IndraLab

"The use of digital technology in dentistry has increased in recent years [ xref ], thanks to different computer-aided design and computer-aided manufacturing (CAD-CAM) systems being used to fabricate different types of prostheses [ xref ] and to intraoral scanners (IOSs) that allow us to obtain a full digital workflow from the impression to the delivery."

"The type of CAD-CAM manufacturing method-additive or subtractive-significantly influenced the trueness and internal fit of resin-based definitive crowns."

"The purpose of this in vitro study was to evaluate the denture base adaptation of computer-aided design and computer-aided manufactured (CAD-CAM) milled, 3D printed, and conventional heat-polymerized resin fabrication techniques."

"Maxillary and mandibular edentulous models fabricated from cobalt-chromium alloy were scanned, and 2-mm-thick denture bases were designed and fabricated by using 4 fabrication techniques and materials: CAD-CAM milled (CCM), 3D printed (3DP), injection molded (IM), and compression molded (CM)."

"Till now, the literature has not defined the minimum clinically acceptable marginal fit of CAD-CAM zirconia restoration."

"Zirconia frameworks milled by computer-aided design and computer-aided manufacturing (CAD-CAM) often require clinical adjustments."

"Time-consuming manual fabrication stages are eliminated thanks to CAD-CAM technology."

"Tested stains on CAD-CAM ceramic had varying color changes after aging."

"When the two CAD-CAM materials were compared, RNC showed superior flexural strength than PICN, regardless of the type of resin cement used."

"This study aimed to evaluate how the transmucosal contour of posterior single-tooth implant crowns, fabricated with CAD-CAM abutments, influences peri-implant soft tissue dimensions using the profile angle and crown ratio concepts."

"HET-CAM."

"While both formulations showed good biocompatibility in the hen’s egg test on chorioallantoic membrane (HET-CAM), they substantially affected the three-dimensional U87 xenografts on the CAM, emphasized by PET/CT imaging and histological analysis. xref , xref "

"The 3Rs principle based on replacement, reduction, and refinement was derived from this directive and set the focus on the use of alternative methods. xref As early as 1985, HET-CAM contributed to the 3Rs principle and is now acknowledged as an alternative test option to the Draize test. xref It can be used to investigate the potential for irritation and the biocompatibility of a formulation."

"On EDD 9, HET-CAM was performed by applying 300 μL of each formulation (5 mg/mL) and monitoring the CAM over 5 min."

"The applied concentrations of PS in the HET-CAM were much higher than the phototoxic ones in vitro (see xref )."

"As expected from the HET-CAM results, visible responses could not be identified directly after application."

"The HET-CAM assay, which stands for the Hen’s Egg Test on Chorioallantoic Membrane, is a valuable assay for evaluating the effects of hydrogel formulations."

"The HET-CAM assay is also known for its speed and simplicity, making it a feasible alternative for evaluating potential irritant properties of formulations [ xref ]."

"HCEC test confers corneal biocompatibility while the HET-CAM relates to conjunctival (and vascular in general) effects."

"Further, it was observed that the complexation aided in the improved ex vivo retention and permeation, while the complexes were biocompatible as confirmed by the MTT and HET-CAM assay."

"Beyond acting as a physical barrier, the ECM also contributes to drug resistance through CAM-DR [ xref , xref , xref ]."

"These data suggest that MM cells share common regulatory pathways for drug resistance in different models, including SFM-DR and CAM-DR."

"The phenomenon of CAM-DR is particularly relevant in the context of drug resistance."

"It is accepted that CAM-DR contributes drug resistance to melphalan, vincristine, doxorubicin, mitoxantrone, and bortezomib [ xref , xref ]."

"MM cells also induce CAM-DR by binding BMSCs directly."

"Notably, the COL1 induced CAM-DR adds as an independent process to the pre-existing cisplatin resistance in W1CR cells, which is a novel and unexpected finding improving our understanding."

"However, some questions related to both CAM-RR and CAM-DR remain largely untouched."

"The interaction of tumor cells with their microenvironment allows tumor cells to be sheltered in the microenvironment and mediate drug resistance, called cell adhesion-mediated drug resistance (CAM-DR[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Currently, the most common model of CAM-DR is the spherical model, Fibronectin (Fn) model and matrix model [ 4 ]."

"In this study, we established the CAM-DR model of glioma cells using human Fn in vitro , and investigated the underlying mechanisms of TMZ insensitivity."

"In summary, Numbl plays a significant role in the formation of CAM-DR in MM cells."

"Observations in one study indicated that CAM-DR in MM cells was associated with increase in solubility and redistribution of c-FLIP L allowing its binding to and inhibiting death-inducing signaling complex (DISC) (which forms following CD95 ligation) and thus blocking apoptosis [ xref ]."

"For example, anticancer drug-induced H3K27 hypermethylation is associated with CAM-DR in MM cells [ xref ]."

"Finally, we showed that the interaction of Numbl with integrin β1 promotes the formation of CAM-DR in MM cells."

"We validated our pipeline using simulated and experimental data from the Cambridge Centre for Aging and Neuroscience (Cam-CAN) dataset."

"To test our claim, we evaluate the degree of neural coordination using phase synchrony measures xref at different regimes of alpha frequency band in resting-state MEG recordings collected from participants aged 18–88 at the Cambridge Center for Ageing and Neuroscience (Cam-CAN) xref ."

"MEG recordings from the Cam-CAN lifespan cohort (age range 18–88 years) were used to characterize the relationship of network frequency and phase coupling across age."

"We validate this hypothesis by employing two complementary measures of phase synchrony—Phase Locking Value (PLV) and Phase Lag Index (PLI) on source-reconstructed MEG data made publicly available by Cam-CAN."

"The spatial distribution of the CRON in the HCP and Cam-CAN samples is shown in xref A, B and C. As in the PRONIA study (Supplementary Fig. 2), the neurosignature comprised lower gray matter volume in cingulate, orbitofrontal, insular, temporal, parietal, and occipital brain regions, and higher cerebellar and prefrontal volumes."

"The proportion of CRON- Pos and CRON- Neg individuals is shown in xref C for the Cam-CAN sample and Supplementary Fig. 4 for the HCP sample."

"The distribution of decision scores in the Cam-CAN and HCP samples is shown in xref E. Even at uncorrected P-values, there was no association between decision scores and whole-brain gray matter volume in the young (r = 0.02, P = 0.82) and older Cam-CAN sub-sets (Pearson's r = 0.06, P = 0.28) and in the HCP sample (Pearson's r = −0.03, P = 0.27)."

"Specifically, although the CRON could detected in a subset of both the HCP- and Cam-CAN samples, its presence was not associated with dysfunction on any measure of social functioning."

"To get around the high cost of clinical trials, future studies could consider using large, publicly available databases such as the UK Biobank ( Cox et al., 2016 ) and Cam-CAN ( Taylor et al., 2017a )[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"To test this hypothesis, the CHR-outcome-neurosignature derived from the PRONIA study, was used to build a binary classifier which was applied to sMRI data of participants from the Human Connectome Project (HCP) ( xref ) and the Cambridge Centre for Ageing and Neuroscience project (Cam-CAN) ( xref )."

"Similarly, knockdown of TGM2 mirrored the effects of CTCF KO, impairing the proliferative capacity of CALM‐AF10 AML cells and promoting macrophage‐like differentiation."

"However, TGM2 overexpression could not rescue the CTCF knockout phenotype (Figure  xref ), suggesting that TGM2 functions as only one component of CTCF's broader regulatory network governing CALM‐AF10 AML differentiation."

"These findings strongly support the existence of a CTCF–TGM2 regulatory axis critical for maintaining the undifferentiated state of CALM‐AF10 AML cells."

"GK921 [ xref , xref , xref , xref , xref ] (Selleck Biotech, Kanagawa, Japan), a TGM2 inhibitor, was investigated in CALM‐AF10 AML cells."

"Within this subgroup of immature T-ALL, CALM-AF10 fusions are mostly seen in the AYA population with a median age of 25 years old, and are associated with an early resistance to treatment and a poor o[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Oncogenic transcription factors can be divided into several groups: TAL1/2 , LMO1 /2, TLX1 /3, LYL , HOXA- translocations, MEF2C , NKX1/2 , CALM::AF10 , and KMT2A -rearrangements ( KMT2A -r)."

