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phosphosite cbn pc11 biopax bel_lc signor biogrid tas lincs_drug hprd trrust | geneways tees isi trips rlimsp medscan sparser reach
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SOS1 affects GRB2
10 35 7 | 2 253 183
5 35 6 | 2 169 174
sparser
"Two Grb2 molecules can bind to SOS1 to form a Grb2-SOS1-Grb2 dimer, which can bridge two tyrosine phosphorylated LAT molecules."
reach
"Stoichiometry of Grb2 and Sos1 complexes at cellular concentrations."
sparser
"A similar inverse relationship between p66shc expression and grb2sos1 binding was observed in human embryonic kidney (HEK) 293 cells ( xref )."
reach
"Thus, the interaction between Sos-1 and Grb2 is dynamically regulated by RTK and might contribute to the rapid, but transient, activation of Ras after RTK stimulation (XREF_FIG)."
biogrid
No evidence text available
sparser
"To test the extent to which the formation of Grb2-Sos1 complex with a 2:1 stoichiometry may be influenced by steric hindrance, we next measured the binding of full-length Grb2 to various single, double and triple mutant constructs of the PR domain, mutated with respect to one or more of the S1–S4 sites ( xref and xref )."
reach
"Grb2 binds to both the Sos1 and Gab1 peptides with a 1:1 stoichiometry."
sparser
"In a Src-dependent but Syk-independent manner, phosphorylated-Dok3 translocated from the cytoplasm to the plasma membrane where it associated with Grb2 and Sos1 which prevented activation of the RAS-ERK pathway (Fig.  xref ) [ xref ]."
SOS1 binds GAB1 and GRB2. 10 / 16
| 16
sparser
"While we have relied here on short peptides to mimic Sos1 and Gab1, due largely to inherent difficulties associated with isolation and purification of full-length Sos1 and Gab1 proteins, it should be noted that these peptides suffice par excellence for studying the binding of Sos1 and Gab1 to Grb2 using biophysical methods."
sparser
"As shown in xref , it is clearly evident that the stoichiometries and energetics of binding of Sos1 and Gab1 peptides to Grb2 in Tris buffer versus Phosphate buffer are virtually indistinguishable."
sparser
"In light of these considerations, one would expect the binding of Sos1 and Gab1 peptides to full-length Grb2 with stoichiometries of 2:1 and 1:1, respectively."
sparser
"Although efforts by others and us directed at the determination of X-ray or NMR structures of full-length Grb2 bound to Sos1 or Gab1 constructs of any sort over the past decade or so have met no success, presumably due to the transient nature of Sos1-Grb2-Gab1 complex, we have nonetheless made an attempt here to create a crude 3D structural model of how the binding of one molecule of Sos1 to the nSH3 domain may allosterically induce a conformational change within Grb2 such that the loading of a second molecule of Sos1 onto the cSH3 domain is blocked and, in so doing, allowing Gab1 access to the cSH3 domain in an exclusively non-competitive manner to generate the Sos1-Grb2-Gab1 ternary complex ( xref )."
sparser
"Although these CD measurements are of highly qualitative nature and thus do not provide the physical basis of how the binding of Sos1 to the nSH3 domain may trigger a conformational change within Grb2 such that the nSH3 domain is only accessible to Gab1, the fact that Grb2 appears to be a highly flexible molecule by virtue of its ability to undergo discernable secondary and tertiary structural changes upon binding to Sos1 and Gab1 peptides nonetheless supports the notion that allostery is likely to play a central role in mediating communication between the nSH3 and cSH3 domains so as to allow the assembly of Sos1-Grb2-Gab1 ternary signaling complex in a non-competitive manner."
sparser
"On the contrary, our previous work has shown that the formation of Sos1-Grb2-Gab1 ternary complex is under tight allosteric control [ xref ]."
sparser
"Grb2 binds to both the Sos1 and Gab1 peptides with a 1:1 stoichiometry."
sparser
"Given that the formation of Sos1-Grb2-Gab1 ternary signaling complex proceeds in a non-competitive manner [ xref ], the most straightforward interpretation of these salient observations is that the Sos1 peptide binds only to the nSH3 domain but not the cSH3 domain, while the Gab1 peptide binds only to the cSH3 domain in the context of full-length Grb2."
sparser
"FRS2 was originally discovered as a docking site for coordinated assembly of a multiprotein complex that includes GRB2, GAB1, and SOS1, and serves a critical role in the FGFR signaling pathway (Sato and Gotoh xref )."
sparser
"Importantly, the formation of both the Grb2-Sos1 and Grb2-Gab1 binary complexes is governed by a stoichiometry of 2:1, whereby the respective SH3 domains within Grb2 homodimer bind to Sos1 and Gab1 via multivalent interactions."
SOS1 binds GRB2 and PGR. 7 / 7
| 7
sparser
"To understand stoichiometry and the underlying thermodynamic forces driving the formation of Grb2-Sos1 signaling complex, we next measured the binding of full-length Grb2 to the PR domain of Sos1 using ITC (representative data are shown in xref and all data summarized in xref )."
sparser
"Structural basis of the binding of Grb2 to the PR domain of Sos1 as two independent monomers versus a homodimer."
sparser
"Simply put, these observations strongly argue that the Grb2 homodimer bound to PR domain of Sos1 is structurally more stable than Grb2 monomers."
sparser
"MD simulations support the binding of Grb2 to the PR domain of Sos1 as a homodimer in lieu of two independent monomers."
sparser
"These salient observations indicate that the positive cooperativity associated with bivalent binding of Grb2 to the PR domain of Sos1 arises through both the formation of additional intermolecular contacts (favorable enthalpy) as well as burial of additional hydrophobic surfaces (favorable entropy)."
sparser
"In light of these observations, it is thus conceivable that Grb2 could bind to the PR domain of Sos1 either as two independent monomers (Grb2-PR-Grb2), or alternatively as a homodimer ([Grb2] 2 -PR)."
sparser
"Grb2 binds to the PR domain of Sos1 with a 2:1 stoichiometry."
SOS1 binds LAT and GRB2. 7 / 7
| 7
sparser
"Biophysical studies assessing the interactions between LAT, Grb2, and Sos1 have shown that Sos1 has two distinct Grb2-binding sites in its proline-rich region (PRR), and that Sos1 and Grb2 can form a 1:2 complex ( xref )."
sparser
"The exclusion of CD45 was recapitulated in a simpler system in which clusters were formed by pLAT, Grb2, and Sos1 ( xref )."
sparser
"Interestingly, activation of ERK1/2 is delayed relative to protein kinase C, xref suggesting that this alone is not sufficient to activate the MAPK pathway, leading us to speculate that this delay is as a result of assembly of the LAT-Grb2-SOS1 signaling complex."
sparser
"These data suggest an interesting hypothesis whereby multivalent, cooperative interactions between LAT, Grb2, and Sos1 promote LAT oligomerization, whereas recruitment of PLC-γ1–GADS-SLP-76 complexes block Grb2-LAT associations, and therefore limit the overall size of LAT oligomers."
sparser
"Furthermore, biophysical ( xref ) and modeling ( xref ) studies showed that multiple, cooperative interactions between LAT, Grb2, and Sos1 regulate the efficiency of higher-order LAT oligomerization."
sparser
"The existence of multivalent interactions between LAT:Grb2 (1:3) and Sos1:Grb2 (1:2) has led to the hypothesis that Grb2Sos1Grb2 complexes may nucleate LAT oligomerization ( xref )."
sparser
"Based on the multiplicity of Sos1:Grb2 and LAT:Grb2 interactions, it was hypothesized that the Grb2Sos1Grb2 complex could nucleate LAT oligomerization."
SOS1 binds CBL and GRB2. 5 / 5
| 5
sparser
"What the actual contacts are that drive the interaction of GRB2 with either SOS1 or CBL are outside of the scope of this study."
sparser
"Instead, as shown by our studies with the SOS1 scrambled mutant, regions outside of the consensus PxxPxR motifs drive the interaction of the complete proline rich region of SOS1 and CBL with full-length GRB2."
sparser
"In T cells, the SH2 domain of GRB2 binds phosphorylated tyrosines on the adaptor protein LAT and the GRB2 SH3 domains associate with the proline-rich regions of SOS1 and CBL."
sparser
"In contrast, these same PxxPxR motifs were neither necessary nor sufficient for the high affinity interaction of SOS1 or CBL with full-length GRB2 or required for the in vivo recruitment of SOS1 to the plasma membrane in activated T cells."
sparser
"To more fully address this prediction, we utilized quantitative biophysical and imaging techniques to examine the binding of GRB2 to its physiological ligands SOS1 and CBL."
SOS1 binds DOK3 and GRB2. 5 / 5
| 4 1
reach
"DOK3 contributes to the regulation of ERK activation downstream of LPS signaling and the formation of a Grb2, SOS1, and DOK3 complex appears to limit ERK activation."
sparser
"DOK3 was associated with Sos1 and Grb2 in unstimulated wild-type macrophages, but the extent of this association was considerably increased in response to low-dose LPS."
sparser
"Whereas our current studies showed that the association of DOK3 with Sos1 and Grb2 in response to low-dose LPS was required to inhibit ERK activation, at higher concentrations of TLR ligands, including LPS at >100 ng/ml and CpG at 0.5 µM, DOK3 becomes ubiquitinated and degraded ( xref )."
sparser
"These data suggest that the DAP12-dependent tyrosine phosphorylation of DOK3 is required for the association of DOK3 with Grb2 and Sos1."
sparser
"DOK3 contributes to the regulation of ERK activation downstream of LPS signaling and the formation of a Grb2-SOS1-DOK3 complex appears to limit ERK activation."
