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phosphosite cbn pc11 biopax bel_lc signor biogrid tas lincs_drug hprd trrust | geneways tees isi trips rlimsp medscan sparser reach
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RAF1 affects KRAS
2 9 1 | 39 26
2 9 1 | 28 24
sparser
"Oncogenic KRAS binds and activates CRAF more efficiently and mediates KRAS signaling to ERK kinase in lung cancer cells, thus fulfilling an important role as an anti-apoptotic protein independent of BRAF ( xref , xref )."
reach
"Thus, the comparison of the effect of K-Ras and CAAX mutated K-Ras protein K-RasC185S on the cellular localization of Raf1 may provide useful information regarding the cellular interaction between K-Ras and Raf1."
reach
"Following the transition to late endosomes, Raf1 bound K-Ras activates the protein complex that include adaptors p14 and MP1 that in turn recruits and binds MEK1, thereby facilitating ERK1/2 entry into the nucleus."
sparser
"Also, phloroglucinol effectively decreased the active form of KRAS that could interact with RAF-1 (Fig. xref )."
reach
"Oncogenic KRAS binds and activates CRAF more efficiently and mediates KRAS signaling to ERK kinase in lung cancer cells, thus fulfilling an important role as an anti-apoptotic protein independent of BRAF."
reach
"In other words, the Raf1 and K-Ras complexes will not lose their positioning in the cell membrane while association occurs in the cell membrane, and unless they are separated."
hprd
No evidence text available
sparser
"Binding of prenylated KRAS to nanodisc, and subsequent binding of the CRAF-RBD to KRAS, was shown to be dependent on PS [ xref ] and interfering with the PS content of the plasma membrane was shown to mislocalize KRAS and impact signaling [ xref , xref ]."
reach
"Next, we determined whether everolimus treatment enhances the interaction of mt K-Ras and c-Raf for the Ras-Raf-ERK activation."
reach
"In contrast, an apparent cytosolic distribution pattern was observed in cells co-transfected with mcerulean-Raf1 and EGFP-K-RasC185S, suggesting that the membrane localization of K-Ras and Raf1 complexes is not entirely dependent on K-Ras, and that other factors, such as the irreversible conformation formed between K-Ras and Raf1 may play a role."
HRAS binds KRAS and RAF1. 3 / 3
| 3
sparser
"Kobe0065, and a less potent analog (Kobe2602) identified by similarity searching, inhibited HRAS- and KRAS:RAF-RBD binding in vitro ."
sparser
"KRAS mutations, unlike mutations of RAF1 and HRAS, are rarely associated with HCM."
sparser
"Recently, xref , xref reported that a calmodulin inhibitor induced association of HRas and KRas with Raf1 in COS-1 cells."
MEK binds KRAS and RAF1. 2 / 2
| 2
sparser
"Indeed, PD0325901 increased the interaction between MEK1/2 and C-Raf in KRAS mutant tumor cells, although not in BRAF mutant tumor cells xref ."
sparser
"Our results indicate that TLN-4601 decreases activated K-Ras (K-Ras-GTP) and Raf-1, which are associated with the inhibition of K-Ras-induced phosphorylation of MEK kinase."
BRAF binds KRAS and RAF1. 2 / 2
| 2
sparser
"It also strongly stimulated KRASBRAF and KRASCRAF interactions and inhibited RAF intramolecular interaction with potencies similar to GDC-0879 (Fig.  xref , f; Supplementary Fig.  xref )."
sparser
"Consistent with this, the interaction of KRAS G12V with BRAF and CRAF gatekeeper mutants was resistant to GDC-0879 (type I), but not to AZ-628 (type II) in BRET dose-response experiments (Fig.  xref and Supplementary Fig.  xref )."
Mutated KRAS binds RAF1. 1 / 1
| 1
reach
"By phosphorylating mutant Kras bound cRaf, growth factors can potently engage the ras-Raf signaling cascade, which deactivates slowly due to decreased GTPase activity of mutant Kras [XREF_BIBR, XREF_BIBR]."