"May Grunwald Giemsa staining and FACS analysis confirmed that GK921 induced differentiation of CALM‐AF10 AML cells into macrophage‐like cells, as evidenced by increased expression of F4/80 (Figure  xref )."

"MN1 (Meningioma-1) is frequently over-expressed in AML patients and is associated with a poor prognosis. xref – xref However, in patients with inv(16), highest MN1 expression has been reported with favorable prognosis to current therapeutics. xref MN1 functions as a transcriptional regulator that co-operates with the nuclear receptors for retinoic acid (RAR) and vitamin D, by acting as co-activator or co-repressor, depending on the interacting partners. xref – xref In addition, MN1 is frequently over-expressed and occasionally fused to TEL as part of the rare MN1-TEL translocation. xref Mn1 is known to be co-operating partner of several oncogenic fusion genes (NUP98–HOXD13, xref CALM–AF10, xref MLL–AF9 xref and MLL–ENL) xref and mutated RUNX1, xref and as a common target of insertional mutagenesis in a hematopoietic stem cell (HSC) gene therapy trial, xref thereby promoting leukemogenesis."

"These results indicate that GK921 effectively targets the undifferentiated state and proliferation of CALM‐AF10 AML cells."

"Furthermore, ChIP‐qPCR analysis revealed that CALM‐AF10 binds to the HOXA locus but not to the TGM2 locus, suggesting that TGM2 is unlikely to be a direct transcriptional target of CALM‐AF10 (Figure  xref )."

"The observation that CRM1 binds Hoxa chromatin in CALM-AF10 expressing MEFs led us to question whether CRM1 is also present at HOXA loci in leukemia cells."

"We have demonstrated that in addition to mediating nuclear export of CALM-AF10, the interaction of CRM1 with CALM-AF10 directly regulates HOXA cluster gene expression."

"We have demonstrated that a CRM1-AF10 fusion protein phenocopies CALM-AF10 in its ability to bind and activate Hoxa and Meis1 genes and to induce leukemia, supporting a model in which CRM1 enables the tethering of CALM-AF10 to Hoxa and Meis1 effector genes, thereby recruiting the AF10/DOT1L transcriptional complex and causing leukemia."

"CALM-AF10 interacts with Crm1 via a Nuclear Export Signal (NES) contained in the CALM moiety, and mutation of the NES or pharmacological inhibition of the Crm1/NES interaction abolishes the ability of CALM-AF10 to bind to and activate the transcription of Hoxa genes."

"Interaction of CALM-AF10 with CRM1 is necessary to upregulate Hoxa gene expression."

"Together, these results suggest that the interaction of CALM-AF10 with CRM1 is necessary for upregulation of Hoxa cluster gene expression."

"Our results thus far support a model in which CALM-AF10 must interact with CRM1 (via the NES) to upregulate Hoxa gene transcription."

"Our observation that CALM-AF10 interacts with Hoxa chromatin in a CRM1-dependent manner led us to hypothesize that CRM1 itself may bind Hoxa loci thus allowing for recruitment of CALM-AF10."

"The agreement between the family member’s detection of delirium in the case vignette was moderate using the FAM-CAM (kappa = 0.44) and substantial using the Sour Seven (kappa = 0.75)."

"This included 78% and 92% of participants correctly classifying delirium in the provided case vignettes using the FAM-CAM and Sour Seven, respectively."

"In addition, the readability of the FAM-CAM (Flesch-Kinkaid reading level ~11 th grade) and the Sour Seven (Flesch-Kinkaid reading level ~12 th grade) are higher than the suggested grade six level."

"Efforts to improve the readability of the FAM-CAM and Sour Seven may improve family member’s understanding of the questionnaires and may improve their identification of disorganized thinking and altered level of consciousness."

"In this issue of CCM, Fiest et at [ref] present results from a diagnostic accuracy study in which they test two family administered delirium screening tools, the Family Confusion Assessment Method (FAM-CAM) ( xref ), and the Sour Seven ( xref ) against the diagnosis of delirium according to the Diagnosis and Statistical Manual of Mental Disorders, Edition 5 (DSM-5)."

"In previous studies, some of the trained interviewers using the CAM were not blinded to the FAM-CAM results and used the results of the FAM-CAM to score specific CAM items, such as acute change."

"These approaches likely increased agreement between the FAM-CAM and CAM ratings. xref "

"However, in at risk patients in the ED where delirium status was unclear it would be feasible for a nurse or physician to provide a copy of the FAM-CAM to caregivers to complete."

"Results from the FAM-CAM could then be used by clinicians to inform their own assessments for delirium and subsequent medical decision making."

"An additional benefit of the FAM-CAM is that it may empower caregivers to assess for delirium in family members outside the hospital and seeking medical care in a timely fashion."

"With CAM-B3LYP a further fine-tuning of the calculated electronic energy is provided."

"Time-dependent DFT calculations were carried out in both the singlet and triplet state at CAM-B3LYP xref /LANL2DZ level of theory using the default continuum model (PCM) to introduce the solvent effect."

"Yet, the relative stability of the isomers containing a given number of HS centres differs according to the relation CAM-B3LYP ≥ B3LYP > B3LYP*."

"The plots of Δ G ( T ) calculated with B3LYP and CAM-B3LYP of all spin isomers of 1, 2, 2a and 3 are shown in xref and xref ."

"For this reason ultimately experimental and CAM-B3LYP results are compared with each other."

"It was shown that all excitation wavelengths were lowered when CAM-B3LYP was used (in contrast to B3LYP results)."

"UV–vis absorption spectra (in the gas phase and ethanol) were examined by TD-DFT using B3LYP, CAM-B3LYP, PBE, PBE0, ωb97X-D, M06-2X, and CIS methods with the 6–311+G(d,p) basis sets for 2OHPhAZ ."

"Among the plethora of functionals, the most often used to simulate the spectra of porphyrins are: B3LYP, CAM-B3LYP, and PBE0 [ xref , xref , xref , xref , xref , xref ]."

"The success of CAM-B3LYP (and other range-separated functionals) is due to the fact that it includes a long-range correction of the exchange potential, which incorporates an increasing fraction of Hartree–Fock (HF) exchange as the interelectronic separation increases."

"All electronic structure calculations were performed with the ORCA software package (version 5.0.3). xref Each set of calculations was conducted using five different density functionals: the hybrid density functionals PBE0 xref , xref and B3LYP, xref , xref as well as the range-separated hybrid functionals LRC-ωPBEh, xref CAM-B3LYP, xref and ωB97X. xref No optimization of the range-separation parameter ω was done."

"As an example of a successful project, the Gene Ontology knowledge base is described, which not only includes terminological knowledge and gene ontology annotations (GOA), but also causal influence models (GO-CAM)."

"We have released version 2 of a comprehensive set of integrated, reviewed annotations for human genes, which we call the “functionome.” We have also dramatically increased the number of GO-CAM models, with over 1500 models of metabolic and signaling pathways, primarily in human, mouse, budding and fission yeast, and fruit fly."

"Over 300 GO-CAM models are currently available for mouse currently ()."

"More precisely, a GO-CAM links the activities (GO MFs) of gene products together by causal relations that specify the effect of one activity on the other."

"All other upstream and downstream molecular functions/biological processes will be modeled in GO-CAM with causal relationships between them and the MAPK cascade."

"GO-CAM models and associated tooling are gradually replacing the traditional GO annotation process within the Alliance for Genomic Resources ( xref ), meaning that future GO annotation will produce an increasingly rich, manually curated KG."

"This restructuring aligned the ontology with emerging principles of ontological clarity promoted by the Common Anatomy Reference Ontology (CARO) and supported compositional reasoning in GO-Causal Activity Modeling (GO-CAM) ( xref ; xref )."

"Hence, a model concept called GO Causal Activity Modeling (GO-CAM) was introduced in 2018 to extend the existing annotation to represent a complex statement that can be scalable and structured [ xref ]."