SOS1 binds EGFR and GRB2. 3 / 3
1 | 2
bel
"In response to stimulation with epidermal growth factor (EGF), the guanine nucleotide exchange factor human SOS1 (hSOS1) promotes the activation of Ras by forming a complex with Grb2 and the human EGF receptor (hEGFR)"
sparser
"Upon EGF stimulation, SOS1 binds to phosphorylated EGFR through the adaptor protein GRB2, which then triggers RAS activation at the plasma membrane ."
sparser
"Although the low-copy-number vector pBT(L) was the best in the case of EGFR-Grb2-Sos1 Y3H interaction, it may also cause reduced bait expression and lowered sensitivity for the detection of weaker protein interactions."
SOS1 binds GRB2 and PTK2. 3 / 3
3 |
bel
"FAK also combines with, and may activate, phosphoinositide 3-OH kinase (PI 3-kinase), either directly or through the Src kinase (13). Finally, there is evidence that Src phosphorylates FAK at Tyr925, creating a binding site for the complex of the adapter Grb2 and Ras guanosine 5'-triphosphate exchange factor mSOS (10). These interactions link FAK to signaling pathways that modify the cytoskeleton and activate mitogen-activated protein kinase (MAPK) cascades (Fig. 3A)."
bel
"FAK also combines with, and may activate, phosphoinositide 3-OH kinase (PI 3-kinase), either directly or through the Src kinase (13). Finally, there is evidence that Src phosphorylates FAK at Tyr925, creating a binding site for the complex of the adapter Grb2 and Ras guanosine 5'-triphosphate exchange factor mSOS (10). These interactions link FAK to signaling pathways that modify the cytoskeleton and activate mitogen-activated protein kinase (MAPK) cascades (Fig. 3A)."
bel
"In the first case, recruited FAK autophosphorylates at 397Y and associates with c-Src or Fyn, which further phosphorylates it at 925Y. Phosphorylated FAK binds to the Grb2-Sos complex and activates the Ras-ERK cascade [60,61]."
SHC binds SOS1 and GRB2. 3 / 3
| 2 1
sparser
"Together, these data suggest that the current model for regulation of p21ras, which proposes a stable association of Shc-grb2-Sos1 complexes at the plasma membrane, may be an oversimplification."
reach
"We also show that the Cbl and EGF receptor complex is predominantly associated with CrkII and is distinct to the Grb2, Shc, and Sos1 complex that associates with the EGF receptor."
sparser
"Ligation of the BCR with anti-IgM antibodies causes an elevation of Ras-GTP levels from about 15% to 25% and coincides with the formation of complexes containing phosphorylated Shc, Grb2 and Sos1 ."
SOS1 binds GRB2 and csh3. 3 / 3
| 3
sparser
"Both the N- and C-SH3 domains of grb2 interact with sos1 ( xref )."
sparser
"As shown in and , the binding of nSH3 and cSH3 domains of Grb2 to Sos1 employs distinct thermodynamic mechanisms."
sparser
"Such orientational constraint imposed upon Grb2-Sos1 assembly by the cSH3 domain coupled with conformational heterogeneity resulting from the promiscuity of nSH3 domain may be an important determinant of regulating the activation of downstream molecules such as Ras."
SOS1 binds GRB2 and GRB2. 3 / 3
| 3
reach
"This is based on the experimental dissociation constant,, for the formation of the Grb2, Sos1, and Grb2 complex from the Grb and Sos1 complex."
reach
"Based on the multiplicity of Sos1 : Grb2 and LAT : Grb2 interactions, it was hypothesized that the Grb2, Sos1, and Grb2 complex could nucleate LAT oligomerization."
reach
"This equation can be rewritten as : (9) or in other words : (10) where is the effective equilibrium constant for the binding of a Grb2 from solution to a Grb2 and Sos1 complex to form a Grb2, Sos1, and Grb2 complex : (11) To make predictions about the binding of Grb2 to the complete polyproline rich domain of Sos1 (1117-1319) we must estimate the values of the unknown equilibrium constants for the binding of the N- and C-SH3 domains of Grb2 to RP on Sos1."
SOS1 binds VAV1 and GRB2. 3 / 3
| 2 1
sparser
"LAT is tyrosine phosphorylated after TCR engagement and recruits molecular complexes formed by the adaptor protein Grb2 and the exchange factors Vav1 and Sos1 ( xref , xref )."
reach
"This adaptor does not contain ITAM domains in its cytoplasmic tail, but it contains one YINM motif, that, upon tyrosine phosphorylation recruits both PI3K and the Grb2, Vav1, and Sos1 complex."
sparser
"Grb2 binds constitutively to the proline rich regions of Sos1 and Vav1 through its N-terminal and C-terminal SH3 domains, respectively."
SHC1 binds SOS1 and GRB2. 2 / 2
1 1 |
hprd
No evidence text available
bel
"In the second case, Fyn or Yes, activated by some b1 and aV integrins, recruit Shc through their SH3 domain and phosphorylates it, thus promoting Shc association with the Grb2-Sos complex [62,63]."
RTK binds SOS1 and GRB2. 2 / 2
| 2
sparser
"The domain organization of SOS1 comprises of a histone fold domain, a Dbl homology (DH)-Pleckstrin homology (PH) domain, a Ras exchanger motif (REM), a CDC25-homology (CDC25-H) domain with GEF activity, and a proline-rich C-terminal region that binds Grb2 and links SOS1 to activated RTKs."
sparser
"Consequently, dn SH3 Grb2 prevents binding of the endogenous Grb2-SOS1 complex to RTKs."
SOS1 binds SOS2, GRB2, and RasGEF. 2 / 2
| 2
sparser
"Once phosphorylated, LAT normally associates with two regulators of Ras activation: PLC-γ1, which activates the RasGEF RasGRP1 via DAG, and Grb2, which directly associates with the RasGEFs Sos1 and Sos2 ( xref )."
sparser
"The RasGEFs Sos1 and Sos2 are constitutively associated with the adaptor Grb2 and are recruited to the membrane where they have basal RasGEF activity via Grb2-LAT interactions (Grb2 binds LAT pY175, pY195, and pY235) ( xref )."
SOS1 binds CBL, GRB2, and PRR. 2 / 2
| 2
sparser
"These observations fit well with previous studies showing that SOS1 peptides bind to individual SH3 domains from GRB2 with affinities ranging from 20μM to 1mM , while the affinity for the binding of f[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"These observations fit well with previous studies showing that SOS1 peptides bind to individual SH3 domains from GRB2 with affinities ranging from 20 µM to 1 mM [ xref – xref ], while the affinity for the binding of full-length GRB2 with full-length SOS1 or the complete PRR of SOS1 and CBL is between 300 and 400 nM ( xref ) and [ xref – xref ]."
SOS1 binds GRB2 and smo-1. 1 / 1
| 1
sparser
"The exact mechanism underlying Grb2 SUMOylation at K 56 recruiting more Sos1 to form SUMO-Grb2-Sos1 complex deserves further study."
SOS1 binds EGF and GRB2. 1 / 1
| 1
sparser
"Whilst the association of Sos1 and Grb2 following EGF stimulation is not cell type specific, we find that it is dependent on cell density and that the response to EGF differs to that induced by NGF."
SOS1 binds GRB2 and C-SH3 domain. 1 / 1
| 1
reach
"Thus, when the Grb2 concentration is much lower than the Sos1 concentration, we expect Grb2 to be doubly bound to Sos1 with its C-SH3 domain bound to the P1 domain."
SOS1 binds GRB2 and sem-5. 1 / 1
| 1
sparser
"Grb2-Sem-5 binds directly to murine Sos1, a Ras exchange factor, through two SH3 domains."
SOS1 binds CSF1R and GRB2. 1 / 1
| 1
sparser
"Fms also formed complexes with Grb2 and Sos1, and neither contained phosphotyrosine."
RRAS binds SOS1 and GRB2. 1 / 1
| 1
sparser
"This method has found wide application in demonstrating interactions between Bcl-2 and R-Ras p23 [45] , SOS1 and GRB2 [46] , Ras and Raf [47,48] , and Raf and 14-3-3 [49] ."
SOS1 binds GRB2 and PRR. 1 / 1
| 1
sparser
"These findings are in contrast to work by McDonald and coworkers, who showed that PxxPxR motifs were critical for the binding of GRB2 to the PRR or SOS1."
sparser
"We hence hypothesized that Cif increases Grb2-SOS1 binding to promote SOS1 activity."
SOS1 binds EPS8 and GRB2. 1 / 1
| 1
sparser
"In contrast, p66shcCH2 and -P47A/P50A led to little or no change in the amounts of grb2 and eps8 bound sos1 ( xref )."
SOS1 binds GRB2 and proline. 1 / 1
| 1
reach
"One LAT molecule is capable of binding up to three Grb2 molecules at a time andtwo Grb2 molecules bind one SOS1 or c-Cbl proline rich domain (XREF_FIG) 33."
SOS1 binds CAV1 and GRB2. 1 / 1
| 1
sparser
"In order to determine whether phospho-caveolin-1 expression sustains RAS signaling, we introduced in to HCT116-Cav-1 AS cells either a full-length caveolin-1 expression construct (WT) or a mutant form of caveolin-1 that is deficient in tyrosine phophorylation at residue 14 (Caveolin-1 Y14F) and assayed for the association of caveolin-1 with the two signaling molecules Grb2 and SOS1."
| 1
sparser
"There are also cases where orthologues in the invertebrates exist, but the interactions have not been observed experimentally as exemplified by the interaction of IIS and MAPK/ERK pathways via GRB2 and SOS1."
