAP2K1 and RAF1. 6 / 6
| 6
sparser
"Within the SGZ of the adult brain, we now report elevations in genes associated with both IL-6Rα and gp130, as well as genes associated with downstream signaling through Ras to activate Raf-1 or MAP2K1."
sparser
"Overexpression of dominant-negative forms of Ras , Raf-1 , and MEK1 in transgenic mice results in defective positive but normal negative selection."
sparser
"Through interaction with Raf-1, RKTG changes the localization of Raf-1 from cytoplasm to the Golgi apparatus, blocks EGF-stimulated Raf-1 membrane translocation, and reduces the interaction of Raf-1 with Ras and MEK1."
sparser
"Transient transfection of Pa-4 cells with active forms of Ras, Raf-1, or MEK1 resulted in decreases of occludin mRNA levels, which can be reversed by the MEK inhibitor PD98059."
sparser
"The binding of PEA-15 was specific to ERK among the components of the ERK MAP kinase pathway, since PEA-15 failed to interact with Ras, Raf1, or MEK1 ."
sparser
"Consistent with the preferential binding of MEK-1 to RAS:RAF-1, the basal activity of MEK-1 in v-ras-transformed cells was found to be elevated sixfold, whereas MEK-2 was elevated only twofold, suggesting that the RAS signalling pathway favors MEK-1 activation."
| 1
sparser
"Additionally, increased levels of Raf-1 are associated with Ras and MEK1 in Alzheimer's disease as evidenced by its coimmunoprecipitation with Ras and Mek1, respectively."
MAP2K1 affects MAPK1, and RAF1
| 5
| 5
sparser
"To identify arrestin-2 elements involved in the binding of c-Raf1, MEK1, and ERK2 we generated 22 mutants in which residues conserved between non-visual arrestins on the non-receptor-binding surface were replaced with alanines individually or in groups ( xref , xref )."
sparser
"Expression of PEBP1 results in production of phosphatidylethanolamine-binding protein 1, a protein that in humans has been shown to interact with C-Raf, MAP2K1 and MAPK1 [ xref ]."
sparser
"MEKK1, an important regulator in stress- and cytokine-induced JNK/SAPK pathways, has also been implicated in the activation of ERK1/2 (Minden et al., 1994; Xu et al., 1996; Yujiri et al., 1998) and [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"The effect of arrestin-2 and arrestin-3 binding to the β2-adrenergic receptor on its interactions with c-Raf1, MEK1, and ERK2 in cellular environment."
sparser
"We examined the importance of this binding site in the feedback phosphorylation of MEK1 on Thr(292) and Thr(386) by ERK2, the phosphorylation and activation of ERK2 by MEK1, and the interaction of MEK1 with ERK2 and Raf-1."
MAP2K1 affects PEBP1, and RAF1
| 3
| 3
sparser
"It has been shown that RKIP binds to Raf-1 and MEK1 at overlapping sites [14] ."
sparser
"In the presence of RKIP, RAF1 cannot bind MEK1 ( xref )."
sparser
"A yeast two-hybrid assay screen of a human T cell library was used to identify proteins that bound to Raf-1 kinase binding domains. xref The protein identified was designated RKIP, whose sequence was analogous to the human and monkey 23 kDa protein PEBP. xref Using anti-RKIP antibody, antisense RKIP and sense RKIP expression vectors, Yeung et al. discovered that RKIP could bind Raf-1 and MEK-1, and weakly bind to ERK-2, interfering with MEK phosphorylation and activation by Raf-1."
BRAF affects MAP2K1, and RAF1
| 3
BRAF binds MAP2K1 and RAF1. 2 / 2
| 2
sparser
"When bound to trametinib, the dissociation constants for the interaction of MEK1 with either BRAF or CRAF kinase were 0.101 μM and 0.107 μM, respectively."
sparser
"Moreover, the overexpression of MEK1(3A) failed to block endogenous MEK1 activation, although MEK1(3A) formed complexes with both c-Raf and B-Raf as well as p42/p44 MAPK."
BRAF binds BRAF, MAP2K1, and RAF1. 1 / 1
| 1
sparser
"For example, high expression levels of p-B-Raf p-RAF1 and p-MEK1 were associated with BRAF mutations; high expression levels of p-EGFR, p-SHP-2 and p-Stat3 were linked to EGFR mutations; and high levels of p-Akt or p-HER2 were associated with PIK3CA mutations and ERBB2 mutations, respectively."
MAP2K1 affects PEBP4, and RAF1
| 2
| 2
sparser
"Wang et al [5] have shown that PEBP4 interacts with Raf-1 and MEK1 and attenuates activation of MEK1."
sparser
"Co-precipitation and in vitro protein binding assay demonstrated that hPEBP4 interacts with Raf-1 and MEK1."