"Causal networks in GO-CAM models also enable entirely new applications, such as network analysis of genomic data and logical modeling of biological systems."

"The ongoing “Causal Activity Model” project (GO-CAM; [ xref ]) is going one step further in expressiveness with the design of refined properties between GO-BP and GO-MF."

"This study explores the therapeutic potential of andrographolide (AND), a bioactive compound derived from Andrographis paniculata , in mitigating cervical cancer progression using the chick embryo chorioallantoic membrane patient-derived xenograft (CAM-PDX) model."

"Although we have shown transplanting CAM-PDXs is possible, there was no evidence for expansion of the material and given the limitations observed with passaging PDX in mice, researchers should carefully consider whether this is necessary."

"In the CAM-PDX model, AND significantly inhibited tumor growth, primarily by reducing angiogenesis and vessel density."

"In summary, this study successfully established a reproducible CAM-PDX model for drug evaluation and highlighted the potential of AND as a promising therapeutic candidate for cervical cancer, warranting further clinical investigation."

"The option to test treatments in a rapid manner using the CAM-PDX platform, where turnaround time can be weeks instead of months, is highly desirable for cancers with poor prognosis such as mesothelioma, where patients may only survive a few months after their diagnosis."

"We propose that as the model is moderate throughput, drug evaluation can be conducted on first generation (P0) CAM-PDXs, potentially further reducing numbers, as it would remove the standard practice of serial passaging in mice."

"Chorioallantoic membrane patient-derived xenograft (CAM-PDX) models, which combine the strengths of PDX and CAM systems, offer reduced experimental costs alongside enhanced visualization and operability."

"Studies have reported an impressive success rate of 80%–100% in CAM-PDX models across various cancer types, including glioblastoma multiforme, sarcoma, and renal cell carcinoma [ xref , xref ]."

"In this study, we utilized a chick embryo CAM xenograft model (CAM-PDX) to evaluate the therapeutic potential of AND in cervical cancer treatment."

"This study highlights the potential of AND as a therapeutic agent and validates the CAM-PDX model as a reliable platform for further investigation into treatments for cervical cancer."

"Our first aim is to assess the feasibility of CALM-IT as a mobile-paradigm."

"CALM-IT adoption and completion."

"CALM-IT therapists received comprehensive CALM training at the Ferrara coordinating center."

"The development of CALM-IT would allow clinicians and researchers to screen for and track inhibitory control deficits in the community."

"Finally, we assessed clinical relevance through associations of CALM-IT performance and parent- and child-reported symptoms of anxiety, irritability, and ADHD."

"We anticipated deficits in CALM-IT inhibitory control performance would be associated with increased levels of psychopathology."

"CALM-IT feasibility and engagement."

"Only five participants (2.5%) aborted CALM-IT prior to completion."

"Four participants (2%) completed CALM-IT, but their data was not collected due to technical issues."

"Of the remaining participants, eight participants were excluded from analyses of CALM-IT levels 1–5 and 12 participants were excluded from analyses of levels 6–10, due to technical difficulties impacting the number of targets and stars presented."

"The current study compares the salary-related cost of implementing the UB-CAM with and without the skip pattern at the bedside, as administered by the three types of clinicians noted above (see xref )."

"Three-hundred ninety-nine clinicians, including 53 physician hospitalists, 236 nurses, and 110 CNAs, participated, and were briefly trained in the UB-CAM prior to participating in the study."

"As expected, the average per screen cost of administering the UB-CAM ($1.10 for nurses, $3.61 for physicians) were lower than for the UB-CAM-without skip ($1.66 for nurses, $5.59 for physicians)."

"The cost of the simulated CNA-nurse UB-CAM (CNA first step, nurse second step when needed) was slightly lower than the nurse UB-CAM ($0.78 versus $1.10)."

"The lowest cost option for implementing the UB-CAM would be the simulated CNA/Nurse protocol, which would cost $42,339 in a 300-bed hospital."

"The nurse conducted UB-CAM was slightly more expensive at $63,016, and the hospitalist-conducted UB-CAM was much more expensive at $209,407 in a 300-bed hospital."

"The lowest costs per screen were the CNA nurse UB-CAM at 78 cents per screen and nurse UB-CAM at $1.10 per screen."

"Understanding screening costs supports our previous work showing the feasibility, accuracy, and efficiency of the UB-CAM and describing barriers and facilitators of screening for delirium. xref , xref "

"A third limitation is that the CNA-nurse UB-CAM protocol was not explicitly implemented in our study."

"The UB-CAM was generally positively received by all three clinician types."

"To assess test–retest reliability and sensitivity to change, students (n=91) completed the CAM-MYCS at baseline and 1 week after exposure to information on lifestyle-related cancer causes or control information."

"Construct validity was tested by comparing CAM-MYCS scores between cancer experts (n=25) and students (n=91)."

"CAM-MYCS scores improved (fewer myths endorsed) among students exposed to information on cancer causes compared with the control group (p<0.001) and showed high test–retest reliability (r=0.90, p<0.001)."

"With respect to behavioral and lifestyle factors, overweight or obese participants had higher CAM-MYCS median scores than those with normal BMI (p = ."

"Cancer experts reported higher CAM-MYCS scores (fewer myths endorsed) than students (p<0.001)."

"The factor structure of the CAM-MYCS was confirmed in the national sample and internal reliability was high (α=0.86)."

"However, the focus of this study was to examine CC causation myths that were included in the validated CAM-MYCS [ xref ]."

"The CAM-MYCS model was also significant (F = 6.85, p < ."

"Inclusion of the CAM-MYCS alongside items assessing knowledge of actual cancer causes did not affect responses."

"No significant differences were found in CAM-MYCS median scores based on smoking status, physical activity, or alcohol consumption (p = ."

"NMR spectral assignments for the CaM/CaM1 complex were reported previously (BMRB accession number 51222)."

"The NMR-derived structure of CaM/CaM1 reveals intermolecular contacts with the CaM1 residue, F575, that points toward the Ca -occupied N-lobe of CaM bound to CaM1 (Figure 4B)."

"The ITC results indicate that CaM2 binds to the CaM C-lobe with higher affinity than the CaM N-lobe, in contrast to CaM1 that binds to the CaM N-lobe with higher affinity."

"The PDE1 activation process involves Ca 2+ binding to the free CaM region, which results in the activation of the Ca 2+ -CAM and changes in the conformation of the PDE1 structure (Ca 2+ -CaM*-PDE1)."

"These results imply that a single CaM may bind to CNGB1 in which the CaM N-lobe is bound to CaM1 and the CaM C-lobe is bound to CaM2."

"It was shown that GAD activity is induced by two different mechanisms: (i) in intact plant tissue and neutral pH GAD activity is stimulated in a Ca 2+ -dependent manner by binding of calmodulin to the CAM-binding site ( xref ); (ii) after wounding of plant cells the vacuolar content is released and the cytosol is acidified which leads to a Ca 2+ -independent activation of GADs ( xref )."

"NMR-Derived Structures of CaM Bound to the CaM1 Peptide."

"The results of the native gel and fluorescence assays demonstrate that Fh CaM1 binds to the calmodulin binding domain of Fh PMCA."

"The binding of CaM to the CaM1 peptide revealed a biphasic ITC isotherm in which two CaM1 molecules are bound to one CaM molecule at saturation ( xref )."

"A recent NMR structure of CaM bound to a peptide fragment of CaM1 [ xref ] reveals that the Ca 2+ -bound CaM N-lobe binds stably to CaM1 ( xref B), but the Ca 2+ -bound CaM C-lobe binds to CaM1 with much lower affinity, which prevents determination of its structure [ xref ]."

"CD24, CD26, CD44, CD133, CD166, CD34, aldehyde dehydrogenase (ALDH) and Ep‐CAM (also called CD326 or epithelial‐specific antigen/ESA) are some examples of CSC‐specific surface markers."

"Using a panel of monoclonal antibodies to keratinocyte differentiation and proliferation markers, we investigated the association of EP-CAM expression with differentiation-related and/or neoplastic changes in cervical epithelium."

"The pro-metastatic effect of G9a observed in lung cancer is attributed to gene silencing of epithelial cell adhesion molecule (ep-CAM ), enhancing invasion [ xref ]."