SOS1 binds FRS2 and GRB2. 1 / 1
| 1
sparser
"The hetero-oligomer formed between Sprouty1 and Sprouty4 exhibits the most potent inhibitory effect on ERK activation through its highly effective ability to suppress the association of Grb2-Sos1 complex with FRS2."
SOS1 binds GRB2 and E3b1. 1 / 1
| 1
sparser
"Thus, Sos-1 binds either E3b1 or Grb2, in a mutually exclusive fashion, suggesting that two distinct pools of Sos-1 exist in the cells."
SOS1 binds SOS2 and GRB2. 1 / 1
| 1
sparser
"The SH3 domain(s) of Grb2 bind to Sos1 and Sos2 (GEF of the Ras family of GTP-binding protein) ( xref ), SLP-76 ( xref , xref ), and Cbl ( xref , xref , xref )."
SOS1 binds GRB2 and Q07889. 1 / 1
| 1
reach
"A large number of these PPIs are mediated by multiple SLiM classes or SLiM instances, for example, in the interaction between GRB2 (uniprot : P62993) and SOS1 (uniprot : Q07889); SOS1 has seven putative SH3 motifs and GRB2 has two SH3 domains, potentially this can equate to 14 binding interfaces for a single PPI."
SOS1 binds DAB2 and GRB2. 1 / 1
| 1
sparser
"Since the two SH3 domains of Grb2 bind competitively to both Sos1 and Dab2, to their proline-rich motifs, expression levels and phosphorylation of Dab2 and Sos1 modulate the abundance of Sos1/Grb2 complexes."
RAS binds SOS1, GRB2, and PDGFRB. 1 / 1
| 1
sparser
"RasGAP binding to phospho-PDGFRβ causes simultaneous binding of the Grb2-SOS1 complex to Ras that offsets the activation of Ras and reduces the downstream activity of the extracellular signal-regulated protein kinases 1 and 2 (Erk1/2), as shown in vitro xref ."
SHC binds SOS1, EGFR, and GRB2. 1 / 1
| 1
sparser
"In response to calcium influx, Shc, Grb2, and Sos1 inducibly associate with a 180-kDa tyrosine-phosphorylated protein, which was determined to be the epidermal growth factor receptor (EGFR)."
SHC binds SOS1, CHKA, and GRB2. 1 / 1
| 1
sparser
"In our studies, we did not detect the increased association of Shc or Sos1 with Grb2 following expression of CHK (data not shown)."
SOS1 binds VAV1, GRB2, and PPFIA1. 1 / 1
| 1
sparser
"Finally, Kon-Kozlowski et al. ( xref ) have shown that the SH2-domain-containing protein tyrosine phosphatase 1 (SHP-1) associates with Vav1, Grb2, and Sos1."
SOS1 binds EPS8, GRB2, and E3b1. 1 / 1
| 1
sparser
"This model suggests a novel mechanism for Rac1 activation and predicts that the formation of the SNTA1-Grb2-P66shc complex determines the amount of cellular Sos1 that is bound to Grb2 or E3b1-Eps8; in this way, SNTA1 and P66shc modulate Rac1 activation and its downstream cellular functions."
sparser
"On the other hand, Grb2 and Sos1 are associated constitutively in U266 and RPMI8226 MM cells, but phosphorylation of Sos1 is not induced by IL-6."
SOS1 binds EPO, GAB1, and GRB2. 1 / 1
| 1
sparser
"The co-immunoprecipitation experiments revealed an EPO-enhanced association of Gab1 with the Grb2-SOS1 complex and SHP-2 in F-36P cells."
SOS1 binds SH3PXD2A, GRB2, and PRD. 1 / 1
| 1
sparser
"Consequently, Tks5 and Grb2 could simultaneously bind to the Sos1-PRD."
SOS1 binds SPRY1, SPRY4, and GRB2. 1 / 1
| 1
sparser
"They observed that while Spry1 and Spry4 interact with Grb2 and Sos1, respectively, the hetero-oligomer formed by the two exhibits the most potent inhibitory effect on FGF-activated ERK."
SOS1 binds WDR48, GRB2, and IL1R1. 1 / 1
| 1
sparser
"Sos1 and p80 proteins dominantly bind to Grb2 fusion proteins in Chang liver, whereas p100 remarkably associate with Grb2 in HepG2 cells."
SOS1 binds TJP1, CTTN, and GRB2. 1 / 1
| 1
sparser
"In addition, UCS15A was capable of disrupting other typical SH3-mediated protein-protein interactions such as Grb2-Sos1, cortactin-ZO1, as well as atypical SH3-mediated protein-protein interactions such as Grb2-Gab1."
| 1
sparser
"In the light of temporal and spatial control of the association of Tks5 isoforms and Grb2 with Sos1 and dynamin, it will be interesting to elucidate the dependence of complex formation on the phosphor[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
RTK binds SHC, SOS1, and GRB2. 1 / 1
| 1
sparser
"The IGF-I receptor is closely related to the insulin receptor although each has significantly different affinities for its cognate ligand. xref One branch of IGF-I’s mitogenic signaling involves the association of the receptor tyrosine kinase with Shc, Grb2, and Sos-1 to activate Ras and the MAP kinase cascades (Raf, Mek, Erk). xref One of the endpoints of the MAP kinase pathways is the modification of ELK transcription factors, serum response factor (SRF) and AP-1."
SOS1 binds GRB2, csh3, and nSH3. 1 / 1
| 1
sparser
"We have indeed previously shown that salt tightly modulates the binding of nSH3 and cSH3 domains of Grb2 to Sos1 ( xref )."
RTK binds SOS1, EGFR, and GRB2. 1 / 1
| 1
sparser
"In xref , experimental results show that GRB2 recruits SOS1 to the membrane to form GRB2-SOS1 complex upon the activation of EGFR or other RTKs."
ERK binds RAS, SOS1, and GRB2. 1 / 1
| 1
sparser
"Previous reports showed that pDOK3 associates with the cytosolic adaptor protein Grb2 (growth factor receptor–bound protein 2) and the Ras GEF Sos1 (son of sevenless 1) to prevent activation of the Ras-ERK pathway downstream of protein-tyrosine kinases in transfected human embryonic kidney (HEK) 293T cells ( xref )."
GRB2 affects SOS1
10 35 7 | 2 253 183
5 35 6 | 2 169 174
sparser
"Two Grb2 molecules can bind to SOS1 to form a Grb2-SOS1-Grb2 dimer, which can bridge two tyrosine phosphorylated LAT molecules."
reach
"Stoichiometry of Grb2 and Sos1 complexes at cellular concentrations."
sparser
"A similar inverse relationship between p66shc expression and grb2sos1 binding was observed in human embryonic kidney (HEK) 293 cells ( xref )."
reach
"Thus, the interaction between Sos-1 and Grb2 is dynamically regulated by RTK and might contribute to the rapid, but transient, activation of Ras after RTK stimulation (XREF_FIG)."
biogrid
No evidence text available
sparser
"To test the extent to which the formation of Grb2-Sos1 complex with a 2:1 stoichiometry may be influenced by steric hindrance, we next measured the binding of full-length Grb2 to various single, double and triple mutant constructs of the PR domain, mutated with respect to one or more of the S1–S4 sites ( xref and xref )."
reach
"Grb2 binds to both the Sos1 and Gab1 peptides with a 1:1 stoichiometry."
sparser
"In a Src-dependent but Syk-independent manner, phosphorylated-Dok3 translocated from the cytoplasm to the plasma membrane where it associated with Grb2 and Sos1 which prevented activation of the RAS-ERK pathway (Fig.  xref ) [ xref ]."
SOS1 binds GAB1 and GRB2. 10 / 16
| 16
sparser
"While we have relied here on short peptides to mimic Sos1 and Gab1, due largely to inherent difficulties associated with isolation and purification of full-length Sos1 and Gab1 proteins, it should be noted that these peptides suffice par excellence for studying the binding of Sos1 and Gab1 to Grb2 using biophysical methods."
sparser
"As shown in xref , it is clearly evident that the stoichiometries and energetics of binding of Sos1 and Gab1 peptides to Grb2 in Tris buffer versus Phosphate buffer are virtually indistinguishable."
sparser
"In light of these considerations, one would expect the binding of Sos1 and Gab1 peptides to full-length Grb2 with stoichiometries of 2:1 and 1:1, respectively."
sparser
"Although efforts by others and us directed at the determination of X-ray or NMR structures of full-length Grb2 bound to Sos1 or Gab1 constructs of any sort over the past decade or so have met no success, presumably due to the transient nature of Sos1-Grb2-Gab1 complex, we have nonetheless made an attempt here to create a crude 3D structural model of how the binding of one molecule of Sos1 to the nSH3 domain may allosterically induce a conformational change within Grb2 such that the loading of a second molecule of Sos1 onto the cSH3 domain is blocked and, in so doing, allowing Gab1 access to the cSH3 domain in an exclusively non-competitive manner to generate the Sos1-Grb2-Gab1 ternary complex ( xref )."
sparser
"Although these CD measurements are of highly qualitative nature and thus do not provide the physical basis of how the binding of Sos1 to the nSH3 domain may trigger a conformational change within Grb2 such that the nSH3 domain is only accessible to Gab1, the fact that Grb2 appears to be a highly flexible molecule by virtue of its ability to undergo discernable secondary and tertiary structural changes upon binding to Sos1 and Gab1 peptides nonetheless supports the notion that allostery is likely to play a central role in mediating communication between the nSH3 and cSH3 domains so as to allow the assembly of Sos1-Grb2-Gab1 ternary signaling complex in a non-competitive manner."
sparser
"On the contrary, our previous work has shown that the formation of Sos1-Grb2-Gab1 ternary complex is under tight allosteric control [ xref ]."
sparser
"Grb2 binds to both the Sos1 and Gab1 peptides with a 1:1 stoichiometry."
sparser
"Given that the formation of Sos1-Grb2-Gab1 ternary signaling complex proceeds in a non-competitive manner [ xref ], the most straightforward interpretation of these salient observations is that the Sos1 peptide binds only to the nSH3 domain but not the cSH3 domain, while the Gab1 peptide binds only to the cSH3 domain in the context of full-length Grb2."
sparser
"FRS2 was originally discovered as a docking site for coordinated assembly of a multiprotein complex that includes GRB2, GAB1, and SOS1, and serves a critical role in the FGFR signaling pathway (Sato and Gotoh xref )."
sparser
"Importantly, the formation of both the Grb2-Sos1 and Grb2-Gab1 binary complexes is governed by a stoichiometry of 2:1, whereby the respective SH3 domains within Grb2 homodimer bind to Sos1 and Gab1 via multivalent interactions."