MAP2K1 affects MAP2K3, MAP2K7, MAP3K1, MAPK1, MAPK8, RAF1, and RAS
| 2
sparser
"Furthermore, protein kinase C inhibitor blocks the PMA dependent activation of MAO B. Cotransfection of the MAO B promoter with dominant negative forms of Ras, Raf-1, MEKK1, MEK1, MEK3, MEK7, ERK2, JN[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"Furthermore, protein kinase C inhibitor blocks the PMA-dependent activation of MAO B. Co-transfection of the MAO B promoter with dominant negative forms of Ras, Raf-1, MEKK1, MEK1, MEK3, MEK7, ERK2, JNK1, and p38/RK inhibit the PMA-dependent activation of the MAO B promoter."
ERK affects MAP2K1, and RAF1
| 2
ERK binds MAP2K1 and RAF1. 2 / 2
| 2
sparser
"β-arrestin2 scaffolds appear to act in ERK activation by mediating binding of ERK1/2 and Raf1 and MEK-1."
sparser
"MEK1 but not MEK2 forms a signaling complex with Raf-1, suggesting that in some cells the Raf-1 signaling pathway preferentially activates ERKs via MEK1 ( xref )."
ERK affects MAP2K1, PEBP1, and RAF1
| 2
ERK binds MAP2K1, PEBP1, and RAF1. 2 / 2
| 2
sparser
"This mode of action bears striking similarities to the interactions of RKIP with Raf-1 and MEK1 in the MAPK pathway."
sparser
"This mode of action bears striking similarities to the interaction of RKIP with Raf-1 and MEK1 in the MAPK pathway."
BRAF affects CBL, GNL3, KRAS, MAP2K1, NRAS, PTPN11, RAF1, SHOC2, and SOS1
| 2
sparser
"NS is associated with PTPN11 , SOS1 , KRAS , NRAS , RAF1 , BRAF , SHOC2 , MEK1 and CBL gene mutations [ xref - xref ], LS with PTPN11, RAF1 and BRAF gene mutations [ xref , xref , xref , xref ], NS/LAH with SHOC2 gene mutations [ xref ], CFCS with KRAS , BRAF , MEK1 and MEK2 gene mutations [ xref , xref ], CS with HRAS gene mutations [ xref ]."
sparser
"NS is associated with mutations in PTPN11 , SOS1 , KRAS , NRAS , RAF1 , BRAF , SHOC2 , MEK1 and CBL [ xref , xref , xref , xref , xref , xref , xref , xref ], CS with mutations in HRAS [ xref ], CFCS with mutations in KRAS , BRAF , MEK1 and MEK2 [ xref , xref ], NS/LAH with mutations in SHOC2 [ xref ], LS with mutations in PTPN11 , RAF1 and BRAF [ xref , xref , xref ]."
MAP2K1 affects RAF1, and arrestin
| 1
MAP2K1 binds RAF1 and arrestin. 1 / 1
| 1
sparser
"Arrestins that do not bind MEK1 or c-Raf1."
MAP2K1 affects RAF1, and T292
| 1
MAP2K1 binds RAF1 and T292. 1 / 1
| 1
reach
"Frost and co-workers showed that MEK1 with mutations of T292 and S298 to alanine bound c-Raf less efficiently than did wild-type MEK1."
MAP2K1 affects RAF1, and RHO
| 1
RHO binds MAP2K1 and RAF1. 1 / 1
| 1
sparser
"Intriguingly, it is the activation of the core pathway (RAF/MEK/ERK) that generates the phosphorylated forms of RAF1 and MEK1 required for the cross-talk with the RHO and with PI3K pathway, respective[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
MAP2K1 affects PLA2G4, and RAF1
| 1
| 1
sparser
"Sustained activation of MAPK3/1 beyond 3 h was independent of EGFR kinase activity but dependent on PKC activity, which appeared to form a self-sustaining MAPK3/1 activation loop by activating RAF1, MAP2K1, and PLA2G4 (phospholipase A2 [all cytosolic isozymes])."
MAP2K1 affects PEBP4, RAF1, and TNFSF10
| 1
| 1
sparser
"We found that endogenous hPEBP4 interacts with Raf-1 and MEK1 in TRAIL-treated CaoV-3 cells by co-immunoprecipitation analysis."
MAP2K1 affects NEK10, and RAF1
| 1
| 1
sparser
"Nek10 physically associated with Raf-1 and MEK1 in a Raf-1-dependent manner, and the formation of this complex was necessary for Nek10-mediated MEK1 activation."
MAP2K1 affects MAPK3, PITRM1, and RAF1
| 1
| 1
sparser
"MP-1 selectively interacts with ERK-1 and MEK-1 but not Raf-1, on the surface of cytoplasmic late endosomes and lysosomes [80] ."
MAP2K1 affects MAPK, RAF1, RAS, and p14_3_3
| 1
| 1
sparser
"Moreover, KSR1 is able to translocate from the cytoplasm to the plasma membrane where it forms a complex with Raf-1, MEK1 and 14-3-3 in the presence of activated Ras, supporting its scaffolding function in the MAPKs pathway [ xref , xref ]."
MAP2K1 affects MAP3K1, MAPK1, and RAF1
| 1
| 1
sparser
"Xu et al ( xref ) and Karandikar et al ( xref ) both showed that MEKK1 binds to C-Raf, MEK1 and ERK2 of the ERK1/2 MAPK signaling module."