"TNFR2 ligands were mainly on CD45 − EP-CAM + lung epithelial cells at steady state (Fig.  xref )."

"To employ the hybrid plan, magnetite nanoparticles were bound to MCF-7 cells through the specific EP-CAM antibodies."

"The embryos were analyzed under a microscope (Olympus CKX53), and images were captured using an Olympus EP50 camera (CAM-EP50) The length and width of embryos after hatching were measured using EPview1.3 software at 96 hpf."

"For flow cytometric analysis of myCAF, iCAF, and apCAF populations, cells were stained for 30 min with anti‐mouse CD31‐FITC (102405, BioLegend), CD45‐APC/Cy7 (557659, BD), CD326 (Ep‐CAM)‐BV421 (118225, BioLegend), PDPN‐APC (127410, BioLegend), Ly6C‐PE (128008, BioLegend), and I‐A/I‐E (MHC‐II)‐PE/Cy7 (107630, BioLegend)."

"Ep‐CAM, as a downstream target gene of Wnt/β‐catenin signal, was positively expressed in non‐responders, which means Ep‐CAM could be a biomarker for IFNAR2‐positive patients resistant to IFN/5‐Fu. xref "

"Western blotting was used to evaluate the expression of ASPP2, LAMB3, HSPB1, EP-CAM, and Ki67 ( xref )."

"Expression of CDCP1 in cell lines is similar to that of the well documented tumour markers CEA and EP-CAM and like these molecules, CDCP1 might prove to be a useful therapeutic target."

"In the recent phase IV, prospective, open-label, exploratory study “Circulating Tumour Cells in Somatuline Autogel Treated NeuroEndocrine Tumors Patients” (CALM-NET) investigating the effect of the administration of 120 mg lanreotide AG every 28 days on CS symptoms, 70.6% of treated patients had an at least 50% reduction in diarrhea episodes and 77.1% in flushing episodes at follow-up [ xref ]."

"To overcome these challenges, we developed a platform named Branched CALM-Net that aims to predict students’ stress levels through dynamic clustering in a personalized manner."

"In the cold-start online learning setting, Branched CALM-Net outperforms other models, attaining an average F1 score of 87% with just 1 week of training data for a new student, which shows it is reliable and effective at predicting stress levels from mobile data."

"The standard version of our proposed method is called the Cross-personal Activity LSTM Multitask Network (CALM-Net), a system designed specifically to deal with inter-subject variability."

"An effect of CTC presence on the effectiveness of somatostatin analog (SSA) treatment has been evaluated in the CALM-NET trial; patients with no detectable CTC might be more likely to respond positively to the treatment [ xref ]."

"CALM-Net learns personalized parameters by considering each subject as a task in a multitask learning structure."

"With shared and individual parts, CALM-Net can learn both the shared dynamics of the data between all subjects as well as personalized characteristics."

"We also propose Branched CALM-Net, a variant of CALM-Net designed to find and share parts of the network among similar subjects."

"Branched CALM-Net combines multitask learning with the Learn-to-Branch technique to efficiently model both personal and population-level characteristics."

"Here we present CAM-Net, a context-aware microbial correlation framework that fundamentally departs from pairwise approaches."

"DSCAM is a member of the cell adhesion molecule (Ig-CAM) immunoglobulin superfamily and is involved in the development of the human central and peripheral nervous system."

"They relate with ECM components through syndecan and integrins molecules and with neighbouring cells through members of the selectin, Ig-CAM families and cadherin, which coordinate signalling and structural functions."

"For example, dysregulation of Phgdh , Eno1 and Gapdh , three functionally linked glycolytic enzymes in module M3 suggests that lower levels of Blimp-1 are impacting on metabolic fitness and the acquisition of Ig-CAM adhesion molecules in M13 such as Icam1 , Bcam and Epcam argues for altered adhesive properties."

"Interactions between the Ig-domains of C-CAM may be the structural basis for the specific homophilic binding."

"On the other hand, eight members are membrane-anchored MMPs, including 4 MMPs with type I transmembrane domain (MMP-14, MMP-15, MMP-16, and MMP-24), two glycosylphosphatidylinositols (GPI)-anchored (M[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Cells at the migratory pole of tumor masses upregulate genes associated with migration and epithelial-to-mesenchymal transition (EMT); vimentin ( VIM ), N-cadherin ( CDH2 ), epidermal growth factor receptor (EGFR) ( ERbB2 ) and downregulate genes associated with cellular adhesion (E-cadherin, Ig-CAM, selectins), in contrast to other cells in the mass which rather may be primed for proliferation and maintenance of the mass. xref , xref Another example reflects the selective pressure of hypoxia, wherein cells in the center of a larger tumor mass undergo programmed necrosis that together facilitate the upregulation of genes associated with angiogenesis; vascular endothelial growth factor ( VEGF) , angiopoietin-1 ( Ang-1 ), and angiopoietin-2 ( Ang-2 ). xref Within this changing environment, it cannot be forgotten that tumor cells are also undergoing immunoediting, with a changing profile that permits the induction of immune tolerance. xref , xref Moreover, while the immunogenicity of hormone-dependent breast cancers, may be regarded as low in comparison to the triple-negative breast cancers, xref that tumors are able to subvert immune cells to use secreted factors for tumor progression is of interest. xref Tumors can recruit a multitude of immune cells including myeloid-derived suppressor cells (MDSCs), which retain the capacity to differentiate into macrophages, granulocytes, and dendritic cells for example. xref Secreted factors from MDSCs including interleukin (IL)-6, transforming growth factor beta (TGF-β), and matrix metalloproteinase 9 are implicated in promoting tumor cell growth, invasion, and angiogenesis. xref , xref MDSCs also induce immune suppression by facilitating tolerance in cytotoxic T cells via nitration or nitrosylation of the T cell receptor or secreted nitric oxide, which prevents the natural killer (NK) cell-FcR mediated activity. xref , xref Cytotoxic function by T cells and NK cells are also controlled by T regulatory cells, which can be subverted by tumor cells to elicit pro-tumor responses. xref Tumor cells also use additional strategies to ensure evasion from cells of the innate and adaptive immune system; for example, downregulation of major histocompatibility complex (MHC) molecules, blockade of activating receptors or shedding of NK activating receptor ligands, inducing T cell anergy, and promoting macrophage differentiation into a pro-tumorigenic M2 phenotype. xref , xref , xref "

"MMP-23 is an MMP that contains N-terminal transmembrane domain type II for anchoring the protein to the cell surface and C-terminal immunoglobulin-like cell adhesion molecule (Ig-CAM) domain ( xref ) to mediate protein–protein and protein–lipid interactions."

"In this study, we revealed that IgSF11, a member of the Ig-CAM family, engages in homophilic interactions and leads to increased osteoclast differentiation through association with PSD-95."

"The Ihog family proteins are type I single-span transmembrane proteins with immunoglobulin (Ig) and fibronectin type III (FNIII) domains, resembling typical cell adhesion molecules in the Ig-CAM (Ig cell adhesion molecule) superfamily."

"Other members of the Ig-CAM family, such as the netrin receptor DCC, the Slit receptor Robo, and neural cell adhesion molecule (N-CAM), have dual roles."

"Compared with group R1, the CAM-CR score in group R2 was higher on day 1 after surgery ( P  < 0.05)."

"The VAS and CAM-CR scores were lower at each postoperative time point in group P than in group C ( p < 0.05)."

"Compared with group P, the CAM-CR score of group R1 was lower on day 1 and day 7 after surgery ( P  < 0.05)."

"Daily assessment was based on patient interview with the CAM‐CR, and delirium was diagnosed by the Delirium Rating Scale (DRS‐R‐98)."

"Among the 166 patients enrolled, after six POD assessments, a total of 53 patients had a CAM-CR score of more than 22 points, which indicated the presence of POD."

"In our study, both CAM‐CR and DRS‐R‐98 scores were used to diagnose perioperative delirium and quantify delirium severity in older hip fracture patients, which provides guidance in our early intervention efforts for preventing and controlling perioperative delirium."