SOS1 binds GRB2 and PGR. 7 / 7
| 7
sparser
"To understand stoichiometry and the underlying thermodynamic forces driving the formation of Grb2-Sos1 signaling complex, we next measured the binding of full-length Grb2 to the PR domain of Sos1 using ITC (representative data are shown in xref and all data summarized in xref )."
sparser
"Structural basis of the binding of Grb2 to the PR domain of Sos1 as two independent monomers versus a homodimer."
sparser
"Simply put, these observations strongly argue that the Grb2 homodimer bound to PR domain of Sos1 is structurally more stable than Grb2 monomers."
sparser
"MD simulations support the binding of Grb2 to the PR domain of Sos1 as a homodimer in lieu of two independent monomers."
sparser
"These salient observations indicate that the positive cooperativity associated with bivalent binding of Grb2 to the PR domain of Sos1 arises through both the formation of additional intermolecular contacts (favorable enthalpy) as well as burial of additional hydrophobic surfaces (favorable entropy)."
sparser
"In light of these observations, it is thus conceivable that Grb2 could bind to the PR domain of Sos1 either as two independent monomers (Grb2-PR-Grb2), or alternatively as a homodimer ([Grb2] 2 -PR)."
sparser
"Grb2 binds to the PR domain of Sos1 with a 2:1 stoichiometry."
SOS1 binds LAT and GRB2. 7 / 7
| 7
sparser
"Biophysical studies assessing the interactions between LAT, Grb2, and Sos1 have shown that Sos1 has two distinct Grb2-binding sites in its proline-rich region (PRR), and that Sos1 and Grb2 can form a 1:2 complex ( xref )."
sparser
"The exclusion of CD45 was recapitulated in a simpler system in which clusters were formed by pLAT, Grb2, and Sos1 ( xref )."
sparser
"Interestingly, activation of ERK1/2 is delayed relative to protein kinase C, xref suggesting that this alone is not sufficient to activate the MAPK pathway, leading us to speculate that this delay is as a result of assembly of the LAT-Grb2-SOS1 signaling complex."
sparser
"These data suggest an interesting hypothesis whereby multivalent, cooperative interactions between LAT, Grb2, and Sos1 promote LAT oligomerization, whereas recruitment of PLC-γ1–GADS-SLP-76 complexes block Grb2-LAT associations, and therefore limit the overall size of LAT oligomers."
sparser
"Furthermore, biophysical ( xref ) and modeling ( xref ) studies showed that multiple, cooperative interactions between LAT, Grb2, and Sos1 regulate the efficiency of higher-order LAT oligomerization."
sparser
"The existence of multivalent interactions between LAT:Grb2 (1:3) and Sos1:Grb2 (1:2) has led to the hypothesis that Grb2Sos1Grb2 complexes may nucleate LAT oligomerization ( xref )."
sparser
"Based on the multiplicity of Sos1:Grb2 and LAT:Grb2 interactions, it was hypothesized that the Grb2Sos1Grb2 complex could nucleate LAT oligomerization."
SOS1 binds CBL and GRB2. 5 / 5
| 5
sparser
"What the actual contacts are that drive the interaction of GRB2 with either SOS1 or CBL are outside of the scope of this study."
sparser
"Instead, as shown by our studies with the SOS1 scrambled mutant, regions outside of the consensus PxxPxR motifs drive the interaction of the complete proline rich region of SOS1 and CBL with full-length GRB2."
sparser
"In T cells, the SH2 domain of GRB2 binds phosphorylated tyrosines on the adaptor protein LAT and the GRB2 SH3 domains associate with the proline-rich regions of SOS1 and CBL."
sparser
"In contrast, these same PxxPxR motifs were neither necessary nor sufficient for the high affinity interaction of SOS1 or CBL with full-length GRB2 or required for the in vivo recruitment of SOS1 to the plasma membrane in activated T cells."
sparser
"To more fully address this prediction, we utilized quantitative biophysical and imaging techniques to examine the binding of GRB2 to its physiological ligands SOS1 and CBL."
SOS1 binds DOK3 and GRB2. 5 / 5
| 4 1
reach
"DOK3 contributes to the regulation of ERK activation downstream of LPS signaling and the formation of a Grb2, SOS1, and DOK3 complex appears to limit ERK activation."
sparser
"DOK3 was associated with Sos1 and Grb2 in unstimulated wild-type macrophages, but the extent of this association was considerably increased in response to low-dose LPS."
sparser
"Whereas our current studies showed that the association of DOK3 with Sos1 and Grb2 in response to low-dose LPS was required to inhibit ERK activation, at higher concentrations of TLR ligands, including LPS at >100 ng/ml and CpG at 0.5 µM, DOK3 becomes ubiquitinated and degraded ( xref )."
sparser
"These data suggest that the DAP12-dependent tyrosine phosphorylation of DOK3 is required for the association of DOK3 with Grb2 and Sos1."
sparser
"DOK3 contributes to the regulation of ERK activation downstream of LPS signaling and the formation of a Grb2-SOS1-DOK3 complex appears to limit ERK activation."
SOS1 binds EGFR and GRB2. 3 / 3
1 | 2
bel
"In response to stimulation with epidermal growth factor (EGF), the guanine nucleotide exchange factor human SOS1 (hSOS1) promotes the activation of Ras by forming a complex with Grb2 and the human EGF receptor (hEGFR)"
sparser
"Upon EGF stimulation, SOS1 binds to phosphorylated EGFR through the adaptor protein GRB2, which then triggers RAS activation at the plasma membrane ."
sparser
"Although the low-copy-number vector pBT(L) was the best in the case of EGFR-Grb2-Sos1 Y3H interaction, it may also cause reduced bait expression and lowered sensitivity for the detection of weaker protein interactions."
SOS1 binds GRB2 and PTK2. 3 / 3
3 |
bel
"FAK also combines with, and may activate, phosphoinositide 3-OH kinase (PI 3-kinase), either directly or through the Src kinase (13). Finally, there is evidence that Src phosphorylates FAK at Tyr925, creating a binding site for the complex of the adapter Grb2 and Ras guanosine 5'-triphosphate exchange factor mSOS (10). These interactions link FAK to signaling pathways that modify the cytoskeleton and activate mitogen-activated protein kinase (MAPK) cascades (Fig. 3A)."
bel
"FAK also combines with, and may activate, phosphoinositide 3-OH kinase (PI 3-kinase), either directly or through the Src kinase (13). Finally, there is evidence that Src phosphorylates FAK at Tyr925, creating a binding site for the complex of the adapter Grb2 and Ras guanosine 5'-triphosphate exchange factor mSOS (10). These interactions link FAK to signaling pathways that modify the cytoskeleton and activate mitogen-activated protein kinase (MAPK) cascades (Fig. 3A)."
bel
"In the first case, recruited FAK autophosphorylates at 397Y and associates with c-Src or Fyn, which further phosphorylates it at 925Y. Phosphorylated FAK binds to the Grb2-Sos complex and activates the Ras-ERK cascade [60,61]."
SHC binds SOS1 and GRB2. 3 / 3
| 2 1
sparser
"Together, these data suggest that the current model for regulation of p21ras, which proposes a stable association of Shc-grb2-Sos1 complexes at the plasma membrane, may be an oversimplification."
reach
"We also show that the Cbl and EGF receptor complex is predominantly associated with CrkII and is distinct to the Grb2, Shc, and Sos1 complex that associates with the EGF receptor."
sparser
"Ligation of the BCR with anti-IgM antibodies causes an elevation of Ras-GTP levels from about 15% to 25% and coincides with the formation of complexes containing phosphorylated Shc, Grb2 and Sos1 ."
SOS1 binds GRB2 and csh3. 3 / 3
| 3
sparser
"Both the N- and C-SH3 domains of grb2 interact with sos1 ( xref )."
sparser
"As shown in and , the binding of nSH3 and cSH3 domains of Grb2 to Sos1 employs distinct thermodynamic mechanisms."
sparser
"Such orientational constraint imposed upon Grb2-Sos1 assembly by the cSH3 domain coupled with conformational heterogeneity resulting from the promiscuity of nSH3 domain may be an important determinant of regulating the activation of downstream molecules such as Ras."
SOS1 binds GRB2 and GRB2. 3 / 3
| 3
reach
"This is based on the experimental dissociation constant,, for the formation of the Grb2, Sos1, and Grb2 complex from the Grb and Sos1 complex."
reach
"Based on the multiplicity of Sos1 : Grb2 and LAT : Grb2 interactions, it was hypothesized that the Grb2, Sos1, and Grb2 complex could nucleate LAT oligomerization."
reach
"This equation can be rewritten as : (9) or in other words : (10) where is the effective equilibrium constant for the binding of a Grb2 from solution to a Grb2 and Sos1 complex to form a Grb2, Sos1, and Grb2 complex : (11) To make predictions about the binding of Grb2 to the complete polyproline rich domain of Sos1 (1117-1319) we must estimate the values of the unknown equilibrium constants for the binding of the N- and C-SH3 domains of Grb2 to RP on Sos1."