MAP2K1 affects MAP2K4, MAP3K5, MAPK1, MAPK10, RAF1, and arrestin
| 1
MAP2K1 binds MAP2K4, MAP3K5, MAPK1, MAPK10, RAF1, and arrestin. 1 / 1
| 1
sparser
"Subsequent studies showed that free arrestins also bind ASK1, JNK3, MKK4, c-Raf1, MEK1, and ERK2 (10–12, xref – xref )."
HRAS affects MAP2K1, and RAF1
| 1
HRAS binds MAP2K1 and RAF1. 1 / 1
| 1
sparser
"In contrast, expression of constitutively active forms of H-Ras, Raf1, or Mek1, in mouse as well as human skin, promotes proliferation and hyperplasia ( xref , xref )."
HRAS affects MAP2K1, MEK, and RAF1
| 1
MEK binds HRAS, MAP2K1, and RAF1. 1 / 1
| 1
sparser
"Overexpression of transgenes encoding do forms of Raf-1 [68], p21ras [69], Mek-I [70] or both p21ras and Mek-1 [71'] inhibited thymocyre development past the DP stage."
GRB10 affects MAP2K1, and RAF1
| 1
| 1
sparser
"The SH2 domain of GRB10 binds to receptor tyrosine kinases and, in a phosphotyrosine-independent manner, GRB10 binds to RAF-1 and MEK1 (Ref. [31] )."
ERK affects MAP2K1, PHB, PHB2, and RAF1
| 1
ERK binds PHB2, MAP2K1, PHB, and RAF1. 1 / 1
| 1
sparser
"PHB and PHB2 interact with c-Raf to induce the phosphorylation of ERK1/2 via MEK1 [ xref ]."
ERK affects MAP2K1, MAPK1, and RAF1
| 1
ERK binds MAP2K1, MAPK1, and RAF1. 1 / 1
| 1
sparser
"It also constitutively interacts with Raf-1, MEK1, and ERK2 to facilitate stimulus-induced activation of ERK1/2 ( xref , xref , xref )."
ERK affects KSR1, MAP2K1, RAF1, and flotillin-1
| 1
ERK binds KSR1, MAP2K1, RAF1, and flotillin-1. 1 / 1
| 1
sparser
"We here show that flotillin-1 forms a complex with CRAF, MEK1, ERK, and KSR1 (kinase suppressor of RAS) and that flotillin-1 knockdown leads to a direct inactivation of ERK1/2."
ERK affects KSR1, MAP2K1, MAPK1, RAF1, and flotillin-1
| 1
ERK binds KSR1, MAP2K1, MAPK1, RAF1, and flotillin-1. 1 / 1
| 1
sparser
"Later on, flotillin-1 binds the core components of the MAPK cascade C-Raf, MEK1 and ERK2 simultaneously and independently of KSR1 ( xref )."
ERBB2 affects ERK, JNK, MAP2K1, and RAF1
| 1
ERK binds JNK, ERBB2, MAP2K1, and RAF1. 1 / 1
| 1
sparser
"HER2 is a well-known upstream kinase of RAF/MAPK, and, therefore, we examined whether BRACs affected the interaction of HER2 with RAF1, MEK1, ERK1/2, and JNK."
DAPP1 affects LCP2, MAP2K1, and RAF1
| 1
| 1
sparser
"Downstream of Pak1, both Raf-1 and Mek-1 were associated with Bam32 independently of SLP-76 expression."
CDC42 affects ERK, MAP2K1, PAK1, PAK3, RAC1, and RAF1
| 1
| 1
sparser
"These findings may be explained by the previous findings that Rac1 and Cdc42 can interact with Raf-1 in a synergistic manner to activate ERK via pathways that involve phosphorylation of both MEK1 by PAK1 and Raf-1 by PAK-3 [ xref , xref ]."
CDC37 affects HSF1, MAP2K1, and RAF1
| 1
| 1
sparser
"The substrates Raf-1, HSF1, Cdc37, and MEK1 interacted with both Hsp90alpha and Hsp90beta, and the relative patterns of these interactions were not affected by heat shock."
BRAF affects MAP2K1, MAP2K2, and RAF1
| 1
| 1
sparser
"It has been shown that Raf-1 and B-Raf interact with both MEK1 and MEK2."
ARRB2 affects ERK, MAP2K1, and RAF1
| 1
ERK binds MAP2K1, ARRB2, and RAF1. 1 / 1
| 1
sparser
"Therefore, for the analysis of arrestin-2 interactions with c-Raf1, MEK1, and ERK1/2 we only used ten mutants that demonstrated normal receptor binding ( xref ), indicative of proper folding."
AKT affects GRB10, MAP2K1, and RAF1
| 1
AKT binds GRB10, MAP2K1, and RAF1. 1 / 1
| 1
sparser
"For example, Grb10 interacts with Raf1, MEK1 and Akt ( Jahn et al., 2002 ; Nantel et al., 1998 )."
AGTR1 affects ARRB2, MAP2K1, MAPK1, and RAF1
1 |
1 |
hprd
No evidence text available