"4AT, CAM, 3D-CAM, CAM-CR, CAM-ICU, CAM-ICU-7, and Nu-DESC were recommended at a level B. The Cronbach’s coefficient of most studies is over 0.8, and the inter-rater reliability of most studies is near or over 0.9, indicating good internal consistency and stability."

"In addition, our comprehensive care program provided preoperative psychological counseling to the patients and used CAM‐CR scores to promptly diagnose delirium."

"Patients in the conventional orthopedic care program were treated with risperidone when they showed signs of delirium and their CAM‐CR score was ≥20."

"Regarding the risk of bias, the overall risk of the seven studies, except for CAM-CR, was relatively low."

"Dastbaravardeh et al. xref proposed a lightweight action recognition framework using CNN with Channel Attention Mechanisms (CNN-CAM) and AE to detect human actions in low-resolution and low-size videos."

"Subsequently, Partial Least Squares (PLS) regression, Convolutional Neural Network (CNN), CNN combined with channel attention mechanism (CNN-CAM), CNN combined with spatial attention mechanism (CNN-SAM), and CNN combined with Convolutional Block Attention Mechanism (CNN-CBAM) models were developed and evaluated for their suitability and stability in predicting wood moisture content."

"Zhang et al. [ xref ] used 3D densely connected convolutional neural network (CAM-CNN) to extract brain MRI multilevel features for classification of Alzheimer's disease and mild cognitive impairment, densely connected difference at different unit levels; 3D dense unit introduces attention mechanism to generate attention maps and sum transformed MRI hierarchical data into more compact high-level; model has high classification prediction accuracy, and classification performance is at the highest level."

"We first proposed the densely connected convolution neural network with connection-wise attention mechanism (short for ‘CAM-CNN’) to learn the multi-level features of MR brain images."

"We proposed a 3D connection-wise-attention-model-based densely connected convolution neural network (CAM-CNN) to learn the multi-level features of brain MR images."

"The proposed CAM-CNN method presented better performance than the popular networks such as ResNet and DenseNet."

"The proposed CAM-CNN model achieved the highest accuracy of 97.35%."

"The area under curve (AUC) of the four networks were all higher than 90%, where AUC of CAM-CNN almost attained 100%."

"DenseNet and CAM-CNN performed well with accuracy of 87.53% and 87.82% respectively, and the AUC of the two models have exceeded 90%."

"The proposed CAM-CNN method again showed the highest accuracy of 78.79% for cMCI vs. ncMCI classification, with AUC of 86.79%."

"The ESP32-CAM can be substituted with a Seeed Studio XIAO camera board, which includes an integrated battery driver so that the lithium-ion battery can instead be connected directly to the board."

"For an ESPlanktoscope using the ESP32-CAM module, which does not provide enough GPIO pins to control the motor directly, the motor driver must be controlled by a second ESP32 microcontroller board connected to the ESP32 camera module, as the ESP32 camera module does not offer enough GPIO pins to control the motor directly; in such a configuration, the ESP32 camera module sends a PWM signal to the second microcontroller in order to set the flow rate of the pump."

"To image small particles such as dust particles, we removed the black plastic cap holding the threaded objective lens provided with the ESP32-CAM module’s camera sensor and instead applied the sample droplet directly to a coverslip placed directly on the sensor window."

"Firmware binaries can be uploaded using either an over-the-air (OTA) mechanism or through the Web Serial-based web page, which provides a selection of customized firmware configurations (e.g. for the ESP32-CAM board, the Seeed Studio Xiao Sense board, Serial Camera, ESPectrophotometer)."

"This study aims to validate the benefits of using a single-chip microprocessor, specifically the ESP32-CAM, as an edge server."

"For instance, a monitoring system that combines the ESP32-CAM and Raspberry Pi with YOLOv8 has been developed to detect and classify objects, such as humans and animals, in the field [ xref ]."

"By leveraging the ESP32-CAM on the edge and YOLOv8 in the cloud, we propose a system that optimizes both latency and accuracy."

"The specific performance of highly constrained hardware like the ESP32-CAM in edge computing scenarios has not been extensively explored."

"Leveraging the ESP32-CAM’s local processing capabilities while selectively offloading complex tasks to the cloud, the proposed system offers a cost-effective and efficient solution for resource-constrained IoT devices."

"The primary objective of this study is to evaluate the integration of edge computing and cloud processing for real-time object recognition using the ESP32-CAM."

"The authors classified this complex as heme oxy I [33] , which is spectrally similar to oxyferrous cytochrome P450-CAM [36] ."

"The hydroxyl group of T252 and WAT901 serves as hydrogen bond donors to the distal oxygen atom of the heme iron-bound dioxygen for wild-type P450-CAM ( Fig. 6 ) [1] ."

"It should be noted, however, that oxyferrous P450-CAM, a heme oxy I-type species, also shows a ferric–superoxide resonance Raman spectral characteristics (ν O–O = 1139–1147 cm −1 ) [38–41] ."

"Recombinant wild-type and T252A mutant P450-CAM proteins were expressed in Escherichia coli [18,30] and purified as reported [31] and stored at −70 °C at ∼100 μM concentrations in 100 mM potassium pho[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"This is because Mb has a neutral histidine as its proximal ligand, while in ferrous P450-CAM or CPO, the proximal ligands is a negatively charged cysteinate."

"Thus, we have first studied the binding of the various camphor analogs to ferric P450-CAM, whose spectral changes are generally more sensitive to differences in substrate-binding mode than those of th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"These spectral features are not seen or are negligible for the camphor- or camphor analog-bound wild-type (even at −40 °C) and T252A mutant (at 4 °C, but seen at −40 °C) enzymes and are likely to be a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"The MCD spectrum of this species is shown in Fig. 4 A. The spectrum is very similar to that previously reported for low-spin ferric P450-CAM [20] and consists of a moderately intense derivative-shaped[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"To avoid appearance of such a P420-type spectral signal, it is important to prepare substrate-bound P450-CAM samples within 1 h after camphor removal at ∼4 °C. The MCD intensity of the derivative-shap[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"The MCD spectrum of ferrous substrate-bound BMP at −55 °C had a sharp derivative-shaped component centered at 556 nm ( Fig. 4 A) that was likely due to a small amount of six-coordinate ferrous species[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"The incidence of treatment-related adverse event (TRAE)-related treatment discontinuation was 13.3% (4/30) in the Cam-NP group and 17.9% (5/28) in the NP group, with no significant difference between the 2 groups ( P  = ."

"Discontinuations in the Cam-NP group were primarily due to bradycardia, pneumonitis, reactive cutaneous capillary endothelial proliferation (RCCEP), and meningitis."

"The ORR in the Cam-NP group was 33.3%, which was significantly higher than the control group ( P  = ."

"Further analysis of patients who underwent at least one computed tomography assessment revealed an ORR of 35.7% in the Cam-NP group, representing a 19.9% improvement over the single-agent cohort, though this did not reach statistical significance ( P  = ."

"The median progression-free survival (mPFS) was 5.62 months in the Cam-NP group, extending the mPFS of the single-agent chemotherapy group by 1.41 months with statistical significance (HR 0.33, P  = ."

"Lastly, there was a noticeable upward trend in median overall survival (mOS) in the Cam-NP group, which reached 9.8 months, extending the mOS of the single-agent group by 2.63 months (HR 0.62, P  = 0.087)."

"Elevated liver aminotransferase levels and skin toxicity were more frequently observed in the Cam-NP group."

"As a result, AC and PC bound and internalized anti-ICAM NP mainly by the CAM pathway (not clathrin pits or caveoli), yet, at a lower level than EC: by 1 h, AC and PC bound 79 and 95 NP/cell vs 200 NP/cell for EC, and internalized 42 and 43 NP/cell vs 110 NP/cell for EC."

"This multicenter, randomized phase II trial evaluated the efficacy of nab-paclitaxel plus camrelizumab (Cam-NP) versus nab-paclitaxel alone (NP) in patients with advanced gastric adenocarcinoma resistant to prior treatment."

"Patients were randomized to receive either Cam-NP or NP until disease progression, intolerable toxicity, or consent withdrawal."