SOS1 binds VAV1 and GRB2. 3 / 3
| 2 1
sparser
"LAT is tyrosine phosphorylated after TCR engagement and recruits molecular complexes formed by the adaptor protein Grb2 and the exchange factors Vav1 and Sos1 ( xref , xref )."
reach
"This adaptor does not contain ITAM domains in its cytoplasmic tail, but it contains one YINM motif, that, upon tyrosine phosphorylation recruits both PI3K and the Grb2, Vav1, and Sos1 complex."
sparser
"Grb2 binds constitutively to the proline rich regions of Sos1 and Vav1 through its N-terminal and C-terminal SH3 domains, respectively."
SHC1 binds SOS1 and GRB2. 2 / 2
1 1 |
hprd
No evidence text available
bel
"In the second case, Fyn or Yes, activated by some b1 and aV integrins, recruit Shc through their SH3 domain and phosphorylates it, thus promoting Shc association with the Grb2-Sos complex [62,63]."
RTK binds SOS1 and GRB2. 2 / 2
| 2
sparser
"The domain organization of SOS1 comprises of a histone fold domain, a Dbl homology (DH)-Pleckstrin homology (PH) domain, a Ras exchanger motif (REM), a CDC25-homology (CDC25-H) domain with GEF activity, and a proline-rich C-terminal region that binds Grb2 and links SOS1 to activated RTKs."
sparser
"Consequently, dn SH3 Grb2 prevents binding of the endogenous Grb2-SOS1 complex to RTKs."
SOS1 binds SOS2, GRB2, and RasGEF. 2 / 2
| 2
sparser
"Once phosphorylated, LAT normally associates with two regulators of Ras activation: PLC-γ1, which activates the RasGEF RasGRP1 via DAG, and Grb2, which directly associates with the RasGEFs Sos1 and Sos2 ( xref )."
sparser
"The RasGEFs Sos1 and Sos2 are constitutively associated with the adaptor Grb2 and are recruited to the membrane where they have basal RasGEF activity via Grb2-LAT interactions (Grb2 binds LAT pY175, pY195, and pY235) ( xref )."
SOS1 binds CBL, GRB2, and PRR. 2 / 2
| 2
sparser
"These observations fit well with previous studies showing that SOS1 peptides bind to individual SH3 domains from GRB2 with affinities ranging from 20μM to 1mM , while the affinity for the binding of f[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"These observations fit well with previous studies showing that SOS1 peptides bind to individual SH3 domains from GRB2 with affinities ranging from 20 µM to 1 mM [ xref – xref ], while the affinity for the binding of full-length GRB2 with full-length SOS1 or the complete PRR of SOS1 and CBL is between 300 and 400 nM ( xref ) and [ xref – xref ]."
SOS1 binds GRB2 and smo-1. 1 / 1
| 1
sparser
"The exact mechanism underlying Grb2 SUMOylation at K 56 recruiting more Sos1 to form SUMO-Grb2-Sos1 complex deserves further study."
SOS1 binds EGF and GRB2. 1 / 1
| 1
sparser
"Whilst the association of Sos1 and Grb2 following EGF stimulation is not cell type specific, we find that it is dependent on cell density and that the response to EGF differs to that induced by NGF."
SOS1 binds GRB2 and C-SH3 domain. 1 / 1
| 1
reach
"Thus, when the Grb2 concentration is much lower than the Sos1 concentration, we expect Grb2 to be doubly bound to Sos1 with its C-SH3 domain bound to the P1 domain."
SOS1 binds GRB2 and sem-5. 1 / 1
| 1
sparser
"Grb2-Sem-5 binds directly to murine Sos1, a Ras exchange factor, through two SH3 domains."
SOS1 binds CSF1R and GRB2. 1 / 1
| 1
sparser
"Fms also formed complexes with Grb2 and Sos1, and neither contained phosphotyrosine."
RRAS binds SOS1 and GRB2. 1 / 1
| 1
sparser
"This method has found wide application in demonstrating interactions between Bcl-2 and R-Ras p23 [45] , SOS1 and GRB2 [46] , Ras and Raf [47,48] , and Raf and 14-3-3 [49] ."
SOS1 binds GRB2 and PRR. 1 / 1
| 1
sparser
"These findings are in contrast to work by McDonald and coworkers, who showed that PxxPxR motifs were critical for the binding of GRB2 to the PRR or SOS1."
sparser
"We hence hypothesized that Cif increases Grb2-SOS1 binding to promote SOS1 activity."
SOS1 binds EPS8 and GRB2. 1 / 1
| 1
sparser
"In contrast, p66shcCH2 and -P47A/P50A led to little or no change in the amounts of grb2 and eps8 bound sos1 ( xref )."
SOS1 binds GRB2 and proline. 1 / 1
| 1
reach
"One LAT molecule is capable of binding up to three Grb2 molecules at a time andtwo Grb2 molecules bind one SOS1 or c-Cbl proline rich domain (XREF_FIG) 33."
SOS1 binds CAV1 and GRB2. 1 / 1
| 1
sparser
"In order to determine whether phospho-caveolin-1 expression sustains RAS signaling, we introduced in to HCT116-Cav-1 AS cells either a full-length caveolin-1 expression construct (WT) or a mutant form of caveolin-1 that is deficient in tyrosine phophorylation at residue 14 (Caveolin-1 Y14F) and assayed for the association of caveolin-1 with the two signaling molecules Grb2 and SOS1."
| 1
sparser
"There are also cases where orthologues in the invertebrates exist, but the interactions have not been observed experimentally as exemplified by the interaction of IIS and MAPK/ERK pathways via GRB2 and SOS1."
SOS1 binds FRS2 and GRB2. 1 / 1
| 1
sparser
"The hetero-oligomer formed between Sprouty1 and Sprouty4 exhibits the most potent inhibitory effect on ERK activation through its highly effective ability to suppress the association of Grb2-Sos1 complex with FRS2."
SOS1 binds GRB2 and E3b1. 1 / 1
| 1
sparser
"Thus, Sos-1 binds either E3b1 or Grb2, in a mutually exclusive fashion, suggesting that two distinct pools of Sos-1 exist in the cells."
SOS1 binds SOS2 and GRB2. 1 / 1
| 1
sparser
"The SH3 domain(s) of Grb2 bind to Sos1 and Sos2 (GEF of the Ras family of GTP-binding protein) ( xref ), SLP-76 ( xref , xref ), and Cbl ( xref , xref , xref )."
SOS1 binds GRB2 and Q07889. 1 / 1
| 1
reach
"A large number of these PPIs are mediated by multiple SLiM classes or SLiM instances, for example, in the interaction between GRB2 (uniprot : P62993) and SOS1 (uniprot : Q07889); SOS1 has seven putative SH3 motifs and GRB2 has two SH3 domains, potentially this can equate to 14 binding interfaces for a single PPI."
SOS1 binds DAB2 and GRB2. 1 / 1
| 1
sparser
"Since the two SH3 domains of Grb2 bind competitively to both Sos1 and Dab2, to their proline-rich motifs, expression levels and phosphorylation of Dab2 and Sos1 modulate the abundance of Sos1/Grb2 complexes."
RAS binds SOS1, GRB2, and PDGFRB. 1 / 1
| 1
sparser
"RasGAP binding to phospho-PDGFRβ causes simultaneous binding of the Grb2-SOS1 complex to Ras that offsets the activation of Ras and reduces the downstream activity of the extracellular signal-regulated protein kinases 1 and 2 (Erk1/2), as shown in vitro xref ."
SHC binds SOS1, EGFR, and GRB2. 1 / 1
| 1
sparser
"In response to calcium influx, Shc, Grb2, and Sos1 inducibly associate with a 180-kDa tyrosine-phosphorylated protein, which was determined to be the epidermal growth factor receptor (EGFR)."
SHC binds SOS1, CHKA, and GRB2. 1 / 1
| 1
sparser
"In our studies, we did not detect the increased association of Shc or Sos1 with Grb2 following expression of CHK (data not shown)."
SOS1 binds VAV1, GRB2, and PPFIA1. 1 / 1
| 1
sparser
"Finally, Kon-Kozlowski et al. ( xref ) have shown that the SH2-domain-containing protein tyrosine phosphatase 1 (SHP-1) associates with Vav1, Grb2, and Sos1."
SOS1 binds EPS8, GRB2, and E3b1. 1 / 1
| 1
sparser
"This model suggests a novel mechanism for Rac1 activation and predicts that the formation of the SNTA1-Grb2-P66shc complex determines the amount of cellular Sos1 that is bound to Grb2 or E3b1-Eps8; in this way, SNTA1 and P66shc modulate Rac1 activation and its downstream cellular functions."
sparser
"On the other hand, Grb2 and Sos1 are associated constitutively in U266 and RPMI8226 MM cells, but phosphorylation of Sos1 is not induced by IL-6."
SOS1 binds EPO, GAB1, and GRB2. 1 / 1
| 1
sparser
"The co-immunoprecipitation experiments revealed an EPO-enhanced association of Gab1 with the Grb2-SOS1 complex and SHP-2 in F-36P cells."
SOS1 binds SH3PXD2A, GRB2, and PRD. 1 / 1
| 1
sparser
"Consequently, Tks5 and Grb2 could simultaneously bind to the Sos1-PRD."
SOS1 binds SPRY1, SPRY4, and GRB2. 1 / 1
| 1
sparser
"They observed that while Spry1 and Spry4 interact with Grb2 and Sos1, respectively, the hetero-oligomer formed by the two exhibits the most potent inhibitory effect on FGF-activated ERK."