KRAS-G12V binds RAF1. 1 / 1
| 1
reach
"Further, BRET analysis revealed that NS1 disrupts CRAF interaction with K-RAS (G12V) but not N-RAS (G12V) (XREF_SUPPLEMENTARY)."
G_12 binds KRAS and RAF1. 1 / 1
| 1
sparser
"We also tested the effect of compound Ch-3 in the BRET assay using five different full-length KRAS G12 mutations interacting with full-length CRAF ( xref : G12A, G12C, G12R, and G12V) or CRAF RAS-binding domain (RBD) ( xref : G12D)."
KRAS binds NRAS and RAF1. 1 / 1
| 1
sparser
"Interactions of H-Ras with PI3Kα, and with scaffolding protein galectin-1 were observed to be orientation sensitive, and the N-Ras and K-Ras interactions with the C-Raf-binding domain are differentially affected by mutations in the switch elements which relate to Ras orientation ( xref ; xref )."
PI3K binds KRAS, RAF1, and G12V/K147L. 1 / 1
| 1
sparser
"Although non-ubiquitinated G12V-KRas and G12V/K147L-KRas equally interacted with PI3K and Raf1, ubiquitinated G12V/K147L reduced its binding to PI3K but not Raf kinase ( xref )."
RAF binds BRAF, KRAS, ARAF, and RAF1. 1 / 1
| 1
sparser
"Activated KRAS binds and activates RAF family kinases, RAF1, BRAF, and ARAF [ xref ]."
KRAS affects RAF1
2 9 1 | 39 26
2 9 1 | 28 24
sparser
"Oncogenic KRAS binds and activates CRAF more efficiently and mediates KRAS signaling to ERK kinase in lung cancer cells, thus fulfilling an important role as an anti-apoptotic protein independent of BRAF ( xref , xref )."
reach
"Thus, the comparison of the effect of K-Ras and CAAX mutated K-Ras protein K-RasC185S on the cellular localization of Raf1 may provide useful information regarding the cellular interaction between K-Ras and Raf1."
reach
"Following the transition to late endosomes, Raf1 bound K-Ras activates the protein complex that include adaptors p14 and MP1 that in turn recruits and binds MEK1, thereby facilitating ERK1/2 entry into the nucleus."
sparser
"Also, phloroglucinol effectively decreased the active form of KRAS that could interact with RAF-1 (Fig. xref )."
reach
"Oncogenic KRAS binds and activates CRAF more efficiently and mediates KRAS signaling to ERK kinase in lung cancer cells, thus fulfilling an important role as an anti-apoptotic protein independent of BRAF."
reach
"In other words, the Raf1 and K-Ras complexes will not lose their positioning in the cell membrane while association occurs in the cell membrane, and unless they are separated."
hprd
No evidence text available
sparser
"Binding of prenylated KRAS to nanodisc, and subsequent binding of the CRAF-RBD to KRAS, was shown to be dependent on PS [ xref ] and interfering with the PS content of the plasma membrane was shown to mislocalize KRAS and impact signaling [ xref , xref ]."
reach
"Next, we determined whether everolimus treatment enhances the interaction of mt K-Ras and c-Raf for the Ras-Raf-ERK activation."
reach
"In contrast, an apparent cytosolic distribution pattern was observed in cells co-transfected with mcerulean-Raf1 and EGFP-K-RasC185S, suggesting that the membrane localization of K-Ras and Raf1 complexes is not entirely dependent on K-Ras, and that other factors, such as the irreversible conformation formed between K-Ras and Raf1 may play a role."
HRAS binds KRAS and RAF1. 3 / 3
| 3
sparser
"Kobe0065, and a less potent analog (Kobe2602) identified by similarity searching, inhibited HRAS- and KRAS:RAF-RBD binding in vitro ."
sparser
"KRAS mutations, unlike mutations of RAF1 and HRAS, are rarely associated with HCM."
sparser
"Recently, xref , xref reported that a calmodulin inhibitor induced association of HRas and KRas with Raf1 in COS-1 cells."