"At the same time, excision wounds treated with uncapped-CAM after the 14 th day showed the presence of a minimal amount of collagen with regeneration and incomplete tissue regeneration, compared with CAM alone, PVA-CAM, PVP-CAM, TSC-CAM or SBD-CAM, as shown in the xref , xref and xref ."

"Histopathological results suggested that the angiogenic response and re-epithelization potential in PVA-CAM were effectively higher than for uncapped-CAM."

"The main reason was the presence of surface coating or capping agents in PVA-CAM, which leads to a better healing capacity, an effective angiogenic response and complete fibroblast and collagen regeneration."

"Of all the optimized formulations being studied in these findings, PVA-CAM possess a predominant antimicrobial potential with an effective wound healing contraction of nearly 69% on the 3 rd day after the wounding process, in contrast with the uncapped-CAM with a value of 20%."

"Similarly on the 14 th day after excision wound injury, treatment with PVA-CAM showed an equal maximum regeneration of fibroblast and collagen and was significantly more effective and angiogenic compared with CAM alone, uncapped-CAM, PVP-CAM, TSC-CAM or SBD-CAM that showed a moderate amount of collagen and fibroblast regeneration, with scant inflammation during their healing process."

"There is a consequent increase in the percentage wound contraction for PVA-CAM with a maximum amount of angiogenic response."

"A possible explanation is as follows: the percentage cumulative release of PVA-CAM is found to be a systematic and controlled release due to their less cytotoxic effects on blood cells, whereas the percentage cumulative release of uncapped-AgNPs is found to have a faster and sudden release profile due to the absence of a capping agent and their high toxicity to blood cells."

"Therefore, due to the lower toxicological effects, controlled release of silver ions and dominant antimicrobial efficacy against selective microorganism, we conclude that PVA-CAM can be considered a more effective polymer for therapeutic benefits in wound healing applications."

"These findings indicate that PVA-CAM have a predominant antibacterial activity and accelerate the healing potential of excision wound models in Wistar albino rats by enhancing angiogenesis in the wounded sections and are comparatively more efficient (among polymers) than uncapped-CAM, SBD-CAM or untreated wounds (the control)."

"The obtained histopathological results show the maximum development of collagen and fibroblast deposition when treated with PVA-CAM and these are relatively higher than for other AgNP formulations."

"To advance the detection of protein sulfhydryl groups, we describe the production of new Rabbit monoclonal antibodies that react with carbamidomethyl-cysteine (CAM-cys), a product of iodoacetamide (IAM) labeling of protein sulfhydryl residues."

"The monoclonal antibodies label a subset of CAM-cys modified peptide sequences and purified proteins (human von Willebrand Factor (gene:vWF), Jagged 1 (gene:JAG1), Laminin subunit alpha 2 (gene:LAMA2), Thrombospondin-2 (gene:TSP2), and Collagen IV (gene:COL4)) but do not recognize specific proteins such as Bovine serum albumin (gene:BSA) and human Thrombospondin-1 (gene:TSP1), Biglycan (gene:BGN) and Decorin (gene:DCN)."

"Scanning mutants of the peptide sequence used to generate the CAM-cys antibodies elucidated residues required for context dependent reactivity."

"Further development of novel CAM-cys monoclonal antibodies in conjunction with other biochemical tools may complement current methods for sulfhydryl detection within specific proteins."

"Moreover, CAM-cys reactive reagents may be useful when there is a need to label subpopulations of proteins."

"To enhance the potential for antibodies to bind to reduced protein on immunoblots, a peptide corresponding to this sequence (CFDPKYGEPKRPPNCARGSCPWDSQL; accession # NP_001138585.2) was generated with a reduced N-terminal anchoring cysteine and two CAM-cys residues in place of the remaining native cysteines."

"Of the antibodies identified by ELISA, we found by dot blotting that two rabbit IgG monoclonals (4E7 and 52H11) bound to the CAM-cys modified MYADML2 peptides but were incapable of binding to peptides lacking CAM-cys modifications (peptide sequences shown in xref ; blots shown in xref [probed with 4E7],1C [with 52H11], and 1D [with secondary antibody alone])."

"We suspected that these MYADML2 non-reactive clones may, in fact produce, antibodies against CAM-cys that were incorporated in the immunizing peptide."

"Breadth of proteins detected by CAM-cys monoclonal antibodies."

"The parameters for the database search were tryptic peptides with one missed cleavage, carbamidomethylation (cys-CAM) as fixed modification, and oxidation (methionine) as variable modification, peptid[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"At the same time, excision wounds treated with uncapped-CAM after the 14 th day showed the presence of a minimal amount of collagen with regeneration and incomplete tissue regeneration, compared with CAM alone, PVA-CAM, PVP-CAM, TSC-CAM or SBD-CAM, as shown in the xref , xref and xref ."

"Histopathological results suggested that the angiogenic response and re-epithelization potential in PVA-CAM were effectively higher than for uncapped-CAM."

"The main reason was the presence of surface coating or capping agents in PVA-CAM, which leads to a better healing capacity, an effective angiogenic response and complete fibroblast and collagen regeneration."

"Of all the optimized formulations being studied in these findings, PVA-CAM possess a predominant antimicrobial potential with an effective wound healing contraction of nearly 69% on the 3 rd day after the wounding process, in contrast with the uncapped-CAM with a value of 20%."

"Similarly on the 14 th day after excision wound injury, treatment with PVA-CAM showed an equal maximum regeneration of fibroblast and collagen and was significantly more effective and angiogenic compared with CAM alone, uncapped-CAM, PVP-CAM, TSC-CAM or SBD-CAM that showed a moderate amount of collagen and fibroblast regeneration, with scant inflammation during their healing process."

"There is a consequent increase in the percentage wound contraction for PVA-CAM with a maximum amount of angiogenic response."

"A possible explanation is as follows: the percentage cumulative release of PVA-CAM is found to be a systematic and controlled release due to their less cytotoxic effects on blood cells, whereas the percentage cumulative release of uncapped-AgNPs is found to have a faster and sudden release profile due to the absence of a capping agent and their high toxicity to blood cells."

"Therefore, due to the lower toxicological effects, controlled release of silver ions and dominant antimicrobial efficacy against selective microorganism, we conclude that PVA-CAM can be considered a more effective polymer for therapeutic benefits in wound healing applications."

"These findings indicate that PVA-CAM have a predominant antibacterial activity and accelerate the healing potential of excision wound models in Wistar albino rats by enhancing angiogenesis in the wounded sections and are comparatively more efficient (among polymers) than uncapped-CAM, SBD-CAM or untreated wounds (the control)."

"The obtained histopathological results show the maximum development of collagen and fibroblast deposition when treated with PVA-CAM and these are relatively higher than for other AgNP formulations."

"To advance the detection of protein sulfhydryl groups, we describe the production of new Rabbit monoclonal antibodies that react with carbamidomethyl-cysteine (CAM-cys), a product of iodoacetamide (IAM) labeling of protein sulfhydryl residues."

"The monoclonal antibodies label a subset of CAM-cys modified peptide sequences and purified proteins (human von Willebrand Factor (gene:vWF), Jagged 1 (gene:JAG1), Laminin subunit alpha 2 (gene:LAMA2), Thrombospondin-2 (gene:TSP2), and Collagen IV (gene:COL4)) but do not recognize specific proteins such as Bovine serum albumin (gene:BSA) and human Thrombospondin-1 (gene:TSP1), Biglycan (gene:BGN) and Decorin (gene:DCN)."

"Scanning mutants of the peptide sequence used to generate the CAM-cys antibodies elucidated residues required for context dependent reactivity."

"Further development of novel CAM-cys monoclonal antibodies in conjunction with other biochemical tools may complement current methods for sulfhydryl detection within specific proteins."

"Moreover, CAM-cys reactive reagents may be useful when there is a need to label subpopulations of proteins."

"To enhance the potential for antibodies to bind to reduced protein on immunoblots, a peptide corresponding to this sequence (CFDPKYGEPKRPPNCARGSCPWDSQL; accession # NP_001138585.2) was generated with a reduced N-terminal anchoring cysteine and two CAM-cys residues in place of the remaining native cysteines."