SOS1 binds WDR48, GRB2, and IL1R1. 1 / 1
| 1
sparser
"Sos1 and p80 proteins dominantly bind to Grb2 fusion proteins in Chang liver, whereas p100 remarkably associate with Grb2 in HepG2 cells."
SOS1 binds TJP1, CTTN, and GRB2. 1 / 1
| 1
sparser
"In addition, UCS15A was capable of disrupting other typical SH3-mediated protein-protein interactions such as Grb2-Sos1, cortactin-ZO1, as well as atypical SH3-mediated protein-protein interactions such as Grb2-Gab1."
| 1
sparser
"In the light of temporal and spatial control of the association of Tks5 isoforms and Grb2 with Sos1 and dynamin, it will be interesting to elucidate the dependence of complex formation on the phosphor[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
RTK binds SHC, SOS1, and GRB2. 1 / 1
| 1
sparser
"The IGF-I receptor is closely related to the insulin receptor although each has significantly different affinities for its cognate ligand. xref One branch of IGF-I’s mitogenic signaling involves the association of the receptor tyrosine kinase with Shc, Grb2, and Sos-1 to activate Ras and the MAP kinase cascades (Raf, Mek, Erk). xref One of the endpoints of the MAP kinase pathways is the modification of ELK transcription factors, serum response factor (SRF) and AP-1."
SOS1 binds GRB2, csh3, and nSH3. 1 / 1
| 1
sparser
"We have indeed previously shown that salt tightly modulates the binding of nSH3 and cSH3 domains of Grb2 to Sos1 ( xref )."
RTK binds SOS1, EGFR, and GRB2. 1 / 1
| 1
sparser
"In xref , experimental results show that GRB2 recruits SOS1 to the membrane to form GRB2-SOS1 complex upon the activation of EGFR or other RTKs."
ERK binds RAS, SOS1, and GRB2. 1 / 1
| 1
sparser
"Previous reports showed that pDOK3 associates with the cytosolic adaptor protein Grb2 (growth factor receptor–bound protein 2) and the Ras GEF Sos1 (son of sevenless 1) to prevent activation of the Ras-ERK pathway downstream of protein-tyrosine kinases in transfected human embryonic kidney (HEK) 293T cells ( xref )."
GAB1 affects GRB2, and SOS1
| 16
SOS1 binds GAB1 and GRB2. 10 / 16
| 16
sparser
"While we have relied here on short peptides to mimic Sos1 and Gab1, due largely to inherent difficulties associated with isolation and purification of full-length Sos1 and Gab1 proteins, it should be noted that these peptides suffice par excellence for studying the binding of Sos1 and Gab1 to Grb2 using biophysical methods."
sparser
"As shown in xref , it is clearly evident that the stoichiometries and energetics of binding of Sos1 and Gab1 peptides to Grb2 in Tris buffer versus Phosphate buffer are virtually indistinguishable."
sparser
"In light of these considerations, one would expect the binding of Sos1 and Gab1 peptides to full-length Grb2 with stoichiometries of 2:1 and 1:1, respectively."
sparser
"Although efforts by others and us directed at the determination of X-ray or NMR structures of full-length Grb2 bound to Sos1 or Gab1 constructs of any sort over the past decade or so have met no success, presumably due to the transient nature of Sos1-Grb2-Gab1 complex, we have nonetheless made an attempt here to create a crude 3D structural model of how the binding of one molecule of Sos1 to the nSH3 domain may allosterically induce a conformational change within Grb2 such that the loading of a second molecule of Sos1 onto the cSH3 domain is blocked and, in so doing, allowing Gab1 access to the cSH3 domain in an exclusively non-competitive manner to generate the Sos1-Grb2-Gab1 ternary complex ( xref )."
sparser
"Although these CD measurements are of highly qualitative nature and thus do not provide the physical basis of how the binding of Sos1 to the nSH3 domain may trigger a conformational change within Grb2 such that the nSH3 domain is only accessible to Gab1, the fact that Grb2 appears to be a highly flexible molecule by virtue of its ability to undergo discernable secondary and tertiary structural changes upon binding to Sos1 and Gab1 peptides nonetheless supports the notion that allostery is likely to play a central role in mediating communication between the nSH3 and cSH3 domains so as to allow the assembly of Sos1-Grb2-Gab1 ternary signaling complex in a non-competitive manner."
sparser
"On the contrary, our previous work has shown that the formation of Sos1-Grb2-Gab1 ternary complex is under tight allosteric control [ xref ]."
sparser
"Grb2 binds to both the Sos1 and Gab1 peptides with a 1:1 stoichiometry."
sparser
"Given that the formation of Sos1-Grb2-Gab1 ternary signaling complex proceeds in a non-competitive manner [ xref ], the most straightforward interpretation of these salient observations is that the Sos1 peptide binds only to the nSH3 domain but not the cSH3 domain, while the Gab1 peptide binds only to the cSH3 domain in the context of full-length Grb2."
sparser
"FRS2 was originally discovered as a docking site for coordinated assembly of a multiprotein complex that includes GRB2, GAB1, and SOS1, and serves a critical role in the FGFR signaling pathway (Sato and Gotoh xref )."
sparser
"Importantly, the formation of both the Grb2-Sos1 and Grb2-Gab1 binary complexes is governed by a stoichiometry of 2:1, whereby the respective SH3 domains within Grb2 homodimer bind to Sos1 and Gab1 via multivalent interactions."
GRB2 affects PGR, and SOS1
| 7
SOS1 binds GRB2 and PGR. 7 / 7
| 7
sparser
"To understand stoichiometry and the underlying thermodynamic forces driving the formation of Grb2-Sos1 signaling complex, we next measured the binding of full-length Grb2 to the PR domain of Sos1 using ITC (representative data are shown in xref and all data summarized in xref )."
sparser
"Structural basis of the binding of Grb2 to the PR domain of Sos1 as two independent monomers versus a homodimer."
sparser
"Simply put, these observations strongly argue that the Grb2 homodimer bound to PR domain of Sos1 is structurally more stable than Grb2 monomers."
sparser
"MD simulations support the binding of Grb2 to the PR domain of Sos1 as a homodimer in lieu of two independent monomers."
sparser
"These salient observations indicate that the positive cooperativity associated with bivalent binding of Grb2 to the PR domain of Sos1 arises through both the formation of additional intermolecular contacts (favorable enthalpy) as well as burial of additional hydrophobic surfaces (favorable entropy)."
sparser
"In light of these observations, it is thus conceivable that Grb2 could bind to the PR domain of Sos1 either as two independent monomers (Grb2-PR-Grb2), or alternatively as a homodimer ([Grb2] 2 -PR)."
sparser
"Grb2 binds to the PR domain of Sos1 with a 2:1 stoichiometry."
GRB2 affects LAT, and SOS1
| 7
SOS1 binds LAT and GRB2. 7 / 7
| 7
sparser
"Biophysical studies assessing the interactions between LAT, Grb2, and Sos1 have shown that Sos1 has two distinct Grb2-binding sites in its proline-rich region (PRR), and that Sos1 and Grb2 can form a 1:2 complex ( xref )."
sparser
"The exclusion of CD45 was recapitulated in a simpler system in which clusters were formed by pLAT, Grb2, and Sos1 ( xref )."
sparser
"Interestingly, activation of ERK1/2 is delayed relative to protein kinase C, xref suggesting that this alone is not sufficient to activate the MAPK pathway, leading us to speculate that this delay is as a result of assembly of the LAT-Grb2-SOS1 signaling complex."
sparser
"These data suggest an interesting hypothesis whereby multivalent, cooperative interactions between LAT, Grb2, and Sos1 promote LAT oligomerization, whereas recruitment of PLC-γ1–GADS-SLP-76 complexes block Grb2-LAT associations, and therefore limit the overall size of LAT oligomers."
sparser
"Furthermore, biophysical ( xref ) and modeling ( xref ) studies showed that multiple, cooperative interactions between LAT, Grb2, and Sos1 regulate the efficiency of higher-order LAT oligomerization."
sparser
"The existence of multivalent interactions between LAT:Grb2 (1:3) and Sos1:Grb2 (1:2) has led to the hypothesis that Grb2Sos1Grb2 complexes may nucleate LAT oligomerization ( xref )."
sparser
"Based on the multiplicity of Sos1:Grb2 and LAT:Grb2 interactions, it was hypothesized that the Grb2Sos1Grb2 complex could nucleate LAT oligomerization."
DOK3 affects GRB2, and SOS1
| 4 1
SOS1 binds DOK3 and GRB2. 5 / 5
| 4 1
reach
"DOK3 contributes to the regulation of ERK activation downstream of LPS signaling and the formation of a Grb2, SOS1, and DOK3 complex appears to limit ERK activation."
sparser
"DOK3 was associated with Sos1 and Grb2 in unstimulated wild-type macrophages, but the extent of this association was considerably increased in response to low-dose LPS."
sparser
"Whereas our current studies showed that the association of DOK3 with Sos1 and Grb2 in response to low-dose LPS was required to inhibit ERK activation, at higher concentrations of TLR ligands, including LPS at >100 ng/ml and CpG at 0.5 µM, DOK3 becomes ubiquitinated and degraded ( xref )."
sparser
"These data suggest that the DAP12-dependent tyrosine phosphorylation of DOK3 is required for the association of DOK3 with Grb2 and Sos1."
sparser
"DOK3 contributes to the regulation of ERK activation downstream of LPS signaling and the formation of a Grb2-SOS1-DOK3 complex appears to limit ERK activation."
