"To further clarify the interaction between specific transcription factors, such as C/EBPβ, YY1 and Smad3, in the mouse Amtn gene promoter in vivo , chromatin immunoprecipitation assays (ChIP) assays were carried out."
"Hence, we proposed that Pg LPS increased Smad3 binding to C/EBPβ and YY1, and thereby upregulated Amtn gene transcription."
"Considering to the results of C/EBPβ, YY1, Smad3 and pSmad3 protein levels after stimulation by Pg LPS (Fig. xref and Table 3), LPS‐induced C/EBPβ, YY1 and pSmad3 directly bind to C/EBP1, 2 and YY1 elements, respectively, mediating their physical interaction with Smad3."
"Furthermore, we identifiedLPS‐modulated interactions with C/EBPβ,YY1 and Smad3."
"Here, we report that Pg LPS‐induced physical interactions between C/EBPβ, YY1 and Smad3 upregulate Amtn gene transcription via mitogen‐activated protein kinase kinase 1/2 (MEK1/2), phosphatidylinositol 3‐kinase (PI3‐K) and Smad2/3 pathways in mouse gingival epithelial cells."
"C/EBPβ and YY1 directly interact with Smad3 in GE1 cells."
"In this study we have shown that Pg LPS induced Amtn gene transcription that was mediated through C/EBPβ, YY1 and Smad3 binding to C/EBP1, C/EBP2 and YY1 elements, which was accompanied by physical interaction between C/EBPβ, YY1 and Smad3."