"Of the antibodies identified by ELISA, we found by dot blotting that two rabbit IgG monoclonals (4E7 and 52H11) bound to the CAM-cys modified MYADML2 peptides but were incapable of binding to peptides lacking CAM-cys modifications (peptide sequences shown in xref ; blots shown in xref [probed with 4E7],1C [with 52H11], and 1D [with secondary antibody alone])."

"We suspected that these MYADML2 non-reactive clones may, in fact produce, antibodies against CAM-cys that were incorporated in the immunizing peptide."

"Breadth of proteins detected by CAM-cys monoclonal antibodies."

"The parameters for the database search were tryptic peptides with one missed cleavage, carbamidomethylation (cys-CAM) as fixed modification, and oxidation (methionine) as variable modification, peptid[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"The CAM-integrin interaction produces a tight adhesion that will finally allow the immobilized leukocyte to transmigrate through the endothelial wall in a process of diapedesis [ 7 ]."

"These experiences suggest that, at least in IBD, targeting the chemokine-chemokine receptor axis is not as effective as targeting integrin-CAM interactions."

"Based on these novel integrin–CAM interactions, and the novel observation that L1 can be expressed on blood vessels under various pathogenic conditions, we propose that L1 may have an expanded and unexpected role in various vascular and thrombogenic processes."

"cCAM integrin-binding active sites are given as amino acid motifs; residues also fought to participate in integrin- CAM interactions are shown in brackets. (Ref."

"Subsequent adhesion and crawling are primarily mediated via the interactions between the members of CAM and β 1 /β 2 integrin families ( xref ), however the specificity of these interactions differs between neutrophils and monocytes ( xref – xref )."

"Besides well-documented integrin–CAM involvement, this process depends first on rearrangement of lymphocyte's cytoskeleton, coordinated by GTPases RAP1 and RhoA and their multiple downstream effectors[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Finally, diapedesis is influenced by HS regarding transendothelial chemokine gradient formation and integrin- CAM interactions, and further enhanced by heparanase-mediated degradation of the endothelial basement membrane."

"Natalizumab, a monoclonal humanized antibody targeting integrin α4, inhibits the transmigration of lymphocytes into the CNS by preventing the interaction of integrin α4β1 with V-CAM expressed on brain vascular endothelial cells."

"Prior to integrin-CAM engagement, there is evidence in autoimmune and allograft transplant models that T cells require a cognate antigen signal, presented by MHC class I or MHC class II expressed on EC, to enter tissues ( xref , xref , xref – xref )."

"This leads to the attraction of B cells to the walls of HEVs, allowing the adhesion of B cells to the endothelium via α4β1 integrins interacting with V-CAM1 ( xref )."

"The system of CAM–LnA exhibited the largest magnitude of density on all of the temperature values, whereas the CIT–LnA HDES system showed the least values for density and PIP–LnA was the intermediate case among the three systems."

"For instance, the CAM–LnA system has two peaks around the wavenumber of 1750 cm –1 ."

"The mass drop percentages from the start of the test until reaching the T onset value were ∼5.0% for the CAM–LnA system and ∼3.0% in the cases of CIT–LnA and PIP–LnA systems."

"For example, the CAM–LnA system shows two prominent peaks around 1750 cm –1 , which correspond to characteristic stretching vibrations from both CAM and LnA, albeit with shifts in intensity due to the change in concentration."

"The best HDES system in selectivity performance was CAM–LnA at the pressure of 2.5 and 5 bar; however, all systems showed similar selectivity values at a higher pressure than 5 bar."

"The CAM–LnA natural HDES system was the most viscose system among the three HDES systems."

"These analyses revealed a strong affinity for CO 2 and N 2 molecules to localize around the HBA–HBD regions of the DES, with CAM–LnA demonstrating the strongest interactions, followed by CIT–LnA."

"Based on these findings, CAM–LnA DES is the best option for low-pressure-separation condition utilization, particularly in the oxy-fuel method and direct air capture technology."

"The equilibrium time was 27, 10, and 4 min at 10, 20, and 30 bar pressures, respectively, in the case of CAM–LnA HDES."

"During the initial 100 s, the cumulative sorption values were ∼62%, ∼77%, and ∼90% at the pressures of 10, 20, and 30 bar, correspondingly, for CAM–LnA DES, whereas the sorption magnitudes by CIT–LnA during the initial 100 s of the experiment were ∼62%, ∼87%, and ∼92%, respectively, at 10, 20, and 30 bar pressures."

"Nevertheless, the probability that CAM–PACs are extruded through another set of efflux systems of RND family, 37 cannot be excluded with absolute certainty."

"This requirement explains our finding that the IC 50 values for CAM and CAM–PACs measured in S. aureus are smaller than those measured in E. coli ( Table 1 )."

"This prompted us to examine the activity of CAM–PACs against three multi-drug resistant (MDR) MRSA isolates, GRE2272, GRE2372, and GRE2691."

"37 Moreover, the PA portion(s) may endow CAM–PACs with the ability to penetrate the bacterial inner membrane, by utilizing the polyamine uptake system, a group of polyamine carriers which pertain to t[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Except for compound 2 , all the rest CAM–PACs of this subgroup competed more efficiently with putrescine and spermidine for the polyamine uptake system, than with spermine."

"This finding tempted us to assume that CAM–PACs are mainly internalized into the cell through the transporter encoded by the pPT104 operon, which constitutes the spermidine-preferential uptake system."

"40 Nevertheless, the most effective member of CAM–PACs in bacteria, compound 4 , was also able to penetrate eukaryotic cells."

"41 The rescue in growth observed when CAM–PACs and exogenous polyamines are simultaneously added in the medium of bacterial culture may arise either due to a competition between them for the polyamin[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"To differentiate between these two possibilities and to confirm that CAM–PACs act on their target after entry into the cells, E. coli cells at an A 560 of 0.020 were grown at 37 °C in M9 medium supple[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"In parallel, cells were grown in the absence of CAM–PACs (control cultures)."

"Since the pixels extracted by PSPNet lack rich contextual information, SA-FFNet xref introduces VH-CAM and UC-PPM to enhance the contextual information of pixels."

"In mutant VH-CAM, Val37, Gly44, Leu45 and Trp47 in framework 2 were substituted by Phe37, Glu44, Arg45 and Gly47, respectively."

"Purification yields of soluble protein from one liter of bacterial culture normalized to an A 550 of 10 were 0.9(±0.1) mg in the VH-W construct, 5(±0.1) mg in VH-CAM, and 8(±0.1) mg in VH-D. All modif[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"To determine the in vitro relative affinity of the isolated VH domains for Vif protein, the amounts of anti-Vif VH, VH-W, VH-CAM and VH-D domains were normalized and analyzed by ELISA."

"To determine the expression levels of single-domains in the reducing environment of the cytoplasm in mammalian cells, VH, VH-W, VH-CAM and VH-D were expressed in 293T cells and cell lyzates were prepa[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"The data in Figure 4 demonstrate that anti-Vif VH-CAM and VH-D antibody fragments were the most abundant intracellularly among the four single-domains."

"Figure 5 shows that anti-Vif VH-CAM and anti-Vif VH-D were the most stable, showing the higher steady-state accumulation and increased half-life, compared to VH and VH-W. Moreover, it should be noted [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"In contrast, the overall steady-state levels of anti-Vif VH and VH-W were much less than VH-CAM and VH-D domains with a relatively short protein half-life of about two hours."

"These results were consistent with our observations using immunofluorescence microscopy where VH and VH-W consistently exhibited weaker fluorescence intensity than VH-CAM and VH-D, suggesting a lower [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Therefore, protein accumulation at steady-state correlates significantly with differences in intracellular protein stability of VH-CAM and VH-D. To provide a qualitative and quantitative measure of th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Furthermore, research has revealed that HCMV encodes UL7 protein, which serves homology to the N-terminal V-like domain of carcinoembryonic antigen-related cell adhesion molecule 1 (CEA-CAM1), a pro-angiogenic factor involved in signal transduction and cellular adhesion."