CBL affects GRB2, and SOS1
| 5
SOS1 binds CBL and GRB2. 5 / 5
| 5
sparser
"What the actual contacts are that drive the interaction of GRB2 with either SOS1 or CBL are outside of the scope of this study."
sparser
"Instead, as shown by our studies with the SOS1 scrambled mutant, regions outside of the consensus PxxPxR motifs drive the interaction of the complete proline rich region of SOS1 and CBL with full-length GRB2."
sparser
"In T cells, the SH2 domain of GRB2 binds phosphorylated tyrosines on the adaptor protein LAT and the GRB2 SH3 domains associate with the proline-rich regions of SOS1 and CBL."
sparser
"In contrast, these same PxxPxR motifs were neither necessary nor sufficient for the high affinity interaction of SOS1 or CBL with full-length GRB2 or required for the in vivo recruitment of SOS1 to the plasma membrane in activated T cells."
sparser
"To more fully address this prediction, we utilized quantitative biophysical and imaging techniques to examine the binding of GRB2 to its physiological ligands SOS1 and CBL."
GRB2 affects SOS1, and csh3
| 3
SOS1 binds GRB2 and csh3. 3 / 3
| 3
sparser
"Both the N- and C-SH3 domains of grb2 interact with sos1 ( xref )."
sparser
"As shown in and , the binding of nSH3 and cSH3 domains of Grb2 to Sos1 employs distinct thermodynamic mechanisms."
sparser
"Such orientational constraint imposed upon Grb2-Sos1 assembly by the cSH3 domain coupled with conformational heterogeneity resulting from the promiscuity of nSH3 domain may be an important determinant of regulating the activation of downstream molecules such as Ras."
GRB2 affects SOS1, and VAV1
| 2 1
SOS1 binds VAV1 and GRB2. 3 / 3
| 2 1
sparser
"LAT is tyrosine phosphorylated after TCR engagement and recruits molecular complexes formed by the adaptor protein Grb2 and the exchange factors Vav1 and Sos1 ( xref , xref )."
reach
"This adaptor does not contain ITAM domains in its cytoplasmic tail, but it contains one YINM motif, that, upon tyrosine phosphorylation recruits both PI3K and the Grb2, Vav1, and Sos1 complex."
sparser
"Grb2 binds constitutively to the proline rich regions of Sos1 and Vav1 through its N-terminal and C-terminal SH3 domains, respectively."
GRB2 affects SHC, and SOS1
| 2 1
SHC binds SOS1 and GRB2. 3 / 3
| 2 1
sparser
"Together, these data suggest that the current model for regulation of p21ras, which proposes a stable association of Shc-grb2-Sos1 complexes at the plasma membrane, may be an oversimplification."
reach
"We also show that the Cbl and EGF receptor complex is predominantly associated with CrkII and is distinct to the Grb2, Shc, and Sos1 complex that associates with the EGF receptor."
sparser
"Ligation of the BCR with anti-IgM antibodies causes an elevation of Ras-GTP levels from about 15% to 25% and coincides with the formation of complexes containing phosphorylated Shc, Grb2 and Sos1 ."
GRB2 affects PTK2, and SOS1
3 |
SOS1 binds GRB2 and PTK2. 3 / 3
3 |
bel
"FAK also combines with, and may activate, phosphoinositide 3-OH kinase (PI 3-kinase), either directly or through the Src kinase (13). Finally, there is evidence that Src phosphorylates FAK at Tyr925, creating a binding site for the complex of the adapter Grb2 and Ras guanosine 5'-triphosphate exchange factor mSOS (10). These interactions link FAK to signaling pathways that modify the cytoskeleton and activate mitogen-activated protein kinase (MAPK) cascades (Fig. 3A)."
bel
"FAK also combines with, and may activate, phosphoinositide 3-OH kinase (PI 3-kinase), either directly or through the Src kinase (13). Finally, there is evidence that Src phosphorylates FAK at Tyr925, creating a binding site for the complex of the adapter Grb2 and Ras guanosine 5'-triphosphate exchange factor mSOS (10). These interactions link FAK to signaling pathways that modify the cytoskeleton and activate mitogen-activated protein kinase (MAPK) cascades (Fig. 3A)."
bel
"In the first case, recruited FAK autophosphorylates at 397Y and associates with c-Src or Fyn, which further phosphorylates it at 925Y. Phosphorylated FAK binds to the Grb2-Sos complex and activates the Ras-ERK cascade [60,61]."
EGFR affects GRB2, and SOS1
1 | 2
SOS1 binds EGFR and GRB2. 3 / 3
1 | 2
bel
"In response to stimulation with epidermal growth factor (EGF), the guanine nucleotide exchange factor human SOS1 (hSOS1) promotes the activation of Ras by forming a complex with Grb2 and the human EGF receptor (hEGFR)"
sparser
"Upon EGF stimulation, SOS1 binds to phosphorylated EGFR through the adaptor protein GRB2, which then triggers RAS activation at the plasma membrane ."
sparser
"Although the low-copy-number vector pBT(L) was the best in the case of EGFR-Grb2-Sos1 Y3H interaction, it may also cause reduced bait expression and lowered sensitivity for the detection of weaker protein interactions."
GRB2 affects SHC1, and SOS1
1 1 |
SHC1 binds SOS1 and GRB2. 2 / 2
1 1 |
hprd
No evidence text available
bel
"In the second case, Fyn or Yes, activated by some b1 and aV integrins, recruit Shc through their SH3 domain and phosphorylates it, thus promoting Shc association with the Grb2-Sos complex [62,63]."
GRB2 affects RasGEF, SOS1, and SOS2
| 2
SOS1 binds SOS2, GRB2, and RasGEF. 2 / 2
| 2
sparser
"Once phosphorylated, LAT normally associates with two regulators of Ras activation: PLC-γ1, which activates the RasGEF RasGRP1 via DAG, and Grb2, which directly associates with the RasGEFs Sos1 and Sos2 ( xref )."
sparser
"The RasGEFs Sos1 and Sos2 are constitutively associated with the adaptor Grb2 and are recruited to the membrane where they have basal RasGEF activity via Grb2-LAT interactions (Grb2 binds LAT pY175, pY195, and pY235) ( xref )."
GRB2 affects RTK, and SOS1
| 2
RTK binds SOS1 and GRB2. 2 / 2
| 2
sparser
"The domain organization of SOS1 comprises of a histone fold domain, a Dbl homology (DH)-Pleckstrin homology (PH) domain, a Ras exchanger motif (REM), a CDC25-homology (CDC25-H) domain with GEF activity, and a proline-rich C-terminal region that binds Grb2 and links SOS1 to activated RTKs."
sparser
"Consequently, dn SH3 Grb2 prevents binding of the endogenous Grb2-SOS1 complex to RTKs."
CBL affects GRB2, PRR, and SOS1
| 2
SOS1 binds CBL, GRB2, and PRR. 2 / 2
| 2
sparser
"These observations fit well with previous studies showing that SOS1 peptides bind to individual SH3 domains from GRB2 with affinities ranging from 20μM to 1mM , while the affinity for the binding of f[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"These observations fit well with previous studies showing that SOS1 peptides bind to individual SH3 domains from GRB2 with affinities ranging from 20 µM to 1 mM [ xref – xref ], while the affinity for the binding of full-length GRB2 with full-length SOS1 or the complete PRR of SOS1 and CBL is between 300 and 400 nM ( xref ) and [ xref – xref ]."
GRB2 affects SOS1, and smo-1
| 1
SOS1 binds GRB2 and smo-1. 1 / 1
| 1
sparser
"The exact mechanism underlying Grb2 SUMOylation at K 56 recruiting more Sos1 to form SUMO-Grb2-Sos1 complex deserves further study."
GRB2 affects SOS1, and sem-5
| 1
SOS1 binds GRB2 and sem-5. 1 / 1
| 1
sparser
"Grb2-Sem-5 binds directly to murine Sos1, a Ras exchange factor, through two SH3 domains."
GRB2 affects SOS1, and proline
| 1
SOS1 binds GRB2 and proline. 1 / 1
| 1
reach
"One LAT molecule is capable of binding up to three Grb2 molecules at a time andtwo Grb2 molecules bind one SOS1 or c-Cbl proline rich domain (XREF_FIG) 33."
GRB2 affects SOS1, and cycle inhibiting factor (Cif)
| 1
sparser
"We hence hypothesized that Cif increases Grb2-SOS1 binding to promote SOS1 activity."
GRB2 affects SOS1, csh3, and nSH3
| 1
SOS1 binds GRB2, csh3, and nSH3. 1 / 1
| 1
sparser
"We have indeed previously shown that salt tightly modulates the binding of nSH3 and cSH3 domains of Grb2 to Sos1 ( xref )."
GRB2 affects SOS1, SPRY1, and SPRY4
| 1
SOS1 binds SPRY1, SPRY4, and GRB2. 1 / 1
| 1
sparser
"They observed that while Spry1 and Spry4 interact with Grb2 and Sos1, respectively, the hetero-oligomer formed by the two exhibits the most potent inhibitory effect on FGF-activated ERK."
GRB2 affects SOS1, and SOS2
| 1
SOS1 binds SOS2 and GRB2. 1 / 1
| 1
sparser
"The SH3 domain(s) of Grb2 bind to Sos1 and Sos2 (GEF of the Ras family of GTP-binding protein) ( xref ), SLP-76 ( xref , xref ), and Cbl ( xref , xref , xref )."
GRB2 affects SCGB1D4, and SOS1
| 1
| 1
sparser
"There are also cases where orthologues in the invertebrates exist, but the interactions have not been observed experimentally as exemplified by the interaction of IIS and MAPK/ERK pathways via GRB2 and SOS1."