MEK binds KRAS and RAF1. 2 / 2
| 2
sparser
"Indeed, PD0325901 increased the interaction between MEK1/2 and C-Raf in KRAS mutant tumor cells, although not in BRAF mutant tumor cells xref ."
sparser
"Our results indicate that TLN-4601 decreases activated K-Ras (K-Ras-GTP) and Raf-1, which are associated with the inhibition of K-Ras-induced phosphorylation of MEK kinase."
BRAF binds KRAS and RAF1. 2 / 2
| 2
sparser
"It also strongly stimulated KRASBRAF and KRASCRAF interactions and inhibited RAF intramolecular interaction with potencies similar to GDC-0879 (Fig.  xref , f; Supplementary Fig.  xref )."
sparser
"Consistent with this, the interaction of KRAS G12V with BRAF and CRAF gatekeeper mutants was resistant to GDC-0879 (type I), but not to AZ-628 (type II) in BRET dose-response experiments (Fig.  xref and Supplementary Fig.  xref )."
Mutated KRAS binds RAF1. 1 / 1
| 1
reach
"By phosphorylating mutant Kras bound cRaf, growth factors can potently engage the ras-Raf signaling cascade, which deactivates slowly due to decreased GTPase activity of mutant Kras [XREF_BIBR, XREF_BIBR]."
KRAS-G12V binds RAF1. 1 / 1
| 1
reach
"Further, BRET analysis revealed that NS1 disrupts CRAF interaction with K-RAS (G12V) but not N-RAS (G12V) (XREF_SUPPLEMENTARY)."
G_12 binds KRAS and RAF1. 1 / 1
| 1
sparser
"We also tested the effect of compound Ch-3 in the BRET assay using five different full-length KRAS G12 mutations interacting with full-length CRAF ( xref : G12A, G12C, G12R, and G12V) or CRAF RAS-binding domain (RBD) ( xref : G12D)."
KRAS binds NRAS and RAF1. 1 / 1
| 1
sparser
"Interactions of H-Ras with PI3Kα, and with scaffolding protein galectin-1 were observed to be orientation sensitive, and the N-Ras and K-Ras interactions with the C-Raf-binding domain are differentially affected by mutations in the switch elements which relate to Ras orientation ( xref ; xref )."
PI3K binds KRAS, RAF1, and G12V/K147L. 1 / 1
| 1
sparser
"Although non-ubiquitinated G12V-KRas and G12V/K147L-KRas equally interacted with PI3K and Raf1, ubiquitinated G12V/K147L reduced its binding to PI3K but not Raf kinase ( xref )."
RAF binds BRAF, KRAS, ARAF, and RAF1. 1 / 1
| 1
sparser
"Activated KRAS binds and activates RAF family kinases, RAF1, BRAF, and ARAF [ xref ]."
HRAS affects KRAS, and RAF1
| 3
HRAS binds KRAS and RAF1. 3 / 3
| 3
sparser
"Kobe0065, and a less potent analog (Kobe2602) identified by similarity searching, inhibited HRAS- and KRAS:RAF-RBD binding in vitro ."
sparser
"KRAS mutations, unlike mutations of RAF1 and HRAS, are rarely associated with HCM."
sparser
"Recently, xref , xref reported that a calmodulin inhibitor induced association of HRas and KRas with Raf1 in COS-1 cells."
KRAS affects MEK, and RAF1
| 2
MEK binds KRAS and RAF1. 2 / 2
| 2
sparser
"Indeed, PD0325901 increased the interaction between MEK1/2 and C-Raf in KRAS mutant tumor cells, although not in BRAF mutant tumor cells xref ."
sparser
"Our results indicate that TLN-4601 decreases activated K-Ras (K-Ras-GTP) and Raf-1, which are associated with the inhibition of K-Ras-induced phosphorylation of MEK kinase."
BRAF affects KRAS, and RAF1
| 2
BRAF binds KRAS and RAF1. 2 / 2
| 2
sparser
"It also strongly stimulated KRASBRAF and KRASCRAF interactions and inhibited RAF intramolecular interaction with potencies similar to GDC-0879 (Fig.  xref , f; Supplementary Fig.  xref )."
sparser
"Consistent with this, the interaction of KRAS G12V with BRAF and CRAF gatekeeper mutants was resistant to GDC-0879 (type I), but not to AZ-628 (type II) in BRET dose-response experiments (Fig.  xref and Supplementary Fig.  xref )."