"To assess the selectivity of the immunosensing platform and ensure that the change in the current is due to antigen binding and not the settling of proteins on the surface, a test of some compounds that may be in negative controls of real samples was conducted, including bovine serum albumin (BSA), carcinoembryonic antigen (CEA-CAM), prostate-specific antigen (PSA), 20S proteasome, ascorbic acid (AA), glucose, and dopamine."

"Contaminating cells, for example inhibitory NK cells [ 27 ], γδ T cells [ 28 ], factors such as CEA-related cellular adhesion molecule-1 (CEA-CAM 1) [ 29 ] and cytokines such as TGFβ [ 30 ], have been[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Our scope, once again, is GPI anchored CEAs: CEA-CAM 6 and CEA-CAM 5, which is also known as CD66e ( xref )."

"CEA-CAM 1 is tumor suppressing through decreasing cell proliferation and metastasis, while CEA-CAM-5 is tumor promoting through inhibiting colon cell differentiation, anoikis and apoptosis ( xref , xref )."

"CEA-CAM 6 oncogenic activity is due to mediating metastasis and resisting apoptosis."

"Increasing metastasis is through CEA-CAM 6 signaling induced SRC activity."

"Carcinoembryonic antigen-related cell adhesion molecule (CEA-CAM)-positive monocytes are associated with inflammation and ILD in SSc patients [ xref , xref , xref , xref ]."

"This complex translocates to the nucleus and binds to CEA-CAM 6 gene promoter, elevating its expression ( xref )."

"Interestingly, CEA-CAM 1 is under expressed in CRC, breast cancer, prostate cancer and hepatoma ( xref )."

"Genomic DNA from CAM-P21 was extracted using the High Pure viral nucleic acid kit (Roche, Mannheim, Germany)."

"The genomic DNA of phage CAM-P21 was completely sequenced with an Illumina HiSeq 4000 platform using the 2 × 150-bp paired-end strategy."

"The three phages (CAM-P21, PC10, and STG2) used in this study were listed in Table 1 ."

"Phage CAM-P21, a member of Siphoviridae family, effective against C. coli, was kindly provided by the Fukuoka City Institute of Health and Environment, Fukuoka."

"CAM-P21 has a linear double-stranded DNA (dsDNA) genome 12,587 bp long with an average GC content of 31.19%."

"Genome of CAM-P21 (GenBank accession number MW462221) does not contain any genes associated to antibiotic resistance, toxins, and virulent factors ( Huang et al., 2021 )."

"No genes related to virulence or resistance were observed according to the genome of CAM-P21, PC10 and STG2."

"In the case of phage CAM-P21, which is specific to C. coli , C. coli CAN10 cells were harvested from 15 mL of the culture by centrifugation at 6500× g for 5 min at 25 °C and resuspended in 1 mL of BHI[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"To 4.8 mL of the cell suspension, 0.1 mL of 5 × 10 8 PFU/mL phage PC10 suspension and 0.1 mL of EDTA solution were added to attain a final concentration of 0.67 mM (0.025%) according to the concentrat[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"In the case of C. coli, to 4.8 mL of BHI broth containing the bacteria at 3 × 10 4 CFU/mL was mixed with 0.1 mL of phage CAM-P21 (1.5 × 10 8 PFU/mL), and the EDTA solutions (0.025%, 0.05%) to attain M[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Furthermore, research has revealed that HCMV encodes UL7 protein, which serves homology to the N-terminal V-like domain of carcinoembryonic antigen-related cell adhesion molecule 1 (CEA-CAM1), a pro-angiogenic factor involved in signal transduction and cellular adhesion."

"To assess the selectivity of the immunosensing platform and ensure that the change in the current is due to antigen binding and not the settling of proteins on the surface, a test of some compounds that may be in negative controls of real samples was conducted, including bovine serum albumin (BSA), carcinoembryonic antigen (CEA-CAM), prostate-specific antigen (PSA), 20S proteasome, ascorbic acid (AA), glucose, and dopamine."

"Contaminating cells, for example inhibitory NK cells [ 27 ], γδ T cells [ 28 ], factors such as CEA-related cellular adhesion molecule-1 (CEA-CAM 1) [ 29 ] and cytokines such as TGFβ [ 30 ], have been[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Our scope, once again, is GPI anchored CEAs: CEA-CAM 6 and CEA-CAM 5, which is also known as CD66e ( xref )."

"CEA-CAM 1 is tumor suppressing through decreasing cell proliferation and metastasis, while CEA-CAM-5 is tumor promoting through inhibiting colon cell differentiation, anoikis and apoptosis ( xref , xref )."

"CEA-CAM 6 oncogenic activity is due to mediating metastasis and resisting apoptosis."

"Increasing metastasis is through CEA-CAM 6 signaling induced SRC activity."

"Carcinoembryonic antigen-related cell adhesion molecule (CEA-CAM)-positive monocytes are associated with inflammation and ILD in SSc patients [ xref , xref , xref , xref ]."

"This complex translocates to the nucleus and binds to CEA-CAM 6 gene promoter, elevating its expression ( xref )."

"Interestingly, CEA-CAM 1 is under expressed in CRC, breast cancer, prostate cancer and hepatoma ( xref )."

"Genomic DNA from CAM-P21 was extracted using the High Pure viral nucleic acid kit (Roche, Mannheim, Germany)."

"The genomic DNA of phage CAM-P21 was completely sequenced with an Illumina HiSeq 4000 platform using the 2 × 150-bp paired-end strategy."

"The three phages (CAM-P21, PC10, and STG2) used in this study were listed in Table 1 ."

"Phage CAM-P21, a member of Siphoviridae family, effective against C. coli, was kindly provided by the Fukuoka City Institute of Health and Environment, Fukuoka."

"CAM-P21 has a linear double-stranded DNA (dsDNA) genome 12,587 bp long with an average GC content of 31.19%."

"Genome of CAM-P21 (GenBank accession number MW462221) does not contain any genes associated to antibiotic resistance, toxins, and virulent factors ( Huang et al., 2021 )."

"No genes related to virulence or resistance were observed according to the genome of CAM-P21, PC10 and STG2."

"In the case of phage CAM-P21, which is specific to C. coli , C. coli CAN10 cells were harvested from 15 mL of the culture by centrifugation at 6500× g for 5 min at 25 °C and resuspended in 1 mL of BHI[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"To 4.8 mL of the cell suspension, 0.1 mL of 5 × 10 8 PFU/mL phage PC10 suspension and 0.1 mL of EDTA solution were added to attain a final concentration of 0.67 mM (0.025%) according to the concentrat[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"In the case of C. coli, to 4.8 mL of BHI broth containing the bacteria at 3 × 10 4 CFU/mL was mixed with 0.1 mL of phage CAM-P21 (1.5 × 10 8 PFU/mL), and the EDTA solutions (0.025%, 0.05%) to attain M[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"These metabolites reflect integrated CAM‐CRF conditions, enhancing the understanding of underlying metabolic profiles in apparently healthy individuals and may represent promising metabolic indicators of systemic health."

"These metabolites reflect integrated CAM‐CRF conditions, enhancing the understanding of underlying metabolic profiles."

"This longstanding interest is based on the strong association of both CRF and CAM with overall health."

"The metabolomic profile of each sex and of each group generated according to the CAM‐CRF profile for both sexes was evaluated."

"In the present study, we identified significant associations between CAM‐CRF profiles and seven metabolites: sebacic acid, ornithine, choline, betaine, N,N ‐dimethylglycine, sarcosine, and glucose."

"Identifying metabolites associated with integrated CAM‐CRF profiles may help characterize healthy physiological states."

"In this context, sebacic acid and ornithine merit attention because they characterize groups with distinct CAM‐CRF profiles based on two PCs, accounting for at least 47.41% of total data variability."

"CAM‐CRF profiles were obtained separately by sex using principal components analysis (PCA) of CAM and CRF."

"Among these, sebacic acid and ornithine emerged as particularly relevant, as they characterize distinct CAM‐CRF profiles and contributed more substantially to overall data variability."

"Interestingly, these metabolites, together with sarcosine, which showed marginal significance in females, p  = 0.019, comprise the mitochondrial arm of the choline degradation pathway, suggesting that mitochondrial disturbances may underlie unfavorable CAM‐CRF profiles."