GRB2 affects RTK, SHC, and SOS1
| 1
RTK binds SHC, SOS1, and GRB2. 1 / 1
| 1
sparser
"The IGF-I receptor is closely related to the insulin receptor although each has significantly different affinities for its cognate ligand. xref One branch of IGF-I’s mitogenic signaling involves the association of the receptor tyrosine kinase with Shc, Grb2, and Sos-1 to activate Ras and the MAP kinase cascades (Raf, Mek, Erk). xref One of the endpoints of the MAP kinase pathways is the modification of ELK transcription factors, serum response factor (SRF) and AP-1."
GRB2 affects RRAS, and SOS1
| 1
RRAS binds SOS1 and GRB2. 1 / 1
| 1
sparser
"This method has found wide application in demonstrating interactions between Bcl-2 and R-Ras p23 [45] , SOS1 and GRB2 [46] , Ras and Raf [47,48] , and Raf and 14-3-3 [49] ."
GRB2 affects Q07889, and SOS1
| 1
SOS1 binds GRB2 and Q07889. 1 / 1
| 1
reach
"A large number of these PPIs are mediated by multiple SLiM classes or SLiM instances, for example, in the interaction between GRB2 (uniprot : P62993) and SOS1 (uniprot : Q07889); SOS1 has seven putative SH3 motifs and GRB2 has two SH3 domains, potentially this can equate to 14 binding interfaces for a single PPI."
GRB2 affects PRR, and SOS1
| 1
SOS1 binds GRB2 and PRR. 1 / 1
| 1
sparser
"These findings are in contrast to work by McDonald and coworkers, who showed that PxxPxR motifs were critical for the binding of GRB2 to the PRR or SOS1."
GRB2 affects PRD, SH3PXD2A, and SOS1
| 1
SOS1 binds SH3PXD2A, GRB2, and PRD. 1 / 1
| 1
sparser
"Consequently, Tks5 and Grb2 could simultaneously bind to the Sos1-PRD."
GRB2 affects PPFIA1, SOS1, and VAV1
| 1
SOS1 binds VAV1, GRB2, and PPFIA1. 1 / 1
| 1
sparser
"Finally, Kon-Kozlowski et al. ( xref ) have shown that the SH2-domain-containing protein tyrosine phosphatase 1 (SHP-1) associates with Vav1, Grb2, and Sos1."
GRB2 affects PDGFRB, RAS, and SOS1
| 1
RAS binds SOS1, GRB2, and PDGFRB. 1 / 1
| 1
sparser
"RasGAP binding to phospho-PDGFRβ causes simultaneous binding of the Grb2-SOS1 complex to Ras that offsets the activation of Ras and reduces the downstream activity of the extracellular signal-regulated protein kinases 1 and 2 (Erk1/2), as shown in vitro xref ."
GRB2 affects IL1R1, SOS1, and WDR48
| 1
SOS1 binds WDR48, GRB2, and IL1R1. 1 / 1
| 1
sparser
"Sos1 and p80 proteins dominantly bind to Grb2 fusion proteins in Chang liver, whereas p100 remarkably associate with Grb2 in HepG2 cells."
FRS2 affects GRB2, and SOS1
| 1
SOS1 binds FRS2 and GRB2. 1 / 1
| 1
sparser
"The hetero-oligomer formed between Sprouty1 and Sprouty4 exhibits the most potent inhibitory effect on ERK activation through its highly effective ability to suppress the association of Grb2-Sos1 complex with FRS2."
FRS2 affects GAB1, GRB2, PTPN11, SH2B1, SHC1, and SOS1
| 1
| 1
sparser
"Stimulation of different cell types with FGF-1 resulted in the formation of multiple complexes involving FRS2, GAB1, SOS1, PTPN11, SHC1, SH2B1, and GRB2 [ xref – xref ]."
ERK affects GRB2, RAS, and SOS1
| 1
ERK binds RAS, SOS1, and GRB2. 1 / 1
| 1
sparser
"Previous reports showed that pDOK3 associates with the cytosolic adaptor protein Grb2 (growth factor receptor–bound protein 2) and the Ras GEF Sos1 (son of sevenless 1) to prevent activation of the Ras-ERK pathway downstream of protein-tyrosine kinases in transfected human embryonic kidney (HEK) 293T cells ( xref )."
EPS8 affects GRB2, and SOS1
| 1
SOS1 binds EPS8 and GRB2. 1 / 1
| 1
sparser
"In contrast, p66shcCH2 and -P47A/P50A led to little or no change in the amounts of grb2 and eps8 bound sos1 ( xref )."
EPO affects GAB1, GRB2, and SOS1
| 1
SOS1 binds EPO, GAB1, and GRB2. 1 / 1
| 1
sparser
"The co-immunoprecipitation experiments revealed an EPO-enhanced association of Gab1 with the Grb2-SOS1 complex and SHP-2 in F-36P cells."
EGFR affects GRB2, SHC, and SOS1
| 1
SHC binds SOS1, EGFR, and GRB2. 1 / 1
| 1
sparser
"In response to calcium influx, Shc, Grb2, and Sos1 inducibly associate with a 180-kDa tyrosine-phosphorylated protein, which was determined to be the epidermal growth factor receptor (EGFR)."
EGFR affects GRB2, RTK, and SOS1
| 1
RTK binds SOS1, EGFR, and GRB2. 1 / 1
| 1
sparser
"In xref , experimental results show that GRB2 recruits SOS1 to the membrane to form GRB2-SOS1 complex upon the activation of EGFR or other RTKs."
EGF affects GRB2, and SOS1
| 1
SOS1 binds EGF and GRB2. 1 / 1
| 1
sparser
"Whilst the association of Sos1 and Grb2 following EGF stimulation is not cell type specific, we find that it is dependent on cell density and that the response to EGF differs to that induced by NGF."
E3b1 affects GRB2, and SOS1
| 1
SOS1 binds GRB2 and E3b1. 1 / 1
| 1
sparser
"Thus, Sos-1 binds either E3b1 or Grb2, in a mutually exclusive fashion, suggesting that two distinct pools of Sos-1 exist in the cells."
E3b1 affects EPS8, GRB2, and SOS1
| 1
SOS1 binds EPS8, GRB2, and E3b1. 1 / 1
| 1
sparser
"This model suggests a novel mechanism for Rac1 activation and predicts that the formation of the SNTA1-Grb2-P66shc complex determines the amount of cellular Sos1 that is bound to Grb2 or E3b1-Eps8; in this way, SNTA1 and P66shc modulate Rac1 activation and its downstream cellular functions."
DNM affects GRB2, SH3PXD2A, and SOS1
| 1
| 1
sparser
"In the light of temporal and spatial control of the association of Tks5 isoforms and Grb2 with Sos1 and dynamin, it will be interesting to elucidate the dependence of complex formation on the phosphor[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
DAB2 affects GRB2, and SOS1
| 1
SOS1 binds DAB2 and GRB2. 1 / 1
| 1
sparser
"Since the two SH3 domains of Grb2 bind competitively to both Sos1 and Dab2, to their proline-rich motifs, expression levels and phosphorylation of Dab2 and Sos1 modulate the abundance of Sos1/Grb2 complexes."
CTTN affects GRB2, SOS1, and TJP1
| 1
SOS1 binds TJP1, CTTN, and GRB2. 1 / 1
| 1
sparser
"In addition, UCS15A was capable of disrupting other typical SH3-mediated protein-protein interactions such as Grb2-Sos1, cortactin-ZO1, as well as atypical SH3-mediated protein-protein interactions such as Grb2-Gab1."
CSF1R affects GRB2, and SOS1
| 1
SOS1 binds CSF1R and GRB2. 1 / 1
| 1
sparser
"Fms also formed complexes with Grb2 and Sos1, and neither contained phosphotyrosine."
CSAG2 affects GRB2, Multiple Myeloma, and SOS1
| 1
sparser
"On the other hand, Grb2 and Sos1 are associated constitutively in U266 and RPMI8226 MM cells, but phosphorylation of Sos1 is not induced by IL-6."
CHKA affects GRB2, SHC, and SOS1
| 1
SHC binds SOS1, CHKA, and GRB2. 1 / 1
| 1
sparser
"In our studies, we did not detect the increased association of Shc or Sos1 with Grb2 following expression of CHK (data not shown)."
CBL affects CD3, FUT4, GRB2, LAT2, and SOS1
| 1
CD3 binds SOS1, LAT2, CBL, FUT4, and GRB2. 1 / 1
| 1
sparser
"After stimulation of the transfectants with an agonistic CD3 mAb, NTAL became rapidly tyrosine-phosphorylated and associated with Grb2, Sos1, and c-Cbl ( xref A) but not with SLP-76 or PLCγ1 (unpublished data)."
CAV1 affects GRB2, and SOS1
| 1
SOS1 binds CAV1 and GRB2. 1 / 1
| 1
sparser
"In order to determine whether phospho-caveolin-1 expression sustains RAS signaling, we introduced in to HCT116-Cav-1 AS cells either a full-length caveolin-1 expression construct (WT) or a mutant form of caveolin-1 that is deficient in tyrosine phophorylation at residue 14 (Caveolin-1 Y14F) and assayed for the association of caveolin-1 with the two signaling molecules Grb2 and SOS1."
C-SH3 domain affects GRB2, and SOS1
| 1
SOS1 binds GRB2 and C-SH3 domain. 1 / 1
| 1
reach
"Thus, when the Grb2 concentration is much lower than the Sos1 concentration, we expect Grb2 to be doubly bound to Sos1 with its C-SH3 domain bound to the P1 domain."
BCAR1 affects BLNK, CRK, GRB2, PIK3R1, SH3KBP1, and SOS1
1 |
1 |
hprd
No evidence text available