BRAF affects CBL, GNL3, KRAS, MAP2K1, NRAS, PTPN11, RAF1, SHOC2, and SOS1
| 2
sparser
"NS is associated with PTPN11 , SOS1 , KRAS , NRAS , RAF1 , BRAF , SHOC2 , MEK1 and CBL gene mutations [ xref - xref ], LS with PTPN11, RAF1 and BRAF gene mutations [ xref , xref , xref , xref ], NS/LAH with SHOC2 gene mutations [ xref ], CFCS with KRAS , BRAF , MEK1 and MEK2 gene mutations [ xref , xref ], CS with HRAS gene mutations [ xref ]."
sparser
"NS is associated with mutations in PTPN11 , SOS1 , KRAS , NRAS , RAF1 , BRAF , SHOC2 , MEK1 and CBL [ xref , xref , xref , xref , xref , xref , xref , xref ], CS with mutations in HRAS [ xref ], CFCS with mutations in KRAS , BRAF , MEK1 and MEK2 [ xref , xref ], NS/LAH with mutations in SHOC2 [ xref ], LS with mutations in PTPN11 , RAF1 and BRAF [ xref , xref , xref ]."
KRAS affects NRAS, and RAF1
| 1
KRAS binds NRAS and RAF1. 1 / 1
| 1
sparser
"Interactions of H-Ras with PI3Kα, and with scaffolding protein galectin-1 were observed to be orientation sensitive, and the N-Ras and K-Ras interactions with the C-Raf-binding domain are differentially affected by mutations in the switch elements which relate to Ras orientation ( xref ; xref )."
HRAS affects KRAS, NRAS, PIK3CA, RAF1, RAS, and RRAS2
| 1
| 1
sparser
"Equilibrium dissociation constants were determined for the binding of HRAS, KRAS, NRAS, RRAS1 and RRAS2 to both the RAS binding (RB) domain of CRAF and PI3Kα, and the RAS association (RA) domain of RASSF5, RALGDS and PLCε, respectively, using fluorescence polarization."
G_12 affects KRAS, and RAF1
| 1
G_12 binds KRAS and RAF1. 1 / 1
| 1
sparser
"We also tested the effect of compound Ch-3 in the BRET assay using five different full-length KRAS G12 mutations interacting with full-length CRAF ( xref : G12A, G12C, G12R, and G12V) or CRAF RAS-binding domain (RBD) ( xref : G12D)."
G12V/K147L affects KRAS, PI3K, and RAF1
| 1
PI3K binds KRAS, RAF1, and G12V/K147L. 1 / 1
| 1
sparser
"Although non-ubiquitinated G12V-KRas and G12V/K147L-KRas equally interacted with PI3K and Raf1, ubiquitinated G12V/K147L reduced its binding to PI3K but not Raf kinase ( xref )."
ARAF affects BRAF, KRAS, RAF, and RAF1
| 1
RAF binds BRAF, KRAS, ARAF, and RAF1. 1 / 1
| 1
sparser
"Activated KRAS binds and activates RAF family kinases, RAF1, BRAF, and ARAF [ xref ]."
A2ML1 affects CBL, GNL3, KAT6B, KRAS, LZTR1, MAP3K8, NRAS, PTPN11, RAF1, RASA2, RIT1, RRAS, SHOC2, SOS1, SOS2, and SPRY1
| 1
sparser
"For example, NS is associated with mutations in A2ML1 , CBL , KRAS , LZTR1 , MAP3K8 , MYST4 , NRAS , PTPN11 , RAF1 , RASA2 , RRAS , RIT1 , SHOC2 , SOS1 , SOS2 and SPRY1 , whereas mutations in HRAS and SPRED1 have been described for CS and Legius syndrome, respectively ( xref ; xref , xref )."